BLAST of alanine--trna cytoplasmic vs. L. salmonis genes
Match: EMLSAG00000005203 (supercontig:LSalAtl2s:LSalAtl2s274:26570:29820:-1 gene:EMLSAG00000005203 transcript:EMLSAT00000005203 description:"maker-LSalAtl2s274-augustus-gene-0.21")

HSP 1 Score: 1293.87 bits (3347), Expect = 0.000e+0
Identity = 626/930 (67.31%), Postives = 761/930 (81.83%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGV-RAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVE-NEECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSV-EGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQC 979
            M    +A E+R+ FLDFF + K H Y HSSSTIPLDDPTLLF N+GM QFK +FVG+IDPN+D AKL RA NSQKC+RAGGKHNDL+DVGKD YHHTFFEMLGNWSFGDYFKKEIC W+WEFLT+  ++P+DRLYV+YFGG+    L+PDL+CKQ WLDLGVPE ++LPG+M+DNFWEMGDTGPCGPCSEIHFDR+GGRNAAHLVN DDP VLEIWNLVF+Q+NRE DKSL++LP+KHIDCG+G ERLVS +QN  SNYDTD+F P+FEAI + +G  + Y GK GDED D++DMAYRV+ADHIRTLTI ++DGGRPDNVGRGYVLRRILRR IRFATEKL+AKPGFF  LVDVVV +LG  FPE+ KDP+ IKD+I+EEE QFLKTL+RGRKLLDRTI K+   +K+LPGD+AWRLYDTYGFP+DLTQLM EER + VD+E YE AK+ A L SQG     ED++ALDVH+I ELQDK +  T+D  KY Y+ +S +  + Y F   +  ++ LR +K FVD +    ECG++LD T FYAE GGQI+DEGF+VK +D+S+E KV NVQVRAG+VLH G++ EG LK+G+ + LQ+D  RR+NVM NHTGTHILNFALR  LE ++DQ+GSLVA D+LRFDFT  KA+T +QVK +E   N+II KNEE++AKEASLA+AK IQGLRAVFDETYPDPVRVVSVGI VEK+EADPT  AGS TSVEFCGGTHL+R+GH+  F+++SEEAIAKG+RRIIALTGPEA KA QKA LLQ ++++V   ++  K  ++ KEMVKLIT+L+EDI+++TIS WKKDEMRN+LKA+KKTIDD+ +A+KAA++++ VE  K ++ +N N  FLV ELNA AQNK LDGALK +K+ +    A+F S D DTGK+LCMAQV KE   + LKANEWCQQ 
Sbjct:    1 MDSSLKADEIRKLFLDFFSKKKDHTYYHSSSTIPLDDPTLLFINAGMNQFKSVFVGTIDPNNDKAKLVRAVNSQKCVRAGGKHNDLEDVGKDSYHHTFFEMLGNWSFGDYFKKEICEWSWEFLTKELKMPEDRLYVSYFGGNEKTGLDPDLDCKQIWLDLGVPEERLLPGNMKDNFWEMGDTGPCGPCSEIHFDRVGGRNAAHLVNQDDPMVLEIWNLVFIQFNREADKSLKLLPKKHIDCGLGLERLVSAIQNKSSNYDTDIFTPIFEAIHERSGASKPYQGKFGDEDPDQIDMAYRVVADHIRTLTIVIADGGRPDNVGRGYVLRRILRRGIRFATEKLNAKPGFFGSLVDVVVSVLGHIFPELTKDPELIKDVINEEEIQFLKTLNRGRKLLDRTIGKMD--SKILPGDVAWRLYDTYGFPIDLTQLMVEERNMTVDLEGYEKAKEKAVLMSQGKGAGTEDRLALDVHSIQELQDKSISTTDDKFKYHYEEKSSDFDSDYEFSGIEATIMALRYEKKFVDSIACGTECGIILDHTSFYAEQGGQIYDEGFIVKVNDDSVEFKVKNVQVRAGYVLHVGTLGEGELKIGEKVFLQIDTIRRQNVMKNHTGTHILNFALRQVLEGDSDQKGSLVASDKLRFDFTCKKAMTTDQVKAVEMCVNEIIRKNEEIFAKEASLAVAKTIQGLRAVFDETYPDPVRVVSVGIRVEKMEADPTGAAGSVTSVEFCGGTHLKRAGHLQVFVISSEEAIAKGIRRIIALTGPEAKKATQKAVLLQNKVDKVSLVVQEAK--LSSKEMVKLITNLSEDISSSTISQWKKDEMRNSLKALKKTIDDKSKAKKAAVLSQIVEEAKIMVGNNENAPFLVKELNAFAQNKALDGALKVIKS-SNIPAALFISGDEDTGKVLCMAQVNKELTSK-LKANEWCQQV 924          

BLAST of alanine--trna cytoplasmic vs. L. salmonis genes
Match: EMLSAG00000005940 (supercontig:LSalAtl2s:LSalAtl2s320:546129:548925:-1 gene:EMLSAG00000005940 transcript:EMLSAT00000005940 description:"maker-LSalAtl2s320-augustus-gene-4.11")

HSP 1 Score: 308.145 bits (788), Expect = 5.877e-90
Identity = 237/759 (31.23%), Postives = 363/759 (47.83%), Query Frame = 0

Query:  153 MLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKL-DMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKP--GFFSGLVDVVVELLGPTFPEVKKDPQTIKD-----IIDEEETQFLKTLSRGRK-LLDRTIAKLPEGT----------------------------------KVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGL------RKDKTFVDQVENEECGVLL-DKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVE-GSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTA---NKALTIEQVKKIETVSNQIIGKNEE-VYAKEASL--AIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDL 854
            MLG+WSF DYFKK+   +AWEFLT   E+PK+ L VTYF        + DLE +++WL  G+   KI+P    DNFWEMG TGPCGPC+EIH+                    EIWNLVFM+Y R  +  L  L +KH+D GMG ERL SIL    SNYDTD+F P+F+ I K +G   Y+G       DKL D+ YR++ADH R +   LSDG  PD     Y LR I+RR ++ + E  ++K    F   L+  V E LG    E  + P  +KD     ++ + E   LK L +  K LL++    +P  T                                  K++ G+ A++LYD+ GF  +  +L +     K D   YE    +A++  Q    T  ++  +++ +   L  K    T+D  KY++     +   KY F   +  ++ L      + D   V ++   + G+L+ +K+ FY+EAGGQ    G +  +  ES+   V +V    G V H G+V  G+    D++ L++D  +R   M NHTG+H+LN A+ + L     QR   V  D   F F A      L+  ++  IE  +NQ+I   +  V AK+  L   I  +   +  +  E YPD V ++ +             P  S    E C GTH+  +  +    +   ++++ G++    LTG +A+ A +        +E V++ +E      +++E   L+ D+
Sbjct:    1 MLGSWSFNDYFKKDAIQFAWEFLTGPLEIPKEFLNVTYF--------KDDLETREYWLSQGLSPDKIIPKGETDNFWEMGSTGPCGPCTEIHYKDT-----------------EIWNLVFMEYVRRPNGELASLDKKHVDTGMGLERLTSILNGYTSNYDTDLFIPIFDKISKLSGADPYSGSF-----DKLQDINYRILADHSRMIATCLSDGLLPD---VSYRLRNIIRRCMKISAESFNSKNQVDFLKELIYCVSESLGDHHREYLESP-FLKDEAKLSLLLKFEHDVLKELEKTHKSLLNKLKDAVPFKTLFEKYESELDPSMIGRFYDAFKMYSCNALNNAEPKIISGEFAFKLYDSIGFKSEDIELFALLLDAKYD--PYEFHLYSAKVKEQSKVTTSVNRTNMEILSKITLAPK----TDDSFKYKWV----QSGFKYQFYIIESNILQLIQISNDKSDSQCVGKLNRGDTGILICEKSPFYSEAGGQAXXTGSITGSLGESV-FHVHDVFSLKGVVYHVGTVHSGTFMRQDLVKLKIDPFKRVTHMQNHTGSHLLNAAMNTVLPFTT-QRSLRV--DXTGFKFXACIFKLNLSSSEINDIEEKANQMINNADPIVQAKDKYLDSFIKNSKSKVLTLPGEKYPDKVSMICL-------------PQFS----EPCCGTHVNCTKDVEALTITHIKSVSSGIKSFRCLTGQKAIDARENGLAFLDNVEFVRQRLEELGNYPSKEEQNNLLKDI 694          

BLAST of alanine--trna cytoplasmic vs. L. salmonis genes
Match: EMLSAG00000002760 (supercontig:LSalAtl2s:LSalAtl2s15:136097:142061:1 gene:EMLSAG00000002760 transcript:EMLSAT00000002760 description:"maker-LSalAtl2s15-augustus-gene-1.12")

HSP 1 Score: 55.8398 bits (133), Expect = 4.284e-8
Identity = 46/150 (30.67%), Postives = 68/150 (45.33%), Query Frame = 0

Query: 1000 VEKRPCAQVPLRLEIAQDCILTHPIAIATYLAGESPLGRGYSQSDRSSILQWLNFARDSVHN---------VMSLVANGQSQPLTLAAALDLMGSLDQILERRTFLVGQRISLADAFLAVECLPL---CQDPNLRINRKNFPHLLRWLNT 1137
            ++K P  +VP   E ++   LT   AIA  +A E    RG +  +R+ I QWL+F   ++           + ++  N +S           +  L+      T+LVG+RISLAD  L    L L     DP     RK FP++ RW NT
Sbjct:   49 LKKFPSGKVP-AFESSEGVTLTESNAIAYLIASEEL--RGKTPVERAQIQQWLDFTDSNILPPALTWVFPCLGAMAYNKKSMDRAKEDVGKALKVLNDHFLLHTYLVGERISLADISLTCSILSLYVHVMDPEF---RKPFPNVERWFNT 192          

BLAST of alanine--trna cytoplasmic vs. SwissProt
Match: gi|135089|sp|P21894.1|SYAC_BOMMO (RecName: Full=Alanine--tRNA ligase, cytoplasmic; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 1244.57 bits (3219), Expect = 0.000e+0
Identity = 589/930 (63.33%), Postives = 718/930 (77.20%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVEN-EECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQCID 981
            M      +E+R+ F+DFF++ K H Y HSSSTIPLDDPTLLFAN+GM QFKPIF+GS+DPNSDMA+  R  N+QKCIRAGGKHNDLDDVGKDVYHHTFFEM+GNWSFGDYFKKEIC WAWE LT+V +L ++RLYVTYF GD ++ LEPDLEC+  WL+LGVPEA ILPGSM+DNFWEMG+TGPCGPCSE+H+DRIG R AAHLVNMDDPDVLEIWNLVF+Q+NRE D SL++LP KHIDCG+G ERLVS++QN R+NYDTD F P+F+AI+  TGVR Y+GK+G +D D +DMAYRV+ADH RTLTIALSDGG PDN GRGYVLRRILRRA+R+A+EKL+AKPGFF  LV  VVELLG  FPE+KKDP +I  +I+EEE QFLKTL RGR LL RTI KL   +K LPGD+AWRLYDTYGFP+DLTQLM EE+GL VD+E YE +++ +QL SQG +   ED IALDVHAI+ LQD G+  T+D PKY Y   S +  A Y F  C  K++ LRK+K FV ++ + +ECGV+LD+T FYAE GGQIFDEG+MVK DDE++E  V NVQV+ G+VLH G VEG LKVGD L+L +D  RR+ VMNNHTGTH+LN  LR  L  ++DQRGSLV PDRLRFDFT    +TI+Q+K  E    +II KN+ VYA   SL+ AK I GLRA+FDE YPDPVRVVSVG+PVE L  +P  P G  TSVEFCGG+HL R+ HIG +++ SEE IAKG+RRI+A+TGPEA+KAI K  +L+ ++  V   I+    +++ KE+ K I DLT +I+ A ISYWKKDE+RN LK +KK +DD++RA KA ++T+  E  K+L        ++V EL A    K LDGALKQVK   P++ AMFFS D D  KI C+A V K  + +GL A+EW Q  +D
Sbjct:    1 MDTSMTGNEIRKTFIDFFIK-KGHKYVHSSSTIPLDDPTLLFANAGMNQFKPIFLGSVDPNSDMAQYIRVVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMMGNWSFGDYFKKEICAWAWELLTDVFKLSRERLYVTYFEGDPSSGLEPDLECRNIWLNLGVPEAHILPGSMKDNFWEMGETGPCGPCSELHYDRIGDREAAHLVNMDDPDVLEIWNLVFIQFNRETDGSLKLLPTKHIDCGLGLERLVSVIQNKRANYDTDFFMPIFKAIENATGVRPYSGKVGVDDVDGIDMAYRVLADHARTLTIALSDGGCPDNTGRGYVLRRILRRAVRYASEKLNAKPGFFGSLVYTVVELLGDVFPEIKKDPDSIVHVINEEEVQFLKTLLRGRNLLYRTIEKL-NNSKTLPGDVAWRLYDTYGFPIDLTQLMCEEKGLNVDMEGYEKSRKESQLVSQGKAAGQEDLIALDVHAISHLQDTGIPATDDSPKYNYLPSSTDKDALYTFAPCTAKIVALRKNKEFVSEISSGQECGVILDRTSFYAEQGGQIFDEGYMVKIDDETVEFTVKNVQVKGGYVLHAGKVEGILKVGDTLSLHIDTERRRLVMNNHTGTHVLNNVLRKVLGNDSDQRGSLVMPDRLRFDFTNKGPMTIKQIKDTENEIKEIIAKNKTVYANYTSLSEAKKINGLRAMFDEHYPDPVRVVSVGVPVEDLIKNPDAPTGFETSVEFCGGSHLHRTSHIGEYVIVSEEGIAKGIRRIVAVTGPEAIKAINKLSVLENEVNNVANFIKEQNESISHKEVSKKIVDLTNEISQAQISYWKKDELRNMLKNLKKQLDDKERAEKAIIITQVTEKAKELCLERKESKYIVSELKAFGNTKALDGALKQVKQFCPNSAAMFFSVDKDADKIYCLAAVPKSDVEKGLLASEWVQSVVD 928          

BLAST of alanine--trna cytoplasmic vs. SwissProt
Match: gi|75027177|sp|Q9VLM8.1|SYAC_DROME (RecName: Full=Alanine--tRNA ligase, cytoplasmic; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 1243.41 bits (3216), Expect = 0.000e+0
Identity = 583/922 (63.23%), Postives = 720/922 (78.09%), Query Frame = 0

Query:   59 AHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQV-ENEECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQC 979
            A EVR A+LDFF + K H Y HSSSTIPLDDPTLLFAN+GM QFKPIF+G+ DPNS+M+K  R  N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEIC+WAWEFLT+   LPKDRLYVTYFGGDA + LEPDLECKQ WLDLG+    ILPGSM+DNFWEMG+TGPCGPCSE+HFDRIGGR+   LVNMDDPDVLEIWNLVF+QYNRE D SL+ LP+KHIDCGMGFERLVS++QN RSNYDTD+F PLF+AIQ GTG   Y G++G +D D +DMAYRV+ADH RT+TIAL+DGG PDN GRGYVLRRILRRA+R+ATEKL+AKPGFF+ LV+ VV+LLG  FPEVKKDPQ I DII+EEE QFLKTL+RGR LL+RTI KL   T + PGD+AWRLYDTYGFPVDLTQLM+EE+ LK+D++ YE AK  + + SQG   +  ++I LDVHAI++LQ++GV PTND  KY+Y+A SDE  + Y +G C  K++ LR +  FV+++   ++ G++LDKT FYAE+GGQI+D+G +VK +DE+ E  V  V  R G++LH G VEG+LKVGD L L +D  RR   M NH+ TH LN  L   L  + +Q+GSLV P++LRFDF +  A+TIEQV K E ++ +++ KN  +YAKE+ LA+AK I+GLR+VFDE YPDPVRV+S G+PV++LE +P + AG  TSVEFCGGTHLRRSGHI  F+++SEEAIAKG+RRI+ALTGPEA+KA++K++  ++++  +K TI++ K     K  VK I +LTE I+ ATI Y KKDEMRN LK +KKT+DD++RA +AA+    VE  K L  +NPN   LV +L A    K LD ALKQV++  P   AMF S D D+ KI C++ V K  + +GLKANEW Q  
Sbjct:    6 AKEVRNAYLDFFKEQK-HIYVHSSSTIPLDDPTLLFANAGMNQFKPIFLGTADPNSEMSKWVRVANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICSWAWEFLTQRLALPKDRLYVTYFGGDAASGLEPDLECKQMWLDLGLKPEHILPGSMKDNFWEMGETGPCGPCSELHFDRIGGRSVPELVNMDDPDVLEIWNLVFIQYNRESDGSLKQLPKKHIDCGMGFERLVSVIQNKRSNYDTDLFVPLFDAIQAGTGAPPYQGRVGADDVDGIDMAYRVLADHARTITIALADGGTPDNTGRGYVLRRILRRAVRYATEKLNAKPGFFATLVNTVVDLLGDAFPEVKKDPQHIIDIINEEELQFLKTLTRGRNLLNRTIEKLGNQTTI-PGDVAWRLYDTYGFPVDLTQLMAEEKSLKIDMDGYEAAKHNSYVLSQGKGASKIEEINLDVHAISQLQEQGVPPTNDTFKYKYEAVSDERDSAYNYGVCNSKIVALRFENQFVNEITSGQKAGIVLDKTNFYAESGGQIYDQGALVKVNDEANEFLVDRVYNRGGYILHIGVVEGTLKVGDELELHIDVERRWLTMKNHSATHALNHCLLQVLGKDTEQKGSLVVPEKLRFDFNSKAAMTIEQVSKTEQLTKEMVYKNVPIYAKESKLALAKKIRGLRSVFDEVYPDPVRVISFGVPVDELEQNPDSEAGEQTSVEFCGGTHLRRSGHIMDFVISSEEAIAKGIRRIVALTGPEALKALKKSEAFEQEIVRLKATIDNDKSGKDSKSHVKEIVELTEQISHATIPYVKKDEMRNLLKGLKKTLDDKERALRAAVSVTVVERAKTLCEANPNATVLVEQLEAFNNTKALDAALKQVRSQLPDAAAMFLSVDADSKKIFCLSSVPKSAVEKGLKANEWVQHV 925          

BLAST of alanine--trna cytoplasmic vs. SwissProt
Match: gi|47117019|sp|Q8BGQ7.1|SYAC_MOUSE (RecName: Full=Alanine--tRNA ligase, cytoplasmic; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 1194.49 bits (3089), Expect = 0.000e+0
Identity = 574/930 (61.72%), Postives = 711/930 (76.45%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQ-VENEECGVLLDKTCFYAEAGGQIFDEGFMVKADDES---IELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQ 978
            M     A E+R+ F++FF + + H Y HSS+TIPLDDPTLLFAN+GM QFKPIF+ +IDP+  MAKLSRA N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLG+WSFGDYFK+  C  A E LT+   +P +RLYVTYFGGD  A LEPDLEC+Q W +LG+ EA+ILPG+M+DNFWEMGDTGPCGPCSEIH+DRIGGR+AAHLVN DDP+VLEIWNLVF+QYNRE D  L+ LP+K ID GMG ERLVS+LQN  SNYDTD+F P FEAIQKGTG R Y GK+G EDAD +DMAYRV+ADH RT+T+AL+DGGRPDN GRGYVLRRILRRA+R++ EKL+A  GFF+ LVDVVV+ LG  FPE+KKDP+ +KDII+EEE QFLKTLSRGR++LDR I  L +  K +PGD AW LYDTYGFPVDLT L++EE+GL VD+  +E  ++ AQL SQG     ED I LD++AI EL+ KG++ T+D PKY Y+++S   S  YVF      V+ LR++K FVD+ V  +ECGV+LDKTCFYAE GGQI+DEG++VK DD S    E  V N QVR G+VLH G++ G+LKVGD + L +DE RR+ VM+NHT THILNFALRS L  EADQ+GSLVAPDRLRFDFTA  A++ +Q+KK E + N +I   + VY ++  LA AKAIQGLRAVFDETYPDPVRVVS+G+PV +L  DP  PAGS TSVEFCGGTHLR S H G F++ +EEAIAKG+RRI+A+TG EA KA++K++ L+K +  ++  +++   T   K++ + I DL E +  A I  W+KDE R  LK++KK +DD DRA KA +    +E TKQL+ SNPN   ++LE+ + A  K L+ ALK  K  +P T AM F+ D + GKI C+ QV +    +GLKA+EW QQ
Sbjct:    1 MDATLTAREIRERFINFFRRNE-HTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEARILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFMPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPEMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGD-CKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMNGFEEERRLAQLKSQGKGAGDEDLIMLDIYAIEELRAKGLEATDDSPKYNYQSDS---SGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLKVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVL-GEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIVNGMIEAAKPVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPCGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEAKVKAQ--TAPNKDVQREIADLGEALATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQ 922          

BLAST of alanine--trna cytoplasmic vs. SwissProt
Match: gi|81871231|sp|Q8CFX8.1|SYAC_MESAU (RecName: Full=Alanine--tRNA ligase, cytoplasmic; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 1190.25 bits (3078), Expect = 0.000e+0
Identity = 572/930 (61.51%), Postives = 712/930 (76.56%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQ-VENEECGVLLDKTCFYAEAGGQIFDEGFMVKADDES---IELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQ 978
            M  P  A E+R+ F++FF + + H Y HSS+TIPLDDPTLLFAN+GM QFKPIF+ +IDP+  +AKLSRA N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLG+WSFGDYFK+  C  A E LT+   +P +RLYVTYFGGD  A LEPDLECKQ W +LG+ +AKILPG+M+DNFWEMGDTGPCGPCSEIH+DRIGGR+AAHLVN DDP+VLEIWNLVF+QYNRE D  L+ LP+K ID GMG ERLVS+LQN  SNYDTD+F P FEAIQKGTG R Y GK+G EDAD +DMAYRV+ADH RT+T+AL+DGGRPDN GRGYVLRRILRRA+R++ EKL+A  GFF+ LVDVVV+ LG  FPE+KKDP  +KDII+EEE QFLKTLSRGR++LDR I  L +  K +PGD AW LYDTYGFPVDLT L++EE+GL VD++ +E  ++ AQL SQG     ED I LD++AI EL+ KG++ T+D PKY Y ++S   S  YVF      V+ LR++K FV++ V  +ECGV+LDKTCFYAE GGQI+DEG++VK DD S    E  V N QVR G+VLH G++ G+LKVGD + L +DE RR+ VM+NHT THILNFALRS L  EADQ+GSLVAPDRLRFDFTA  A++ +Q+K+ E + N +I   + VY ++  LA AKAIQGLRAVFDETYPDPVRVVS+G+PV +L  DP+ PAGS TSVEFCGGTHL+ S H G F++ +EEAIAKG+RRI+A+TG EA KA++KA+ L++ +  ++  +++   T   K++ + I DL E +  A I  W+KDE R  LK++KK +DD DRA KA +    +E TKQL+ SNPN   ++LE+ + A  K L+ ALK  K  +P T AM F+ D + GKI C+ QV +    +GLKA+EW QQ
Sbjct:    1 MDSPLTAREIRERFINFFRRNE-HTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPLAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFAIPVERLYVTYFGGDEAAGLEPDLECKQIWQNLGLDDAKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRETDGLLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGD-CKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRAKGLEATDDSPKYNYHSDS---SGAYVFECTVATVLALRREKMFVEEVVTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLKVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVL-GEADQKGSLVAPDRLRFDFTAKGAMSTQQIKEAEEIVNAMIEAAKPVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLQNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESLRRSLSIMEAKVKAQ--TAPNKDVQREIADLGEALATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQ 922          

BLAST of alanine--trna cytoplasmic vs. SwissProt
Match: gi|115502460|sp|P49588.2|SYAC_HUMAN (RecName: Full=Alanine--tRNA ligase, cytoplasmic; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS; AltName: Full=Renal carcinoma antigen NY-REN-42)

HSP 1 Score: 1189.48 bits (3076), Expect = 0.000e+0
Identity = 573/931 (61.55%), Postives = 711/931 (76.37%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVE-NEECGVLLDKTCFYAEAGGQIFDEGFMVKADDES---IELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQC 979
            M     A E+RQ F+DFF + + H Y HSS+TIPLDDPTLLFAN+GM QFKPIF+ +IDP+  MAKLSRA N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLG+WSFGDYFK+  C  A E LT+   +P +RLYVTYFGGD  A LE DLECKQ W +LG+ + KILPG+M+DNFWEMGDTGPCGPCSEIH+DRIGGR+AAHLVN DDP+VLEIWNLVF+QYNRE D  L+ LP+K ID GMG ERLVS+LQN  SNYDTD+F P FEAIQKGTG R Y GK+G EDAD +DMAYRV+ADH RT+T+AL+DGGRPDN GRGYVLRRILRRA+R+A EKL+A  GFF+ LVDVVV+ LG  FPE+KKDP  +KDII+EEE QFLKTLSRGR++LDR I  L + +K +PGD AW LYDTYGFPVDLT L++EE+GL VD++ +E  ++ AQL SQG     ED I LD++AI EL+ +G++ T+D PKY Y  +S   S  YVF +    V+ LR++K FV++V   +ECGV+LDKTCFYAE GGQI+DEG++VK DD S    E  V N QVR G+VLH G++ G LKVGD + L +DE RR+ +M+NHT THILNFALRS L  EADQ+GSLVAPDRLRFDFTA  A++ +Q+KK E ++N++I   + VY ++  LA AKAIQGLRAVFDETYPDPVRVVS+G+PV +L  DP+ PAGS TSVEFCGGTHLR S H G F++ +EEAIAKG+RRI+A+TG EA KA++KA+ L+K +  ++  +++   T   K++ + I DL E +  A I  W+KDE+R  LK++KK +DD DRA KA +    +E TKQ + SNPN   ++LE+ + A  K L+ ALK  K  +P T AM F+ D + GKI C+ QV +    +GLKA+EW QQ 
Sbjct:    1 MDSTLTASEIRQRFIDFFKRNE-HTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGGDEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGD-SKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRARGLEVTDDSPKYNYHLDS---SGSYVFENTVATVMALRREKMFVEEVSTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVL-GEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKAVYTQDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAESLKKCLSVMEAKVKAQ--TAPNKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKRVLEKTKQFIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQV 923          

BLAST of alanine--trna cytoplasmic vs. SwissProt
Match: gi|261260086|sp|P50475.3|SYAC_RAT (RecName: Full=Alanine--tRNA ligase, cytoplasmic; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 1187.94 bits (3072), Expect = 0.000e+0
Identity = 576/932 (61.80%), Postives = 707/932 (75.86%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQ-VENEECGVLLDKTCFYAEAGGQIFDEGFMVKADDES---IELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQME--EVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQ 978
            M     A E+R+ F+++F + + H Y HSS+TIPLDDPTLLFAN+GM QFKPIF+ ++DP+  MAKLSRA N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLG+WSFGDYFK+  C  A E LT+   +P +RLYVTYFGGD  A LEPDLEC+Q W +LG+ EAKILPG+M+DNFWEMGDTGPCGPCSEIH+DRIGGR+AAHLVN DDP+VLEIWNLVF+QYNRE D  L+ LP+K ID GMG ERLVS+LQN  SNYDTD+F P FEAIQKGTG R Y GK+G ED D +DMAYRV+ADH RT+T+AL+DGGRPDN GRGYVLRRILRRA+R++ EKL+A  GFF+ LVDVVV+ LG  FPE+KKDP  +KDII+EEE QFLKTLSRGR++LDR I  L +  + +PGD AW LYDTYGFPVDLT L++EE+GL VD++ +E  ++ AQL SQG     ED I LD++AI EL+ KG++ T+D PKY Y ++S   S  YVF      V+ LR++K FVD+ V  +ECGV+LDKTCFYAE GGQIFDEG++VK DD S    E  V N QVR G+VLH G++ G+L+VGD + L +DE RR+ VM+NHT THILNFALRS L  +ADQ+GSLVAPDRLRFDFTA  A++ EQ+KK E + N +I   + VY  +  LA AKAIQGLRAVFDETYPDPVRVVS+G+PV +L  DP+ PAGS TSVEFCGGTHLR S H G F++ +EEAIAKG+RRI+A+TG EA KA++K++ L+K +   EVK   +S      QKE    I DL E +  A I  W+KDE R  LK++KK +DD DRA KA +    +E TKQL+ SNPN   ++LE+ + A  K L+ ALK  K  +P T AM F+ D + GKI C+ QV +    +GLKA+EW QQ
Sbjct:    1 MDSTLTAREIRERFINYFKRNE-HTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTVDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPVERLYVTYFGGDEAAGLEPDLECRQIWQNLGLDEAKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNRESDGVLKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDTDGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYSHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGD-CQTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRAKGLEATDDSPKYNYHSDS---SGSYVFECTVATVLALRREKMFVDEVVTGQECGVVLDKTCFYAEQGGQIFDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGNLRVGDQVRLFIDEPRRRPVMSNHTATHILNFALRSVL-GDADQKGSLVAPDRLRFDFTAKGAMSTEQIKKTEEIVNGMIEAAKPVYTLDCPLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKSETLKKSLSAMEVKVKAQSAPNKDVQKE----IADLGEVLATAVIPQWQKDEQRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKTHSPQTSAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQ 922          

BLAST of alanine--trna cytoplasmic vs. SwissProt
Match: gi|75042108|sp|Q5RC02.1|SYAC_PONAB (RecName: Full=Alanine--tRNA ligase, cytoplasmic; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS)

HSP 1 Score: 1187.17 bits (3070), Expect = 0.000e+0
Identity = 573/931 (61.55%), Postives = 711/931 (76.37%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVE-NEECGVLLDKTCFYAEAGGQIFDEGFMVKADDES---IELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQC 979
            M     A ++RQ F+DFF + + H Y HSS+TIPLDDPTLLFAN+GM QFKPIF+ +IDP+  MAK SRA N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLG+WSFGDYFK+  C  A E LT+   +P +RLYVTYFGGD  A LEPDLECKQ W +LG+ + KILPG+M+DNFWEMGDTGPCGPCSEIH+DRIGGR+AAHLVN DDP+VLEIWNLVF+QYNRE D  L+ LP+K ID GMG ERLVS+LQN  SNYDTD+F P FEAIQKGTG R Y GK+G EDAD +DMAYRV+ADH RT+T+AL+DGGRPDN GRGYVLRRILRRA+R+A EKL+A  GFF+ LVDVVV+ LG  FPE+KKDP  +KDII+EEE QFLKTLSRGR++LDR I  L + +K +PGD AW LYDTYGFPVDLT L++EE+GL VD++ +E  ++ AQL SQG     ED I LD++AI EL+ + ++ T+D PKY Y  +S   S  YVF +    V+ LR++K FV++V   +ECGV+LDKTCFYAE GGQI+DEG++VK DD S    E  V N QVR G+VLH G++ G LKVGD + L +DE RR+ +M+NHT THILNFALRS L  EADQ+GSLVAPDRLRFDFTA  A++ +Q+KK E ++N++I   + VY ++ SLA AKAIQGLRAVFDETYPDPVRVVS+G+PV +L  DP+ PAGS TSVEFCGGTHLR S H G F++ +EEAIAKG+RRI+A+TG EA KA++KA  L+K +  ++  +++   T   K++ + I DL E +  A I  W+KDE+R  LK++KK +DD DRA KA +    +E TKQL+ SNPN   ++LE+ + A  K L+ ALK  K  +P T AM F+ D + GKI C+ QV +    +GLKA+EW QQ 
Sbjct:    1 MDSTLTASKIRQRFIDFFKRNE-HTYIHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKPSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGGDEAAGLEPDLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLVDVVVQSLGDAFPELKKDPDMVKDIINEEEVQFLKTLSRGRRILDRKIQSLGD-SKTIPGDTAWLLYDTYGFPVDLTGLIAEEKGLVVDMDGFEEERKLAQLKSQGKGAGGEDLIMLDIYAIEELRARDLEVTDDSPKYNYHLDS---SGSYVFENTVATVMALRREKMFVEEVSTGQECGVVLDKTCFYAEQGGQIYDEGYLVKVDDSSEDKTEFTVKNAQVRGGYVLHIGTIYGDLKVGDQVWLFIDEPRRRPIMSNHTATHILNFALRSVL-GEADQKGSLVAPDRLRFDFTAKGAMSTQQIKKAEEIANEMIEAAKPVYTQDCSLAAAKAIQGLRAVFDETYPDPVRVVSIGVPVSELLDDPSGPAGSLTSVEFCGGTHLRNSSHAGAFVIVTEEAIAKGIRRIVAVTGAEAQKALRKAGSLKKPLSVMEAKVKAQ--TAPNKDVQREIADLGEALATAVIPQWQKDELRETLKSLKKVMDDLDRASKADVQKRVLEKTKQLIDSNPNQPLVILEMESGASAKALNEALKLFKMHSPQTAAMLFTVDNEAGKITCLCQVPQNAANRGLKASEWVQQV 923          

BLAST of alanine--trna cytoplasmic vs. SwissProt
Match: gi|74955985|sp|O01541.1|SYAC_CAEEL (RecName: Full=Alanine--tRNA ligase, cytoplasmic; AltName: Full=AlaRS A; AltName: Full=Alanyl-tRNA synthetase)

HSP 1 Score: 1130.55 bits (2923), Expect = 0.000e+0
Identity = 530/926 (57.24%), Postives = 677/926 (73.11%), Query Frame = 0

Query:   59 AHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQV-ENEECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKE----MVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQC 979
            A EVR  F++FF + K H Y HSSS IP DDPTLLFAN+GM QFKP+F+G  DPNSD+AKL RA N+QKCIRAGGKHNDLDDVGKDVYHHT+FEMLGNWSFGDYFKKEI TWAWE LT V  +P +RLYV+ FGGD    +  D E +  W  +GVP+ +IL   M+DNFWEMGD GPCGPCSEIH+DRIG R+A+HLVN DDP V+EIWNLVF+Q+NRE+   L+ LP KHIDCG+G ERL++++Q+  SNYDTD+FQP+FEAI KG+GVRAY G +GDED D +DMAYRV+ADH+RTLTIALSDGGRPDN GRGYVLRRILRR +R+A+EKL+A+PGFF+ LV VV+ +LG TFPE+ +DP T+ DII++EE QFLKTLSRGR L  R +  LPEGT   PGD++WRLYDTYGFP DLTQLM+EE+GL VD  A+E A++ A   S   +G   D + LDVHA+ ELQ KGV  T+D PKY Y    +   A Y F  C GK++ +R+D  FVDQ+   EE  +LLD+T FYAE GGQI+D G + K +DES E  V+N QVR G+++  GS EGS  VGD +N + DE R++ +M NHTGTH+LN+ALR  L A++DQ+GSLVAPDR+RFDFT    +T++QVKK E  + Q+I    +VYAK + L  AK ++GLRA+FDETYPDPVRVV+VG PVE+L  +P    G +T+VEFCGGTHL+   HIG  ++ASEEAIAKG+RRI+ALTGPEA +AI +A  L  ++EE  K  +     +  K+    + K I ++ ++ N A + YW+KD +R   KA++KT+D   +A++AA+  + +   K+L +       LV    A A +K +D ALK +K    +   M FS + D+GK+LC+A+V K  +  GLKANEW  + 
Sbjct:    6 ASEVRSTFINFFREKKEHTYVHSSSVIPHDDPTLLFANAGMNQFKPLFLGIADPNSDLAKLKRAVNTQKCIRAGGKHNDLDDVGKDVYHHTYFEMLGNWSFGDYFKKEIITWAWELLTTVYGIPAERLYVSVFGGDEANGVPADSEARDIWRSVGVPDERILNFGMKDNFWEMGDVGPCGPCSEIHYDRIGNRDASHLVNADDPMVVEIWNLVFIQFNREEGGVLKPLPAKHIDCGLGLERLIAVMQDKTSNYDTDIFQPIFEAIHKGSGVRAYTGFIGDEDKDGVDMAYRVVADHVRTLTIALSDGGRPDNSGRGYVLRRILRRGVRYASEKLNAQPGFFASLVPVVISILGETFPELSRDPVTVMDIINDEEKQFLKTLSRGRVLFQRAVQSLPEGTMTFPGDVSWRLYDTYGFPADLTQLMAEEKGLSVDNTAFEEARRKAIETSSAGTGKFRDTLDLDVHALAELQQKGVPTTDDSPKYAYTFTGEGSDAVYKFEPCVGKILAIRRDGKFVDQLAAGEEGAILLDRTNFYAEQGGQIYDVGVLTKVNDESNEFNVSNCQVRGGYIVLVGSAEGSFSVGDQVNERFDEDRKQLIMKNHTGTHVLNYALRKVL-ADSDQKGSLVAPDRMRFDFTNKAGMTVQQVKKAEEYAQQLIDTKGQVYAKNSPLGEAKKVKGLRAMFDETYPDPVRVVAVGTPVEQLLQNPDAEEGQNTTVEFCGGTHLQNVSHIGRIVIASEEAIAKGIRRIVALTGPEAERAIARADRLTARLEEESKHADKKDELLANKDKFKALQKKIQEIVDEANGAQLPYWRKDSIREKAKAIQKTLDGYTKAQQAAVAEKVLGEAKELAAVAEQPTVLVHVFAANANSKAIDNALKLLK---DTKAVMAFSVNEDSGKVLCLAKVDKSLVSNGLKANEWVNEV 927          

BLAST of alanine--trna cytoplasmic vs. SwissProt
Match: gi|74586350|sp|Q5A8K2.1|SYA_CANAL (RecName: Full=Alanine--tRNA ligase; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS; Flags: Precursor)

HSP 1 Score: 931.398 bits (2406), Expect = 0.000e+0
Identity = 484/946 (51.16%), Postives = 624/946 (65.96%), Query Frame = 0

Query:   48 STAGTMPPPT-----QAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAK-PGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDR---TIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDK--IALDVHAITEL-QDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVENE--ECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGS-VEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNL-AFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQ 977
            S+  T+P P       A +VR  FLD+F + + H Y  SSS +P +DPTLLFAN+GM Q+KPIF+G++DP SD A L RA NSQKCIRAGGKHNDL+DVG+D YHHTFFEMLGNWSFGDYFKKE   ++WE LT+V  L +DRLYVTYFGGD    LEPDLE K FWL +GVPE  ILPGS++DNFWEMGD GPCGPCSEIH+DRIGGRNA+ LVNMDDP+VLE+WN+VF+QYNRE D +LR LP KHID GMGFERLVSILQN  SNYDTDVF P+F+ I++ TGVR Y GK G+ED D +D AYRVIADH+RTLT A+ DGG P+N GRGYVLRRILRR  R+  + ++    GFF  LVDVV+E     FPE+    Q +K+I++EEE  F KTL RG KL ++     +K PE T  L G   WRLYDTYGFPVDLT+LM+EE GLK+D E +E AK+ ++ AS+G SGT + K  + LDVHA++EL Q+  +  TND               KY   + + KV+G+     FVD +E+   + G+LLDKT FYAE GGQ +D G +V   D   E  V NVQV AG+VLHTG+ V+G L VGD +    DE RR  + NNHTGTHILNFALR  L    DQ+GSLVAP++LRFDF+  +A+T ++++KIE +SN+ I  N++VY K+ SL  AK I GLRAVF ETYPDPVRVVS+G+ V+ L ADPTN      S+EFCGGTH+ ++G I   ++  E  IAKG+RRI+A+TG +A    + A   +++++               KE+   +  L       +IS   K  +      + K+I D  +A++     + ++     L+   N  +FLV  +   A  K +  A+  +K    +      + + D  K+     V+ E I +G+ ANE  +
Sbjct:   10 SSNTTIPTPNGSNHWTASKVRSTFLDYF-KKQQHTYVPSSSVVPHNDPTLLFANAGMNQYKPIFLGTVDPASDFASLKRAANSQKCIRAGGKHNDLEDVGRDSYHHTFFEMLGNWSFGDYFKKEAIDYSWELLTKVYGLQEDRLYVTYFGGDEKQGLEPDLEAKNFWLKVGVPEDHILPGSVEDNFWEMGDQGPCGPCSEIHYDRIGGRNASALVNMDDPNVLEVWNVVFIQYNREADGNLRTLPNKHIDTGMGFERLVSILQNKYSNYDTDVFLPIFDKIREITGVRPYTGKFGNEDKDGIDTAYRVIADHVRTLTFAICDGGVPNNEGRGYVLRRILRRGSRYVRKYMNYPIGGFFQQLVDVVIEQNKEIFPEISSGAQDLKEILNEEELSFAKTLDRGEKLFEQYAIIASKTPEQT--LSGKDVWRLYDTYGFPVDLTRLMAEEAGLKIDEEGFERAKEESREASKG-SGTKDGKTLVKLDVHALSELDQNDAIPKTND-------------EFKYGLENVKAKVVGIYDGSKFVDSIEDPSIQYGILLDKTPFYAEQGGQEYDTGKLVI--DGKSEFNVANVQVYAGYVLHTGNIVDGKLNVGDEIIATYDELRRWPIRNNHTGTHILNFALREVLGDGVDQKGSLVAPEKLRFDFSHKQAVTAKELEKIEAISNKYIKNNDKVYYKDVSLTKAKEINGLRAVFGETYPDPVRVVSIGVSVDDLLADPTNTKWHEISIEFCGGTHVAKTGDIKDLVIIEESGIAKGIRRIVAVTGHDAHHVQKVANEFEQEIDNASSLPFGVAKESKSKELGVALKKL-------SISVLDKQRLTEKFNKLDKSIKDNLKAKQKEETKKTLDVVNNWLNDKENASSFLVAHVPITANAKAITEAINLIKKQDKTKSIYLLTGETD--KVAHGCYVSDEAIAKGINANELAK 927          

BLAST of alanine--trna cytoplasmic vs. SwissProt
Match: gi|313104284|sp|P40825.3|SYA_YEAST (RecName: Full=Alanine--tRNA ligase, mitochondrial; AltName: Full=Alanyl-tRNA synthetase; Short=AlaRS; Flags: Precursor)

HSP 1 Score: 924.85 bits (2389), Expect = 0.000e+0
Identity = 463/930 (49.78%), Postives = 625/930 (67.20%), Query Frame = 0

Query:   59 AHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPG-FFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEG-TKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQ-GISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVE--NEECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSV-EGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVK---ALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQC 979
            A  VR  FLD+F ++K H +  SS  +P DDPTLLFAN+GM Q+KPIF+G++DP SD   L RA NSQKCIRAGGKHNDL+DVGKD YHHTFFEMLGNWSFGDYFKKE  T++W  LTEV  +PKDRLYVTYF GD    LEPD E ++ W ++GVP+  ILPG+ +DNFWEMGD GPCGPCSEIH+DRIGGRNAA LVNMDDPDVLE+WNLVF+Q+NREQD SL+ LP KHID GMGFERLVS+LQ+ RSNYDTDVF PLFE IQ+ T VR Y+G  G+ D D +D AYRV+ADH+RTLT AL+DGG P+N GRGYVLRRILRR  R+A + ++   G FFS L   ++  +   FPE+ KDP  + +I+DEEE  F KTL RG +L ++  +   +  +K L G   WRLYDTYGFPVDLT+LM+EE+GLK+D   +E AKQ +  AS+ G      D I L+VH ++EL D  V  TND               KY   + +G ++ L     FVD++    ++ G++LDKTCFYAE GGQ +D G +V   D++ E  V NVQ+  GFV HTGS+ EG L VGD +    DE RR  + NNHTGTHILNFAL+ +L  + DQ+GSLVAP++LRFDF+  KA++ E++KK+E + N+ I +N +V+ KE  L +AK+I G+RAVF ETYPDPVRVVSVG P+E+L A+P N   +  S+EFCGGTH+ ++G I +F++  E  IAKG+RRI+A+TG EA +A + A+     ++   K   SP   + +K++ +L   L +     +IS   K+E++     ++K + D+ ++R      + ++  K    +N N  +LV  ++     K +  A+  +K   ++   +  +   +DP+ G++     ++   + +G+  +   ++ 
Sbjct:   36 ATNVRNTFLDYF-KSKEHKFVKSSPVVPFDDPTLLFANAGMNQYKPIFLGTVDPASDFYTLKRAYNSQKCIRAGGKHNDLEDVGKDSYHHTFFEMLGNWSFGDYFKKEAITYSWTLLTEVYGIPKDRLYVTYFEGDEKLGLEPDTEARELWKNVGVPDDHILPGNAKDNFWEMGDQGPCGPCSEIHYDRIGGRNAASLVNMDDPDVLEVWNLVFIQFNREQDGSLKPLPAKHIDTGMGFERLVSVLQDVRSNYDTDVFTPLFERIQEITSVRPYSGNFGENDKDGIDTAYRVLADHVRTLTFALADGGVPNNEGRGYVLRRILRRGARYARKYMNYPIGNFFSTLAPTLISQVQDIFPELAKDPAFLFEILDEEEASFAKTLDRGERLFEKYASAASKTESKTLDGKQVWRLYDTYGFPVDLTELMAEEQGLKIDGPGFEKAKQESYEASKRGGKKDQSDLIKLNVHELSELNDAKVPKTND-------------EFKYGSANVEGTILKLHDGTNFVDEITEPGKKYGIILDKTCFYAEQGGQEYDTGKIVI--DDAAEFNVENVQLYNGFVFHTGSLEEGKLSVGDKIIASFDELRRFPIKNNHTGTHILNFALKETLGNDVDQKGSLVAPEKLRFDFSHKKAVSNEELKKVEDICNEQIKENLQVFYKEIPLDLAKSIDGVRAVFGETYPDPVRVVSVGKPIEELLANPANEEWTKYSIEFCGGTHVNKTGDIKYFVILEESGIAKGIRRIVAVTGTEAFEAQRLAEQFAADLDAADKLPFSP---IKEKKLKELGVKLGQ----LSISVITKNELKQKFNKIEKAVKDEVKSRAKKENKQTLDEVKTFFETNENAPYLVKFIDISPNAKAITEAINYMKSNDSVKDKSIYLLAGNDPE-GRVAHGCYISNAALAKGIDGSALAKKV 941          

BLAST of alanine--trna cytoplasmic vs. nr
Match: gi|1325276704|ref|XP_023323002.1| (alanine--tRNA ligase, cytoplasmic-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 1375.15 bits (3558), Expect = 0.000e+0
Identity = 657/926 (70.95%), Postives = 767/926 (82.83%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGG-RNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVEN-EECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWC 976
            M     A  +R+ FLDFF++ K H Y HSSSTIP DDPTLLFAN+GM QFKP+FVG+ DPNSDMAK +R  N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTW+WE LTEV +L   RLYVTYFGGD  + L+PDLE KQ WLDLGV E++ILPG+M+DNFWEMGDTGPCGPCSEIH+DRIGG R+AAHLVNMDDPDVLEIWNLVFMQYNREQDKSL+ LP KHIDCGMGFERLVS++Q+ RSNYDTD+F P F+ IQ GTGVRAYAG++G ED D +DMAYRV+ADH RTLTIALSDGGRPDNVGRGYVLRRILRR +R+A+EKL AKPGFF+ LV  VV++LG TFPEVKKDPQT+ DII+EEETQFLKTLSRG+KLLDRTI+KL EG K LPGDIAWRLYDTYGFPVDLTQLM EERGL+VD+ +YE  K AAQLASQG     ED I+LDVH+I EL++K  +PT+D  KY Y+AES    ++Y F +C  K++G+R  K FVD V + +ECGVLLD+TCFYAE GGQI+DEGF+V  ++   E+KV NVQVR G+VLH G+VEG+LK+GD + L +DE RRKNVMNNHTGTHILNFALR  L ++ADQ+GSLVAPD+LRFDFT+  A++  QVK +E + N +I KN+E+YAKEASLA+AK IQGLRAVFDETYPDPVRVVSVG+PV  LE DP NPAG+ TSVEFCGGTHLRR+GHI H ++ASEEAIAKG+RRI+ALTGPEA KA+ K +LL  ++E+VK  +ES    M  KE V+LIT+L +DI+AATISY KKDEMR  LK VKK IDD+DR RKAA+M + VE  K +L+ NP L +LV EL A A NK +DGALKQVK L+ +TP +FFS D DTGKILCMA  +KE I  GLKANEWC
Sbjct:    1 MDTSLDAKAIRKIFLDFFIEKKTHIYVHSSSTIPHDDPTLLFANAGMNQFKPVFVGTADPNSDMAKWTRVVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWSWELLTEVFKLDPARLYVTYFGGDEASGLQPDLEAKQLWLDLGVGESRILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGGRDAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLKQLPNKHIDCGMGFERLVSVVQDKRSNYDTDLFLPFFQHIQAGTGVRAYAGRVGAEDTDGIDMAYRVLADHARTLTIALSDGGRPDNVGRGYVLRRILRRGVRYASEKLGAKPGFFASLVHTVVQVLGETFPEVKKDPQTVIDIINEEETQFLKTLSRGQKLLDRTISKL-EGAKELPGDIAWRLYDTYGFPVDLTQLMCEERGLQVDMNSYESCKAAAQLASQGKGAGAEDTISLDVHSINELKEKSFQPTDDSFKYAYEAESSSKDSRYKFHACTAKILGIRHSKQFVDSVSSGQECGVLLDRTCFYAEQGGQIYDEGFLVNGEN---EMKVNNVQVRGGYVLHMGTVEGTLKLGDTVELSVDEERRKNVMNNHTGTHILNFALRQVLSSDADQKGSLVAPDKLRFDFTSKAAMSPAQVKNVEEIVNNVINKNQEIYAKEASLAVAKTIQGLRAVFDETYPDPVRVVSVGVPVVDLEKDPNNPAGTQTSVEFCGGTHLRRAGHIEHMVIASEEAIAKGIRRIVALTGPEAKKALAKEKLLLGELEKVKTKVESKD--MGMKEKVRLITELNDDISAATISYHKKDEMRGELKNVKKRIDDEDRNRKAAVMGDVVEAAKAILTGNPGLPYLVCELQAYANNKAIDGALKQVKLLSSATPTIFFSGDEDTGKILCMAYSSKEAITSGLKANEWC 920          

BLAST of alanine--trna cytoplasmic vs. nr
Match: gi|1325276702|ref|XP_023323001.1| (alanine--tRNA ligase, cytoplasmic-like isoform X1 [Eurytemora affinis])

HSP 1 Score: 1372.84 bits (3552), Expect = 0.000e+0
Identity = 657/926 (70.95%), Postives = 767/926 (82.83%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGG-RNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVEN-EECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWC 976
            M     A  +R+ FLDFF++ K H Y HSSSTIP DDPTLLFAN+GM QFKP+FVG+ DPNSDMAK +R  N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTW+WE LTEV +L   RLYVTYFGGD  + L+PDLE KQ WLDLGV E++ILPG+M+DNFWEMGDTGPCGPCSEIH+DRIGG R+AAHLVNMDDPDVLEIWNLVFMQYNREQDKSL+ LP KHIDCGMGFERLVS++Q+ RSNYDTD+F P F+ IQ GTGVRAYAG++G ED D +DMAYRV+ADH RTLTIALSDGGRPDNVGRGYVLRRILRR +R+A+EKL AKPGFF+ LV  VV++LG TFPEVKKDPQT+ DII+EEETQFLKTLSRG+KLLDRTI+KL EG K LPGDIAWRLYDTYGFPVDLTQLM EERGL+VD+ +YE  K AAQLASQG     ED I+LDVH+I EL++K  +PT+D  KY Y+AES    ++Y F +C  K++G+R  K FVD V + +ECGVLLD+TCFYAE GGQI+DEGF+V  ++   E+KV NVQVR G+VLH G+VEG+LK+GD + L +DE RRKNVMNNHTGTHILNFALR  L ++ADQ+GSLVAPD+LRFDFT+  A++  QVK +E + N +I KN+E+YAKEASLA+AK IQGLRAVFDETYPDPVRVVSVG+PV  LE DP NPAG+ TSVEFCGGTHLRR+GHI H ++ASEEAIAKG+RRI+ALTGPEA KA+ K +LL  ++E+VK  +ES    M  KE V+LIT+L +DI+AATISY KKDEMR  LK VKK IDD+DR RKAA+M + VE  K +L+ NP L +LV EL A A NK +DGALKQVK L+ +TP +FFS D DTGKILCMA  +KE I  GLKANEWC
Sbjct:    1 MDTSLDAKAIRKIFLDFFIEKKTHIYVHSSSTIPHDDPTLLFANAGMNQFKPVFVGTADPNSDMAKWTRVVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWSWELLTEVFKLDPARLYVTYFGGDEASGLQPDLEAKQLWLDLGVGESRILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGGRDAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLKQLPNKHIDCGMGFERLVSVVQDKRSNYDTDLFLPFFQHIQAGTGVRAYAGRVGAEDTDGIDMAYRVLADHARTLTIALSDGGRPDNVGRGYVLRRILRRGVRYASEKLGAKPGFFASLVHTVVQVLGETFPEVKKDPQTVIDIINEEETQFLKTLSRGQKLLDRTISKL-EGAKELPGDIAWRLYDTYGFPVDLTQLMCEERGLQVDMNSYESCKAAAQLASQGKGAGAEDTISLDVHSINELKEKSFQPTDDSFKYAYEAESSSKDSRYKFHACTAKILGIRHSKQFVDSVSSGQECGVLLDRTCFYAEQGGQIYDEGFLVNGEN---EMKVNNVQVRGGYVLHMGTVEGTLKLGDTVELSVDEERRKNVMNNHTGTHILNFALRQVLSSDADQKGSLVAPDKLRFDFTSKAAMSPAQVKNVEEIVNNVINKNQEIYAKEASLAVAKTIQGLRAVFDETYPDPVRVVSVGVPVVDLEKDPNNPAGTQTSVEFCGGTHLRRAGHIEHMVIASEEAIAKGIRRIVALTGPEAKKALAKEKLLLGELEKVKTKVESKD--MGMKEKVRLITELNDDISAATISYHKKDEMRGELKNVKKRIDDEDRNRKAAVMGDVVEAAKAILTGNPGLPYLVCELQAYANNKAIDGALKQVKLLSSATPTIFFSGDEDTGKILCMAYSSKEAITSGLKANEWC 920          

BLAST of alanine--trna cytoplasmic vs. nr
Match: gi|1339053358|ref|XP_023715037.1| (alanine--tRNA ligase, cytoplasmic isoform X1 [Cryptotermes secundus])

HSP 1 Score: 1347.03 bits (3485), Expect = 0.000e+0
Identity = 631/931 (67.78%), Postives = 754/931 (80.99%), Query Frame = 0

Query:   50 AGTMPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQV-ENEECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQC 979
            A  M     A EVR+AF+DFF+Q K H Y HSSSTIPLDDPTLLFAN+GM QFKPIF+G++DPNSDMAK  R  N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEIC WAWE LT+V +LP DRLYVTYFGGD  A LEPD EC++ W++LG+  + ILPGSM+DNFWEMG+TGPCGPCSE+HFDRIGGRNAAHLVN DDPDVLEIWNLVF+Q+NRE D SL+ LP+KHIDCGMG ERLVS++Q  RSNYDTD+F PLF AIQKGTGV  Y G +G++D D +DMAYRV+ADH RTLTIALSDGG PDN GRGYVLRRILRRA+R+ATEKL+AKPGFF+ LV+ VVE+LG TFPEVKKDPQ++ D+I+EEETQFLKTL+RG  LL+RTI KL   +K LPGD+AWRLYDTYGFPVDLTQLMSEE+GL VD++AYE AK+ AQ+ SQG  G  +DKI LD+HAITEL+D+ V  T+D PKY Y A+S++  A+Y F  C  KV+ LR  K FVD V   +ECGVLLDKT FYAE GGQI+DEGF+VK  DE++E  V NVQ+R G++LH G+VEG L  GD ++L LD  RR+ VMNNHTGTH+LNFALR+ L  EADQ+GSLVAPDRLRFDFT   A+++EQVK  E  SN +I KNE VYAKEASLA+AKAIQGLRAVF+ETYPDPVRVVS+GIPVEKLE DP  PAG++TSVEFCGGTHL R+GH+G F++ASEEAIAKG+RRI+ALTGPEA KA++K +LL+ ++ +    + S    ++ K++VK I +LT+D++ ATI YWKK+EMR NLK +KK +DD+DRA KA +  + +E  K LL+ NP    +V EL A +  K LDGALKQVKAL+  T AMFFS D +  KI+C++ V K  + +GLKANEW QQ 
Sbjct:   10 AKIMNTSLTAKEVRKAFIDFFIQ-KQHEYVHSSSTIPLDDPTLLFANAGMNQFKPIFLGTVDPNSDMAKWVRTVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICAWAWELLTQVFKLPSDRLYVTYFGGDRAAGLEPDEECREIWINLGLAPSHILPGSMKDNFWEMGETGPCGPCSELHFDRIGGRNAAHLVNEDDPDVLEIWNLVFIQFNRESDGSLKSLPKKHIDCGMGLERLVSVIQEKRSNYDTDLFMPLFAAIQKGTGVPPYKGHVGEDDVDGVDMAYRVLADHARTLTIALSDGGHPDNTGRGYVLRRILRRAVRYATEKLNAKPGFFATLVNTVVEILGDTFPEVKKDPQSVIDLINEEETQFLKTLTRGHNLLNRTIMKLGN-SKTLPGDVAWRLYDTYGFPVDLTQLMSEEKGLTVDMDAYEEAKKQAQILSQGRGGWYDDKIILDIHAITELRDQNVPLTDDSPKYNYHAKSEDKDAEYEFDGCIAKVLRLRHSKKFVDHVTSGQECGVLLDKTSFYAEQGGQIYDEGFLVKVGDENVEFSVKNVQIRGGYILHIGTVEGILCEGDEVSLHLDTSRRRLVMNNHTGTHVLNFALRTVLGTEADQKGSLVAPDRLRFDFTNKGAMSVEQVKATEVHSNAVIDKNEPVYAKEASLAVAKAIQGLRAVFEETYPDPVRVVSIGIPVEKLEEDPYGPAGNNTSVEFCGGTHLHRAGHVGKFVIASEEAIAKGIRRIVALTGPEASKALKKTELLENKLNKFISLLNSDDSEISNKDLVKKIVELTDDVSHATIPYWKKEEMRTNLKHLKKQLDDKDRASKAEVTAKVLEEVKALLAGNPKAPVIVQELKAYSNAKALDGALKQVKALSAETSAMFFSVDSEAKKIVCLSAVPKSAVNRGLKANEWVQQV 938          

BLAST of alanine--trna cytoplasmic vs. nr
Match: gi|1339053360|ref|XP_023715038.1| (alanine--tRNA ligase, cytoplasmic isoform X2 [Cryptotermes secundus] >gi|1330898256|gb|PNF25968.1| Alanine--tRNA ligase, cytoplasmic [Cryptotermes secundus])

HSP 1 Score: 1346.64 bits (3484), Expect = 0.000e+0
Identity = 630/928 (67.89%), Postives = 753/928 (81.14%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQV-ENEECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQC 979
            M     A EVR+AF+DFF+Q K H Y HSSSTIPLDDPTLLFAN+GM QFKPIF+G++DPNSDMAK  R  N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEIC WAWE LT+V +LP DRLYVTYFGGD  A LEPD EC++ W++LG+  + ILPGSM+DNFWEMG+TGPCGPCSE+HFDRIGGRNAAHLVN DDPDVLEIWNLVF+Q+NRE D SL+ LP+KHIDCGMG ERLVS++Q  RSNYDTD+F PLF AIQKGTGV  Y G +G++D D +DMAYRV+ADH RTLTIALSDGG PDN GRGYVLRRILRRA+R+ATEKL+AKPGFF+ LV+ VVE+LG TFPEVKKDPQ++ D+I+EEETQFLKTL+RG  LL+RTI KL   +K LPGD+AWRLYDTYGFPVDLTQLMSEE+GL VD++AYE AK+ AQ+ SQG  G  +DKI LD+HAITEL+D+ V  T+D PKY Y A+S++  A+Y F  C  KV+ LR  K FVD V   +ECGVLLDKT FYAE GGQI+DEGF+VK  DE++E  V NVQ+R G++LH G+VEG L  GD ++L LD  RR+ VMNNHTGTH+LNFALR+ L  EADQ+GSLVAPDRLRFDFT   A+++EQVK  E  SN +I KNE VYAKEASLA+AKAIQGLRAVF+ETYPDPVRVVS+GIPVEKLE DP  PAG++TSVEFCGGTHL R+GH+G F++ASEEAIAKG+RRI+ALTGPEA KA++K +LL+ ++ +    + S    ++ K++VK I +LT+D++ ATI YWKK+EMR NLK +KK +DD+DRA KA +  + +E  K LL+ NP    +V EL A +  K LDGALKQVKAL+  T AMFFS D +  KI+C++ V K  + +GLKANEW QQ 
Sbjct:    1 MNTSLTAKEVRKAFIDFFIQ-KQHEYVHSSSTIPLDDPTLLFANAGMNQFKPIFLGTVDPNSDMAKWVRTVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICAWAWELLTQVFKLPSDRLYVTYFGGDRAAGLEPDEECREIWINLGLAPSHILPGSMKDNFWEMGETGPCGPCSELHFDRIGGRNAAHLVNEDDPDVLEIWNLVFIQFNRESDGSLKSLPKKHIDCGMGLERLVSVIQEKRSNYDTDLFMPLFAAIQKGTGVPPYKGHVGEDDVDGVDMAYRVLADHARTLTIALSDGGHPDNTGRGYVLRRILRRAVRYATEKLNAKPGFFATLVNTVVEILGDTFPEVKKDPQSVIDLINEEETQFLKTLTRGHNLLNRTIMKLGN-SKTLPGDVAWRLYDTYGFPVDLTQLMSEEKGLTVDMDAYEEAKKQAQILSQGRGGWYDDKIILDIHAITELRDQNVPLTDDSPKYNYHAKSEDKDAEYEFDGCIAKVLRLRHSKKFVDHVTSGQECGVLLDKTSFYAEQGGQIYDEGFLVKVGDENVEFSVKNVQIRGGYILHIGTVEGILCEGDEVSLHLDTSRRRLVMNNHTGTHVLNFALRTVLGTEADQKGSLVAPDRLRFDFTNKGAMSVEQVKATEVHSNAVIDKNEPVYAKEASLAVAKAIQGLRAVFEETYPDPVRVVSIGIPVEKLEEDPYGPAGNNTSVEFCGGTHLHRAGHVGKFVIASEEAIAKGIRRIVALTGPEASKALKKTELLENKLNKFISLLNSDDSEISNKDLVKKIVELTDDVSHATIPYWKKEEMRTNLKHLKKQLDDKDRASKAEVTAKVLEEVKALLAGNPKAPVIVQELKAYSNAKALDGALKQVKALSAETSAMFFSVDSEAKKIVCLSAVPKSAVNRGLKANEWVQQV 926          

BLAST of alanine--trna cytoplasmic vs. nr
Match: gi|1059401830|ref|XP_017773819.1| (PREDICTED: alanine--tRNA ligase, cytoplasmic [Nicrophorus vespilloides])

HSP 1 Score: 1342.02 bits (3472), Expect = 0.000e+0
Identity = 620/919 (67.46%), Postives = 751/919 (81.72%), Query Frame = 0

Query:   59 AHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVE-NEECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWC 976
            A E+R +F+DFF + K H Y HSSSTIPLDDPTLLFAN+GM Q+KPIF+G++DPNSDM+K  RA N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEIC WAWE LT V +LP DRLYVTYFGGD  + L+PD ECKQ W+DLG+  + +LPG+M+DNFWEMG+TGPCGPCSE+HFDRIG R   HLVNMDDPDVLEIWNLVF+QYNRE D SL+ LP+KHIDCG+G ERLVS++QN RSNYDTD+F PLFEAIQKGTG  AY G++GDED D +DMAYRV+ADH RTLTIALSDGG PDN GRGYVLRRILRRA+R+ATEKL+AKPGFF+ LV+ VVE+LG TFPEVKKDPQ+I D I+EEE QFLKTL RGR LL+RTI KL + +K LPGD+AWRLYDTYGFPVDLTQLM+EE+GL VD+++YE +K+ AQLASQG    VED I LDVHAIT++Q+KG+ PT+D PKY Y   S++ +A+Y F  C+  +I +R +K F ++V   +ECG++LDKTCFYAE GGQI+D G+MVK  DES+E  V NVQVR G+++H GSVEG+LKVGD L L LD+ RR+ VMNNHTGTH+LN+ALR  L  EADQRGSLVAPDRLRFDFT   ALTI+QVKK E  SN++I KN+EV+AKE++L +AK I+GLRAVF+ETYPDPVR+VS+G PVEKLE+DP  PAG +TS+EFCGGTHL+ +GHIG F++ASEEAIAKG+RRI+ALTGPEA KA++K +LL+  ++E+   I + K     KE VK I +LTE+I+ A I YW+KDE+RN LK +KK +DD++RA KAA+  + V+  KQL++  PN   LV EL A +  K LD ALKQVK L+P T A+FFS DPD+GKI C++ V K    +GLKANEW 
Sbjct:    7 AKEIRASFIDFF-KNKKHEYVHSSSTIPLDDPTLLFANAGMNQYKPIFLGTVDPNSDMSKWVRAVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICAWAWELLTVVFKLPSDRLYVTYFGGDEKSGLKPDDECKQIWIDLGIKPSHVLPGNMKDNFWEMGETGPCGPCSELHFDRIGNREVPHLVNMDDPDVLEIWNLVFIQYNRETDGSLKSLPKKHIDCGLGLERLVSVIQNKRSNYDTDLFGPLFEAIQKGTGAPAYQGRVGDEDVDGVDMAYRVLADHARTLTIALSDGGNPDNTGRGYVLRRILRRAVRYATEKLNAKPGFFATLVNTVVEILGDTFPEVKKDPQSIMDTINEEEEQFLKTLLRGRNLLNRTIGKLGD-SKTLPGDVAWRLYDTYGFPVDLTQLMAEEKGLNVDMDSYEESKKQAQLASQGKGSGVEDTINLDVHAITDMQNKGIPPTDDSPKYNYTV-SNDLNAEYSFAPCEASIISIRYNKQFENEVSTGQECGIILDKTCFYAEQGGQIYDTGYMVKIGDESVEFSVKNVQVRGGYIMHIGSVEGTLKVGDKLALNLDKSRRRLVMNNHTGTHVLNYALRKVLGTEADQRGSLVAPDRLRFDFTNKGALTIDQVKKTELSSNELISKNDEVFAKESNLGVAKTIKGLRAVFEETYPDPVRIVSIGTPVEKLESDPFGPAGENTSIEFCGGTHLKYAGHIGDFVIASEEAIAKGIRRIVALTGPEAAKALKKNELLEASVKEINNLITADKSGSKSKEYVKKIVELTEEISHAIIPYWRKDELRNELKNLKKALDDKERAGKAAVANKVVDEIKQLVTEKPNQPILVKELKAFSNTKALDSALKQVKTLSPETSAIFFSVDPDSGKIFCLSSVPKSAQEKGLKANEWV 922          

BLAST of alanine--trna cytoplasmic vs. nr
Match: gi|998517755|ref|XP_015519721.1| (PREDICTED: alanine--tRNA ligase, cytoplasmic [Neodiprion lecontei])

HSP 1 Score: 1334.32 bits (3452), Expect = 0.000e+0
Identity = 621/921 (67.43%), Postives = 747/921 (81.11%), Query Frame = 0

Query:   59 AHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQV-ENEECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSV-EGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQ 977
            A E+RQAF+DFF ++K H Y HSS+TIP DDPTLLFAN+GM QFKPIF+G++DPNSDMAK  R  N+QKCIRAGGKHNDLDDVGKDVYHHTFFEM+GNWSFGDYFKKEIC WAWE LTEV +LP DRLYVTYFGGD  + L+PD ECKQ WL LGVP + +LPGSM+DNFWEMG+TGPCGPCSE+HFDRIGGR   HLVNMDDPDVLEIWNLVF+Q+NRE D SLR LP+KHIDCG+G ERLVS++Q  R+NYDTD+F PLF+ IQKGTG R Y GK+G ED D +DMAYRV+ADH RT+TIAL+DGG PDN GRGYVLRRILRRA+R+ATEKL+AKPGFF  LV+ VV+LLG  FPEV+KDPQ+I D+I+EEE QFLKTLSRGR LL+RT+AKL E T V+PGD+AWRLYDTYGFPVDLTQLM+EE+GL +D++ YE AK+ AQL SQG +G V+D+I LDVHAITELQ++GVKPTND  KY YKA+S +  A+Y F SC G VI LR  K FV+QV   +E GVLLD+T FYAE GGQI+DEGF+VK  DES E+ + NVQVR G+VLH G+V EG LK GD +NL +D  RR+ +MNNHTGTH LN+ALR+ L  EADQ+GSLVAPDRLRFDFT   A+T+EQVK+ E  + Q+I +N  +YAKE+ LAIAK IQGLRA+F+ETYPDPVR++S+GIPVE+LE +P + AG+ TSVEFCGGTHL  +GHIG F++ASEEAIAKG+RRI+ALTGPEA KA++K +LL+ ++ ++K  IE+ K  +  KE VK I DLTED++ A I YWKKDEMRN LK +KK+IDD++RA KAA+    V+ T  L+  N  +A LV  L A +  K LD ALKQVK+L+P T A+FFS D D  KI  ++ V K  + +GLKANEW Q
Sbjct:    7 AKEIRQAFIDFF-KSKNHEYVHSSATIPHDDPTLLFANAGMNQFKPIFLGTVDPNSDMAKWVRVVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMMGNWSFGDYFKKEICAWAWELLTEVFKLPSDRLYVTYFGGDEQSGLKPDEECKQIWLSLGVPPSHVLPGSMKDNFWEMGETGPCGPCSELHFDRIGGREVPHLVNMDDPDVLEIWNLVFIQFNRESDGSLRSLPKKHIDCGLGLERLVSVIQGKRANYDTDLFMPLFDMIQKGTGARTYQGKVGSEDTDGIDMAYRVLADHARTITIALADGGMPDNTGRGYVLRRILRRAVRYATEKLNAKPGFFGSLVNTVVDLLGDVFPEVRKDPQSIIDVINEEEQQFLKTLSRGRNLLNRTMAKL-EATNVVPGDVAWRLYDTYGFPVDLTQLMTEEKGLTIDMKGYEEAKKQAQLLSQGKAGGVDDQINLDVHAITELQNQGVKPTNDSHKYNYKAKSSDKDAEYEFASCVGTVIALRFAKQFVEQVTSGQEVGVLLDQTNFYAEQGGQIYDEGFLVKVGDESTEVCIQNVQVRGGYVLHIGTVGEGILKKGDKVNLNVDTARRRLIMNNHTGTHALNYALRTVLGWEADQKGSLVAPDRLRFDFTNKGAMTVEQVKQTEINTKQLIQRNNPIYAKESGLAIAKTIQGLRAMFEETYPDPVRIISIGIPVEELEKNPLSTAGTKTSVEFCGGTHLHHAGHIGDFVIASEEAIAKGIRRIVALTGPEATKALKKVELLENELNKIKSIIEADKTGVKSKEHVKAIVDLTEDVSHAIIPYWKKDEMRNTLKTLKKSIDDKERAAKAAVANLVVQKTADLIKRNVGVAVLVELLEAYSNTKALDSALKQVKSLSPETSALFFSVDNDAKKIFALSAVPKSAVAKGLKANEWIQ 925          

BLAST of alanine--trna cytoplasmic vs. nr
Match: gi|1227089133|gb|OXU16738.1| (hypothetical protein TSAR_010539 [Trichomalopsis sarcophagae])

HSP 1 Score: 1331.24 bits (3444), Expect = 0.000e+0
Identity = 623/921 (67.64%), Postives = 758/921 (82.30%), Query Frame = 0

Query:   59 AHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVEN-EECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSV-EGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQ 977
            A ++RQA++DFF ++K H Y HSSSTIP DDPTLLF N+GM QFKPIF+G++DPNSDMAK  R  NSQKCIRAGGKHNDLDDVGKDVYHHTFFEM+GNWSFGDYFKKEIC WAWEFLT+   LP DRLYVTYFGGD  +NL+PD ECK FWL LGVPE+ +LPGSM+DNFWEMG+TGPCGPCSE+H+DRIGGR AAHLVNMDDPDVLEIWNLVF+QYNREQ+  L+ LP+KHIDCG+G ERLVS++QN RSNYDTD+F PLF+AIQKGTG  AY G++G++D D +DMAYRV+ADH RT+TIAL+DGG PDN GRGYV+RRILRRA+R+ATEKL+A+PGFF+ L++VVVELLG  FPEVK+DPQ+I DI++EEE QFLKTLSRGR LL+RTIAKL E TKV+PGD+AWRLYDTYGFPVDLTQLM+EE+GL +D+ AYE AK+ A +ASQG +G V D+I LDVHAITELQ+KGVK T+D PKY YK E  E  A+Y F  C G V+ LRKDKTFVD+V++ +E GVLLD+T FYAE GGQI+DEGF+VK DDES E++VTNVQVR G+VLH G+V EG+LK GD L L +D  RR+ +M+NHT TH LN ALRS L  +ADQ+GSLVAPDRLRFDFT   A+T EQVKK E  +N ++ +++E+YAKE+ LAIA+ IQGLRA+F ETYPDPVRVVS+G+ VE+LE DP +PAG+ TSVEFCGGTHL R+GHIG F++ASEEAIAKG+RRI+ALTGPEA KA++KA+LLQ  + ++K ++E+ K + T KE+VK I +LT+D++ A I YWKK+EMR+ LKA+KK +DD++R+ KAA+    VE    ++ SN     LV  LNA    K LD ALK+VKA++P T A+F S D D  KI  ++ V K  I +GLKANEW Q
Sbjct:    6 AKDIRQAYIDFF-KSKDHEYVHSSSTIPHDDPTLLFTNAGMNQFKPIFLGTVDPNSDMAKWVRVVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMMGNWSFGDYFKKEICEWAWEFLTQQLNLPADRLYVTYFGGDEKSNLKPDEECKNFWLSLGVPESHVLPGSMKDNFWEMGETGPCGPCSELHYDRIGGREAAHLVNMDDPDVLEIWNLVFIQYNREQNGELQPLPKKHIDCGLGLERLVSVIQNKRSNYDTDLFTPLFDAIQKGTGAPAYQGRVGNDDKDGIDMAYRVLADHARTITIALADGGVPDNTGRGYVIRRILRRAVRYATEKLNAQPGFFATLINVVVELLGDVFPEVKRDPQSIIDIVNEEEVQFLKTLSRGRNLLNRTIAKL-ESTKVVPGDVAWRLYDTYGFPVDLTQLMAEEKGLTIDVAAYEEAKKLAIIASQGKAGGVNDQINLDVHAITELQNKGVKTTDDSPKYNYKVEKTEKYAEYTFAPCTGTVLALRKDKTFVDEVKSGDEVGVLLDQTNFYAEQGGQIYDEGFLVKVDDESTEIRVTNVQVRGGYVLHIGTVGEGTLKKGDKLYLNVDTSRRRLIMSNHTATHALNHALRSVLGTDADQKGSLVAPDRLRFDFTNKGAMTAEQVKKTEQFTNLMVKEDKEIYAKESPLAIARTIQGLRAMFGETYPDPVRVVSMGVTVEELEKDPLSPAGAKTSVEFCGGTHLHRTGHIGDFVIASEEAIAKGIRRIVALTGPEASKALKKAELLQNNLNQIKSSVETVKDSATSKEIVKKIVELTDDVSHAVIPYWKKEEMRSELKALKKALDDKERSAKAAVANAVVETAVNIIESNKGAPVLVEVLNAFNNTKALDAALKKVKAISPETSALFISVDEDEKKIFALSAVPKSAIAKGLKANEWIQ 924          

BLAST of alanine--trna cytoplasmic vs. nr
Match: gi|91084029|ref|XP_966465.1| (PREDICTED: alanine--tRNA ligase, cytoplasmic [Tribolium castaneum] >gi|642924795|ref|XP_008194044.1| PREDICTED: alanine--tRNA ligase, cytoplasmic [Tribolium castaneum] >gi|270006700|gb|EFA03148.1| Alanine--tRNA ligase, cytoplasmic-like Protein [Tribolium castaneum])

HSP 1 Score: 1327.77 bits (3435), Expect = 0.000e+0
Identity = 618/926 (66.74%), Postives = 749/926 (80.89%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVE-NEECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQ 977
            M     A +VR A+++FF + K H+Y HSSSTIPLDDPTLLF N+GM QFKPIF+G++DPNSD+AKL+RA NSQKCIRAGGKHNDLDDVGKDVYHHTFFEM+GNWSFGDYFKKEIC WAWEFLT+   LP DRLYVTYFGGD  A LEPD ECKQ WL LGVPE+ +LPGSM+DNFWEMG+TGPCGPCSE+HFDRIGGR+   LVN DDPDVLEIWNLVF+QYNRE D SL+ LP+KHIDCG+G ERLVSI+QN RSNYDTD+F PLF+AIQKGTG   Y GK+GD D D +DMAYRV+ADH RT+TIALSDGG PDN GRGYVLRRILRRA+R+ATEKL+AKPGFFS LV+ VVE+LG TFPEV KDPQ+I D I+EEE QFLKTLSRGR LL+RTI KL + +K LPGD+AWRLYDTYGFPVDLTQLM+EE+GL VD+  YE +K+ AQ+ASQG    VED I LDVHAITELQ+ GV PT+D PKY Y A S   + +Y F  C   VI LR +K FV+QV   +ECG+LLDKT FYAE GGQI+D G++VK  DES+E  V NVQVR G+V+H GS+EG+LK GD + L +D  RR+ +MNNHTGTH+LN+ALR  L  EADQRGSLVAPDRLRFDFT   A+T++QVK  E  S ++I +N++V+AKE++LA+AK I+GLRAVF+ETYPDPVR+VS+GIPVEKLE DP  PAG +TS+EFCGGTHL+ SGHIG F++ASEEAIAKG+RRI+ALTGPEA KA+++ + L+  + ++K  +E+ K     KE+VK I  LTE+++ A I YWKK+E+RN LK +KK +DD++RA KAA+  + V+  K+ + +NPNL  LV EL A +  K LD ALKQV++L+PST A+F + DPD+ K+ C+A V+KE + +GLKANEW Q
Sbjct:    1 MDISMSAKDVRNAYINFFKE-KNHDYVHSSSTIPLDDPTLLFTNAGMNQFKPIFLGTVDPNSDLAKLTRAVNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMMGNWSFGDYFKKEICAWAWEFLTKRLHLPGDRLYVTYFGGDKAAGLEPDNECKQIWLGLGVPESHVLPGSMKDNFWEMGETGPCGPCSELHFDRIGGRDVPELVNQDDPDVLEIWNLVFIQYNRESDGSLKFLPKKHIDCGLGLERLVSIMQNKRSNYDTDLFLPLFDAIQKGTGAPPYGGKIGDADKDGIDMAYRVLADHARTITIALSDGGNPDNTGRGYVLRRILRRAVRYATEKLNAKPGFFSTLVNTVVEILGDTFPEVCKDPQSIIDTINEEEEQFLKTLSRGRNLLNRTITKLGD-SKTLPGDVAWRLYDTYGFPVDLTQLMAEEKGLNVDMAVYEESKKLAQIASQGKGSGVEDTINLDVHAITELQNLGVPPTDDAPKYNYIAGSG-INEEYKFELCHSTVIALRHNKQFVEQVHTGQECGILLDKTNFYAEQGGQIYDTGYIVKIGDESVEFSVKNVQVRGGYVIHIGSLEGTLKKGDKVALHVDSARRRLIMNNHTGTHVLNYALRKVLGTEADQRGSLVAPDRLRFDFTNKGAMTVDQVKNTELNSKELISRNDQVFAKESNLAVAKTIRGLRAVFEETYPDPVRIVSIGIPVEKLEVDPFGPAGDNTSIEFCGGTHLKYSGHIGDFVIASEEAIAKGIRRIVALTGPEATKALKRTEYLENCLNDIKFLVETDKNGEKSKELVKKIVQLTEEVSHAVIPYWKKEEIRNALKTLKKNLDDKERAAKAAVANQVVDQIKEFVKANPNLPILVKELKAFSNTKALDTALKQVRSLSPSTSALFVTVDPDSNKMFCLASVSKEGVDKGLKANEWVQ 923          

BLAST of alanine--trna cytoplasmic vs. nr
Match: gi|1000738379|ref|XP_015591734.1| (PREDICTED: alanine--tRNA ligase, cytoplasmic [Cephus cinctus] >gi|1000738381|ref|XP_015591735.1| PREDICTED: alanine--tRNA ligase, cytoplasmic [Cephus cinctus] >gi|1000738383|ref|XP_015591736.1| PREDICTED: alanine--tRNA ligase, cytoplasmic [Cephus cinctus] >gi|1000738385|ref|XP_015591737.1| PREDICTED: alanine--tRNA ligase, cytoplasmic [Cephus cinctus])

HSP 1 Score: 1326.23 bits (3431), Expect = 0.000e+0
Identity = 615/927 (66.34%), Postives = 752/927 (81.12%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVEN-EECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSV-EGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQ 977
            M     A+E+RQA++DFF ++K H Y HSSSTIP DDPTLLFAN+GM QFKPIF+G++DPNSDM+K  R  N+QKCIRAGGKHNDLDDVGKDVYHHTFFEM+GNWSFGDYFKKEICTWAWE LT V +LP ++LYVTYFGGD  A LEPD ECK  WL LG+  + +LPGSM+DNFWEMG+TGPCGPCSE+HFDRIGGR AAHLVNMDDPDVLEIWNLVF+Q+NRE D  LR LP+KHIDCG+G ERL+S++QN R+NYDTD+F PLFEAIQKGTG  AY G++G +D D +DMAYRV+ADH RT+TIAL+DGG PDN GRGYVLRRILRRA+R+ATEKL+AKPGFFS LV+VVVELLG  FPEV+KDPQ+I DII+EEE QFLKTLSRGR LL+RTIAKL E TK++PGD+AWRLYDTYGFPVDLTQLM+EE+GL VD+ AYE AK+ AQ+ SQG +G V+D+I LDVHAITELQ KG+KPTNDLPKY Y  +S++ +A+Y F  C   VI LR+ K FVD+V + EE GVLLD+TCFYAE GGQI+DEGF+VK  DE  E++V NVQVR G+VLH G+V +G LK GD ++  +D  RR+ +M+NHTGTH LNFALR  L  EADQ+GSLVAPDRLRFDFT   A+T  QVK+ E  +N +I +NE +YAKE++L++AK IQGLRA+F+ETYPDPVRVVS+G+PVE+LE DP +PAG+ TS+EFCGGTHL  +GHIG F++ASEEAIAKG+RRI+ALTGPEA KA++K QLLQ Q+ +++  I++ K     K+ VK I +LTED++ A I YWKKDE+RN+LKA+KK +DD++RA K A+    VE  ++++ +N  +  LV  L A +  K LD ALKQVK ++P T A+F S D D  KI  ++ V K  + +GLKANEW Q
Sbjct:    1 MNSSMTANEIRQAYIDFF-KSKGHEYVHSSSTIPHDDPTLLFANAGMNQFKPIFLGTVDPNSDMSKWVRVVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMMGNWSFGDYFKKEICTWAWELLTSVLKLPANQLYVTYFGGDKKAGLEPDNECKDIWLSLGLLPSHVLPGSMKDNFWEMGETGPCGPCSELHFDRIGGREAAHLVNMDDPDVLEIWNLVFIQFNRETDGGLRPLPKKHIDCGLGLERLISVIQNKRANYDTDLFIPLFEAIQKGTGAPAYQGRVGADDKDGIDMAYRVLADHARTITIALADGGVPDNTGRGYVLRRILRRAVRYATEKLNAKPGFFSSLVNVVVELLGDVFPEVRKDPQSIIDIINEEEDQFLKTLSRGRNLLNRTIAKL-EKTKIVPGDVAWRLYDTYGFPVDLTQLMTEEKGLTVDMTAYEEAKKQAQILSQGKAGGVDDQINLDVHAITELQSKGIKPTNDLPKYNYTVKSNDKNAEYQFTPCTSTVIALRRAKQFVDEVSSGEEVGVLLDQTCFYAEQGGQIYDEGFLVKVGDEDTEIRVKNVQVRGGYVLHIGTVGQGKLKNGDKVHANIDTARRRLIMSNHTGTHALNFALRKVLGGEADQKGSLVAPDRLRFDFTNKGAMTPLQVKQSEEFTNDLIKRNEMIYAKESNLSVAKTIQGLRAMFEETYPDPVRVVSIGVPVEELEKDPFSPAGTKTSIEFCGGTHLHYTGHIGDFVIASEEAIAKGIRRIVALTGPEATKALKKTQLLQNQLNQIRNLIDNDKSGANSKDYVKKIVELTEDVSHAVIPYWKKDEIRNSLKALKKALDDKERAAKVAVANLVVEKVQKIIQANVGIPILVEVLEAYSNTKALDSALKQVKTISPETSALFLSVDQDANKIFALSAVPKTAVAKGLKANEWIQ 925          

BLAST of alanine--trna cytoplasmic vs. nr
Match: gi|1279725612|ref|XP_022907416.1| (alanine--tRNA ligase, cytoplasmic [Onthophagus taurus])

HSP 1 Score: 1323.92 bits (3425), Expect = 0.000e+0
Identity = 621/928 (66.92%), Postives = 735/928 (79.20%), Query Frame = 0

Query:   53 MPPPTQAHEVRQAFLDFFMQTKAHNYAHSSSTIPLDDPTLLFANSGMCQFKPIFVGSIDPNSDMAKLSRACNSQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICTWAWEFLTEVCELPKDRLYVTYFGGDATANLEPDLECKQFWLDLGVPEAKILPGSMQDNFWEMGDTGPCGPCSEIHFDRIGGRNAAHLVNMDDPDVLEIWNLVFMQYNREQDKSLRILPRKHIDCGMGFERLVSILQNTRSNYDTDVFQPLFEAIQKGTGVRAYAGKLGDEDADKLDMAYRVIADHIRTLTIALSDGGRPDNVGRGYVLRRILRRAIRFATEKLSAKPGFFSGLVDVVVELLGPTFPEVKKDPQTIKDIIDEEETQFLKTLSRGRKLLDRTIAKLPEGTKVLPGDIAWRLYDTYGFPVDLTQLMSEERGLKVDIEAYEVAKQAAQLASQGISGTVEDKIALDVHAITELQDKGVKPTNDLPKYEYKAESDEPSAKYVFGSCQGKVIGLRKDKTFVDQVEN-EECGVLLDKTCFYAEAGGQIFDEGFMVKADDESIELKVTNVQVRAGFVLHTGSVEGSLKVGDVLNLQLDEGRRKNVMNNHTGTHILNFALRSSLEAEADQRGSLVAPDRLRFDFTANKALTIEQVKKIETVSNQIIGKNEEVYAKEASLAIAKAIQGLRAVFDETYPDPVRVVSVGIPVEKLEADPTNPAGSSTSVEFCGGTHLRRSGHIGHFIVASEEAIAKGVRRIIALTGPEAVKAIQKAQLLQKQMEEVKKTIESPKCTMTQKEMVKLITDLTEDINAATISYWKKDEMRNNLKAVKKTIDDQDRARKAALMTECVEFTKQLLSSNPNLAFLVLELNACAQNKVLDGALKQVKALAPSTPAMFFSSDPDTGKILCMAQVAKETIGQGLKANEWCQQC 979
            M     A E+R AF++FF ++K H Y HSSSTIPLDDPTLLF N+GM QFKPIF+G  DPNSDMAK +R  N+QKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEIC WAWE LTEV +LP +RLYVTYFGGD  + LEPDLECKQ WL+LG+    I+PG+M+DNFWEMG+TGPCGPCSE+HFDRIG R+  HLVN DDPDVLEIWNLVF+Q+NRE D SL++LP+KHIDCG+G ERLVS++QN RSNYDTD+FQPLFEAIQKGTG   Y G++GDED D +DMAYRV+ADH RTLTIALSDGG PDN GRGYVLRRILRRA+R+ATEKL A+P FF+ LV+ VVELLG TFPEVKKDPQ I D+I+EEE QFLKTLSRGR LL+RTI KL  G   LPGD+AWRLYDTYGFPVDLTQLM+EE+GL VD+  YE +K+ AQL SQG    +ED I LDVH+ITELQ KGV PT+D PKY Y A S   S  Y F  C+ KVI LR +K FVD +   +E G+LLDKTCFYAE GGQI+D G++VK DDES+E  V NVQVR G+++H G++EG LKVGD +NLQ+D  RR+ VMNNHTGTHILN+ALR  L  EADQRGSLVAPDRLRFDFT   AL  EQVK++E  S +II  N+EV+AKE +L +AK I GLRAVF+ETYPDPVRVVSVGIPVE LE DP  P G+ TSVEFCGGTHL+  GH G F+++SEEAIAKG+RRI+ALTGPEA KA++K +LL+ ++ ++K TI+  K     KE+VK I +LTE+ + + I YWKK+E+RN LK +KK +DD++RA+KAA+  + V+  K  L  NPNL  +V  LNA +  K LD ALKQVKAL+P + AMF S D D  K+  +A V KE   +GLKANEW QQ 
Sbjct:    1 MNTEMTAKEIRSAFINFF-KSKKHEYVHSSSTIPLDDPTLLFTNAGMNQFKPIFLGIADPNSDMAKWTRTVNTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGNWSFGDYFKKEICAWAWELLTEVLKLPSERLYVTYFGGDEKSGLEPDLECKQIWLELGIKPEHIIPGNMKDNFWEMGETGPCGPCSELHFDRIGNRDVPHLVNQDDPDVLEIWNLVFIQFNRESDGSLKLLPKKHIDCGLGLERLVSVIQNKRSNYDTDLFQPLFEAIQKGTGAPKYQGRVGDEDKDGIDMAYRVLADHARTLTIALSDGGSPDNTGRGYVLRRILRRAVRYATEKLHAQPKFFASLVNTVVELLGETFPEVKKDPQGIIDVINEEEEQFLKTLSRGRNLLNRTITKLGGGCNNLPGDVAWRLYDTYGFPVDLTQLMAEEKGLAVDMNGYEESKKQAQLISQGKGIGIEDTINLDVHSITELQQKGVSPTDDSPKYNYSATS--TSFDYTFEPCEAKVIALRFNKQFVDSITTGQEAGILLDKTCFYAEQGGQIYDTGYIVKTDDESVEFTVKNVQVRGGYIIHIGNIEGVLKVGDKVNLQIDSSRRRLVMNNHTGTHILNYALRKVLGTEADQRGSLVAPDRLRFDFTNKGALNAEQVKQVELASKEIIATNKEVFAKETALGVAKTINGLRAVFEETYPDPVRVVSVGIPVETLETDPFGPEGTKTSVEFCGGTHLKYVGHCGDFVISSEEAIAKGIRRIVALTGPEAAKALKKTELLENKLNDIKTTID--KSDFDSKEIVKSIVELTEEASHSIIPYWKKEEIRNALKTLKKNLDDKERAKKAAIANQVVDEVKLFLKENPNLPIIVKCLNAASNTKALDSALKQVKALSPKSAAMFVSVDMDCSKVFSLAAVPKEAQEKGLKANEWIQQV 923