BLAST of amp dependent coa ligase vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 154.451 bits (389), Expect = 5.676e-41
Identity = 119/479 (24.84%), Postives = 228/479 (47.60%), Query Frame = 0

Query:   63 FLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWAITESESSMGPDLVDVN-NDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGMLVFNLVPFSLQSGFFFA------LDALKRSQMSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSS-KNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETG-LFMSAPTCKHLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTP-QFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILIT-HPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
            F++   KF  +    GL++GD +H+ +G    +  P+   +   G  +S+   ++  +T+ +++Q    K++ C   T          +  +     +I +D  +     I  S  +  P+ ++ +   LA I ++SGTTG+PKGI     S WN    S   P T   +     F+  SGF ++       D      +S  +  +  +  II    +     +++ A  +V +  K+     + +    ++K I PAGA     + D +R+L  NLE + N++   E+G L + +   +  G +   ++  +  I +G+RLGP  +GEL  + P +    YLN P+ ++  F  +G+   GDM +Y+  G +FY  R+KE+LK    HV P E+E+I+ + +  + +  +F K ++++  ++   +V+     +      +E+  FVN++++D+K +   I+    L RN  GKL R
Sbjct:   79 FIEKVPKFAKYLYENGLRKGDVVHIIIGNRGDVYIPVHATLLCQG-VVSISVDNISTETLLSEVQETQAKFVICTNRT---------YYKVKDLSSIIINLDTQLHAILEIDMSTQTQIPEQIEFDLQTLAVILWTSGTTGKPKGIQLNMVSLWNLLFLS---PKTDGNMLITCNFAHVSGFNYSCFWSINFDVCTFHFISPEDVTSLGVEKIIFTLSQIPFTTIVVSASHIVYIAEKIVNDQIEYSSNFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVGNMEVYIRDIHSGERLGPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSK-TEKIQTILGGIVVIK----KGYRIDPEEIRQFVNDRIDDYKHLEIIILTNEPLPRNPNGKLLR 539          

BLAST of amp dependent coa ligase vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 140.969 bits (354), Expect = 3.925e-36
Identity = 131/496 (26.41%), Postives = 227/496 (45.77%), Query Frame = 0

Query:   67 SHKFNDFFRHKGLKRGDKIHLFLG--PYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFG----------PESPKPEVIIMDVDMVENWAITESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGMLVFNLVP--FSLQSGFFFALDALKRSQMSVNNFGADTMSAIIEGCPKWKPIALI------IPAFLMV--PLNHKLKQSSKNTERLVDLKFIVPAGAAI--PDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAP-------TCKHLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDE-LTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
            S KF       G K+GD + L +   P   IAF   MG    G  ++  +P+ + + IA QL N+  KY+  +   +     +  ++            E+P      M++ + ++ +  +   ++ P     + D+ A+ YSSGTTG PKG+  TH++  N   +    P+    + + V    S+    FF + A+  S  +   FG   ++      PK++P   I       P  ++V  PL   +  S      L+    ++  GA+   P  +E  + +   +L   E    T  + +    P       +C   G + P    K++ IDTGK LGPGE GELCV  PQ M+ Y    + +  +  + G+ H GD+ YY+ +G+ + + RLKE++K +   VAP+ELE ++  +P V D    G P +   +L  A +V        N S++++ +  FV E+V   K++   ++F+  L +N  GK+ R
Sbjct:   97 SQKFGSALLRLGAKKGDVMGLVVPNIPEFVIAF---MGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQKIIVIGMEETPSDCKSFMEMAIFDDGSFFDQRETIDP-----HKDILALPYSSGTTGPPKGVALTHSNLVNNI-TQLSHPSIHNFIGDEVEQHISIAVLPFFHIFAMTCSLSAGLRFGVKLIT-----LPKFEPEMFINSLVQYKPTHMLVVPPLVSFMTSSPLIKPSLLSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQEGYGMTECSPVTHVQPKDGIIYGSC---GNLIPNTIGKIIDIDTGKVLGPGENGELCVSGPQIMQGYYKNKKATDKIIQN-GWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVV------RKNRSVMEQGIIDFVTEKVAPHKKLGAGVMFVDTLPKNQTGKILR 568          

BLAST of amp dependent coa ligase vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 114.39 bits (285), Expect = 3.188e-27
Identity = 123/498 (24.70%), Postives = 218/498 (43.78%), Query Frame = 0

Query:   59 SPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQL----QNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWAITESESSMGPDLVDVNNDLAAIFYSSGTTG---QPKGICHTHTSFWNWTGSSTDIP-TTGMLVFNLVPFSLQ----------SGFFFALDAL---KRSQM---SVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAPTCKHLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPA-VKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDEL-TGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
            SP +F+K + +F  F   +         L L        P+ +G W+ G  +SL  PS   +TI  QL    +  +++ I  + +T  +              P++  + VD + +        S+    ++ +N++  IF   GTT    +  G+ ++  +  N     + +P     ++ N  P SL+           GF   L  +   KR+ +   S N +   + + +       KP  +I      V +N      S   + L  +  I P G +I  + E   R   P+L+ I   +G+ ET       + +HLG + PK Q ++++ + G+ LGP   G++ VK    M+ Y+   E +      +GF ++GD+ YYD  G+IF+  R K+++K    HV P E+ES + +  + VKD  VFG+      + +S  IVL+    E   SL + L   F   +   +K ++G +   S + RN+ GK+ R
Sbjct:   71 SPREFVKYTKRFAHFISERHGNARFATVLLLNNTRFFFIPV-VGTWFLGSSVSLFQPSSSRETIERQLMILKEELNLEEIVVITETFAI--------------PKISNIGVDRIISLTPVPGYMSLQEIFMNDDNEVVEIFNREGTTSLQSEIAGVLYSAENILNSIHYCSYVPFQIHHVIKNYEPSSLRLMILTNFFHPGGFTEGLRNILPQKRTGIILRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFD-LSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTETLSISLGISDQHLGRLFPKYQLRILNPN-GEPLGPNSVGDIVVKPGYGMRRYIGDHEYN---ITQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDKNGIERLSGVIVLH--HIEKGLSLQNVLERTFEAYKFPRYKWLQGGVRVTSAIPRNANGKIFR 546          

BLAST of amp dependent coa ligase vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 109.768 bits (273), Expect = 1.380e-25
Identity = 116/498 (23.29%), Postives = 217/498 (43.57%), Query Frame = 0

Query:   78 GLKRGDKIHLFLGPYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICC----VQDTVPLAHSA-----LHLFGPESPKPEVIIMDVDMVENWAITESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTS----FWNWTGSST--------DIPTTGMLVFNLVPF-SLQSGFFFALDALKRSQMSVNNFG---ADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLF-MSAPTCKH------------------LGWINPKVQAKVVHIDTGKRL-GPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
            G  +GD + L+   +    + +A+G+W  GG +   +  L    +  Q++ +  K + C      D +    +      L + G E    + +I+  D++ +    + +      + D   D+  + ++S ++G  KGI HT+ S    F++  G++         D        F++  F +   G  + +     S+ S  NF     D  +A I  C  W+     I      PL      SS        LK ++     +  ++   V   +P+++ +   +G  E GL  ++ P  K                   +G  N     K++    G  + GP + GE+CVK+PQ M  YLN  E S   FD++ + H GD+ YYD++G +++++++  ++++ +  VAP+ LES L+    + D  V G P+ E   +  A +VL    FE  E   + LT  +  +++D +RIRG + F+  + R+   K+ R
Sbjct:  108 GFVKGDVLTLYCSNFVEY-WILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVCDDFNYVDGLDFLENTNMIEKLIIIGNECTAQDKVILMTDLLND----DGKKVPQKVIFDWAKDVIYMPFTSTSSG-AKGILHTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYGISIYTLSEFSEQNFLEALVDXKTASI-SCYAWQ-----IRILSQSPLVSNYDLSS--------LKVLMTGCGLLSSTIRMEVLDRIPSIKYVREAYGLNECGLVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYLNKEESSA--FDNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKP-GFEETE---ENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLR 579          

BLAST of amp dependent coa ligase vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 93.5893 bits (231), Expect = 1.689e-20
Identity = 62/211 (29.38%), Postives = 110/211 (52.13%), Query Frame = 0

Query:  335 IENIFGTGETGLFMSAPTCKHL-GWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKE-YLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESIL-ITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSE-LERNSFGKLQRLQTFGIIQSLL 541
            + N +G+ E+G      T +H  G +  + +  +  ++TG+ LGP + GEL  +        YLN  E ++ +F+ +G+ H GD  +Y  +G  F+  R+ E++K +  HV P ELE +L   H  + +  V     ++   ++ A IV+  D     E++  EL    N++V+++KRI G ++   E L RN+ GKL R +   + +S L
Sbjct:  286 VPNFYGSTESGGGFLVSTNQHSNGGVVARTETYIRDLNTGQHLGPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVV-PVVEKKQTVLGAIIVVKNDKKINVETIRKEL----NKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEIQKLFKSSL 491          

HSP 2 Score: 59.3066 bits (142), Expect = 1.523e-9
Identity = 62/261 (23.75%), Postives = 108/261 (41.38%), Query Frame = 0

Query:    4 NRVTQDYLIEGKDQSSWELAIPNGSIYVE--LDRIVRQRNLKTNNKWLFDYTSDTHYSPSQFLKD---SHKFNDFFR----------HKGLKRGDKIHLFLGPYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWAITESESSMGP--DLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGMLVFNLVPFSLQSGFFFAL 247
            +++++DY++  K   +  L         E  LD+I +   +  + + L +      + P + + D   +H   DF             KGL++ D +HL +G    I  P+   I   G  IS+   S+   T+  +L +   + + C   T     +             +I MD D+ E  A   +   + P  D+  +  D+  I +SSGTTGQPKG+  T  SF +   ++  +    +L  N   F   SGF  +L
Sbjct:    7 SKISEDYVLTTKSDDNRTLQAEYNQNLAECFLDKITKYLEVNEDKELLVN--CGKRFFPDETVSDGPVAHTLKDFLHKVPALAKSLYEKGLRKSDVVHLIIGNRADIYLPVFATILCQG-VISISVDSISHDTLMTELNDTQAEIVFCTNKTFKKVKNC---------GCTIINMDTDIEEMIANGCTSKVIVPKQDVFSLK-DVFVILWSSGTTGQPKGVQMTVVSFLDSMFATPKVKGNMLLSAN---FGHISGFISSL 251          

BLAST of amp dependent coa ligase vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 68.5514 bits (166), Expect = 2.918e-12
Identity = 58/225 (25.78%), Postives = 101/225 (44.89%), Query Frame = 0

Query:  322 EDSVRQLLPNLEGIENIFGTGETG--LFMSAPTCKHLGWINP------KVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISA--CIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQTFGI 536
            ED  + L  N++ + NI+G  ETG   F+ +P       IN        V+AK+V    GK L     GEL V+TP  M  Y    + SQ     + + H GD+     +G    + RL +++    +++ P E+E  L+ HP + +  + G P D + + + A  C+      F+  E    ++  F         +I    +F+ +  +   GK+++L+  GI
Sbjct:  699 EDFEKHL--NMKSLMNIYGATETGPATFVPSPEDSFEVRINTVGPPLGHVEAKIVD-QNGKILPVNTPGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEELCAWICLEEGAKEFDLKEHCKGKIAHF---------KIPKYSMFVKDFPKTLSGKVKKLEMRGI 911          

HSP 2 Score: 65.0846 bits (157), Expect = 2.998e-11
Identity = 52/214 (24.30%), Postives = 100/214 (46.73%), Query Frame = 0

Query:  331 NLEGIENIFGTGETG--LFMSAPTCKHLGWINP------KVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQTFGI 536
            N++ + NI+G  ETG   F++ P       IN        ++AK+V    GK L     GEL V+TP  M  Y    + S+ +   + + H GD+     +G    + RL +++    +++ P E+E +L+ HP++ +  + G P + + + + A I L   + E       +L      ++  FK  + P +F+ +  +   GK+++ +   I
Sbjct:  335 NMKSLMNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVD-QNGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEELCAWICLEEGAKEF------DLKEHCKGKISHFKIPKYP-MFVKDFPKTLSGKVKKFEMKAI 540          

HSP 3 Score: 63.5438 bits (153), Expect = 1.037e-10
Identity = 52/210 (24.76%), Postives = 96/210 (45.71%), Query Frame = 0

Query:  335 IENIFGTGETG--LFMSAPTCKHLGWINP------KVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQTFGI 536
            + NI+G  ETG   F++ P       IN        ++AK+V    GK L     GEL V+TP  M  Y    + S+ +   + + H GD+     +G    + RL +++    +++ P E+E  L+ HP++ +  + G P D + + + A I L   + E       +L      ++  FK  + P +F+ +  +   GK+++ +   I
Sbjct:  111 LRNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVD-QNGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEELCAWICLEEGAKEF------DLKEHCKGKIAHFKIPKYP-MFVKDFPKTLSGKVKKFEMKAI 312          

BLAST of amp dependent coa ligase vs. SwissProt
Match: gi|1168218|sp|P41636.1|4CL_PINTA (RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName: Full=4-coumaroyl-CoA synthase)

HSP 1 Score: 160.614 bits (405), Expect = 1.033e-41
Identity = 147/517 (28.43%), Postives = 241/517 (46.62%), Query Frame = 0

Query:   44 TNNKWLFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENW----AITESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGM------LVFNLVP----FSLQSGFFFALDALKRSQMSVNNFGADTMSAIIEGCPKWK-PIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAA-IPDSLEDSVRQLLPNLEGIENIFGTG----ETG--LFMSAPTCKH--------LGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
             +   L D  +D  Y  S+    S K        GL++G  + L L      AF + MG    G  ++  +P  K   IA Q + A  + I      V LA     L   +S    VI +D    E       +TE++ +  P +    +D+ A+ YSSGTTG PKG+  TH    +      D     +      ++  ++P    +SL S    AL A   + + +  F   T   +I+   K+K  +A I+P     P+   + +S   ++  V    I+ +GAA +   LED++R+  P     + IFG G    E G  L M+    K+         G +    Q K++  +TG+ L   +AGE+C++ P+ MK Y+N PE + A  D EG+ H GD+ Y D +  IF + R+KEI+KY+   VAP ELE++L+ HP++ D  V  +  +E  ++  A +V +++  E       E+  FV +QV  +K+I   + F+  + ++  GK+ R
Sbjct:   39 ADRPCLIDGATDRTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAF-VFMGASVRGAIVTTANPFYKPGEIAKQAKAAGARII------VTLAAYVEKLADLQSHDVLVITIDDAPKEGCQHISVLTEADETQCPAVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFHSDDVILCVLPLFHIYSLNSVLLCALRA-GAATLIMQKFNLTTCLELIQ---KYKVTVAPIVP-----PIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFP-----KAIFGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVEYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEEAGEVPVAFVVKSSEISE------QEIKEFVAKQVIFYKKIHR-VYFVDAIPKSPSGKILR 527          

BLAST of amp dependent coa ligase vs. SwissProt
Match: gi|126500|sp|P13129.1|LUCI_LUCCR (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 159.844 bits (403), Expect = 2.038e-41
Identity = 117/357 (32.77%), Postives = 173/357 (48.46%), Query Frame = 0

Query:  185 VDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTD-----IPTTGMLVFNLVPFSLQSGFFFALDALK---RSQMSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGET-GLFMSAPTCKHL----GWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKL 528
            VD    +A I  SSG+TG PKG+  TH +       + D       + G  V  +VPF    G F  L  L    R  M +  F  +T    ++    +K  ++I+   L   LN   K    N   L +L  I   GA +   + ++V +   NL G+   +G  ET    +  P         G + P  +AKV+ +DT K LGP   GE+CVK P  MK Y+N PE ++ L D EG+ H GD+ YYD E   F + RLK ++KY+   V P ELES+L+ HP++ D  V G P     +L  A +VL +      + ++D    +V  QV + KR+RG + F+ E+ +   GK+
Sbjct:  188 VDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLICGFRVVM-LTKFDEETFLKTLQ---DYKCTSVILVPTLFAILN---KSELLNKYDLSNLVEIASGGAPLSKEVGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMD----YVASQVSNAKRLRGGVRFVDEVPKGLTGKI 532          

BLAST of amp dependent coa ligase vs. SwissProt
Match: gi|126501|sp|P08659.1|LUCI_PHOPY (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 157.532 bits (397), Expect = 1.213e-40
Identity = 118/367 (32.15%), Postives = 176/367 (47.96%), Query Frame = 0

Query:  176 SESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTD-------IPTTGMLVFNLVPFSLQSGFFFALDALKRSQMSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTER--LVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGET-GLFMSAPTCKH----LGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKL 528
            +E    P+  D +  +A I  SSG+TG PKG+   H +       + D       IP T +L  ++VPF    G F  L  L      +  F    M    E           I + L+VP        S   ++  L +L  I   GA +   + ++V +   +L GI   +G  ET    +  P        +G + P  +AKVV +DTGK LG  + GELCV+ P  M  Y+N PE + AL D +G+ H GD+ Y+D +   F + RLK ++KY+   VAP ELESIL+ HP + D  V G P D+  +L +A +VL        + +VD    +V  QV   K++RG ++F+ E+ +   GKL
Sbjct:  177 NEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAIL--SVVPFHHGFGMFTTLGYL------ICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRF-HLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVD----YVASQVTTAKKLRGGVVFVDEVPKGLTGKL 530          

BLAST of amp dependent coa ligase vs. SwissProt
Match: gi|12229666|sp|Q9S777.1|4CL3_ARATH (RecName: Full=4-coumarate--CoA ligase 3; Short=4CL 3; AltName: Full=4-coumarate--CoA ligase isoform 3; Short=At4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 157.147 bits (396), Expect = 1.826e-40
Identity = 134/475 (28.21%), Postives = 227/475 (47.79%), Query Frame = 0

Query:   78 GLKRGDKIHLFLGPYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICC---VQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWAITESESSMGPDLVDVN-NDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTD-------IPTTGMLVFNLVPFSLQSGFFFALDALKRSQMSVNNFGADTMSAIIEGCPKWK-PIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSA---------PT-CKHLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
            G+++GD I + L       F   MG    G   +  +P    Q +  QL+++  K I       D +      L L   + P PE       +  +  IT+ E++   + VD+  +D AA+ +SSGTTG PKG+  TH S         D       + +  +++  L  F + S     L++L RS  +V       + A+++   + +  IA ++P  ++       K  + N+  L  ++F++   A +   L+DS+R+ LP    +   +G  E G  +S          PT     G +    + KVVH++T   LG  + GE+C++  Q MKEYLN PE + A  D EG+ H GD+ Y D +  IF + RLKE++K++   V P ELES+LI H ++ D  V  + +DEV   +    V+ ++  +  E  V E   +V +QV  +KR+   + F++ + ++  GK+ R
Sbjct:   96 GIRKGDVIMILLQNSAEFVFSF-MGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGENLTLITTDEPTPENC-----LPFSTLITDDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL-RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIA----LAKNPTVNSYDLSSVRFVLSGAAPLGKELQDSLRRRLPQ-AILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQ-NDEVAGEVPVAFVVRSNGNDITEEDVKE---YVAKQVVFYKRLHK-VFFVASIPKSPSGKILR 553          

BLAST of amp dependent coa ligase vs. SwissProt
Match: gi|75296548|sp|Q7XXL2.2|4CLL9_ORYSJ (RecName: Full=4-coumarate--CoA ligase-like 9)

HSP 1 Score: 152.91 bits (385), Expect = 6.760e-39
Identity = 137/507 (27.02%), Postives = 236/507 (46.55%), Query Frame = 0

Query:   58 YSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPI-AMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQ---DTVPLAHSALHLFGPESPKPEVIIMDVDMVENWAITESESSMGPDLVDVN----NDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSS---TDIPTTGMLV-FNLVPFSLQSGFFFALDALKRSQMSV---NNFGADT-MSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDL--KFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAPTCKH--------------LGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQ 532
            Y+  +  +D+ +F    R  G+++G  + + L   N   +P+ ++GI   G   S  +P      I  Q+++++ K +   +   D V  A   +   G     P  I  D        +  +    G  +V V+    +DL A+ YSSGTTG  KG+  +H +  +   SS       T G +V   L+PF    G      A  R + +V   + F   T + A+++    + P  L+ P  L +     +K    +   L DL  K ++ A A +   L  + ++  P ++ +E  +G  E     S  T  H              +G+I P ++ K V  DTG+ L     GELCV++   M+ Y    E ++   D +G+ H GD+ Y D +G +F + R+KE++KY+   VAP ELE++L++HP+V+D  VFG P +E  ++  AC+V    + E  E    E+  +V E+V  +KR+R  +  +  + ++  GK+ R Q
Sbjct:   62 YTYGEVARDTARFARALRSVGVRKGHVVVVAL--PNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSEAKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWD-------GLLAAADRTGAGVVPVDAAQQSDLCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGICCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAP--LVPPVMLAM-----VKSPVADEFDLSDLALKSVMTAAAPLAPDLLAAFQRKFPGVQ-VEEAYGLTE----HSCITLTHAAGDGHGHVAKKSSVGFILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVGYIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVACVVRRHGAEEGEE----EIVAYVAERVASYKRVR-VLHIVDAIPKSVSGKILRRQ 542          

BLAST of amp dependent coa ligase vs. SwissProt
Match: gi|266484|sp|Q01158.1|LUCI_LUCLA (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 152.14 bits (383), Expect = 1.126e-38
Identity = 115/360 (31.94%), Postives = 175/360 (48.61%), Query Frame = 0

Query:  185 VDVNN--DLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTD-----IPTTGMLVFNLVPFSLQSGFFFALDALK---RSQMSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGET-GLFMSAPTCKHL----GWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVD-ELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKL 528
            V+VN    +A I  SSG+TG PKG+  TH +       + D       + G  +  +VPF    G F  L  L    R  M +  F  +T    ++    +K  ++I+   L   LN        +   LV+   I   GA +   + ++V +   NL G+   +G  ET    +  P         G + P  +AKV+ +DT K LGP   GE+CVK P  MK Y++ PE ++ + D EG+ H GD+ YYD E   F + RLK ++KY+   V P ELES+L+ HP + D  V G P     +L  A +VL     E  +S+ + E+  +V  QV + KR+RG + F+ E+ +   GK+
Sbjct:  186 VEVNRKEQVALIMNSSGSTGLPKGVQLTHENAVTRFSHARDPIYGNQVSPGTAILTVVPFHHGFGMFTTLGYLTCGFRIVM-LTKFDEETFLKTLQ---DYKCSSVILVPTLFAILNRSELLDKYDLSNLVE---IASGGAPLSKEIGEAVARRF-NLPGVRQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKTLGPNRRGEVCVKGPMLMKGYVDNPEATREIIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPIAGELPGAVVVL-----EKGKSMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKI 532          

BLAST of amp dependent coa ligase vs. SwissProt
Match: gi|158564046|sp|Q84P21.2|4CLL5_ARATH (RecName: Full=4-coumarate--CoA ligase-like 5; AltName: Full=4-coumarate--CoA ligase isoform 9; Short=At4CL9; AltName: Full=Peroxisomal OPC-8:0-CoA ligase 1)

HSP 1 Score: 151.754 bits (382), Expect = 1.375e-38
Identity = 132/520 (25.38%), Postives = 238/520 (45.77%), Query Frame = 0

Query:   49 LFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIA-MGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWAIT---------ESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTS-----------FWNWTGSSTDIPTTGML-VFNLVPFSLQSGFFFALDALKRSQMSVNNFGADTMSAIIEGCPKWKPIAL-IIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAPTCKH------LGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQTFGIIQS 539
              D ++  + + ++  +      D     G+++G  + L L P N I FP+  + +   G  I+  +P      IA Q+++++          +P   +A       + K  +++MD + V++             E   +   + VD  +D A + YSSGTTG  KG+  +H +           F +  G    I T  M  ++ L  F+        L A   + + ++ F    M  ++    K++  +L ++P  L+  +N   +  +K    L  +  ++  GA +   + +   +  P ++ ++    T  TG+  S  T +        G ++  ++ ++V   TG+ LGP + GEL +K P  MK Y +  E + +  DSEG+   GD+ Y D +G IF + RLKE++KY+   VAP ELE++L+THP + D  V   P  EV     A +V  T S  + +++++    FV +QV  +KRIR  + F+S + +N  GK+ R     I  S
Sbjct:   49 FIDASTGQNLTFTELWRAVESVADCLSEIGIRKGHVV-LLLSP-NSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAA-------AKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKERVD-QDDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAAFAT------GLLAYGSTIIVLSKF---EMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYD--LSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLSASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALLLTHPEITDAAVIPFPDKEVGQFPMAYVVRKTGSSLSEKTIME----FVAKQVAPYKRIR-KVAFVSSIPKNPSGKILRKDLIKIATS 542          

BLAST of amp dependent coa ligase vs. SwissProt
Match: gi|75290242|sp|Q6ETN3.1|4CL3_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; Short=Os4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 151.369 bits (381), Expect = 2.498e-38
Identity = 140/512 (27.34%), Postives = 225/512 (43.95%), Query Frame = 0

Query:   49 LFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMD------VDMVENWAITE--SESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGM------LVFNLVP----FSLQSGFFFALDALKRSQMSVNNFGADTMSAIIEGCPKWK-PIALIIPAFLMVPLNHKLKQSSKNT-ERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETG--LFMSAPTCKH--------LGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
            L D  +   Y+ ++    S +     R  G+ +GD +   L      AF   +G    G   +  +P      +  Q + A  + I     T   A   +  F  E   P V+ +D      V+  E  A  E  +++ + PD      D+ A+ YSSGTTG PKG+  TH S         D     +      ++  L+P    +SL S     L    R+  ++       + A+++   K    IA  +P     P+  ++ +S + T E L  ++ ++   A +   L+D+    +PN   +   +G  E G  L M     K          G +    + K+V  DTG  LG  ++GE+C++  Q MK YLN PE ++   D +G+ H GD+ + D +  IF + RLKEI+KY+   V P ELE++LITHP +KD  V     D   ++  A IV      E +E   DE+  FV ++V  +KRI   + F   + +N  GK+ R
Sbjct:   44 LIDGQTGESYTYAEVESASRRAAAGLRRMGVGKGDVVMSLLRNCPEFAFSF-LGAARLGAATTTANPFYTPHEVHRQAEAAGARVIV----TEACAVEKVREFAAERGVP-VVTVDGAFDGCVEFREVLAAEELDADADVHPD------DVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGENPNLYFSKDDVILCLLPLFHIYSLNSVLLAGL----RAGSTIVIMRKFDLGALVDLVRKHNITIAPFVP-----PIVVEIAKSPRVTAEDLASIRMVMSGAAPMGKDLQDAFMAKIPNAV-LGQGYGMTEAGPVLAMCLAFAKEPFKVKSGSCGTVVRNAELKIVDPDTGTSLGRNQSGEICIRGEQIMKGYLNDPEATKNTIDEDGWLHTGDIGFVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDDLAGEVPVAFIVRT----EGSEITEDEIKKFVAKEVVFYKRIN-KVFFTDSIPKNPSGKILR 528          

BLAST of amp dependent coa ligase vs. SwissProt
Match: gi|76789647|sp|Q42982.2|4CL2_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 2; Short=4CL 2; Short=Os4CL2; AltName: Full=4-coumaroyl-CoA synthase 2)

HSP 1 Score: 149.443 bits (376), Expect = 1.254e-37
Identity = 112/385 (29.09%), Postives = 183/385 (47.53%), Query Frame = 0

Query:  173 ITESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPT------TGMLVFNLVP----FSLQSGFFFALDALKRSQMSVNNFGADTMSAIIEGCPKWK-PIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTG----ETG-------LFMSAPT---CKHLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQ 532
            I +++    P++    +D  A+ +SSGTTG PKG+  TH S  +      D          G +   ++P    FSL S    A+    R+  +V       M A++    +W+  +A ++P  ++       K        L  ++ ++   A +   LED++R  LP     + IFG G    E G        F   PT       G +    + KVV  DTG  LG    GE+C++ PQ MK YLN PE + A  D EG+ H GD+ Y D +  +F + R+KE++K++   V P ELES+LI HP++ D  V  +  D   ++  A +V   DS    ES+ +    F+++QV  +KR+   + F+  + +++ GK+ R +
Sbjct:  195 IADADEGSVPEVAISPDDPVALPFSSGTTGLPKGVVLTHRSVVSGVAQQVDGENPNLHMGAGDVALCVLPLFHIFSLNSVLLCAV----RAGAAVALMPRFEMGAMLGAIERWRVTVAAVVPPLVLA----LAKNPFVERHDLSSIRIVLSGAAPLGKELEDALRARLP-----QAIFGQGYGMTEAGPVLSMCPAFAKEPTPAKSGSCGTVVRNAELKVVDPDTGFSLGRNLPGEICIRGPQIMKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELESLLIAHPSIADAAVVPQKDDVAGEVPVAFVVRAADSDITEESIKE----FISKQVVFYKRLHK-VHFIHAIPKSASGKILRRE 561          

BLAST of amp dependent coa ligase vs. SwissProt
Match: gi|158564048|sp|Q84P24.2|4CLL6_ARATH (RecName: Full=4-coumarate--CoA ligase-like 6; AltName: Full=4-coumarate--CoA ligase isoform 7; Short=At4CL7)

HSP 1 Score: 147.902 bits (372), Expect = 3.885e-37
Identity = 123/477 (25.79%), Postives = 223/477 (46.75%), Query Frame = 0

Query:   78 GLKRGDKIHLFLGPYNPIAFP-IAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTV-PLAHSALHLFGPESPKPEVIIMDVDMVEN---WAITESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTS-------FWNWTGSSTDIPTTGMLVFNLVPFSLQSG---FFFALDALKRSQMSVNNFGADTMSAIIEGCPKWKPIAL-IIPAFLMVPLNHKLKQSSKNT--ERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGL----FMSAPTCKH--LGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
            G+++GD + L L   N + FP I + +   G  ++  +PS  L  I  Q+    +       + V  L+   + +        E    D   +EN   ++I +      P  +   +D+AAI YSSGTTG  KG+  TH +       F  +  S  + P +  +    +P     G   F   L +L  + + +  F A  +  +IE   ++K     ++P  LM      L + +K    E    LK +    A +     +   Q LP+++ I+    T  T +    F S    ++  +G + P +QAKVV   +G  L PG  GEL ++ P  MK YLN P+ +Q     + +   GD+ Y+D +G +F + R+KEI+KY+   +AP +LE++L++HP + D  V   P++E  ++  A +V   ++  + E ++     +V  QV  ++++R  ++ ++ + ++  GK+ R
Sbjct:   94 GVRQGDVVSLVLP--NSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQVSECSVGLAFTSTENVEKLSSLGVSVISVS----ESYDFDSIRIENPKFYSIMKESFGFVPKPLIKQDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLCHIYGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIE---RFKITHFPVVPPMLMA-----LTKKAKGVCGEVFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYSSVGLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRTGDIAYFDEDGYLFIVDRIKEIIKYKGFQIAPADLEAVLVSHPLIIDAAVTAAPNEECGEIPVAFVVRRQETTLSEEDVIS----YVASQVAPYRKVRK-VVMVNSIPKSPTGKILR 551          

BLAST of amp dependent coa ligase vs. nr
Match: gi|1270057229|ref|XP_022791010.1| (uncharacterized protein LOC111330421 [Stylophora pistillata] >gi|1263129167|gb|PFX25441.1| 4-coumarate--CoA ligase [Stylophora pistillata])

HSP 1 Score: 206.453 bits (524), Expect = 1.453e-53
Identity = 150/516 (29.07%), Postives = 250/516 (48.45%), Query Frame = 0

Query:   45 NNKWLFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLG--PYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENW-AITESESSMGPDL-VDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGMLVFNLVPFSLQSGFFFALDALKRSQMSVNNFGADTMSAIIEGCPKWKP--------------IALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAPTC--------KH--LGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQ 532
            + + + D  SD  YS  Q    S + +     +G K+G+   +FL   P  PI F    G+   GG ++  +P    + +A QL+ AD K+I  V   V  A  A+   G E+     +I D+D  E+  A+ + + S  PD+ +    D A + +SSGTTG PKG+  TH   +N     + + T G L F+     L    FF +  +    +++ + G    SAII   P+++               +  + P  L++      K  S +   L  L  +    A +   +  ++   LP L  +   +G  E      +P C        KH  +GW+ P ++ KVV ++TG+ LGPG  GE+CV+ P  MK YLN PE +    D++G+ H GD+ +YD +   F + R+KE++KY+   V P ELE++LI+HPA++D  V G P  E  +L  A +V   D     +    ++  FV+E +  +K++RG + F+ ++ ++  GK+ R +
Sbjct:  572 DREAIRDSISDRSYSYRQIKDLSKRISSALNKRGFKKGEVFAMFLPNLPEFPIVF---FGVLQAGGVVTAANPLFTTEELAKQLKLADAKWILTVPQLVHRAKEAMQDLGREN---MFVIGDIDGCESLSALLQDDGSSFPDVTIKPREDTAVLPFSSGTTGHPKGVMLTH---YNLVSCGSMMRTEGFLSFSDTTVLLAFLPFFHVYGM----VAIMSMGLRCGSAIIT-MPRFERGKFLQTLQNYEVTHLPAVPPVVLLLA-----KHPSVDNFDLSYLTEVTNGAAPLGKQVSKALMDRLPLLSSVRQGYGMTEL-----SPVCHMTPAENNKHGSVGWVLPNLRCKVVDVETGEALGPGHDGEICVRGPTVMKGYLNNPEATAQTIDADGWLHTGDIGHYDEDEHFFIVDRVKELIKYKGYQVPPAELEALLISHPAIEDAAVIGVPDAEAGELPMAFVVRKADVTLTEK----DVHVFVDENLAAYKKLRGGVEFVDQIPKSLSGKILRRE 1059          

HSP 2 Score: 159.844 bits (403), Expect = 8.746e-38
Identity = 141/511 (27.59%), Postives = 237/511 (46.38%), Query Frame = 0

Query:   49 LFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWA-ITESESSMGPDLVDVN--NDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGMLVFNLVPFSLQSGFFFALDALKRSQMSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQS------------SKNTER--LVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAPTCK----------HLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQ 532
            + D  S   Y+  Q ++ + K       +G K+GD   + L P  P    I       GG ++  +P   +  I++QL++A    I  +    P A  A       +     ++ + +  E+ + + E +    P+ V++N   D+A + YSSGTTG PKG+  TH   +N    ++ +     +  N  P  L    FF +     S MS   F   T+  +    PK++P      +FL V  ++K+  +            S   E+  L  LK +    A +   L +S+++ +P++E     FG  E      +PT             +G + P ++ KVV I+ G  LGP + GE+CVK P  MK YLN PE ++   D++G+ H GD+  YD E   F + RLKE++K++   V P ELES+LI+H A++D  V G P     +L  A +V  T+     E ++  +     E+V   K ++G + F+ ++ R+  GK+ R +
Sbjct:   35 IVDGPSGRSYTFQQLIQLTRKCGSALTKRGFKKGDVFAILL-PNMPEFTIIYYSTLLIGGIVTTLNPLYTVDEISHQLRDAGATSIITIP---PFAEKAKQSATNVAINTVYVVGEAENFESLSSLLEDKGEDFPENVEINPKEDVACLPYSSGTTGAPKGVMLTH---YNLIAEASIVTHDSFMFNNDEPVVLGLLPFFHIFGQIIS-MSCALFKGGTVVCV----PKFEP-----DSFLKVIQDYKVSHAAVVPSVVLFLAKSPLVEQYDLSSLKDLTSGAAPLGGELMNSLKERIPSIEWCRQGFGMTEL-----SPTSHVTPLDNVENGSVGVLLPNLECKVVDIENGNELGPNKEGEICVKGPTVMKGYLNNPEATKQTIDTDGWLHTGDIGRYDDEENFFIVDRLKELIKFKGFQVPPAELESLLISHSAIEDAAVIGVPDVRAGELPKAFVVKKTNEVITEEEIIKFIE----ERVAPHKTLKGGLEFIDKIPRSLSGKILRRE 519          

BLAST of amp dependent coa ligase vs. nr
Match: gi|747089862|ref|XP_011092586.1| (4-coumarate--CoA ligase-like 6 [Sesamum indicum] >gi|922074205|gb|ALA55541.1| 4-coumarate CoA ligase [Sesamum indicum])

HSP 1 Score: 199.134 bits (505), Expect = 5.409e-53
Identity = 153/492 (31.10%), Postives = 250/492 (50.81%), Query Frame = 0

Query:   74 FRHKGLKRGDKIHLFLGPYNPIAFPIA-MGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALH---LFGPESPKPEVIIMDVDMVENWAI----TESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFW-------NWTGSSTDIPTTGMLVFNLVPFSLQSG---FFFALDALKRSQMSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGL----FMSAPTCKH--LGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQTFGIIQSLL 541
              H G+K+GD I LFL P N + FPI  +G    G  ++  +P   L  I  Q+ +++   +  V D V     AL    + G     PE++  D  + +++++      S+  M P    +  D AAI YSSGTTG+ KG+  TH +F         +  S  D P+TG +   +VP     G   F   L +L  + +++  F AD M   I+   ++    L     L+  L  K K++  N+ R   LK +    A + +   +   + LP+++ I+    T  T +    + +A   K+  +G ++P VQAKVV   TG  L PG  GEL ++TP  MK YLN  E + A  D +G+ H GD+VY+D EG ++ + RLKE++KY+   +AP +LE++LI+HP V D  V G   +E  ++  A +VL   S  +  +L+D    +V +QV  +K++R  + F + + R++ GK+ R +   ++ S L
Sbjct:  104 LHHMGVKQGDVI-LFLLP-NSVCFPIVFLGALSVGAIVTPMNPLSSLSEIKRQVLDSNASLVFSVLDRVHELFGALRGCSVIG----VPEILSFD-SLPDDYSVFHKLVSSDPRMAPTPEIMQQDTAAILYSSGTTGRAKGVMLTHGNFIAMMELFVRFEASLYDYPSTGHVYLAVVPMFHVYGLSLFVMGLLSLGITVVAMKKFDADEMVRAID---RYGVTHLHAVPPLLTALTRKAKRADGNSFR--SLKQVSCGAAPLSEKSINEFIEALPHVDFIQGYGMTESTAVGTRGYNTAKVQKYTSVGLLSPNVQAKVVDWVTGSHLPPGSVGELWLRTPGTMKGYLNNIEATTAAIDKDGWLHTGDIVYFDQEGYLYIIDRLKEMIKYKGFQIAPADLEAVLISHPEVLDAAVTGAKDEEAGEIPVAFVVLKDGSSVSGAALLD----YVAKQVAPYKKVR-KVYFRTSIPRSAAGKILRRELRSLLVSRL 578          

BLAST of amp dependent coa ligase vs. nr
Match: gi|1176095348|ref|XP_020618234.1| (4-coumarate--CoA ligase 4-like [Orbicella faveolata])

HSP 1 Score: 201.06 bits (510), Expect = 9.432e-52
Identity = 156/516 (30.23%), Postives = 251/516 (48.64%), Query Frame = 0

Query:   45 NNKWLFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIA-MGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWA-ITESESSMGPDL-VDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGMLVFNLVPFSLQSGFFFALDALKRSQMSVNNFGADTMSAIIEGCPKWKP--------------IALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAPTC--------KH--LGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVD-ELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQ 532
            + + + D  +D  YS  Q    S +       +GLK+GD   + L P  P  FPI   G+   GG I+  +P    + +A QL+ AD K+I  V   V  A  A+   G  +     +I DVD  E+ + +   + S  PD+ ++   D+A + +SSGTTG PKG+  TH    N     + + + G L F+     L    FF         +++ + G    S II   P+++               + ++ P  LM+      K  S +   L  L  +    A +   L  +++  LP L  I   +G  E      +P C        KH  LG + P ++ KVV ++TG+ LG G+ GE+CVK P  MK YLN PE +    DS+G+ H GD+ +YD +   + + R+KE++KY    V P ELE++LI+HP ++D  V G P+ EV +L  A +V      +AN +L + ++  FV+E V  +K++RG + F+ E+ ++  GK+ R +
Sbjct:  573 DREAMRDSITDRSYSYRQVKDLSKRIASALNKRGLKKGDVFAMLL-PNLP-EFPIVYFGVLQAGGVITAANPLFTTEELAKQLKQADTKWILTVPQLVHRAKEAMQELGRSNV---FVIGDVDGCESLSGLLADDGSSFPDVAINPKEDIAVLPFSSGTTGHPKGVILTH---HNLISCGSIMRSKGFLSFDETTVLLAFLPFFH----SYGMVAIMSMGLHCGSTIIT-MPRFEREKFLHALQNYEVTHLPVVPPVVLMLA-----KHPSVDNFDLSYLTEVTSGAAPLGKELSQALKDRLPLLSSIRQGYGMTEL-----SPVCHMTPADNNKHGSLGVLLPNLRCKVVDVETGENLGHGQDGEICVKGPTVMKGYLNNPEATAQTIDSDGWLHTGDIGHYDEDEHFYIVDRVKELIKYNGYQVPPAELEALLISHPDIEDAAVIGIPNLEVGELPMAFVVR-----KANATLTEKDVEIFVDENVAPYKKLRGGVEFIDEIPKSLSGKILRRE 1060          

HSP 2 Score: 181.03 bits (458), Expect = 7.650e-45
Identity = 152/516 (29.46%), Postives = 245/516 (47.48%), Query Frame = 0

Query:   49 LFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIAM-GIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWAITESESSM----GPDL---VDVN--NDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGML--------VFNLVPFSLQSGFFFALD-ALKRSQ--MSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAPTCK----------HLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLV-DELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQ 532
            + D  S   Y+  Q    + KF      KG K+GD   + L P  P  FPI   G+ + GG ++  +P   ++ I +QLQ+A +  I     T+PL    +     +     V I  V +V      ES S++    G D    V++N   D+A + YSSGTTG PKG+  TH   +N    +  +     +        V  L+PF    G   ++  AL +    + V  F  ++   II+   K    AL+    L +  +  ++Q       L  LK +    A + + L  S+++ LP++E     +G  E      +P C            +G + P ++ KVV I+ G  L P + GE+CVK P  MK YLN PE +    +S+G+ H GD+ YYD +   F + RLKE++KY+   VAP ELE++L++H A++D  V G P     +L  A +V      +ANE +  +E+  ++ E+V   K +RG + F+ ++ R+  GK+ R +
Sbjct:   35 IVDGPSGRSYTFEQLAVLTKKFASALTKKGFKKGDVFAILL-PNIP-EFPIVYYGVIFIGGVVTTMNPLYTVEEITHQLQDAGVTSII----TIPLFADKV-----KESTSNVGINTVYVVGEAEGCESLSALLQDDGEDFPKNVEINPKEDVACLPYSSGTTGVPKGVMLTH---YNLIACALIVTHESFMWNKEEDAAVLGLLPFFHSFGQVISMSCALYKGAKVVCVPKFEPESFLKIIQEH-KVSHAALVPSTVLFLSKSPLVEQYD-----LSSLKDVTSGAAPLGEELSKSLKERLPSIEWFRQGYGMTEL-----SPVCHVTPFDNIKYGSVGVLLPNLECKVVDIENGDELAPNKEGEICVKGPTVMKGYLNNPEATAKTIESDGWLHTGDIGYYDEDEHFFIVDRLKELIKYKGFQVAPAELEALLVSHTAIEDAAVIGVPDARAGELPKAFVVK-----KANEEITEEEIAKYIEERVAPHKNLRGGVEFIDKIPRSLSGKILRRE 520          

BLAST of amp dependent coa ligase vs. nr
Match: gi|1270071368|ref|XP_022798582.1| (4-coumarate--CoA ligase 1-like [Stylophora pistillata])

HSP 1 Score: 194.126 bits (492), Expect = 1.571e-49
Identity = 156/528 (29.55%), Postives = 247/528 (46.78%), Query Frame = 0

Query:   24 IPNGSIYVELDRIVRQRNLKTNNKWLFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLG--PYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWAITESESSMG-PDLVDVN--NDLAAIFYSSGTTGQPKGICHTHTSFW---------NWTGSSTDIPTTGMLVFNLVPFSLQSGFFFALDALKRSQ--MSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVP--LNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETG-LFMSAP----TCKHLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKL 528
            +PN S+    D  ++ +NL        D  S+  ++ ++      KF      +G ++GD   L+L   P  P++F    G+   GG ++  +P+   + IA QLQN+  K+I  V +    A  A    G    K   +I + +  E  A   S+  M  PD V +N   D+ A+ YSSGTTG PKG+  TH +           N+     +  T G+L F    F +       L  L R    + +  F  + M  +I+ C         I    +VP  +    K  S +   L  +  I+   A +   L ++V + LP +  +   +G  ET    M  P    T   +G + P ++ KVV + TG+ LGP + GE+CV+ P  MK YLN P+ ++     +G+ H GD+ YYD EG  F + RLKE++KY+   V P ELE++L THP V D  V G P +E  +L  A IV   D  E       E+  +V ++V   K++RG + F+ ++ + + GK+
Sbjct:  431 LPNRSV----DPAIQAKNLS------IDGVSEKSFTYNELSTSVKKFASALTRRGFQKGDVFALYLPNVPEYPVSF---FGVIAIGGTVTTLNPTFTEKEIAYQLQNSGAKFILTVPEIADKARQAATEAGI---KEVFVIGEAEGCEPLASLLSDDGMAFPDNVAINPKEDICAMPYSSGTTGFPKGVMLTHHNIVSQLCILMHENFMSQPQEATTLGLLPF----FHIFGMVVLMLQHLYRGGKIVCLQKFEGEHMLKLIQDCQ--------IHVLYLVPPIVTFLAKHPSVDDYDLSSVTTIISGAAPLGLELTNAVWKRLPKVAVVGQGYGLTETSPAVMLCPREDCTPGAVGVMLPNIEGKVVDVQTGQPLGPNQNGEVCVRGPVVMKGYLNNPKATEECITPDGWFHTGDIGYYDQEGHFFIVDRLKELIKYKGFQVPPAELEALLHTHPNVLDVAVVGIPDEEAGELPKAFIVPKGDITER------EIIEYVADRVSPHKKLRGGVEFIEQIPKTASGKI 924          

BLAST of amp dependent coa ligase vs. nr
Match: gi|1263124134|gb|PFX20569.1| (4-coumarate--CoA ligase 1 [Stylophora pistillata])

HSP 1 Score: 191.815 bits (486), Expect = 7.738e-49
Identity = 150/502 (29.88%), Postives = 236/502 (47.01%), Query Frame = 0

Query:   50 FDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLG--PYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWAITESESSMG-PDLVDVN--NDLAAIFYSSGTTGQPKGICHTHTSFW---------NWTGSSTDIPTTGMLVFNLVPFSLQSGFFFALDALKRSQ--MSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVP--LNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETG-LFMSAP----TCKHLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKL 528
             D  S+  ++ ++      KF      +G ++GD   L+L   P  P++F    G+   GG ++  +P+   + IA QLQN+  K+I  V +    A  A    G    K   +I + +  E  A   S+  M  PD V +N   D+ A+ YSSGTTG PKG+  TH +           N+     +  T G+L F    F +       L  L R    + +  F  + M  +I+ C         I    +VP  +    K  S +   L  +  I+   A +   L ++V + LP +  +   +G  ET    M  P    T   +G + P ++ KVV + TG+ LGP + GE+CV+ P  MK YLN P+ ++     +G+ H GD+ YYD EG  F + RLKE++KY+   V P ELE++L THP V D  V G P +E  +L  A IV   D  E       E+  +V ++V   K++RG + F+ ++ + + GK+
Sbjct:  397 IDGVSEKSFTYNELSTSVKKFASALTRRGFQKGDVFALYLPNVPEYPVSF---FGVIAIGGTVTTLNPTFTEKEIAYQLQNSGAKFILTVPEIADKARQAATEAGI---KEVFVIGEAEGCEPLASLLSDDGMAFPDNVAINPKEDICAMPYSSGTTGFPKGVMLTHHNIVSQLCILMHENFMSQPQEATTLGLLPF----FHIFGMVVLMLQHLYRGGKIVCLQKFEGEHMLKLIQDCQ--------IHVLYLVPPIVTFLAKHPSVDDYDLSSVTTIISGAAPLGLELTNAVWKRLPKVAVVGQGYGLTETSPAVMLCPREDCTPGAVGVMLPNIEGKVVDVQTGQPLGPNQNGEVCVRGPVVMKGYLNNPKATEECITPDGWFHTGDIGYYDQEGHFFIVDRLKELIKYKGFQVPPAELEALLHTHPNVLDVAVVGIPDEEAGELPKAFIVPKGDITER------EIIEYVADRVSPHKKLRGGVEFIEQIPKTASGKI 874          

BLAST of amp dependent coa ligase vs. nr
Match: gi|1005487375|ref|XP_015780279.1| (PREDICTED: 4-coumarate--CoA ligase-like 7 [Acropora digitifera])

HSP 1 Score: 191.045 bits (484), Expect = 2.139e-48
Identity = 145/509 (28.49%), Postives = 243/509 (47.74%), Query Frame = 0

Query:   51 DYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLG--PYNPIAFPIAMGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWA--ITESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGMLVFNLVPFSLQ-SGFFFALDALKRSQM---------SVNNFGADTMSAIIEGCPKWKPI--ALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAPTCK----------HLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVD-ELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQ 532
            D  +   YS  +      K       KG ++GD   LFL   P  PIAF    G+   GG ++  +P    + +A QL+ AD K+I  V   V  A  A+   G ++    +++ +VD  E+ +  + +  SS+    ++   D A I +SSGTTG PKG+  TH   +N     + +   G L F+     L    FF +   +    M         S+  F  +    ++E   K++    +++ P  L++      K  + +   L +L  I    A + + +   V + LP L  +   +G  E      +P C            +G + P ++ KV+ ++TG+ LGPG  GE+CVK P  MK YLN PE +    DS+G+   GD+ +YD +   F + R+KE++KY+   V P ELE++L++HP ++D  V G P  E  +L  A +V      + N +L + ++  FV+E V  +K++RG + F+ E+ R+  GK+ R +
Sbjct:  456 DCVTKRSYSFREIKVLCRKCASGLSKKGFQKGDVFALFLPNLPEFPIAF---FGVLQAGGVVTSANPLFTTEELAKQLKQADAKWILTVPQLVHRAKEAMQELGRDN---VLVVGEVDGYESMSELLKDDGSSVPEPSINPKEDTAVIPFSSGTTGHPKGVMLTH---YNLIACGSMMRAQGFLSFDETTVLLAFLPFFHSFGMVANMSMGLHTGSFLVSMPRFDKEKFLQVLE---KYEVTHSSVVPPVVLLLA-----KHPAVDNYDLSNLTEITNGAAPLGEEISKEVMKRLPLLSRVRQGYGMTEL-----SPVCHMTPFENNKLGSVGMLLPNLRCKVIDLETGEALGPGHDGEICVKGPTVMKGYLNNPEATAQTIDSDGWLCTGDVGHYDEDEHFFIVDRVKELIKYKGYQVPPAELEALLVSHPDIQDAAVVGVPDVEAGELPMAFVVR-----KPNAALTERDVKTFVDENVAPYKKLRGGVEFIDEIPRSLSGKILRRE 937          

HSP 2 Score: 153.68 bits (387), Expect = 7.801e-36
Identity = 109/367 (29.70%), Postives = 182/367 (49.59%), Query Frame = 0

Query:  182 PDLVDVN--NDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGML-------VFNLVPFSLQSGFFFALDA--LKRSQM-SVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAPTCKHL-----GWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDE-LTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
            P  V++N  +D+A + YSSGT G PKG+  TH   +N    +  +     L       V  L+PF    G    L    LK   +  V  F  ++   I++   K    AL+ P  L +  +  + Q       L  L+ +    A + + L  ++ + +P+++     +G  E      A  C  +     G + P ++ KV++++ G  LGP + GE+CV+ P  MK YLN  E +  + DS+G+ H GD+ +YD +   +   RLKE++K +   V P ELE++L++HPA+ D  V G P  E  +L  A +V      +ANE++ D+ L  F+ E+V  +K + G + F+ E+ R+  GK+ R
Sbjct:   25 PKDVEINPKDDIACLAYSSGTMGSPKGVMLTH---YNLIADALLVTHESFLWMNEHPVVLGLLPFFHAFGQINVLSCPLLKGGTLVCVPKFEPESFLKIMQDF-KVTHAALVPPTVLFLAKSPLIDQFD-----LSSLEDVSSGAAPLGEDLAKTLTERIPSIKWFRQGYGMTELSPVSHATPCDQVKYGSVGVLLPNLECKVINVEDGTELGPNKDGEICVRGPTVMKGYLNNQEATAQMIDSDGWLHTGDIGHYDQDEHFYITDRLKELIKCKGYQVPPAELENLLVSHPAITDAGVIGVPDSEAGELPKAYVVR-----KANENITDQDLVKFIEERVAPYKALGGGVQFIDEMPRSLSGKILR 377          

BLAST of amp dependent coa ligase vs. nr
Match: gi|568252170|gb|ETN61534.1| (AMP dependent coa ligase [Anopheles darlingi])

HSP 1 Score: 185.267 bits (469), Expect = 4.384e-48
Identity = 143/512 (27.93%), Postives = 235/512 (45.90%), Query Frame = 0

Query:   49 LFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIA-MGIWYNGGCISLGDPSLKLQTIANQLQ----------NADIKYICCVQDTVPLAHSALHLFGPESPKP-EVIIMDVDMVENWAITESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDIPTTGML-----VFNLVPFSLQSGFFFALDA-LKRSQMSV-NNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGLFMSAPT---CKHLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQTFGIIQ 538
            L D  +    +  + L+ S K  + F   GLKRG  + +     N + FPI        GG I   +PS     +A+ LQ          +  +  I  ++D +P     + L  P +  P +  +  +D   + +   S +S  P  V +   +A +  SSGTTG PK +  TH +         + P    L     V  L+PF    GF  +L++ L R  M V +++        I+       +  ++P  ++    H L +       L  L  ++   A +   +ED VR  LPN   +   +G  ET L + +        +G ++     KV+ +DTG  LGPG+ GE+CVK P  MK YLN  + ++ + D++G+   GD+ YYD E   F + RLK+++KY+   V P ELE +L++HP V+D  V G P +   +L +A +VL  D+    E+   +L  +V  ++   K +RG + F+ E+ +   GKL R Q    +Q
Sbjct:   45 LVDGVTGQQCTYHEILERSLKLANRFHRLGLKRGSVVAI--ASENSLQFPIVTFATIMLGGTILPLNPSYTAGEMAHVLQLTKPWSIFASSKPLATIRAMRDRLPFVKLLVALIPPRTECPADAKLPTLDEFFDRSPLHSLTSFTPQPVRLKEHVAVMVMSSGTTGLPKAVQLTHHNVMTVMAYQAEDPRYTELPVPIRVLGLLPFYHVFGFMLSLNSCLNRVPMVVLSHYEPRLFLRTIQ--EHRITMVSLVPPLMVFLAKHPLVEEYD----LSSLHAVLSGAAPLSKEIEDLVRARLPNARTVRTGYGMSETSLGVISRVNDKVGSVGKVHKTTYVKVIDLDTGVPLGPGQTGEICVKGPLVMKGYLNNEQATREMLDADGWLRTGDIGYYDEEQDFFIVDRLKDLIKYKAFQVPPAELEDVLLSHPKVRDAAVVGLPDEAAGELPAAFVVLQPDTEPITEA---QLGQYVASKLSPHKWLRGGVYFVQEIPKTGSGKLLRRQLRQTLQ 545          

BLAST of amp dependent coa ligase vs. nr
Match: gi|642915003|ref|XP_008190478.1| (PREDICTED: 4-coumarate--CoA ligase 1 [Tribolium castaneum] >gi|642915005|ref|XP_008190479.1| PREDICTED: 4-coumarate--CoA ligase 1 [Tribolium castaneum] >gi|642915007|ref|XP_008190480.1| PREDICTED: 4-coumarate--CoA ligase 1 [Tribolium castaneum] >gi|270002347|gb|EEZ98794.1| Luciferin 4-monooxygenase-like Protein [Tribolium castaneum])

HSP 1 Score: 183.726 bits (465), Expect = 1.422e-47
Identity = 153/515 (29.71%), Postives = 235/515 (45.63%), Query Frame = 0

Query:   45 NNKWLFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIA-MGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICCVQDTVP-----------LAHSALHLFGPESPKPEVIIMDVDMVENWAITESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGS---------STDIPTTGMLVFNLVPFSLQSGFFFA-LDALKRSQMSV-NNFGADT-MSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGL-FMSAPTCK----HLGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
            +N    D  +D     +  L  S +   + R  G+K GD I +     N + F +  +  ++ G   +  +P      + + L+ +  K I C   T+            L H  L LFG +      +IM  D++        + S     VD  + +A I  SSGTTG PKG+  TH S   +              D P+  M+   L PF    GF    L+ L+  +M V + F     + AII    K+K   L++P  +M+ L   LK        L  +K I    A +   +E  ++     +  +   +G  ET L  + +P  K     +G I P + AKV+  DTGK LGP + GE+C K P  MK Y+  P  +    D +G+ H GD+ YYD +G  F + R+KE++KY+   VAP ELE++LITHPAV D  V G P +   +L  A +V   +    +E+   EL  FV + V   K++RG ++F+  + RN  GK+ R
Sbjct:   35 DNVLQVDAATDEELPANLLLSRSIQLAKWLRSIGVKEGDSISV--NSENRLEFAVVTVATFFVGAVFAPLNPEYTPGELNHVLKLSKPKVIFCSPQTIQTMTKVFADHPNLTH--LVLFGAQKRNESYVIMHEDIIRGATGDNIDESFEATPVDPKDAIATILMSSGTTGLPKGVMCTHESMTTYVDIMRVTMAQIIENDDPSDAMM--GLAPFFHSMGFMLMFLNLLRGKKMVVLSRFKTKIFLDAII----KYKISRLVVPPPVMLVL---LKHPLTKQYDLSGIKEIRTGAAPMGKDMERELKNRF-KVGHVSQGYGMTETTLGILVSPLGKTKVGSVGKIVPGMMAKVID-DTGKALGPYKEGEVCFKGPLIMKGYVGDPVATANTIDQDGWIHTGDVAYYDEDGYFFIVDRIKELIKYKGYQVAPAELEALLITHPAVADAAVIGLPDERAGELPLAFVVKKPN----HETTDKELEKFVADNVSSQKQLRGGVVFIDAIPRNPSGKILR 530          

BLAST of amp dependent coa ligase vs. nr
Match: gi|1276245422|gb|PIN05522.1| (Acyl-CoA synthetase [Handroanthus impetiginosus])

HSP 1 Score: 184.111 bits (466), Expect = 1.523e-47
Identity = 145/490 (29.59%), Postives = 242/490 (49.39%), Query Frame = 0

Query:   78 GLKRGDKIHLFLGPYNPIAFPIAMGIWYNGGCISLG-DPSLKLQTIANQLQ--NADIKY--ICCVQDTVPLAHSALHLFGPESPKPEVIIMDVDMVEN-----WAITESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWT-------GSSTDIPTTGMLVFNLVPFSLQSG---FFFALDALKRSQMSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGETGL----FMSAPTCKH--LGWINPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQRLQTFGIIQSLL 541
            G+K+GD I + L   N + FP+      + G I+   +P   L  I  Q    NA + +  +  V + V +    L +  PE       + D++ V N       +  S+ +M P  + +  D AAI YSSGTTG+PKG+  TH +F            S  D P+   +   +VP     G   F   L +L  + +++  F  D M   IE   ++    L     L+V L  + K+ ++++ R   LK +    A + +   +   ++ P+++ I+    T  T L    + S    K+   G ++P VQAKVV   TG  L PG  GEL ++TP  MK YLN P+ + A  D +G+ H GD+VY+D EG ++ + RLKE++KY+   VAP +LE++L+ HP V D  V G+  +E  ++  A +V    S  + ++L+D    +V +QV  +K++R  + + + + R++ GK+ R +   +  S L
Sbjct:  104 GVKQGDVILILLP--NSVCFPVIFLAALSVGAIATTMNPLSSLSEIKKQFSDSNASLAFSVLGKVDELVSILEGCLVVGVPE-------VFDLNYVPNDGSVFHKLISSDPNMAPRPMIMQRDTAAILYSSGTTGRPKGVMITHGNFIAMMELFVRSEASLYDFPSNASVYLAVVPMFHVYGLSLFAMGLLSLGSTVVTMKKFDIDEMVRAIE---RYGVTHLHAVPPLLVALTKRAKKDNRSSFR--SLKQVSCGAAPLSEKSINEFIEMFPDVDFIQGYGMTETTALGTRGYNSNKVHKYSSAGLLSPNVQAKVVDWATGSSLPPGSIGELWLRTPGNMKGYLNNPDATMAALDKDGWLHTGDIVYFDKEGYLYVIDRLKEVIKYKGFQVAPADLEAVLMAHPDVLDAAVIGEMEEEAGEIPVAFVVPKDGSSVSQQALMD----YVAKQVAPYKKVRR-VYYCASIPRSAAGKILRRELKSLFASRL 574          

BLAST of amp dependent coa ligase vs. nr
Match: gi|1133427647|ref|XP_019869694.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Aethina tumida])

HSP 1 Score: 182.956 bits (463), Expect = 2.662e-47
Identity = 157/542 (28.97%), Postives = 250/542 (46.13%), Query Frame = 0

Query:   25 PNGSIYVELDRIVRQRNL--------KTN--NKWLFDYTSDTHYSPSQFLKDSHKFNDFFRHKGLKRGDKIHLFLGPYNPIAFPIA-MGIWYNGGCISLGDPSLKLQTIANQLQNADIKYICC----------VQDTVPLAHSALHLFGPESPKPEVIIMDVDMVENWAITESESSMGPDLVDVNNDLAAIFYSSGTTGQPKGICHTHTSFWNWTGSSTDI---------PTTGMLVFNLVPFSLQSGFFFA-LDALKRSQMSVNNFGADTMSAIIEGCPKWKPIALIIPAFLMVPLNHKLKQSSKNTERLVDLKFIVPAGAAIPDSLEDSVRQLLPNLEGIENIFGTGET--GLFMSAPTCKHLGW---INPKVQAKVVHIDTGKRLGPGEAGELCVKTPQFMKEYLNLPELSQALFDSEGFAHVGDMVYYDHEGRIFYLQRLKEILKYRLKHVAPTELESILITHPAVKDCMVFGKPSDEVNDLISACIVLNTDSFEANESLVDELTGFVNEQVEDFKRIRGPIIFMSELERNSFGKLQR 530
            PN  +  EL+R+V + NL        KT+  N    D T+      ++ L  + K  ++F+  G+  GD + +     N + F I  +  ++ G   +  +P    + + + L  +  K + C          VQ+  P     L LFG E+     + +  D++ +      + +      D N  +A I  SSGTTG PKG+  TH S   +   +  +         P+  M+   LVPF    GF    L+ L+   M V           +E   K+K   LI+P  +++ L   LK        L  +K I    A +   +E  ++     ++ +   +G  ET  G+ M+ P    LG    I P   AK++  + G  LG  + GELC + P  MK Y+  PE +    D++G+ H GD+ YYD EG  F + RLKE++KY+   VAP ELE++LITHP+V+D  V G P+DE  +L  A +V  +      +    E+  FV + V   KR+RG +IF+ E+ RN+ GK+ R
Sbjct:    3 PNIIVGPELERVV-EGNLGELLLLLLKTSEENVIQIDATTGEKLLANELLVRAIKLAEWFKMNGIGVGDSVTV--SSENRLEFCIVPVAAFFVGATFAPINPDYTPRELLHVLSLSKPKVMFCSARTISKMLQVQNDHPYLQK-LILFGNETKPNGRVELFNDIIRDADPENIDEAFEAADYDPNEVVATILCSSGTTGMPKGVMCTHASMTAYVDIARAVMKDMVESEDPSDAMM--GLVPFFHSFGFMLMFLNVLRGKTMIV--LSKFHTKVFLEAIVKYKITRLIVPPPVLLIL---LKNPLTKNYDLSHIKEIRSGAAPLGKDMEQELKDRF-KVKNVSQSYGMTETTLGILMTPPGAGKLGSSGKIVPGCAAKIID-EKGNTLGANKEGELCFRGPLIMKGYVGDPEATANTIDADGWLHTGDVAYYDDEGYFFIVDRLKELIKYKGFQVAPAELEALLITHPSVQDAAVIGLPNDEAGELPLAYVVKKSGKTVTQQ----EIEKFVEDAVSPQKRLRGGVIFVEEIPRNATGKILR 527