BLAST of transmembrane protease serine 9-like vs. L. salmonis genes
Match: EMLSAG00000010392 (supercontig:LSalAtl2s:LSalAtl2s68:1354764:1380961:1 gene:EMLSAG00000010392 transcript:EMLSAT00000010392 description:"maker-LSalAtl2s68-augustus-gene-13.10")

HSP 1 Score: 330.872 bits (847), Expect = 3.219e-104
Identity = 198/545 (36.33%), Postives = 288/545 (52.84%), Query Frame = 0

Query:   38 NPFGILGHKICETNVRGEDVTGVCFNEVECFIKGGAHSGYCSPSPLSGVCCAFVNKIC--DRTVNHKVSYFTNPSYPKADSAAQ--------------LKLDYEALELASDGNGNCLHDSLTIINNVDGPTGRICGNKKGYSQVVR---TTPGGSLKLAVVAQSPQHRW----------RVSPVQDDVSCGETGAEGFIFRRNSDTKRSRSHHHKGLRKVSKIRKRIPRMTILDFYNNAMPISSRCGKSHPPETCRSPKSEDEVAEDTEELTKKRLFHELYNPSFSAKGVFCDSKFRNKEYLGPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEY--NRAIKPICLPKLEAGGSFEQMLA-VAAGWGVTEDQSSSDILRQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYFKR 550
            N F    ++ C T+     + GVC+N+++C  +GG  SGYCS + LS VCC FV K C  + +   K  YFTNP YP  DS A               +K++Y   +L S  NG C ++ LTIIN   G    ICG K   + ++     T   ++K+ ++ Q+ ++ W          ++ P+  D SCG TGA   +  R+ +T  S                  PR  +   Y           K H  +   +             L +++L  E+  P F+ +G FC S F + E+   S KII+G +AP+ AFPW+VS  Y G+HFCGGS+I+   V+TAAHC DF  + +F   + V  G+HD+SL+NET  +T R +  I I+P +   PGS GD+AL++L+ PV    ++AI  +C+P   +    E M + +AAGWG+TE    S +LR VELN I  S C +NM  + + I + M+CT KG    ET+CSGDSG P M R   +F + G+ SFS TDC+APFPAV+ R+S F +
Sbjct:   20 NLFSYEANRSCLTS----SLKGVCYNKLQCLARGGQISGYCSNALLSEVCCVFVQKGCYSNPSSQEKSLYFTNPKYPDYDSEANSCYLTVYFQPNVCWIKIEYNNFQLPSSKNGLCHYNYLTIINGGSGSGSMICGFKSRMAXLIERPXKTKENAIKINILTQAEKYAWNIRVVQIECDQIQPLPRDPSCGFTGAPNSLVARSLNTLES------------------PRNXLRHIY-----------KPHGGDGALN-------LNRISPLLRRKLNTEMNYPGFNIRGSFCKSIFHD-EFTEASEKIINGVEAPVFAFPWIVSFQYRGDHFCGGSIIDPQWVMTAAHCFDFPNLHEFLEGVEVSIGDHDISLSNETR-STIRTIERIWIYPAWYRNPGSDGDLALIKLESPVVNIGSKAIHSVCMPDEHSVSKLESMKSGIAAGWGITESGDKSKVLRMVELNRIPLSVCKKNMEPLLIDIKKGMLCTLKGTNSRETVCSGDSGSPFMARYGSRFFVIGVVSFSTTDCNAPFPAVYTRVSQFVK 522          

HSP 2 Score: 171.014 bits (432), Expect = 1.431e-45
Identity = 100/282 (35.46%), Postives = 157/282 (55.67%), Query Frame = 0

Query:  552 RRPRSIDKQFP-YRSRPYQPTSAFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEF---IRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPF--NQGIKPVCLPTNSPVGTFDN-RMGIAAGWGLTENETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWISAALS 826
            RR  + +  +P +  R     S F  + +    KI++G ++ + AFPW  S  + G  H CGGS+I    ++TAAHC + P   EF   + V++G+HD+S  +ET  T+ R ++R+ ++P++     G  GD+ALI LE PV    ++ I  VC+P    V   ++ + GIAAGWG+TE+     V+R V+LN I L  C      L  +++   M+CT  G     + C GDSG P M   G++  ++GV SF T +C+ P  +VY+RV+ F+ W  A +S
Sbjct:  252 RRKLNTEMNYPGFNIRGSFCKSIFHDEFTEASEKIINGVEAPVFAFPWIVSFQYRGD-HFCGGSIIDPQWVMTAAHCFDFPNLHEFLEGVEVSIGDHDISLSNETRSTI-RTIERIWIYPAWYRNP-GSDGDLALIKLESPVVNIGSKAIHSVCMPDEHSVSKLESMKSGIAAGWGITESGDKSKVLRMVELNRIPLSVCKKNMEPLL-IDIKKGMLCTLKGTNSRETVCSGDSGSPFMARYGSRFFVIGVVSFSTTDCNAPFPAVYTRVSQFVKWXGAVIS 529          

BLAST of transmembrane protease serine 9-like vs. L. salmonis genes
Match: EMLSAG00000011454 (supercontig:LSalAtl2s:LSalAtl2s790:183804:208736:-1 gene:EMLSAG00000011454 transcript:EMLSAT00000011454 description:"maker-LSalAtl2s790-augustus-gene-2.32")

HSP 1 Score: 137.502 bits (345), Expect = 1.775e-33
Identity = 90/251 (35.86%), Postives = 132/251 (52.59%), Query Frame = 0

Query:  583 LKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVE--TPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFD-NRMGIAAGWG-LTENETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWISAALSVFS 829
            LKIV+G  +  H +PW A+L F  G   CGGSLI    I+TAAHCV   T      + V LGEH++    +T   V R+V ++I H  +++    L  D+AL+ L DPV ++  IKP+C+ T+     +D N      GWG L E+   P ++++V L +   E+C   +       +T  MIC   G  G  S C GDSGGP++  +G K V +G+ S+G          VY+RV    +WI+  +  FS
Sbjct:  661 LKIVNGWPASKHEWPWIAAL-FNRGRQFCGGSLISRSHILTAAHCVAQMTRSDVNNLEVRLGEHNIKDALDTQVEV-RKVNKIIRHKGFSSQT--LWNDVALLFLSDPVSYSDIIKPICIDTSD--DPYDKNXTATVVGWGSLRESGPQPXILQEVTLRLWKNEKCQETYNGASPAGITDHMICA--GRQGKDS-CSGDSGGPMVKAKGDKWVQIGIVSWGIGCGKSHFPGVYTRVGKMYEWITKVIEKFS 902          

HSP 2 Score: 125.946 bits (315), Expect = 8.437e-30
Identity = 84/242 (34.71%), Postives = 127/242 (52.48%), Query Frame = 0

Query:  306 NKEYLGPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAIN-VPILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARI 545
            N+  +    KI++G  A  H +PW+ +L   G  FCGGSLI+++ ++TAAHC+      D  + L V  GEH++  A +T+    R+V  II H  +S+      DVALL L  PV Y+  IKPIC+   +     +   A   GWG + E      IL++V L + +N  C E  N  +   I + MIC   G  G ++ CSGDSGGP++     K+   G+ S+ +    + FP V+ R+
Sbjct:  653 NRIDMEHDLKIVNGWPASKHEWPWIAALFNRGRQFCGGSLISRSHILTAAHCVAQMTRSD-VNNLEVRLGEHNIKDALDTQ-VEVRKVNKIIRHKGFSSQT-LWNDVALLFLSDPVSYSDIIKPICIDTSDDPYD-KNXTATVVGWGSLRESGPQPXILQEVTLRLWKNEKCQETYNGASPAGITDHMICA--GRQGKDS-CSGDSGGPMVKAKGDKWVQIGIVSWGIGCGKSHFPGVYTRV 887          

HSP 3 Score: 97.0561 bits (240), Expect = 8.372e-21
Identity = 74/241 (30.71%), Postives = 118/241 (48.96%), Query Frame = 0

Query:  297 GVFCDSKFRNKEYLGPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLAN---ETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQS-SSDILRQVELNIIRNSTCVENMNAINVP--ILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSV 531
            GV   S    + +   + KI++G DA +  FP++ +L     HF GGS+I+   ++TAAHC+      D    L V+  ++ +  +N   E EF    RV  +I H  +SA      DVA+L L   ++++++++PICL   +   ++E  +   AGWG   D    S IL++V + +  N  C  +  +   P  I   M+C       S   CSGDSGGPL    D   T  G+ S+ +
Sbjct:  233 GVKLSSDHCGQRFFSNNLKIVNGEDASVDEFPFMAALFNKXRHFXGGSIIDSKHILTAAHCVAHMTKID-VRYLRVHVRQYRIKSSNIGTEQEF----RVRRVIRHKSFSANT-LYNDVAILTLRGSIDFSKSVQPICLAS-DNTETYEGDIVTVAGWGTLSDGGRQSSILQKVSVGVWSNYDCGASYGS-RAPGGIKSHMLCAASKGKDS---CSGDSGGPLFY-CDTYCTQIGIVSWGI 461          

HSP 4 Score: 90.1225 bits (222), Expect = 9.847e-19
Identity = 66/223 (29.60%), Postives = 116/223 (52.02%), Query Frame = 0

Query:  583 LKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVE--TPGFAEFIRVTLGEHDLSRPD---ETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWG-LTENETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFG 799
            LKIV+G  + +  FP+ A+L F    H  GGS+I +  I+TAAHCV   T     ++RV + ++ +   +   E  F    RV+RVI H S++A    L  D+A++ L   + F++ ++P+CL +++   T++  +   AGWG L++      ++++V + + S   C   +       +   M+C  +       +C GDSGGPL   + T    +G+ S+G
Sbjct:  250 LKIVNGEDASVDEFPFMAAL-FNKXRHFXGGSIIDSKHILTAAHCVAHMTKIDVRYLRVHVRQYRIKSSNIGTEQEF----RVRRVIRHKSFSANT--LYNDVAILTLRGSIDFSKSVQPICLASDN-TETYEGDIVTVAGWGTLSDGGRQSSILQKVSVGVWSNYDCGASYGSRAPGGIKSHMLCAAS---KGKDSCSGDSGGPLFYCD-TYCTQIGIVSWG 460          

BLAST of transmembrane protease serine 9-like vs. L. salmonis genes
Match: EMLSAG00000007886 (supercontig:LSalAtl2s:LSalAtl2s464:52646:53754:1 gene:EMLSAG00000007886 transcript:EMLSAT00000007886 description:"maker-LSalAtl2s464-augustus-gene-0.4")

HSP 1 Score: 125.561 bits (314), Expect = 2.120e-32
Identity = 85/244 (34.84%), Postives = 134/244 (54.92%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFA-EFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTN--SPVGTFDNRMGIAAGWG-LTENETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGS-TCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDP-VSSVYSRVTYFMDWI 821
            KIV GT+++ H+ P+  S     GFH CGGS++    +ITA HC +  GF+   ++V +G HD + P+ T    T+ + ++  H ++ +   G+  DI L+ +E P  FN  +KPV LP    +P G       + +GWG L  N  +  V+R V LN++   +C   +  + GL+   +MIC      G G  +C+GDSGGPL+     +  LVG+ S+G   C+ P    VY++V+ F+DWI
Sbjct:   38 KIVGGTEAQPHSIPFQVSFQRKNGFHFCGGSILDETTVITAGHCCK--GFSINDVQVVVGAHDFNSPEGTE--QTQNIVKITYHENFAS--KGINNDICLLEVEHPFEFNDNVKPVTLPEKEFTPTGEV-----VVSGWGTLRANGNSSPVLRTVTLNMVPYLRCYINY--IGGLD--ESMICA----SGKGKDSCQGDSGGPLV----QENTLVGIVSWGI-GCAHPWFPGVYTKVSMFIDWI 257          

HSP 2 Score: 122.865 bits (307), Expect = 1.743e-31
Identity = 89/244 (36.48%), Postives = 135/244 (55.33%), Query Frame = 0

Query:  309 YLGPSFKIIDGTDAPIHAFPWLVSLN-YNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINVPILEDMIC-TFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAP-FPAVFARISYF 548
            Y+G S KI+ GT+A  H+ P+ VS    NG HFCGGS++++ TVITA HC    ++ D    + V  G HD +    TE   T+ ++ I  H  + A+ G   D+ LL+++ P E+N  +KP+ LP+ E   + E    V +GWG +  + +SS +LR V LN++    C  N       + E MIC + KG    +  C GDSGGPL+     + TL G+ S+ +  C+ P FP V+ ++S F
Sbjct:   33 YMGES-KIVGGTEAQPHSIPFQVSFQRKNGFHFCGGSILDETTVITAGHCCKGFSIND----VQVVVGAHDFNSPEGTE--QTQNIVKITYHENF-ASKGINNDICLLEVEHPFEFNDNVKPVTLPEKEFTPTGE---VVVSGWGTLRANGNSSPVLRTVTLNMVPYLRCYINYIG---GLDESMICASGKG----KDSCQGDSGGPLV----QENTLVGIVSWGI-GCAHPWFPGVYTKVSMF 253          

BLAST of transmembrane protease serine 9-like vs. L. salmonis genes
Match: EMLSAG00000008660 (supercontig:LSalAtl2s:LSalAtl2s537:130430:177832:-1 gene:EMLSAG00000008660 transcript:EMLSAT00000008660 description:"maker-LSalAtl2s537-augustus-gene-2.26")

HSP 1 Score: 130.183 bits (326), Expect = 3.928e-31
Identity = 81/250 (32.40%), Postives = 129/250 (51.60%), Query Frame = 0

Query:  579 SNVLLKIVDGTQSRLHAFPWAASLSFVGGFHG-CGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWG-LTENETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDP-VSSVYSRVTYFMDWISAAL 825
             NV  +IV G +   + +PW   +  +G     CGG+L+ +  ++TA HC +T      + + LGEH++   ++T+  +   VK+V +H  ++   +    DI L+ LE P+ F + I+P+CLP       F   M    GWG L E    PD++++V++ +I+ + C   +  L     TP MIC      G    C+GDSGGPL   +  + VL G TS+G   C+ P    VYSR+T   DWI+  +
Sbjct:  822 KNVNSRIVGGKEVEENEYPWHIGIVKIGDNKPFCGGALVTDRHVVTAGHCFQT---FHKVEILLGEHNIQNSNDTALRIP--VKKVEVHKEFSVLAMN-DYDITLLTLERPIKFRREIQPICLPFKDE--DFVGTMCTITGWGRLNETGPQPDILQEVNVPVITNDVCKKSYETL-----TPRMICA-GYKKGELDACQGDSGGPLSCPQNNRYVLAGSTSYGF-GCARPDFPGVYSRITLLQDWINERI 1056          

HSP 2 Score: 129.028 bits (323), Expect = 1.141e-30
Identity = 82/277 (29.60%), Postives = 134/277 (48.38%), Query Frame = 0

Query:  284 LFHELYNPSFSAKGVFCDSKFRNKEYL------GPSFKIIDGTDAPIHAFPWLVSLNYNGEH--FCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINVPILEDMICT--FKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYFK 549
            LF   YN  F  KG  C  K + +++         + +I+ G +   + +PW + +   G++  FCGG+L+    V+TA HC         F K+ +  GEH++  +N+T       V  + +H  +S    +  D+ LL L+RP+++ R I+PICLP  +    F   +    GWG + E     DIL++V + +I N  C ++   +       MIC    KG L +   C GDSGGPL    + ++ LAG  S+        FP V++RI+  +
Sbjct:  790 LFFNFYNKYFQNKGFMCVVKAQKEDFKCKCGTKNVNSRIVGGKEVEENEYPWHIGIVKIGDNKPFCGGALVTDRHVVTAGHCFQT------FHKVEILLGEHNIQNSNDTALRIP--VKKVEVHKEFSVLAMNDYDITLLTLERPIKFRREIQPICLPFKDE--DFVGTMCTITGWGRLNETGPQPDILQEVNVPVITNDVCKKSYETLT----PRMICAGYKKGELDA---CQGDSGGPLSCPQNNRYVLAGSTSYGFGCARPDFPGVYSRITLLQ 1049          

HSP 3 Score: 127.102 bits (318), Expect = 4.291e-30
Identity = 81/250 (32.40%), Postives = 128/250 (51.20%), Query Frame = 0

Query:  579 SNVLLKIVDGTQSRLHAFPWAASLSFVGGFHG-CGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWG-LTENETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDP-VSSVYSRVTYFMDWISAAL 825
             NV  +IV G +   + +PW   +  +G     CGGSL+ +  ++TAAHC +T      + V LGEH++   ++T+  +   VK+V +H  +         D+ L+ LE P+ F + I+P+CLP       F   M    GWG L+E E  PD++++V++ +I+ + C   +  +     TP MIC      G   +C+GDS  PL   +  + V  G+TSFG   C+ P    VYSR+T   DWI   +
Sbjct:  157 KNVNSRIVGGKEVEENEYPWQIGIVKIGKNKPFCGGSLVSDRHVVTAAHCFKT---LYKVEVLLGEHNIQNSNDTALRIP--VKKVEVHKEFGVLAFN-DYDVTLLTLEQPIKFRREIQPICLPFKDE--DFVGTMCTITGWGRLSETEPQPDILQEVNVPVITNDVCNKAYETI-----TPRMICA-GYEKGEIDSCQGDS--PLSCPQNNRYVFTGITSFG-HGCARPGFPGVYSRITLLQDWIKERI 389          

HSP 4 Score: 120.553 bits (301), Expect = 5.157e-28
Identity = 86/287 (29.97%), Postives = 139/287 (48.43%), Query Frame = 0

Query:  284 LFHELYNPSFSAKGVFCDSKFRNKEYL------GPSFKIIDGTDAPIHAFPWLVSLNYNGEH--FCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINVPILEDMICT--FKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAP-FPAVFARISYFKRRRRPRSID 558
            L+ + YN  F  KG  C  K + +E+         + +I+ G +   + +PW + +   G++  FCGGSL++   V+TAAHC           K+ V  GEH++  +N+T       V  + +H  +     +  DV LL L++P+++ R I+PICLP  +    F   +    GWG ++E +   DIL++V + +I N  C    N     I   MIC    KG + S   C GDS  PL    + ++   G+ SF    C+ P FP V++RI+  +   + R  D
Sbjct:  125 LYFDFYNKYFQNKGFMCVIKAQEEEFKCKCGTKNVNSRIVGGKEVEENEYPWQIGIVKIGKNKPFCGGSLVSDRHVVTAAHCFKT------LYKVEVLLGEHNIQNSNDTALRIP--VKKVEVHKEFGVLAFNDYDVTLLTLEQPIKFRREIQPICLPFKDE--DFVGTMCTITGWGRLSETEPQPDILQEVNVPVITNDVC----NKAYETITPRMICAGYEKGEIDS---CQGDS--PLSCPQNNRYVFTGITSFG-HGCARPGFPGVYSRITLLQDWIKERIGD 391          

HSP 5 Score: 95.9005 bits (237), Expect = 1.820e-20
Identity = 58/199 (29.15%), Postives = 98/199 (49.25%), Query Frame = 0

Query:  284 LFHELYNPSFSAKGVFCDSKFRNKEYL------GPSFKIIDGTDAPIHAFPWLVSLNYNGEH--FCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRN 473
            LF    N  F  KG  C  K + +E+         + +I+ G +   + +PW + +   GE+  FCGG+L+    V+TA HC         F K+ +  GEH++  +++T      RV  + +H  ++    +  D+ LL L+RP+++ R I+PICLP  +    F   +    GWG + E     DIL++V + +I N
Sbjct:  496 LFFNFNNKYFQNKGFMCIIKAQKEEFKCKCGTKNVNSRIVGGKEVEENEYPWHIGIVKIGENLPFCGGALVTDRHVVTAGHCFQT------FRKVEILLGEHNIRNSHDTALRI--RVKKVEVHKEFNIIAKNDYDITLLTLERPIKFRREIQPICLPFKDD--DFVGTMCTITGWGRLNETGPQPDILQEVNVPVITN 684          

HSP 6 Score: 86.2705 bits (212), Expect = 1.821e-17
Identity = 54/177 (30.51%), Postives = 93/177 (52.54%), Query Frame = 0

Query:  579 SNVLLKIVDGTQSRLHAFPWAASLSFVG-GFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWG-LTENETNPDVIRQVDLNIISLEQ-----CTTEW 748
             NV  +IV G +   + +PW   +  +G     CGG+L+ +  ++TA HC +T  F + + + LGEH++    +T+  +  RVK+V +H  +   +     DI L+ LE P+ F + I+P+CLP       F   M    GWG L E    PD++++V++ +I+ +      C TE+
Sbjct:  528 KNVNSRIVGGKEVEENEYPWHIGIVKIGENLPFCGGALVTDRHVVTAGHCFQT--FRK-VEILLGEHNIRNSHDTALRI--RVKKVEVHKEFN-IIAKNDYDITLLTLERPIKFRREIQPICLPFKDD--DFVGTMCTITGWGRLNETGPQPDILQEVNVPVITNDDFQSAFCLTEY 696          

BLAST of transmembrane protease serine 9-like vs. L. salmonis genes
Match: EMLSAG00000002149 (supercontig:LSalAtl2s:LSalAtl2s139:22566:31405:1 gene:EMLSAG00000002149 transcript:EMLSAT00000002149 description:"maker-LSalAtl2s139-augustus-gene-0.10")

HSP 1 Score: 121.709 bits (304), Expect = 2.392e-29
Identity = 85/255 (33.33%), Postives = 127/255 (49.80%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSFVGGFH---GCGGSLIRNDMIITAAHCVETPGFAEF----IRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMG---IAAGWGLTE---NETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTG--STCRGDSGGPLMVLEGTKQ--VLVGVTSFGTQNCSDPVSSVYSRVTYFMDWI 821
            +IV G  +    +PW A++   GG      CGG+L+    +ITAAHC +     +F      + LGE DL   D+ +++    +  VI HP + A   G   D+A++   +PV F++ I+P+CLPT S     DN +G   +A GWG T     E N   +R V L + + + C       DG    P           +G    C+GDSGGPLM+    ++  VL+GV SFG +        VY+R+T+F+DWI
Sbjct:  253 RIVGGKATYEGEYPWMAAIYLHGGGRTEFWCGGALVSERHVITAAHCTKDAKKKQFKARQFTIRLGEWDLD--DKETYSREYEIVDVIAHPDFKA--NGFYSDVAILKTIEPVEFSEYIQPICLPTGSMKN--DNFLGTLPVALGWGATHYRGKEVNK--LRGVALPVWNNKDC-------DGAYFQPITEVFLCAGYASGGRDACQGDSGGPLMLYSQKRKAWVLIGVVSFGNRCAEAGYPGVYTRLTHFLDWI 492          

HSP 2 Score: 118.242 bits (295), Expect = 3.478e-28
Identity = 79/246 (32.11%), Postives = 122/246 (49.59%), Query Frame = 0

Query:  312 PSFKIIDGTDAPIHAFPWLVSLNYNG----EHFCGGSLINQNTVITAAHCMDFGAVPDFFSK-LLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLE-AGGSFEQMLAVAAGWGVTEDQSSS-DILRQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRIDGK--FTLAGLASFSVTDCSAPFPAVFARISYF 548
            P  +I+ G       +PW+ ++  +G    E +CGG+L+++  VITAAHC        F ++   +  GE DL   ++  ++    ++++I HP + A  G   DVA+L+   PVE++  I+PICLP       +F   L VA GWG T  +    + LR V L +  N  C     A   PI E  +C      G    C GDSGGPLM+    +  + L G+ SF      A +P V+ R+++F
Sbjct:  250 PQSRIVGGKATYEGEYPWMAAIYLHGGGRTEFWCGGALVSERHVITAAHCTKDAKKKQFKARQFTIRLGEWDLD--DKETYSREYEIVDVIAHPDFKAN-GFYSDVAILKTIEPVEFSEYIQPICLPTGSMKNDNFLGTLPVALGWGATHYRGKEVNKLRGVALPVWNNKDC---DGAYFQPITEVFLCAGYAS-GGRDACQGDSGGPLMLYSQKRKAWVLIGVVSFGNRCAEAGYPGVYTRLTHF 488          

BLAST of transmembrane protease serine 9-like vs. L. salmonis genes
Match: EMLSAG00000003879 (supercontig:LSalAtl2s:LSalAtl2s210:105242:137220:1 gene:EMLSAG00000003879 transcript:EMLSAT00000003879 description:"maker-LSalAtl2s210-augustus-gene-1.10")

HSP 1 Score: 122.094 bits (305), Expect = 8.921e-29
Identity = 87/252 (34.52%), Postives = 126/252 (50.00%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPG-DVALLQLDRPVEYNRAIKPICLPKLEAG-GSFEQMLAVAAGWG--------------VTEDQSSSDILRQVELNIIRNSTC--VENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRI-DGKFTLAGLASFSVTDCSAPF-PAVFARIS 546
            +++DG  A   +FPW   +   G+  CGG LIN+  V+TA HC+    +    S L V  GE+ L LANE   +   RV   ++HPR+  +P +   DVA+L +DRPV+Y   I PICLP  EAG        A  AGWG              +  +     +L+ V + ++ N  C         N+ I  +M+C      G ++ C GDSGGPLMV+  DG++ L G+ S   + C  P  P ++ RIS
Sbjct:  480 RVLDGKPAGFGSFPWQALIRI-GKGKCGGVLINRRHVVTAGHCVKNKPL----SSLTVTLGEYHLKLANEPYPSEKFRVTRAVVHPRFQFSPAADRYDVAVLTIDRPVQYAPHIAPICLP--EAGRXPAPGTNAYVAGWGALIPDDVAGPLIPFLLPEIKRPSVLQVVNVPVLDNERCETWHKDKGFNIKIWPEMVCAGYRQGGKDS-CKGDSGGPLMVQQRDGRWVLVGIVSAGFS-CGKPGQPGIYHRIS 722          

HSP 2 Score: 108.997 bits (271), Expect = 1.193e-24
Identity = 87/308 (28.25%), Postives = 141/308 (45.78%), Query Frame = 0

Query:  533 DCSAPFPAVFARISYFKRRRRPRSIDKQFPYRSRPYQPTSAFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWG--------------LTENETNPDVIRQVDLNIISLEQCTTEWRLLDG--LELTPAMICTFNGPLGTGSTCRGDSGGPLMVLE-GTKQVLVGVTSFGTQNCSDPVS-SVYSRVTYFMDWIS 822
            D S+ + +   +I +   R  P   D   P+  RP + T+           +++DG  +   +FPW A +    G   CGG LI    ++TA HCV+    +  + VTLGE+ L   +E   +   RV R ++HP +         D+A++ ++ PV +   I P+CLP               AGWG              L      P V++ V++ ++  E+C T W    G  +++ P M+C      G   +C+GDSGGPLMV +   + VLVG+ S G  +C  P    +Y R++   DW++
Sbjct:  441 DSSSQYRSNHKKIKFLGSRYGPVVND---PHCGRPVELTAN---------RRVLDGKPAGFGSFPWQALIRI--GKGKCGGVLINRRHVVTAGHCVKNKPLSS-LTVTLGEYHLKLANEPYPSEKFRVTRAVVHPRFQFSPAADRYDVAVLTIDRPVQYAPHIAPICLPEAGRXPA-PGTNAYVAGWGALIPDDVAGPLIPFLLPEIKRPSVLQVVNVPVLDNERCET-WHKDKGFNIKIWPEMVCA-GYRQGGKDSCKGDSGGPLMVQQRDGRWVLVGIVSAGF-SCGKPGQPGIYHRISSTSDWVT 729          

HSP 3 Score: 103.605 bits (257), Expect = 5.839e-23
Identity = 87/242 (35.95%), Postives = 116/242 (47.93%), Query Frame = 0

Query:  313 SFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPG-DVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQSS--SDILRQVELNIIRNSTCVE---NMNAINVPILEDMICT-FKGPLGSETICSGDSGGPLMVR--IDGKFTLAGLASFSVTDCSAPFPAVFARI 545
            S +I+ G +A    FPW   +   G   CGG+LI+   V+TA HC++        S + VY GE+ L    E        V  I +HP Y  TP +   DVALL+L+  V Y   I PICLP L+     E  +   AGWG     S+   + L+ V++ II NS   E       I+V I  DM C  +K   G    C GDSGGPL+V+    G++ L GL        SA  P +FA I
Sbjct:  138 SRRIVGGXEAGFGNFPWQALIRI-GTSRCGGALISPYYVVTAGHCINSAKA----SSIRVYLGEYTLYRRIEPLPREKYGVSEIELHPYYRFTPQADRYDVALLRLNTRVNYQPHISPICLP-LKNEVYKEGTIGTVAGWGALSPTSTIRPNKLQAVDVQII-NSDRXEMWHRQKGIHVRIFSDMFCAGYK--EGKRDACQGDSGGPLVVKDIRKGRWVLIGLV-------SAGLPLIFALI 363          

HSP 4 Score: 94.7449 bits (234), Expect = 3.759e-20
Identity = 74/222 (33.33%), Postives = 108/222 (48.65%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWG-LTENET-NPDVIRQVDLNIISLEQCTTEWRLLDGLELT--PAMICTFNGPLGTGSTCRGDSGGPLMV--LEGTKQVLVGVTSFG 799
            +IV G ++    FPW A +    G   CGG+LI    ++TA HC+ +   A  IRV LGE+ L R  E        V  + LHP Y         D+AL+ L   V +   I P+CLP  + V   +  +G  AGWG L+   T  P+ ++ VD+ II+ ++    W    G+ +     M C      G    C+GDSGGPL+V  +   + VL+G+ S G
Sbjct:  140 RIVGGXEAGFGNFPWQALIRI--GTSRCGGALISPYYVVTAGHCINS-AKASSIRVYLGEYTLYRRIEPLPREKYGVSEIELHPYYRFTPQADRYDVALLRLNTRVNYQPHISPICLPLKNEVYK-EGTIGTVAGWGALSPTSTIRPNKLQAVDVQIINSDR-XEMWHRQKGIHVRIFSDMFCA-GYKEGKRDACQGDSGGPLVVKDIRKGRWVLIGLVSAG 355          

BLAST of transmembrane protease serine 9-like vs. L. salmonis genes
Match: EMLSAG00000012442 (supercontig:LSalAtl2s:LSalAtl2s914:161702:164343:-1 gene:EMLSAG00000012442 transcript:EMLSAT00000012442 description:"maker-LSalAtl2s914-augustus-gene-2.11")

HSP 1 Score: 120.168 bits (300), Expect = 1.126e-28
Identity = 83/244 (34.02%), Postives = 133/244 (54.51%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASL---SFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWG-LTENETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQV-LVGVTSFGTQNCSDP-VSSVYSRVTYFMDWI 821
            +IV G  +  + +PW A++   S V     CG +LI +  +ITAAHCV  P   + I+V LGE++ +   ET    T  ++ + +H  Y A  +    DIA++ L+ PV F++ I P+CLP   P+ T++ R G   GWG +       +++++V++ +    +C   +  L+   +T  M+C   G  G    C+GDSGGPL  L    Q  L GV S+G + C++P    VY+RVT ++DWI
Sbjct:  291 RIVGGRPADPNEWPWLAAMIRPSEVSSGQFCGATLISDRHVITAAHCV-APFKQDQIKVRLGEYNFNAEGET-LDRTYNLESIKMHELYDA--VKYYNDIAIMKLDRPVTFSKSIYPICLP--PPIETYEKRRGYVIGWGTIYFGGPTSNILQEVNVAVWDNNKCKENYSALN-RNVTSKMLCA--GDRGR-DACQGDSGGPLNCLNAFSQWELCGVVSWGAR-CAEPDYPGVYTRVTEYLDWI 523          

HSP 2 Score: 116.701 bits (291), Expect = 1.769e-27
Identity = 77/243 (31.69%), Postives = 129/243 (53.09%), Query Frame = 0

Query:  313 SFKIIDGTDAPIHAFPWLVSLNYNGE----HFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICL-PKLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPL-MVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYF 548
            S +I+ G  A  + +PWL ++    E     FCG +LI+   VITAAHC+     P    ++ V  GE++ +   ET    T  + +I +H  Y A      D+A+++LDRPV ++++I PICL P +E   ++E+      GWG +     +S+IL++V + +  N+ C EN +A+N  +   M+C   G  G +  C GDSGGPL  +    ++ L G+ S+        +P V+ R++ +
Sbjct:  289 STRIVGGRPADPNEWPWLAAMIRPSEVSSGQFCGATLISDRHVITAAHCV----APFKQDQIKVRLGEYNFNAEGET-LDRTYNLESIKMHELYDAVKYY-NDIAIMKLDRPVTFSKSIYPICLPPPIE---TYEKRRGYVIGWGTIYFGGPTSNILQEVNVAVWDNNKCKENYSALNRNVTSKMLCA--GDRGRDA-CQGDSGGPLNCLNAFSQWELCGVVSWGARCAEPDYPGVYTRVTEY 519          

BLAST of transmembrane protease serine 9-like vs. L. salmonis genes
Match: EMLSAG00000008852 (supercontig:LSalAtl2s:LSalAtl2s552:1363:4087:1 gene:EMLSAG00000008852 transcript:EMLSAT00000008852 description:"maker-LSalAtl2s552-augustus-gene-0.5")

HSP 1 Score: 118.627 bits (296), Expect = 1.697e-28
Identity = 82/253 (32.41%), Postives = 127/253 (50.20%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSF-VGGFHG----CGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPT-NSPVGTFDNRMGIAAGWGLTENETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEG----TKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWISAALS 826
            +IV G  S LHA+PW A+L + V G       CGG+LI    ++TAAHCV     ++  +V LGEHDL   ++ +      + + I+HP Y    I    DIA+++L + V F+  I P+CLP      GT     G+         + +P  + +++L IIS  +C+  +     + +T   +C  +   G    C+GDSGGPLM  +G    +   L GV SFG +        VY+RV+ +++WI    S
Sbjct:  202 RIVGGKPSELHAWPWIAALGYRVSGSKDSDFLCGGTLISKRHVVTAAHCVFR--RSDLSKVRLGEHDLEDENDGAQPRDYGIIKTIIHPDYHP--IRFNNDIAILVLSNDVEFDHRITPICLPDLMKDSGTSGFSFGLTKQVRDRLLDAHP-FVAEINLEIISNRECSRAFTNFRNVNVTENKLCALDQN-GEKDACQGDSGGPLMTSQGSIAKSNWFLAGVVSFGYRCGVKGFPGVYTRVSEYVNWIKQETS 448          

HSP 2 Score: 107.457 bits (267), Expect = 7.793e-25
Identity = 80/254 (31.50%), Postives = 120/254 (47.24%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNY------NGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLL--VYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKL--EAGGSFEQMLAVAAGWGVTED-----QSSSDILRQVELNIIRNSTCVENM-NAINVPILEDMICTFKGPLGSETICSGDSGGPLMVR----IDGKFTLAGLASFSVTDCSAPFPAVFARISYF 548
            +I+ G  + +HA+PW+ +L Y      + +  CGG+LI++  V+TAAHC+       F    L  V  GEHDL   N+        ++  IIHP Y     +  D+A+L L   VE++  I PICLP L  ++G S          +G+T+        +   + ++ L II N  C     N  NV + E+ +C      G +  C GDSGGPLM          + LAG+ SF        FP V+ R+S +
Sbjct:  202 RIVGGKPSELHAWPWIAALGYRVSGSKDSDFLCGGTLISKRHVVTAAHCV-------FRRSDLSKVRLGEHDLEDENDGAQPRDYGIIKTIIHPDYHPIRFN-NDIAILVLSNDVEFDHRITPICLPDLMKDSGTS-------GFSFGLTKQVRDRLLDAHPFVAEINLEIISNRECSRAFTNFRNVNVTENKLCALDQN-GEKDACQGDSGGPLMTSQGSIAKSNWFLAGVVSFGYRCGVKGFPGVYTRVSEY 439          

BLAST of transmembrane protease serine 9-like vs. L. salmonis genes
Match: EMLSAG00000005882 (supercontig:LSalAtl2s:LSalAtl2s3194:2307:3653:1 gene:EMLSAG00000005882 transcript:EMLSAT00000005882 description:"maker-LSalAtl2s3194-augustus-gene-0.3")

HSP 1 Score: 113.235 bits (282), Expect = 5.766e-28
Identity = 80/249 (32.13%), Postives = 125/249 (50.20%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAAS--LSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTL--GEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPV-GTFDNRMGIAAGWGLTENETNPDVIRQVD----LNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPL-MVLEGTKQVLVGVTSF-GTQNCSDPVSSVYSRVTYFMDWI 821
            KI+ G ++  + FPW  +  +S + G   C GS++    I+TA+HCV     A+F R T+  G HD S+ DE    +    +  I HP++T  +     DIALI LE  + FN  ++P+CLP  S +  TF +    + GWGL +   NP  + Q+     L +I  + C   +  L    +   +IC  +        C GDSGGP+   +E  K + +GV  F G + C D     ++RVT +++WI
Sbjct:   36 KIIGGQEAEPNQFPWVVAILMSSIFGSGRCTGSIVNKQYILTASHCV-----AQFDRFTISAGTHDYSK-DEPHQQIMLATES-IPHPNFTNNMFEYHDDIALIKLEKELEFNDYVRPICLPKYSDMEKTFADETVTSTGWGLIQGSPNPISVPQLHYVNGLRVIKNDVCAQTYGSL----INEDLICIDSSD--HKGVCNGDSGGPMNYEIEDGKYMQIGVADFVGGKTCDDGKPEGFARVTSYLEWI 271          

HSP 2 Score: 103.219 bits (256), Expect = 1.566e-24
Identity = 75/256 (29.30%), Postives = 125/256 (48.83%), Query Frame = 0

Query:  304 FRNKEYLGPSFKIIDGTDAPIHAFPWLVSLNYN---GEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGS-PGDVALLQLDRPVEYNRAIKPICLPKL-EAGGSFEQMLAVAAGWGVTEDQSSSDILRQVE----LNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRI-DGKFTLAGLASF-SVTDCSAPFPAVFARISYF 548
              NKE    S KII G +A  + FPW+V++  +   G   C GS++N+  ++TA+HC+        F +  +  G HD S   +            I HP ++        D+AL++L++ +E+N  ++PICLPK  +   +F      + GWG+ +   +   + Q+     L +I+N  C +   ++   I ED+IC        + +C+GDSGGP+   I DGK+   G+A F     C    P  FAR++ +
Sbjct:   28 IENKE---XSSKIIGGQEAEPNQFPWVVAILMSSIFGSGRCTGSIVNKQYILTASHCVAQ------FDRFTISAGTHDYS--KDEPHQQIMLATESIPHPNFTNNMFEYHDDIALIKLEKELEFNDYVRPICLPKYSDMEKTFADETVTSTGWGLIQGSPNPISVPQLHYVNGLRVIKNDVCAQTYGSL---INEDLICIDSSD--HKGVCNGDSGGPMNYEIEDGKYMQIGVADFVGGKTCDDGKPEGFARVTSY 267          

BLAST of transmembrane protease serine 9-like vs. L. salmonis genes
Match: EMLSAG00000011338 (supercontig:LSalAtl2s:LSalAtl2s781:8981:32530:-1 gene:EMLSAG00000011338 transcript:EMLSAT00000011338 description:"maker-LSalAtl2s781-augustus-gene-0.4")

HSP 1 Score: 116.316 bits (290), Expect = 1.265e-27
Identity = 80/244 (32.79%), Postives = 121/244 (49.59%), Query Frame = 0

Query:  313 SFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEF---TTTRRVLNIIIHP--RYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTED---QSSSDILRQVELNIIRNSTCVE--NMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARIS 546
            S +II G  A     PW V +  +G + CGG L+N   V TAAHC+    +     ++ V  GE D    ++ +    + + +V  I +HP  RY  T     D+A+L+LDRPV Y   I PICLP   +  S      V AGWG T++   ++ + +L +V + II N  C       +I V + ++M C      G    C GDSGGPL++  DG++TL G+ S          P ++ ++S
Sbjct:  231 SKRIIGGKKAKFAELPWQVHIRISG-YQCGGVLLNHLHVATAAHCVHRNKL----DQITVRLGEFDTKNTDKIDEPLDSESFKVTKIRLHPEFRYMLTQPDRYDIAILELDRPVVYKDNILPICLPT--SDFSLTGKTGVVAGWGKTDNSFGKTGTHLLHKVLVPIIENEQCKHWHREKSIAVQLHDEMFCAGHEE-GQMDACLGDSGGPLVINFDGRWTLIGITSAGFGCAVEKQPGIYHKVS 466          

HSP 2 Score: 114.39 bits (285), Expect = 5.094e-27
Identity = 80/280 (28.57%), Postives = 137/280 (48.93%), Query Frame = 0

Query:  552 RRPRSIDKQFPYRSRPYQPTSAFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLS---RPDETSFTVTRRVKRVILHPSYTAFLIGLPG--DIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTEN---ETNPDVIRQVDLNIISLEQCTTEWRLLD-GLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWIS 822
            R+ R +  +   + R        L K ++   +I+ G +++    PW   +   G  + CGG L+ +  + TAAHCV      + I V LGE D     + DE   + + +V ++ LHP +  +++  P   DIA++ L+ PV +   I P+CLPT+    +   + G+ AGWG T+N   +T   ++ +V + II  EQC    R     ++L   M C  +   G    C GDSGGPL++    +  L+G+TS G     +    +Y +V+   +W++
Sbjct:  201 RKMRQLRLETSRKIRLQNDPQCGLTKHNSFSKRIIGGKKAKFAELPWQVHIRISG--YQCGGVLLNHLHVATAAHCVHRNKLDQ-ITVRLGEFDTKNTDKIDEPLDSESFKVTKIRLHPEFR-YMLTQPDRYDIAILELDRPVVYKDNILPICLPTSD--FSLTGKTGVVAGWGKTDNSFGKTGTHLLHKVLVPIIENEQCKHWHREKSIAVQLHDEMFCAGHEE-GQMDACLGDSGGPLVINFDGRWTLIGITSAGFGCAVEKQPGIYHKVSKTAEWLA 473          

BLAST of transmembrane protease serine 9-like vs. SwissProt
Match: gi|61213776|sp|Q5K2P9.1|POLS2_RAT (RecName: Full=Polyserase-2; AltName: Full=Polyserine protease 2; AltName: Full=Serine protease 36; Flags: Precursor)

HSP 1 Score: 134.42 bits (337), Expect = 5.479e-31
Identity = 124/424 (29.25%), Postives = 189/424 (44.58%), Query Frame = 0

Query:  312 PSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYS-------------ATPGSPG------DVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAV-AAGWGVTEDQSSSD---ILRQVELNIIRNSTC---VENMNAINV--PILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYFKRRRRPRSIDKQ----FPYRSRPYQPTSAFLAKESNVLLKIVD-GTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHC-VETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLP 701
            PS +I+ G+DA    +PW VSL+  G H CGGSLI  + V++AAHC       +   +L V  G H  S     E    R V  I+I   YS             A+P   G      D+ALL+L  P +   +++P+CLP+  A   F    A  A GWG  ++        +L++VEL ++  + C          N+   +L  M+C    P G    C GDSGGPL+    G++ LAG+ SF         P VF  ++ ++   R   +  +    FP + +  Q +  +  +E N      + G   R   +PW A ++ V G   C G+L+ +  ++  A C +++P   E  RV L     SRP+E       RV R++ H + +        D+AL+ L   V        VCLP
Sbjct:   47 PSSRIVGGSDAHPGTWPWQVSLHQGGGHICGGSLIAPSWVLSAAHCFVTNGTLEPADELSVLLGVH--SQDGPLEGAHMRSVATILIPDNYSTVELGADLALLRLASPAKLGPSVRGADLALLRLASPAKLGPSVRPVCLPR--ASHLFAHGTACWATGWGDVQEAVPLPLPWVLQEVELRLLGEAACQCLYSRPGPFNLTFQLLPGMLCAGY-PAGRRDTCQGDSGGPLVCEDGGRWFLAGITSFGFGCGRRNRPGVFTAVAPYESWIREHVMGSEPGPVFPSQLQKPQ-SGPWEPREENCTFAQPECGKAPRPGTWPWEAQVT-VPGSTPCYGALVSDRWVLAPASCFLDSPHDFETWRVLLP----SRPEE------ERVARLVAHENASRDFA---SDLALLQLRTRVNLTAAPSAVCLP 450          

HSP 2 Score: 100.523 bits (249), Expect = 1.997e-20
Identity = 80/266 (30.08%), Postives = 118/266 (44.36%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGF---AEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLP------------------GDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETN---PDVIRQVDLNIISLEQCTTEWRLLDGLELT----PAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWI 821
            +IV G+ +    +PW  SL   GG H CGGSLI    +++AAHC  T G    A+ + V LG H    P E +    R V  +++  +Y+   +G                     D+AL+ L  P      ++PVCLP  S +         A GWG  +       P V+++V+L ++    C   +       LT    P M+C    P G   TC+GDSGGPL+  +G +  L G+TSFG          V++ V  +  WI
Sbjct:   50 RIVGGSDAHPGTWPWQVSL-HQGGGHICGGSLIAPSWVLSAAHCFVTNGTLEPADELSVLLGVHSQDGPLEGAHM--RSVATILIPDNYSTVELGADLALLRLASPAKLGPSVRGADLALLRLASPAKLGPSVRPVCLPRASHLFAHGTAC-WATGWGDVQEAVPLPLPWVLQEVELRLLGEAACQCLYSRPGPFNLTFQLLPGMLCA-GYPAGRRDTCQGDSGGPLVCEDGGRWFLAGITSFGFGCGRRNRPGVFTAVAPYESWI 310          

BLAST of transmembrane protease serine 9-like vs. SwissProt
Match: gi|341941729|sp|A6H6T1.2|PRS40_MOUSE (RecName: Full=Serine protease 40; AltName: Full=Testicular serine protease 2; Flags: Precursor)

HSP 1 Score: 128.257 bits (321), Expect = 7.910e-31
Identity = 85/248 (34.27%), Postives = 125/248 (50.40%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQSSSDI---LRQVELNIIRNSTCVENMNAINVP-------ILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPF--PAVFARISYFKR 550
            KI  G  A    +PW  SL   G H CG  LI++N V++AAHC      P  +  +L Y       L + T ++ T  V  +I+H  Y+       D+ LLQL   VEY+  I P C+P+ E     ++    A+GWG   +     +   L + EL I+ N  C +      VP       I +DM+C     + S++IC+GDSGGPL+  ++G + + GL S+S T C  P   P+VFAR+SYF +
Sbjct:   68 KIYGGQIAGAERWPWQASLRLYGRHICGAVLIDKNWVLSAAHCFQRSQEPSDYHVMLGY-----TDLNSPTRYSRTMSVQKVIVHKDYNRFHTQGSDIVLLQLRSSVEYSSHILPACVPE-ENIKIPKEKACWASGWGYLREDVRIPLPNELYEAELIIMSNDQC-KGFFPPPVPGSGRSYYIYDDMVCAADYDM-SKSICAGDSGGPLVCLLEGSWYVVGLTSWSST-CEEPIVSPSVFARVSYFDK 306          

HSP 2 Score: 108.227 bits (269), Expect = 4.761e-24
Identity = 78/254 (30.71%), Postives = 122/254 (48.03%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETN---PDVIRQVDLNIISLEQCT-----------TEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPV--SSVYSRVTYFMDWI 821
            KI  G  +    +PW ASL   G  H CG  LI  + +++AAHC +         V LG  DL+ P  T ++ T  V++VI+H  Y  F      DI L+ L   V ++  I P C+P  + +     +   A+GWG    +     P+ + + +L I+S +QC              + + D       M+C  +  + + S C GDSGGPL+ L      +VG+TS+ +  C +P+   SV++RV+YF  WI
Sbjct:   68 KIYGGQIAGAERWPWQASLRLYGR-HICGAVLIDKNWVLSAAHCFQRSQEPSDYHVMLGYTDLNSP--TRYSRTMSVQKVIVHKDYNRFHTQ-GSDIVLLQLRSSVEYSSHILPACVPEEN-IKIPKEKACWASGWGYLREDVRIPLPNELYEAELIIMSNDQCKGFFPPPVPGSGRSYYIYDD------MVCAADYDM-SKSICAGDSGGPLVCLLEGSWYVVGLTSWSS-TCEEPIVSPSVFARVSYFDKWI 308          

BLAST of transmembrane protease serine 9-like vs. SwissProt
Match: gi|2833277|sp|Q16651.1|PRSS8_HUMAN (RecName: Full=Prostasin; AltName: Full=Channel-activating protease 1; Short=CAP1; AltName: Full=Serine protease 8; Contains: RecName: Full=Prostasin light chain; Contains: RecName: Full=Prostasin heavy chain; Flags: Precursor)

HSP 1 Score: 126.331 bits (316), Expect = 2.104e-30
Identity = 87/260 (33.46%), Postives = 126/260 (48.46%), Query Frame = 0

Query:  310 LGPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQML-AVAAGWGVTEDQSS---SDILRQVELNIIRNSTCVENMNAINVP-----ILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYFKRRRRPRSIDKQ 560
            + P  +I  G+ A    +PW VS+ Y G H CGGSL+++  V++AAHC       + +    V  G H L   +E    +T +  +II HP Y    GS GD+ALLQL RP+ ++R I+PICLP   A  SF   L     GWG      S      L+Q+E+ +I   TC    N    P     + EDM+C      G +  C GDSGGPL   ++G + L G+ S+     +   P V+   S +    + +  + Q
Sbjct:   39 VAPQARITGGSSAVAGQWPWQVSITYEGVHVCGGSLVSEQWVLSAAHCFPSEHHKEAYE---VKLGAHQLDSYSEDAKVSTLK--DIIPHPSY-LQEGSQGDIALLQLSRPITFSRYIRPICLPA--ANASFPNGLHCTVTGWGHVAPSVSLLTPKPLQQLEVPLISRETCNCLYNIDAKPEEPHFVQEDMVCAGYVE-GGKDACQGDSGGPLSCPVEGLWYLTGIVSWGDACGARNRPGVYTLASSYASWIQSKVTELQ 289          

HSP 2 Score: 106.301 bits (264), Expect = 1.603e-23
Identity = 75/251 (29.88%), Postives = 120/251 (47.81%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMG-IAAGWGLTENETN---PDVIRQVDLNIISLEQCTTEWRLLDGLE----LTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWISAALS 826
            +I  G+ +    +PW  S+++ G  H CGGSL+    +++AAHC  +    E   V LG H L    E +   T  +K +I HPSY     G  GDIAL+ L  P+ F++ I+P+CLP  +   +F N +     GWG      +   P  ++Q+++ +IS E C   + +    E    +   M+C      G    C+GDSGGPL         L G+ S+G    +     VY+  + +  WI + ++
Sbjct:   44 RITGGSSAVAGQWPWQVSITYEG-VHVCGGSLVSEQWVLSAAHCFPSEHHKEAYEVKLGAHQLDSYSEDAKVST--LKDIIPHPSY--LQEGSQGDIALLQLSRPITFSRYIRPICLPAAN--ASFPNGLHCTVTGWGHVAPSVSLLTPKPLQQLEVPLISRETCNCLYNIDAKPEEPHFVQEDMVCA-GYVEGGKDACQGDSGGPLSCPVEGLWYLTGIVSWGDACGARNRPGVYTLASSYASWIQSKVT 286          

BLAST of transmembrane protease serine 9-like vs. SwissProt
Match: gi|117618|sp|P07338.1|CTRB1_RAT (RecName: Full=Chymotrypsinogen B; Contains: RecName: Full=Chymotrypsin B chain A; Contains: RecName: Full=Chymotrypsin B chain B; Contains: RecName: Full=Chymotrypsin B chain C; Flags: Precursor)

HSP 1 Score: 124.02 bits (310), Expect = 2.313e-30
Identity = 88/249 (35.34%), Postives = 123/249 (49.40%), Query Frame = 0

Query:  582 LLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPT---NSPVGTFDNRMGIAAGWGLTENET--NPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWISAAL 825
            L +IV+G  +   ++PW  SL    GFH CGGSLI  D ++TAAHC    G      V  GE D    DE +  V  ++ +V  +P +  F +    DI L+ L  P  F++ +  VCLP    + P GT    +    GWG T+      P+ ++Q  L I+S   C   W    G ++T  M C   G  G  S+C GDSGGPL+  +     L G+ S+G+  CS    +VYSRVT  M W+   L
Sbjct:   31 LSRIVNGEDAIPGSWPWQVSLQDKTGFHFCGGSLISEDWVVTAAHC----GVKTSDVVVAGEFDQGS-DEENIQV-LKIAQVFKNPKFNMFTV--RNDITLLKLATPAQFSETVSAVCLPNVDDDFPPGT----VCATTGWGKTKYNALKTPEKLQQAALPIVSEADCKKSW----GSKITDVMTCA--GASGV-SSCMGDSGGPLVCQKDGVWTLAGIVSWGSGVCSTSTPAVYSRVTALMPWVQQIL 260          

HSP 2 Score: 109.768 bits (273), Expect = 1.781e-25
Identity = 73/235 (31.06%), Postives = 125/235 (53.19%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSL-NYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQS--SSDILRQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARIS 546
            +I++G DA   ++PW VSL +  G HFCGGSLI+++ V+TAAHC          +  +V  GE D    ++ E     ++  +  +P+++       D+ LL+L  P +++  +  +CLP ++       + A   GWG T+  +  + + L+Q  L I+  + C ++  +    I + M C     + S   C GDSGGPL+ + DG +TLAG+ S+    CS   PAV++R++
Sbjct:   33 RIVNGEDAIPGSWPWQVSLQDKTGFHFCGGSLISEDWVVTAAHC-------GVKTSDVVVAGEFDQ--GSDEENIQVLKIAQVFKNPKFNMFT-VRNDITLLKLATPAQFSETVSAVCLPNVDDDFPPGTVCA-TTGWGKTKYNALKTPEKLQQAALPIVSEADCKKSWGS---KITDVMTCAGASGVSS---CMGDSGGPLVCQKDGVWTLAGIVSWGSGVCSTSTPAVYSRVT 250          

BLAST of transmembrane protease serine 9-like vs. SwissProt
Match: gi|342187072|sp|Q8K1T0.2|TMPS3_MOUSE (RecName: Full=Transmembrane protease serine 3)

HSP 1 Score: 127.872 bits (320), Expect = 4.328e-30
Identity = 91/245 (37.14%), Postives = 131/245 (53.47%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTEN--ETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTG-STCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWISAAL 825
            +IV G  S L  +PW  SL F  G+H CGGS+I    I+TAAHCV      +   V +G   L      S   +  V+++I H  Y    +G   DIAL+ L +P+ F++ I+P+CLP NS     D ++   +GWG TE+  + +P V+    + +IS + C    R + G  ++P+M+C   G L  G  +C+GDSGGPL+  E     LVG TSFG          VY+R+T F+DWI   L
Sbjct:  216 RIVGGNMSSLTQWPWQVSLQF-QGYHLCGGSIITPLWIVTAAHCVYDLYHPKSWTVQVGLVSLM----DSPVPSHLVEKIIYHSKYKPKRLG--NDIALMKLSEPLTFDETIQPICLP-NSEENFPDGKLCWTSGWGATEDGGDASP-VLNHAAVPLISNKICNH--RDVYGGIISPSMLCA--GYLKGGVDSCQGDSGGPLVCQERRLWKLVGATSFGIGCAEVNKPGVYTRITSFLDWIHEQL 447          

HSP 2 Score: 107.457 bits (267), Expect = 2.866e-23
Identity = 79/242 (32.64%), Postives = 116/242 (47.93%), Query Frame = 0

Query:  311 GPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPG-DVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTED-QSSSDILRQVELNIIRNSTCVENMNAINVPILEDMICT--FKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYF 548
            G S +I+ G  + +  +PW VSL + G H CGGS+I    ++TAAHC     V D +           +SL +      +  V  II H +Y   P   G D+AL++L  P+ ++  I+PICLP  E     +  L   +GWG TED   +S +L    + +I N  C  + +     I   M+C    KG + S   C GDSGGPL+ +    + L G  SF +       P V+ RI+ F
Sbjct:  212 GYSPRIVGGNMSSLTQWPWQVSLQFQGYHLCGGSIITPLWIVTAAHC-----VYDLYHPKSWTVQVGLVSLMDSP--VPSHLVEKIIYHSKYK--PKRLGNDIALMKLSEPLTFDETIQPICLPNSEENFP-DGKLCWTSGWGATEDGGDASPVLNHAAVPLISNKIC-NHRDVYGGIISPSMLCAGYLKGGVDS---CQGDSGGPLVCQERRLWKLVGATSFGIGCAEVNKPGVYTRITSF 439          

BLAST of transmembrane protease serine 9-like vs. nr
Match: gi|555965139|ref|XP_005894629.1| (PREDICTED: ovochymase-1 [Bos mutus])

HSP 1 Score: 232.646 bits (592), Expect = 3.118e-61
Identity = 173/559 (30.95%), Postives = 271/559 (48.48%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQ-MLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINVPIL-EDMICTFKGPLGSETICSGDSGGPLMVRI-DGKFTLAGLASFSV--TDCSAPF--------PAVFARI-----------SYFKRRRR-------------------PRSIDKQFPYRSRPYQPTS-AFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETN-PDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVL-EGTKQVLVGVTSFGTQNCSDPVS-SVYSRVTYFMDWISAAL 825
            +I    ++ +   PW VSL   G HFCGGSLI  + V+TA HC+  G        L V  GE++L   ++ E      V  IIIHP Y+       ++ALL L   V++   ++PIC+P    G  FE+ +  +A+GWG ++E    S+IL++VE+ I+ +  C   +  +N+P L  DM+C    P G +  C  DSGGPL+ R  DG + LAG+ S++   T    PF        P +F+++            Y   R++                   PRS  K  PY      P S  +L++      +I  G ++  H +PW   L F+G  H CGG++I +  I+TAAHCV++     F  +  G+HD  R  + S    RR K +++H  + +  +    DIALI L   + FN  ++PVCLP +S    F + + +  GWG    +      ++Q+ + ++  E C   +       ++  MIC      G     +GDSGGPL+   E    VL G+ S+G   C+ P    V++RV+ F+DWI + +
Sbjct:   30 RISSWRNSTVGGHPWQVSLKLGGHHFCGGSLIQDDLVVTAVHCL-VGLNEKQIKSLTVTAGEYNLFQKDKEEQNIP--VSKIIIHPEYNRLGYMSFNIALLYLKLKVKFGTTVQPICIP--HRGDKFEEGIFCMASGWGKISETSEYSNILQEVEVPIMDDRRCGAMLRGMNLPPLGRDMLCA-SFPDGEKDACQRDSGGPLVCRRDDGVWVLAGITSWAAGCTRVWNPFRNKQRKASPGIFSKVFVLMDFITQSLFYILDRKQPLGGDSLNKIGSTSFPKTNPRSSAKVIPYGVCGIPPFSPQWLSR------RIAGGVEACPHCWPWQVGLRFLGN-HQCGGAIINSIWILTAAHCVQSKNNPLFWTIVAGDHD--RTLKESTEQVRRAKHIVMHEDFDS--LSYDSDIALIQLSSALEFNSVVRPVCLP-HSLEPLFSSEICVVTGWGSANKDGGLASRLQQIQVPVLEREVCERTYYSAHPGGISEKMICAGFAASGGKDVGQGDSGGPLVCKHEKGPFVLYGIVSWGA-GCAQPRKPGVFARVSVFLDWIQSKI 569          

HSP 2 Score: 129.413 bits (324), Expect = 4.715e-27
Identity = 89/267 (33.33%), Postives = 139/267 (52.06%), Query Frame = 0

Query:  290 NPSFSAKGV---FCDSKFRNKEYLGPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLP-KLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINV-PILEDMICTFKGPLGSETICSGDSGGPLMVRID-GKFTLAGLASFSVTDCSAPF-PAVFARISYF 548
            NP  SAK +    C     + ++L  S +I  G +A  H +PW V L + G H CGG++IN   ++TAAHC+     P F++   +  G+HD +L   TE    RR  +I++H  + +      D+AL+QL   +E+N  ++P+CLP  LE    F   + V  GWG   +D   +  L+Q+++ ++    C     + +   I E MIC      G + +  GDSGGPL+ + + G F L G+ S+    C+ P  P VFAR+S F
Sbjct:  306 NPRSSAKVIPYGVCGIPPFSPQWL--SRRIAGGVEACPHCWPWQVGLRFLGNHQCGGAIINSIWILTAAHCVQSKNNPLFWT---IVAGDHDRTLKESTE--QVRRAKHIVMHEDFDSL-SYDSDIALIQLSSALEFNSVVRPVCLPHSLEP--LFSSEICVVTGWGSANKDGGLASRLQQIQVPVLEREVCERTYYSAHPGGISEKMICAGFAASGGKDVGQGDSGGPLVCKHEKGPFVLYGIVSWG-AGCAQPRKPGVFARVSVF 561          

BLAST of transmembrane protease serine 9-like vs. nr
Match: gi|954576308|ref|XP_014612568.1| (PREDICTED: transmembrane protease serine 9-like [Polistes canadensis])

HSP 1 Score: 226.098 bits (575), Expect = 1.005e-60
Identity = 167/542 (30.81%), Postives = 268/542 (49.45%), Query Frame = 0

Query:  299 FCDSKFRNKEYLGPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQSS-SDILRQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMV-RIDGKFTLAGLASFSVTDCSAP-FPAVFARISYFKRRRRPRSIDKQFPYRSRPYQPT-------SAFLAKESNVLL-------KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTE-NETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEG-TKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWI 821
            +C    +N E      +I+ G    ++  PW+V L+Y  + +CGGSLIN   V+TAAHC+        +  + V FGEHD  +    E   TR V+ ++      +      D+ALLQL+  V +   I+PICLP  +    +    A+AAGWG  +++   S +L++VE+ ++    C  N +     I ++M+C    P G +  C GDSGGPL+  R D ++ + G+ S+    C+ P +P V+ RI+ +       S D  F   ++            S FL   S+  L       +I+ G  S+ H FPW   L   G  H CGG+LI +  ++TA HCV     A  I V LG HD+S   E  + V   + + ILH ++T+ +I    DIALI L++PV ++  ++P+CLP       +  +     GWG+   N T    +R+ +L I+SL  C        G  LT +++C +         C+GDSGGP+ + +   K  ++G+ S+G    +  V  +Y ++T ++ WI
Sbjct:   51 YCSCGLQNDES-----RIVGGQTTRMNELPWMVRLSYLNKFYCGGSLINDRYVLTAAHCVKGFLFRFMWFMIKVTFGEHDRCIDKSLE---TRYVVRVLTGD--FSFLNFDNDIALLQLNDRVPFGDTIRPICLPN-KKENEYIGSKAIAAGWGTLQEEGKPSCLLQKVEVPVMSLQDC-RNTSYSPRMISDNMLCA-GYPDGKKDSCQGDSGGPLITEREDKRYEIIGIVSWG-NGCARPGYPGVYTRITNYLDWILNNSKDGCFCDNNKSSLKMFINNCIISHFLLTMSDCGLSSMGLSNRIIGGKISKPHDFPWIVILFKRGAIH-CGGALINDRYVLTAGHCVRWTK-ANDITVGLGIHDVSDL-EDGYVVA--ISKTILHENFTSKVIHDTNDIALIKLQEPVEYDNHLQPICLPNKDD--DYSGKTAKVTGWGIVAVNGTTSKFLRETNLIILSLNSCKNT---SIGNYLTNSVMCAYK---DNTDACQGDSGGPIFIEKTIAKYEIIGIVSWGLGCANKGVPGIYVKITDYLSWI 565          

BLAST of transmembrane protease serine 9-like vs. nr
Match: gi|973216833|ref|XP_015194903.1| (PREDICTED: transmembrane protease serine 9-like [Lepisosteus oculatus])

HSP 1 Score: 234.187 bits (596), Expect = 1.807e-60
Identity = 175/536 (32.65%), Postives = 264/536 (49.25%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQSSS--DILRQVELNII--RNSTCVENMNAINVPILEDMICTFKGPL-GSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAP-FPAVFARISYFKR----------------RRRPRSIDKQF---PYRSRPYQPTSAFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLT-ENETNP--DVIRQVDLNIISLEQCTTEWRLLDGL-ELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWI 821
            +I+ G  A   ++PW  SL+Y G+H CGGSLINQ  V+TAAHC+   + P       VY G      ++  E   +R V  II+HP Y++      D+AL++L  PV +   I+PICL    +   +        GWG T +  ++    L++V+L II  R   C+ ++      +  +M+C   G L G +  C GDSGGPL+ +    +  AG+ SF    C+ P FP V+ R+S +K                        DK F   P++  P  P+    A  S    +IV G  +    +PW  S+ +  G++ CGGSLI    ++TAA CV +   +E ++V LG    + P+     ++++VK+VILHP Y++       DIAL+ L  PV F   I+P+CL  NS    +       AGWG T E  + P    +++V L II   QC   + L  G   +  +MIC    P G+  TCRGD GGPL+  +G+  V  G+   G       +  +Y+RV+ + DWI
Sbjct:  336 RIVGGNPAQEGSWPWQASLHYYGQHMCGGSLINQQWVLTAAHCI---SDPLNTYPWTVYLGRLTQFSSSPNEL--SRSVQQIIVHPAYNSVT-EDNDIALMRLSSPVSFTSYIQPICLAD-NSSSFYNGTSCWVTGWGYTAEDGATLPTTLQEVQLPIIGNRQCGCLNDVVFGANSVTGNMLC--AGVLQGGKDSCQGDSGGPLVCKQGPSWVQAGVVSFG-EGCAQPNFPGVYTRVSQYKDWINTQVGLATPGFVTFSSNSTDPDKNFTCSPFKITPVAPSECGKAPLST---RIVGGVSAPEGYWPWQVSIYYF-GYYRCGGSLINQQWVLTAATCVYSAWTSE-LKVYLGRQTQAGPNPQE--LSKKVKQVILHPGYSSS----NNDIALLELRSPVTFTNYIQPICLAANSS-SFYTGTSCWVAGWGFTSEFMSLPGNQTLQEVQLPIIGNRQCGCLYDLNYGPGSVNDSMICAGQ-PNGSKDTCRGDEGGPLICKQGSSWVQAGIVRPGEGCGQTGLPGLYTRVSQYEDWI 848          

HSP 2 Score: 227.254 bits (578), Expect = 3.837e-58
Identity = 176/559 (31.48%), Postives = 267/559 (47.76%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQSSS--DILRQVELNII--RNSTCVENMNAINVPILEDMICTFKGPL-GSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYFKRRRRPR--------------SIDKQFPYRSRPYQPTSAFLAKESNVLL----------------------KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLT--ENETNPDVIRQVDLNIISLEQCTTEWRLLDGLE-LTPAMICTFNGPL-GTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDP-VSSVYSRVTYFMDWISAALSV 827
            +I+ G  A   ++PW  SL  NG H CGG+LIN   V+TAAHC  FG+ P   S+  VY G  D      +  T + +VL II HP Y ++  +  D+AL++L+  V +N  I+P+CL    +   +        GWG T +  ++    L++V+L II  R   C+ ++      +  +MIC   G L G +  C GDSGGPL+ +    +  AG+ SF         P V+ R+S +K     +                D    +   P   T A L       +                      +IV G  ++  ++PW ASL + G  H CGGSLI    ++TAAHC+  P       V LG   L++   +   ++R V+++I+HP+Y +  +    DIAL+ L  PV F   I+P+CL  NS    ++       GWG T  +  T P  +++V L II   QC     ++ G   +T  M+C   G L G   +C+GDSGGPL+  +G   V  GV SFG + C+ P    VY+RV+ + DWI+  + +
Sbjct:   36 RIVGGQSAQPGSWPWQASLRLNGGHTCGGTLINSQWVLTAAHC--FGS-PITPSQWTVYLGVTD---TGSSTNTVSSKVLQIIRHPNYDSSTNN-NDIALMKLNSSVSFNNYIQPVCLADTSSS-FYNGTSCWVTGWGDTVEGGTTLPSTLQEVQLPIIGNRQCGCLNDVVFGANSVTGNMIC--AGVLEGGKDSCQGDSGGPLVCKQGSAWVQAGIVSFGYGCARPSLPGVYTRVSQYKDWINGQVGSGDTGFVTFTSSGTDADSSFTCSPTVTTRAPLTTPVTPSITYPTPPPTPIAPNECGKVPLNPRIVGGNPAQEGSWPWQASLHYYGQ-HMCGGSLINQQWVLTAAHCISDPLNTYPWTVYLGR--LTQFSSSPNELSRSVQQIIVHPAYNS--VTEDNDIALMRLSSPVSFTSYIQPICLADNSS-SFYNGTSCWVTGWGYTAEDGATLPTTLQEVQLPIIGNRQCGCLNDVVFGANSVTGNMLCA--GVLQGGKDSCQGDSGGPLVCKQGPSWVQAGVVSFG-EGCAQPNFPGVYTRVSQYKDWINTQVGL 575          

HSP 3 Score: 221.09 bits (562), Expect = 4.316e-56
Identity = 164/535 (30.65%), Postives = 258/535 (48.22%), Query Frame = 0

Query:  313 SFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQSS---SDILRQVELNII--RNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYFK---RRRRPRSIDKQFPYRSRPYQPTSAFLAK-------------ESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDL--SRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENET-NPDVIRQVDLNIISLEQCTTEWRLLDGLE-LTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWIS 822
            S +I+ G  AP   +PW VS+ Y G + CGGSLINQ  V+TAA C+       + S+L VY G    +  N  E   +++V  +I+HP YS+   S  D+ALL+L  PV +   I+PICL    +   +       AGWG T +  S   +  L++V+L II  R   C+ ++N     + + MIC  + P GS+  C GD GGPL+ +    +  AG+            P ++ R+S ++    +    +      + S    P   F                 + +  +I  G  ++   +PW ASL +  G++ CGGSLI +  +++AA CV        + V LG  +   S P E    V+R+V RVILHP + +       DIAL+ L+ PV F   I+P+CL   S   +      +  GWG T++ +    ++++  + I+   QC   +    G+  +T  MIC    P G  +TC GDSGGPL+  +G+  +  GV  +        +  VY+RV+ + DWI+
Sbjct:  615 STRIVGGVSAPEGYWPWQVSIYYFGYYRCGGSLINQQWVLTAATCV----YSAWTSELKVYLGRQTQAGPNPQEL--SKKVKQVILHPGYSS---SNNDIALLELRSPVTFTNYIQPICL-AANSSSFYTGTSCWVAGWGFTSEFMSLPGNQTLQEVQLPIIGNRQCGCLYDLNYGPGSVNDSMICAGQ-PNGSKDTCRGDEGGPLICKQGSSWVQAGIVRPGEGCGQTGLPGLYTRVSQYEDWIYQMVGMATPGFVTFSSNGSDPERNFTCSPPSSTPISSSECGRAPLRTRIAGGQSAQEGYWPWQASLHY-SGYYMCGGSLINHQWVLSAAGCVYRISDNRQLTVYLGRQNQMNSNPSE----VSRKVTRVILHPDFNSTT--FTNDIALLRLQTPVNFTNYIQPICLADKSSSFSTGTSCWV-TGWGNTDDYSFYAQILQEAQVPIMGNRQCGCLYAEAFGVNSITENMICA-GVPDGGNATCLGDSGGPLVCKQGSAWIQAGVVKYRDGCTPSSLPGVYTRVSQYQDWIN 1129          

HSP 4 Score: 180.259 bits (456), Expect = 6.882e-43
Identity = 136/426 (31.92%), Postives = 203/426 (47.65%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQS-SSDILRQVELNIIRNSTC-VENMNAINV-PILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYFKR----------------RRRPRSIDKQFPYRSRPYQPTSAFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGI-AAGWG 720
            +I  G  A    +PW  SL+Y+G + CGGSLIN   V++AA C+    + D   +L VY G  +   +N +E +  R+V  +I+HP +++T  +  D+ALL+L  PV +   I+PICL    +  S      V  GWG T+D S  + IL++ ++ I+ N  C      A  V  I E+MIC    P G    C GDSGGPL+ +    +  AG+  +      +  P V+ R+S ++                        D  F   +    P +     ++ +  +IV G  +    +PW ASL   G  H CGGSLI +  ++TAAHCV        + V LG    S+    +  V+R V  VI HP Y+ F      D+AL+ L  PV F   I+P+CL +N  + +F N       GWG
Sbjct:  896 RIAGGQSAQEGYWPWQASLHYSGYYMCGGSLINHQWVLSAAGCVY--RISDN-RQLTVYLGRQNQMNSNPSEVS--RKVTRVILHPDFNSTTFT-NDIALLRLQTPVNFTNYIQPICLADKSSSFSTGTSCWVT-GWGNTDDYSFYAQILQEAQVPIMGNRQCGCLYAEAFGVNSITENMICAGV-PDGGNATCLGDSGGPLVCKQGSAWIQAGVVKYRDGCTPSSLPGVYTRVSQYQDWINELVWSATPGLLTFTSNGTDTDSNFTCSALGTTPVTPSECGKAPLSPRIVGGDSAPEGYWPWQASLHHYGQ-HICGGSLINHQWVLTAAHCVNGISDGRQLTVYLGRQ--SQLGSNTHEVSRMVTMVIPHPGYSNF----DKDVALLRLNKPVSFTAYIQPICLASN--ISSFQNSTQCWVTGWG 1304          

BLAST of transmembrane protease serine 9-like vs. nr
Match: gi|742186913|ref|XP_010854338.1| (PREDICTED: ovochymase-1 [Bison bison bison])

HSP 1 Score: 234.187 bits (596), Expect = 1.924e-60
Identity = 170/548 (31.02%), Postives = 268/548 (48.91%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQ-MLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINVPIL-EDMICTFKGPLGSETICSGDSGGPLMVRI-DGKFTLAGLASFSV--TDCSAPF--------PAVFARISYFKR-------------------RRRPRSIDKQFPYRSRPYQPTS-AFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETN-PDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVL-EGTKQVLVGVTSFGTQNCSDPVS-SVYSRVTYFMDWISAAL 825
            +I    ++ +   PW VSL   G HFCGGSLI  + V+TA HC+  G        L V  GE++L   ++ E      V  IIIHP Y+       ++ALL L   V++   ++PIC+P    G  FE+ +  +A+GWG ++E    S+IL++VE+ I+ +  C   +  +N+P L  DM+C    P G +  C  DSGGPL+ R  DG + LAG+ S++   T    PF        P +F+++                        +  PRS  K  PY      P S  +L++      +I  G ++  H +PW   L F+G  H CGG++I +  I+TAAHCV++     F  +  G+HD +  + T     RR K +++H  + +  +    DIALI L   + FN  ++PVCLP +S    F + + +  GWG    +      ++Q+ + ++  E C   +       ++  MIC      G     +GDSGGPL+   E    VL G+ S+G   C+ P    V++RV+ F+DWI + +
Sbjct:   58 RISSWRNSTVGGHPWQVSLKLGGHHFCGGSLIQDDLVVTAVHCL-IGLNEKQIKSLTVTAGEYNLFQKDKEEQNIP--VSKIIIHPEYNRLGYMSFNIALLYLKLKVKFGTTVQPICIP--HRGDKFEEGIFCMASGWGKISETSEYSNILQEVEVPIMDDRRCGAMLRGMNLPPLGRDMLCA-SFPDGEKDACQRDSGGPLVCRRDDGVWVLAGITSWAAGCTRVWNPFRNKQRKASPGIFSKVFVLMDFITQTMTDSLNKIGSTSFPKTNPRSSAKVIPYGFCGIPPFSPQWLSR------RIAGGVEACPHCWPWQVGLRFLGN-HQCGGAIINSIWILTAAHCVQSKNNPLFWTIVAGDHDRTLMESTE--QVRRAKHIVMHEDFNS--LSYDSDIALIQLSSALEFNSVVRPVCLP-HSLEPLFSSEICVVTGWGSANKDGGLASRLQQIQVPVLEREVCERTYYSAHPGGISEKMICAGFAASGGKDVGQGDSGGPLVCKHEKGPFVLYGIVSWGA-GCAQPRKPGVFARVSVFLDWIQSKI 586          

HSP 2 Score: 132.109 bits (331), Expect = 1.113e-27
Identity = 90/267 (33.71%), Postives = 141/267 (52.81%), Query Frame = 0

Query:  290 NPSFSAKGV---FCDSKFRNKEYLGPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLP-KLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINV-PILEDMICTFKGPLGSETICSGDSGGPLMVRID-GKFTLAGLASFSVTDCSAPF-PAVFARISYF 548
            NP  SAK +   FC     + ++L  S +I  G +A  H +PW V L + G H CGG++IN   ++TAAHC+     P F++   +  G+HD +L   TE    RR  +I++H  +++      D+AL+QL   +E+N  ++P+CLP  LE    F   + V  GWG   +D   +  L+Q+++ ++    C     + +   I E MIC      G + +  GDSGGPL+ + + G F L G+ S+    C+ P  P VFAR+S F
Sbjct:  323 NPRSSAKVIPYGFCGIPPFSPQWL--SRRIAGGVEACPHCWPWQVGLRFLGNHQCGGAIINSIWILTAAHCVQSKNNPLFWT---IVAGDHDRTLMESTE--QVRRAKHIVMHEDFNSL-SYDSDIALIQLSSALEFNSVVRPVCLPHSLEP--LFSSEICVVTGWGSANKDGGLASRLQQIQVPVLEREVCERTYYSAHPGGISEKMICAGFAASGGKDVGQGDSGGPLVCKHEKGPFVLYGIVSWG-AGCAQPRKPGVFARVSVF 578          

HSP 3 Score: 120.939 bits (302), Expect = 3.554e-24
Identity = 80/246 (32.52%), Postives = 124/246 (50.41%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVT----LGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETN--PDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWISA 823
            ++V G  + + ++PW  SL    G H CGG+LI    ++TAAHC        F  VT    +G   L     +       VK V +HPS+T F      D++L+ LE PV   + +  +CLP         ++  + AGWG+TE   +  P  ++Q  + +IS   C + W    GLE+    IC   G     S+C GDSGGPL   EG +  L+G+ S+G+ NC     +V++R++ + DWI++
Sbjct: 1066 RVVGGRAAAVMSWPWLVSLQH-QGHHYCGGALIGRRWVLTAAHC-------NFSTVTDYLVIGRSYLGNIRNSDLI---PVKAVYIHPSFTQFPPN--DDLSLLHLEKPVELGEFVSTICLPGKDDKINLLSKC-LTAGWGITEPHQDEFPKTVQQAKVPLISSISCRSYW----GLEIKNTNIC---GGASGSSSCMGDSGGPLQCGEGGQYKLIGIVSWGSSNCHPAAPTVFTRISAYTDWITS 1290          

HSP 4 Score: 118.242 bits (295), Expect = 2.594e-23
Identity = 76/236 (32.20%), Postives = 122/236 (51.69%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQSSS--DILRQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYF 548
            +++ G  A + ++PWLVSL + G H+CGG+LI +  V+TAAHC +F  V D+     +  G   L     ++    + V    IHP ++  P +  D++LL L++PVE    +  ICLP  +   +      + AGWG+TE         ++Q ++ +I + +C    +   + I    IC   G  GS + C GDSGGPL     G++ L G+ S+  ++C    P VF RIS +
Sbjct: 1066 RVVGGRAAAVMSWPWLVSLQHQGHHYCGGALIGRRWVLTAAHC-NFSTVTDY-----LVIGRSYLGNIRNSDLIPVKAVY---IHPSFTQFPPN-DDLSLLHLEKPVELGEFVSTICLPGKDDKINLLSK-CLTAGWGITEPHQDEFPKTVQQAKVPLISSISC---RSYWGLEIKNTNIC--GGASGSSS-CMGDSGGPLQCGEGGQYKLIGIVSWGSSNCHPAAPTVFTRISAY 1284          

BLAST of transmembrane protease serine 9-like vs. nr
Match: gi|982926575|ref|XP_015326695.1| (PREDICTED: ovochymase-1 [Bos taurus] >gi|982982157|ref|XP_015318917.1| PREDICTED: ovochymase-1 [Bos taurus])

HSP 1 Score: 233.032 bits (593), Expect = 4.365e-60
Identity = 170/549 (30.97%), Postives = 269/549 (49.00%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQ-MLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINVPIL-EDMICTFKGPLGSETICSGDSGGPLMVRI-DGKFTLAGLASFSV--TDCSAPF--------PAVFARISYFKR-------------------RRRPRSIDKQFPYRSRPYQPTS-AFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETN-PDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQ--VLVGVTSFGTQNCSDPVS-SVYSRVTYFMDWISAAL 825
            +I    ++ +   PW VSL   G HFCGGSLI  + V+TA HC+  G        L V  GE++L   ++ E      V  IIIHP Y+       ++ALL L   V++   ++PIC+P    G  FE+ +  +A+GWG ++E    S+IL++VE+ I+ +  C   +  +N+P L  DM+C    P G +  C  DSGGPL+ R  DG + LAG+ S++   T    PF        P +F+++                        +  PRS  K  PY      P S  +L++      +I  G ++  H +PW   L F+G  H CGG++I +  I+TAAHCV++     F  +  G+HD++  + T     RR K +++H  + +  +    DIALI L   + FN  ++PVCLP +S    F + + +  GWG    +      ++Q+ + ++  E C   +       ++  MIC      G     +GDSGG L+V +  K   VL G+ S+G   C  P    V++RV+ F+DWI + +
Sbjct:   30 RISSWRNSTVGGHPWQVSLKLGGHHFCGGSLIQDDLVVTAVHCL-IGLNEKQIKSLTVTAGEYNLFQKDKEEQNIP--VSKIIIHPEYNRLGYMSFNIALLYLKLKVKFGTTVQPICIP--HRGDKFEEGIFCMASGWGKISETSEYSNILQEVEVPIMDDRRCGAMLRGMNLPPLGRDMLCA-SFPDGEKDACQRDSGGPLVCRRDDGVWVLAGITSWAAGCTRVWNPFRNKQRKASPGIFSKVFVLMDFITQTMTDSLNKIGSTSFPKTNPRSSAKVIPYGVCGIPPFSPQWLSR------RIAGGVEACPHCWPWQVGLRFLGN-HQCGGAIINSIWILTAAHCVQSKNNPLFWTIVAGDHDITLKESTE--QVRRAKHIVMHEDFDS--LSYDSDIALIQLSSALEFNSVVRPVCLP-HSLEPLFSSEICVVTGWGSANKDGGLASRLQQIQVPVLEREVCERTYYSAHPGGISEKMICAGFAASGEKDVGQGDSGG-LLVCKHEKGPFVLYGIVSWGA-GCDQPRKPGVFARVSVFLDWIQSKI 558          

HSP 2 Score: 126.716 bits (317), Expect = 6.761e-26
Identity = 88/267 (32.96%), Postives = 138/267 (51.69%), Query Frame = 0

Query:  290 NPSFSAKGV---FCDSKFRNKEYLGPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLP-KLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINV-PILEDMICTFKGPLGSETICSGDSGGPLMVRID-GKFTLAGLASFSVTDCSAPF-PAVFARISYF 548
            NP  SAK +    C     + ++L  S +I  G +A  H +PW V L + G H CGG++IN   ++TAAHC+     P F++   +  G+HD++L   TE    RR  +I++H  + +      D+AL+QL   +E+N  ++P+CLP  LE    F   + V  GWG   +D   +  L+Q+++ ++    C     + +   I E MIC      G + +  GDSGG L+ + + G F L G+ S+    C  P  P VFAR+S F
Sbjct:  295 NPRSSAKVIPYGVCGIPPFSPQWL--SRRIAGGVEACPHCWPWQVGLRFLGNHQCGGAIINSIWILTAAHCVQSKNNPLFWT---IVAGDHDITLKESTE--QVRRAKHIVMHEDFDSL-SYDSDIALIQLSSALEFNSVVRPVCLPHSLEP--LFSSEICVVTGWGSANKDGGLASRLQQIQVPVLEREVCERTYYSAHPGGISEKMICAGFAASGEKDVGQGDSGGLLVCKHEKGPFVLYGIVSWG-AGCDQPRKPGVFARVSVF 550          

HSP 3 Score: 120.939 bits (302), Expect = 3.417e-24
Identity = 80/246 (32.52%), Postives = 124/246 (50.41%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVT----LGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETN--PDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWISA 823
            ++V G  + + ++PW  SL    G H CGG+LI    ++TAAHC        F  VT    +G   L     +       VK V +HPS+T F      D++L+ LE PV   + +  +CLP         ++  + AGWG+TE   +  P  ++Q  + +IS   C + W    GLE+    IC   G     S+C GDSGGPL   EG +  L+G+ S+G+ NC     +V++R++ + DWI++
Sbjct: 1038 RVVGGRAAAVMSWPWLVSLQH-QGHHYCGGALIGRRWVLTAAHC-------NFSTVTDYLVIGRSYLGNIRNSDLI---PVKAVYIHPSFTQFPPN--DDLSLLHLEKPVELGEFVSTICLPGKDDKINLLSKC-LTAGWGITEPHQDEFPKTVQQAKVPLISSISCRSYW----GLEIKNTNIC---GGASGSSSCMGDSGGPLQCGEGGQYKLIGIVSWGSSNCHPAAPTVFTRISAYTDWITS 1262          

HSP 4 Score: 118.242 bits (295), Expect = 2.231e-23
Identity = 76/236 (32.20%), Postives = 122/236 (51.69%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQSSS--DILRQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYF 548
            +++ G  A + ++PWLVSL + G H+CGG+LI +  V+TAAHC +F  V D+     +  G   L     ++    + V    IHP ++  P +  D++LL L++PVE    +  ICLP  +   +      + AGWG+TE         ++Q ++ +I + +C    +   + I    IC   G  GS + C GDSGGPL     G++ L G+ S+  ++C    P VF RIS +
Sbjct: 1038 RVVGGRAAAVMSWPWLVSLQHQGHHYCGGALIGRRWVLTAAHC-NFSTVTDY-----LVIGRSYLGNIRNSDLIPVKAVY---IHPSFTQFPPN-DDLSLLHLEKPVELGEFVSTICLPGKDDKINLLSK-CLTAGWGITEPHQDEFPKTVQQAKVPLISSISC---RSYWGLEIKNTNIC--GGASGSSS-CMGDSGGPLQCGEGGQYKLIGIVSWGSSNCHPAAPTVFTRISAY 1256          

BLAST of transmembrane protease serine 9-like vs. nr
Match: gi|1131278537|ref|XP_019816954.1| (PREDICTED: ovochymase-1-like [Bos indicus])

HSP 1 Score: 232.261 bits (591), Expect = 6.915e-60
Identity = 169/548 (30.84%), Postives = 267/548 (48.72%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQ-MLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINVPIL-EDMICTFKGPLGSETICSGDSGGPLMVRI-DGKFTLAGLASFSV--TDCSAPF--------PAVFARISYFKR-------------------RRRPRSIDKQFPYRSRPYQPTS-AFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETN-PDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVL-EGTKQVLVGVTSFGTQNCSDPVS-SVYSRVTYFMDWISAAL 825
            +I    ++ +   PW +SL   G HFCGGSLI  + V+TA HC+  G        L V  GE++L   ++ E      V  IIIHP Y+       ++ALL L   V++   ++PIC+P    G  FE+ +  +A+GWG ++E    S+IL++VE+ I  +  C   +  +N+P L  DM+C    P G +  C  DSGGPL+ R  DG + LAG+ S++   T    PF        P +F+++                        +  P+S  K  PY      P S  +L++      +I  G ++  H +PW   L F+G  H CGG++I +  I+TAAHCV++     F  +  G+HD  R  + S    RR K +++H  + +  +    DIALI L   + FN  ++PVCLP +S    F + + +  GWG    +      ++Q+ + ++  E C   +       ++  MIC      G     +GDSGGPL+   E    VL G+ S+G   C+ P    V++RV+ F+DWI + +
Sbjct:   30 RISSWRNSTVGGHPWQISLKLGGHHFCGGSLIQDDLVVTAVHCL-IGLNEKQIKSLTVTAGEYNLFQKDKEEQNIP--VSKIIIHPEYNRLGYMSFNIALLYLKLKVKFGTTVQPICIP--HRGDKFEEGIFCMASGWGKISETSEYSNILQEVEVPIXDDRRCGAMLRGMNLPPLGRDMLCA-SFPDGEKDACQRDSGGPLVCRRDDGVWVLAGITSWAAGCTRVWNPFRNKQRKASPGIFSKVFVLMDFITQTMTDSLNKIGSTSFPKTNPKSSAKVIPYGVCGIPPFSPQWLSR------RIAGGVEACPHCWPWQVGLRFLGN-HQCGGAIINSIWILTAAHCVQSKNNPLFWTIVAGDHD--RTLKESTEQVRRAKHIVMHEDFDS--LSYDSDIALIQLSSALEFNSVVRPVCLP-HSLEPLFSSEICVVTGWGSANKDGGLASRLQQIQVPVLEREVCERTYYSAHPGGISEKMICAGFAASGEKDVGQGDSGGPLVCKHEKGPFVLYGIVSWGA-GCAQPRKPGVFARVSVFLDWIQSKI 558          

HSP 2 Score: 129.413 bits (324), Expect = 7.475e-27
Identity = 89/267 (33.33%), Postives = 139/267 (52.06%), Query Frame = 0

Query:  290 NPSFSAKGV---FCDSKFRNKEYLGPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLP-KLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINV-PILEDMICTFKGPLGSETICSGDSGGPLMVRID-GKFTLAGLASFSVTDCSAPF-PAVFARISYF 548
            NP  SAK +    C     + ++L  S +I  G +A  H +PW V L + G H CGG++IN   ++TAAHC+     P F++   +  G+HD +L   TE    RR  +I++H  + +      D+AL+QL   +E+N  ++P+CLP  LE    F   + V  GWG   +D   +  L+Q+++ ++    C     + +   I E MIC      G + +  GDSGGPL+ + + G F L G+ S+    C+ P  P VFAR+S F
Sbjct:  295 NPKSSAKVIPYGVCGIPPFSPQWL--SRRIAGGVEACPHCWPWQVGLRFLGNHQCGGAIINSIWILTAAHCVQSKNNPLFWT---IVAGDHDRTLKESTE--QVRRAKHIVMHEDFDSL-SYDSDIALIQLSSALEFNSVVRPVCLPHSLEP--LFSSEICVVTGWGSANKDGGLASRLQQIQVPVLEREVCERTYYSAHPGGISEKMICAGFAASGEKDVGQGDSGGPLVCKHEKGPFVLYGIVSWG-AGCAQPRKPGVFARVSVF 550          

HSP 3 Score: 120.939 bits (302), Expect = 3.756e-24
Identity = 80/246 (32.52%), Postives = 124/246 (50.41%), Query Frame = 0

Query:  584 KIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVT----LGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETN--PDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDPVSSVYSRVTYFMDWISA 823
            ++V G  + + ++PW  SL    G H CGG+LI    ++TAAHC        F  VT    +G   L     +       VK V +HPS+T F      D++L+ LE PV   + +  +CLP         ++  + AGWG+TE   +  P  ++Q  + +IS   C + W    GLE+    IC   G     S+C GDSGGPL   EG +  L+G+ S+G+ NC     +V++R++ + DWI++
Sbjct: 1038 RVVGGRAAAVMSWPWLVSLQH-QGHHYCGGALIGRRWVLTAAHC-------NFSTVTDYLVIGRSYLGNIRNSDLI---PVKAVYIHPSFTQFPPN--DDLSLLHLEKPVELGEFVSTICLPGKDDKINLLSKC-LTAGWGITEPHQDEFPKTVQQAKVPLISSISCRSYW----GLEIKNTNIC---GGASGSSSCMGDSGGPLQCGEGGQYKLIGIVSWGSSNCHPAAPTVFTRISAYTDWITS 1262          

HSP 4 Score: 118.242 bits (295), Expect = 2.474e-23
Identity = 76/236 (32.20%), Postives = 122/236 (51.69%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQSSS--DILRQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYF 548
            +++ G  A + ++PWLVSL + G H+CGG+LI +  V+TAAHC +F  V D+     +  G   L     ++    + V    IHP ++  P +  D++LL L++PVE    +  ICLP  +   +      + AGWG+TE         ++Q ++ +I + +C    +   + I    IC   G  GS + C GDSGGPL     G++ L G+ S+  ++C    P VF RIS +
Sbjct: 1038 RVVGGRAAAVMSWPWLVSLQHQGHHYCGGALIGRRWVLTAAHC-NFSTVTDY-----LVIGRSYLGNIRNSDLIPVKAVY---IHPSFTQFPPN-DDLSLLHLEKPVELGEFVSTICLPGKDDKINLLSK-CLTAGWGITEPHQDEFPKTVQQAKVPLISSISC---RSYWGLEIKNTNIC--GGASGSSS-CMGDSGGPLQCGEGGQYKLIGIVSWGSSNCHPAAPTVFTRISAY 1256          

BLAST of transmembrane protease serine 9-like vs. nr
Match: gi|1199369296|ref|XP_021195380.1| (polyserase-2-like [Helicoverpa armigera])

HSP 1 Score: 222.631 bits (566), Expect = 7.475e-60
Identity = 161/522 (30.84%), Postives = 253/522 (48.47%), Query Frame = 0

Query:  311 GPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATP-GSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTED--QSSSDILRQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMV----RIDGKFTLAGLASFSVTDCSAPFPAVFARISYFKRRRRPRSIDKQFPYRSRPYQPTSAFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENE-TNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFN-GPLGTGSTCRGDSGGPLMVLEGT-KQVLVGVTSFGTQNCSDP-VSSVYSRVTYFMDWI 821
            G + +I+ GT    HA+PWLVSL    +  CGG++I    ++TA HC+ FG     +  L VY G HD     +T + T   V N + HP +++       D+A+L LD+ +++   ++PICLP  E    F  +    AGWG T     +SS  L + ++ I+ +  C ++    +  + + M+C +   LG +  C GDSGGPL        + K+   G+ S+ + DC+ P                       +P   RP          +  V ++IV G ++  H+FPW  ++      H CGG++I +  +++A HC +   F + + V +G   L   D+      R +  V++H ++T+  +    DIA+  L  PV FN  I P+CLP   P   F NR+G   GWG    E ++  V+ +  L I+S E C           L P M+C F+ G  G    C+GDSGGPL+V +   K V  GV S+G   C+DP    VY++V  ++DWI
Sbjct:   64 GHNRRIVGGTTVEPHAYPWLVSLMLGPKLHCGGAIITDLHILTAGHCITFGV---HYRDLTVYVGMHDRL---DTSYVTM-HVENGVKHPHFTSNAVRDINDIAVLTLDKKLKFTENVRPICLP--EDSMDFRNLPLTVAGWGKTRQGALTSSRYLLETKVQIVDSDKCKKSAIYRDNLVGDTMMCAYS--LGKDA-CQGDSGGPLFSTHRRSYNKKWYQVGIVSWGI-DCAMP----------------------DYPECGRP---------SDKVVSMRIVGGRRAEPHSFPWTVAILKNERMH-CGGAMITDKHVLSAGHCFKWDNF-KTMEVLIG---LDNMDDLKNVEKRNISNVVIHEAFTSTAVRDENDIAIATLNHPVAFNDNIVPICLP--EPAEDFTNRIGTIVGWGRVGVEKSSSKVLLKASLRILSDEDCMNSQL---AQHLKPTMMCAFSKGKDG----CQGDSGGPLLVFQPEGKYVQAGVVSWGI-GCADPRYPGVYTKVNNYIDWI 526          

BLAST of transmembrane protease serine 9-like vs. nr
Match: gi|1316196792|ref|XP_023244123.1| (transmembrane protease serine 9-like [Centruroides sculpturatus])

HSP 1 Score: 222.246 bits (565), Expect = 1.681e-59
Identity = 169/553 (30.56%), Postives = 260/553 (47.02%), Query Frame = 0

Query:  308 EYLGPSF---KIIDGTDAPIHAFPWLVSLNYNG------EHFCGGSLINQNTVITAAHC-MDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVRIDGKFTLAGLASFSVTDCSAPFPAVFARISYFKRRRRPRSIDKQFPYRSRPYQPTSAFLAKESN------VLLKIVDGTQSRLHAFPWAASLS-----FVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFI-----RVTLGEHDLSRPD--ETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETNP-DVIRQVDLNIISLEQCTT-EWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQVLVGVTSFGTQNCSDP-VSSVYSRVTYFMDWISAALSVF 828
            +Y GP+    +I+ G  A     PW VSL  N       +HFCGG ++N+  ++T AHC + +  +    S   V  GEHDL   +  E     +V +I  H  Y  +  +  D+AL++L  PV+ N  + P+CL        ++     A+GWG + +    SD+L+ V +NI  N  C +   A+ +PI    +C      G E  C GDSGGPL   +DG++ L G+ SF        +P V+ R++ +    + R +    P     +   +   A + +       + KI+ G  +     PW ASLS     F    H CGG LI    I+TAAHC+++P     +      V LGE +L+ PD  E S++V    +RV+ HP +  +      DI ++ L+ PV  N  + PVCLP+      F       +GWG  +  +   D ++ V + +    +C     R L  +    A   T  G  GT   C+GDSGGPLM     K  L+G+TSFG+ NC  P    VY+ V   ++WI A ++ +
Sbjct:   25 KYKGPNVFTSRIVGGRSASRGEIPWQVSLMLNHPLIGPIDHFCGGVILNRYWILTTAHCFLSYSYIAVQPSYWTVRLGEHDLRKVDPNE--KWYKVASIHYHKNYILSQAN--DIALIKLKDPVQTNEDVGPVCLA--VPSKDYKNESCFASGWGKIDKTLDRSDVLKVVRVNIYSNEFCDKQYRALGIPIRSSHLCA-GNIKGKEGTCQGDSGGPLSCFVDGRWHLIGITSFGKGCAEVDYPGVYTRVASYASWMQ-RIMSAFMPSLVLLFLFAAGPAAAQHDCGVPHVFISKIIGGRSTAYGEVPWQASLSLKHPTFGRIPHFCGGVLINTRWILTAAHCIKSPNGPSLLDPAYWTVRLGEQNLNAPDSDEKSYSV----ERVVHHPGFNNY----NNDIGMMKLKVPVVLNNHVMPVCLPSAD--ADFVGEFCTTSGWGKVDPRSKVVDALQIVRIEVFDNSKCDIYTKRFLIPIGNRHACAGTLEGGKGT---CQGDSGGPLMCRRNDKYYLLGLTSFGS-NCVKPEFPDVYTNVKAHLNWIKAVVARY 555          

BLAST of transmembrane protease serine 9-like vs. nr
Match: gi|1330893070|gb|PNF23380.1| (hypothetical protein B7P43_G13178 [Cryptotermes secundus])

HSP 1 Score: 224.557 bits (571), Expect = 1.775e-59
Identity = 176/585 (30.09%), Postives = 286/585 (48.89%), Query Frame = 0

Query:  273 AEDTEELTKKRLFHELYNPSFS---------AKGVFCDSKFRNKEYLGPSFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQMLAVAAGWGVTEDQSSSDIL-RQVELNIIRNSTCVENMNAINVPILEDMICTFKGPLGSETICSGDSGGPLMVR-IDGKFTLAGLASFSVTDCSAPFPAVFARISYFKRRRRPRS----IDK---------QFPYRSRPYQPTSAFLAK-------ESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEF-IRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAF-LIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWGLTENETNPD-VIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVLEGTKQV-LVGVTSFGTQNCSDP-VSSVYSRVTYFMDWI 821
            AE+    + + LF EL+    S          +   C     N+E      +I+ G    ++ +PW+  L Y+G+  CGGSL+N + V+TAAHC+         SK+ V+ G+HD    +ET     R V  II H  +     +  D+ALL+L +PV + + ++P+CLP   +G           GWG T +      L  +V++ I+    C +     +  I  +M+C  K   G++  C GDSGGPL+V+ +D K  + GL    ++     +  ++A I   +    PR+    ++K         + P  SRP +    +  +       E N   +IV G  + ++ +PW   LS+   F+ CGG LI +  I+TAAHCV+  GF  F I+VT GEHD  R ++T     +   R +L  +   F  +    DIAL+ L D VP    I+P+CLP N+    ++    IA GWG  + +  P  ++++V++ ++S E C    +  + + ++  M+C    P G   +C+GDSGGPL      K+  L+G+ S+G   C+ P    VY+RVT ++ WI
Sbjct:   94 AENNTNGSNRLLFDELFGLGSSDTSDTDDEVPQNCSCQCGVANQE-----IRIVGGRPTGVNRYPWVARLVYDGKFHCGGSLLNGDYVLTAAHCVRRLK----RSKIRVFLGDHDQYQTSETP-AVMRAVSAIIRHRNFDVNTYN-HDIALLKLRKPVTFTKRVRPVCLP--HSGVDPAGREGTVVGWGRTSEGGMLPALAHEVQVPILSLQQCRQTKYRPS-RITANMLCAGK---GTQDSCQGDSGGPLVVQAVDDKLEIVGLLRGVISSSQGLY--LYANIHVLRSWVGPRAGRDDVEKRKFLTLPGLEGPPLSRPVRTQLLYRMRDPGSCCGERNDGSRIVGGKPTEMNEYPWMVRLSYFNRFY-CGGMLINDRYILTAAHCVK--GFMWFMIKVTFGEHD--RCNDTR----KPESRFVLRATTGDFSFMNFDHDIALLRLNDRVPITDTIRPICLPNNTD-EVYEGVKAIATGWGTVKEDGKPSCILQEVEVPVMSNEDCRKNTKYNEKM-ISDNMLCA-GYPEGMKDSCQGDSGGPLATERDDKKFELIGIVSWG-NGCARPGYPGVYTRVTRYLSWI 646          

BLAST of transmembrane protease serine 9-like vs. nr
Match: gi|1062976358|ref|XP_017904542.1| (PREDICTED: ovochymase-1 [Capra hircus])

HSP 1 Score: 228.409 bits (581), Expect = 1.786e-59
Identity = 168/548 (30.66%), Postives = 269/548 (49.09%), Query Frame = 0

Query:  315 KIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLPKLEAGGSFEQ-MLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINVPIL-EDMICTFKGPLGSETICSGDSGGPLMVRI-DGKFTLAGLASFSV--TDCSAPF--------PAVFARISYFKR-------------------RRRPRSIDKQFPYRSRPYQPTS-AFLAKESNVLLKIVDGTQSRLHAFPWAASLSFVGGFHGCGGSLIRNDMIITAAHCVETPGFAEFIRVTLGEHDLSRPDETSFTVTRRVKRVILHPSYTAFLIGLPGDIALIILEDPVPFNQGIKPVCLPTNSPVGTFDNRMGIAAGWG-LTENETNPDVIRQVDLNIISLEQCTTEWRLLDGLELTPAMICTFNGPLGTGSTCRGDSGGPLMVL-EGTKQVLVGVTSFGTQNCSDPVS-SVYSRVTYFMDWISAAL 825
            +I    ++ +   PW VSL   G HFCGGSLI  + V+TA HC+           L V  GE++L   ++ E  +   V  IIIHP Y+       ++ALL L   V++   ++PIC+P    G  FE+ +L +A+GWG ++E    S+IL++  + I+ + TC   +  +++P L  DM+C    P G +  C  DSGGPL+ R  DG + LAG+ S++   T    PF        P +F+++                        +  P S  K  PY      P S  +L++      +I  G ++  H +PW   L F+G  H CGG++I +  I+TAAHCV++     F  +  G+HD  R  + S    RR K +++H  +    +    DIALI L   + FN  ++PVCLP +S    F + + +  GWG ++++      ++Q+ + ++  E C   +       ++  MIC      G     +GDSGGPL+   E    VL G+ S+G   C+ P    V++RV+ F+DWI + +
Sbjct:   36 RISSWRNSTVGGHPWQVSLKLGGHHFCGGSLIQDDLVVTAVHCL-ISLNEKQIKSLTVTAGEYNLFQKDKEEQNSP--VSKIIIHPEYNRLGYMSFNIALLYLKLKVKFGTTVQPICIP--HRGDKFEEGILCMASGWGKISETSEYSNILQEAVVPIMDDRTCGAMLRGMSLPPLGRDMLCA-SFPHGEKDACQRDSGGPLVCRRDDGAWVLAGITSWAAGCTRVWNPFRNKQRKATPGIFSKVFVLMDFITQTMTDSLNKIGSTSFPKTNPISSAKVIPYGVCGIPPFSPQWLSR------RIAGGVEACPHCWPWQVGLRFLGNHH-CGGAIISSIWILTAAHCVQSKNNPLFWTIVAGDHD--RTLKESTEQVRRAKHIVMHEDFDT--LSYDSDIALIQLSSALEFNSVVRPVCLP-HSLEPLFSSEICVVTGWGSVSKDGGLASRLQQIQVPVLEREVCERTYYSAHPGGISEKMICAGFAASGGKDVGQGDSGGPLVCKHEKGPFVLYGIVSWGA-GCAQPRKPGVFARVSVFLDWIQSKI 564          

HSP 2 Score: 130.568 bits (327), Expect = 2.192e-27
Identity = 83/241 (34.44%), Postives = 130/241 (53.94%), Query Frame = 0

Query:  313 SFKIIDGTDAPIHAFPWLVSLNYNGEHFCGGSLINQNTVITAAHCMDFGAVPDFFSKLLVYFGEHDLSLANETEFTTTRRVLNIIIHPRYSATPGSPGDVALLQLDRPVEYNRAIKPICLP-KLEAGGSFEQMLAVAAGWG-VTEDQSSSDILRQVELNIIRNSTCVENMNAINV-PILEDMICTFKGPLGSETICSGDSGGPLMVRID-GKFTLAGLASFSVTDCSAPF-PAVFARISYF 548
            S +I  G +A  H +PW V L + G H CGG++I+   ++TAAHC+     P F++   +  G+HD +L   TE    RR  +I++H  +  T     D+AL+QL   +E+N  ++P+CLP  LE    F   + V  GWG V++D   +  L+Q+++ ++    C     + +   I E MIC      G + +  GDSGGPL+ + + G F L G+ S+    C+ P  P VFAR+S F
Sbjct:  325 SRRIAGGVEACPHCWPWQVGLRFLGNHHCGGAIISSIWILTAAHCVQSKNNPLFWT---IVAGDHDRTLKESTE--QVRRAKHIVMHEDFD-TLSYDSDIALIQLSSALEFNSVVRPVCLPHSLEP--LFSSEICVVTGWGSVSKDGGLASRLQQIQVPVLEREVCERTYYSAHPGGISEKMICAGFAASGGKDVGQGDSGGPLVCKHEKGPFVLYGIVSWG-AGCAQPRKPGVFARVSVF 556