hypothetical protein DAPPUDRAFT_304927, maker-scaffold483_size159862-snap-gene-0.41 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Match: EMLSAG00000009829 (supercontig:LSalAtl2s:LSalAtl2s63:1003125:1030351:1 gene:EMLSAG00000009829 transcript:EMLSAT00000009829 description:"maker-LSalAtl2s63-augustus-gene-10.41") HSP 1 Score: 114.39 bits (285), Expect = 1.275e-25 Identity = 151/558 (27.06%), Postives = 239/558 (42.83%), Query Frame = 0
Query: 181 LRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFAC--GCDLAWLTDWLQKNPVETSGARCETPVKLQRKK---ISSLHL----GR-SNCLDLSSASCNWTSPCPFPCSCSEGV-----VDCS-------RRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGL----DGLTTLHLHNNNIHCISPGAF---DLLPKLSFTNILSNPIQCDCHMAWF-GDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASEL-YLDV--NDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVF--ANLTRLSTLIISYNEIKCIQPHAF-SGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCD 702
L L +NNI I P F + L+ +DLS N +R + DD F GL L L L N+I ++ ++ LP+L+ + N I I F ++ L L+L N I + TF LSSL+ L+L N G L D L+ + +G P+ K + S +L GR N DL+ + EG+ +D S GV I P E+ LS N L + + G F LP L L++S N IS+I++G+F+ + +DLSHN L++ + + L TL + +N + + P +F LL ++ F+N L ++F +++ N L +LR L+ + F + V+ H ++++V + +S+L +LD+ N I +LE F RL LD+S+N +S + +F + +L +L +S+N+ I S L IL + N IS IP+ A N I + L NPL D
Sbjct: 320 LDLSRNNIHEIIPGTFLGIGNLKGLDLSVNSLRKVEDDAFEGLTSLEYLDLSDNKISQLPSNALERLPNLKRFKAHHNTIDSISSEDFIPIQDLEELNLSYNMISQIFPNTFASLSSLKILNLEYNFIKSLEGTAFQGLEDLLEYLNLAHNGIEILGPLNFSALKYINLGSNYLEDIEGRFKNLRDLNVLILRYNKITNLNAKTLEGIDNLIKLDISGNELSDLEPGVLINP-----LLNEINLSSNALSEI-NTGTFKELPILEMLDISSNKISMIKKGAFDSIPRLKTIDLSHNQLSSYKDDYFFNMYPNDTDLHTLDISHNELTYLYPESFRFHQLLERVDFSNNL---------FSFFPTQFMKDLPNLKTL-------NLRHNLLKNIDDSEF------------------ANFLSLKVLNLEHNEVESVSETAFQNSSQLQFLDLSHNRISNLEGDTFKGTIRL-LLDLSHNNLSSMPNGIFDRPKVVKLQSLDLSHNKFLRIPVDVLQSQYFFLDILKVSNNLISEIPSDANILVN-IKEIDLSFNPLSED 835 HSP 2 Score: 113.235 bits (282), Expect = 2.728e-25 Identity = 123/532 (23.12%), Postives = 217/532 (40.79%), Query Frame = 0
Query: 177 GATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKE----LQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSN-----VPKEASELYLDV--NDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSN 697
G EL L N + ++P +A LK LR ++L +N I+ I + F L L L L N I I +F + +L+ L L+ N +R + +AF L L L L DN I L + L +L+ N +++ + P++ + L+LS + P F S +++ +K + + ++ L L+ N +++ +G N AL +NL N++ I EG F+ R +++L L +N +T + + L G+D L L + N + + PG +L NP+ + +++ ++AL+ T +ELP+ S +++ + + I SH QL + + P + LD+ N++ L F Q L R+D SNN S +L L TL + +N +K I F+ L++L+L N + + +AF + + + L L N
Sbjct: 268 GLVELDLSSNFLSTVPMQALVPLKTLRFLNLGSNLIKSIKEANFEALSSLEYLDLSRNNIHEIIPGTFLGIGNLKGLDLSVNSLRKVEDDAFEGLTSLEYLDLSDNKISQLPSNALERLPNLKRFK------------------AHHNTIDSISSEDFIPIQDLEE------------LNLSYNMISQIFPNTFASLSSLKILNLEYNFIKSLEGTAFQGLEDLLEYLNLAHNGIEI---LGPLN-FSALKYINLGSNYLEDI-EGRFKNLRDLNVLILRYNKITNLNAKTLEGIDNLIKLDISGNELSDLEPG------------VLINPLLNEINLS-----------SNALSEINTGT------FKELPILEMLDISSNKISMIKKGAF--DSIPRLKTIDLSHNQLSSYKDDYFFNMYPNDTDLHTLDISHNELTYLYPESFRFHQLLERVDFSNNLFSFFPTQFMKDLPNLKTLNLRHNLLKNIDDSEFANFLSLKVLNLEHNEVESVSETAFQNSSQLQFLDLSHN 733 HSP 3 Score: 99.3673 bits (246), Expect = 4.398e-21 Identity = 132/560 (23.57%), Postives = 216/560 (38.57%), Query Frame = 0
Query: 180 ELRLEQNNIVSIPPKAFKKLKWLRRIDLSNN--------------------------EIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNC--LDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPA--LSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGL-DGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELP-LPSFRCKGSE----DEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANL----TRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPL 699
+L++ N + +P A + K L+ + L +N IR+I TF + RL L+L N++ I F +L L L L+SN + + A L+ L L+L N I+++ F LSSL+ L L +N + I LG N LDLS S F S +D S + +PS+ + L+ + + S+ + +P L LNLS N IS I +F S+ IL+L +N + ++ G +GL D L L+L +N I + P F L + N+ SN ++ D G + ++ + +L + L+ + L G+E + G L + + + + K +P L + N I ++ F + RL +D+S+N++S K F N+ T L TL IS+NE+ + P +F L + N S P D ++ L L N L
Sbjct: 173 DLKINGNKLKKVPTGALRGPKSLQNLFLQDNQISNGVKLGGSFITQPKLLSLNLTNNLIRVIRPTTFANVTRLVRLILTRNKLSSIVSDEFKELTGLVELDLSSNFLSTVPMQALVPLKTLRFLNLGSNLIKSIKEANFEALSSLEYLDLSRNNI--------------------------------HEIIPGTFLGIGNLKGLDLSVNSLRKVEDDAFEGLTSLEYLDLSDNKISQLPSNALERLPNLKRFKAHHNTIDSISSEDFIPIQDLEELNLSYNMISQIFPNTFASLSSLKILNLEYNFIKSLEGTAFQGLEDLLEYLNLAHNGIEILGPLNFS---ALKYINLGSNYLE-DIE----GRFKNLRDLNVLILRYNKITNLNAKTLEGIDNLIKLDISGNELSDLEPGVLINPLLNEINLSSNALSEINTGTFKELPI-----LEMLDISSNKISMIKKGAFDSIPRLKTIDLSHNQLSSYKDDYFFNMYPNDTDLHTLDISHNELTYLYPESFRFHQLLERVDFSNNLFSFFPTQFMKDLPNLKTLNLRHNLL 687 HSP 4 Score: 95.5153 bits (236), Expect = 6.836e-20 Identity = 134/520 (25.77%), Postives = 202/520 (38.85%), Query Frame = 0
Query: 196 FKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPK---TTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPK--------LSFTNILSNPIQCDCHMAWFGDWLRSQNN--TSALATCATPAHLRDRQLQELPLPSFRCKGSEDE--GCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIK---PYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSI-SMIPNSAFDDHNSITHLALGS 696
FK L+ L + L N+I + T G+ L L + GN++ + + P L + L+SN + I+ F L +L +L + N I + G F+ + L+T+ L N + D + + P +T LH LD+S + P F VD S P+ K K L L N+L + N L +L LNL N + + E +F+ + + LDLSHN ++ + G +G L L L +NN+ + G FD PK LS L P+ +F D L+ NN + + ++++ L PL SED L + R GT VPS S L L N I +L + + L +DIS+N+I + + LT L L IS N +K I F L LR L + S+ + I AF D + L L S
Sbjct: 500 FKNLRDLNVLILRYNKITNLNAKTLEGIDNLIKLDISGNELSDLEPGVLIN-PLLNEINLSSNALSEINTGTFKELPILEMLDISSNKISMIKKGAFDSIPRLKTIDLSHNQLSSYKDDYFFNMY----PNDTD-----------------LH-----TLDISHNELTYLYPESFRFHQLLERVDFSNNLFSFFPTQFMKDLPNLKTLNLRHNLLKNIDDSEFANFL-SLKVLNLEHNEVESVSETAFQNSSQLQFLDLSHNRISNLEGDTFKGTIRL-LLDLSHNNLSSMPNGIFD-RPKVVKLQSLDLSHNKFLRIPVDVLQSQYFFLDILKVSNNLISEIPSDANILVNIKEIDLSFNPL-------SEDSVYNVLNEPKTVRSLNMAGT-------GXTVVPSLETPFLSSLNLSHNFISELNEDNLNK-PSLEVIDISHNEIPDLSYGLSSAWPLLTNLKYLDISSNPVKFIIKGDFKYLDGLRSLKMSNLSLCNRIEGDAFKDLKELKELDLYS 974 HSP 5 Score: 94.3597 bits (233), Expect = 1.590e-19 Identity = 134/532 (25.19%), Postives = 208/532 (39.10%), Query Frame = 0
Query: 180 ELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERIS--GSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNV----------PKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPL 699
EL L N++V I F+ K L+ + ++ N+++ +P G L +L L NQI G SF P L L L +N IR I P F + L L L N + ++ + F L+ L L L N + T + P+K R L+LG + + A+ F S +D SR +N+ +++P G F + L L+LS N + +E+ +FEG S+ LDLS N ++ + L L L H+N I IS F + L N+ N I + NT A + +L ++ L +F +G ED LE + + S + + SN ++ + L L N I +L + L +LDIS N++S ++P V N L+ + +S N + I F L L +L + N ISMI AFD + + L N L
Sbjct: 149 ELDLSSNSLVDIDDGLFRGCKNLKDLKINGNKLKKVPTGALRGPKSLQNLFLQDNQISNGVKLGGSFITQPKLLSLNLTNNLIRVIRPTTFANVTRLVRLILTRNKLSSIVSDEFKELTGLVELDLSSNFLS------------------TVPMQALVPLKTLR----FLNLGSNLIKSIKEAN--------FEALSSLEYLDLSR--------------------NNIHEIIP--GTFLGIGNLKGLDLSVNSLRKVEDDAFEGLTSLEYLDLSDNKISQLPSNALERLPNLKRFKAHHNTIDSISSEDFIPIQDLEELNLSYNMIS------------QIFPNTFASLSSLKILNLEYNFIKSLEGTAF--QGLED--LLEYLNLAHNGIEILGPLNFSALKYINLGSNYLEDIEGRFKNLRDLNVLILRYNKITNLNAKTLEGIDNLIKLDISGNELSDLEPGVLIN-PLLNEINLSSNALSEINTGTFKELPILEMLDISSNKISMIKKGAFDSIPRLKTIDLSHNQL 611 HSP 6 Score: 91.2781 bits (225), Expect = 1.439e-18 Identity = 128/540 (23.70%), Postives = 203/540 (37.59%), Query Frame = 0
Query: 202 LRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQI------ERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDL--AWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTK---ELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELP-LPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELY---LDVNDIDDLEMAHFSHLQR-LSRLDISNNKISVIKP-------YV-------------FANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNL 705
L+ +D S+N+IR + + F G+ ++ GN + S S F +L +L+ L L+SN + I F + L L + N ++ + G G SLQ L L N + G L +++T P S +++ R P F + +R + I SD K EL LS N L VP L L L LNL N I I+E +FE S+ LDLS N++ I G+ L L L N++ + AF+ L L + ++ N I + L L+ LP L F+ + + + + P + + + S+ + + N S L L+ N I LE F L+ L L++++N I ++ P Y+ F NL L+ LI+ YN+I + G+ +L L + GN +S + NPLL + NL
Sbjct: 93 LKHLDFSHNQIRRLTERLFDGIEDSLEVLRLGNNLLGYNLNPVYSSSEFQNLGALRELDLSSNSLVDIDDGLFRGCKNLKDLKINGNKLKKVPTGALRGPKSLQNLFLQDNQISNGVKLGGSFIT-----QPKLLSLNLTNNLIRVIR-------------------------PTTFANVTRLVRLILTRNKLSSIVSDEFKELTGLVELDLSSNFLSTVPMQALVPL-KTLRFLNLGSNLIKSIKEANFEALSSLEYLDLSRNNIHEIIPGTFLGIGNLKGLDLSVNSLRKVEDDAFEGLTSLEYLDLSDNKI----------------------------SQLPSNALERLPNLKRFKAHHNTIDSISSEDFIPIQDL---EELNLSYNMISQIFPNTFASLSSLKILNLEYNFIKSLEGTAFQGLEDLLEYLNLAHNGIEILGPLNFSALKYINLGSNYLEDIEGRFKNLRDLNVLILRYNKITNLNAKTLEGIDNLIKLDISGNELSDLEPGVL------------INPLLNEINL 558
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Match: EMLSAG00000012107 (supercontig:LSalAtl2s:LSalAtl2s869:139642:146365:-1 gene:EMLSAG00000012107 transcript:EMLSAT00000012107 description:"maker-LSalAtl2s869-augustus-gene-1.6") HSP 1 Score: 110.538 bits (275), Expect = 1.284e-24 Identity = 157/665 (23.61%), Postives = 251/665 (37.74%), Query Frame = 0
Query: 172 ASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEI--RLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLG--------KNAFACGC----DLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCL------DLSSASCNWTSPC---PFPC------------SCSEGVVDCSRRGVKIIPSD----------------------------------IPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILS----------NPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQ----------ELPLPSFRCKGS----------EDEGCLEQHYCPRECVC------TGTVIRCSHAQLKTVPSNVPKEASELYLDVND-------------------------IDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGN---SISMIPNSAFDDHNSITHLALGSNPLLCDC 703
A PH EL L+ N++ + PK F L L +++S NEI + F L RL SL L N+I + S L SLQ+L L+ N + + F LH+L L N++++L +F+GLS + +++L +NAF C DL + + P G + L I +H G L LSS S P P S +GV + R +K++ D IPK + L + N ++ + + L T + S N I +E SF +SI + +L+HN + + + R L + L +N + + +F++ P T+I NP+ CDC M WF N + + P + +Q ++P F C+ + C + CP C C + VI+CS VP +P +A+ +Y+D N+ I ++ F+ L L L + +N++ ++ Y F N++ L L + N I+ I F L LRIL+L GN S S+ +T + L +NP C C
Sbjct: 277 APTPH-LRELSLQNNSLGVLAPKIFSGLSRLLVLNVSRNEIGNEWLTPALFASLVRLVSLDLSHNRISSLDPSVLRPLTSLQILDLSHNLLTTLDSGTFLSQNNLHILRLNHNDLKSLHARSFDGLSVVSSINLDSNALSALEENAFLDCCSTLNDLTLSNNRFSRVPDGLQGLDGLKTLDLGGNNIKEIHNGSFTGLRALYGLKLSSNGITNISESIFKPIPGVQVLNLAFNKIKSLGQGVFN-KLRNLKMLRLDGNELIDINGLLSGQEELQWLNVSGNRLQWFDYAFIPKNLELLDIHGNQIENLGNYXKLTDQFELKTXDXSHNKIKKLETLSF--PKSIQVANLNHNRIRLLTPGMFRDKIKLRKVDLSHNYLKRLQISSFNIAPYSESTSIQEEPRPHFYMGHNPLHCDCEMEWF-------KNMNEMTQARLPIIMDYSSIQCHGDSSTSILDIPNEHFLCQYEAHCFSLCMCCDFFACDCRMQCPEGCDCYHDSSWSVNVIQCSXRHHALVPPLIPMDATNIYMDGNNFTGLLDTQSFIGRKRISSMFLNNSNIGGIDNQTFNGLMELKILHLEDNQVQRLEGYEFVNISSLQELYLHRNRIEYIHERTFDNLLSLRILTLDGNNLISTSIWEFPLLSSLQDLTSITLSNNPWSCKC 930 HSP 2 Score: 97.0561 bits (240), Expect = 1.943e-20 Identity = 153/601 (25.46%), Postives = 244/601 (40.60%), Query Frame = 0
Query: 181 LRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTS-------LVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCD--LAWLTDWLQKNPVETS-GARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKEL---QLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSF-EGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSE---DEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDL-EMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFD-----DHNSIT-----HLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANH-----LVLTTPESSFKCQAE 748
L L NN+ S+PP F +L L ++LS N ++ + + +F+ R TS L+L N I + + L+ L L+SN I + NAF+ L L LSL +N + L TF L+ L L N+ + L+ L N G TP L R LDLS + P S ++D S + + S + L +L+ N L + + F+ L +S++NL N +S +EE +F + +++ L LS+N + + L +GLDGL TL L NNI I G+F L L + SN I + + F Q A + +L+ L + R G+E G L + +G ++ +PK L + N I++L + L D S+NKI ++ F +++ L ++N I+ + P F LR + L N + + S+F+ + SI H +G NPL CDC ++W N+ + + S+ H +L P F CQ E
Sbjct: 158 LNLSHNNLWSVPPGTFCRLSGLSTLNLSFNRLQDMHELSFSPSKRSTSCKLPLENLILSDNSYVEIPSQALSHVSKLETLELSSNAINLVQDNAFDNLRDLKYLSLANNQLIALPPHTFAPTPHLRELSLQNNSLGVLAPKIFSGLSRLLVLNVSRNEIGNEWLTPALFAS-------LVRLVSLDLSHNRISSLDPSVLRPLTSLQILDLSHNLLTTLDSGTFLSQNNLHILRLNHNDLKSLHARS-FDGLSVVSSINLDSNALSALEENAFLDCCSTLNDLTLSNNRFSRVPDGL-QGLDGLKTLDLGGNNIKEIHNGSFTGLRALYGLKLSSNGI-TNISESIFKPIPGVQVLNLAFNKIKSLGQGVFNKLRNLKM--LRLDGNELIDINGLLSGQEELQWLNVSGNRLQWFDYAF------IPKNLELLDIHGNQIENLGNYXKLTDQFELKTXDXSHNKIKKLETLSFPKSIQVANL--NHNRIRLLTPGMFRDKIKLRKVDLSHNYLKRLQISSFNIAPYSESTSIQEEPRPHFYMGHNPLHCDCEMEWFKN--MNEMTQARLPIIMDYSSIQCHGDSSTSILDIPNEHFLCQYE 736 HSP 3 Score: 84.3445 bits (207), Expect = 1.446e-16 Identity = 58/182 (31.87%), Postives = 92/182 (50.55%), Query Frame = 0
Query: 144 DSRVNCPKECHC------NDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSI-PPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQ---TLHLGKNAFACGCDLA 315
D R+ CP+ C C + ++ C + P IP AT + ++ NN + ++F K + + L+N+ I I + TF GL L L L NQ++R+ G F ++ SLQ L L+ N+I IH F+ L L +L+L NN+ + F LSSLQ ++ L N ++C C+
Sbjct: 752 DCRMQCPEGCDCYHDSSWSVNVIQCSXRHHALVPPLIPMDATNIYMDGNNFTGLLDTQSFIGRKRISSMFLNNSNIGGIDNQTFNGLMELKILHLEDNQVQRLEGYEFVNISSLQELYLHRNRIEYIHERTFDNLLSLRILTLDGNNLISTSIWEFPLLSSLQDLTSITLSNNPWSCKCEFV 933 HSP 4 Score: 81.2629 bits (199), Expect = 1.136e-15 Identity = 119/489 (24.34%), Postives = 199/489 (40.70%), Query Frame = 0
Query: 196 FKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSL-VLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTT---KELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFI--SVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAH-LRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDV--NDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSI 675
F L L+ + + ++ IP +F GL LTSL V+ + +E S +F L L+ L L+ N + + P F RL L L+L +FN L + L + + C L P+E + V++ + +S H+ + L+LSS + N F + + + +P T +EL L +N L V+ + +F+ L L LN+SRN I + F + LDLSHN ++++ +LR L L L L +N + + G F SQNN L L R L + S S LE++ +C T + S+ + VP + LD+ N+I ++ F+ L+ L L +S+N I+ I +F + + L +++N+IK + F+ L +L++L L GN +
Sbjct: 100 FGHLPTLKELRIQGCPMKKIPSGSFQGLGGLTSLSVVTQDSLELDSERAFEGLDHLRSLNLSHNNLWSVPPGTFCRLSGLSTLNL-----------SFNRLQDMHELSFSPSKRSTSCKL----------PLENLILSDNSYVEIPSQALS--HVSKLETLELSSNAINLVQDNAFDNLRDLKYLSLANNQLIALPPHTFAPTPHLRELSLQNNSLGVL-APKIFSGLSRLLVLNVSRNEIGNEWLTPALFASLVRLVSLDLSHNRISSLDPSVLRPLTSLQILDLSHNLLTTLDSGTF-----------------------------LSQNNLHILRLNHNDLKSLHARSFDGLSVVSSINLDSNALSALEEN-AFLDCCSTLNDLTLSNNRFSRVPDGLQGLDGLKTLDLGGNNIKEIHNGSFTGLRALYGLKLSSNGITNISESIFKPIPGVQVLNLAFNKIKSLGQGVFNKLRNLKMLRLDGNEL 534 HSP 5 Score: 77.0258 bits (188), Expect = 2.532e-14 Identity = 80/242 (33.06%), Postives = 110/242 (45.45%), Query Frame = 0
Query: 71 SIDLSFNSITQF-PDVSEWLEYAQIRHIDVSHNFIECFDS-NLVNHKVEHVLELKH----GRGCRSDEDLLTAGESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKN 306
S+DLS N I+ P V L QI +D+SHN + DS ++ H+L L H RS D L+ SI LDS E + +DC +L L NN S P + L L+ +DL N I+ I + +FTGL L L L N I IS S F +P +Q+L L NKI+ + FN+L L +L L N + + NG +G LQ L++ N
Sbjct: 334 SLDLSHNRISSLDPSVLRPLTSLQI--LDLSHNLLTTLDSGTFLSQNNLHILRLNHNDLKSLHARS-FDGLSVVSSINLDSNALSALE---ENAFLDCCST------------LNDLTLS-NNRFSRVPDGLQGLDGLKTLDLGGNNIKEIHNGSFTGLRALYGLKLSSNGITNISESIFKPIPGVQVLNLAFNKIKSLGQGVFNKLRNLKMLRLDGNELIDI-NGLLSGQEELQWLNVSGN 555 HSP 6 Score: 72.7886 bits (177), Expect = 5.635e-13 Identity = 84/301 (27.91%), Postives = 128/301 (42.52%), Query Frame = 0
Query: 414 GLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILD-LSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAH---LRDRQLQELPLPSFRCKGSEDEGCLEQHYC------PRECVCTGTVIRCSHAQLKTVPSNV---PKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKI--SVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPL 699
G F LP L L + + I GSF+G + L ++ +SL R GLD L +L+L +NN+ + PG F L LS N+ N +Q D H F RS +C P L D E+P + + L + + + + ++ QL +P + EL L N + L FS L RL L++S N+I + P +FA+L RL +L +S+N I + P L L+IL L N ++ + + F N++ L L N L
Sbjct: 98 GHFGHLPTLKELRIQGCPMKKIPSGSFQGLGGLTSLSVVTQDSLELDSERAFEGLDHLRSLNLSHNNLWSVPPGTFCRLSGLSTLNLSFNRLQ-DMHELSFSPSKRS-------TSCKLPLENLILSDNSYVEIPSQALSHVSKLETLELSSNAINLVQDNAFDNLRDLKYLSLANNQLIALPPHTFAPTPHLRELSLQNNSLGVLAPKIFSGLSRLLVLNVSRNEIGNEWLTPALFASLVRLVSLDLSHNRISSLDPSVLRPLTSLQILDLSHNLLTTLDSGTFLSQNNLHILRLNHNDL 390
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Match: EMLSAG00000012217 (supercontig:LSalAtl2s:LSalAtl2s887:351250:355137:-1 gene:EMLSAG00000012217 transcript:EMLSAT00000012217 description:"maker-LSalAtl2s887-augustus-gene-3.4") HSP 1 Score: 107.071 bits (266), Expect = 1.869e-23 Identity = 148/633 (23.38%), Postives = 256/633 (40.44%), Query Frame = 0
Query: 179 TELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIR--LIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFN----GLS------------------SLQTLHLGKNAF---------ACGCDLAWLTDWLQKNPVETSGARCET------PVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCS-------------------EGVV---------DCSRRGVKIIPSD-IPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIH-------CISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELP-------------LPSFRCKGSEDEGCLEQHYCPRECVC------------------------TGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPL 699
T+L L N+++ +P + F+ L+ L ++LS N I + + F GL +L +L L N++ R+ + F L SL++LL++SN++ I ++FN + L + L N QT + FN LS +L++L+LGKN+F +CG L L L +N +E+ T ++L + IS L+L + L+L + S C GVV + S ++ IP+ + L L N + + + AL +++S N ++ + +F HI +++N +T + L ++ + L NN + IS G+ ++ P+++ NP CDC M DW+R N + L H + R L +L L ED C + +C C+C VI CS + +P +P +++E+ LD N I ++ F R+ L ++N+ + ++ + L++L + N+I + + F L LR L LH N++ I F S+T L LG N L
Sbjct: 267 TDLSLHNNSLIGLPSQIFESLESLVVLNLSRNVISDSYLSQEAFAGLSKLLTLDLSHNRLRRLQPALFESLVSLEVLLMDSNELY-IMESSFNLFKNLRIFGLSSNYFQTFVSHFFNPELKSLSLEGNVLNELPDDLFSNGCALESLNLGKNSFGSVPKFIASSCGSSLKTLD--LGENSIESIREHDLTNLTELKGLRLAKNYISELNLKNFDLLNLEVLNLASNSLKKLEPGCLGNMTSLKALRLDKNKLTDLNGVVSGYPNLLWLNVSHNKIQWFDYGFIPRQLERLDLRHNDISELGNYYETKDKFALQFIDVSYNKLTSLNAETFPFKHFKHI-AINNNMITEVAQSTFAHLTKISKVELRNNFLRNMRMVSLSISSGSQEI-PQMTLN---GNPFVCDCEM----DWMRDINEIALLGQ----QHFQIRDLNDLTCELTHKNNGEIEQLKKIIAVPREDFLCSYRTHCFSLCMCCDFFACDCRMQCPEGCDCYHDQTWNRNVISCSASNHTEIPLLIPMDSTEVRLDGNVISNVSSQSFHGRHRVKSLFLNNSDVFILGNQTLTGXSSLNSLHLEDNQISHLYGNEFLNLKKLRELFLHNNALEFINELTFAPLESLTTLTLGGNLL 883 HSP 2 Score: 72.7886 bits (177), Expect = 5.034e-13 Identity = 51/178 (28.65%), Postives = 86/178 (48.31%), Query Frame = 0
Query: 144 DSRVNCPKECHC------NDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLS-SLQTLHLGKNAFACGCDL 314
D R+ CP+ C C N ++ C P IP +TE+RL+ N I ++ ++F ++ + L+N+++ ++ + T TG L SL L NQI + G+ F +L L+ L L++N + I+ F LE L L+L N + + + S L L N ++C C
Sbjct: 737 DCRMQCPEGCDCYHDQTWNRNVISCSASNHTEIPLLIPMDSTEVRLDGNVISNVSSQSFHGRHRVKSLFLNNSDVFILGNQTLTGXSSLNSLHLEDNQISHLYGNEFLNLKKLRELFLHNNALEFINELTFAPLESLTTLTLGGNLLTSFPVWRLLEFNPSTSVLDLSGNPWSCECQF 914 HSP 3 Score: 56.225 bits (134), Expect = 6.562e-8 Identity = 82/355 (23.10%), Postives = 140/355 (39.44%), Query Frame = 0
Query: 174 IPHGATELRLEQNNIVSIPPKAFKKLKW-LRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGAR-----------CETPVK-----LQRKKISSLHLG------RSNCLDL----SSASCNWTSPCPFPCSC------SEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLS 495
IP L L N+I + K K+ L+ ID+S N++ + +TF + + N I ++ S+F L + + L +N +R N++ + +G + + L N F C C++ W+ D N + G + CE K Q KKI ++ R++C L +C+ CP C C + V+ CS IP IP + E++L NV+ V S F+ + +L L+ + + ++ + G S++ L L N ++ + G L L L LHNN + I+ F L L+
Sbjct: 546 IPRQLERLDLRHNDISELGNYYETKDKFALQFIDVSYNKLTSLNAETFP-FKHFKHIAINNNMITEVAQSTFAHLTKISKVELRNNFLR---------------------NMRMVSLSISSGSQEIPQMTLNGNPFVCDCEMDWMRDI---NEIALLGQQHFQIRDLNDLTCELTHKNNGEIEQLKKIIAVPREDFLCSYRTHCFSLCMCCDFFACDCRMQCPEGCDCYHDQTWNRNVISCSASNHTEIPLLIPMDSTEVRLDGNVISNVSSQS-FHGRHRVKSLFLNNSDVFILGNQTLTGXSSLNSLHLEDNQISHLYGNEFLNLKKLRELFLHNNALEFINELTFAPLESLT 874 HSP 4 Score: 55.8398 bits (133), Expect = 8.602e-8 Identity = 118/479 (24.63%), Postives = 187/479 (39.04%), Query Frame = 0
Query: 244 FYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQ------TLDNGTFNGLSSLQTLHLGKNAF-------------ACGCDLAWLTDWLQKNPVETS---GARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEG--VVDCSRRGVKIIP------SDIPKTTK-------------ELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFI--SVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQL-KTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSIS 676
F +LPSL+ L + KIR + P +F L L LS+ +N Q L++ +F L + L+L N DL++ L + + G CE V + I+ L+L +L S+ N P EG V D + + + +DI + K +L L +N L +PS +F L +L LNLSRN I S + + +F G + LDLSHN L ++ L L L L + +N ++ I +F+L L + SN Q +F L+S L L ELP F + C E + G + S + K + S+ L L N I+ + ++L L L ++ N IS + F +L L L ++ N +K ++P + L+ L L N ++
Sbjct: 77 FGNLPSLEELDIQFCKIRHLPPRSFAGLSNLKKLSIQSHNTQWASIIMELESSSFYNLGQVTDLNLAHNNLRSIPPRTLCSTPQLTSLDLSYNNILLLEQEDHSRLNLGGYCEDGVSV---NITDLNLSY----NLISSLSNVVLPRSIRNLNLEGNRVSDIQIKSLTNLENLNLGGNDINRLNKDSFALHGQNSLLTDLSLHNNSLIGLPS-QIFESLESLVVLNLSRNVISDSYLSQEAFAGLSKLLTLDLSHNRLRRLQPALFESLVSLEVLLMDSNELY-IMESSFNLFKNLRIFGLSSNYFQTFVS-HFFNPELKS-------------LSLEGNVLNELPDDLF------------SNGCALESLNLG---KNSFGSVPKFIASSCGSSLKTLDLGENSIESIREHDLTNLTELKGLRLAKNYISELNLKNF-DLLNLEVLNLASNSLKKLEPGCLGNMTSLKALRLDKNKLT 516
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Match: EMLSAG00000007692 (supercontig:LSalAtl2s:LSalAtl2s447:512720:516392:1 gene:EMLSAG00000007692 transcript:EMLSAT00000007692 description:"maker-LSalAtl2s447-snap-gene-4.51") HSP 1 Score: 105.531 bits (262), Expect = 5.110e-23 Identity = 145/555 (26.13%), Postives = 229/555 (41.26%), Query Frame = 0
Query: 180 ELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNA-----FACGCDLAWL-TDWLQKNPVETSGARCETPVKLQRKKISSLHLG------RSNC-----LDLSSASCNWTSPCPF--PCSCSEGVVDCSR-RGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQ-----------CDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTG-TVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSA---FDDHNSITHLALGSNPL 699
+L L +N + S+P AFK L +D+SNN+ + F L +L L + N+I RI F L++L LN+NKI + N+ + L L L DN I ++ G F +S + T+ L +N + DL ++ T LQ+N ++ + + L + IS L NC LDLS S F +E ++ ++ + +P K K L +S N + +P F L L L+ S N IS+I + F ++ +DLSHNSLT + + + L L L N ++ + F L + F + N +Q DC S NN + P + + L EL L + S G + + G + + L+++ S + L LD N I +L F +L L L +SNN I + F L +L L +S N I I P AF G+ L +++ N I + N F+D S+ + L N +
Sbjct: 196 KLSLFKNQLSSLPRDAFKSQGQLEFLDISNNQFSKLEAHYFPKLRKLLWLNISHNEIPRIQSRVFARNSLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDNFIPSVGRGAFRAVSRIGTVDLARNNLTKVDYQLFSDLRFIDTISLQENQIKNIDKEAFSNLYLTKINISHNQLPFLSSGLFKNCENMTFLDLSHNQIAHISEDAFNENTYATEFLLQYNKLTSMSGVPLASQKGLKFLNMSHNAIVDIPR-NTFPKLYELFRLDFSYNNISIIGKSIFSSLLTLRHIDLSHNSLTKLDSGMFGKVPTLLDLDLSYNKLNQVRRSVFGGLSSIRFVKLHHNQLQEIPSPPISLNTMDC----------SYNNIKEIK-GRQPWPVMN-SLLELNLDYNQLGNSIKAGRFDGLIVLKSLGLRGNNITKPPWEALRSLIS-----LNYLDLDYNLIQNLTKNAFGYLPVLFELGLSNNLIHNVSVEAFDGLLQLQKLDLSQNNITYIPPGAFKGMSALYDINISFNKIQHLQNRTHGLFEDTLSLRKIELSHNEI 732 HSP 2 Score: 89.3521 bits (220), Expect = 4.209e-18 Identity = 147/644 (22.83%), Postives = 243/644 (37.73%), Query Frame = 0
Query: 179 TELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFA-------------CGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTS--------PCPFPCSCSEGVVDCSRRG--VKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQL-----KTVPSN--VPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIK--CIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSI------------------------THLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAK------CSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDP 760
T++ + N + + FK + + +DLS+N+I I +D F T +L N++ +SG L+ L ++ N I I N F +L L L NNI + F+ L +L+ + L N+ DL++ + N V S + ++ + + L S + L++ C++ + P P S E +D ++ G +K D K L L N + P L +L+ +L+ L+L N I + + +F + L LS+N + + GL L L L NNI I PGAF + L NI N IQ HL++R G E R+ I SH ++ K P + P + L N I L + + L L+ISNN+++ ++ + NLT L L IS N +K +F + +L + LH N +P A +++ L NPL+CDC+L+ ++ W+K+ G +K C P L+ + V + E C D+ + K +P
Sbjct: 362 TKINISHNQLPFLSSGLFKNCENMTFLDLSHNQIAHISEDAFNENTYATEFLLQYNKLTSMSGVPLASQKGLKFLNMSHNAIVDIPRNTFPKLYELFRLDFSYNNISIIGKSIFSSLLTLRHIDLSHNSLTKLDSGMFGKVPTLLDLDLSYN----KLNQVRRSVFGGLSSIRFVKLHHNQLQEIPSPPISLNTMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGNSIKAGRFDGLIVLKSLGLRGNNITKPPWEALRSLI-SLNYLDLDYNLIQNLTKNAFGYLPVLFELGLSNNLIHNVSVEAFDGLLQLQKLDLSQNNITYIPPGAFKGMSALYDINISFNKIQ----------------------------HLQNRT----------------HGLFEDTLSLRK-------IELSHNEISFITPKMFPESPWAPYRVKHIDLSYNLIHVLTKSILKGTKYLDYLNISNNRLNDVRKGILTNLTSLKILDISGNSLKEDIFNNGSFGNMPNLTVFKLHNNKFETLPVKALLLQSNLKVLDIRNNKIKEYYTEFTPRIKKGMELYYEGNPLMCDCSLRPIAYWLKSVGRIKGRSKPWEDAICESPPYLSGNSVASVLEERLVC--ADSLDALKFKLNP 947 HSP 3 Score: 68.9366 bits (167), Expect = 7.445e-12 Identity = 106/460 (23.04%), Postives = 182/460 (39.57%), Query Frame = 0
Query: 49 GLVDCSRANLDTFPRDIPYDSHSIDLSFNSITQFPDV-SEWLEYAQIRHIDVSHNFIECFDSNLVNH-KVEHVLELKHGRGCRSDEDLLTAGESICLDSRVNCPKECHCNDGIVDC-RDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCL----DLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTK----------ELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGR---LLRGLDGLTTLHLHNNNIHCISPGAF 488
G VD +R NL + D ID Q ++ E + I++SHN + S L + + L+L H + ED + + + ++ + + P + G L + N IV IP F KL L R+D S N I +I F+ L L + L N + ++ F +P+L L L+ NK+ + + F L + + L+ N +Q + + SL T+ C + + + P + E + + +S+ GR + L L N T P P ++ + + + I TK EL LS+N++ V SV F+ L L L+LS+N I+ I G+F+G +++ +++S N + ++ R L L + L +N I I+P F
Sbjct: 315 GTVDLARNNLTKVDYQLFSDLRFIDTISLQENQIKNIDKEAFSNLYLTKINISHNQLPFLSSGLFKNCENMTFLDLSHNQIAHISEDAFN--------------ENTYATEFLLQYNKLTSMSGVPLASQKGLKFLNMSHNAIVDIPRNTFPKLYELFRLDFSYNNISIIGKSIFSSLLTLRHIDLSHNSLTKLDSGMFGKVPTLLDLDLSYNKLNQVRRSVFGGLSSIRFVKLHHNQLQEIPSPPI----SLNTM---------DCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLG-NSIKAGRFDGLIVLKSLGLRGNNITKP---PWEALRSLISLNYLDLDY--NLIQNLTKNAFGYLPVLFELGLSNNLIHNV-SVEAFDGLLQLQKLDLSQNNITYIPPGAFKGMSALYDINISFNKIQHLQNRTHGLFEDTLSLRKIELSHNEISFITPKMF 740 HSP 4 Score: 52.373 bits (124), Expect = 9.702e-7 Identity = 64/255 (25.10%), Postives = 105/255 (41.18%), Query Frame = 0
Query: 446 IHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNV---PKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSN 697
IH L +S+ ++ + G L + L + L +H+ I IS G FD L S+ + + S N ++L + + Q+ LP EG L E + SH +++++ + V + +L L N + L F +L LDISNN+ S ++ + F L +L L IS+NEI IQ F+ LR+L L+ N I + ++ + L L N
Sbjct: 75 IHTLIISNCNIEKLYGSLFKLLT-IRILKIHDTPIKDISDGTFDQLS--------SSLEELHIQNSLLTRLSPSIKNLTSLKIL----FIENSQISYLP-----------EGVLNGMNSLEE-------LSISHGKIQSIGNKVFNDVRRLKKLSLFKNQLSSLPRDAFKSQGQLEFLDISNNQFSKLEAHYFPKLRKLLWLNISHNEIPRIQSRVFARNSLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDN 298
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Match: EMLSAG00000004789 (supercontig:LSalAtl2s:LSalAtl2s251:949705:953355:1 gene:EMLSAG00000004789 transcript:EMLSAT00000004789 description:"augustus_masked-LSalAtl2s251-processed-gene-9.0") HSP 1 Score: 105.145 bits (261), Expect = 6.454e-23 Identity = 160/655 (24.43%), Postives = 248/655 (37.86%), Query Frame = 0
Query: 171 PASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIR--------------LIPDDTFTGLPR--------LTSLVLYGNQIERISGSSF-YDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAF------ACGCDLAWLTDW-LQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTI--------RGRLLRGLD----------------GLTTLHLHNNNIHCISPGAFDLLPKLS----FTNIL-----------------------SNPIQCDCHMAWFGDWLRSQNNTSALATCA-------TPAHLRD----RQLQELPLPSFRCKG--------SEDEGCLEQHYCPRECVC------------------------TGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLC 701
P + P L L +N + S +FK++++L +D+S NEI L D +F L R L + L GN +ER+ F ++ L +L L SN+I IHPNAF L +SL N + + + + SL+ L++G+N + +L L +N +E G+ V ++ + +LHL ++ L R + I D+ + ++L N LD + VGLF LP L+ LN+S N I + F S+H L L HN++ I LL+ LD L L L NN I I F + +L FTN + NP CDC + W +R N L A ++R+ + L + S R + S D C+ + +C C+C + ++ C A L P+ +P +A+E+YL ND L F + L L ++++ I VI F+ L RL L + N I + + F L +L+ L L GN IS I + F + L L N L+
Sbjct: 243 PETFPSSLVILNLSENKLRS--DLSFKQMQYLENLDISKNEIANLHPSMLGNSVPGLLYLDLSFNRLSRISLRRLHQLQKVRLEGNVLERLDPEMFDMNMTELHVLDLTSNEIESIHPNAFINCPALEDVSLKKNKLTYFPSDAISKILSLKVLNIGRNRIIKLSNGTFPVQMKYLHSLILDENAIEEVGS----DVFMRLPNLKTLHLSKNRIQKL-------------------------YRNLFINNKDM----EVVKLDSNRLDDI--VGLFKGLPKLNWLNISNNAIDKFDW--FLVPNSLHWLXLKHNAIEEIGNYFSLEKEFSLLKSLDVSHNKLTFIGPKILPKNLQFLILRNNFISEIQMHTFIDMKELERVDLFTNQIKHLSKQSLQLSQDLRKRTEFYLGENPFVCDCDLEWL---IRINNEDDHLKNSAFSLNGPHNKTYIRNYPFVKDLDSISCLSERSESNQVIPLIQSTDFLCIYKTHCYSLCLCCDYDACDCEMTCPDHCRCYHDGSWSINIVDCHSAGLDQPPTAIPMDATEVYLQGNDFKKLTSHSFIGRKNLKILFLNSSNIEVILNNTFSGLRRLKILHLENNRITNLTGNEFQSLINLKALYLEGNRISHIHSKTFSYLKELEILRLHDNKLIT 855 HSP 2 Score: 99.7525 bits (247), Expect = 2.551e-21 Identity = 65/181 (35.91%), Postives = 102/181 (56.35%), Query Frame = 0
Query: 144 DSRVNCPKECHC-NDG-----IVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLD-NGTFNGLSSLQTLHLGKNAFACGCDLAWL 317
D + CP C C +DG IVDC L++ P +IP ATE+ L+ N+ + +F K L+ + L+++ I +I ++TF+GL RL L L N+I ++G+ F L +L+ L L N+I IH F+ L+ L +L L+DN + T + NGL SL+ L L +N + C D +W+
Sbjct: 707 DCEMTCPDHCRCYHDGSWSINIVDCHSAGLDQPPTAIPMDATEVYLQGNDFKKLTSHSFIGRKNLKILFLNSSNIEVILNNTFSGLRRLKILHLENNRITNLTGNEFQSLINLKALYLEGNRISHIHSKTFSYLKELEILRLHDNKLITFHFDIILNGLRSLKVLSLSRNKWNCS-DCSWI 886 HSP 3 Score: 83.9593 bits (206), Expect = 2.205e-16 Identity = 150/629 (23.85%), Postives = 246/629 (39.11%), Query Frame = 0
Query: 120 LELKHGRGCRSDEDLLTAG---ESICLD---SRV-NCP-KECHCNDGIVDCRDKKLERF---PASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSE-GVVDCSRRGVKII-PSDIPK--TTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFE-GARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCIS-----------------------PGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTV-PSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWL 708
LE C + E+LLT G +S L+ +R+ + P K HC+ +D RD + P + EL L +N + + + F L +++LS N I I ++ L LTSL L N I +S S+F L L + L+ N++ I P F L +L+L +N +++ + +F + L+ L + KN ++A L + N V + +L R + LH + + L P F + +E V+D + ++ I P+ +++ L N L PS + +L +L LN+ RN I + G+F + +H L L N++ + + L L TLHL N I + G F LPKL++ NI +N I DW N+ L L+ ++E+ + LE+ + + + SH +L + P +PK L L N I +++M F ++ L R+D+ N+I HL SL + D T LG NP +CDC+L+WL
Sbjct: 87 LEXIAFENCENMENLLTEGYLRQSFNLEIAGARLRHFPGKHLHCSLNYLDLRDNDIRNLNLDPFCSKNSLKELNLARNVLKVLHNEVFLGWYSLSKLNLSGNRINSIEENALQPLQGLTSLDLSQNLISVLSPSTFIHLKRLATINLSKNRLVGISPETFP--SSLVILNLSENKLRS--DLSFKQMQYLENLDISKN------EIANLHPSMLGNSVPGLLYLDLSFNRLSRISLRRLH--QLQKVRLEGNVLERLDPEMFDMNMTELHVLDLTSNEIESIHPNAFINCPALEDVSLKKNKLTYFPSDAISKIL-SLKVLNIGRNRIIKLSNGTFPVQMKYLHSLILDENAIEEVGSDVFMRLPNLKTLHLSKNRIQKLYRNLFINNKDMEVVKLDSNRLDDIVGLFKGLPKLNWLNISNNAIDK-------FDWFLVPNSLHWLX-------LKHNAIEEIG----------NYFSLEKEFSLLKS------LDVSHNKLTFIGPKILPKNLQFLILRNNFISEIQMHTFIDMKELERVDLFTNQIK-----------------------------------HLSKQSLQLSQ----------DLRKRTEFYLGENPFVCDCDLEWL 627
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Match: EMLSAG00000006314 (supercontig:LSalAtl2s:LSalAtl2s343:642:15163:-1 gene:EMLSAG00000006314 transcript:EMLSAT00000006314 description:"maker-LSalAtl2s343-augustus-gene-0.12") HSP 1 Score: 103.99 bits (258), Expect = 1.958e-22 Identity = 85/358 (23.74%), Postives = 141/358 (39.39%), Query Frame = 0
Query: 772 CRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCT-----------VPENGRYV-----------CDCPPGYSGLRCEFEIDECESNPCQNNATCF-DEVARFRCDCPEGYSGETC-SAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVY------VGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDD 1099
HH + + + G N D C NPC H G C+ + E+ + C C P Y+G RC+ + + C NPC C + F+C CP G+ C + + D C + + C NGGSC +++ C C PG+ G C ++D C + C NGG C+ Y C C F G CE L++ FD+ ++A PL++ +I++ T + + +LVY GR++ LA+EL +GR + S+ V+ + KI++G +++ + +L ++D
Sbjct: 3638 VHGHHQTKSYLQSIGQFDGISL--NFDPCSNNPCLHFGQCSSRKIFVNHSTSIVESSDTIFNSPLLLQDVRCICTPEYAGSRCQIQKNPCNPNPCLEGGNCVVRSESSFQCLCPPLRGGDLCETTQTDVC--SPNPCFNGGSCQKGXRDSFFCRCRPGYQGDRCERAVDSCLRNPCLNGGVCISQKPDYRCRCPDNFYGNRCE-------------------------------------------------LSTFGFDEL-SFAAFPPLDSN-TNDISITFATTKANSLLVYNHGEVSGGRSDFLALELVRGRPKFSWGAARTAVTILELPRKINNGRWYKVTATRNNRVASLSIED 3940 HSP 2 Score: 88.9669 bits (219), Expect = 6.950e-18 Identity = 45/126 (35.71%), Postives = 60/126 (47.62%), Query Frame = 0
Query: 750 TPQEIVAKC----DPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEK-NIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETC 870
TP+ ++C +PC+ +PC C F C C P G +CE D C NPC +GG+C + C C PGY G RCE +D C NPC N C + +RC CP+ + G C
Sbjct: 3710 TPEYAGSRCQIQKNPCNPNPCLEGGNCVVRSESSFQCLCPPLRGGDLCETTQTDVCSPNPCFNGGSCQKGXRDSFFCRCRPGYQGDRCERAVDSCLRNPCLNGGVCISQKPDYRCRCPDNFYGNRC 3835 HSP 3 Score: 85.5001 bits (210), Expect = 8.777e-17 Identity = 59/185 (31.89%), Postives = 77/185 (41.62%), Query Frame = 0
Query: 754 IVAKCDPCHSSPCQNKATCRA-----HHGLEFI-----------------CDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCE-FEIDECESNPCQNNATCFDEVA-RFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVS 914
I DPC ++PC + C + +H + C C P Y G+ C+ + C NPC GG C V + C CPP G CE + D C NPC N +C F C C GY G+ C +D C+ + CLNGG C+ S Y C C F G C +S
Sbjct: 3657 ISLNFDPCSNNPCLHFGQCSSRKIFVNHSTSIVESSDTIFNSPLLLQDVRCICTPEYAGSRCQIQKNPCNPNPCLEGGNCVVRSESSFQCLCPPLRGGDLCETTQTDVCSPNPCFNGGSCQKGXRDSFFCRCRPGYQGDRCERAVDSCLR--NPCLNGGVCI-SQKPDYRCRCPDNFYGNRCELS 3838
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Match: EMLSAG00000011270 (supercontig:LSalAtl2s:LSalAtl2s772:2760:23028:-1 gene:EMLSAG00000011270 transcript:EMLSAT00000011270 description:"maker-LSalAtl2s772-snap-gene-0.12") HSP 1 Score: 102.834 bits (255), Expect = 4.339e-22 Identity = 69/211 (32.70%), Postives = 96/211 (45.50%), Query Frame = 0
Query: 764 SPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTV-PENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHG 973
S C N C C C PG+ G CE+ I A +PC +GGTC + + + C CP ++G RCE + + + C N+ TC + C CP ++G C CLNGG+ C C PG GP C ++ C+ C+N GTC+ NG C CT + G C + I SH + SPC+ H C +G
Sbjct: 4144 SLCVNNGVCYLDEKNSTKCKCRPGFSGDRCERCISA--KDPCENGGTCLINAYDEKPRCVCPETFTGERCETSMCDSQIGSCLNDGTCIVQEGIHNCLCPPDFAGPNCQT-----FKCDRYCLNGGTPNVRVPGQCRCDCRPGTGGPRCQITR--CKKLECKNSGTCILLNGREVCRCTERYGGLDCSVR-IPSHAH---SPCEFHRCLNG 4341 HSP 2 Score: 97.0561 bits (240), Expect = 2.483e-20 Identity = 92/309 (29.77%), Postives = 132/309 (42.72%), Query Frame = 0
Query: 756 AKCDPCHSSPCQN-KATCRAHHGLEFICDCVPGYYGTVCEKN------------IDACYGNPCSHGGTCTVPE------------------NGRY--------VCDCPPGYSGLRCEFEIDECESNPCQNNATCF-DEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTY-TCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAG---------KY----------------CELEPIISHMYPQSSPCQQHECKH-GTCFMPKDSKDYVCKCDPGYTGKYC 997
A +PC SPC + + C ++ C+CV GY T ++N DAC G C H G C + + +G+Y C G+ G RCE I+ CES C NN C+ DE +C C G+SG+ C + C++A D C NGG+CL +A + C C F+G C S+ D + C N GTC+ G ++C C P+FAG +Y C+ P Q + C++ ECK+ GTC + + VC+C Y G C
Sbjct: 4021 AISNPCFLSPCSDPNSICIMLSPEKYKCECVDGYLSTKRDQNGSFCVKKEEGFKSDACEGIQC-HTGKCILRQFKKSSELEAHCKCTNPFYSGKYCDTYICTGYCLNGAGFEGDRCETRIESCES-LCVNNGVCYLDEKNSTKCKCRPGFSGDRC----ERCISAKDPCENGGTCLINAYDEKPRCVCPETFTGERCETSMCDSQIGSCLNDGTCIVQEGIHNCLCPPDFAGPNCQTFKCDRYCLNGGTPNVRVPGQCRCDCRPGTGGPRCQITRCKKLECKNSGTCILLNGRE--VCRCTERYGGLDC 4321
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Match: EMLSAG00000009983 (supercontig:LSalAtl2s:LSalAtl2s652:91522:194127:1 gene:EMLSAG00000009983 transcript:EMLSAT00000009983 description:"maker-LSalAtl2s652-augustus-gene-2.26") HSP 1 Score: 102.064 bits (253), Expect = 5.918e-22 Identity = 135/544 (24.82%), Postives = 221/544 (40.62%), Query Frame = 0
Query: 164 DKKLERFPAS---IPHGATELRLEQNNIVSIPPKAFK-KLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPI-QCDCHMAWFGDWLRSQN-NTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLC 701
D FP S I T L L QN + ++ + + +L L +++S NE ++ TF+ +P L L + NQ+ I +F+ +L +L L+ N + + P+ F+R L + L N ++ L F L +L+ + L N L+ DW +P + ++ L + ++ S S P K EL LS N L V+ + F AL+TLNL N ISVIE GSF G + L L +N L + L L L+ LHL +N I I P AF L L + ++ N + + + M + LRS + + +++ ++ L + + R + + E R + ++ L+ + + + L N I L A LQ+ L ++NN+I+ I F+ L L +S+N I+ I+ AF G L L L+ I I +A DD ++ L + N LL
Sbjct: 170 DNHFSIFPMSAIKILKKLTLLNLSQNKLSNVSFEGYNNRLDSLLNLEMSFNEFEILHMQTFSSMPNLKHLDVSHNQLHFIQEDTFFSQSNLAILDLSYNSLAELPPDIFHRCHRLTFVDLSHNRLKGLSQ-VFKDLIALEEVALNNNML-----LSLGQDWFNNSP-----------------NLKTIRLQHNIIFEIQSESLQ-------PL----------------------KRLSELHLSFNFLSVITRMD-FRYNVALNTLNLDNNHISVIETGSFRGVPRLRELRLQNNVLKEVSRELFSSLAQLSELHLQDNKITKIEPQAFSGLALLQYLDLRGNKLTKVEDVMMRYPSTLRSMYLDGNRISSFHKETFSGQTTVEVLSISNNRLEIITPKMFEELINLFRLTLSNNRIVSIEDYALQGL-----HQVRVMDLSNNRIVKLTNATLKGLQQAETLSLNNNRINSISNEAFSGFHHLKELDLSHNRIRMIRNGAFRGCLTLHTLRLNDAGIRFIAPNALDDFKKLSVLEMSQNQLLI 655 HSP 2 Score: 90.5077 bits (223), Expect = 2.045e-18 Identity = 135/508 (26.57%), Postives = 206/508 (40.55%), Query Frame = 0
Query: 181 LRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQ---RKKISSLHLGRSNCLDLSSASCN---WTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTT--KELQLSDNVLDVVPSVG---LFNLLPALSTLNLSRNFISV-IEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSIS 676
L L N IVSI A + L +R +DLSNN I + + T GL + +L L N+I IS +F L+ L L+ N+IR I AF LH L L D I+ + + L L + +N + LT +P+ + + L R K +L G SN + CN + + + + + S + K+ K +EL L N VP LFNL LNLS N + ++E SF+ + +LDLS NS+ I R + GL L++ NN+ F +P + ++++SN +NT A R L C G L++ R + + T + S L + E +L L N I+ LD+S N++++I PY F+ LT+L L +SYN+I + F+GL L L+L N +S
Sbjct: 526 LTLSNNRIVSIEDYALQGLHQVRVMDLSNNRIVKLTNATLKGLQQAETLSLNNNRINSISNEAFSGFHHLKELDLSHNRIRMIRNGAFRGCLTLHTLRLNDAGIRFIAPNALDDFKKLSVLEMSQNQLLIPEAIHRLTPNPGPSPLSSLKVLVLSHNDLSNATRSKDYTLS-GLSNLETIELEGCNLSKFRKEFLWENTKLKTIRLGSNKLEKLEEEIFSKQVFLEELHLDRNKFKSVPREATRYLFNL----RLLNLSGNMLRENVDESSFDNVAGLKLLDLSGNSIKHIPERTFHNISGLLQLNISNNSFLGFQSRTF--VPLVQLSSLISN------------------HNTLPFIPIAIQGLRSVRTL---------CVGGNPLERLKEIPESRAVMHSVTTAQISGTNLSIL------ETGDLGLFPNLIN---------------LDLSRNQLTMIAPYSFSKLTQLLRLDLSYNQILQLTSERFAGLESLSTLNLSYNLLS 978 HSP 3 Score: 79.7221 bits (195), Expect = 4.644e-15 Identity = 151/645 (23.41%), Postives = 239/645 (37.05%), Query Frame = 0
Query: 153 CHCNDGIVDCRDKKLERFPASIPHG---ATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFA-----CGCDLAWLTDW-LQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPC--PFPCSCSEGVVDCSRRGVKIIPSDIPKTTKE-LQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHI------------------------------------------------LDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSN------------PIQCDCHMAWFGDWLRSQNNTSALA-----TCATPAHLRDRQLQELPLPSFRCK-------------GSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDI--------DDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPL 699
N I+D L P I H T + L N + + + FK L L + L+NN + + D F P L ++ L N I I S L L L L+ N + I F L+ L+L +N+I ++ G+F G+ L+ L L N LA L++ LQ N + K++ + S L L + LDL +P + +D +R + +TT E L +S+N L+++ + +F L L L LS N I IE+ + +G + + LDLSHN + IR RG L TL L++ I I+P A D KLS + N P ++ + S N+ S T + ++L +L+ L FR + GS LE+ ++ + + K+V P+EA+ ++ + ++++ + F ++ L LD+S N I I F N++ L L IS N Q F L L L + N++ IP A S+ L +G NPL
Sbjct: 256 SQSNLAILDLSYNSLAELPPDIFHRCHRLTFVDLSHNRLKGLS-QVFKDLIALEEVALNNNMLLSLGQDWFNNSPNLKTIRLQHNIIFEIQSESLQPLKRLSELHLSFNFLSVITRMDFRYNVALNTLNLDNNHISVIETGSFRGVPRLRELRLQNNVLKEVSRELFSSLAQLSELHLQDNKI----------TKIEPQAFSGLALLQ--YLDLRGNKLTKVEDVMMRYPSTLRSMYLDGNRISSFHKETFSGQTTVEVLSISNNRLEII-TPKMFEELINLFRLTLSNNRIVSIEDYALQGLHQVRVMDLSNNRIVKLTNATLKGLQQAETLSLNNNRINSISNEAFSGFHHLKELDLSHNRIRMIRNGAFRGCLTLHTLRLNDAGIRFIAPNALDDFKKLSVLEMSQNQLLIPEAIHRLTPNPGPSPLSSLKVLVLSHNDLSNATRSKDYTLSGLSNLETIELEGCNLSKFRKEFLWENTKLKTIRLGSNKLEKLEEEIFSKQVFLEE--LHLDRNKFKSV----PREATRYLFNLRLLNLSGNMLRENVDESSFDNVAGLKLLDLSGNSIKHIPERTFHNISGLLQLNISNNSFLGFQSRTFVPLVQLSSLISNHNTLPFIP-IAIQGLRSVRTLCVGGNPL 879 HSP 4 Score: 70.8626 bits (172), Expect = 2.125e-12 Identity = 124/538 (23.05%), Postives = 206/538 (38.29%), Query Frame = 0
Query: 179 TELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPS---DIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSL---TTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHY---CPRECVCTGTVIRCSHAQLKTVPSNVPKEASE-------LYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISM---IPNSAFDDHNSITHLALGSN 697
+EL L+ N I I P+AF L L+ +DL N++ + D L S+ L GN+I +F ++++L +++N++ I P F L L L+L +N I ++++ GL ++ + L N + A L Q ET + + S H + LDLS F + + + G++ I D K L++S N L + ++ P S L+ S+ +L LSHN L T + L GL L T+ L C+++ F +N T L +L++L F + +E L+++ PRE +R + + NV + + + L L N I + F ++ L +L+ISNN + F L +LS+LI ++N + I P A GL +R L + GN + IP S H+ T G+N
Sbjct: 428 SELHLQDNKITKIEPQAFSGLALLQYLDLRGNKLTKVEDVMMRYPSTLRSMYLDGNRISSFHKETFSGQTTVEVLSISNNRLEIITPKMFEELINLFRLTLSNNRIVSIEDYALQGLHQVRVMDLSNNRIVKLTN-ATLKGLQQ---AETLSLNNNRINSISNEAFSGFHHLKE--LDLSHNRIRMIRNGAFRGCLTLHTLRLNDAGIRFIAPNALDDFKKLSVLEMSQNQLLIPEAIHRLTPNPGPSPLS------------------SLKVLVLSHNDLSNATRSKDYTLSGLSNLETIELEG------------------------------CNLSKFRKEFLWEN------TKLKTIRLGSNKLEKLEEEIFSKQVFLEELHLDRNKFKSVPREATRYLFNLRLLNLSGNMLRENVDESSFDNVAGLKLLDLSGNSIKHIPERTFHNISGLLQLNISNNSFLGFQSRTFVPLVQLSSLISNHNTLPFI-PIAIQGLRSVRTLCVGGNPLERLKEIPESRAVMHSVTTAQISGTN 904
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Match: EMLSAG00000010969 (supercontig:LSalAtl2s:LSalAtl2s736:33395:37479:1 gene:EMLSAG00000010969 transcript:EMLSAT00000010969 description:"maker-LSalAtl2s736-augustus-gene-0.4") HSP 1 Score: 100.523 bits (249), Expect = 1.074e-21 Identity = 148/555 (26.67%), Postives = 231/555 (41.62%), Query Frame = 0
Query: 180 ELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNA-----FACGCDLAWL-TDWLQKNPVETSGARCETPVKLQRKKISSLH---LGR---SNC-----LDLSSASCNWTSPCPF--PCSCSEGVVDCSR-RGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSN--------PIQC---DCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVI-RCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSA---FDDHNSITHLALGSNPL 699
+L L +N + S+P AFK L +D+S+N + F L +L L + N+I RI F L++L LN+NKI + N+ + L L L DN I ++ G F +S + T+ L +N + DL ++ T LQ+N ++ T + L + IS LG NC LDLS + F +E ++ ++ + +P K K L +S N + +P F L + L+ S N IS+I + F S+ +DLSHNSLT + +L + L + L N ++ + F L + F + N PI DC S NN + P + + L EL L + S + G + + G I + L+++ + + + LD N I +L F L L L +SNN I+ I F L +L L +S N I I P AF G+ L ++L N I + N F+D SI + L N +
Sbjct: 196 KLSLFKNQLSSLPRDAFKSQGQLEFLDISHNHFSKLEPHYFPNLRKLLWLNISHNEIPRIQSRVFARNSLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDNFIPSVGRGAFRAVSRIGTVDLARNNLTKVDYQLFSDLRFIDTISLQQNQIKNIDKEAFTNLYLTKINISHNQIPFLGSGLFKNCENMTFLDLSHNQIVHIAEDAFNENTYATEFLLQYNKLTSMSGVPLTYQKGLKVLNVSHNEIVDIPK-NTFPKLYEVFRLDFSHNNISIIGKSVFSTLLSLRHIDLSHNSLTKLDSGMLGKVPTLLDIDLSYNKLNRVRRSVFGGLSSIRFVKLHHNELNEVPAPPISLNAMDC----------SYNNIKEIK-GRQPWPVMN-SLLELNLDYNQLGDSLEAGRFDGLIVLKSIGLRGNNISKPPWEALRSL-----RSVNYIDLDDNHIRNLSRNAFGRLPILFELGLSNNSINNISVEAFGGLLQLQKLDLSQNNITYIPPGAFKGMSALYDINLSFNKIKHLQNRTHGLFEDTLSIRKIDLSHNQI 732 HSP 2 Score: 80.4925 bits (197), Expect = 1.503e-15 Identity = 137/565 (24.25%), Postives = 224/565 (39.65%), Query Frame = 0
Query: 149 CPKE----CHCNDG-----IVDCRDKKLERFPASIPHGAT---ELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLP-RLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDI---PKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQC-------DCHMAWFGDWLRSQNNTSALA-------TCATPAHLRDRQLQELP-LPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNV-PKEASELYLDV--NDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIP 679
CPK C C G ++C + + + T L + NI + FK L +R + + + I+ I D TF L L L + + + R+S S +L SL++L + +++I + N L L LS+ NI+++ FN + L+ L L KN + L R S G+ LD+S + P FP ++ S + I S + + L L+ N +D + + L + L L LS NFI + G+F I +DL+ N+LT + +L L + T+ L N I I AF L L+ NI N I +C F D S N +A T AT L+ +L + +P KG + V+ SH ++ +P N PK LD N+I + + FS L L +D+S+N ++ + + + L + +SYN++ ++ F GL +R + LH N ++ +P
Sbjct: 34 CPKRSIFPCECTKGSDEGIYLECSNTNIASLSVGLQQVKTLIHTLIINNCNIEKLYGNLFKPLT-IRILKIFDTPIKDISDGTFDQLSVSLEELHIKNSLLTRLS-PSIKNLTSLKILFIENSQISYLPEGVLNGLNSLKELSISHGNIKSIGKKVFNDVRQLKKLSLFKNQLSS----------------------------LPRDAFKSQ--GQLEFLDISHNHFSKLEPHYFPNLRKLLWLNISHNEIPRIQSRVFARNSLLRVLHLNANKIDRLDTNSLRGM-RFLRRLYLSDNFIPSVGRGAFRAVSRIGTVDLARNNLTKVDYQLFSDLRFIDTISLQQNQIKNIDKEAFTNL-YLTKINISHNQIPFLGSGLFKNCENMTFLDL--SHNQIVHIAEDAFNENTYATEFLLQYNKLTSMSGVPLTYQKGLK-------------------VLNVSHNEIVDIPKNTFPKLYEVFRLDFSHNNISIIGKSVFSTLLSLRHIDLSHNSLTKLDSGMLGKVPTLLDIDLSYNKLNRVRRSVFGGLSSIRFVKLHHNELNEVP 543 HSP 3 Score: 62.7734 bits (151), Expect = 4.515e-10 Identity = 90/355 (25.35%), Postives = 139/355 (39.15%), Query Frame = 0
Query: 179 TELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKII----PSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILD------------------------LSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQ 505
T++ + N I + FK + + +DLS+N+I I +D F T +L N++ +SG L++L ++ N+I I N F +L + L NNI + F+ L SL+ + L N+ D + L K P + R+ + G + + N + P P S +DCS +K I P + + EL L N L G F+ L L ++ L N IS + RS++ +D LS+NS+ I GL L L L NNI I PGAF + L N+ N I+
Sbjct: 362 TKINISHNQIPFLGSGLFKNCENMTFLDLSHNQIVHIAEDAFNENTYATEFLLQYNKLTSMSGVPLTYQKGLKVLNVSHNEIVDIPKNTFPKLYEVFRLDFSHNNISIIGKSVFSTLLSLRHIDLSHNSL-TKLD----SGMLGKVPTLLDIDLSYNKLNRVRRSV----FGGLSSIRFVKLHHNELNEVPAP-PISLNAMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGDSLEAGRFDGLIVLKSIGLRGNNISKPPWEALRSLRSVNYIDLDDNHIRNLSRNAFGRLPILFELGLSNNSINNISVEAFGGLLQLQKLDLSQNNITYIPPGAFKGMSALYDINLSFNKIK 706 HSP 4 Score: 60.8474 bits (146), Expect = 2.146e-9 Identity = 75/259 (28.96%), Postives = 118/259 (45.56%), Query Frame = 0
Query: 59 DTFPRDIPYDSHSIDLSFNSITQFPDVSEWLEYAQIRHIDVSHNFIECFDSNLVNHKVEHVLEL-----KHGRGCRSDEDLLTAGESICLD-SRVNCPKECHCNDGIVDCRDKKLE----RFPASIPHGATELRLEQNNIV-SIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKN 306
+TFP+ Y+ +D S N+I+ S + +RHID+SHN + DS ++ KV +L++ K R RS L++ + L + +N + +DC ++ R P + + EL L+ N + S+ F L L+ I L N I P + L + + L N I +S ++F LP L L L++N I I AF L L L L NNI + G F G+S+L ++L N
Sbjct: 449 NTFPK--LYEVFRLDFSHNNISIIGK-SVFSTLLSLRHIDLSHNSLTKLDSGMLG-KVPTLLDIDLSYNKLNRVRRSVFGGLSSIRFVKLHHNELNEVPAPPISLNAMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGDSLEAGRFDGLIVLKSIGLRGNNISKPPWEALRSLRSVNYIDLDDNHIRNLSRNAFGRLPILFELGLSNNSINNISVEAFGGLLQLQKLDLSQNNITYIPPGAFKGMSALYDINLSFN 703 HSP 5 Score: 59.3066 bits (142), Expect = 6.169e-9 Identity = 100/443 (22.57%), Postives = 169/443 (38.15%), Query Frame = 0
Query: 49 GLVDCSRANLDTFPRDIPYDSHSIDLSFNSITQFPDV-SEWLEYAQIRHIDVSHNFIECFDSNLVNH-KVEHVLELKHGRGCRSDEDLLTAGESICLDSRVNCPKECHCNDGIVDC-RDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNG---------TFNGLSSL---QTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKIS-----SLHLGRS-NCLDLSSASCNWTSPCPF---PCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIE---EGSFEGARSIHILDLSHNSLTTIRGRLL 464
G VD +R NL + D ID Q ++ E + I++SHN I S L + + L+L H + ED + + + ++ + + P + G L + N IV IP F KL + R+D S+N I +I F+ L L + L N + ++ +P+L + L+ NK+ + + F L + + L+ N + + ++N + + Q + + D L D L+ + G + L+ IS +L RS N +DL S F P G+ + S + + ++L LS N + +P G F + AL +NLS N I ++ G FE SI +DLSHN + I ++
Sbjct: 315 GTVDLARNNLTKVDYQLFSDLRFIDTISLQQNQIKNIDKEAFTNLYLTKINISHNQIPFLGSGLFKNCENMTFLDLSHNQIVHIAEDAFN--------------ENTYATEFLLQYNKLTSMSGVPLTYQKGLKVLNVSHNEIVDIPKNTFPKLYEVFRLDFSHNNISIIGKSVFSTLLSLRHIDLSHNSLTKLDSGMLGKVPTLLDIDLSYNKLNRVRRSVFGGLSSIRFVKLHHNELNEVPAPPISLNAMDCSYNNIKEIKGRQPWPVMNSLLELNLDYNQLGDSLEAGRFD--GLIVLKSIGLRGNNISKPPWEALRSLRSVNYIDLDDNHIRNLSRNAFGRLPILFELGLSNNSINNISVEAFGGLLQLQKLDLSQNNITYIPP-GAFKGMSALYDINLSFNKIKHLQNRTHGLFEDTLSIRKIDLSHNQIPFITPKMF 740 HSP 6 Score: 53.9138 bits (128), Expect = 2.941e-7 Identity = 52/182 (28.57%), Postives = 80/182 (43.96%), Query Frame = 0
Query: 526 LATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQH---YCPRECVCTG----TVIRCSHAQLKTVPSNV---PKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSN 697
L+ H+++ L L PS + S +E Y P E V G + SH +K++ V ++ +L L N + L F +L LDIS+N S ++P+ F NL +L L IS+NEI IQ F+ LR+L L+ N I + ++ + L L N
Sbjct: 119 LSVSLEELHIKNSLLTRLS-PSIKNLTSLKILFIENSQISYLP-EGVLNGLNSLKELSISHGNIKSIGKKVFNDVRQLKKLSLFKNQLSSLPRDAFKSQGQLEFLDISHNHFSKLEPHYFPNLRKLLWLNISHNEIPRIQSRVFARNSLLRVLHLNANKIDRLDTNSLRGMRFLRRLYLSDN 298
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Match: EMLSAG00000002202 (supercontig:LSalAtl2s:LSalAtl2s140:140781:148847:-1 gene:EMLSAG00000002202 transcript:EMLSAT00000002202 description:"maker-LSalAtl2s140-augustus-gene-1.15") HSP 1 Score: 90.1225 bits (222), Expect = 2.333e-18 Identity = 108/393 (27.48%), Postives = 160/393 (40.71%), Query Frame = 0
Query: 181 LRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTF-TGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNV---LDVVPSVGLFNLLPALSTLNLSRNFISVIEEGS---FEGARSIHILDLSHNSLTTIRGRLLR-GLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSAL----ATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQ 561
L L N++V I + L LR + LS N + +P F +P L L L N+ E+I G SF+ L L L L N + + AF LE + L L N IQ+++ G GL +L+TL L N + AW E G E +K S L L R ++ +PS EL L DN+ +D S +F +P + L+L N +S E S F+G + L LS N + +I + GL+ L + L N I I + L KL I S CDC++ W +++ N T + A CA P+ L+ R++ +P+ F C+ L+Q
Sbjct: 169 LNLRSNSLVQIQKGSLYGLNHLRELLLSRNSLVALPKGLFFDSVPSLKFLDLSKNRFEKIEGLSFHGLKELLTLKLKKNSVEYLSDGAFFGLEKIRHLYLDRNGIQSVEKGWLYGLRTLETLSLSHNKIDYIAEDAW----------EFLGVLRELNLK-------------SXLLQL-----------------------LERDSLRSLPS-----LTELYLQDNLISHIDDSSSGSVFEEVPLIQILSLDGNSLSHTIEDSDSPFQGLSHLRKLTLSRNKIKSIGELGFKGGLNSLEEIDLRANIISTIHEKSIKHLNKLKALKIDSKSFLCDCYLRWLPEFI---NLTHVMGFNQAVCAHPSSLKGRRVISVPIEYFTCEDFPKPYILQQ 507 HSP 2 Score: 83.5741 bits (205), Expect = 2.463e-16 Identity = 128/506 (25.30%), Postives = 188/506 (37.15%), Query Frame = 0
Query: 265 PNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKN--AFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSL-HLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAW-FGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLD---VNDIDDLEMAH-FSHLQRLSRLDISNNKISVI---KPYVFANLTRLSTLIISYNEIKCIQPHAF-SGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEP-GIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAK 757
P F RLE L L S NNI TL + FN L+ L+L KN + +LT ++T R + V++Q+ + L HL +EL LS N L +P F+ +P+L L+LS+N IE SF G + + L L NS+ + GL+ + L+L N I + G L L ++ N I AW F LR N S L QL E L+++PS +ELYL ++ IDD F + + L + N +S F L+ L L +S N+IK I F GL+ L + L N IS I + N + L + S LCDC L+WL +++ ++ A C++P SL V++ P F C ED P+ + +
Sbjct: 112 PFPFPRLENLDLRS---NNISTLKDLHFNLFPHLKVLNLAKNRILYLTPNVFQYLT------LLQTLNLRSNSLVQIQKGSLYGLNHL------------------------------------------------RELLLSRNSLVALPKGLFFDSVPSLKFLDLSKNRFEKIEGLSFHGLKELLTLKLKKNSVEYLSDGAFFGLEKIRHLYLDRNGIQSVEKGWLYGLRTLETLSLSHNKIDYIAEDAWEFLGVLRELNLKSXLL-----------QLLE------------------------------------RDSLRSLPS-----LTELYLQDNLISHIDDSSSGSVFEEVPLIQILSLDGNSLSHTIEDSDSPFQGLSHLRKLTLSRNKIKSIGELGFKGGLNSLEEIDLRANIISTIHEKSIKHLNKLKALKIDSKSFLCDCYLRWLPEFINLTHVMGFNQAVCAHPSSLKGRRVISVPIEYFTC--EDFPKPYILQ 506 HSP 3 Score: 56.6102 bits (135), Expect = 3.940e-8 Identity = 42/117 (35.90%), Postives = 58/117 (49.57%), Query Frame = 0
Query: 582 LKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSA-FDDHNSITHLALGSN 697
LK P P+ L L N+I L+ HF+ L L+++ N+I + P VF LT L TL + N + IQ + GL+HLR L L NS+ +P FD S+ L L N
Sbjct: 108 LKNGPFPFPR-LENLDLRSNNISTLKDLHFNLFPHLKVLNLAKNRILYLTPNVFQYLTLLQTLNLRSNSLVQIQKGSLYGLNHLRELLLSRNSLVALPKGLFFDSVPSLKFLDLSKN 223
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Match: gi|33302576|sp|P24014.2|SLIT_DROME (RecName: Full=Protein slit; Short=dSlit; Contains: RecName: Full=Protein slit N-product; Contains: RecName: Full=Protein slit C-product; Flags: Precursor) HSP 1 Score: 1025.39 bits (2650), Expect = 0.000e+0 Identity = 506/971 (52.11%), Postives = 661/971 (68.07%), Query Frame = 0
Query: 148 NCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNC----LDLS-SASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDECESN-PCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPK-DSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEGAN 1111
+CP C C DGIVDCR+K L P ++P TELRLEQN I +PPK+F + LRRIDLSNN I I D +GL +LT+LVLYGN+I+ + F L SLQLLLLN+N+I CI +AF L L LLSLYDNNIQ+L NGTF+ + S++T+HL KN F C C+L WL D+L KNP+ETSGARCE+P ++ R++I SL + C L + S C S CP C C VDC+ RG+K IP DIP T EL L+DN L + S GLF LP L L L RN ++ IE +FEGA I L L N + I ++ GL L TL+L++N I C+ PG+F+ L L+ N+ SNP C+CH+AWF +WLR ++ A C P+ +RD Q+++LP F+C EGCL YCP C CTGTV+RCS QLK +P +P E SELYL+ N+I+ + HL+ L+RLD+SNN+I+++ Y FANLT+LSTLIISYN+++C+Q HA SGL++LR+LSLHGN ISM+P +F+D S+TH+ALGSNPL CDC LKW S W+K Y+EPGIA+C+ P+ + + L+L+TP SSF C+ +I+AKC+ C PCQN+A C A E+ C C PGY+G CE IDACYGNPC + TCTV E GR+ C C PGY+G RCE ID+C CQNNATC D V ++C+C G+SGE C KI C + C NG C+ + Y+C C GF G +C+ ++DDC++HMCQNGGTC+DG Y C C ++ GKYCE +IS MYPQ+SPCQ HECKHG CF P DY+C+C PGYTGK+CEYLTS+SF ++ ++EPL T+P AN+T+ + E +G+L+Y G++ HLAVELF GRIRVSYDVGN+PVSTM+S+E ++DG H +++LA KN TLRVD G +RSI+NEG+N
Sbjct: 318 SCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAARCGAPSKVRDVQIKDLPHSEFKCSSENSEGCLGDGYCPPSCTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEIEQIHYERIRHLRSLTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCGLKWFSDWIKLDYVEPGIARCAEPEQMKDKLILSTPSSSFVCRGR-VRNDILAKCNACFEQPCQNQAQCVALPQREYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEIKCQNNATCIDGVESYKCECQPGFSGEFCDTKIQFCSPEFNPCANGAKCMDHFTH-YSCDCQAGFHGTNCTDNIDDCQNHMCQNGGTCVDGINDYQCRCPDDYTGKYCEGHNMISMMYPQTSPCQNHECKHGVCFQPNAQGSDYLCRCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNHPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSN 1286 HSP 2 Score: 318.161 bits (814), Expect = 2.987e-88 Identity = 193/670 (28.81%), Postives = 319/670 (47.61%), Query Frame = 0
Query: 138 GESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLR------------------------RIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSAS---CNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDL------------------------SHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSE-----DEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDT 750
G + + + CP+ C C VDC + L P I L L+ NN+ I F++L LR R+ L+NN ++ IP++ T L L + N I + F SL+ L L++N+I C+ +AF L L +L+L +NN+ +L + F GL L+ L L N FAC C L+WL+ +L+ RC++P +L+ + ++ LH C L+ + C + CP PC C++G+VDC + + +P +P T EL+L N + +P F+ L ++LS N IS I + G + + L L + N ++ IR R L L+ L L++NNI ++ G FD + + ++ NP CDC++ W D+L ++ A C +P + R+++ L F+C E C CP C C GT + C+ LK +P ++P +EL L+ N++ + F L L +L++ N+++ I+P F + + L + N+IK I F GLH L+ L+L+ N IS + +F+ NS+T L L SNP C+C+L W ++W++ + + G A+C P + + + P S FKC +E++
Sbjct: 62 GGGVGVITEARCPRVCSCTGLNVDCSHRGLTSVPRKISADVERLELQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFKGLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPFACDCHLSWLSRFLRSATRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGAENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPPKS-FSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLGSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPAMCHCEGTTVDCTGRGLKEIPRDIPLHTTELLLNDNELGRISSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASHIQELQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLNGGAARCGAPSKVRDVQIKDLPHSEFKCSSENS 730 HSP 3 Score: 70.0922 bits (170), Expect = 1.193e-10 Identity = 40/126 (31.75%), Postives = 62/126 (49.21%), Query Frame = 0
Query: 1124 GFECQCKPGFSGFYCNQKKDLSSSFQSDGSQREGKKGNKKNREGGRRVRNKRKKRCKKQKYRDYYIDNSGCRSKRPYKMAKCVSDAADSCVAKK-TKLRTIRFVCENGRTFNKEVEMIRKCGRIKK 1248
G++C+CK G G YC+Q + + + C+K++ R+YY +N CRS++P K AKCV + C A K + R +R VC N R + K ++++RKCG KK
Sbjct: 1401 GYQCKCKHGQRGRYCDQGEGSTEPPTVTAA-----------------------STCRKEQVREYYTEND-CRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKK 1502
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Match: gi|45477220|sp|O88280.1|SLIT3_RAT (RecName: Full=Slit homolog 3 protein; Short=Slit-3; AltName: Full=Multiple epidermal growth factor-like domains protein 5; Short=Multiple EGF-like domains protein 5; Flags: Precursor) HSP 1 Score: 816.224 bits (2107), Expect = 0.000e+0 Identity = 429/997 (43.03%), Postives = 609/997 (61.08%), Query Frame = 0
Query: 142 CLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNC------LDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHC------------------------ISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGR--YVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGT-CFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSI 1105
C + ++CP C C++ IVDCR K L PA++P G E+RLEQN+I SIP AF + K L+RID+S N+I I D F GL LTSLVLYGN+I I F L SLQLLLLN+NKI C+ N F L+ L+LLSLYDN +QT+ G F L S+QTLHL +N F C C L WL D+LQ NP+ETSGARC +P +L K+IS + + C + S+ C CP C C +VDCS + + IPS +P+ T +L+L+DN + V+ + G+F LP L +NLS N I + EG+F+GA + L L+ N L T+ GR+ RGL GL TL L +N I C ISPGAF L LS N+LSNP C+CHMAW G WLR + S C P L++ +Q++ + F C+G+E+ C CP +C C TV+RCS+ L T+P +PK+ +ELYL+ N + + S ++L+ +D+SNN IS++ + F+N++ LSTLI+SYN ++CI HAF+GL LR+L+LHGN IS +P +F+D S++HLALG NPL CDC+L+WLS+W+K Y EPGIA+CS P+S+A+ L+LTTP F+C+ IVAKC+ C SSPC+N TC ++ C C Y G C I+ C NPC HGGTC + E+ R + C CP G+ G RCE D+CE N C+N+ATC D + + C CP Y+GE C ID C+ + C + C+ S + C CVPG+SG C DDC +H C++G C+D Y+C C F+G +CE P + + Q+SPC Q+EC++G C + + ++ C+C PG+ G CE L +++F K +Y ++ +P ANI+++V T + +G+L+Y G N+ LA+EL++G +R+ YD + P +T++S E ++DG H ++++ + L L VD G +S+
Sbjct: 273 CNANSLSCPSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFIQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSSECFMDLVCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCEGNEENSCQLSPRCPEQCTCVETVVRCSNRGLHTLPKGMPKDVTELYLEGNHLTAVP-KELSTFRQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGINPLHCDCSLRWLSEWIKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGP-VDINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCQHGGTCHLSESHRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACVCPPNYTGELCDEVIDYCVPEMNLCQHEAKCI-SLDKGFRCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDAVNGYTCICPQGFSGLFCEHPPPMVLL--QTSPCDQYECQNGAQCIVVQ--QEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSL 1262 HSP 2 Score: 327.02 bits (837), Expect = 4.151e-91 Identity = 212/707 (29.99%), Postives = 334/707 (47.24%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPK------------------------AFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSA--SCNWTS-PCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRG------------------------LDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSED------EGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAH-FSHLQRLSRLDISNNKISVIKP------------------------YVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSP-CQNKATC 772
CP +C C+ VDC L P IP A L L++NNI I AF+ LK L R+ L+ N+++++P+ F P+LT L L NQI+ I +F + ++ L L++N I CI AF L L +L+L +NNI + +FN + ++TL L N C C LAWL+DWL++ C PV L+ ++ + C S +CN S CP CSCS +VDC +G+ IP+++P+ E++L N + +P+ G F L +++S+N IS I +F+G +S+ L L N +T I L G L L L L++N + IS G F L + ++ NP CDCH+ W D+L+ ++ A C++P L ++++ ++ FRC GSED C CP +C C GT++ CS+ +L +PS++P+ ++L L+ NDI LE F L L ++++SNN+I ++ +F L+ L TL++ N I C+ F+GL +R+LSL+ N I+ I AF S++ + L SNP C+C++ WL +W++ + I G +C P L + F C+ + + C SP C + TC
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFTGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTIGQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANSLSCPSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPA-GAFIQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSSECFMDLVCPEKCRCEGTIVDCSNQKLSRIPSHLPEYTTDLRLNDNDIAVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSGLKTLMLRSNLISCVNNDTFAGLSSVRLLSLYDNRITTISPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCEGNEE--------NSCQLSPRCPEQCTC 731 HSP 3 Score: 60.077 bits (144), Expect = 1.285e-7 Identity = 34/112 (30.36%), Postives = 51/112 (45.54%), Query Frame = 0
Query: 888 GSCLPSAVNTYTCSCVPGFSGPDCSVSLDD-CRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCE 998
G C ++ C C PG++GP C D C H C + GTC+ +Y C C + G C+ ++ C +C HG C + + Y C C PG++G +CE
Sbjct: 1341 GLCRSVEKDSVVCECHPGWTGPLCDQEAQDPCLGHSCSH-GTCVATGNSYVCKCAEGYEGPLCD------QKNDSANACSAFKCHHGQCHISDRGEPY-CLCQPGFSGNHCE 1444 HSP 4 Score: 57.7658 bits (138), Expect = 6.618e-7 Identity = 37/111 (33.33%), Postives = 54/111 (48.65%), Query Frame = 0
Query: 772 CRAHHGLEFICDCVPGYYGTVCEKNI-DACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDE---CESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCM 878
CR+ +C+C PG+ G +C++ D C G+ CSH GTC N YVC C GY G C+ + D C + C + + C C G+SG C + +PC+
Sbjct: 1343 CRSVEKDSVVCECHPGWTGPLCDQEAQDPCLGHSCSH-GTCVATGNS-YVCKCAEGYEGPLCDQKNDSANACSAFKCHHGQCHISDRGEPYCLCQPGFSGNHCEQE-NPCL 1450
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Match: gi|341942041|sp|Q9WVB4.2|SLIT3_MOUSE (RecName: Full=Slit homolog 3 protein; Short=Slit-3; Short=Slit3; Flags: Precursor) HSP 1 Score: 813.913 bits (2101), Expect = 0.000e+0 Identity = 427/997 (42.83%), Postives = 608/997 (60.98%), Query Frame = 0
Query: 142 CLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNC------LDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCIS------------------------PGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGR--YVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGT-CFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSI 1105
C + ++CP C C++ IVDCR K L PA++P G E+RLEQN+I SIP AF + K L+RID+S N+I I D F GL LTSLVLYGN+I I F L SLQLLLLN+NKI C+ N F L+ L+LLSLYDN +QT+ G F L S+QTLHL +N F C C L WL D+LQ NP+ETSGARC +P +L K+IS + + C + S+ C CP C C +VDCS + + IPS +P+ T +L+L+DN + V+ + G+F LP L +NLS N I + EG+F+GA + L L+ N L T+ GR+ RGL L TL L +N I C+S PGAF L LS N+LSNP C+CHMAW G WLR + S C P L++ +Q++ + F C G+E+ C CP +C C TV+RCS+ L +P +PK+ +ELYL+ N + + S ++L+ +D+SNN IS++ + F+N++ LSTLI+SYN ++CI HAF+GL LR+L+LHGN IS +P +F+D S++HLALG+NPL CDC+L+WLS+W+K Y EPGIA+CS P+S+A+ L+LTTP F+C+ IVAKC+ C SSPC+N TC ++ C C Y G C I+ C NPC HGGTC + EN R + C CP G+ G RCE D+CE N C+N+ATC D + + C CP Y+GE C ID C+ + C + C+ S + C CVPG+SG C + DDC +H C++G C+D Y+C C F+G +CE P + + Q+SPC Q+EC++G C + + ++ C+C PG+ G CE L +++F K +Y ++ +P ANI+++V T + +G+L+Y G N+ LA+EL++G +R+ YD + P +T++S E ++DG H ++++ + L L VD G +S+
Sbjct: 273 CNANSLSCPSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSSECFMDLVCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCVETVVRCSNRGLHALPKGMPKDVTELYLEGNHLTAVP-KELSAFRQLTLIDLSNNSISMLTNHTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPESMADRLLLTTPTHRFQCKGP-VDINIVAKCNACLSSPCKNNGTCSQDPVEQYRCTCPYSYKGKDCTVPINTCVQNPCEHGGTCHLSENLRDGFSCSCPLGFEGQRCEINPDDCEDNDCENSATCVDGINNYACLCPPNYTGELCDEVIDYCVPEMNLCQHEAKCI-SLDKGFRCECVPGYSGKLCETNNDDCVAHKCRHGAQCVDEVNGYTCICPQGFSGLFCEHPPPMVLL--QTSPCDQYECQNGAQCIVVQ--QEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVMLNQTLNLVVDKGAPKSL 1262 HSP 2 Score: 327.791 bits (839), Expect = 2.339e-91 Identity = 208/693 (30.01%), Postives = 331/693 (47.76%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPK------------------------AFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSA--SCNWTS-PCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRG------------------------LDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSED------EGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAH-FSHLQRLSRLDISNNKISVIKP------------------------YVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQA-EDTPQEIVAKC 758
CP +C C+ VDC L P IP A L L++NNI I AF+ LK L R+ L+ N+++++P+ F P+LT L L NQI+ I +F + ++ L L++N I CI AF L L +L+L +NNI + +FN + ++TL L N C C LAWL+DWL++ C PV L+ ++ + C S +CN S CP CSCS +VDC +G+ IP+++P+ E++L N + +P+ G F L +++S+N IS I +F+G +S+ L L N +T I L G L L L L++N + IS G F L + ++ NP CDCH+ W D+L+ ++ A C++P L ++++ ++ FRC GSED C CP +C C GT++ CS+ +L +PS++P+ ++L L+ NDI LE F L L ++++SNN+I ++ +F L+ L TL++ N I C+ F+GL +R+LSL+ N I+ I AF S++ + L SNP C+C++ WL +W++ + I G +C P L + F C E++ ++ +C
Sbjct: 34 CPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSIIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGVTGVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTIGQFTLCMAPVHLRGFSVADVQKKEYVCPGPHSEAPACNANSLSCPSACSCSNNIVDCRGKGLTEIPANLPEGIVEIRLEQNSIKSIPA-GAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIPKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFVPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRNRFSSECFMDLVCPEKCRCEGTIVDCSNQKLARIPSHLPEYTTDLRLNDNDISVLEATGIFKKLPNLRKINLSNNRIKEVREGAFDGAAGVQELMLTGNQLETMHGRMFRGLSSLKTLMLRSNLISCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHMAWLGRWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRC 725 HSP 3 Score: 62.3882 bits (150), Expect = 2.255e-8 Identity = 35/112 (31.25%), Postives = 53/112 (47.32%), Query Frame = 0
Query: 888 GSCLPSAVNTYTCSCVPGFSGPDC-SVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCE 998
G C ++ C C PG++GP C + D C H C++ GTC+ +Y C C + G C+ +S C +C HG C + + Y C C PG++G +CE
Sbjct: 1341 GLCRSVEKDSVVCECHPGWTGPLCDQEARDPCLGHSCRH-GTCMATGDSYVCKCAEGYGGALCD------QKNDSASACSAFKCHHGQCHISDRGEPY-CLCQPGFSGHHCE 1444
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Match: gi|311033528|sp|O75094.3|SLIT3_HUMAN (RecName: Full=Slit homolog 3 protein; Short=Slit-3; AltName: Full=Multiple epidermal growth factor-like domains protein 5; Short=Multiple EGF-like domains protein 5; Flags: Precursor) HSP 1 Score: 812.372 bits (2097), Expect = 0.000e+0 Identity = 425/997 (42.63%), Postives = 607/997 (60.88%), Query Frame = 0
Query: 142 CLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLS------SASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCIS------------------------PGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGR--YVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGT-CFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSI 1105
C + ++CP C C++ IVDCR K L PA++P G E+RLEQN+I +IP AF + K L+RID+S N+I I D F GL LTSLVLYGN+I I F L SLQLLLLN+NKI C+ N F L+ L+LLSLYDN +QT+ G F L S+QTLHL +N F C C L WL D+LQ NP+ETSGARC +P +L K+IS + + C S+ C CP C C +VDCS + + IPS +P+ +L+L+DN + V+ + G+F LP L +NLS N I + EG+F+GA S+ L L+ N L T+ GR+ RGL GL TL L +N I C+S PGAF L LS N+LSNP C+CH+AW G WLR + S C P L++ +Q++ + F C G+E+ C CP +C C TV+RCS+ L+ +P +PK+ +ELYL+ N + + S L+ L+ +D+SNN IS++ Y F+N++ LSTLI+SYN ++CI HAF+GL LR+L+LHGN IS +P +F+D S++HLALG+NPL CDC+L+WLS+W+K Y EPGIA+CS P+ +A+ L+LTTP F+C+ IVAKC+ C SSPC+N TC + C C Y G C I+ C NPC HGGTC + ++ + + C CP G+ G RCE D+CE N C+NNATC D + + C CP Y+GE C ID C+ + C + C+P ++C CVPG+SG C DDC +H C++G C+D Y+C C F+G +CE P + + Q+SPC Q+EC++G C + + ++ C+C PG+ G CE L +++F K +Y ++ +P ANI+++V T + +G+L+Y G N+ LA+EL++G +R+ YD + P +T++S E ++DG H ++++ + L L VD G +S+
Sbjct: 273 CNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPR-ELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGP-VDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPLD-KGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPPPMVLL--QTSPCDQYECQNGAQCIVVQ--QEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSL 1262 HSP 2 Score: 332.028 bits (850), Expect = 8.704e-93 Identity = 211/695 (30.36%), Postives = 333/695 (47.91%), Query Frame = 0
Query: 147 VNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPK------------------------AFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSS--ASCNWTS-PCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRG------------------------LDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSED------EGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAH-FSHLQRLSRLDISNNKISVIKP------------------------YVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQA-EDTPQEIVAKC 758
V CP +C C+ VDC L P IP A L L++NNI I AF+ LK L R+ L+ N+++++P+ F P+LT L L NQI+ I +F + ++ L L++N I CI AF L L +L+L +NNI + +FN + ++TL L N C C LAWL+DWL++ C PV L+ ++ + C S SCN S CP PC+CS +VDC +G+ IP+++P+ E++L N + +P+ G F L +++S+N IS I +F+G +S+ L L N +T I L G L L L L++N + IS G F L + ++ NP CDCH+ W D+L+ ++ A C++P L ++++ ++ FRC GSED C CP +C C GT++ CS+ +L +PS++P+ ++L L+ N++ LE F L L ++++SNNKI ++ VF L+ L TL++ N I C+ F+GL +R+LSL+ N I+ I AF S++ + L SNP C+C+L WL +W++ + I G +C P L + F C E++ ++ +C
Sbjct: 32 VACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPA-GAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRC 725 HSP 3 Score: 58.5362 bits (140), Expect = 4.054e-7 Identity = 32/112 (28.57%), Postives = 53/112 (47.32%), Query Frame = 0
Query: 888 GSCLPSAVNTYTCSCVPGFSGPDC-SVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCE 998
G C ++ C C PG++GP C + D C H C + G C+ +Y C C + G C+ + ++ C +C HG C + + Y C C PG++G++C+
Sbjct: 1341 GLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHH-GKCVATGTSYMCKCAEGYGGDLCD------NKNDSANACSAFKCHHGQCHISDQGEPY-CLCQPGFSGEHCQ 1444
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Match: gi|33112440|sp|O94813.1|SLIT2_HUMAN (RecName: Full=Slit homolog 2 protein; Short=Slit-2; Contains: RecName: Full=Slit homolog 2 protein N-product; Contains: RecName: Full=Slit homolog 2 protein C-product; Flags: Precursor) HSP 1 Score: 810.061 bits (2091), Expect = 0.000e+0 Identity = 418/1004 (41.63%), Postives = 602/1004 (59.96%), Query Frame = 0
Query: 145 SRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLS--------------SASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHC------------------------ISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRC-KGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGR--YVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVN 1107
S ++CP C C++ IVDCR K L P ++P TE+RLEQN I IPP AF K LRRIDLSNN+I + D F GL L SLVLYGN+I + S F L SLQLLLLN+NKI C+ +AF L L+LLSLYDN +QT+ GTF+ L ++QT+HL +N F C C L WL D+L NP+ETSGARC +P +L K+I + + C S C CP C C VDCS + + IP IP+ T EL+L++N V+ + G+F LP L +N S N I+ IEEG+FEGA ++ + L+ N L ++ ++ +GL+ L TL L +N I C ++PGAFD L LS N+L+NP C+C++AW G+WLR + + C P L++ +Q++ + F C G++D C CP EC C TV+RCS+ LK +P +P++ +ELYLD N L S+ + L+ +D+SNN+IS + F+N+T+L TLI+SYN ++CI P F GL LR+LSLHGN IS++P AF+D ++++HLA+G+NPL CDCN++WLS W+K++Y EPGIA+C+ P +A+ L+LTTP F CQ I+AKC+PC S+PC+N TC + + C C G+ G C+ I AC NPC HGGTC + E + C C G+ G CE +D+CE N C+NN+TC D + + C CP Y+GE C K+D C + C + C+ + + C C PG+ G C + DDC+ + C+NG C D Y+C C ++G +CE P + + P++SPC +C++G + + ++ +C+C PGY G+ CE L S++F K +Y Q+ +P NIT+++ T E SG+L+Y G +H+AVEL++GR+R SYD G++P S ++S E I+DG H +++LA ++L+L VD G + I N
Sbjct: 269 SVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPPGAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPTECTCLDTVVRCSNKGLKVLPKGIPRDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGP-VDVNILAKCNPCLSNPCKNDGTCNSDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGEEDGFWCICADGFEGENCEVNVDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTP-KGFKCDCTPGYVGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCICPEGYSGLFCEFSPPM--VLPRTSPCDNFDCQNGAQCIVRINEP-ICQCLPGYQGEKCEKLVSVNFINKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLALDQSLSLSVDGGNPKIITN 1266 HSP 2 Score: 319.701 bits (818), Expect = 1.170e-88 Identity = 204/687 (29.69%), Postives = 317/687 (46.14%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIV------------------------SIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNC---LDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRG------------------------LDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCK--------GSED------EGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAH-FSHLQRLSRLDISNNKISVIKP------------------------YVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKC 745
CP +C C+ VDC L P +IP L L NNI +I AF+ LK L R+ L+ N ++L P+ F G +L L L NQI+ I +F ++ L L+ N+I CI AF L L +L+L +NNI L +FN + L+T L N C C LAWL+DWL++ P +C P L+ ++ + C + SC+ CP C+CS +VDC +G+ IP+++P+T E++L N + V+P G F+ L ++LS N IS + +F+G RS++ L L N +T + L G L L L L++N + I+ G F L + ++ NP CDCH+ W D+L + ++ A C +P L ++++ ++ FRC G+ED C CP +C C GT + CS+ +L +P ++P+ +EL L+ N+ LE F L +L +++ SNNKI+ I+ +F L L TL++ N I C+ +F GL +R+LSL+ N I+ + AFD +S++ L L +NP C+C L WL +W++ + I G +C P L + F C
Sbjct: 28 CPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKTDFAGLRHLRVLQLMENKISTIERGAFQDLKELERLRLNRNHLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLH-CPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNTIKVIPP-GAFSPYKKLRRIDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTIAKGTFSPLRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQKLNKIPEHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRITCVGNDSFIGLSSVRLLSLYDNQITTVAPGAFDTLHSLSTLNLLANPFNCNCYLAWLGEWLRKKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTC 712 HSP 3 Score: 69.707 bits (169), Expect = 1.336e-10 Identity = 40/125 (32.00%), Postives = 63/125 (50.40%), Query Frame = 0
Query: 754 IVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNI-DACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEID---ECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKI 874
I+ C+PCH C + TC+ F C+C G+ G +C++ D C GN C HG TC Y C C G+ G+ C+ E D C++ C++ + + C+C GY+G++C +I
Sbjct: 1329 ILPGCEPCHKKVCAH-GTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQAIKCKHGKCRLSGLGQPYCECSSGYTGDSCDREI 1451 HSP 4 Score: 65.0846 bits (157), Expect = 3.517e-9 Identity = 45/130 (34.62%), Postives = 64/130 (49.23%), Query Frame = 0
Query: 888 GSCLPSAVNTYTCSCVPGFSGPDCSVSLDD-CRSHMCQNGGTCLDGNG-TYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQME 1015
G+C PS+ +TC C G+ GP C +D C + C + GTCL N +YSC C G C+ E + + PCQ +CKHG C + + Y C+C GYTG C+ S ++ Y Q +
Sbjct: 1344 GTCQPSSQAGFTCECQEGWMGPLCDQRTNDPCLGNKCVH-GTCLPINAFSYSCKCLEGHGGVLCDEEEDLFN------PCQAIKCKHGKCRLSGLGQPY-CECSSGYTGDSCDREISCRGERIRDYYQKQ 1465
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Match: gi|145559530|sp|O75093.4|SLIT1_HUMAN (RecName: Full=Slit homolog 1 protein; Short=Slit-1; AltName: Full=Multiple epidermal growth factor-like domains protein 4; Short=Multiple EGF-like domains protein 4; Flags: Precursor) HSP 1 Score: 809.29 bits (2089), Expect = 0.000e+0 Identity = 438/1007 (43.50%), Postives = 598/1007 (59.38%), Query Frame = 0
Query: 142 CLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLS------------SASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCI------------------------SPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRC-KGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVP--ENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEG 1109
C S +CP C C++GIVDCR K L PA++P TE+RLE N I SIPP AF + LRRIDLSNN+I I D F GL L SLVLYGN+I + F L +LQLLLLN+NKI CI P+AF L+ L LLSLYDN IQ+L GTF L ++QTLHL +N F C C+L WL D+L+ NP+ETSGARC +P +L K+I + + C ++ CN CP C C VV+CS + IP IP++T EL+L++N + ++ + G+F L L +NLS N +S IE+G+FEGA S+ L L+ N L +IR + RGLDGL TL L NN I CI SPGAFD L LS N+L+NP C+C +AW G WLR + + C P LR LQ++ P FRC +G E+ GCL + CP+EC C TV+RCS+ L+ +P +PK +ELYLD N L S + L +D+SNNKIS + F N+++L+TLI+SYN ++CI P AF GL LR+LSLHGN IS + F D S++HLA+G+NPL CDC+L+WLS W+K Y EPGIA+C+ P+ + L+LTTP F+CQ T + AKCD C SSPCQN+ TC + C C GY G CE ++D+C PC +GGTC E+ + C CP G+ G C D+C + C N C D V + C CP Y G+ C +D C + C + C+ + + C C+PG++G +CS + DDCR H CQNG C+D +YSC C ++G+ CE+ P H+ SPC+ EC++G + + ++ VC+C PG+ G CE L S++F + Y Q L P ANIT++V T E +G+L+Y G N+H+AVEL++G +RVSYD G+YP S ++S E I+DG H ++++A + + L +D G ++ N G
Sbjct: 275 CTLSSGSCPAMCTCSNGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETSGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYQLNSECNSDVVCPHKCRCEANVVECSSLKLTKIPERIPQSTAELRLNNNEISILEATGMFKKLTHLKKINLSNNKVSEIEDGAFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNQITTVSPGAFDTLQSLSTLNLLANPFNCNCQLAWLGGWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCEEGQEEGGCLPRPQCPQECACLDTVVRCSNKHLRALPKGIPKNVTELYLDGNQF-TLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDISTLQEGIFADVTSLSHLAIGANPLYCDCHLRWLSSWVKTGYKEPGIARCAGPQDMEGKLLLTTPAKKFECQGPPT-LAVQAKCDLCLSSPCQNQGTCHNDPLEVYRCACPSGYKGRDCEVSLDSCSSGPCENGGTCHAQEGEDAPFTCSCPTGFEGPTCGVNTDDCVDHACANGGVCVDGVGNYTCQCPLQYEGKACEQLVDLCSPDLNPCQHEAQCVGTP-DGPRCECMPGYAGDNCSENQDDCRDHRCQNGAQCMDEVNSYSCLCAEGYSGQLCEIPP---HLPAPKSPCEGTECQNGANCVDQGNRP-VCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVAFDQMVNLSIDGGSPMTMDNFG 1274 HSP 2 Score: 317.775 bits (813), Expect = 5.188e-88 Identity = 204/688 (29.65%), Postives = 318/688 (46.22%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLR------------------------RIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSA----SCNWTS-PCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTT------------------------IRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCK--------GSED----EGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLE-MAHFSHLQRLSRLDISNNKIS------------------------VIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQ 746
CP C C VDC L+ P +IP L L NNI I F LK LR R+ L+ N++ ++P+ F L+ L L N I+ I +F L+ L L+ N+I CI AF L L +L+L +NNI T+ +FN + L+T L N C C LAWL+ WL++ P +C P L+ ++ + +C A +C +S CP C+CS G+VDC +G+ IP+++P+T E++L N + +P G F+ L ++LS N I+ I +F+G RS++ L L N +T IR + L L+ L L++N I ++ G F L + ++ NP CDC++ W D+LR+ ++ A CA+P L ++++ ++ FRC G+ED C CP +C C V+ CS +L +P +P+ +EL L+ N+I LE F L L ++++SNNK+S I+ +F L L TL++ N I CI +F+GL ++R+LSL+ N I+ + AFD S++ L L +NP C+C L WL W++ + I G +C P L + F+C+
Sbjct: 34 CPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLHMLPELLFQNNQALSRLDLSENAIQAIPRKAFRGATDLKNLQLDKNQISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAGRVPTCTLSSGSCPAMCTCSNGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPP-GAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETSGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYQLNSECNSDVVCPHKCRCEANVVECSSLKLTKIPERIPQSTAELRLNNNEISILEATGMFKKLTHLKKINLSNNKVSEIEDGAFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNQITTVSPGAFDTLQSLSTLNLLANPFNCNCQLAWLGGWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCE 720 HSP 3 Score: 236.498 bits (602), Expect = 6.403e-62 Identity = 143/461 (31.02%), Postives = 216/461 (46.85%), Query Frame = 0
Query: 365 TSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDN-----------------VLDVVPSV------GLFN----------------LLP--------ALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEG-----CLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLII------------------------SYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAED 749
S CP C+C+ VDC G++ IP +IP+ T+ L+L+ N VL ++ + G F+ +LP ALS L+LS N I I +F GA + L L N ++ I R L GL L L+NNNI I +F+ +PKL + SN + CDCH+AW WLR + C+ PA LR + E+ F C G + G L CP C C+ ++ C L +P+N+P+ +E+ L++N I + FS ++L R+D+SNN+I+ I P F L L++L++ + N+I CI+P AF L +L +LSL+ N I + F +I L L NP +CDCNLKWL+ +++ IE A+C+ P+ LAN + F+C A++
Sbjct: 31 ASACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLHMLPELLFQNNQALSRLDLSENAIQAIPRKAFRGATDLKNLQLDKNQISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAGRVPTCTLSSGSCPAMCTCSNGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETSGARCASPRRLANKRIGQIKSKKFRCSAKE 491
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Match: gi|45477219|sp|O88279.1|SLIT1_RAT (RecName: Full=Slit homolog 1 protein; Short=Slit-1; AltName: Full=Multiple epidermal growth factor-like domains protein 4; Short=Multiple EGF-like domains protein 4; Flags: Precursor) HSP 1 Score: 806.209 bits (2081), Expect = 0.000e+0 Identity = 435/1007 (43.20%), Postives = 598/1007 (59.38%), Query Frame = 0
Query: 142 CLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLS------------SASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHC------------------------ISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRC-KGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVP--ENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEG 1109
C S +CP C C++GIVDCR K L PA++P TE+RLE N I SIPP AF + LRRIDLSNN+I I D F GL L SLVLYGN+I + F L +LQLLLLN+NKI CI P+AF L+ L LLSLYDN IQ+L GTF L ++QTLHL +N F C C+L WL D+L+ NP+ET+GARC +P +L K+I + + C ++ C CP C C VV+CS + IP IP++T EL+L++N + ++ + GLF L L +NLS N +S IE+G+FEGA S+ L L+ N L ++R + RGLDGL TL L NN I C ISPGAFD L LS N+L+NP C+C +AW GDWLR + + C P LR LQ++ P FRC +G E+ GCL + CP+EC C TV+RCS+ L+ +P +PK +ELYLD N L S + L +D+SNNKIS + F N+++L+TLI+SYN ++CI P AF GL LR+LSLHGN +S + F D S++HLA+G+NPL CDC+L+WLS W+K Y EPGIA+C+ P + L+LTTP F+CQ + + AKCDPC SSPCQN+ TC + C C GY G CE ++D+C NPC +GGTC E+ + C CP G+ GL C D+C + C N C D + + C CP Y+G C +D C + C + C+ + C CVPG++G +CS + DDC+ H CQNG C+D +Y+C C ++G+ CE+ P P++S C+ EC++G + + S+ VC+C PG+ G CE L S++F + Y Q L P ANIT++V T E +G+L+Y G N+H+AVEL++G +RVSYD G+YP S ++S E I+DG H ++++ + + L +D G ++ N G
Sbjct: 275 CTLSSGSCPAMCSCSNGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSGLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGATSVSELHLTANQLESVRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCQLAWLGDWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDGNQF-TLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDVSTLQEGIFADVTSLSHLAIGANPLYCDCHLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAKKFECQGPPS-LAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRNCEVSLDSCSSNPCGNGGTCHAQEGEDAGFTCSCPSGFEGLTCGMNTDDCVKHDCVNGGVCVDGIGNYTCQCPLQYTGRACEQLVDFCSPDLNPCQHEAQCVGTPEGPR-CECVPGYTGDNCSKNQDDCKDHQCQNGAQCVDEINSYACLCAEGYSGQLCEIPPA-----PRNS-CEGTECQNGANCVDQGSRP-VCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSIDGGSPMTMDNFG 1271 HSP 2 Score: 317.775 bits (813), Expect = 5.078e-88 Identity = 203/688 (29.51%), Postives = 319/688 (46.37%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLR------------------------RIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSA----SCNWTS-PCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTT------------------------IRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCK--------GSED----EGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAH-FSHLQRLSRLDISNNKISVIKPYVFANLTRLS------------------------TLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQ 746
CP C C VDC L+ P +IP L L NNI I F LK LR R+ L+ N+++++P+ F L+ L L N ++ + +F L+ L L+ N+I CI AF L L +L+L +NNI T+ +FN + L+T L N C C LAWL+ WL++ P +C P L+ ++ + +C A +C +S CP CSCS G+VDC +G+ IP+++P+T E++L N + +P G F+ L ++LS N I+ I +F+G RS++ L L N +T IR + L L+ L L++N I ++ G F L + ++ NP CDC++ W D+LR+ + A CA+P L ++++ ++ FRC G+ED C CP +C C +V+ CS +L +P +P+ +EL L+ N+I LE F L L ++++SNNK+S I+ F T +S TL++ N I CI +F+GL ++R+LSL+ N I+ I AFD +++ L L +NP C+C L WL W++ + I G +C P L + F+C+
Sbjct: 34 CPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENSLQAVPRKAFRGATDLKNLQLDKNQISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAAQVPACTLSSGSCPAMCSCSNGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPP-GAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSGLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGATSVSELHLTANQLESVRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCQLAWLGDWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCE 720 HSP 3 Score: 114.005 bits (284), Expect = 4.621e-24 Identity = 69/241 (28.63%), Postives = 101/241 (41.91%), Query Frame = 0
Query: 564 CPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHL------------------------------------------------QRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVA 756
CP C CTGT + C L+ +P N+P+ L L+ N+I + F+ L Q LSRLD+S N + + F T L L + N+I CI+ AF L L +L+L+ N+I+ IP S+F+ + L SN L CDC+L WLSQW++ + +CS P SL V +S F C + ++ A
Sbjct: 34 CPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENSLQAVPRKAFRGATDLKNLQLDKNQISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAAQVPA 274
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Match: gi|341942040|sp|Q9R1B9.2|SLIT2_MOUSE (RecName: Full=Slit homolog 2 protein; Short=Slit-2; Contains: RecName: Full=Slit homolog 2 protein N-product; Contains: RecName: Full=Slit homolog 2 protein C-product; Flags: Precursor) HSP 1 Score: 806.209 bits (2081), Expect = 0.000e+0 Identity = 421/997 (42.23%), Postives = 600/997 (60.18%), Query Frame = 0
Query: 145 SRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLS------SASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHC------------------------ISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRC-KGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVP--ENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGT-CFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVN 1107
S ++CP C C++ IVDCR K L P ++P TE+RLEQN+I IPP AF K LRR+DLSNN+I + D F GL L SLVLYGN+I + S F L SLQLLLLN+NKI C+ +AF L L+LLSLYDN +QT+ GTF+ L ++QT+HL +N F C C L WL D+L NP+ETSGARC +P +L K+I + + C S C CP C C VDCS + + IP IP+ T EL+L++N V+ + G+F LP L +N S N I+ IEEG+FEGA ++ + L+ N L ++ ++ +GL+ L TL L +N I C ++PGAFD L LS N+L+NP C+CH+AW G+WLR + + C P L++ +Q++ + F C G++D C CP EC C TV+RCS+ LK +P +PK+ +ELYLD N L S+ + L+ +D+SNN+IS + F+N+T+L TLI+SYN ++CI P F GL LR+LSLHGN IS++P AF+D ++++HLA+G+NPL CDCN++WLS W+K++Y EPGIA+C+ P +A+ L+LTTP F CQ I AKC+PC S+PC+N TC + C C G+ G C+ I AC NPC HGGTC + EN + C C G+ G CE ID+CE N C+NN+TC D + + C CP Y+GE C K+D C + C + C+ + + C C PG+ G C + DDC+ + C+NG C D Y+C C ++G +CE P + + P++SPC +C++G C + + + +C+C PGY G+ CE L S++F K +Y Q+ +P NIT+++ T E SG+L+Y G +H+AVEL++GR+R SYD G++P S ++S E I+DG H +++L +L+L VD G + I N
Sbjct: 269 SVLHCPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVLPKGIPKDVTELYLDGNQF-TLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTLILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCDCNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGP-VDITIQAKCNPCLSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENAGFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLDFCAQDLNPCQHDSKCILTP-KGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVNGYTCVCPEGYSGLFCEFSPPM--VLPRTSPCDNFDCQNGAQCIIRIN--EPICQCLPGYLGEKCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYRGRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITN 1258 HSP 2 Score: 327.791 bits (839), Expect = 1.872e-91 Identity = 204/679 (30.04%), Postives = 318/679 (46.83%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIV------------------------SIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNC---LDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRG------------------------LDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSED------EGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAH-FSHLQRLSRLDISNNKISVIKP------------------------YVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKC 745
CP +C C+ VDC L P +IP L L NNI +I AF+ LK L R+ L+ N ++L P+ F G +L L L NQI+ I +F ++ L L+ N+I CI AF L L +L+L +NNI L +FN + L+T L N C C LAWL+DWL++ P +C P L+ ++ + C + SC+ CP C+CS +VDC +G+ IP+++P+T E++L N + V+P G F+ L L+LS N IS + +F+G RS++ L L N +T + L G L L L L++N + ++ G F L + ++ NP CDCH+ W D+L + ++ A C +P L ++++ ++ FRC G+ED C CP +C C GT + CS+ +L +P ++P+ +EL L+ N+ LE F L +L +++ SNNKI+ I+ +F L L TL++ N I C+ +F GL +R+LSL+ N I+ + AFD +S++ L L +NP C+C+L WL +W++ + I G +C P L + F C
Sbjct: 28 CPAQCSCSGSTVDCHGLALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDLKELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDYNQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLAWLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLH-CPAACTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPP-GAFSPYKKLRRLDLSNNQISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAFQDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPIETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTTVDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIEEGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSVRLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPRCQKPYFLKEIPIQDVAIQDFTC 704 HSP 3 Score: 65.4698 bits (158), Expect = 2.797e-9 Identity = 39/125 (31.20%), Postives = 61/125 (48.80%), Query Frame = 0
Query: 754 IVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNI-DACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEID---ECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKI 874
I+ C+PCH C + C+ F C+C G+ G +C++ D C GN C HG TC Y C C G+ G+ C+ E D C+ C++ V + C+C G++G++C +I
Sbjct: 1321 ILPGCEPCHKKVCAH-GMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHG-TCLPINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTGDSCDREI 1443 HSP 4 Score: 64.3142 bits (155), Expect = 5.834e-9 Identity = 44/130 (33.85%), Postives = 65/130 (50.00%), Query Frame = 0
Query: 888 GSCLPSAVNTYTCSCVPGFSGPDCSVSLDD-CRSHMCQNGGTCLDGNG-TYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQME 1015
G C PS+ + +TC C G+ GP C +D C + C + GTCL N +YSC C G C+ E + + PCQ +CKHG C + + Y C+C+ G+TG C+ S ++ Y Q +
Sbjct: 1336 GMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVH-GTCLPINAFSYSCKCLEGHGGVLCDEEEDLFN------PCQMIKCKHGKCRLSGVGQPY-CECNSGFTGDSCDREISCRGERIRDYYQKQ 1457
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Match: gi|45477282|sp|Q80TR4.2|SLIT1_MOUSE (RecName: Full=Slit homolog 1 protein; Short=Slit-1; Flags: Precursor) HSP 1 Score: 805.823 bits (2080), Expect = 0.000e+0 Identity = 437/1013 (43.14%), Postives = 598/1013 (59.03%), Query Frame = 0
Query: 136 TAGESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLS------------SASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHC------------------------ISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRC-KGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVP--ENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEG 1109
AG C S +CP C C+ GIVDCR K L PA++P TE+RLE N I SIPP AF + LRRIDLSNN+I I D F GL L SLVLYGN+I + F L +LQLLLLN+NKI CI P+AF L+ L LLSLYDN IQ+L GTF L ++QTLHL +N F C C+L WL D+L+ NP+ET+GARC +P +L K+I + + C ++ C CP C C VV+CS + IP IP++T EL+L++N + ++ + GLF L L +NLS N +S IE+G+FEGA S+ L L+ N L +IR + RGLDGL TL L NN I C ISPGAFD L LS N+L+NP C+CH++W GDWLR + + C P LR LQ++ P FRC +G E+ GCL + CP+EC C TV+RCS+ L+ +P +PK +ELYLD N L S + L +D+SNNKIS + F N+++L+TLI+SYN ++CI P AF GL LR+LSLHGN +S + F D S++HLA+G+NPL CDC L+WLS W+K Y EPGIA+C+ P + L+LTTP F+CQ + + AKCDPC SSPCQN+ TC + C C GY G CE ++D C NPC +GGTC E+ + C CP G+ G C + D+C + C N C D V + C CP Y+G C +D C + C + C+ + + C C+ G++G +CS + DDC+ H CQNG C+D +Y+C C ++G+ CE+ P P+SS C+ EC++G + + S+ VC+C PG+ G CE L S++F + Y Q L P ANIT++V T E +G+L+Y G N+H+AVEL++G +RVSYD G+YP S ++S E I+DG H ++++ + + L +D G ++ N G
Sbjct: 269 AAGAPACTLSSGSCPAMCSCSSGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSSLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCHLSWLGDWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCEEGQEEVGCLPRPQCPQECACLDTVVRCSNKHLQALPKGIPKNVTELYLDGNQF-TLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLHGNDVSTLQEGIFADVTSLSHLAIGANPLYCDCRLRWLSSWVKTGYKEPGIARCAGPPEMEGKLLLTTPAKKFECQGPPS-LAVQAKCDPCLSSPCQNQGTCHNDPLEVYRCTCPSGYKGRHCEVSLDGCSSNPCGNGGTCHAQEGEDAGFTCSCPSGFEGPTCGVDTDDCVKHACVNGGVCVDGVGNYTCQCPLQYTGRACEQLVDFCSPDMNPCQHEAQCVGTP-DGPRCECMLGYTGDNCSENQDDCKDHKCQNGAQCVDEVNSYACLCVEGYSGQLCEIPPA-----PRSS-CEGTECQNGANCVDQGSRP-VCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANITLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVTFDQMVNLSIDGGSPMTMDNFG 1271 HSP 2 Score: 320.087 bits (819), Expect = 8.323e-89 Identity = 204/688 (29.65%), Postives = 321/688 (46.66%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLR------------------------RIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSA----SCNWTS-PCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTT------------------------IRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCK--------GSED----EGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAH-FSHLQRLSRLDISNNKIS------------------------VIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQ 746
CP C C VDC L+ P +IP L L NNI I F LK LR R+ L+ N+++++P+ F L+ L L N ++ + +F L+ L L+ N+I CI AF L L +L+L +NNI T+ +FN + L+T L N C C LAWL+ WL++ P +C P L+ ++ + G +C A +C +S CP CSCS G+VDC +G+ IP+++P+T E++L N + +P G F+ L ++LS N I+ I +F+G RS++ L L N +T IR + L L+ L L++N I ++ G F L + ++ NP CDC++ W D+LR+ + A CA+P L ++++ ++ FRC G+ED C CP +C C +V+ CS +L +P +P+ +EL L+ N+I LE F L L ++++SNNK+S I+ +F L L TL++ N I CI +F+GL ++R+LSL+ N I+ I AFD +++ L L +NP C+C+L WL W++ + I G +C P L + F+C+
Sbjct: 34 CPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENFLQAVPRKAFRGATDLKNLQLDKNRISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKGEFSCSGQGEAAGAPACTLSSGSCPAMCSCSSGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPP-GAFSPYRKLRRIDLSNNQIAEIAPDAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSLAKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETTGARCASPRRLANKRIGQIKSKKFRCSAKEQYFIPGTEDYHLNSECTSDVACPHKCRCEASVVECSSLKLSKIPERIPQSTTELRLNNNEISILEATGLFKKLSHLKKINLSNNKVSEIEDGTFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHNDSFTGLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPFNCNCHLSWLGDWLRKRKIVTGNPRCQNPDFLRQIPLQDVAFPDFRCE 720 HSP 3 Score: 109.383 bits (272), Expect = 1.178e-22 Identity = 67/233 (28.76%), Postives = 96/233 (41.20%), Query Frame = 0
Query: 564 CPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHL------------------------------------------------QRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAE 748
CP C CTGT + C L+ +P N+P+ L L+ N+I + F+ L Q LSRLD+S N + + F T L L + N I CI+ AF L L +L+L+ N+I+ IP S+F+ + L SN L CDC+L WLSQW++ + +CS P SL V + F C +
Sbjct: 34 CPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNGNNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENFLQAVPRKAFRGATDLKNLQLDKNRISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKGEFSCSGQ 266
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Match: gi|449061890|sp|G5EFX6.1|SLIT1_CAEEL (RecName: Full=Slit homolog 1 protein; Short=Slt-1; Flags: Precursor) HSP 1 Score: 752.666 bits (1942), Expect = 0.000e+0 Identity = 397/969 (40.97%), Postives = 571/969 (58.93%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDW-LQKNPVETSGARCETPVKLQRKKISSLHLGRSNC------LDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLE-QHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEG 1109
CP C C VDCRD L P ++P TE+RLEQN I SIP +FK LK L R+DLS N I I F GL L +LVLYGN I + +F L SLQLLLLN+N++ CI F+ + L +LSLYDN+I+++ TF L+SL TLHL KN C C+L WL LQKN +ETSGARCE P +L++KK ++L + C + + + SC S CP C C VDC++RG+ IP+ IP+ +L LS N + V ++L L L+LS N I+ I + SFE + L L+ N L +L L L L NNI C S F+ ++ ++ N + CDC + WLRS ++ S D + + RC +E C + + CP +C C V+RCS+ L + PS +P + +ELYLD N I+++ + L L++LD+S+N++ ++ F+NLTRLSTLIISYN+++C+QP AF+GL+ LRILSLHGN IS +P SAF + SITH+A+GSN L CDCN+ W S+W+K+++IE GIA+C YP +++N L+LT F C ++ P ++ KCD C +SPC+N A C ++ C+C PG+YG CE IDACYG+PC + TC V + GR+ C C G+ G CE ID+C ++ C+N C D + +RCDCP Y G+ C K++ C + C N G C+P +Y+C C PGF+G +C ++DDC++ CQNGG+C+DG +Y C C P +AG+YCE+ P++ Y ++ CQQ C G C ++S D+ CKC G++G C+ S+ F GAY ++PL + ITM + T G+L+Y G + ++ EL+ GR+++ Y +GN+P S M+S K++DGLPHRI I + L++D + + N G
Sbjct: 260 CPLPCTCTGTTVDCRDSGLTYVPTNLPPSTTEIRLEQNQISSIPSHSFKNLKNLTRLDLSKNIITEIQPKAFLGLHNLHTLVLYGNNITDLKSDTFEGLGSLQLLLLNANQLTCIRRGTFDHVPKLSMLSLYDNDIKSISEVTFQNLTSLSTLHLAKNPLICDCNLQWLAQINLQKN-IETSGARCEQPKRLRKKKFATLPPNKFKCKGSESFVSMYADSCFIDSICPTQCDCYGTTVDCNKRGLNTIPTSIPRFATQLLLSGNNISTVDLNSNIHVLENLEVLDLSNNHITFINDKSFEKLSKLRELRLNDNKLHHFSSMVLDEQSNLEILDLSGNNIQCFSSIFFNKATRIREIKVIGNDLLCDCRILPLMSWLRSNSSHSIDIPPCQQFQYSDNESDKQ-----RCAAFPEETCSDDSNLCPPKCSCLDRVVRCSNKNLTSFPSRIPFDTTELYLDANYINEIPAHDLNRLYSLTKLDLSHNRLISLENNTFSNLTRLSTLIISYNKLRCLQPLAFNGLNALRILSLHGNDISFLPQSAFSNLTSITHIAVGSNSLYCDCNMAWFSKWIKSKFIEAGIARCEYPNTVSNQLLLTAQPYQFTCDSK-VPTKLATKCDLCLNSPCKNNAICETTSSRKYTCNCTPGFYGVHCENQIDACYGSPCLNNATCKVAQAGRFNCYCNKGFEGDYCEKNIDDCVNSKCENGGKCVDLINSYRCDCPMEYEGKHCEDKLEYCTKKLNPCENNGKCIP-INGSYSCMCSPGFTGNNCETNIDDCKNVECQNGGSCVDGILSYDCLCRPGYAGQYCEIPPMMDMEYQKTDACQQSACGQGECVASQNSSDFTCKCHEGFSGPSCDRQMSVGFKNPGAYLALDPLASD--GTITMTLRTTSKIGILLYYGDDHFVSAELYDGRVKLVYYIGNFPASHMYSSVKVNDGLPHRISIRTSERKCFLQIDKNPVQIVENSG 1218 HSP 2 Score: 275.018 bits (702), Expect = 2.936e-74 Identity = 182/624 (29.17%), Postives = 290/624 (46.47%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPP------------------------KAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLD----LSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGR--------------------LLRG----LDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSED------EGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSH-LQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMK 713
CP EC C D V C ++L P +IP+ L L+ N I I P ++F L +L+++ LS N IR +PD+ F +LT L L N I +S + L++L L+ N I C+ N + L +L+L N + T + + + + L L N + C C L W+ WL+K + C TP+ LQ I L C +C CP PC+C+ VDC G+ +P+++P +T E++L N + +PS N L L+ L+LS+N I+ I+ +F G ++H L L N++T ++ + RG + L+ L L++N+I IS F L LS ++ NP+ CDC++ W +N ++ A C P LR ++ LP F+CKGSE + C CP +C C GT + C+ L T+P+++P+ A++L L N+I +++ H L+ L LD+SNN I+ I F L++L L ++ N++ +L IL L GN+I + F+ I + + N LLCDC + L W++
Sbjct: 17 CPAECVCVDRTVSCVGQQLTEVPQNIPNDTIRLDLQDNEITKIGPNDFSSLMNLKALQLMDNQIVTIHNQSFSSLVFLQKLRLSRNRIRHLPDNVFQNNLKLTHLDLSENDITVVSDAQLQGPEFLEVLNLDKNHIFCLENNVISSWVSLEVLTLNGNRLTTFEEPSN---ARFRQLDLFNNPWNCDCRLRWMRKWLEKAEGQNKTV-CATPLNLQGSSIEILQDKFMTCSGNRKRRYKKTCETAEICPLPCTCTGTTVDCRDSGLTYVPTNLPPSTTEIRLEQNQISSIPSHSFKN-LKNLTRLDLSKNIITEIQPKAFLGLHNLHTLVLYGNNITDLKSDTFEGLGSLQLLLLNANQLTCIRRGTFDHVPKLSMLSLYDNDIKSISEVTFQNLTSLSTLHLAKNPLICDCNLQWLAQINLQKNIETSGARCEQPKRLRKKKFATLPPNKFKCKGSESFVSMYADSCFIDSICPTQCDCYGTTVDCNKRGLNTIPTSIPRFATQLLLSGNNISTVDLNSNIHVLENLEVLDLSNNHITFINDKSFEKLSKLRELRLNDNKLHHFSSMVLDEQSNLEILDLSGNNIQCFSSIFFNKATRIREIKVIGNDLLCDCRILPLMSWLR 635 HSP 3 Score: 188.734 bits (478), Expect = 4.619e-47 Identity = 134/461 (29.07%), Postives = 197/461 (42.73%), Query Frame = 0
Query: 365 TSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDN-VLDVVPS--VGLFNL--------------------LPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFT---------------------NILSNPIQCDCHMAWFGDWLR---SQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSED----EGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEI------------------------KCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDT 750
++ CP C C + V C + + +P +IP T L L DN + + P+ L NL L L L LSRN I + + F+ + LDLS N +T + L+G + L L+L N+I C+ L ++ +NP CDC + W WL QN T CATP +L+ ++ L C G+ + C CP C CTGT + C + L VP+N+P +E+ L+ N I + F +L+ L+RLD+S N I+ I+P F L L TL++ N I CI+ F + L +LSL+ N I I F + S++ L L NPL+CDCNL+WL+Q + IE A+C PK L T P + FKC+ ++
Sbjct: 14 SATCPAECVCVDRTVSCVGQQLTEVPQNIPNDTIRLDLQDNEITKIGPNDFSSLMNLKALQLMDNQIVTIHNQSFSSLVFLQKLRLSRNRIRHLPDNVFQNNLKLTHLDLSENDITVVSDAQLQGPEFLEVLNLDKNHIFCLENNVISSWVSLEVLTLNGNRLTTFEEPSNARFRQLDLFNNPWNCDCRLRWMRKWLEKAEGQNKT----VCATPLNLQGSSIEILQDKFMTCSGNRKRRYKKTCETAEICPLPCTCTGTTVDCRDSGLTYVPTNLPPSTTEIRLEQNQISSIPSHSFKNLKNLTRLDLSKNIITEIQPKAFLGLHNLHTLVLYGNNITDLKSDTFEGLGSLQLLLLNANQLTCIRRGTFDHVPKLSMLSLYDNDIKSISEVTFQNLTSLSTLHLAKNPLICDCNLQWLAQINLQKNIETSGARCEQPKRLRKKKFATLPPNKFKCKGSES 470 HSP 4 Score: 135.961 bits (341), Expect = 7.523e-31 Identity = 110/381 (28.87%), Postives = 160/381 (41.99%), Query Frame = 0
Query: 40 CPTECKCSDGLVDCSRANLDTFPRDIP---------------YDSHS----------IDLSFNSITQFPDVS-EWL----------------------EYAQIRHIDVSHNFIECFDSNLVNHKVEHVLELKHGRG-----CR------------------------------SDEDLLTA-GESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETP 336
CPT+C C VDC++ L+T P IP D +S +DLS N IT D S E L E + + +D+S N I+CF S N K + E+K CR SD+ A E C D CP +C C D +V C +K L FP+ IP TEL L+ N I IP +L L ++DLS+N + + ++TF+ L RL++L+ ++ NK+RC+ P AFN L L +LSL+ N+I L F+ L+S+ + +G N+ C C++AW + W++ +E ARCE P
Sbjct: 485 CPTQCDCYGTTVDCNKRGLNTIPTSIPRFATQLLLSGNNISTVDLNSNIHVLENLEVLDLSNNHITFINDKSFEKLSKLRELRLNDNKLHHFSSMVLDEQSNLEILDLSGNNIQCFSSIFFN-KATRIREIKVIGNDLLCDCRILPLMSWLRSNSSHSIDIPPCQQFQYSDNESDKQRCAAFPEETCSDDSNLCPPKCSCLDRVVRCSNKNLTSFPSRIPFDTTELYLDANYINEIPAHDLNRLYSLTKLDLSHNRLISLENNTFSNLTRLSTLI------------------------ISYNKLRCLQPLAFNGLNALRILSLHGNDISFLPQSAFSNLTSITHIAVGSNSLYCDCNMAWFSKWIKSKFIEAGIARCEYP 840
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Match: gi|767170206|dbj|BAQ58829.1| (slit protein [Daphnia magna]) HSP 1 Score: 1064.68 bits (2752), Expect = 0.000e+0 Identity = 514/970 (52.99%), Postives = 673/970 (69.38%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLD-------LSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLR--SQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEG 1109
CP C C DG+VDCRDK L R P +P ATELRLEQN I IP KAF K L+R+DLSNN+I I D F GL LTSL+LYGN+I + F+ L SLQLLLLN+N+I C+ ++F L L+LLSLYDNNI T+ NGTF+ L ++QTLHLG+N F C C L WL D+L + PVETSG RCE+P +LQR++I+ L +S C D L + C CP CSC +VDCS +G+K IP ++P T EL L+DN + + + G+F LP L ++L RN IS IE G+FEGA ++ L+L+ N + I ++ GL L TL L N I C++PG+FD L L+ NILSNP+ C+CHMAWFGDWLR Q ++ CA+PA ++D LQ++P FRC +D+GCL + YCP +C CTGT++RCS A+L+ +P N+P E +ELYLDVN+I ++ SHL+ L+RLD+SNN +SV+ FANLT+LS+LI+SYN+++C+Q +AFSGL LRILSLHGN +SMIP AF D SITHLALGSNP CDCNL+WL++W+K Y+EPGIA+C+ P L + L+LT+P F+C+ + E++AKCD C++ PC+N ATCR ++ C C PGY+G +CE IDACYGNPC + C V E GR+ C C GY G RCE I++C + C NN TC D+V RCDC GY+G C KI C + C NG +CL + YTC C GFSG +C+++ DDC +HMCQNGGTC+DG Y+C C PEF+GK+CE P++S +YPQ+SPCQQH+C++G CF P+ DY+CKC GYTGK CE+LTS+SF Q G+Y +M L TKP ANITM T E +G+L+Y G ++H+AVELF+GRIR SYDVGNYPVS MFSYE +SDG H +++L+Q KN TLRVD G +RS++NEG
Sbjct: 320 CPLMCRCADGVVDCRDKSLSRIPPYLPDSATELRLEQNQITEIPAKAFAAYKKLKRVDLSNNQISSIAGDAFHGLKSLTSLILYGNRITELPAGMFHGLTSLQLLLLNANRISCVRRDSFKDLHNLNLLSLYDNNIPTMLNGTFDSLRNIQTLHLGRNPFLCDCYLTWLADYLHRKPVETSGVRCESPRRLQRRRIAVLRDEKSRCSDRDLERNLLEISPCEDDPSCPAECSCKGTIVDCSNKGLKEIPRELPLYTTELILNDNQIVRIKADGMFRDLPNLLKVDLRRNQISAIENGAFEGANTLLDLNLAENIIQEIHNKMFTGLVSLRTLSLFQNKITCVTPGSFDYLRSLTSLNILSNPLHCNCHMAWFGDWLRKKEQELSTGAPRCASPARVKDIPLQDMPSFEFRCSELDDQGCLGEDYCPPKCTCTGTIVRCSRAKLREIPKNIPPETTELYLDVNEIQSIQSERLSHLRSLTRLDLSNNMVSVLSNNTFANLTKLSSLIVSYNKLQCVQRNAFSGLLSLRILSLHGNDVSMIPEGAFTDLQSITHLALGSNPFYCDCNLRWLAEWVKRDYVEPGIARCAEPTLLRDKLLLTSPADHFQCKGK-ISNEVLAKCDTCYTFPCKNGATCRPTGERDYECLCAPGYHGKLCESAIDACYGNPCRNSAICKVLEEGRFSCHCSAGYEGDRCEININDCLDHKCVNNGTCVDQVESNRCDCQSGYTGTYCEKKIQFCTKEFNPCKNGATCLDRG-DHYTCQCALGFSGENCTLNNDDCVNHMCQNGGTCIDGINEYTCKCPPEFSGKFCEASPMVSLLYPQTSPCQQHDCQNGICFQPQGLTDYICKCASGYTGKRCEFLTSVSFAQNGSYIEMPSLKTKPEANITMIFPTEEENGILIYNGESQHIAVELFRGRIRASYDVGNYPVSNMFSYETVSDGRYHTVELLSQKKNFTLRVDQGNARSMINEG 1287 HSP 2 Score: 310.842 bits (795), Expect = 3.104e-83 Identity = 208/677 (30.72%), Postives = 324/677 (47.86%), Query Frame = 0
Query: 119 VLELKH-GRGCRSDEDLLTAGESICLDS-RVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQK-----NPVETSG--ARCETPVKLQRKKISSLHLGRSNCLDLS---SASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRG------------------------LDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEG-------CLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWM--KNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAED 749
VL L H G + DE +AG+ D+ + C + C C VDC ++ L + P +P A ++ LE NN+ S+ F+ LR + LSNN+++ IPD F L L L L NQ+ + + L SL+ L L++N++ CI A + L +L+L +NN+ +L F LS L+ L L +N + C C LAWL +L++ +P+ +G RC P L+ K +S L C + S C+ CP C C++GVVDC + + IP +P + EL+L N + +P+ F L ++LS N IS I +F G +S+ L L N +T + + G L L L L++NNI + G FD L + ++ NP CDC++ W D+L + ++ C +P L+ R++ L RC + E C + CP EC C GT++ CS+ LK +P +P +EL L+ N I ++ F L L ++D+ N+IS I+ F L L ++ N I+ I F+GL LR LSL N I+ + +FD S+T L + SNPL C+C++ W W+ K Q + G +C+ P + + + P F+C D
Sbjct: 59 VLLLLHVAGGGQRDEPARSAGKGAGSDAATLPCSRLCQCEANAVDCSNRGLTQVPLDLPKDADKINLEGNNLTSLLSSDFEGFANLRILRLSNNQLQYIPDLVFATLGHLQRLDLSHNQLTAVGKRTLRGLNSLRNLQLDNNQLTCIDEAALRSQKELEILTLNNNNLTSLPRDLFEPLSRLRVLRLSENKWLCDCHLAWLGRFLRRHARAGSPLAANGYLPRCHAPFGLRIKAVSELLDTEFKCTGAAEKPSEECSAQPGCPLMCRCADGVVDCRDKSLSRIPPYLPDSATELRLEQNQITEIPAKA-FAAYKKLKRVDLSNNQISSIAGDAFHGLKSLTSLILYGNRITELPAGMFHGLTSLQLLLLNANRISCVRRDSFKDLHNLNLLSLYDNNIPTMLNGTFDSLRNIQTLHLGRNPFLCDCYLTWLADYLHRKPVETSGVRCESPRRLQRRRIAVLRDEKSRCSDRDLERNLLEISPCEDDPSCPAECSCKGTIVDCSNKGLKEIPRELPLYTTELILNDNQIVRIKADGMFRDLPNLLKVDLRRNQISAIENGAFEGANTLLDLNLAENIIQEIHNKMFTGLVSLRTLSLFQNKITCVTPGSFDYLRSLTSLNILSNPLHCNCHMAWFGDWLRKKEQELSTGAPRCASPARVKDIPLQDMPSFEFRCSELD 734
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Match: gi|321468289|gb|EFX79275.1| (hypothetical protein DAPPUDRAFT_304927 [Daphnia pulex]) HSP 1 Score: 1064.29 bits (2751), Expect = 0.000e+0 Identity = 515/968 (53.20%), Postives = 673/968 (69.52%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSAS-----CNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLR--SQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEG 1109
CP C C DG+VDCRDK L R P +P ATELRLEQN I IP KAF K L+R+DLSNN+I I D F GL LTSL+LYGN+I + F+ L SLQLLLLN+N+I C+ ++F L L+LLSLYDNNI T+ NGTF+ L ++QTLHLG+N F C C L WL D+L + PVETSG RCE+P ++QR++IS L +S C SS C CP CSC +VDCS +G+K IP ++P T EL L+DN + + + G+F LP L ++L RN IS IE G+FEGA ++ L+L+ N + I ++ GL L TL L N I C++PG+FD L L+ NILSNP+ C+CHMAWFGDWLR Q ++ CA+PA ++D LQ++P FRC +D+GCL + YCP +C CTGT++RCS A+L+ +P N+P E +ELYLDVN+I ++ SHL+ L+RLD+SNN +SV+ F NLT+LS+LI+SYN+++C+Q +AFSGL LRILSLHGN +SMIP AF D SITHLALGSNP CDCNL+WL++W+K Y+EPGIA+C+ P L + L+LT+P F+C+ + E++AKCD C++ PC+N ATCR ++ C C PGY+G +CE IDACYGNPC + C V E GR+ C C GY G RCE I++C + C NNATC D+V +RCDC GY+G C KI C + C NG +C+ + Y C C GFSG +C+V+ DDC SHMCQNGGTC+DG Y+C C PEF+GK+CE P++S +YPQ+SPCQQH+C++G CF P+ DY+CKC GYTGK CE+LTS+SF Q G+Y +M L TKP ANITM T E +G+L+Y G ++H+AVELF+GRIRVSYDVGNYPVS MFSYE +SDG H +++L+Q KN TLRVD G +RS++NEG
Sbjct: 273 CPLMCRCADGVVDCRDKSLSRIPPYLPDSATELRLEQNQITEIPAKAFAAYKKLKRVDLSNNQISSIAGDAFHGLKSLTSLILYGNRITELPAGMFHGLTSLQLLLLNANRISCVRRDSFKDLHNLNLLSLYDNNIPTMLNGTFDSLRNIQTLHLGRNPFLCDCYLTWLADYLHRKPVETSGVRCESPRRMQRRRISVLRDEKSRCSGKSSPPFLSPPCEDDPSCPTECSCKGTIVDCSNKGLKEIPRELPLYTTELILNDNQIVRIKADGMFRDLPNLLKVDLRRNQISAIENGAFEGANTLVDLNLAENMIQEIHNKMFTGLVSLRTLSLFQNKITCVTPGSFDYLRSLTSLNILSNPLHCNCHMAWFGDWLRKKEQELSTGAPRCASPARVKDIPLQDMPSFEFRCSELDDQGCLGEDYCPPKCTCTGTIVRCSRAKLREIPKNIPPETTELYLDVNEIQSIQSERLSHLRSLTRLDLSNNMVSVLSNNTFVNLTKLSSLIVSYNKLQCVQRNAFSGLLSLRILSLHGNDVSMIPEGAFTDLQSITHLALGSNPFYCDCNLRWLAEWVKRDYVEPGIARCAEPILLRDKLLLTSPADHFQCKGK-ISNEVLAKCDTCYTFPCKNGATCRPTGERDYECLCAPGYHGKLCESAIDACYGNPCRNSAICKVLEEGRFSCHCSAGYEGDRCETNINDCLDHKCVNNATCVDQVESYRCDCQSGYTGIYCEKKIQFCTKDFNPCKNGATCVDRG-DHYACQCALGFSGENCTVNNDDCISHMCQNGGTCVDGINEYTCKCPPEFSGKFCEASPMVSLLYPQTSPCQQHDCQNGICFQPQGLTDYICKCASGYTGKRCEFLTSVSFAQNGSYIEMPSLKTKPEANITMIFSTEEENGILIYNGESQHIAVELFRGRIRVSYDVGNYPVSNMFSYETVSDGRYHTVELLSQKKNFTLRVDQGNARSMINEG 1238 HSP 2 Score: 309.301 bits (791), Expect = 7.229e-83 Identity = 197/644 (30.59%), Postives = 308/644 (47.83%), Query Frame = 0
Query: 149 CPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQK-----NPVETSG--ARCETPVKLQRKKISSLHLGRSNCL----DLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRG------------------------LDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSE-----DEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWM--KNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAED 749
C + C C VDC ++ L + P +P A ++ LE NN+ S+ F+ LR + LSNN+++ IPD F L L L L NQ+ + + L SL+ L L++N++ CI A + L +L+L +NN+ +L F LS L+ L L +N + C C LAWL +L++ +P+ +G RC P L+ K +S L C + S C+ CP C C++GVVDC + + IP +P + EL+L N + +P+ F L ++LS N IS I +F G +S+ L L N +T + + G L L L L++NNI + G FD L + ++ NP CDC++ W D+L + ++ C +P ++ R++ L RC G C + CP EC C GT++ CS+ LK +P +P +EL L+ N I ++ F L L ++D+ N+IS I+ F L L ++ N I+ I F+GL LR LSL N I+ + +FD S+T L + SNPL C+C++ W W+ K Q + G +C+ P + + + P F+C D
Sbjct: 43 CSRLCQCEANAVDCSNRGLTQVPLDLPKDADKINLEGNNLTSLLSSDFEGFANLRILRLSNNQLQYIPDLVFATLGHLQRLDLSHNQLTAVGKRTLRGLNSLRNLQLDNNQLTCIDEAALRSQKELEILTLNNNNLTSLPRDLFEPLSRLRVLRLSENKWLCDCHLAWLGRFLRRHARAGSPLAANGYLPRCHAPFGLRIKAVSELLDTEFKCTGKTAERPSEECSAQPGCPLMCRCADGVVDCRDKSLSRIPPYLPDSATELRLEQNQITEIPAKA-FAAYKKLKRVDLSNNQISSIAGDAFHGLKSLTSLILYGNRITELPAGMFHGLTSLQLLLLNANRISCVRRDSFKDLHNLNLLSLYDNNIPTMLNGTFDSLRNIQTLHLGRNPFLCDCYLTWLADYLHRKPVETSGVRCESPRRMQRRRISVLRDEKSRCSGKSSPPFLSPPCEDDPSCPTECSCKGTIVDCSNKGLKEIPRELPLYTTELILNDNQIVRIKADGMFRDLPNLLKVDLRRNQISAIENGAFEGANTLVDLNLAENMIQEIHNKMFTGLVSLRTLSLFQNKITCVTPGSFDYLRSLTSLNILSNPLHCNCHMAWFGDWLRKKEQELSTGAPRCASPARVKDIPLQDMPSFEFRCSELD 685 HSP 3 Score: 221.09 bits (562), Expect = 1.067e-54 Identity = 136/408 (33.33%), Postives = 195/408 (47.79%), Query Frame = 0
Query: 148 NCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKA-FKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVE--TSGARCETPVKLQRKKISSLHLGRSNCLDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKG 552
+CP EC C IVDC +K L+ P +P TEL L N IV I F+ L L ++DL N+I I + F G L L L N I+ I F L SL+ L L NKI C+ P G+F+ L SL +L++ N C C +AW DWL+K E T RC +P +++ + + C +L C CP C+C+ +V CSR ++ IP +IP T EL L N + + S L +L +L+ L+LS N +SV+ +F + L +S+N L ++ GL L L LH N++ I GAF L ++ + SNP CDC++ W +W++ +A CA P LRD+ L P F+CKG
Sbjct: 496 SCPTECSCKGTIVDCSNKGLKEIPRELPLYTTELILNDNQIVRIKADGMFRDLPNLLKVDLRRNQISAIENGAFEGANTLVDLNLAENMIQEIHNKMFTGLVSLRTLSLFQNKITCVTP------------------------GSFDYLRSLTSLNILSNPLHCNCHMAWFGDWLRKKEQELSTGAPRCASPARVKDIPLQDMPSFEFRCSELDDQGCLGEDYCPPKCTCTGTIVRCSRAKLREIPKNIPPETTELYLDVNEIQSIQSERLSHLR-SLTRLDLSNNMVSVLSNNTFVNLTKLSSLIVSYNKLQCVQRNAFSGLLSLRILSLHGNDVSMIPEGAFTDLQSITHLALGSNPFYCDCNLRWLAEWVKRDYVEPGIARCAEPILLRDKLLLTSPADHFQCKG 878 HSP 4 Score: 219.164 bits (557), Expect = 5.068e-54 Identity = 141/465 (30.32%), Postives = 204/465 (43.87%), Query Frame = 0
Query: 367 PCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGA------------------------FDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSA-------LATCATPAHLRDRQLQELPLPSFRCKGSE----DEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLII------------------------SYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATC 772
PC C C VDCS RG+ +P D+PK ++ L N L + S F L L LS N + I + F + LDLSHN LT + R LRGL+ L L L NN + CI A F+ L +L + N CDCH+AW G +LR + L C P LR + + EL F+C G E C Q CP C C V+ C L +P +P A+EL L+ N I ++ F+ ++L R+D+SNN+IS I F L L++LI+ + N I C++ +F LH+L +LSL+ N+I + N FD +I L LG NP LCDC L WL+ ++ + +E +C P+ + + + +C + +P P S PC++ +C
Sbjct: 42 PCSRLCQCEANAVDCSNRGLTQVPLDLPKDADKINLEGNNLTSLLSSD-FEGFANLRILRLSNNQLQYIPDLVFATLGHLQRLDLSHNQLTAVGKRTLRGLNSLRNLQLDNNQLTCIDEAALRSQKELEILTLNNNNLTSLPRDLFEPLSRLRVLRLSENKWLCDCHLAWLGRFLRRHARAGSPLAANGYLPRCHAPFGLRIKAVSELLDTEFKCTGKTAERPSEECSAQPGCPLMCRCADGVVDCRDKSLSRIPPYLPDSATELRLEQNQITEIPAKAFAAYKKLKRVDLSNNQISSIAGDAFHGLKSLTSLILYGNRITELPAGMFHGLTSLQLLLLNANRISCVRRDSFKDLHNLNLLSLYDNNIPTMLNGTFDSLRNIQTLHLGRNPFLCDCYLTWLADYLHRKPVETSGVRCESPRRMQRRRISVLRDEKSRCSGKSSP--------PFLSPPCEDDPSC 497
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Match: gi|1060249055|ref|XP_017866874.1| (PREDICTED: protein slit isoform X2 [Drosophila arizonae]) HSP 1 Score: 1060.83 bits (2742), Expect = 0.000e+0 Identity = 560/1181 (47.42%), Postives = 741/1181 (62.74%), Query Frame = 0
Query: 148 NCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNC----LDLS-SASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDEC--ESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDS-KDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEGANANGTCSIIQRAPGFECQCKPGFSGFYCNQKKDLSSSFQS------------------------------DGSQREGKKGNKKN---------------------REGGRRVRNKRKK---RCK-KQKYRDYYIDNSG----------------CRSKRPYKMAKCVSDAADSCVAKK-TKLRTIRFVCENGRTFNKEVEMIRKCGRIKK 1248
+CP C C DGIVDCR+K L P ++P TELRLEQN I +P K+F + LRRIDLSNN I I D +GL +LT+LVLYGN+I+ + F L SLQLLLLN+N+I CI +AF L L LLSLYDNNIQ+L NGTF+ + S++T+HL KN F C C+L WL D+L KNP+ETSGARCE+P ++ R++I SL + C L + S C S CP C C VDC+ RG+K IP DIP T EL L+DN L + S GLF LP L L L RN ++ IE +FEGA I L L N + I ++ GL L TL+L++N I C+ PG+F+ L L+ N+ SNP C+CH+AWF +WLR ++ + A CA PA +RD Q+++LP F+C +EGCL YCP C CTGTV+RCS QLK +P +P E SELYL+ N+I+ + HL+ L+RLD+SNN+I+++ Y FANLT+LSTLIISYN+++C+Q HA SGL++LR+LSLHGN ISM+P +F+D S+TH+ALGSNPL CDC+LKW S W+K Y+EPGIA+C+ P+ + + L+L+TP S+F C+ + EI+AKC+ C+ PCQNKA C A ++ C C PGY+G CE IDACYGNPC + TCTV E GR+ C C PGY+G RCE ID+C E CQNNATC D V +RC+C G+SGE C KI C + C NG CL + Y+C C+ GF G +C+ ++DDC++HMCQNGGTC+DG Y C C +F GKYCE +IS MYPQ+SPCQ HECKHG CF P + DY+CKC PGYTGK+CEYLTS+SF ++ ++EPL T+P AN+T+ + E +G+L+Y G++ HLAVELF GRIRVSYDVGNYPVSTM+S+E ++DG H +++LA KN TLRVD G +RSI+NEG+N S G + P + Q ++L +SF+ +G EG++ ++++ R G R V N K +CK K R Y D + CRS++P K AKCV + C A K + R +R VC N R + K ++++RKCG KK
Sbjct: 341 SCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPAKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLSSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPSMCQCEGTTVDCAGRGLKEIPRDIPLHTTELLLNDNELGRINSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASRIQDLQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLSGGAARCAAPAKVRDVQIKDLPHNEFKCSSDNNEGCLGDGYCPPACTCTGTVVRCSRNQLKEIPRGIPPETSELYLESNEIEQIHYERIRHLRALTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCSLKWFSDWIKLDYVEPGIARCAEPEHMKDKLILSTPSSNFVCRGK-VRNEILAKCNACYEQPCQNKAQCLALPQRDYQCLCQPGYHGRHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEDIRCQNNATCIDGVQSYRCECQPGFSGEWCDTKIQFCSQEFNPCANGAKCL-DHFSHYSCECMAGFHGINCTDNIDDCQNHMCQNGGTCIDGINDYVCKCPDDFTGKYCEGHNLISMMYPQTSPCQNHECKHGVCFQPNAAGSDYLCKCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNYPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSNDYLKLSTPMFLGGLPVE--PAQQAYKNWQIRNL-TSFKGCMKEVWINHKLVDFGNAQRQQKITPGCALLEGDPSEGEEDDEQDFMDETPHIKEEPVDPCSEHKCRRGSRCVPNANSKDGYQCKCKHGQRGRYCDQAAATCRKEQVREYYTENDCRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKK 1516 HSP 2 Score: 323.939 bits (829), Expect = 1.102e-87 Identity = 195/668 (29.19%), Postives = 325/668 (48.65%), Query Frame = 0
Query: 138 GESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLR------------------------RIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSAS---CNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNL-----------------------LPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSE-----DEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAED 749
G + + + CP+ C C+ VDC + L + P I L L+ NN+ I F++L LR R+ L+NN ++ IP++ T L L + N I + F SL+ L L++N+I C+ +AF L L +L+L +NN+ L + F GL L+ L L N FAC C L+WL+ +L+ P RC++P +L+ + ++ LH C L+ + C + CP PC C++G+VDC + + +P +P T EL+L N + +P+ + L L+TL L N I + G F+G S+ +L L+ N ++ IR R L L+ L L++NNI ++ G FD + + ++ NP CDC++ W D+L ++ A C +P + R+++ L F+C E C CP C C GT + C+ LK +P ++P +EL L+ N++ + F L L +L++ N+++ I+P F +R+ L + N+IK I F GLH L+ L+L+ N IS + +F+ NS+T L L SNP C+C+L W ++W++ + + G A+C+ P + + + P + FKC +++
Sbjct: 85 GGGVGVITEARCPRVCSCSGLTVDCSHRGLTQVPRKISADVERLDLQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIEKNALQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISHNVITTVGRRVFKGAQSLRSLQLDNNQITCMDEHAFKGLLELEILTLNNNNLTALPHNAFGGLGKLRALRLSDNPFACDCHLSWLSRYLRSAPRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGVENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPAKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLSSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPSMCQCEGTTVDCAGRGLKEIPRDIPLHTTELLLNDNELGRINSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASRIQDLQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLSGGAARCAAPAKVRDVQIKDLPHNEFKCSSDN 752
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Match: gi|1060249065|ref|XP_017866877.1| (PREDICTED: protein slit isoform X4 [Drosophila arizonae]) HSP 1 Score: 1060.44 bits (2741), Expect = 0.000e+0 Identity = 560/1181 (47.42%), Postives = 741/1181 (62.74%), Query Frame = 0
Query: 148 NCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNC----LDLS-SASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDEC--ESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDS-KDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEGANANGTCSIIQRAPGFECQCKPGFSGFYCNQKKDLSSSFQS------------------------------DGSQREGKKGNKKN---------------------REGGRRVRNKRKK---RCK-KQKYRDYYIDNSG----------------CRSKRPYKMAKCVSDAADSCVAKK-TKLRTIRFVCENGRTFNKEVEMIRKCGRIKK 1248
+CP C C DGIVDCR+K L P ++P TELRLEQN I +P K+F + LRRIDLSNN I I D +GL +LT+LVLYGN+I+ + F L SLQLLLLN+N+I CI +AF L L LLSLYDNNIQ+L NGTF+ + S++T+HL KN F C C+L WL D+L KNP+ETSGARCE+P ++ R++I SL + C L + S C S CP C C VDC+ RG+K IP DIP T EL L+DN L + S GLF LP L L L RN ++ IE +FEGA I L L N + I ++ GL L TL+L++N I C+ PG+F+ L L+ N+ SNP C+CH+AWF +WLR ++ + A CA PA +RD Q+++LP F+C +EGCL YCP C CTGTV+RCS QLK +P +P E SELYL+ N+I+ + HL+ L+RLD+SNN+I+++ Y FANLT+LSTLIISYN+++C+Q HA SGL++LR+LSLHGN ISM+P +F+D S+TH+ALGSNPL CDC+LKW S W+K Y+EPGIA+C+ P+ + + L+L+TP S+F C+ + EI+AKC+ C+ PCQNKA C A ++ C C PGY+G CE IDACYGNPC + TCTV E GR+ C C PGY+G RCE ID+C E CQNNATC D V +RC+C G+SGE C KI C + C NG CL + Y+C C+ GF G +C+ ++DDC++HMCQNGGTC+DG Y C C +F GKYCE +IS MYPQ+SPCQ HECKHG CF P + DY+CKC PGYTGK+CEYLTS+SF ++ ++EPL T+P AN+T+ + E +G+L+Y G++ HLAVELF GRIRVSYDVGNYPVSTM+S+E ++DG H +++LA KN TLRVD G +RSI+NEG+N S G + P + Q ++L +SF+ +G EG++ ++++ R G R V N K +CK K R Y D + CRS++P K AKCV + C A K + R +R VC N R + K ++++RKCG KK
Sbjct: 317 SCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPAKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLSSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPSMCQCEGTTVDCAGRGLKEIPRDIPLHTTELLLNDNELGRINSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASRIQDLQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLSGGAARCAAPAKVRDVQIKDLPHNEFKCSSDNNEGCLGDGYCPPACTCTGTVVRCSRNQLKEIPRGIPPETSELYLESNEIEQIHYERIRHLRALTRLDLSNNQITILSNYTFANLTKLSTLIISYNKLQCLQRHALSGLNNLRVLSLHGNRISMLPEGSFEDLKSLTHIALGSNPLYCDCSLKWFSDWIKLDYVEPGIARCAEPEHMKDKLILSTPSSNFVCRGK-VRNEILAKCNACYEQPCQNKAQCLALPQRDYQCLCQPGYHGRHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGEDIRCQNNATCIDGVQSYRCECQPGFSGEWCDTKIQFCSQEFNPCANGAKCL-DHFSHYSCECMAGFHGINCTDNIDDCQNHMCQNGGTCIDGINDYVCKCPDDFTGKYCEGHNLISMMYPQTSPCQNHECKHGVCFQPNAAGSDYLCKCHPGYTGKWCEYLTSISFVHNNSFVELEPLRTRPEANVTIVFSSAEQNGILMYDGQDAHLAVELFNGRIRVSYDVGNYPVSTMYSFEMVADGKYHAVELLAIKKNFTLRVDRGLARSIINEGSNDYLKLSTPMFLGGLPVE--PAQQAYKNWQIRNL-TSFKGCMKEVWINHKLVDFGNAQRQQKITPGCALLEGDPSEGEEDDEQDFMDETPHIKEEPVDPCSEHKCRRGSRCVPNANSKDGYQCKCKHGQRGRYCDQAAATCRKEQVREYYTENDCRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKK 1492 HSP 2 Score: 332.028 bits (850), Expect = 2.110e-90 Identity = 193/644 (29.97%), Postives = 322/644 (50.00%), Query Frame = 0
Query: 138 GESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSAS---CNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNL-----------------------LPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSE-----DEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAED 749
G + + + CP+ C C+ VDC + L + P I L L+ NN+ I F++L LR + L++N+I I + L L L + N I + F SL+ L L++N+I C+ +AF L L +L+L +NN+ L + F GL L+ L L N FAC C L+WL+ +L+ P RC++P +L+ + ++ LH C L+ + C + CP PC C++G+VDC + + +P +P T EL+L N + +P+ + L L+TL L N I + G F+G S+ +L L+ N ++ IR R L L+ L L++NNI ++ G FD + + ++ NP CDC++ W D+L ++ A C +P + R+++ L F+C E C CP C C GT + C+ LK +P ++P +EL L+ N++ + F L L +L++ N+++ I+P F +R+ L + N+IK I F GLH L+ L+L+ N IS + +F+ NS+T L L SNP C+C+L W ++W++ + + G A+C+ P + + + P + FKC +++
Sbjct: 85 GGGVGVITEARCPRVCSCSGLTVDCSHRGLTQVPRKISADVERLDLQGNNLTVIYETDFQRLTKLRMLQLTDNQIHTIEKNALQDLVSLERLDISHNVITTVGRRVFKGAQSLRSLQLDNNQITCMDEHAFKGLLELEILTLNNNNLTALPHNAFGGLGKLRALRLSDNPFACDCHLSWLSRYLRSAPRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTEHAPMECGVENSCPHPCRCADGIVDCREKSLTSVPVTLPDDTTELRLEQNFITELPAKSFSSFRRLRRIDLSNNNISRIAHDALSGLKQLTTLVLYGNKIKDLPSGVFKGLSSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIKTVHLAKNPFICDCNLRWLADYLHKNPIETSGARCESPKRMHRRRIESLREEKFKCSWDELRMKLSGECRMDSDCPSMCQCEGTTVDCAGRGLKEIPRDIPLHTTELLLNDNELGRINSDGLFGRLPHLVKLELKRNQLTGIEPNAFEGASRIQDLQLGENKIKEISNKMFLGLHQLKTLNLYDNQISCVMPGSFEHLNSLTSLNLASNPFNCNCHLAWFAEWLRKKSLSGGAARCAAPAKVRDVQIKDLPHNEFKCSSDN 728
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Match: gi|1048015052|ref|XP_017468504.1| (PREDICTED: protein slit isoform X2 [Rhagoletis zephyria]) HSP 1 Score: 1060.06 bits (2740), Expect = 0.000e+0 Identity = 558/1176 (47.45%), Postives = 733/1176 (62.33%), Query Frame = 0
Query: 148 NCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNC------LDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDEC-ESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDS-KDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEGANANGTCSIIQRAPGFECQCKPGFSGFYCNQKKDLSS---------------SFQSDGSQREGKKG------------------------------NKKNREGGRRVRNKRKK-----RCK----------------KQKYRDYYIDNSGCRSKRPYKMAKCVSDAADSCVAKK-TKLRTIRFVCENGRTFNKEVEMIRKCGRIKK 1248
+CP C C DGIVDCR+K L P S+P TELRLEQN I +PPKAF + LRRIDLSNN I I D GL LT+LVLYGN+I+ + F L SLQLLLLN+N+I CI +AF L L LLSLYDNNIQ+L NGTF+ + S+QTLHL KN F C C+L WL D+L KNP+ETSGARCE P ++ R++I SL + C + LSS C CP C C +DCS RG+K IP DIP T EL LSDN L + S GLF LP L L+L RN I+ IE +FEGA I L ++ N + I ++ GL L TL+L+NN I C+ PG+F+ L L+ N+ +NP C+CH+AWF +WLR + A CA P+ +RD Q+++LP F+C +EGCL YCP C CTGTV+RCS QLK +P +P E SELYL+ N+I + + HL+ L+RLD+SNN+I+ + Y FANLT LSTLIISYN ++C+Q +A +GL +LR+LSLHGN ISM+P +F+D S+TH+ALGSNPL CDC+LKW S W+K Y+EPGIA+C+ P + + L+L+TP +F C+ + EI+AKCD C++ PC+NKA C ++ C C PGY+G CE IDACYGNPC + TCTV E GR+ C C PGY+G RCE ID+C CQNNATC D V + C+C G++G+ C KI+ C + + C NG C YTC C+ GF G +C+ ++DDC++H+CQNGGTC+DG Y C C +F GKYCE ++S MYPQ+SPCQ HECKHG CF P S DY+CKC PGYTGK+CEYLTS+SF ++ +MEPL TKP AN+T+ + E +G+L+Y G++ H+AVELF GRIRVSYDVGNYPVSTM+S+E ++DG H +++LA KN TLRVD G +RSI+NEG+N + G + PG + Q ++L+S F++ Q++ G N K R G R V N + +CK K++ R+YY +N CRS++P K AKCV + C A K + R +R VC N R + K ++++RKCG KK
Sbjct: 463 SCPHPCRCADGIVDCREKSLTSVPVSLPDDTTELRLEQNFITELPPKAFANFRRLRRIDLSNNNISRIAHDALAGLKALTTLVLYGNKIKDLPSGVFKGLTSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIQTLHLAKNPFICDCNLRWLADYLHKNPIETSGARCEAPKRMHRRRIESLREEKFKCSWDDMRIKLSS-ECRADVDCPAMCQCDGTSIDCSGRGLKEIPRDIPLHTTELLLSDNELGRIKSDGLFGRLPHLVKLDLRRNHITGIEPNAFEGANRIQELVMAENKIREISNKMFLGLHQLKTLNLYNNQISCVMPGSFEFLSSLTSLNLAANPFNCNCHLAWFSEWLRKKGLIGGAARCAAPSKVRDMQIKDLPHNEFKCTSDNNEGCLGDGYCPPACTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEITMIHLDRIRHLKSLTRLDLSNNQITFLSNYTFANLTNLSTLIISYNNLQCLQRYALAGLQNLRVLSLHGNRISMLPEGSFEDMKSLTHIALGSNPLYCDCSLKWFSDWIKLDYVEPGIARCAEPDYMKDKLILSTPSHNFMCKGK-VSNEIMAKCDACYTHPCENKAQCIPLPQRDYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGETKCQNNATCVDGVESYTCECQPGFTGQYCDTKINFCSDEFNPCANGAKC-HDHFTHYTCECMAGFHGINCTQNIDDCQNHICQNGGTCIDGINDYVCKCPDDFTGKYCEGHNMVSMMYPQTSPCQNHECKHGVCFQPNPSGSDYLCKCHPGYTGKWCEYLTSISFVHNNSFVEMEPLRTKPDANVTIVFSSSEQNGILMYDGQDAHIAVELFNGRIRVSYDVGNYPVSTMYSFEMVADGKYHCVELLAIKKNFTLRVDRGLARSIINEGSNDFLKLTTPMFLGGLPPE--PGQQAYKNWQIRNLTSFKGCMKEVWINRKLVDFENAARQQKITPGCALLEGEAAEDDEQDFMDETPHIKEVDPCENHKCRRGSRCVPNSTYRDGYQCKCKQGQKGRYCEQVTSTCRKEQVREYYTEND-CRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKK 1632 HSP 2 Score: 326.635 bits (836), Expect = 2.201e-88 Identity = 200/668 (29.94%), Postives = 328/668 (49.10%), Query Frame = 0
Query: 138 GESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIV------------------------SIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSAS---CNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNL-----------------------LPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSE-----DEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAED 749
G S+ + S CP+ C CN VDC + L + P I L L+ NNI +I AF+ L L R+ L+NN ++ IP++ + L L + N I + F SL+ L L++N+I C+ +AF L L +L+L +NN+ +L + F+GL+ L+ L L N F+C C L+WL+ +L+ P RC++P +L+ + ++ LH C L+ + C + CP PC C++G+VDC + + +P +P T EL+L N + +P N L AL+TL L N I + G F+G S+ +L L+ N ++ IR R L L+ L L++NNI ++ G FD + + ++ NP CDC++ W D+L ++ A C P + R+++ L F+C + C CP C C GT I CS LK +P ++P +EL L N++ ++ F L L +LD+ N I+ I+P F R+ L+++ N+I+ I F GLH L+ L+L+ N IS + +F+ +S+T L L +NP C+C+L W S+W++ + + G A+C+ P + + + P + FKC +++
Sbjct: 207 GGSLGVISESRCPRVCSCNGLTVDCSHRGLTQVPRKISADVERLDLQGNNITVIFEADFQRLTKLRILQLTDNQIHTIEKGAFQDLVSLERLRLNNNLLKAIPENLVSSSASLLRLDISHNIITTVGRRVFKGAQSLRSLQLDNNQITCMDEHAFKGLIDLEILTLNNNNLTSLPHNAFSGLNRLRALRLSDNPFSCDCHLSWLSRFLRSAPRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTENAPMECGMENSCPHPCRCADGIVDCREKSLTSVPVSLPDDTTELRLEQNFITELPPKAFANFRRLRRIDLSNNNISRIAHDALAGLKALTTLVLYGNKIKDLPSGVFKGLTSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIQTLHLAKNPFICDCNLRWLADYLHKNPIETSGARCEAPKRMHRRRIESLREEKFKCSWDDMRIKLSSECRADVDCPAMCQCDGTSIDCSGRGLKEIPRDIPLHTTELLLSDNELGRIKSDGLFGRLPHLVKLDLRRNHITGIEPNAFEGANRIQELVMAENKIREISNKMFLGLHQLKTLNLYNNQISCVMPGSFEFLSSLTSLNLAANPFNCNCHLAWFSEWLRKKGLIGGAARCAAPSKVRDMQIKDLPHNEFKCTSDN 874
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Match: gi|1048015056|ref|XP_017468506.1| (PREDICTED: protein slit isoform X4 [Rhagoletis zephyria]) HSP 1 Score: 1058.9 bits (2737), Expect = 0.000e+0 Identity = 558/1176 (47.45%), Postives = 734/1176 (62.41%), Query Frame = 0
Query: 148 NCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNC------LDLSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDEC-ESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPKDS-KDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEGANANGTCSIIQRAPGFECQCKPGFSGFYCNQKKDLSS---------------SFQSDGSQREGKKG------------------------------NKKNREGGRRVRNKRKK-----RCK----------------KQKYRDYYIDNSGCRSKRPYKMAKCVSDAADSCVAKK-TKLRTIRFVCENGRTFNKEVEMIRKCGRIKK 1248
+CP C C DGIVDCR+K L P S+P TELRLEQN I +PPKAF + LRRIDLSNN I I D GL LT+LVLYGN+I+ + F L SLQLLLLN+N+I CI +AF L L LLSLYDNNIQ+L NGTF+ + S+QTLHL KN F C C+L WL D+L KNP+ETSGARCE P ++ R++I SL + C + LSS C CP C C +DCS RG+K IP DIP T EL LSDN L + S GLF LP L L+L RN I+ IE +FEGA I L ++ N + I ++ GL L TL+L+NN I C+ PG+F+ L L+ N+ +NP C+CH+AWF +WLR + A CA P+ +RD Q+++LP F+C +EGCL YCP C CTGTV+RCS QLK +P +P E SELYL+ N+I + + HL+ L+RLD+SNN+I+ + Y FANLT LSTLIISYN ++C+Q +A +GL +LR+LSLHGN ISM+P +F+D S+TH+ALGSNPL CDC+LKW S W+K Y+EPGIA+C+ P + + L+L+TP +F C+ + EI+AKCD C++ PC+NKA C ++ C C PGY+G CE IDACYGNPC + TCTV E GR+ C C PGY+G RCE ID+C CQNNATC D V + C+C G++G+ C KI+ C + + C NG C + YTC C+ GF G +C+ ++DDC++H+CQNGGTC+DG Y C C +F GKYCE ++S MYPQ+SPCQ HECKHG CF P S DY+CKC PGYTGK+CEYLTS+SF ++ +MEPL TKP AN+T+ + E +G+L+Y G++ H+AVELF GRIRVSYDVGNYPVSTM+S+E ++DG H +++LA KN TLRVD G +RSI+NEG+N + G + PG + Q ++L+S F++ Q++ G N K R G R V N + +CK K++ R+YY +N CRS++P K AKCV + C A K + R +R VC N R + K ++++RKCG KK
Sbjct: 439 SCPHPCRCADGIVDCREKSLTSVPVSLPDDTTELRLEQNFITELPPKAFANFRRLRRIDLSNNNISRIAHDALAGLKALTTLVLYGNKIKDLPSGVFKGLTSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIQTLHLAKNPFICDCNLRWLADYLHKNPIETSGARCEAPKRMHRRRIESLREEKFKCSWDDMRIKLSS-ECRADVDCPAMCQCDGTSIDCSGRGLKEIPRDIPLHTTELLLSDNELGRIKSDGLFGRLPHLVKLDLRRNHITGIEPNAFEGANRIQELVMAENKIREISNKMFLGLHQLKTLNLYNNQISCVMPGSFEFLSSLTSLNLAANPFNCNCHLAWFSEWLRKKGLIGGAARCAAPSKVRDMQIKDLPHNEFKCTSDNNEGCLGDGYCPPACTCTGTVVRCSRNQLKEIPRGIPAETSELYLESNEITMIHLDRIRHLKSLTRLDLSNNQITFLSNYTFANLTNLSTLIISYNNLQCLQRYALAGLQNLRVLSLHGNRISMLPEGSFEDMKSLTHIALGSNPLYCDCSLKWFSDWIKLDYVEPGIARCAEPDYMKDKLILSTPSHNFMCKGK-VSNEIMAKCDACYTHPCENKAQCIPLPQRDYQCLCQPGYHGKHCEFMIDACYGNPCRNNATCTVLEEGRFSCQCAPGYTGARCETNIDDCLGETKCQNNATCVDGVESYTCECQPGFTGQYCDTKINFCSDEFNPCANGAKCHDHFTH-YTCECMAGFHGINCTQNIDDCQNHICQNGGTCIDGINDYVCKCPDDFTGKYCEGHNMVSMMYPQTSPCQNHECKHGVCFQPNPSGSDYLCKCHPGYTGKWCEYLTSISFVHNNSFVEMEPLRTKPDANVTIVFSSSEQNGILMYDGQDAHIAVELFNGRIRVSYDVGNYPVSTMYSFEMVADGKYHCVELLAIKKNFTLRVDRGLARSIINEGSNDFLKLTTPMFLGGLPPE--PGQQAYKNWQIRNLTSFKGCMKEVWINRKLVDFENAARQQKITPGCALLEGEAAEDDEQDFMDETPHIKEVDPCENHKCRRGSRCVPNSTYRDGYQCKCKQGQKGRYCEQVTSTCRKEQVREYYTEND-CRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNRKYIKNLDIVRKCGCTKK 1608 HSP 2 Score: 337.421 bits (864), Expect = 6.537e-92 Identity = 200/644 (31.06%), Postives = 325/644 (50.47%), Query Frame = 0
Query: 138 GESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSAS---CNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNL-----------------------LPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSE-----DEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAED 749
G S+ + S CP+ C CN VDC + L + P I L L+ NNI I F++L LR + L++N+I I F L L L + N I + F SL+ L L++N+I C+ +AF L L +L+L +NN+ +L + F+GL+ L+ L L N F+C C L+WL+ +L+ P RC++P +L+ + ++ LH C L+ + C + CP PC C++G+VDC + + +P +P T EL+L N + +P N L AL+TL L N I + G F+G S+ +L L+ N ++ IR R L L+ L L++NNI ++ G FD + + ++ NP CDC++ W D+L ++ A C P + R+++ L F+C + C CP C C GT I CS LK +P ++P +EL L N++ ++ F L L +LD+ N I+ I+P F R+ L+++ N+I+ I F GLH L+ L+L+ N IS + +F+ +S+T L L +NP C+C+L W S+W++ + + G A+C+ P + + + P + FKC +++
Sbjct: 207 GGSLGVISESRCPRVCSCNGLTVDCSHRGLTQVPRKISADVERLDLQGNNITVIFEADFQRLTKLRILQLTDNQIHTIEKGAFQDLVSLERLDISHNIITTVGRRVFKGAQSLRSLQLDNNQITCMDEHAFKGLIDLEILTLNNNNLTSLPHNAFSGLNRLRALRLSDNPFSCDCHLSWLSRFLRSAPRLAPYTRCQSPSQLKGQNVADLHDQEFKCSGLTENAPMECGMENSCPHPCRCADGIVDCREKSLTSVPVSLPDDTTELRLEQNFITELPPKAFANFRRLRRIDLSNNNISRIAHDALAGLKALTTLVLYGNKIKDLPSGVFKGLTSLQLLLLNANEISCIRKDAFRDLHSLSLLSLYDNNIQSLANGTFDAMKSIQTLHLAKNPFICDCNLRWLADYLHKNPIETSGARCEAPKRMHRRRIESLREEKFKCSWDDMRIKLSSECRADVDCPAMCQCDGTSIDCSGRGLKEIPRDIPLHTTELLLSDNELGRIKSDGLFGRLPHLVKLDLRRNHITGIEPNAFEGANRIQELVMAENKIREISNKMFLGLHQLKTLNLYNNQISCVMPGSFEFLSSLTSLNLAANPFNCNCHLAWFSEWLRKKGLIGGAARCAAPSKVRDMQIKDLPHNEFKCTSDN 850
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Match: gi|170036234|ref|XP_001845970.1| (slit protein [Culex quinquefasciatus] >gi|167878768|gb|EDS42151.1| slit protein [Culex quinquefasciatus]) HSP 1 Score: 1056.97 bits (2732), Expect = 0.000e+0 Identity = 514/982 (52.34%), Postives = 678/982 (69.04%), Query Frame = 0
Query: 135 LTAGESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLD-----LSSASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPK-DSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEGA 1110
LT + R CP C C DGIVDCR+K L PA++P TELRLEQN I IPPKAF + L+RIDLSNN I + D F+GL LTSLVLYGN+I+ + S F L SLQLLLLN+N+I C+ +AF L+ L LLSLYDNNIQTL NGTF+ L S+QTLHL +N F C C+L WL D+L +NP+ETSGARC++P ++QR++I +L + C + + C CP C C VDCS RG+K IP DIP T EL L+DN L+ + S GLF LP L L+L RN IS +E +FEGA I L +S N + + ++ GL L TL L++N I C+ PG+F+ L L+ N+ SNP +C+CH+ WF DWLR + A CA+PA +RD +++LP F+C D+GCL + YCP C CTGTV+RCS +LK +P ++P E +ELYL+ N+I ++M+ SHL+ L+RLD+SNNKIS++ + FANL+RLSTLIISYN ++C+Q +A +GL +L++LSLHGN ISMIP+ +F D +ITH+ALGSNPL CDC+LKWLS+W+K Y+EPGIA+C+ P ++ + LVL+TP + F C+ + EI++KCD C++ PC+N A C+A ++ C C PGY+G CE IDACYGNPC + GTCTV E GR+ C C GYSG RCE ID+C + C NNATC D V + C C GY+G C +KI+ C + C NG C+ + + Y+C CVPG+ G +C+ ++DDC +HMCQNGGTCLDG Y C C EF GK+CE P+++ +YPQ+SPCQQHECK G CF P S DY+CKC PGY+GK CEYLTSL+F ++ ++EPL TKP AN+T+ + + +GVL+Y G NEHLAVELF GRIRVSYDVGNYPVSTM+S+E ++DG H +++LA KN TLRVD G +RSI+NEG+
Sbjct: 246 LTENAPMECGGRSLCPHPCRCADGIVDCREKSLTSVPATLPEDTTELRLEQNYITEIPPKAFAAHRRLKRIDLSNNNISRVAYDAFSGLKSLTSLVLYGNKIKDLPASVFKGLTSLQLLLLNANEITCVRKDAFKDLQNLSLLSLYDNNIQTLANGTFDALKSIQTLHLARNPFICDCNLRWLGDYLHQNPIETSGARCDSPKRMQRRRIEALKDEKFKCTEDHAKIKYAGECRMDQECPAACHCDRTTVDCSSRGLKEIPRDIPLYTTELLLNDNELNRIRSDGLFGRLPNLVKLDLRRNQISAVEPNAFEGATKIQELFISENKIPEVHNKMFLGLHQLKTLSLYDNLITCVMPGSFEFLSSLTQLNLASNPFRCNCHLGWFSDWLRKKQLGGPPARCASPAKVRDVPVKDLPHFEFKCTSDADQGCLGEGYCPPSCTCTGTVVRCSRNKLKEIPKSIPSETTELYLESNEISMIQMSRISHLKALTRLDLSNNKISMLSNHTFANLSRLSTLIISYNNLQCVQQYALAGLKNLKVLSLHGNHISMIPDGSFADLQAITHIALGSNPLYCDCSLKWLSEWVKRDYVEPGIARCAEPDAMKDKLVLSTPAAQFVCKGK-VSNEILSKCDACYTFPCKNNAVCKALPERQYECQCPPGYHGAHCEFMIDACYGNPCRNNGTCTVMEEGRFSCQCMSGYSGARCEINIDDCTGHKCLNNATCVDGVNSYSCGCLPGYTGPYCESKIEFCGPDFNPCQNGAKCVDHSTH-YSCECVPGYRGINCTDNIDDCVNHMCQNGGTCLDGINDYVCKCPSEFTGKFCEGTPMVAMLYPQTSPCQQHECKFGVCFQPNPSSADYICKCAPGYSGKRCEYLTSLTFLHNNSFVELEPLRTKPEANVTIVFSSTQQNGVLMYDGNNEHLAVELFNGRIRVSYDVGNYPVSTMYSFEMVADGKYHMVELLAIKKNFTLRVDRGLARSIINEGS 1225 HSP 2 Score: 321.242 bits (822), Expect = 7.532e-87 Identity = 187/603 (31.01%), Postives = 302/603 (50.08%), Query Frame = 0
Query: 181 LRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSAS---CNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDL------------------------SHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEG-------CLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAE 748
L+L N I +I A L L R+ L++N ++ IPD+ + L L L N + I +F SL+ L L++N+I C+ A L L +L+L +NNI TL F G+ L+ L L +N FAC C L+WL +L+ P RC +P +L+ + ++ LH C L+ + C S CP PC C++G+VDC + + +P+ +P+ T EL+L N + +P F L ++LS N IS + +F G +S+ L L + N +T +R + L L+ L L++NNI ++ G FD L + ++ NP CDC++ W GD+L ++ A C +P ++ R+++ L F+C +ED C CP C C T + CS LK +P ++P +EL L+ N+++ + F L L +LD+ N+IS ++P F T++ L IS N+I + F GLH L+ LSL+ N I+ + +F+ +S+T L L SNP C+C+L W S W++ + + A+C+ P + + V P FKC ++
Sbjct: 70 LQLTDNQIYTIEKDALLDLISLERLRLNSNRLKSIPDNFLSSAANLLRLDLSHNALAAIPKRAFKGATSLRSLQLDNNQITCLDEQAIKGLAELEILTLNNNNITTLARDMFTGMPRLRALRLSENPFACDCHLSWLARYLKNAPRLAPYTRCHSPSQLKGQNVADLHDQEFKCSGLTENAPMECGGRSLCPHPCRCADGIVDCREKSLTSVPATLPEDTTELRLEQNYITEIPPKA-FAAHRRLKRIDLSNNNISRVAYDAFSGLKSLTSLVLYGNKIKDLPASVFKGLTSLQLLLLNANEITCVRKDAFKDLQNLSLLSLYDNNIQTLANGTFDALKSIQTLHLARNPFICDCNLRWLGDYLHQNPIETSGARCDSPKRMQRRRIEALKDEKFKC--TEDHAKIKYAGECRMDQECPAACHCDRTTVDCSSRGLKEIPRDIPLYTTELLLNDNELNRIRSDGLFGRLPNLVKLDLRRNQISAVEPNAFEGATKIQELFISENKIPEVHNKMFLGLHQLKTLSLYDNLITCVMPGSFEFLSSLTQLNLASNPFRCNCHLGWFSDWLRKKQLGGPPARCASPAKVRDVPVKDLPHFEFKCTSD 669 HSP 3 Score: 65.855 bits (159), Expect = 5.912e-7 Identity = 39/126 (30.95%), Postives = 62/126 (49.21%), Query Frame = 0
Query: 1124 GFECQCKPGFSGFYCNQKKDLSSSFQSDGSQREGKKGNKKNREGGRRVRNKRKKRCKKQKYRDYYIDNSGCRSKRPYKMAKCVSDAADSCVAKK-TKLRTIRFVCENGRTFNKEVEMIRKCGRIKK 1248
G+ C+CK G G +C+Q EG + + + + C+K++ R+YY +N CRS++P K AKCV + C A K + R +R VC N + K ++++RKC KK
Sbjct: 1336 GYSCKCKNGAKGKFCDQG--------------EGSVSVVVDEDLPFKAPSSGSSTCRKEQVREYYTENE-CRSRQPLKYAKCVGGCGNQCCAAKIVRRRKVRMVCSNNTKYIKHLDIVRKCQCTKK 1446
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Match: gi|1218260673|ref|XP_021709128.1| (LOW QUALITY PROTEIN: protein slit [Aedes aegypti]) HSP 1 Score: 1054.66 bits (2726), Expect = 0.000e+0 Identity = 515/982 (52.44%), Postives = 671/982 (68.33%), Query Frame = 0
Query: 135 LTAGESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSS-----ASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPK-DSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEGA 1110
LT + R CP C C DGIVDCR+K L P+++P TELRLEQN I IPPKAF + L+RIDLSNN I + D F+GL LTSLVLYGN+I+ + S F L SLQLLLLN+N+I C+ +AF L+ L LLSLYDNNIQTL NGTF+ L S+QTLHL +N F C C+L WL D+L +NP+ETSGARC++P ++QR++I +L + C + S C CP C C VDCS RG+K IP DIP T EL L+DN L V S GLF LP L L+L RN I+ IE +FEGA I L +S N + + ++ GL L TL L++N I C+ PG+FD L L+ N+ SNP +C+CH+ WF DWLR + A CA+PA +RD +++LP F+C D+GCL + YCP C CTGTV+RCS +LK +P ++P E +ELYL+ NDI + SHL+ L+RLD+SNNKI ++ + F NL+RLSTLIISYN ++CIQ +A +GL L++LSLHGN ISMIP+ F D SITH+ALGSNPL CDC+LKWLS+W+K Y+EPGIA+C+ P+ + + L+L+TP S F C+ + EI++KCD C++ PC+N A C+ ++ C C PGY+G CE IDACYGNPC + GTCTV E GR+ C C GYSG RCE ID+C ++ C NNATC D V + C C G++GE C AKI+ C + C NG C+ + + Y+C CVPG+ G +CS ++DDC +HMCQNGGTC+DG Y C C EF GK+CE P+++ +YPQ+SPCQQHECK G CF P S DY+CKC PGY+GK CEYLTSL+F ++ ++EPL TKP AN+T+ + + +GVL+Y G N+HLAVELF GRIRVSYDVGNYPVSTM+S+E ++DG H +++LA KN TLRVD G +RSI+NEG+
Sbjct: 292 LTENAPMECGGRSLCPHPCRCADGIVDCREKSLTSVPSTLPEDTTELRLEQNYITEIPPKAFANHRRLKRIDLSNNNISRVAYDAFSGLKSLTSLVLYGNKIKDLPASVFKGLTSLQLLLLNANEITCVRRDAFKDLQNLSLLSLYDNNIQTLANGTFDSLKSIQTLHLARNPFICDCNLRWLGDYLHQNPIETSGARCDSPKRMQRRRIEALKDEKFKCTEDHSKIKYAGECRMDQECPAACHCDRTTVDCSARGLKEIPRDIPLYTTELILNDNELTRVRSDGLFGRLPNLVKLDLRRNQIANIEPNAFEGATKIQELFMSENKIAEVHNKMFLGLHQLKTLSLYDNLISCVMPGSFDFLSSLTQLNLASNPFRCNCHLGWFSDWLRKKQLGGPPARCASPAKVRDVPVKDLPHFEFKCTSEADQGCLGEGYCPPSCTCTGTVVRCSRNKLKEIPKSIPSETTELYLESNDISMVHTDRISHLKSLTRLDLSNNKIGILSNHTFVNLSRLSTLIISYNNLQCIQQYALAGLKQLKVLSLHGNHISMIPDGTFADLQSITHIALGSNPLYCDCSLKWLSEWVKRDYVEPGIARCAEPELMKDKLILSTPASQFLCKGK-VSNEILSKCDACYTFPCKNNAVCKPLPERQYECQCPPGYHGAHCEFMIDACYGNPCRNNGTCTVLEEGRFSCQCTSGYSGARCEINIDDCTNHKCLNNATCVDGVNSYSCACIPGFTGEFCDAKIEFCGKDFNPCQNGAKCVDHSTH-YSCECVPGYRGLNCSENIDDCVNHMCQNGGTCIDGINDYVCKCPSEFTGKFCEGTPMVAMLYPQTSPCQQHECKFGVCFQPNPSSADYICKCGPGYSGKRCEYLTSLTFLHNNSFVELEPLRTKPEANVTIVFSSTQQNGVLMYDGNNDHLAVELFNGRIRVSYDVGNYPVSTMYSFEMVADGKYHMVELLAIKKNFTLRVDRGLARSIINEGS 1271 HSP 2 Score: 333.183 bits (853), Expect = 3.312e-90 Identity = 202/661 (30.56%), Postives = 319/661 (48.26%), Query Frame = 0
Query: 147 VNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLR------------------------RIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSAS---CNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDL------------------------SHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEG-------CLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAE 748
+ CP+ C C VDC + L + P IP L L+ NNI I F+ L LR R+ L++N ++ IPD+ + L L L N + I +F SL+ L L++N+I C+ +A L L +L+L +NNI L F+G+ L+ L L +N FAC C L+WL +L+ P RC +P +L+ + ++ LH C L+ + C S CP PC C++G+VDC + + +PS +P+ T EL+L N + +P N L ++LS N IS + +F G +S+ L L + N +T +R + L L+ L L++NNI ++ G FD L + ++ NP CDC++ W GD+L ++ A C +P ++ R+++ L F+C +ED C CP C C T + CS LK +P ++P +EL L+ N++ + F L L +LD+ N+I+ I+P F T++ L +S N+I + F GLH L+ LSL+ N IS + +FD +S+T L L SNP C+C+L W S W++ + + A+C+ P + + V P FKC +E
Sbjct: 58 MRCPRLCSCTGQTVDCSHRGLTQVPRKIPLDTDRLDLQGNNITVIYESDFQSLSKLRILQLTDNQIYTIEKDALIDLISLERLRLNSNRLKSIPDNFLSSAANLLRLDLSHNALSAIPKRAFKGATSLRSLQLDNNQITCLDEHAIKGLAELEILTLNNNNITALPRDMFSGMPRLRALRLSENPFACDCHLSWLARYLKNAPRLAPYTRCHSPSQLKGQNVADLHEQEFKCSGLTENAPMECGGRSLCPHPCRCADGIVDCREKSLTSVPSTLPEDTTELRLEQNYITEIPPKAFANHR-RLKRIDLSNNNISRVAYDAFSGLKSLTSLVLYGNKIKDLPASVFKGLTSLQLLLLNANEITCVRRDAFKDLQNLSLLSLYDNNIQTLANGTFDSLKSIQTLHLARNPFICDCNLRWLGDYLHQNPIETSGARCDSPKRMQRRRIEALKDEKFKC--TEDHSKIKYAGECRMDQECPAACHCDRTTVDCSARGLKEIPRDIPLYTTELILNDNELTRVRSDGLFGRLPNLVKLDLRRNQIANIEPNAFEGATKIQELFMSENKIAEVHNKMFLGLHQLKTLSLYDNLISCVMPGSFDFLSSLTQLNLASNPFRCNCHLGWFSDWLRKKQLGGPPARCASPAKVRDVPVKDLPHFEFKCTSE 715
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Match: gi|1121162481|ref|XP_019526801.1| (PREDICTED: LOW QUALITY PROTEIN: protein slit-like [Aedes albopictus]) HSP 1 Score: 1054.66 bits (2726), Expect = 0.000e+0 Identity = 515/982 (52.44%), Postives = 670/982 (68.23%), Query Frame = 0
Query: 135 LTAGESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSS-----ASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPK-DSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEGA 1110
LT + R CP C C DGIVDCR+K L PA++P TELRLEQN I IPPKAF + L+RIDLSNN I + D F+GL LTSLVLYGN+I+ + S F L SLQLLLLN+N+I C+ +AF L+ L LLSLYDNNIQTL NGTF+ L S+QTLHL +N F C C+L WL D+L +NP+ETSGARC++P ++QR++I +L + C + S C CP C C VDCS RG+K IP DIP T EL L+DN L V S GLF LP L L+L RN I+ IE +FEGA I L +S N L + ++ GL L TL L++N I C+ PG+FD L L+ N+ SNP +C+CH+ WF DWLR + A CA+PA +RD +++LP F+C D+GCL + YCP C CTGTV+RCS +LK +P ++P E +ELYL+ N+I + SHL+ L+RLD+SNNKI ++ Y FANL+RLSTLIISYN ++CIQ +A +GL L++LSLHGN ISMIP+ F D SITH+ALGSNPL CDC+LKWLS+W+K Y+EPGIA+C+ P+ + + L+L+TP S F C+ + EI++KCD C++ PC+N A C+ ++ C C PGY+G CE IDACYGNPC + GTCTV E GR+ C C GYSG RCE ID+C ++ C NN TC D V + C C G++GE C +KI+ C + C NG C+ + + Y+C C PG+ G +CS ++DDC +HMCQNGGTC+DG Y C C EF GK+CE P+++ +YPQ+SPCQ HECK+G CF P S DYVCKC PGY+GK CEYLTSL+F ++ ++EPL TKP AN+T+ + + +GVL+Y G N+HLAVELF GRIRVSYDVGNYPVSTM+S+E ++DG H +++LA KN TLRVD G +RSI+NEG+
Sbjct: 297 LTENAPMECGGRSLCPHPCRCADGIVDCREKSLTSVPATLPEDTTELRLEQNYITEIPPKAFANHRRLKRIDLSNNNISRVAYDAFSGLKSLTSLVLYGNKIKDLPASVFKGLTSLQLLLLNANEITCVRRDAFKDLQNLSLLSLYDNNIQTLANGTFDSLKSIQTLHLARNPFICDCNLRWLGDYLHQNPIETSGARCDSPKRMQRRRIEALKDEKFKCTEDHSKIKYAGECRMDQECPAACHCDRTTVDCSARGLKEIPRDIPLYTTELILNDNELTRVRSDGLFGRLPNLVKLDLRRNQITNIEANAFEGATKIQELFMSENKLAEVHNKMFLGLHQLKTLSLYDNLISCVMPGSFDFLSSLTQLNLASNPFRCNCHLGWFSDWLRKKQLGGPPARCASPAKVRDVPVKDLPHFEFKCTSEADQGCLGEGYCPPSCTCTGTVVRCSRNKLKEIPKSIPSETTELYLESNEISMVHTDRISHLKSLTRLDLSNNKIGILSNYTFANLSRLSTLIISYNNLQCIQQYALAGLKQLKVLSLHGNHISMIPDGTFSDLQSITHIALGSNPLYCDCSLKWLSEWVKRDYVEPGIARCAEPEMMKDKLILSTPASQFLCKGK-VSNEILSKCDACYTFPCKNDAVCKPLPERQYECQCPPGYHGAHCEFMIDACYGNPCRNNGTCTVLEEGRFSCQCMSGYSGARCEINIDDCTNHKCLNNGTCVDGVNSYSCACIPGFTGEFCDSKIEFCGKDFNPCQNGAKCVDRSTH-YSCECTPGYRGLNCSENIDDCVNHMCQNGGTCIDGINDYVCKCPSEFTGKFCEGTPMVAMLYPQTSPCQHHECKYGVCFQPNPASADYVCKCGPGYSGKRCEYLTSLTFLHNNSFVELEPLRTKPEANVTIVFSSTQQNGVLMYDGNNDHLAVELFNGRIRVSYDVGNYPVSTMYSFEMVADGKYHMVELLAIKKNFTLRVDRGLARSIINEGS 1276 HSP 2 Score: 328.946 bits (842), Expect = 7.563e-89 Identity = 200/665 (30.08%), Postives = 319/665 (47.97%), Query Frame = 0
Query: 143 LDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLR------------------------RIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSAS---CNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDL------------------------SHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEG-------CLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAE 748
L+ + CP+ C C VDC + L + P IP L L+ NNI I F L LR R+ L++N ++ IPD+ + L L L N + I +F SL+ L L++N+I C+ +A L L +L+L +NNI L F+G+ L+ L L +N FAC C L+WL +L+ P RC +P +L+ + ++ LH C L+ + C S CP PC C++G+VDC + + +P+ +P+ T EL+L N + +P N L ++LS N IS + +F G +S+ L L + N +T +R + L L+ L L++NNI ++ G FD L + ++ NP CDC++ W GD+L ++ A C +P ++ R+++ L F+C +ED C CP C C T + CS LK +P ++P +EL L+ N++ + F L L +LD+ N+I+ I+ F T++ L +S N++ + F GLH L+ LSL+ N IS + +FD +S+T L L SNP C+C+L W S W++ + + A+C+ P + + V P FKC +E
Sbjct: 59 LNGEMRCPRLCSCTGQTVDCSHRGLTQVPRKIPLDTDRLDLQGNNITVIYESDFLSLSKLRILQLTDNQIYTIEKDALIDLISLERLRLNSNRLKSIPDNFLSSAANLLRLDLSHNALSAIPKRAFKGATSLRSLQLDNNQITCLDEHAIKGLAELEILTLNNNNITALPRDMFSGMPRLRALRLSENPFACDCHLSWLARYLKNAPRLAPYTRCHSPSQLKGQNVADLHEQEFKCSGLTENAPMECGGRSLCPHPCRCADGIVDCREKSLTSVPATLPEDTTELRLEQNYITEIPPKAFANHR-RLKRIDLSNNNISRVAYDAFSGLKSLTSLVLYGNKIKDLPASVFKGLTSLQLLLLNANEITCVRRDAFKDLQNLSLLSLYDNNIQTLANGTFDSLKSIQTLHLARNPFICDCNLRWLGDYLHQNPIETSGARCDSPKRMQRRRIEALKDEKFKC--TEDHSKIKYAGECRMDQECPAACHCDRTTVDCSARGLKEIPRDIPLYTTELILNDNELTRVRSDGLFGRLPNLVKLDLRRNQITNIEANAFEGATKIQELFMSENKLAEVHNKMFLGLHQLKTLSLYDNLISCVMPGSFDFLSSLTQLNLASNPFRCNCHLGWFSDWLRKKQLGGPPARCASPAKVRDVPVKDLPHFEFKCTSE 720
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Match: gi|108874764|gb|EAT38989.1| (AAEL009175-PA, partial [Aedes aegypti]) HSP 1 Score: 1051.58 bits (2718), Expect = 0.000e+0 Identity = 516/982 (52.55%), Postives = 672/982 (68.43%), Query Frame = 0
Query: 135 LTAGESICLDSRVNCPKECHCNDGIVDCRDKKLERFPASIPHGATELRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSS-----ASCNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDLSHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEGCLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEMAHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAEDTPQEIVAKCDPCHSSPCQNKATCRAHHGLEFICDCVPGYYGTVCEKNIDACYGNPCSHGGTCTVPENGRYVCDCPPGYSGLRCEFEIDECESNPCQNNATCFDEVARFRCDCPEGYSGETCSAKIDPCMNATDKCLNGGSCLPSAVNTYTCSCVPGFSGPDCSVSLDDCRSHMCQNGGTCLDGNGTYSCACTPEFAGKYCELEPIISHMYPQSSPCQQHECKHGTCFMPK-DSKDYVCKCDPGYTGKYCEYLTSLSFDQKGAYAQMEPLNTKPLANITMEVVTLETSGVLVYVGRNEHLAVELFKGRIRVSYDVGNYPVSTMFSYEKISDGLPHRIQILAQGKNLTLRVDDGQSRSIVNEGA 1110
LT + R CP C C DGIVDCR+K L P+++P TELRLEQN I IPPKAF + L+RIDLSNN I + D F+GL LTSLVLYGN+I+ + S F L SLQLLLLN+N+I C+ +AF L+ L LLSLYDNNIQTL NGTF+ L S+QTLHL +N F C C+L WL D+L +NP+ETSGARC++P ++QR++I +L + C + S C CP C C VDCS RG+K IP DIP T EL L+DN L V S GLF LP L L+L RN I+ IE +FEGA I L +S N + + ++ GL L TL L++N I C+ PG+FD L L+ N+ SNP +C+CH+ WF DWLR + A CA+PA +RD +++LP F+C D+GCL + YCP C CTGTV+RCS +LK +P ++P E +ELYL+ NDI + SHL+ L+RLD+SNNKI ++ + FANL+RLSTLIISYN ++CIQ +A +GL L++LSLHGN ISMIP+ F D SITH+ALGSNPL CDC+LKWLS+W+K Y+EPGIA+C+ P+ + + L+L+TP S F C+ + EI++KCD C++ PC+N A C+ ++ C C PGY+G CE IDACYGNPC + GTCTV E GR+ C C GYSG RCE ID+C ++ C NNATC D V + C C G++GE C AKI+ C + C NG C+ + + Y+C CVPG+ G +CS ++DDC +HMCQNGGTC+DG Y C C EF GK+CE P+++ +YPQ+SPCQQHECK G CF P S DY+CKC PGY+GK CEYLTSL+F ++ ++EPL TKP AN+T+ + + +GVL+Y G N+HLAVELF GRIRVSYDVGNYPVSTM+S+E ++DG H +++LA KN TLRVD G +RSI+NEG+
Sbjct: 200 LTENAPMECGGRSLCPHPCRCADGIVDCREKSLTSVPSTLPEDTTELRLEQNYITEIPPKAFANHRRLKRIDLSNNNISRVAYDAFSGLKSLTSLVLYGNKIKDLPASVFKGLTSLQLLLLNANEITCVRRDAFKDLQNLSLLSLYDNNIQTLANGTFDSLKSIQTLHLARNPFICDCNLRWLGDYLHQNPIETSGARCDSPKRMQRRRIEALKDEKFKCTEDHSKIKYAGECRMDQECPAACHCDRTTVDCSARGLKEIPRDIPLYTTELILNDNELTRVRSDGLFGRLPNLVKLDLRRNQIANIEPNAFEGATKIQELFMSENKIAEVHNKMFLGLHQLKTLSLYDNLISCVMPGSFDFLSSLTQLNLASNPFRCNCHLGWFSDWLRKKQLGGPPARCASPAKVRDVPVKDLPHFEFKCTSEADQGCLGEGYCPPSCTCTGTVVRCSRNKLKEIPKSIPSETTELYLESNDISMVHTDRISHLKSLTRLDLSNNKIGILSNHTFANLSRLSTLIISYNNLQCIQQYALAGLKQLKVLSLHGNHISMIPDGTFADLQSITHIALGSNPLYCDCSLKWLSEWVKRDYVEPGIARCAEPELMKDKLILSTPASQFLCKGK-VSNEILSKCDACYTFPCKNNAVCKPLPERQYECQCPPGYHGAHCEFMIDACYGNPCRNNGTCTVLEEGRFSCQCTSGYSGARCEINIDDCTNHKCLNNATCVDGVNSYSCACIPGFTGEFCDAKIEFCGKDFNPCQNGAKCVDHSTH-YSCECVPGYRGLNCSENIDDCVNHMCQNGGTCIDGINDYICKCPSEFTGKFCEGTPMVAMLYPQTSPCQQHECKFGVCFQPNPSSADYICKCGPGYSGKRCEYLTSLTFLHNNSFVELEPLRTKPEANVTIVFSSTQQNGVLMYDGNNDHLAVELFNGRIRVSYDVGNYPVSTMYSFEMVADGKYHMVELLAIKKNFTLRVDRGLARSIINEGS 1179 HSP 2 Score: 320.087 bits (819), Expect = 1.192e-86 Identity = 189/603 (31.34%), Postives = 302/603 (50.08%), Query Frame = 0
Query: 181 LRLEQNNIVSIPPKAFKKLKWLRRIDLSNNEIRLIPDDTFTGLPRLTSLVLYGNQIERISGSSFYDLPSLQLLLLNSNKIRCIHPNAFNRLEVLHLLSLYDNNIQTLDNGTFNGLSSLQTLHLGKNAFACGCDLAWLTDWLQKNPVETSGARCETPVKLQRKKISSLHLGRSNCLDLSSAS---CNWTSPCPFPCSCSEGVVDCSRRGVKIIPSDIPKTTKELQLSDNVLDVVPSVGLFNLLPALSTLNLSRNFISVIEEGSFEGARSIHILDL------------------------SHNSLTTIRGRLLRGLDGLTTLHLHNNNIHCISPGAFDLLPKLSFTNILSNPIQCDCHMAWFGDWLRSQNNTSALATCATPAHLRDRQLQELPLPSFRCKGSEDEG-------CLEQHYCPRECVCTGTVIRCSHAQLKTVPSNVPKEASELYLDVNDIDDLEM-AHFSHLQRLSRLDISNNKISVIKPYVFANLTRLSTLIISYNEIKCIQPHAFSGLHHLRILSLHGNSISMIPNSAFDDHNSITHLALGSNPLLCDCNLKWLSQWMKNQYIEPGIAKCSYPKSLANHLVLTTPESSFKCQAE 748
L+L N I +I A L L R+ L++N ++ IPD+ + L L L N + I +F SL+ L L++N+I C+ +A L L +L+L +NNI L F+G+ L+ L L +N FAC C L+WL +L+ P RC +P +L+ + ++ LH C L+ + C S CP PC C++G+VDC + + +PS +P+ T EL+L N + +P N L ++LS N IS + +F G +S+ L L + N +T +R + L L+ L L++NNI ++ G FD L + ++ NP CDC++ W GD+L ++ A C +P ++ R+++ L F+C +ED C CP C C T + CS LK +P ++P +EL L+ N++ + F L L +LD+ N+I+ I+P F T++ L +S N+I + F GLH L+ LSL+ N IS + +FD +S+T L L SNP C+C+L W S W++ + + A+C+ P + + V P FKC +E
Sbjct: 24 LQLTDNQIYTIEKDALIDLISLERLRLNSNRLKSIPDNFLSSAANLLRLDLSHNALSAIPKRAFKGATSLRSLQLDNNQITCLDEHAIKGLAELEILTLNNNNITALPRDMFSGMPRLRALRLSENPFACDCHLSWLARYLKNAPRLAPYTRCHSPSQLKGQNVADLHEQEFKCSGLTENAPMECGGRSLCPHPCRCADGIVDCREKSLTSVPSTLPEDTTELRLEQNYITEIPPKAFANHR-RLKRIDLSNNNISRVAYDAFSGLKSLTSLVLYGNKIKDLPASVFKGLTSLQLLLLNANEITCVRRDAFKDLQNLSLLSLYDNNIQTLANGTFDSLKSIQTLHLARNPFICDCNLRWLGDYLHQNPIETSGARCDSPKRMQRRRIEALKDEKFKC--TEDHSKIKYAGECRMDQECPAACHCDRTTVDCSARGLKEIPRDIPLYTTELILNDNELTRVRSDGLFGRLPNLVKLDLRRNQIANIEPNAFEGATKIQELFMSENKIAEVHNKMFLGLHQLKTLSLYDNLISCVMPGSFDFLSSLTQLNLASNPFRCNCHLGWFSDWLRKKQLGGPPARCASPAKVRDVPVKDLPHFEFKCTSE 623 The following BLAST results are available for this feature:
BLAST of hypothetical protein DAPPUDRAFT_304927 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 25
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BLAST of hypothetical protein DAPPUDRAFT_304927 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of hypothetical protein DAPPUDRAFT_304927 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold483_size159862:65074..71683- Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold483_size159862-snap-gene-0.41 ID=maker-scaffold483_size159862-snap-gene-0.41|Name=hypothetical protein DAPPUDRAFT_304927|organism=Tigriopus kingsejongensis|type=gene|length=6610bp|location=Sequence derived from alignment at scaffold483_size159862:65074..71683- (Tigriopus kingsejongensis)back to top Synonyms
The feature 'hypothetical protein DAPPUDRAFT_304927' has the following synonyms
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