BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000006113 (supercontig:LSalAtl2s:LSalAtl2s332:452120:457604:1 gene:EMLSAG00000006113 transcript:EMLSAT00000006113 description:"augustus_masked-LSalAtl2s332-processed-gene-4.0")

HSP 1 Score: 561.607 bits (1446), Expect = 0.000e+0
Identity = 286/609 (46.96%), Postives = 396/609 (65.02%), Query Frame = 0

Query:   41 NYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE-------------STLKHAHRLGVDP--EVSSDSALRKESPLSA----RMQSMHQHYPNLVDCYFETR-RSVASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRLGNKP 629
            ++ CP+CF++ KE  +T+CGHSFC  CL  +L+    +CPKC   ++   ++ PNF LN +  ++   QK                     + TP                 +  +NLS  D+  L G L + K+ + +    ++  LL EFL+ LK EK   L + +++ +++ +DL+             S         V P  EVS+ +   KE P  +    R + MH+H  +L   Y+ +R + ++  P   S   +  GL  FS  L+KFTKY+ ++ +A L+Y +DM++N SIVSSI+FDKD EFFAI GVTKRIK+YDY+ V+ D+VDIHYP  EM+S SKISC+SW+SFHK  L+SSDY+G+V +WDA +  K +++ EHEKRCWSVDFN +DTKL AS SDDARVKLWST+++HS+ASLEAKANVC VKFNP SC+H+AFGSADHCVHY+DLRN K+ L +F GHKKAVSYV+FLN+  ++SASTDSQLKLW+V +  C++S+ GH NEKNFVGL +D +Y   GSENN   ++YKGL K LFSFKFD+ +T   RN++  D +NEFVSA+CWR+GT+ ++ ANSQG +K+  L + P
Sbjct:    9 DFSCPVCFDILKEPHMTRCGHSFCYSCLNKSLK-IKPQCPKCSFDLHPDKDIYPNFTLNQIISKL---QKD--------------------NDTPQ----------------IPTSNLSMSDINRLLGTLHRRKKELEKESFLTQNELLAEFLSHLKVEKDAELLRIRRESEVIQADLDHVNQVLQDYRTNSSNFASGDENNVVPSLEVSNPTINIKEPPTESSFPMRRRRMHKHVLDLSSAYYSSRAKEMSFTPEECSSSLDYSGLKDFSLCLNKFTKYNTVKPLATLSYMTDMFNNTSIVSSIDFDKDNEFFAIGGVTKRIKIYDYSVVLNDLVDIHYPSMEMVSSSKISCISWSSFHKGTLVSSDYEGSVVVWDANSNSKIRTFQEHEKRCWSVDFNRIDTKLVASGSDDARVKLWSTNIHHSVASLEAKANVCCVKFNPESCYHLAFGSADHCVHYYDLRNTKNHLNLFKGHKKAVSYVKFLNSESIVSASTDSQLKLWNVNRSDCMRSFTGHTNEKNFVGLATDGEYITCGSENNDLHVYYKGLPKSLFSFKFDSVKTFLDRNNRE-DNANEFVSAVCWRQGTNVVLCANSQGVIKVLELTSGP 576          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000002459 (supercontig:LSalAtl2s:LSalAtl2s1472:24513:26207:-1 gene:EMLSAG00000002459 transcript:EMLSAT00000002459 description:"maker-LSalAtl2s1472-snap-gene-0.16")

HSP 1 Score: 81.2629 bits (199), Expect = 4.843e-16
Identity = 58/167 (34.73%), Postives = 81/167 (48.50%), Query Frame = 0

Query:  374 VSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVE-------HEKRCWSVDFNSVDTKLFASASDDARVKLWST-SVNHSIASLEAKANVCSVK---FNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRF--LNATELLSASTDSQLKLWDVEK 527
            +SWN      LLS+  D T+ LWD     K    ++       H      V ++ +   LF S +DD ++ +W T S  H+  S    A+   V    FNP S F +A GSAD  V  +DLRNLK  L  F  HK  +  V++   N T L S+ TD +L +WD+ K
Sbjct:  279 LSWNPNLNGYLLSASDDHTICLWDINAQPKDNKVIDAKTIFTGHTAVVEDVAWHLLHEXLFGSVADDQKLMIWDTRSATHNKPSHTVDAHAAEVNCLSFNPYSEFILASGSADKTVALWDLRNLKLKLHSFESHKDEIFQVQWSPHNETILASSGTDRRLHVWDLSK 445          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000001111 (supercontig:LSalAtl2s:LSalAtl2s1186:117586:119588:-1 gene:EMLSAG00000001111 transcript:EMLSAT00000001111 description:"maker-LSalAtl2s1186-snap-gene-1.40")

HSP 1 Score: 76.6406 bits (187), Expect = 9.207e-15
Identity = 68/252 (26.98%), Postives = 118/252 (46.83%), Query Frame = 0

Query:  322 VSSIEFDKDAEFFAIAGVTKRIKVY-DYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKAN-VCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFL-NATELLSASTDSQLKLWDVEKKTCIQSYQGHINEK-----NFVGLTSDEDYFAGGSENNSFFLF 565
            VS+++F  + E+ A +   K IKV+  Y+      +  H           IS V+W+S     L+S+  D T+ +W+  + +  K+   H    +  +FN   + L  S S D  V++W       + +L A ++ V +V FN      V+  S D     +D  + +   T+       VS+V+F  N   +L+A+ D+ LKLW+  K  C+++Y GH NEK     NF    +   +   GSE+N  +++
Sbjct:   33 VSAVKFSPNGEWLASSSADKLIKVWGSYDGKFEKTISGH--------KLGISDVAWSS-DSRLLVSASDDKTLKIWELSSGKCLKTLKGHSNYVFCCNFNP-QSNLVVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNRDGSLIVS-SSYDGXCRIWDTASGQCLKTLIDNDNPPVSFVKFSPNGKYILAATLDNTLKLWEYSKGKCLKTYSGHRNEKYCVFANFS--VTGGKWIVSGSEDNMVYIW 271          

HSP 2 Score: 59.6918 bits (143), Expect = 2.231e-9
Identity = 47/176 (26.70%), Postives = 84/176 (47.73%), Query Frame = 0

Query:  371 ISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKAN--VCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLN-----ATELLSASTDSQLKLWDVEKKTCIQSYQGHIN 539
            + C ++N    N ++S  +D +V +WD RT +  K+   H     +V FN  D  L  S+S D   ++W T+    + +L    N  V  VKF+P   + +A  + D+ +  ++    K  L  + GH+    Y  F N        ++S S D+ + +W+++ K  +Q  QGH +
Sbjct:  117 VFCCNFNP-QSNLVVSGSFDESVRIWDVRTGKCLKTLPAHSDPVSAVHFNR-DGSLIVSSSYDGXCRIWDTASGQCLKTLIDNDNPPVSFVKFSPNGKYILA-ATLDNTLKLWEYSKGKC-LKTYSGHRNE-KYCVFANFSVTGGKWIVSGSEDNMVYIWNLQTKEIVQKLQGHTD 287          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000006156 (supercontig:LSalAtl2s:LSalAtl2s336:420428:421924:-1 gene:EMLSAG00000006156 transcript:EMLSAT00000006156 description:"augustus_masked-LSalAtl2s336-processed-gene-4.9")

HSP 1 Score: 66.2402 bits (160), Expect = 2.304e-11
Identity = 57/206 (27.67%), Postives = 90/206 (43.69%), Query Frame = 0

Query:  374 VSWNSFHKNQ--LLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFN--------SVDTKLFASASDDARVKLWSTSVNHSIASLEAK-ANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFH--GHKKAVSYVRFLNATEL-LSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLF 565
            +SW +F  N   LL+  + G   LW     ++ ++   H     S+ FN        S      AS S D  VKLW+   +  IA +E   + V    ++P+  F +A    D+    +DL  L+    V H  GH K V  + F N   L L+   DS  ++WD+    CI   +GH+     V  + D  + A GS +N+  ++
Sbjct:  207 ISWCTFSPNSQYLLTGSWSGLCKLWSVPDCKEIRTLRGHSSNIGSIVFNPHATLEGHSNKDPCMASCSQDGSVKLWNLESDEPIADIEGHDSRVSRCAYHPSGRF-LATCVYDNSWRLWDLEQLQE---VLHQEGHSKPVHCIAFQNDGSLALTGGLDSFGRIWDLRTGQCIMFLEGHLKGIIGVDWSRDGYHCATGSSDNACKIW 408          

HSP 2 Score: 54.6842 bits (130), Expect = 9.171e-8
Identity = 50/186 (26.88%), Postives = 78/186 (41.94%), Query Frame = 0

Query:  389 YDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAK-ANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPL 573
            YD +  LWD    Q+      H K    + F + D  L  +   D+  ++W       I  LE     +  V ++    +H A GS+D+    +DLR  K   T+   H   VS V F +   LL+AS D   KLW  +    + S +GH        ++ D  Y A  S + +F L+   + + L
Sbjct:  317 YDNSWRLWDLEQLQEVLHQEGHSKPVHCIAFQN-DGSLALTGGLDSFGRIWDLRTGQCIMFLEGHLKGIIGVDWSRDG-YHCATGSSDNACKIWDLRKRKIEYTI-PAHTNVVSNVVF-DEDYLLTASYDGTAKLWARKTWQPLASLKGHDLRLMDCAISPDRSYIATCSYDRTFKLWSXDIKQRL 498          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000009105 (supercontig:LSalAtl2s:LSalAtl2s573:267932:269786:1 gene:EMLSAG00000009105 transcript:EMLSAT00000009105 description:"maker-LSalAtl2s573-augustus-gene-2.14")

HSP 1 Score: 65.0846 bits (157), Expect = 2.960e-11
Identity = 56/203 (27.59%), Postives = 97/203 (47.78%), Query Frame = 0

Query:  374 VSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKAN-VCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFL---------NAT-ELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLF 565
            V WN    N +LS+  D T  +WD++T    + +  H      VD+ + ++  FAS S D  + +    V   I S     N V S+K++P   F +A GS D  +  + + + +S +     H KA+  +++          NA   L+SAS DS ++LWDVE+ +C+ +   H    + V  + D  + A GS +   +++
Sbjct:  121 VKWNK-SGNYILSASEDRTTIIWDSQTGNHNQQFAFHSAPILDVDWQTDES--FASCSKDKCIHVCKLGVEKPIKSFYGHTNEVHSIKWDPQGKF-LASGSNDTTLKVWCM-DKESHVHDLKAHSKAIYTIKWSCTGPGTNNPNADLVLVSASYDSTVRLWDVERGSCLHTLTRHQETVSSVAFSPDGKFLASGSYDKFVYIW 318          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000005738 (supercontig:LSalAtl2s:LSalAtl2s309:736085:739351:-1 gene:EMLSAG00000005738 transcript:EMLSAT00000005738 description:"maker-LSalAtl2s309-augustus-gene-7.97")

HSP 1 Score: 65.855 bits (159), Expect = 4.070e-11
Identity = 59/238 (24.79%), Postives = 111/238 (46.64%), Query Frame = 0

Query:  303 IRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLS-SDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSV-NHSIASL-EAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSA----STDSQLKLWDVEKKTCIQS 533
            ++V+   N ++D Y     +  +++  D     I   + R+ +++ ND   D +  H P       S+ +CV+W    +N+ L+ +DY G +TLWDA   +  +    H  R  ++ ++  +    AS S    V +    + NH I +L E    VC + +N      +A G  D+ V  +D+R+L  P  V   H+ AV  +++   + +  A    ++D  LK+W+     C++S
Sbjct:  534 VKVLDAPNLSNDFY-----IRVMDWSSDNILSVI--FSSRVLLWNANDTSVDNLTEH-PEG-----SEYTCVAW----ENKYLAIADYSGHITLWDASVKKCLRRLSGHFNRVGTLGWSEHN---LASGSRSGEVLIHDVRIKNHIICTLNEHTQEVCGIDWNSGGTL-LATGGNDNTVFIWDIRSLDKPTKVLTQHQXAVKGIKWCPWSNITLATGGGTSDRCLKIWNASNGNCVKS 750          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000008384 (supercontig:LSalAtl2s:LSalAtl2s512:319189:321924:1 gene:EMLSAG00000008384 transcript:EMLSAT00000008384 description:"maker-LSalAtl2s512-augustus-gene-3.19")

HSP 1 Score: 63.1586 bits (152), Expect = 1.033e-10
Identity = 54/199 (27.14%), Postives = 95/199 (47.74%), Query Frame = 0

Query:  371 ISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLE--AKANVCSVKFNPT-SCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFL-NATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLF 565
            +  V +NS     L +SD D ++ LW     +  KS+  H     S  + S + K  AS SDD  +KL+  +    I S     +     + F+P+ SC  +A G++D  V  +D+R +K   + +  H   V+ V F  +   LLS S+D  +K++D+ +   +    GH  E   +  +   +YF+ G  +N+ F++
Sbjct:   33 VRSVDFNSDGSKXLSASD-DKSIKLWTVEKQRFIKSFNGHNNWVRSAKW-SXBQKXIASVSDDRSLKLFDENTGKEIHSFNEPGRGFGQCLSFHPSGSC--IAIGTSDKKVKMYDIRTMKLHQS-YTSHTGPVNQVAFHPSGCYLLSCSSDGFVKMFDIIEAQPVYGLTGHKEEVTAIEFSPKGEYFSSGGSDNAVFVW 226          

HSP 2 Score: 52.7582 bits (125), Expect = 2.407e-7
Identity = 32/95 (33.68%), Postives = 51/95 (53.68%), Query Frame = 0

Query:  483 LRNLKSPLTVFHGHKKAVSYVRF-LNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSF 576
            ++++K   T F GH+  V  V F  + ++ LSAS D  +KLW VEK+  I+S+ GH N       + ++   A  S++ S  LF +   K + SF
Sbjct:   16 VKSVKGQXTDFKGHQGPVRSVDFNSDGSKXLSASDDKSIKLWTVEKQRFIKSFNGHNNWVRSAKWSXBQKXIASVSDDRSLKLFDENTGKEIHSF 110          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000003862 (supercontig:LSalAtl2s:LSalAtl2s209:625539:633615:-1 gene:EMLSAG00000003862 transcript:EMLSAT00000003862 description:"maker-LSalAtl2s209-snap-gene-5.15")

HSP 1 Score: 62.7734 bits (151), Expect = 3.095e-10
Identity = 54/181 (29.83%), Postives = 81/181 (44.75%), Query Frame = 0

Query:  381 KNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAK-ANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRF-LNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSEN 559
            K+ LLS   DGT+ LW          Y  H    W V F S     FASA  D  V LWST  +HS+       ++V  + F+P +C ++A GS+D  V  +D  +  + + +  GHK AVS + F  +   L S   DS++  WD+     +     H      +  + D +  A  S +
Sbjct:  413 KSMLLSCSEDGTIRLW--------ICYRGHMYAVWHVKF-SPQGYYFASAGHDKTVMLWSTEQHHSLRIFAGHFSDVDCIAFHP-NCNYIASGSSDRSVRVWDCVS-GNCVRLMTGHKTAVSTLAFSPDGRFLASGGRDSRVLFWDIAHGHLLADLSNHSQTVTALAFSRDGNILAXSSTD 582          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000001730 (supercontig:LSalAtl2s:LSalAtl2s129:321159:324031:-1 gene:EMLSAG00000001730 transcript:EMLSAT00000001730 description:"maker-LSalAtl2s129-snap-gene-3.7")

HSP 1 Score: 62.3882 bits (150), Expect = 4.119e-10
Identity = 45/167 (26.95%), Postives = 76/167 (45.51%), Query Frame = 0

Query:  379 FHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKAN-VCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFV 544
             H+NQ++S   D T+ +WD  T +       H      V +   D +L  S + D  VK+W+      + +L    N V S++F+     HV  GS D  +  +D+        +  GH+   S +   N   L+S + DS +K+WD+    C+Q+  GH   ++ V
Sbjct:  413 LHENQVVSGSRDSTLRIWDIETGECLHILAGHLAAVRCVQY---DGRLVVSGAYDYMVKVWNPEREECLHTLSGHTNRVYSLQFDG---IHVVSGSLDTSIRVWDVETGACKHALM-GHQSLTSGMELRNNI-LVSGNADSTVKVWDITNGQCLQTLSGHNKHQSAV 571          

HSP 2 Score: 58.9214 bits (141), Expect = 6.019e-9
Identity = 47/200 (23.50%), Postives = 86/200 (43.00%), Query Frame = 0

Query:  371 ISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFH-----VAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLF 565
            I+C+    F  N+++S   D T+ +W A T +  ++ V H    WS         +  S S D  +K+W+      + +L    +          C H     V  GS D  +  +D+   +  L +  GH  AV  V++ +   ++S + D  +K+W+ E++ C+ +  GH N      L  D  +   GS + S  ++
Sbjct:  328 ITCLQ---FSNNRIVSGSDDNTLKVWSATTGKCLRTLVGHTGGVWSXQMRD---NIIVSGSTDRSLKVWNADAGTCVHTLYGHTST-------VRCMHLHENQVVSGSRDSTLRIWDIETGEC-LHILAGHLAAVRCVQY-DGRLVVSGAYDYMVKVWNPEREECLHTLSGHTNR--VYSLQFDGIHVVSGSLDTSIRVW 510          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. L. salmonis genes
Match: EMLSAG00000011537 (supercontig:LSalAtl2s:LSalAtl2s799:381305:384324:1 gene:EMLSAG00000011537 transcript:EMLSAT00000011537 description:"maker-LSalAtl2s799-snap-gene-3.77")

HSP 1 Score: 60.4622 bits (145), Expect = 1.280e-9
Identity = 63/266 (23.68%), Postives = 110/266 (41.35%), Query Frame = 0

Query:  371 ISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKAN-VCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFL---------NATELL-SASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
            I  + WN    N +LS+  D T  +WD++T    + +  H      VD+ + ++  FAS S D  + +    V   I S     N V ++K++P   F +A  S D  +  + + + +S +     H K +  +++          NA  +L SAS DS ++LWDVE+ +C+     H      V  + D  + A GS +    ++     K + S+K                     +  +CW    D +  + S G V +  L
Sbjct:  236 IFALKWNK-SGNYILSAGVDRTTIIWDSQTGNHKQQFAFHSAPALDVDWQTDES--FASCSTDKCIHVCKLGVEKPIKSFYGHTNEVNAIKWDPQGKF-LASCSDDMTLKVWCM-DKESHVHDLQAHSKEIYTIKWSCTGPGTNNPNADLVLASASFDSTVRLWDVERGSCLHXLXRHKEPVYSVAFSPDGKFLASGSFDKCVHIWSTVTGKLVHSYKGTGG-----------------IFEVCWNSRGDKVGASASDGTVFVLDL 479          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|75329161|sp|Q8H3I4.2|GLO4_ORYSJ (RecName: Full=Peroxisomal (S)-2-hydroxy-acid oxidase GLO4; AltName: Full=Glycolate oxidase 4; Short=GOX 4; Short=OsGLO4; AltName: Full=Short chain alpha-hydroxy acid oxidase GLO4)

HSP 1 Score: 300.056 bits (767), Expect = 9.932e-92
Identity = 155/347 (44.67%), Postives = 216/347 (62.25%), Query Frame = 0

Query:  679 AHKVLPRNALDYYRSGADQMQTLHDNQKAFQRFRIAPKMLVDVSRRDLSNSVLGRSVNFPIGIAPTAMQRMAHPEGECASARAAQEMGTIFILSTIATSSIEEVAQAAPRARKWFQLYIYKDREVTKDLVLRAEKAGYEALVLTVDAPFFGRRLADIRNKFQLPAHLNMANFVGLGDLATKAGTSGGGSGINNYVASLFDQTLSWKDVAWLKSFSKLPIILKGILRPDDALTGLKYGASAIIVSNHGARQLDGVLATIDVLSSIKAVVGNRCELYLDGGVRLGTDVLKALALGAKMVFLGRPVIWGLAYDGQKGVKKTLSLIRDELDSAMALSGCPSVSNVDRDLVI 1025
            A K LP+ A DY   GA+   TL +N  A+ R  + P++LVDVS+ D+S ++LG ++  PI +APT   ++AHPEGE A+ARAA     I +LS  ++  IE+VA +   A +++QLY+YK+R V+  LV RAE  G++AL+LTVD P  GRR ADIRNK   P   N+   +   D  T       GS +  +  +  D +LSWKD+ WLKS + +PI LKGI+  +DA   ++ G + +IVSNHGARQLD   ATI  L  +   V     + +DGG+R GTDV KALALGA+ V +GRPV +GLA  G+ G +  + ++  EL+ AMAL GC SV  + R  V+
Sbjct:   16 AKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMSTTLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSC-NAIRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEGLMTTDDHDTT-----NGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEAGVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARAVMVGRPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVM 356          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|75337885|sp|Q9SYX2.1|SPA1_ARATH (RecName: Full=Protein SUPPRESSOR OF PHYA-105 1)

HSP 1 Score: 317.39 bits (812), Expect = 2.848e-91
Identity = 179/488 (36.68%), Postives = 275/488 (56.35%), Query Frame = 0

Query:  188 LLTEFLNVLKKEKQEALAKAKQDLDMVHSDL-ESTLKHAHRLGVDPEVSSDSALRKE---SPL-------------SARMQSMHQHYPNLVDCYFETRRSV----------ASEPLGDSPR---------------PNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKT--------CIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
            LL  FL+ L+ +K++  +K  QD+  +  D+ E+  +++  + +   V S  A+ K    SPL             +A    +  +   L D YF  R  +          + + L D  R                +   L  F   L KF +YS       +  + D+ ++AS+V S+ FD D E  A AG++K+IK++D+N  + + V +HYP  EM++ SK+SCV WNS+ KN L S+DYDG V +WDA T Q    Y EH+KR WSVDF+  D   F S SDD  VKLWS +   S+ ++ + ANVC V+F+  S   +AFGSAD+ V+ +DLR +K+P     GH+KAVSYV+F+++  ++SASTD+ LKLW++ K          C  +Y+GH N+KNFVGL+  + Y A GSE N  + +YK L  P+ S+KF +   + G  ++Y D++ +FVS++CWRK ++ ++ ANS G +K+ +L
Sbjct:  547 LLLHFLSSLEVQKKKKASKLLQDIQTLEDDIKEAERRYSSNVSL---VRSHGAIEKRVQSSPLDEHCTTSSALFVPTANTDRLMSNIRQLEDAYFFMRSQINLSSSAATARSDKTLKDRDRCSENQNENQDMSTKGKSSDQLEVFFEGLCKFARYSKFETCGTIR-SGDLLNSASVVCSLSFDPDEEHIAAAGISKKIKIFDFNAFMNESVGVHYPLVEMVNKSKLSCVCWNSYIKNYLASTDYDGVVQIWDAGTGQGFSQYTEHQKRAWSVDFSPSDPTKFVSGSDDCSVKLWSINEKRSLGTIWSPANVCCVQFSSYSNHLLAFGSADYKVYCYDLRYVKTPWCTLAGHEKAVSYVKFMDSETIVSASTDNSLKLWNLNKTNSSGLSPGACSLTYKGHTNQKNFVGLSVLDGYIACGSETNEVYSYYKSLPMPMTSYKFGSVDPISG--NEYFDDNGQFVSSVCWRKKSNMLVAANSTGNMKLLKL 1028          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|75332075|sp|Q94BM7.1|SPA4_ARATH (RecName: Full=Protein SPA1-RELATED 4)

HSP 1 Score: 310.842 bits (795), Expect = 1.092e-90
Identity = 152/360 (42.22%), Postives = 234/360 (65.00%), Query Frame = 0

Query:  272 ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVE------KKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
            + +P+ DS +   G ++ F   L K+  +S +RV A+L    D+ +++++V +I FD+D EFFA AGV K+IK+++   +I+D  DIHYP  E+ S SK+S + WNS+ K+Q+ SS+++G V +WD    Q      EHEKR WS+D++S D  L AS SDD  VKLWS +   SI +++ KAN+C V+F   +   +AFGSADH V+Y+DLRN K PL    GH K VSYVRF++++ L+S+STD+ LKLWD+        +T + S+ GH N KNFVGL+  + Y A GSE N  F+++K    P+ S+KF   +T+   ++  +D++++F+S++CWR  +  ++ ANS G +KI  +
Sbjct:  440 SKDPINDSRQG--GWIDPFLEGLCKYLSFSKLRVKADLK-QGDLLNSSNLVCAIGFDRDGEFFATAGVNKKIKIFECESIIKDGRDIHYPVVELASRSKLSGICWNSYIKSQVASSNFEGVVQVWDVARNQLVTEMKEHEKRVWSIDYSSADPTLLASGSDDGSVKLWSINQGVSIGTIKTKANICCVQFPSETGRSLAFGSADHKVYYYDLRNPKLPLCTMIGHHKTVSYVRFVDSSTLVSSSTDNTLKLWDLSMSISGINETPLHSFMGHTNVKNFVGLSVSDGYIATGSETNEVFVYHKAFPMPVLSYKF---KTIDPVSELEVDDASQFISSVCWRGQSSTLVAANSTGNIKILEM 793          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|13124286|sp|Q9NYQ2.1|HAOX2_MOUSE (RecName: Full=Hydroxyacid oxidase 2; Short=HAOX2; AltName: Full=(S)-2-hydroxy-acid oxidase, peroxisomal; AltName: Full=Medium chain alpha-hydroxy acid oxidase; AltName: Full=Medium-chain L-2-hydroxy acid oxidase)

HSP 1 Score: 296.59 bits (758), Expect = 1.577e-90
Identity = 151/357 (42.30%), Postives = 225/357 (63.03%), Query Frame = 0

Query:  668 TMACLDDIEAHAHKVLPRNALDYYRSGADQMQTLHDNQKAFQRFRIAPKMLVDVSRRDLSNSVLGRSVNFPIGIAPTAMQRMAHPEGECASARAAQEMGTIFILSTIATSSIEEVAQAAPRARKWFQLYIYKDREVTKDLVLRAEKAGYEALVLTVDAPFFGRRLADIRNKFQLPAHLNMANFVGLGDLATKAGTSGGGSGINNYVASLFDQTLSWKDVAWLKSFSKLPIILKGILRPDDALTGLKYGASAIIVSNHGARQLDGVLATIDVLSSIKAVVGNRCELYLDGGVRLGTDVLKALALGAKMVFLGRPVIWGLAYDGQKGVKKTLSLIRDELDSAMALSGCPSVSNVDRDLV 1024
            ++ CL D +A A K L + + D+    AD   T +DN  AF+R R+ P+ L DVS+ D   ++ G+ +N PI I+PTA   +A  +GE ++A+AAQ+    +++S+ A+ ++E++  AAP    WFQLY+  D ++ K +V R E  G++ALV+TVDAP  G R  + R+   L A++ + +    G+           SG+   + S+   +  W D+  L+S ++LPIILKGIL  +DA   +K+    IIVSNHG RQLD V A+ID L  + A V  + E+Y+DGGVR G DVLKALALGA+ +FLGRP+IWGLA  G+ GVK+ L ++++EL + MALSGC SV+ +  DL+
Sbjct:    2 SLLCLADFKAQAQKQLSKTSWDFIEGEADDGITYNDNLAAFRRIRLRPRYLRDVSKIDTRTTIQGQEINAPICISPTAFHSIAWADGEKSTAKAAQKANICYVISSYASYTVEDIVAAAPGGLHWFQLYVQPDWDINKQMVQRIEALGFKALVVTVDAPVLGNRRGNKRSLLDLEANIKLKDLRSPGE---------SKSGLPTPL-SMPSSSSCWNDLPLLQSMTRLPIILKGILTKEDAELAVKHNIRGIIVSNHGGRQLDEVPASIDALREVVAAVNGKIEVYMDGGVRTGNDVLKALALGARCIFLGRPIIWGLACKGEDGVKEVLDILKEELHTCMALSGCRSVAEISPDLI 348          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. SwissProt
Match: gi|317376202|sp|B8B8K5.2|GLO4_ORYSI (RecName: Full=Peroxisomal (S)-2-hydroxy-acid oxidase GLO4; AltName: Full=Glycolate oxidase 4; Short=GOX 4; Short=OsGLO4; AltName: Full=Short chain alpha-hydroxy acid oxidase GLO4)

HSP 1 Score: 294.664 bits (753), Expect = 9.979e-90
Identity = 153/347 (44.09%), Postives = 214/347 (61.67%), Query Frame = 0

Query:  679 AHKVLPRNALDYYRSGADQMQTLHDNQKAFQRFRIAPKMLVDVSRRDLSNSVLGRSVNFPIGIAPTAMQRMAHPEGECASARAAQEMGTIFILSTIATSSIEEVAQAAPRARKWFQLYIYKDREVTKDLVLRAEKAGYEALVLTVDAPFFGRRLADIRNKFQLPAHLNMANFVGLGDLATKAGTSGGGSGINNYVASLFDQTLSWKDVAWLKSFSKLPIILKGILRPDDALTGLKYGASAIIVSNHGARQLDGVLATIDVLSSIKAVVGNRCELYLDGGVRLGTDVLKALALGAKMVFLGRPVIWGLAYDGQKGVKKTLSLIRDELDSAMALSGCPSVSNVDRDLVI 1025
            A K LP+ A DY   GA+   TL +N  A+ R  + P++LVDVS+ D+S ++LG ++  PI +APT   ++AHPEGE A+ARAA     I +LS  ++  IE+VA +   A +++QLY+YK+R V+  LV RAE  G++AL+LTVD P  GRR ADIRNK   P   N+   + + D  T       GS +  +  +  D +LSWKD+ WLKS + +PI LKGI+  +DA   ++ G + +IVSNHGARQLD   ATI  L  +   V     + +DGG+R GTDV KALALGA+ V    PV +GLA  G+ G +  + ++  EL+ AMAL GC SV  + R  V+
Sbjct:   16 AKKALPKMAYDYINGGAEDEHTLRENIAAYTRIILRPRVLVDVSKIDMSTTLLGYTMRSPIIVAPTGGHKLAHPEGEKATARAAASCNAIMVLSFSSSCKIEDVASSC-NAIRFYQLYVYKNRNVSATLVRRAESCGFKALLLTVDTPMLGRREADIRNKMVFPRSGNLEGLMTIDDHDTT-----NGSQLERFARATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEAGVAGVIVSNHGARQLDYAPATIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARAVMXXXPVFFGLAARGEAGARHVIEMLNGELEVAMALCGCRSVGEITRSHVM 356          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|1026572726|gb|OAD59875.1| (E3 ubiquitin-protein ligase RFWD2 [Eufriesea mexicana])

HSP 1 Score: 587.03 bits (1512), Expect = 0.000e+0
Identity = 303/622 (48.71%), Postives = 408/622 (65.59%), Query Frame = 0

Query:   41 NYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGAS-STPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE---STLKHAHRLGVDPE---------------------VSSDSALRKE-------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
            +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H+ AG   S P      P  GL   +   S ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E   + ++++ ++  D+E   + L+      +  E                     V SD+ +          S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  + ++A LNY+SD+Y+N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFD  R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:  119 DYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCGYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYTADGRHKVAGTDLSVP------PYDGLRDIIAAES-ANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMEEVENILRDVQNKSIRKEMIGLIDIIDSNMVKPNEKASVVGSDTFINPTGSKNAVGSTLAVRRKRMHAHFDDFVQCYFDSRAKELHLGHKSQSQSDSWQGTNSGLDVFRENLVKFSRYKSLNLLATLNYSSDIYNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHHKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDTVRSILEMQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 730          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|1101362169|ref|XP_018906743.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like isoform X1 [Bemisia tabaci])

HSP 1 Score: 583.948 bits (1504), Expect = 0.000e+0
Identity = 305/635 (48.03%), Postives = 413/635 (65.04%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACS-KVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDL---ESTLKHAHRLGVDPE--------------VSSDSALRKES-----------------PLSARMQSMHQHYPNLVDCYFETR----------RSVASEPLGDSPRPNPG---GLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRLG 626
             +YLCPICFE+ +EA +TKCGH+FC +C+  +LE  +++CPKC  +I     + PNF LN L  +                + R+  +  G S   S   L P+    +        NL+  D+  +   L Q ++++ E E+C+ +  LL EFL  L K K+  L +  +++ ++  D+   ES L      G+D E                SD+ +  +S                  LS R + MH H+ + V CYF  R          +S   E   D P  + G   GL+QF  +L KF+++SG+R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKVYDY  VIRD+VDIHYP  EM+S+SKISC+SWNS+HKN L SSDY+GTVT+WD  T Q+T+++ EHEKRCWSVDFN V+ +L AS SDDARVKLWS  V+HS+ASLEAKANVC V+FNP S  H+AFGSADHCVHY+DLRN+K+PL +F GH+KAVSYV+FLN  +++SASTDSQLKLW++    C++S  GHINEKNFVGL ++EDY A GSENN+ +++YKGL+K LFSFKFDA R++  + D+  DE+NEFVSA+CWR+ ++ ++ ANSQG +KI  L 
Sbjct:   38 NDYLCPICFELIEEAHITKCGHTFCFQCISKSLE-GNNRCPKCNSMIGGREHIFPNFLLNELITKY-------------KTKVRNLKDLVGGSDKVSDDPLPPLSDNLRGFVASESQNLALADVNVILEVLNQ-RKSILEAESCAVQNKLLHEFLKHLLKHKEAQLNQLTKEVSLIKRDINEVESILSTIDVKGIDLEQGSFDQEDFPPSDKAKSDAGMSCDSVESFNGSQKNSNNLTIGSLSMRRKRMHAHFNDFVRCYFNLRAKELLFSKNDKSPVPEKDKDKPLGSSGPSSGLDQFRDNLVKFSRFSGLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVYDYGAVIRDIVDIHYPCIEMLSNSKISCLSWNSYHKNTLASSDYEGTVTVWDVNTGQRTRTFQEHEKRCWSVDFNKVNIRLIASGSDDARVKLWSLDVDHSVASLEAKANVCCVQFNPGSSNHLAFGSADHCVHYYDLRNMKAPLALFEGHRKAVSYVKFLNNEDIVSASTDSQLKLWNINTSYCLRSLVGHINEKNFVGLAANEDYIACGSENNALYVYYKGLTKQLFSFKFDAVRSVLEK-DKKDDEANEFVSAVCWRQLSNVVVAANSQGIIKILELA 656          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|915669485|gb|KOC71003.1| (E3 ubiquitin-protein ligase RFWD2 [Habropoda laboriosa])

HSP 1 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 304/627 (48.48%), Postives = 411/627 (65.55%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGAS-STPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE---STLKHAHRLGVDPE---------------------VSSDSAL-----RKE------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFE+  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H A G   S P      P  GL   V   S ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E   + ++++ ++  D+E   + L+      +  E                     V SD+ +     +K+      S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNFVGL +D DY A GSENN+ +++YKGL+K LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:   50 NDYLCPICFEIIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYAADGRHRAVGTDLSVP------PYDGLRDIVAAES-ANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMEEVENILRDVQNKSIRKEMIGLIDIIDSNMVKPNEKASAVGSDTFVNPTGSKKQNDYAVGSTLAVRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATGQRTKAFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNLHCLRSFVGHVNEKNFVGLATDGDYVACGSENNALYIYYKGLTKQLFSYKFDAVRSILEIPERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 666          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|1059211601|ref|XP_017752844.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like [Eufriesea mexicana])

HSP 1 Score: 581.252 bits (1497), Expect = 0.000e+0
Identity = 304/642 (47.35%), Postives = 411/642 (64.02%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGAS-STPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE---STLKHAH---------RLGVDPEVSSDSALRKE--------------------------------------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H+ AG   S P      P  GL   +   S ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E   + ++++ ++  D+E   + L+            +   + + +  SA+RKE                                      S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  + ++A LNY+SD+Y+N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFD  R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:   68 NDYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCGYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYTADGRHKVAGTDLSVP------PYDGLRDIIAAES-ANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMEEVENILRDVQNKCPKVEDLKKSSENDTAQVSAIRKEMIGLIDIIDSNMVKPNEKASVVGSDTFINPTGSKNAVGSTLAVRRKRMHAHFDDFVQCYFDSRAKELHLGHKSQSQSDSWQGTNSGLDVFRENLVKFSRYKSLNLLATLNYSSDIYNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHHKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDTVRSILEMQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 699          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|925675420|gb|KOX72753.1| (E3 ubiquitin-protein ligase RFWD2 [Melipona quadrifasciata])

HSP 1 Score: 580.867 bits (1496), Expect = 0.000e+0
Identity = 300/625 (48.00%), Postives = 409/625 (65.44%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE---STLKHAHRLGVDPE--------------------VSSDSAL-----RKE------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFE+  EA +T+CGH+FC  C+V +LE  + +CPKC  ++ +  ++ PNF L+ L  +     K GL  L SS  A   H+       P      P  GL   +   S ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E   + ++++ ++  D+E   + LK      +  E                    V SD+ +     +K+      S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+G VT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:  118 NDYLCPICFEIIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYML-TQQDIFPNFLLHELISKYKTRIK-GLTELGSSYAADGRHKIGIDLPVP------PHDGLRDIIAAES-ANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKKDMEEVENILKDVQNKSIRKEMIGLIDIIDSNMVKPNEKANVGSDTFINPTGSKKQNDYAVGSTLAVRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKALRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGIVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDAVRSILEIQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 732          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|970889350|ref|XP_015109882.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like [Diachasma alloeum])

HSP 1 Score: 579.326 bits (1492), Expect = 0.000e+0
Identity = 302/630 (47.94%), Postives = 407/630 (64.60%), Query Frame = 0

Query:   41 NYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEALAKAKQDLDMVHSDLE---STLKHAH---------RLGVDPEVSSDSALRKE---------------------------SPLSARMQSMHQHYPNLVDCYFETRRS-----VASEPLG-DSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
            +YLCPICFEV  EA +T+CGH+FC +C+  TLE +  +CPKC   +    ++ PNF LN L  +     K G+     +       E+   SS P      P  GL   V   S ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E   + ++++ ++  D+E   S LK            +   + E +  SA+RKE                           S L+ R + MH H+ + V CYF+ R       V   P+  D+      GL+ F  +L KF++Y+ +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY  VIRD VDIHYP  EM+S SKISCVSWNS+HK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDT+L AS SDDARVKLWS +++HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNFVGL +D DY A GSENN+ +++YKGL+K LFS+KFDA RT+   +++  ++ NEFVSA+CWR+ ++ ++ ANSQG +KI  L
Sbjct:   42 DYLCPICFEVIDEAHITRCGHTFCYKCITKTLE-SLGRCPKCNFTLGQ-QDIFPNFLLNELVSKYKTRIK-GIGEFGCAHGDGRRRESGSESSVP------PCDGLRDFVAAES-ANLTLPDVNVMLEVLTQRKLLLEAESCAAQNKLLHEFLKHLLQQKEEQRNQLQKEVALIKRDMEEVESILKDVQSKCPRIEDLQRTRENETAQMSAIRKEMIDLIDIIDSNMVKHDENLQKPEHTTVSTLAVRRKRMHAHFEDFVQCYFDARAKDLLLGVPKNPVNIDTVHGMSSGLDVFRDNLVKFSRYNSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYGAVIRDTVDIHYPCIEMVSSSKISCVSWNSYHKGMLASSDYEGTVTVWDAATGQRTKAFQEHEKRCWSVDFNDVDTRLIASGSDDARVKLWSLNIDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKVWNINNPHCLRSFVGHVNEKNFVGLATDGDYVACGSENNALYVYYKGLTKQLFSYKFDAVRTVLELHERREEDPNEFVSAVCWRQMSNVVVAANSQGIIKILEL 661          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|1241833515|gb|PBC29833.1| (E3 ubiquitin-protein ligase [Apis cerana cerana])

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 305/645 (47.29%), Postives = 409/645 (63.41%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEA-------LAKAKQDLDMVHSDLESTLKHAHRLGVDPEVSSD------SALRKE-----------------------------------------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K G A L SS  A   H+           L +P H   + +     ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E        +A  K+D+D V + L        ++  D + SS+      SA+RKE                                         S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:  125 NDYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYTL-TQQDIFPNFLLHELISKYKTRIK-GFAELGSSYAADGKHKLGNTD------LSVPPHDGLRDIIAAESANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMDEVENILRDVQNKCPKVE-DLKKSSENDTAHVSAIRKEMIGLIDIIDSNMVKPNEKANISSDTFINPTGSKKQNDYAIGSTLATRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDAVRSILEIQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 759          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|820855890|ref|XP_012345148.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like [Apis florea])

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 306/646 (47.37%), Postives = 410/646 (63.47%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEA-------LAKAKQDLDMVHSDLESTLKHAHRLGVDPEVSSD------SALRKE------------------------------------------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H+           L +P H   + +     ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E        +A  K+D+D V + L        ++  D + SS+      SA+RKE                                          S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:   69 NDYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYAADGKHKLGNTD------LSVPPHDGLRDIIAAESANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMDEVENILRDVQNKCPKVE-DLKKSSENDTAHVSAIRKEMIGLIDIIDSNMVKPNEKANVISSDTFINPTGSKKQNDYAIGSTLATRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDAVRSILEIQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 704          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|572262111|ref|XP_006609321.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like [Apis dorsata])

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 306/646 (47.37%), Postives = 410/646 (63.47%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEA-------LAKAKQDLDMVHSDLESTLKHAHRLGVDPEVSSD------SALRKE------------------------------------------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H+           L +P H   + +     ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E        +A  K+D+D V + L        ++  D + SS+      SA+RKE                                          S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:   69 NDYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYAADGKHKLGNTD------LSVPPHDGLRDIIAAESANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMDEVENILRDVQNKCPKVE-DLKKSSENDTAHVSAIRKEMIGLIDIIDSNMVKPNEKANIISSDTFINPTGSKKQNDYAIGSTLATRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDAVRSILEIQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 704          

BLAST of e3 ubiquitin-protein ligase rfwd2-like vs. nr
Match: gi|1032012470|ref|XP_016772257.1| (PREDICTED: E3 ubiquitin-protein ligase RFWD2-like [Apis mellifera])

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 306/646 (47.37%), Postives = 410/646 (63.47%), Query Frame = 0

Query:   40 GNYLCPICFEVFKEAVVTKCGHSFCQECLVDTLEHTSSKCPKCYQVINSATEVSPNFALNALAEQVLNWQKSGLATLLSSAQARSSHEAAGASSTPSGSLLLPVHGLPQCVDYLSQANLSGKDLEDLSGWLGQHKRAVAEREACSKVILLTEFLNVLKKEKQEA-------LAKAKQDLDMVHSDLESTLKHAHRLGVDPEVSSD------SALRKE------------------------------------------SPLSARMQSMHQHYPNLVDCYFETRRSV-----ASEPLGDSPRPNPGGLNQFSTSLSKFTKYSGIRVMANLNYTSDMYSNASIVSSIEFDKDAEFFAIAGVTKRIKVYDYNDVIRDMVDIHYPRAEMISHSKISCVSWNSFHKNQLLSSDYDGTVTLWDARTTQKTKSYVEHEKRCWSVDFNSVDTKLFASASDDARVKLWSTSVNHSIASLEAKANVCSVKFNPTSCFHVAFGSADHCVHYFDLRNLKSPLTVFHGHKKAVSYVRFLNATELLSASTDSQLKLWDVEKKTCIQSYQGHINEKNFVGLTSDEDYFAGGSENNSFFLFYKGLSKPLFSFKFDAARTLFGRNDQYLDESNEFVSALCWRKGTDNIIGANSQGCVKIYRL 625
             +YLCPICFEV  EA +T+CGH+FC  C+V +LE  + +CPKC   + +  ++ PNF L+ L  +     K GLA L SS  A   H+           L +P H   + +     ANL+  D+  +   L Q K  +      ++  LL EFL  L ++K+E        +A  K+D+D V + L        ++  D + SS+      SA+RKE                                          S L+ R + MH H+ + V CYF++R         S+   DS +    GL+ F  +L KF++Y  +R +A LNY+SD+++N++IVSSIEFDKD EFFAIAGVTKRIKV+DY+ VIRD VDIHYP  EM+S SKISCVSWNSFHK  L SSDY+GTVT+WDA T Q+TK++ EHEKRCWSVDFN VDTKL AS SDDARVKLWS + +HS+ASLEAKANVC VKFNP S  H+AFGSADHCVHY+DLRN+K  L +F GH+KAVSYV+F+N  E++SASTDSQLK+W++    C++S+ GH+NEKNF+GLT+D DY A GSENN+ +++YKGLSK LFS+KFDA R++    ++  ++ NEFVSA+CW++ ++ ++ ANSQG +KI  L
Sbjct:   69 NDYLCPICFEVIDEAHITRCGHTFCYRCIVKSLE-ANGRCPKCSYTL-TQQDIFPNFLLHELISKYKTRIK-GLAELGSSYAADGKHKLGNTD------LSVPPHDGLRDIIAAESANLTLPDVNVMLEVLTQRKHLLEAETCAAQNKLLHEFLKHLLQQKEEQKNQLQKEIALIKRDMDEVENILRDVQNKCPKVE-DLKKSSENDTAHVSAIRKEMIGLIDIIDSNMVKPNEKANIISNDTFINPTGSKKQNDYAIGSTLATRRKRMHAHFDDFVQCYFDSRAKELLLGHKSQSQSDSWQGTSSGLDVFRENLVKFSRYKSLRPLATLNYSSDIFNNSTIVSSIEFDKDNEFFAIAGVTKRIKVFDYSAVIRDTVDIHYPCVEMVSSSKISCVSWNSFHKGMLASSDYEGTVTVWDAATCQRTKTFQEHEKRCWSVDFNDVDTKLIASGSDDARVKLWSLNNDHSVASLEAKANVCCVKFNPRSSCHLAFGSADHCVHYYDLRNMKEALCIFKGHRKAVSYVKFINKEEIVSASTDSQLKMWNINNPHCLRSFVGHVNEKNFIGLTTDGDYVACGSENNALYVYYKGLSKQLFSYKFDAVRSILEIQERREEDLNEFVSAVCWKQMSNVVVAANSQGTIKILEL 704