BLAST of Beta-mannosidase vs. L. salmonis genes
Match: EMLSAG00000003286 (supercontig:LSalAtl2s:LSalAtl2s17:851625:866671:1 gene:EMLSAG00000003286 transcript:EMLSAT00000003286 description:"snap_masked-LSalAtl2s17-processed-gene-8.1")

HSP 1 Score: 293.123 bits (749), Expect = 5.236e-90
Identity = 187/505 (37.03%), Postives = 288/505 (57.03%), Query Frame = 0

Query:  230 WDIGPSFPSSGIYRPIW-IQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEG-IEGDLGVEIHDIESGEPL--FGTTASVVSELFPEKGALNEFVVEASI-FEDK-VKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVD-HWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNGKIDRGQVGQDPGSPF---FGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQ-EADLY-FPSRFLNHRQHGHGSNDEMVSTASEHF---ETDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKD-GRGMTMGALYWQLN 718
            WD GP++PS GI++P++ I +     I  + ++T L     +  W+ KI+ II ++   + G L +++ ++   + L   G T S + E       L+E  +  ++ F++K V+ WWPNG G+Q LY   V   S+   +S K I + FR V+L+Q+       ++   G +F F++N + +F KGSN +P H LPE + +  +  LLE  +K++MNM+RVWGGG+Y+SD FY+ AD +GI+IWQDFMFACS+YP+  +FL +V++EV+ QV RLQ+H S+      W   N N   +  +W N+ +N S + +DY  LY +TI+  V  E  +  +V SSPSNG      + +D  SP+   +GD HFY+     W+ + YP  R ASEYGFQS  S+  L + ++ + DL    S F   R+H  G   ++ S    +    E+ L    ++  G+ + IY +Q+ QA  L VETE + RS     +    G TMGALYWQLN
Sbjct:   20 WDWGPAYPSMGIWKPLYLILSNDYISINSIKWKTSL----KRNTWRVKIQTIILTQSSYLTGKLQLKMAELGIAKTLEVGGNTDSSIFE------GLSELKINTTMEFDEKSVQMWWPNGYGQQKLYNFCVTFFSNTSKVS-KEIDVAFRSVKLVQE-------AITPKGLSFMFQVNGQNIFAKGSNWIPSHILPENLEQDRIIHLLEGVKKANMNMLRVWGGGVYESDRFYKLADSMGILIWQDFMFACSLYPSDTDFLIDVKEEVIQQVRRLQYHASIAI----WAGNNENEAALSGNWYNTLSNFSIFKDDYNKLYGQTIQNTVHIEDFSRPFVKSSPSNGD-GPSSLEKDSVSPYNSIYGDTHFYDYYSRFWDSKSYPSTRFASEYGFQSVSSLEDLNKVTEPDTDLKNVNSNFFVSREHHLGGLIQLKSQIESYLGLEESQL----KNRHGVERFIYFSQIYQAIGLKVETEHYLRSKYTYDESTSEGFTMGALYWQLN 497          

BLAST of Beta-mannosidase vs. SwissProt
Match: gi|81878533|sp|Q8K2I4.1|MANBA_MOUSE (RecName: Full=Beta-mannosidase; AltName: Full=Lysosomal beta A mannosidase; AltName: Full=Mannanase; Short=Mannase; Flags: Precursor)

HSP 1 Score: 488.804 bits (1257), Expect = 1.611e-160
Identity = 280/673 (41.60%), Postives = 399/673 (59.29%), Query Frame = 0

Query:   55 PKHDLAGSDWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIK--IIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFVVEASIFED-KVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNG--KIDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFE----TDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            P + L+GS W+V N NGS+   A+VPG ++S L + G++ +  Y+RFND+NY WI  +NWTYS  F IP ++ ++  + L  DG+DTVAEI FN+  +GKT+NMF  YSFDI   ++ V  N+L ++F+S V YA  + + H+   Y VPPE  P++  GE H NFIRK Q SF WD GPSFPS GI++ + I+A+  A ++YLTF    V D   + W  +IK    + S + + G + V I       P   T  +   EL  E+  +   V    I +D  V+ WWP G G Q  Y + +    DG +   K   + FR VQLI++ +K       S G +FYFKIN   +F+KGSN +P  +  ++V    +  L +    ++MN +RVWGGG+Y+ D FY   DELGIM+WQDFMFA ++YPT   FL +VR+EV +QV RL+ HPS++  S N  N N   + V+ +  +  +   Y +DY+ LY K I+KIV  E  +  ++ SSP+NG   ++ G +  DP S  +GDIHFY  A + WN +I+P  RL SEYG+QS+PS  TL++ S + D  + SRF  HRQH    N +M+     HF+    TD +   +D+      IYL QV QA+ +  ETE + RS + +  DG+G TMGALYWQLN
Sbjct:   21 PSYSLSGS-WRVSNGNGSLELPATVPGYVHSALHQHGLIQDP-YYRFNDLNYRWISLDNWTYSTEFKIPFNLSEWQKVKLIFDGVDTVAEILFNNVTIGKTDNMFTGYSFDITNVVKDV--NSLKLQFRSAVQYAECQSKAHTS--YRVPPECPPVEQKGECHVNFIRKAQCSFSWDWGPSFPSQGIWKDVRIEAYNIAHLDYLTFLP--VYDNASQAWNIEIKASFDVASSKSVGGQVTVAI-------PQLKTQQTNDIELQQEQRIVKLLV---KIRKDVAVETWWPRGHGNQTGYNMTILFALDGGLKIEKAAKVYFRTVQLIEEGIKG------SPGLSFYFKINGLPIFLKGSNWIPADSFQDKVTSDRLQLLFQSVVDANMNTLRVWGGGIYEQDEFYALCDELGIMVWQDFMFASALYPTEPGFLASVRKEVTYQVRRLKSHPSIIIWSGN--NENEVALSVNWFHVNPRDMKTYIDDYVTLYVKNIRKIVLSEDKSRPFIASSPTNGMKTMEEGWISYDPYSIQYGDIHFYNYADDCWNWKIFPKARLVSEYGYQSWPSFSTLEKVSSQEDWAYNSRFSLHRQHHEDGNHQMLHQVKMHFKLPQGTDPLRTFKDT------IYLTQVMQAQCIKTETEFYLRSRSEIV-DGKGHTMGALYWQLN 660          

BLAST of Beta-mannosidase vs. SwissProt
Match: gi|51338734|sp|O00462.3|MANBA_HUMAN (RecName: Full=Beta-mannosidase; AltName: Full=Lysosomal beta A mannosidase; AltName: Full=Mannanase; Short=Mannase; Flags: Precursor)

HSP 1 Score: 486.108 bits (1250), Expect = 1.595e-159
Identity = 269/665 (40.45%), Postives = 402/665 (60.45%), Query Frame = 0

Query:   63 DWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKII--IQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFV-VEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNGK--IDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFE----TDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            +W + N NGS+    +VPG ++S L + G++ +S Y+RFND+NY W+  +NWTYSK F IP  + K+  + L L+G+DTV++I FN+  +G+T+NMF RYSFDI   ++ V  N++ ++FQS VLYA  + + H++  Y VPP+  P+   GE H NF+RK Q SF WD GPSFP+ GI++ + I+A+    + Y TF    + D+  ++W  +I+    + S + + G + V I       P   T  +   EL P K  +  FV +  +I    V+ WWP+G G Q  Y + V    DG +   K   + FR V+LI++P+K       S G +FYFKIN   +F+KGSN +P  +  +RV    +  LL+    ++MN +RVWGGG+Y+ D FY+  DELGIM+WQDFMFAC++YPT + FL +V  EV +Q+ RL+ HPS++  S N  N N   + ++ +  S+ +   Y +DY+ LY K I+++V     +  ++TSSP+NG   +    V Q+P S +FGD+HFY+   + WN +++P  R ASEYG+QS+PS  TL++ S   D  F S+F  HRQH  G N +M+  A  HF+    TD +   +D+      IYL QV QA+ +  ETE +RRS + +  D +G TMGALYWQLN
Sbjct:   28 NWSICNGNGSLELPGAVPGCVHSALFQQGLIQDS-YYRFNDLNYRWVSLDNWTYSKEFKIPFEISKWQKVNLILEGVDTVSKILFNEVTIGETDNMFNRYSFDITNVVRDV--NSIELRFQSAVLYAAQQSKAHTR--YQVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNICHLNYFTFSP--IYDKSAQEWNLEIESTFDVVSSKPVGGQVIVAI-------PKLQTQQTYSIELQPGKRIVELFVNISKNI---TVETWWPHGHGNQTGYNMTVLFELDGGLNIEKSAKVYFRTVELIEEPIKG------SPGLSFYFKINGFPIFLKGSNWIPADSFQDRVTSELLRLLLQSVVDANMNTLRVWGGGIYEQDEFYELCDELGIMVWQDFMFACALYPTDQGFLDSVTAEVAYQIKRLKSHPSIIIWSGN--NENEEALMMNWYHISFTDRPIYIKDYVTLYVKNIRELVLAGDKSRPFITSSPTNGAETVAEAWVSQNPNSNYFGDVHFYDYISDCWNWKVFPKARFASEYGYQSWPSFSTLEKVSSTEDWSFNSKFSLHRQHHEGGNKQMLYQAGLHFKLPQSTDPLRTFKDT------IYLTQVMQAQCVKTETEFYRRSRSEIV-DQQGHTMGALYWQLN 660          

BLAST of Beta-mannosidase vs. SwissProt
Match: gi|115502255|sp|Q4FZV0.1|MANBA_RAT (RecName: Full=Beta-mannosidase; AltName: Full=Lysosomal beta A mannosidase; AltName: Full=Mannanase; Short=Mannase; Flags: Precursor)

HSP 1 Score: 473.781 bits (1218), Expect = 1.106e-154
Identity = 272/675 (40.30%), Postives = 395/675 (58.52%), Query Frame = 0

Query:   55 PKHDLAGSDWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFVVEASIFEDKVK--------KWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNG--KIDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFETDLIVHQRDS-DGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            P + L GS W+V N N S+   A+VPG ++S L++ G++ +  Y+RFND+NY WI  +NWTYS  F IP +  ++  + L  DG+DTVAEI FN+  +GKT+NMF RYSFD+   ++ V  N+L ++FQS V YA  + + H++  Y VPPE  P++  GE H NFIRK Q SF WD GPSFPS GI++ + I+A+  A +++LTF    + D   + W  +I+                  D+ S +P+ G     + EL  ++    E      I +  VK         WWP+G G Q  Y   +    DG +   K   + FR VQLI++P+        S G +FYFKIN   +F+KGSN +P  +  ++V    +  LL+ A  ++MN +RVWGGG+Y+ D FY   DELGIM+WQDFMFA ++YPT   FL +VR+EV +QV RL+ HPSV+  S N  N N   + V+ +  +  +   Y  DY+ LY KTI++IV  E  +  ++ SSP+NG   +  G + +DP S  +GD+HFY+   + W+ +++P  RL SEYG+QS+PS  TL++ S++ D  + SRF  HRQH    N+EM+     HF+   +  +RD        IYL QV QA+ +  ETE + RS + +  +G G TMGALYWQLN
Sbjct:   21 PSYSLRGS-WRVSNGNSSLELPATVPGYVHSALQQHGLIQDP-YYRFNDLNYRWISLDNWTYSTEFKIPFNRSEWQKVKLIFDGVDTVAEILFNNVTIGKTDNMFTRYSFDVTNVVKDV--NSLKLRFQSAVQYAECQSKAHTQ--YRVPPECPPVEQKGECHVNFIRKEQCSFSWDWGPSFPSQGIWKDVRIEAYNIAHLDHLTFLP--LYDNTSQAWTIEIEASF---------------DVVSTKPVGGQVTIAIPELKTQQANHIELQHGQRIVKLLVKIRKDVTVETWWPHGHGNQTGYNTTILFALDGGLKIEKAAKVYFRTVQLIEEPITG------SPGLSFYFKINGLPIFLKGSNWIPADSFQDKVTSELLQLLLQSAVDANMNTLRVWGGGIYEQDEFYALCDELGIMVWQDFMFASALYPTEPGFLESVRKEVTYQVRRLKSHPSVIIWSGN--NENEVALRVNWFHVNPRDLGTYINDYVTLYVKTIREIVLSEDRSRPFIASSPTNGVKTMTEGWISKDPYSTQYGDMHFYDYFSDCWDWKVFPKARLVSEYGYQSWPSFSTLQKVSRQEDWSYSSRFSLHRQHHGNGNNEMLHQVQLHFQ---LPQRRDPVRAFKDTIYLTQVMQAQCIKTETEFYLRSRSEIV-NGEGHTMGALYWQLN 660          

BLAST of Beta-mannosidase vs. SwissProt
Match: gi|3024103|sp|Q29444.1|MANBA_BOVIN (RecName: Full=Beta-mannosidase; AltName: Full=Lysosomal beta A mannosidase; AltName: Full=Mannanase; Short=Mannase; Flags: Precursor)

HSP 1 Score: 467.618 bits (1202), Expect = 2.087e-152
Identity = 265/665 (39.85%), Postives = 380/665 (57.14%), Query Frame = 0

Query:   63 DWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFVVEASIFEDK---VKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNGK--IDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFE----TDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            +WK+ + NGS+   A+VPG ++S L    I+ +  Y+RFN+++Y WI  +NWTY K F +   M  +S + L  +GIDTVA +  N   +GKT+NMF RYSFDI   ++ V  N + V+FQSPV+YA  + E H+   Y VPP   P   +GE H NFIRK Q SFGWD GPSFP+ GI++ + I+A+    + Y  F    + D   + W  KI          E    V    + SGE +       + +    +    E  VE  +  DK   V+ WWP+G G Q  Y + V    DG +   K   + FR V+L+++P++       S G +FYFKIN   +F+KGSN +P  +  +RV    +  LL+    ++MN +RVWGGG+Y+ D FY+  DELGIMIWQDFMFAC++YPT K+F+ +VR+EV HQV RL+ HPS++  S N  N N   + +  +         Y +DY+ LY K I+ IV +   T  ++TSSP+NG   I  G +  +P    +GD+HFY+   + WN + +P  R  SEYG+QS+PS  TL++ S E D  + S F  HRQH    N+EM+     HF+    TD +   +D+      +YL QV QA+ +  ETE +RRS + +  +G+G TMGALYWQLN
Sbjct:   28 NWKIHSGNGSLQLPATVPGCVHSALFNKRIIKDP-YYRFNNLDYRWIALDNWTYIKKFKLHSDMSTWSKVNLVFEGIDTVAVVLLNSVPIGKTDNMFRRYSFDITHTVKAV--NIIEVRFQSPVVYANQRSERHTA--YWVPPNCPPPVQDGECHVNFIRKMQCSFGWDWGPSFPTQGIWKDVRIEAYNVCHLNYFMFTP--IYDNYMKTWNLKI----------ESSFDVVSSKLVSGEAIVAIPELNIQQTNNIELQHGERTVELFVKIDKAIIVETWWPHGHGNQTGYNMSVIFELDGGLRFEKSAKVYFRTVELVEEPIQN------SPGLSFYFKINGLPIFLKGSNWIPADSFQDRVTSAMLRLLLQSVVDANMNALRVWGGGVYEQDEFYELCDELGIMIWQDFMFACALYPTDKDFMDSVREEVTHQVRRLKSHPSIITWSGN--NENEAALMMGWYDTKPGYLQTYIKDYVTLYVKNIRTIVLEGDQTRPFITSSPTNGAKTIAEGWLSPNPYDLNYGDVHFYDYVSDCWNWRTFPKARFVSEYGYQSWPSFSTLEKVSSEEDWSYRSSFALHRQHLINGNNEMLHQIELHFKLPNSTDQLRRFKDT------LYLTQVMQAQCVKTETEFYRRSRSEIV-NGKGHTMGALYWQLN 660          

BLAST of Beta-mannosidase vs. SwissProt
Match: gi|3024109|sp|Q95327.1|MANBA_CAPHI (RecName: Full=Beta-mannosidase; AltName: Full=Lysosomal beta A mannosidase; AltName: Full=Mannanase; Short=Mannase; Flags: Precursor)

HSP 1 Score: 462.611 bits (1189), Expect = 1.961e-150
Identity = 263/664 (39.61%), Postives = 375/664 (56.48%), Query Frame = 0

Query:   63 DWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFVVEASIFEDK---VKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNGK--IDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFETDLIVHQRDSDGLAKL---IYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            +WK+ N NGS+   A+VPG ++S L    I+ +  Y+RFN+++Y WI  +NWTY K F +   M +++ + L  +GIDTVA +  N   +GKT+NMF RYSFDI   ++ V  N + V+FQSPV+YA  + E H+   Y VPP   P   +GE H NFIRK Q SFGWD GPSFP+ GI++ + I+A+    + Y  F    + D     W  KI          E    V    + SGE +       + +    +    E  V+  +  DK   V+ WWP+G G Q  Y + V    DG +   K   + FR V+L+++P++       S G TFYFKIN   +F+KGSN +P  +  +RV    +  LL+    ++MN +RVWGGG+Y+ D FY+  DELGIMIWQDFMFAC++YPT ++F+ +VR+EV HQV RL+ HPS++  S N  N N   + +  +         Y +DY+ LY K I+ IV +   T  ++ SSP+NG      G +  +P    +GD+HFY+   + WN + +P  R  SEYG+QS+PS  TL++ S E D  + S F  HRQH    N EM+     HF+         +D L +    +YL QV QA+ +  ETE +RRS N +  DG+G TMGALYWQLN
Sbjct:   28 NWKIHNGNGSLQLPAAVPGCVHSALFNKRIIKDP-YYRFNNLDYRWIALDNWTYIKKFKLHSDMSEWNKVNLVFEGIDTVAVVLLNSVPIGKTDNMFRRYSFDITHMVKAV--NIIEVRFQSPVIYANQRSERHTA--YWVPPNCPPPVQDGECHVNFIRKMQCSFGWDWGPSFPTQGIWKDVRIEAYNICHLNYFMFTP--IYDNYMETWNLKI----------ESSFDVVSSKLVSGEAIVAIPELNIQQRNNIELRHGERTVKLFVKIDKAVIVETWWPHGHGNQTGYDMTVTFELDGGLRFEKSAKVYFRTVELVEEPIQN------SPGLTFYFKINGLPIFLKGSNWIPADSFQDRVTSDMLRLLLQSVVDANMNALRVWGGGIYEQDEFYELCDELGIMIWQDFMFACALYPTDEDFMDSVREEVTHQVRRLKSHPSIITWSGN--NENEAALMMGWYDTKPGYLHTYIKDYVTLYVKNIRTIVLEGDQTRPFIISSPTNGAKTTAEGWLSPNPYDLNYGDVHFYDYMSDCWNWRTFPKARFVSEYGYQSWPSFSTLEKVSSEEDWSYESSFALHRQHLINGNSEMLQQIELHFKL-----PNSADQLRRFKDTLYLTQVMQAQCVKTETEFYRRSRNEIV-DGKGHTMGALYWQLN 660          

BLAST of Beta-mannosidase vs. SwissProt
Match: gi|3024108|sp|Q93324.1|MANBA_CAEEL (RecName: Full=Probable beta-mannosidase; AltName: Full=Mannanase; Short=Mannase; Flags: Precursor)

HSP 1 Score: 374.4 bits (960), Expect = 1.784e-116
Identity = 235/680 (34.56%), Postives = 360/680 (52.94%), Query Frame = 0

Query:   58 DLAGSDWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNH-YIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKG--ALNEFVVEASI-FEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHAL-PERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNG--KIDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHF------------ETDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            +LAG +W+  ++N +++   +VPG IYSDL  +GI++  + F  N +N  WI  ++WTYS+ F +   +       L ++ +DT+A +  N  +V  + N F+ Y  ++ + I  + +N + +KF+S V YA  + +E+ K   + +PP+  P  Y+GE H NFIRK Q SF WD GPSFP+ GI   I I  ++        ++T         +WK   +           +  V+I       P  G   S    L   K     ++ ++  SI  E + ++WWPNGMGEQ LY + V +   G++   K   IGF+ V+L+Q  +    P  P  G  FYFKIN   VF+KG+N +P+     +R      + LL+   +  MN IRVWGGG Y+S++FY  A + GI++WQD MFAC++YPTT+EF+ N  +EV + V R+  H SV+  S N  N N   +    WK S    S+  +DY+ LY++ + KI KK APT  ++ SSPSNG    + G V ++P    +GDIH+Y    N W    Y  PR ASEYG QS+P   T+  +  E+D  + S+ + HRQH  G     +    +H             +     +   +  +++L Y +QV+Q+ +L  +T  +RR  N  T +G G TM A+YWQLN
Sbjct:   27 NLAG-NWEFSSSNKTVNGTGTVPGDIYSDLYASGIIDNPL-FGENHLNLKWIAEDDWTYSRKFRLI-DLDDTVGAFLEIESVDTIATVYVNGQKVLHSRNQFLPYHVNVTDIIA-LGENDITIKFKSSVKYAEKRADEYKKIFGHSLPPDCNPDIYHGECHQNFIRKAQYSFAWDWGPSFPTVGIPSTITINIYRGQYFHDFNWKTRF----AHGKWKVAFEFDTFHYGARTIEYSVQI-------PELGIKESDYYRLSATKSLQTRSKNIMSLSIPMEHEPERWWPNGMGEQKLYDVVVSM--GGQVKEKK---IGFKTVELVQDLID---PKKPEKGRNFYFKINDEPVFLKGTNWIPVSMFRSDRENIAKTEFLLDSVAEVGMNAIRVWGGGFYESNHFYYYASKKGILVWQDLMFACALYPTTEEFIQNAEEEVSYNVDRISQHTSVIVFSGN--NENEAAIRGHWWKASNYTESQQVKDYVLLYQR-LAKIAKKVAPTIPFIMSSPSNGVETEEEGGVSKNPYDVRYGDIHYYNEFVNLWRDDTYLTPRCASEYGVQSYPMKETMLNWINESDWEYTSKAMFHRQHHPGGIATNLLMIFQHLPIPAECGSKSVSDVPSCKYISSASYMSRLAYFSQVHQSIALKTQTLHYRRFRNTTTNEGLGNTMCAMYWQLN 680          

BLAST of Beta-mannosidase vs. SwissProt
Match: gi|298351836|sp|Q5B7W2.2|MANBB_EMENI (RecName: Full=Beta-mannosidase B; AltName: Full=Mannanase B; Short=Mannase B)

HSP 1 Score: 259.225 bits (661), Expect = 5.134e-74
Identity = 190/660 (28.79%), Postives = 308/660 (46.67%), Query Frame = 0

Query:   78 SVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFVVEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYL-------WLYEKTIKKIVKKEAPTTVWVTSSP-SNGKIDRGQVGQDPGSPFFGDIHFYERAQNQWNP--------QIYPI--PRLASEYGFQSFPSIHTLKQY-SQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFETDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            +VP  ++ DL+  G L++  +   N+++  W+  ++WTY  VF  P ++   S+I L  DG+DT A++  +   + +++NMF+ +  DI + +    ++TL + F   +L AR   ++H  + ++         +NG+     +RK Q  +GWD GP   ++GI++ + ++ + SAKI  L  E  L ED       SK +I   +E        VE     +      T +    ++  E   L+  V + +    +   WWPNG G+Q LY++ V L  +           G R  ++IQ+P K        +G +F+F+IN   +F  G+  +P  +L   +        +E     H  MIRVWGGG+Y+ + FYQ  DE+G+M+WQDFMF C  YPT  E L ++ +E  + + RL+HHPS+V     W   N +    +    +Y    +  E +L       ++YE  + K V+K  P+  +   SP  +GKI           P  GD+H       QWN         QI+     R  SE+G ++FP + T+  + + EAD Y  S  L+      G    +         T L+ + R +  L   IYL QV QAE++      +RR        G     GAL WQLN
Sbjct:   31 TVPSVVHQDLQANGKLDDP-FIGLNELSARWVNEKSWTYRNVFQKP-TVPAGSSIFLVFDGLDTFAKVKLDGQVILESDNMFLAHRVDITKALDVEGEHTLEIDFDCALLRARELRKQHPDHKWV--------GFNGDTARLSVRKAQYHWGWDWGPVLMTAGIWKEVRLEVY-SAKISDLWTEVHLAEDH------SKARITAAAE--------VETQGTGNSYKATFTLSLQGQQIGKEVATLDGNVAKTTFDVQEPSLWWPNGYGDQTLYEISVSLEKEEEQAHQVSKKFGIRTAEVIQRPDK--------HGKSFFFRINGVDIFCGGACWIPADSLLTNITPDRYRKWIELMAVGHQVMIRVWGGGIYEDESFYQACDEVGVMVWQDFMFGCGNYPTWPEILESIEKEAEYNLRRLRHHPSIVI----WVGNNEDYQVQEQQGLTYNYADKDPESWLKTDFPARYIYEHLLPKAVQKIIPSAYYHPGSPWGDGKI--------TSDPTVGDMH-------QWNVWHGTQEKYQIFDTLGGRFNSEFGMEAFPHMSTIDHFVTNEADKYPQSHVLDFHNKADGHERRIA--------TYLVENLRTATDLEVYIYLTQVVQAETMMFGYRGWRRQWGDERHCG-----GALLWQLN 625          

BLAST of Beta-mannosidase vs. SwissProt
Match: gi|298351731|sp|B8NW36.1|MANBB_ASPFN (RecName: Full=Beta-mannosidase B; AltName: Full=Mannanase B; Short=Mannase B)

HSP 1 Score: 256.914 bits (655), Expect = 4.231e-73
Identity = 189/656 (28.81%), Postives = 306/656 (46.65%), Query Frame = 0

Query:   79 VPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKI-IIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFVVEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKN---WYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSP-SNGKIDRGQVGQDPGSPFFGDIHFYERAQNQWNP--------QIYPI--PRLASEYGFQSFPSIHTLKQYSQ-EADLYFPSRFLNHRQHGHGSNDEMVSTASEHFETDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            VP   + DL+    L ++ Y  FN+++  W+  ++WTY  VF  P ++   S+I+L  DG+DT A +  +   + +++NMF+ +  D+ + ++    + L + F   +  AR   E+ +K+++          +NG+     +RK Q  +GWD GP   ++GI+R + ++ + SAKI  L  E +L  D    +  +  ++    S +  +    + +H                 E+  E   L + V + +    +   WWPNG G+  LY++ V L  +   + +    IG R  +LIQQP +        +G +F+F+IN   VF  GS  +P   L   +        +E        MIRVWGGG Y+ D FYQ  DELG+++WQDFMF C  YPT  E L ++ +E  + V RL+HHPS+V    N   +      G+  D+   +  N  +      ++YEK +  +V+K +P TV+   SP  +GKI           P  GD+H       QWN         QI+     R  SE+G ++FP + T++ + + EAD Y  S  L+      G    +         T L+ + R +  L   +YL QV QAE++      +RR        G     GAL WQLN
Sbjct:   32 VPSVAHQDLQANQKL-KNPYIGFNELDARWVNDKSWTYRTVFQKP-AVAAGSSIILAFDGLDTFATVKLDGSVILQSDNMFLAHRVDVTKALEAEGDHVLEIDFDCAMRRARELREKDTKHNW--------ASFNGDPARMAVRKAQYHWGWDWGPLLSTAGIWREVRLEVY-SAKISDLWTEVELASDHQTARVSAFTEVDAADSVDSYKASFLLSLHG---------------KEVAREVATLKDKVAKVTFDVTQPSLWWPNGYGDPALYEISVSLEKEDCEIHSVSKKIGIRTAELIQQPDR--------HGKSFFFRINGVDVFCGGSCWIPADNLLPSITAERYRKWIELMVAGRQVMIRVWGGGCYEDDSFYQACDELGVLVWQDFMFGCGNYPTWPELLESIEKEANYNVRRLRHHPSIVVYVGNNEDYQVQESAGLVYDYEDKNPENWLKTDFPARYIYEKLLPSVVEKLSPKTVYHPGSPWGDGKIT--------SDPTVGDMH-------QWNVWHGTQEKYQIFDTLGGRFNSEFGMEAFPHMSTIEYFVENEADKYPQSHVLDFHNKADGHERRIA--------TYLVENLRTATDLETYVYLTQVVQAETMMFGYRGWRRQWGDERHCG-----GALLWQLN 625          

BLAST of Beta-mannosidase vs. SwissProt
Match: gi|597439628|sp|Q2TXB7.3|MANBB_ASPOR (RecName: Full=Beta-mannosidase B; AltName: Full=Mannanase B; Short=Mannase B)

HSP 1 Score: 256.529 bits (654), Expect = 6.326e-73
Identity = 189/656 (28.81%), Postives = 305/656 (46.49%), Query Frame = 0

Query:   79 VPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKI-IIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFVVEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKN---WYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSP-SNGKIDRGQVGQDPGSPFFGDIHFYERAQNQWNP--------QIYPI--PRLASEYGFQSFPSIHTLKQYSQ-EADLYFPSRFLNHRQHGHGSNDEMVSTASEHFETDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            VP   + DL+    L ++ Y  FN+++  W+  ++WTY  VF  P ++   S+I+L  DG+DT A +  +   + +++NMF+ +  D+ + ++    + L + F   +  AR   E+ +K+++          +NG+     +RK Q  +GWD GP   ++GI+R + ++ + SAKI  L  E +L  D    +  +  ++    S +  +    + +H                 E+  E   L + V   +    +   WWPNG G+  LY++ V L  +   + +    IG R  +LIQQP +        +G +F+F+IN   VF  GS  +P   L   +        +E        MIRVWGGG Y+ D FYQ  DELG+++WQDFMF C  YPT  E L ++ +E  + V RL+HHPS+V    N   +      G+  D+   +  N  +      ++YEK +  +V+K +P TV+   SP  +GKI           P  GD+H       QWN         QI+     R  SE+G ++FP + T++ + + EAD Y  S  L+      G    +         T L+ + R +  L   +YL QV QAE++      +RR        G     GAL WQLN
Sbjct:   32 VPSVAHQDLQANQKL-KNPYIGFNELDARWVNDKSWTYRTVFQKP-AVAAGSSIILAFDGLDTFATVKLDGSVILQSDNMFLAHRVDVTKALEAEGDHVLEIDFDCAMRRARELREKDTKHNW--------ASFNGDPARMAVRKAQYHWGWDWGPLLSTAGIWREVRLEVY-SAKISDLWTEVELASDHQTARVSAFAEVDAADSVDSYKASFLLSLHG---------------KEVAREVATLKDKVANVTFDVTQPSLWWPNGYGDPALYEISVSLEKEDCEIHSVSKKIGIRTAELIQQPDR--------HGKSFFFRINGVDVFCGGSCWIPADNLLPSITAERYRKWIELMVAGRQVMIRVWGGGCYEDDSFYQACDELGVLVWQDFMFGCGNYPTWPELLESIEKEANYNVRRLRHHPSIVVYVGNNEDYQVQESAGLVYDYEDKNPENWLKTDFPARYIYEKLLPSVVEKLSPKTVYHPGSPWGDGKIT--------SDPTVGDMH-------QWNVWHGTQEKYQIFDTLGGRFNSEFGMEAFPHMSTIEYFVENEADKYPQSHVLDFHNKADGHERRIA--------TYLVENLRTATDLETYVYLTQVVQAETMMFGYRGWRRQWGDERHCG-----GALLWQLN 625          

BLAST of Beta-mannosidase vs. SwissProt
Match: gi|298351835|sp|Q0CCA0.2|MANBB_ASPTN (RecName: Full=Beta-mannosidase B; AltName: Full=Mannanase B; Short=Mannase B)

HSP 1 Score: 254.603 bits (649), Expect = 2.302e-72
Identity = 193/657 (29.38%), Postives = 304/657 (46.27%), Query Frame = 0

Query:   77 ASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFVVEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKN---WYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSP-SNGKIDRGQVGQDPGSPFFGDIHFYERAQNQWNP--------QIYPI--PRLASEYGFQSFPSIHTLKQYSQ-EADLYFPSRFLNHRQHGHGSNDEMVSTASEHFETDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            A VP  ++ DL     L +  Y  F +++  W+  ++WTY  VF  P ++   S+++L  DG+DT A++  N + + ++ NMF+ +  D+ + +     + L + F   +L AR   E  +K+     P+   + +NG+     +RK Q  +GWD GP   ++GI+R + ++ + +A++  L  ETDL  D    Q    I    Q E  I       I  +   E      A  V+E   +   +   V + S+       WWPNG G+  LY++   L  DG  +      IG R  +++Q+P K        +G +F+F+IN   +F  GS  +P   L   +        +E        MIRVWGGG Y+ D FYQ  DELG+M+WQDFMF C  YPT  E L +V +E ++ V RL+HHPS+V    N   +      G+  +       N  +      ++YEK + ++V++ +P+T +   SP  +GKI           P  GD+H       QWN         QI+     R  SE+G ++FP + T++ + + E D Y  S  L+      G    +         T L+ + R +  L   IYL QV QAE++      +RR        G     GAL WQLN
Sbjct:   30 AQVPSVVHQDLIANNKLQDP-YVGFRELDARWVNEKSWTYRTVFQKP-AVPAGSSVILAFDGLDTFAKVKLNGNVILESNNMFLAHRIDVTKALGADGDHVLEIDFDCAMLRAR---ELRAKD-----PQHKWVGFNGDPARMGVRKAQYHWGWDWGPVLMTAGIWRDVRLEVY-TARVADLWTETDLAVDHHAAQ----ISAFAQVEGAISSSKVNFILSLHGQE-----VARAVAEPQDQVAKVAFDVQQPSL-------WWPNGYGDPTLYEISATLDQDGATVHQISKKIGIRTAEVVQRPDK--------HGKSFFFRINGVDIFCGGSCWIPADNLLPSISAERYRKWIELMVHGRQVMIRVWGGGCYEDDSFYQACDELGVMVWQDFMFGCGNYPTWPEMLESVEKEAIYNVRRLRHHPSIVVYVGNNEDYQVQEQQGLTYNFEDKDPQNWLKSDFPARYIYEKILPEVVQRYSPSTFYHPGSPWGDGKI--------TSDPTVGDMH-------QWNVWHGTQEKYQIFDTLGGRFNSEFGMEAFPHMSTIEYFVENEKDKYPQSHVLDFHNKADGHERRIA--------TYLVENLRTATDLETYIYLTQVVQAETMMFGYRGWRRQWGDERHCG-----GALLWQLN 623          

BLAST of Beta-mannosidase vs. nr
Match: gi|1227973104|ref|XP_021917998.1| (beta-mannosidase [Zootermopsis nevadensis] >gi|1227973106|ref|XP_021917999.1| beta-mannosidase [Zootermopsis nevadensis] >gi|1227973108|ref|XP_021918000.1| beta-mannosidase [Zootermopsis nevadensis] >gi|646717625|gb|KDR20416.1| Beta-mannosidase [Zootermopsis nevadensis])

HSP 1 Score: 528.865 bits (1361), Expect = 2.601e-173
Identity = 285/665 (42.86%), Postives = 406/665 (61.05%), Query Frame = 0

Query:   59 LAGSDWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFVVEASIFEDKVKKWWPNGMGEQNLYQLRVFLVS-DGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVD-TLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPV-DHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNG-KIDR-GQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFETDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            L+G+ WKV+NANG+I   A VPGGIY+DL  AGIL   +Y+RFND+ Y W+  ENWTYS  F + + + +   + L   G+DTVA +  ND  +G T+NMFVRYSF +   I+    N L V+F+S +  A+ ++++ +  HY VPP  VP +Y GE HAN +RK Q+SF WD GP+FPS+G+++ + ++AF  A I  +T    L ++     W   + I +++ E +    G      E+   L     +  + L P++       +   I ++KV+ WWPNG G Q LY L V +   +  ++    + IGFR ++LI++P+ EL      NG TFY K+N+  +F KGSN +P H LPE+  + DV   LL  A+ +HMNM+RVWGGGLY+SD FYQ ADE+GI+IWQDFMFACS+YPT  EFL +V++EV  QV RL HHPS++     W   N N   + D+W  S +N S Y  DY+ LY  TI+  V  E  T  ++ SSP+NG + +R G +  +P S  +GD+H+Y    + WNP  YP  R ASEYGFQSFPS++TL + S  +DL   S+F++HRQH  G N+E+     ++ +  L  +    +     IY AQ+NQA S+   TE +RR  ++ T DG+G TMGALYWQLN
Sbjct:   29 LSGT-WKVRNANGNIEVPAVVPGGIYTDLRAAGILERDIYYRFNDIAYRWVSMENWTYSTNFTVEEELIEKQELFLVFHGVDTVASVYLNDEHIGNTDNMFVRYSFPVKSVIK-SGNNELEVRFESSITAAKERYQQQAL-HYPVPPTCVPDEYRGECHANHLRKMQASFSWDWGPAFPSAGLWKEVVLEAFDEAVIREVTTSATLSDN----TWLLNVGIFLEAGEQVLKVYG----KFEARLELDVDPVAKEAVLLPDEPGQFYTTLTLEIPKEKVELWWPNGFGSQKLYNLSVDVKDRNDTVIGQASLRIGFRTIELIEEPV-EL-----GNGLTFYIKVNNVPIFSKGSNWIPSHVLPEKSAEPDVIYDLLSSAKVAHMNMLRVWGGGLYESDLFYQLADEMGILIWQDFMFACSLYPTNNEFLQSVKKEVTQQVRRLHHHPSIII----WAGNNENEAALRDNWYGSGSNYSLYKSDYIKLYVDTIRPAVLAEDSTRPFIVSSPTNGIESEREGYIADNPYSSLYGDVHYYNYLADAWNPNNYPTTRFASEYGFQSFPSLNTLSKVSIPSDLSMQSQFIDHRQHHPGGNNELRIEILQNLQ--LQKYATPEEEFPTFIYFAQINQAMSIKTATEFYRRGRSLHTADGQGSTMGALYWQLN 670          

BLAST of Beta-mannosidase vs. nr
Match: gi|1339072353|ref|XP_023723402.1| (beta-mannosidase isoform X2 [Cryptotermes secundus] >gi|1339072355|ref|XP_023723403.1| beta-mannosidase isoform X2 [Cryptotermes secundus] >gi|1330879524|gb|PNF17252.1| Beta-mannosidase [Cryptotermes secundus])

HSP 1 Score: 526.554 bits (1355), Expect = 3.483e-172
Identity = 288/669 (43.05%), Postives = 404/669 (60.39%), Query Frame = 0

Query:   62 SDWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQS--AKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEGIEGDLGVEIHDIESG-EPLFGTTASVVSE---LFP-EKGALNEFVVEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGR-ILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVD-TLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPV-DHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNG-KIDR-GQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFETDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            S W V+NANGSI   A VPGGIY+DL  AG+L + +Y+RFND+NY W+ RENWTYS+ F + +   +   I L   G+DTVA +  ND  +G T+NMFVRYSF +   +Q   +N L V+F+S +  A  ++++ +   Y V P  VP +Y GE H N +RK Q+SF WD GP+FPS+G+++ + + AF S  A ++ +T    L+++     W  +I I +++        G +I  I    E +       V+E   L P E G  N   +   I +DKV+ WWPN +G Q LY L V +  +   I     + IGFR ++L+Q+P++         G TFY KIN+  +F KGSN +P H LPE+    D+  TLL  A+ +HMNM+RVWGGG+Y+SD FYQ ADELGI+IWQDFMFACS+YPT  EFL +V++EV  QV RL HHPSVV     W   N N   + D+W  S +    Y  DY+ LY  TI+ +V  E  T  ++ SSP+NG + +R G + ++P S  +GD+H+Y    + WNP  YP  R ASEYGFQSFPS+ +L + S  +DL   S F++HRQH  G N E+     +H +  L  ++   +     IY AQ+NQA S+   TE +RR  +++T DG+G+TMGALYWQLN
Sbjct:   45 SAWTVRNANGSIEVPAVVPGGIYTDLRAAGVLTQDIYYRFNDINYRWVSRENWTYSRNFIVEEEFIQMDQIFLVFHGVDTVASVYLNDKHIGNTDNMFVRYSFPVKSVVQ-RGENRLEVRFESSITAAEQQYQKQALT-YPVLPTCVPDEYRGECHVNHLRKMQASFSWDWGPAFPSAGLWKKVVLYAFVSDVAILQEVTASAKLIDN----TWLLQIGIFLEA--------GKDILQISGKFEAILELDVIPVTEEAVLLPNEPGQFNT-TLYMQIPKDKVELWWPNELGSQKLYNLSVRVKDNNDYIYGQMSLKIGFRTIELVQEPVES------GEGLTFYIKINNVPIFSKGSNWIPSHVLPEKSADPDIIYTLLSSAKAAHMNMLRVWGGGMYESDLFYQLADELGILIWQDFMFACSLYPTNSEFLQSVKKEVTQQVRRLHHHPSVVI----WAGNNENEAALRDNWYGSSSRFDLYKADYIKLYVDTIRPVVLAEDSTRPYIVSSPTNGIESEREGYIAENPYSSLYGDVHYYNYIADAWNPNNYPNTRFASEYGFQSFPSLKSLSEVSIPSDLIMQSAFIDHRQHHPGGNSELRLEILQHLQ--LRNYETPEEEFPTFIYFAQINQAMSIKTATEFYRRGRSLLTSDGQGVTMGALYWQLN 686          

BLAST of Beta-mannosidase vs. nr
Match: gi|1339072357|ref|XP_023723404.1| (beta-mannosidase isoform X3 [Cryptotermes secundus] >gi|1339072359|ref|XP_023723405.1| beta-mannosidase isoform X3 [Cryptotermes secundus] >gi|1330879525|gb|PNF17253.1| Beta-mannosidase [Cryptotermes secundus] >gi|1330879527|gb|PNF17255.1| Beta-mannosidase [Cryptotermes secundus] >gi|1330879528|gb|PNF17256.1| Beta-mannosidase [Cryptotermes secundus])

HSP 1 Score: 525.398 bits (1352), Expect = 5.220e-172
Identity = 288/669 (43.05%), Postives = 404/669 (60.39%), Query Frame = 0

Query:   62 SDWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQS--AKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEGIEGDLGVEIHDIESG-EPLFGTTASVVSE---LFP-EKGALNEFVVEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGR-ILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVD-TLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPV-DHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNG-KIDR-GQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFETDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            S W V+NANGSI   A VPGGIY+DL  AG+L + +Y+RFND+NY W+ RENWTYS+ F + +   +   I L   G+DTVA +  ND  +G T+NMFVRYSF +   +Q   +N L V+F+S +  A  ++++ +   Y V P  VP +Y GE H N +RK Q+SF WD GP+FPS+G+++ + + AF S  A ++ +T    L+++     W  +I I +++        G +I  I    E +       V+E   L P E G  N   +   I +DKV+ WWPN +G Q LY L V +  +   I     + IGFR ++L+Q+P++         G TFY KIN+  +F KGSN +P H LPE+    D+  TLL  A+ +HMNM+RVWGGG+Y+SD FYQ ADELGI+IWQDFMFACS+YPT  EFL +V++EV  QV RL HHPSVV     W   N N   + D+W  S +    Y  DY+ LY  TI+ +V  E  T  ++ SSP+NG + +R G + ++P S  +GD+H+Y    + WNP  YP  R ASEYGFQSFPS+ +L + S  +DL   S F++HRQH  G N E+     +H +  L  ++   +     IY AQ+NQA S+   TE +RR  +++T DG+G+TMGALYWQLN
Sbjct:   32 SAWTVRNANGSIEVPAVVPGGIYTDLRAAGVLTQDIYYRFNDINYRWVSRENWTYSRNFIVEEEFIQMDQIFLVFHGVDTVASVYLNDKHIGNTDNMFVRYSFPVKSVVQ-RGENRLEVRFESSITAAEQQYQKQALT-YPVLPTCVPDEYRGECHVNHLRKMQASFSWDWGPAFPSAGLWKKVVLYAFVSDVAILQEVTASAKLIDN----TWLLQIGIFLEA--------GKDILQISGKFEAILELDVIPVTEEAVLLPNEPGQFNT-TLYMQIPKDKVELWWPNELGSQKLYNLSVRVKDNNDYIYGQMSLKIGFRTIELVQEPVES------GEGLTFYIKINNVPIFSKGSNWIPSHVLPEKSADPDIIYTLLSSAKAAHMNMLRVWGGGMYESDLFYQLADELGILIWQDFMFACSLYPTNSEFLQSVKKEVTQQVRRLHHHPSVVI----WAGNNENEAALRDNWYGSSSRFDLYKADYIKLYVDTIRPVVLAEDSTRPYIVSSPTNGIESEREGYIAENPYSSLYGDVHYYNYIADAWNPNNYPNTRFASEYGFQSFPSLKSLSEVSIPSDLIMQSAFIDHRQHHPGGNSELRLEILQHLQ--LRNYETPEEEFPTFIYFAQINQAMSIKTATEFYRRGRSLLTSDGQGVTMGALYWQLN 673          

BLAST of Beta-mannosidase vs. nr
Match: gi|1339072351|ref|XP_023723400.1| (beta-mannosidase isoform X1 [Cryptotermes secundus] >gi|1330879526|gb|PNF17254.1| Beta-mannosidase [Cryptotermes secundus])

HSP 1 Score: 526.554 bits (1355), Expect = 9.129e-172
Identity = 288/669 (43.05%), Postives = 404/669 (60.39%), Query Frame = 0

Query:   62 SDWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQS--AKIEYLTFETDLVEDEGQRQWKSKIKIIIQSEEGIEGDLGVEIHDIESG-EPLFGTTASVVSE---LFP-EKGALNEFVVEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGR-ILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVD-TLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPV-DHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNG-KIDR-GQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFETDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            S W V+NANGSI   A VPGGIY+DL  AG+L + +Y+RFND+NY W+ RENWTYS+ F + +   +   I L   G+DTVA +  ND  +G T+NMFVRYSF +   +Q   +N L V+F+S +  A  ++++ +   Y V P  VP +Y GE H N +RK Q+SF WD GP+FPS+G+++ + + AF S  A ++ +T    L+++     W  +I I +++        G +I  I    E +       V+E   L P E G  N   +   I +DKV+ WWPN +G Q LY L V +  +   I     + IGFR ++L+Q+P++         G TFY KIN+  +F KGSN +P H LPE+    D+  TLL  A+ +HMNM+RVWGGG+Y+SD FYQ ADELGI+IWQDFMFACS+YPT  EFL +V++EV  QV RL HHPSVV     W   N N   + D+W  S +    Y  DY+ LY  TI+ +V  E  T  ++ SSP+NG + +R G + ++P S  +GD+H+Y    + WNP  YP  R ASEYGFQSFPS+ +L + S  +DL   S F++HRQH  G N E+     +H +  L  ++   +     IY AQ+NQA S+   TE +RR  +++T DG+G+TMGALYWQLN
Sbjct:   79 SAWTVRNANGSIEVPAVVPGGIYTDLRAAGVLTQDIYYRFNDINYRWVSRENWTYSRNFIVEEEFIQMDQIFLVFHGVDTVASVYLNDKHIGNTDNMFVRYSFPVKSVVQ-RGENRLEVRFESSITAAEQQYQKQALT-YPVLPTCVPDEYRGECHVNHLRKMQASFSWDWGPAFPSAGLWKKVVLYAFVSDVAILQEVTASAKLIDN----TWLLQIGIFLEA--------GKDILQISGKFEAILELDVIPVTEEAVLLPNEPGQFNT-TLYMQIPKDKVELWWPNELGSQKLYNLSVRVKDNNDYIYGQMSLKIGFRTIELVQEPVES------GEGLTFYIKINNVPIFSKGSNWIPSHVLPEKSADPDIIYTLLSSAKAAHMNMLRVWGGGMYESDLFYQLADELGILIWQDFMFACSLYPTNSEFLQSVKKEVTQQVRRLHHHPSVVI----WAGNNENEAALRDNWYGSSSRFDLYKADYIKLYVDTIRPVVLAEDSTRPYIVSSPTNGIESEREGYIAENPYSSLYGDVHYYNYIADAWNPNNYPNTRFASEYGFQSFPSLKSLSEVSIPSDLIMQSAFIDHRQHHPGGNSELRLEILQHLQ--LRNYETPEEEFPTFIYFAQINQAMSIKTATEFYRRGRSLLTSDGQGVTMGALYWQLN 720          

BLAST of Beta-mannosidase vs. nr
Match: gi|55728619|emb|CAH91050.1| (hypothetical protein [Pongo abelii])

HSP 1 Score: 486.493 bits (1251), Expect = 1.169e-158
Identity = 271/665 (40.75%), Postives = 401/665 (60.30%), Query Frame = 0

Query:   63 DWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIK--IIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFV-VEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNGK--IDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFE----TDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            +W + N NGS+    +VPG ++S L + G++ +S Y+RFND+NY W+  +NWTYSK F IP  + K+  + L L+G+DTV++I FN+  +G+T+NMF RYSFDI   ++ V  N++ ++FQS VLYA  + + H++  Y VPP+  P+   GE H NF+RK Q SF WD GPSFP+ GI++ + I+A+    + Y TF    + D+  ++W  +I+    + S + + G + V I       P   T  +   EL P K  +  FV +  +I    V+ WWP+G G Q  Y + +    DG +   K   + FR V+LI++P+K       S G +FYFKIN   +F+KGSN +P  +  +RV    +  LL+    ++MN +RVWGGG+Y+ D FY+  DELGIM+WQDFMFAC++YPT + FL +VR EV +Q+ RL+ HPSV+  S N  N N   + ++ +  S+ +   Y +DY+ LY K I  +V     +  ++TSSP+NG   +  G V Q+P S +FGD+HFY+     WN +++P  R ASEYG+QS+PS  TL++ S   D  F S+F  HRQH  G N +M+  A  HF+    TD +   +D+      IYL QV QA+ +  ETE +RRS + +  D +G TMGALYWQLN
Sbjct:   28 NWSICNGNGSLELPGAVPGCVHSALFQLGLIQDS-YYRFNDLNYRWVSLDNWTYSKEFKIPFEISKWQKVNLILEGVDTVSKILFNEVTIGETDNMFNRYSFDITNVVRDV--NSIELRFQSAVLYAAQQSKAHTR--YQVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNICHLNYFTFSP--LYDKSAQEWNLEIESAFDVVSSKPVGGQVIVAI-------PKLQTQQTYNIELQPGKRIVELFVNISKNI---TVETWWPHGHGNQTGYNMTILFELDGGLNIEKSAKVYFRTVELIEEPIKG------SPGLSFYFKINGFPIFLKGSNWIPADSFQDRVTSELLRLLLQSVVDANMNTLRVWGGGIYERDEFYELCDELGIMVWQDFMFACALYPTDQGFLDSVRAEVAYQIKRLKSHPSVIIWSGN--NENEEALMMNWYHISFTDRPIYIKDYVTLYVKNIGGLVLAGDKSRPFITSSPTNGAETVAEGWVSQNPNSNYFGDVHFYDYISGCWNWKVFPKARFASEYGYQSWPSFSTLEKVSSTEDWSFNSKFSLHRQHHEGGNKQMLYQAGLHFKLAQSTDPLRTFKDT------IYLTQVMQAQCVKTETEFYRRSRSEIV-DQQGHTMGALYWQLN 660          

BLAST of Beta-mannosidase vs. nr
Match: gi|1297676032|ref|XP_023075872.1| (beta-mannosidase isoform X1 [Piliocolobus tephrosceles])

HSP 1 Score: 489.574 bits (1259), Expect = 2.211e-158
Identity = 270/665 (40.60%), Postives = 403/665 (60.60%), Query Frame = 0

Query:   63 DWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKII--IQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFV-VEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNGK--IDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFE----TDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            +W + N NGS+    +VPG ++S L + G++ +S Y+RFND+NY WI  +NWTYSK F +P  + K+  + L L+G+DT+++I FN+  +G+T+NMF RYSFDI   ++ V  N++ ++FQS VLYA  + + H++  Y VPP+  P+   GE H NF+RK Q SF WD GPSFP+ GI++ + I+A+    + Y TF    + D+  ++W  +I+    + S + + G + V I       P   T      EL P K  +  F+ +  +I    V+ WWP+G G Q  Y + V    DG +   K   + FR V+LI++P+K       S G +FYFKIN   +F+KGSN +P  +  +RV    +  LL+    ++MN +RVWGGG+Y+ D FY+  DELGIM+WQDFMFAC++YPT + FL +VR EV +Q+ RL+ HPS++  S N  N N   + ++ +  SY +   Y +DY+ LY K I+++V     +  ++TSSP+NG   +  G V Q+P S +FGD+HFY+   + WN +++P  R ASEYG+QS+PS  TL++ S   D  F S+F  HRQH  G N +M+  A  HF+    TD +   +D+      IYL QV QA+ +  ETE +RRS + +  D +G TMGALYWQLN
Sbjct:   28 NWSICNGNGSLELPGAVPGCVHSALFQQGLIQDS-YYRFNDLNYRWISLDNWTYSKEFKLPFEISKWQKVNLILEGVDTISKILFNEVTIGETDNMFNRYSFDITNVVRDV--NSIELRFQSAVLYAAQQSKAHTR--YPVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNICHLNYFTFSP--IYDKSAQEWNLEIEAAFDVVSSKPVGGQVIVAI-------PKLQTQQMYNIELQPGKRIVELFLNISKNI---TVETWWPHGHGNQTGYNMTVLFELDGGLNIEKSAKVYFRTVELIEEPIKG------SPGLSFYFKINGFPIFLKGSNWIPADSFQDRVTSELLRLLLQSVVDANMNTLRVWGGGIYEQDEFYELCDELGIMVWQDFMFACALYPTDQGFLDSVRAEVAYQIKRLKSHPSIIIWSGN--NENEEALMMNWYHISYTDRPVYIKDYVTLYVKNIRELVLAGDKSRPFITSSPTNGAETVAEGWVSQNPNSNYFGDVHFYDYISDCWNWKVFPKARFASEYGYQSWPSFSTLEKVSSTEDWSFSSKFSLHRQHHEGGNKQMLYQAGLHFKLPKSTDPLRTFKDT------IYLTQVMQAQCVKTETEFYRRSRSEIV-DQQGHTMGALYWQLN 660          

BLAST of Beta-mannosidase vs. nr
Match: gi|1331936495|gb|PNJ84381.1| (MANBA isoform 1 [Pongo abelii])

HSP 1 Score: 488.804 bits (1257), Expect = 4.390e-158
Identity = 270/665 (40.60%), Postives = 404/665 (60.75%), Query Frame = 0

Query:   63 DWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIK--IIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFV-VEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNGK--IDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFE----TDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            +W + N NGS+    +VPG ++S L + G++ +S Y+RFND+NY W+  +NWTYSK F IP  + K+  + L L+G+DTV++I FN+  +G+T+NMF RYSFDI   ++ V  N++ ++FQS VLYA  + + H++  Y VPP+  P+   GE H NF+RK Q SF WD GPSFP+ GI++ + I+A+    + Y TF    + D+  ++W  +I+    + S + + G + V I       P   T  +   EL P K  +  FV +  +I    V+ WWP+G G Q  Y + +    DG +   K   + FR V+L+++P+K       S G +FYFKIN   +F+KGSN +P  +  +RV    +  LL+    ++MN +RVWGGG+Y+ D FY+  DELGIM+WQDFMFAC++YPT + FL +VR EV +Q+ RL+ HPSV+  S N  N N   + ++ +  S+ +   Y +DY+ LY K I+++V     +  ++TSSP+NG   +  G V Q+P S +FGD+HFY+   + WN +++P  R ASEYG+QS+PS  TL++ S   D  F S+F  HRQH  G N +M+  A  HF+    TD +   +D+      IYL QV QA+ +  ETE +RRS + +  D +G TMGALYWQLN
Sbjct:   28 NWSICNGNGSLELPGAVPGCVHSALFQQGLIQDS-YYRFNDLNYRWVSLDNWTYSKEFKIPFEISKWQKVNLILEGVDTVSKILFNEVTIGETDNMFNRYSFDITNVVRDV--NSIELRFQSAVLYAAQQSKAHTR--YQVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNICHLNYFTFSP--LYDKSAQEWNLEIESAFDVVSSKPVGGQVIVAI-------PKLQTQQTYNIELQPGKRIVELFVNISKNI---TVETWWPHGHGNQTGYNMTILFELDGGLNIEKSAKVYFRTVELVEEPIKG------SPGLSFYFKINGFPIFLKGSNWIPADSFQDRVTSELLRLLLQSVVDANMNTLRVWGGGIYERDEFYELCDELGIMVWQDFMFACALYPTDQGFLDSVRAEVAYQIKRLKSHPSVIIWSGN--NENEEALMMNWYHISFTDRPIYIKDYVTLYVKNIRELVLAGDKSRPFITSSPTNGAETVAEGWVSQNPNSNYFGDVHFYDYISDCWNWKVFPKARFASEYGYQSWPSFSTLEKVSSTEDWSFNSKFSLHRQHHEGGNKQMLYQAGLHFKLPQSTDPLRTFKDT------IYLTQVMQAQCVKTETEFYRRSRSEIV-DQQGHTMGALYWQLN 660          

BLAST of Beta-mannosidase vs. nr
Match: gi|197097914|ref|NP_001125103.1| (beta-mannosidase precursor [Pongo abelii] >gi|55726966|emb|CAH90241.1| hypothetical protein [Pongo abelii])

HSP 1 Score: 488.804 bits (1257), Expect = 4.437e-158
Identity = 270/665 (40.60%), Postives = 404/665 (60.75%), Query Frame = 0

Query:   63 DWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIK--IIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFV-VEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNGK--IDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFE----TDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            +W + N NGS+    +VPG ++S L + G++ +S Y+RFND+NY W+  +NWTYSK F IP  + K+  + L L+G+DTV++I FN+  +G+T+NMF RYSFDI   ++ V  N++ ++FQS VLYA  + + H++  Y VPP+  P+   GE H NF+RK Q SF WD GPSFP+ GI++ + I+A+    + Y TF    + D+  ++W  +I+    + S + + G + V I       P   T  +   EL P K  +  FV +  +I    V+ WWP+G G Q  Y + +    DG +   K   + FR V+L+++P+K       S G +FYFKIN   +F+KGSN +P  +  +RV    +  LL+    ++MN +RVWGGG+Y+ D FY+  DELGIM+WQDFMFAC++YPT + FL +VR EV +Q+ RL+ HPSV+  S N  N N   + ++ +  S+ +   Y +DY+ LY K I+++V     +  ++TSSP+NG   +  G V Q+P S +FGD+HFY+   + WN +++P  R ASEYG+QS+PS  TL++ S   D  F S+F  HRQH  G N +M+  A  HF+    TD +   +D+      IYL QV QA+ +  ETE +RRS + +  D +G TMGALYWQLN
Sbjct:   28 NWSICNGNGSLELPGAVPGCVHSALFQQGLIQDS-YYRFNDLNYRWVSLDNWTYSKEFKIPFEISKWQKVNLILEGVDTVSKILFNEVTIGETDNMFNRYSFDITNVVRDV--NSIELRFQSAVLYAAQQSKAHTR--YQVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNICHLNYFTFSP--LYDKSAQEWNLEIESAFDVVSSKPVGGQVIVAI-------PKLQTQQTYNIELQPGKRIVELFVNISKNI---TVETWWPHGHGNQTGYNMTILFELDGGLNIEKSAKVYFRTVELVEEPIKG------SPGLSFYFKINGFPIFLKGSNWIPADSFQDRVTSELLRLLLQSVVDANMNTLRVWGGGIYERDEFYELCDELGIMVWQDFMFACALYPTDQGFLDSVRAEVAYQIKRLKSHPSVIIWSGN--NENEEALMMNWYHISFTDRPIYIKDYVTLYVKNIRELVLAGDKSRPFITSSPTNGAETVAEGWVSQNPNSNYFGDVHFYDYISDCWNWKVFPKARFASEYGYQSWPSFSTLEKVSSTEDWSFNSKFSLHRQHHEGGNKQMLYQAGLHFKLPQSTDPLRTFKDT------IYLTQVMQAQCVKTETEFYRRSRSEIV-DQQGHTMGALYWQLN 660          

BLAST of Beta-mannosidase vs. nr
Match: gi|81878533|sp|Q8K2I4.1|MANBA_MOUSE (RecName: Full=Beta-mannosidase; AltName: Full=Lysosomal beta A mannosidase; AltName: Full=Mannanase; Short=Mannase; Flags: Precursor >gi|21595011|gb|AAH31409.1| Mannosidase, beta A, lysosomal [Mus musculus])

HSP 1 Score: 488.804 bits (1257), Expect = 4.992e-158
Identity = 280/673 (41.60%), Postives = 399/673 (59.29%), Query Frame = 0

Query:   55 PKHDLAGSDWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIK--IIIQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFVVEASIFED-KVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNG--KIDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFE----TDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            P + L+GS W+V N NGS+   A+VPG ++S L + G++ +  Y+RFND+NY WI  +NWTYS  F IP ++ ++  + L  DG+DTVAEI FN+  +GKT+NMF  YSFDI   ++ V  N+L ++F+S V YA  + + H+   Y VPPE  P++  GE H NFIRK Q SF WD GPSFPS GI++ + I+A+  A ++YLTF    V D   + W  +IK    + S + + G + V I       P   T  +   EL  E+  +   V    I +D  V+ WWP G G Q  Y + +    DG +   K   + FR VQLI++ +K       S G +FYFKIN   +F+KGSN +P  +  ++V    +  L +    ++MN +RVWGGG+Y+ D FY   DELGIM+WQDFMFA ++YPT   FL +VR+EV +QV RL+ HPS++  S N  N N   + V+ +  +  +   Y +DY+ LY K I+KIV  E  +  ++ SSP+NG   ++ G +  DP S  +GDIHFY  A + WN +I+P  RL SEYG+QS+PS  TL++ S + D  + SRF  HRQH    N +M+     HF+    TD +   +D+      IYL QV QA+ +  ETE + RS + +  DG+G TMGALYWQLN
Sbjct:   21 PSYSLSGS-WRVSNGNGSLELPATVPGYVHSALHQHGLIQDP-YYRFNDLNYRWISLDNWTYSTEFKIPFNLSEWQKVKLIFDGVDTVAEILFNNVTIGKTDNMFTGYSFDITNVVKDV--NSLKLQFRSAVQYAECQSKAHTS--YRVPPECPPVEQKGECHVNFIRKAQCSFSWDWGPSFPSQGIWKDVRIEAYNIAHLDYLTFLP--VYDNASQAWNIEIKASFDVASSKSVGGQVTVAI-------PQLKTQQTNDIELQQEQRIVKLLV---KIRKDVAVETWWPRGHGNQTGYNMTILFALDGGLKIEKAAKVYFRTVQLIEEGIKG------SPGLSFYFKINGLPIFLKGSNWIPADSFQDKVTSDRLQLLFQSVVDANMNTLRVWGGGIYEQDEFYALCDELGIMVWQDFMFASALYPTEPGFLASVRKEVTYQVRRLKSHPSIIIWSGN--NENEVALSVNWFHVNPRDMKTYIDDYVTLYVKNIRKIVLSEDKSRPFIASSPTNGMKTMEEGWISYDPYSIQYGDIHFYNYADDCWNWKIFPKARLVSEYGYQSWPSFSTLEKVSSQEDWAYNSRFSLHRQHHEDGNHQMLHQVKMHFKLPQGTDPLRTFKDT------IYLTQVMQAQCIKTETEFYLRSRSEIV-DGKGHTMGALYWQLN 660          

BLAST of Beta-mannosidase vs. nr
Match: gi|114595438|ref|XP_001169005.1| (beta-mannosidase isoform X1 [Pan troglodytes] >gi|1331797096|gb|PNI56677.1| MANBA isoform 2 [Pan troglodytes])

HSP 1 Score: 488.419 bits (1256), Expect = 7.747e-158
Identity = 270/665 (40.60%), Postives = 403/665 (60.60%), Query Frame = 0

Query:   63 DWKVQNANGSISCQASVPGGIYSDLERAGILNESVYFRFNDVNYAWIGRENWTYSKVFAIPKSMKKFSNIMLNLDGIDTVAEITFNDHQVGKTENMFVRYSFDILEHIQWVKKNTLVVKFQSPVLYARSKFEEHSKNHYIVPPESVPMDYNGENHANFIRKTQSSFGWDIGPSFPSSGIYRPIWIQAFQSAKIEYLTFETDLVEDEGQRQWKSKIKII--IQSEEGIEGDLGVEIHDIESGEPLFGTTASVVSELFPEKGALNEFV-VEASIFEDKVKKWWPNGMGEQNLYQLRVFLVSDGRILSTKVISIGFRIVQLIQQPLKELYPSLPSNGTTFYFKINHRAVFVKGSNLVPIHALPERVLKRDVDTLLEFAQKSHMNMIRVWGGGLYQSDYFYQRADELGIMIWQDFMFACSMYPTTKEFLGNVRQEVLHQVWRLQHHPSVVALSKNWYNTNHNGVPVDHWKNSYANTSRYYEDYLWLYEKTIKKIVKKEAPTTVWVTSSPSNGK--IDRGQVGQDPGSPFFGDIHFYERAQNQWNPQIYPIPRLASEYGFQSFPSIHTLKQYSQEADLYFPSRFLNHRQHGHGSNDEMVSTASEHFE----TDLIVHQRDSDGLAKLIYLAQVNQAESLWVETETFRRSMNIVTKDGRGMTMGALYWQLN 718
            +W + N NGS+    +VPG ++S L + G++ +S Y+RFND+NY W+  +NWTYSK F IP  + K+  + L L+G+DTV++I FN+  +G+T+NMF RYSFDI   ++ V  N++ ++FQS VLYA  + + H++  Y VPP+  P+   GE H NF+RK Q SF WD GPSFP+ GI++ + I+A+    + Y TF    + D+  ++W  +I+    + S + + G + V I       P   T  +   EL P K  +  FV +  +I    V+ WWP+G G Q  Y + V    DG +   K   + FR V+LI++P+K       S G +FYFKIN   +F+KGSN +P  +  +RV    +  LL+    ++MN +RVWGGG+Y+ D FY+  DELGIM+WQDFMFAC++YPT + FL +V  EV +Q+ RL+ HPS++  S N  N N   + ++ +  S+ +   Y +DY+ LY K I+++V     +  ++TSSP+NG   +  G V Q+P S +FGD+HFY+   + WN +++P  R ASEYG+QS+PS  TL++ S   D  F S+F  HRQH  G N +M+  A  HF+    TD +   +D+      IYL QV QA+ +  ETE +RRS + +  D +G TMGALYWQLN
Sbjct:   28 NWSICNGNGSLELPGAVPGCVHSALFQQGLIQDS-YYRFNDLNYRWVSLDNWTYSKEFKIPFEISKWQKVNLILEGVDTVSKILFNEVTIGETDNMFNRYSFDITNVVRDV--NSIELRFQSAVLYAAQQSKAHTR--YQVPPDCPPLVQKGECHVNFVRKEQCSFSWDWGPSFPTQGIWKDVRIEAYNICHLNYFTFSP--IYDKSAQEWNLEIESTFDVVSSKPVGGQVIVAI-------PKLQTQQTYSIELQPGKRIVELFVNISKNI---TVETWWPHGHGNQTGYNMTVLFELDGGLNIEKSAKVYFRTVELIEEPIKG------SPGLSFYFKINGFPIFLKGSNWIPADSFQDRVTSELLRLLLQSVVDANMNTLRVWGGGIYEQDEFYELCDELGIMVWQDFMFACALYPTDQGFLDSVTAEVAYQIKRLKSHPSIIIWSGN--NENEEALMMNWYHISFTDRPIYIKDYVTLYVKNIRELVLAGDKSRPFITSSPTNGAETVAEGWVSQNPNSNYFGDVHFYDYISDCWNWKVFPKARFASEYGYQSWPSFSTLEKVSSTEDWSFNSKFSLHRQHHEGGNKQMLYQAGLHFKLPQSTDPLRTFKDT------IYLTQVMQAQCVKTETEFYRRSSSEIV-DQQGHTMGALYWQLN 660