BLAST of sodium-potassium-chloride cotransporter vs. L. salmonis genes
Match: EMLSAG00000003059 (supercontig:LSalAtl2s:LSalAtl2s1734:14347:19068:1 gene:EMLSAG00000003059 transcript:EMLSAT00000003059 description:"maker-LSalAtl2s1734-augustus-gene-0.5")

HSP 1 Score: 574.704 bits (1480), Expect = 0.000e+0
Identity = 316/755 (41.85%), Postives = 459/755 (60.79%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNN----CTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDE--DDDEDLDTGMTL-------KISSILGDKLKKSRQRHRSKR-----RSVFTGQNGVAVP------------QVILDDLNVFKQKKKL--GVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDI-------SSKAG---------------------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP VAIPKG                    TL  ++ TYV+Y  Y +MI +    EASGD  E + ++N+    EV +  N    C  R C YG  + QQ +  IS    L+YAGCFAATLSSAIASLVGAPRV QA+AKDKL+P I  F+ G GKNN+P +GY+L F I+L C+++G+L+ VSSLLSNFFVAAYALINFSVFHAS+ KSPGWRP F+YYN+W+SL GT LC+ VMFLMD +TA +T  +V  LY Y+  RKPE +WGSST+AQ FM  L +V  L+   DHVKNYRPKIMV SG+P +R  L+DFA+L+TK++SLL   D + ED+  K+  +IK     W  ++ +K F  V       +GA + M L+GLGK   NMIL+GF+  W   +   + YY I+  A D+ L++ +LR+  G D +       ++E + + ++ + L YD+  + +  +D+G+ L         S+  G+  K+     R+K+     + +F  +   ++P            + I++ L+ F+ K  +  G IDV+WLYDDG        IL+ R +++ C++RVF L +  D+LD +T++M SLL KFRID++DV+++ D T +  + T+ +F  +       +S +G                     ++   +I N+E++N  LR++E +K+ S+++ ++VMTLPMP+  ++   +YM+WL+  TKD+  PFL VRGNQ+SVLTFYS
Sbjct:  408 LSGDLKDPGVAIPKG--------------------TLLXIVSTYVTYTIYGVMIAAXTXREASGDVLELD-YVNETIK-EVNNITNSFWECENRTCSYGILHSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVFQAVAKDKLFPGIASFADGWGKNNDPVKGYLLVFLISLICILIGDLNYVSSLLSNFFVAAYALINFSVFHASITKSPGWRPGFKYYNKWISLLGTVLCICVMFLMDMVTALVTFIIVFFLYMYVSIRKPEVNWGSSTQAQSFMTALNSVLSLTKVEDHVKNYRPKIMVMSGSPLNRPTLIDFANLVTKRISLLESVDVVPEDLDWKKSQAIKNSGQNWFTENHIKGFFSVVRNSKISEGARTAMELSGLGKLRTNMILLGFQDTWWKKDESAKEYYKIIQTAFDLRLAVGVLRINGGLDVTSMTN---LME-DSSPNQSNALNYDDRCESNMSVDSGLDLATKSGAKNTSTQSGEDDKEDGSLKRNKKPNGFEKLLFKKKCPSSIPLPPSEKDVKPLDEDIVNRLSQFRDKTTVNTGTIDVYWLYDDGXXXXH---ILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVPPLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLIVMTLPMPKKEIVPQELYMAWLDYTTKDM-PPFLLVRGNQTSVLTFYS 1132          

BLAST of sodium-potassium-chloride cotransporter vs. L. salmonis genes
Match: EMLSAG00000009267 (supercontig:LSalAtl2s:LSalAtl2s58:680408:685072:1 gene:EMLSAG00000009267 transcript:EMLSAT00000009267 description:"augustus_masked-LSalAtl2s58-processed-gene-6.1")

HSP 1 Score: 408.683 bits (1049), Expect = 8.124e-131
Identity = 204/425 (48.00%), Postives = 289/425 (68.00%), Query Frame = 0

Query:  273 EAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIR-EDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDI--SSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            + QQF+ TL+++  L+ TP+HVKNYRPK++VF+G P+HR PL+DFA+L+TKKLSLLMCA +   D   K  + ++     W   HK+K+F+ +   KNFE+GA++CMNLAG+GK  PNM+L G+K DWL+D  G + Y ++LH A D++L + ILRL  GCDFS+ +  E   + E+  DEE++      D+++   G +L+        L++ + R R    +V+TG +G  +P+  + D+  F+ KK+ G IDVWWLYDDGGLTLLLP IL  R QFA CQLRVFAL +  D+LD ET+NM  LL KFRI Y  V +IPDVT +A + T+ +F  I    K  +    +I  +E++NR+LRL+E L+E+S  S+M++MTL +PR     + +YMSWLE+MTK++  PFL +RGNQSSVLTFYS
Sbjct:  537 KXQQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHVNIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLAGIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKVIGEEEQQQHEIELDEENE------DEKNGKQGKSLE--------LQQKKVRXRKVSTAVYTGIDGNPLPKNTVQDITQFQIKKRKGNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKILDEIKPPLEESEIISQKEKTNRHLRLTELLREHSKRSEMIIMTLSIPRRGTAVAQLYMSWLEMMTKNM-PPFLLIRGNQSSVLTFYS 946          

HSP 2 Score: 196.438 bits (498), Expect = 4.436e-53
Identity = 96/182 (52.75%), Postives = 122/182 (67.03%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRK----CEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMV 178
            +SGDLKDP VAIPKG                    TL A+I+T+++Y+GY  +I     +EASG+  EY  ++N         FNNC+ R     C+YG+SNDQQ + +ISYT YL+YAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFI +F+ G G NN+P RGY L F I++ C+++
Sbjct:  364 LSGDLKDPGVAIPKG--------------------TLLAIIVTFITYIGYGTIITGVMHTEASGNQTEYENWIN--GVEGALYFNNCSGRPADDFCQYGSSNDQQAMERISYTGYLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIHIFAPGFGANNDPIRGYFLVFIISMGCILI 523          

BLAST of sodium-potassium-chloride cotransporter vs. L. salmonis genes
Match: EMLSAG00000007778 (supercontig:LSalAtl2s:LSalAtl2s455:327857:377250:-1 gene:EMLSAG00000007778 transcript:EMLSAT00000007778 description:"maker-LSalAtl2s455-augustus-gene-2.20")

HSP 1 Score: 167.162 bits (422), Expect = 3.259e-43
Identity = 91/299 (30.43%), Postives = 143/299 (47.83%), Query Frame = 0

Query:  112 LVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSH----RVPLMDFAHLLTKKLSLLMCADIREDVTIK---EKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDE 403
            +V  G   +T+ + + SL GAPR+LQA+AKD + PF+  F     K  EP R   ++  I    V++GN+D ++ LLS FF+  Y  +N +    +++++P WRP F+YY+  +SL G  LC+AVMF+     A + + + GI+Y YI YR  E  WG             ++  L   P H KN+RP+I++ +   S        L   A  L     L +   + +    K   E  + K    + +    +K F  V   K+  DG        GLG   PN +++G+   W   E
Sbjct:  535 VVEIGSLLSTIGAGLQSLTGAPRLLQAIAKDGIIPFLAPFQVQSAKG-EPVRALGITLLICEVAVLIGNVDEIAPLLSMFFLMCYGFVNLAXALQTLLRTPNWRPRFKYYHWSLSLLGVVLCIAVMFMSSWYYALLAMGIAGIIYKYIEYRGAEKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILAKLDSQLKVKHKRLFALASQLKAGKGLTIGVTVLDGHFGKMYGEAQAAKFSIQQVIKDEAVKGFADVVVSKDISDGVCHLTQTTGLGGMKPNTVILGWPNSWRKKE 832          

HSP 2 Score: 62.7734 bits (151), Expect = 2.352e-10
Identity = 55/204 (26.96%), Postives = 97/204 (47.55%), Query Frame = 0

Query:  520 KKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSKAGITTDMLIENQERS--NRYLRLSESLKEYSTNSQMVVMT-------------------LP-----MPRPSMISSGI---YMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            K  G ID+WW+  DGGL +LLP +LK  + + NC+LR+F++    D+     K++ + L   RI+  DV ++    ++ SE T    L +  +  +  +M + ++ +    R L L++ + EY     +++ T                   +P     +P     S  I   +M +LE++T+ L+   L VRG    V+T YS
Sbjct:  868 KVKGNIDIWWIVHDGGLLMLLPFLLKQHRTWKNCKLRIFSVAQHEDNSIQMKKDLKTFLYHLRIE-ADVEVVEMQDSDISEYTYERTLLMEQRNEMLKEMRVSDKRKGKMTRRLSLNKGVGEYKVVDDIIIHTDKVTNEIEVETVIENSRADVPNIEDKIPPKVKFSEEIDEVHMEFLEVLTEGLER-VLMVRGGGREVITIYS 1069          

BLAST of sodium-potassium-chloride cotransporter vs. L. salmonis genes
Match: EMLSAG00000003697 (supercontig:LSalAtl2s:LSalAtl2s1:1949199:1954526:1 gene:EMLSAG00000003697 transcript:EMLSAT00000003697 description:"maker-LSalAtl2s1-augustus-gene-19.17")

HSP 1 Score: 122.479 bits (306), Expect = 5.051e-29
Identity = 97/409 (23.72%), Postives = 178/409 (43.52%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMD-TMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIRE-DVTIKEKDSIKADANEW--LVKH-KMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEM 404
            +SGDLK+P+ +IPKG                    TL A   T+++YV    +                 A   D   L          R C Y        + + S+    V  G   AT  ++ + L+G+ RVL+A++KD ++     + +     N P    ++++ ++     +G ++ ++  ++  F+ +YA +N S     +  +P +RP FR ++ +    G    + +MF+++  ++A +  A +            + +WGS ++A  F    + +  L    DHVK +RP++++   NP +   L+DFA+ L KK  L +   +RE +    E+D        W  LV H K+KAF  +        G    + ++G+G   PN I+ GF  +  D+ M
Sbjct:  267 LSGDLKEPSKSIPKG--------------------TLLASFFTFITYVILFTL----------------TAMTCDKELL---------LRDCSY--------MIEFSFWKPFVIIGTVLATFCASTSCLIGSSRVLEAISKDTMFGIFLEWITYGTVGNNPVLAVVVTWLLSNIFFFIGGVNTIAQFVAVLFLLSYASVNLSCLSLDLASAPNFRPIFRCFSWYTCALGLIGTLTMMFMINPVISALVLFACIFFFLSLSFLSPTKENWGSISQALLFHQVRKYLLLLDSRKDHVKFWRPQVLLLVSNPRNHCSLIDFANAL-KKGGLYVLGHVREGNFEGMEEDECGLLMPHWLNLVDHLKVKAFVELTVAPTVRSGIQQLIRISGIGAMKPNTIIFGFPYE--DENM 619          

BLAST of sodium-potassium-chloride cotransporter vs. L. salmonis genes
Match: EMLSAG00000004234 (supercontig:LSalAtl2s:LSalAtl2s222:1210791:1213007:1 gene:EMLSAG00000004234 transcript:EMLSAT00000004234 description:"maker-LSalAtl2s222-snap-gene-12.7")

HSP 1 Score: 119.013 bits (297), Expect = 3.197e-28
Identity = 80/293 (27.30%), Postives = 144/293 (49.15%), Query Frame = 0

Query:  113 VYAGCFAATLSSAIASLVGAPRVLQALAKDKLY-PFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAH--WGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIRED-VTIKEKDSIKADANEWLVK---HKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTD 398
            V  G F AT S+++ +L+G  R+L+A+AKD ++ P       G  K N P    I ++ +    +M+G+L+ ++ L S  F+ +Y  IN +     +  +P +RP ++Y++ +  L G    + +MF +  + + + I +   L   +    P  H  WGS ++A  F    + +  L     HVK +RP++++   NP     L++F + L KK  L +   +  D +   +KD    +   WL      K+KAF  V   ++   G    + ++G+G   PN +L+GFK D
Sbjct:  283 VTIGVFMATFSASLNNLIGXSRILEAIAKDVVFGPLFNFVLKGTYKEN-PVTAVISTWFVVELFLMMGSLNNIAQLCSVLFLLSYGTINLTCLFLHLASAPNFRPTYKYFSTYTCLIGLLGSIIMMFAVSAIYSAVGILLCLSLILLLNLFSPIRHTNWGSISQALIFHQVRKYLLLLDQRKSHVKFWRPQMLLLVSNPRRNCSLIEFGNAL-KKSGLYVLGHVHVDNLEEHDKDPTLNEIPHWLKLIDFLKVKAFVEVTIARSLRLGVDQLVRISGIGAMKPNTVLLGFKDD 573          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|1709292|sp|P55011.1|S12A2_HUMAN (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1)

HSP 1 Score: 531.561 bits (1368), Expect = 1.149e-173
Identity = 288/740 (38.92%), Postives = 432/740 (58.38%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEV-----------FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIR---EDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEM-GTERYYNILHQALDMNLSLTILRLPRGCDFSQ-HVQHECIVEVEVTNDEEDDLCYDE-DDDEDLDTGMTLK---ISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVP-----QVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF---------------LDISSKAG------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL DP  AIPKG                    TL A+++T + YVG A+ +GSC + +A+G+       +ND    E+           F F++C    C YG  N+ Q +S +S  + L+ AG F+ATLSSA+ASLV AP++ QAL KD +YP   +F+ G+GKNNEP RGYIL+F IAL  +++  L+V++ ++SNFF+A+YALINFSVFHAS+ KSPGWRPAF+YYN W+SL G  LC  VMF+++   A +T  +V  LY Y+ Y+KP+ +WGSST+A  ++N L++   LS   DHVKN+RP+ +V +G P+ R  L+   H  TK + L++C  +        +KE    +A    WL+K+KMKAF+   +  +  +GA   M  AGLG+  PN +++GFK DWL  +M   + Y N+ H A D+   + ++RL  G D S    Q E +   E +   +D +   E     DLDT   L    I+  + ++  K+  +   K+ S      G  VP     Q +L+    F++K+    IDVWWL+DDGGLTLL+P +L  +K++ +C++RVF +G  ++ +D + + M +LL KFRID+ D++++ D+ T+  +     F                DI+ K        IT + L   + ++ R +RL+E LKE+S+ + ++VM+LP+ R   +SS +YM+WLE ++KDL  P L VRGN  SVLTFYS
Sbjct:  507 ISGDLADPQSAIPKG--------------------TLLAILITTLVYVGIAVSVGSCVVRDATGN-------VNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKES-----KGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDL-PPILLVRGNHQSVLTFYS 1212          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|341941994|sp|P55012.2|S12A2_MOUSE (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1)

HSP 1 Score: 530.02 bits (1364), Expect = 4.183e-173
Identity = 285/740 (38.51%), Postives = 433/740 (58.51%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEV-----------FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIR---EDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEM-GTERYYNILHQALDMNLSLTILRLPRGCDFSQ-HVQHECIVEVEVTNDEED---DLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQN-GVAVP-----QVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF---------------LDISSKAG------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL DP  AIPKG                    TL A+++T V Y+G A+ +GSC + +A+G+       +ND  T E+           F F+ C    C YG  N+ Q +S +S  + L+ AG F+ATLSSA+ASLV AP++ QAL KD +YP   +F+ G+GKNNEP RGYIL+F IAL  +++  L+V++ ++SNFF+A+YALINFSVFHAS+ KSPGWRPAF+YYN W+SL G  LC  VMF+++   A +T  +V  LY Y+ Y+KP+ +WGSST+A  +++ L++   LS   DHVKN+RP+ +V +G+P+ R  L+   H  TK + L++C  +        +KE    +A    WL+K+KMKAF+   +  +  +GA   M  AGLG+  PN +++GFK DWL  +M   + Y N+ H A D+   + ++RL  G D S    Q E +   E +   +D   ++ Y +  D+D     T K S       K   +  ++  + +   ++ G  VP     Q +L+    F++K+    IDVWWL+DDGGLTLL+P +L  +K++ +C++RVF +G  ++ +D + + M +LL KFRID+ D++++ D+ T+  +     F                DI+ K        IT + L   + ++ R +RL+E LKE+S+ + ++VM+LP+ R   +SS +YM+WLE ++KDL  P L VRGN  SVLTFYS
Sbjct:  500 ISGDLADPQSAIPKG--------------------TLLAILITTVVYIGIAVSVGSCVVRDATGN-------VNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVNVDYSKKSDQD-----TCKSSGEKSITQKDEEEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMSLPVARKGAVSSALYMAWLEALSKDL-PPVLLVRGNHQSVLTFYS 1205          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|1709294|sp|P55013.1|S12A2_SQUAC (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; AltName: Full=NKCC; AltName: Full=Na-K-CL symporter)

HSP 1 Score: 519.235 bits (1336), Expect = 3.632e-169
Identity = 281/735 (38.23%), Postives = 425/735 (57.82%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASG---DAAEYNAFLN-DNATLEV-FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIR---EDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMG-TERYYNILHQALDMNLSLTILRLPRGCDFSQ-HVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKIS-----SILGDKLKKSRQRHRSKRRSVFTGQNGVAVP-----QVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDI---------------SSKAG------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL DP +AIPKG                    TL A+++T + Y G A+ +GSC + EA+G   DA       N  N   ++ F+F++C   KC YG  ND Q +S +S    L+ AG F+ATLSSA+ASLV AP++ QAL KD +YP + VFS G+GKNNEP RGY+L+F I L  +++  L+V++ ++SNFF+A+YALINFSVFHAS+ KSPGWRPAFR+YN W+SL G  LC  VMF+++   A +T  +V  LY Y+ Y+KP+ +WGSST+A  ++N L++   L+   DHVKN+RP+ ++ +G P+ R  L+   H  TK + L++C  +        +KE  + +A    WL+K+KMKAF+   Y ++  +G    +   GLG+  PN ++ GFK DW    M   E Y N +H A D    + ++RL  G + S    Q E     E +   +D +   E  D D     +  +S     ++  D+  +      S +  +       +VP     Q +L   + F++K+  G IDVWWL+DDGGLTLL+P +L  +K++ +C++RVF +G  ++ +D + + M +LL KFRID+ D+ ++ D+ T+ S+   T F ++               S K        IT + L   + ++ R +RL+E L+E S  + ++VM+LP+ R   +SS +YM+W+E ++KDL  P L VRGN  SVLTFYS
Sbjct:  479 ISGDLADPQLAIPKG--------------------TLLAILITTIVYAGAAVSVGSCIVREATGNLTDAIIPGTVTNCTNVACKLGFNFSSCATNKCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHVFSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFRFYNMWISLIGAILCCGVMFVINWWAALLTNVIVLALYIYVTYKKPDVNWGSSTQALTYLNALQHAIRLTGVEDHVKNFRPQCLLMTGAPTSRPALLHLVHAFTKNVGLVVCGHVHTGPRRQALKEISTDQAKYQRWLIKNKMKAFYAPVYAEDLREGTQFLLQAVGLGRMRPNTLVFGFKKDWRQALMKDVENYINAIHDAFDYQYGVVVIRLKEGFNISHLQAQEELCTSQEKSAHPKDIVVNLEHSDADSSKPSSKSVSETNSPAVCQDQKDEEDDGKASTQPLLKKEVKDPSVPLNMTDQKLLQASSQFQKKQGKGTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMIEPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLIVMSLPVARKGAVSSALYMAWIETLSKDL-PPILLVRGNHQSVLTFYS 1191          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|1709297|sp|P55016.1|S12A1_RAT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 503.442 bits (1295), Expect = 5.139e-164
Identity = 276/730 (37.81%), Postives = 423/730 (57.95%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASG---DAAEYNAFLNDNATLEV-FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI---REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGT-ERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYD---EDDDEDLDTGMTLKISSILGD----KLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEA---------------------TQTDFLDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL+DP  AIP+G                    T+ A+ +T V+Y+G AI + +C + +A+G   D        N +A   + + F+ C    C+YG  N+ Q +S +S    L+ AG F+ATLSSA+ASLV AP+V QAL KD ++  +  F+ G+GKNNEP RGY L+F IA+A +++  L+V++ ++SNFF+A+YALINFS FHAS  KSPGWRPA+  YN WVSL G  LC AVMF+++   A IT  +   LY Y+ Y+KP+ +WGSST+A  +++ L+N  +L+   DHVKN+RP+ +V +G P  R  L+D  H  TK   L +C ++      + +KE +S  A    WL+K+K+KAF+       F DG  S +  +GLG+  PN +++G+K +W    +   E Y  I+H A D  + + I+R+ +G D S  +Q    V+ E+   E++ L  +   +D+D +   G    +    G     K    +  + S  +S+  G+      Q +++    FK+K+  G IDVWWL+DDGGLTLL+P IL +RK++ +C+LR++ +G  ++ ++ E  +M SLL KFRI + D+ II D+  + ++                      T  + L   S   IT   L   +E+S R +RL+E L+E+S  + ++V++LP+ R   IS  +YM+WLEI+TK+L  P L VRGN  +VLTFYS
Sbjct:  396 ISGDLEDPQDAIPRG--------------------TMLAIFITTVAYIGVAICVRACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQ----VQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLNITKPAPKKDSNISTIQSMHVGE----FNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNL-PPVLLVRGNHKNVLTFYS 1095          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|27151793|sp|P55014.2|S12A1_MOUSE (RecName: Full=Solute carrier family 12 member 1; AltName: Full=BSC1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 502.671 bits (1293), Expect = 8.569e-164
Identity = 274/730 (37.53%), Postives = 421/730 (57.67%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASG---DAAEYNAFLNDNATLEV-FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI---REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGT-ERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDE-------DDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEA---------------------TQTDFLDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL+DP  AIP+G                    T+ A+ +T V+Y+G AI + +C + +A+G   D        N +A   + + F+ C    C+YG  N+ Q +S +S    L+ AG F+ATLSSA+ASLV AP+V QAL KD ++  +  F+ G+GKNNEP RGY L+F IA+A +++  L+V++ ++SNFF+A+YALINFS FHAS  KSPGWRPA+  YN WVSL G  LC AVMF+++   A IT  +   LY Y+ Y+KP+ +WGSST+A  +++ L+N  +L+   DHVKN+RP+ +V +G P  R  L+D  H  TK   L +C ++      + +KE +S  A    WL+K+K+KAF+       F DG  S +  +GLG+  PN +++G+K +W    +   E Y  I+H A D  + + I+R+ +G D S  +Q    V+ E+   E++ L  +        ++ +    G+  K   +   K    +  + S  +S+  G+      Q +++    FK+K+  G IDVWWL+DDGGLTLL+P IL +RK++ +C+LR++ +G  +  ++ E  +M SLL KFRI + D+ II D+  + ++                      T  + L   S   IT   L   +E+S R +RL+E L+E+S  + ++V++LP+ R   IS  +YM+WLEI+TK+L  P L VRGN  +VLTFYS
Sbjct:  396 ISGDLEDPQDAIPRG--------------------TMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQ----VQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPKKDGNISSIQSMHVGE----FNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIKPNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNL-PPVLLVRGNHKNVLTFYS 1095          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|212276464|sp|Q13621.2|S12A1_HUMAN (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 499.204 bits (1284), Expect = 2.049e-162
Identity = 274/731 (37.48%), Postives = 422/731 (57.73%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAE-YNAFLNDNATLEV---FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI---REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMG-TERYYNILHQALDMNLSLTILRLPRGCDFSQHVQ--------HECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEA---------------------TQTDFLDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL+DP  AIP+G                    T+ A+ +T V+Y+G AI +G+C + +A+G+  +   + +N N +      + F+ C    C+YG  N+ Q +S +S    L+ AG F+ATLSSA+ASLV AP+V QAL KD +Y  +  F+ G+GKNNEP RGYIL+F IA+A +++  L+ ++ ++SNFF+A+YALINFS FHAS  KSPGWRPA+  YN WVSL G  LC AVMF+++   A IT  +   LY Y+  +KP+ +WGSST+A  +++ L+N  +L+   DHVKN+RP+ +V +G P  R  L+D  H  TK   L +C ++      + +KE +S  A    WL+K+K+KAF+       F DG  S +  +GLG+  PN +++G+K +W    +   E Y  I+H A D  + + I+R+ +G D SQ +Q         +  + +E T   +D+ C +E        G+  K   +   K    +    +  +S+  G+      Q +++    FK+K++ G IDVWWL+DDGGLTLL+P IL +RK++ +C+LR++ +G  ++ ++ E   M SLL KFRI + D+ II D+    ++                      T  + L   +   IT   L   +E+S R +RL+E L+E+S  + ++V++LP+ R   IS  +YM+WLEI+TK+L  P L VRGN  +VLTFYS
Sbjct:  400 ISGDLEDPQDAIPRG--------------------TMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEAT--IKDNECEEESGG---IRGLFKKAGKLNITKTTPKKDGSINTSQSMHVGE----FNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIRPNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNL-PPVLLVRGNHKNVLTFYS 1099          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|1709296|sp|P55015.1|S12A1_RABIT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 497.664 bits (1280), Expect = 9.586e-162
Identity = 269/723 (37.21%), Postives = 416/723 (57.54%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASG---DAAEYNAFLNDNATLEV-FSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI---REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMG-TERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEA---------------------TQTDFLDISSKAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL+DP  AIP+G                    T+ A+ +T V+Y+G AI +G+C + +A+G   D        N +A   + + F+ C    C+YG  N+ Q +S +S    L+ AG F+ATLSSA+ASLV A +V QAL KD +Y  +  F+ G+GKNNEP RGYIL+F IA+A +++  L+ ++ ++SNFF+A+YALINFS FHAS  KSPGWRPA+  YN WVSL G  LC AVMF+++   A IT  +   LY Y+ Y+KP+ +WGSST+A  +++ L+N  +L+   DHVKN+RP+  V +G P  R  L+D  +  TK   L +C ++      + +KE +S  A    WL+K+K+KAF+       F DG  S +  +GLG+  PN +++G+K  W    +   E Y  I+H A D  + + I+R+ +G D SQ +Q +  +E         +    +++ E+ + G+      +    + K   +  S   ++ +   G    Q +++    FK+K+  G IDVWWL+DDGGLTLL+P IL +RK++ +C+LR++ +G  ++ ++ E   M SLL KFRI + D+ +I D+  + ++                      T  + L   +   IT   L   +E+S R +RL+E L+E+S  + ++V++LP+ R   IS  +YM+WLEI+TK+L  P L VRGN  +VLTFYS
Sbjct:  400 ISGDLEDPQDAIPRG--------------------TMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGINCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAAKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCFVLTGGPMTRPALLDITYAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKKWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELEKLEQERLALEATIKDNECEEGNGGIRGLFKKVGKLNITKPTPKKDSSINTIQSMHVG-EFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINIKPNKESWKFFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDLLYMAWLEILTKNLP-PVLLVRGNHKNVLTFYS 1099          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|2500792|sp|Q25479.1|NKCL_MANSE (RecName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter; AltName: Full=Na-K-CL symporter)

HSP 1 Score: 490.345 bits (1261), Expect = 2.109e-159
Identity = 271/736 (36.82%), Postives = 432/736 (58.70%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEY---NAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI-REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMG-TERYYNILHQALDMNLSLTILRLPRGCDF----SQHVQHECIVEVEVTND------EEDDLCYDEDDDEDL-DTGMTLKISSILGDKLKKSRQRHRS-----------------KRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSK---AGITTDMLIENQE------RSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDPA AIPKG                    TL A++++ VSY    +  G   L +ASG+  +    N  + D +++ + + NN     CEYG  N    +  +S     +Y GC+AATLS+A+ +L+  PR++QAL  D++YP +  FS  +G++ EP+RGY+L+F ++L  +++ +L+ ++ L+SNF++A+YALINF  FH ++V+  GWRP FRYYN W+SLAG  +CVA+M L+  + + +T A+   LY  + YR+P+ +WGSST+AQ +   L + + L+ T +HVKNY P+++V +G P  R  L+D  +L++K  SL++  DI +E ++ K + +     +EWL   K++AF    +  +FE GA + +  +G+G+ APN++LMG+K+DW          Y+N+LH A +  L++ I+R+  G D+    S+  +      +  T+         D L    D D D+ DT     +S++L   L  SR    S                  R+ V+   +G+ + +  L  +++F++K++ G +DVWWLYDDGGLT+LLP I+  R  +ANC+LR+FAL + + +++ E +NM +LL KFRIDY  + ++ D+T      T+  F +   K      + D  I + E      ++NR LRL E L   S ++++VVM+LPMPR   IS+ +YM+WLE+M++DL  P LFVRGN +SVLTFYS
Sbjct:  352 ISGDLKDPASAIPKG--------------------TLLALLISMVSYTLMVLFAGGGALRDASGNITDLLIVNGTVTDYSSVSLCALNNT----CEYGLHNSYSVMQLMSAWGPFIYGGCWAATLSTALTNLLSVPRLIQALGVDRIYPGLIFFSKPYGRHGEPYRGYVLTFFVSLLFLLIADLNTIAPLISNFYLASYALINFCTFHRALVRPLGWRPTFRYYNMWLSLAGFLMCVAIMLLVHWVMSLVTFAIFFTLYLIVHYRRPDVNWGSSTQAQMYKTALSSAHALARTGEHVKNYWPQLLVLAGRPQARPALVDLGNLISKAGSLMIVGDISQEKLSYKVRSARARSDDEWLRGRKVRAFCSRVHGFSFEPGARALVQGSGVGRLAPNVLLMGYKSDWTTCPANDLVSYFNVLHTAFENRLAVAIVRVSGGLDYSAVVSEGAEEGAAGSLTATSSSGELRVRRDGLIMHADSDLDIRDTQPKHNLSNLL--TLTTSRSFTISECKEKDKKKKERKPNDMHRQIVYNTASGLELSKFQLAQMSLFQKKQESGTLDVWWLYDDGGLTILLPYIISQRSAWANCKLRIFALANRLHEMELEERNMANLLAKFRIDYSSLTMVQDITDPPQPETKALFDETIKKFTEESASPDCRISDMELQTLAVKTNRQLRLRELLLANSKDARLVVMSLPMPRKGSISAPLYMAWLEMMSRDLP-PMLFVRGNHTSVLTFYS 1060          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|313104194|sp|P55017.3|S12A3_HUMAN (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl cotransporter; Short=NCC; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter)

HSP 1 Score: 442.58 bits (1137), Expect = 1.553e-141
Identity = 256/730 (35.07%), Postives = 393/730 (53.84%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEV-----------FSFNNCTFR-KCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIREDVTIKEKDSIKADAN---EWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGT-ERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISS---------------------KAGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFY 693
            +SGDLKDPA+AIPKG                    TL A+  T +SY+  +  IGSC + +ASG        LND  T              ++F  CT +  C YG  N  QT+S +S  + L+ AG F ATLSSA+A LV A +V Q L +D+LYP IG F  G+GKN EP RGY+L++ IA+A +++  L+ ++ ++SNFF+ +YALINFS FHAS+  SPGWRP+F+YYN+W +L G  + V +MFL+    A I I VV  L  Y+ Y+KPE +WGSS +A  +   L     L+   DH+KNYRP+ +V +G P+ R  L+DF    T+ LSL++C  +      +    ++  AN   +WL K K+KAF+     ++   G    M  AGLG+  PN++++GFK +W      T E Y  ILH A D N  + ++R+  G + S+ +Q      +    D  +D    ++    +D    +K                  +  ++F  + G                KK    ID++WL+DDGGLTLL+P +L  +++++ C++RVF +G  ++ +D E K + SLL KFR+ + +V I+PD+           F D+ +                        I+ + + +N+ +S R +RL+E + +YS ++ ++V+TLP+ R     S +YM+WLE +++DL  P + +RGNQ +VLTFY
Sbjct:  360 ISGDLKDPAIAIPKG--------------------TLMAIFWTTISYLAISATIGSCVVRDASG-------VLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQ----AHINPVFDPAED---GKEASARVDPKALVK----------------EEQATTIFQSEQG----------------KK---TIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVF-VGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFY 1019          

BLAST of sodium-potassium-chloride cotransporter vs. SwissProt
Match: gi|1717802|sp|P55019.1|S12A3_PSEAM (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter)

HSP 1 Score: 430.639 bits (1106), Expect = 7.587e-137
Identity = 252/727 (34.66%), Postives = 385/727 (52.96%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFR----------KCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDW-LDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSKAGITTD------------------MLIE-----NQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFY 693
            +SGDLKDP VAIP+G                    TL A+  T +SY+  +  IG+C + +ASG   E N  L+ +++ E  S   C +R           C++G  N  Q++S +S  + L+ AG F ATLSSA+A LV AP+V Q L KD+LYP IG F  G+GKN EP R Y+L++ IA+  V++  L+ ++ ++SNFF+ +YALINFS FHAS+  SPGWRP+FR+Y++W+SL G   CV +MFL+    A I   VV  L  Y  Y+KP  +WGSS +A  +   L     L+   DHVKNYRP+ +V +G P  R  L+D    LTK+LSL+MC      V       +      WL + K+++F+      +   G    +  AGLG+  PN++LMGFK DW  D       Y  ILH A D++  + +LR+  G D S   Q       +   +  + +C        + + +++                                 PQ      +VF++K+    ID++WL DDGGLTLLLP +L  RK++A C++RVF  G T D  + + + + +L++KFR+ + DV ++PD+           F D  ++  + T+                  M+ E     N+ +S R +RL+E L+ +S  + ++V+T+P+ R  +  S ++++WL+++++DL  P L VRGNQ +VLTFY
Sbjct:  357 ISGDLKDPTVAIPRG--------------------TLMAIFWTTISYLIISATIGACVVRDASG---ELNDTLSYSSSSENCSGLACQYRWDFSECIKNNTCKHGIMNYYQSMSLVSAFAPLISAGIFGATLSSALACLVSAPKVFQCLCKDQLYPLIGFFGKGYGKNAEPLRAYLLTYVIAVCFVLIAELNTIAPIISNFFLCSYALINFSCFHASVTNSPGWRPSFRFYSKWLSLLGAVCCVVIMFLLTWWAALIAFGVVFFLLGYTLYKKPAVNWGSSVQASSYSMALNQCVGLNQVEDHVKNYRPQCLVLTGPPCCRPALVDLVGCLTKRLSLMMCG----HVVTAGPSPVSERHVTWLNQRKVRSFYRGVVAADLRSGVNMLLQGAGLGRIKPNVLLMGFKKDWGCDSPQAAHHYIGILHDAFDLHYGVCVLRVKEGLDASHPPQCHVNPGFDGGPESINTVCAPACVQTSVTSSVSMDPD------------------------------PQ----PSSVFQKKQGKKTIDIYWLSDDGGLTLLLPYLLTRRKRWAGCKVRVFVGGDT-DKKEEQKEEVLALIKKFRLGFHDVEVLPDIHQPPQPGNVDHFEDSVNRFRLETNPKQDSDSGPQQQQQEEPWMITEQDLERNRAKSLRQIRLNEVLQVHSREAALIVITMPVGRRGVCPSTLFLAWLDVLSRDLRPPVLLVRGNQENVLTFY 1021          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|242016228|ref|XP_002428731.1| (sodium-potassium-chloride cotransporter, putative [Pediculus humanus corporis] >gi|212513416|gb|EEB15993.1| sodium-potassium-chloride cotransporter, putative [Pediculus humanus corporis])

HSP 1 Score: 628.246 bits (1619), Expect = 0.000e+0
Identity = 331/713 (46.42%), Postives = 461/713 (64.66%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI-REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDW----LDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSKAGITTDMLIEN--------------QERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP+ AIPKG                    TL A+ +T++SY+ YA M+G C L +ASGD  +Y   LN++  L      NCT   C YG  ND Q +   S    L+Y GCFAATLSSAIASLVGAPRVLQALAKDKLYPFIG FS G+G NN+P RGY+L FC + AC+++  L+ ++ LLSNFF+ AYALINFSVFHAS+ KSPGWRPAF+YYN WVSL GT LC+ VMFL+   TA IT  VV  LY Y+ YRKP+ +WGSST+AQ +   L +V DL+   +HVKNYRP+I++ SG P  R PL+ F +L+ K LSLL+ + I  E+V+ +E D++    + WL  HK+K F  V   KNFE GA S M   G+GK  PNM+LMG+K+ W    LD+ +   +Y+N++H ALD +L++ ILR+P G D+S  +Q E +V  E   + +      +  D +  +     +SS+L  + K +    + +R+ +    +G  V + +LD L  F++++  G IDVWWLYDDGGLTLL+P IL  R  F+ C+LRVF+L S  D+LD E + + +LL KFRI  +DV++IPD+  +A  +++ +F  +     +  D + E               +E+SNR++RL E L+E+S+++  +VMTLP+PR  ++S+ +YM+WLE++TK +  P+L VRGNQ+SVLTFYS
Sbjct:  382 LSGDLKDPSSAIPKG--------------------TLLAIFVTFLSYLAYAFMVGGCVLRDASGDVEDYIKGLNESNPL--LYVTNCTIGSCSYGLQNDSQAMELASLWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSEGYGANNDPVRGYVLVFCTSFACILIAQLNAIAPLLSNFFLTAYALINFSVFHASISKSPGWRPAFKYYNAWVSLLGTLLCILVMFLISWWTALITFVVVVTLYLYVSYRKPDVNWGSSTQAQCYSIALRSVIDLNYMEEHVKNYRPQIVILSGLPGWRPPLVHFGNLVVKGLSLLISSHIVPEEVSQREIDNLTKRGHRWLAHHKVKGFFNVVAAKNFESGARSLMQNVGVGKLRPNMLLMGYKSTWKKCSLDELL---QYFNVIHGALDRHLAIGILRMPSGLDYSNRIQDEDLVAPENERERKRRKRNKKLIDRNNSSN---HLSSVLRSRFKGTIVP-KVERQPL--DLDGNPVSKDVLDSLMHFRKRQTAGNIDVWWLYDDGGLTLLIPYILSTRSIFSECRLRVFSLCSNADELDQEQRKLAALLSKFRISCKDVIVIPDIMKKAQTSSRIEFDGLVQNFRVRDDQVTEENKSIVISDTEYHNLREKSNRHMRLRELLREHSSDAAFIVMTLPVPRKGLVSAPMYMAWLELLTKGM-PPYLLVRGNQNSVLTFYS 1062          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|6179898|gb|AAF05702.1|AF190129_1 (Na+/K+/2Cl- cotransporter [Callinectes sapidus])

HSP 1 Score: 625.55 bits (1612), Expect = 0.000e+0
Identity = 323/733 (44.07%), Postives = 470/733 (64.12%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTF--------RKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDE-MGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIV------EVEVTNDEED--------DLCYDEDDDEDLDTGMTLKI--SSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF------------LDISSKAGITTDM-LIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDPA AIPKG                    TL+A++ T+ +Y+ Y IMIG+  L +A+GD   Y  + N +   E  +F NC+         + C++G  N  Q +  +S    L+YAGCFAATLSSAIASLVGAPRVLQALAKDKLYP I +FS G G NN+P RGYIL F I+  C+M+G+L+VVS+LLSNFF+A+Y+LINFS FHAS++KSPGWRP+F+YYN W+S  G  LC+ VMFL+D +TA  T  ++  LY ++ YR P  +WGSST+AQ +++ L+   DL+   +HVKNYRP+I+V +G    R PL+DF++ +TK +SLL C   I+   T + ++S+   +  WL +H ++AF+ +      EDGA +   L GLGK  PN +++G+K +W   E    + Y+N LH+ALDM   + ILR+P+G D+SQ ++ E         E  +T   +D         L  D  D E      + ++  S+ + D   ++ ++ R+   ++F G  G  + + +L+++ +FK+K+K G IDVWWLYDDGGLTLL+P IL  R Q++ C+LRVFAL +  D+LD E ++M +LL KFRIDY DV++IPDV  +A+E+++ +F            +D  S   + ++  L+  +E++NR++RL E L E S +S +VVMTLPMPR + +S+ +YM+WLE +T+D+  PFL +RGNQ+SVLTFYS
Sbjct:  321 LSGDLKDPADAIPKG--------------------TLAAILTTFCTYIIYPIMIGAAVLRDATGDKDVYLMYQNHSID-ENPAFTNCSLTGSVDNGTQVCKFGLQNSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIFMFSKGTGANNDPVRGYILVFVISFVCIMIGDLNVVSTLLSNFFLASYSLINFSCFHASLIKSPGWRPSFKYYNLWISWLGGILCLIVMFLIDWITALATFLIIIALYLFVSYRNPNVNWGSSTQAQTYVSALKTALDLNTIEEHVKNYRPQILVLTGPVGSRPPLIDFSYSITKNISLLACGHVIQGPQTQRLRNSLTRQSYNWLTRHSIRAFYSLVEGSTLEDGARNLFQLVGLGKLRPNTVVLGYKANWRKCEPKELKAYFNTLHEALDMYFGVVILRVPQGLDYSQIIEDEDSPITMNGNEGAITQTTDDKPGQSSANQLTQDGTDSEASSPPGSPQVERSAAVVDANGENSKKRRTSLANLFRGPGGSELSKDVLNNITMFKRKQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSGCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKKAAESSRMEFDQLIEDFKTKDEVDKESDGLLISEAELLGQREKTNRHIRLRELLLENSRDSTLVVMTLPMPRKTSVSAPLYMAWLETLTRDM-PPFLLIRGNQTSVLTFYS 1031          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|676657122|gb|AIM43577.1| (Na+/K+/2Cl- cotransporter isoform 1, partial [Halocaridina rubra])

HSP 1 Score: 615.535 bits (1586), Expect = 0.000e+0
Identity = 328/737 (44.50%), Postives = 472/737 (64.04%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATL-EVFSFNNCTFRK---------CEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTND-------EE-------DDLCYDEDDDE--DLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF----LDISSKAG-----------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP+VAIP+G                    TL+A++ T+++Y+ Y IMI +  + +A+G+   Y  + ++ + + E  +F N +  K         CE+G  N  Q +  +S    L+YAGCFAATLSSAIASLVGAPRVLQALAKDKLYP I +FS G+G NN+P RGYIL F I+ AC+M+G+L++VS+LLSNFF+A+Y LIN S FHA+++KSPGWRP+F+YYN WVSL G  +C+AVMFL+D +TA IT   V  LY ++ YR P+ +WGSST+AQ ++  L+   DL    +HVKNYRP+++V SG+   R PL+DFA  +TK +SLL     I+   T + ++++   +  WL +HK++AF+ +    N EDG+ +   L GLGK  PNM+L+G+K +W   D    + Y++ LH+ALDM L + ILR+P+G D+SQ ++ E    V   ND       EE         L  D++  E        TL+ ++       +   + R+   +++ G  GV + + +L ++ +FK+K+K G IDVWWLYDDGGLTLL+P IL  R Q++NC+LRVFAL +  D+LD E ++M +LL KFRIDY DV++IPDV   A+E+++ +F     D  +K+            I    L+  +E++NR+LRL E L E S +S +VVMTLPMPR S +S+ +YM+WLE +T+D+  PFL +RGNQ+SVLTFYS
Sbjct:  352 LSGDLKDPSVAIPRG--------------------TLAAILTTFITYMIYPIMIAAGTVRDATGNETAYLQYKDNVSNIWENPTFTNESCYKGYGENDVPHCEFGLINSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIHIFSKGYGANNDPVRGYILVFVISFACIMIGDLNLVSTLLSNFFLASYCLINISCFHATLIKSPGWRPSFKYYNSWVSLIGGLMCLAVMFLIDWVTALITFVCVIGLYMFVSYRNPDVNWGSSTQAQTYVTALKATLDLISVEEHVKNYRPQLLVLSGSAGSRPPLLDFAQSITKNISLLSLGHVIQGPQTQRIRNALSRQSYNWLARHKIRAFYSLVEGNNLEDGSRNLFQLVGLGKLRPNMVLLGYKANWGKCDRQELKAYFDTLHEALDMYLGIAILRVPQGLDYSQIIEDEDTPVVMNGNDTSITANTEEMKRNQSAGQLSQDDNGSEASSPPGSPTLERATNNDPAADEKAIKRRASLANLYRGPGGVELSKDVLSNITMFKRKQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSNCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKRAAESSRMEFDKLIEDFQAKSSEEISKEDEGTLICEAELLGQREKTNRHLRLRELLLENSKDSSLVVMTLPMPRKSSVSAPLYMAWLETLTRDM-PPFLLIRGNQTSVLTFYS 1067          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|676657102|gb|AIM43576.1| (Na+/K+/2Cl- cotransporter isoform 1, partial [Halocaridina rubra])

HSP 1 Score: 615.15 bits (1585), Expect = 0.000e+0
Identity = 328/737 (44.50%), Postives = 472/737 (64.04%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATL-EVFSFNNCTFRK---------CEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTND-------EE-------DDLCYDEDDDEDLD--TGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF----LDISSKAG-----------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP+VAIP+G                    TL+A++ T+++Y+ Y IMI +  + +A+G+   Y  + ++ + + E  +F N +  K         CE+G  N  Q +  +S    L+YAGCFAATLSSAIASLVGAPRVLQALAKDKLYP I +FS G+G NN+P RGYIL F I+ AC+M+G+L++VS+LLSNFF+A+Y LIN S FHA+++KSPGWRP+F+YYN WVSL G  +C+AVMFL+D +TA IT   V  LY ++ YR P+ +WGSST+AQ ++  L+   DL    +HVKNYRP+++V SG+   R PL+DFA  +TK +SLL     I+   T + ++++   +  WL +HK++AF+ +    N EDG+ +   L GLGK  PNM+L+G+K +W   D    + Y++ LH+ALDM L + ILR+P+G D+SQ ++ E    V   ND       EE         L  D++  E        TL+ ++       +   + R+   +++ G  GV + + +L ++ +FK+K+K G IDVWWLYDDGGLTLL+P IL  R Q++NC+LRVFAL +  D+LD E ++M +LL KFRIDY DV++IPDV   A+E+++ +F     D  +K+            I    L+  +E++NR+LRL E L E S +S +VVMTLPMPR S +S+ +YM+WLE +T+D+  PFL +RGNQ+SVLTFYS
Sbjct:  352 LSGDLKDPSVAIPRG--------------------TLAAILTTFITYMIYPIMIAAGTVRDATGNETAYLQYKDNVSNIWENPTFTNESCYKGYGENDVPHCEFGLINSFQVMELMSAWGPLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPGIHIFSKGYGANNDPVRGYILVFVISFACIMIGDLNLVSTLLSNFFLASYCLINISCFHATLIKSPGWRPSFKYYNSWVSLIGGLMCLAVMFLIDWVTALITFVCVIGLYMFVSYRNPDVNWGSSTQAQTYVTALKATLDLISVEEHVKNYRPQLLVLSGSAGSRPPLLDFAQSITKNISLLSLGHVIQGPQTQRIRNALSRQSYNWLARHKIRAFYSLVEGNNLEDGSRNLFQLVGLGKLRPNMVLLGYKANWGKCDRQELKAYFDTLHEALDMYLGIAILRVPQGLDYSQIIEDEDTPVVMNGNDTSITANTEEMKRNQSAGQLSQDDNGSEASSPPGSPTLERATNNDPAADEKAIKRRASLANLYRGPGGVELSKDVLSNITMFKRKQKKGTIDVWWLYDDGGLTLLVPYILTTRSQWSNCKLRVFALANRKDELDMEQRSMANLLAKFRIDYSDVIVIPDVAKRAAESSRMEFDKLIEDFQAKSSEEISKEDEGTLICEAELLGQREKTNRHLRLRELLLENSKDSSLVVMTLPMPRKSSVSAPLYMAWLETLTRDM-PPFLLIRGNQTSVLTFYS 1067          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1325273257|ref|XP_023321269.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Eurytemora affinis])

HSP 1 Score: 612.068 bits (1577), Expect = 0.000e+0
Identity = 320/707 (45.26%), Postives = 449/707 (63.51%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFN----------NCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSK-AGITTDM--LIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP+ +IPKG                    TL A+I+TY+SY  +A+++G  +L +ASG+  EY    N     E+F+ +          N +   C+YG+++DQ+T++ IS T YLVY GC+ ATLSSAIASLVGAPRVLQA+ +DK+YP +  F+ GHG NN+PFRGYIL F IA  C+M+G L+ + SL SNFF+AAYAL+N SVFH+SM KSPGWRP FR+YN+W+SL G  +CV +MFL+D   A  T A+V ILY  + Y KPEA+WGSS EA  F+N L N Y L+   DH+K YRPK+++ +GNP HR PL+DFA+LLTKK+SLL+CA+I  +    +  S+K     WL  H +++F+ +        G  + + L+GLGK  PNM++MGF ++   +    + Y+  L  A +  +++ ILRLP G D+S ++  E     EV  +                                  + +   K  +VF G++G  +P+ I+DD+  F+ KK+ G IDVWWLYDDGGLTLLLP IL+ RKQF +C+LRVF L +  +D DSET+++ +LL KFRI + +V++IPD+T +A   T+ +F +I SK    + DM  L  N ER+NR+LR +E L+E S NS+++V+TLP+PR    S  +Y++WL++MTK L  P L  RGNQSSVLTFYS
Sbjct:  342 LSGDLKDPSTSIPKG--------------------TLYAIIVTYISYAFFAVLVGFNFLPQASGNVIEYTN--NTGMYPEIFNCSEEANRIREEFNLSATSCKYGSAHDQKTMTLISATGYLVYLGCYGATLSSAIASLVGAPRVLQAVGRDKIYPKLEFFAVGHGANNDPFRGYILVFVIAFGCMMIGELNTIGSLASNFFLAAYALMNLSVFHSSMTKSPGWRPTFRFYNKWLSLFGAIVCVVLMFLLDWRLACGTTALVAILYGIMYYLKPEANWGSSAEALVFLNALNNTYALNEQQDHIKTYRPKVLLLTGNPIHRQPLLDFANLLTKKVSLLICANIVNEDEKDQITSMKESVELWLKDHNIRSFYSIIPNNKLSLGVQNSILLSGLGKLTPNMVIMGFNSN-NQNIHSIQEYFQTLVFAFENKMAVGILRLPNGNDYSSYIATEETTIEEVEAN---------------------------------KKGKKDKKVTAVFRGKDGKVLPKQIVDDITQFRTKKREGFIDVWWLYDDGGLTLLLPYILQTRKQFRDCKLRVFLLSNKAEDFDSETRSLANLLAKFRISFSEVLVIPDITKKAKPETKAEFEEIVSKYPKDSVDMSELNANAERTNRHLRTAELLREKSANSELIVLTLPLPRIGS-SPSLYLAWLDVMTKGLP-PVLLTRGNQSSVLTFYS 990          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1067092449|ref|XP_018008040.1| (PREDICTED: bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Hyalella azteca])

HSP 1 Score: 602.823 bits (1553), Expect = 0.000e+0
Identity = 309/721 (42.86%), Postives = 443/721 (61.44%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVF---SFNNC-------------TFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADIREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDW-LDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSK-----------AGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFY 693
            +SGDLKDP  AIPKG                    TL A+ +T+ SY+ Y  ++ +  + +ASG+   +  F   N T+ +    +F  C             T   CE+G  N  Q +  ++    L+Y GCFAATLSSAIASLVGAPRVLQALAKD+LYP I +FS G G NN+P  GY L F +A  C+M+GNL+ VS+LLSNFF+A+Y+LINFS FHAS +K   +RP F+YYN WVSL G  LC+  MFL+D +TA IT  V   LY ++ YRKP+ +WGSST++  +++ L     L    DHVKNYRP+I+V SG PS R PL+DFA+L+TK LS+++C ++ +D+  K +       N+WL++ K KAF+ +      ++GA++   LAGLGK  PN++L+GFK+DW + D+   + Y+N LH A +M L + ILRL  G D+S  +                     +D+   +  G+  + + I      ++++R  S   + +TG  G  +P+ ILD + +FK+K+  G IDVWWLYDDGGLTLLLP IL  R  ++NC+LR+FAL +  D+LD E ++M +LL KFRIDY DV++IPD  +  S+A++ +F  +  K             IT + L   ++++NR++RL E L E S+ + +++MTLPMPR   +S+ +YM+WL+ M+K +  PFLFVRGNQ SVLTFY
Sbjct:  315 LSGDLKDPGSAIPKG--------------------TLLAIGITFCSYILYPFLLAANVVRDASGNTTAFKEFYYTNETVNILDSPTFTECSTTGYDYVTEDNETVLACEFGLHNSFQVMELVAAWGPLIYIGCFAATLSSAIASLVGAPRVLQALAKDRLYPGIFMFSKGFGANNDPVNGYCLVFVLAFLCIMIGNLNAVSTLLSNFFLASYSLINFSCFHASFIKIGRFRPIFQYYNLWVSLFGGVLCLVTMFLIDWITALITFLVTIGLYLFVSYRKPDVNWGSSTQSASYVSALRATLTLQTVVDHVKNYRPQILVMSGRPSSRPPLLDFANLITKNLSMMVCVEVSKDMDWKTRTVYINKCNDWLIEKKYKAFYNLVKADRLDEGAVNLFQLAGLGKLKPNVVLLGFKSDWSVCDKQELKAYFNTLHAAFNMQLGIAILRLSNGTDYSNIIA--------------------DDESPKISEGIVNEGADIETTPRAEAKKRKFS-FSAAYTGPGGAKLPKEILDGVTIFKRKQGEGTIDVWWLYDDGGLTLLLPYILTTRSNWSNCKLRIFALANRRDELDMEQRSMANLLSKFRIDYGDVIVIPDAMSGVSDASRREFQGLIEKFRTTAKCADDGVSITEEELANQKDKTNRHIRLKELLLENSSTASLIIMTLPMPRKGQVSASLYMAWLDYMSKGM-PPFLFVRGNQQSVLTFY 993          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|939255525|ref|XP_014247518.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Cimex lectularius])

HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0
Identity = 322/721 (44.66%), Postives = 442/721 (61.30%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCAD-IREDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDG--AMSCMNLAGLGKFAPNMILMGFKTDW-LDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQ---------HECIVEVEVTNDEEDDLCYDEDDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRR---SVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDIS----------SKAGITTDMLIE-NQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP+VAIP+G                    TL A+I T++SY+ YA M+G C L EASG+           + ++ +   +     C YG  ND Q +  +S    L+Y GCFAATLSSAIASLVGAPRVLQALAKDKLYPFIG FS G G NN+P RGYIL F IA  C+++GNL+ ++ LLSNFF+AAYALINFSVFH S+ KSPGWRPAF+YYN WVSL GT LC+AVMFL+   TA +T  VV  LY Y+ YRKPE +WGSST+AQ +   L +  +L+   +HVKNYRP+I+V SG P HR  L DFA+L+TK  SLL+C+  I+   +    +++   A  W  +HK+KAF+ +  +    +   A S M +AGLGK  PNMILMG+K DW +  +   + Y+ ++H ALD  L++ ILR+  G D+S  ++         +E   E E      +        D+    G      S + D+ +   +   S  +    +F G  G  +    L  L  F++K+   +IDVWWLYDDGGLTLL+P I+  R Q+++C+LR+F+L +  ++LD   +N+ +LL KFRIDY D++IIPD+T +A   TQ +F DI           SK G   D  ++   E++NR++RL E L EYS +    V+TLPMPR   +S+ +YM+WLE++T  +  P L +RGNQ+SVLT+YS
Sbjct:  350 LSGDLKDPSVAIPRG--------------------TLLAIITTFISYISYAFMMGGCVLREASGNETILENIEPGTSLIDRWDKISQCNTTCTYGLRNDNQAMELVSLWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGFFSKGFGANNDPIRGYILVFVIAFGCILIGNLNYIAPLLSNFFLAAYALINFSVFHVSISKSPGWRPAFKYYNAWVSLVGTLLCIAVMFLISWWTALVTFFVVVALYLYVSYRKPEVNWGSSTQAQAYYFALRSALELNKVAEHVKNYRPQILVLSGMPGHRPTLTDFANLITKGKSLLVCSHIIKGSYSRTVHEALSQKAYRWFEQHKIKAFYILGEDDGIGEHYLAKSFMTMAGLGKLKPNMILMGYKNDWQICPKQELDNYFTLIHAALDSFLAVGILRMADGLDYSNMIEDADSPLFPSYEKKEEDEEDKLHRNISAGQLSLDDACSAGSISPPGSPMLDQEEGIEEEENSSGKDPTEMFRGVRGETLSTHHLQALTYFRRKQSKALIDVWWLYDDGGLTLLIPHIISTRSQYSDCKLRIFSLANRANELDRAHRNLAALLSKFRIDYSDLIIIPDITKKAKPETQKEFEDIIKNFRKLDGSFSKNGYIADSELKLLAEKTNRHMRLRELLHEYSKDVTFYVVTLPMPRKGTVSAPMYMAWLELITAGM-PPALVLRGNQTSVLTYYS 1049          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1233209917|ref|XP_022204235.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Nilaparvata lugens])

HSP 1 Score: 600.897 bits (1548), Expect = 0.000e+0
Identity = 324/740 (43.78%), Postives = 447/740 (60.41%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDN-------ATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI-REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEK---NFEDGAMSCMNLAGLGKFAPNMILMGFKTDW----LDDEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHECIVEVEVTN--DEEDDLCYDED------DDEDLDTGMTLKISSILGDKLKKSRQRHRSK---------RRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDISSK--------------AGITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP+ AIP+G                    TL A++ T+VSY+ YA+M+G C L +ASG+       L+          T+      N +   C YG  ND Q +  +S    L+Y GCFAATLSSAIASLVGAPRVLQALAKDKLYPFI  FS G+G NN+P RGYIL FCIA  CV++  L+ ++ LLSNFF+AAYALINFSVFHAS+ K PGWRPAF+YYN WVSL GT LC+AVMFL+   TA +T  VV  LY Y+ YRKPE +WGSST+AQ +   L +  +L+   +HVKNYRP+++  SG+P  R  ++DFA+L+TK  SLL+C  I + + + +  D++   A  WL +H++KAF+ V  E+    FE  A + M L GLGK  PN +LMGFK +W    LD+ +    Y+N++H ALD +L++ IL L  G D+S +VQ + ++ +   +  D E+ L  ++       D E  D        +   D       +   K            +F G +G  +   +L D+  F++K+  G IDVWWL+DDGGLTLL+P IL+ R QFA+ +LR+F+L S    LD + KN+  LL KFRIDY D+ +I D+   A   T+  F ++  K                IT + L+   E++NR+LRL E L E+S +S +++MTLPMPR   +S+ +Y+SWLE ++  L  P L +RGNQ+SVLT+YS
Sbjct:  368 LSGDLKDPSKAIPRG--------------------TLLAILTTFVSYLSYALMMGGCVLRDASGNVTALREALDSGLAPPALYMTISQTCLGNNS-AACRYGLHNDNQAMELVSVWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIYFFSKGYGANNDPIRGYILVFCIAFGCVLIAQLNAIAPLLSNFFLAAYALINFSVFHASISKCPGWRPAFKYYNAWVSLIGTLLCIAVMFLISWWTALVTFFVVVTLYLYVSYRKPEVNWGSSTQAQSYNFALRSALELNRVQEHVKNYRPQVLALSGDPGSRPAMIDFANLITKGNSLLVCGHILKGNHSQRVHDALTQKAQRWLDQHRIKAFYAVTSERTGGTFETSAEALMQLTGLGKLKPNTVLMGFKHNWHTCHLDELV---NYFNLIHVALDSHLAVGILCLENGLDYSNYVQDKDVILISSLDKKDAEEKLRRNQSAGQLSLDGEQSDIAPAAAAVNTHPDPNGGDVTQQLDKDSESSVSESGGDLFVGPSGEPLAPNLLKDITRFRRKQDKGFIDVWWLFDDGGLTLLVPHILRTRSQFADSKLRIFSLASRKSQLDHDQKNLAQLLLKFRIDYSDLTVISDINKPARSETRLQFENLIEKFRLRGNDPAKADGSKIITDNELVLLHEKTNRHLRLRELLFEHSLDSTLIIMTLPMPRKGTVSAPLYLSWLETLSHGL-PPTLILRGNQTSVLTYYS 1082          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|646699947|gb|KDR10326.1| (Solute carrier family 12 member 2, partial [Zootermopsis nevadensis])

HSP 1 Score: 598.201 bits (1541), Expect = 0.000e+0
Identity = 325/729 (44.58%), Postives = 463/729 (63.51%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAE-YNAFLNDNATLEVFSFNNCTFRKCEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI-REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLDDEMGT-ERYYNILHQALDMNLSLTILRLPRGCDFSQHVQHE-----CIVEVEVTNDEEDDLCYDEDDDEDLDTGMTL-KISSILGDKLKK----SRQRHRSKRRS------VFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDF---------LDISSKAG-------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDL DP  AIPKG                    TL +++LT +SY+  A M G+    +ASG+  E YN            +F NCT+R+C++G  N  Q +  +S    L+YAGCFAATLSSA+ASLV AP+V QAL KD+LYP+I  F+ G+GKNNEP RGY+L F I++  +++G L+ ++ L+SNFF+AAYALINFS FHAS+ K  GWRP FR+YN W+SLAG  LCV VMFL+   TA IT+A V  LY  + YRKP+ +WGS+T+AQ + N L +V  L+   DHVKNYRP+I+V SG PS R PL+DFA+LLTK LSLL+C  I +  V+ + ++ +   A+ WL  HK+KAF+    E NFE+GA + +  +G+GK  PN++LMG+K DW + E      Y+ ++H+ALDM +++ ILRL  G D+S+ +  E      I  V    D +  +   ++  E L    +  ++S   G++  K    S    R ++R       ++ G  G  +P+ +L++L  F++K+K G IDVWWLYDDGGLTLLLP I+  R+ ++ C+LRVFAL +  D+L+ E ++M SLL KFRIDY D+++IPD+T +  E T + F         ++ S+  G       IT   L+   +++NR+LRL E + E S  + ++VMTLPMPR +++S+ +YM+WLE +TKD+  PFL VRGNQ+SVLTFYS
Sbjct:  294 ISGDLSDPQGAIPKG--------------------TLLSILLTTLSYLSMAAMSGAVMERDASGNVTELYNG-----------TFTNCTYRECKWGLHNSFQVMELVSAFGPLIYAGCFAATLSSALASLVSAPKVFQALCKDQLYPYITWFAKGYGKNNEPVRGYVLCFFISIGFILIGELNAIAPLISNFFLAAYALINFSTFHASLAKPVGWRPKFRFYNMWLSLAGALLCVFVMFLISWWTALITLAFVLALYLIVAYRKPDVNWGSTTQAQTYKNALLSVQQLNNVEDHVKNYRPQILVLSGMPSFRPPLIDFAYLLTKNLSLLICGHILKGPVSQRIRNILTYKAHNWLRSHKIKAFYTHVDEVNFEEGAKALIQASGVGKLRPNILLMGYKGDWRECEREELAMYFEVMHKALDMYMAVAILRLQEGLDYSKVIADEDDLVNGISSVAGVGD-KGAIRTRQNSQESLPRNQSFSQMSQDSGNQESKNHAASGMNSRKEKRKLEYKADLYRGPGGSTLPKDVLNNLIQFQKKQKKGTIDVWWLYDDGGLTLLLPYIIGTRRNWSACKLRVFALSNKKDELEFEQRSMASLLAKFRIDYSDLMVIPDITKKPLETTVSFFESLISEFKQMEGSTDGGTSDDPMAITEAELLAMNDKTNRHLRLRELVMENSLEANLIVMTLPMPRKNVVSATLYMAWLETLTKDM-PPFLLVRGNQTSVLTFYS 989          

BLAST of sodium-potassium-chloride cotransporter vs. nr
Match: gi|1062667687|ref|XP_017956901.1| (PREDICTED: bumetanide-sensitive sodium-(potassium)-chloride cotransporter isoform X3 [Drosophila navojoa])

HSP 1 Score: 592.038 bits (1525), Expect = 0.000e+0
Identity = 327/763 (42.86%), Postives = 462/763 (60.55%), Query Frame = 0

Query:    1 MSGDLKDPAVAIPKGKPFKDPFQSNTCSPPEIPSGTLSAVILTYVSYVGYAIMIGSCYLSEASGDAAEYNAFLNDNATLEVFSFNNCTFRK---CEYGTSNDQQTLSKISYTSYLVYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIGVFSSGHGKNNEPFRGYILSFCIALACVMVGNLDVVSSLLSNFFVAAYALINFSVFHASMVKSPGWRPAFRYYNEWVSLAGTFLCVAVMFLMDTMTAFITIAVVGILYFYIRYRKPEAHWGSSTEAQQFMNTLENVYDLSLTPDHVKNYRPKIMVFSGNPSHRVPLMDFAHLLTKKLSLLMCADI-REDVTIKEKDSIKADANEWLVKHKMKAFHYVAYEKNFEDGAMSCMNLAGLGKFAPNMILMGFKTDWLD-DEMGTERYYNILHQALDMNLSLTILRLPRGCDFSQHV--------QHECIVEVEVT---NDEEDDLCYDE---------------------------------DDDEDLDTGMTLKISSILGDKLKKSRQRHRSKRRSVFTGQNGVAVPQVILDDLNVFKQKKKLGVIDVWWLYDDGGLTLLLPVILKMRKQFANCQLRVFALGSTVDDLDSETKNMTSLLEKFRIDYEDVVIIPDVTTEASEATQTDFLDI-------------SSKAG-------ITTDMLIENQERSNRYLRLSESLKEYSTNSQMVVMTLPMPRPSMISSGIYMSWLEIMTKDLDTPFLFVRGNQSSVLTFYS 694
            +SGDLKDP  +IPKG                    T+ A+++T  +Y+   ++ G     +A+G   +    LN +     F+F NC+      C YG  N  Q +  +S    L+YAGCFAATLSSA+ASLV AP+V QAL KD+LYP I  F+ G+GKNNEP RGY+L+F IA+  ++VG+L+ ++ L+SNFF+AAY LINFS FHAS+ K  GWRP F+YYN W+SL G+ LCVAVMFL+   TA IT   V  LY  + YRKP+ +WGS+T+AQ + N L +V  L+   DHVKNYRP+I+V SG P+ R  L+DFA++LTK LSLL+C  + R   + K ++ +K  A+ W  KH++K F+ +   ++FE G+ + M   G+GK  PN+ILMG+KTDW   D     +Y+N++H+ALDM LS+ ILR+P+G D SQ +           C +EV  T   N+  +DL   E                                  +   L   +  K ++ L + L+KS+ +H     S++ G  GV +P+ +L DL  F +K+   VIDVWWLYDDGGLTLLLP I+  R+ + +C+LRV+AL +   +L+ E ++M SLL KFRIDY D+ +IPD+T +  E +   F ++             SSKA        I+ D L+   +++NRYLRL E L+E ST S +VVMTLPMPR +++S+ +YM+WLE +++D+  PFLFVRGNQ+SVLTFYS
Sbjct:  427 ISGDLKDPQKSIPKG--------------------TILAIVITTATYMLMVLICGGTVARDATGYVVDA---LNGS-----FAFLNCSSTSTGTCLYGLQNSFQVIELVSAFGPLIYAGCFAATLSSALASLVSAPKVFQALCKDELYPKIVWFAKGYGKNNEPVRGYVLTFLIAMIFILVGDLNSIAPLISNFFLAAYMLINFSTFHASLAKPVGWRPTFKYYNMWLSLLGSILCVAVMFLISWATALITFCAVLALYLIVAYRKPDVNWGSTTQAQTYKNALMSVQQLNNVEDHVKNYRPQILVLSGLPNTRPVLVDFAYMLTKNLSLLVCGHVLRGSGSQKYRNYLKERASNWFQKHRVKGFYALIDGEDFEAGSRALMQATGIGKLKPNIILMGYKTDWQTCDRKELVQYFNVMHKALDMYLSVAILRVPKGLDCSQLLGSPDSNWTTANCSLEVPRTLQPNESSNDLQASEARHALSGSVDSLSRNVSQASSTSDLSFIAGSQVKEVSGLPDPLDAKTTNELPNTLRKSKAKHDDP-VSLYRGPGGVELPKDVLADLTQFTRKRSHAVIDVWWLYDDGGLTLLLPYIISTRRTWQSCKLRVYALANKNSELEFEQRSMASLLSKFRIDYSDLTLIPDITKKPQETSTQFFNELIKDFVVGDKENGHSSKATLNEDEALISDDDLLAVVDKTNRYLRLREYLREQSTKSDLVVMTLPMPRKNIVSAPLYMAWLESLSRDM-PPFLFVRGNQTSVLTFYS 1159