BLAST of proclotting enzyme vs. L. salmonis genes
Match: EMLSAG00000008769 (supercontig:LSalAtl2s:LSalAtl2s543:98210:99800:-1 gene:EMLSAG00000008769 transcript:EMLSAT00000008769 description:"maker-LSalAtl2s543-augustus-gene-1.81")

HSP 1 Score: 125.561 bits (314), Expect = 4.479e-35
Identity = 79/224 (35.27%), Postives = 116/224 (51.79%), Query Frame = 0

Query:   51 RIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHIRLGE-HDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFII-GWGSNST-GTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAGNGRIDTCTGDSGGPIQ--SQIDDKWTILGITSFGVGCARE 269
            RIVGG EA     PW   L     S+F   CGG++I    +LSAAHCF +S   ++++G  HD K++S     +   I+ +KIHE W S  ++NDIA+L + E + F   + P+CLP G  GS  L + Q  +  GWG  S     T   LR+  + +++  +     G+     +  DQ+CA     D C  DSGGP+          T+ G+ S+G GCAR+
Sbjct:   14 RIVGGREAYENEIPWQVRLNECDASNFCSNCGGAIIGKRFILSAAHCFYDSKAKNVKVGYIHDKKASS---FSRIYGIEYIKIHEGWHSNLIRNDIALLKVDEDIEFNDGVRPVCLPPG--GSKNLYIGQSSVASGWGLRSEIDNYTSPYLRRTKLTILANDDKRCIKGNTVGGALYKDQLCAFGKNTDACYRDSGGPLTLVGSNHRTCTLAGVISYGNGCARQ 232          

BLAST of proclotting enzyme vs. L. salmonis genes
Match: EMLSAG00000005748 (supercontig:LSalAtl2s:LSalAtl2s309:829439:832849:1 gene:EMLSAG00000005748 transcript:EMLSAT00000005748 description:"maker-LSalAtl2s309-augustus-gene-7.85")

HSP 1 Score: 125.176 bits (313), Expect = 3.658e-34
Identity = 76/247 (30.77%), Postives = 124/247 (50.20%), Query Frame = 0

Query:   41 QSCGFSPFSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNA--LRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAGNGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWI 285
            +SCG    + RI+GG  AR   +PW  +L    +      C GS+I+   VL+AAHCF    I +I++    +         +        IHE++D  +L NDIAI+ +   + F   +  ICLP  +  S   +     + GWG N     ++N+  L+ A ++++     +   G +       + +CA    +D C GDSGGP+ +Q++ K +++G+ S GVGC R  F G+YTRV  ++ WI
Sbjct:   41 ESCG-EVMNSRILGGRNARKFEFPWQVLLA-ICNQTICTECSGSIINKKDVLTAAHCFNIPNIRYIKVSVALLDRRWLTRYTRHYRSSKYVIHEKYDPYTLMNDIAIVHVPVKIRFNPGVQSICLPNKIMSSSF-NGKTATVSGWG-NVMEKNSINSNILKYAKVEVIPNTSVNCTNGALG--IAPKNYLCAYREGVDACQGDSGGPLITQVNGKCSLIGVISGGVGCGRIGFAGLYTRVTEYVDWI 281          

BLAST of proclotting enzyme vs. L. salmonis genes
Match: EMLSAG00000002524 (supercontig:LSalAtl2s:LSalAtl2s149:678275:680326:1 gene:EMLSAG00000002524 transcript:EMLSAT00000002524 description:"maker-LSalAtl2s149-augustus-gene-6.17")

HSP 1 Score: 124.405 bits (311), Expect = 8.792e-34
Identity = 77/268 (28.73%), Postives = 130/268 (48.51%), Query Frame = 0

Query:   42 SCGFSPFSV--------RIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQ------GLAGSDLLSL-PQPFII----GWGSNSTGTGTVN-ALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAGNGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQTNT 289
            +CG S F          RI+GG   +    PW   +        + +CGGS+I   ++L+AAHC  ++   H+++    +  ++  +  +   I+S K+H +W++   +NDIA+L L   + F + + PICLP           +D+    P P ++    GWG+ S      +  +++  + ++   +      D  R  + N  +CA     D C  DSGGP+    ++  ++ GI SFG GCA++ F GVYTRV   L WI+ NT
Sbjct:   41 NCGISKFGAMSCGSREGRIIGGRVVKENEIPWQVNIEMCFFDICERLCGGSVIGRKYILTAAHCILHNT-HHLQVSLGLLNDSNPISDERVYSIESTKLHNDWNAVGAQNDIALLKLDRDIEFDTAVRPICLPSNPDNLFAKKPADIRRCNPGPLMVLCVSGWGTTSEENYESSPVMKRTILRVLENGDDKCRNNDPYRRPLSNKYICAFGTDTDACHIDSGGPLTLVENNTCSLAGIVSFGNGCAKKDFAGVYTRVSAHLDWIRENT 307          

BLAST of proclotting enzyme vs. L. salmonis genes
Match: EMLSAG00000004828 (supercontig:LSalAtl2s:LSalAtl2s254:397245:398159:-1 gene:EMLSAG00000004828 transcript:EMLSAT00000004828 description:"augustus_masked-LSalAtl2s254-processed-gene-4.1")

HSP 1 Score: 120.553 bits (301), Expect = 6.854e-33
Identity = 79/246 (32.11%), Postives = 116/246 (47.15%), Query Frame = 0

Query:   48 FSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHC----FPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAEC-DLAYGDIARVTIGNDQVCAGNGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQTN 288
            F  RIVGG E  P S P+           F   CG S++D   +++AAHC    +P+     +  GEHD+ STS     Q + +  +  HE++ S     D+ +L L   + F   + PI LP+     D   +    + GWG+ S+       L+   + +VS  +C D  YG I    I     CA     D+C GDSGGP+        T++GI S+G GCA   +PGVY +V  F+ WI  +
Sbjct:   38 FGGRIVGGEEVEPNSIPFQISFQTTGGFHF---CGASVMDKDTIITAAHCCDSFYPSE--VQVVAGEHDLFSTS--GDEQKIAVSDITYHEKFASHGTNYDVCLLKLKSSLHFNEKVKPIALPE----KDQEFIGDVVVSGWGTISSSGPPSPVLKAVTVQVVSDEDCSDAYYGSIDETMI-----CAAAPGKDSCQGDSGGPLAQD----GTLVGIVSWGYGCAAPGYPGVYGKVSKFIDWIAEH 263          

BLAST of proclotting enzyme vs. L. salmonis genes
Match: EMLSAG00000000574 (supercontig:LSalAtl2s:LSalAtl2s1096:99490:100302:-1 gene:EMLSAG00000000574 transcript:EMLSAT00000000574 description:"augustus_masked-LSalAtl2s1096-processed-gene-0.2")

HSP 1 Score: 120.553 bits (301), Expect = 9.797e-33
Identity = 84/245 (34.29%), Postives = 124/245 (50.61%), Query Frame = 0

Query:   51 RIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHC-FP-NSAITHI---RLGEHDVKSTSDGAQPQDVLIQ--SVKIHEEWDSQSLKNDIAILTL-SEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIG-WGSNSTGTGT-VNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAGNGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWI 285
            +IVGG +++PG +PW  ++G    +     CGGSL++   +L+AAHC +P NS   H+    LG+H+ +   D   P  V     +V++H  + +    NDIA+L L S+   FT     I + +       L    P ++G WG    G  +  + LR   +  V+   C+ AYG I +       +CAG G  D C GDSGGP+    D K  ++G+ S G GC    FPGVYTRV  +  WI
Sbjct:   27 KIVGGFQSKPGQFPW--IVGLWEKNGISPFCGGSLLNFRWILTAAHCVYPENSETFHLPIAVLGDHNTRDVED---PHKVRTPTCAVEMHPGYSTSKQDNDIALLRLCSDLQQFTKRYFAIDVARP---EQELPDGTPLVVGGWGVLRFGAVSPTDILRAVVVKTVNHDTCNNAYGFITKA-----HICAGTGNKDACQGDSGGPLWLYEDKKPILVGVVSTGRGCGEAQFPGVYTRVSKYFFWI 258          

BLAST of proclotting enzyme vs. L. salmonis genes
Match: EMLSAG00000009833 (supercontig:LSalAtl2s:LSalAtl2s63:1054249:1068721:1 gene:EMLSAG00000009833 transcript:EMLSAT00000009833 description:"maker-LSalAtl2s63-snap-gene-10.55")

HSP 1 Score: 123.25 bits (308), Expect = 1.336e-32
Identity = 72/203 (35.47%), Postives = 116/203 (57.14%), Query Frame = 0

Query:   51 RIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAIT--HIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTV-NALRQAPIDLVSQAECDLAYGDIARVTI--GNDQVCAG--NGRIDTCTGDSGGPI 246
            RIVGG +   GS PW A + + S    ++ CGG+LI    V++AAHC  +++I+  H+RLGE +V+S S+    +D  I+  + H E+ S + +NDIA+L L++ V +   I+P+CLP   +  D     +  +IGWG  + G  T  + L++  + ++S   C   +    R  I   N+ +CAG  +G  D+C GDSGGP+
Sbjct:  226 RIVGGKDTTFGSHPWQAAIVKQSFLSKRISCGGALIGERWVMTAAHCVHSTSISAMHVRLGEWNVRSQSEKFPHEDYAIERKEEHPEYKSATFQNDIALLRLTQDVVYKEHIIPVCLP---SIQDDFEGNKAVVIGWGRTAHGQITTPSVLQEVTVKVLSPKTCQEWFKSNKRKEIIYKNEFICAGYESGGKDSCQGDSGGPL 425          

BLAST of proclotting enzyme vs. L. salmonis genes
Match: EMLSAG00000010925 (supercontig:LSalAtl2s:LSalAtl2s730:283497:284811:1 gene:EMLSAG00000010925 transcript:EMLSAT00000010925 description:"maker-LSalAtl2s730-augustus-gene-3.19")

HSP 1 Score: 116.316 bits (290), Expect = 1.745e-31
Identity = 81/243 (33.33%), Postives = 112/243 (46.09%), Query Frame = 0

Query:   48 FSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHC----FPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAEC-DLAYGDIARVTIGNDQVCAGNGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWI 285
            F  RIVGG E  P S P+           F   CG S++D   +++AAHC    FP      +  GEHD+ STS   Q  DV    +  HE + S     D+ +L L   + F   + PI LP+     D   +    + GWG+ S+       L+   + +VS  +C D  YG I    I     CA      +C GDSGGP+        T++GI S+G GCA   +PGVY +V  F+ WI
Sbjct:   10 FGGRIVGGEEVEPNSIPFQVSFQTTGGFHF---CGASVMDKDTIITAAHCCDSFFPWE--VQVVAGEHDLFSTSGDEQTIDV--SDITYHEMFASHGTNYDVCLLKLKSSLHFNEKVKPIALPE----KDQEFIGDVVVSGWGTISSSGPASPVLKAVTVQVVSDEDCSDAYYGSIDETMI-----CAAAPGKXSCQGDSGGPLAQD----GTLVGIVSWGYGCAAPGYPGVYGKVSKFIDWI 232          

BLAST of proclotting enzyme vs. L. salmonis genes
Match: EMLSAG00000005963 (supercontig:LSalAtl2s:LSalAtl2s321:818470:836551:1 gene:EMLSAG00000005963 transcript:EMLSAT00000005963 description:"maker-LSalAtl2s321-augustus-gene-8.27")

HSP 1 Score: 122.479 bits (306), Expect = 1.836e-31
Identity = 77/238 (32.35%), Postives = 128/238 (53.78%), Query Frame = 0

Query:   57 EARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCF----PNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTST--ILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTV-NALRQAPIDLVSQAECD--LAYGDIAR-VTIGNDQVCAG--NGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFL 282
            E   G +PW   + +  + D   +C GSLID +HV++AAHC     P+  +  +RLGE DV + S+     ++  +++ IHEE+   +L NDIA++ ++E V F+    I P+CLP         +  + F+ GWG ++   G   + L++  + +V  + C+  L    + R  T+    +CAG   G+ D C GD GGP+   ++  W + GI S+G+GC  ++ PGVY +   FL
Sbjct:  993 ETEFGEYPWQVAILKKDEFDNVYLCAGSLIDGSHVITAAHCIRGYNPDEIV--LRLGEWDVNNDSEFYTHIELDAKNLFIHEEFYPGNLYNDIAVIRMTEYVDFSRNRHISPVCLPDSFHN---FAGQRCFVTGWGKDAFLDGNYQHILKEVNLPVVDNSRCENLLRRTKLGRDFTLDKGFLCAGGEEGK-DACKGDGGGPLVCSVNGLWQLAGIVSWGIGCGEQNIPGVYIKSGGFL 1224          

BLAST of proclotting enzyme vs. L. salmonis genes
Match: EMLSAG00000000025 (supercontig:LSalAtl2s:LSalAtl2s1005:6858:7782:-1 gene:EMLSAG00000000025 transcript:EMLSAT00000000025 description:"maker-LSalAtl2s1005-augustus-gene-0.8")

HSP 1 Score: 116.701 bits (291), Expect = 2.385e-31
Identity = 84/243 (34.57%), Postives = 118/243 (48.56%), Query Frame = 0

Query:   48 FSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFP--NSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAEC-DLAYGDIARVTIGNDQ--VCAGNGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWI 285
            F  RIVGG EA P S P+        D  F   CGGS++D   V++AAHC    NS    +  GEHD+ STS     Q + ++ +  HE + S  +  DI +L L   +     + P+ LP+     D        + GWG+ S+   +   LR   + +VS   C D  YG        ND+  +CA     D+C GDSGGP+        T++GI S+G GCA   +PGVY +V  F+ WI
Sbjct:   38 FGGRIVGGEEAEPNSIPFQISFQTTGDFHF---CGGSVMDKDTVITAAHCCDSFNSWEVQVVAGEHDLFSTS--GDEQKIPVKDITYHEMFGSLGMNYDICLLKLKSSLDLNEKVKPVALPK----KDQEFTGDVVVSGWGTISSNGLSSPVLRSVTVQVVSDEYCSDAYYGS-------NDETNICAAAPGKDSCQGDSGGPLAQD----GTLVGIVSWGYGCATPGYPGVYAKVSMFIDWI 260          

BLAST of proclotting enzyme vs. L. salmonis genes
Match: EMLSAG00000012449 (supercontig:LSalAtl2s:LSalAtl2s914:71788:76892:-1 gene:EMLSAG00000012449 transcript:EMLSAT00000012449 description:"maker-LSalAtl2s914-augustus-gene-0.19")

HSP 1 Score: 120.939 bits (302), Expect = 6.466e-31
Identity = 76/245 (31.02%), Postives = 115/245 (46.94%), Query Frame = 0

Query:   49 SVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAIT----HIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIAR--VTIGNDQVCAGNGR-IDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQ 286
            S R+VGG +A PG W W   L    +S  Q +CGG+LI +  VL+AAHC  N        ++R+G+HD+         Q + + +  IH   +SQ+L NDIAIL L         +  +CLP    G+   +     + G+G           +R+A + +V+  EC      +      +     CAG     D C GD GGP+  +    + + G+ S+G GC R   PGVY ++  F+ WI 
Sbjct:  688 SGRVVGGDDASPGEWCWQVAL---INSLNQYLCGGALIGTQWVLTAAHCVTNIVRAGDSIYVRVGDHDLTQKFGSPGAQTLRVATTYIHHNHNSQTLDNDIAILKLHGEADLKEGVCLVCLP--ARGTSQQAGKVCTVTGYGYMGESGPIPLRVREAQVPVVNDNECVRKINAVTEKIFILPASSFCAGGLEGHDACQGDGGGPLVCEDSGYYXLTGLVSWGFGCGRRDVPGVYVKISSFIGWIN 927          

BLAST of proclotting enzyme vs. SwissProt
Match: gi|129688|sp|P21902.1|PCE_TACTR (RecName: Full=Proclotting enzyme; Contains: RecName: Full=Proclotting enzyme light chain; Contains: RecName: Full=Proclotting enzyme heavy chain; Flags: Precursor)

HSP 1 Score: 188.348 bits (477), Expect = 3.806e-56
Identity = 114/303 (37.62%), Postives = 161/303 (53.14%), Query Frame = 0

Query:    6 QSPCGFDRTDYTLCCPGRT-----TTAPTTIPTVPGPDRF------QSCGF-SPFSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSA--------ITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAG--NGRIDTCTGDSGGPIQSQIDD-KWTILGITSFGVGCARESFPGVYTRVDHFLSWI 285
            +S CGF+     +CCP  +     T AP    T   P +       + CG  +  + RI+GG EA  G+WPW   +         V CGG+L+ + HV++A+HC  NSA        +  +RLGEH++ ST D + P D  + SVK HE +   +  NDIAILTL++ V+FT  I PICLP      D L++ +PFI GWG+ +    +   LR+  + +     C  AY     +T  N  +CAG  +G  D C GDSGGP+   +   ++ ++GI SFG  CA   FPGVYT+V  FL WI
Sbjct:   70 ESICGFEGITPKVCCPKSSHVISSTQAPPETTTTERPPKQIPPNLPEVCGIHNTTTTRIIGGREAPIGAWPWMTAVYIKQGGIRSVQCGGALVTNRHVITASHCVVNSAGTDVMPADVFSVRLGEHNLYSTDDDSNPIDFAVTSVKHHEHFVLATYLNDIAILTLNDTVTFTDRIRPICLPYRKLRYDDLAMRKPFITGWGTTAFNGPSSAVLREVQLPIWEHEACRQAYEKDLNIT--NVYMCAGFADGGKDACQGDSGGPMMLPVKTGEFYLIGIVSFGKKCALPGFPGVYTKVTEFLDWI 370          

BLAST of proclotting enzyme vs. SwissProt
Match: gi|657341407|sp|B5U2W0.1|VSP_BOMIG (RecName: Full=Venom serine protease Bi-VSP; AltName: Full=Thrombin-like enzyme; Flags: Precursor)

HSP 1 Score: 172.17 bits (435), Expect = 4.499e-50
Identity = 106/292 (36.30%), Postives = 153/292 (52.40%), Query Frame = 0

Query:    1 LRQAVQSPCGFDRTDYTLCCPGRTTTAPTTIP--TVPGPDRFQSCGFSPFS-VRIVGGTEARPGSWPWAAVLG--RPSDSDFQVV--CGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLP-QGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAG--NGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFL 282
            LRQ+V   C ++  +  +CCP   +     I       P R   CGFS  S  R+VGG  A  G+WPW A LG   P +   + +  CGGSLI S HVL+AAHC   + +  +R+G+ ++    DGA P  + I+S  IH ++ S   K+DIAIL L E V F+  + PICLP +    ++      PF+ GWGS +      + L +  + ++S  EC  +Y   A   + +  +CAG   G  D C GDSGGP+       +  +G+ S+G  CA   +PGVYTRV  +L
Sbjct:   63 LRQSV---CKYENNNPIVCCPNEESREDRGILVGNEYEPLRPPHCGFSNVSHTRVVGGKPAVLGAWPWIAALGFRYPRNPALEPLWKCGGSLISSRHVLTAAHCAEINELYVVRIGDLNLVRNDDGAHPVQIEIESKIIHPDYISGVTKHDIAILKLVEEVPFSEYVYPICLPVEDNLRNNNFERYYPFVAGWGSLAHHGPGSDDLMEVQVPVISNTECKNSYARFAAAHVTDTVLCAGYTQGGKDACQGDSGGPLMLPKKFTFYQIGVVSYGHKCAAAGYPGVYTRVTSYL 351          

BLAST of proclotting enzyme vs. SwissProt
Match: gi|119259|sp|P00774.1|CEL2A_RAT (RecName: Full=Chymotrypsin-like elastase family member 2A; AltName: Full=Elastase-2; AltName: Full=Elastase-2A; Flags: Precursor)

HSP 1 Score: 160.614 bits (405), Expect = 1.229e-46
Identity = 96/259 (37.07%), Postives = 142/259 (54.83%), Query Frame = 0

Query:   42 SCGFSPFSV-----RIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLK--NDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLA--YGDIARVTIGNDQVCA-GNGRIDTCTGDSGGPIQSQIDD-KWTILGITSFG--VGCARESFPGVYTRVDHFLSWIQT 287
            SCG+  + V     R+VGG EA P SWPW   L   S   +   CGGSL+ +  VL+AAHC  NS    + LG H + ++  G+    V +  + +HE+W++Q L   NDIA++ L+ PV+ TS I   CLP   AG+ L +    ++ GWG   T   T + L+Q  + +V  A C  A  +G     ++  + VCA G+G   +C GDSGGP+  Q  + +W + GI SFG  +GC     P V+TRV +++ WI +
Sbjct:   16 SCGYPTYEVQHDVSRVVGGQEASPNSWPWQVSLQYLSSGKWHHTCGGSLVANNWVLTAAHCISNSRTYRVLLGRHSLSTSESGSLA--VQVSKLVVHEKWNAQKLSNGNDIALVKLASPVALTSKIQTACLPP--AGTILPNNYPCYVTGWGRLQTNGATPDVLQQGRLLVVDYATCSSASWWGS----SVKTNMVCAGGDGVTSSCNGDSGGPLNCQASNGQWQVHGIVSFGSTLGCNYPRKPSVFTRVSNYIDWINS 266          

BLAST of proclotting enzyme vs. SwissProt
Match: gi|119255|sp|P08217.1|CEL2A_HUMAN (RecName: Full=Chymotrypsin-like elastase family member 2A; AltName: Full=Elastase-2A; Flags: Precursor)

HSP 1 Score: 159.844 bits (403), Expect = 2.191e-46
Identity = 95/257 (36.96%), Postives = 142/257 (55.25%), Query Frame = 0

Query:   42 SCG---FSPFSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLK--NDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLA--YGDIARVTIGNDQVCA-GNGRIDTCTGDSGGPIQSQIDD-KWTILGITSFG--VGCARESFPGVYTRVDHFLSWIQT 287
            SCG   + P+  R+VGG EARP SWPW   L   S+  +   CGGSLI ++ VL+AAHC  +S    + LG H++     G+    V +  + +H++W+S  +   NDIA+L L+ PVS T  I   CLP   AG+ L +    ++ GWG   T     + L+Q  + +V  A C  +  +G   + ++    +CA G+G I +C GDSGGP+  Q  D +W + GI SFG  +GC     P V+TRV +++ WI +
Sbjct:   16 SCGDPTYPPYVTRVVGGEEARPNSWPWQVSLQYSSNGKWYHTCGGSLIANSWVLTAAHCISSSRTYRVGLGRHNLYVAESGSLA--VSVSKIVVHKDWNSNQISKGNDIALLKLANPVSLTDKIQLACLPP--AGTILPNNYPCYVTGWGRLQTNGAVPDVLQQGRLLVVDYATCSSSAWWGSSVKTSM----ICAGGDGVISSCNGDSGGPLNCQASDGRWQVHGIVSFGSRLGCNYYHKPSVFTRVSNYIDWINS 264          

BLAST of proclotting enzyme vs. SwissProt
Match: gi|75009997|sp|Q7M4I3.1|SP4_BOMPE (RecName: Full=Venom protease; AltName: Allergen=Bom p 4)

HSP 1 Score: 156.762 bits (395), Expect = 1.496e-45
Identity = 89/239 (37.24%), Postives = 129/239 (53.97%), Query Frame = 0

Query:   52 IVGGTEARPGSWPWAAVLG----RPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLP--QGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAG--NGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFL 282
            +VGG  A+ G+WPW   LG    R      +  CGGSL  S HVL+AAHC  + ++  +R+ + ++K   DGA P  + I+S  IH ++      +DIAIL L + VSF+  I PICLP  + L  ++ +    PF+ GWG         +AL +  + +V    C  AY D++   I     CAG   GR D+C GDSGGP+    +  +  +G+ S+G  CA   +PGVYTRV  +L
Sbjct:    1 VVGGKPAKLGAWPWMVALGFHNYRQPKKSPEWKCGGSLRISRHVLTAAHCAIHRSLYVVRIADLNLKRDDDGAHPIQMGIESKLIHPDYVYSEHHDDIAILKLEKDVSFSEYIRPICLPIEESLRNNNFIGY-NPFVAGWGRLRYKGPLSDALMEVQVPVVRNKVCKRAYSDVSDTVI-----CAGYPKGRKDSCQGDSGGPLMIPQESTYYEIGVVSYGHECALPKYPGVYTRVTSYL 233          

BLAST of proclotting enzyme vs. SwissProt
Match: gi|78100736|sp|Q6BEA2.1|PRS27_RAT (RecName: Full=Serine protease 27; AltName: Full=Marapsin; AltName: Full=Pancreasin; Flags: Precursor)

HSP 1 Score: 155.606 bits (392), Expect = 4.165e-44
Identity = 98/263 (37.26%), Postives = 136/263 (51.71%), Query Frame = 0

Query:   36 GPDRFQSCGFSPFSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSA---ITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNA--LRQAPIDLVSQAECDLAY-----GDIARVTIGNDQVCAG--NGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQ 286
            G +  ++CG      R+VGG +A  G WPW   + R + + F   CGGSLI  T VL+AAHCF N++   I  + LG   +K    G     V ++ VK H E+   +   D+A++ L  PV+FT  ILP+CLP         S    ++ GWGS S      N   L++  + L+   +C+L Y      DI   TI +D +CAG   G+ D C GDSGGP+   +D  W   G+ S+G GCAR + PGVY RV     WI 
Sbjct:   22 GAEAMRACGHPRMFNRMVGGEDALEGEWPWQVSIQR-NGAHF---CGGSLIAPTWVLTAAHCFSNTSDISIYQVLLGA--LKLQQPGPHALYVPVKRVKSHPEYQGMASSADVALVELQVPVTFTKYILPVCLPD--PSVVFKSGMNCWVTGWGSPSEQDRLPNPRILQKLAVPLIDTPKCNLLYSKDAEADIQLKTIKDDMLCAGFAEGKKDACKGDSGGPLVCLVDQSWVQAGVISWGEGCARRNRPGVYIRVASHYQWIH 276          

BLAST of proclotting enzyme vs. SwissProt
Match: gi|152032345|sp|Q7M3E1.2|CTRC_BOVIN (RecName: Full=Chymotrypsin-C; AltName: Full=bPTLP; Flags: Precursor)

HSP 1 Score: 152.525 bits (384), Expect = 1.194e-43
Identity = 96/253 (37.94%), Postives = 138/253 (54.55%), Query Frame = 0

Query:   42 SCG---FSP-FSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCA-GNGRIDTCTGDSGGPIQSQIDD-KWTILGITSFGVGCARESF--PGVYTRVDHFLSWIQ 286
            SCG   F P  S R+VGG +A P SWPW   L    D+ ++  CGG+LI   HVL+AAHC  N+    + LG+++++   D A    V + ++ +HE+W+S  ++NDIA++ L+E V  + TI   CLP+   GS L      F+ GWG   T       L+Q    +V  A C  +  D    T+    VCA G+G I  C GDSGGP+  Q ++  W + GI SFG G +  +F  P V+TRV  ++ WI 
Sbjct:   16 SCGAPIFQPNLSARVVGGEDAIPHSWPWQISLQYLRDNTWRHTCGGTLITPNHVLTAAHCISNTLTYRVALGKNNLE-VEDEAGSLYVGVDTIFVHEKWNSFLVRNDIALIKLAETVELSDTIQVACLPE--EGSLLPQDYPCFVTGWGRLYTNGPIAAELQQGLQPVVDYATC--SQRDWWGTTVKETMVCAGGDGVISACNGDSGGPLNCQAENGNWDVRGIVSFGSGLSCNTFKKPTVFTRVSAYIDWIN 263          

BLAST of proclotting enzyme vs. SwissProt
Match: gi|212288098|sp|P08218.2|CEL2B_HUMAN (RecName: Full=Chymotrypsin-like elastase family member 2B; AltName: Full=Elastase-2B; Flags: Precursor)

HSP 1 Score: 152.14 bits (383), Expect = 1.690e-43
Identity = 94/257 (36.58%), Postives = 141/257 (54.86%), Query Frame = 0

Query:   42 SCG---FSPFSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLK--NDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLA--YGDIARVTIGNDQVCA-GNGRIDTCTGDSGGPIQSQIDD-KWTILGITSFG--VGCARESFPGVYTRVDHFLSWIQT 287
            SCG   ++P   R++GG EARP SWPW   L   S+  +   CGGSLI ++ VL+AAHC  +S I  + LG+H++     G+    V +  + +H++W+S  +   NDIA+L L+ PVS T  I   CLP   AG+ L +    ++ GWG   T     + L+Q  + +V  A C  +  +G     T+  + +CA G+G I TC GDSGGP+  Q  D +W + GI S    +GC     P ++TRV ++  WI +
Sbjct:   16 SCGVSTYAPDMSRMLGGEEARPNSWPWQVSLQYSSNGQWYHTCGGSLIANSWVLTAAHCISSSGIYRVMLGQHNLYVAESGSLA--VSVSKIVVHKDWNSDQVSKGNDIALLKLANPVSLTDKIQLACLPP--AGTILPNNYPCYVTGWGRLQTNGALPDDLKQGQLLVVDYATCSSSGWWGS----TVKTNMICAGGDGVICTCNGDSGGPLNCQASDGRWEVHGIGSLTSVLGCNYYYKPSIFTRVSNYNDWINS 264          

BLAST of proclotting enzyme vs. SwissProt
Match: gi|119258|sp|P08419.1|CEL2A_PIG (RecName: Full=Chymotrypsin-like elastase family member 2A; AltName: Full=Elastase-2; AltName: Full=Elastase-2A; Flags: Precursor)

HSP 1 Score: 151.369 bits (381), Expect = 3.459e-43
Identity = 94/257 (36.58%), Postives = 140/257 (54.47%), Query Frame = 0

Query:   42 SCGFS---PFSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLK--NDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLA--YGDIARVTIGNDQVCA-GNGRIDTCTGDSGGPIQSQ-IDDKWTILGITSFG--VGCARESFPGVYTRVDHFLSWIQT 287
            SCG     P   R+VGG +ARP SWPW   L   S   ++  CGG+L+D + VL+AAHC  +S    + LG H + +   G+    V +  + +H++W+S  L   NDIA+L L+ PVS T  I   CLP   AG+ L +    ++ GWG   T   + + L+Q  + +V  A C     +G     T+  + +CA G+G I +C GDSGGP+  Q  + +W + GI SFG  +GC     P V+TRV +++ WI +
Sbjct:   16 SCGLPANLPQLPRVVGGEDARPNSWPWQVSLQYDSSGQWRHTCGGTLVDQSWVLTAAHCISSSRTYRVVLGRHSLSTNEPGSLA--VKVSKLVVHQDWNSNQLSNGNDIALLKLASPVSLTDKIQLGCLPA--AGTILPNNYVCYVTGWGRLQTNGASPDILQQGQLLVVDYATCSKPGWWGS----TVKTNMICAGGDGIISSCNGDSGGPLNCQGANGQWQVHGIVSFGSSLGCNYYHKPSVFTRVSNYIDWINS 264          

BLAST of proclotting enzyme vs. SwissProt
Match: gi|14194504|sp|Q99895.2|CTRC_HUMAN (RecName: Full=Chymotrypsin-C; AltName: Full=Caldecrin; Flags: Precursor)

HSP 1 Score: 150.214 bits (378), Expect = 1.214e-42
Identity = 91/254 (35.83%), Postives = 133/254 (52.36%), Query Frame = 0

Query:   42 SCGFSPF----SVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQP-FIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCA-GNGRIDTCTGDSGGPIQSQIDD-KWTILGITSFGV--GCARESFPGVYTRVDHFLSWIQ 286
            SCG   F    S R+VGG +ARP SWPW   L    +  ++  CGG+LI S  VL+AAHC  N+    + +G+++++   D      V + ++ +H+ W++  L+NDIA++ L+E V  + TI   CLP+      LL    P ++ GWG   T     + L+Q    +V  A C     D     +    VCA G+G I  C GDSGGP+  Q+++  W + GI SFG   GC     P VYTRV  ++ WI 
Sbjct:   16 SCGVPSFPPNLSARVVGGEDARPHSWPWQISLQYLKNDTWRHTCGGTLIASNFVLTAAHCISNTRTYRVAVGKNNLE-VEDEEGSLFVGVDTIHVHKRWNALLLRNDIALIKLAEHVELSDTIQVACLPE---KDSLLPKDYPCYVTGWGRLWTNGPIADKLQQGLQPVVDHATCSRI--DWWGFRVKKTMVCAGGDGVISACNGDSGGPLNCQLENGSWEVFGIVSFGSRRGCNTRKKPVVYTRVSAYIDWIN 263          

BLAST of proclotting enzyme vs. nr
Match: gi|1325326372|ref|XP_023345037.1| (chymotrypsin-like protease CTRL-1 [Eurytemora affinis])

HSP 1 Score: 249.595 bits (636), Expect = 7.267e-77
Identity = 137/292 (46.92%), Postives = 190/292 (65.07%), Query Frame = 0

Query:    4 AVQSPCGFDRTDYTLCCP----GRTTTAPTTIPTVPGPDRFQSCGFSPFSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSA-ITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSL---PQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAGNGRIDTCTGDSGGPIQS-QIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQ 286
            A+++PCGF   D++LCC     G+ +T     PT P P+  Q CG S  S RIVGGT A  G+WPWA VLG+PS S FQV+CGG+L+++  V++AAHCF  S+  THIR G  D+ S S      +V I S+  H +W+  +L  DIAIL LS+P+ +T+ +   CLP      D+ ++   P P ++GWGS + G G    L+QA + +VSQ  C+ AY ++ +V+IG+ ++CAG G  DTC GDSGGP+ S  ++  WT++GITSFGV CAR  FPGVYTRVD +L WI+
Sbjct:  134 AIRAPCGFAGFDFSLCCADQGQGQVSTPSPPQPTPPPPNPNQQCGVSN-SNRIVGGTFANQGTWPWAVVLGKPSGSSFQVICGGTLVNANTVVTAAHCFSGSSDPTHIRAGHVDINSASG----INVQISSITKHPQWNPTTLNADIAILKLSQPLQYTTGVRWACLPDAYQNKDIAAIMTNPDPTVVGWGSTAVGGGAETRLKQAQVPMVSQQTCESAYSNVGQVSIGDAKICAGTGGRDTCNGDSGGPLLSDHLEAGWTLVGITSFGVECARPDFPGVYTRVDKYLDWIR 420          

BLAST of proclotting enzyme vs. nr
Match: gi|1022769646|gb|KZS14121.1| (Chymotrypsin-C [Daphnia magna])

HSP 1 Score: 211.46 bits (537), Expect = 3.233e-62
Identity = 128/313 (40.89%), Postives = 169/313 (53.99%), Query Frame = 0

Query:    1 LRQAVQSPCGFDRTDYTLCCPGRT------TTAP--------TTIPTVPGPDRFQS-------CGFS-PFSVRIVGGTEARPGSWPWAAVLGR--PSDSDFQVVCGGSLIDSTHVLSAAHCFPNSA-ITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLP-QGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAGNGR--IDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWI 285
            L    QS CG+   D  +CC   T      ++AP          IP  P P            CG S   S RIVGG EA PG+WPW A+LG   P+      +CGG+LI S +V++AAHC  N   +  +R+GEH++ +  DG + QDVLI S   HE +DS S +NDIAIL L+  V FT+ + PICLP +    +      QPF+ GWG+ S    +   LR+  I +VSQ  C  +Y +   V +    +CAG GR   D C GDSGGP+     D++ +LG+ SFG  CA   FPGVYTRV H+L WI
Sbjct:  103 LNYLRQSICGYYGYDPKVCCSYSTGVENIDSSAPFFFEESPSPVIPVRPPPHNPSQPSILPARCGHSNATSTRIVGGEEAPPGAWPWIALLGYKDPTTQQVDYLCGGALISSQYVITAAHCVYNKKDLYSVRVGEHELHNDMDGNRHQDVLIASRMPHEGFDSISFQNDIAILKLAARVEFTAEVQPICLPMEPTIRNKNYVKSQPFVAGWGATSFNGPSSVTLREVQIPVVSQESCKESYKNFKTVVVDQSVLCAGLGRGGKDACQGDSGGPLMVPEKDRFYLLGVVSFGYKCAIPGFPGVYTRVPHYLDWI 415          

BLAST of proclotting enzyme vs. nr
Match: gi|926617594|ref|XP_013773969.1| (proclotting enzyme-like [Limulus polyphemus] >gi|926617596|ref|XP_013773970.1| proclotting enzyme-like [Limulus polyphemus] >gi|926617598|ref|XP_013773971.1| proclotting enzyme-like [Limulus polyphemus] >gi|1238845296|ref|XP_022241018.1| proclotting enzyme-like [Limulus polyphemus])

HSP 1 Score: 204.142 bits (518), Expect = 8.061e-60
Identity = 124/313 (39.62%), Postives = 171/313 (54.63%), Query Frame = 0

Query:    1 LRQAVQSPCGFDRTDYTLCCPGRTTTAPTTIPT--------------VPGPDRFQSCGF-SPFSVRIVGGTEARPGSWPWAAVLGRPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAIT--------HIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAGN--GRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQTN 288
            L+QA+   CG++     +CCP   T+ P++ P               +  P     CG  +  S +IVGG EA  G+WPW   +     +     CGG+L+ + HV++A+HC  NSA T         +RLGEH++ ST+D + P D  I+SVK HE+++ ++ KNDIAILT+   V+FT  I PICLP      + L+L  PFI GWG+ +    +   LR+  I +  Q  C  AY     V I N  +CAG   G  D C GDSGGP+    + K+ ++GI SFG GCAR  FPGVYTRV  FL+WI  N
Sbjct:   71 LKQAI---CGYESKIPFVCCP---TSGPSSTPKPEITTNAPTEPTPTLLPPTLPARCGLRNATSTKIVGGREAEVGAWPWMTAVYIKEGNFKSAQCGGALVTNKHVITASHCVVNSAGTDVLPADDFSVRLGEHNLFSTNDSSNPIDFPIKSVKHHEDFELKTYKNDIAILTIDGTVTFTERIHPICLPYNNLRLEDLALRNPFIAGWGTTAFNGPSSAVLREVQIPIWEQENCREAYKK--DVNITNVYMCAGFAVGGKDACQGDSGGPLMLPHESKFYLIGIVSFGKGCARPGFPGVYTRVTEFLNWIAVN 375          

BLAST of proclotting enzyme vs. nr
Match: gi|1227979196|ref|XP_021921184.1| (venom protease-like isoform X1 [Zootermopsis nevadensis])

HSP 1 Score: 201.83 bits (512), Expect = 4.936e-59
Identity = 116/300 (38.67%), Postives = 164/300 (54.67%), Query Frame = 0

Query:    7 SPCGFDRTDYTLCCPGRTTTAPTTIPT----------VPGPDRFQSCGFS--PFSVRIVGGTEARPGSWPWAAVLGRPSDS--DFQVVCGGSLIDSTHVLSAAHCFPNSAITHI-RLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQ-PFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAG--NGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQTN 288
            S CG++ +D  +CCPG  T A    P           V  PD    CG +  P   RI+GG  A+ G+WPW   LG  S S  D + +CGG+LI + HV++A HC       ++ RLGE D++   DGA P D+LI+  K+H  +D ++  ND+AIL L   V++T+ I PICLP       L  + + P+I GWGS      + + L Q  I +VSQA+C  A+   +   I +  +CAG   G  D C GDSGGP+      ++ ++G+ S+G  CA   +PGVYTRV  FL WI T+
Sbjct:   72 SACGYEGSDPKVCCPGTETRATEAEPENDSRTPVKEPVKLPD-VTECGIAGQPDEQRIIGGYPAKLGAWPWLVALGYKSSSSADIKFLCGGALITNRHVITAGHCVHGRKDLYMARLGELDLERDDDGATPIDILIEDHKVHNNYDPRTFVNDVAILRLQNDVNYTNLIRPICLPLAPEIRSLDFVRKFPYIAGWGSVQFNGPSSSQLLQLQIPVVSQAQCKDAFKKFSTAHIDDHVLCAGYAQGGKDACQGDSGGPLMWSKSQRYYLIGVVSYGFRCAEPGYPGVYTRVSAFLDWITTH 370          

BLAST of proclotting enzyme vs. nr
Match: gi|1227979198|ref|XP_021921185.1| (venom protease-like isoform X2 [Zootermopsis nevadensis] >gi|646715184|gb|KDR18888.1| Proclotting enzyme [Zootermopsis nevadensis])

HSP 1 Score: 201.83 bits (512), Expect = 5.563e-59
Identity = 116/300 (38.67%), Postives = 164/300 (54.67%), Query Frame = 0

Query:    7 SPCGFDRTDYTLCCPGRTTTAPTTIPT----------VPGPDRFQSCGFS--PFSVRIVGGTEARPGSWPWAAVLGRPSDS--DFQVVCGGSLIDSTHVLSAAHCFPNSAITHI-RLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQ-PFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAG--NGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQTN 288
            S CG++ +D  +CCPG  T A    P           V  PD    CG +  P   RI+GG  A+ G+WPW   LG  S S  D + +CGG+LI + HV++A HC       ++ RLGE D++   DGA P D+LI+  K+H  +D ++  ND+AIL L   V++T+ I PICLP       L  + + P+I GWGS      + + L Q  I +VSQA+C  A+   +   I +  +CAG   G  D C GDSGGP+      ++ ++G+ S+G  CA   +PGVYTRV  FL WI T+
Sbjct:   68 SACGYEGSDPKVCCPGTETRATEAEPENDSRTPVKEPVKLPD-VTECGIAGQPDEQRIIGGYPAKLGAWPWLVALGYKSSSSADIKFLCGGALITNRHVITAGHCVHGRKDLYMARLGELDLERDDDGATPIDILIEDHKVHNNYDPRTFVNDVAILRLQNDVNYTNLIRPICLPLAPEIRSLDFVRKFPYIAGWGSVQFNGPSSSQLLQLQIPVVSQAQCKDAFKKFSTAHIDDHVLCAGYAQGGKDACQGDSGGPLMWSKSQRYYLIGVVSYGFRCAEPGYPGVYTRVSAFLDWITTH 366          

BLAST of proclotting enzyme vs. nr
Match: gi|1330916403|gb|PNF34238.1| (Venom serine protease Bi-VSP [Cryptotermes secundus])

HSP 1 Score: 199.134 bits (505), Expect = 1.224e-57
Identity = 121/303 (39.93%), Postives = 165/303 (54.46%), Query Frame = 0

Query:    7 SPCGFDRTDYTLCCPG-----------RTTTAPTTIPTVPGPDRFQSCGFSPFSV---RIVGGTEARPGSWPWAAVLGRPSD--SDFQVVCGGSLIDSTHVLSAAHCFPNSAITHI-RLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQG--LAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAG--NGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQTN 288
            S CG++  D  +CCPG             T+AP T   V  PD    CG    +V   R+VGG  A  G+WPW   LG  S   +D + +CG +LI   HV++A HC       ++ RLGE D+K  +DGA P D+LI   K+HEE++  +  NDIAIL L + V+FT+ I PICLP    +   D +    P+I GWGS      + + L+Q  I +VSQA+C  A+   +   I +  +CAG   G  D C GDSGGP+      ++ ++GI SFG  CA   +PGVYTRV  FL WI +N
Sbjct:   91 SACGYEGRDPKVCCPGVATGTTGPPPENNTSAPVT-EYVRLPD-LSECGIVGPAVEDRRVVGGYPAELGAWPWIVALGYKSSPSADVKFLCGSALITHKHVITAGHCVHGRRDLYMARLGELDLKDDNDGATPTDILIVDRKVHEEYNPTTFVNDIAILRLQDAVTFTALIRPICLPLAPEIRTRDFVR-NFPYIAGWGSVQFNGPSSSHLQQLQIPVVSQAQCRDAFKKFSTAHIDDHVMCAGYAQGGKDACQGDSGGPLMWPKSQRFYLIGIVSFGFRCAEPGYPGVYTRVTAFLDWITSN 390          

BLAST of proclotting enzyme vs. nr
Match: gi|1339091731|ref|XP_023707056.1| (venom protease-like [Cryptotermes secundus] >gi|1330916402|gb|PNF34237.1| Venom serine protease Bi-VSP [Cryptotermes secundus])

HSP 1 Score: 198.364 bits (503), Expect = 1.254e-57
Identity = 121/303 (39.93%), Postives = 165/303 (54.46%), Query Frame = 0

Query:    7 SPCGFDRTDYTLCCPG-----------RTTTAPTTIPTVPGPDRFQSCGFSPFSV---RIVGGTEARPGSWPWAAVLGRPSD--SDFQVVCGGSLIDSTHVLSAAHCFPNSAITHI-RLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQG--LAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAG--NGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQTN 288
            S CG++  D  +CCPG             T+AP T   V  PD    CG    +V   R+VGG  A  G+WPW   LG  S   +D + +CG +LI   HV++A HC       ++ RLGE D+K  +DGA P D+LI   K+HEE++  +  NDIAIL L + V+FT+ I PICLP    +   D +    P+I GWGS      + + L+Q  I +VSQA+C  A+   +   I +  +CAG   G  D C GDSGGP+      ++ ++GI SFG  CA   +PGVYTRV  FL WI +N
Sbjct:   74 SACGYEGRDPKVCCPGVATGTTGPPPENNTSAPVT-EYVRLPD-LSECGIVGPAVEDRRVVGGYPAELGAWPWIVALGYKSSPSADVKFLCGSALITHKHVITAGHCVHGRRDLYMARLGELDLKDDNDGATPTDILIVDRKVHEEYNPTTFVNDIAILRLQDAVTFTALIRPICLPLAPEIRTRDFVR-NFPYIAGWGSVQFNGPSSSHLQQLQIPVVSQAQCRDAFKKFSTAHIDDHVMCAGYAQGGKDACQGDSGGPLMWPKSQRFYLIGIVSFGFRCAEPGYPGVYTRVTAFLDWITSN 373          

BLAST of proclotting enzyme vs. nr
Match: gi|820834731|ref|XP_003689688.2| (PREDICTED: LOW QUALITY PROTEIN: venom serine protease Bi-VSP-like [Apis florea])

HSP 1 Score: 195.282 bits (495), Expect = 1.034e-56
Identity = 107/289 (37.02%), Postives = 157/289 (54.33%), Query Frame = 0

Query:    6 QSPCGFDRTDYTLCCPGRTT---TAPTTIPTVPGPDRFQSCGFSPFS-VRIVGGTEARPGSWPWAAVLGRPSDSDFQV--VCGGSLIDSTHVLSAAHCFPNSAITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLP-QGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAG--NGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWI 285
            QS C ++  D  +CCP +      +      + GP     CGF+  S  R+VGG  A+PG+WPW A LG  S +  Q   +CGGSLI + HVL+AAHC  ++ +  +R+G+ D+    DGA P  V I+   IH ++++++  NDIA+L L++ + FT  + PICLP +    ++  +   PF+ GWGS  T     N L +  + ++S  +C  AY       I N  +CA    G  D C GDSGGP+       +  +G+ S+G  CA   FPGVYTRV  FL +I
Sbjct:   61 QSLCRYENNDPFVCCPSKENPGRESKIVKENLYGPLLPPQCGFNNISHTRVVGGIPAKPGAWPWLAALGFRSSNPSQPKWLCGGSLISARHVLTAAHCAIHNNLYLVRIGDLDLSRDDDGAHPIQVEIEDKLIHPDYNTETFVNDIAVLRLAQDIQFTEYVYPICLPVEDNLRNNAFNRNYPFVAGWGSTETRGPASNILLEVQLPVISNEQCKQAYSXFKTAEIDNRVLCAAYRQGGKDACQGDSGGPLMLPQHRFYYQIGVVSYGYKCAEPGFPGVYTRVTTFLDFI 349          

BLAST of proclotting enzyme vs. nr
Match: gi|321463724|gb|EFX74738.1| (hypothetical protein DAPPUDRAFT_226573 [Daphnia pulex])

HSP 1 Score: 197.208 bits (500), Expect = 1.524e-56
Identity = 107/243 (44.03%), Postives = 145/243 (59.67%), Query Frame = 0

Query:   49 SVRIVGGTEARPGSWPWAAVLGRPSDSDFQV--VCGGSLIDSTHVLSAAHCFPNSA-ITHIRLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQG-LAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAGNGR--IDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWI 285
            S RIVGG +A PG+WPW A+LG       QV  +CGG+LI S +V++AAHC  N   +  +R+GEH ++S  DG + QDVLI S   HE +DS S +NDIAIL L+  V FT+ + PICLP   L  +       PF+ GWG+ S    +   LR+  I +V+Q  C  +Y +   V +    +CAG G+   D C GDSGGP+     D++ +LG+ SFG  CA   FPGVYTR+  +L WI
Sbjct:  177 STRIVGGEDAPPGAWPWIALLGYKDPITQQVDHLCGGALISSQYVITAAHCVYNKKDLYSVRVGEHVLQSDMDGNRHQDVLIASRMPHEGFDSVSFQNDIAILKLAVRVEFTAEVQPICLPMDPLIRNKNYVRSNPFVAGWGATSFNGPSSLTLREVQIPVVTQESCKESYKNFKTVVVDQSVLCAGLGKGGKDACQGDSGGPLMIPDKDRFYLLGVVSFGYKCAVPGFPGVYTRIPFYLDWI 419          

BLAST of proclotting enzyme vs. nr
Match: gi|1070141598|ref|XP_018357262.1| (PREDICTED: venom protease [Trachymyrmex septentrionalis] >gi|1070141602|ref|XP_018357263.1| PREDICTED: venom protease [Trachymyrmex septentrionalis])

HSP 1 Score: 192.971 bits (489), Expect = 3.230e-56
Identity = 104/266 (39.10%), Postives = 151/266 (56.77%), Query Frame = 0

Query:   34 VPGPDRFQSCGFSPFSV-RIVGGTEARPGSWPWAAVLG-----RPSDSDFQVVCGGSLIDSTHVLSAAHCFPNSAITHI-------RLGEHDVKSTSDGAQPQDVLIQSVKIHEEWDSQSLKNDIAILTLSEPVSFTSTILPICLPQGLAGSDLLSLPQPFIIGWGSNSTGTGTVNALRQAPIDLVSQAECDLAYGDIARVTIGNDQVCAGNGRIDTCTGDSGGPIQSQIDDKWTILGITSFGVGCARESFPGVYTRVDHFLSWIQ 286
            V  P     CG S   + RIVGGTEA+ G +PW   LG      P       +CGG+LI +TH+L+AAHCF +     I       R+ + D +S++DGA P +VLI+ + +H E+D  S +ND+A+L +   V FTST+LP CLP G   ++ L        GWG    G  + + L +A + ++S  EC  AY +     +    +CA +   D+C GDSGGP+  Q  +K  ++GI S+G+GCAR+ +PGVYTRV  FL +I+
Sbjct:   38 VSDPLSVPQCGISNVQIDRIVGGTEAKLGEFPWIVRLGTVMTFAPGVQWTSWMCGGTLITNTHILTAAHCFSDDFPISIFPEEYIARIADLDFESSNDGAHPVEVLIKEIMLHAEYDDISHENDVAVLKIDREVQFTSTLLPACLPIGNVKNENLDGKMAVTAGWGYTEEGGTSPSTLMEAELSIISNEECQDAYSEQPYAVLDKRTICAASPGKDSCQGDSGGPLMIQNGNKTYVVGIVSWGIGCARQDYPGVYTRVTEFLPFIE 303