BLAST of ubiquitin-activating enzyme e1 vs. L. salmonis genes
Match: EMLSAG00000009394 (supercontig:LSalAtl2s:LSalAtl2s5:281454:284794:-1 gene:EMLSAG00000009394 transcript:EMLSAT00000009394 description:"maker-LSalAtl2s5-snap-gene-2.20")

HSP 1 Score: 1202.19 bits (3109), Expect = 0.000e+0
Identity = 604/1018 (59.33%), Postives = 748/1018 (73.48%), Query Frame = 0

Query:  130 DIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTA--SSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAVDEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSI--GDVSKFA-KYTKGGIAIQVKMPTQVAFKSLQEALSD-DGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGAR-NGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPD-FIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQE-TTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKLLKK 1138
            +IDE LYSRQLYVLGH+AM+KMA S+VL+ G  GLGVE+ KNVILGG KSV+L+D  ++   + S  F  T A +G  R +     L ELN YV   A  S E L       FSV+V+T ++L++Q+ +++  R  GI L+VADTRG ++Q+F DFGP F V D+NGE  +S MIS++ E G VTCQDETRHGFEDGD VTF+EV+G+  +N    + IKV GPYTF++    +  F+  Y  GGIA QVK P +V+FK+L+EA+ + + L ++TDFGK D P  +H  F T+HE+ + + RLPKPW   DA  FV +A+ L     K   DD  E F+ QF+K+ +G+L PMA A+GG VA E MKACSGKFMP++Q++YFDALECLP DTS LT   C P  +RYDGQ+AVFG+ FQ+K+  QKYFVVGAGAIGCELLKNFAM+GLG    G+I VTDMD IE+SNLNRQFLFRPWDVQK KS  A AA KAMNPD+N+V+ ENRVG DT+ VYNDDFFE LDGVANALDN+EAR Y+DRRCVYYRLPLLESGTLG+KGNTQVV P++TESYSSSQDPPEK+IP+CTL +FPN+IEHTLQWARDLFEGT++QGPL+ KQ+L EPD FI  TM+L G QP +T +++KK+LVDE+P  F DCV WARL +Q+L+ NQI QLLH+FP D+  ++G  FWSGPKRCPK L F+T   +H+ F+++AANL A+VY I    D   +   ++   V  F P+  V I  ND +AQ  +  +  +D + L K+   +P K     L V  L+FEKDDD NFHMDFIVA SNLRA+NY I  AD+  SKLIAGRIIPAIATTTSLVAGL+SLELYKLVQGH  VE FKNGF NLALPF+TFS+PI+AP+ KYY+ EWTLWDRFV+DN +G+EMTL QF+ +FK K  LEI+M++QGVSMLYSFFM    REER+KM MSKVV  VSKK                   GEDV++P+VQY L KK
Sbjct:   41 EIDEXLYSRQLYVLGHDAMRKMASSDVLLVGCGGLGVEIVKNVILGGXKSVSLYDPASIGKSEQSCNFSATDAYVGTRRDKACLSGLRELNAYVNVVALESIEDL-----SPFSVIVVTEATLSQQMELAKKARSLGIKLIVADTRGXFSQIFCDFGPQFMVSDSNGEAALSVMISSIAEDGTVTCQDETRHGFEDGDTVTFSEVQGLEGINDFGNVQIKVTGPYTFTVPTDTLKTFSGNYKSGGIAXQVKXPVKVSFKTLEEAMENPEELMLLTDFGKMDSPIRIHNCFKTMHEFVRVNNRLPKPWHGEDADKFVKIAQSL---LPKFNADDDEEYFR-QFSKVCSGNLCPMAXALGGIVAXEXMKACSGKFMPIRQYLYFDALECLPEDTSNLTYENCAPQGNRYDGQVAVFGKEFQEKMLAQKYFVVGAGAIGCELLKNFAMVGLGCSPEGKIFVTDMDHIERSNLNRQFLFRPWDVQKPKSLVAXAAXKAMNPDVNIVAHENRVGQDTDHVYNDDFFENLDGVANALDNVEARVYIDRRCVYYRLPLLESGTLGSKGNTQVVXPDLTESYSSSQDPPEKTIPMCTLTHFPNKIEHTLQWARDLFEGTFAQGPLNGKQFLSEPDSFISSTMKLPGVQPFKTFEMLKKLLVDERPRSFKDCVLWARLLWQDLFFNQIAQLLHSFPPDRVNNSGLPFWSGPKRCPKPLKFETEDDLHISFLLNAANLYADVYSIPQCRDASAVRAAVEGFEVPIFVPKSNVEIPENDTQAQELANSKVVSDMDDLGKLVDSIPKK--IDSLTVRSLDFEKDDDTNFHMDFIVAASNLRADNYSIGRADKHISKLIAGRIIPAIATTTSLVAGLISLELYKLVQGHKKVEQFKNGFVNLALPFVTFSEPIRAPISKYYDNEWTLWDRFVIDNSDGEEMTLKQFVEFFKNKHGLEISMITQGVSMLYSFFMSGTSREERLKMPMSKVVSVVSKKX------------------GEDVEIPFVQYNLPKK 1029          

BLAST of ubiquitin-activating enzyme e1 vs. L. salmonis genes
Match: EMLSAG00000005615 (supercontig:LSalAtl2s:LSalAtl2s3012:1690:6443:1 gene:EMLSAG00000005615 transcript:EMLSAT00000005615 description:"augustus_masked-LSalAtl2s3012-processed-gene-0.1")

HSP 1 Score: 142.895 bits (359), Expect = 4.883e-36
Identity = 76/202 (37.62%), Postives = 112/202 (55.45%), Query Frame = 0

Query:  535 AVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQ 736
             +F  S  +K+   K  VVGAG IGCELLKN  ++G       I V D+D I+ +NLNRQFLF+   V K+KS+ A  +    NPD+N+ S  + +       Y   F++    V NALDN  AR +++R C+   +PL+ESGT G  G   V+    TE +     P +K+ P CT++N P++  H + WA+ LF   + +
Sbjct:    7 GIFEDSLAEKIKSSKILVVGAGGIGCELLKNIVLVGFA----NIEVIDLDTIDVTNLNRQFLFQKRHVGKSKSTVARESALKFNPDVNIKSYHDSI---LNPDYGVSFYKKFALVLNALDNNTARNHVNRMCLASGIPLVESGTAGYLGQVSVIKKGDTECFECQPKPRQKTFPGCTIRNTPSEPIHCIVWAKHLFNQLFGE 201          

HSP 2 Score: 63.5438 bits (153), Expect = 1.839e-10
Identity = 35/136 (25.74%), Postives = 72/136 (52.94%), Query Frame = 0

Query:  149 KKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVE-----AFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQV 279
            +K+  S +LV G  G+G E+ KN++L G  ++ + D DT+ + +L+ QF F    +G++++ VA     + N  V   +  + ++      +F ++F++V+    +   +  ++ +   +GI LV + T G   QV
Sbjct:   15 EKIKSSKILVVGAGGIGCELLKNIVLVGFANIEVIDLDTIDVTNLNRQFLFQKRHVGKSKSTVARESALKFNPDVNIKSYHDSILNPDYGVSFYKKFALVLNALDNNTARNHVNRMCLASGIPLVESGTAGYLGQV 150          

HSP 3 Score: 58.9214 bits (141), Expect = 5.628e-9
Identity = 28/68 (41.18%), Postives = 43/68 (63.24%), Query Frame = 0

Query:  927 LEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQ 994
            L ++KDDDA   MDF+ A +N+R+  + I    R   K +AG IIPAI TT +++AG + +E  K++ 
Sbjct:  334 LVWDKDDDA--AMDFVTACANIRSHIFSIQQKSRFDVKXMAGNIIPAIXTTNAVIAGCIVMEAIKILN 399          

BLAST of ubiquitin-activating enzyme e1 vs. L. salmonis genes
Match: EMLSAG00000006362 (supercontig:LSalAtl2s:LSalAtl2s3489:537:2379:1 gene:EMLSAG00000006362 transcript:EMLSAT00000006362 description:"maker-LSalAtl2s3489-augustus-gene-0.7")

HSP 1 Score: 110.538 bits (275), Expect = 1.131e-25
Identity = 69/197 (35.03%), Postives = 102/197 (51.78%), Query Frame = 0

Query:  549 KYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYR-------------LPLLESGTLGTKGNTQVVIPNVTESYSSSQD--PPEKSIPICTLKNFPNQIEHTLQWARDLF 730
            +  V+GAG +GCELLKN A++G  + N    V DMD I+ SNLNRQFLFR  DV K K+  AA  I        V+     +    ES Y   F  +L G    LD+I AR +++   V                +P+++ GT G KGN +V++P +T     + D  PP+ + P+CT+ + P   EH +++ + L 
Sbjct:   50 RLLVIGAGGLGCELLKNVALMGFKSIN----VIDMDTIDVSNLNRQFLFRATDVGKFKAEAAAEFINKRIKGAQVIPHNKTIQEFDESFYR-QFHIILCG----LDSIVARRWINGMVVSLLEYDDDGSVIQSSVIPIIDGGTEGFKGNARVILPGLTACIECTLDLYPPQLNFPLCTIAHTPRLPEHCIEYVKVLL 237          

HSP 2 Score: 57.3806 bits (137), Expect = 1.226e-8
Identity = 36/116 (31.03%), Postives = 61/116 (52.59%), Query Frame = 0

Query:  146 EAMKKMAQS-NVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAH---GKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRIS 257
            E +K +  S  +LV G  GLG E+ KNV L G KS+ + D DT+ + +L+ QF F   D+G+ +A+ A     K  +    +    + +   E+F RQF +++    S+  +  I+
Sbjct:   40 EILKFLQSSCRLLVIGAGGLGCELLKNVALMGFKSINVIDMDTIDVSNLNRQFLFRATDVGKFKAEAAAEFINKRIKGAQVIPHNKTIQEFDESFYRQFHIILCGLDSIVARRWIN 155          

BLAST of ubiquitin-activating enzyme e1 vs. L. salmonis genes
Match: EMLSAG00000004855 (supercontig:LSalAtl2s:LSalAtl2s256:394129:396304:1 gene:EMLSAG00000004855 transcript:EMLSAT00000004855 description:"maker-LSalAtl2s256-augustus-gene-4.12")

HSP 1 Score: 102.449 bits (254), Expect = 1.310e-23
Identity = 56/164 (34.15%), Postives = 88/164 (53.66%), Query Frame = 0

Query:  126 AGGQDIDES---LYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASS---EPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDF 283
            +G  +I E+   LY RQ+ + G +A K++  + VLV G+ GLG EV+KN++L GVKS+ + D + V  +D ++QF      +G NRA+ +  +L +LN  V   A S   E   + + R F +V  T   L+E +RI+E  R   +     D  G +   F D 
Sbjct:    2 SGSMEITEAEAQLYDRQIRLWGLDAQKRLRNARVLVIGMSGLGTEVSKNIVLAGVKSLIMIDPENVCAKDAASQFLAPRDKMGFNRAEASRERLQQLNSMVEVRAESGKVEDKSDDYFRDFDIVCATGLVLSEYMRINEACRARNVKFFCGDVTGFFGYCFADL 165          

BLAST of ubiquitin-activating enzyme e1 vs. L. salmonis genes
Match: EMLSAG00000012312 (supercontig:LSalAtl2s:LSalAtl2s899:315986:320094:1 gene:EMLSAG00000012312 transcript:EMLSAT00000012312 description:"maker-LSalAtl2s899-augustus-gene-3.13")

HSP 1 Score: 62.7734 bits (151), Expect = 3.942e-10
Identity = 46/165 (27.88%), Postives = 74/165 (44.85%), Query Frame = 0

Query:  121 PPAHPAGGQDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYV---TTTASSEPLVEA---FIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQV 279
            PP  PA  +      Y RQL + G      + +S+V +      G E+ K+++L GV + T+ D   V+  DL   F+   +D+ QNR +VA   L ELN  V         E ++ A   F   F++V+   +       IS++    GI L+   T G  + +
Sbjct:   11 PPCSPAQSEKARR--YDRQLRLWGDHGQILLEKSHVCLLHANATGTEILKSLVLPGVGAFTIVDDKKVSGEDLGNNFFLEESDLDQNRGEVAARLLLELNPEVRGDCVXDCPEQILRAEPNFFLGFNIVIACEAREDTLKTISKMLWIEGIPLITVRTYGFISYI 173          

BLAST of ubiquitin-activating enzyme e1 vs. L. salmonis genes
Match: EMLSAG00000004149 (supercontig:LSalAtl2s:LSalAtl2s219:326510:328743:-1 gene:EMLSAG00000004149 transcript:EMLSAT00000004149 description:"maker-LSalAtl2s219-snap-gene-3.38")

HSP 1 Score: 54.299 bits (129), Expect = 1.203e-7
Identity = 50/194 (25.77%), Postives = 87/194 (44.85%), Query Frame = 0

Query:  529 RYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDC-IEKSNLNRQFLFRPWDVQKA---KSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVL---DGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVI--PNVTESYSS--SQDPPEKSIPICT 711
            RY  Q+ +F    + +L  +  ++VGAG                   G + + D D  IE SNL+RQ L  P D  K+   K+ +AA ++  +NP++       ++   T ++ + +  E++   D V +A DN+  R  ++  CV    PL+    L  +G   V    PNV  +Y       PP +++  C+
Sbjct:   37 RYSRQLIIFDNGLEDQLAIKSQYLVGAGV------------------GTLGILDFDLEIEYSNLHRQVL-HPHDRAKSLTSKADSAAISLGRLNPNI-------QIKPITTALTSQNALEIISEYDIVLDASDNVATRYLLNDACVLSEKPLVSGSALRFEGQLTVYNYPPNVGTTYRCLFPDPPPPETVTNCS 204          

BLAST of ubiquitin-activating enzyme e1 vs. SwissProt
Match: gi|6136092|sp|Q29504.1|UBA1_RABIT (RecName: Full=Ubiquitin-like modifier-activating enzyme 1; AltName: Full=Ubiquitin-activating enzyme E1)

HSP 1 Score: 1287.71 bits (3331), Expect = 0.000e+0
Identity = 636/1076 (59.11%), Postives = 774/1076 (71.93%), Query Frame = 0

Query:   66 NSPPAAKKLRLEEGAEGGPDASSPPRHKNGSANGFKPMTPDTQNGAGAPANGHNPPPAHPAGGQDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTF-EQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGA-RNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLD--NKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            +S P +KK R+      GPD    P      A    P  P       AP NG     A      DIDE LYSRQLYVLGHEAMK++  S+VLVSGL+GLGVE+AKN+ILGGVK+VTLHD  T    DLS+QFY    DIG+NRA+V+  +LAELN YV  TA + PLVE F+  F VVVLT S L +QLR+ E     GI LVVADTRGL+ Q+F DFG    + D+NGEQP+STM+S V  D  G+VTC DE RHGFE GD V+F+EV+GM ELN + P+ IKVLGPYTFSI D S F+ Y +GGI  QVK+P +++FKSL  +L++   F++TDF KF RPA +H+ F  LH++  +H R P+P +  DA + V +A+ +N +       D+  E      A +AAGDL P+ A +GG  AQEVMKACSGKFMP+ QW+YFDALECLP D   LTE +C P  +RYDGQ+AVFG   Q+KLG QKYF+VGAGAIGCELLKNFAMIGLG   NG+IIVTDMD IEKSNLNRQFLFRPWDV K KS TAAAA+  MNP + V S +NRVG DTE +Y+DDFF+ LDGVANALDN++AR YMDRRCVYYR PLLESGTLGTKGN QVVIP +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +  QYL +P F+E+T+RL G QP+E L+ V++ LV + P  +ADCV WA   +   Y N IRQLLHNFP DQ TS+G  FWSGPKRCP  L FD S  +H+D+V++AANL A+ YG+ GS D   + T+L+ V V  F P+ GV+I  +D E   QS   + D   L ++   LP      G K+  ++FEKDDD+NFHMDFIVA SNLRAENY I PADR KSKLIAG+IIPAIATTT+ V GLV LELYK+VQGH  ++ +KNGF NLALPF  FS+P+ AP  +YY +EWTLWDRF +     NG+EMTL QF++YFK + +LEITMLSQGVSMLYSFFMP  K +ER+   M+++V  VSK+ +  +V+ALV ELCCND+ GEDV+VPYV+Y +
Sbjct:    2 SSSPLSKKRRV-----SGPDPK--PGSNCSPAQSVLPQVP------SAPTNGM----AKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNSPLEDQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSTMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMTELNGNQPIEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLSASLAEPD-FVMTDFAKFSRPAQLHIGFQALHKFCAQHSRPPRPRNEEDAAELVTLARAVNSKASSAVQQDSLDEDLIRNLAFVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKESLTEDKCLPRQNRYDGQVAVFGSDLQEKLGRQKYFLVGAGAIGCELLKNFAMIGLGCGENGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVHQMNPHIRVTSHQNRVGPDTERIYDDDFFQTLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQLPQSWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVSNPLHLDYVMAAANLFAQTYGLAGSQDRAAVATLLQSVQVPEFTPKSGVKIHVSDQE--LQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRHLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTI 1057          

BLAST of ubiquitin-activating enzyme e1 vs. SwissProt
Match: gi|182702190|sp|A3KMV5.1|UBA1_BOVIN (RecName: Full=Ubiquitin-like modifier-activating enzyme 1; AltName: Full=Ubiquitin-activating enzyme E1)

HSP 1 Score: 1281.93 bits (3316), Expect = 0.000e+0
Identity = 625/1056 (59.19%), Postives = 766/1056 (72.54%), Query Frame = 0

Query:  102 PMTPDTQNGAGAPANGHNPPPAH---------PAGGQ-------DIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTF-EQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLG-ARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLD--NKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            P++   +     P  G N  PAH         PA G        DIDE LYSRQLYVLGHEAMK++  S+VLVSGL+GLGVE+AKN+ILGGVK+VTLHD  T    DLS+QFY    DIG+NRA+V+  +LAELN YV  +A + PLVE F+  F VVVLT S L +QLR+ E     GI LVVADTRGL+ Q+F DFG    + D+NGEQP+S M+S V  D  G+VTC DE RHGFE GD V+F+EV+GM ELN S PM IKVLGPYTFSI D S F+ Y +GGI  QVK+P +++FKSL  +L++   F++TDF K+ RPA +H+ F  LH +  +H R P+P +  DA + V +A+ +N + +      +  E    + A +AAGDL P+ A +GG  AQEVMKACSGKFMP+ QW+YFDALECLP D   LTE +C P  +RYDGQ+AVFG   Q++LG QKYF+VGAGAIGCELLKNFAMIGLG A +G+I+VTDMD IEKSNLNRQFLFRPWDV K KS TAAAA++ MNP + V S +NRVG DTE +Y+DDFF+ LDGV NALDN++AR YMDRRCVYYR PLLESGTLGTKGN QVVIP +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +  QYL +P F+E+T+RL G QP+E L+ V++ LV ++P  +ADCV WA   +   Y N IRQLLHNFP DQ TS+G  FWSGPKRCP  L FD S  +H+D+VI+AANL A+ YG+ GS D   + T+L+ V V  F P+ GV+I  +D E   QS   + D   L ++   LP      G K+  ++FEKDDD NFHMDFIVA SNLRAENY I PADR KSKLIAG+IIPAIATTT+ V GLV LELYK+VQGH  +  +KNGF NLALPF  FS+P+ AP  +YY +EWTLWDRF +     NG+EMTL QF++YFK + +LEITMLSQGVSMLYSFFMP  K +ER+   M+++V  VSK+ +  +V+ALV ELCCND+ GEDV+VPYV+Y +
Sbjct:    5 PLSKKRRVSGPDPKPGSNCSPAHSVLSEVPSVPANGMAKNVSDADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVSAYTGPLVEDFLSDFQVVVLTNSPLEDQLRVGEFCHSHGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMIELNGSQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPD-FVMTDFAKYSRPAQLHIGFQALHHFCAQHGRSPRPHNEEDAAELVTIAQAVNARSLPAVQQGSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQERLGKQKYFLVGAGAIGCELLKNFAMIGLGCAEDGEIVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVTNALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVSNPLHLDYVIAAANLFAQTYGLTGSQDRAAVATLLQSVQVPEFTPKSGVKIHVSDQE--LQSANASVDDSRLEELKATLPSPEKLPGFKMYPIDFEKDDDTNFHMDFIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLNSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTI 1057          

BLAST of ubiquitin-activating enzyme e1 vs. SwissProt
Match: gi|24418865|sp|P22314.3|UBA1_HUMAN (RecName: Full=Ubiquitin-like modifier-activating enzyme 1; AltName: Full=Protein A1S9; AltName: Full=Ubiquitin-activating enzyme E1)

HSP 1 Score: 1275 bits (3298), Expect = 0.000e+0
Identity = 629/1076 (58.46%), Postives = 777/1076 (72.21%), Query Frame = 0

Query:   66 NSPPAAKKLRLEEGAEGGPDASSPPRHKNGSANGFKPMTPDTQNGAGAPANGHNPPPAHPAGGQDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYM-KVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGA-RNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLD--NKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            +S P +KK R+  G +  P ++  P     S     P     +NG+ A                DIDE LYSRQLYVLGHEAMK++  S+VLVSGL+GLGVE+AKN+ILGGVK+VTLHD  T    DLS+QFY    DIG+NRA+V+  +LAELN YV  TA + PLVE F+  F VVVLT + L +QLR+ E     GI LVVADTRGL+ Q+F DFG    + D+NGEQP+S M+S V  D  G+VTC DE RHGFE GD V+F+EV+GM ELN + PM IKVLGPYTFSI D S F+ Y +GGI  QVK+P +++FKSL  +L++   F++TDF KF RPA +H+ F  LH++  +H R P+P +  DA + VA+A+ +N + +  V  ++  E    + A +AAGDL P+ A +GG  AQEVMKACSGKFMP+ QW+YFDALECLP D  VLTE +C    +RYDGQ+AVFG   Q+KLG QKYF+VGAGAIGCELLKNFAMIGLG    G+IIVTDMD IEKSNLNRQFLFRPWDV K KS TAAAA++ MNP + V S +NRVG DTE +Y+DDFF+ LDGVANALDN++AR YMDRRCVYYR PLLESGTLGTKGN QVVIP +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +  QYL +P F+E+T+RL G QP+E L+ V++ LV ++P  +ADCV WA   +   Y N IRQLLHNFP DQ TS+G  FWSGPKRCP  L FD +  +H+D+V++AANL A+ YG+ GS D   + T L+ V V  F P+ GV+I  +D E   QS   + D   L ++   LP      G K+  ++FEKDDD+NFHMDFIVA SNLRAENY I  ADR KSKLIAG+IIPAIATTT+ V GLV LELYK+VQGH  ++ +KNGF NLALPF  FS+P+ AP  +YY +EWTLWDRF +     NG+EMTL QF++YFK + +LEITMLSQGVSMLYSFFMP  K +ER+   M+++V  VSK+ +  +V+ALV ELCCND+ GEDV+VPYV+Y +
Sbjct:    2 SSSPLSKKRRVS-GPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEA----------------DIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPD-FVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQE--LQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTI 1057          

BLAST of ubiquitin-activating enzyme e1 vs. SwissProt
Match: gi|81889667|sp|Q5U300.1|UBA1_RAT (RecName: Full=Ubiquitin-like modifier-activating enzyme 1; AltName: Full=Ubiquitin-activating enzyme E1)

HSP 1 Score: 1271.91 bits (3290), Expect = 0.000e+0
Identity = 616/1031 (59.75%), Postives = 759/1031 (73.62%), Query Frame = 0

Query:  111 AGAPANGHNPPPAHPAGGQDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYM-KVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGA-RNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLD--NKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            +  P NG     A      DIDESLYSRQLYVLGHEAMK +  S+VLVSGL+GLGVE+AKN+ILGGVK+VTLHD  T    DLS+QFY    DIG+NRA+V+  +LAELN YV  TA + PLVE F+  F VVVLT S L EQLR+ E     GI LVVADTRGL+ Q+F DFG    + D+NGEQP+S M+S V  D  G+VTC DE RHGFE GD V+F+EV+GM +LN   P+ IKVLGPYTFSI D S F+ Y +GGI  QVK+P +++FKSL  +L++   F++TDF K+ RPA +H+ F  LH++  +H R P+P +  DA + V +A+ +N +    V  D+  E    + A +AAGDL P+ A +GG  AQEVMKACSGKFMP+ QW+YFDALECLP D   LTE +C P  +RYDGQ+AVFG   Q+KLG QKYF+VGAGAIGCELLKNFAMIGLG    G+++VTDMD IEKSNLNRQFLFRPWDV K KS TAAAA++ MNP + V S +NRVG DTE +Y+DDFF+ LDGVANALDN++AR YMDRRCVYYR PLLESGTLGTKGN QVVIP +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +  QYL +  F+E+T+RL G QP+E L+ V++ LV ++P  + DCV WA   +   Y N IRQLLHNFP DQ TS+G  FWSGPKRCP  L FD +  +H+D+V++AANL A+ YG+ GS D   + ++L+ V V  F P+ GV+I  +D E   QS   + D   L ++   LP      G K+  ++FEKDDD+NFHMDFIVA SNLRAENY I+PADR KSKLIAG+IIPAIATTT+ V GLV LELYK+VQGH  ++ +KNGF NLALPF  FS+P+ AP  +YY +EWTLWDRF +     NG+EMTL QF++YFK + +LEITMLSQGVSMLYSFFMP  K +ER+   M+++V  VSK+ +  +V+ALV ELCCND+ GEDV+VPYV+Y +
Sbjct:   34 SSVPTNGM----AKNGSEADIDESLYSRQLYVLGHEAMKMLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNSPLEEQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMVQLNGCQPIEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLAEPD-FVMTDFAKYSRPAQLHIGFQALHQFCAQHNRPPRPRNEEDATELVTLAQAVNARSPPAVQQDNVDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEDKCLPRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAANLFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQE--LQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTI 1057          

BLAST of ubiquitin-activating enzyme e1 vs. SwissProt
Match: gi|267190|sp|Q02053.1|UBA1_MOUSE (RecName: Full=Ubiquitin-like modifier-activating enzyme 1; AltName: Full=Ubiquitin-activating enzyme E1; AltName: Full=Ubiquitin-activating enzyme E1 X; AltName: Full=Ubiquitin-like modifier-activating enzyme 1 X)

HSP 1 Score: 1264.98 bits (3272), Expect = 0.000e+0
Identity = 624/1076 (57.99%), Postives = 771/1076 (71.65%), Query Frame = 0

Query:   66 NSPPAAKKLRLEEGAEGGPDASSPPRHKNGSANGFKPMTPDTQNGAGAPANGHNPPPAHPAGGQDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYM-KVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGA-RNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLD--NKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            +S P +KK R+  G +  P ++  P     S     P     +NG+ A                DIDESLYSRQLYVLGHEAMK +  S+VLVSGL+GLGVE+AKN+ILGGVK+VTLHD  T    DLS+QFY    DIG+NRA+V+  +LAELN YV  TA + PLVE F+  F VVVLT S L  QLR+ E     GI LVVADTRGL+ Q+F DFG    + D+NGEQP+S M+S V  D  G+VTC DE RHGFE GD V+F+EV+GM +LN   PM IKVLGPYTFSI D S F+ Y +GGI  QVK+P +++FKSL  +L +   F++TDF K+ RPA +H+ F  LH++   H + P+P +  DA + V +A+ +N +    V  +   E    + A +AAGDL P+ A +GG  AQEVMKACSGKFMP+ QW+YFDALECLP D   LTE +C P  +RYDGQ+AVFG  FQ+KL  QKYF+VGAGAIGCELLKNFAMIGLG    G+++VTDMD IEKSNLNRQFLFRPWDV K KS TAAAA++ MNP + V S +NRVG DTE +Y+DDFF+ LDGVANALDNI+AR YMDRRCVYYR PLLESGTLGTKGN QVVIP +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +  QYL +  F+E+T+RL G QP+E L+ V++ LV ++P  + DCV WA   +   Y N IRQLLHNFP DQ TS+G  FWSGPKRCP  L FD +  +H+D+V++AANL A+ YG+ GS D   + ++L+ V V  F P+ GV+I  +D E   QS   + D   L ++   LP      G K+  ++FEKDDD+NFHMDFIVA SNLRAENY I+PADR KSKLIAG+IIPAIATTT+ V GLV LELYK+VQGH  ++ +KNGF NLALPF  FS+P+ AP  +YY +EWTLWDRF +     NG+EMTL QF++YFK + +LEITMLSQGVSMLYSFFMP  K +ER+   M+++V  VSK+ +  +V+ALV ELCCND+ GEDV+VPYV+Y +
Sbjct:    2 SSSPLSKKRRVS-GPDPKPGSNCSPAQSALSEVSSVPTNGMAKNGSEA----------------DIDESLYSRQLYVLGHEAMKMLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTTQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSSFQVVVLTNSPLEAQLRVGEFCHSRGIKLVVADTRGLFGQLFCDFGEEMVLTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFETGDFVSFSEVQGMIQLNGCQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLPASLVEPD-FVMTDFAKYSRPAQLHIGFQALHQFCALHNQPPRPRNEEDATELVGLAQAVNARSPPSVKQNSLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEALTEEKCLPRQNRYDGQVAVFGSDFQEKLSKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEVVVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPYIQVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNIDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDSKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWGDCVTWACHHWHTQYCNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNTLHLDYVMAAANLFAQTYGLTGSQDRAAVASLLQSVQVPEFTPKSGVKIHVSDQE--LQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDISPADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHQQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTI 1057          

BLAST of ubiquitin-activating enzyme e1 vs. SwissProt
Match: gi|122066571|sp|P31254.2|UBA1Y_MOUSE (RecName: Full=Ubiquitin-like modifier-activating enzyme 1 Y; AltName: Full=Ubiquitin-activating enzyme E1; AltName: Full=Ubiquitin-activating enzyme E1 Y)

HSP 1 Score: 1210.67 bits (3131), Expect = 0.000e+0
Identity = 579/1010 (57.33%), Postives = 740/1010 (73.27%), Query Frame = 0

Query:  130 DIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAVDEQ--GIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTFE-QFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGA-RNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLD--NKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQY 1133
            DIDESLYSRQLYVLGHEAMK +  S+VL+SGL+GLGVE+AKN+ILGGVK+VTLHD       DLS+QF     DIG+NRA+++  +LAELN YV   A + PL+E F+  F VVVLT + L  QL++ E     GI LVVADTRGL  Q+F DFG    + D+NGEQP+S M+S + ++  GIVTC +++RHGFE GD ++FTEV+GM+ELN   P+ IKVLGPYTFSI D S F++Y +GGI  QVK+P ++ FK L  +L++   F++TDF K   PA +H+ F  LH++  +H R P+P +  DA++ V +A+ +N Q +     D  +     + A +AAGDL PM A  GG  AQEVMKACSGKFMP++QW+YFDALECLP       E +C P  +RYDGQ+AVFG   Q+KLG QKYF+VGAGAIGCELLKNFAMIGLG   +G+I VTDMD IEKSNLNRQFLFRPWD+ K KS TAAAA++ +NP + + S +NRVG +TE VY+DDFF+ LDGVANALDN++AR Y+DRRCVYYR PLLESGTLGTKGN QVV+P +TESYSSSQDPPEKSIPICTLKNFPN IEHT+QWARD FEG + Q   +  QYL +P F+E+T++L G QP+E L+ +   LV ++P  +ADCV WA   +   Y + I+QLLHNFP  Q TS+G +FWSGPKRCP  L FD +  +H+D+V++AANL A+ YG+ GS D   +  +L+ + V  F P+ G+RI  ++ E Q+ S   T D   L ++   LP      G K+  ++FEKDDD+NFHMDFIVA SNLRAENYGI+PADR KSKLIAG+IIPAIATTTS + GLV LELYK+VQGH  +E +KN F NLALP  +FS P+     +YY++EWTLWDRF +     +G+EMTL QF++YFK + +LE+ MLSQGVSMLYS FMP  K +ER+   M+++V  VSK+ +  +VK+LVFELCCN   G+D++VPYV+Y
Sbjct:   48 DIDESLYSRQLYVLGHEAMKHLQASSVLISGLQGLGVEIAKNIILGGVKAVTLHDQGIAQWADLSSQFCLREEDIGKNRAEISQPRLAELNSYVPVFAYTGPLIEEFLSGFQVVVLTNTPLEYQLQVGEFCHSHGIKLVVADTRGLVGQLFCDFGEEMILTDSNGEQPLSAMVSMITKENPGIVTCLEDSRHGFESGDFISFTEVQGMSELNGIGPIEIKVLGPYTFSICDTSSFSEYIRGGIVSQVKVPRKINFKPLLASLAEPE-FVVTDFAKCCHPAQLHIGFQALHQFCTQHSRPPRPHNEEDAEELVTLAQSVNAQALPAVQQDCLDIDLIRKLAYVAAGDLAPMNAFFGGLAAQEVMKACSGKFMPIRQWLYFDALECLPEHRVAFMEDKCLPHQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEDGEITVTDMDTIEKSNLNRQFLFRPWDITKLKSETAAAAVRDINPHIRIFSHQNRVGPETEHVYDDDFFQKLDGVANALDNVDARLYVDRRCVYYRKPLLESGTLGTKGNVQVVVPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTVQWARDEFEGLFKQSAENVNQYLTDPKFMERTLQLAGTQPLEVLEAIHCSLVLQRPQTWADCVTWAYQHWHTQYSHNIQQLLHNFPPAQLTSSGALFWSGPKRCPHPLTFDINNPLHLDYVMAAANLFAQTYGLGGSQDCAVVAKLLQSLPVPKFAPKSGIRIHVSEQELQSTSAT-TIDDSHLEELKTALPTPDKLLGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYGISPADRHKSKLIAGKIIPAIATTTSAIVGLVCLELYKVVQGHQQLESYKNSFINLALPLFSFSAPLAPECHQYYDQEWTLWDRFDVQGLQPSGEEMTLKQFLDYFKTEHKLEVIMLSQGVSMLYSVFMPASKLKERLDQPMTEIVSCVSKQKLGHHVKSLVFELCCNSDSGDDIEVPYVRY 1055          

BLAST of ubiquitin-activating enzyme e1 vs. SwissProt
Match: gi|353526293|sp|P52495.2|UBA1_CANAW (RecName: Full=Ubiquitin-activating enzyme E1 1)

HSP 1 Score: 1034.25 bits (2673), Expect = 0.000e+0
Identity = 514/1017 (50.54%), Postives = 718/1017 (70.60%), Query Frame = 0

Query:  129 QDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAVDEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQ-RLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTF-EQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSV-LTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGAR-NGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMN--VVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKG-SSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEA--QAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEW-TLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            Q+IDE LYSRQLYVLG EAM KM  +NVL+ GL GLG+E+AKN+ L GVKS++L+D   V++ DLS QF+ + ++IGQ R   +  KLAELN YV        + E  + +F  +V T  SL EQ++I+ IT    I  + AD +GL+ Q+F DFG  FTV D  GE+P+S ++S +++ G VT  D+ RHG +DGD+V F EVEGM +LN   P  ++VLGPY F I     + +Y KGG+  QVK+P  ++F+ L + L+    ++I+DF KFD+PA +HL F  LH ++ KHQ  LP P++  DA +    A+ L  Q   +  +D   E++  +    A GD+  + A  GG +AQEV+K CS KF P+KQW+YFD+LE LP++T     E   +P+ SRYDGQIAVFG++FQ+K+ + K F+VG+GAIGCE+LKN+AM+GLG+   G+I +TD D IEKSNLNRQFLFRP DV K KS  AA A++ MNPD+   + S+ ++VG +TE +++D F+  L+ V NALDN+EARTY+DRRCV+Y+ PLLESGTLGTKGNTQVVIPN+TESYSSSQDPPEKSIP+CTL++FPN+I+HT+ WA+ LF+G +++ P     YL +P+++E+T++ Q      TL+ + K L + +P  F DC+ WAR  F+  +++ I+QLL+NFP D  TSTG  FWSGPKR PK L+FD + + H+DF+I  ANL A +YG+K  ++   +   +L++V + PF+P+ GV IAA DAEA  QA +   + D E + K++  LP  +T  G ++T +EFEKDDD N H++FI A SN RA NYGI  AD  K+K IAG+IIPAIATTT+LV GLV LELYK+V G   +E +KNGF NLALPFI FS+PIK+P GKY  K++  +WDRF L   NG ++TL + +++F+++E L I+MLS GVS+LY+ F PPKK ++R+ + ++ +++ VSKK +P +VK L+FE+CC+D++GEDV+VPY+  KL
Sbjct:   13 QEIDEGLYSRQLYVLGKEAMLKMQNANVLIIGLNGLGIEIAKNIALAGVKSLSLYDPKPVSITDLSTQFFLSESEIGQPRDVASREKLAELNSYVPINVVDN-IDEETLLKFKCIVSTNISLEEQVKINNITHANNIGYINADIKGLFGQIFVDFGDKFTVIDQTGEEPLSGIVSDIEKNGTVTMLDDNRHGLQDGDYVKFAEVEGMPKLNEGNPHKVEVLGPYAFKIKIDESYGEYVKGGLYTQVKVPKDLSFEPLTKQLAAPE-YLISDFAKFDKPAQLHLGFQALHAFQTKHQGELPAPYNEQDATEAFRYAEELATQNPSILGEDKLDEKYLKELFYQARGDIPGVVAFYGGLIAQEVLKNCSSKFTPIKQWLYFDSLESLPSETEYPRNEENNKPIGSRYDGQIAVFGKAFQEKIANLKVFLVGSGAIGCEMLKNWAMMGLGSGPEGKIFITDNDSIEKSNLNRQFLFRPKDVGKNKSDVAALAVQQMNPDLKGKIDSKLDKVGPETEDIFDDKFWTQLNIVVNALDNVEARTYVDRRCVFYKKPLLESGTLGTKGNTQVVIPNLTESYSSSQDPPEKSIPLCTLRSFPNKIDHTIAWAKSLFQGYFAESPESVNLYLSQPNYVEQTLK-QNPDIKGTLENISKYL-NNRPYTFEDCIKWARQEFETKFNHDIQQLLYNFPPDAKTSTGAPFWSGPKRAPKPLEFDINNKDHLDFIIGGANLLAFIYGLKEPNATVDDFKKVLEQVIIEPFQPKSGVEIAATDAEAEEQANNLSGSIDDEQIRKIAASLPEPSTLAGYRLTPIEFEKDDDTNHHIEFITAASNCRALNYGIEIADAHKTKFIAGKIIPAIATTTALVTGLVCLELYKVVDGKDDIEQYKNGFINLALPFIGFSEPIKSPEGKYNNKKFDQIWDRFEL---NG-DITLQELLDHFEKEEGLTISMLSYGVSLLYASFFPPKKVKDRLGLKLTSLIKEVSKKEVPSHVKNLIFEICCDDEEGEDVEVPYICVKL 1021          

BLAST of ubiquitin-activating enzyme e1 vs. SwissProt
Match: gi|12643656|sp|O94609.1|UBA1_SCHPO (RecName: Full=Ubiquitin-activating enzyme E1 1; AltName: Full=Poly(A)+ RNA transport protein 3)

HSP 1 Score: 1022.31 bits (2642), Expect = 0.000e+0
Identity = 512/1011 (50.64%), Postives = 686/1011 (67.85%), Query Frame = 0

Query:  131 IDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAVDEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQ-RLPKPWSASDAQDFVAMAKGLNDQY-MKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLG-ARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMN--VVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEW-TLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            IDE LYSRQLYVLGHEAMK+M+QSNVL+ G KGLGVE+AKNV L GVKSVTL+D     + DLS+Q++ T  DIG  RA+V   KLAELN YV  +   E L   +++ F  VV+T +SL +QL I++ T    IA + AD+RGL+  +F DFG  F   DT+G +P++ MI+++ + G+VT  +ETRHG E+GD V FTEV+GM  LN   P  ++V GPYTFSIG V         G+  QVK+PT+++FKSL+E+L D   ++  DFGK  RP   H+AF  L  +   H+  LP+P +  DA +F    K +       V +D   E+   + +  A GDL  M+A +GG VAQEV+KA + KF P+KQ+ YFD+LE LP+  ++ +E  C+P   RYDGQIAVFG  FQ+K+     F+VGAGAIGCE+LKN+AM+G+    +G I VTDMD IEKSNLNRQFLFRP DV K KS  A+ A+  MNP +   + S + RVG ++E ++ D+FFE L  V NALDN+EAR Y+DRRCV++  PLLESGTLGTKGNTQVV+P++TESY SSQDPPEKS PICTLKNFPN+IEHT+ WARDLFEG + Q   +   YL  P+F+E +++   + P E L+ ++  LV EKP  F +C+ WARL F + ++N I+QLL NFP D  TSTGQ FWSGPKR P  L FD   R H DF+++AA+L A  YG+K  +D      +L   N  PF P+ G++I  N+ E +A       D++ L  ++  LP  ++  G ++T  EFEKDDD+N H+DFI A SNLRA NY I PADR K+K +AG+I+PA+ T+T++V+GLV LEL KLV G   +E +KNGF NLA+   TFSDPI +P  K   KE   +WDR+     N  + TL + I+YF+++E LE+TMLS GVS+LY+ F PPKK  ER+ + +S++VE ++KK + P+ K LV E+CC+D +GEDV+VP++  KL
Sbjct:   15 IDEGLYSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDLSSQYFLTEDDIGVPRAKVTVSKLAELNQYVPVSVVDE-LSTEYLKNFKCVVVTETSLTKQLEINDFTHKNHIAYIAADSRGLFGSIFCDFGENFICTDTDGNEPLTGMIASITDDGVVTMLEETRHGLENGDFVKFTEVKGMPGLNDGTPRKVEVKGPYTFSIGSVKDLGSAGYNGVFTQVKVPTKISFKSLRESLKDPE-YVYPDFGKMMRPPQYHIAFQALSAFADAHEGSLPRPRNDIDAAEFFEFCKKIASTLQFDVELD---EKLIKEISYQARGDLVAMSAFLGGAVAQEVLKATTSKFYPLKQYFYFDSLESLPSSVTI-SEETCKPRGCRYDGQIAVFGSEFQEKIASLSTFLVGAGAIGCEMLKNWAMMGVATGESGHISVTDMDSIEKSNLNRQFLFRPRDVGKLKSECASTAVSIMNPSLTGKITSYQERVGPESEGIFGDEFFEKLSLVTNALDNVEARMYVDRRCVFFEKPLLESGTLGTKGNTQVVVPHLTESYGSSQDPPEKSFPICTLKNFPNRIEHTIAWARDLFEGLFKQPIDNVNMYLSSPNFLETSLK-TSSNPREVLENIRDYLVTEKPLSFEECIMWARLQFDKFFNNNIQQLLFNFPKDSVTSTGQPFWSGPKRAPTPLSFDIHNREHFDFIVAAASLYAFNYGLKSETDPAIYERVLAGYNPPPFAPKSGIKIQVNENE-EAPETAANKDKQELKSIADSLPPPSSLVGFRLTPAEFEKDDDSNHHIDFITAASNLRAMNYDITPADRFKTKFVAGKIVPAMCTSTAVVSGLVCLELVKLVDGKKKIEEYKNGFFNLAIGLFTFSDPIASPKMKVNGKEIDKIWDRY-----NLPDCTLQELIDYFQKEEGLEVTMLSSGVSLLYANFQPPKKLAERLPLKISELVEQITKKKLEPFRKHLVLEICCDDANGEDVEVPFICIKL 1012          

BLAST of ubiquitin-activating enzyme e1 vs. SwissProt
Match: gi|74897382|sp|Q55C16.1|UBA1_DICDI (RecName: Full=Ubiquitin-like modifier-activating enzyme 1; AltName: Full=Ubiquitin-activating enzyme E1)

HSP 1 Score: 1003.43 bits (2593), Expect = 0.000e+0
Identity = 507/1014 (50.00%), Postives = 685/1014 (67.55%), Query Frame = 0

Query:  131 IDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQ-NRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGAR-NGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRD-GVRIAANDAEAQAQSQQ---ETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEK-EWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKD-GEDVDVPYVQYK 1134
            ID++LYSRQLY L HE MKK+  ++VLV GL+GLG+E+ K++ L GVKSVTL+D + V ++DLS+QFYF+P  +G+  RA     K+ +LN+YV     +  L + F+++F+VVVL    LA QL+++E      I  +  +TRG++ Q+FNDFG  FT+ DTNGE P + MIS++  D++GIVT  +E +    DGD VTF EV GM+ LN  PP  IK + P TFSIGD +    YT GG   +VK P  V FK L+  L       ITD  KF +P  +   F  +H++ +K++ +P+P +  DA   + +AKGL    +K P D+  E+  TQ +  A GD+ PM A +GG  AQEV+KACSGKF P+ Q  +FD++ECLP D   L E E QP+ SRYDGQI  FG++ Q K+ +  YF+VGAGAIGCE+LKNFAM+GLGA   G + VTDMD IEKSNLNRQFLFR  D+Q+ KS TAA A++ MNPD+NV +   RVG DTES YN++FF  LDGV NALDN+EAR YMD +CVYY  PLLESGTLGTKGNTQVV+P++TESYSSS+DPPEK IP+CTL NFPN IEHT+QWARD FEG +     +   YL  P +++          +ETL  +K  L+D +P DF  C+AWARL F+E ++N I QLL+NFP D  T+TG  FWSGPKR P  L FD    +H++F+++AANLRA  YGIK  ++ + I      V V  F P+   ++ + N+    + +QQ   +  D +  T +SQ LP  +   G K+  ++FEKDDD N H+DFI ATSNLRA NY I+PAD+ K+K IAG+IIPA+ TTT++VAG V +EL K++Q + ++E +K+ F NL +PF  F +PI AP  K  E   WTLWDRF +D     ++TL +F++ F++K  L+I+MLS  V++LY+ F   K +EER+KM +S++ ET+SKK +P   K L+ E+CCND + G+DVDVP V+YK
Sbjct:   13 IDDALYSRQLYALSHETMKKITSTSVLVVGLQGLGIEIVKDLSLAGVKSVTLYDKELVEIKDLSSQFYFSPEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPLALQLKVNEFCHANKIHFISVETRGVFGQLFNDFGEQFTITDTNGENPNAYMISSISQDKEGIVTVVEEQKLQLLDGDLVTFKEVNGMSALNDLPPQKIKTISPLTFSIGDTTNLPPYTSGGYVTEVKQPKVVDFKPLKNILESGENIFITDDFKFTQPTNLLAGFQAIHKFAEKNKHMPRPHNKEDANAVIEIAKGL----LKKPDDELDEKMITQLSFGAQGDIVPMQAILGGITAQEVLKACSGKFTPIHQLAFFDSVECLPEDLETLPEEEFQPIGSRYDGQIITFGKTLQNKIENLNYFLVGAGAIGCEMLKNFAMMGLGAGPKGLVHVTDMDTIEKSNLNRQFLFRSSDIQQLKSQTAANAVRVMNPDLNVKAYSLRVGPDTESHYNEEFFNSLDGVCNALDNVEARLYMDSQCVYYGKPLLESGTLGTKGNTQVVVPHLTESYSSSRDPPEKGIPVCTLHNFPNAIEHTIQWARDTFEGLFKNNADNVNSYLTNPAYVQSLKTQNPFVRLETLASIKASLMD-RPLDFNQCIAWARLKFEEYFNNNIEQLLYNFPKDMVTTTGTPFWSGPKRAPTPLKFDVENPLHLEFIVAAANLRAFNYGIKAETNIEVIQKQAANVIVPDFTPKKVKIQTSENEPAPSSNTQQAGGDAEDDQCDTILSQ-LPQPSEMAGYKINSIQFEKDDDTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVIQ-NKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDG----DITLKEFLDLFEKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKKPLPKDKKYLLLEICCNDTETGDDVDVPSVRYK 1015          

BLAST of ubiquitin-activating enzyme e1 vs. SwissProt
Match: gi|549145|sp|P22515.2|UBA1_YEAST (RecName: Full=Ubiquitin-activating enzyme E1 1)

HSP 1 Score: 992.645 bits (2565), Expect = 0.000e+0
Identity = 504/1020 (49.41%), Postives = 691/1020 (67.75%), Query Frame = 0

Query:  130 DIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLT-TSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAVDEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQ-RLPKPWSASDAQDFVAMAKGLNDQYMKVPID--DTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSV-LTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGA-RNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMN--VVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKG-SSDEK----EIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEW-TLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            +IDESLYSRQLYVLG EAM KM  SNVL+ GLKGLGVE+AKNV+L GVKS+T+ D + V + DLS QF+ T  DIGQ R  V   KLAELN YV             + QF VVV T T SL ++++I+E    +GI  + ++TRGL+   F D G  FTV D  GE+P + M+S ++  G VT  D+ RHG EDG+ V F+EVEG+ +LN      ++VLGP+ F IG V ++ +Y KGGI  +VK+P +++FKSL++ LS+   F+ +DF KFDR A +HL F  LH++  +H   LP+  +  DA + + +   L+ Q  +V  +  D  E    + +  A GD+  + A  GG VAQEV+KACSGKF P+KQ+MYFD+LE LP   +    E   QP+NSRYD QIAVFG  FQKK+ + K F+VG+GAIGCE+LKN+A++GLG+  +G I+VTD D IEKSNLNRQFLFRP DV K KS  AA A+ AMNPD+   + ++ ++VG +TE ++ND F+E LD V NALDN++ARTY+DRRCV+YR PLLESGTLGTKGNTQV+IP +TESYSSS+DPPEKSIP+CTL++FPN+I+HT+ WA+ LF+G ++    +   YL +P+F+E+T++ Q       L+ +   L   KP +F DC+ WARL F++ +++ I+QLL NFP D  TS G+ FWSG KR P  L+FD     H  FV++ A+LRA  YGIK   S+ K    E  +++  + +  F P   ++I  ND +    +       E + ++   LP  +T  G K+  ++FEKDDD N H++FI A SN RA+NY I  ADR K+K IAGRIIPAIATTTSLV GLV+LELYKL+   T +E +KNGF NLALPF  FS+PI +P G+Y  K++  +WDRF +      ++ L+  I +F++ E LEITMLS GVS+LY+ F PPKK +ER+ + ++++V+ V+KK IP +V  ++ E+C +DK+GEDV+VP++   L
Sbjct:   13 EIDESLYSRQLYVLGKEAMLKMQTSNVLILGLKGLGVEIAKNVVLAGVKSMTVFDPEPVQLADLSTQFFLTEKDIGQKRGDVTRAKLAELNAYVPVNVLDSLDDVTQLSQFQVVVATDTVSLEDKVKINEFCHSSGIRFISSETRGLFGNTFVDLGDEFTVLDPTGEEPRTGMVSDIEPDGTVTMLDDNRHGLEDGNFVRFSEVEGLDKLNDGTLFKVEVLGPFAFRIGSVKEYGEYKKGGIFTEVKVPRKISFKSLKQQLSNPE-FVFSDFAKFDRAAQLHLGFQALHQFAVRHNGELPRTMNDEDANELIKLVTDLSVQQPEVLGEGVDVNEDLIKELSYQARGDIPGVVAFFGGLVAQEVLKACSGKFTPLKQFMYFDSLESLPDPKNFPRNEKTTQPVNSRYDNQIAVFGLDFQKKIANSKVFLVGSGAIGCEMLKNWALLGLGSGSDGYIVVTDNDSIEKSNLNRQFLFRPKDVGKNKSEVAAEAVCAMNPDLKGKINAKIDKVGPETEEIFNDSFWESLDFVTNALDNVDARTYVDRRCVFYRKPLLESGTLGTKGNTQVIIPRLTESYSSSRDPPEKSIPLCTLRSFPNKIDHTIAWAKSLFQGYFTDSAENVNMYLTQPNFVEQTLK-QSGDVKGVLESISDSL-SSKPHNFEDCIKWARLEFEKKFNHDIKQLLFNFPKDAKTSNGEPFWSGAKRAPTPLEFDIYNNDHFHFVVAGASLRAYNYGIKSDDSNSKPNVDEYKSVIDHMIIPEFTPNANLKIQVNDDDPDPNANAANGSDE-IDQLVSSLPDPSTLAGFKLEPVDFEKDDDTNHHIEFITACSNCRAQNYFIETADRQKTKFIAGRIIPAIATTTSLVTGLVNLELYKLIDNKTDIEQYKNGFVNLALPFFGFSEPIASPKGEYNNKKYDKIWDRFDIKG----DIKLSDLIEHFEKDEGLEITMLSYGVSLLYASFFPPKKLKERLNLPITQLVKLVTKKDIPAHVSTMILEICADDKEGEDVEVPFITIHL 1024          

BLAST of ubiquitin-activating enzyme e1 vs. nr
Match: gi|388548526|gb|AFK65746.1| (ubiquitin-activating enzyme E1 [Eriocheir sinensis])

HSP 1 Score: 1360.9 bits (3521), Expect = 0.000e+0
Identity = 646/1016 (63.58%), Postives = 798/1016 (78.54%), Query Frame = 0

Query:  123 AHPAGGQDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNG-QIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            A    GQDIDESLYSRQLYVLGH+AM++MA S++L+SGL GLGVE+AKNVILGGVKSVTLHDT    + DL++QF+ T AD+G NRA  +H +L+ELN YV  +AS+ P+ + F++ F VVVLT S L EQLR+S       IAL++A T+GL+ Q+F DFG +F V DTNGE P+S M++ +  +E  +VTC DE+RHG EDGD+VTF+EV+GM ELN   P+ IKVLGP+TFSIG+ ++F+ Y +GGI  QVKMP +V FKSL+EA+ +   F++TDFGKFDRP ++H+AF TLH Y KKH   PKPWS  DA  F+ + K +N       +++  E    QFAK+  G L  M A +GG VAQEVMKA SGKF P+KQW+YFDALECLP DT+ LTE  C P  +RYD Q+AVFG+ FQ+KLG QK+FVVGAGAIGCELLKNFAM+GLGAR G Q++VTDMD IEKSNLNRQFLFRPWDVQK K+ TA AA+K MNP++N+++ +NRVG +TE +Y+D FFE LDGVANALDN+EAR YMDRRCVYYR PLLESGTLGTKGN QVV+P++TESY SSQDPPEKSIPICTLKNFPN IEHTLQWARD+FEG + Q   +A QYL +P F+E+ ++L G+QP+ETLD VK+ LVD++P  F DCV WAR  F E ++NQI QLLHNFP DQTTS+G+ FWSGPKRCP  + FD++  +H+D++I+AANL+AEVYGI+   D + +  ++ ++++  F PR GV+IA  DAEA+AQS Q   D E ++ +   +P  ++F  LK+T L+FEKDDD+NFHMDFIVA SNLRAENY IAPADR KSKLIAG+IIPAIATTT+LV+GLV LEL KLVQGH  +E+FKNGF NLALPF  FS+PIKAP  KYY+KEWTLWDRF +D     EMTL +FI YF+E+ QLEITMLSQGVSMLYSFFMPP KR ERM + MS+VV+ VSKK I P+V+ALV ELC NDKDGEDV+VPYV+Y L
Sbjct:   28 AANGAGQDIDESLYSRQLYVLGHDAMRRMASSDILISGLGGLGVEIAKNVILGGVKSVTLHDTKNAQLSDLNSQFFLTEADVGVNRATASHQRLSELNTYVPISASTNPITDDFLKNFRVVVLTDSPLEEQLRVSAFCHSHDIALIIASTKGLFGQIFCDFGESFEVVDTNGETPVSAMVAGITKEESSVVTCLDESRHGLEDGDYVTFSEVQGMTELNGCKPIMIKVLGPFTFSIGNTTQFSDYVRGGIVTQVKMPKKVQFKSLEEAMKEPE-FVMTDFGKFDRPGILHIAFQTLHAYVKKHGSPPKPWSEEDANTFLELFKEMNSAS-PAKVEELDENLVKQFAKVCGGGLVAMDAVIGGIVAQEVMKASSGKFSPIKQWLYFDALECLPEDTASLTEDVCAPTGTRYDTQVAVFGKEFQEKLGRQKFFVVGAGAIGCELLKNFAMLGLGAREGGQVVVTDMDLIEKSNLNRQFLFRPWDVQKPKADTAGAAVKKMNPEVNIIAHQNRVGPETERLYDDTFFEALDGVANALDNVEARLYMDRRCVYYRKPLLESGTLGTKGNVQVVVPHLTESYGSSQDPPEKSIPICTLKNFPNAIEHTLQWARDMFEGEFRQAAENAAQYLLDPKFMERALKLPGSQPVETLDSVKRALVDDRPQTFEDCVLWARKSFGEQFNNQIVQLLHNFPKDQTTSSGEPFWSGPKRCPHNIKFDSNVDLHLDYIIAAANLKAEVYGIEQVRDRQAVQDMVDKIDIPEFVPRSGVKIAVTDAEAEAQSNQ--VDSERISTLQSAIPAASSFGSLKLTPLDFEKDDDSNFHMDFIVAASNLRAENYDIAPADRHKSKLIAGKIIPAIATTTALVSGLVCLELIKLVQGHRKLELFKNGFVNLALPFFGFSEPIKAPTNKYYDKEWTLWDRFEVDG----EMTLEKFIKYFEEEHQLEITMLSQGVSMLYSFFMPPAKRNERMALPMSEVVKKVSKKKIEPHVRALVLELCANDKDGEDVEVPYVKYNL 1035          

BLAST of ubiquitin-activating enzyme e1 vs. nr
Match: gi|1050825249|gb|ANZ80593.1| (ubiquitin-activating enzyme E1 [Penaeus monodon])

HSP 1 Score: 1356.27 bits (3509), Expect = 0.000e+0
Identity = 658/1074 (61.27%), Postives = 806/1074 (75.05%), Query Frame = 0

Query:   65 ANSPPAAKKLRLEEGAEGGPDASSPPRHKNGSANGFKPMTPDTQNGAGAPANGHNPPPAHPAGGQDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGA-RNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            ANS P  K+ RLE                NGS N                ANG           QDIDESLYSRQLYVLGH+AM++MA S+VL+SGL GLGVE+AKNVILGGVKSVTLHDT   ++RDLS QF+ T  D+G NRA   H +LAELN YV  +AS  PL E F+++F VVVLT SSL EQ+++S       IA++VA T+GL+ Q+F DFG  F V DTNGE P+S MI+ V  +E  +VTC DE+RHG EDGD+VTF+EV+GM ELN   P+ IKVLGP+TFSIGD ++F+ Y +GGI  QVK+P + +FK+L+E++ +   F++TDF KFDRP ++H+AF TLH+YEK++   P+PW+  DA+ F+   K +N+ +    +++  E    QFAK+A+G + P+ A +GG VAQEVMKACSGKF P+ QW+YFDALECLP D S +TE  C P   RYD Q+AVFG+ FQKKLG QKYF+VGAGAIGCELLKNFAM+G+GA   G+++VTDMD IEKSNLNRQFLFRPWDVQKAKS TAAAA+K MNP +N+++ +NRVG +TE VY+D FFE LDGVANALDN+EAR YMDRRCVYYR PLLESGTLGTKGN QVV+P++TESY SSQDPPEKSIPICTLKNFPN IEHTLQWARD+FEG + Q   +A QYL +  F+E+T++L G+QPME L+ VK+ LV+++P  F DCV WAR  F E +HNQI QLLHNFP DQTTS+G+ FWSGPKRCP  L FD + ++H+D+++SAANL+AEVYGI    D   +  I+ ++ +  F PR GV+IA  DAEA+AQS Q   D E +  +   +P   +F  LK+T L+FEKDDD NFHMD+IVA SNLRAENY I PADR KSKLIAG+IIPAIATTT+L++GLVSLEL KLVQGH S+E FKNGF NLALPF  FS+PI AP  KYY+KEWTLWDRF +D     EMTL +FINYF+EK  LEITMLSQGVSMLYSFFMPP KR ERM + MS+VV+ VSKK I  +V+ALV ELC NDKDGEDV+VPYV+Y L
Sbjct:    7 ANSQPPCKRPRLEPAV-------------NGSTN--------------MAANGSQ---------QDIDESLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHDTKNTSVRDLSTQFFLTSDDVGTNRATACHSRLAELNTYVPISASISPLTEEFLKKFRVVVLTDSSLEEQMQVSSFCHSNNIAVIVAATKGLFGQIFCDFGEEFEVVDTNGESPVSAMIAGVTKEENSVVTCLDESRHGIEDGDYVTFSEVQGMTELNGCKPVKIKVLGPFTFSIGDTAQFSDYVRGGIVTQVKVPVKKSFKALEESIKEPE-FIMTDFAKFDRPGILHIAFQTLHKYEKENGSAPRPWNEEDAEKFLHAFKEMNEGH-PAKVEEFDENLVKQFAKIASGGVAPLDAVIGGIVAQEVMKACSGKFTPIYQWLYFDALECLPDDLSTITEDLCTPAGDRYDSQVAVFGRDFQKKLGSQKYFIVGAGAIGCELLKNFAMLGIGAGEGGKLVVTDMDLIEKSNLNRQFLFRPWDVQKAKSDTAAAAVKKMNPQVNIIAHQNRVGPETERVYDDSFFEPLDGVANALDNVEARLYMDRRCVYYRKPLLESGTLGTKGNVQVVVPHLTESYGSSQDPPEKSIPICTLKNFPNAIEHTLQWARDMFEGEFRQAAENAAQYLVDSRFMERTLKLPGSQPMEVLESVKRALVEDRPQSFEDCVLWARKHFAEQFHNQIVQLLHNFPKDQTTSSGEPFWSGPKRCPHPLKFDANNQLHLDYILSAANLKAEVYGITQVRDRDAVADIVNKIEIPEFVPRSGVKIAVTDAEAEAQSNQ--VDSERIANLQSAIPQATSFGALKLTPLDFEKDDDTNFHMDYIVAASNLRAENYDIPPADRHKSKLIAGKIIPAIATTTALISGLVSLELLKLVQGHNSIEKFKNGFVNLALPFFGFSEPISAPKNKYYDKEWTLWDRFEVDG----EMTLEEFINYFEEKHNLEITMLSQGVSMLYSFFMPPAKRNERMSLPMSEVVKKVSKKKIEDHVRALVLELCANDKDGEDVEVPYVKYNL 1036          

BLAST of ubiquitin-activating enzyme e1 vs. nr
Match: gi|91094331|ref|XP_966352.1| (PREDICTED: ubiquitin-like modifier-activating enzyme 1 [Tribolium castaneum] >gi|1004402317|gb|KYB28908.1| Ubiquitin-like modifier-activating enzyme 1 [Tribolium castaneum])

HSP 1 Score: 1337.4 bits (3460), Expect = 0.000e+0
Identity = 658/1065 (61.78%), Postives = 801/1065 (75.21%), Query Frame = 0

Query:   78 EGAEGGPDASSPPRHKNGSANGFKPMTPDTQNGAGAPANGHNPPPAHPAGGQDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGL-----NDQYMKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            E AEG  D  +  R  +       P++ D  N      NG     A  +  QDIDE LYSRQLYVLGH+AM++MA S+VLVSGL GLGVEVAKNVILGGVKSVTLHD    ++ DLS+QFYFT AD+G+NRA+V   +LAELN YV T A + PL   FIR+F VVVLT S LAEQ++ISEIT  + IAL+VADTRGL+AQVF DFG AFTV DTNGE P+S MI+ +  D++GIVTC D+TRHG EDGDHVTF+EV+GM ELN   P+ IKVLGPYTFSIGD + F+KY +GGIA QVKMP  ++FKSL+++L     F++TDF KFD P  +H+AF  LH+Y +KH R+PKPW+  DA +F+++AK L     ND  +   + +TF       AK+ AGDLNP+ A +GG VAQEVMKACSGKF P+ QW+YFDA+ECLP +   +TE    P  +RYDGQIAVFG  FQKKLG  KYFVVGAGAIGCELLKNFAM+G+G   GQI VTDMD IEKSNLNRQFLFRP DVQ+ KS TAA  IK MNP +N+V+ ENRVG ++E++Y+D FFE LDGVANALDN++AR YMDRRCVYYR PLLESGTLGTKGNTQVV+P +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +A QYL +P+F+E+T++L G QP+E L+ VK  LVD++P  F DCV WAR  ++E Y NQI+QLL NFP DQ TSTGQ+FWSGPKRCP+ L FD +  +H+D+V +AANL+AEVYGI  + D   I  ++++++V  F P+ GV+IA  D++  A +   + D + + ++ ++LP  A    L +T L+FEKDDD N HMDFIVA SNLRA NY I PADR KSKLIAG+IIPAIATTTS+VAGLV LELYKL +   S+  FKNGF NLALPF  FS+PI AP  +Y  KEWTLWDRF +D     EMTL++F+ YFKEK  LEITMLSQGV MLYSFFM   K +ER+ + MS++V+ VSKK + P+VKALVFELCCND DG DV+VPYV+Y L
Sbjct:    5 EIAEGSVDPPTKKRKLDSC-----PISTDMAN------NG-----ASGSSQQDIDEGLYSRQLYVLGHDAMRRMASSDVLVSGLGGLGVEVAKNVILGGVKSVTLHDEAVCSVADLSSQFYFTEADVGKNRAEVCCKQLAELNTYVPTKAYTGPLTPDFIRKFRVVVLTNSCLAEQMQISEITHASNIALIVADTRGLFAQVFCDFGDAFTVVDTNGESPVSAMIADISTDKEGIVTCIDDTRHGMEDGDHVTFSEVQGMTELNNCKPIKIKVLGPYTFSIGDTTNFSKYERGGIATQVKMPKTLSFKSLKDSLKSPE-FLMTDFAKFDHPQQLHVAFQALHKYVEKHGRVPKPWNNEDASEFLSIAKSLAVDGGNDTEVNTNLLETF-------AKVCAGDLNPINATIGGIVAQEVMKACSGKFHPIYQWLYFDAIECLPTEAGEITEESAAPKGTRYDGQIAVFGSEFQKKLGGLKYFVVGAGAIGCELLKNFAMMGIGGEGGQITVTDMDLIEKSNLNRQFLFRPHDVQRPKSGTAAKVIKKMNPSINIVAHENRVGPESENMYDDTFFESLDGVANALDNVDARIYMDRRCVYYRKPLLESGTLGTKGNTQVVVPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDNFEGLFRQSAENASQYLTDPEFLERTIKLPGVQPIEVLESVKAALVDDRPKSFEDCVVWARHHWEEQYSNQIKQLLFNFPPDQKTSTGQLFWSGPKRCPEPLTFDVNNTLHLDYVFAAANLKAEVYGIPQNRDRAYIAEVVQKIHVPEFVPKSGVKIAVTDSQ-MAMTNGSSVDLDRVGQIREELPSVAELGNLCLTPLDFEKDDDTNLHMDFIVAASNLRAANYKIPPADRHKSKLIAGKIIPAIATTTSVVAGLVCLELYKLTRSLKSLTPFKNGFVNLALPFFGFSEPIAAPKNEYCGKEWTLWDRFEVDG----EMTLSEFLEYFKEKHGLEITMLSQGVCMLYSFFMAKAKAQERLGLPMSEIVKRVSKKKLEPHVKALVFELCCNDADGNDVEVPYVKYNL 1040          

BLAST of ubiquitin-activating enzyme e1 vs. nr
Match: gi|826420147|ref|XP_012524986.1| (PREDICTED: ubiquitin-like modifier-activating enzyme 1 [Monomorium pharaonis])

HSP 1 Score: 1328.92 bits (3438), Expect = 0.000e+0
Identity = 641/1008 (63.59%), Postives = 785/1008 (77.88%), Query Frame = 0

Query:  130 DIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            +IDE LYSRQLYVLGH+AM++MA S+VL+SGL GLGVE+AKNVILGGVKSVTLHD     + DL +QFY T AD+G+NRA     +L+ELN+YV T   S PL EA+I+QF VVVLT + LAEQLRIS+IT    IAL++ADTRGL++QVF DFG  FTV DTNGE P++ MI+++  D +G+VTC D+TRHG EDGD+VTF+EV+GM ELN   P+ IKVLGPYTFSIGD S+F++Y +GGI  QVKMP  + F  L+ AL++   F++TDFGKFD P  +HLAFITLH+Y  +   LP+PW+ SDA +F+A+A+ +   Y    ++   E  +T FAK+AAGDLNPM A +GG VAQEVMKACSGKF P+ QW+YFDA+ECLPAD S LTE +C    SRYD Q+AVFG++FQ K+G+ KYFVVGAGAIGCELLKNFAMIG+GA NG + VTDMD IEKSNLNRQFLFRP DVQ++KSSTAA  +K MNPDMNV++ ENRV  +TE  YND FFEVLDGVANALDN+ AR YMDRRCVYYR PLLESGTLGTKGNTQVV+P +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +A QY+ +P F+E+T++L G QP+E L+ VK  LVDE+PS+FADCVAWAR  +QE Y NQIRQLL NFP +Q TS+GQ FWSGPKRCP+ L FD +  +H+D++++ ANL+A+VYGI  + D +E+  IL  V V  F P+ GV+IA  D++ Q  +     D E LT++ ++LP      GL +   EFEKDDD NFH+DFIVA SNLRA NY I+PADR KSKLIAG+IIPAIATTTS+VAGLV LELYKL +G   + ++KNGF NLALPF  FS+PI AP  KYY+ EWTLWDRF +      E+TL +F++YFKE   LE+TMLSQG+ MLYSFFM   K +ERM + MS+VV+ VSKK + P+V+ALVFELCCND+DG DV+VPYV+Y L
Sbjct:   65 EIDEGLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHDHAVCKLADLGSQFYLTEADVGKNRAAACCQRLSELNNYVPTRHYSGPLTEAYIQQFKVVVLTETPLAEQLRISQITHANDIALILADTRGLFSQVFCDFGKTFTVVDTNGEPPVNAMIASISHDSEGVVTCLDDTRHGMEDGDYVTFSEVQGMTELNGCEPIKIKVLGPYTFSIGDTSRFSEYVRGGIVTQVKMPKTLHFDPLKTALNEPK-FLVTDFGKFDYPQQLHLAFITLHQYVTERGTLPRPWNQSDAGEFIAIAEQVKSAYGFGDMEINDELLRT-FAKVAAGDLNPMNATIGGIVAQEVMKACSGKFHPIYQWLYFDAIECLPADRSELTEEDCCATGSRYDSQVAVFGKNFQSKIGNLKYFVVGAGAIGCELLKNFAMIGVGAENGCVTVTDMDLIEKSNLNRQFLFRPSDVQQSKSSTAARVVKGMNPDMNVIAHENRVCPETEKTYNDYFFEVLDGVANALDNVNARIYMDRRCVYYRKPLLESGTLGTKGNTQVVVPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDNFEGLFRQSAENAAQYISDPQFVERTLKLPGVQPLEVLESVKTALVDERPSNFADCVAWARCHWQEQYSNQIRQLLFNFPPEQVTSSGQPFWSGPKRCPEPLVFDVNDPLHIDYIVAGANLKAKVYGIPTNRDREEVARILATVKVPDFTPKSGVKIAETDSQVQVSNGSGNIDHERLTQLQEELPKMEELNGLTIHPQEFEKDDDTNFHIDFIVAASNLRATNYKISPADRHKSKLIAGKIIPAIATTTSVVAGLVCLELYKLTRGVRDLSLYKNGFVNLALPFFGFSEPIAAPKLKYYDIEWTLWDRFEVKG----ELTLKEFLDYFKEHHNLEVTMLSQGICMLYSFFMAKPKCQERMGLLMSEVVKKVSKKKLEPHVRALVFELCCNDEDGNDVEVPYVRYTL 1066          

BLAST of ubiquitin-activating enzyme e1 vs. nr
Match: gi|1233178518|ref|XP_022190676.1| (ubiquitin-like modifier-activating enzyme 1 [Nilaparvata lugens])

HSP 1 Score: 1327.38 bits (3434), Expect = 0.000e+0
Identity = 641/1053 (60.87%), Postives = 787/1053 (74.74%), Query Frame = 0

Query:   85 DASSPPRHKNGSANGFKPMTPDTQNGAGAPANGHNPPPAHPAGGQDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            ++  PP  K   A+G   +   +Q  A    NG +      +  ++IDE LYSRQLYVLGH+AM++MA S+VL+SGL GLGVE+AKNVILGGVKSVTLHD     + DLS+QFY + ADIG+NRA+    +L+ELN+YV T A    L + FI+QFSVVVLT SSL EQL+ISE+T    IAL+VADTRG++AQVF DFGP FTV D NGE P+S M++ +  D +G+VTC D+TRHG EDGD+V F+EVEGM ELN S P  IKVLG YTFSIGD +   +Y+ GGI  QVKMP  + FKSL+E+LS    F+ITDF KFD P+ +HLAF  LH++  KH R PKPW+ +DA +FV + K L  +      DD   +  T F+K+ +G+ NP+ A +GG VAQE+MKACSGKF P+ QW+YFDA+ECLP     L E +CQP+ +R DGQ A+FG  FQ KLG  KYFVVGAGAIGCELLKNFAM+G+GA  GQ+ VTDMD IEKSNLNRQFLFRP DVQ+AKS TAA  +K MNP+MNVV+ ENRVG +TE  Y+D FFE LDGVANALDN++AR YMDRRCVYYR PLLESGTLGTKGN+QVV+P +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +A QYL++P+FIE+T+RL G QP+E L+ VK  LVDE+P  F DCVAWAR  ++E Y NQI+QLL NFP DQ TS+GQ FWSGPKRCP  + F     +H+D+V++AANL+AEVYGI  + +  EI  I+ +V V  F P+ GV+IA  D++ Q  S     D + + ++ ++LP +   K +++T LEFEKDDD N HMDFIVA SNLRA NY IAPADR KSKLIAG+IIPAIATTTS+VAGLV +EL KL +G   +E FKNGF NLALPF  FS+PI AP  K+Y+ EWTLWDRF ++     EMTL++FI+YFK++ QLEITMLSQGV MLYSFFM   KR++R  + MS+VV  VSKK +  +VKALVFELCCND DG DV+VPYV+Y L
Sbjct:    8 NSVEPPAKKRKVASGESAVKSVSQASADMAKNGSS------SHNEEIDEGLYSRQLYVLGHDAMRRMAVSDVLISGLGGLGVEIAKNVILGGVKSVTLHDESVCTVSDLSSQFYLSEADIGKNRAEACRQQLSELNNYVPTKAYVNQLTDDFIKQFSVVVLTNSSLEEQLKISEVTHANDIALIVADTRGVFAQVFCDFGPKFTVVDVNGENPVSAMVAGISKDTEGVVTCLDDTRHGLEDGDYVKFSEVEGMVELNNSEPRKIKVLGTYTFSIGDTTNLTEYSHGGIVTQVKMPKTLTFKSLKESLSSPE-FLITDFAKFDHPSQLHLAFQALHQFISKHGRTPKPWNDADASEFVGIVKTLQSEGG----DDLNNELLTTFSKVCSGNFNPINATIGGIVAQEIMKACSGKFHPIFQWLYFDAIECLPES---LNEADCQPVGARTDGQTAIFGSQFQNKLGALKYFVVGAGAIGCELLKNFAMMGIGANGGQVTVTDMDLIEKSNLNRQFLFRPQDVQRAKSVTAARVVKKMNPNMNVVAHENRVGPETEKTYDDAFFENLDGVANALDNVDARIYMDRRCVYYRKPLLESGTLGTKGNSQVVVPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDSFEGLFKQNAENAAQYLDDPNFIEQTLRLPGVQPVEILESVKAALVDERPQSFEDCVAWARNHWEEQYSNQIKQLLFNFPQDQLTSSGQPFWSGPKRCPAPITFSVDNSLHLDYVLAAANLKAEVYGIPQNRNRDEIAQIVSKVKVPEFTPKSGVKIAVTDSQVQMGSDG-NVDNDRIGQLKRELPSREQMKTIRITPLEFEKDDDNNLHMDFIVAASNLRAANYNIAPADRHKSKLIAGKIIPAIATTTSVVAGLVCIELIKLAKGCNKLESFKNGFVNLALPFFGFSEPIAAPKLKFYDTEWTLWDRFEVEG----EMTLSEFIDYFKKEHQLEITMLSQGVCMLYSFFMDKVKRQDRFNLPMSEVVRKVSKKKLESHVKALVFELCCNDNDGNDVEVPYVRYTL 1041          

BLAST of ubiquitin-activating enzyme e1 vs. nr
Match: gi|751227542|ref|XP_011167243.1| (PREDICTED: ubiquitin-like modifier-activating enzyme 1 [Solenopsis invicta])

HSP 1 Score: 1327 bits (3433), Expect = 0.000e+0
Identity = 640/1009 (63.43%), Postives = 780/1009 (77.30%), Query Frame = 0

Query:  130 DIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQY-MKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            +IDE LYSRQLYVLGH+AM++MA S+VL+SGL GLGVE+AKNVILGGVKSVTLHD     + DL +QFY T AD+G+NRA     +L+ELN+YV T   S PL E++I+QF VVVLT + LAEQLRIS+IT    IAL++ADTRGL++QVF DFG  FTV DTNGE P+S MI+++  D +G+VTC D+TRHG EDGD+VTF+EV+GM ELN   P+ IKVLGPYTFSIGD S+F++Y + GI  QVKMP  + F SLQ AL     F+ITDFGKFD P  +HLAF+TLH+Y      LP+PW+ SDA +F+A+ + + D Y     I++   +    FAK++AGDLNPM A +GG VAQEVMKACSGKF P+ QW+YFDA+ECLP D S LTE +C    SRYD Q+AVFG++FQ K+G  KYFVVGAGAIGCELLKNFAMIG+GA NG + VTDMD IEKSNLNRQFLFRP DVQ++KSSTAA  IK MNPDM VV+ ENRV  +TE +YNDDFFEVLDGVANALDN+ AR YMDRRCVYYR PLLESGTLGTKGNTQVV+P +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +A Q++ +P F+E+T++L G QP+E L+ VK  LVDE+P++FADCVAWAR  +QE Y NQIRQLL NFP DQ TS+GQ FWSGPKRCP  L FD +  +HMD++++AANL+A+VYGI  + D +EI  IL  V V  F P+ GV+IA  D++ Q  +     D E L+++ ++LP       + +   EFEKDDD NFH+DFIVA SNLRA NY I+PADR KSKLIAG+IIPAIATTTS+VAGLV LELYKL +G   + ++KNGF NLALPF  FS+PI AP  KYY+ EWTLWDRF +      E+TL +F++YFKE+  LE+TMLSQG+ MLYSFFM   K +ERM + MS+VV+ VSKK + P+V+ALVFELCCND+DG DV+VPYV+Y L
Sbjct:   67 EIDEGLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHDHAACKLADLGSQFYLTEADVGKNRAAACCQRLSELNNYVPTRHYSGPLSESYIQQFKVVVLTETPLAEQLRISQITHANNIALILADTRGLFSQVFCDFGETFTVVDTNGEPPVSAMIASISRDSEGVVTCLDDTRHGMEDGDYVTFSEVQGMTELNGCDPIKIKVLGPYTFSIGDTSRFSEYVRSGIVTQVKMPKTLHFASLQTALKKPE-FVITDFGKFDYPEQLHLAFLTLHQYIANRGTLPRPWNQSDADEFIAIIEQIKDNYGFDTEINN---ELLRTFAKVSAGDLNPMNATIGGIVAQEVMKACSGKFHPIYQWLYFDAIECLPVDRSELTEEDCCATGSRYDSQVAVFGKNFQSKIGSLKYFVVGAGAIGCELLKNFAMIGVGAENGCVTVTDMDLIEKSNLNRQFLFRPSDVQQSKSSTAARVIKGMNPDMKVVAHENRVCPETEKIYNDDFFEVLDGVANALDNVNARIYMDRRCVYYRKPLLESGTLGTKGNTQVVVPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDTFEGLFRQSAENAAQHISDPQFVERTLKLPGVQPLEVLESVKIALVDERPNNFADCVAWARCHWQEQYSNQIRQLLFNFPPDQVTSSGQPFWSGPKRCPDPLVFDVNDSLHMDYIVAAANLKAKVYGIPTNRDREEIARILATVKVPDFTPKSGVKIAETDSQVQVSNGSGNIDHERLSQLQEELPKMEDLNSMVIYPQEFEKDDDTNFHIDFIVAASNLRATNYKISPADRHKSKLIAGKIIPAIATTTSVVAGLVCLELYKLTRGVRDLSLYKNGFVNLALPFFGFSEPIAAPKLKYYDIEWTLWDRFEVKG----ELTLKEFLDYFKERHNLEVTMLSQGICMLYSFFMAKLKCQERMGLLMSEVVKKVSKKKLEPHVRALVFELCCNDEDGNDVEVPYVRYTL 1067          

BLAST of ubiquitin-activating enzyme e1 vs. nr
Match: gi|1101381505|ref|XP_018908645.1| (PREDICTED: ubiquitin-like modifier-activating enzyme 1 [Bemisia tabaci])

HSP 1 Score: 1325.46 bits (3429), Expect = 0.000e+0
Identity = 644/1071 (60.13%), Postives = 789/1071 (73.67%), Query Frame = 0

Query:   69 PAAKKLRLEEGAEGGPDASSPPRHKNGSANGFKPMTPDTQNGAGAPANGHNPPPAHPAGGQDIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQET--TDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            P AKK ++  G     D+ S  R +              QNG+  P               DIDE LYSRQLYVLGH+AM++M  S+VL+SGL GLGVE+AKNVILGGVKSVTLHD     + DLS+QFY + + +GQNRA+ +  +L ELN YV   + + PL E FI+ F VVVLT SS  EQLRISEIT  A IAL++ADTRGL+AQVF DFG  FTV DT GE P+S MI+ +  D +G+VTC DETRHG EDGD+VTF+EV+GM ELN   P+ I+VLGPYTFSI D SKF+ Y +GG+  QVKMP  + F  L+EA+ +   ++I DF KFD P+ +H+AF  LH Y K   R PKPW+++DA +F+ ++K L+  Y     +D  E+    FAK AAG+L  M A +GG VAQEVMKAC+GKF P+ QW YFDA+ECLP D S +TE +C+P+N RYDGQ AVFG++FQKKLG  KYFVVGAGAIGCELLKNFAM+G+G+  GQ+IVTDMD IEKSNLNRQFLFRP DVQ+ KS TAA AI  MNP MNV + ENRVG +TE +YND FFE LDGVANALDNI+AR YMDRRC+YY++PLLESGTLGTKGNTQVV+P++TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +A QY+ +P+FIE+T+RL G QP+E L+ VK  LVDE+P +F DCV WAR  FQ+ Y NQIRQLL NFP+D  TSTGQ FWSGPKRCP  ++FD +  +H+D+VI+AANL+A VYGI  + D + I  ++K+V V  F P+ GV IA  DA+A  Q    +   DQ+ + ++  +LP ++    L++T L+FEKDDD NFH+DFIVA SNLRA NY I  ADR KSKLIAG+IIPAIATTTS+V GLV LEL KL QG  S+E +KNGF NLALPF  FS+PI AP  KYY+ EWTLWDRF +      EMT+ +FINYFKEK  L+ITMLSQGV MLYSFFMPP K +ER+ ++M++VV  VSKK I P+V+ALVFE  C D++G DVDVP+V+Y L
Sbjct:   13 PPAKKRKIATGEPAPADSVSEARLEMA------------QNGSDRPQT-------------DIDEGLYSRQLYVLGHDAMRRMQSSDVLISGLSGLGVEIAKNVILGGVKSVTLHDQSKCELSDLSSQFYLSESSVGQNRAEASIQQLGELNSYVPLKSYTGPLTEDFIKGFRVVVLTNSSREEQLRISEITHAANIALIIADTRGLFAQVFCDFGTEFTVVDTTGENPVSAMIAGITKDTEGVVTCLDETRHGLEDGDYVTFSEVQGMTELNNCAPIKIRVLGPYTFSISDTSKFSDYIRGGVVTQVKMPKVINFLPLKEAI-EKPEYLIADFAKFDHPSTLHVAFTALHSYIKSQGRPPKPWNSNDAAEFLKISKTLSPNY-----EDLNEKLLEIFAKTAAGNLGAMCATIGGIVAQEVMKACTGKFNPIYQWFYFDAIECLPEDLSQMTEEDCKPVNDRYDGQTAVFGRTFQKKLGALKYFVVGAGAIGCELLKNFAMMGVGSEGGQVIVTDMDLIEKSNLNRQFLFRPHDVQRPKSVTAAKAIHKMNPKMNVTAHENRVGVETEKIYNDSFFESLDGVANALDNIDARIYMDRRCIYYQIPLLESGTLGTKGNTQVVVPHLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDNFEGLFRQAAENAAQYICDPNFIERTLRLPGVQPLEILESVKTALVDERPYNFEDCVVWARHHFQDQYSNQIRQLLFNFPADSVTSTGQPFWSGPKRCPSPIEFDVNESLHLDYVIAAANLKASVYGIPVNRDRQAIAEMVKKVKVPEFTPKSGVTIAVTDAQASEQMANGSGNLDQDRIGQLQSELPSRSQLSNLRITPLDFEKDDDTNFHIDFIVAASNLRATNYSIPIADRHKSKLIAGKIIPAIATTTSVVGGLVCLELIKLAQGFKSLERYKNGFVNLALPFFGFSEPIAAPKLKYYDTEWTLWDRFEIQG----EMTMGEFINYFKEKYNLKITMLSQGVCMLYSFFMPPAKVQERLNLSMTEVVRKVSKKKIEPHVRALVFEPYCTDEEGNDVDVPFVKYTL 1048          

BLAST of ubiquitin-activating enzyme e1 vs. nr
Match: gi|861658471|gb|KMR03596.1| (ubiquitin-like modifier-activating enzyme 1-like protein [Lasius niger])

HSP 1 Score: 1324.69 bits (3427), Expect = 0.000e+0
Identity = 641/1010 (63.47%), Postives = 782/1010 (77.43%), Query Frame = 0

Query:  130 DIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTV-AMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQY-MKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            +IDE LYSRQLYVLGH+AM++MA S+VL+SGL GLGVE+AKNVILGGVKSVTLHD + + ++R+L +QFY T  D+G+NRA     +L+ELN+YV T   S  L E +++QF VVVLT +SLAEQLRIS ITR  GIAL++ADTRGL++QVF DFG AFTV DTNGE P+S M++++  D +G+VTC D+TRHG EDGD+VTF+EV+GM ELN   P+ IKVLGPYTFSIGD S+F++Y +GGI  QVKMP  + F  L+ AL     F++TDFGKFD P  +HLAF+TLH+Y      LP+ W+  DA +F+  A+ + D+Y     I+    +    FAK++AG+LNPM A +GG VAQEVMKACSGKF P+ QWMYFDA+ECLPAD S LTE EC P+ SRYD Q+AVFG+ FQ KLG+ KYFVVGAGAIGCELLKNFAMIG+GA  G + VTDMD IEKSNLNRQFLFRP DVQ++KSSTAA  IK MNP MNVV+ ENRV  +TE +YNDDFFEVLDGVANALDN+ AR YMDRRCVYYR PLLESGTLGTKGNTQVV+P +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +A QY+ +P F+++T++L G QP+E L+ VK  LVDEKPS FADCVAWAR  +QE Y NQIRQLL NFP DQ TS+GQ FWSGPKRCP  L FD +  +HMD++++ ANL+A+VYGI  + D +EI  IL  V V  F P+ GV+IA  D++ Q  +     D E LT++ ++LP       L +   +FEKDDD NFH+DFIVA+SNLRA NY I+PADR KSKLIAG+IIPAIATTTS+VAGLV LELYKL +G   + ++KNGF NLALPF  FS+PI AP  KYY+ EWTLWDRF +      E+TL +F++YFKE+  LE+TMLSQG+ MLYSFFM   K +ERM + MS+VV+ VSKK + P+V+ALVFELCCND+DG DV+VPYV+Y L
Sbjct:   88 EIDEGLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNVILGGVKSVTLHDPEYMCSLRELGSQFYLTEKDLGKNRAAACCQRLSELNNYVPTRHYSGDLTETYLQQFKVVVLTETSLAEQLRISRITRANGIALIIADTRGLFSQVFCDFGDAFTVVDTNGEPPVSAMVASISRDSEGVVTCLDDTRHGMEDGDYVTFSEVQGMIELNGCDPIKIKVLGPYTFSIGDTSRFSEYVRGGIVTQVKMPKTLRFAPLEVALKKPE-FLVTDFGKFDYPEQLHLAFLTLHQYMDNKHALPRSWNQEDADEFIYTAEKIKDKYGFDTEING---ELLGTFAKVSAGNLNPMNATIGGIVAQEVMKACSGKFHPIYQWMYFDAIECLPADCSELTEKECAPIGSRYDSQVAVFGKKFQHKLGNLKYFVVGAGAIGCELLKNFAMIGVGAEKGCVTVTDMDLIEKSNLNRQFLFRPSDVQQSKSSTAARVIKGMNPYMNVVAHENRVCPETEKIYNDDFFEVLDGVANALDNVNARIYMDRRCVYYRKPLLESGTLGTKGNTQVVVPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDSFEGLFRQSAENAAQYISDPQFVDRTLKLPGVQPLEVLESVKTALVDEKPSTFADCVAWARCHWQEQYSNQIRQLLFNFPQDQVTSSGQPFWSGPKRCPDPLIFDVNDPLHMDYIVAGANLKAKVYGILTNRDREEIAGILATVKVPEFAPKSGVKIAETDSQVQVSNGSGNIDHERLTQLQEELPKMEELNSLAIYPQDFEKDDDTNFHIDFIVASSNLRATNYKISPADRHKSKLIAGKIIPAIATTTSVVAGLVCLELYKLTRGVRDLSLYKNGFVNLALPFFGFSEPIAAPKLKYYDVEWTLWDRFEVKG----ELTLKEFLDYFKERHNLEVTMLSQGICMLYSFFMAKPKCQERMGLLMSEVVKKVSKKKLEPHVRALVFELCCNDEDGNDVEVPYVRYTL 1089          

BLAST of ubiquitin-activating enzyme e1 vs. nr
Match: gi|795117131|ref|XP_011883301.1| (PREDICTED: ubiquitin-like modifier-activating enzyme 1 isoform X1 [Vollenhovia emeryi])

HSP 1 Score: 1322.76 bits (3422), Expect = 0.000e+0
Identity = 642/1024 (62.70%), Postives = 783/1024 (76.46%), Query Frame = 0

Query:  116 NGHNPPPAHPAGGQ--DIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGLNDQYMKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            NG     A  AG    +IDE LYSRQLYVLGH+AM++MA S+VL+SGL GLGVE+AKN+ILGGVKSVTLHD     + DL +QFY T AD+G+NRA     +L+ELN+YV T   S PL E +I+QF VVVLT + L EQLRIS+ITR   IAL++ADTRGL++QVF DFG +FTV DTNGE P+S M+++V  D +G+VTC D+TRHG EDGD+VTF+EV+GM ELN   P+ IKVLGPYTFSIGD S+F++Y + GI  QVKMP  + F  LQ AL     F++TDFGKFD P  +HLAF+TLH+Y      LP+ W+ SDA +FVA+A+ + D +     +   E  +T FAK++AGDLNPM A +GG VAQEVMKACSGKF P+ QW+YFDA+ECLP D S LTE +C P  SRYD Q+AVFG+ FQ K+G  KYFVVGAGAIGCELLKNFAM+G+GA NG + VTDMD IEKSNLNRQFLFRP DVQ++KSSTAA  IK MNPDM VV+ ENRV  +TE +YNDDFFEVLDGVANALDN+ AR YMDRRCVYYR PLLESGTLGTKGNTQVV+P +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   +A QY+ +  F+++T++L G QP+E L+ VK  LVDE+P++FADCVAWAR  +QE Y NQIRQLL NFP DQ TS+GQ FWSGPKRCP  L FD +  +H+D++++ ANL+A+VYGI  + D +EI  IL  V V  F P+ GV+IA  D++ Q  +     D E L+++ ++LP      GL +   EFEKDDD NFH+DFIVA SNLRA NY I+PADR KSKLIAG+IIPAIATTTS+VAGLV LELYKL +G   + ++KNGF NLALPF  FS+PI AP  KYY+ EWTLWDRF +      E+TL +F++YFKE+  LE+TMLSQG+ MLYSFFM   K +ERM + MS+VV+ VSKK + P+V+ALVFELCCND+DG DV+VPYV+Y L
Sbjct:   48 NGSTSTSAGTAGRAPAEIDEGLYSRQLYVLGHDAMRRMASSDVLISGLGGLGVEIAKNIILGGVKSVTLHDHAACKLADLGSQFYLTEADVGKNRAAACCQRLSELNNYVPTRHYSGPLSEDYIQQFKVVVLTETPLTEQLRISQITRANDIALILADTRGLFSQVFCDFGESFTVVDTNGEPPVSAMVASVSRDSEGVVTCLDDTRHGMEDGDYVTFSEVQGMTELNGCEPIKIKVLGPYTFSIGDTSRFSEYVRSGIVTQVKMPKTLHFTPLQTALKKPE-FLVTDFGKFDYPEQLHLAFLTLHQYMADRGTLPRSWNQSDADEFVALAEQVKDSH-GFDTEINGELLRT-FAKVSAGDLNPMNATIGGIVAQEVMKACSGKFHPIYQWLYFDAIECLPVDRSELTEEDCSPTGSRYDSQVAVFGKKFQSKIGSLKYFVVGAGAIGCELLKNFAMVGVGAENGCVTVTDMDLIEKSNLNRQFLFRPSDVQQSKSSTAARVIKGMNPDMKVVAHENRVCPETEKIYNDDFFEVLDGVANALDNVNARIYMDRRCVYYRKPLLESGTLGTKGNTQVVVPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDNFEGLFRQSAENAAQYISDGQFVDRTLKLPGVQPLEVLESVKTALVDERPNNFADCVAWARCHWQEQYSNQIRQLLFNFPPDQVTSSGQPFWSGPKRCPDPLAFDVNDSLHIDYIVAGANLKAKVYGIPTNRDREEIARILATVKVPVFTPKSGVKIAETDSQVQVSNGSGNIDHERLSQLQEELPKVENLNGLAIYPQEFEKDDDTNFHIDFIVAASNLRATNYKISPADRHKSKLIAGKIIPAIATTTSVVAGLVCLELYKLTRGVRDLSLYKNGFVNLALPFFGFSEPIAAPKLKYYDIEWTLWDRFEVKG----ELTLKEFLDYFKERHNLEVTMLSQGICMLYSFFMAKPKCQERMGLLMSEVVKKVSKKKLEPHVRALVFELCCNDEDGNDVEVPYVRYTL 1064          

BLAST of ubiquitin-activating enzyme e1 vs. nr
Match: gi|1229717210|ref|XP_022120906.1| (ubiquitin-like modifier-activating enzyme 1 [Pieris rapae])

HSP 1 Score: 1322.38 bits (3421), Expect = 0.000e+0
Identity = 628/1011 (62.12%), Postives = 781/1011 (77.25%), Query Frame = 0

Query:  130 DIDESLYSRQLYVLGHEAMKKMAQSNVLVSGLKGLGVEVAKNVILGGVKSVTLHDTDTVAMRDLSAQFYFTPADIGQNRAQVAHGKLAELNHYVTTTASSEPLVEAFIRQFSVVVLTTSSLAEQLRISEITRPAGIALVVADTRGLYAQVFNDFGPAFTVYDTNGEQPISTMISAV--DEQGIVTCQDETRHGFEDGDHVTFTEVEGMAELNTSPPMPIKVLGPYTFSIGDVSKFAKYTKGGIAIQVKMPTQVAFKSLQEALSDDGLFMITDFGKFDRPALMHLAFITLHEYEKKHQRLPKPWSASDAQDFVAMAKGL---NDQYMKVPIDDTFEQFQTQFAKLAAGDLNPMAAAMGGFVAQEVMKACSGKFMPVKQWMYFDALECLPADTSVLTEGECQPLNSRYDGQIAVFGQSFQKKLGDQKYFVVGAGAIGCELLKNFAMIGLGARNGQIIVTDMDCIEKSNLNRQFLFRPWDVQKAKSSTAAAAIKAMNPDMNVVSQENRVGGDTESVYNDDFFEVLDGVANALDNIEARTYMDRRCVYYRLPLLESGTLGTKGNTQVVIPNVTESYSSSQDPPEKSIPICTLKNFPNQIEHTLQWARDLFEGTYSQGPLHAKQYLEEPDFIEKTMRLQGAQPMETLDLVKKMLVDEKPSDFADCVAWARLFFQELYHNQIRQLLHNFPSDQTTSTGQMFWSGPKRCPKALDFDTSCRVHMDFVISAANLRAEVYGIKGSSDEKEIGTILKRVNVIPFKPRDGVRIAANDAEAQAQSQQETTDQEALTKMSQDLPGKATFKGLKVTCLEFEKDDDANFHMDFIVATSNLRAENYGIAPADRLKSKLIAGRIIPAIATTTSLVAGLVSLELYKLVQGHTSVEVFKNGFANLALPFITFSDPIKAPVGKYYEKEWTLWDRFVLDNKNGQEMTLAQFINYFKEKEQLEITMLSQGVSMLYSFFMPPKKREERMKMTMSKVVETVSKKSIPPYVKALVFELCCNDKDGEDVDVPYVQYKL 1135
            +IDESLYSRQLYVLGH+AM++MA S+VLVSGL GLGVE+AKNVILGGVKSVTLHD  T  + DLS+QFY + A IGQNRA  +  +LAELNHYV TTA +EPL E F+++F VVVLT ++ AEQ R++ IT    IAL++ADTRGL++QVF DFGP FTVYD  GE P+S M++ +  + + +VTC D+TRHG EDGD+VTF+EV+GM ELN   P  IKV+GPYTFSIGD + F+KY +GGI  QVKMP ++ FK L E+L +   ++ITDF K D P  +H+AF  LH++E+     PKPW  +DA  F+   K +    + + +  ++   +  +  FAK+++GDLNPM A++GG VAQEVMKACSGKF P+ QW+Y DA+ECLP D S LTE  C+P+ SRYDGQ+AVFG+ FQ+++G+ KYF+VGAGAIGCELLKNFAMIG+GA  GQ+ +TDMD IEKSNLNRQFLFRP DVQK KSSTAA A+K MNP MNV++QENRV  +TESVY+D FFE LDGVANALDN++AR YMDRRCVYYR PLLESGTLGTKGNTQVV+P +TESYSSSQDPPEKSIPICTLKNFPN IEHTLQWARD FEG + Q   HA QYL++P F+E++M+L G QP++ L+ V+   ++++P +F DC+ WARL ++  Y NQI+QLL+NFP DQ T++G  FWSGPKRCP  L FD +  +H+D+VI+AANLRA+VYG+    D + I  +   + V  FKPR GV+IA  D  AQ Q   +  DQ+ +  +  DLP  +    LK+T L+FEKDDD+NFHMDFIVA SNLRA NY I PADR +SKLIAG+IIPAIATTTS+VAGLV LELYKL QG  S++VFKNGF NLALPF  FS+PI AP  KYY+KEWTLWDRF +      E+TL +FI+YFK   +LEITMLSQGV MLYSFFMP  KR+ER+ + MS+VV  VSK+ + P+VKALVFELCCND+ GED++VPYV+Y L
Sbjct:   42 EIDESLYSRQLYVLGHDAMRRMAHSDVLVSGLGGLGVEIAKNVILGGVKSVTLHDERTCKVSDLSSQFYLSEASIGQNRAVASCEQLAELNHYVPTTAYTEPLTEEFLQKFRVVVLTGATRAEQDRVAAITHANNIALIIADTRGLFSQVFCDFGPEFTVYDVTGENPVSAMVADITHEYEAVVTCLDDTRHGLEDGDYVTFSEVQGMTELNGCEPRKIKVMGPYTFSIGDTTTFSKYARGGIVTQVKMPKKLNFKPLSESLKEPE-YLITDFAKMDYPQQLHVAFAALHKFEQAEGYTPKPWCNADASKFIDYVKNILKSGEMFKEGEMEINTDLIEI-FAKVSSGDLNPMNASIGGVVAQEVMKACSGKFHPIVQWLYLDAIECLPKDKSALTEENCKPIGSRYDGQVAVFGKDFQRRIGELKYFIVGAGAIGCELLKNFAMIGVGAAGGQVTLTDMDLIEKSNLNRQFLFRPHDVQKPKSSTAAKAVKKMNPSMNVLAQENRVCPETESVYDDAFFEALDGVANALDNLDARIYMDRRCVYYRKPLLESGTLGTKGNTQVVVPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFRQAAEHAAQYLQDPHFLERSMKLPGTQPLDALESVRNA-INDRPLNFDDCITWARLHWEAQYSNQIKQLLYNFPPDQVTTSGAPFWSGPKRCPTPLAFDPNDNLHLDYVIAAANLRAQVYGLPPCVDREHIAKLANSIQVPDFKPRSGVKIAVTD--AQLQQNNDDMDQDRVKNIVADLPPPSKLGNLKITPLDFEKDDDSNFHMDFIVAASNLRAANYSIPPADRHRSKLIAGKIIPAIATTTSVVAGLVCLELYKLAQGFNSLDVFKNGFVNLALPFFGFSEPISAPTNKYYDKEWTLWDRFEVKG----EVTLQEFIDYFKNTHKLEITMLSQGVCMLYSFFMPKAKRQERLNLPMSEVVTKVSKRKLEPHVKALVFELCCNDEQGEDIEVPYVKYTL 1043