BLAST of hypothetical protein DAPPUDRAFT_328037 vs. L. salmonis genes
Match: EMLSAG00000003292 (supercontig:LSalAtl2s:LSalAtl2s180:493171:494827:-1 gene:EMLSAG00000003292 transcript:EMLSAT00000003292 description:"maker-LSalAtl2s180-augustus-gene-4.19")

HSP 1 Score: 410.609 bits (1054), Expect = 1.937e-131
Identity = 201/450 (44.67%), Postives = 306/450 (68.00%), Query Frame = 0

Query:  805 KNLCSQITVEPILFLFSLSHGLYMIIAQTLYIEKVCNVNLNFTREICDNIYVHPDEQIAVQKYVSELQAYNGIIQAIPSIIYALFAGPWSDRYGRRTLIAMACFGYIFNNGVFIINAYWFYELKAEYLLFECLQDCTGGSVCFFLGCYAYISDVSTRKNRTKRLAFLDGLFPIGFFTGMSISGLIKKQWGFIPNFFLGMLAALLAVLYTVFVLKDSQDNRPEEVKAMLKEREAASTTTEPAR---KRNVCAQLFDLENLKASFRTTFKKRDNGMGSALTLLIFTFVVEIFMTHGRGATMFLYFRKEFAWTETQFGQFMALFGVVGLFTQYVAVPFLTDTCKIHD---TLLGLGGVVGSGINLLIMALVRHDEWL-YLGCVVAFLGPTITTSCRSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPGMFLIISACFY 1247
            + +   IT+EP++FLF LSHGLY I  Q+LYI KVC VNLN +  ICD+IY H + Q  VQKYVS+LQAYN  +Q +P++IY+LFAGPWSD  GR+ +I ++CFGY+FNN ++++N  +F+ELKAEYLLFECLQD TGG V FF  C++YI+D++   +RTKR+AFLDG+FPIGF  G+ +SG+IKK+ GF   F L ++ +  ++LYT+F +K+ +++  ++V     +    ++TT+ A    K+    ++F +++ +A  ++T K+R++ +   L  LI  F++E+F+  G+G+ MFLY RK F WTE  F QFM +FG+ GL +QY  +PFL+   K  D    L+ +GG + +G     M    +  WL Y+  +V+FLG  I+T  RSL++KC+   E+G VFS++ + QA + + A P +  +YKAT+D FPG+  ++++C Y
Sbjct:    9 RYILDNITLEPVVFLFGLSHGLYSITVQSLYISKVCAVNLNHSLTICDDIYQHEEIQTEVQKYVSQLQAYNFFLQGLPAVIYSLFAGPWSDINGRKAIIVLSCFGYVFNNVIYLLNTVFFFELKAEYLLFECLQDFTGGYVAFFFACHSYITDITDDSSRTKRMAFLDGIFPIGFLMGLGLSGIIKKKLGFTYCFSLAIVTSWSSILYTIFFIKE-RNSSEDQV-----DNTDDNSTTQSANNKVKKEPIWKMFKIQHFQAGVKSTLKRREHDLKKYLLFLILIFMLELFLYGGKGSVMFLYLRKRFKWTEIDFSQFMIVFGIQGLVSQYFVIPFLSHKLKWRDGTIILIAVGGNIIAG----FMFTFANVSWLVYVAGIVSFLGAAISTLLRSLISKCIESDEIGKVFSVIASGQAVIFMIASPIYSLVYKATLDFFPGLVFLVTSCLY 448          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. L. salmonis genes
Match: EMLSAG00000002356 (supercontig:LSalAtl2s:LSalAtl2s144:172548:174259:-1 gene:EMLSAG00000002356 transcript:EMLSAT00000002356 description:"maker-LSalAtl2s144-snap-gene-1.24")

HSP 1 Score: 341.658 bits (875), Expect = 4.935e-107
Identity = 178/389 (45.76%), Postives = 240/389 (61.70%), Query Frame = 0

Query:  865 QKYVSELQAYNGIIQAIPSIIYALFAGPWSDRYGRRTLIAMACFGYIFNNGVFIINAYWFYELKAEYLLFECLQDCTGGSVCFFLGCYAYISDVSTRKNRTKRLAFLDGLFPIGFFTGMSISGLIKKQWGFIPNFFLGMLAALLAVLYTVFVLKDSQDNRPEEV------KAMLKEREAASTTTEPARKRNVCAQLFDLENLKASFRTTFKKRDNGMGSALTLLIFTFVVEIFMTHGRGATMFLYFRKEFAWTETQFGQFMALFGVVGLFTQYVAVPFLTDTCKIHDTLLGLGGVVGSGINLLIMALVRHDEWLYLGCVVAFLGPTITTSCRSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPGMFLIISACFY 1247
            QK VS LQAYNGI+Q IP + YALFAGPWSD+ GRR L+ ++ F Y                                          +YISD++  K RTKRLAFLDGLFP+G+F GM++SG+IK + GF+ NF  G+  AL ++ Y +F +KDSQ  RP+EV      K  LKER+ +       + +N+C  +FD++N+K +F+ TFK+R  G    L LL+  FV+EIF+ +G+G  MFLY RK+F W E  FGQ+ A+FG +GL TQYV VPFL +  K+HD+ + +  + G  +  +I A  + D  LY   ++AFLG  ITT  RSLVTKCV PFEVG VFS+M A QA VPL A P + F+YK+T++TFPG FL+ +A  Y
Sbjct:    1 QKDVSTLQAYNGILQGIPPVFYALFAGPWSDKNGRRVLMMLSIFAY------------------------------------------SYISDITDVKTRTKRLAFLDGLFPVGWFIGMALSGVIKNKLGFLYNFGGGLTLALCSLFYVIFFVKDSQQMRPKEVTESEEYKTHLKERDLSH------KNKNICQSIFDVQNVKEAFQCTFKERPYGKRKYLVLLVVCFVIEIFLMNGKGPIMFLYLRKQFKWDEVLFGQYSAIFGFLGLATQYVVVPFLINRLKLHDSTIAIIAIAGCIVQQIIAAYAKVDWLLYFAGIIAFLGVCITTVSRSLVTKCVSPFEVGKVFSVMAACQALVPLVASPVYSFMYKSTIETFPGAFLLFTAGIY 341          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. L. salmonis genes
Match: EMLSAG00000004300 (supercontig:LSalAtl2s:LSalAtl2s225:250476:256507:1 gene:EMLSAG00000004300 transcript:EMLSAT00000004300 description:"maker-LSalAtl2s225-augustus-gene-2.22")

HSP 1 Score: 319.701 bits (818), Expect = 3.831e-93
Identity = 264/825 (32.00%), Postives = 402/825 (48.73%), Query Frame = 0

Query:    7 VAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFI--QLTPSLFELVKSGPAFGAQSTLKYVVLGGEPFPKAIPSPAPAHLRYFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSDTHVSLTS-----------QGEIVVSSATRICRVHPGQGTQHTEKGYSVFTGDLGEAQCTGDGQQAILF---HTRCQNFVKLQGRRISFPELAQDLSQAL-GCPFHCVLKEDRRVLGFHSQ--DIDPSR---LSHLPPILRGVELVKVPRFPITRHGKIDLKLLLEWKEETHRKKVGLNVAK-----LWRQLTGIDPVVDSNFVRDGGDSILAVEFINEIEDATNNPSVQSLLELLLNGQFGEFQDQIMARIGDSSILAQSRVKEHHLNSCSDMDISRTPLDRSADHTQISILGRGFRRGPIRSTIDIGSTDLKVLWSVDLNKCIDATPLIVKDKEDTVIFIGSHSGLFAAIHMDSGRTRWTRNLGDRIESSANCSPDGRLIFIGCYDCAMYCLDARSGDILWSFKTGGIIKSIPAVDGERVIFGSYDHFVYCLNSITGTLLWAKNIARSSILTKALTLVDGSVIACTLSGHVSKRDTQEGANMWTTQLDGPIFSS--PLCIGTWGY--FATAKGLLSKLCLASSELVWTFDAQGPIFSSLVPSGSDILLGTHACEV----LCVDSTSGLCRWRVKHTDRVFATPLLTSSQMCVSLSTDGQIIVSKDGAIVKMVYFPVKDRLQGGFFSSPLAHNGQIFQNLKDT 796
            + Y I TSGTT  +K V  S +S+++NV+DF   F   S     +  SPP FDPFY++IL +V    PI   +  +++ + ++ + I     + I  Q+TP+L+  + S       S     VLGGE FP    S  P H  Y+N +GVTEMSCWQS+++++   S    PI     NL+S+T V L             +G IVVSS  R C            +G S    D  E    G  ++  L+        N VK+ G+R +  E+ + L   L    FH    E+   L   ++  ++  S+   ++     +   +++KV + P   +GK++ K  L     T RK+   N+ K     LW + TGI P   +NF+ DGGDS  ++ F+ E+     +P +   +E++LN  F    D + A  G   ++  S+ + + L   S +             TQ S   RGF    + +   I S +L   W +DL KCIDA+ L+V   +D + ++GSHSG+F +  +  G  +W+  L DRIESSA  S   R + +GCY+ A+Y ++ +SG I WS+ T G+IKS P +  E VIFGSYD+FVY L+  TGTL W  N   S ILT    + DG     +L+G   K +T  G   W  +L  PIFSS   L IGT     F  A G+L  +   SS+++WTF A+  IFSS+   G+ I  G+H   +    +C        +  + H   +++TP     Q    +S DG+ +V +   +   V  PV     G  FSSP+  + ++    +D 
Sbjct:  139 ILYAITTSGTTSTKKVVYASHSSVDSNVKDFINCFDSVSAV---VVSSPPIFDPFYVDILFSVAKGLPIFLSTSNVRIRSKVYVKYIMDITSSHILLQMTPTLYNSI-SFDLLKDTSKKFTFVLGGEQFPSL--SSIPPHSVYYNVFGVTEMSCWQSMVKIE---SILDIPILHVGSNLISNTDVYLVDDKSDRVVGDKIEGRIVVSSQIRRCF-----------RGESCVIYDAFETGDIGYYEKGYLYFNGRNHDSNIVKINGKRTNLLEMERRLKFILPSTLFHLDFYENALTLYCVNELENVVSSKILTMAXFSSFIMPSKIIKVGKIPFNANGKVN-KDQLRPSFHTKRKEAKNNILKEDVEDLWNKYTGILPKDTNNFILDGGDSHCSLAFLEEL---GVDPDI--FIEIVLNQSF---SDLVTALKGPIIMMVPSKRQMNVLEHKSKIV-----------ETQPSYRRRGFSTA-LSNVSSIKSINLA--WKIDLEKCIDASVLVV---DDCLAYVGSHSGIFLSCCVARGVVKWSIKLPDRIESSAIAS--SRFVIVGCYNHALYSIEKQSGAIAWSYSTSGLIKSSPIIIDELVIFGSYDYFVYSLHRDTGTLCWKTNFVNSXILTSP-AVTDGYGYVASLNGFALKLNTFSGEETWRVKLGTPIFSSGVTLRIGTTELFIFGGANGILYGINCDSSKIMWTFYAENSIFSSIALQGNTIAFGSHDHHLYIGYICETKFDLTAKIDLGHP--IYSTPYFFGPQKLAGISIDGRFVVVESKEVSLDVMLPV----NGQIFSSPIIFDKRLVFGCRDN 908          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. L. salmonis genes
Match: EMLSAG00000006022 (supercontig:LSalAtl2s:LSalAtl2s327:123273:124660:1 gene:EMLSAG00000006022 transcript:EMLSAT00000006022 description:"augustus_masked-LSalAtl2s327-processed-gene-1.0")

HSP 1 Score: 197.978 bits (502), Expect = 3.880e-55
Identity = 135/440 (30.68%), Postives = 225/440 (51.14%), Query Frame = 0

Query:  809 SQITVEPILFLFSLSHGLYMIIAQTLYIEKVCNVNLNFTREICDNIYVHPDEQIAVQKYVSELQAYNGIIQAIPSIIYALFAGPWSDRYGRRTLIAMACFGYIFNNGVFIINAYWFYELKAEYLLFECLQDCTGGSVCFFLGCYAYISDVSTRKNRTKRLAFLDGLFPIGFFTGMSISG-LIKKQWGFIPNFFLGMLAALLAVLYTVFVLKDSQDNRPEEVKAMLKEREAASTTTEPARKRNVCAQLFDLENLKASFRTTFKKRDNGMGS-ALTLLIFTFVVEIFMTHGRGATMFLYFRKEFAWTETQFGQFMALFGVVGLFTQYVAVPFLTDTCKIHDTLLGLGGVVGSGINLLIMALVRHDEWLYLGCVVAFLGPTITTSCRSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPGMFLIISACF 1246
            + ITVEP+   + +   +  ++   L+IEKVC V+LN++  IC N+  + + +  VQ+ V+EL+  +G + AIP+++++LF G WSDR GR+ L+ +   G I    V+I+N Y+F+EL    LL+       GG VC  +G    I+D ST  +RT RL++L+G+  + F  G +I G L      +   F + +   +L ++Y++F +K+S          ++ + E+  T             LFDL ++K  F+T  K R        + +L+  F   +F  +       L   K F W    F  F+ +  +V      + +P L+    I D  + L G + + ++ LI+AL +    +++  V  F    +T S RS  TK   P EVG VF+++   Q+ VPL + P FG +Y+ T+ TFPG +LII   F
Sbjct:    3 NYITVEPVQLFYGIMAMVSSVVRDHLFIEKVCQVHLNYSSNICSNLSQNKEIKDEVQEIVTELEIIDGTLVAIPAVVFSLFMGSWSDRNGRKFLLILPFIGNIIAFLVYILNYYFFFELGIYNLLWGSSVGMFGGYVCLNIGXXGXIADXSTITSRTTRLSYLNGITSLSFVIGSAIGGQLFNAMHNYYIIFGISIGFGILGIIYSIFFVKES----------IIPKEESEETNR----------GLFDLYHVKECFKTATKDRSTFYPRFGVIILVLHFASMMFCLNTSHYDYLLMVNK-FDWGVHDFSNFLIVTRIVRFVGLLLLLPLLSSVFHIRDEAITLFGTILTCLSYLIIALGQSSILMFVAVVFQF-NSIVTVSIRSQCTKATSPEEVGKVFAVIALGQSLVPLVSNPLFGLVYRETLTTFPGAYLIIVCAF 420          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. L. salmonis genes
Match: EMLSAG00000003464 (supercontig:LSalAtl2s:LSalAtl2s18:1125769:1129987:1 gene:EMLSAG00000003464 transcript:EMLSAT00000003464 description:"snap_masked-LSalAtl2s18-processed-gene-11.9")

HSP 1 Score: 105.145 bits (261), Expect = 3.795e-24
Identity = 71/287 (24.74%), Postives = 129/287 (44.95%), Query Frame = 0

Query:  952 YAYISDVSTRKNRTKRLAFLDGLFPIGFFTGMSISGLIKKQWGFIPNFFLGMLAALLAVLYTVFVLKDSQDNRPEEVKAMLKEREAASTTTEPARKRNVCAQLFDLENLKASFRTTFKKRDNGMGSALTLLIFTFVVEIFM-THGRGATMFLYFRKEFAWTETQFGQFMALFGVVGLFTQYVAVPFLTDTCKIHDTLLGLGGVVGSGINLLIMALVRHDEWLYLGCVVAFLGPTITTSCRSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPG 1237
            ++YI+D+S+ + R  R++ +DG   IG   G   SG++ K+ G++  F + +     + +Y +F + DS+ N       +  E E +S  T                 LK ++R  FKKR     S      F  ++ +F+     G  ++ Y    + W+E  +  F ++F  V     Y+  PF        D  LG+ G + S +  LI+    +     +G +   +G       RS ++K      +G +F+++G ++A VP+ +   F +IY  TVD +PG
Sbjct:   99 FSYITDISSVEQRNLRVSLIDGSLFIGLPLGSIFSGMMLKKIGYVGIFSISIAFMATSFIYVLFRINDSRSN-------LSVETEGSSGLTSI---------------LKETYRAVFKKRPERTAS------FLLMISMFLYVSCNGINVYYYTIVMYQWSEEMYSYFFSVFVFVDGIFSYIVYPFFMHKVHASDPTLGIVGELVSFVKSLILVYSFNTNIFIIGYIFGVIGSYTGVVIRSTISKISPKENIGKIFAVIGFLEALVPIVSSAVFTYIYNNTVDIYPG 357          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. L. salmonis genes
Match: EMLSAG00000001206 (supercontig:LSalAtl2s:LSalAtl2s11:1153615:1160710:-1 gene:EMLSAG00000001206 transcript:EMLSAT00000001206 description:"maker-LSalAtl2s11-augustus-gene-12.19")

HSP 1 Score: 72.0182 bits (175), Expect = 3.886e-13
Identity = 86/415 (20.72%), Postives = 163/415 (39.28%), Query Frame = 0

Query:  836 IEKVCNVNLNFTREICDNIYVHPDEQIAVQKYVSELQAYNGIIQAIPSIIYALFAGPWSDRYGRRTLIAMACFGYIFNNGVFIINAYWFYELKAEYLLFECLQ---DCTGGSVCFFLGCYAYISDVSTRKNRTKRLAFLDGLFPIGFFTGMSISGLIKKQWGFIPNFFLGML--AALLAVLYTVFVLKDSQDNRPEEVKAMLKEREAASTTTEPARKRNVCAQLFDLENLKASFRTTFKKRDNGMGSALTLLIFTFVVEIFMTHGRGATMFLYFRKEFAWTETQFGQFMALFGVVGLFTQYVAVPFLTDTCKIHDTLLGLGGVVGSGINLLIMALVRHDEWLYL-GCVVAFLGPTITTSCRSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPGMFLIISA 1244
            I K C ++LN+T ++CDN+    +E             YN  +Q   S+          D  GR+T++ +     + +  + I N Y+  E  + Y+    +       GG    F+   A+I+D+S  +    RL  L     I       +   +    GF+  +  G +  AA    LY                  M+K +    TT    RK++   +L         F+T  K R   +   L L +   ++ +    G     +LY R  + W  T F  + ++  V  +F Q   +P L+    I++    +  ++   +  L M L  ++ WLY  G ++  +G    +  R+ ++  +   E+G V S++ +++  + + +   +  I+  T    PG    +SA
Sbjct:    3 IYKACRIDLNYTSQLCDNLLDDGNED------------YNKKVQDTVSLFMV-------DAIGRKTILNVHALATVLSQALLIANGYFLVEW-SNYIYILTVSVPIMLVGGKSTSFIATTAFITDISPPRMAXVRLGILHVTQSISEPLANIVGAFLLDSGGFVVVYLFGFIFSAAAYVSLYV----------------CMMKYK----TTITEKRKKSF-RELMSPRIFIDCFKTFSKVRAGKLRMILYLSLVNLILILTSIIGEATIAYLYVRTRYKWEXTDFSFYKSIDKVTAIFFQSALIPLLS-YVNINNENFLICIIISFCLRHLTMGLA-YEPWLYYAGTMLDVIGSYAFSIIRATISDILPKSEMGKVLSMVISLENIMIIISIKIYQTIWSMTDTILPGTVYFVSA 374          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. SwissProt
Match: gi|5915761|sp|O68007.1|BACB_BACLI (RecName: Full=Bacitracin synthase 2; Short=BA2; Includes: RecName: Full=ATP-dependent lysine adenylase; Short=LysA; AltName: Full=Lysine activase; Includes: RecName: Full=ATP-dependent D-ornithine adenylase; Short=D-OrnA; AltName: Full=D-ornithine activase; Includes: RecName: Full=Ornithine racemase)

HSP 1 Score: 84.3445 bits (207), Expect = 5.076e-15
Identity = 109/435 (25.06%), Postives = 187/435 (42.99%), Query Frame = 0

Query:    5 ADVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSL-FELVKSGPAFGAQ-STLKYVVLGGEPFP----KAIPSPAPAHLRYFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSDT-----HVSLTSQ-----GEIVVSSATRICRVHPGQGTQHTEK---------GYSVFTGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVL-KEDRRVLGF-----------HSQDIDPSRLSHLPPILRGVELVKVPRFPITRHGKIDLKLLLEWK--------EETHRKKVGLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFINEIEDATNNPSVQSLL 393
             D+AYVI TSGTTG  K V V   +I      + + + LAS + ++L L+  +FD F  ++  ++L    ++ V   +KL   +   +I ++ I  ++ TPSL   L+K         S++K +++G +       K +       +R  N+YGVTE S      E        P    T  G  L +T       SL  Q     GE+ +     I R +  +     E+         G    TGDL  A+   DG   + F  R  + VK++G RI   E+   L +        V+ +ED++   +           ++ ++      HLP  +     +++ + P+T +GKID K L E           E  R +    +A +W+++   D + ++ NF   GGDSI A++ ++++  A     V+ L 
Sbjct: 1690 GDLAYVIYTSGTTGNPKGVMVEHRNIIHAHYTWRKHYELASFSVNLLQLASMSFDVFAGDLCRSLLNGGTMYIVPDDVKLEMNLLYDMINKYGIHMLESTPSLIIPLMKYIDHHKLDFSSMKLLIMGSDTCTIKDYKWLVERFGQRMRIINSYGVTEASVDSGYYE--EALDRIPEIANTPIGKPLDNTAFYILDPSLNPQPVGVYGELYIGGEG-IARGYLNKPELTKERFVPNRFAAGGNMYKTGDL--ARWLPDGN--VEFLGRIDHQVKIRGFRIETGEIETKLLENQNISEAVVIDREDKKGHKYLCAYIVARAKTNTNELREYLSDHLPDYMLPSYFIQINKMPLTPNGKIDRKALPEPAGDVIAASGYEAPRNETEEKLAAVWQEVLDRDKIGINDNFFEIGGDSIKALQIVSKLSRADLKLQVKDLF 2117          

HSP 2 Score: 67.3958 bits (163), Expect = 7.041e-10
Identity = 100/424 (23.58%), Postives = 179/424 (42.22%), Query Frame = 0

Query:    5 ADVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRI-KLNAVMFSQVIQRHRIAFIQLTPSLFELVKSGPAFGAQSTLKYVVLGGEPFPKAIPSPAPAHLRYFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSDTHVSLTSQ----------GEIVVSSATRICRVHPGQGTQHT------EKGYSVF-TGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQ------DLSQALGCPFHCVLKEDRR-------VLGFHSQDIDPSRLSH-----LPPILRGVELVKVPRFPITRHGKIDLKLLLE--------WKEETHRKKVGLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFINEIEDA 383
            +D+AYVI TSG++GR K V  +  ++   V  F +  PL S  D+VL +   +FD F  E+   +LA      +S ++  LN     + I ++R+  +  +P L   +           +K++  GG+                +N+YG TE +   +  ++   SS+         G  LS+  V +  Q          GE+++     + R +    T         E G  V+ TGDL  A+   DG   I F  R  + VK++G RI   E+        ++++A+      V KED+         + F++++ D  ++       LP  +     +K+ + P T +GK DLK L E         + E  R +    +  +W+ +   + + ++ +F   GG S+ A     +I+ A
Sbjct:  657 SDLAYVIYTSGSSGRPKGVMTTHRNVVHYVDAFTKRIPL-SEHDTVLQVVSFSFDAFSEEV-YPILACSGRLVISRKVSDLNIDELVKTIGKYRVTLVSCSPLLLNEIDKNQHLTFHPQMKFIS-GGDVLKFEYVENIIKGADVYNSYGPTEATVCATYYQL---SSADRKKTSIPIGKPLSNYKVYIADQYGRPQPVGVPGELLIGGEG-VARGYLNHETLTKAAFVVDESGERVYRTGDL--ARWLSDGN--IEFLGRIDSQVKIRGYRIELEEIEHRLLMNDNINEAI-----VVAKEDQENSKYLCAYIAFNNKNADIEQVQERLAKDLPEYMIPSCFIKLDQIPRTINGKADLKALPEPDRRAFAQARYEAPRNQTEALLLSIWQDILPAEQIGINDHFFDIGGHSLKAFSMAAKIQSA 1064          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. SwissProt
Match: gi|81906124|sp|Q9DC26.1|S46A3_MOUSE (RecName: Full=Solute carrier family 46 member 3; Flags: Precursor)

HSP 1 Score: 80.8777 bits (198), Expect = 1.756e-14
Identity = 93/389 (23.91%), Postives = 164/389 (42.16%), Query Frame = 0

Query:  864 VQKYVSELQAYNGIIQAIPSIIYALFAGPWSDRYGRRTLIAMACFGYIFNNGVFIINAYWFYELKAEYLLFECLQDCTGGSVCFFLG-CYAYISDVSTR-KNRTKRLAFLDGLFPIGFFTGMS--ISGLIKKQWGFIPNFFLGMLAALLAVLYTVFVLKDSQDNRPEEVKAMLKEREAASTTTEPARKRNVCAQLFDLENLKASFRTTFKKRDNGMG---SALTLLIFTFVVEIFMTHGRGATMFLY-FRKEFAWTETQFGQFMALFGVVGLFTQYVAVPFLTDTCKIHDTLLGLGGVVGSGINLLIMALVRHDEWLYLGCVVAFLGPTITTSCRSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPGMFLIISA 1244
            VQK  S       +   IP ++        SD +GR+  + ++  G +  N    + +Y  ++L  + L+         G+   F G C+AYI D     K+R  R+A LD  F +G  TG++   SG   ++ GF+ ++F+  +  ++ + Y +F L D       ++  M                   C     +E+LK  F  T+    NG     + L LLIFT V+  F+  G      LY       W E   G   AL G V   + ++ + +L   C + D  +   G+  + + + + A  R    ++L  +         +  RS+++K V   E GA+F+ +  ++    + +   +  IY ATV  +PG   ++SA
Sbjct:   65 VQKKASLFNLQVEMSALIPGLVSTFMLLASSDNHGRKLPMVLSSLGSLGTNTWLCMMSY--FDLPLQLLIASTFIGALFGNYTTFWGACFAYIVDQQKEYKHRIIRIAILD--FMLGVVTGLTGLSSGYFIRELGFVWSYFITAMVLIVNLAYILFFLNDPIKESSSQIVTM------------------SC-----IESLKDLFYRTYMLFKNGSSKRQALLCLLIFTLVIYFFVIIGISPIFTLYELGPPLCWNEVYIGYGSAL-GSVSFLSSFLGI-WLFSYC-LKDIHIAYIGIFTTMVGMTLAAFTRTTLMMFLVRIPFIFTIMPLSVLRSMLSKVVHSTEQGALFACIAFLETLAGVTSTSAYSGIYSATVAWYPGFIFLLSA 423          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. SwissProt
Match: gi|81882604|sp|Q5BK75.1|S46A3_RAT (RecName: Full=Solute carrier family 46 member 3; Flags: Precursor)

HSP 1 Score: 79.337 bits (194), Expect = 5.462e-14
Identity = 107/449 (23.83%), Postives = 187/449 (41.65%), Query Frame = 0

Query:  813 VEPILFLFSLSHGLYM-IIAQTLYIEKVCNVNLNFTREICDNIYVHPDE--------QIAVQKYVSELQAYNGIIQAIPSIIYALFAGPWSDRYGRRTLIAMACFGYIFNNGVFIINAYWFYELKAEYLLFECLQDCTGGSVCFFLG-CYAYISDVSTR-KNRTKRLAFLDGLFPIGFFTGMS--ISGLIKKQWGFIPNFFLGMLAALLAVLYTVFVLKDSQDNRPEEVKAMLKEREAASTTTEPARKRNVCAQLFDLENLKASFRTTFKKRDNG---MGSALTLLIFTFVVEIFMTHGRGATMFLY-FRKEFAWTETQFGQFMALFGVVGLFTQYVAVPFLTDTCKIHDTLLGLGGVVGSGINLLIMALVRHDEWLYLGCVVAFLGPTITTSCRSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPGMFLIISA 1244
            +EP + L++ +  L + + AQ +Y  ++     N+T     N+              Q  VQK  S       I   IP ++        SD +GR+  + ++  G +  N    + A  +++L  + L+         G+   F G C+AYI D     K+R  R+A LD  F +G  TG++   SG   ++ GF  ++F+  +  L+ + Y +F L D       ++  M                   C+     E+LK  F  T+    NG     S L LLIFT VV  F+  G      LY       W E   G   AL G +   + ++ + +L   C + D  +   G+  + + +++ A  R    ++L  +  F      +  RS+++K V   E G +F+ +  ++    + +   +  IY ATV  +PG   ++SA
Sbjct:    6 IEPAILLYAFAMTLTIPLTAQYVY-RRIWEETGNYTFTSSSNVSECEQNKSSSTFAFQEEVQKKASLFSLQVEISGLIPGLVSTFMLLSSSDNHGRKLPMVLSSLGSLGTN--LWLCAMSYFDLPLQLLVASTFIGALFGNYTTFWGACFAYIVDQEKEYKHRIIRIAVLD--FMLGVVTGLTGLSSGYFIRELGFAWSYFIIAVVVLVNLAYILFFLSDPIKESSSQIVTM------------------SCS-----ESLKDLFYRTYMLFKNGSCKRRSLLCLLIFTLVVYFFVVFGITPVFTLYELGPPLCWNEVYIGYGSAL-GSLSFLSSFLGI-WLFSYC-LKDIHIAYVGIFTTMVGMMLTAFTRTTLMMFLVRISFFFTIMPLSILRSMLSKVVHSTEQGVLFACIAFLETLGGVTSTSAYNGIYSATVAWYPGFVFLLSA 423          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. SwissProt
Match: gi|1239396319|sp|E9F8M3.1|SWNK_METRA (RecName: Full=Reducing polyketide synthase swnK; AltName: Full=Swainsonine biosynthesis gene cluster protein K)

HSP 1 Score: 79.7221 bits (195), Expect = 1.459e-13
Identity = 97/424 (22.88%), Postives = 180/424 (42.45%), Query Frame = 0

Query:    1 DLERADVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFE-LVKSGPAFGAQSTLKYVVLGGEPFPKAIPSP-APAHLRYFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSD--THVSLTSQGEIVVSSAT---------RICR----VHPGQGTQHTEKGYSVFTGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVLKEDRRVLGF---HSQDIDPSRL---------SHLPPILRGVELVKVPRFPITRHGKIDLKLLLEWKEETHRK-------KVGLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFINEIEDATNNP 387
            +++  D+AYVI TSG+TGR K V +S  +    +    +  P  S  D +L+++  +FD   LE+LL +++   +   +     +      ++ RH++  +Q TP+ +  L++SG  +     L  V+ GGEP  + +      A    +N YG +E + + S+  V  G      P+      +L D  + V + S+GE+ +   +          + R    V+P  G      G    TGDL  A+    G+  ++   R    VK++G RI   ++   L          V+  D R++ +   H+   D + L           LP  +     V++   P++   K++ K L +  E    +       ++   +  +W  + G D   ++ NF R GGDS+  +     +E   + P
Sbjct:  165 EIQTRDLAYVIYTSGSTGRPKGVEISHGAAANFLSSLRKHEPGCSERDRLLAITTISFDMSALELLLPLVSGSAMIVANTSAVKDPRELISLMARHKVTILQATPATWTMLLESG--WKGNPRLSKVICGGEPLSRQLADRLLAAADSVWNVYGPSE-TTYGSVGRVGQGDIVVGNPVANGRIYVLDDNMSPVPIGSEGEVYIGGGSVSNGYRNKAELTRSRFLVNPFHG------GVFFRTGDL--ARFIAPGKLQVV--GRIDGVVKIRGHRIDVGDIEAVLVDHANVSEAVVVSRDDRLVAYCVLHAPCHDAASLDGILRPWVAERLPAYMLPTFFVQMDALPLSPSEKVNRKALPDPIEAMQHQTMIQPTSELEQRIQAIWSDILGHDRFGIEDNFFRIGGDSVRIIRMQAALEKQLHRP 575          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. SwissProt
Match: gi|6136084|sp|O30409.1|TYCC_BREPA (RecName: Full=Tyrocidine synthase 3; AltName: Full=Tyrocidine synthase III; Includes: RecName: Full=ATP-dependent asparagine adenylase; Short=AsnA; AltName: Full=Asparagine activase; Includes: RecName: Full=ATP-dependent glutamine adenylase; Short=GlnA; AltName: Full=Glutamine activase; Includes: RecName: Full=ATP-dependent tyrosine adenylase; Short=TyrA; AltName: Full=Tyrosine activase; Includes: RecName: Full=ATP-dependent valine adenylase; Short=ValA; AltName: Full=Valine activase; Includes: RecName: Full=ATP-dependent ornithine adenylase; Short=OrnA; AltName: Full=Ornithine activase; Includes: RecName: Full=ATP-dependent leucine adenylase; Short=LeuA; AltName: Full=Leucine activase)

HSP 1 Score: 79.7221 bits (195), Expect = 1.483e-13
Identity = 109/425 (25.65%), Postives = 182/425 (42.82%), Query Frame = 0

Query:    4 RADVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFD----PFYLEILLA---VLA----HRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFELVKSGPAFGAQSTLKYVVLGGEPFPKAIPSP--APAHLRYFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSDTHVSLTSQGEIVVSSATRICRVHPG------QGTQHT---------EKGYSVF-TGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVL-KEDRR------VLGFHSQDIDPSRL-----SHLPPILRGVELVKVPRFPITRHGKIDLKLLLEWKEETH--------RKKVGLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFIN 378
            + D+AYVI TSG+TG  K V +S  +I  ++      FPL +  D+VL  +P +FD     FYL ++     VLA    HR I +++           ++I+  +I  +Q+ PSL +LV + P + A ++L+ V  GGE    A+ S        +  N YG TE +   +             PI     N+    +V   S     V  A  +C    G      Q  + T         E G +++ TGD+   +   DG   I +  R  + VK++G RI   E+   L Q        V+ ++D +            QDI  + L     + LP  +     V + + P++ +GK+D K L + ++           R +    +A +W  + G++P+ V  +F   GG S+ A+  I+
Sbjct: 1639 KDDLAYVIYTSGSTGNPKGVMISHQAICNHMLWMRETFPL-TTEDAVLQKTPFSFDASVWEFYLPLITGGQLVLAKPDGHRDIAYMT-----------RLIRDEKITTLQMVPSLLDLVMTDPGWSACTSLQRVFCGGEALTPALVSRFYETQQAQLINLYGPTETTIDATYWPCPRQQEYSAIPIGKPIDNV--RLYVVNASNQLQPVGVAGELCIAGDGLARGYWQREELTKASFVDNPFEPGGTMYRTGDM--VRYLPDGH--IEYLGRIDHQVKIRGHRIELGEIEATLLQHEAVKAVVVMARQDGKGQNSLYAYVVAEQDIQTAELRTYLSATLPAYMVPSAFVFLEQLPLSANGKVDRKALPQPEDAAASAAVYVAPRNEWEAKLAAIWESVLGVEPIGVHDHFFELGGHSLKAMHVIS 2045          

HSP 2 Score: 76.6406 bits (187), Expect = 1.269e-12
Identity = 107/416 (25.72%), Postives = 186/416 (44.71%), Query Frame = 0

Query:    5 ADVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLF-ELVKSGPAFGAQSTLKYVVLGGEPF-PKAIPSPAPA--HLRYFNAYGVTEMSCWQS--LIEVQPGSSSCP--PPIWTSTGNLL--SDTHVSLTSQGEIVVSSATRICRVHPGQGTQHTEK--------GYSVF-TGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQ--ALGCPFHCVLKE--DRRVLGFH---SQDIDPSRL-----SHLPPILRGVELVKVPRFPITRHGKIDLKLLLEWKEE--------THRKKVGLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFINEI 380
            +D+AYV+ TSG+TG+ K V V   ++   V   H  +     +D ++      FD    EI  A+L    ++ VS  + L+A      ++ ++I  + LT  LF +L +  PA     +L+ +++GGE   PK I     A   L  +N YG TE + + +  LIE Q      P   PI  ST  ++  ++    +   GE+ V     + R +  +     EK        G +++ TGDL  A+   DG   I +  R    VK++G RI   E+   LSQ   +      V++E   ++ L  +    Q +D + L       LP  +     V++ + P+T +GK+D + L +   E          +      + ++W+++ GI  + +  NF   GG S+ A+  I ++
Sbjct: 3715 SDLAYVMYTSGSTGKPKGVMVEHRNVTRLV--MHTNYVQVRESDRMIQTGAIGFDAMTFEIFGALLHGASLYLVSKDVLLDAEKLGDFLRTNQITTMWLTSPLFNQLSQDNPAMF--DSLRALIVGGEALSPKHINRVKSALPDLEIWNGYGPTENTTFSTCYLIE-QHFEEQIPIGKPIANSTAYIVDGNNQPQPIGVPGELCV-GGDGVARGYVNKPELTAEKFVPNPFAPGETMYRTGDL--ARWLPDG--TIEYLGRIDQQVKIRGYRIELGEIETVLSQQAQVKEAVVAVIEEANGQKALCAYFVPEQAVDAAELREAMSKQLPGYMVPAYYVQMEKLPLTANGKVDRRALPQPSGERTTGSAFVAAQNDTEAKLQQIWQEVLGIPAIGIHDNFFEIGGHSLKAMNVITQV 4120          

HSP 3 Score: 67.781 bits (164), Expect = 7.355e-10
Identity = 104/427 (24.36%), Postives = 183/427 (42.86%), Query Frame = 0

Query:    6 DVAYVIQTSGTTGRRKSVCV---SWASIEANVRDFHRL--FPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFELVKSGPAFGA--QSTLKYVVLGGEPFP----KAIPSPAPAHLRYFNAYGVTEMSCWQSLIE------VQPGSSSCPPPIWTSTGNLLSDTHVSLTS---QGEIVVSSATRICRVHPGQGTQHTEK--------GYSVF-TGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVL-KEDR--------RVLGFHSQDIDPSR---LSHLPPILRGVELVKVPRFPITRHGKIDLKLL-------LEWKEETHRKKV-GLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFINEIED 382
            +VAY+I TSGTTG+ K V +   S+A++    +D + L  FP+      +L ++   FD    +   A+L    +    + +K++     + I+RH I   + TP+L   +           S +K ++LG +  P    K + +     +R  N+YGVTE     S  E      ++ G+     P+   T  ++ D H++L      GE+ +  A  + R +  +     EK        G  ++ TGDL + +  G+    + F  R  + VK++G RI   E+   L +  G     V+ +EDR         V+  H  D    R   L  LP  +     V +   P+T +GK+D + L       L   E    + V    +A++W Q+  +  V    +F   GG S+ A+  ++ + +
Sbjct: 4751 NVAYIIYTSGTTGKPKGVVIEHRSYANVAFAWKDEYHLDSFPV-----RLLQMASFAFDVSTGDFARALLTGGQLVICPNGVKMDPASLYETIRRHEITIFEATPALIMPLMHYVYENELDMSQMKLLILGADSCPAEDFKTLLARFGQKMRIINSYGVTEACIDTSYYEETDVTAIRSGTVPIGKPLPNMTMYVV-DAHLNLQPVGVVGELCIGGAG-VARGYLNRPELTEEKFVPNPFAPGERLYRTGDLAKWRADGN----VEFLGRNDHQVKIRGVRIELGEIETQLRKLDGITEAVVVAREDRGQEKELCAYVVADHKLDTAELRANLLKELPQAMIPAYFVTLDALPLTANGKVDRRSLPAPDVTMLRTTEYVAPRSVWEARLAQVWEQVLNVPQVGALDDFFALGGHSLRAMRVLSSMHN 5166          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. SwissProt
Match: gi|239938827|sp|Q04747.3|SRFAB_BACSU (RecName: Full=Surfactin synthase subunit 2)

HSP 1 Score: 77.7962 bits (190), Expect = 4.932e-13
Identity = 108/440 (24.55%), Postives = 173/440 (39.32%), Query Frame = 0

Query:    6 DVAYVIQTSGTTGRRKSV---------CVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFELVKSGPAFGAQS---TLKYVVLGGEPFPKAIPSPAPA------HLRYFNAYGVTEMSCWQSLIEV---QPGSSSCPPPIWTSTGNLLSDTHVSLTSQGEIVVSSATRICRVHPGQGTQHTEKGYSVFTGDLGEAQCTGD-----------------------GQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVLKED----RRVLGFHS--QDIDPSRLSH-----LPPILRGVELVKVPRFPITRHGKIDLKLLLEWKEETHRKK--------VGLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFINEIE 381
            ++AYVI TSGTTG+ K V          VSW S EA + +           D  + LS   FD  Y  +   +L    +H V           +  I+ H I +I+LTPSLF  + +  +F   +   +L+ +VLGGE   K IP+   A      H  + N YG TE +       V   +P + +  P I     N            G +V++ A ++  V PG   Q    G  +  G L   Q T +                           + F  R  + VK++G RI   E+   +    G     VL       + +  +++  QDI+ + L +     LP  +     V+V   P+T +GK D   L +        K        +  ++ ++W++  GI+ + +D NF   GG S+  +  I  I+
Sbjct: 1638 NLAYVIYTSGTTGKPKGVQIEHRNLTNYVSWFSEEAGLTE----------NDKTVLLSSYAFDLGYTSMFPVLLGGGELHIVQKETYTAPDEIAHYIKEHGITYIKLTPSLFHTIVNTASFAKDANFESLRLIVLGGE---KIIPTDVIAFRKMYGHTEFINHYGPTEATIGAIAGRVDLYEPDAFAKRPTIGRPIAN-----------AGALVLNEALKL--VPPGASGQLYITGQGLARGYLNRPQLTAERFVENPYSPGSLMYKTGDVVRRLSDGTLAFIGRADDQVKIRGYRIEPKEIETVMLSLSGIQEAVVLAVSEGGLQELCAYYTSDQDIEKAELRYQLSLTLPSHMIPAFFVQVDAIPLTANGKTDRNALPKPNAAQSGGKALAAPETALEESLCRIWQKTLGIEAIGIDDNFFDLGGHSLKGMMLIANIQ 2051          

HSP 2 Score: 76.6406 bits (187), Expect = 1.390e-12
Identity = 104/415 (25.06%), Postives = 183/415 (44.10%), Query Frame = 0

Query:    5 ADVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFELVKSGPAFGAQSTLKYVVLGGEPFPKAIPSP---APAHLRYFNAYGVTEMSCWQS--LIEVQPGSS-SCPPPIWTSTGNLLSDTH--VSLTSQGEIVVSSATRICRVHPGQGTQHTEK---------GYSVF-TGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVL-----KEDRRVLGFHSQD--IDPSRL-----SHLPPILRGVELVKVPRFPITRHGKIDLKLLLEWKEETHRKKVGL-------NVAKLWRQLTGIDPVVDSNFVRDGGDSILAVEFINEIED 382
            +D+AY++ TSG+TG+ K V +   SI   V++    +   +  D +      +FD    E+  A+L    ++ V     L+A  F+  ++   I  + LT  LF  + +  A G   TL+++++GG+     I S    A   L  +N YG TE + + +  LI+ + G S     PI  ST  ++ +      + + GE+ V     + R +     + TEK         G  ++ TGDL  A+   DG   I F  R  N VK++G RI   E+   L+ A       V+      ++  +  + + D  +  S L       LP  +    L+++   P+T +GKI+ K L   + E  + +           +A++W  + G+   V  NF   GG S+ A+    +I++
Sbjct:  606 SDLAYIMYTSGSTGKPKGVMIEHKSILRLVKNAG--YVPVTEEDRMAQTGAVSFDAGTFEVFGALLNGAALYPVKKETLLDAKQFAAFLREQSITTMWLTSPLFNQLAAKDA-GMFGTLRHLIIGGDALVPHIVSKVKQASPSLSLWNGYGPTENTTFSTSFLIDREYGGSIPIGKPIGNSTAYIMDEQQCLQPIGAPGELCV-GGIGVARGYVNL-PELTEKQFLEDPFRPGERIYRTGDL--ARWLPDGN--IEFLGRIDNQVKVRGFRIELGEIETKLNMAEHVTEAAVIIRKNKADENEICAYFTADREVAVSELRKTLSQSLPDYMVPAHLIQMDSLPLTPNGKINKKELPAPQSEAVQPEYAAPKTESEKKLAEIWEGILGVKAGVTDNFFMIGGHSLKAMMMTAKIQE 1011          

HSP 3 Score: 57.7658 bits (138), Expect = 6.780e-7
Identity = 104/456 (22.81%), Postives = 180/456 (39.47%), Query Frame = 0

Query:    6 DVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRH--RIAFI--QLTPSLFELVKSGPAFGAQSTLKYVVLGGEPFPKAIPSPAPAHLRYFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSDTHVSLTSQGEIVVSS--ATRICRVHPGQGT---QHTEKGYSVFTGD---LGEAQC-TGDGQQ----AILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVLKEDRRVLGFHSQDIDPSRLSHLPPILRGVELVK-----------VPRF-------PITRHGKIDLKLLL---------EWKEETHRKKVGLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFIN---------EIEDATNNPSVQSLLELLLNGQFGEFQDQ 407
            ++AY+I TSGTTG+ K V V   ++  N+  +H      +  D     +   FD    E+         +H +   I+L+ V  +   + +   I F+  QL     EL          ++L+ ++ GG+   +A+  P        N YG TE +   +  E+ P   S       S G  +++T V +  +G  V     A  +C    G         ++    F  D    GE    TGD  +     I +  R    VK++G RI   E+   L+Q           +D  V     +  + +  +++ P    +E +K           +P F       P+T +GK+D K L          E+K  T   +  L  A +W+ + G+  V V  NF   GGDSI  ++  +         E++D   +P+++ L + +   + G+  DQ
Sbjct: 2688 NLAYMIYTSGTTGQPKGVMVEHHAL-VNLCFWHHDAFSMTAEDRSAKYAGFGFDASIWEMFPTWTIGAELHVIDEAIRLDIVRLNDYFETNGVTITFLPTQLAEQFMEL--------ENTSLRVLLTGGDKLKRAVKKP----YTLVNNYGPTENTVVATSAEIHPEEGSL------SIGRAIANTRVYILGEGNQVQPEGVAGELCVAGRGLARGYLNREDETAKRFVADPFVPGERMYRTGDLVKWVNGGIEYIGRIDQQVKVRGYRIELSEIEVQLAQLSEV-------QDAAVTAVKDKGGNTAIAAYVTPETADIEALKSTLKETLPDYMIPAFWVTLNELPVTANGKVDRKALPEPDIEAGSGEYKAPTTDMEELL--AGIWQDVLGMSEVGVTDNFFSLGGDSIKGIQMASRLNQHGWKLEMKDLFQHPTIEELTQYVERAE-GKQADQ 3114          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. SwissProt
Match: gi|81669034|sp|O31827.1|PPSE_BACSU (RecName: Full=Plipastatin synthase subunit E; AltName: Full=Peptide synthase 5; Includes: RecName: Full=ATP-dependent isoleucine adenylase; Short=IsoA; AltName: Full=Isoleucine activase)

HSP 1 Score: 76.6406 bits (187), Expect = 1.145e-12
Identity = 116/470 (24.68%), Postives = 195/470 (41.49%), Query Frame = 0

Query:    6 DVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFELVKSGPA--FGAQSTLKYVVLGGEPFPKAIPSP---APAHLRYFNAYGVTEMSCWQSLIEVQPGSSSCPP---PIWTSTGNLLSDTHVSLTSQGEIVVSSATRICRVHPGQ---GTQHTEKGY--------SVF-------------TGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVLKEDR--------RVLGFHSQ---DIDPSRLSHLPPILRGVELVKVPRFPITRHGKIDLKLL-LEWKEETHRKKVGL-------NVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFINEIEDATN----------NPSVQSLLELLLNGQFGEFQDQIMARIG 413
            D+AY++ TSG+TGR K V ++  ++ + VR+ +  +  AS  D  +     +FD    E+  A+L    +H +     L    F   +  + I  + LT +LF  +    A  F    TL YV  GGE    A+ +    A   L   N YG TE + + +  E++   +   P   PI  ST  +L DT   L   G              PG+   G     KGY        +VF             TGDL  A+   DG   + + +R    +K++G+RI   E+   L +  G     V   ++          +G H +   D        LP  +     V+V R P+T +GKID   L +   +   R+ + L        +A +W+Q+ G++ + +D +F   GG S+ A++ I+ ++   N          +P++  L E L + Q     +Q + ++ 
Sbjct:  617 DLAYIMYTSGSTGRPKGVMITNRNVVSLVRNSN--YTSASGDDRFIMTGSISFDAVTFEMFGALLNGASLHIIDKSTMLTPDRFGAYLLENDITVLFLTTALFNQLAQVRADMFRGLHTL-YV--GGEALSPALMNAVRHACPDLALHNIYGPTENTTFSTFFEMKRDYAGPIPIGKPISNSTAYIL-DTKGRLLPIGV-------------PGELCVGGDGVAKGYLNRVDLTNAVFSPHPFLPGERIYRTGDL--ARWLPDGN--LEYISRIDRQMKIRGKRIEPAEIEARLLEMEGVQEAAVTLREKDGEAQLYTHYVGDHKKTDTDFRADLARVLPDYMIPQHWVRVERMPLTGNGKIDRSALPIPENKPAKRQNIILPRNLVEEELANIWKQVLGVNTISIDDDFFAIGGHSLRALQVIHTLKHQQNIDIPIDFLFEHPTIAQLAEKLYSKQLTAANEQHVIKLN 1063          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. SwissProt
Match: gi|730367|sp|P39846.1|PPSB_BACSU (RecName: Full=Plipastatin synthase subunit B; AltName: Full=Peptide synthase 2; Includes: RecName: Full=ATP-dependent tyrosine adenylase 1; Short=TyrA 1; AltName: Full=Tyrosine activase 1; Includes: RecName: Full=ATP-dependent threonine adenylase; Short=ThrA; AltName: Full=Threonine activase)

HSP 1 Score: 76.6406 bits (187), Expect = 1.157e-12
Identity = 97/433 (22.40%), Postives = 185/433 (42.73%), Query Frame = 0

Query:    8 AYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSL-SPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFE-LVKSGPAFGAQSTLKYVVLGGEPF-PKAIPS--PAPAHLRYFNAYGVTEMSCW-----QSLIEVQPGSSSCPPPIWTSTGNLLSDTHVSLTSQG---EIVVSSATRICRVHPGQGTQHTEK--------GYSVF-TGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDL----------------SQALGCPFHCVLKEDRRVLGFHSQDIDPSRLSHLPPILRGVELVKVPRFPITRHGKIDLKLLLEWKEETH-------RKKVGLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFINEIEDATNNPSVQSLLE 394
            AYVI TSGTTG+ K V V   ++ + ++  H+  P     + V +L     FD    E+  A+L    +  VS     +   F  ++++ R+  +  TP+ F  L+           ++YV+ GGE   P  + S      H    N YG+TE +        S  ++    S+   P+ T   +++ D H++L   G   E+ +     + R +  +     ++        G  ++ TGDL +    G+    + +  R    VK++G RI   E+   L                 Q     +  ++ +D++       DI     + LP  +    ++++   P+T +GK+D K L E +++ +       R ++   +A++W +L  +D + V +NF + GGDSI A++    ++      +V+ + E
Sbjct: 1643 AYVIYTSGTTGQPKGVIVEHRNVISLLK--HQNLPFEFNHEDVWTLFHSYCFDFSVWEMFGALLNGSTLVVVSKETARDPQAFRLLLKKERVTVLNQTPTAFYGLMLEDQNHTDHLNIRYVIFGGEALQPGLLQSWNEKYPHTDLINMYGITETTVHVTFKKLSAADIAKNKSNIGRPLSTLQAHVM-DAHMNLQPTGVPGELYIGGEG-VARGYLNRDELTADRFVSNPYLPGDRLYRTGDLAKRLSNGE----LEYLGRIDEQVKVRGHRIELGEIQAALLQYPMIKEAAVITRADEQGQTAIYAYMVIKDQQAANI--SDIRTYLKNALPDFMLPARMIQIDSIPVTVNGKLDQKALPEPEKQAYTADDISPRNEIETVMAEIWEELLNVDELGVSANFFKLGGDSIKALQVCARLKQRGFETTVREMFE 2065          

HSP 2 Score: 62.003 bits (149), Expect = 3.366e-8
Identity = 96/420 (22.86%), Postives = 168/420 (40.00%), Query Frame = 0

Query:    6 DVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFELVKSGPAFGAQSTLKYVVLGGEPFPKAI--------PSPAPAHLRYFNAYGVTEMSCWQSLIE--VQPGSSSCPPPIWTSTGNLLSDTHVS-LTSQGEIVVSSATRICRVHPGQGTQHTEKGYS----------VFTGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVL-----KEDRRVLGF---HSQDIDPSRLSH----LPPILRGVELVKVPRFPITRHGKIDLKLLLEWKEETH------RKKVGLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVEFINEIEDATN 385
            D+AY+I TSGTTGR K V V    I AN   + R     +  D  L L    FD     +   +L+   +   +     + +   + I R++++ + + PSL+ ++          +L+ V   GE     +        PS   A     N YG TE S   +++    +    +   PI  +   +L    +  + + GE+ +S A  + R +  Q  + T+K +S            TGD G     G     I +  R  + VK++G RI   E+   L QA G     VL      E++ ++ +      +  P    H    LP  +    ++ + + P+T  GK+D   L E +  T       R  +  ++A +W  +     + +  +F + GG S+ A   ++ I    N
Sbjct:  608 DLAYLIYTSGTTGRPKGVLVDHHGI-ANTLQWRREEYSMTEQDISLHLFSYVFDGCVTSLFTPLLSGACVLLTTDDEAKDVLALKRKIARYKVSHMIIVPSLYRVLLEVMTADDAKSLRIVTFAGEAVTPDLLELNQIICPSAELA-----NEYGPTENSVATTILRHLNKKERITIGHPIRNTKVFVLHGNQMQPIGAAGELCISGAG-LARGYYKQ-QELTQKAFSDHPFLEGERLYRTGDAGRFLPDG----TIEYIGRFDDQVKIRGYRIELREIETVLRQAPGVKEAAVLARDVSAEEKELVAYIVPEKGNSLPDLYQHLAGTLPSYMIPASIINISQMPLTSSGKLDRFALPEPENNTSVTYMAPRTLIEADLAHIWEDVLNKQHIGIRDDFFQLGGQSLKAAALVSRIHKKLN 1015          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. SwissProt
Match: gi|6093777|sp|P39847.2|PPSC_BACSU (RecName: Full=Plipastatin synthase subunit C; AltName: Full=Peptide synthase 3; Includes: RecName: Full=ATP-dependent glutamate adenylase 2; Short=GluA 2; AltName: Full=Glutamate activase 2; Includes: RecName: Full=ATP-dependent alanine/valine adenylase; Short=Ala/ValA; AltName: Full=Alanine/valine activase)

HSP 1 Score: 75.485 bits (184), Expect = 2.472e-12
Identity = 101/414 (24.40%), Postives = 186/414 (44.93%), Query Frame = 0

Query:    7 VAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFELV--KSGPAFGAQSTLKYVVLGGEPFPKAIPSPAPAHLRY----------FNAYGVTEMSCWQSLIEVQPGSSSCPP---PIWTSTGNLLSDTHV--SLTSQGEIVVSSATRICRVHPGQGTQHTEK--------GYSVF-TGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVLKE-----DRRVLGF-------HSQDIDPSRLSHLPPILRGVELVKVPRFPITRHGKIDLKLLLE---WKEETH---RKKVGLNVAKLWRQLTGIDPV-VDSNFVRDGGDSILAVE 375
            +AY+I TSG+TG+ K V +   S+   V++ +  +   +  D +L  S   FD    EI   +L    +H    +  L++    + I+   I  I LT SLF  +  ++   F   S LK++++GGE         +P+H+            +N YG TE + + + + +Q       P   P+  ST  +L+   V   + + GE+ V     + R + G+     EK        G  ++ TGDL  A+   DG   I +  R  + VK++G R+   E+   L Q  G     VL        + + G+       +++ +       LP  +    ++++   P+T +GK++ K L E     ++T+   R ++   +A +W+++ GI  + ++ +F   GGDSI A++
Sbjct: 1647 LAYIIYTSGSTGKPKGVMIEQRSVIRLVKNSN--YITFTPEDRLLMTSSIGFDVGSFEIFGPLLNGAALHLSDQQTFLDSHQLKRYIEHQGITTIWLTSSLFNHLTEQNEQTF---SQLKHLIIGGEAL-------SPSHVNRIRNVCPEVSIWNGYGPTENTTFSTCLHIQKTYELSIPIGRPVGNSTAFILNQWGVLQPVGAVGELCV-GGDGVARGYLGRPDLTKEKFVPHPFAPGDRLYRTGDL--ARWLSDG--TIEYVGRIDDQVKVRGYRVELGEIETALRQIDGVKEAAVLARTAQTGSKELFGYISVKAGTNAEQVRSLLARSLPNYMIPAYIIEMETLPLTSNGKLNRKALPEPDVASKQTYIPPRNELEEQLALIWQEVLGIQRIGIEDSFFELGGDSIKALQ 2043          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. SwissProt
Match: gi|160175923|sp|A5D7V7.1|S46A3_BOVIN (RecName: Full=Solute carrier family 46 member 3; Flags: Precursor)

HSP 1 Score: 74.3294 bits (181), Expect = 2.590e-12
Identity = 100/392 (25.51%), Postives = 164/392 (41.84%), Query Frame = 0

Query:  861 QIAVQKYVSELQAYNGIIQAIPSIIYALFAGPWSDRYGRRTLIAMACFGYIFNNGVFIINAYWFYELKAEYLLFECLQDCTGGSVCFFLGC-YAYISD-VSTRKNRTKRLAFLDGLF-PIGFFTGMSISGLIKKQWGFIPNFFLGMLAALLAVLYTVFVLKDSQDNRPEEVKAMLKEREAASTTTEPARKRNVCAQLFDLENLKASFRTTFKKRDNGMG---SALTLLIFTFVVEIFMTHGRGATMFLY-FRKEFAWTETQFGQFMALFGVVGLFTQYVAVPFLTDTCKIHDTLLGLGGVVGSGINLLIMALVRHDEWLYLGCVVAFLGPTITTSC-RSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPGMFLIISA 1244
            Q  VQK VS       I   IP ++        SD  GR+  + ++  G + N+    + +Y  + L  + L+         G+   FLG  +AYI D    +K RT R+A +D LF  +   TG+S SG   +  GF+ +F +  +A  + ++Y +  L+DS           +KE  + + +                E  K  F  T+    N  G   S   LL+FT +   F+T G      LY       W E   G   AL G V  F+ ++ + +L   C + D  +   G   + + + + A  R    ++L   + FL   +  S  RS+++K V   E G +F+ +  ++    + A   F  IY ATV    G   ++SA
Sbjct:   62 QEEVQKKVSLFNLEMDISGLIPGLVSTFVFLSHSDHGGRKFPLILSSVGALANSAWLCLLSY--FALPIQLLIASTFIGALFGNYTTFLGASFAYIVDQCKEKKQRTIRIAIIDFLFGVVSGLTGLS-SGYFIRGLGFVWSFLIVTVALFVNLIYILLFLEDS-----------MKESSSQNISVSWT------------ETFKNLFHRTYMLFKNASGEQQSLCCLLLFTMITYFFVTIGVSPIFVLYELDSPLCWDEVLIGYGSAL-GSVTFFSSFLGI-WLFSYC-MEDIHMAFIGTFTTMVGMAMTAFARTTLMMFL-VRLPFLFTVMPLSVLRSMISKVVHSTEQGTMFACLAFLETLGGITAVSTFNGIYSATVAWCKGFVFLLSA 423          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. nr
Match: gi|1325309487|ref|XP_023336366.1| (proton-coupled folate transporter-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 533.102 bits (1372), Expect = 7.158e-174
Identity = 255/445 (57.30%), Postives = 331/445 (74.38%), Query Frame = 0

Query:  805 KNLCSQITVEPILFLFSLSHGLYMIIAQTLYIEKVCNVNLNFTREICDNIYVHPDEQIAVQKYVSELQAYNGIIQAIPSIIYALFAGPWSDRYGRRTLIAMACFGYIFNNGVFIINAYWFYELKAEYLLFECLQDCTGGSVCFFLGCYAYISDVSTRKNRTKRLAFLDGLFPIGFFTGMSISGLIKKQWGFIPNFFLGMLAALLAVLYTVFVLKDSQDNRPEEVKAMLK-EREAASTTTEPARKRNVCAQLFDLENLKASFRTTFKKRDNGMGSALTLLIFTFVVEIFMTHGRGATMFLYFRKEFAWTETQFGQFMALFGVVGLFTQYVAVPFLTDTCKIHDTLLGLGGVVGSGINLLIMALV---RHDEWL-YLGCVVAFLGPTITTSCRSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPGMFLIISA 1244
            + +   ITVEPILF FS+S GLY+I+AQ+LYI+KVC VNLN T +IC NI  H  EQ+ VQKYVS LQ YNG++QAIP+I+YALFAGPWSD  GR+ LI  +CFGYIFNNGVFIIN  WF ELKAE+LLFECLQD TGG VCFFL CYAYISD+ST++ RTKRLAFLDGLFP GFFTGM++SG IK + GFI NF L ++ AL A+ + +F+LKDS+  RP+EV+A L   ++  +   +   K+  C  LFD+EN+K  F T FKKR+N + + + LL+  FV+E F+ +G+G T +LYFR++F+W   +FG ++ LFG VG+F Q+VAVPFLT+  K+HDT +G+  +    +  +++  +      EWL YL  V++FLG +ITT+ RSLVTKCVGP EVG VFSIMGA QA+VPL   P + +IYK T++TFPG FL+ SA
Sbjct:    4 RKIWKNITVEPILFFFSVSQGLYVIVAQSLYIQKVCQVNLNITEDICLNITNHKVEQLEVQKYVSVLQGYNGVLQAIPAIVYALFAGPWSDENGRKLLIVFSCFGYIFNNGVFIINTIWFKELKAEFLLFECLQDMTGGYVCFFLACYAYISDISTKEKRTKRLAFLDGLFPAGFFTGMALSGPIKDRIGFIGNFSLSIVFALSAMFWAIFILKDSRKQRPKEVQAYLDIVKKMENPDGQITAKKGKCLTLFDVENVKKGFTTVFKKRENNLRTYIVLLVIVFVLECFLINGKGPTQYLYFRRKFSWDMNKFGMYIGLFGFVGMFAQFVAVPFLTEKVKLHDTTIGVIAIASCIVQAIMVCFIPSGEEYEWLIYLAGVISFLGVSITTTTRSLVTKCVGPLEVGKVFSIMGAFQASVPLVGSPTYAYIYKGTIETFPGAFLLFSA 448          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. nr
Match: gi|1325309485|ref|XP_023336365.1| (proton-coupled folate transporter-like isoform X1 [Eurytemora affinis])

HSP 1 Score: 533.872 bits (1374), Expect = 7.302e-174
Identity = 255/445 (57.30%), Postives = 331/445 (74.38%), Query Frame = 0

Query:  805 KNLCSQITVEPILFLFSLSHGLYMIIAQTLYIEKVCNVNLNFTREICDNIYVHPDEQIAVQKYVSELQAYNGIIQAIPSIIYALFAGPWSDRYGRRTLIAMACFGYIFNNGVFIINAYWFYELKAEYLLFECLQDCTGGSVCFFLGCYAYISDVSTRKNRTKRLAFLDGLFPIGFFTGMSISGLIKKQWGFIPNFFLGMLAALLAVLYTVFVLKDSQDNRPEEVKAMLK-EREAASTTTEPARKRNVCAQLFDLENLKASFRTTFKKRDNGMGSALTLLIFTFVVEIFMTHGRGATMFLYFRKEFAWTETQFGQFMALFGVVGLFTQYVAVPFLTDTCKIHDTLLGLGGVVGSGINLLIMALV---RHDEWL-YLGCVVAFLGPTITTSCRSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPGMFLIISA 1244
            + +   ITVEPILF FS+S GLY+I+AQ+LYI+KVC VNLN T +IC NI  H  EQ+ VQKYVS LQ YNG++QAIP+I+YALFAGPWSD  GR+ LI  +CFGYIFNNGVFIIN  WF ELKAE+LLFECLQD TGG VCFFL CYAYISD+ST++ RTKRLAFLDGLFP GFFTGM++SG IK + GFI NF L ++ AL A+ + +F+LKDS+  RP+EV+A L   ++  +   +   K+  C  LFD+EN+K  F T FKKR+N + + + LL+  FV+E F+ +G+G T +LYFR++F+W   +FG ++ LFG VG+F Q+VAVPFLT+  K+HDT +G+  +    +  +++  +      EWL YL  V++FLG +ITT+ RSLVTKCVGP EVG VFSIMGA QA+VPL   P + +IYK T++TFPG FL+ SA
Sbjct:    4 RKIWKNITVEPILFFFSVSQGLYVIVAQSLYIQKVCQVNLNITEDICLNITNHKVEQLEVQKYVSVLQGYNGVLQAIPAIVYALFAGPWSDENGRKLLIVFSCFGYIFNNGVFIINTIWFKELKAEFLLFECLQDMTGGYVCFFLACYAYISDISTKEKRTKRLAFLDGLFPAGFFTGMALSGPIKDRIGFIGNFSLSIVFALSAMFWAIFILKDSRKQRPKEVQAYLDIVKKMENPDGQITAKKGKCLTLFDVENVKKGFTTVFKKRENNLRTYIVLLVIVFVLECFLINGKGPTQYLYFRRKFSWDMNKFGMYIGLFGFVGMFAQFVAVPFLTEKVKLHDTTIGVIAIASCIVQAIMVCFIPSGEEYEWLIYLAGVISFLGVSITTTTRSLVTKCVGPLEVGKVFSIMGAFQASVPLVGSPTYAYIYKGTIETFPGAFLLFSA 448          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. nr
Match: gi|1325309839|ref|XP_023336534.1| (uncharacterized protein LOC111707632 [Eurytemora affinis])

HSP 1 Score: 389.037 bits (998), Expect = 3.504e-119
Identity = 212/472 (44.92%), Postives = 301/472 (63.77%), Query Frame = 0

Query:  804 LKNLCSQITVEPILFLFSLSHGLYMIIAQTLYIEKVCNVNLNFTREICDNIYVHPDEQIAVQKYVSELQAYNGIIQAIPSIIYALFAGPWSDRYGRRTLIAMACFGYIFNNGVFIINAYWFYELKAEYLLFECLQDCTGGSVCFFLGCYAYISDVSTRKNRTKRLAFLDGLFPIGFFTGMSISGLIKKQWGFIPNFFLGMLAALLAVLYTVFVLKD----------SQDNRPEEV-------KAMLKE-REAAS-----TTTEPARKRNVCAQLFDLENLKASFRTTFKKRDNGMGSALTLLIFTFVVEIFMTHGRGATMFLYFRKEFAWTETQFGQ---FMALFGVVGLFTQYVAVPFLTDTCKIHDTLLGLGGVVGSGINLLIMALVRHD-EWL-YLGCVVAFLGPTITTSCRSLVTKCVGPFEVGAVFSIMGAIQATVPLAAFPFFGFIYKATVDTFPGMFLIISACFY 1247
            +K +   I+VEP    FS+  GLY ++   LY++KVC VNLN ++EIC+N+  + + +I VQK VS L+ Y+ ++QA P I++ALFAGPWSD +GR+ LI  +CFGYI NN VFIIN  WF E  AEYLL E LQDCTGG VCFFLGCY+YISDVS+ + RTKRLAF DGL PIG + G+++SG IK  +G+I NF   ++ AL A+ + +F LKD          S++ +  EV       K M +E RE+A         +   K  VCA LFDL+++K  F   FKKR       L LL+  F+++ F  +GRG++ +LY+R++F W   +F     F  L+G VG+F   V +P L+ T  +HDT +G+   +   + L+++     D EW+ YL   ++ L P ITT CRSL+TKC+ P EVG VFSI+GA+Q +V + + P +G++YK +VD FPG++L+ +A  Y
Sbjct:    3 IKEIWRNISVEPCYLFFSICQGLYTMVVANLYMDKVCQVNLNISQEICNNLSQYKEIEIEVQKEVSVLRGYSSLLQAAPGILFALFAGPWSDTHGRKYLIVCSCFGYIINNTVFIINVIWFKEFTAEYLLLEVLQDCTGGFVCFFLGCYSYISDVSSSQTRTKRLAFFDGLSPIGLYIGLAVSGPIKVNFGYIANFVSAIIFALAAMFWALFFLKDFHVPSEEIIESEEMKEMEVLNGNRVSKEMEEEKRESADLNLNIKAVKGRSKSKVCA-LFDLKHVKDGFSAMFKKRAGKFRKYLLLLVLCFLMQSFCYYGRGSSDYLYYRRQFNWNTNEFSMYKCFQGLWGSVGMF---VVIPVLSRTFHLHDTTIGVIATIAMIVQLIVVCFTPPDYEWMVYLTAAISLLSPGITTLCRSLITKCIEPTEVGKVFSILGALQGSVHVFSGPGYGYLYKMSVDIFPGLYLLCTASVY 470          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. nr
Match: gi|1022762639|gb|KZS08336.1| (Acyl-CoA synthetase family member 4 [Daphnia magna])

HSP 1 Score: 342.428 bits (877), Expect = 5.841e-97
Identity = 248/844 (29.38%), Postives = 403/844 (47.75%), Query Frame = 0

Query:    3 ERADVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFE----LVKSGPAFGAQSTLKYVVLGGEPFPKAIPSP---APAHLR-YFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSDTHVSLTSQGEIVVSSAT-----------RICRVHPGQGTQHTEKGYSVFTGDLGEAQCTGDGQQ----AILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVLKEDRRVLG-------FHSQDIDPSRLSHLPPILRGVELVKVPRFPITRHGKIDLKLLLEWKEETHRKKVGLN----VAKLWRQLTGIDPVVDSNFVRDGGDSILAVEFINEIEDATNNPSVQSLLELLLNGQFGEFQDQIMARIGDSSILAQSRVKEHHLNSCSDMDISRTPLDRSADHTQISILGRGFR--RGP--IRSTIDIGSTDLKVLWSVDLNKCIDATPLIVKDKEDTVIFIGSHSGLFAAIHMDSGRTRWTRNLGDRIESSANCSPDGRLIFIGCYDCAMYCLDARSGDILWSFKTGGIIKSIPAVDGERVIFGSYDHFVYCLNSITGTLLWAKNIARSSILTKALTLVDGSVIACTLSGHVSKRDTQEGANMWTTQLDGPIFSSPLCIGTWG--YFATAKGLLSKLCLASSELVWTFDAQGPIFSSLVPSGSDILLGTHACEVLCVDSTSGLCRWRVKHTDRVFATPLLTSSQMCVSLSTDGQIIVSKDGAIVKMVYFPVKDRLQGGFFSSPLAHNGQIFQNLKDTLNMCCAGLKN 806
            E  ++AY+I TSG+TG  K+V V  + I  N+ D    F +    D +   SP TFDP  +++ LA+ A   +  V   IK   +  +++I+   + FIQ TPS F     L K+   + + + L+ VVLGGEPFP  I      AP +   ++N YG+TE+SCW +L +++  S+  P  +    G  LS+T + + ++ E  +++             R+C +        T+  +          + TGD  Q     + +  R    VK  G+R++   + + L+ + G    C    D   L        F  + +      +   I +  E+  +  FP+++HGKID K LL+  +     +  LN    +AK W   TG     +SNF+  GG+S+ A+                          F + Q+Q+   +     +  ++  +H ++   +     T +D   +   I I  RG +  + P  I  T +  S +LK +W   L KCIDA+PL+++ KE   +F+GSHS  F+A+ +D+G+  WT  L +RIESSA C  DG  IF+GCYD  +YCLDAR+G+I W FKTG  IKS P   G  VIFGS+D  +YCL+   G L+W + I+  S+        +  +++ +L G  S      G  +W+ +L  P+FS+P+         FA+  G ++     + ++VW F A GPIFS+       IL G+H   + C+   SGL  WRV  T  ++++P   S  +C   +   +++    G ++       + RL G  FSSP+  +  +    +D  N+ C  L N
Sbjct:  149 EEWNIAYMIHTSGSTGAPKTVRVPHSCILPNILDLRENFNIQQ-NDVIFMASPFTFDPSIVDLFLALSAGAKLIQVESSIKAMPLRLAEIIRDEGVTFIQATPSFFRQFDHLSKTHLLYPS-TPLRTVVLGGEPFPNPIALEEFRAPGNCTVFYNIYGITEVSCWATLSKIE--SNDLPADL----GYPLSETDLRIVNENEEDITTENGGEGFLMLGGRRVCLIDEETPDSITKPMF----------RATGDRVQFLNGKLYYRGRTDETVKRMGKRLNLHSI-ELLALSSGFVQQCCAFLDSNQLLILVCTGIFTVEQLSQYFQENAATIEQPDEIRVLDTFPVSKHGKIDRKKLLQLVQNLRVPEKTLNWETCLAKEWMMATGKTTTDESNFILSGGNSLSAIRL------------------------FSKVQEQVARPLPLLIDILLNQSWKHVVDHIREETRKNTEVDSLLNQPTIKIFQRGKQAAQSPKHIDQTSEEASFELKQIWKFSLKKCIDASPLVIQRKEVDAVFVGSHSHHFSAVRLDNGQEIWTTRLPERIESSATCDMDGSNIFVGCYDGHLYCLDARTGNICWHFKTGDAIKSSPVCTGFSVIFGSHDRILYCLSVARGDLIWKRTISAGSLFASPAWDGNNRILSASLDGTCSALTVATGQLLWSMKLATPVFSTPVWCSKLERCLFASVDGFMNCCAADTGDIVWRFRAAGPIFSTPTIREQRILFGSHDNFLYCLHEESGLQLWRVGFTSPIYSSPFAASVIICSDTNGYLKVLEFSTGRLL------AQSRLDGQVFSSPVMVDHLVIVGCRDD-NLYCFQLSN 942          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. nr
Match: gi|321459442|gb|EFX70495.1| (hypothetical protein DAPPUDRAFT_328037 [Daphnia pulex])

HSP 1 Score: 317.775 bits (813), Expect = 1.300e-88
Identity = 239/783 (30.52%), Postives = 372/783 (47.51%), Query Frame = 0

Query:    3 ERADVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFELVKSGPAFGA------QSTLKYVVLGGEPFP--KAIPS-PAPAHLR-YFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSDTHVSLTSQGEIVVS-----------SATRICRVHPGQGTQHTEKGYSVFTGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPE---LAQDLSQALGCPFHCVLKEDRRVLGFHSQDIDPSRLSHLPPILR----GVELVKVPR----FPITRHGKIDLKLLLEWKEETHRKKVGLN----VAKLWRQLTGIDPVVDSNFVRDGGDSILAVEFINEIEDATNNPSVQSLLELLLNGQFGEFQDQIMARIGDSSILAQSRVKEHHLNSCSDMDISRTPLDRSADHTQISILGRGFRRGPIRSTIDIGST-DLKVLWSVDLNKCIDATPLIVKDKEDTVIFIGSHSGLFAAIHMDSGRTRWTRNLGDRIESSANCSPDGRLIFIGCYDCAMYCLDARSGDILWSFKTGGIIKSIPAVDGERVIFGSYDHFVYCLNSITGTLLWAKNIARSSILTKALTLVDGSVIACTLSGHVSKRDTQEGANMWTTQLDGPIFSSPLCIGTWG--YFATAKGLLSKLCLASSELVWTFDAQGPIFSSLVPSGSDILLGTHACEVLCVDSTSGLCRWRVKHTDRVFATPLLTSSQMCV 746
            E  ++AY+I TSG+TG  K V V    +  N+ D  ++F +    D +   SP TFDP  +++ L       +  V   IK      +++I++  + F+Q TPS   L++ GP           S L+ +VLGGE FP  K +    +P H   ++N YG+TE+SCW +L +++         I    G+ L +T + + ++ E  +               RIC +   +     EK     TGD  E     +G+  + +  R    VK  G+R++      LA        C   C L +  R+L      I  S + HL    +     VE   V R     P+++HGKID K LL+  + T +    LN    +A  WR  TG     +SNF+  GG+S+ A+  +N+I++ T+  S+  L ++LLN  + E  D I               ++  LN      IS   +  +   +++ I  R      +   I   ST +LK +W  +L KCIDA+PL+++ KE   +FIGSHS  F+A+ +DSG+  WT  L +RIESSA+C  +G  IF+GCYD  +YCLDA +G I W + TG  IKS P   G  V+FGS+D  +Y L+   G LLW K I+  S+           +++  L G  S      G  +WT +L  PIFSSP+          A+  G ++     + ++VW + A GPIFS+   +   I+ G+H   + C+D  S   R        VF++P++    + V
Sbjct:  149 EEWNIAYLIHTSGSTGTPKVVRVPHTCLLPNILDLRKIFNIQQ-TDVIFIASPFTFDPSIVDLFLTFSTGARMVLVKPSIKAMPSKLAEIIRKEGVTFLQATPSF--LLQFGPELSKTHLLYPSSPLRIIVLGGEAFPALKTLSEFSSPGHSTVFYNIYGITEVSCWATLSKIESNE------IQVDLGHALGETDLRVINEDEEEIHRDQGGEGYLWLGGRRICLLD-DETPDSIEKPVFRPTGDRVEFL---NGK--LYYRGRKDETVKRMGKRLNLHSVEILALSSGLVQQC---CALLDSNRLLVLVCNGI--SVIDHLLQFFQENAAAVEQPDVIRILDSLPMSKHGKIDRKKLLQIIQTTPKHDGKLNWETILANEWRNATGQTATDESNFILSGGNSLSALRLVNQIQEETSR-SLPLLTDILLNQNWKETVDYI---------------RKESLNG----PISSLQIQVAHPKSKVEIFQRSQPSSKVTDVISKASTLELKQIWKFNLKKCIDASPLVIRRKEGDAVFIGSHSHRFSAVKLDSGQEIWTTELPERIESSASCDMNGVNIFVGCYDGNLYCLDALTGRICWHYSTGDAIKSSPVCTGPSVVFGSHDRTLYSLSVARGELLWRKTISEGSLFASVAWDGSNRILSAALDGTCSALSVTSGELLWTIKLSTPIFSSPVWFSKSERCLLASVDGFINCCSADTGDIVWRYQAAGPIFSTPTVADKRIIFGSHDHFLYCLDEESAQTRL----DGEVFSSPVMVDQLVVV 887          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. nr
Match: gi|1325268435|ref|XP_023345997.1| (acyl-CoA synthetase family member 4-like [Eurytemora affinis])

HSP 1 Score: 313.923 bits (803), Expect = 7.280e-87
Identity = 241/822 (29.32%), Postives = 398/822 (48.42%), Query Frame = 0

Query:    3 ERADVAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFELV----------KSGPAFGAQST-----LKYVVLGGEPFPKAIPSPAP----AHLRYFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSDTHVSLTSQGEIVVSSATRICRVHPGQGTQHTEKGYSVFTGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVLKEDRRVLGFHSQDIDPSRLSHLPPILR----GVELVKVPR-------FPITRHGKIDLKLLLEWKEETHRKKVGLNVA---KLWRQLTGIDPVVDSNFVRDGGDSILAVEFINEIEDATNNPSVQ-SLLELLLNGQFGEFQDQI-MARIGDSSILAQSRVKEHHLNSCSDMDISRTPLDRSADHTQISILGRGFRRG-----PIRSTIDIGSTDLKVLWSVDLNKCIDATPLIVKDKEDTVIFIGSHSGLFAAIHMDSGRTRWTRNLGDRIESSANCSPDGRLIFIGCYDCAMYCLDARSGDILWSFKTGGIIKSIPAVDGERVIFGSYDHFVYCLNSITGTLLWAKNIARSSILTKALTLVDGSVIACTLSGHVSKRDTQEGANMWTTQLDGPIFSSPLCIGTWGYFATAKGLLSKLCLASSELVWTFDAQGPIFSSLVPSGSDILLGTHACEVLCVDSTSGLCRWRVKHTDRVFATPLLTSSQMCVSLSTDGQIIV-SKDGAIVKMVYFPVKDRLQGGFFSSPL 783
            +  DVAY+IQTSGTTG  K + V+ +SI  N++DF+  F L    D V   SP TFDP  ++I L++++   +  V+   K+   ++S ++  +R++ IQ TPSL + +           S P+   + T     L+ + +GGE     +              +  YG+TEMS WQS+++++P      P ++    NL++ T +    +  I++ S TRICR   G    + E      TGD+GE +  GD    +++  R  N +K+ GR+IS   + + LS+ L     C + E+   +      I   + S +  IL      +E  ++P+       FP+  HGKI+ K+L +      R   G  V+    LW +L  I P    NF+  GGDS LAV   N + +    P +  + L+ +L+ QFG   + I  A+   +SI+ + R+ +     C    + +  LD   D  +  I G    +G        +         +  W VD  KCID++PL+++  E   + +GSHSG    I ++ G   W   L DR+E S   S +   I+IGCYD  +Y L   +G++ W FKT GI+K  P +    + FGSYD   Y LN+  G L+W    +  SI +  + + +G V+A TL G ++   +  G  +W+  L  P+FS+PL  G   Y A+    +  +   S  +VW  +   PIFSS +   S++LLG++   V  + +  G   WR   ++ VF++     +   +  +TDG I+V +  G I+      +++++ G  FSSP+
Sbjct:  139 DTGDVAYIIQTSGTTGEPKPILVTNSSILPNIQDFNAAFQLKK-TDVVFLSSPLTFDPSIIDIFLSLVSGAQLLVVTTTEKIKQDLYSTLLIENRVSVIQCTPSLLQNICRPQTSLINTNSSPSMIVKPTVEKTSLRILAVGGEKCSSQVREILQRLCLEGTAVYQLYGLTEMSVWQSMVKLEPKEIETMP-VYIKDRNLITQTEIQ-CEENAIIIYSKTRICRYKDGISLSYIE------TGDMGEWR--GD---ELIWVGRSDNIIKIHGRKISLERMEETLSKVLETQILCAVSENGESISAFL--IYTEKWSQVERILERCREQLESWQIPQNIVFLNEFPVNNHGKINRKILFK-TPAVSRNISGDLVSWFSDLWLKLLKISPGSMDNFILCGGDSFLAVLVYNIMVERI--PGLHPTFLQDILSAQFGALCETIAQAQSRKNSIVIKDRISDDSNQQCEPRALKKVKLD-VQDIWEFKIKGVFRLKGRESCSRSFADSSSSPIGFRQDWKVDFEKCIDSSPLLLRVGEKNRVVVGSHSGWVKCIEVEDGEVVWATRLSDRVEGSP--STEFSSIYIGCYDNHVYSLSLSTGEVQWRFKTQGIVKCTPLIHNGFIYFGSYDKLYYKLNT-NGELIWKIKPSDGSIYSSPINMSNGFVLA-TLDGEIA-LISFSGDTLWSLNLGHPVFSTPLWDGNQLYVASVDKFIYAVS-ESGSIVWKVETGSPIFSSPILYKSNLLLGSNDGYVYNI-TPGGEINWRTDLSNPVFSSIDFDPNGRILCATTDGSILVLNSSGRIL------IREKVPGNVFSSPI 927          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. nr
Match: gi|1009547470|ref|XP_015909614.1| (acyl-CoA synthetase family member 4 [Parasteatoda tepidariorum])

HSP 1 Score: 286.96 bits (733), Expect = 4.394e-77
Identity = 252/862 (29.23%), Postives = 391/862 (45.36%), Query Frame = 0

Query:    7 VAYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLF-----ELVKSGPAFGAQSTLKYVVLGGEPFP-----KAIPSPAPAHLRYFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSDTHVSL--TSQGEIVVSSATRICRVHPGQGTQHTEKGYSVF--TGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQDLSQALGCPFHCVLKEDR-------------RVLGFHSQDIDPSRLSHLPPILRGVELVKVPRFPITRHGKIDLKLLL--------EWKEETHRKKVGLNVAKLWRQLTGIDPVV-DSNFVRDGGDSILAVEFINEIEDATNNPSVQSLLELLLNGQFGEFQDQIMARIGDSSILAQSRVKEHHLNSCSDMDISRT--PLDR------SADHTQISILGR---------GFRRGPIR---STIDIGSTDLKVLWSVDLNKCIDATPLIVK--DKEDTVIFIGSHSGLFAAIHMDSGRTRWTRNLGDRIESSANCSPDGRLIFIGCYDCAMYCLDARSGDILWSFKTGGIIKSIPAVDGERVIF-GSYDHFVYCLNSITGTLLWAKNIARSSIL-TKALTLVDGSVIACTLSGHVSKRDTQEGANMWTTQLDGPIFSSPLCIGTWGYFATAKGLLSKLCLA-SSELVWTFDAQGPIFSSLVPSG--SDILLGTHACEVLCVDSTSGLCRWRVKHTDRVFATPLL---TSSQMCVSLSTDGQIIV--SKDGAIVKMVYFPVKDRLQGGFFSSPLAHNGQIFQNLKDTLNMC 800
            +AY++QTSG+TG+ K V V    I  N+ D    F + +  D++L  SP TFDP  ++I L   +   +  +   IK      ++VI  ++I  +Q TPSL      E ++       +S+LK +  GGE  P     +   SP    +  FN YG+TE+SCW +  ++    +     I    G LLSDT++ L    +GE+ + S  R C V         EK  +VF  TGD+      G+      F  R  N +K +GR++S   L   L    G   + V  + +             R    + +     +L    P    +EL  VP  P+T HGK D + LL        E  +   ++     +++LWR+ T       DSNF+  GGDSI A++  + IE A    +V +L++ +LN  F      + + + D+  +    VKE   N+C  +  + T  P  R      ++D+    +  +         G     I      I   S  L  ++  DL KCIDA+P IV     ++   +IGSH+G+F+ I++      W   L +RIESSA  S  G  +F+GCYDC +YCLD++ G ILW F+TG  +K  P V  E +IF GS+D  VY ++ I G LLW   ++  SI  + AL     +V   TL G +S      G  +W+ +   P+FSSP  + +  Y  +  G +  LC+  + +L   +   GP+FSS   +     I+ G H   + C++ TS L  W       ++ATP +            ST G I +   K+G ++     P      G  FSSP+  N  +    +D    C
Sbjct:  166 IAYIVQTSGSTGQPKIVQVPHQCILPNILDLKSQFSI-NTDDNILLCSPLTFDPSIIDIFLFCASGCTLTIIPDEIKSIPKKLAEVIIDNKITILQGTPSLISSLGDEFIQKS-LLSRESSLKTLAFGGEICPSINKLRKWISPENKTM-LFNLYGLTEVSCWATCHKINVADTFVNAAI--PLGKLLSDTNLKLDNNDEGELQIGSHKRWCIV---DDESFPEKDTAVFRRTGDIVIQDSFGN----FYFKERKDNTIKYKGRKLSLEILHDTLDVEEGLSNYSVYFDQKHFKLLLYLVLKKSRTFSENERKTLIMKLRKNCPNAPPIELFTVPYIPLTSHGKTDYRRLLLLAKRKVREINKTQLKESCSEVISELWREFTNQPQFTEDSNFIISGGDSISALQLSSNIEWAFQ-INVPNLVDRILNSSFCNVVKLVQSHLHDAEKIT---VKE--ANNCDVLQKNPTLFPSKRKCIKRLNSDNQCYQVFSKQECVTRCACGCDHSNIMYNLKNIPHSSFSLIPMFKFDLKKCIDASPCIVHYLKSDELFCYIGSHAGIFSCINITKEDMEWETVLPNRIESSACLSSCGNFVFVGCYDCHLYCLDSKYGTILWKFQTGAEVKCSPIVSSENIIFVGSHDKHVYAID-INGELLWKSKVSSGSIFASPALHEEIKAVCIATLDGTISFLKMDSGDLIWSFEYQKPLFSSPRILSSLIYVGSTDGHM--LCMDLTGQLKMKYKTNGPVFSSAANASFPGIIIFGCHDGIIYCLNETSDL-LWNFSCDAPIYATPFIFKWNGENFVAVASTKGSIYILKLKNGKLIMSYNCP------GEIFSSPVIVNNLLLIGCRDNNVYC 999          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. nr
Match: gi|443693394|gb|ELT94770.1| (hypothetical protein CAPTEDRAFT_220657 [Capitella teleta])

HSP 1 Score: 281.182 bits (718), Expect = 2.019e-75
Identity = 233/822 (28.35%), Postives = 366/822 (44.53%), Query Frame = 0

Query:    8 AYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVIQRHRIAFIQLTPSLFELVKSGPAFGAQSTLKYVVLGGEPFPKA------IPSPAPAHLRYFNAYGVTEMSCWQSLIEVQPGSSSCPPPIWTSTGNLLSDTHVSLTSQGE---IVVSSATRICRVHPGQGTQHTEKGYSVFTGDL--GEAQCTGDG---QQAILFHT-RCQNFVKLQGRRISFPELAQDLSQALGCPFHCVLKEDRRVLGFHSQD--------IDPSRLSHLPPILRGVELVKVPRFPITRHGKIDLKLLLEWKEETHRKKVGL-NVAK----LWRQLTGIDPVVDSNFVRDGGDSILAVEFINEIEDATNNPSVQSLLELLLNGQFGEFQDQIMARIGDSSILAQSRVKEHHLNSCSDMDISRT-PLDRSADHTQISILGRGFRRGPIRSTIDIGSTDLKVLWSVDLNKCIDATPLIVKDK-EDTVIFIGSHSGLFAAIHMDSGRTRWTRNLGDRIESSANCSPDGRLIFIGCYDCAMYCLDARSGDILWSFKTGGIIKSIPAV--DGERVIFGSYDHFVYCLNSITGTLLWAKNIARSSILTKALTLVDGSVIACTLSGHVSKRDTQEGANMWTTQLDGPIFSSPLCIGTWGYFATAKGLLSKLCLASSELVWTFDAQGPIFSSLVPSGSDI-----LLGTHACEVLCVDSTSGLCRWRVKHTDRVFATPLLTSSQMCVSLSTDGQIIVSKDGAIVKMVYFPVKDRLQGGFFSSP--LAHNGQIF 790
            AY++ TSGTTG  K V V    I  N+     +F L S +D +   SP TFDP  +EI  +  +   +   S++I+      S ++ +HRI+ +Q TPSL    +          L+ + LGGE FP          S        +N YG+TE+S W S  ++   S         S G  L  T   L   GE   + V    R+C V+  +              DL  G  + TGD    +Q  +F+  R  N +K  G+R++  E+ + ++ +LG    CV   D  VL    Q         +  +    LP      ++     FP+T HGKID + LL  + ++H   +   N++K    +W ++  + P    NF+  GG+S+ A+ F N I     N +   +L+ +L+   G+    +       +      V+   +N    M   R   L     H  +++   G                +KVLWS D +KC+DA+PLI+ +K +D+++ IGSHSG   ++ + SGR  WT  L DRIESSA     G+   +GCYD  +Y +   +G ILW + TG  +K  P V  D   +  GS+DHF+Y LN       W  N+  S   +  +     ++IA +LSG + K     G  +WT   + PIF+SP C G + Y A   G L++L  +  +  W++    PIFSS +   + I     + G H   + CV S  G   W    T  ++A P +    + ++ S      V  +    +      + RL G  FSSP  ++ NG ++
Sbjct:  158 AYIVTTSGTTGLPKIVKVPHKCILPNIIHLSEIFCLCS-SDVIFQSSPLTFDPSVVEIFCSFFSGASLLCTSNKIRKQPQTLSAILIQHRISILQTTPSLIMKFEHNALRAIMHNLRILALGGEKFPSTKTVASWFNSDDERRPEIYNLYGITEVSSWASCFKLT-DSDLRREDFEASIGEPLMGTTFELKQNGEHNELCVGGDDRVCLVNDEE--------------DLIPGFFRATGDSVIRKQGFIFYVGRLDNQIKRNGKRMNLDEI-ERVALSLGDVTQCVALWDSPVLRLCIQSDCERTIGCVQNAIKQRLPAHYYPDQVTLCAHFPLTSHGKIDQRKLL-LQVQSHETGISCGNISKSFLDIWEEILAVKPSATDNFIESGGNSLQALNFYNLICRTIRNEN-PLILDYILHSSVGKVIKYLKGIPMGETSKDDVSVEAESMNYVMSMSRGRNHSLGLQGSHDAVNLKRSG---------------SMKVLWSHDTHKCVDASPLILMNKSKDSIVLIGSHSGELCSLDLHSGRPAWTITLPDRIESSACVFLSGKYAAVGCYDAGVYFVSITTGAILWCYATGAPVKCSPVVHPDNALIFIGSHDHFLYALNLQERKCSWKLNLGGSVFASVCVVDEAENLIAASLSGILFKLKAINGIVIWTYNCEKPIFASPCCHGDYVYVACVGGFLTQLN-SFGQKQWSYKTSAPIFSSPITHAASIKDHCVIFGCHDHRLYCV-SAGGAPLWTYSATSTLYAAPFVHPPSVWITSSEGNLYEVDLENGKQRG-----ECRLPGEVFSSPVCVSLNGMVY 938          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. nr
Match: gi|1188105406|ref|XP_020774582.1| (acyl-CoA synthetase family member 4 [Boleophthalmus pectinirostris])

HSP 1 Score: 276.559 bits (706), Expect = 2.394e-73
Identity = 253/885 (28.59%), Postives = 391/885 (44.18%), Query Frame = 0

Query:    8 AYVIQTSGTTGRRKSVCVSWASIEANVRDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVI-QRHRIAFIQLTPSLFELVKSGPA------FGAQSTLKYVVLGGE--PFPKAIP--SPAPAHLRYFNAYGVTEMSCWQSLIEVQP----GSSSCPPPIWTSTGNLLSDTHVSLTSQ---------GEIVVSSATRICRVHPGQGTQHTEKGYSVFTGDLGEAQCTGDGQQAILFHTRCQNFVKLQGRRISFPELAQ-----DLSQALGCPFHCVLKEDRRVLGFHSQDIDPSRLSHLPPIL---------------RGVELVK-----------------VPRFPITRHGKIDLKLLLEWKEETHRKKV---------GLNVAKLWRQLTGIDPVV----DSNFVRDGGDSILAVEFINEIEDATNNPSVQSLLELLLNGQFGEFQDQI-----MARIGDSSILAQSRVKEHHLNSCSDMDISRTPLDRSADHTQISILGRGFR---------RGPIRSTIDI--------GSTDLKVLWSVDLNKCIDATPLI-VKDKEDTVIFIGSHSGLFAAIHMDSGRTRWTRNLGDRIESSANCSPDGRLIFIGCYDCAMYCLDARSGDILWSFKTGGIIKSIPAVDGER--VIFGSYDHFVYCLNSITGTLLWAKNIARSSILTKALTLVD-GSVIACTLSGHVSKRDTQEGANMWTTQLDGPIFSSPLCIGTWGYFATAKGLLSKLCLASSELVWTFDAQGPIFSS--LVPSGSDILLGTHACEVLCVDSTSGLCRWRVKHTDRVFATP-LLTSSQMCVSLSTDGQIIVSKDGAI----VKMVYFPVKDRLQGGFFSSPLAH 785
            AYV+ TSGTTG  K V V    I  N+ D   +F +    D V   SP TFDP  +E+ +A+ +   +  V   +K      +Q++ + H+   +Q TPSLF   + GP+        A S+L+ ++LGGE  P PK +            +N YG+TE+SCW S  +V       S S P P+    G  LS T V +  +         G++ +    R+C +           G    TGD    +   DGQ  + F  R    +K  G+R+S  E+ Q      L +A     H    E+ R++ F    I  S+L   P +L                G  ++K                 VP  P+T HGK+D+K L+    +THR  +          L +  LW+   G+   +    DSNF+  GGDS+ A+ F  ++  AT   +  +L+E++L+G F +    I     ++ + ++  + Q   K H  N    +           D     ++ RG +          G +++  DI         +  LK+ W  D  +C+DA+P+I V+    + +FIGSHS    A+ +DSG   W R LGDRIE+SA  S  G L+ +GCYD  +Y L+  SG+  W FKTG  +KS PAVD E   V  GS+D  VY L+  T    W  +    ++ +          +   TL G +   +      +W+   D P FSSP+   T     +A G +    L   +L+W F  Q P+FSS  L P    +L G+H   + C++   G   WR + + RV+++P +  +S   V  +  G  + S DG++    V+         L G  FSSP+ H
Sbjct:  177 AYVLHTSGTTGNPKIVKVPHKCIRPNICDLSLMFQIKK-GDLVFLASPLTFDPSVVEMFMALTSGATLLIVPALLKKMPKRLTQLLFKNHKTTILQATPSLF--YRFGPSSLKEDVLSASSSLRILLLGGESCPTPKQLADWKHRDNKTEIYNIYGITEVSCWASCYKVLEKELQSSQSSPVPL----GVQLSGTEVEVRDEHGVVMKEGDGQVFIGGEHRVCLL---DDEDTLVSGTMRATGDWVRLK---DGQ--MHFLGRRDRMIKRNGQRLSLDEVQQLILTAPLVEACAVGIH----ENHRLIAF----IVTSKLESSPVVLCDATMREEQEGEVGKVGRTILKELSQTLAHFSVPDTVLIVPEIPLTAHGKVDMKALMR-TYQTHRSGLLGSSQRDFTKLTLESLWQTSLGLPKEIPINEDSNFLVSGGDSLKALIFHEDLL-ATTGLTSPNLVEIILDGTFADILHHISEVNLVSSLENNGQVKQEATKRHLENPYEAIAKRERKTSSEKDIPVFKVIKRGSQIIEMPNTKSTGHLQTIEDITLSKIGESKTVSLKMHWLSDTGRCVDASPVILVQQMARSTVFIGSHSHRIQALDLDSGSLVWERVLGDRIEASATVSSCGSLVIVGCYDGGVYFLNVSSGETEWLFKTGDTVKSCPAVDAETGLVFVGSHDGQVYALDPKTRHCAWKHSCGGGAVFSSPFIHPSLRHLFVATLGGRLLCLNMDTAEELWSYSKDVPFFSSPVSSDTHVVIGSADGNICSFSL-DGQLLWQFLTQAPVFSSPCLTPDLLQVLCGSHDGHIYCLNICDGALIWRFETSGRVYSSPCVFGTSAPRVRGTLVG--LASTDGSVWILDVETGLKRASHTLPGELFSSPVVH 1033          

BLAST of hypothetical protein DAPPUDRAFT_328037 vs. nr
Match: gi|1176291646|ref|XP_020648184.1| (acyl-CoA synthetase family member 4 isoform X1 [Pogona vitticeps] >gi|1176291648|ref|XP_020648185.1| acyl-CoA synthetase family member 4 isoform X1 [Pogona vitticeps] >gi|1176291650|ref|XP_020648186.1| acyl-CoA synthetase family member 4 isoform X1 [Pogona vitticeps])

HSP 1 Score: 274.248 bits (700), Expect = 2.100e-72
Identity = 258/921 (28.01%), Postives = 397/921 (43.11%), Query Frame = 0

Query:    3 ERADVAYVIQTSGTTGRRKSVCVSWASIEANV---RDFHRLFPLASVADSVLSLSPPTFDPFYLEILLAVLAHRPIHFVSHRIKLNAVMFSQVI-QRHRIAFIQLTPSLF-----ELVKSGPAFGAQSTLKYVVLGGEPFP--KAIPSPAPAHLR--YFNAYGVTEMSCWQSLIEVQPGSSSCP-----------PPIWTSTGNLLSDTHVSLTSQGEIVVSSATRICRVHPGQGTQHTEKGYSVFTGDLGEAQCTGD----GQQAILFHTRCQNFVKLQGRRISFPELAQ---DLSQALGCPFHCVLKEDRRVLGFHSQDIDPSR--LSHLPPILRGV----ELVKVPRFPITRHGKIDLKLLLEW----------KEETHRKKVGLNVAKLWRQLTGIDP-----VVDSNFVRDGGDSILAVEFINEIEDATNNPSVQSLLELLLNGQFGEFQDQIMARIGDSSILAQS-------RVKEHHLNSCSDMDI---SRTPLDRSADHTQISILGRGFR-------------------RGPIRSTIDIGSTD---------------------------LKVLWSVDLNKCIDATPLIV---KDKEDTVIFIGSHSGLFAAIHMDSGRTRWTRNLGDRIESSANCSPDGRLIFIGCYDCAMYCLDARSGDILWSFKTGGIIKSIPAVDGER--VIFGSYDHFVYCLNSITGTLLWAKNIARSSILTKA-LTLVDGSVIACTLSGHVSKRDTQEGANMWTTQLDGPIFSSPLCIGTWGYFATAKGLLSKLCLA-SSELVWTFDAQGPIFSSLVPS--GSDILLGTHACEVLCVDSTSGLCRWRVKHTDRVFATPLLTSSQ------MCVSLSTDGQIIVSKDGAIVKMVYFPVKDRLQGGFFSSPLAHNGQIFQNLKDTLNMC 800
            ++  +AY++ TSGTTG  K V V    I  N+   RD  ++ P     D+V   SP TFDP  +E+ +A+ +   +  V  +IK+     S+V+ QRH ++ +Q TP+L      + +KS     A S+L+ + LGGE FP    + S      R   FN YG+TE+SCW +  +V     S             P + T+     ++    L  +G++ +    R+C +                T  +G  + TGD        + F  R  N +K  G+R+   ++ Q   D S+   C      +E + +L    + I   R  L++L   L G     EL+ +   P T HGKID+  L +           K +  RK++   +  LW+ L  +       + DS F+  GGDS+ +++F  EIE      +V SLLE++L+  F E    ++  +  S  L  S       ++ E     CS   +   S   L    D      + RG R                      ++S  D+ +T                            L + W  DL KC+DA+PL+V    DK    ++IGSHS +  AI + SG+ +W R L DRIESSA  S  G  I +GCY   +Y L +  G++ W+FKT   +KS PAVD     V  GS+D  VY L+      +W  +    ++ +   L+L+   +   TL G +   +   G  +W      PIFSSP C   + +     G L   C   S E VW F + GPIFSS   S   S+I  G+H C V C +    L  W+ + T  V+ATP +  S       +   +STDG+I +       K  +    ++L G  FSSP+     I    ++    C
Sbjct:  193 QKHSIAYILHTSGTTGLPKIVKVPHKCIVPNIVHLRDVFKITP----DDTVFLASPLTFDPSVIELFIALSSGASLLIVPQQIKMMPQKLSEVLFQRHSVSVVQATPTLLRRFGVQCIKS-TVLSAHSSLRILALGGEAFPGLNVLRSWKEKGNRTHIFNIYGITEVSCWATCYKVPEEFFSADHRFDLMVPLGTPLLGTTVEVKDANGSAILEGEGQVFIGGKERVCFLD------------DEVTLPMGTMRATGDFVRVKDTEMFFLGRKDNQIKRHGKRLDIQQVQQIVEDHSEVESCAV-IWYQEKKLILFVVPKGILERRHFLNNLQKCLPGHAVPDELLLIEILPFTSHGKIDVSELNKVYSDHLSSRRNKRKLSRKELWEGLQYLWKSLLNLPDDSSSILKDSLFLHSGGDSLKSLQFQEEIEYMVGR-AVPSLLEVILSRSFEEVYQHVLKTVFPSKGLQLSCHGAVKRKLSESSTEDCSAKCVEPKSERSLSAKPDPVGFMAVNRGNRLFSVDALLKNQSNPEQGEDSAILKSMHDVTATSSAYVTELDSFGTPPVQENLGRTAEKWTLNIRWRSDLGKCVDASPLVVISTADKLSAFVYIGSHSHIIQAIDLYSGKVKWMRTLRDRIESSACISKCGNFIIVGCYHGFIYVLRSSDGEVHWTFKTEDAVKSSPAVDHSTGLVFIGSHDQHVYALDIYKEECVWKLHSEAGAVFSSPHLSLLPYQLYIGTLGGLLLAINPVTGNRIWKHFCGKPIFSSPHCDKNYVFVGCVDGNL--YCFNHSGEKVWQFSSNGPIFSSPCVSSCASEIFFGSHDCCVYCCNKEGQLV-WKFETTSAVYATPFVFHSHDSEDKTLLAVVSTDGRIWILN----TKTGFVEGTEKLPGEVFSSPVVWGSAIVVGCRNNFVYC 1087