hypothetical protein DAPPUDRAFT_313219, maker-scaffold450_size166944-snap-gene-0.20 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. L. salmonis genes
Match: EMLSAG00000004990 (supercontig:LSalAtl2s:LSalAtl2s260:296466:299928:1 gene:EMLSAG00000004990 transcript:EMLSAT00000004990 description:"maker-LSalAtl2s260-snap-gene-3.53") HSP 1 Score: 1299.65 bits (3362), Expect = 0.000e+0 Identity = 722/1180 (61.19%), Postives = 821/1180 (69.58%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPKLNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDV-----QPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNT-SMELELDPRAVPSTSKALEPTTNYP--TAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEVPSTTNEGGSSDEEEEEDDETSTNQPQDNTSQAEQVEQ-------------------QTPAPQ-----------------------------TEKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAA 1124
MKI+RSKLKKS SEVP DC+ALID+L + E+FLREL+ IETW YGKCEL HWI+VLDL D VLE+A +A W D S KEL+LWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDV+LAVLNLLYMFS KR ++RL+ + G L + +H ++ ++ TLN + T IH+ VD TP +IM++LL Y VPE +MLLFTYLRLA SFS+Y +RLKCVQARLQALSVLIYSNQL+DNVQSLLY GLLEELVEVLEM GDHLMEIKAAALK+LTSIIHLDRNP+FPKLNTIIDVTGA+SYHGFLPVLVR+CI SLTA K+ QPFPQPLATALFSFLYHLASYEAGGEALVSCGM+ESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTH+G++ FI RLELEVEHCR+QQP+EI + SAR+D++A D E M + +M LE ST+ + TN P +A G++CLPQR+ALLKSMLNFLKKAIQD +F DSIRHVMDG LPNSLKHIISNAEYYG SLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLN RGL+AFV CKPFERLFKVLLSP YL AMRRRRSSD M DTA +LGNA+ ELMRHQP LK++AT AIIKLLEE+C +GR+ + +C KA K E VPS+ N S E +S + +++ V Q P Q + K+ VPLVDY+HNVMKFVEAILSNNSTDDHCREFV+Q+GL PLMGILGLPNLPIDFPTHQACQ+VA V K++LNL EP L QGLL LN+VL++L+PLHTPL+PPGGSVLL EL+SSP EA ++P ATPLLHHMSAAHAYIQMF++VCRTGQNDIRT+S+ HWGSELGL VL+ LSKL+ SLVWESTVLLALCS+DTLPP EFGKADMDKLLP + T+ + EET SP+SGT +SN+SVT A
Sbjct: 1 MKIDRSKLKKSSSEVPSDCKALIDRLRSTS-GDDESFLRELKGIETWTYGKCELLHWIDVLDLADRVLESAC------SASGWTLKVDGS------------------------GSLKELVLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVVLAVLNLLYMFS------------KRSNFITRLS-------PEKRGGLLTRL--NHLAASWDKN--TLNKKN----------------------------ITVIHLENVDTHPSTPGKIMEELLETYP-VPEEKRMLLFTYLRLAHSFSNYAKRLKCVQARLQALSVLIYSNQLSDNVQSLLYSGLLEELVEVLEMKGDHLMEIKAAALKSLTSIIHLDRNPNFPKLNTIIDVTGASSYHGFLPVLVRSCIASLTATKEGTSSTQQQPFPQPLATALFSFLYHLASYEAGGEALVSCGMMESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHSGLSSFIVRLELEVEHCREQQPFEIHVGSARQDAMAX-------------------DEEFLMDXSDGPTMPLE-------STNXSXTSITNPPDYSAAKTGLTCLPQRAALLKSMLNFLKKAIQDSSFGDSIRHVMDGSLPNSLKHIISNAEYYGPSLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNNRGLDAFVACKPFERLFKVLLSPVYLTAMRRRRSSDHM-DTAXSLGNAVXELMRHQPLLKEAATGAIIKLLEEVCALGRDTRIVCWKAFQKNE------VPSSNNTNRSDTNNAVEPSGSSDEEEEEDEEAXTSVSSQQQQQEQQQTPPPQGDIGDQQPQEQESSASQQHISQQQQQNQAASRATNIGHNASNKEAVPLVDYVHNVMKFVEAILSNNSTDDHCREFVQQRGLVPLMGILGLPNLPIDFPTHQACQSVAXVCKSILNLAHEPXXLKQGLLHLNEVLQSLEPLHTPLDPPGGSVLLHELISSPNXAEAXSNPQATPLLHHMSAAHAYIQMFIYVCRTGQNDIRTISVNHWGSELGLEVLKSLSKLFASLVWESTVLLALCSDDTLPPDCEFGKADMDKLLPSTATT-------------------------NDPEETLSPSSGT--ESNSSVTIA 1037
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. L. salmonis genes
Match: EMLSAG00000004991 (supercontig:LSalAtl2s:LSalAtl2s260:299952:311628:1 gene:EMLSAG00000004991 transcript:EMLSAT00000004991 description:"maker-LSalAtl2s260-augustus-gene-3.39") HSP 1 Score: 429.098 bits (1102), Expect = 1.211e-120 Identity = 195/261 (74.71%), Postives = 220/261 (84.29%), Query Frame = 0
Query: 1156 EKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPGFKPFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDS 1416
+K +K + LH QIKQIKPLL SSRLGRALAELFALLVKLCVGSP+RQRRGQ P TPA PS PA AVAS+L +LLA+GLSW PP TSPIP FRLTFF CSVGFT PMLFD+KK+PYHLMLMKF+ SGG KAFFDTFYWALSCG ++PE+GLE +LPDGTGEFLDSWL+LLE+MVNPK +LESPH LP+ F+PFDP+KY+ RTH++AF AVM +WGK PLKVYGPRM+ESVLSILCHILKGEKII EK K S
Sbjct: 16 KKDNNKYNPCLHHQIKQIKPLLXASSRLGRALAELFALLVKLCVGSPIRQRRGQHVPPTPAAPSQPAVAVASALNRLLASGLSWTPPPTSPIPGFRLTFFTCSVGFTPPMLFDDKKYPYHLMLMKFVNSGGLKAFFDTFYWALSCGGKLSPEEGLESNELPDGTGEFLDSWLMLLERMVNPKAVLESPHTLPNLSIGNFQPFDPVKYLIRTHKLAFHAVMKIWGKTPLKVYGPRMAESVLSILCHILKGEKIIAEKLSKGS 276 HSP 2 Score: 200.675 bits (509), Expect = 3.249e-51 Identity = 106/198 (53.54%), Postives = 145/198 (73.23%), Query Frame = 0
Query: 2702 AGAAGPGGVT--NHMTPAHPLLMGRQ---AGSGNDSNSNRTGTRAVQRHRASNLRGYIHLNTRGAQGNPSAPAILQSFLG-TNPQDLINQGLRR-GTPLLVDF-GFAILDSLENELPDMDGNGMFGNGGRVALSSIPSALVRWSDESRVIDGDSMHDCVTALKPDITEVVETVRDQELTERRALRKKEQEEEESVQKL 2891
+G + GG NH+TP+HPLLMGRQ G+GN NS TR++ R R ++LR Y LN+R AQ NPS PAILQ+FLG +NPQ+LI G RR G PL++D G ++LD+++N++PD+D + G+GGR ALSSIPSAL+RW++ESRVIDG+SMHDCVT LKP+I EV+E RD+ +R+K++EE + ++KL
Sbjct: 1435 SGVSTDGGANSANHVTPSHPLLMGRQNLETGTGNARNS--ASTRSLPRTRGNSLRPYFQLNSR-AQNNPSTPAILQNFLGGSNPQELITFGFRRSGAPLMMDVVGLSLLDTMDNDMPDLD-SATLGSGGRAALSSIPSALLRWNEESRVIDGESMHDCVTNLKPEIIEVIENTRDE-------VRRKKEEERKIIKKL 1621
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. SwissProt
Match: gi|73915353|sp|Q7Z6Z7.3|HUWE1_HUMAN (RecName: Full=E3 ubiquitin-protein ligase HUWE1; AltName: Full=ARF-binding protein 1; Short=ARF-BP1; AltName: Full=HECT, UBA and WWE domain-containing protein 1; AltName: Full=HECT-type E3 ubiquitin transferase HUWE1; AltName: Full=Homologous to E6AP carboxyl terminus homologous protein 9; Short=HectH9; AltName: Full=Large structure of UREB1; Short=LASU1; AltName: Full=Mcl-1 ubiquitin ligase E3; Short=Mule; AltName: Full=Upstream regulatory element-binding protein 1; Short=URE-B1; Short=URE-binding protein 1) HSP 1 Score: 1546.18 bits (4002), Expect = 0.000e+0 Identity = 795/1421 (55.95%), Postives = 996/1421 (70.09%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPKLNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKAN-SKTEAPVPAEVPSTTNEGGSSDEEEEEDDETSTNQPQDNTSQAEQVEQQTPAPQTEKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPD-IANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPG----FKPFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKD 1415
MK++R+KLKK+ +E P DC+ALID+L E L ELQ+I+TW GKCEL+HW+++LD D +L A + + +W D PE E + K LLL L+FT LLIE+SFSRHLYSS+EHLT LLASSD+ V+LAVLNLLY+FSKRSN+I RL ++KR LL+RL +LA SWGG+ENGFGLA+CC D + +P SATTL+FE++++ AE K + T+ IH+ +D++ ++P++IM+ L Y S+P+ QMLLFT++RLA FS++ +RL+ VQARL A+S+L+YSN L ++ S+LY GL+EELV+VL+++ LMEIKAA+L+ LTSI+HL+R P KL++IID TG ASYHGFLPVLVRNCI ++ D P+P ATALFSFLYHLASY+AGGEALVSCGM+E+LLKVI++ E D ITFVTRAVRV+DLITNLDM +FQ+H+G++ FI RLE EV+ CR++ P+ I+ R ++ Q+ EE ME ++D GV C+PQR+ALLKSMLNFLKKAIQDPAFSD IRHVMDG LP SLKHIISNAEYYG SLFLLAT+VVT +VFQEPSLLSSLQD GLTDV+LHALL+KDVPATREVLGSLPNVFSALCLN RGL +FV+C+PFERLFKVLLSPDYLPAMRRRRSSDP+GDTASNLG+A+DELMRHQP+LK ATTAIIKLLEE+C +GR+PKYIC K + K + A P + + + E+EE++E Q ++T Q E Q E+ P+PL+DYI NVMKFVE+ILSNN+TDDHC+EFV QKGL PL+ ILGLPNLPIDFPT ACQAVA V K++L L EP VL +GLLQL+ +L +L+PLH P+E PGGSVLLREL + + +AT ATPLLH ++AAHAYI MFVH CR GQ++IR++S+ WGS+LGLSVL LS+LY SLVWESTVLL+LC+ ++LP G EFG+ADM KL+P + G K ++ E+ G + +AS +E + D D +A ME D P +S + K K K + + +IKQIKPLLS SSRLGRALAELF LLVKLCVGSP+RQRR T P+P AR+ AS+LTKLL GLSW PP +P PRFRLTFFICSVGFT+PMLFDE+K+PYHLML KF+ SGG A F+TF WALS G V +GLEH DLPDGTGEFLD+WL+L+EKMVNP T+LESPH LP+K PG F F ++++ T + AF + LW +KPLKVYG RM+ES+L+ILCHIL+GE +I E+ K+
Sbjct: 1 MKVDRTKLKKTPTEAPADCRALIDKLKVCND---EQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVE---NMSWMLVCDR------------PERE----------QLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLHMMKYPPSATTLHFEFYADPGAEV----------KIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMY-SIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTP---KLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMD--PYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPNT------TQEGEE----------------------METDMD-----------------------GVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSTQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDSILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGG-----------------KRSDGEQ-----DGAAGSMDASTQGLLEGIGLDGDTLAPMETDE--PTASDS-------KGKSKITPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAK-LPGGVQNFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKE 1294 HSP 2 Score: 565.459 bits (1456), Expect = 9.906e-161 Identity = 386/1093 (35.32%), Postives = 589/1093 (53.89%), Query Frame = 0
Query: 1494 PHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFA---PLEKSVLDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHA-----KSEKSFENSMDHLITSPASAQAAARIHLFTLLYEDCKVLCARMVDSSKVITSMTTLLDTFQKVIKGLKDGASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVC---TRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPK----PISSEGETATKTDED---------DNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIR---TCTANASTPN-------NKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRG-GELEDDHRLILKALPPRAG--------APTSMGSLNEVSHSVIADLLNFLVQPEVESDA-------AIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRTQVIIRNSSSGDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEEDLKK----RPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL---INKSGKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPAFKNQQHTMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEATRAQGDETVEPDAEATELDISTAAESMDPNSESQ----------LQTVEEGNDEEFDEMMDQILEREG 2519
P +N L +LMDMGF RE ++A+ +T +++QAT+YLL P P GG +++D++MRAIAMSLG+++ + + S P E ++ E +E++E E+F PLE+ L F D L GC L+D LPD V++VCDL++ RNG ++ LK + +V + + L A K+ + + T P ++ A RI L TLL+E+ K+ CA +V+SS ++ + LL+ Q ++ K+ +++TPKW+TP++L ID +EK + RRA++ + W+++D SG+WCSY A+NN +ID A+ +GETS++ GRR+YT+QF +M+Q NEETGNRRPVM+ + P+ +S G+ KT E+ +N TP+ ES ET E GE + I GL + L+++CV+++G+PVD + L+A LRLCLRLT+D +A++F +L +M+LNLTQ S F GF L TLL+RH++EDP TL+HTMEKV+R T A ++T +++++Y+ R L PAACR P+ F +VA +R+ AL RG G DD L+ P A P+ + + + VI D+LN L +A + + E + G +V S+ + + +QV + +S + T Q S P S++ K+D +EE +K +PL+ S I RLLAELV+SY G A LI ++ Y G SELI E+ LA++LD LL N KD + + +A++A+ + ++AQ LV+EVK+AL RALA ES KH ++QA+ ++ST++ESCP+ + K Q + MNNI+++ LKKG++ DLAR+ H+LDLSSPN+A TVNAALKPLE L+RIVNQP +L SE A AS DS +N EA E E D + T+ +++ + MD +E+ L + E + E ++++D++LER+G
Sbjct: 1315 PQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPIM----------------------GGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAES-PEEVACRKEEEERKARE---KQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQK---EVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLNKNSKNSNGQELEKTLEESKEMDIKRKENKGNDTPL-ALESTNTEKETSLEETKIGEIL---IQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRI--ALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSPLPVIPDTIKEVIYDMLNALAAYHAPEEADKSDPKPGVMTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSINSQVFAADGASTETSASGTSQGEAS-TPEESRDGKKDKEGDRASEEGKQKGKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGAS----QDSSSNQQDPGEPGEA------EVQEEDHDVTQTEVAD-GDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDG 2360
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. SwissProt
Match: gi|341941147|sp|Q7TMY8.5|HUWE1_MOUSE (RecName: Full=E3 ubiquitin-protein ligase HUWE1; AltName: Full=E3Histone; AltName: Full=HECT, UBA and WWE domain-containing protein 1; AltName: Full=HECT-type E3 ubiquitin transferase HUWE1; AltName: Full=Upstream regulatory element-binding protein 1; Short=URE-B1; Short=URE-binding protein 1) HSP 1 Score: 1534.62 bits (3972), Expect = 0.000e+0 Identity = 791/1421 (55.67%), Postives = 991/1421 (69.74%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPKLNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKAN-SKTEAPVPAEVPSTTNEGGSSDEEEEEDDETSTNQPQDNTSQAEQVEQQTPAPQTEKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPD-IANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPG----FKPFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKD 1415
MK++R+KLKK+ +E P DC+ALID+L E L ELQ+I+TW GKCEL+HW+++LD D +L A + + +W D PE E + K LLL L+FT LLIE+SFSRHLYSS+EHLT LLASSD+ V+LAVLNLLY+FSKRSN+I RL ++KR LL+RL +LA SWGG+ENGFGLA+CC D + +P SATTL+FE++++ AE K + T+ IH+ +D++ ++P++IM+ L Y S+P+ QMLLFT++RLA FS++ +RL+ VQARL A+S+L+YSN L ++ S+LY GL+EELV+VL+++ LMEIKAA+L+ LTSI+HL+R P KL++IID TG ASYHGFLPVLVRNCI ++ D P+P ATALFSFLYHLASY+AGGEALVSCGM+E+LLKVI++ E D ITFVTRAVRV+DLITNLDM +FQ+H+G++ FI RLE EV+ CR++ P+ I+ R + Q+ EE ME ++D GV C+PQR+ALLKSMLNFLKKAIQDPAFSD IRHVMDG LP SLKHIISNAEYYG SLFLLAT+VVT +VFQEPSLLSSLQD GLTDV+LHALL+KDVPATREVLGSLPNVFSALCLN RGL +FV+C+PFERLFKVLLSPDYLPAMRRRRSSDP+GDTASNLG+A+DELMRHQP+LK ATTAIIKLLEE+C +GR+PKYIC K + K + A P + + + E+EE++E Q ++ Q E Q E+ P+PL+DYI NVMKFVE+ILSNN+TDDHC+EFV QKGL PL+ ILGLPNLPIDFPT ACQAVA V K++L L EP VL +GLLQL+ +L +L+PLH P+E PGGSVLLREL + + +AT ATPLLH ++AAHAYI MFVH CR GQ++IR++S+ WGS+LGLSVL LS+LY SLVWESTVLL+LC+ ++LP G EFG+ADM KL+P + G K ++ E+ GT+ +AS +E + D D +A ME D + S + + + +IKQIKPLLS SSRLGRALAELF LLVKLCVGSP+RQRR T P+P AR+ AS+LTKLL GLSW PP +P PRFRLTFFICSVGFT+PMLFDE+K+PYHLML KF+ SGG A F+TF WALS G V +GLEH DLPDGTGEFLD+WL+L+EKMVNP T+LESPH LP+K PG F F ++++ T + AF + LW +KPLKVYG RM+ES+L+ILCHIL+GE +I E+ K+
Sbjct: 1 MKVDRTKLKKTPTEAPADCRALIDKLKVCND---EQLLLELQQIKTWNIGKCELYHWVDLLDRFDGILADAGQTVE---NMSWMLVCDR------------PEKE----------QLKMLLLAVLNFTALLIEYSFSRHLYSSIEHLTTLLASSDMQVVLAVLNLLYVFSKRSNYITRLGSDKRTPLLTRLQHLAESWGGKENGFGLAECCRDLQMLKYPPSATTLHFEFYADPGAEV----------KIEKRTTSNTLHYIHIEQLDKISESPSEIMESLTKMY-SIPKDKQMLLFTHIRLAHGFSNHRKRLQAVQARLHAISILVYSNALQESANSILYNGLIEELVDVLQITDKQLMEIKAASLRTLTSIVHLERTP---KLSSIIDCTGTASYHGFLPVLVRNCIQAMIDPSMD--PYPHQFATALFSFLYHLASYDAGGEALVSCGMMEALLKVIKFLGDEQDQITFVTRAVRVVDLITNLDMAAFQSHSGLSIFIYRLEHEVDLCRKECPFVIKPKIQRPST------TQEGEE----------------------METDMD-----------------------GVQCIPQRAALLKSMLNFLKKAIQDPAFSDGIRHVMDGSLPTSLKHIISNAEYYGPSLFLLATEVVTVFVFQEPSLLSSLQDNGLTDVMLHALLIKDVPATREVLGSLPNVFSALCLNARGLQSFVQCQPFERLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGSAVDELMRHQPTLKTDATTAIIKLLEEICNLGRDPKYICQKPSIQKADGTATAPPPRSNHAAEEASSEDEEEEEVQAMQSFNSAQQNETEPNQQVVGTEERIPIPLMDYILNVMKFVESILSNNTTDDHCQEFVNQKGLLPLVTILGLPNLPIDFPTSAACQAVAGVCKSILTLSHEPKVLQEGLLQLDLILSSLEPLHRPIESPGGSVLLRELACAGNVADATLSAQATPLLHALTAAHAYIMMFVHTCRVGQSEIRSISVNQWGSQLGLSVLSKLSQLYCSLVWESTVLLSLCTPNSLPSGCEFGQADMQKLVPKDEKAGTTQGG-----------------KRSDGEQ-----DGTAGSMDASAQGLLEGIELDGDTLAPMETDEPSSSDSKGKSKI---------TPAMAARIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPVRQRRSHHAASTTTAPTPAARSTASALTKLLTKGLSWQPPPYTPTPRFRLTFFICSVGFTSPMLFDERKYPYHLMLQKFLCSGGHNALFETFNWALSMGGKVPVSEGLEHSDLPDGTGEFLDAWLMLVEKMVNPTTVLESPHSLPAK-LPGGVQSFPQFSALRFLVVTQKAAFTCIKNLWNRKPLKVYGGRMAESMLAILCHILRGEPVIRERLSKE 1294 HSP 2 Score: 561.992 bits (1447), Expect = 1.147e-159 Identity = 387/1100 (35.18%), Postives = 594/1100 (54.00%), Query Frame = 0
Query: 1494 PHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFA---PLEKSVLDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHA-----KSEKSFENSMDHLITSPASAQAAARIHLFTLLYEDCKVLCARMVDSSKVITSMTTLLDTFQKVIKGLKDGASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVC---TRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRP---------------KPISSEGETATKTDEDDNSPTPTPMDQSESARES--NET-VGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIR---TCTANASTPN-------NKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRG-GELEDDHRLILKALPPRAG--------APTSMGSLNEVSHSVIADLLNFLVQ---PEVESDAAIDSAEANADVNVG------GSNVPPTPAVTPGSVSNVSVTNNATNRTQVIIRNSSSGDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEEDLKK----RPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL---INKSGKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPAFKNQQHTMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEATRAQGDETVEPDAEATELDISTAAESMDPNSESQ----------LQTVEEGNDEEFDEMMDQILEREG 2519
P +N L +LMDMGF RE ++A+ +T +++QAT+YLL P P GG +++D++MRAIAMSLG+++ + + S P E ++ E +E++E E+F PLE+ L F D L GC L+D LPD V++VCDL++ RNG ++ LK + +V + + L A K+ + + T P ++ A RI L TLL+E+ K+ CA +V+SS ++ + LL+ Q ++ K+ +++TPKW+TP++L ID +EK + RRA++ + W+++D SG+WCSY A+NN +ID A+ +GETS++ GRR+YT+QF +M+Q NEETGNRRPVM+ + P K + ET K E+ + P ++ + + +E+ +ET +GE +L I GL + L+++CV+++G+PVD + L+A LRLCLRLT+D +A++F +L +M+LNLTQ S F GF L TLL+RH++EDP TL+HTMEKV+R T A ++T +++++Y+ R L PAACR P+ F +VA +R+ AL RG G DD L+ P A P+S+ + + VI D+LN L PE E+D + D VG G +V S+ + + +QV + + + T Q S P ++E K+D +EE +K +PL+ S I RLLAELV+SY G A LI ++ Y G SELI E+ LA++LD LL N KD + + +A++A+ + ++AQ LV+EVK+AL RALA ES KH ++QA+ ++ST++ESCP+ + K Q + MNNI+++ LKKG++ DLAR+ H+LDLSSPN+A TVNAALKPLE L+RIVNQP +L SE A AS DS ++ EA E E D + T+ +++ + MD +E+ L + E + E ++++D++LER+G
Sbjct: 1315 PQVNQQQLQQLMDMGFTREHAMEALLNTSTMEQATEYLLTHPPPII----------------------GGVVRDLSMSEEDQMMRAIAMSLGQDIPMDQRAES-PEEVACRKEEEERKARE---KQEEEEAKCLEKFQDADPLEQDELHTFTDTMLPGCFHLLDELPDTVYRVCDLIMTAIKRNGADYRDMILKQVVNQVWEAADVLIKAALPLTTSDTKTVSEWISQMATLPQASNLATRILLLTLLFEELKLPCAWVVESSGILNVLIKLLEVVQPCLQAAKEQK---EVQTPKWITPVLLLIDFYEKTAISSKRRAQMTKYLQSNSNNWRWFDDRSGRWCSYSASNNSTIDSAWKSGETSVRFTAGRRRYTVQFTTMVQVNEETGNRRPVMLTLLRVPRLSKNSKSSNGQELEKTLEESKETDIKHKENKGNDIPLALESTNTEKEASLDETKIGE-------IL--IQGLTEDMVTVLIRACVSMLGVPVDPDTLHATLRLCLRLTRDHKYAMMFAELKSTRMILNLTQSSGFNGFTPLVTLLLRHIIEDPCTLRHTMEKVVRSAATSGAGSTTSGVVSGSLGSREINYILRVLGPAACRNPDIFTEVANCCIRI--ALPAPRGSGTASDDEFENLRIKGPNAVQLVKTTPLKPSSLPVIPDTIKEVIYDMLNALAAYHAPE-EADKS-DPKPGGTTQEVGQLLQDMGDDVYQQYRSLTRQSSDFDTQSGFSLNSQVFAADGAPAETSTTGTSQGEAS-TPEETREGKKDKEGDRTSEEGKQKSKGSKPLMPTSTILRLLAELVRSYVGIATLIANYSYTVGQSELIKEDCSVLAFVLDHLLPHTQNAEDKDTPALARLFLASLAAAGSGTDAQVALVNEVKAALGRALAMAESTEKHARLQAVMCIISTIMESCPSTSSFYSSATAKTQHNGMNNIIRLFLKKGLVNDLARVPHSLDLSSPNMANTVNAALKPLETLSRIVNQPSSLFGSKSASSKNKSEQDAQGAS----QDSSSHQQDPGEPGEA------EVQEEDHDVTQTEVAD-GDIMDGEAETDSVVIAGQPEVLSSQEMQVENELEDLIDELLERDG 2360
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. SwissProt
Match: gi|332278235|sp|Q8H0T4.3|UPL2_ARATH (RecName: Full=E3 ubiquitin-protein ligase UPL2; Short=Ubiquitin-protein ligase 2; AltName: Full=HECT-type E3 ubiquitin transferase UPL2) HSP 1 Score: 129.413 bits (324), Expect = 3.857e-28 Identity = 130/516 (25.19%), Postives = 225/516 (43.60%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGA-PPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSME-HLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPI-QTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLY--PGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPKLNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLI---TNLDMQSFQTHAGMTCFINRLELEV 508
MK+ R + SEVP I+ +T+ + L + W + K + HW+++ D E + D +E + + PP P K+ +L L L++E+ ++ Y+S E HL+ LLAS+D DV+ A L L F KR + + L +L LA WGG+E G GL C ++H Q F + TL FE+++ + S P G + IHV V ++ ++++ L++ + +VP + + L T LR A +FS R + RL A VL+ ++ T+NV S P + ELV ++ +I+ L++L ++ DR + T++ + + G L L++ I S+ N + A AL S + L S +G A+ G++ +L+ +I+ T+ H+ V+ AV ++++ +N F+ G+ I RL+ EV
Sbjct: 1 MKLRRRR----ASEVPTKISLFINSVTSVPLELIQEPLASFR----WEFDKGDFHHWVDLFYHFDTFFEKHVKV-----------RKDLRIEEEFDESDPPFP---------------KDAVLQVLRVIRLVLENCTNKQFYTSYEQHLSLLLASTDADVVEACLQTLAAFLKRPTGKYSI---RDASLNLKLFSLAQGWGGKEEGLGLTSCATEHSCDQLFLQLGCTLLFEFYASDESPSELPGGLQV---------------IHVPDVSMRSESDLELLNKLVIDH-NVPPSLRFALLTRLRFARAFSSLATRQQYTCIRLYAFIVLVQASGDTENVVSFFNGEPEFVNELVTLVSYEDTVPAKIRILCLQSLVALSQ-DRT----RQPTVLTAVTSGGHRGLLSGLMQKAIDSVICN---TSKWSLAFAEALLSLVTVLVSSSSGCSAMREAGLIPTLVPLIK--DTDPQHLHLVSTAVHILEVFMDYSNPAAALFRDLGGLDDTIFRLKQEV 453 HSP 2 Score: 106.301 bits (264), Expect = 3.737e-21 Identity = 81/279 (29.03%), Postives = 127/279 (45.52%), Query Frame = 0
Query: 631 LPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSK-ANSKTE-APVPAEVPSTTNEGGSSDEEEEEDDETSTNQPQDNTSQAEQVEQQTPAPQTEKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPI 907
LP L I A+ +G +F LA V++ + ++P+ ++L GLT L A+ +V + E + +P ALCLN GL A + K+ SP YL A+ DT +L + +DEL+RHQ SL+ I++L + +G + SK A+ T+ APVP E+ SDE E P +TS A +E P D + NV + E +L N C FV++KG++ ++ + LP +P+
Sbjct: 521 LPECLCIIFRRAKDFGGGVFSLAATVMSDLIHKDPTCFNALDSAGLTSAFLDAI-SDEVICSAEAITCIPQCLDALCLNNSGLQAVKDRNALRCFVKIFSSPSYLKAL--------TSDTPGSLSSGLDELLRHQSSLRTYGVDMFIEILNSILIIGSGMEATTSKSADVPTDAAPVPMEIDVDEKSLAVSDEAE----------PSSDTSPA-NIELFLP------------DCVCNVARLFETVLQNAEV---CSLFVEKKGIDTVLQLFSLPLMPL 764 HSP 3 Score: 81.2629 bits (199), Expect = 1.501e-13 Identity = 53/164 (32.32%), Postives = 81/164 (49.39%), Query Frame = 0
Query: 1906 TPMDQSESARE---SNETVGEPNPNGEEVLSTIDG-LALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQ 2065
+P D + + ++ S+ GE N E + G L +EE +L LI V + AVL+LC RLT+ AI F + GG+ L NL ++ FPG+ ++A+++VRH++EDP TL+ ME IR + +AP R P FM+
Sbjct: 1511 SPQDSTAALKDALSSDVAKGESNQALESMFGKSTGYLTMEESSKVLLIACGLIKQRVPAMIMQAVLQLCARLTKSHALAIQFLENGGLSSLFNLPKKCFFPGYDTVASVIVRHLVEDPQTLQIAMETEIRQTLSGKRHIGRVLPRTFLTTMAPVISRDPVVFMK 1674
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. SwissProt
Match: gi|172045749|sp|Q8GY23.3|UPL1_ARATH (RecName: Full=E3 ubiquitin-protein ligase UPL1; Short=Ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase UPL1) HSP 1 Score: 129.413 bits (324), Expect = 4.165e-28 Identity = 132/518 (25.48%), Postives = 227/518 (43.82%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEA---AARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSME-HLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPI-QTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLY--PGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPKLNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLI---TNLDMQSFQTHAGMTCFINRLELEV 508
MK+ R + SEVP ++ I+ +T+ + E W + K + HW+++ + D E + LH E E PP P K+ +L L +++E+ ++H YSS E HL+ LLAS+D DV+ A L L F KR I + + + L S+L LA WGG+E G GL C +++ Q + TL+FE++ + S P G + IHV V ++ ++++ L++ + +VP + + L T +R A +FS R + RL A VL+ ++ T+NV S P + ELV ++ +I+ L +L ++ DR + T++ + + G L L++ I S+ + A AL S + L S +G A+ G++ +L+ +I+ T+ H+ V+ AV +++ +N F+ G+ I RL+LEV
Sbjct: 1 MKLRRRR----ASEVPSKIKSFINSVTSVPLE----LIHEPLACFRWEFDKGDFHHWVDLFNYFDTFFEKHVQVRKDLHIE-------------ENFEESDPPFP---------------KDAVLQVLRVIRVVLENCTNKHFYSSYEQHLSLLLASTDADVVEACLQTLAAFLKRQ--IGKYSI-RDASLNSKLFSLAQGWGGKEEGLGLTSCAAENSCDQVSLQLGRTLHFEFYPSDESPSELPGGLQV---------------IHVPDVSICAESDLELLNKLVIDH-NVPPSLRFALLTRMRFARAFSSLATRQQFTCIRLYAFVVLVQASGDTENVVSFFNGEPEFVNELVTLVSYEDTVPEKIRILCLLSLVALSQ-DRT----RQPTVLTAVTSGGHRGLLSGLMQKAIDSVVCI---TSKWSLAFAEALLSLVTVLVSSSSGCSAMREAGLIPTLVPLIK--DTDPQHLHLVSAAVHILEAFMDYSNPAAALFRDLGGLDDTIFRLKLEV 453 HSP 2 Score: 109.768 bits (273), Expect = 3.574e-22 Identity = 78/279 (27.96%), Postives = 124/279 (44.44%), Query Frame = 0
Query: 631 LPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANS--KTEAPVPAEVPSTTNEGGSSDEEEEEDDETSTNQPQDNTSQAEQVEQQTPAPQTEKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPI 907
LP L I A+ +G +F LA V++ + ++P+ ++L GLT L A+ +V + E + +P ALCLN GL A + K+ SP YL A+ GDT +L + +DEL+RHQ SL+ I++L + +G + SK+ + APVP E+ SDE E D + N +E P D + NV + E +L N C FV++KG++ ++ + LP +P+
Sbjct: 519 LPECLCIIFRRAKDFGGGVFSLAATVMSDLIHKDPTCFNALDSAGLTSTFLDAI-SDEVICSAEAITCIPQCLDALCLNNSGLQAVKDRNALRCFVKIFTSPSYLRAL--------TGDTPGSLSSGLDELLRHQSSLRTYGVDMFIEILNSMLIIGSGMEATTSKSADVPTSAAPVPMEIDVDEKSLAVSDEAEPSSDTSPAN-----------IELFLP------------DCVCNVARLFETVLQNAEV---CSLFVEKKGIDAVLQLFSLPLMPL 762 HSP 3 Score: 82.8037 bits (203), Expect = 5.539e-14 Identity = 57/188 (30.32%), Postives = 89/188 (47.34%), Query Frame = 0
Query: 1903 PTPTPMDQSESARE---SNETVGEPNPNGEEVLSTIDG-LALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRV------DLALLNK 2080
P+ +P D + + ++ S+ GE N E + G L +EE L LI V + AVL+LC RLT+ AI F + GG+ L NL ++ FPG+ ++A+++VRH++EDP TL+ ME IR + +AP R P FM+ ++ D +L+K
Sbjct: 1506 PSDSPQDSTAALKDALSSDVAKGESNQALELIFGKSTGYLTMEEGHKALLIACGLIKQHVPAMIMQAVLQLCARLTKSHALAIQFLENGGLSSLFNLPKKCCFPGYDTVASVIVRHLVEDPQTLQIAMETEIRQTLSGKRHIGRVLPRTFLTTMAPVISRDPVVFMKAVASTCQLESSGGRDFVILSK 1693
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. SwissProt
Match: gi|50400805|sp|O13834.1|PTR1_SCHPO (RecName: Full=E3 ubiquitin-protein ligase ptr1; AltName: Full=HECT-type E3 ubiquitin transferase ptr1; AltName: Full=Poly(A)+ RNA transport protein 1) HSP 1 Score: 103.605 bits (257), Expect = 2.675e-20 Identity = 61/201 (30.35%), Postives = 110/201 (54.73%), Query Frame = 0
Query: 599 RSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTM--GRNP-KYICSK 796
R LLK++L F+ IQ +R+++D L +SL ++ + E YG++LF T++++ ++ EP+ + +K L+ L A + + + + + + S+P F A+CLN++G + F+E P +LF + S ++ ++ D A+ LG +DELMRHQPSLK I K +++ + NP +YI +K
Sbjct: 495 RYFLLKNLLKFVLHLIQSGGSVVELRNLIDSSLISSLAFLLEHHEVYGSNLFASTTNIMSTFIHNEPTCYGIIHEKKLSHAFLDA-VNRKILNSSDAITSIPLAFGAICLNSQGFDLFLEKNPIPQLFSIFTSLNHCKSLIS-------SDNAAILGTYIDELMRHQPSLKDPIVKMIFKACDQVSALLDNFNPFQYINAK 687
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. SwissProt
Match: gi|50401412|sp|Q03280.1|TOM1_YEAST (RecName: Full=E3 ubiquitin-protein ligase TOM1; AltName: Full=HECT-type E3 ubiquitin transferase TOM1; AltName: Full=Suppressor of snRNA protein 2; AltName: Full=Temperature-dependent organization in mitotic nucleus protein 1) HSP 1 Score: 82.4185 bits (202), Expect = 5.746e-14 Identity = 54/171 (31.58%), Postives = 84/171 (49.12%), Query Frame = 0
Query: 621 DSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPK 791
D IR+++D P+ SLK I+ N +G +L DVV + EP++ L + GL V+ K + + E+L LP+V SA+CLN GL E LF LL D+ + GD ++ G +DEL RH P LK + A+ ++ ++ + RN +
Sbjct: 529 DRIRNLIDSPILVSLKKILENKLVFGLTLITYTLDVVQKVINSEPTIYPVLVEAGLIPYVIDN-FPKLIGPSAELLSLLPDVVSAICLNPEGLKQVKEKGLINNLFDFLLDADHARIL-------TGGDRSTEYGTDIDELARHYPDLKANIVEALCNVIRKMPSTFRNER 691
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. SwissProt
Match: gi|442570058|sp|Q756G2.2|TOM1_ASHGO (RecName: Full=Probable E3 ubiquitin-protein ligase TOM1; AltName: Full=HECT-type E3 ubiquitin transferase TOM1) HSP 1 Score: 77.7962 bits (190), Expect = 1.659e-12 Identity = 49/149 (32.89%), Postives = 75/149 (50.34%), Query Frame = 0
Query: 621 DSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLK 769
D IR+++D P+ + I+ N +G +L A DVV + EP++ L + G +L + + T ++L LP V SA+CLNT GL E + LF+V+ +P++ + D A N G A+DEL RH P LK
Sbjct: 529 DRIRNLIDSPILLAFNKILENRPVFGYTLVSHALDVVQTIINTEPTIYQVLVESGTIPYILQNF-DQFLGPTSDLLCMLPEVISAICLNTDGLKQVKEKNLLKYLFQVIKTPEFAKIL-------SWEDQAVNYGVALDELARHYPELK 669
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. SwissProt
Match: gi|597439636|sp|Q9P4Z1.4|TOM1_NEUCR (RecName: Full=E3 ubiquitin-protein ligase TOM1-like; AltName: Full=HECT-type E3 ubiquitin transferase TOM1-like) HSP 1 Score: 64.6994 bits (156), Expect = 1.549e-8 Identity = 74/297 (24.92%), Postives = 134/297 (45.12%), Query Frame = 0
Query: 694 EVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKA-NSKTEAPVPAEVPSTTNEGGSSDEEEEEDDETSTNQPQDNTSQAEQVEQQTPAPQTEKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKG-LEPLMGILGLPNLPIDFPTHQACQA-VAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTP--LEPPGGSVLLRELVSSPVIGEATTDPLATP 985
E + +P V +++ LN RG+ + + ++ S ++ M + DP + AS +G+++DEL RH P+L+ + AII ++ + + + +KA ++ EAP + P E + E + + E +T+ ++A P P + YI + F++ I+SN+ H + + G +E L+ + P+LP DF AC+ VAA+S+ + + + + L L ++ + +K LQP P EP L SPV E TP
Sbjct: 821 ESMNIIPTVLNSISLNNRGMKMVLSSGAIQSYMEIFESATHVQCM----AHDP--ELASTIGSSLDELSRHHPALRPAIANAIIDMIARVTHLVKT--MDATKACGARLEAPESSASP--VAEPAQATEVKGKGKEKATDDTDVEMAEASSSSSGNNKPAQ----APSIPYIQVLSTFLQPIISNS----HLKGALISAGVIEILLDLAESPSLPHDFGETPACRMLVAAISQLIES--AQIVGLPSLLFRMENTVKVLQPRINPTTTEPFFAPFLTLNSSVSPVQDEEPASEKRTP 1097
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Match: gi|1022762020|gb|KZS07874.1| (E3 ubiquitin-protein ligase HUWE1 [Daphnia magna]) HSP 1 Score: 2448.31 bits (6344), Expect = 0.000e+0 Identity = 1353/2580 (52.44%), Postives = 1717/2580 (66.55%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPKLNTIIDVTGAASYHGFLPVLVRNCITSLTAN--KKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEVPSTTNEGGSSDEEEEEDDETSTNQPQDNTSQAEQVEQQT-PAPQTEKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPD-IANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPGFK-PFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAYNLPPTSQPGGGGTSGAATSSVATSIAAATGGSNTSGAGPSGLAAAPLPQLPPPHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSVLDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHAKSEKSFENSMDHLITSPASAQAAARIHLFTLLYEDCKVLCARMVDSSKVITSMTTLLDTFQKVI---KGLKDGASTVQIE----TPKWMTPMVLFIDLHEKVILGINRRAKLKDVCTRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPKPISSEGETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRLILKALPPRAGAPTSMGSLNEVSHSVIADLLNFL---VQPEVESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRTQVIIRNSSSGDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEE-DLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL--INKSG-KDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMP---AFKNQQHTMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQP--GTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEATRAQGDETVEP-DAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILEREGNPEILAEVIEH---SIIEGDDTQHDSQMITQDESQF 2552
MKI+RSKLKKS SEVP DC+ALI++L + + + L EL++IETW YGKCEL+HW++VLDLCD +LE R H E +W + D Q+ KELLLWTLHFTTLLIEHSFSRHLY+SMEHLT++L+S+ L+V+L VLNLLYMFSKRSNFI+RL +E+R LLSRLTYLA SWGG+ENGFGLA+CC D P+ ++P SATTL+FE+++E + ++ + T IHV V+ + ++PAQ+M++L + VP QM LFT++RLA +FSDY +RL+CVQARLQALSVLIY N L D SLLY GLLEELV+VLE++ LMEI+AAAL+ LT+IIHLDRNP+FPKLNTIIDVTGAA YHGFLPVLVR+CI SLT ++DV PFP PLATALFSFLYHLASYEAGGEALV+CGM+ESLL+V+ WP +EL+HITFVTRAVRVIDLITNLDM +FQ H G+ FIN ++ CR++QPYEIR + AAAA+ A ++ G+ + + E+E+D A +S A +P T P+PGV+CLPQR+ALLKSMLNFLKKAIQD +FSDSIRHVMDG LP +LKHIISNAEYYG SLFLL TDVVT YVFQEPSLLSSLQD GLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGL +FV CKPF+RLFKVLLSPDYLPAMRRRRSSDPMGDTA+NLGNAMDELMRHQPSL+ AT II+LLEELC +G +P YI S K EA + E +EE+DE N + +E +T Q+EK PVPLVDY+ NVMKFV+AILSNNSTDDHCREFV Q+GL PLMGILGLPNLP+DFP ACQAVAAV K++LNL EPLVL QGL L VL LQ LH PL+PPGGSVLLREL SS EA T P TPLLH M+AAHAYI MFVHVCRTGQ+DIRT+SI HWGS +GL VL+GLSKLYTSLVWESTVLLALCS++TLP G +FG+AD++KLLP P K+ + S +G +N SV AA++ LST+ A MEVD + + +K + ++K +KPLLS +SRLGRALAELF LLVKLCVGSP+R RR P TP PSP A+AVA +LT+LL +GL+W+PP TSP P+FRLTF ICSVGFT+PMLFDEKK PYHLML KF++ GGQ AFFD FYWALS G V + GLE+ DLP+GTGEFLD+WL+LLEKMVNPK IL+SPH LP+K G K PF P+KY+ THR AF+AVM LWGK+PLKVYG RM+ESVL+ILCHILKGE II+EK ++ P+ S + + SS N T+ P G ++ A + + ++ G + P + A L +NP HL LMDMGFPR++C++A+ T SL+QATDYLL+ P P+ + G + A E++ G+ D+D +MRAIA+SL + + + + K E E M E PL K VLD F AL GCLSL+D+LP+ V++VC L+VA+ RNG ++S L SL +E+G + L DH + + D +I S + +A+ RIHLFTLL E+ K+ CAR ++ ++ + LL + Q+V+ + +G ++ TPKW+ P++L +DL+EK+ LG RRA ++ V + WK++DI SGKWC Y AANNK IDDAFWAG+ S+++ GRR+Y +QF SM+Q NEETGNRRP+M+ +K++PK + + ++ D+N+ MD E+ +ES P G S + GL L++ L++SCV L+ IP D + L+A +RLCLRLT+ + HA F LGG+K LLNL+Q S F GF +LATLL+RH++E+P+TL HTMEKVIR T + P+ K+ H+L R LAPAACR + F +V K LR DL+L NKRG E+D+RL++KALPPR G S+ L+ V+ ++ DLL+ L V PE E+ + ++E + V VPP +V R Q +IRNSSS DL ++ + + S K++ ST +EE D ++RPLL KSAICRLLAELV+SY GCA+L+ +H++ + SEL+ EET ALA++LD LL +G KD +V+TLVA++ASCN+ EAQ+ LV+EVK AL RAL PESN KH ++Q+LT L+STMIESCPA Q P AFK Q MNN+VK MLK+G++ DLARI H LD+SSP +A TVNAALKPLE L+RIVN P +A RP K KS + P S A+ T+ S+ TRAQ E DAE TE D+S A ES++ +++ EG E D ++ I+++ + A ++ S +E DD+ HDSQM T +ES+F
Sbjct: 1 MKIDRSKLKKSSSEVPADCKALIERLKSCSE---DELLVELKKIETWTYGKCELYHWVDVLDLCDSILE---RACHKEHENSWVLTCDLPQNKQL----------------------KELLLWTLHFTTLLIEHSFSRHLYNSMEHLTSMLSSNCLNVVLGVLNLLYMFSKRSNFISRLASERRVSLLSRLTYLAESWGGKENGFGLAECCKDLPLSSYPSSATTLHFEFYAESMGDGSGRQSGSG------KSSGSFVTVIHVEHVEWLNKSPAQLMEEL-IELHHVPLDKQMQLFTHIRLAHAFSDYRRRLQCVQARLQALSVLIYCNALADAAPSLLYAGLLEELVDVLELTEPQLMEIRAAALRTLTAIIHLDRNPNFPKLNTIIDVTGAALYHGFLPVLVRSCIASLTGQHTEQDVVPFPLPLATALFSFLYHLASYEAGGEALVACGMMESLLRVVSWPGSELEHITFVTRAVRVIDLITNLDMHAFQAHGGLQIFIN-----LDACRKEQPYEIRPPVS-----------------AAAASNAGVNIPGDEATNESESEMEVDIGATSISSSAGQPLTE----PIPGVTCLPQRAALLKSMLNFLKKAIQDASFSDSIRHVMDGSLPAALKHIISNAEYYGPSLFLLGTDVVTVYVFQEPSLLSSLQDSGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLASFVACKPFQRLFKVLLSPDYLPAMRRRRSSDPMGDTATNLGNAMDELMRHQPSLRTDATAGIIQLLEELCALGHDPNYIASSRTQKFEAAQSTSARTGPVEATGGGSSDEEEDEEEEATSSSNPAPPRGIETETGTVVQSEKTPVPLVDYVLNVMKFVDAILSNNSTDDHCREFVSQRGLIPLMGILGLPNLPVDFPVTPACQAVAAVCKSILNLAHEPLVLKQGLDHLQTVLNTLQQLHKPLDPPGGSVLLRELASSSNPTEAITSPNTTPLLHAMAAAHAYIMMFVHVCRTGQSDIRTLSINHWGSPIGLPVLKGLSKLYTSLVWESTVLLALCSDETLPLGCQFGRADLEKLLP-------------------------PELKAVSEGPSCSGVTGMELGTN-SVAAALQALSTEASPPAAMEVD-------HDQLDAKDRPRSEKLTPAQQYRMKMVKPLLSTASRLGRALAELFGLLVKLCVGSPIRHRRAHQAPPTPIVPSPSAQAVAGALTQLLTSGLAWEPPVTSPTPKFRLTFLICSVGFTSPMLFDEKKLPYHLMLHKFVSCGGQAAFFDAFYWALSAGGRVPLDQGLEYADLPEGTGEFLDAWLMLLEKMVNPKNILDSPHQLPNKALHGSKQPFSPLKYLISTHRQAFDAVMKLWGKRPLKVYGARMTESVLTILCHILKGEVIIQEKLTQEKAEEPAAPSGVATSASSGDN--------VTTTSPAVGTSNPAIAALGEAATGSSMGARRPTVDRPGAESDADL-------VNPQHLQALMDMGFPRDQCVEALLFTTSLEQATDYLLSTP-PSLLRAAQGAVAAAAAEAVSTAMDTDGN-------DEDAVMRAIALSLTAD----------------SDRTDETVAVEK----EHHEHMLYSE--PLSKQVLDQFVQQALDGCLSLLDSLPETVYRVCSLVVAMVQRNGEAYRDSMLASLAKEIGNCVTSLQDHLDGVDNLVSKADAVIESSDAMKASVRIHLFTLLCEEMKMACARQLEEKDLVLKLIRLLSSGQQVLAVKAKVNEGIDCKSVDGIGITPKWIAPLLLCLDLYEKMSLGTRRRASMEKVTSHVWKWFDIVSGKWCPYTAANNKIIDDAFWAGQNSVRVTAGRRRYLLQFSSMIQVNEETGNRRPMMLSLKEKPKEAAQDESSSV----DENA----AMDVDENEKES--------PKG----SPLSGLNLDQGSELIKSCVGLLCIPADPDTLHAAMRLCLRLTRHYQHAEKFASLGGVKQLLNLSQSSTFSGFLTLATLLLRHLVEEPETLHHTMEKVIRAYTGPNAAPSTKEFHFLMRVLAPAACRDVQLFSEVCKTFLRADLSLTNKRGD--EEDNRLLMKALPPRPGC--SLPQLSGVAREIVCDLLDALTIPVPPE-ETATPLPTSEGDGATPV---TVPPARRFAGRNV-----------RQQELIRNSSSSDLLNHEAEETVDDASRRDSLARKKE---STSSEEMDRRRRPLLPKSAICRLLAELVRSYAGCARLVAEHIFPAKTSELVSEETSALAFLLDNLLPSCQTAGDKDSPALVRTLVASLASCNHAPEAQSCLVAEVKGALNRALTMPESNDKHNRLQSLTALLSTMIESCPATGLQPQQPLTTAFKQQNLGMNNMVKFMLKRGLVNDLARIPHCLDMSSPLMAFTVNAALKPLEALSRIVNLPPAAPVAGRP-VTKPKSGDEGEPQESSAQL--------QGTSTSDNTRAQAVEISNALDAENTEQDVSAAGESLENLADN------EGGGESGDNNLEDIMDQHSQALVEALIVAAENPSQMETDDSAHDSQMRTHNESEF 2384
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Match: gi|321475415|gb|EFX86378.1| (hypothetical protein DAPPUDRAFT_313219 [Daphnia pulex]) HSP 1 Score: 2434.83 bits (6309), Expect = 0.000e+0 Identity = 1347/2580 (52.21%), Postives = 1692/2580 (65.58%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPKLNTIIDVTGAASYHGFLPVLVRNCITSLTAN--KKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEVPSTTNEGGSSDEEEEEDDETSTNQPQDNTSQAEQVEQQTPA-PQTEKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPD-IANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPGFK-PFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAYNLPPTSQPGGGGTSGAATSSVATSIAAATGGSNTSGAGPSGLAAAPLPQLPPPHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAP-GESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSVLDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHAKSEKSFENSMDHLITSPASAQAAARIHLFTLLYEDCKVLCARMVDSSKVITSMTTLLDTFQKVI---KGLKDGASTVQIE----TPKWMTPMVLFIDLHEKVILGINRRAKLKDVCTRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPK-PISSEGETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRLILKALPPRAGAPTSMGSLNEVSHSVIADLLNFLVQPEVESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRTQVIIRNSSSGDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEEDLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL--INKSG-KDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAA-----QAIQQMPAFKNQQHTMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQP--GTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEATRAQGDETVEP-DAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILEREGNPEILAEVIEH---SIIEGDDTQHDSQMITQDESQF 2552
MKI+RSKLKKS SEVP DC+ALI++L + + + L EL++IETW YGKCEL+HW++VLDLCD +LE R H E +W + D S Q+ KELLLWTLHFTTLLIEHSFSRHLY+SMEHLT++L+S+ L+V+L VLNLLYMFSKRSNFI+RL +E+R LLSRLTYLA SWGG+ENGFGLA+CC D P+ ++P SATTL+FE+++E+ G+ G R ++ + T IHV V+ + +TPAQ+M+DL + VP QM LFT++RLA +FSDY +RL+CVQARLQALSVLIY N L D SLLY GLLEELV+VLE++ LMEI+AAAL+ LT+IIHLDRNP+FPKLNTIIDVTGAA YHGFLPVLVR+CI SLT ++D+ PFP PLATALFSFLYHLASYEAGGEALV+CGM+ESLL+V+ WP +EL+HITFVTRAVRVIDLITNLDM +FQ H G+ FINRLE EV+ CR++QPYEIR + AA A+ A ++ G+ + E+E+D A S+S A +P T P+PGV+CLPQR+ALLKSMLNFLKKAIQD +FSDSIRHVMDG LP +LKHIISNAEYYG SLFLL TDVVT YVFQEPSLLSSLQD GLTDVVLHALLVKDVPATREVLGSLPNVFSALCLN RGL +FV CKPF+RLFKVLLSPDYLPAMRRRRSSDPMGDTA+NLGNAMDELMRHQPSL+ AT II+LLEELC G +P YI S K E + E +EE+DE N + +E + A +EK PVPLVDY+ NVMKFV+AILSNNSTDDHCREFV Q+GL PLMGILGLPNLP+DFP ACQAVAAV K++LNL EPLVL QGL L VL LQ LH PL+PPGGSVLLREL SS EA T P TPLLH M+AAHAYI MFVHVCRTGQ+DIRT+SI HWGS LGL VL+GLSKLYTSLVWESTVLLALCS++TLP G +FG+AD++KLLP P K+ + S A G +N SV AA++ LST+ A ME+D + + +K + Q+K +KPLLS +SRLGRALAELF LLVKLCVGSP+R RR P TP PSP A+AVA +LT+LL +GL+W+PP TSP P+FRLTF ICSVGFT+PMLFDEKK PYHLML KF++ GGQ AFFD FYWALS G V+P+ GLE+ DLP+GTGEFLD+WL+LLEKMVNPK IL+SPH LP+K G K PF P+KY+ THR AF+AVM LWGK+PLKVYG RM+ESVL+ILCHILKGE II+EK + + P+ GTS AATS +N HL LMDMGFPR++C++A+ T SL+QATDYLL+ P P++ AP G +A + G D+D +MRAIA+SL + + + + K+P E + PL K VLD F AL GCLSL+D+LP+ V++VC L+VA+ NRNG ++S L SL +E+G + L +H S + D +I S + +A+ RIHLFTLL E+ K+ CAR ++ ++ + LL + Q+V+ + DG + TPKWM P++L +DL+EK+ LG RRA ++ V + WK++DI SGKWC Y AANNK IDDAFWAG+ S+++ GRR+Y +QF SM+Q NEETGNRRP+M+ +K++PK P E A + DE+ MD E+ +ES++ S + GL L++ L+++CV L+ IP D + L+A +RLCLRLT+ + HA F LGG+K LLNL+Q S F GF +LATLL+RH++E+P+TL HTMEKV+R T ++ P+ K+ H+L R LAPAACR + F +V K LR DL+L NKRG E+D+RL++KALPPR G ++ L+ V+ ++ DLL+ L P + A +PP +V R Q +IRNSSS DL ++ + K+ S+ E D ++RPLL KSAICRLLAELV+SY GCA+L+ +H++ + SEL+ EET ALA++LD LL +G KD +V+TLVA++ASCN+ EAQ+ LV+EVK AL RAL PESN KH ++Q+LT L+STMIESCPA QA+ AFK Q MNN+VK MLK+G++ DLARI H LD+SSP +A TVNAALKPLE L+RIVN P +A RP EG E +S + N+T S+ TRAQ E DAE TE D+S A ES++ +++ +G E D ++ I+++ + A ++ S +E DD+ HDSQM T +ES+F
Sbjct: 1 MKIDRSKLKKSSSEVPADCKALIERLKSCSE---DELLAELKKIETWTYGKCELYHWVDVLDLCDSILE---RACHKERENSWVLACDLSQNKQL----------------------KELLLWTLHFTTLLIEHSFSRHLYNSMEHLTSMLSSNCLNVVLGVLNLLYMFSKRSNFISRLASERRVSLLSRLTYLAESWGGKENGFGLAECCKDLPLSSYPSSATTLHFEFYAEL---MGDGTG---RQSGSGKSSGSFVTVIHVEHVEWLNKTPAQLMEDL-IDVHHVPLDKQMQLFTHIRLAHAFSDYRRRLQCVQARLQALSVLIYCNALADAAPSLLYAGLLEELVDVLELTEPQLMEIRAAALRTLTAIIHLDRNPNFPKLNTIIDVTGAALYHGFLPVLVRSCIASLTGQHTEQDMIPFPLPLATALFSFLYHLASYEAGGEALVACGMMESLLRVVSWPGSELEHITFVTRAVRVIDLITNLDMHAFQAHGGLQIFINRLESEVDACRKEQPYEIRPPIS-----------------AATASNAGVNIPGDDPTNESESEMEVDTGAASSSSPASQPLTE----PIPGVTCLPQRAALLKSMLNFLKKAIQDSSFSDSIRHVMDGSLPAALKHIISNAEYYGPSLFLLGTDVVTVYVFQEPSLLSSLQDSGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNARGLASFVACKPFQRLFKVLLSPDYLPAMRRRRSSDPMGDTATNLGNAMDELMRHQPSLRTDATAGIIQLLEELCAFGHDPNYIASSRTQKFEVAQNTSARTGPVEATGGGSSDEEEDEEEEATSSSNPAPPRGIETEAGAVVPSEKTPVPLVDYVLNVMKFVDAILSNNSTDDHCREFVSQRGLIPLMGILGLPNLPVDFPVTPACQAVAAVCKSILNLAHEPLVLKQGLDHLQVVLNTLQQLHKPLDPPGGSVLLRELASSSNPTEAITSPNTTPLLHAMAAAHAYIMMFVHVCRTGQSDIRTLSINHWGSPLGLPVLKGLSKLYTSLVWESTVLLALCSDETLPLGCQFGRADLEKLLP-------------------------PELKTVAEGPSCSGAVGMELGTN-SVAAALQALSTEASPPAAMEID-------HDQLDAKDRPRPEKLTPAQQHQMKMVKPLLSTASRLGRALAELFGLLVKLCVGSPIRHRRAHQAPPTPLIPSPSAQAVAGALTQLLTSGLAWEPPVTSPTPKFRLTFLICSVGFTSPMLFDEKKLPYHLMLHKFVSCGGQSAFFDAFYWALSAGGRVSPDQGLEYSDLPEGTGEFLDAWLMLLEKMVNPKNILDSPHQLPNKALHGSKHPFSPLKYLISTHRQAFDAVMRLWGKRPLKVYGARMTESVLTILCHILKGEVIIQEKLALEKVEEPA-------------------------------GTSTAATSDADL-------------------------------VNSQHLQALMDMGFPRDQCVEALLFTTSLEQATDYLLSTP----PSL-----LRAPHGAVAAAAAAAVSTAMDTDGNDEDAVMRAIALSLTAD----------------SDRADDAGVVEKEPQEHVLQG------EPLSKQVLDQFVQQALGGCLSLLDSLPETVYRVCSLVVAMVNRNGEMYRDSMLASLAKEIGSCVTSLQEHLDGVDSLGSKADAVIQSSDAMKASVRIHLFTLLCEEMKIACARQLEEKDLVLKLIRLLSSGQQVLAVRAKVNDGNECKPADGVGTTPKWMAPLLLCLDLYEKMSLGTRRRAAMEKVTSHMWKWFDIVSGKWCPYTAANNKIIDDAFWAGQNSVRVTAGRRRYLLQFSSMIQVNEETGNRRPMMLSLKEKPKEPTVDE---ANRMDEN------AAMDVDETEKESSKG------------SQLSGLNLDQGSELIKACVGLLCIPADPDTLHAAMRLCLRLTRHYQHAEKFASLGGVKQLLNLSQSSTFSGFLTLATLLLRHLVEEPETLHHTMEKVLRAYTGPSAAPSTKEFHFLMRVLAPAACRDVQIFSEVCKTFLRADLSLTNKRGD--EEDNRLLMKALPPRPGC--NLPQLSGVAREIVCDLLDALTIPVPPEETITTPPPATETDGTAPVAIPPARRYAGRNV-----------RQQELIRNSSSNDLLNHEAEETVDDARRDLLARKKD----SSSEEMDRRRRPLLPKSAICRLLAELVRSYAGCARLVAEHIFPAKTSELVSEETSALAFLLDNLLPSCQTAGDKDSPALVRTLVASLASCNHAPEAQSCLVAEVKGALNRALTMPESNDKHNRLQSLTALLSTMIESCPATGLQPPQALTT--AFKQQNLGMNNMVKFMLKRGLVNDLARIPHCLDMSSPLMAFTVNAALKPLEALSRIVNLPPAAPVAGRPATKPKSGDEG--------EQQESSALLQGNST-SDNTRAQAVEISSALDAENTEQDVSAAGESLENLADN------DGGGESGDNNLEDIMDQHSQALVEALIVAAENPSQMETDDSAHDSQMRTHNESEF 2341
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Match: gi|1351495515|ref|XP_023930490.1| (E3 ubiquitin-protein ligase HUWE1 [Lingula anatina]) HSP 1 Score: 2310.41 bits (5986), Expect = 0.000e+0 Identity = 1316/2678 (49.14%), Postives = 1696/2678 (63.33%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPKLNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAA----------AACEDVEG--EMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVP-------GVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEV-PSTTNEGGSSDEEEEEDDETSTNQPQDNT--SQAEQVEQQTPAPQT--EKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNAS-VTAAMENLST-DPDIANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPT-PGFKPFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAYNLPPTSQPGGGGTSGAATSSVATSIAAATGGSNTSGAGPSGLAAAPLPQLPP----PHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSVLDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHAK-----SEKSFENSMDHLITSPASAQAAARIHLFTLLYEDCKVLCARMVDSSKVITSMTTLLDTFQKVIKGLKDGASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVC--TRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPKPISSE-----GETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNN---------KDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRL-------ILKALPPRAGAPTSMGSLNEVSHSVIADLLNFL------VQPEVESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRTQVIIRNSSSGDLQ-------VPATDQAIPSENPTTSKEPK--EDNPASTKAEEDLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELLINKS---GKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPAFKNQQHTMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEAT------RAQGDETVEPDAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILERE----GNPEILAEVI------EHSIIEGDDTQ---------------HDSQMITQDESQFDQNVPNMSQDEEDRESEN 2570
MKI+R+KLKK SE+P +C+ LID+L G R+ + ELQ+++TW YGKCELFHW +VLD+ DEVLE A R E + W D G E K+K+LLL L FT LLIEHSFSRHLY+SMEHLT LL+SSD+ V+L VLNLLY+FSKRSNFI RL A+K+Q L+ RLT+LA SWGG++NGFGLA+CC D P+ ++P +ATTL+FE+++E + E AG + T+TV IH+ VD++ + P+QIM+DLL ++ +VPE ++LLFT++RLA SFS + +RLKCVQARLQA+S+L+YSN + DNV SLLY GL+EELV+++E+ L+EIKAAAL+ LTSIIHLDRNP KLN IIDVTGA+SYHGFLP LVRNCI + + D P+PQP ATALFSFLYHLASYE+GGEALVSCGM+ESLLKVI W +HITFVTRAVRV+DLITNLDM +FQ+H G+T FINRLE EVE CR++QP+ IR + S E + +A + VEG + S + ++ A ST E ++ T +P G C PQR+ALLKSMLNFLKKAI DPAFS+SIRH+MDG LP SLKHIISNAEYYG SLFLLATDVVT YVFQEPSLLSSLQD GLTDVVLHALL+KDVPATREVL SLPNVFSALCLNTRGL +FVECKPF+RLFKVLLSPDYLPAMRRRRSSDP+GDTASNLGNAMDELMRHQPSL+ AT AIIKLLEE+C+MG++ KYIC K K E + P T N+ GSS++E++EDD ST T S E Q AP T E+ +PL+DY+ NVMKFVE+ILSNN+TDDHCREFV QKGL PLMGILGLPNLPIDFP +CQAVA+V K+VL L EP VL QGLLQLN+VL++L+PLH PL PPGGSVLLRELV+S + EA ATPLLH +S+AHAYI MFVHVCR GQ+DIR++S+ HWGSELGL VL+GLS+LYTSLVWESTVLL+LCSED LPPG EFGK DM+KLLP EK T+ E + +S ++ ++ V+AAME L+T + + M++D +P+++ E K K S+ L QIKQIKPLLS SSRLGRALAELF LLVKLCVGSP+R RR P P TPSP ARAVAS+L KLL GLSW+PP +P+P+ RLTF ICSVGFTAP+LFD K+PY+LML KF++ GG A F+ F WALSCG +V+ ++GLE P+LPDGTGEFLD+WL+L+EKMVN K ILESPH LP+K T PGF PF ++Y+ T + AF+AVM LWG+KPLKVYG RMSES+L+ILCHI+KGE +I+EK K+ + A+A+G S G G AAAP+ +PP P +NP HL +LMDMGF RE C DA+ T L+QAT+YLL P P AP + G + G ++D++MRAIAMSLGENV++S G+ ++ E + ++ E+D + D PLEK V+D F D + GCL L+DTLP+ V++VCDLLV V RNG + + + L+ + E+ +++ + + ++ L + P +++ A R+HL TLL E+ K+ CA ++ S ++ + LD Q+ + K TPKW+ ++L IDL+EK+ + RR + V + TWK++D +G+WC+ NNK+IDD++ AGE S++ GRR+Y + F +M+Q NEETGNRRP+M+ + + + + G ++ D+ E+ +T + GL E+ T++++ V +I IPV+ + L+AV+RLCLR+T++ H++ FTQLGG ++LLNLTQ S F GF SLATLL+RHV+EDP L+HTMEKV+RT A + N K++HY+ RAL PAACR PE F +VA++VLR+ L +KR EDD R+ ILK +P + P + V DLL+ L +Q E E + G P T A V VT + R ++R SS DLQ TDQ +TS + K E + AEE+ KKRPLL KSAI RLLAELV+SY GCA+LIT H Y+SG +EL+ E+ LA++LD LL N +D + + L+A++A+CN+ EAQ TLVSEVK+AL RALA PES++KH ++QALT ++STMIESCP I FK QQ +N +VK++LKKG+++DLAR+ H+LDLSSP++A TVN ALKPLE L+RIVN P QR K +A NI G + NT+ E + A+ D EPD + EL+IS M P ES+ + E DEEF +M DQ+++R+ G E+LAE+I + + DD HDSQMITQ+ S D V N +++ ES++
Sbjct: 1 MKIDRTKLKKGNSELPAECRNLIDKLKICG---RDGLVTELQKVKTWTYGKCELFHWADVLDVFDEVLEKACRK---EQDKQWVLPCDME----------------GNE------KEKDLLLSVLQFTALLIEHSFSRHLYNSMEHLTTLLSSSDMTVVLGVLNLLYVFSKRSNFITRLAADKKQALIVRLTHLAESWGGKDNGFGLAECCRDLPMSSYPSTATTLHFEFYAEA---AEEKAGSKKN-------TSTVLNTIHIEGVDQIQKLPSQIMEDLLAKF-NVPEDKKVLLFTHIRLAHSFSRHDRRLKCVQARLQAISILVYSNAIQDNVNSLLYTGLIEELVDIVELKTTQLVEIKAAALRTLTSIIHLDRNP---KLNAIIDVTGASSYHGFLPALVRNCIQHMIDPQLD--PYPQPFATALFSFLYHLASYESGGEALVSCGMMESLLKVINWYGDGQEHITFVTRAVRVVDLITNLDMAAFQSHQGLTAFINRLEHEVEICRREQPFVIRPTRSESISSIEYPHSPMSPGRSATTTPMDTDHVTMTTSASSVEGTEDHSGTTAAAAAGVNQGASTSTGTDNETVSSQKTCELPDYSAAKTGFQCFPQRAALLKSMLNFLKKAIPDPAFSESIRHLMDGSLPRSLKHIISNAEYYGPSLFLLATDVVTVYVFQEPSLLSSLQDNGLTDVVLHALLIKDVPATREVLASLPNVFSALCLNTRGLQSFVECKPFDRLFKVLLSPDYLPAMRRRRSSDPLGDTASNLGNAMDELMRHQPSLRTEATKAIIKLLEEVCSMGQDVKYICQKPAPKAELAAAINLRPPTPNDAGSSEDEDDEDDGASTTSQTKPTEPSGGEGTAQDGSAPSTSQERIAIPLMDYVLNVMKFVESILSNNTTDDHCREFVSQKGLGPLMGILGLPNLPIDFPASPSCQAVASVCKSVLTLSHEPQVLKQGLLQLNEVLQSLEPLHKPLPPPGGSVLLRELVNSNNVLEAIHSASATPLLHAVSSAHAYIMMFVHVCRVGQSDIRSISVSHWGSELGLQVLKGLSQLYTSLVWESTVLLSLCSEDILPPGCEFGKVDMEKLLPKDFK----------------------VEKETKGGEEAGKLTRSSGETGSNGVSAAMETLTTSEAADSPMDID-IVPSTAGG----ETEGKKQKISAKLQTQIKQIKPLLSASSRLGRALAELFGLLVKLCVGSPIRHRRSHQIPPPPTTPSPAARAVASALMKLLGNGLSWEPPPAAPVPKLRLTFLICSVGFTAPLLFDNDKYPYYLMLQKFLSCGGHDALFNAFEWALSCGGSVSLDEGLETPELPDGTGEFLDAWLMLIEKMVNTKHILESPHKLPAKSTQPGFVPFSSVQYLVHTQKAAFQAVMHLWGRKPLKVYGSRMSESILAILCHIIKGEAVIKEKLAKE-------------------------------------------LEESGSEQASASGSST----GVGGTAAAPV--VPPVRQEPELNPQHLQQLMDMGFTREHCADALLHTTGLEQATEYLLTHPVP------------APALQVSGLQDSLGLDVDMVETEEDQMMRAIAMSLGENVIMSTD-----GQDQQKKEEEEKKKKEQEEVEQDKQRKED----PLEKEVIDHFTDTMIPGCLKLLDTLPETVYRVCDLLVVVTQRNGDKWRANMLECIAHEISVCASKILEACQPLTTSDTRTVSEWASGLSSMPEASRLATRLHLLTLLAEEMKMPCAEAMERSNILELLVQTLDAAQQCLSAAK------LTTTPKWLAALLLLIDLYEKMSMVSKRRMAAEAVLGDSHTWKWFDDRTGRWCNCSVNNNKTIDDSYRAGEASVRFTAGRRRYIVHFNTMIQVNEETGNRRPIMLSLPSKEEKEKDKKEEEVGAALGGVEKTGVGKVEEKKDEKMETEETKKTS-----------KFVKGLTPEQINTIVRASVGMIAIPVEPDTLHAVMRLCLRVTREHQHSLTFTQLGGARLLLNLTQASAFQGFTSLATLLLRHVLEDPANLRHTMEKVVRTAAAGTGSSMNGVAQGSIGAKEMHYVLRALGPAACRQPELFKEVAQNVLRIALPPPSKRD---EDDSRITGPNAAQILKTVPVKQSKPVDVAL---AMKQVTKDLLDALAVKYTWLQEENEKEE--------------GGRSPQTIAEAIQEVVT-EVTRELSGRGG-MLRQDSSVDLQQQDDDTTQAQTDQNATEAGQSTSGQDKEAEKDREKKAAEEEKKKRPLLLKSAILRLLAELVRSYSGCAQLITSHTYQSGQTELVSEDCNVLAFLLDNLLPNTQTTGDRDCPALARVLLASLAACNHSPEAQNTLVSEVKAALQRALALPESSVKHARVQALTGMISTMIESCPTPGHIPNQ-VFKGQQSHVNQMVKVLLKKGLVMDLARVPHSLDLSSPHMATTVNTALKPLETLSRIVNSPTVQTQRQAKQKGTGVAALQSAEQVANNIQEGEELSQNTSTVEPSDETLEGAAEEDTQEEPDTQE-ELEIS----DMPPMDESE---IREAQDEEFQDMFDQLIQRDRGGHGENEVLAEMIIEPDEPSQLLTQTDDGDEVLVDVEVDTDDVDPHDSQMITQEIS--DDEVDNTHVEDDHPESDD 2487
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Match: gi|307207086|gb|EFN84895.1| (E3 ubiquitin-protein ligase HUWE1 [Harpegnathos saltator]) HSP 1 Score: 2290.77 bits (5935), Expect = 0.000e+0 Identity = 1291/2584 (49.96%), Postives = 1661/2584 (64.28%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPK-----LNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEVPSTTNEGGSSDE----------------EEEEDDETSTNQPQDNTSQAEQVEQQTPAPQT---------EKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPDIANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPGFK--PFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAYNLPPTSQPGGGGTSGAATSSVATSIAAATGGSNTSGAGPSGLAAAPLPQLPPPHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSV---LDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHAKSEKSFENSMDHLITSPASAQAAARIHLFTLLYE----DCKVLCARMVDSSKVITSMTTLL--------DTFQKVIKGLKD-GASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVCTRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPKPISSEGETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRLILKALPPRAGAPTSMGSLNEVSHSVIADLLNFLVQPE-VESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRT---QVIIRNSSS-------GDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEEDLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL----INKSGKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPAFKNQQH-TMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEATRAQGDETVEPDAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILEREGN 2520
MKI+RS+LKK SEVP +CQ LI++L + A L EL++I+ W +GKCELFHWI+VLDL D +LE AA ARS D E P EA KELLLW LHFTTLLIEHSFSRHLY+SMEHL LL+S D+ V+L VLNLLYMFSKRSNFI RLN++KRQ LLSRL +LA SWGG+ENGFGLA+CC ++P + FP SATTL+FE+++E P S S P + T IH+ AVD++G+TPAQIM+DLL Y +VP+ QM L T++RLA SFSDY +RL+CVQARLQALSVL+YSN L +N SLL+ GLLEELVE+LE+ HL+EI+AAAL+ LTSIIHLDRNPHFPK LN IIDVTGA+SYHGFLPVLVR CIT+LT+ + D QPFP PLATALFSFLYHLASYEAGGEALVSCGM+ESLLKVI WP +EL+HITFVTRAVRVIDLITN+DMQ FQTH G+ FINRL++EV CR++QP+EI +D+ + E+ EE ++ + + E + ME D T + E +Y A G +CLPQR+ALLKSMLNFLKKAIQDPAFSDSIRHVM+G LP+SLKHIISN+EYYG SLFLLATDVVT YVFQEPSLLSSLQD GLTDVVLHALL+K+VPATREVLGSLPNVFSALCLN RGL +FV+ +PFERLFKVLLSP YL AMRRRRS+DP+GDTASNLGNAMDELMRHQPSLK A AIIKLLEELC +G +P+YIC +A SK++ P+ + TT SS + DE ++ + +TS Q E+Q EK P+ LV+YIHNVMKFV+AILSNNSTDDHCREFV QKGL PL+ ILGLPNLP+D+P QA Q+VA+V K++LNL EP VL GL QLN+VL L+PLH+ +E PGGSVLL EL S+P + A T ATPLLH M+AAH Y+ MFVHVCRT Q++IR +S+ HWGS++GL+VL GLS LY SLVWEST+LLALCSED +P G EFGK DMDKL+P + G S + TA + M +N +T AME LSTDP MEVD S +G S + + Q+K IKPLL SSRLGRALAELF LLVKLC+GSP+RQRRGQ PLTPA PS AR VASSL LL GLSWD SPIP+F+LTF ICSVGFT+PMLFDEK+ PYHLML KF+ S GQ +FF TF+WALS G A +GLEHP+LPDGTGEFLD+WL+LLEKMVNPK IL+SPH++ +K + FK PF+PIKY+ H+ AFEAVM++WGKKPLK YG RM+ES+LSIL HIL+GEKII+E+ +K N+ G GGTSG A S G S+ P +NP++L +LMDMGF R CI+A+ T +++QATDYLL +P + P +L G G D+LM AIA+SL P IG + EEF E ++ +D F +AL+ CL L+D +PD V+++CDLLV + RNG + ++S L+ L +E+G +++ L A+ + EN+ L+ + + A RI+L+TL +E + +V CA++V+ ++ + LL T ++ K KD GA+T ++TPKW+ P++L ID EKV + R+ + V TR WK++D+++GKW Y NN+ I+DA+WAGE+S++I RR+Y I F M Q NE+T NRRP+ + K T +ED S + + MD ES P E+ + + GL +++++CV L+ IPVD +AL+A++++CLRLT+D+ +A +F + GG+K+LL +T S+F G +L+TLL+RH +E+P TL+ MEKVIRT T P K++ ++ R ++ A CR PE + +V +++LRVD+++L K + D+RL++KALPP M + EVS VI DLLN L++P + SD G+ P +P ++ S NAT+ T + ++RN+++ D QV Q IP + T D +S+K E + K+P+LTKSAI ++LAE V+SYG A LIT+H Y +G SE++ E+T ALA++LD+LL N S + + + L+AAIASCN+ + QTTLV+EVK+AL R LA PES+ KH ++Q L L+STMI+SCP Q + K Q+ +N IV+IML+KG+I DLA+I H+LDLSSPN+AAT+NAALKPLE L+RI+NQP PG A +++ + P + G G TT SEAT A G+E E DAE TE DIS AES++P SE Q+ EEG++ +++MDQ+LER+G+
Sbjct: 1 MKIDRSRLKKCTSEVPTECQTLINKLRSCSHA---ELLEELKQIDAWTFGKCELFHWIDVLDLSDSILEEAA-----------ARSPDNPWELACD-LPQNREA-------------KELLLWVLHFTTLLIEHSFSRHLYNSMEHLVTLLSSCDMHVVLGVLNLLYMFSKRSNFITRLNSDKRQALLSRLNHLAESWGGKENGFGLAECCKEYPEKPFPTSATTLHFEFYAENPTVSEPSTTGTSLPSTKKVGQANLVTYIHIEAVDKLGKTPAQIMNDLLKVY-NVPQERQMALLTHIRLAHSFSDYRRRLQCVQARLQALSVLVYSNALQENAHSLLHAGLLEELVELLELPHTHLVEIRAAALRTLTSIIHLDRNPHFPKKPGSRLNMIIDVTGASSYHGFLPVLVRTCITTLTSPQSDGQPFPLPLATALFSFLYHLASYEAGGEALVSCGMMESLLKVINWPGSELEHITFVTRAVRVIDLITNIDMQGFQTHGGLASFINRLDMEVNVCRKEQPFEIE-PLLYQDTPQTSRERSVDREEESSTSRRVHETFDEREESSNPMEFSEDENMSSKTDEKSEQQPDYSAAKT-GKTCLPQRAALLKSMLNFLKKAIQDPAFSDSIRHVMEGTLPSSLKHIISNSEYYGPSLFLLATDVVTVYVFQEPSLLSSLQDNGLTDVVLHALLIKEVPATREVLGSLPNVFSALCLNQRGLESFVKRRPFERLFKVLLSPVYLSAMRRRRSADPLGDTASNLGNAMDELMRHQPSLKVDAIQAIIKLLEELCVLGCDPRYICWRAASKSDNS-PSNI--TTGNRQSSGQSVGVGVGDSGGAGGGGGGSSSDEEEEDEEEASTSSHPQREEQPSPSPAQPGPPPQPREKTPIALVEYIHNVMKFVDAILSNNSTDDHCREFVGQKGLVPLLSILGLPNLPVDYPVAQAAQSVASVCKSILNLAHEPAVLKTGLSQLNEVLTLLKPLHSHMESPGGSVLLHELASAPNLETAFTSDTATPLLHAMNAAHGYVVMFVHVCRTSQSEIRNLSMNHWGSDVGLAVLDGLSDLYMSLVWESTLLLALCSEDIIPAGCEFGKEDMDKLMPPELKT--EGESSTWSGTASADMGSN------------------------GMTTAMEALSTDPPPVPMEVDDKPNVS-------TGRASPN------NYQLKYIKPLLGASSRLGRALAELFGLLVKLCMGSPVRQRRGQQMPLTPALPSQAARNVASSLNGLLTRGLSWDKLPPSPIPKFKLTFLICSVGFTSPMLFDEKRHPYHLMLHKFVLSDGQSSFFATFHWALSGGGQFALTEGLEHPNLPDGTGEFLDAWLMLLEKMVNPKAILDSPHVVSAKCSGMFKEPPFEPIKYLTEIHKKAFEAVMLMWGKKPLKNYGARMTESILSILRHILRGEKIIKERTEKKE------------------------ENVEGAVASGSGGTSGIARS----------GTSDRRDE-------------PEADVNPEYLRQLMDMGFSRAHCIEALMHTLTVEQATDYLLTNP----------ATLRRPDAALNDVG-----GQVMDVETDDDLMHAIAVSL-----------DSPDAHKIG--------------------VGIEEFGR-ETTITENIDEFTHSALEQCLHLLDLMPDTVYRICDLLVTITKRNGDEWRDSMLRQLIREIGILVDHLIQIAEGQD--ENAGTRLVECEEANKVAGRIYLYTLFFEGTFQEMRVPCAQIVEECAILDKLVRLLVVSEGPLTATKNRLAKDNKDSGATTSGVKTPKWLAPLLLLIDRLEKVAILTKRKQLMHKVTTRAWKWFDLSTGKWTLYSPMNNRIINDAYWAGESSVRISCSRRRYLITFSCMTQVNEDTDNRRPITMCFK-----------LNTGNNEDGGSGSDSNMDTDES------------PIEEKRNTVVQGLDPSIAPSIVRACVKLLAIPVDRDALHALMKVCLRLTRDYKNAEVFAREGGVKLLLEMTHMSSFTGCINLSTLLIRHTLEEPRTLRLAMEKVIRTRTQINIPPAYKEILFMTRQISSAVCRNPEVYKEVCENILRVDISVLKKD----DVDNRLVVKALPPSHSPALPM--VEEVSVGVIRDLLNALIKPMPISSD--------------DGTITPTSPEKKSSHSTSSSAIGNATSSTSSRRDVLRNNTTTTNDPLDDDDQV---SQIIPLKIYT-------DIGSSSKVEPEEAKKPVLTKSAILKILAEAVRSYGAVASLITEHTYRAGQSEMVAEDTTALAFLLDKLLPMLPENSSDRTCSGMARMLIAAIASCNHSPDVQTTLVTEVKAALTRTLALPESSEKHAQLQLLIGLISTMIDSCPPTSHTQLRASLKAHQYNNVNYIVRIMLRKGIITDLAKIPHSLDLSSPNMAATINAALKPLETLSRIINQP-----MPG---AVNNKFTKPKNRTVQEEPIGEQTG--TTTSEATHAVGEEANE-DAENTEHDISVTAESLEPASEGQVH--EEGDEAALEDIMDQLLERDGS 2349
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Match: gi|749752910|ref|XP_011138822.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X5 [Harpegnathos saltator]) HSP 1 Score: 2290 bits (5933), Expect = 0.000e+0 Identity = 1291/2584 (49.96%), Postives = 1661/2584 (64.28%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPK-----LNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEVPSTTNEGGSSDE----------------EEEEDDETSTNQPQDNTSQAEQVEQQTPAPQT---------EKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPDIANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPGFK--PFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAYNLPPTSQPGGGGTSGAATSSVATSIAAATGGSNTSGAGPSGLAAAPLPQLPPPHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSV---LDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHAKSEKSFENSMDHLITSPASAQAAARIHLFTLLYE----DCKVLCARMVDSSKVITSMTTLL--------DTFQKVIKGLKD-GASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVCTRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPKPISSEGETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRLILKALPPRAGAPTSMGSLNEVSHSVIADLLNFLVQPE-VESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRT---QVIIRNSSS-------GDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEEDLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL----INKSGKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPAFKNQQH-TMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEATRAQGDETVEPDAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILEREGN 2520
MKI+RS+LKK SEVP +CQ LI++L + A L EL++I+ W +GKCELFHWI+VLDL D +LE AA ARS D E P EA KELLLW LHFTTLLIEHSFSRHLY+SMEHL LL+S D+ V+L VLNLLYMFSKRSNFI RLN++KRQ LLSRL +LA SWGG+ENGFGLA+CC ++P + FP SATTL+FE+++E P S S P + T IH+ AVD++G+TPAQIM+DLL Y +VP+ QM L T++RLA SFSDY +RL+CVQARLQALSVL+YSN L +N SLL+ GLLEELVE+LE+ HL+EI+AAAL+ LTSIIHLDRNPHFPK LN IIDVTGA+SYHGFLPVLVR CIT+LT+ + D QPFP PLATALFSFLYHLASYEAGGEALVSCGM+ESLLKVI WP +EL+HITFVTRAVRVIDLITN+DMQ FQTH G+ FINRL++EV CR++QP+EI +D+ + E+ EE ++ + + E + ME D T + E +Y A G +CLPQR+ALLKSMLNFLKKAIQDPAFSDSIRHVM+G LP+SLKHIISN+EYYG SLFLLATDVVT YVFQEPSLLSSLQD GLTDVVLHALL+K+VPATREVLGSLPNVFSALCLN RGL +FV+ +PFERLFKVLLSP YL AMRRRRS+DP+GDTASNLGNAMDELMRHQPSLK A AIIKLLEELC +G +P+YIC +A SK++ P+ + TT SS + DE ++ + +TS Q E+Q EK P+ LV+YIHNVMKFV+AILSNNSTDDHCREFV QKGL PL+ ILGLPNLP+D+P QA Q+VA+V K++LNL EP VL GL QLN+VL L+PLH+ +E PGGSVLL EL S+P + A T ATPLLH M+AAH Y+ MFVHVCRT Q++IR +S+ HWGS++GL+VL GLS LY SLVWEST+LLALCSED +P G EFGK DMDKL+P + G S + TA + M +N +T AME LSTDP MEVD S +G S + + Q+K IKPLL SSRLGRALAELF LLVKLC+GSP+RQRRGQ PLTPA PS AR VASSL LL GLSWD SPIP+F+LTF ICSVGFT+PMLFDEK+ PYHLML KF+ S GQ +FF TF+WALS G A +GLEHP+LPDGTGEFLD+WL+LLEKMVNPK IL+SPH++ +K + FK PF+PIKY+ H+ AFEAVM++WGKKPLK YG RM+ES+LSIL HIL+GEKII+E+ +K N+ G GGTSG A S G S+ P +NP++L +LMDMGF R CI+A+ T +++QATDYLL +P + P +L G G D+LM AIA+SL P IG + EEF E ++ +D F +AL+ CL L+D +PD V+++CDLLV + RNG + ++S L+ L +E+G +++ L A+ + EN+ L+ + + A RI+L+TL +E + +V CA++V+ ++ + LL T ++ K KD GA+T ++TPKW+ P++L ID EKV + R+ + V TR WK++D+++GKW Y NN+ I+DA+WAGE+S++I RR+Y I F M Q NE+T NRRP+ + K T +ED S + + MD ES P E+ + + GL +++++CV L+ IPVD +AL+A++++CLRLT+D+ +A +F + GG+K+LL +T S+F G +L+TLL+RH +E+P TL+ MEKVIRT T P K++ ++ R ++ A CR PE + +V +++LRVD+++L K + D+RL++KALPP M + EVS VI DLLN L++P + SD G+ P +P ++ S NAT+ T + ++RN+++ D QV Q IP + T D +S+K E + K+P+LTKSAI ++LAE V+SYG A LIT+H Y +G SE++ E+T ALA++LD+LL N S + + + L+AAIASCN+ + QTTLV+EVK+AL R LA PES+ KH ++Q L L+STMI+SCP Q + K Q+ +N IV+IML+KG+I DLA+I H+LDLSSPN+AAT+NAALKPLE L+RI+NQP PG A +++ + P + G G TT SEAT A G+E E DAE TE DIS AES++P SE Q+ EEG++ +++MDQ+LER+G+
Sbjct: 1 MKIDRSRLKKCTSEVPTECQTLINKLRSCSHA---ELLEELKQIDAWTFGKCELFHWIDVLDLSDSILEEAA-----------ARSPDNPWELACD-LPQNREA-------------KELLLWVLHFTTLLIEHSFSRHLYNSMEHLVTLLSSCDMHVVLGVLNLLYMFSKRSNFITRLNSDKRQALLSRLNHLAESWGGKENGFGLAECCKEYPEKPFPTSATTLHFEFYAENPTVSEPSTTGTSLPSTKKVGQANLVTYIHIEAVDKLGKTPAQIMNDLLKVY-NVPQERQMALLTHIRLAHSFSDYRRRLQCVQARLQALSVLVYSNALQENAHSLLHAGLLEELVELLELPHTHLVEIRAAALRTLTSIIHLDRNPHFPKKPGSRLNMIIDVTGASSYHGFLPVLVRTCITTLTSPQSDGQPFPLPLATALFSFLYHLASYEAGGEALVSCGMMESLLKVINWPGSELEHITFVTRAVRVIDLITNIDMQGFQTHGGLASFINRLDMEVNVCRKEQPFEIE-PLLYQDTPQTSRERSVDREEESSTSRRVHETFDEREESSNPMEFSEDENMSSKTDEKSEQQPDYSAAKT-GKTCLPQRAALLKSMLNFLKKAIQDPAFSDSIRHVMEGTLPSSLKHIISNSEYYGPSLFLLATDVVTVYVFQEPSLLSSLQDNGLTDVVLHALLIKEVPATREVLGSLPNVFSALCLNQRGLESFVKRRPFERLFKVLLSPVYLSAMRRRRSADPLGDTASNLGNAMDELMRHQPSLKVDAIQAIIKLLEELCVLGCDPRYICWRAASKSDNS-PSNI--TTGNRQSSGQSVGVGVGDSGGAGGGGGGSSSDEEEEDEEEASTSSHPQREEQPSPSPAQPGPPPQPREKTPIALVEYIHNVMKFVDAILSNNSTDDHCREFVGQKGLVPLLSILGLPNLPVDYPVAQAAQSVASVCKSILNLAHEPAVLKTGLSQLNEVLTLLKPLHSHMESPGGSVLLHELASAPNLETAFTSDTATPLLHAMNAAHGYVVMFVHVCRTSQSEIRNLSMNHWGSDVGLAVLDGLSDLYMSLVWESTLLLALCSEDIIPAGCEFGKEDMDKLMPPELKT--EGESSTWSGTASADMGSN------------------------GMTTAMEALSTDPPPVPMEVDDKPNVS-------TGRASPN------NYQLKYIKPLLGASSRLGRALAELFGLLVKLCMGSPVRQRRGQQMPLTPALPSQAARNVASSLNGLLTRGLSWDKLPPSPIPKFKLTFLICSVGFTSPMLFDEKRHPYHLMLHKFVLSDGQSSFFATFHWALSGGGQFALTEGLEHPNLPDGTGEFLDAWLMLLEKMVNPKAILDSPHVVSAKCSGMFKEPPFEPIKYLTEIHKKAFEAVMLMWGKKPLKNYGARMTESILSILRHILRGEKIIKERTEKKE------------------------ENVEGAVASGSGGTSGIARS----------GTSDRRDE-------------PEADVNPEYLRQLMDMGFSRAHCIEALMHTLTVEQATDYLLTNP----------ATLRRPDAALNDVG-----GQVMDVETDDDLMHAIAVSL-----------DSPDAHKIG--------------------VGIEEFGR-ETTITENIDEFTHSALEQCLHLLDLMPDTVYRICDLLVTITKRNGDEWRDSMLRQLIREIGILVDHLIQIAEGQD--ENAGTRLVECEEANKVAGRIYLYTLFFEGTFQEMRVPCAQIVEECAILDKLVRLLVVSEGPLTATKNRLAKDNKDSGATTSGVKTPKWLAPLLLLIDRLEKVAILTKRKQLMHKVTTRAWKWFDLSTGKWTLYSPMNNRIINDAYWAGESSVRISCSRRRYLITFSCMTQVNEDTDNRRPITMCFK-----------LNTGNNEDGGSGSDSNMDTDES------------PIEEKRNTVVQGLDPSIAPSIVRACVKLLAIPVDRDALHALMKVCLRLTRDYKNAEVFAREGGVKLLLEMTHMSSFTGCINLSTLLIRHTLEEPRTLRLAMEKVIRTRTQINIPPAYKEILFMTRQISSAVCRNPEVYKEVCENILRVDISVLKKD----DVDNRLVVKALPPSHSPALPM--VEEVSVGVIRDLLNALIKPMPISSD--------------DGTITPTSPEKKSSHSTSSSAIGNATSSTSSRRDVLRNNTTTTNDPLDDDDQV---SQIIPLKIYT-------DIGSSSKVEPEEAKKPVLTKSAILKILAEAVRSYGAVASLITEHTYRAGQSEMVAEDTTALAFLLDKLLPMLPENSSDRTCSGMARMLIAAIASCNHSPDVQTTLVTEVKAALTRTLALPESSEKHAQLQLLIGLISTMIDSCPPTSHTQLRASLKAHQYNNVNYIVRIMLRKGIITDLAKIPHSLDLSSPNMAATINAALKPLETLSRIINQP-----MPG---AVNNKFTKPKNRTVQEEPIGEQTG--TTTSEATHAVGEEANE-DAENTEHDISVTAESLEPASEGQVH--EEGDEAALEDIMDQLLERDGS 2349
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Match: gi|752884680|ref|XP_011260092.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X4 [Camponotus floridanus]) HSP 1 Score: 2286.92 bits (5925), Expect = 0.000e+0 Identity = 1283/2587 (49.59%), Postives = 1656/2587 (64.01%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPK-----LNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACED-VEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEVPSTTNEGGSSDEEEEEDDETSTNQPQD------------------NTSQAEQVEQQ---------TPAPQTEKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPDIANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPT---PGFKPFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAYNLPPTSQPGGGGTSGAATSSVATSIAAATGGSNTSGAGPSGLAAAPLPQLPPPHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFP-TTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSVLDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHAKSEKSFENSMDHLITSPASAQAAARIHLFTLLYE----DCKVLCARMVDSSKVITSMTTLLDTFQ--------KVIKGLKDGASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVCTRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPKPISSEGETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRLILKALPPRAGAPTSMGSLNEVSHSVIADLLNFLVQP-EVESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNA-------TNRTQVIIRNSSSGDLQVPATDQA---IPSENPTTSKEPKEDNPASTKAEEDLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL----INKSGKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPA--FKNQQHTMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAEN-IDSGTNVGTNTTNSEATRAQGDETVEPDAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILEREGN 2520
MKI+RS+LKK SEVP +CQALI +L + A L EL++I+ W +GKCELFHWI+VLDL D +LE AA A+S D E P EA KELLLW LHFTTLLIEHSFSRHLY+SMEHL LL+S D+ V+L VLNLLYMFSKRSNFI RLN +KRQ LLSRL +LA SWGG+ENGFGLA CC + P + FP SATTL+FE+++E P S P T + T IH+ VD++G+TPAQIM+DLL Y +VP+ QM L T++RLA SFSDY +RL+CVQARLQALSVL+YSN L +N SLL+ GLLEELVE+LE+ HL+EI+AAAL+ LTSIIHLDRNPHFPK LN IIDVTGA+SYHGFLPVLVR CIT+LT+ + D QPFP PLATALFSFLYHLASYEAGGEALVSCGM+ESLLKVI WP +EL+HITFVTRAVRVIDLITN+DMQ FQTH+G+ FINRL++EV CR++QP+EI +D+ + E+ EE ++ + D + E + ME+ D T + +E +Y A G +CLPQR+ALLKSMLNFLKKAIQDPAFSDSIRHVM+G LP+SLKHIISN+EYYG SLFLLATDVVT YVFQEPSLLSSLQD GLTDVVLHALL+K+VPATREVLGSLPNVFSALCLN RGL +FV+ +PFERLFKVLLSP YL AMRRRRS+DP+GDTASNLGNAMDELMRHQPSLK A AIIKLLEELC +G +P+YIC +A SK+E PS T G + + +TS Q E+Q P EK P+ LV+YIHNVMKFV+AILSNNSTDDHCREFV QKGL PL+ ILGLPNLP+D+P QA Q+VA+V K++LNL EPLVL GL QLN+VL L+PLH+ +E PGGSVLL EL S+P + A T ATPLLH M+AAH Y+ MFVHVCRT Q+DIR +S+ HWGSELGL+VL+GLS+LY SLVWEST+LLALCSED +P G EFGK DMDKL+P + G S + T + M +N +T AME LSTDP MEVD +S ++++ ++ Q+K IKPLL SSRLGRAL+ELF LLVKLC+GSP+RQRRGQ PLTP PS AR+VA++L LL GLSWD SPIP+F+LTF ICSVGFT+PMLFDEK+ PYHLML KF+ GQ AFF TF+WALS G + +GLEHP+LPDGTGEFLD+WL+LLEKMVNPK IL+SPH++ SK T + FDPIKY+ H+ AFEAVM++WGKKPLK YG RM+ES+LSIL HIL+GEKII+E+ +K+ +TAS G G SG T S GS+ P +NP++L +LMDMGF R CI+A+ T +++QATDYLL +P P+ SV SG G AD D++M AIAMSLG+ + G +I RET I++ +D F NAL CL+L+D +PD V+++CDLLV + RNG + ++ L+ L +EVG++++ L D A+S+ EN+ L+ + + + R++L+TL +E + +V CA++V+ ++ + LL + K++K KD + + ++TPKW+ P++L ID EKV R+ + V TR WK++D+++GKW Y NN+ I+ A+WAGE+S++I RR+Y I F M Q NE++ NRRP+ + K ++++ E + D N MD ES P E+ + I GL +++++CV L+ IPVD +AL+AVL++CLRLT+D+ +A +F + GG+K+LL +TQ +F G +L TLL+RH +E+P TL+ MEKVIR T + P K++ ++ R ++ A CR PE + +V +++LRVD++ L K + D+RL++KALPP M + EVS VI D+LN L++P V SD GS P +P + S NA ++R V+ N+++ + + DQ IP + T D ++K E + K+P+LTKSAI ++LAE V+SYG A LIT+H Y G SE++ E+T ALA++LD+LL N + ++ + L+AAIASCN+ + QTTLV+EVK+AL R LA PES+ KH ++Q L L+STMI+SCP + Q+ A +Q + +N IV+IML+KG+I DLA+I H+LDLSSPN+A T+NAALKPLE L+RI+NQ P PG + + ++ E I G T TT SEAT A G+E E DAE TE DIS AES++P SESQ+ EEG++ +++MDQ+LER+G+
Sbjct: 1 MKIDRSRLKKCTSEVPAECQALISKLRSCSHA---ELLEELKQIDAWTFGKCELFHWIDVLDLSDSILEEAA-----------AKSPDNPWELACD-LPQNREA-------------KELLLWVLHFTTLLIEHSFSRHLYNSMEHLVTLLSSCDMHVVLGVLNLLYMFSKRSNFITRLNNDKRQALLSRLNHLAESWGGKENGFGLADCCKEFPEKAFPASATTLHFEFYAENPIIMEPSTTGTSLPSTKKVGQTNLVTYIHIENVDKLGKTPAQIMNDLLKVY-NVPQERQMALLTHIRLAHSFSDYRRRLQCVQARLQALSVLVYSNALQENAHSLLHAGLLEELVELLELPHTHLVEIRAAALRTLTSIIHLDRNPHFPKKPGSRLNMIIDVTGASSYHGFLPVLVRTCITTLTSPQPDGQPFPLPLATALFSFLYHLASYEAGGEALVSCGMMESLLKVINWPGSELEHITFVTRAVRVIDLITNIDMQGFQTHSGLASFINRLDMEVNVCRKEQPFEIE-PLLYQDTPQTSRERSVDREEGSSTSRREHDEILDEREESSNLMEISEDENTSSKTDEKIEQQPDYSAAKT-GKTCLPQRAALLKSMLNFLKKAIQDPAFSDSIRHVMEGTLPSSLKHIISNSEYYGPSLFLLATDVVTVYVFQEPSLLSSLQDNGLTDVVLHALLIKEVPATREVLGSLPNVFSALCLNQRGLESFVKRRPFERLFKVLLSPVYLSAMRRRRSADPLGDTASNLGNAMDELMRHQPSLKVDAIAAIIKLLEELCVLGCDPRYICWRAASKSE-----NSPSNTASGNRQSSGQSVGVGVGDSSGGGGGGGGSSSDEEEEDEEEASTSSHPQREEQPTLSPAQPGPPPQPREKTPIALVEYIHNVMKFVDAILSNNSTDDHCREFVAQKGLVPLLSILGLPNLPVDYPVAQAAQSVASVCKSILNLAHEPLVLKTGLSQLNEVLNLLKPLHSHMESPGGSVLLHELASAPNLETAFTSDTATPLLHAMNAAHGYVVMFVHVCRTSQSDIRNLSMNHWGSELGLTVLKGLSELYMSLVWESTLLLALCSEDIIPAGCEFGKEDMDKLMPPELKN--EGESSSWSATVSADMGSN------------------------GMTTAMEALSTDPPPVPMEVDDKPNVAS----------TENRPNNY---QLKYIKPLLGASSRLGRALSELFGLLVKLCMGSPVRQRRGQQMPLTPTLPSQAARSVATALNCLLTRGLSWDKLPPSPIPKFKLTFLICSVGFTSPMLFDEKRHPYHLMLQKFVKLNGQNAFFSTFHWALSGGGQFSLSEGLEHPNLPDGTGEFLDAWLMLLEKMVNPKAILDSPHVVSSKCTGLSKFLETFDPIKYLTDIHKKAFEAVMLMWGKKPLKNYGARMTESILSILRHILRGEKIIKERTEKEESGDGATAS--------------------------GSGASGVGTRS---------SGSDRRDE-------------PEADVNPEYLRQLMDMGFSRAHCIEALLHTLTVEQATDYLLTNPATLRRPDASVSDVSGQ------------GLIMDLELADDDQIMHAIAMSLGDT-----ETRKITGADDITVCRET--TITEN---------------------IDEFTQNALGQCLNLLDLMPDTVYRICDLLVTITKRNGDEWRDDTLRQLMREVGRLVDHLIDIAESQD--ENAGSRLVECEEANKLSGRLYLYTLFFEGSFQEMRVPCAQIVEECAILDKLVRLLVVSEAPLTAHKNKIVKDNKD-SGVLSMKTPKWLAPLLLLIDRLEKVATLTKRKQLMHKVTTRAWKWFDLSTGKWTLYSPMNNRIINHAYWAGESSVRISCSRRRYLITFSCMTQVNEDSDNRRPITMGFK-----LNTDKEDGGSSGSDSN------MDTDES------------PEEEKRNTVIQGLDPSIAPSIVRACVKLLAIPVDRDALHAVLKVCLRLTRDYKNAEVFAREGGVKLLLEMTQMCSFTGCVNLCTLLIRHTLEEPRTLRLAMEKVIRARTQSNIPPAYKEILFMTRQISSAVCRNPEVYREVCENILRVDISCLKKD----DIDNRLVVKALPPSHSPALPM--VEEVSVGVIRDMLNALIKPIPVSSD--------------DGSATPTSPEKKTSHTATTSAIGNASSSTSSSSSRRDVLRNNATTTNDPLDDDDQVSQIIPLKIYT-------DIGNNSKVEPEEAKKPILTKSAILKILAEAVRSYGAVASLITEHTYRGGQSEMVSEDTTALAFLLDKLLPMSPENSGDRTCSSMARMLIAAIASCNHSPDVQTTLVTEVKAALTRTLALPESSEKHAQLQLLIGLISTMIDSCPPNMSHTQLRASLRAHQYNNVNYIVRIMLRKGIITDLAKIPHSLDLSSPNMAGTINAALKPLETLSRIINQ-------PMPGAVNNKFTKPKNRTVQEEPIGEGQ---TGTTTSEATHAVGEEANE-DAENTEHDISVTAESLEPTSESQVH--EEGDEAALEDIMDQLLERDGS 2358
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Match: gi|749752914|ref|XP_011138824.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X7 [Harpegnathos saltator]) HSP 1 Score: 2286.15 bits (5923), Expect = 0.000e+0 Identity = 1289/2580 (49.96%), Postives = 1657/2580 (64.22%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPK-----LNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEVPSTTNEGGSSDE----------------EEEEDDETSTNQPQDNTSQAEQVEQQTPAPQT---------EKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPDIANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPGFK--PFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAYNLPPTSQPGGGGTSGAATSSVATSIAAATGGSNTSGAGPSGLAAAPLPQLPPPHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSV---LDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHAKSEKSFENSMDHLITSPASAQAAARIHLFTLLYE----DCKVLCARMVDSSKVITSMTTLL--------DTFQKVIKGLKD-GASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVCTRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPKPISSEGETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRLILKALPPRAGAPTSMGSLNEVSHSVIADLLNFLVQPE-VESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRT---QVIIRNSSS-------GDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEEDLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL----INKSGKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPAFKNQQH-TMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEATRAQGDETVEPDAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILE 2516
MKI+RS+LKK SEVP +CQ LI++L + A L EL++I+ W +GKCELFHWI+VLDL D +LE AA ARS D E P EA KELLLW LHFTTLLIEHSFSRHLY+SMEHL LL+S D+ V+L VLNLLYMFSKRSNFI RLN++KRQ LLSRL +LA SWGG+ENGFGLA+CC ++P + FP SATTL+FE+++E P S S P + T IH+ AVD++G+TPAQIM+DLL Y +VP+ QM L T++RLA SFSDY +RL+CVQARLQALSVL+YSN L +N SLL+ GLLEELVE+LE+ HL+EI+AAAL+ LTSIIHLDRNPHFPK LN IIDVTGA+SYHGFLPVLVR CIT+LT+ + D QPFP PLATALFSFLYHLASYEAGGEALVSCGM+ESLLKVI WP +EL+HITFVTRAVRVIDLITN+DMQ FQTH G+ FINRL++EV CR++QP+EI +D+ + E+ EE ++ + + E + ME D T + E +Y A G +CLPQR+ALLKSMLNFLKKAIQDPAFSDSIRHVM+G LP+SLKHIISN+EYYG SLFLLATDVVT YVFQEPSLLSSLQD GLTDVVLHALL+K+VPATREVLGSLPNVFSALCLN RGL +FV+ +PFERLFKVLLSP YL AMRRRRS+DP+GDTASNLGNAMDELMRHQPSLK A AIIKLLEELC +G +P+YIC +A SK++ P+ + TT SS + DE ++ + +TS Q E+Q EK P+ LV+YIHNVMKFV+AILSNNSTDDHCREFV QKGL PL+ ILGLPNLP+D+P QA Q+VA+V K++LNL EP VL GL QLN+VL L+PLH+ +E PGGSVLL EL S+P + A T ATPLLH M+AAH Y+ MFVHVCRT Q++IR +S+ HWGS++GL+VL GLS LY SLVWEST+LLALCSED +P G EFGK DMDKL+P + G S + TA + M +N +T AME LSTDP MEVD S +G S + + Q+K IKPLL SSRLGRALAELF LLVKLC+GSP+RQRRGQ PLTPA PS AR VASSL LL GLSWD SPIP+F+LTF ICSVGFT+PMLFDEK+ PYHLML KF+ S GQ +FF TF+WALS G A +GLEHP+LPDGTGEFLD+WL+LLEKMVNPK IL+SPH++ +K + FK PF+PIKY+ H+ AFEAVM++WGKKPLK YG RM+ES+LSIL HIL+GEKII+E+ +K N+ G GGTSG A S G S+ P +NP++L +LMDMGF R CI+A+ T +++QATDYLL +P + P +L G G D+LM AIA+SL P IG + EEF E ++ +D F +AL+ CL L+D +PD V+++CDLLV + RNG + ++S L+ L +E+G +++ L A+ + EN+ L+ + + A RI+L+TL +E + +V CA++V+ ++ + LL T ++ K KD GA+T ++TPKW+ P++L ID EKV + R+ + V TR WK++D+++GKW Y NN+ I+DA+WAGE+S++I RR+Y I F M Q NE+T NRRP+ + K T +ED S + + MD ES P E+ + + GL +++++CV L+ IPVD +AL+A++++CLRLT+D+ +A +F + GG+K+LL +T S+F G +L+TLL+RH +E+P TL+ MEKVIRT T P K++ ++ R ++ A CR PE + +V +++LRVD+++L K + D+RL++KALPP M + EVS VI DLLN L++P + SD G+ P +P ++ S NAT+ T + ++RN+++ D QV Q IP + T D +S+K E + K+P+LTKSAI ++LAE V+SYG A LIT+H Y +G SE++ E+T ALA++LD+LL N S + + + L+AAIASCN+ + QTTLV+EVK+AL R LA PES+ KH ++Q L L+STMI+SCP Q + K Q+ +N IV+IML+KG+I DLA+I H+LDLSSPN+AAT+NAALKPLE L+RI+NQP PG A +++ + P + G G TT SEAT A G+E E DAE TE DIS AES++P SE Q+ EEG++ +++MDQ+LE
Sbjct: 1 MKIDRSRLKKCTSEVPTECQTLINKLRSCSHA---ELLEELKQIDAWTFGKCELFHWIDVLDLSDSILEEAA-----------ARSPDNPWELACD-LPQNREA-------------KELLLWVLHFTTLLIEHSFSRHLYNSMEHLVTLLSSCDMHVVLGVLNLLYMFSKRSNFITRLNSDKRQALLSRLNHLAESWGGKENGFGLAECCKEYPEKPFPTSATTLHFEFYAENPTVSEPSTTGTSLPSTKKVGQANLVTYIHIEAVDKLGKTPAQIMNDLLKVY-NVPQERQMALLTHIRLAHSFSDYRRRLQCVQARLQALSVLVYSNALQENAHSLLHAGLLEELVELLELPHTHLVEIRAAALRTLTSIIHLDRNPHFPKKPGSRLNMIIDVTGASSYHGFLPVLVRTCITTLTSPQSDGQPFPLPLATALFSFLYHLASYEAGGEALVSCGMMESLLKVINWPGSELEHITFVTRAVRVIDLITNIDMQGFQTHGGLASFINRLDMEVNVCRKEQPFEIE-PLLYQDTPQTSRERSVDREEESSTSRRVHETFDEREESSNPMEFSEDENMSSKTDEKSEQQPDYSAAKT-GKTCLPQRAALLKSMLNFLKKAIQDPAFSDSIRHVMEGTLPSSLKHIISNSEYYGPSLFLLATDVVTVYVFQEPSLLSSLQDNGLTDVVLHALLIKEVPATREVLGSLPNVFSALCLNQRGLESFVKRRPFERLFKVLLSPVYLSAMRRRRSADPLGDTASNLGNAMDELMRHQPSLKVDAIQAIIKLLEELCVLGCDPRYICWRAASKSDNS-PSNI--TTGNRQSSGQSVGVGVGDSGGAGGGGGGSSSDEEEEDEEEASTSSHPQREEQPSPSPAQPGPPPQPREKTPIALVEYIHNVMKFVDAILSNNSTDDHCREFVGQKGLVPLLSILGLPNLPVDYPVAQAAQSVASVCKSILNLAHEPAVLKTGLSQLNEVLTLLKPLHSHMESPGGSVLLHELASAPNLETAFTSDTATPLLHAMNAAHGYVVMFVHVCRTSQSEIRNLSMNHWGSDVGLAVLDGLSDLYMSLVWESTLLLALCSEDIIPAGCEFGKEDMDKLMPPELKT--EGESSTWSGTASADMGSN------------------------GMTTAMEALSTDPPPVPMEVDDKPNVS-------TGRASPN------NYQLKYIKPLLGASSRLGRALAELFGLLVKLCMGSPVRQRRGQQMPLTPALPSQAARNVASSLNGLLTRGLSWDKLPPSPIPKFKLTFLICSVGFTSPMLFDEKRHPYHLMLHKFVLSDGQSSFFATFHWALSGGGQFALTEGLEHPNLPDGTGEFLDAWLMLLEKMVNPKAILDSPHVVSAKCSGMFKEPPFEPIKYLTEIHKKAFEAVMLMWGKKPLKNYGARMTESILSILRHILRGEKIIKERTEKKE------------------------ENVEGAVASGSGGTSGIARS----------GTSDRRDE-------------PEADVNPEYLRQLMDMGFSRAHCIEALMHTLTVEQATDYLLTNP----------ATLRRPDAALNDVG-----GQVMDVETDDDLMHAIAVSL-----------DSPDAHKIG--------------------VGIEEFGR-ETTITENIDEFTHSALEQCLHLLDLMPDTVYRICDLLVTITKRNGDEWRDSMLRQLIREIGILVDHLIQIAEGQD--ENAGTRLVECEEANKVAGRIYLYTLFFEGTFQEMRVPCAQIVEECAILDKLVRLLVVSEGPLTATKNRLAKDNKDSGATTSGVKTPKWLAPLLLLIDRLEKVAILTKRKQLMHKVTTRAWKWFDLSTGKWTLYSPMNNRIINDAYWAGESSVRISCSRRRYLITFSCMTQVNEDTDNRRPITMCFK-----------LNTGNNEDGGSGSDSNMDTDES------------PIEEKRNTVVQGLDPSIAPSIVRACVKLLAIPVDRDALHALMKVCLRLTRDYKNAEVFAREGGVKLLLEMTHMSSFTGCINLSTLLIRHTLEEPRTLRLAMEKVIRTRTQINIPPAYKEILFMTRQISSAVCRNPEVYKEVCENILRVDISVLKKD----DVDNRLVVKALPPSHSPALPM--VEEVSVGVIRDLLNALIKPMPISSD--------------DGTITPTSPEKKSSHSTSSSAIGNATSSTSSRRDVLRNNTTTTNDPLDDDDQV---SQIIPLKIYT-------DIGSSSKVEPEEAKKPVLTKSAILKILAEAVRSYGAVASLITEHTYRAGQSEMVAEDTTALAFLLDKLLPMLPENSSDRTCSGMARMLIAAIASCNHSPDVQTTLVTEVKAALTRTLALPESSEKHAQLQLLIGLISTMIDSCPPTSHTQLRASLKAHQYNNVNYIVRIMLRKGIITDLAKIPHSLDLSSPNMAATINAALKPLETLSRIINQP-----MPG---AVNNKFTKPKNRTVQEEPIGEQTG--TTTSEATHAVGEEANE-DAENTEHDISVTAESLEPASEGQVH--EEGDEAALEDIMDQLLE 2345
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Match: gi|749752912|ref|XP_011138823.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X6 [Harpegnathos saltator]) HSP 1 Score: 2285.76 bits (5922), Expect = 0.000e+0 Identity = 1289/2580 (49.96%), Postives = 1657/2580 (64.22%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPK-----LNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEVPSTTNEGGSSDE----------------EEEEDDETSTNQPQDNTSQAEQVEQQTPAPQT---------EKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPDIANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPGFK--PFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAYNLPPTSQPGGGGTSGAATSSVATSIAAATGGSNTSGAGPSGLAAAPLPQLPPPHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSV---LDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHAKSEKSFENSMDHLITSPASAQAAARIHLFTLLYE----DCKVLCARMVDSSKVITSMTTLL--------DTFQKVIKGLKD-GASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVCTRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPKPISSEGETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRLILKALPPRAGAPTSMGSLNEVSHSVIADLLNFLVQPE-VESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRT---QVIIRNSSS-------GDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEEDLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL----INKSGKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPAFKNQQH-TMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEATRAQGDETVEPDAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILE 2516
MKI+RS+LKK SEVP +CQ LI++L + A L EL++I+ W +GKCELFHWI+VLDL D +LE AA ARS D E P EA KELLLW LHFTTLLIEHSFSRHLY+SMEHL LL+S D+ V+L VLNLLYMFSKRSNFI RLN++KRQ LLSRL +LA SWGG+ENGFGLA+CC ++P + FP SATTL+FE+++E P S S P + T IH+ AVD++G+TPAQIM+DLL Y +VP+ QM L T++RLA SFSDY +RL+CVQARLQALSVL+YSN L +N SLL+ GLLEELVE+LE+ HL+EI+AAAL+ LTSIIHLDRNPHFPK LN IIDVTGA+SYHGFLPVLVR CIT+LT+ + D QPFP PLATALFSFLYHLASYEAGGEALVSCGM+ESLLKVI WP +EL+HITFVTRAVRVIDLITN+DMQ FQTH G+ FINRL++EV CR++QP+EI +D+ + E+ EE ++ + + E + ME D T + E +Y A G +CLPQR+ALLKSMLNFLKKAIQDPAFSDSIRHVM+G LP+SLKHIISN+EYYG SLFLLATDVVT YVFQEPSLLSSLQD GLTDVVLHALL+K+VPATREVLGSLPNVFSALCLN RGL +FV+ +PFERLFKVLLSP YL AMRRRRS+DP+GDTASNLGNAMDELMRHQPSLK A AIIKLLEELC +G +P+YIC +A SK++ P+ + TT SS + DE ++ + +TS Q E+Q EK P+ LV+YIHNVMKFV+AILSNNSTDDHCREFV QKGL PL+ ILGLPNLP+D+P QA Q+VA+V K++LNL EP VL GL QLN+VL L+PLH+ +E PGGSVLL EL S+P + A T ATPLLH M+AAH Y+ MFVHVCRT Q++IR +S+ HWGS++GL+VL GLS LY SLVWEST+LLALCSED +P G EFGK DMDKL+P + G S + TA + M +N +T AME LSTDP MEVD S +G S + + Q+K IKPLL SSRLGRALAELF LLVKLC+GSP+RQRRGQ PLTPA PS AR VASSL LL GLSWD SPIP+F+LTF ICSVGFT+PMLFDEK+ PYHLML KF+ S GQ +FF TF+WALS G A +GLEHP+LPDGTGEFLD+WL+LLEKMVNPK IL+SPH++ +K + FK PF+PIKY+ H+ AFEAVM++WGKKPLK YG RM+ES+LSIL HIL+GEKII+E+ +K N+ G GGTSG A S G S+ P +NP++L +LMDMGF R CI+A+ T +++QATDYLL +P + P +L G G D+LM AIA+SL P IG + EEF E ++ +D F +AL+ CL L+D +PD V+++CDLLV + RNG + ++S L+ L +E+G +++ L A+ + EN+ L+ + + A RI+L+TL +E + +V CA++V+ ++ + LL T ++ K KD GA+T ++TPKW+ P++L ID EKV + R+ + V TR WK++D+++GKW Y NN+ I+DA+WAGE+S++I RR+Y I F M Q NE+T NRRP+ + K T +ED S + + MD ES P E+ + + GL +++++CV L+ IPVD +AL+A++++CLRLT+D+ +A +F + GG+K+LL +T S+F G +L+TLL+RH +E+P TL+ MEKVIRT T P K++ ++ R ++ A CR PE + +V +++LRVD+++L K + D+RL++KALPP M + EVS VI DLLN L++P + SD G+ P +P ++ S NAT+ T + ++RN+++ D QV Q IP + T D +S+K E + K+P+LTKSAI ++LAE V+SYG A LIT+H Y +G SE++ E+T ALA++LD+LL N S + + + L+AAIASCN+ + QTTLV+EVK+AL R LA PES+ KH ++Q L L+STMI+SCP Q + K Q+ +N IV+IML+KG+I DLA+I H+LDLSSPN+AAT+NAALKPLE L+RI+NQP PG A +++ + P + G G TT SEAT A G+E E DAE TE DIS AES++P SE Q+ EEG++ +++MDQ+LE
Sbjct: 1 MKIDRSRLKKCTSEVPTECQTLINKLRSCSHA---ELLEELKQIDAWTFGKCELFHWIDVLDLSDSILEEAA-----------ARSPDNPWELACD-LPQNREA-------------KELLLWVLHFTTLLIEHSFSRHLYNSMEHLVTLLSSCDMHVVLGVLNLLYMFSKRSNFITRLNSDKRQALLSRLNHLAESWGGKENGFGLAECCKEYPEKPFPTSATTLHFEFYAENPTVSEPSTTGTSLPSTKKVGQANLVTYIHIEAVDKLGKTPAQIMNDLLKVY-NVPQERQMALLTHIRLAHSFSDYRRRLQCVQARLQALSVLVYSNALQENAHSLLHAGLLEELVELLELPHTHLVEIRAAALRTLTSIIHLDRNPHFPKKPGSRLNMIIDVTGASSYHGFLPVLVRTCITTLTSPQSDGQPFPLPLATALFSFLYHLASYEAGGEALVSCGMMESLLKVINWPGSELEHITFVTRAVRVIDLITNIDMQGFQTHGGLASFINRLDMEVNVCRKEQPFEIE-PLLYQDTPQTSRERSVDREEESSTSRRVHETFDEREESSNPMEFSEDENMSSKTDEKSEQQPDYSAAKT-GKTCLPQRAALLKSMLNFLKKAIQDPAFSDSIRHVMEGTLPSSLKHIISNSEYYGPSLFLLATDVVTVYVFQEPSLLSSLQDNGLTDVVLHALLIKEVPATREVLGSLPNVFSALCLNQRGLESFVKRRPFERLFKVLLSPVYLSAMRRRRSADPLGDTASNLGNAMDELMRHQPSLKVDAIQAIIKLLEELCVLGCDPRYICWRAASKSDNS-PSNI--TTGNRQSSGQSVGVGVGDSGGAGGGGGGSSSDEEEEDEEEASTSSHPQREEQPSPSPAQPGPPPQPREKTPIALVEYIHNVMKFVDAILSNNSTDDHCREFVGQKGLVPLLSILGLPNLPVDYPVAQAAQSVASVCKSILNLAHEPAVLKTGLSQLNEVLTLLKPLHSHMESPGGSVLLHELASAPNLETAFTSDTATPLLHAMNAAHGYVVMFVHVCRTSQSEIRNLSMNHWGSDVGLAVLDGLSDLYMSLVWESTLLLALCSEDIIPAGCEFGKEDMDKLMPPELKT--EGESSTWSGTASADMGSN------------------------GMTTAMEALSTDPPPVPMEVDDKPNVS-------TGRASPN------NYQLKYIKPLLGASSRLGRALAELFGLLVKLCMGSPVRQRRGQQMPLTPALPSQAARNVASSLNGLLTRGLSWDKLPPSPIPKFKLTFLICSVGFTSPMLFDEKRHPYHLMLHKFVLSDGQSSFFATFHWALSGGGQFALTEGLEHPNLPDGTGEFLDAWLMLLEKMVNPKAILDSPHVVSAKCSGMFKEPPFEPIKYLTEIHKKAFEAVMLMWGKKPLKNYGARMTESILSILRHILRGEKIIKERTEKKE------------------------ENVEGAVASGSGGTSGIARS----------GTSDRRDE-------------PEADVNPEYLRQLMDMGFSRAHCIEALMHTLTVEQATDYLLTNP----------ATLRRPDAALNDVG-----GQVMDVETDDDLMHAIAVSL-----------DSPDAHKIG--------------------VGIEEFGR-ETTITENIDEFTHSALEQCLHLLDLMPDTVYRICDLLVTITKRNGDEWRDSMLRQLIREIGILVDHLIQIAEGQD--ENAGTRLVECEEANKVAGRIYLYTLFFEGTFQEMRVPCAQIVEECAILDKLVRLLVVSEGPLTATKNRLAKDNKDSGATTSGVKTPKWLAPLLLLIDRLEKVAILTKRKQLMHKVTTRAWKWFDLSTGKWTLYSPMNNRIINDAYWAGESSVRISCSRRRYLITFSCMTQVNEDTDNRRPITMCFK-----------LNTGNNEDGGSGSDSNMDTDES------------PIEEKRNTVVQGLDPSIAPSIVRACVKLLAIPVDRDALHALMKVCLRLTRDYKNAEVFAREGGVKLLLEMTHMSSFTGCINLSTLLIRHTLEEPRTLRLAMEKVIRTRTQINIPPAYKEILFMTRQISSAVCRNPEVYKEVCENILRVDISVLKKD----DVDNRLVVKALPPSHSPALPM--VEEVSVGVIRDLLNALIKPMPISSD--------------DGTITPTSPEKKSSHSTSSSAIGNATSSTSSRRDVLRNNTTTTNDPLDDDDQV---SQIIPLKIYT-------DIGSSSKVEPEEAKKPVLTKSAILKILAEAVRSYGAVASLITEHTYRAGQSEMVAEDTTALAFLLDKLLPMLPENSSDRTCSGMARMLIAAIASCNHSPDVQTTLVTEVKAALTRTLALPESSEKHAQLQLLIGLISTMIDSCPPTSHTQLRASLKAHQYNNVNYIVRIMLRKGIITDLAKIPHSLDLSSPNMAATINAALKPLETLSRIINQP-----MPG---AVNNKFTKPKNRTVQEEPIGEQTG--TTTSEATHAVGEEANE-DAENTEHDISVTAESLEPASEGQVH--EEGDEAALEDIMDQLLE 2345
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Match: gi|749752908|ref|XP_011138821.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X4 [Harpegnathos saltator]) HSP 1 Score: 2285.76 bits (5922), Expect = 0.000e+0 Identity = 1289/2580 (49.96%), Postives = 1657/2580 (64.22%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPK-----LNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEVPSTTNEGGSSDE----------------EEEEDDETSTNQPQDNTSQAEQVEQQTPAPQT---------EKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPDIANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPGFK--PFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAYNLPPTSQPGGGGTSGAATSSVATSIAAATGGSNTSGAGPSGLAAAPLPQLPPPHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSV---LDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHAKSEKSFENSMDHLITSPASAQAAARIHLFTLLYE----DCKVLCARMVDSSKVITSMTTLL--------DTFQKVIKGLKD-GASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVCTRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPKPISSEGETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRLILKALPPRAGAPTSMGSLNEVSHSVIADLLNFLVQPE-VESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRT---QVIIRNSSS-------GDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEEDLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL----INKSGKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPAFKNQQH-TMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEATRAQGDETVEPDAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILE 2516
MKI+RS+LKK SEVP +CQ LI++L + A L EL++I+ W +GKCELFHWI+VLDL D +LE AA ARS D E P EA KELLLW LHFTTLLIEHSFSRHLY+SMEHL LL+S D+ V+L VLNLLYMFSKRSNFI RLN++KRQ LLSRL +LA SWGG+ENGFGLA+CC ++P + FP SATTL+FE+++E P S S P + T IH+ AVD++G+TPAQIM+DLL Y +VP+ QM L T++RLA SFSDY +RL+CVQARLQALSVL+YSN L +N SLL+ GLLEELVE+LE+ HL+EI+AAAL+ LTSIIHLDRNPHFPK LN IIDVTGA+SYHGFLPVLVR CIT+LT+ + D QPFP PLATALFSFLYHLASYEAGGEALVSCGM+ESLLKVI WP +EL+HITFVTRAVRVIDLITN+DMQ FQTH G+ FINRL++EV CR++QP+EI +D+ + E+ EE ++ + + E + ME D T + E +Y A G +CLPQR+ALLKSMLNFLKKAIQDPAFSDSIRHVM+G LP+SLKHIISN+EYYG SLFLLATDVVT YVFQEPSLLSSLQD GLTDVVLHALL+K+VPATREVLGSLPNVFSALCLN RGL +FV+ +PFERLFKVLLSP YL AMRRRRS+DP+GDTASNLGNAMDELMRHQPSLK A AIIKLLEELC +G +P+YIC +A SK++ P+ + TT SS + DE ++ + +TS Q E+Q EK P+ LV+YIHNVMKFV+AILSNNSTDDHCREFV QKGL PL+ ILGLPNLP+D+P QA Q+VA+V K++LNL EP VL GL QLN+VL L+PLH+ +E PGGSVLL EL S+P + A T ATPLLH M+AAH Y+ MFVHVCRT Q++IR +S+ HWGS++GL+VL GLS LY SLVWEST+LLALCSED +P G EFGK DMDKL+P + G S + TA + M +N +T AME LSTDP MEVD S +G S + + Q+K IKPLL SSRLGRALAELF LLVKLC+GSP+RQRRGQ PLTPA PS AR VASSL LL GLSWD SPIP+F+LTF ICSVGFT+PMLFDEK+ PYHLML KF+ S GQ +FF TF+WALS G A +GLEHP+LPDGTGEFLD+WL+LLEKMVNPK IL+SPH++ +K + FK PF+PIKY+ H+ AFEAVM++WGKKPLK YG RM+ES+LSIL HIL+GEKII+E+ +K N+ G GGTSG A S G S+ P +NP++L +LMDMGF R CI+A+ T +++QATDYLL +P + P +L G G D+LM AIA+SL P IG + EEF E ++ +D F +AL+ CL L+D +PD V+++CDLLV + RNG + ++S L+ L +E+G +++ L A+ + EN+ L+ + + A RI+L+TL +E + +V CA++V+ ++ + LL T ++ K KD GA+T ++TPKW+ P++L ID EKV + R+ + V TR WK++D+++GKW Y NN+ I+DA+WAGE+S++I RR+Y I F M Q NE+T NRRP+ + K T +ED S + + MD ES P E+ + + GL +++++CV L+ IPVD +AL+A++++CLRLT+D+ +A +F + GG+K+LL +T S+F G +L+TLL+RH +E+P TL+ MEKVIRT T P K++ ++ R ++ A CR PE + +V +++LRVD+++L K + D+RL++KALPP M + EVS VI DLLN L++P + SD G+ P +P ++ S NAT+ T + ++RN+++ D QV Q IP + T D +S+K E + K+P+LTKSAI ++LAE V+SYG A LIT+H Y +G SE++ E+T ALA++LD+LL N S + + + L+AAIASCN+ + QTTLV+EVK+AL R LA PES+ KH ++Q L L+STMI+SCP Q + K Q+ +N IV+IML+KG+I DLA+I H+LDLSSPN+AAT+NAALKPLE L+RI+NQP PG A +++ + P + G G TT SEAT A G+E E DAE TE DIS AES++P SE Q+ EEG++ +++MDQ+LE
Sbjct: 1 MKIDRSRLKKCTSEVPTECQTLINKLRSCSHA---ELLEELKQIDAWTFGKCELFHWIDVLDLSDSILEEAA-----------ARSPDNPWELACD-LPQNREA-------------KELLLWVLHFTTLLIEHSFSRHLYNSMEHLVTLLSSCDMHVVLGVLNLLYMFSKRSNFITRLNSDKRQALLSRLNHLAESWGGKENGFGLAECCKEYPEKPFPTSATTLHFEFYAENPTVSEPSTTGTSLPSTKKVGQANLVTYIHIEAVDKLGKTPAQIMNDLLKVY-NVPQERQMALLTHIRLAHSFSDYRRRLQCVQARLQALSVLVYSNALQENAHSLLHAGLLEELVELLELPHTHLVEIRAAALRTLTSIIHLDRNPHFPKKPGSRLNMIIDVTGASSYHGFLPVLVRTCITTLTSPQSDGQPFPLPLATALFSFLYHLASYEAGGEALVSCGMMESLLKVINWPGSELEHITFVTRAVRVIDLITNIDMQGFQTHGGLASFINRLDMEVNVCRKEQPFEIE-PLLYQDTPQTSRERSVDREEESSTSRRVHETFDEREESSNPMEFSEDENMSSKTDEKSEQQPDYSAAKT-GKTCLPQRAALLKSMLNFLKKAIQDPAFSDSIRHVMEGTLPSSLKHIISNSEYYGPSLFLLATDVVTVYVFQEPSLLSSLQDNGLTDVVLHALLIKEVPATREVLGSLPNVFSALCLNQRGLESFVKRRPFERLFKVLLSPVYLSAMRRRRSADPLGDTASNLGNAMDELMRHQPSLKVDAIQAIIKLLEELCVLGCDPRYICWRAASKSDNS-PSNI--TTGNRQSSGQSVGVGVGDSGGAGGGGGGSSSDEEEEDEEEASTSSHPQREEQPSPSPAQPGPPPQPREKTPIALVEYIHNVMKFVDAILSNNSTDDHCREFVGQKGLVPLLSILGLPNLPVDYPVAQAAQSVASVCKSILNLAHEPAVLKTGLSQLNEVLTLLKPLHSHMESPGGSVLLHELASAPNLETAFTSDTATPLLHAMNAAHGYVVMFVHVCRTSQSEIRNLSMNHWGSDVGLAVLDGLSDLYMSLVWESTLLLALCSEDIIPAGCEFGKEDMDKLMPPELKT--EGESSTWSGTASADMGSN------------------------GMTTAMEALSTDPPPVPMEVDDKPNVS-------TGRASPN------NYQLKYIKPLLGASSRLGRALAELFGLLVKLCMGSPVRQRRGQQMPLTPALPSQAARNVASSLNGLLTRGLSWDKLPPSPIPKFKLTFLICSVGFTSPMLFDEKRHPYHLMLHKFVLSDGQSSFFATFHWALSGGGQFALTEGLEHPNLPDGTGEFLDAWLMLLEKMVNPKAILDSPHVVSAKCSGMFKEPPFEPIKYLTEIHKKAFEAVMLMWGKKPLKNYGARMTESILSILRHILRGEKIIKERTEKKE------------------------ENVEGAVASGSGGTSGIARS----------GTSDRRDE-------------PEADVNPEYLRQLMDMGFSRAHCIEALMHTLTVEQATDYLLTNP----------ATLRRPDAALNDVG-----GQVMDVETDDDLMHAIAVSL-----------DSPDAHKIG--------------------VGIEEFGR-ETTITENIDEFTHSALEQCLHLLDLMPDTVYRICDLLVTITKRNGDEWRDSMLRQLIREIGILVDHLIQIAEGQD--ENAGTRLVECEEANKVAGRIYLYTLFFEGTFQEMRVPCAQIVEECAILDKLVRLLVVSEGPLTATKNRLAKDNKDSGATTSGVKTPKWLAPLLLLIDRLEKVAILTKRKQLMHKVTTRAWKWFDLSTGKWTLYSPMNNRIINDAYWAGESSVRISCSRRRYLITFSCMTQVNEDTDNRRPITMCFK-----------LNTGNNEDGGSGSDSNMDTDES------------PIEEKRNTVVQGLDPSIAPSIVRACVKLLAIPVDRDALHALMKVCLRLTRDYKNAEVFAREGGVKLLLEMTHMSSFTGCINLSTLLIRHTLEEPRTLRLAMEKVIRTRTQINIPPAYKEILFMTRQISSAVCRNPEVYKEVCENILRVDISVLKKD----DVDNRLVVKALPPSHSPALPM--VEEVSVGVIRDLLNALIKPMPISSD--------------DGTITPTSPEKKSSHSTSSSAIGNATSSTSSRRDVLRNNTTTTNDPLDDDDQV---SQIIPLKIYT-------DIGSSSKVEPEEAKKPVLTKSAILKILAEAVRSYGAVASLITEHTYRAGQSEMVAEDTTALAFLLDKLLPMLPENSSDRTCSGMARMLIAAIASCNHSPDVQTTLVTEVKAALTRTLALPESSEKHAQLQLLIGLISTMIDSCPPTSHTQLRASLKAHQYNNVNYIVRIMLRKGIITDLAKIPHSLDLSSPNMAATINAALKPLETLSRIINQP-----MPG---AVNNKFTKPKNRTVQEEPIGEQTG--TTTSEATHAVGEEANE-DAENTEHDISVTAESLEPASEGQVH--EEGDEAALEDIMDQLLE 2345
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Match: gi|749752906|ref|XP_011138820.1| (PREDICTED: E3 ubiquitin-protein ligase HUWE1 isoform X3 [Harpegnathos saltator]) HSP 1 Score: 2285.37 bits (5921), Expect = 0.000e+0 Identity = 1289/2580 (49.96%), Postives = 1657/2580 (64.22%), Query Frame = 0
Query: 1 MKIERSKLKKSCSEVPPDCQALIDQLTAAGQAGREAFLRELQRIETWIYGKCELFHWIEVLDLCDEVLEAAARPLHPETAQAWARSADASLEAQVGGAPPAPEAEAGAEAGLEAAKKKELLLWTLHFTTLLIEHSFSRHLYSSMEHLTALLASSDLDVILAVLNLLYMFSKRSNFIARLNAEKRQGLLSRLTYLASSWGGRENGFGLAKCCSDHPIQTFPESATTLNFEYHSEVPAESGEPAGCRSRPKPDPSATTTVTTQIHVAAVDRMGQTPAQIMDDLLVRYASVPETSQMLLFTYLRLAASFSDYPQRLKCVQARLQALSVLIYSNQLTDNVQSLLYPGLLEELVEVLEMSGDHLMEIKAAALKALTSIIHLDRNPHFPK-----LNTIIDVTGAASYHGFLPVLVRNCITSLTANKKDVQPFPQPLATALFSFLYHLASYEAGGEALVSCGMLESLLKVIQWPSTELDHITFVTRAVRVIDLITNLDMQSFQTHAGMTCFINRLELEVEHCRQQQPYEIRLASARRDSLAEALEQQQAEEEAAAAAAACEDVEGEMSPQNTSMELELDPRAVPSTSKALEPTTNYPTAPVPGVSCLPQRSALLKSMLNFLKKAIQDPAFSDSIRHVMDGPLPNSLKHIISNAEYYGASLFLLATDVVTAYVFQEPSLLSSLQDKGLTDVVLHALLVKDVPATREVLGSLPNVFSALCLNTRGLNAFVECKPFERLFKVLLSPDYLPAMRRRRSSDPMGDTASNLGNAMDELMRHQPSLKQSATTAIIKLLEELCTMGRNPKYICSKANSKTEAPVPAEVPSTTNEGGSSDE----------------EEEEDDETSTNQPQDNTSQAEQVEQQTPAPQT---------EKQPVPLVDYIHNVMKFVEAILSNNSTDDHCREFVKQKGLEPLMGILGLPNLPIDFPTHQACQAVAAVSKTVLNLGQEPLVLHQGLLQLNDVLKNLQPLHTPLEPPGGSVLLRELVSSPVIGEATTDPLATPLLHHMSAAHAYIQMFVHVCRTGQNDIRTMSIQHWGSELGLSVLQGLSKLYTSLVWESTVLLALCSEDTLPPGSEFGKADMDKLLPVSTTSPVAGAGSVLAPTAGSSMVANPAEKSTENEETFSPASGTSADSNASVTAAMENLSTDPDIANMEVDGSLPASSSNLMALSGEKSKDKASSVLHQQIKQIKPLLSGSSRLGRALAELFALLVKLCVGSPLRQRRGQPTPLTPATPSPPARAVASSLTKLLAAGLSWDPPATSPIPRFRLTFFICSVGFTAPMLFDEKKFPYHLMLMKFMTSGGQKAFFDTFYWALSCGRTVAPEDGLEHPDLPDGTGEFLDSWLLLLEKMVNPKTILESPHILPSKPTPGFK--PFDPIKYIARTHRMAFEAVMMLWGKKPLKVYGPRMSESVLSILCHILKGEKIIEEKYQKDSIPAPSTASKIPSLTSSLRNPFMPAYNLPPTSQPGGGGTSGAATSSVATSIAAATGGSNTSGAGPSGLAAAPLPQLPPPHINPDHLVRLMDMGFPRERCIDAIQSTESLDQATDYLLNDPFPTTPNVSVGGSSGAPGESLGGTGSAGGSGSTGPGADQDELMRAIAMSLGENVLLSGGSSSGPGEKNIGQKRETGAQISKKPSEEDDEEMADEEFAPLEKSV---LDGFADNALKGCLSLIDTLPDCVFKVCDLLVAVFNRNGLQIKESYLKSLGQEVGQVLNQLNDHAKSEKSFENSMDHLITSPASAQAAARIHLFTLLYE----DCKVLCARMVDSSKVITSMTTLL--------DTFQKVIKGLKD-GASTVQIETPKWMTPMVLFIDLHEKVILGINRRAKLKDVCTRTWKFYDIASGKWCSYLAANNKSIDDAFWAGETSLKIQIGRRKYTIQFGSMLQTNEETGNRRPVMIIIKDRPKPISSEGETATKTDEDDNSPTPTPMDQSESARESNETVGEPNPNGEEVLSTIDGLALEEKRTLLQSCVALIGIPVDHNALNAVLRLCLRLTQDFDHAILFTQLGGIKMLLNLTQESNFPGFASLATLLVRHVMEDPDTLKHTMEKVIRTCTANASTPNNKDLHYLFRALAPAACRAPECFMQVAKDVLRVDLALLNKRGGELEDDHRLILKALPPRAGAPTSMGSLNEVSHSVIADLLNFLVQPE-VESDAAIDSAEANADVNVGGSNVPPTPAVTPGSVSNVSVTNNATNRT---QVIIRNSSS-------GDLQVPATDQAIPSENPTTSKEPKEDNPASTKAEEDLKKRPLLTKSAICRLLAELVKSYGGCAKLITDHVYESGISELIHEETMALAYMLDELL----INKSGKDLGTVVKTLVAAIASCNNQSEAQTTLVSEVKSALARALAWPESNLKHTKIQALTTLVSTMIESCPAAQAIQQMPAFKNQQH-TMNNIVKIMLKKGVIVDLARISHNLDLSSPNVAATVNAALKPLEILTRIVNQPGTLAQRPGPGKAKSSEGSAPDASIAENIDSGTNVGTNTTNSEATRAQGDETVEPDAEATELDISTAAESMDPNSESQLQTVEEGNDEEFDEMMDQILE 2516
MKI+RS+LKK SEVP +CQ LI++L + A L EL++I+ W +GKCELFHWI+VLDL D +LE AA ARS D E P EA KELLLW LHFTTLLIEHSFSRHLY+SMEHL LL+S D+ V+L VLNLLYMFSKRSNFI RLN++KRQ LLSRL +LA SWGG+ENGFGLA+CC ++P + FP SATTL+FE+++E P S S P + T IH+ AVD++G+TPAQIM+DLL Y +VP+ QM L T++RLA SFSDY +RL+CVQARLQALSVL+YSN L +N SLL+ GLLEELVE+LE+ HL+EI+AAAL+ LTSIIHLDRNPHFPK LN IIDVTGA+SYHGFLPVLVR CIT+LT+ + D QPFP PLATALFSFLYHLASYEAGGEALVSCGM+ESLLKVI WP +EL+HITFVTRAVRVIDLITN+DMQ FQTH G+ FINRL++EV CR++QP+EI +D+ + E+ EE ++ + + E + ME D T + E +Y A G +CLPQR+ALLKSMLNFLKKAIQDPAFSDSIRHVM+G LP+SLKHIISN+EYYG SLFLLATDVVT YVFQEPSLLSSLQD GLTDVVLHALL+K+VPATREVLGSLPNVFSALCLN RGL +FV+ +PFERLFKVLLSP YL AMRRRRS+DP+GDTASNLGNAMDELMRHQPSLK A AIIKLLEELC +G +P+YIC +A SK++ P+ + TT SS + DE ++ + +TS Q E+Q EK P+ LV+YIHNVMKFV+AILSNNSTDDHCREFV QKGL PL+ ILGLPNLP+D+P QA Q+VA+V K++LNL EP VL GL QLN+VL L+PLH+ +E PGGSVLL EL S+P + A T ATPLLH M+AAH Y+ MFVHVCRT Q++IR +S+ HWGS++GL+VL GLS LY SLVWEST+LLALCSED +P G EFGK DMDKL+P + G S + TA + M +N +T AME LSTDP MEVD S +G S + + Q+K IKPLL SSRLGRALAELF LLVKLC+GSP+RQRRGQ PLTPA PS AR VASSL LL GLSWD SPIP+F+LTF ICSVGFT+PMLFDEK+ PYHLML KF+ S GQ +FF TF+WALS G A +GLEHP+LPDGTGEFLD+WL+LLEKMVNPK IL+SPH++ +K + FK PF+PIKY+ H+ AFEAVM++WGKKPLK YG RM+ES+LSIL HIL+GEKII+E+ +K N+ G GGTSG A S G S+ P +NP++L +LMDMGF R CI+A+ T +++QATDYLL +P + P +L G G D+LM AIA+SL P IG + EEF E ++ +D F +AL+ CL L+D +PD V+++CDLLV + RNG + ++S L+ L +E+G +++ L A+ + EN+ L+ + + A RI+L+TL +E + +V CA++V+ ++ + LL T ++ K KD GA+T ++TPKW+ P++L ID EKV + R+ + V TR WK++D+++GKW Y NN+ I+DA+WAGE+S++I RR+Y I F M Q NE+T NRRP+ + K T +ED S + + MD ES P E+ + + GL +++++CV L+ IPVD +AL+A++++CLRLT+D+ +A +F + GG+K+LL +T S+F G +L+TLL+RH +E+P TL+ MEKVIRT T P K++ ++ R ++ A CR PE + +V +++LRVD+++L K + D+RL++KALPP M + EVS VI DLLN L++P + SD G+ P +P ++ S NAT+ T + ++RN+++ D QV Q IP + T D +S+K E + K+P+LTKSAI ++LAE V+SYG A LIT+H Y +G SE++ E+T ALA++LD+LL N S + + + L+AAIASCN+ + QTTLV+EVK+AL R LA PES+ KH ++Q L L+STMI+SCP Q + K Q+ +N IV+IML+KG+I DLA+I H+LDLSSPN+AAT+NAALKPLE L+RI+NQP PG A +++ + P + G G TT SEAT A G+E E DAE TE DIS AES++P SE Q+ EEG++ +++MDQ+LE
Sbjct: 1 MKIDRSRLKKCTSEVPTECQTLINKLRSCSHA---ELLEELKQIDAWTFGKCELFHWIDVLDLSDSILEEAA-----------ARSPDNPWELACD-LPQNREA-------------KELLLWVLHFTTLLIEHSFSRHLYNSMEHLVTLLSSCDMHVVLGVLNLLYMFSKRSNFITRLNSDKRQALLSRLNHLAESWGGKENGFGLAECCKEYPEKPFPTSATTLHFEFYAENPTVSEPSTTGTSLPSTKKVGQANLVTYIHIEAVDKLGKTPAQIMNDLLKVY-NVPQERQMALLTHIRLAHSFSDYRRRLQCVQARLQALSVLVYSNALQENAHSLLHAGLLEELVELLELPHTHLVEIRAAALRTLTSIIHLDRNPHFPKKPGSRLNMIIDVTGASSYHGFLPVLVRTCITTLTSPQSDGQPFPLPLATALFSFLYHLASYEAGGEALVSCGMMESLLKVINWPGSELEHITFVTRAVRVIDLITNIDMQGFQTHGGLASFINRLDMEVNVCRKEQPFEIE-PLLYQDTPQTSRERSVDREEESSTSRRVHETFDEREESSNPMEFSEDENMSSKTDEKSEQQPDYSAAKT-GKTCLPQRAALLKSMLNFLKKAIQDPAFSDSIRHVMEGTLPSSLKHIISNSEYYGPSLFLLATDVVTVYVFQEPSLLSSLQDNGLTDVVLHALLIKEVPATREVLGSLPNVFSALCLNQRGLESFVKRRPFERLFKVLLSPVYLSAMRRRRSADPLGDTASNLGNAMDELMRHQPSLKVDAIQAIIKLLEELCVLGCDPRYICWRAASKSDNS-PSNI--TTGNRQSSGQSVGVGVGDSGGAGGGGGGSSSDEEEEDEEEASTSSHPQREEQPSPSPAQPGPPPQPREKTPIALVEYIHNVMKFVDAILSNNSTDDHCREFVGQKGLVPLLSILGLPNLPVDYPVAQAAQSVASVCKSILNLAHEPAVLKTGLSQLNEVLTLLKPLHSHMESPGGSVLLHELASAPNLETAFTSDTATPLLHAMNAAHGYVVMFVHVCRTSQSEIRNLSMNHWGSDVGLAVLDGLSDLYMSLVWESTLLLALCSEDIIPAGCEFGKEDMDKLMPPELKT--EGESSTWSGTASADMGSN------------------------GMTTAMEALSTDPPPVPMEVDDKPNVS-------TGRASPN------NYQLKYIKPLLGASSRLGRALAELFGLLVKLCMGSPVRQRRGQQMPLTPALPSQAARNVASSLNGLLTRGLSWDKLPPSPIPKFKLTFLICSVGFTSPMLFDEKRHPYHLMLHKFVLSDGQSSFFATFHWALSGGGQFALTEGLEHPNLPDGTGEFLDAWLMLLEKMVNPKAILDSPHVVSAKCSGMFKEPPFEPIKYLTEIHKKAFEAVMLMWGKKPLKNYGARMTESILSILRHILRGEKIIKERTEKKE------------------------ENVEGAVASGSGGTSGIARS----------GTSDRRDE-------------PEADVNPEYLRQLMDMGFSRAHCIEALMHTLTVEQATDYLLTNP----------ATLRRPDAALNDVG-----GQVMDVETDDDLMHAIAVSL-----------DSPDAHKIG--------------------VGIEEFGR-ETTITENIDEFTHSALEQCLHLLDLMPDTVYRICDLLVTITKRNGDEWRDSMLRQLIREIGILVDHLIQIAEGQD--ENAGTRLVECEEANKVAGRIYLYTLFFEGTFQEMRVPCAQIVEECAILDKLVRLLVVSEGPLTATKNRLAKDNKDSGATTSGVKTPKWLAPLLLLIDRLEKVAILTKRKQLMHKVTTRAWKWFDLSTGKWTLYSPMNNRIINDAYWAGESSVRISCSRRRYLITFSCMTQVNEDTDNRRPITMCFK-----------LNTGNNEDGGSGSDSNMDTDES------------PIEEKRNTVVQGLDPSIAPSIVRACVKLLAIPVDRDALHALMKVCLRLTRDYKNAEVFAREGGVKLLLEMTHMSSFTGCINLSTLLIRHTLEEPRTLRLAMEKVIRTRTQINIPPAYKEILFMTRQISSAVCRNPEVYKEVCENILRVDISVLKKD----DVDNRLVVKALPPSHSPALPM--VEEVSVGVIRDLLNALIKPMPISSD--------------DGTITPTSPEKKSSHSTSSSAIGNATSSTSSRRDVLRNNTTTTNDPLDDDDQV---SQIIPLKIYT-------DIGSSSKVEPEEAKKPVLTKSAILKILAEAVRSYGAVASLITEHTYRAGQSEMVAEDTTALAFLLDKLLPMLPENSSDRTCSGMARMLIAAIASCNHSPDVQTTLVTEVKAALTRTLALPESSEKHAQLQLLIGLISTMIDSCPPTSHTQLRASLKAHQYNNVNYIVRIMLRKGIITDLAKIPHSLDLSSPNMAATINAALKPLETLSRIINQP-----MPG---AVNNKFTKPKNRTVQEEPIGEQTG--TTTSEATHAVGEEANE-DAENTEHDISVTAESLEPASEGQVH--EEGDEAALEDIMDQLLE 2345 The following BLAST results are available for this feature:
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 2
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 8
BLAST of hypothetical protein DAPPUDRAFT_313219 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold450_size166944:44809..54726+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold450_size166944-snap-gene-0.20 ID=maker-scaffold450_size166944-snap-gene-0.20|Name=hypothetical protein DAPPUDRAFT_313219|organism=Tigriopus kingsejongensis|type=gene|length=9918bp|location=Sequence derived from alignment at scaffold450_size166944:44809..54726+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'hypothetical protein DAPPUDRAFT_313219' has the following synonyms
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