BLAST of alanyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000005203 (supercontig:LSalAtl2s:LSalAtl2s274:26570:29820:-1 gene:EMLSAG00000005203 transcript:EMLSAT00000005203 description:"maker-LSalAtl2s274-augustus-gene-0.21")

HSP 1 Score: 504.212 bits (1297), Expect = 3.483e-164
Identity = 323/847 (38.13%), Postives = 476/847 (56.20%), Query Frame = 0

Query:   66 NSALDAEE--------YFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGA-----DKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVF--------EEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSP--DEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLID------DAAKVL------DCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQ-VGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEG-----------------------QRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNAN---ASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARAT-GAQALFGEKYDDVVRVVAMG---------P-------NSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKC----RELEKQSAALQSKLASASIDDYLQQVEVINEVNVL 829
            +S+L A+E        +  +++H    SSS +P +DPTLLF NAGM QFK VF+G      DK    RA  SQ+CVRAGGKHNDLE+VG  + HHTFFEMLGN+SFGDYFK+E  +++W+ LTK+  + E++L+V+ F        + D + ++IW D +GVP+ER++    KDNFW+MGDTGPCGPC+EI +D      GG   +   ++D    +EIWNLVF+Q++R +D +L  LPK  +D G+GLER+ + +Q   SNY+ D+F  + +       A+K        +  D+ + + RV+ADHIR+ + +I DG  P N GRGYVLRRI+RR IR   + +  K  F   LV+ +V  +G  +PEL K    I+ ++  E  +FL+TL  G  LLD  I  +DS    KI+ GDV ++LYDTYGFP+DLT  +  ER +++D+ G+E   E                        Q  + +S S+  D  K +    ++     Y  S   A ++AL      V+ I+ G +  I+L+ T FY E GGQ+ D+G +V  N     F+V NVQ   G V  H GTL +G + IG+ V   I+T  R+ +  NH+ TH+L+ AL  VL     QKGSLV +D+LRFDF+  + +T  ++ A+E  VN  +R N E+      +  A+   G +A+F E Y D VRVV++G         P        S+E CGGTH+ R G++ +F ++SE  +A G+RRI ALTG  A  K   QK +L          + N +D     +Q+     +E+ K    L   ++S++I  + ++ E+ N +  L
Sbjct:    2 DSSLKADEIRKLFLDFFSKKKDHTYYHSSSTIPLDDPTLLFINAGMNQFKSVFVGTIDPNNDKAKLVRAVNSQKCVRAGGKHNDLEDVGKDSYHHTFFEMLGNWSFGDYFKKEICEWSWEFLTKELKMPEDRLYVSYFGGNEKTGLDPDLDCKQIWLD-LGVPEERLLPGNMKDNFWEMGDTGPCGPCSEIHFDR----VGGRNAAHLVNQDDPMVLEIWNLVFIQFNREADKSLKLLPKKHIDCGLGLERLVSAIQNKSSNYDTDIFTPIFEAIHERSGASKPYQGKFGDEDPDQIDMAYRVVADHIRTLTIVIADGGRPDNVGRGYVLRRILRRGIRFATEKLNAKPGFFGSLVDVVVSVLGHIFPELTKDPELIKDVINEEEIQFLKTLNRGRKLLDRTIGKMDS----KILPGDVAWRLYDTYGFPIDLTQLMVEERNMTVDLEGYEKAKEKAVLMSQGKGAGTEDRLALDVHSIQELQDKSISTTDDKFKYHYEEKSSDFDSDYEFSGIEATIMALRYEKKFVDSIACGTECGIILDHTSFYAEQGGQIYDEGFIVKVNDDSVEFKVKNVQVRAGYVL-HVGTLGEGELKIGEKVFLQIDTIRRQNVMKNHTGTHILNFALRQVLEGDSDQKGSLVASDKLRFDFTCKKAMTTDQVKAVEMCVNEIIRKNEEIFAKEASLAVAKTIQGLRAVFDETYPDPVRVVSVGIRVEKMEADPTGAAGSVTSVEFCGGTHLKRAGHLQVFVISSEEAIAKGIRRIIALTGPEA--KKATQKAVL----------LQNKVDKVSLVVQEAKLSSKEMVKLITNLSEDISSSTISQW-KKDEMRNSLKAL 825          

BLAST of alanyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000005940 (supercontig:LSalAtl2s:LSalAtl2s320:546129:548925:-1 gene:EMLSAG00000005940 transcript:EMLSAT00000005940 description:"maker-LSalAtl2s320-augustus-gene-4.11")

HSP 1 Score: 267.314 bits (682), Expect = 1.394e-76
Identity = 211/704 (29.97%), Postives = 325/704 (46.16%), Query Frame = 0

Query:  152 MLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSL--------RVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIR---HGYQVGCKVPFMHLLVETLVQEMGEAYPE-----LAKQKGHIEQILQRENERFLETLELGLALLDSHIDSI----------------------DSAK----------SDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNK-------KINVAHLNATEF--------LGYGKSQASAEVVALY-----KGDT-AVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNA--NASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFE-PITDKEILAIEQYVNREVRANYEVTTTLMPIEEAR-----------ATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHV 772
            MLG++SF DYFK++AIQFAW+ LT    + +E L VT F++D E  + W    G+  +++I  G  DNFW+MG TGPCGPCTEI Y                   +  EIWNLVFM+Y R  +G L  L K  VDTGMGLER+ ++L    SNY+ DLF+ + D  +K L   D  + S         R++ADH R  +  + DG+LP      Y LR IIRR ++     +    +V F+  L+  + + +G+ + E       K +  +  +L+ E++   E  +   +LL+   D++                      D+ K            KII+G+  FKLYD+ GF  +     A       D   +E  +   + K +S  +   N+       KI +A      F          Y      + ++ L      K D+  V +++ GD  I++  ++PFY E+GGQ    G++  +   + F V +V    G V+ H GT+  G+    D V+  I+   R     NH+ +HLL+AA+N VL     Q+   V+    +F    F+  ++  EI  IE+  N       ++     PI +A+            +    L GEKY D V ++ + P   E C GTHV  T ++   T+T    V+SG++    LTGQ A+D        LD+V
Sbjct:    1 MLGSWSFNDYFKKDAIQFAWEFLTGPLEIPKEFLNVTYFKDDLETREYWLSQ-GLSPDKIIPKGETDNFWEMGSTGPCGPCTEIHY-------------------KDTEIWNLVFMEYVRRPNGELASLDKKHVDTGMGLERLTSILNGYTSNYDTDLFIPIFDKISK-LSGADPYSGSFDKLQDINYRILADHSRMIATCLSDGLLPD---VSYRLRNIIRRCMKISAESFNSKNQVDFLKELIYCVSESLGDHHREYLESPFLKDEAKLSLLLKFEHDVLKELEKTHKSLLNKLKDAVPFKTLFEKYESELDPSMIGRFYDAFKMYSCNALNNAEPKIISGEFAFKLYDSIGFKSEDIELFALLLDAKYD--PYEFHLYSAKVKEQSKVTTSVNRTNMEILSKITLAPKTDDSFKYKWVQSGFKYQFYIIESNILQLIQISNDKSDSQCVGKLNRGDTGILICEKSPFYSEAGGQAXXTGSITGSLGESVFHVHDVFSLKGVVY-HVGTVHSGTFMRQDLVKLKIDPFKRVTHMQNHTGSHLLNAAMNTVLP-FTTQRSLRVDXTGFKFXACIFKLNLSSSEINDIEEKAN-------QMINNADPIVQAKDKYLDSFIKNSKSKVLTLPGEKYPDKVSMICL-PQFSEPCCGTHVNCTKDVEALTITHIKSVSSGIKSFRCLTGQKAIDARENGLAFLDNV 668          

BLAST of alanyl-trna synthetase vs. L. salmonis genes
Match: EMLSAG00000000400 (supercontig:LSalAtl2s:LSalAtl2s1063:84148:84558:-1 gene:EMLSAG00000000400 transcript:EMLSAT00000000400 description:"augustus_masked-LSalAtl2s1063-processed-gene-0.3")

HSP 1 Score: 63.929 bits (154), Expect = 2.336e-12
Identity = 27/62 (43.55%), Postives = 41/62 (66.13%), Query Frame = 0

Query:    3 LASSSDWQLVNGK--LHFEKRFANFTDAFGFMTKVAIVSEKMNHHPEWSNIYATVIIDLITH 62
            L +   W++V  +  ++ E  F +F +AFGFMT+VA+ ++ MNHHPEW N+Y  V + L TH
Sbjct:   56 LLTKKSWKMVENRDAIYKEFLFKDFNEAFGFMTRVALKADAMNHHPEWFNVYNKVQVTLSTH 117          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|123555227|sp|Q31F91.1|SYA_THICR (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 949.888 bits (2454), Expect = 0.000e+0
Identity = 476/808 (58.91%), Postives = 585/808 (72.40%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQAVAENVAKSHAQGHGMIVVLSAMSGETNRLL 880
            ++F +Q H  V SS +VP NDPTLLFTNAGMVQFK+ FLG + R+Y RAT+ QRC+RAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK EAIQ+AW  LT++ GL EEKLW+TVFEED EAE IW   +GV  ER  R GAKDNFW MGDTGPCGPC+EIFYDHG DVAGGPPGSPDEDGDRYIEIWNLVFMQ+DRS DGTLTPLPKP VDTGMGLER+AAV+Q  H+NY+IDLF  ++  A+++    D +N SLRVIADHIRS +F+++DGVLPSNEGRGYVLRRIIRRAIRHGY++G    F + LV TLV++MGEAYPEL K++ ++E+ L+ E ERF ETLE G+ +L+  I  +    S  +I+G   FKLYDTYGFP+DLT DVARER LS+D  GFE +ME QRA+ARSAS+FG   K  V +  +T F+GY + +A AE+ A++  + +VE+ S G +AI++LNQTPFY ESGGQV D+G+L     SF V N QK  G  F H G +  GSIS+G  + A I+  +R     NHSATHLLHAAL  VLG HV QKGSLV+ +RLRFDFSHFEPI+ +++L IEQ VN  +  N +V    M IE A+A GA ALFGEKY DVVRVV MG  SIELCGGTHV  TG IG F +TSESG+ASGVRRIEA+TG+ A    +  +  L ++A+ LK+          + + + +ELEKQ   LQSKLAS+  DD    V  +N VNVL  ++   D   L  T ++++   E  A          +VL+A+ G+   L+
Sbjct:   12 DFFVKQGHTAVHSSPVVPGNDPTLLFTNAGMVQFKETFLGQETREYKRATSVQRCIRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWTFLTEELGLPEEKLWITVFEEDREAEDIWLKEMGVSAERFSRCGAKDNFWSMGDTGPCGPCSEIFYDHGADVAGGPPGSPDEDGDRYIEIWNLVFMQFDRSEDGTLTPLPKPSVDTGMGLERLAAVMQNQHNNYDIDLFQAIVKKASELTGEKDLANSSLRVIADHIRSCAFMVVDGVLPSNEGRGYVLRRIIRRAIRHGYKLGQTDIFFYQLVPTLVEQMGEAYPELVKEQANVERALKLEEERFAETLENGMKILEEDIAQL----SGSVISGSTAFKLYDTYGFPLDLTADVARERNLSVDEAGFEQEMEAQRARARSASNFGAQSKNKVDYTGSTHFIGYEQDEAEAEIAAIFVDNVSVEKASEGQEAIVILNQTPFYAESGGQVGDQGSLTEGMNSFHVDNCQK-QGAAFLHIGKVTAGSISVGQTILAHIDVKARRASERNHSATHLLHAALRTVLGTHVGQKGSLVQPERLRFDFSHFEPISAEQLLEIEQLVNHNIMLNAQVMMEEMDIEAAKAKGAMALFGEKYGDVVRVVDMGDFSIELCGGTHVNWTGEIGPFRITSESGIASGVRRIEAVTGEAAWQTIYDMEKSLLNIAANLKTDKPQVESKVAQLVTEQKELEKQLKQLQSKLASSQGDDLASSVTEVNGVNVLAAELEGADVNTLRETLDKLRDKLEPAA----------IVLAAVDGDKVSLV 804          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|83305855|sp|Q4K843.1|SYA_PSEF5 (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 932.939 bits (2410), Expect = 0.000e+0
Identity = 461/786 (58.65%), Postives = 564/786 (71.76%), Query Frame = 0

Query:   74 YFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPN-SIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQ 849
            +F EQ H  V+SSSL+P NDPTLLFTNAGM QFKD FLG +KR Y RA +SQ+CVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI FAW  LT  K   L +EKLWVTV+  DDEA  IWT  +GVP ER++RIG       A DNFW MGDTGPCGPCTEIFYDHG D+ GGPPGSP+EDGDRYIEIWN VFMQ++R++DG L PLP P VDTGMGLERI+AVLQ VHSNYEIDLF  L+D AAK + CT+ +  SL+V+ADHIRS  FLI DGVLPSNEGRGYVLRRIIRRA RHG ++G K  F + +V  LV EMGEA+PEL  Q+ HIE++L+ E E+F +TLE GL +L+  +  +  +    ++ GDV+FKLYDTYGFP+DLT D+ARER L+LD  GFE +ME QR +ARSASSFG +    V    ATEF GY  +  SA+VVALYK   +V+ +S G++ ++VL+QTPFY ESGGQ+ D G L   NA F V +  K  G  F H+G L KGS+++G  VE  ++   R   + NHSATHLLHAAL  VLG+HVQQKGSLV++ RLRFDFSHFE I  ++I A+E  VN E+R N  V T    I+ A+  GA ALFGEKY D VRV++MG + S+ELCGG H  RTG+IGL  +TSE GVASGVRRIEA+TG  A+   +  +  L   A+ +K S  N +D     +++ R LEKQ   LQ+K ASA+ DD   Q   +  V VL  ++   D K LL   ++++
Sbjct:   13 FFEEQGHTRVASSSLIPGNDPTLLFTNAGMNQFKDCFLGQEKRAYTRAVSSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAITFAWTFLTSDKWLNLPKEKLWVTVYATDDEAYDIWTKEVGVPAERMVRIGDNKGAPYASDNFWTMGDTGPCGPCTEIFYDHGADIWGGPPGSPEEDGDRYIEIWNNVFMQFNRTADGVLHPLPAPSVDTGMGLERISAVLQHVHSNYEIDLFQSLLDAAAKAIGCTNDAQASLKVVADHIRSCGFLIADGVLPSNEGRGYVLRRIIRRACRHGNKLGAKGSFFYQIVAALVAEMGEAFPELKSQQAHIERVLKAEEEQFAKTLEQGLKILEQDLAELKGS----VVPGDVVFKLYDTYGFPMDLTGDIARERNLTLDEEGFEREMEAQRVRARSASSFGMDYNSLVKVDVATEFTGYHATSGSAKVVALYKEGQSVDMLSEGEEGVVVLDQTPFYAESGGQIGDCGYLQAGNARFDVRDTTKTGG-AFLHHGVLAKGSLTVGAPVETQVDAQVRHATSLNHSATHLLHAALRQVLGEHVQQKGSLVDSQRLRFDFSHFEAIKPEQIKALEDIVNAEIRKNTPVETEETDIDTAKKKGAMALFGEKYGDSVRVLSMGGDFSVELCGGIHANRTGDIGLLKITSEGGVASGVRRIEAVTGAAALAYLNAAEEQLKEAATLVKGSRDNLIDKLSAVLERNRLLEKQLEQLQAKAASAAGDDLSAQAADVKGVKVLAARLDGQDGKALLALVDQLK 793          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830246|sp|A8GA09.2|SYA_SERP5 (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 930.243 bits (2403), Expect = 0.000e+0
Identity = 456/778 (58.61%), Postives = 558/778 (71.72%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKK--FGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVPDNDPTLLFTNAGM QFKDVFLG DKR Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI +AW+LLT +  F L +E+LWVTV+E DDEA  IW   IGVP ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWNLVFMQ++R SDGT+ PLPKP VDTGMGLERIAAVLQ V+SNYEIDLF KLI   AKV   TD  NKSLRVIADHIRS +FL+ DGV PSNEGRGYVLRRIIRRA+RHG  +G K  F + LV  L++ MG A  EL +Q+  +EQ+L+ E ++F  TLE GL LLD  + ++        ++G+  F+LYDTYGFPVDLT DV RERGL +D  GFE  ME QR +AR +S FG +    +    A++F GY   Q  + V AL++    V  I AG++A++VL++TPFYGESGGQV DKG L  ANA F+VS+ QK  GQ   H G L  GS+ + D V+A I+T  R  I  NHSATHLLHAAL   LG+HV QKGSLV    LRFDFSHFE +  ++I  +E  VN+++R N  V T +M +++A+  GA ALFGEKYDD VRV+ MG  S ELCGGTH +RTG+IGLF + SESG A+G+RRIEA+TG  A+   HQQ  LL  VA  +K    N  D     + + R LEK+   L+ + A+        + +++N V +L  ++  V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPDNDPTLLFTNAGMNQFKDVFLGLDKRAYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAISYAWELLTGENWFNLPKERLWVTVYETDDEAFDIWQQQIGVPAERIIRIGDNKGGAFASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNLVFMQFNRQSDGTMLPLPKPSVDTGMGLERIAAVLQHVNSNYEIDLFSKLIAAVAKVTGATDLDNKSLRVIADHIRSCAFLVSDGVTPSNEGRGYVLRRIIRRAVRHGNMLGAKDTFFYKLVAPLIEVMGPAADELKRQQSLVEQVLKTEEDQFARTLERGLTLLDEELANLQG----DTLDGETAFRLYDTYGFPVDLTADVCRERGLKVDEAGFEQAMEAQRRRARESSGFGADYNSMIRVDGASQFSGYDHEQQQSTVTALFRDGQPVNEIHAGEEAVVVLDETPFYGESGGQVGDKGVLKAANADFEVSDTQK-YGQAIGHQGKLSHGSLKVNDRVDAKIDTVRRNRIRLNHSATHLLHAALRQTLGEHVAQKGSLVNDKYLRFDFSHFEAMKPEQIRTVEDLVNQQIRRNLPVQTEVMALDDAKGKGAMALFGEKYDDNVRVLTMGDFSTELCGGTHASRTGDIGLFRILSESGTAAGIRRIEAVTGDGAIATLHQQNDLLQDVAHLVKGDSNNLTDKVRAVLDRTRALEKELQQLKDQQAAQESASLSSKAKMVNGVQLLVSQLDNVEAKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830024|sp|A8ANQ1.1|SYA_CITK8 (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 929.858 bits (2402), Expect = 0.000e+0
Identity = 456/778 (58.61%), Postives = 563/778 (72.37%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKK--FGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP+NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT +  F L +++LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNYEIDLF  LI+  AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  EL +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +    A+EF GY   + + +V AL+    AV+ ISAG +A++VL+QTPFY ESGGQV DKG L  A  +F VS+ QK  GQ   H GTL  GS+ +GDAV+A ++   R  I  NHSATHL+HAAL  +LG HV QKGSLV    LRFDFSH E +   EI A+E  VN ++R N  V T +M +E A+A GA ALFGEKYDD VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H +   L  +A  LK    N  +     +++ R+LEK+   L+ + A+    +   +   IN V +L  ++A V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTGENWFALPKDRLWVTVYETDDEAYEIWEKEVGIPRERIIRIGDNKGAAYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYEIDLFRTLIEAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVVPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEELKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDGASEFKGYDHLELNGKVTALFVDGKAVDAISAGQEAVVVLDQTPFYAESGGQVGDKGELKGAGFAFAVSDTQK-YGQAIGHIGTLSTGSLKVGDAVQADVDDARRARIRLNHSATHLMHAALRQILGTHVAQKGSLVNDKVLRFDFSHNEAMKPSEIRAVEDLVNAQIRRNLPVETNIMDLEAAKAKGAMALFGEKYDDRVRVLSMGDFSTELCGGTHASRTGDIGLFRIVSESGTAAGVRRIEAVTGEGAIATLHAESDRLSDIAQLLKGDSQNLGEKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAVDINGVKLLVSELAGVEPKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|44888327|sp|Q7UBU3.1|SYA_SHIFL (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS >gi|123146773|sp|Q0T1C3.1|SYA_SHIF8 RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 455/778 (58.48%), Postives = 561/778 (72.11%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT  K F L +E+LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNY+IDLF  LI   AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  +L +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +   +A+EF GY   + + +V AL+    AV+ I+AG +A++VL+QTPFY ESGGQV DKG L  AN SF V + QK  GQ   H G L  GS+ +GDAV+A I+   R  I  NHSATHL+HAAL  VLG HV QKGSLV    LRFDFSH E +  +EI A+E  VN ++R N  + T +M +E A+A GA ALFGEKYD+ VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H     L  VA  LK    N  D     +++ R+LEK+   L+ + A+    +   +   +N V +L  +++ V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQK-YGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|123147797|sp|Q0TEI3.1|SYA_ECOL5 (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 454/778 (58.35%), Postives = 561/778 (72.11%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT  K F L +E+LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNY+IDLF  LI   AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  +L +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +   +A+EF GY   + + +V AL+    AV+ I+AG +A++VL+QTPFY ESGGQV DKG L  AN SF V + QK  GQ   H G L  GS+ +GDAV+A ++   R  I  NHSATHL+HAAL  VLG HV QKGSLV    LRFDFSH E +  +EI A+E  VN ++R N  + T +M +E A+A GA ALFGEKYD+ VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H     L  VA  LK    N  D     +++ R+LEK+   L+ + A+    +   +   +N V +L  +++ V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQK-YGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSERLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|123561812|sp|Q32CN1.1|SYA_SHIDS (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 925.235 bits (2390), Expect = 0.000e+0
Identity = 455/778 (58.48%), Postives = 561/778 (72.11%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT  K F L +E+LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNY+IDLF  LI   AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  +L +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +   +A+EF GY   + + +V AL+    AV+ I+AG DA++VL+QTPFY ESGGQV DKG L  AN SF V + QK  GQ   H G L  GS+ +GDAV+A ++   R  I  NHSATHL+HAAL  VLG HV QKGSLV    LRFDFSH E +  +EI A+E  VN ++R N  + T +M +E A+A GA ALFGEKYD+ VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H     L  VA  LK    N  D     +++ R+LEK+   L+ + A+    +   +   +N V +L  +++ V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQDAVVVLDQTPFYAESGGQVGDKGELKGANFSFVVEDTQK-YGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|205830083|sp|A8A3H4.1|SYA_ECOHS (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 924.85 bits (2389), Expect = 0.000e+0
Identity = 454/778 (58.35%), Postives = 561/778 (72.11%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT  K F L +E+LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNY+IDLF  LI   AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  +L +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +   +A+EF GY   + + +V AL+    AV+ I+AG +A++VL+QTPFY ESGGQV DKG L  AN SF V + QK  GQ   H G L  GS+ +GDAV+A ++   R  I  NHSATHL+HAAL  VLG HV QKGSLV    LRFDFSH E +  +EI A+E  VN ++R N  + T +M +E A+A GA ALFGEKYD+ VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H     L  VA  LK    N  D     +++ R+LEK+   L+ + A+    +   +   +N V +L  +++ V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGANFSFAVEDTQK-YGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKML 786          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|20532264|sp|Q9I553.1|SYA_PSEAE (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 924.465 bits (2388), Expect = 0.000e+0
Identity = 449/786 (57.12%), Postives = 569/786 (72.39%), Query Frame = 0

Query:   74 YFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPN-SIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQ 849
            +F E+ H  V+SSSL+P NDPTLLFTNAGM QFKD FLG +KR Y RATTSQ+CVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI +AW+ LT  K   L +EKLWVTV+  DDEA  IWT  +GVP ER++RIG       A DNFW MGDTGPCGPCTEIF+DHG ++ GGPPGSP+EDGDRYIEIWN VFMQ++R++DG + PLP P VDTGMGLER++AVLQ VHSNYEIDLF  L+  +A+ + C +    SL+V+ADHIRS SFLI DGVLPSNEGRGYVLRRIIRRA RHG ++G +  F H +V  LV EMG+A+PEL +Q+ HIE++L+ E E+F +TLE GL +L+  +  +  +    +I G+V+FKLYDTYGFPVDLTND+ARER L++D  GFE +ME QR +AR++S+FG +    V     T FLGY     + ++VAL++   AVER+  G++ ++VL+QTPFY ESGGQV D G L  A   F V +  K  G    H+G + +G++S+G AV+A ++   R+  A NHSATHLLHAAL  VLGDHVQQKGSLV++ RLRFDFSHFE I  +++ A+E  VN E+R N EV T    I+ A+A GA ALFGEKY D VRV++MG + S+ELCGGTHV+RTG+IGLF +TSE GVA+GVRRIEA+TG  A+   +  +  L   AS +K S  N LD     +++ R LEK+   L++K ASA+ DD       I    VL  ++  +D K LL   ++++
Sbjct:   13 FFEEKGHTRVASSSLIPANDPTLLFTNAGMNQFKDCFLGLEKRAYTRATTSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHYAWEFLTGEKWLNLPKEKLWVTVYATDDEAYDIWTKEVGVPAERMVRIGDNKGAPYASDNFWAMGDTGPCGPCTEIFFDHGPEIWGGPPGSPEEDGDRYIEIWNNVFMQFNRTADGVMHPLPAPSVDTGMGLERVSAVLQHVHSNYEIDLFQNLLKASAEAIGCANDDAPSLKVVADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRACRHGNKLGARGTFFHKIVAALVAEMGDAFPELKQQQAHIERVLKTEEEQFAKTLEQGLKILEQDLAELQGS----VIPGNVVFKLYDTYGFPVDLTNDIARERELTIDEDGFEREMEAQRERARASSAFGMDYNSLVKVDGETRFLGYQGVSGAGQIVALFRDGQAVERLEEGEEGVVVLDQTPFYAESGGQVGDSGYLEAAGVRFDVRDTTKAGG-AHLHHGVVARGNLSVGGAVKAEVDASVRQATALNHSATHLLHAALRQVLGDHVQQKGSLVDSQRLRFDFSHFEAIKAEQLKALEDIVNAEIRRNTEVETEETDIDTAKAKGAMALFGEKYGDQVRVLSMGGDFSVELCGGTHVSRTGDIGLFKITSEGGVAAGVRRIEAVTGAAALAYLNGAEEQLKEAASLVKGSRDNLLDKLGALLERNRSLEKELEQLKAKAASAAGDDLSAAAVEIKGAKVLAARLDGLDGKALLALVDQLK 793          

BLAST of alanyl-trna synthetase vs. SwissProt
Match: gi|123558824|sp|Q31X62.1|SYA_SHIBS (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 924.465 bits (2388), Expect = 0.000e+0
Identity = 454/778 (58.35%), Postives = 562/778 (72.24%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLT--KKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            ++F+ + H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR+Y+RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AIQFAW+LLT  K F L +E+LWVTV+E DDEA +IW   +G+P+ER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDRYIEIWN+VFMQ++R +DGT+ PLPKP VDTGMGLERIAAVLQ V+SNY+IDLF  LI   AKV   TD SNKSLRVIADHIRS +FLI DGV+PSNE RGYVLRRIIRRA+RHG  +G K  F + LV  L+  MG A  +L +Q+  +EQ+L+ E E+F  TLE GLALLD  +  +    S   ++G+  F+LYDTYGFPVDLT DV RER + +D  GFE  ME QR +AR AS FG +    +   +A+EF GY   + + +V AL+    AV+ I+AG +A++VL+QTPFY ESGGQV DKG L +AN SF V + QK  GQ   H G L  GS+ +GDAV+A ++   R  I  NHSATHL+HAAL  VLG HV QKGSLV    LRFDFSH E +  +EI A+E  VN ++R N  + T +M +E A+A GA ALFGEKYD+ VRV++MG  S ELCGGTH +RTG+IGLF + SESG A+GVRRIEA+TG+ A+   H     L  VA  LK    N  D     +++ R+LEK+   L+ + A+    +   +   +N V +L  +++ V+ K+L
Sbjct:   14 DFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDLSNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKL----SGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGDKGELKDANFSFAVEDTQK-YGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNLPIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTRQLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKML 786          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1094173275|ref|WP_071160968.1| (alanine--tRNA ligase [Methylomonas sp. LWB] >gi|1093433991|gb|OHX34147.1| alanine--tRNA ligase [Methylomonas sp. LWB])

HSP 1 Score: 952.97 bits (2462), Expect = 0.000e+0
Identity = 464/808 (57.43%), Postives = 577/808 (71.41%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQAVAENVAKSHAQGHGMIVVLSAMSGETNRLL 880
            E+F ++ HA+  SSSLVP NDPTLLFTNAGMVQFKDVFLG +K D++RA TSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK +AI FAW  LT++ G+   KLWVTVF+ED EAE IW + + +   R  RIGAKDNFW MGD GPCGPCTEIFYDHGE VAGGPPGSPD+DGDRYIEIWNLVFMQY+R  DG LTPLP P VDTGMGLERIAAV+Q VHSNYEID+F  L+  AA +   TD SN SLRVIADHIRS +FL+ DGVLPSNEGRGYVLRRI+RRAIRHGY++G +  F + LV  L  EMGEAYPEL K +  +E++L++E ERF ETL  G+ +L++ +  +D      +I GDV+F LYDTYGFPVDLT D ARE  LS+D  GFE +M  QR +AR+   F  +   ++ H + TEF GY     S +V+ L+K    VE +  GD+ +++L+QTPFY ESGGQV D G +     SF+VS+ QK  GQ+F H G + KG I+ G A E S+N D R+    NHSATHLLHAAL  VLG+HV QKGS V A+RLRFDFSHFEP++  +I ++E+ VN ++RAN  V   +M  +EA   GA ALFGEKY D VRV+ MG  S ELCGGTHV RTG+IGLF +  E+GVA+GVRR+EA+TGQ A++    ++  L  +A  LK++   A D   + ++K R LEK+   L+SKLAS++ D+   Q   +N V VL VK+  VD K L    ++++    + A          +VL+A+SG+   L+
Sbjct:   12 EFFRQRGHAVRPSSSLVPGNDPTLLFTNAGMVQFKDVFLGREKVDFSRAATSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKRDAIHFAWDFLTQEMGIPPAKLWVTVFDEDSEAEAIWLEEVRIDPTRFSRIGAKDNFWAMGDVGPCGPCTEIFYDHGEHVAGGPPGSPDDDGDRYIEIWNLVFMQYERDKDGNLTPLPAPSVDTGMGLERIAAVMQGVHSNYEIDIFQNLVKTAAALAGTTDLSNSSLRVIADHIRSCAFLVADGVLPSNEGRGYVLRRIVRRAIRHGYRLGIQDTFFYKLVAPLAAEMGEAYPELVKAQEQVERVLKKEEERFAETLGQGMRILENCVAKLDG----HVIPGDVVFLLYDTYGFPVDLTADFAREHNLSVDHAGFEIEMSAQRDRARAGGQFAADYDQDIRHDSVTEFTGYSHLDGSVQVIGLFKKGQPVESLEVGDEGVVILDQTPFYAESGGQVGDSGVIECTGGSFEVSDTQKQGGQLFLHKGKVVKGVIAEGMACEVSVNADIRKSTERNHSATHLLHAALRAVLGEHVAQKGSQVNAERLRFDFSHFEPMSAAQIASVERIVNEQIRANNAVCAQIMAKDEAVKAGAMALFGEKYGDEVRVLTMGDFSTELCGGTHVQRTGDIGLFKIVGETGVAAGVRRLEAVTGQGALEWIAHREQALLSIAGLLKAAPDKAADKVHQLLEKTRGLEKELEKLKSKLASSAGDEMTSQAVDVNGVKVLAVKLDDVDPKALRDLADQLKNKLGSAA----------LVLAAVSGDKVSLI 805          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|821538967|ref|WP_046860847.1| (alanine--tRNA ligase [Sedimenticola thiotaurini] >gi|821328446|gb|AKH21926.1| alanyl-tRNA synthetase [Sedimenticola thiotaurini])

HSP 1 Score: 952.97 bits (2462), Expect = 0.000e+0
Identity = 477/807 (59.11%), Postives = 582/807 (72.12%), Query Frame = 0

Query:   74 YFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQAVAENVAKSHAQGHGMIVVLSAMSGETNRLL 880
            +F E+ H  V SSSLVP +DPTLLFTNAGMVQFK+ FLG +KRDY RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK EAIQ+AW+ LT+  GL EEKLWVTV+EED EAE IW   + V   R  R GAKDNFW MGDTGPCGPC+EIFYDHG +V GGPPGSP+EDGDRYIEIWNLVFMQ++R   G LTPLP+P VDTGMGLER+AAVLQ VHSNYEIDLF  LI  AA+V  CT+   KSLRVIADHIRS +FL++DGVLPSNEGRGYVLRRIIRRAIRHGY +G K PF H LV  L QEMG+AYPEL +    + ++L+ E ERF ETLE G+ +L+  ID++     DK I G+ +FKLYDTYGFP DLT D+ARERGLSLDMPGFE  ME QR +AR+AS FG  +++ +    ATEF GY K ++ A ++A+++   +VE +   ++ ++VL++TPFY ESGGQV D G L      F V++ QK  G VF H G     S+ +G  V A ++   R   A NHSATHLLHAAL  VLGDHVQQKGSLV A+RLRFDFSHFEP+T ++I  IEQ VN ++R N+ V T +M +E+A+A+GA ALFGEKY D VRV+ MG  S ELCGGTHV   G+IGLF + SE+G+ASGVRRIEA+TG  A+    Q +  L  +A  LKS   +  D   + +++ R LEK+   L+SKLASA+  D       +  V VL  K+  VD K L  T ++++    N   S       +++L+A+SGE   L+
Sbjct:   14 FFIEKGHTEVPSSSLVPHDDPTLLFTNAGMVQFKNTFLGREKRDYTRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLGLPEEKLWVTVYEEDQEAEDIWLKEMKVDPSRFSRCGAKDNFWSMGDTGPCGPCSEIFYDHGPEVWGGPPGSPEEDGDRYIEIWNLVFMQFNRDQSGELTPLPRPSVDTGMGLERLAAVLQHVHSNYEIDLFQHLIKAAAEVTGCTNLEEKSLRVIADHIRSCAFLVVDGVLPSNEGRGYVLRRIIRRAIRHGYMLGMKEPFFHKLVAPLSQEMGDAYPELPRMADQVTRVLRLEEERFAETLEQGMKILEGTIDTL----GDKQIPGETVFKLYDTYGFPTDLTADIARERGLSLDMPGFEQAMEAQRERARAASQFGHAEQVEINLDGATEFTGYDKLESKATIIAMFQDGQSVESLGDDEEGLVVLDRTPFYAESGGQVGDAGVLECGANRFIVTDTQKQGGDVFIHQGRFKGTSLQVGSEVLARVDATRRAATALNHSATHLLHAALQRVLGDHVQQKGSLVNAERLRFDFSHFEPVTIEQIRTIEQMVNEQIRENHMVQTRIMSLEDAKASGAMALFGEKYGDNVRVLRMGDFSTELCGGTHVKAVGDIGLFKIVSETGIASGVRRIEAVTGAAALQWVEQDEDRLQRIADLLKSGRDDVADKVTQLVERSRRLEKELEQLKSKLASAAGTDLASSAIDVAGVKVLAAKLDGVDPKALRDTMDQLK----NKLGS------AVILLAAVSGEKISLV 806          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|652565036|ref|WP_026958531.1| (alanine--tRNA ligase [Aliagarivorans taiwanensis])

HSP 1 Score: 952.97 bits (2462), Expect = 0.000e+0
Identity = 468/812 (57.64%), Postives = 585/812 (72.04%), Query Frame = 0

Query:   74 YFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAK--------DNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQAVAENVAKSHAQGHGMIVVLSAMSGETN 877
            YF ++ H +V+SSSLVP +DPTLLFTNAGM QFKDVFLG+DKR YNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK++AI +AW+ LT+  GL +E+L VTV++ DDEA  IWT  +GVP +RVIRIG K        DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGSP+EDGDR+IEIWN+VFMQ++R +DGT+ PLP P VDTGMGLERIAA+LQ VHSNYEID+F  LI  AA ++  TD  NKSLRVIADHIRS SFLI DGV+PSNEGRGYVLRRIIRRA+RHG ++G    F + LV  L++EMGEAYPEL  Q+  IE++L+ E E+F +TL+ GL +LD  +  +D     K+I G+++FKLYDTYGFP DLT D+ARER LS+D  GFE  M+ QR +A+  S+FG +   N+     T FLGY +  A  +VVALYK   AVE+ ++GD+ ++ L+QTPFY ESGGQ  D G L  AN  F V N  K NG+ F H G L+ G +S+G  VEA +++  R   A NHS THLLHAAL   LG+HV QKGSLVEA RLRFDFSH E ++ K I  IE  VN ++R N+ V T +M ++EA+ +GA ALFGEKYDD VRVV MG  S ELCGGTHV RTG+IG F +T+ESG+A+GVRRIEA+TGQ A+D  H+    ++  AS LK+   + +      + + R LEK+   L++KLA A+  D L QV+ +  V VL   +  V+ K L  T + ++   E+         G+I++  A  G+ N
Sbjct:   15 YFEQKGHQVVASSSLVPHDDPTLLFTNAGMNQFKDVFLGSDKRAYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQDAINYAWEFLTEVAGLPKERLLVTVYQTDDEAFDIWTQQMGVPADRVIRIGDKSEDKPYESDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRFIEIWNVVFMQFNRHADGTMEPLPNPSVDTGMGLERIAAILQGVHSNYEIDIFQNLIKAAADIIGTTDLDNKSLRVIADHIRSCSFLIADGVMPSNEGRGYVLRRIIRRAVRHGNKLGASEGFFNKLVVALIKEMGEAYPELVTQRAMIEKVLRLEEEQFGKTLDRGLQILDEALSELDG----KVIPGELVFKLYDTYGFPADLTGDLARERELSIDEAGFEAAMQAQRERAKQNSNFGVDYNANLVIDGETRFLGYTELDAKGQVVALYKDGEAVEQAASGDELLVALDQTPFYAESGGQAGDHGELALANGRFVVQNTVK-NGKAFVHMGYLEGGELSVGTEVEARVDSRRRWNTALNHSVTHLLHAALRQTLGEHVTQKGSLVEAQRLRFDFSHLEAVSTKSIRQIEHLVNLQIRRNWPVETAVMTLDEAKQSGAMALFGEKYDDDVRVVKMGDFSTELCGGTHVTRTGDIGFFKITAESGIAAGVRRIEAVTGQYAIDLMHKVGEEVEQAASILKTDQFSVIKGVRSAIDRTRLLEKEVEQLKAKLAKAASGDILSQVQELGGVKVLVSALEGVEPKALRTTMDDLKVKLES---------GIILLGVAGEGKVN 812          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|750443905|ref|WP_040725783.1| (alanine--tRNA ligase [Thiomicrorhabdus sp. Kp2])

HSP 1 Score: 952.584 bits (2461), Expect = 0.000e+0
Identity = 474/808 (58.66%), Postives = 587/808 (72.65%), Query Frame = 0

Query:   73 EYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKKFGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIGAKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHLNATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVLLGTTERIQAVAENVAKSHAQGHGMIVVLSAMSGETNRLL 880
            +YF +QNH  V SS +VP NDPTLLFTNAGMVQFK+ FLG ++RDY RAT+ QRC+RAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFK+EAI+FAW  LTK+  L EEKLWVTVF+EDDE   IW   +G+  +R  R GAKDNFWQMGDTGPCGPCTEIFYDHG DVAGGPPGSPDEDGDRYIEIWNLVFMQ+DR+SDGTLTPLPKP VDTGMGLER+AAV+Q+VH+NY+IDLF  ++  AAK+ + TD +N SLRVIADHIRS +F I+DGVLPSNEGRGYVLRRIIRRAIRHGY++G    F + LV  LV++MG+AYPELAK++ ++E+ L+ E ERF ETLE G+ LL+ +I ++D     K I G++ FKLYDTYGFP+DLT DVARE+GL++D  GFE  ME QR +ARSAS+FG      + +   T+FLGY    + +++VA++   T+VE IS G +A+I+L++TPFY ESGGQV D G+L     SF + + QK   + F H G +  GSI++G  + A I+ D R     NHSATHLLHAAL  VLG HVQQKGSLV  DRLRFDFSHFEPI  +++L IE+ VN+ +  N  V T  M IE A+  GA ALFGEKY DVVRVV MG  S+ELCGGTHV  TG IG F L SE+G+ASGVRRIEA+TG+ A    ++    L +VA+++KS          + +   +ELEKQ   LQSK+AS+  DD   Q   + EVN+L  ++   D   L  T ++++      A          +VL+A+ GE   L+
Sbjct:   12 DYFVKQNHTAVHSSPVVPGNDPTLLFTNAGMVQFKETFLGQEQRDYTRATSVQRCIRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQEAIKFAWDFLTKELKLPEEKLWVTVFDEDDETADIWLKEMGISSQRFSRCGAKDNFWQMGDTGPCGPCTEIFYDHGADVAGGPPGSPDEDGDRYIEIWNLVFMQFDRASDGTLTPLPKPSVDTGMGLERLAAVMQDVHNNYDIDLFQAIVKQAAKLTNQTDLTNNSLRVIADHIRSCAFTIVDGVLPSNEGRGYVLRRIIRRAIRHGYKLGMNDAFFYKLVPVLVEQMGDAYPELAKEQANVERALKLEEERFAETLENGMKLLEEYIATMDG----KSIAGEIAFKLYDTYGFPLDLTADVAREKGLTVDEAGFEKSMEAQRERARSASNFGTQSAGKIDYSGETQFLGYNHDDSDSKIVAIFVDGTSVESISEGTEAVIILDKTPFYAESGGQVGDTGSLTEGMNSFHIDSCQK-QAKAFLHMGKVTAGSIAVGQILHAQIDVDGRRSSERNHSATHLLHAALRTVLGTHVQQKGSLVTPDRLRFDFSHFEPIKPEQLLEIEKLVNKNIMLNNPVGTNEMDIESAKEKGAMALFGEKYGDVVRVVDMGDFSVELCGGTHVCSTGQIGPFRLLSETGIASGVRRIEAVTGEGAWAAIYEDDKTLLNVAASVKSDKNQVETKVAQLVADHKELEKQFKQLQSKMASSQGDDLANQAVKVGEVNILAAQLEGADVNTLRETLDKLKDKLAPAA----------IVLAAVDGEKVSLV 804          

BLAST of alanyl-trna synthetase vs. nr
Match: gi|1046294267|ref|WP_065635929.1| (alanine--tRNA ligase [Gilliamella apicola] >gi|1045627152|gb|OCG48027.1| alanine--tRNA ligase [Gilliamella apicola])

HSP 1 Score: 951.814 bits (2459), Expect = 0.000e+0
Identity = 473/791 (59.80%), Postives = 576/791 (72.82%), Query Frame = 0

Query:   61 THKAKNSALDAEEYFNEQNHAIVSSSSLVPDNDPTLLFTNAGMVQFKDVFLGADKRDYNRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKEEAIQFAWQLLTKK--FGLAEEKLWVTVFEEDDEAEKIWTDVIGVPKERVIRIG-------AKDNFWQMGDTGPCGPCTEIFYDHGEDVAGGPPGSPDEDGDRYIEIWNLVFMQYDRSSDGTLTPLPKPCVDTGMGLERIAAVLQEVHSNYEIDLFVKLIDDAAKVLDCTDKSNKSLRVIADHIRSTSFLIIDGVLPSNEGRGYVLRRIIRRAIRHGYQVGCKVPFMHLLVETLVQEMGEAYPELAKQKGHIEQILQRENERFLETLELGLALLDSHIDSIDSAKSDKIINGDVIFKLYDTYGFPVDLTNDVARERGLSLDMPGFETQMEGQRAKARSASSFGDNKKINVAHL-NATEFLGYGKSQASAEVVALYKGDTAVERISAGDDAIIVLNQTPFYGESGGQVADKGTLVNANASFQVSNVQKPNGQVFAHYGTLDKGSISIGDAVEASINTDSRELIAANHSATHLLHAALNDVLGDHVQQKGSLVEADRLRFDFSHFEPITDKEILAIEQYVNREVRANYEVTTTLMPIEEARATGAQALFGEKYDDVVRVVAMGPNSIELCGGTHVARTGNIGLFTLTSESGVASGVRRIEALTGQMAMDKFHQQKTLLDHVASTLKSSVANALDNAEKTMQKCRELEKQSAALQSKLASASIDDYLQQVEVINEVNVLTVKMAQVDNKVL 841
            T + + S LD   +F+++ H +V+SSSLVP NDPTLLFTNAGM QFKDVFLG DKR Y RATTSQRCVRAGGKHNDL+NVGYTARHHTFFEMLGNFSFGDYFK +AI FAW+LLT K  F L +EKL VTV+E DDEA  IW   IG+PKER+IRIG       A DNFWQMGDTGPCGPCTEIFYDHG+ + GGPPGS +EDGDR+IEIWN+VFMQ++R  DGT+ PLPKP VDTGMGLERIAAVLQ V+SNYEIDLF KLI+DAA ++   D  NKSLRVIADHIRS +FLI DGVLPSNEGRGYVLRRIIRRA+RHG+ +G K  F + LV+ L+  MG A  ELA  +  +E  L+ E ++FL+TLE GL LLD  +  I    +  I+ GDV FKLYDTYGFP+DLT DV RE+ +++D  GF   ME QR +AR +SSF  +   NV  L + TEFLGY  ++ S +++A+++    VE I AGD+AII+L++TPFYGESGGQ+ DKG L   N  F V + QK  G+   H G + KGS+ +GD V A+IN + RE I  NHSATHLL AAL  VLG+HV QKGSLV  + LRFDFSH +PIT ++I+ IE  VNR++R N  V   LMPI+EA+  GA ALFGEKYDD+VRV+ MG  SIELCGGTHV RTG+IGLF + SES +ASGVRRIEA +GQ AMD  H +  LL  +   +KS  +  L   E+   + ++LEK    L+++ AS      L Q E IN  N+L  K+  ++ KVL
Sbjct:    4 TAEIRQSFLD---FFHDKGHEVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGVDKRSYTRATTSQRCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKHDAIHFAWELLTSKEWFNLPKEKLTVTVYETDDEAYAIWEKEIGIPKERIIRIGDNKGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHLWGGPPGSAEEDGDRFIEIWNIVFMQFNRHPDGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYEIDLFKKLINDAANIIQTKDLKNKSLRVIADHIRSCAFLISDGVLPSNEGRGYVLRRIIRRAVRHGHMLGAKGIFFYKLVKPLIDVMGSAAVELANNQKQVEDALKLEEKQFLKTLERGLFLLDQELTKI----TGNILPGDVAFKLYDTYGFPLDLTADVCREKNITIDEEGFNQCMEEQRKRARESSSFSVDYS-NVIKLDDKTEFLGYQATETSGKIIAIFRNGQKVESIHAGDEAIIILDKTPFYGESGGQIGDKGLLTAVNVEFTVLDSQK-YGKSIGHIGNIIKGSLKVGDTVTAAINVELRERIRRNHSATHLLQAALQHVLGNHVHQKGSLVNDNYLRFDFSHSQPITQEQIIEIENLVNRQIRRNLPVDIELMPIDEAKNKGAMALFGEKYDDIVRVLTMGDFSIELCGGTHVDRTGDIGLFKIISESSIASGVRRIEATSGQNAMDLIHYESNLLTQIGQIIKSDKSAVLTRIEQLSDQTKQLEKTIEQLKAQKASQESAQLLNQCEKINNTNILIAKLDNIEAKVL 785