roundabout 1, maker-scaffold49_size462716-snap-gene-3.30 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000004040 (supercontig:LSalAtl2s:LSalAtl2s215:590464:702398:-1 gene:EMLSAG00000004040 transcript:EMLSAT00000004040 description:"maker-LSalAtl2s215-snap-gene-7.10") HSP 1 Score: 466.463 bits (1199), Expect = 5.608e-148 Identity = 285/750 (38.00%), Postives = 407/750 (54.27%), Query Frame = 0
Query: 277 DEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAV-KGRPHPEIRWSRRNGML-----PAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGF-VEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQT--PPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEALNFTLEPSSNSLILYNLTEGL--TYAVQVAAYNRQGVGPLCDPVELEVE--------------TDLFHPQLVPEKAP-GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
D G Y CVA+N G S ++ L + P + + G T C KG P P + W ++NG + +R++ D L + + +P DE Y C+A+N AG HL+V P P + G +++ CVA G P P++ W K GS L +G G+L I N D VY C + + +G A++ ++I + PPI + P NQ +G+ + C A P + W+K+G L +SDS LY CQA+S SGQ++ S+ L V+ ++F +P LS+FP SPS+P L NATS+ + + W KP R+GGSP++ Y Q++YYT +P HW + V SE T+ D +P TT+ VLVRA N +GLSPPSP+SK +T P DP +RSKL R+M L +GSRK+KL WELLI +FV GYHIQ+R + YD +TISGGGTRSHTL+DLRPNT+Y +F+LP+N RIKGRP+ L +V+TKEDVP+++P+ +KI+++N++ +IQWH PP RDL+GDL GFKVLI +N + N+TLEP NS ++ L+ Y +VAAYNRQGVGP V + + ++ F P+ E +P G D+V+QEVWFV VS +++L++ F A++C RR + + K+MGHYN
Sbjct: 105 DGGIYSCVARNTLG---SVESLLRDKFR----GEPVDSDVILGQTAELICXPPKGNPSPTVFW-KKNGQILDLETEEERLYIKDEGTLVIPDSIPGDEGSYQCIAKNIAGTKATQRIHLEVGIVPSITKAPENASIASGKEIRLVCVAEGKPKPMISWSKSAGSLPLSRSRIEG-------GLLIIKNAITTDEDVYRCMAENSAGMDTATAAIRIHS-----PPIFLVKPSNQKIAVGAPASLDCIAAGNPTPVLFWMKEG-----------------------LSLSDSGLYXCQARSVSGQSVWSSSLVVSEGGGLIDFKPMPDLSQFPGSPSKPSLANATSHSITVTWSKPHRVGGSPLKGY------------------------------QLDYYTLASPLHWTTVYNVH-SEFHTIDDLDPETTLWVLVRALNGYGLSPPSPVSKSLKTLAPDPNSRTGMDPWSIRSKLGG-RIMILIGAISVGSRKIKLEWELLISGEFVEGYHIQIRGSKE----PHYDSVTISGGGTRSHTLSDLRPNTKYFIFILPFNNRIKGRPSNLKVVSTKEDVPSSSPDRIKIRLVNVSTGLIQWHTPPSRDLNGDLSGFKVLIFVNSSLESNYTLEPDVNSFLITELSSDFETAYGAKVAAYNRQGVGPFSPMVSININPMSGDIHGVHRNELSEFFSPE---EASPHGFDIVLQEVWFVSFVSVMTLVLVIAFGAVICIRRRKGELKNMGHYN 772 HSP 2 Score: 194.897 bits (494), Expect = 3.909e-51 Identity = 124/332 (37.35%), Postives = 173/332 (52.11%), Query Frame = 0
Query: 20 PPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQK-----IQGNSPSEEQKEKEKLFEARKDEIGTES-----LTLSYCPKLDTRPQIELVVACNREDFDC-PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSR 340
PP+I HP+++ V +N+PVTLRC+ G P P+I W ++G TAA DH+S RVLLP+GSLFFLR V S+KE DGGIY C A NT G V S +L+DKFR EP D I G TA C PPKG+P+PT FWKK+G+IL+LE + +++ I + P +E + K+ GT++ L + P + P+ + + C +G M ++ + G P +R IEG G LII DE Y+C+A+N AG+ +A A + + P L P N G C G P P + W +
Sbjct: 32 PPLILSHPESTIVPKNDPVTLRCDTSGXPLPEIIWRRNGRKXTTAADDHRSRRVLLPNGSLFFLRTVHSRKETDGGIYSCVARNTLGSVES--------LLRDKFRGEPVDSDVILGQTAELICXPPKGNPSPTVFWKKNGQILDLETEEERLYIKDEGTLVIPDSIPGDEGSYQ----CIAKNIAGTKATQRIHLEVGIVPSITKAPENASIASGKEIRLVCVAEGKPKPMISWSKS---AGSLPLSRS--RIEG-GLLIIKNAITTDEDVYRCMAENSAGMD-TATAAIRIHSPPIFLVKPSNQKIAVGAPASLDCIAAGNPTPVLFWMK 344 HSP 3 Score: 178.333 bits (451), Expect = 6.997e-46 Identity = 89/216 (41.20%), Postives = 122/216 (56.48%), Query Frame = 0
Query: 260 LSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESG 475
L I+ EGTL+I P DEG YQC+AKN+AG + + + L + ++P + K PEN S +G +R C +G+P P I WS+ G LP R +G L + N + DEDVY C+AEN AG A+A +++ SPP+FLVKP++QK G+ + C+AAGNP P+LFWMKEG S DSG+Y C + S SG
Sbjct: 176 LYIKDEGTLVIPDSIPGDEGSYQCIAKNIAGTKATQRIHLEVGIVPSITKAPENASIASGKEIRLVCVAEGKPKPMISWSKSAGSLPLSRSRI-EGGLLIIKNAITTDEDVYRCMAENSAGMDTATAAIRIHSPPIFLVKPSNQKIAVGAPASLDCIAAGNPTPVLFWMKEG--------------------------LSLSDSGLYXCQARSVSG 364 HSP 4 Score: 54.6842 bits (130), Expect = 1.200e-7 Identity = 40/140 (28.57%), Postives = 63/140 (45.00%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDG 160
P IT+ P+N+S+ + + L C A+G P+P I W K +A R + G L A+ + D +Y C A N+ G + A + + F ++P + G A +C G+PTP FW K+G
Sbjct: 221 PSITKAPENASIASGKEIRLVCVAEGKPKPMISWSK-------SAGSLPLSRSRIEGGLLIIKNAITT----DEDVYRCMAENSAGMDTATAAIRIHS--PPIFLVKPSNQKIAVGAPASLDC-IAAGNPTPVLFWMKEG 346
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000005066 (supercontig:LSalAtl2s:LSalAtl2s266:205294:402426:1 gene:EMLSAG00000005066 transcript:EMLSAT00000005066 description:"maker-LSalAtl2s266-augustus-gene-3.8") HSP 1 Score: 405.601 bits (1041), Expect = 1.505e-123 Identity = 233/730 (31.92%), Postives = 386/730 (52.88%), Query Frame = 0
Query: 266 GTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVA-PAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQ--KFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEALN--FTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVE-TDLFHPQLV-PEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRR 986
G L+I +V DEGQ C A+N+ GIR + +A+LS+QV PY L P +++A++GD + C V G P P + WSR +G LP +V + D +L + ++ PED+ +Y C A+N G+VEAS + V + P KP DQ+ + C A GNP P LFW KEG ++ G+ G VS +G L I ++D G Y C+++S +G+ + +++ +D PPPII +G NQT + + PC+A+ P+ + W K+ PIS + RI V+ TL + L SD+ LYTC A S SG+ SA L P NV F P S FP P+ P +++ S + I W + G SP+ Y +EY++S T WV +SE +T+ + +P T+ LVRA N HG+SPP+ +S+ +T E+ + S +V++L ++ + S +++SW +L P+ +V G+ ++ RD+S + + + G +H +T+LR TEY VF++P+ +I+G+P+ + V T EDVP+A P ++ I+ +N T+A+I+W PP + +G L G+++ + NG+ + TL ++ S IL NLT Y+V+ A + G+GP P ++ T++ + + P + ++ E WF+ +++ V + F+ ++ +R+
Sbjct: 183 GNLVIQEVVKSDEGQXACQAENIVGIRETEEAQLSIQVKPYFLSKPLDITAISGDDIDIICDVSGDPDPIVIWSRDDGPLPENKV-SIDANRLQIRDVRPEDDGIYFCKAKNDVGYVEASFSISVHAKPAIQTKPKDQRIGMNGIAKFECSAGGNPPPSLFWTKEGSQELMFAGTKYGQRSVSEDGTLFIQGVRKEDEGFYICSALSVAGSTATKAYLEVTALRDTPPPIIIMGTANQTLPLNTIAILPCKASGAPKPTISWYKNQSPISIDDSSRILVESSGTLVIDNLISSDTGLYTCTASSESGETSWSASLSXXDPKNPNVIFHKTPDPSTFPDPPTRPKIVDRRSTSITISWRRSPNTGESPLMGY------------------------------TLEYFSSDLETGWVVAANRITSETYTINNLKPDTSYMFLVRAENSHGMSPPTRVSERVRTLQSLSENDGINIHEVSNALMSKVVQLTSIEAISSTTIRVSWSIL-PESMSYVEGFFVRYRDMSGGSQKFNMKTVMLETDGIVNHVITNLRKYTEYEVFLMPFYKKIEGQPSNSLHVQTMEDVPSAPPSDLLIEPMNSTSAIIRWSPPPPQHRNGVLLGYQIHVKGNGSTFHSNITTLNATTTSYILSNLTLHQKYSVRAVARTKIGLGPFSQPSFFVMDPTNIKYAIITHPNEGNINGNIMSEPWFIGLLTAVFVAISGTFIGIIIYRK 880 HSP 2 Score: 255.373 bits (651), Expect = 1.519e-70 Identity = 165/478 (34.52%), Postives = 243/478 (50.84%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE--------LDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLI---RLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRN-------GMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESG 475
P ITE P + +V +NEPVTL C+A G PEP I W+KDGE V TA K+HRV+LPSGSLFFLRAVQ+KKE+DGG+YWCEATN G RSRN +L VAVL++ FR+ P D +G + +C PPKG+P P+ W K+G IL+ + +Q++ + + + E + R+ E L++ P ++P ++ + D C + G+ +I G PE + +SI+ L I V+P+D+G Y C AKN G + A +S+ P + P++ +F C+ G P P + W++ G QR + DGT L + + EDE Y C A + AG A+L+V + PP+ ++ +Q + +PC A+G P P + W K + + + V G L I N D+G+YTC + SESG
Sbjct: 29 PQITEQPTSMTVPKNEPVTLNCKADGIPEPNITWYKDGELVPTAP---KTHRVILPSGSLFFLRAVQNKKEHDGGLYWCEATNEVGTARSRNVTLDVAVLREDFRLNPMDTIVAEGDIGVIKCWPPKGNPKPSVKWTKNGDILDTSQSNGRIIITEVGNLVIQEVVKSDEGQXACQAENIVGIRETE--EAQLSIQVKPYFLSKPLDITAISGDDIDIIC-----DVSGDPDPIVIWSRDDGPLPENK--VSIDA-NRLQIRDVRPEDDGIYFCKAKNDVGY-VEASFSISVHAKPAIQTKPKDQRIGMNGIAKFECSAGGNPPPSLFWTKEGSQELMFAGTKYGQRSVSEDGT-LFIQGVRKEDEGFYICSALSVAGSTATKAYLEVTALRDTPPPIIIMGTANQTLPLNTIAILPCKASGAPKPTISWYKNQSP---ISIDDSSRILVESSGTLVIDNLISSDTGLYTCTASSESG 488 HSP 3 Score: 111.694 bits (278), Expect = 4.651e-25 Identity = 87/317 (27.44%), Postives = 131/317 (41.32%), Query Frame = 0
Query: 277 DEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLK-HPENVSAVAGDTVRFGC-AVKGRPHPEIRWSRRNGMLPAQ----RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEA-SAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDG---VPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVA 581
D G Y C A N G S L + VL + +P + GD C KG P P ++W++ +L R+ T+ L + +V DE C AEN G E A L + P FL KP D + G + I C +G+P PI+ W ++ G LP + V + L+I + +D G+Y C + ++ G + AS + + P IQ P +Q + F C A P + W K+G + + + V D TL + + D Y C A S +G A+L V
Sbjct: 98 DGGLYWCEATNEVGTARSRNVTLDVAVLREDFRLNPMDTIVAEGDIGVIKCWPPKGNPKPSVKWTKNGDILDTSQSNGRIIITEVGNLVIQEVVKSDEGQXACQAENIVGIRETEEAQLSIQVKPYFLSKPLDITAISGDDIDIICDVSGDPDPIVIWSRDDGP---LP---ENKVSID-ANRLQIRDVRPEDDGIYFCKAKNDVGYVEASFSISVHAK-----PAIQTKPKDQRIGMNGIAKFECSAGGNPPPSLFWTKEGSQELMFAGTKYGQRSVSEDGTLFIQGVRKEDEGFYICSALSVAGSTATKAYLEVT 402 HSP 4 Score: 97.4413 bits (241), Expect = 9.714e-21 Identity = 93/389 (23.91%), Postives = 167/389 (42.93%), Query Frame = 0
Query: 26 HPQNSSVTRNEPVTLRC-EAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRIL---ELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIG----TESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNF-----GRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVL-----PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLP---AQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSP 393
+P ++ V + ++C +G+P+P +KW K+G+ + T+ ++ + ++ G+L V+S D G C+A N G + A L + V K F +P D+ AI G C G P P W +D L ++ +D + ++ ++ ++ F K+++G + S+++ P + T+P+ + + F+C G F ++L+ G + R S+ +GTL I V+ +DEG Y C A +VAG + KA L + L P ++ N + C G P P I W + + + R+ L + N++ D +YTC A + +G SA L P
Sbjct: 132 NPMDTIVAEGDIGVIKCWPPKGNPKPSVKWTKNGDILDTSQSNGRI--IITEVGNLVIQEVVKS----DEGQXACQAENIVGIRETEEAQLSIQV-KPYFLSKPLDITAISGDDIDIICDV-SGDPDPIVIWSRDDGPLPENKVSIDANRLQIRDVR-------PEDDGIYFCKAKNDVGYVEASFSISVHAKPAIQTKPKDQRIGMNGIAKFECSAGGNPPPSLFWTKEGSQELMFAGTKYGQR---SVSEDGTLFIQGVRKEDEGFYICSALSVAG-STATKAYLEVTALRDTPPPIIIMGTANQTLPLNTIAILPCKASGAPKPTISWYKNQSPISIDDSSRILVESSGTLVIDNLISSDTGLYTCTASSESGETSWSASLSXXDP 501 HSP 5 Score: 63.5438 bits (153), Expect = 2.562e-10 Identity = 72/295 (24.41%), Postives = 115/295 (38.98%), Query Frame = 0
Query: 305 PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLP----AQRVHTTDGTQLTL---MNIVPEDEDVYTCLAENRAGFVEA---SAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCV-AAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHD-NTLHFEELEISDSALYTCQAKSTSG--QAMLSAFLRVAPA 583
P + + P +++ + V C G P P I W + ++P RV G+ L N D +Y C A N G + + + V F + P D +G I C GNP P + W K G +L + G + ++ G L I + D G C + + G I + E ++ + P P++ T G + C+ + P V W +D P+ P KV D N L ++ D +Y C+AK+ G +A S + PA
Sbjct: 29 PQITEQPTSMTVPKNEPVTLNCKADGIPEPNITWYKDGELVPTAPKTHRVILPSGSLFFLRAVQNKKEHDGGLYWCEATNEVGTARSRNVTLDVAVLRED-FRLNPMDTIVAEGDIGVIKCWPPKGNPKPSVKWTKNG--DILDTSQSNGRIIITEVGNLVIQEVVKSDEGQXACQAENIVG-IRETEEAQLSI---QVKPYFLSKPLDITAISGDDIDIICDVSGDPDPIVIWSRDDGPL-----PENKVSIDANRLQIRDVRPEDDGIYFCKAKNDVGYVEASFSISVHAKPA 311 HSP 6 Score: 53.1434 bits (126), Expect = 3.727e-7 Identity = 49/188 (26.06%), Postives = 81/188 (43.09%), Query Frame = 0
Query: 391 FSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGML----RIGNTSEKDSGVYTCASVSESGAILASS---EVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQTRVEWLKDGVPIST-MNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSG 570
+ P +PT + V + C A G P P + W K+G L+P + + H + P G L + N E D G+Y C + +E G + + +V + R P+ I IG +P + P+ V+W K+G + T ++ RI + L +E+ SD CQA++ G
Sbjct: 26 YRSPQITEQPTSMTVPKNEPVTLNCKADGIPEPNITWYKDGE---LVPTAPKTHRVILPSGSLFFLRAVQNKKEHDGGLYWCEATNEVGTARSRNVTLDVAVLREDFRLNPMDTIVAEGD---IGVIKCWPPKGNPKPSVKWTKNGDILDTSQSNGRIIITEVGNLVIQEVVKSDEGQXACQAENIVG 207
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000004009 (supercontig:LSalAtl2s:LSalAtl2s214:573899:577288:-1 gene:EMLSAG00000004009 transcript:EMLSAT00000004009 description:"maker-LSalAtl2s214-augustus-gene-5.7") HSP 1 Score: 196.438 bits (498), Expect = 8.954e-52 Identity = 140/415 (33.73%), Postives = 193/415 (46.51%), Query Frame = 0
Query: 8 VLFSFPGCWAA-SPPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSP---SEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRP-------------EARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHP-ENVSA-VAGDTVRFGCAVKGRPHPEIRWSRRNG------MLPAQRVH----TTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSP 393
V F +A+ S P IT HP N++V R EP+TL C + G P PQI+WFK+G P+ T+ R++LP GSL FLR SKKE D G Y C ATN+ G S A+L +A +KD+F+ P+D A G + EC PP G P P WK+DG L+L I KI G QK E ++ I + ++ + ++ P PK + AI G R+G P + L + G+G L I V D G+Y C AKN AG +S+ + L++ + P + + P NV V+ D + C V G P P + WSR +LP + H DGT L + N YTC A N AG A +H+ V+ P
Sbjct: 8 VYFLLESVFASPSIPEITHHPLNTTVIRGEPLTLECRSSGFPPPQIRWFKNGSPLFTSPD-----RMILPDGSLLFLRVFHSKKETDSGDYHCTATNSQGSASSHIATLEIAYMKDEFKALPRDTEATLGRDIVLECSPPAGHPEPVVKWKRDGDHLDLTSSKRI----KIDGQGNLLIQSVQKSDEGRYQCSAGNIANKKMSKAVRLNVNAPPHF----------ISKPKDINAIAGEEAFIDCRVGGDPYPDVTWTKERGDIDISKLKIVSGKG-LRIKNVNSHDSGRYICTAKNRAG-XISSSSFLTISIPPVITERPLMNVQVRVSEDPIELHCGVSGSPSPFVYWSREADAEAGLLILPGEDKHGIIVKVDGT-LYIENPQTMHSGHYTCTAVNDAGSAIARSHVLVYDP 400 HSP 2 Score: 154.451 bits (389), Expect = 1.673e-38 Identity = 74/231 (32.03%), Postives = 130/231 (56.28%), Query Frame = 0
Query: 260 LSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKP--TDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSG---VLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKI 485
+ I+G+G L+I VQ DEG+YQC A N+A ++S RL++ P+ + P++++A+AG+ C V G P+P++ W++ G + ++ G L + N+ D Y C A+NRAG + +S+ L + PPV +P Q V +++ C +G+P P ++W +E + ++LPG ++ + V +G L I N SG YTC +V+++G+ +A S V +
Sbjct: 167 IKIDGQGNLLIQSVQKSDEGRYQCSAGNIANKKMSKAVRLNVNAPPHFISKPKDINAIAGEEAFIDCRVGGDPYPDVTWTKERGDIDISKLKIVSGKGLRIKNVNSHDSGRYICTAKNRAGXISSSSFLTISIPPVITERPLMNVQVRVSEDPIELHCGVSGSPSPFVYWSREADAEAGLLILPGEDKHGIIVKVDGTLYIENPQTMHSGHYTCTAVNDAGSAIARSHVLV 397 HSP 3 Score: 148.673 bits (374), Expect = 1.023e-36 Identity = 165/709 (23.27%), Postives = 297/709 (41.89%), Query Frame = 0
Query: 304 LPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGML--PAQRVHTTDGTQLTLMNIVPE---DEDVYTCLAENRAGFVEASAHLQV----FSPPVFLVKPTDQKGVQGSTVQIPCVA-AGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCE--ATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIR----IGGSPIQ-SYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTV----HVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEALNFTLEPSSN--SLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLF---HPQLVPEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRR 986
+P + HP N + + G+ + C G P P+IRW + L R+ DG+ L L + D Y C A N G AS+H+ + F P D + G + + C AG+P P++ W ++G L ++ + + +G L I + + D G Y C++ + + ++ + ++ + + PP I P + G C P V W K+ I ++K+ L + + DS Y C AK+ +G S+FL ++ P ++E P L+N Q+ + D PI + GSP Y RE L + + I+ + T ++ P+ S +T A + HVLV PI + AG ++ + R + + R+ ++ V S +K+ W + K++ G++I+ R + D + V + T S + +L ++EY VF+ PY I G+P + + T D+P+ AP + ++LN T + W+ + ++G L+G+++LI NGT ++N T++ +N +LI+ + Y +Q++ N G GP P LE+ F H +P D V+ W + +V+ + +L+ F++ V F +
Sbjct: 21 IPEITHHPLNTTVIRGEPLTLECRSSGFPPPQIRWFKNGSPLFTSPDRMILPDGSLLFLRVFHSKKETDSGDYHCTATNSQG--SASSHIATLEIAYMKDEFKALPRDTEATLGRDIVLECSPPAGHPEPVVKWKRDGDH---LDLTSSKRIKIDGQGNLLIQSVQKSDEGRYQCSAGNIANKKMSKA---VRLNVNAPPHFIS-KPKDINAIAGEEAFIDCRVGGDPYPDVTWTKERGDIDIS---KLKIVSGKGLRIKNVNSHDSGRYICTAKNRAGXISSSSFLTISIP---------PVITERP-------LMNV---QVRVSED-PIELHCGVSGSPSPFVYWSREADAEAGL-LILPGEDKHGIIVKVDG-----------TLYIENPQTMHSGHYTCTAVNDAGSAIARSHVLVY----------DPIHQDSFPDTNAGLELDEARIVTTLEEGVRISDISAV---SSTSLKVKWHITARHKYIDGFYIRYRKVEDPIVAT---VRVLRSDLTTSFVIDNLEEHSEYEVFIQPYFQNIVGKPASIKRIITHPDLPSEAPLIREARLLNSTTVFLSWNTISPQHINGPLRGYEILIKGNGT-SVNNTVDAETNEVTLIVNGMAPNKIYMIQISVINSIGHGPFSSP--LEISKGFFDEAHNPHLPIDHENKD--VKTTWIIAIVAVLTFVLI--FISGVFFYK 662 HSP 4 Score: 101.679 bits (252), Expect = 4.238e-22 Identity = 92/336 (27.38%), Postives = 140/336 (41.67%), Query Frame = 0
Query: 265 EGTLIISQV----QPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKH-PENVSAVAGDTVRFGCAV-KGRPHPEIRWSRRNGML---PAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGF-VEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQ-RIGSS-TNFPC--EATPQTRVEWLKD-----GVPISTMNDPR-IKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
+G+L+ +V + D G Y C A N G S A L + + K P + A G + C+ G P P ++W R L ++R+ L + ++ DE Y C A N A + + L V +PP F+ KP D + G I C G+P P + W KE G + + + LRI N + DSG Y C + + +G I +SS + I PP+I P+ Q R+ C +P V W ++ G+ I D I V D TL+ E + S YTC A + +G A+ + + V
Sbjct: 73 DGSLLFLRVFHSKKETDSGDYHCTATNSQGSASSHIATLEIAYMKDEFKALPRDTEATLGRDIVLECSPPAGHPEPVVKWKRDGDHLDLTSSKRIKIDGQGNLLIQSVQKSDEGRYQCSAGNIANKKMSKAVRLNVNAPPHFISKPKDINAIAGEEAFIDCRVGGDPYPDVTWTKERGD------IDISKLKIVSGKGLRIKNVNSHDSGRYICTAKNRAGXISSSSFLTISI-----PPVITERPLMNVQVRVSEDPIELHCGVSGSPSPFVYWSREADAEAGLLILPGEDKHGIIVKVDGTLYIENPQTMHSGHYTCTAVNDAGSAIARSHVLV 397 HSP 5 Score: 54.6842 bits (130), Expect = 1.228e-7 Identity = 48/195 (24.62%), Postives = 82/195 (42.05%), Query Frame = 0
Query: 392 SPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEG---MLRIGNTS-EKDSGVYTCASVSESGAILASSEV---KIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCE---ATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSA 576
S P P + ++G + + C ++G P P + W K G P + P+G LR+ ++ E DSG Y C + + G+ ASS + +I +D + P + +G C P+ V+W +DG + + RIK+D L + ++ SD Y C A + + + M A
Sbjct: 20 SIPEITHHPLNTTVIRGEPLTLECRSSGFPPPQIRWFKNGSPLFTSPDR-----MILPDGSLLFLRVFHSKKETDSGDYHCTATNSQGS--ASSHIATLEIAYMKDE----FKALPRDTEATLGRDIVLECSPPAGHPEPVVKWKRDGDHLDLTSSKRIKIDGQGNLLIQSVQKSDEGRYQCSAGNIANKKMSKA 203
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000001666 (supercontig:LSalAtl2s:LSalAtl2s128:488193:500323:-1 gene:EMLSAG00000001666 transcript:EMLSAT00000001666 description:"maker-LSalAtl2s128-augustus-gene-5.5") HSP 1 Score: 167.933 bits (424), Expect = 1.696e-42 Identity = 230/958 (24.01%), Postives = 358/958 (37.37%), Query Frame = 0
Query: 35 NEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHK--------SHRVLLPS----GSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAI-FECGPPKGSPTPTSFWKKDGRILELELDPS--------ICWVQKIQGNSPSEE---QKEKEKLFEARK---DEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLE----AIMGNFGRQLIRLGKR----PEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPA-----QRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-PPVFLVKPTD-QKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQTRVE----WLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHW-VAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPN-TEYSVFMLPYN----GRIKGRPTKLVMVTTKEDVPTAAPENVK-IQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEALNFTLEP----SSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVEL 935
++P + CEA+G+P P +W K+GEP D + + + P G +F R V+ G Y C ATN +G S N+ V + F EP ++ G C P G P P+ +W K + L+ S W I SE+ F +++ + + L + + + A R + +G E I G IR K+ P R L G+ TL+I V +D G Y C A N GI S + + P + PE + G++V F C G P P I+W + R T D ++T+ N+ D Y C A N G+V ++ V PP V P + + V G + + C G P PI+ W L G G V G L I + D+G YTC + ++ G AS + ++ I GP + G S F C A + +E WLK+G I +PR D +L + DS YTC AKS+ A SA L + + P+ PS + +N S V+ W P+ +PI +Y++ Y TS TP W VA V +S+ P T V ARN G S PS S+ TP + +P + E + + + W + PQ Q R D D+ +I + D +P + Y + ++ +N ++K +P V+ + E+ P+ AP N + ++ +A + W ++GD KG+K+ N + F P ++ + + L YA +V A+N GP + +E+
Sbjct: 51 DKPFIIECEAEGEPAPMYRWEKNGEPYDWQVYDERISQQPGRGTFLITKPRDEEIGEIFDYRRVE-------GQYQCFATNEWGTATS-NSVYVRKSELNNFVDEPPKTKTVEEGEPFGIPCNSPTGWPKPSVYWLKMSQNWHKTLNSSRITVDPEGTLWFSNITKEDSSEDFLYACSASSYFRNEYKVGNKVHLQVIQSGSNAGLQNKREPDFQYANKRSEIAL-RGKEMKLWCIFGGTPLPEIRWRKQGGPLPPGRTTLDNYGK-TLVIKHVDFEDAGDYTCEASNGVGIAKSYSISIEVHARPRFIVEPEIMITTEGESVDFLCQADGYPTPSIQWIYNGAKIEKAPPHPNRFITPD--RITITNVSKSDTGNYGCNASNVNGYVYKDVYVNVLDLPPEITVPPEETAQTVDGKAMTMKCETFGAPKPIVKWFHNNEE---LTG---GRYKVQDSGDLLIQDVKFFDAGNYTCKAENKFGKASASGTLTVKEHTR-----ISTGPKDYEVEAGDSATFRCNAVYDSELELDIHWLKNGKLIDFDIEPRFIQSSDQSLTITKTTELDSGQYTCLAKSSLDSAQASASL---------------IVQDVPNPPSLLW-VNCNSEDAVVTW-APMGDNRAPILNYKML------------------------------YNTSFTPDTWEVATDTVPASDTSFKVSMSPWTNYTFKVIARNQVGDSFPSGHSETCLTPERI--PFKNPDNV-------------EGRGTSPNNLVIYWTPM-PQIEHNAPKFQYRVYWKKDEEDASWIIEDIADWRVKELVIDNQPTYSRYQIKVVAHNRIGEAKMKAKP---VIGWSGENQPSEAPLNFTLLDVVGPRSAYVSWDPVSLDSINGDFKGYKIQTWTNSSGEEKFREIPWGKDTTEAFVQSFKPFSLNYA-RVLAFNGAYNGPPSNIIEV 918 HSP 2 Score: 72.7886 bits (177), Expect = 3.964e-13 Identity = 92/393 (23.41%), Postives = 148/393 (37.66%), Query Frame = 0
Query: 34 RNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE-------LDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQI--------------ELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGC--AVKGRPHPEIRWSRRNGMLPAQ---RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHL---QVFSPPVFL 397
R + + L C G P P+I+W K G P+ ++ G ++ V + D G Y CEA+N G +S + S+ V + +F +EP+ + +G + F C G PTP+ W +G +E + P + + K + + Y LD P+I + + C E F PK I+ F G R ++ G L+I V+ D G Y C A+N G + SA L+++ + P++ AGD+ F C +I W + ++ R + LT+ D YTCLA++ +ASA L V +PP L
Sbjct: 268 RGKEMKLWCIFGGTPLPEIRWRKQGGPLPPGRTTLDNY------GKTLVIKHVDFE---DAGDYTCEASNGVGIAKSYSISIEVHA-RPRFIVEPEIMITTEGESVDFLCQ-ADGYPTPSIQWIYNGAKIEKAPPHPNRFITPDRITITNV--------SKSDTGNYGCNASNVNGYVYKDVYVNVLDLPPEITVPPEETAQTVDGKAMTMKC--ETFGAPK---PIVKWFHNNEELTGGR------YKVQDSGDLLIQDVKFFDAGNYTCKAENKFG-KASASGTLTVKEHTRISTGPKDYEVEAGDSATFRCNAVYDSELELDIHWLKNGKLIDFDIEPRFIQSSDQSLTITKTTELDSGQYTCLAKSSLDSAQASASLIVQDVPNPPSLL 629 HSP 3 Score: 60.4622 bits (145), Expect = 2.519e-9 Identity = 45/154 (29.22%), Postives = 73/154 (47.40%), Query Frame = 0
Query: 20 PPVITEHPQNSSVTRN-EPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIE--PQDVGAIQGGTAIFECGPPKGSPTPTSF-WKKDGRILELELDP 169
PP IT P+ ++ T + + +T++CE G P+P +KWF + E + + LL FF D G Y C+A N FG+ S + +L V K+ RI P+D G +A F C S W K+G++++ +++P
Sbjct: 438 PPEITVPPEETAQTVDGKAMTMKCETFGAPKPIVKWFHNNEELTGGRYKVQDSGDLLIQDVKFF----------DAGNYTCKAENKFGKA-SASGTLTV---KEHTRISTGPKDYEVEAGDSATFRCNAVYDSELELDIHWLKNGKLIDFDIEP 577
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000005601 (supercontig:LSalAtl2s:LSalAtl2s2:790586:820681:1 gene:EMLSAG00000005601 transcript:EMLSAT00000005601 description:"snap_masked-LSalAtl2s2-processed-gene-8.21") HSP 1 Score: 140.584 bits (353), Expect = 5.776e-34 Identity = 221/979 (22.57%), Postives = 361/979 (36.87%), Query Frame = 0
Query: 22 VITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQ-DVGAIQGGTAIFECGPPKGSPTPTSFWK-----------------KDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDT----RPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAK--NVAGIRLSAKARLSLQVLPYVLKHPE-NVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTT------DGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQV-FSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT-SQDRPPPI---IQIGPVNQTQ-RIGSSTNFPCEATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQ--LVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTP--PKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQK---FVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLI------DINGTEALNFTLEP------SSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHP 944
+I+ S V L+C+A G P+PQ +W +DG V+L S +QS + D G Y C A NT G + S + VA + F P D+ G AIF+ P P+ W+ D IL ++ S + + +E+ E ++ + PK T P EL N + L I + + + + + + + + TL + +V G Y C + + G L+ A + + PY + G+ + C VKG P P + W RNG +P V T + L + + ED ++ C A N AG V L+V S PV + P + + G V IPC A G P+P + W G G + + +G + I D G Y+C + +G + + + Q PP+ + +G V ++ S + P E V W +G I+ RI + D TL E SD+ YTC+ KST G A L V E P +P+ Y + Q + + W P G SPI + ++ +S P+ W + ++SS T+RT + + ++SS + + A N G P S S P P +G + + S S+ +M + W+ Q + GY I+ R DS SY +S R++ +T+L +Y + + +N + G + + + T+E VP +AP V+ Q ++ + + W P + ++G +G+K+ D + T+ P S + ++ NL Y V V Y G G P + D P
Sbjct: 41 IISPSASGSIVAERRTKILQCQAVGSPQPQYRWTRDG--------------VVLSEFSSETFYKIQSVMKADAGNYQCAARNTAGTILSDTIPITVAYMT-PFDPAPNPDLQVKAGHAAIFKFPVLASVPEPSVIWQTDDGTQLYGVKYAMTSENDLVILSVDSSDSRRYRTRATNTQLGQEENSPYGFLEVYGEDGADVPPEIVIPPKDLTVNKDTPVSELQCIAN------ARPLSEIELTWHKDGVPIDET-QVSYSFNDLWNRTLSLLRVNLDHSGVYSCQVRMRSGGGPTLTKDATVKVTEKPYFYRRMSLETFGDFGNDISIPCHVKGIPEPSVIW-YRNG-IPIDEVPNTRYSVDPEDRSLKINFMRNEDSGMFQCSAVNYAGDVNGYTWLRVKTSSPVLVDPPKNTSALDGKDVSIPCKAEGAPLPNVTWYFNDGQITY-----SGRIQILEDGSMLISKVRSTDQGKYSCIRSNTAGNTRGEAWLGVLVRXQITSPPVDSKVILGHVASLHCKVSSDQDVPYE------VHWYHEGRMINAAMSHRINILDDGTLKIAEARASDAGDYTCEVKSTGGNDHRVAALDVI---------------ELPYAPTRIYAERVANAQKSVNVSW-TPGFDGNSPIIKFIIQYHSVPQSGPIPKDDLNWITALANISS------TART----ILLSNLKSSAAYIF-----------RLSAVNSVGEGPQSLPSNRVVLPQEPPSGPPLG---LVGSARSETEIM--------------IQWQPPNEQAQNGDILGYIIRYRLFGYNDSPWSYR--NVSKPLQRNYLITELITWKDYEIQIAAFNNKGTGAYSSSIKLKTREGVPYSAPSGVRAQAIDSSVVRVWWLPPDPQKINGINQGYKLQAWIGDPRDTDIKPQSTVTVAPDLLNPLSEQTAVIDNLKPWTAYNVTVLCYTSPGDGKRSPPETVRTYQDYPGP 928 HSP 2 Score: 83.1889 bits (204), Expect = 2.773e-16 Identity = 107/491 (21.79%), Postives = 193/491 (39.31%), Query Frame = 0
Query: 123 KFRIEPQDVGAI--QGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKI-QGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDF-------DCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIR--------LSAKARLSLQVLPYVLKHPENVSAVAGDT--VRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTD------GTQLTLMNIVPEDEDVYTCLAENRAG---FVEASAHLQVFSPPVFLVKPT-DQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGM-LRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
+F I P G+I + T I +C GSP P W +DG +L + +Q + + ++ + + + D I +T++Y D P +L V P+ + G QL + ++ E L+I V D +Y+ A N + L V P ++ P++++ V DT C RP EI + +P + L+L+ + + VY+C R+G + A ++V P F + + + G G+ + IPC G P P + W + G +P + V PE L+I +DSG++ C++V+ +G + + ++++TS P++ P N + G + PC E P V W + I+ RI++ D ++ ++ +D Y+C +T+G A+L V
Sbjct: 39 RFIISPSASGSIVAERRTKILQCQA-VGSPQPQYRWTRDGVVLSEFSSETFYKIQSVMKADAGNYQCAARNTAGTILSDTI---PITVAYMTPFDPAPNPDLQVKAGHAAIFKFPVLASVPEPSVIWQTDDGTQLYGVK--------YAMTSENDLVILSVDSSDSRRYRTRATNTQLGQEENSPYGFLEVYGEDGADVPPEIVIPPKDLT-VNKDTPVSELQCIANARPLSEIELTWHKDGVPIDETQVSYSFNDLWNRTLSLLRVNLDHSGVYSCQVRMRSGGGPTLTKDATVKVTEKPYFYRRMSLETFGDFGNDISIPCHVKGIPEPSVIWYRNGIPIDEVPNT---RYSVDPEDRSLKINFMRNEDSGMFQCSAVNYAGDVNGYTWLRVKTSS----PVLVDPPKNTSALDGKDVSIPCKAEGAPLPNVTWYFNDGQITYSG--RIQILEDGSMLISKVRSTDQGKYSCIRSNTAGNTRGEAWLGV 507 HSP 3 Score: 68.5514 bits (166), Expect = 8.664e-12 Identity = 68/239 (28.45%), Postives = 110/239 (46.03%), Query Frame = 0
Query: 705 ARNLHGLSPPSPISKPFQT---PPK-AGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLS---WELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEALNFTLE----PSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDP 932
A N+ G S PS ++ FQT PPK A ++++ R M E+++ R + LS W IP+ + Y I +D S+L+S I+I + L L T Y V + YN P+ +++ T+E VP A P +V + + T +++W + +R +G L+G+KV G + + F + ++ L L + YA+QV AY R G G L P
Sbjct: 1100 ANNVVGASEPSEPTRQFQTIQAPPKHAPQNVT-----------IRSMXATELRV---RWIPLSQGEW-YGIPRGYNISYRIN-KDSSELNS------ISIEDPTRNTFVLDGLEEFTLYEVVLHAYNDLGTSDPSTVILARTREAVPGAGPNDVSAEATSSTTILVRWGDVLKRHSNGILEGYKVYF---GAKGVPFQYKNIGSNQTHQTTLTELKKYTEYAIQVLAYTRIGDGALSAP 1313
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000006220 (supercontig:LSalAtl2s:LSalAtl2s33:28635:33298:1 gene:EMLSAG00000006220 transcript:EMLSAT00000006220 description:"maker-LSalAtl2s33-augustus-gene-0.24") HSP 1 Score: 127.872 bits (320), Expect = 4.474e-30 Identity = 116/396 (29.29%), Postives = 169/396 (42.68%), Query Frame = 0
Query: 127 EPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCP-KLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVL-----PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPA-QRVHTTD-GTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPP---VFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVH-VSPEGMLRIGNTSEKDSGVYTC-----ASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQ 505
EP D GG+ F C G P+P WKKDG+I+ S + G S ++ A ++G SY P + D + + C F P G G+ S+ +GTL I+ Q D Y+C A N G R S+ AR+ + + P +P N ++ G V F C G P+P+ W + N ++ + R+ +D G L + N+ +D YTC AEN G E SA+L V S P V L P D GSTVQ+ C A+G P P + W K+G P + + +SP+G L + N S +G Y C A +S A+L+ V I + P P ++ V QT+
Sbjct: 19 EPTDADVNPGGSTFFTC-KASGVPSPFIIWKKDGQIV------SRSRTSLVNGES-------TLRISSASLSDVG------SYHPTRTDVLKGETIRLTCGATGFPTPTFSWFKDG---------GRLSSFHDRFSVGEDGTLTITNAQLDDSAXYRCSATNYLG-RASSAARVRVNLAIPDSKPIFTSNPRNAKSMEGGIVEFTCISTGHPYPDTTWWKNNRIVSSDNRITVSDSGQHLRIQNVRLQDRGEYTCRAENAHGISEVSANLTVQSRPTPIVMLEIPHDMNAGAGSTVQLACRASGYPKPSITWKKDG-----RPITESYLAYRLSPDGSLILFNVSRVYNGNYQCVASNDAETKQSNAVLS---VFIDKEKLYPLPSSRLSSVPQTE 376 HSP 2 Score: 108.612 bits (270), Expect = 4.119e-24 Identity = 88/332 (26.51%), Postives = 138/332 (41.57%), Query Frame = 0
Query: 262 IEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPA--QRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVF-----SPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGM-LRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIG-PVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTC----QAKSTSGQAMLSAFL 578
+ GE TL IS D G Y HP + G+T+R C G P P W + G L + R + LT+ N +D Y C A N G ++A ++V S P+F P + K ++G V+ C++ G+P P W K + ++ N+ + VS G LRI N +D G YTC + + G S+ + +Q+ RP PI+ + P + GS+ C A+ P+ + W KDG PI T + ++ D +L + + Y C A++ A+LS F+
Sbjct: 62 VNGESTLRISSASLSDVGSY----------------------------HPTRTDVLKGETIRLTCGATGFPTPTFSWFKDGGRLSSFHDRFSVGEDGTLTITNAQLDDSAXYRCSATNYLGRASSAARVRVNLAIPDSKPIFTSNPRNAKSMEGGIVEFTCISTGHPYPDTTWWK---NNRIVSSDNR--ITVSDSGQHLRIQNVRLQDRGEYTCRAENAHGISEVSANLTVQS---RPTPIVMLEIPHDMNAGAGSTVQLACRASGYPKPSITWKKDGRPI-TESYLAYRLSPDGSLILFNVSRVYNGNYQCVASNDAETKQSNAVLSVFI 356 HSP 3 Score: 86.2705 bits (212), Expect = 3.017e-17 Identity = 70/282 (24.82%), Postives = 112/282 (39.72%), Query Frame = 0
Query: 305 PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLV---KPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKV-DHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
P + K P + G + F C G P P I W +++G + ++ +R V + L++ S + V PT ++G T+++ C A G P P W K+GG S V +G L I N DS Y C++ + G +++ V++ + PI P N G F C +T P W K+ +S+ D RI V D L + + + D YTC+A++ G + +SA L V
Sbjct: 14 PLITKEPTDADVNPGGSTFFTCKASGVPSPFIIW-KKDGQI----------------------------VSRSRTSLVNGESTLRISSASLSDVGSYHPTRTDVLKGETIRLTCGATGFPTPTFSWFKDGGR----LSSFHDRFSVGEDGTLTITNAQLDDSAXYRCSATNYLGRASSAARVRVNLAIPDSKPIFTSNPRNAKSMEGGIVEFTCISTGHPYPDTTWWKNNRIVSS--DNRITVSDSGQHLRIQNVRLQDRGEYTCRAENAHGISEVSANLTV 260 HSP 4 Score: 63.929 bits (154), Expect = 2.152e-10 Identity = 71/296 (23.99%), Postives = 113/296 (38.18%), Query Frame = 0
Query: 17 AASPPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSR---NASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEI-GTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRP--------------EARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLS 294
A+ V + HP + V + E + L C A G P P WFKDG + ++ H V G+L + + + +D Y C ATN GR S +L + K F P++ +++GG F C G P P + W K+ RI + D I Q + + + R + G ++ + + P + L + P + A G+ + R P E+ + +G+LI+ V G YQCVA N A + S
Sbjct: 73 ASLSDVGSYHPTRTDVLKGETIRLTCGATGFPTPTFSWFKDGGRL---SSFHDRFSV-GEDGTL----TITNAQLDDSAXYRCSATNYLGRASSAARVRVNLAIPDSKPIFTSNPRNAKSMEGGIVEFTC-ISTGHPYPDTTWWKNNRI--VSSDNRITVSDSGQHLRIQNVRLQDRGEYTCRAENAHGISEVSANLTVQSRPTPIVMLEI---------PHDMNAGAGSTVQLACRASGYPKPSITWKKDGRPITESYLAYRLSPDGSLILFNVSRVYNGNYQCVASNDAETKQS 348
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000005454 (supercontig:LSalAtl2s:LSalAtl2s293:185286:259880:1 gene:EMLSAG00000005454 transcript:EMLSAT00000005454 description:"maker-LSalAtl2s293-augustus-gene-2.25") HSP 1 Score: 126.331 bits (316), Expect = 2.131e-29 Identity = 168/719 (23.37%), Postives = 291/719 (40.47%), Query Frame = 0
Query: 268 LIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYV--LKHPENVSAVAGDTVRFGCAVKGRPHP-EIRWSRRNGML--PAQRVH---TTDGTQLTLM---NIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQ--------GHVHVSP-------EGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKST--SGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKF---VTGYHIQLRDISDLDSSDS-YDVITISGGGTRSHTL--TDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEALNFTLEPSSNSLI--------LYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHP 944
L+I+ V + G Y C A N AG S A+L + P + L +NV + G + C + P +I WS L Q + T G++ +++ ++ + YTC A N+AG +A L++ P + V+PTD+ QGS +I C + G P P + W K G LP SN+ + VS +G L I N + + G Y C + + G I A +++ +Q PP +I QT I +T CEA + + L + MN+ R+ D+ E I D L T K T A+ + A ++ + + + E P +P + +++ + + W P G S + Y + T W + H V +P Q+ +Y +PA + H + A+N+ G+S PS T A E S P D RV + + + +++SW+ + + + + GY++ + ++ + ++ + H L ++L TEY+V + +N +G + + E PT +P+++ + L+ + + W PP HG +KG+KV I G + + +PSS++ L L Y++QV A+ G G + E D+ P
Sbjct: 1968 LVINSVGYRHRGNYTCXASNNAG-HDSYTAKLRVNEPPSLVPLNFGKNVFS-EGSFAQVVCTITEGDEPIKISWSFHGESLEDSGQNIGIAITNIGSRTSILIINSVEHKHRGNYTCSASNKAGNSSDTAQLKINVRPRWTVEPTDKHFAQGSEAKIDCKSDGFPQPKIVWRKAIG----LPVSNEQXPTPSDYKDLDVSNDRNMKIIDGTLIIKNIQKSNEGFYLCKASNGIGGISAVAKISVQA-----PPSFEIKKRTQTALISDNTVLQCEAKGEKPIGVLWN------MNNKRLDAKVDDRYTIREEVIDDGVLSTISIKRTERGDSALFTCVATNAFGSDDTSINLI--IQENPETPYDLKVLDKAGRTVKLSWQTPYN-GNSNLTRYVIE---------FKPTKGSWKNDIDH-----------------VLVPGDQNQA--VVYSLKPANSYHFRIVAQNVIGVSGPSDT----ITIETAEEAPSGPPV------DIRVSAVDQ------QTLRVSWKPPLTEHWNGDILGYYVGYKATANSEEKPYLFETVEFRKEQGHEHQLQISNLEVFTEYAVVVQAFNKIGQGPMSXEXLXHXAEGAPTESPQDIDLVTLSSQSIKVTWSSPPLASAHGIIKGYKV---IYGPSKVWY--DPSSHATKISSDMQAELTGLQRYTNYSIQVLAFTNGGDGTKTEVFTTTTEQDIPGP 2617 HSP 2 Score: 89.7373 bits (221), Expect = 2.905e-18 Identity = 145/608 (23.85%), Postives = 246/608 (40.46%), Query Frame = 0
Query: 19 SPPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEP-------QDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQ--KEKEKLFEARKDEIGTESLTLSYCPKLDT---------RPQIELVVACNREDFDCPKGLEAIMG----NFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQ-YQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAG-DTVRFG------CAVKGRPHP-EIRWSRRNGMLPAQRVHTTD-----GTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGV--QGSTVQIPCVAAGNPVPI-LFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA----TPQTRVEWLKDGVPIST---MNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
+P + P+ V VT +C +G+P ++ W K+G+ + +H + LR + S K++D G+Y C N + ++ A LK R +P ++ GG C KG P+P W G+ ++ E ++ + G+ S + K + + + ++ +S+ KL+ RP VVA CP I GRQL ++ ++ GTLIIS +Q K++ Y CVA N G AR L+V V++ PE V G D+V G C V+ P +I WS + + ++ TT + L++ ++ YTC A+N+AG + S L V PP L+ T + + +G Q+ C+ + P+ + W +G PG + + +L I + + SGVYTC S +E+G S+E+K+ + PP ++ + + G C P T + W G IS+ ++ ++ V + + S YTC + +G SA L+V
Sbjct: 184 APLSVDVEPKTQVVDYGRSVTFKCNYKGNPIKEVFWLKNGKSI-----NHNENT----------LR-INSVKKDDRGMYQCFIRN------EQESAQATAELKLGGRFDPPEFISHFEETIVKPGGYLSISC-IAKGDPSPNIKWFIYGKEVKNEGAITVGSFRDANGDVTSHLNISQVKTQFGGTYECQASSKVGKISHMAKLNVFGAPYVKKMRPM--KVVAGKSMFITCPVAGYPISNIVWEKDGRQLPFNDRQ-------TVFPNGTLIISDIQRKEDASVYTCVAXNDEGY----SARSDLEV--SVMERPEIVPFFFGSDSVDEGLYAQVSCIVQRGDTPIKISWSLKGDTVSSEPGMTTSQVGPRASILSIHSVGYRHSGTYTCTAQNKAGIISYSTSLTVNEPPS-LMPLTFGRDILDEGEFAQLICIVSKGDEPLTMSWTLKGDDVSSEPGLSTTKIGTRTS-ILTINSVGYRHSGVYTCTSQNEAGVATQSAELKV----NEPPQLMPLTFGDDVLNEGEFAQLFCIVRVGDEPLT-LTWALQGDVISSEPGLSTTQLAVGYXH-----------SGTYTCTVTNKAGSDSQSAILKV 735 HSP 3 Score: 84.3445 bits (207), Expect = 1.375e-16 Identity = 101/397 (25.44%), Postives = 169/397 (42.57%), Query Frame = 0
Query: 242 NFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAG-----IRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQV---FSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEG----GSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELE-ISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRI 625
N ++L++L R + GTLII + + +D G+Y C+ N G L+ A LS+ V P+ G +V F C KG P E+ W +NG + ++ + T L + ++ +D +Y C N +A+A L++ F PP F+ + G + I C+A G+P P + W G G + GS + + L I + G Y C + S+ G I +++ + + P + ++ P+ P P + + W KDG + ND R V + TL +++ D+++YTC A + G + S L V+ E + VPF F S S + A + +V D PI+I
Sbjct: 131 NNNKKLVKLDDRVKXV-------GGTLIIKEAKVEDSGKYLCMVNNSVGGESVETVLTVTAPLSVDV------EPKTQVVDYGRSVTFKCNYKGNPIKEVFW-LKNG----KSINHNENT-LRINSVKKDDRGMYQCFIRNEQESAQATAELKLGGRFDPPEFISHFEETIVKPGGYLSISCIAKGDPSPNIKWFIYGKEVKNEGAITVGSFR-DANGDVTSHLNISQVKTQFGGTYECQASSKVGKISHMAKLNVFGA----PYVKKMRPMKVVAGKSMFITCPVAGYPISNIVWEKDGRQLP-FND-RQTVFPNGTLIISDIQRKEDASVYTCVAXNDEGYSARSD-LEVSVMERP---EIVPF---FFGSDSVDEGLYAQVSCIVQRGDTPIKI 494 HSP 4 Score: 82.4185 bits (202), Expect = 5.097e-16 Identity = 109/470 (23.19%), Postives = 184/470 (39.15%), Query Frame = 0
Query: 145 PPKGSPTPTSFWKK------DGRILELELDPSICWVQKIQGNSPSEEQKEKE--KLFEARKDEIGTES----LTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVL-----PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRW------SRRNGMLPAQRVHTTDG---TQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEK-DSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC---EATPQTRVEWLKDGVPISTMNDPRIKVDH----DNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
P SP P+ W K + ++++L+ V+ + G +E K ++ K + +G ES LT++ +D P+ ++V F C GN +++ L E TL I+ V+ D G YQC +N + SA+A L++ P + H E G + C KG P P I+W + G + +G + L + + + Y C A ++ G + A L VF P ++ K K V G ++ I C AG P+ + W K+G LP +++ V P G L I + K D+ VYTC + ++ G S+ ++ S P I+ + + G C ++ W G +S+ +P + + L + S YTC A++ +G S L V
Sbjct: 113 PAMASPIPSFRWYKFMEANNNKKLVKLDDR-----VKXVGGTLIIKEAKVEDSGKYLCMVNNSVGGESVETVLTVTAPLSVDVEPKTQVVDYGRSVTFKC-----NYKGNPIKEVFWLKNGKSIN-----HNENTLRINSVKKDDRGMYQCFIRNE---QESAQATAELKLGGRFDPPEFISHFEETIVKPGGYLSISCIAKGDPSPNIKWFIYGKEVKNEGAITVGSFRDANGDVTSHLNISQVKTQFGGTYECQASSKVGKISHMAKLNVFGAP-YVKKMRPMKVVAGKSMFITCPVAGYPISNIVWEKDGRQ---LPFNDRQTVF--PNGTLIISDIQRKEDASVYTCVAXNDEG---YSARSDLEVSVMERPEIVPFFFGSDSVDEGLYAQVSCIVQRGDTPIKISWSLKGDTVSS--EPGMTTSQVGPRASILSIHSVGYRHSGTYTCTAQNKAGIISYSTSLTV 553 HSP 5 Score: 76.6406 bits (187), Expect = 3.179e-14 Identity = 87/329 (26.44%), Postives = 130/329 (39.51%), Query Frame = 0
Query: 268 LIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVA-GDTVRFGCAVKGRPHP-EIRWSRRNGMLPAQRVHTTD--GTQLTLM---NIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGV-QGSTVQIPC-VAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC---EATPQTRVEWLKDGVPISTMNDPRIKVDHDNT----LHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
L+I+ V + G Y C A AG + A L + P +L + V GD + C V P I WS + ++ ++ TT GT+ +L+ N+ YTC A N AG SA L V P L V +G Q+ C V+ G+ + W +G P + ML I N + SG YTC + + G S+E+K+ + PP I + + + G C + R+ W G +S+ +P I T L + S YTC+A + +G A SA L V
Sbjct: 991 LMITNVGYRHSGTYTCRATXSAG-XATHSAELKVNESPSILPFSFGSNNVKEGDITQLTCLVSRGDEPVRITWSLKGDVISSEPSITTTMFGTRTSLLMITNVGYRHSGXYTCRASNAAGXATHSAELNVNESPSILAFSFGSDSVKEGDMTQLTCFVSRGDEPLTITWSLKGDVVSSEPSITTTMIGTRVS-MLMITNVGYRHSGTYTCRATNSGGTATHSAELKV----NEPPIINEFSFGSNSVNEGDMAQLACFVSKGDEPLRITWSLKGDVVSS--EPSITTTMIGTRTSMLMITNVGYRHSGSYTCRASNAAGSATHSASLLV 1311 HSP 6 Score: 72.0182 bits (175), Expect = 7.685e-13 Identity = 126/557 (22.62%), Postives = 202/557 (36.27%), Query Frame = 0
Query: 78 GSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVV----AVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQ------------LIRLGKRPEARFLLSIEGE-------------GT----LIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVA-GDTVRFGCAVKGRPHP-EIRWSRRNGMLPAQRVHTTD--GTQLTLM---NIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGV-QGSTVQIPCVAAGNPVPI-LFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQ-----IGPVNQTQRIGSSTNFPC---EATPQTRVEWLKDGVPISTMNDPRIKVDHDNT----LHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
GS + +QS GIY C+A N G +R ++ASL+V ++L F E G I T + +G T W G ++ PSI + R + ++ + R A + + + + +FG L+ G P R S++G+ GT L+I+ V + G Y C A N AG + A L++ P +L +V GD + C V P I WS + ++ ++ TT GT+++++ N+ YTC A N G SA L+V PP+ V +G Q+ C + P+ + W +G P + ML I N + SG YTC + + +G+ S+ + + + PPII PVN+ G C + W G IS+ +P + T L ++ S YTC+A + G SA L++
Sbjct: 889 GSRXSMLXIQSVGYRHNGIYTCQAKNEAGSMR-QSASLIVNESPSILPFSFGAESVKEGDINQLTCLVN----RGDEPLTITWSLKGDVVSSX--PSIT-----------------TTMIGTRASMLMITNVGYRHSGTYTCRATXSAGXATHSAELKVNESPSILPFSFGSNNVKEGDITQLTCLVSRGDEP-VRITWSLKGDVISSEPSITTTMFGTRTSLLMITNVGYRHSGXYTCRASNAAG-XATHSAELNVNESPSILAFSFGSDSVKEGDMTQLTCFVSRGDEPLTITWSLKGDVVSSEPSITTTMIGTRVSMLMITNVGYRHSGTYTCRATNSGGTATHSAELKVNEPPIINEFSFGSNSVNEGDMAQLACFVSKGDEPLRITWSLKGDVVSSEPSITTTMIGTRTS-MLMITNVGYRHSGSYTCRASNAAGSATHSASLLV-----KEPPIINPFSFGAEPVNE----GDVAQLTCLVRRGDEPLSITWSLKGDIISS--EPSMTTTMFGTKASMLMITDVGYRHSGTYTCRASNDGGFDTYSAELKI 1407 HSP 7 Score: 71.633 bits (174), Expect = 9.818e-13 Identity = 87/331 (26.28%), Postives = 136/331 (41.09%), Query Frame = 0
Query: 268 LIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAV-AGDTVRFGCAV-KGRPHPEIRWSRRNGMLPAQRVHTTD--GTQLTLM---NIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPV-----FLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRI---GSSTNFPCEAT----PQTRVEWLKDGVPISTMNDPRIKVDHDNT----LHFEELEISDSALYTCQAKSTSGQAMLS 575
L+I+ V + G Y C A N G + A L + P + + ++V GD + C V KG I WS + ++ ++ TT GT+ +++ N+ YTC A N AG SA L V PP+ F +P + +G Q+ C+ P+ G + S + + ML I + + SG YTC + ++ G S+E+KI + PP QI P + + + GS C T P T + W G IS+ +P I T L E+ S +TC+A + +G S
Sbjct: 1183 LMITNVGYRHSGTYTCRATNSGGTA-THSAELKVNEPPIINEFSFGSNSVNEGDMAQLACFVSKGDEPLRITWSLKGDVVSSEPSITTTMIGTRTSMLMITNVGYRHSGSYTCRASNAAGSATHSASLLVKEPPIINPFSFGAEPVN----EGDVAQLTCLVRRGDEPLSITWSLKGDIISSEPSMTTTMFGTKASMLMITDVGYRHSGTYTCRASNDGGFDTYSAELKI----NEPP---QIAPFSFSPHVIDEGSYAQISCSVTKGDEPLT-ITWSLHGAVISS--EPSITTTMIGTRTSILIISEVGYRHSGKFTCRASNPAGSVRYS 1498 HSP 8 Score: 69.707 bits (169), Expect = 4.378e-12 Identity = 85/332 (25.60%), Postives = 129/332 (38.86%), Query Frame = 0
Query: 268 LIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSL----QVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHP-EIRWSRRNGMLPAQRVHTTD-----GTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGV-QGSTVQIPCVAAGNPVPI-LFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC---EATPQTRVEWLKDGVPISTMNDPRIKVDHDNT----LHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
L I + + G Y C A N AG S A L + ++LP P S G+ + C V P I WS ++ ++ +T+ + L + ++ +YTC A+N AG + SA L V P L + V +G Q+ C+ P+ + W +G P + ML I N + SG YTC + +G S+E+K+ S P I+ + + G T C R+ W G IS+ +P I T L + S YTC+A + +G A SA L V
Sbjct: 799 LTIQSIGYRHSGVYTCKATNKAG-SGSQXAVLRVNEPPEILPLSFGKP---SMDEGEFAQIVCIVSRGDEPLSITWSLYGAVISSEPSISTNLLGSRXSMLXIQSVGYRHNGIYTCQAKNEAGSMRQSASLIVNESPSILPFSFGAESVKEGDINQLTCLVNRGDEPLTITWSLKGDVVSSXPSITTTMIGTRAS-MLMITNVGYRHSGTYTCRATXSAGXATHSAELKVNES----PSILPFSFGSNNVKEGDITQLTCLVSRGDEPVRITWSLKGDVISS--EPSITTTMFGTRTSLLMITNVGYRHSGXYTCRASNAAGXATHSAELNV 1119 HSP 9 Score: 63.929 bits (154), Expect = 2.356e-10 Identity = 70/272 (25.74%), Postives = 101/272 (37.13%), Query Frame = 0
Query: 326 CAVKGRPHPEIRW-----SRRNGMLPA--QRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKV--------DHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
C P P RW + N L RV GT L + ED Y C+ N G L V +P V+P Q G +V C GNP+ +FW+K G S N E LRI + + D G+Y C +E + A++E+K+ D P I + G + C + P ++W G + N+ I V D + L+ +++ Y CQA S G+ A L V
Sbjct: 112 CPAMASPIPSFRWYKFMEANNNKKLVKLDDRVKXVGGT-LIIKEAKVEDSGKYLCMVNNSVGGESVETVLTVTAPLSVDVEPKTQVVDYGRSVTFKCNYKGNPIKEVFWLKNGKS------INHN------ENTLRINSVKKDDRGMYQCFIRNEQESAQATAELKLGGRFDPPEFISHF--EETIVKPGGYLSISCIAKGDPSPNIKWFIYGKEVK--NEGAITVGSFRDANGDVTSHLNISQVKTQFGGTYECQASSKVGKISHMAKLNV 366 HSP 10 Score: 62.3882 bits (150), Expect = 6.381e-10 Identity = 85/354 (24.01%), Postives = 138/354 (38.98%), Query Frame = 0
Query: 268 LIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYV--LKHPENVSAVAGDTVRFGCAVKGRPHP-EIRWSRRNGMLPAQRVHTTDG----------TQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKP---TDQKGVQGSTVQIPCVAAGNPVPI-LFWMKEGGSGVLLPGSNQGHVHVSPE-GMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTS-------------QDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQT---RVEWLKDGVPISTMN-------DPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
LIIS V + G Y C AKN+AG+ ++ + L + P + +N+ GD + C V P I WS + + T G + L + + + YTC A N+AG SA +V PP L+ P ++ +G Q+ CV +P+ + W G + + P + ++ P +L I + G Y C + +++G S+E+K+ + PP I N+ G C T + W G I T PR+ V N++++ S YTCQAK+ +G +A L+V
Sbjct: 1567 LIISSVGYRHSGDYTCNAKNIAGM-VTYTSTLLVNEAPEIGPFSFSKNIMN-EGDFAQLTCVVNRGDLPLTITWS-----FHGENIGTNTGIITSNLGPRMSMLVINSAQHHHQGEYTCQAINKAGIRTHSAEXKVNEPP--LIAPFSFANEVLNEGDFAQVTCVVNRGDLPLTITWSFHGEN--IGPNTGISTNNLGPRISLLVINSAQHHHQGEYICQAQNKAGIQTHSAELKVNGTFILMRVADYFYFWFSEPPLIAPFSFANEVMNEGDFAQISCVITSGDLPLLITWSFHGNTIGTNTGIATTNLGPRMSVLAINSVNY-----SHQGKYTCQAKNKAGIRTHTAELKV 1904 HSP 11 Score: 61.6178 bits (148), Expect = 1.007e-9 Identity = 76/325 (23.38%), Postives = 134/325 (41.23%), Query Frame = 0
Query: 268 LIISQVQPKDEGQYQCVAKNVAGIRL-SAKARLSL--QVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHP-EIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGV-QGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC---EATPQTRVEWLKDGVPISTMNDPRIKVD----HDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
L I+ V + G Y C ++N AG+ SA+ +++ Q++P L ++V G+ + C V+ P + W+ L + + G T + + YTC N+AG SA L+V PP L Q+ + +G Q+ C+ P+ G + S + ML I + + SGVYTC + +++G+ + +++ + PP I+ + + G C + W G IS+ +P I + + L + + + +YTCQAK+ +G SA L V
Sbjct: 617 LTINSVGYRHSGVYTCTSQNEAGVATQSAELKVNEPPQLMP--LTFGDDV-LNEGEFAQLFCIVRVGDEPLTLTWA-----LQGDVISSEPGLSTTQLAVGYXHSGTYTCTVTNKAGSDSQSAILKVNEPPRILPLTFGQEVMNEGQFAQVVCIVTEGDEPLSIGWXLQGXXIXSXPSLSXSSLGTRTSMLTIQSIGYRHSGVYTCKATNKAGSGSQXAVLRV----NEPPEILPLSFGKPSMDEGEFAQIVCIVSRGDEPLSITWSLYGAVISS--EPSISTNLLGSRXSMLXIQSVGYRHNGIYTCQAKNEAGSMRQSASLIV 927 HSP 12 Score: 55.4546 bits (132), Expect = 7.837e-8 Identity = 79/360 (21.94%), Postives = 154/360 (42.78%), Query Frame = 0
Query: 268 LIISQVQPKDEGQYQCVAKNVAGIRL-SAKARLS-----LQVLPYV---LKHPENVSAVA--------GDTVRFGCAVKGRPHPE-IRWSRRNGMLPAQR-VHTTD-GTQLTLMNIVPED---EDVYTCLAENRAGFVEASAHLQVFSPPVF--LVKPTDQKGVQGSTVQIPCVAAGNPVPI-LFWMKEG---GSGVLLPGSNQGHVHVSPE-GMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFP------CEATPQTRVEW------LKD-----GVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
L+I+ Q +G+Y C A+N AGI+ SA+ +++ ++V Y P ++ + GD + C + P I WS + + TT+ G +++++ I + + YTC A+N+AG +A L+V PP+ P + +G+ Q+ CV +P+ + W G G+ + +N G P +L I + + G YTC + + +G ++++++ + PP ++ P+N + + S +F E ++ W L+D G+ I+ + + L +E YTC A + +G + +A L++
Sbjct: 1759 LVINSAQHHHQGEYICQAQNKAGIQTHSAELKVNGTFILMRVADYFYFWFSEPPLIAPFSFANEVMNEGDFAQISCVITSGDLPLLITWSFHGNTIGTNTGIATTNLGPRMSVLAINSVNYSHQGKYTCQAKNKAGIRTHTAELKVNEPPMIGPFAFPKEVMN-EGNFAQLTCVVTSGDLPLSVTWTFHGDKVGTQTGITTTNLG-----PRLSLLVINSVGYRHRGNYTCXASNNAGHDSYTAKLRV----NEPPSLV---PLNFGKNVFSEGSFAQVVCTITEGDEPIKISWSFHGESLEDSGQNIGIAITNIG------SRTSILIINSVEHKHRGNYTCSASNKAGNSSDTAQLKI 2099 HSP 13 Score: 55.4546 bits (132), Expect = 8.384e-8 Identity = 59/236 (25.00%), Postives = 98/236 (41.53%), Query Frame = 0
Query: 353 TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGV-QGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC---EATPQTRVEWLKDGVPISTMNDPRIKVDHDNT----LHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
T + LT+ +I VYTC A N+AG A L+V PP L + + +G QI C+ + P+ G+ + S ++ S ML I + + +G+YTC + +E+G++ S+ + + S P I+ ++ + G C + W G +S+ P I T L + S YTC+A ++G A SA L+V
Sbjct: 794 TRTSMLTIQSIGYRHSGVYTCKATNKAGSGSQXAVLRVNEPPEILPLSFGKPSMDEGEFAQIVCIVSRGDEPLSITWSLYGAVISSEPSISTNLLGSRXSMLXIQSVGYRHNGIYTCQAKNEAGSMRQSASLIVNES----PSILPFSFGAESVKEGDINQLTCLVNRGDEPLTITWSLKGDVVSSX--PSITTTMIGTRASMLMITNVGYRHSGTYTCRATXSAGXATHSAELKV 1023 HSP 14 Score: 53.1434 bits (126), Expect = 4.345e-7 Identity = 41/140 (29.29%), Postives = 62/140 (44.29%), Query Frame = 0
Query: 265 EGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQ-------RVHTTDGTQLTLMNIVPE---DEDVYTCLAENRAGFVEASAHLQVFSPP 394
+GTLII +Q +EG Y C A N G +SA A++S+Q P +A+ D C KG + W+ N L A+ R D L+ ++I D ++TC+A N G + S +L + P
Sbjct: 2170 DGTLIIKNIQKSNEGFYLCKASNGIG-GISAVAKISVQAPPSFEIKKRTQTALISDNTVLQCEAKGEKPIGVLWNMNNKRLDAKVDDRYTIREEVIDDGVLSTISIKRTERGDSALFTCVATNAFGSDDTSINLIIQENP 2308
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000003654 (supercontig:LSalAtl2s:LSalAtl2s199:341388:472828:1 gene:EMLSAG00000003654 transcript:EMLSAT00000003654 description:"maker-LSalAtl2s199-augustus-gene-4.26") HSP 1 Score: 123.25 bits (308), Expect = 1.210e-28 Identity = 179/780 (22.95%), Postives = 294/780 (37.69%), Query Frame = 0
Query: 233 PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEG------------EGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHP-EIRWSRRNGML-PAQRVHTTD---GTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVL-------LPGSNQGHVHVS-------PEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQT--RVEWLKDGV--PISTMNDPRIKVDHDN-------TLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSY--QVREGHC-WKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPI---------------SKPFQTPPKAGEDISDPR----YLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKV---LIDINGTEA----LNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDL 941
P+ A+ G L + P R + S EG GTL+I +V+ D Y CV +N G R K L P + D + C++ P +I W + +L P + + G+ L + + Y C+A N A ++ L V PP +L+ P D S V + C A+GNP P + W + S GS +V S P G L + + + +D G Y C + GAI S V +Q P G N+T G + C A + +EW K G+ I R + N TL + SDS + C+A + G + +S FL V E P PS ++ +S + + W P G SP+ Y Q +EG W+ Y+ ++T V E S G L P T ++ V + N G PS + F PP+A +++ R Y+R +D+ + E++ + H + SS + +T+ GG T++ + DLRP T+Y V+++ +N + + +V+T E VP+ P V+ + + T+ I W +P +G + G+K+ L ++ G +A L T + S + + ++ L + +Y V+ AY G G + + + D+
Sbjct: 7 PRNKXAVSGGDVLLLCPVSGYPIERIVWSKEGRELRGPRPTPLSNGTLLIQKVEASDATTYICVVQNRVGQRAQGKIHLLAIKAPKIAPFHFAPDLKEDDRAQVVCSITSGDLPIQISWEKDGKVLEPDSNIQIQNNVFGSSLLFFRLKAQHSGFYKCIASNAAETTNFTSQLVVKVPPSWLLAPKDIVTRHRSQVVLDCQASGNPRPTVSWRRAHSSSYSGNHISRDKDGSYYEYVQTSEPGRSFHPNGSLILHHVTPEDEGQYRCEVQNGVGAISKSVFVTVQV-----PVYFGTGSQNRTAVAGKDASLVCNALGNSPINLEW-KTGLREKIGARMTSRYSIVEANSSGGVLSTLTIRSVRRSDSGEFICEAGNDFGTSSISIFLSVV---------------ESPEPPSSVHITELSSRSINLAW-YPAYDGNSPVLQYIVQYKEGGVHWR------------------------YHHNKT----VGGGE---STGIKLGGLRPYTEYNMRVMSVNEVGAGDPSEVITAVTLQEDIANYDYKAVFMYPPEA--SLANGRILGYYVRYGATDESLDEIRHQQ-----------------------HTNPNNGLISSSSPNLARMTVRGGRTQA-LVGDLRPFTKYQVYVVAFNEKGLSPHSTKTIVSTLEGVPSGPPLKVRCEAESSTSLSIAWRQPEVAHRNGIILGYKISYRLTNVVGQDAGKSDLIRTKKTSDDRISIHQLNKFSSYYVKXRAYXAVGDGYSSEKIICKTGADV 707 HSP 2 Score: 56.6102 bits (135), Expect = 3.146e-8 Identity = 73/302 (24.17%), Postives = 114/302 (37.75%), Query Frame = 0
Query: 309 KHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAG-FVEASAHLQVFSPPV---FLVKPTDQKGVQGSTVQIPCVAAGNPVPI-LFWMKEGGSGVLLPGSN---QGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEIS--------------------DSALYTCQAKSTSGQAMLSAFLRV 580
+ P N AV+G V C V G P I WS+ L R L + + D Y C+ +NR G + HL P F P D K + Q+ C +PI + W K+G VL P SN Q +V S R+ + SG Y C + + + +S++ ++ PP + P + R S C+A+ P+ V W + S+ + I D D + ++E ++ S D Y C+ ++ G S F+ V
Sbjct: 5 RXPRNKXAVSGGDVLLLCPVSGYPIERIVWSKEGRELRGPRPTPLSNGTLLIQKVEASDATTYICVVQNRVGQRAQGKIHLLAIKAPKIAPFHFAP-DLK--EDDRAQVVCSITSGDLPIQISWEKDG--KVLEPDSNIQIQNNVFGSSLLFFRL---KAQHSGFYKCIASNAAETTNFTSQLVVKV-----PPSWLLAPKDIVTRHRSQVVLDCQASGNPRPTVSWRR--AHSSSYSGNHISRDKDGS-YYEYVQTSEPGRSFHPNGSLILHHVTPEDEGQYRCEVQNGVGAISKSVFVTV 290
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000008452 (supercontig:LSalAtl2s:LSalAtl2s518:328679:339984:1 gene:EMLSAG00000008452 transcript:EMLSAT00000008452 description:"maker-LSalAtl2s518-augustus-gene-2.23") HSP 1 Score: 111.694 bits (278), Expect = 5.419e-25 Identity = 137/577 (23.74%), Postives = 220/577 (38.13%), Query Frame = 0
Query: 21 PVITEHPQNS-----SVTRNEPVTLRCEAQGDPEPQIKWFKD---GEPVVTAAAD-HKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGN-SPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVA--CNREDFDCPKGLEAIM------GNFGR-QLIRLGKRPEARFLLSIEGEG------------TLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPA----------QRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-PPVFLVKPTDQK--GVQGSTVQIPCVAAGNPVPILFWMKEG---GSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQD-----RPPPIIQIGPVNQTQRIGSSTNFPCEATPQTRVEWLKDGVPISTMNDPRIKVDHD 545
PV + P + S + ++L C A G P P W+ + + D K R+ + G L D G Y+C+A+N FG +RSR+ ++ + + + G I G +I C PP+ P +W +D +E D I + GN S +K E + S+ S T P L V+ N + P+ I G + R + I G P A + + +G LII +V+ +D+G+Y C A N + +SA +LS+Q P ++ D V + C G P + +W + +L R D L + + D+ +Y CLA N +S L+V S P F P ++K +G + I C G P P W K G GSG G + G L I + DS V+TC + +E G ++ + ++ +P P++ + GSS N C+A L D ++N+ +I+ D
Sbjct: 278 PVFIKQPNQTIFDVGSRSVKNDISLLCIAHGWPTPTYSWYSEFFSNDSYTEQKVDPLKDSRITISGGQLII---NNPNSVTDRGKYFCQASNKFGTIRSRSVTIAFGFIGEFILRRNYETGDINWGKSI-SCDPPQYFPLVKFYWNRDYFPNYVEEDRRI--MVSYDGNLYFSSVEKIDEGNYSC--------SVQSSVASSGRTGPFFRLKVSPHPNYQQLRFPQNFPKIFPEAPVAGQYVRMECISFG-YPVAHYNWTRKGSDIPKDALVTNHNRVLIIPRVKVEDQGEYICRAYN-DKLSISASVKLSIQSRPVFTISIGDLHVDEKDDVTWTCEAFGIPDVKYKWLKNAKLLKTGDDKLAIEDRGRYEIKDNV-LIIRQVSKRDQGMYECLAYNDLDARYSSGQLRVLSFSPSFDKYPLEEKLFAAEGGNITIKCKPEGAPTPEFVWRKNGAKIGSG--------GKYIIYKSGSLFIRRVNADDSAVFTCEASNEYGRAESTGRLIVKRGPSFQAGIKPKPLVIVNK-------GSSVNLKCKAVADP----LLDMAYSWSLNNLKIRFSED 818 HSP 2 Score: 92.8189 bits (229), Expect = 3.289e-19 Identity = 81/334 (24.25%), Postives = 138/334 (41.32%), Query Frame = 0
Query: 260 LSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPY----VLKHPENV------SAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQ------GHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT---SQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQA 572
+ + +G L S V+ DEG Y C ++ L+V P+ L+ P+N + VAG VR C G P W+R+ +P + T L + + ED+ Y C A N + AS L + S PVF + D + V C A G P W+K + +L G ++ G + + +L I S++D G+Y C + ++ A +S ++++ + S D+ P ++ G + C E P W K+G I + + + +L + DSA++TC+A + G+A
Sbjct: 433 IMVSYDGNLYFSSVEKIDEGNYSCSVQSSVASSGRTGPFFRLKVSPHPNYQQLRFPQNFPKIFPEAPVAGQYVRMECISFGYPVAHYNWTRKGSDIPKDALVTNHNRVLIIPRVKVEDQGEYICRAYNDKLSISASVKLSIQSRPVFTISIGDLHVDEKDDVTWTCEAFGIPDVKYKWLK--NAKLLKTGDDKLAIEDRGRYEIK-DNVLIIRQVSKRDQGMYECLAYNDLDARYSSGQLRVLSFSPSFDKYPLEEKLFAAE-----GGNITIKCKPEGAPTPEFVWRKNGAKIGSGG--KYIIYKSGSLFIRRVNADDSAVFTCEASNEYGRA 756 HSP 3 Score: 72.4034 bits (176), Expect = 5.365e-13 Identity = 79/294 (26.87%), Postives = 110/294 (37.41%), Query Frame = 0
Query: 260 LSIEGEG-------TLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHP--ENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG--MLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFL--VKPTDQKGV-QGSTVQIPCVAAGNP------------------------------------VPILFWMKE-GGSGVLLPGSNQGHVHVSP----------------EGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ 486
L+IE G LII QV +D+G Y+C+A N R S+ L P K+P E + A G + C +G P PE W R+NG + + L + + +D V+TC A N G E++ L V P F +KP V +GS+V + C A +P + L W+ E LL S Q H S +G L+I N S ++G Y C S G I A S+V I
Sbjct: 624 LAIEDRGRYEIKDNVLIIRQVSKRDQGMYECLAYNDLDARYSSGQLRVLSFSPSFDKYPLEEKLFAAEGGNITIKCKPEGAPTPEFVW-RKNGAKIGSGGKYIIYKSGSLFIRRVNADDSAVFTCEASNEYGRAESTGRLIVKRGPSFQAGIKPKPLVIVNKGSSVNLKCKAVADPLLDMAYSWSLNNLKIRFSEDEENYRLLSLQNAEGQRIGGLSWLSELSDHQKLLQSSGQFLNHHSSYTKGTGNFNKFRRGNRDGYLKIYNISYAEAGKYICIVDSAVGRITAESQVIIH 916 HSP 4 Score: 61.2326 bits (147), Expect = 1.534e-9 Identity = 84/356 (23.60%), Postives = 148/356 (41.57%), Query Frame = 0
Query: 260 LSIEGEGTLIISQVQP-KDEGQYQCVAKNVAGIRLSAKARLSLQVL-PYVLKHPENVSAVAGDTVRFGCAVKGRP---HPEIR--WSRR---NGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAEN------RAG---FVEASAH--LQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGS---GVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDH------DNTLHFEELEISDSALYTC------QAKSTSGQAMLSAF 577
++I G G LII+ D G+Y C A N G S ++ + ++L+ + GD + +G ++ P P ++ W+R N + +R+ + L ++ DE Y+C ++ R G ++ S H Q P K + V G V++ C++ G PV W ++G L+ N+ +L I +D G Y C + ++ +I AS ++ IQ+ RP I IG ++ ++ + CEA P + +WLK+ + T +D D DN L ++ D +Y C A+ +SGQ + +F
Sbjct: 339 ITISG-GQLIINNPNSVTDRGKYFCQASNKFGTIRSRSVTIAFGFIGEFILRR----NYETGD-INWGKSISCDPPQYFPLVKFYWNRDYFPNYVEEDRRIMVSYDGNLYFSSVEKIDEGNYSCSVQSSVASSGRTGPFFRLKVSPHPNYQQLRFPQNFPKIFPEAPVAGQYVRMECISFGYPVAHYNWTRKGSDIPKDALVTNHNR---------VLIIPRVKVEDQGEYICRAYNDKLSISASVKLSIQS---RPVFTISIGDLHVDEK--DDVTWTCEAFGIPDVKYKWLKNAKLLKTGDDKLAIEDRGRYEIKDNVLIIRQVSKRDQGMYECLAYNDLDARYSSGQLRVLSF 674 HSP 5 Score: 54.299 bits (129), Expect = 1.889e-7 Identity = 45/152 (29.61%), Postives = 72/152 (47.37%), Query Frame = 0
Query: 19 SPPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVT-----AAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLK-----DKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDG 160
S PV T + V + VT CEA G P+ + KW K+ + + T A D + + ++ +R V + D G+Y C A N +R +S + VL DK+ +E + + A +GG +C P+G+PTP W+K+G
Sbjct: 575 SRPVFTISIGDLHVDEKDDVTWTCEAFGIPDVKYKWLKNAKLLKTGDDKLAIEDRGRYEI---KDNVLIIRQVSKR---DQGMYECLAYNDLD---ARYSSGQLRVLSFSPSFDKYPLE-EKLFAAEGGNITIKC-KPEGAPTPEFVWRKNG 715
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000003860 (supercontig:LSalAtl2s:LSalAtl2s209:184550:379550:-1 gene:EMLSAG00000003860 transcript:EMLSAT00000003860 description:"maker-LSalAtl2s209-augustus-gene-4.12") HSP 1 Score: 103.99 bits (258), Expect = 1.072e-22 Identity = 92/323 (28.48%), Postives = 147/323 (45.51%), Query Frame = 0
Query: 271 SQVQPKDEGQYQCVA--KNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRN--------GMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGN-TSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
S P+DEG YQCVA K + I +S A L ++ P P ++ G T F C ++ + E S N ++ R+ L ++N+ P D Y+C+ E V SPP F+VKP ++ +QG+T+ + C A G P P + W+K+G S L + +G L+I + +E DSG Y C + + + ++ V++ ++ P + P NQ F C ++ P +V+W K+G I T + +I VD N L L SD+ +Y C A ++ G SA L+V
Sbjct: 80 SLTDPRDEGIYQCVAXVKGLGKI-VSKTAVLIVKDPPSFELEPSDLQVFLGQTAAFQCLLETPDNSEDELSSDNIQRLKDDQPLILNHRISIKPSGMLEIINVEPSDRAKYSCVYGE-----ELKTSSLVNSPPNFIVKPRNRAVLQGTTLILDCAANGVPQPEITWLKDGAS--LDMSHLDSRLTKLGKGSLKIQDLNAETDSGAYQCRAENTEDSQDVTAIVEVHSA-----PEFVVRPSNQMCSAKGDITFECKVQSFPPAKVQWYKNGELIVTSDYFQI-VDGSN-LRILGLLDSDAGMYQCMATNSVGNVQSSAQLKV 387 HSP 2 Score: 90.1225 bits (222), Expect = 1.794e-18 Identity = 104/401 (25.94%), Postives = 159/401 (39.65%), Query Frame = 0
Query: 23 ITEHPQNSSVTRNEPVTLRCEAQGDPEP---QIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKE-NDGGIYWCEA-TNTFGRVRSRNASLVVAVLKD--KFRIEPQDVGAIQGGTAIFEC--GPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSE-------EQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCP-------------KGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPK-DEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG--MLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVF 391
T+ P + + NE L C A + KW ++G D R LL +GSL F S + D GIY C A G++ S+ A L+V KD F +EP D+ G TA F+C P S S I L+ D + +I PS E ++ K +E+ T SL S P +P+ V+ DC G M + +L +LGK G+L I + + D G YQC A+N + A + + P + P N A + F C V+ P +++W +NG ++ + DG+ L ++ ++ D +Y C+A N G V++SA L+V
Sbjct: 14 FTKEPSDIFMKENESGLLLCSASTTSTASPVEYKWKRNGH--YLQYPDFNGRRRLLSNGSLHFSWIYNSLTDPRDEGIYQCVAXVKGLGKIVSKTAVLIV---KDPPSFELEPSDLQVFLGQTAAFQCLLETPDNSEDELS----SDNIQRLKDDQPLILNHRISIK-PSGMLEIINVEPSDRAKYSCVYGEELKTSSLVNS-PPNFIVKPRNRAVLQGTTLILDCAANGVPQPEITWLKDGASLDMSHLDSRLTKLGK-------------GSLKIQDLNAETDSGAYQCRAENTEDSQ-DVTAIVEVHSAPEFVVRPSNQMCSAKGDITFECKVQSFPPAKVQW-YKNGELIVTSDYFQIVDGSNLRILGLLDSDAGMYQCMATNSVGNVQSSAQLKVI 388 HSP 3 Score: 81.6481 bits (200), Expect = 7.571e-16 Identity = 60/231 (25.97%), Postives = 99/231 (42.86%), Query Frame = 0
Query: 260 LSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPA----QRVHTTDGTQLTLMNIVPE-DEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKI 485
+SI+ G L I V+P D +Y C V G L + ++ P + P N + + G T+ CA G P PEI W + L R+ L + ++ E D Y C AEN + +A ++V S P F+V+P++Q + C P + W K G V + + LRI + D+G+Y C + + G + +S+++K+
Sbjct: 168 ISIKPSGMLEIINVEPSDRAKYSC----VYGEELKTSSLVNSP--PNFIVKPRNRAVLQGTTLILDCAANGVPQPEITWLKDGASLDMSHLDSRLTKLGKGSLKIQDLNAETDSGAYQCRAENTEDSQDVTAIVEVHSAPEFVVRPSNQMCSAKGDITFECKVQSFPPAKVQWYKNGELIV-----TSDYFQIVDGSNLRILGLLDSDAGMYQCMATNSVGNVQSSAQLKV 387 HSP 4 Score: 61.2326 bits (147), Expect = 1.352e-9 Identity = 34/125 (27.20%), Postives = 63/125 (50.40%), Query Frame = 0
Query: 805 HTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGT-EALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGP 928
+ LTDLR T+Y+ ++ N +G ++ + T D+P P NV ++ + + +++W PP+ +G + G+K+ G ++ FT + S ++ L G Y V++AA G GP
Sbjct: 575 YHLTDLREYTDYTFWVSASNANGEGVHSEEIKCMTYSDIPADPPTNVTLEAASSRSILLRWEPPPKESQNGIISGYKLRYKKKGRGKSEIFTTDASRRLFVIPKLFVGTEYQVKIAAMTVNGTGP 699 HSP 5 Score: 57.3806 bits (137), Expect = 1.981e-8 Identity = 44/146 (30.14%), Postives = 63/146 (43.15%), Query Frame = 0
Query: 19 SPPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKD-KFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRIL 163
SPP P+N +V + + L C A G P+P+I W KDG + + D S L GS L+ E D G Y C A NT S++ + +V V +F + P + G FEC P W K+G ++
Sbjct: 205 SPPNFIVKPRNRAVLQGTTLILDCAANGVPQPEITWLKDGASLDMSHLD--SRLTKLGKGS---LKIQDLNAETDSGAYQCRAENT---EDSQDVTAIVEVHSAPEFVVRPSNQMCSAKGDITFECKVQSFPPAKVQ-WYKNGELI 341
BLAST of roundabout 1 vs. SwissProt
Match: gi|38372441|sp|Q810U4.2|NRCAM_MOUSE (RecName: Full=Neuronal cell adhesion molecule; Short=Nr-CAM; AltName: Full=Neuronal surface protein Bravo; Short=mBravo; AltName: Full=NgCAM-related cell adhesion molecule; Short=Ng-CAM-related; Flags: Precursor) HSP 1 Score: 158.688 bits (400), Expect = 3.675e-38 Identity = 233/983 (23.70%), Postives = 378/983 (38.45%), Query Frame = 0
Query: 20 PPVITEH-PQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDG-------EPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVV----AVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGN----SPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNRED---------------FDCPKGLEA----IMGN-FGRQLIRLGKRPEARFLLSIEG------------EGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGM-------LPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPP--VFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC----EATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLR-VAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTS-RTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS--DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLI---------DINGTEALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGP 928
PP IT+ P++ + E + ++CEA+G P P W ++G +P+VT SG+L + K E G+Y C A N G S N +VV + L K R+EP + G + + C PP G P FW D L + Q + G+ + E ++ + AR + T K+ + ++ +A N D F P+G E+ + GN + I G + + +G + TL I+ V D G YQC+AKN G + ++++ PY + P+N+ G+ C G P P I W NG+ P++++ DG + N+ VY C A N+ G++ A+A + V + P + T + + + C G+P+P + W K G G L ++ + G L I + +G YTC + ++ G +A +EV ++ P II+ QR GS +F C + T + WLKD + ND R D D+ L +++ D YTC A +T A SA LR VAP P + P+ P+ + T NQL DK +++ +P PI + F IEY + W EV ++ P V A N G S PS S+ + T KA E +P + + D V+ K + S L +++ QK D D + + ++ + ++ Y + + N VM + ED+P AP NV++ ++N T A + W P + + G L+G+++ + E T + + +L L YA+ V N +G GP
Sbjct: 39 PPTITQQSPKDYIIDPRENIVIQCEAKGKPPPSFSWTRNGTHFDIDKDPLVTMKPG---------SGTLVINIMSEGKAETYEGVYQCTARNERGAAVSNN--IVVRPSRSPLWTKERLEP--IVLQNGQSLVLPCRPPIGLPPAIIFWM-DNSFQRLPQSERVS--QGLNGDLYFSNVLPEDTREDYICYARFNHTQTIQQKQPISLKVISVDELNDTIAANLSDTEFYGAKSSKERPPTFLTPEGNESHKEELRGNVLSLECIAEGLPTPIIYWIKEDGMLPANRTFYRNFKKTLQITHVSEADSGNYQCIAKNALGA-VHHTISVTVKAAPYWIVAPQNLVLSPGENGTLICRANGNPKPRISW-LTNGVPIEIALDDPSRKI---DGDTIIFSNVQESSSAVYQCNASNKYGYLLANAFVNVLAEPPRILTSANTLYQVIANRPALLDCAFFGSPMPTIEWFK-GTKGSAL---HEDIYVLHDNGTLEIPVAQKDSTGTYTCVARNKLG--MAKNEVHLEIKD--PTRIIKQPEYAVVQR-GSKVSFECRVKHDHTLIPTIMWLKDNGELP--NDERFSTDKDH-LVVSDVKDDDGGTYTCTANTTLDSASASAVLRVVAP---------TPTPAPIYDVPNPPFDLELT-NQL----DKSVQLTWTPGDDNN-------------------SPI----TKFIIEYEDAMHDAGLWRHQAEVSGTQTTAQLKLSPYVNYSFRVMAENSIGRSMPSEASEQYLT--KAAEPDQNPMAVEGLGTEPDNLVITWKPLNGFQSNGPGLQYKVSWRQK---------------DGDDEWTSVVVA--NVSKYIVSGTPTFVPYLIKVQALNDVGFAPEPAAVMGHSGEDLPMVAPGNVRVSVVNSTLAEVHWDPVPPKSVRGHLQGYRIYYWKTQSSSKRNRRHIEKKILTFQGTKTHGMLPGLQPYSHYALNVRVVNGKGEGP 932
BLAST of roundabout 1 vs. SwissProt
Match: gi|341941778|sp|Q3UQ28.2|PXDN_MOUSE (RecName: Full=Peroxidasin homolog; Flags: Precursor) HSP 1 Score: 158.688 bits (400), Expect = 4.813e-38 Identity = 130/438 (29.68%), Postives = 204/438 (46.58%), Query Frame = 0
Query: 165 LELDPSICWVQKI------QGNSPSEEQKEKEKLFEARK-DEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARF----LLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKAR-LSLQVL-----PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGM-LPAQ-RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ-TSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
L D I W+ + GN+ + E + + R I E L P++ + PQ V + N F C GN ++I L E L++ +GTL+I Q DEG YQC+AKNVAG AK + ++L+ L P + P+N + G++V C+ G P P+I W+R + LP RV+ T L + N+ D YTC A N + A+A + V + P F V P + ++G TV C A G+P P++ W K GGS + + ++ H+ +S G LRI + D G Y C +V+ I+ S +V T Q R P+ P + T +G++ PC + P+ + W KDGV ++ + + + L ++ +D+ Y C A++T G A +S L V
Sbjct: 191 LHCDCEILWLADLLKTYAQSGNAQAAATCEYPRRIQGRSVATITPEELNCER-PRITSEPQDADVTSGNTVYFTC-----RAEGNPKPEIIWLRNNNELSMKTDSRLNLLDDGTLMIQNTQEADEGVYQCMAKNVAG---EAKTQEVTLRYLGSPARPTFVIQPQNTEVLVGESVTLECSATGHPLPQITWTRGDRTPLPIDPRVNITPSGGLYIQNVAQSDSGEYTCFASNSVDSIHATAFIIVQALPQFTVTPQSRVVIEGQTVDFQCAAKGHPQPVIAWTK-GGSQLSV---DRRHLVLS-SGTLRISGVALHDQGQYECQAVN----IIGSQKVVAHLTVQPRVTPVFASIPSDMTVEVGTNVQLPCSSQGEPEPAITWNKDGVQVT--ESGKFHISPEGFLTINDVGTADAGRYECVARNTIGYASVSMVLSV 608 HSP 2 Score: 122.865 bits (307), Expect = 4.710e-27 Identity = 107/391 (27.37%), Postives = 171/391 (43.73%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASL--VVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSI-------CWVQKIQGNSPSEEQKEKEKLFEARKDEI-GTESLTLSYCPKLDTRPQIELVVACNREDFDC-PKG--LEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQ--VLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRN-GMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPV 395
P IT PQ++ VT V C A+G+P+P+I W ++ + + S LL G+L +Q+ +E D G+Y C A N G +++ +L + + + F I+PQ+ + G + EC G P P W + R L +DP + ++Q N + E D I T + + P+ PQ +V+ DF C KG I G + + +R +LS GTL IS V D+GQY+C A N+ G + A L++Q V P P +++ G V+ C+ +G P P I W++ + + + H + LT+ ++ D Y C+A N G+ S L V P V
Sbjct: 243 PRITSEPQDADVTSGNTVYFTCRAEGNPKPEIIWLRNNNEL---SMKTDSRLNLLDDGTLM----IQNTQEADEGVYQCMAKNVAGEAKTQEVTLRYLGSPARPTFVIQPQNTEVLVGESVTLECS-ATGHPLPQITWTRGDRT-PLPIDPRVNITPSGGLYIQ----NVAQSDSGEYTCFASNSVDSIHATAFIIVQALPQFTVTPQSRVVIEGQTVDFQCAAKGHPQPVIAWTKGGSQLSVDRR---HLVLS---SGTLRISGVALHDQGQYECQAVNIIGSQ-KVVAHLTVQPRVTPVFASIPSDMTVEVGTNVQLPCSSQGEPEPAITWNKDGVQVTESGKFHISPEGFLTINDVGTADAGRYECVARNTIGYASVSMVLSVNVPDV 613
BLAST of roundabout 1 vs. SwissProt
Match: gi|12643619|sp|O60469.2|DSCAM_HUMAN (RecName: Full=Down syndrome cell adhesion molecule; AltName: Full=CHD2; Flags: Precursor) HSP 1 Score: 155.221 bits (391), Expect = 6.036e-37 Identity = 223/952 (23.42%), Postives = 381/952 (40.02%), Query Frame = 0
Query: 17 AASPPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWV--QKIQGNSPSEEQKEKEKLFEARKDEI------------GTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGE--GTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLP---AQRVHTTDGTQLTLMNIVPE-DEDVYTC--LAENRAGFVEASAHLQVFSPPVFLVKPTD-QKGVQGSTVQIPCVAAGNPVPI-LFWMKEGGSGVLLPGSNQGHV-HVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEW-LKDGVPISTMN----DPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAM-LSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKF-VTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKV---LIDINGTEALN-FTLEPSSNSLI--LYNLTEGLTYAVQVAAYNRQGVGP 928
A S P I + + + V L C+A G PEP +W KD P+ + K+ LL +++ + +D G Y CE +N +G + V LK I P+ V + G C G+ W ++G IL + I + + + + + + F RKD++ GT + ++ K+ P + + CN + P I I G +++ EG L IS Q +D G Y+C A N AG+ L +AR++++ P ++ +N++A+AG C V G P+ I+W + + +LP Q +GT L L ++ E DE YTC L + + S H+ V PP ++P + + G V IPCV +PI + W K+G +PGS + ++ LRI N S +G YTC + +E+ A+ S++ + R PP + P +Q G + C E P + W G + + RI+V + +L + + DS Y C+ + G + S +L V + + ++ E +++ W+K RI + Y V T ++ + ++ S+ QI +P V+ GF + H + G+ I Q PP E +E+K+VK +R + L W + +TGY I+ ++ S DS DS S T+ D+ P++ YS+ M N K P+ + +T E P P+ V ++ ++ + + W P + +G ++G+++ G N +++ S +S + L NL + Y + V A NR G GP
Sbjct: 221 ANSAPSILDGFDHRKAMAGQRVELPCKALGHPEPDYRWLKDNMPLELSGRFQKTVTGLL----------IENIRPSDSGSYVCEVSNRYGTAKVIGRLYVKQPLKAT--ISPRKVKSSVGSQVSLSCSV-TGTEDQELSWYRNGEILNPGKNVRITGINHENLIMDHMVKSDGGAYQCF-VRKDKLSAQDYVQVVLEDGTPKIISAFSEKV-VSPAEPVSLMCNVKGTPLP----TITWTLDDDPILKGGSHRISQMITSEGNVVSYLNISSSQVRDGGVYRCTANNSAGVVL-YQARINVRG-PASIRPMKNITAIAGRDTYIHCRVIGYPYYSIKWYKNSNLLPFNHRQVAFENNGT-LKLSDVQKEVDEGEYTCNVLVQPQLS-TSQSVHVTVKVPP--FIQPFEFPRFSIGQRVFIPCVVVSGDLPITITWQKDGRP---IPGSLGVTIDNIDFTSSLRISNLSLMHNGNYTCIARNEAAAVEHQSQLIV-----RVPPKFVVQPRDQDGIYGKAVILNCSAEGYPVPTIVWKFSKGAGVPQFQPIALNGRIQVLSNGSLLIKHVVEEDSGYYLCKVSNDVGADVSKSMYLTVKIPAMITSYPNTTLATQ--GQKKEMSCTAHGEKPIIVRWEKEDRIINPEMARYLVS------------TKEVGEEVI---STLQI-------------LPTVREDSGF--------FSCHAINSYGEDRGI-----IQLTVQEPPDPPE-----------------IEIKDVK---ARTITLRWTMGFDGNSPITGYDIECKNKS--DSWDSAQRTKDVSPQLNSATIIDIHPSSTYSIRMYAKNRIGKSEPSNELTITADEAAPDGPPQEVHLEPISSQSIRVTWKAPKKHLQNGIIRGYQIGYREYSTGGNFQFNIISVDTSGDSEVYTLDNLNKFTQYGLVVQACNRAGTGP 1074 HSP 2 Score: 145.591 bits (366), Expect = 6.138e-34 Identity = 178/715 (24.90%), Postives = 296/715 (41.40%), Query Frame = 0
Query: 260 LSIEGEGTLIISQVQPK-DEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHP-EIRWSRRNGMLPAQRVHTTDGTQLT----LMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGV--LLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASS---EVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQT--RVEWLKDGVPIS------TMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQK-----FVTGYHIQLRDISDLDSSDSYDVITI-SGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKV-----LIDINGTEALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHP 944
++ E GTL +S VQ + DEG+Y C + S ++++V P++ E G V C V P I W + +P T D T + N+ YTC+A N A VE + L V PP F+V+P DQ G+ G V + C A G PVP + W G+GV P + G + V G L I + E+DSG Y C ++ GA ++ S VKI P +I P G C A + V W K+ I+ ++ + + +TL DS ++C A ++ G+ + + + P P E + + + + + W G SPI Y + + S WD S T V+ P++ S+ T+ D P++T + + A+N G S PS ++ T +A D P+ + ++ + S+ ++++W+ P+K + GY I R+ S + +++I++ + G + +TL +L T+Y + + N G ++ ++ TT EDVP+ PENV+ + + I W + L+G L+GF+V L+D E N T + SL L L + Y++QV A+ R G G + + + D+ P
Sbjct: 551 VAFENNGTLKLSDVQKEVDEGEYTCNVLVQPQLSTSQSVHVTVKVPPFIQPF-EFPRFSIGQRVFIPCVVVSGDLPITITWQKDGRPIPGSLGVTIDNIDFTSSLRISNLSLMHNGNYTCIARNEAAAVEHQSQLIVRVPPKFVVQPRDQDGIYGKAVILNCSAEGYPVPTIVWKFSKGAGVPQFQPIALNGRIQVLSNGSLLIKHVVEEDSGYYLCKVSNDVGADVSKSMYLTVKI-------PAMITSYPNTTLATQGQKKEMSCTAHGEKPIIVRWEKEDRIINPEMARYLVSTKEVGEEVISTLQILPTVREDSGFFSCHAINSYGE----------------DRGIIQLTVQEPPDPPEIEIKDVKARTITLRWTMGFD-GNSPITGYDIECKNKSDS---------WD---------------SAQRTKDVS-PQLNSA---TIIDIHPSSTYSIRMYAKNRIGKSEPS--NELTITADEAAPD-GPPQEV-------------HLEPISSQSIRVTWKA--PKKHLQNGIIRGYQIGYREYS-TGGNFQFNIISVDTSGDSEVYTLDNLNKFTQYGLVVQACNRAGTGPSSQEIITTTLEDVPSYPPENVQAIATSPESISISWSTLSKEALNGILQGFRVIYWANLMDGELGEIKNITT--TQPSLELDGLEKYTNYSIQVLAFTRAGDGVRSEQIFTRTKEDVPGP 1191 HSP 3 Score: 87.0409 bits (214), Expect = 6.060e-16 Identity = 87/341 (25.51%), Postives = 134/341 (39.30%), Query Frame = 0
Query: 256 ARFLLSIEGEGTLIISQVQPKDE-GQYQCVAKN--VAGIRLSAKARLSL----QVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGP---VNQTQRIGSSTNFPCEATPQTRVEWLKDGVPISTMNDPRIK---VDHDNTLHFEEL---EISDSALYTCQAKSTSGQAMLSAFLRV 580
+RFL I G L I VQ +D Y+C+ ++ R S ARL + P +L ++ A+AG V C G P P+ RW + N L T L + NI P D Y C NR G + L V P + P K GS V + C G L W + G +L PG N ++ E ++ + + + D G Y C + L++ + +D P II V+ + + N + TP + W D PI RI N + + + ++ D +Y C A +++G + A + V
Sbjct: 171 SRFL--ITSTGALYIKDVQNEDGLYNYRCITRHRYTGETRQSNSARLFVSDPANSAPSILDGFDHRKAMAGQRVELPCKALGHPEPDYRWLKDNMPLELSGRFQKTVTGLLIENIRPSDSGSYVCEVSNRYGTAKVIGRLYVKQPLKATISPRKVKSSVGSQVSLSCSVTGTEDQELSWYRNG--EILNPGKNVRITGINHENLI-MDHMVKSDGGAYQCFVRKDK---LSAQDYVQVVLEDGTPKIISAFSEKVVSPAEPVSLMCN--VKGTPLPTITWTLDDDPILKGGSHRISQMITSEGNVVSYLNISSSQVRDGGVYRCTANNSAGVVLYQARINV 501
BLAST of roundabout 1 vs. SwissProt
Match: gi|172044151|sp|A4IGL7.1|PXDN_XENTR (RecName: Full=Peroxidasin; Flags: Precursor) HSP 1 Score: 154.836 bits (390), Expect = 7.363e-37 Identity = 118/433 (27.25%), Postives = 195/433 (45.03%), Query Frame = 0
Query: 165 LELDPSICWVQKI------QGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARF----LLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQ---VLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGM-LPAQ-RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
L D I W+ + GN+ + E + + R T S P++ + PQ V N F C GN ++I L E L++ +GTL+I + D+G YQC+AKNVAG + + L P + P+N + G++V C+ G+PHP + W+R + LP+ R++ T L + N+ +D YTC A N + ++A++ V + P F V P D+ +G TV C A GNP P++ W K GG+ + + +Q V G LRI + D G Y C +V+ G+ ++++ +QT R P+ P + T +G+ PC + P + W KDG+ ++ + + L + ++D Y C A++ G + +S L V
Sbjct: 184 LHCDCEILWLADLLKIYSESGNAQAAATCEYPRRLQGRSVSTITPSELNCERPRITSEPQDVDVTFGNTVYFTC-----RAEGNPKPEIIWLRNNNELSMKDDSRLNLLNDGTLMIQNTKETDQGIYQCMAKNVAGEVKTHEVTLRYYGTPATPTFVIQPQNTEVLVGESVTLECSATGQPHPRVTWTRGDRTPLPSDPRINITPSGGLYIQNVNQDDAGEYTCFATNSVETIHSTAYIIVQAVPQFTVVPQDRNVFEGHTVDFHCEAQGNPKPVIAWTK-GGNQLSVDRRHQ----VLSSGTLRILRVALHDQGQYECQAVNIVGSKSTAAQLIVQT---RVTPVFATVPNDMTVEVGTDVQIPCSSQGDPLPIITWNKDGIQVT--ESGKFHISPHGYLAIRDAGLADQGRYECVARNPIGYSSVSMVLSV 601 HSP 2 Score: 116.701 bits (291), Expect = 4.449e-25 Identity = 106/392 (27.04%), Postives = 164/392 (41.84%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVV--AVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSI-------CWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDC-----PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQ--VLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRN-GMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPV 395
P IT PQ+ VT V C A+G+P+P+I W ++ + + S LL G+L +Q+ KE D GIY C A N G V++ +L F I+PQ+ + G + EC G P P W + R L DP I ++Q + + E E T + + P+ PQ V + DF C PK + I G + + +R + + GTL I +V D+GQY+C A N+ G + S A+L +Q V P P +++ G V+ C+ +G P P I W++ + + + H + L + + D+ Y C+A N G+ S L V P V
Sbjct: 236 PRITSEPQDVDVTFGNTVYFTCRAEGNPKPEIIWLRNNNEL---SMKDDSRLNLLNDGTLM----IQNTKETDQGIYQCMAKNVAGEVKTHEVTLRYYGTPATPTFVIQPQNTEVLVGESVTLECS-ATGQPHPRVTWTRGDRT-PLPSDPRINITPSGGLYIQNVNQDDAGEYTCFATNSVETIH---STAYIIVQAVPQFTVVPQDRNVFEGHTVDFHCEAQGNPKPV--IAWTKGGNQLSVDRRHQ------VLSSGTLRILRVALHDQGQYECQAVNIVGSK-STAAQLIVQTRVTPVFATVPNDMTVEVGTDVQIPCSSQGDPLPIITWNKDGIQVTESGKFHISPHGYLAIRDAGLADQGRYECVARNPIGYSSVSMVLSVLVPEV 606
BLAST of roundabout 1 vs. SwissProt
Match: gi|172045828|sp|Q92626.2|PXDN_HUMAN (RecName: Full=Peroxidasin homolog; AltName: Full=Melanoma-associated antigen MG50; AltName: Full=Vascular peroxidase 1; AltName: Full=p53-responsive gene 2 protein; Flags: Precursor) HSP 1 Score: 154.836 bits (390), Expect = 7.389e-37 Identity = 129/435 (29.66%), Postives = 199/435 (45.75%), Query Frame = 0
Query: 165 LELDPSICWVQKI------QGNSPSEEQKEKEKLFEARK-DEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARF----LLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSL---QVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGM-LPAQ-RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ-TSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
L D I W+ + GN+ + E + + R I E L P++ + PQ V + N F C GN ++I L E L++ +GTL+I Q D+G YQC+AKNVAG + + L P + P+N + G++V C+ G P P I W+R + LP RV+ T L + N+V D Y C A N V A+A + V + P F V P D+ ++G TV C A GNP P++ W K GGS + + ++ H+ +S G LRI + D G Y C +V+ I+ S +V T Q R P+ P + T +G++ PC + P+ + W KDGV ++ + + + L ++ +D+ Y C A++T G A +S L V
Sbjct: 194 LHCDCEILWLADLLKTYAESGNAQAAAICEYPRRIQGRSVATITPEELNCER-PRITSEPQDADVTSGNTVYFTC-----RAEGNPKPEIIWLRNNNELSMKTDSRLNLLDDGTLMIQNTQETDQGIYQCMAKNVAGEVKTQEVTLRYFGSPARPTFVIQPQNTEVLVGESVTLECSATGHPPPRISWTRGDRTPLPVDPRVNITPSGGLYIQNVVQGDSGEYACSATNNIDSVHATAFIIVQALPQFTVTPQDRVVIEGQTVDFQCEAKGNPPPVIAWTK-GGSQLSV---DRRHLVLS-SGTLRISGVALHDQGQYECQAVN----IIGSQKVVAHLTVQPRVTPVFASIPSDTTVEVGANVQLPCSSQGEPEPAITWNKDGVQVTESG--KFHISPEGFLTINDVGPADAGRYECVARNTIGSASVSMVLSV 611 HSP 2 Score: 124.405 bits (311), Expect = 1.829e-27 Identity = 109/391 (27.88%), Postives = 171/391 (43.73%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVV--AVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSI-------CWVQK-IQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDC-PKGL--EAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQ--VLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRN-GMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPV 395
P IT PQ++ VT V C A+G+P+P+I W ++ + + S LL G+L +Q+ +E D GIY C A N G V+++ +L + + F I+PQ+ + G + EC G P P W + R L +DP + ++Q +QG+S + T + + P+ PQ +V+ DF C KG I G + + +R +LS GTL IS V D+GQY+C A N+ G + A L++Q V P P + + G V+ C+ +G P P I W++ + + + H + LT+ ++ P D Y C+A N G S L V P V
Sbjct: 246 PRITSEPQDADVTSGNTVYFTCRAEGNPKPEIIWLRNNNEL---SMKTDSRLNLLDDGTLM----IQNTQETDQGIYQCMAKNVAGEVKTQEVTLRYFGSPARPTFVIQPQNTEVLVGESVTLECS-ATGHPPPRISWTRGDRT-PLPVDPRVNITPSGGLYIQNVVQGDSGEYACSATNNIDSVH----ATAFIIVQALPQFTVTPQDRVVIEGQTVDFQCEAKGNPPPVIAWTKGGSQLSVDRR---HLVLS---SGTLRISGVALHDQGQYECQAVNIIGSQ-KVVAHLTVQPRVTPVFASIPSDTTVEVGANVQLPCSSQGEPEPAITWNKDGVQVTESGKFHISPEGFLTINDVGPADAGRYECVARNTIGSASVSMVLSVNVPDV 616
BLAST of roundabout 1 vs. nr
Match: gi|108879336|gb|EAT43561.1| (AAEL005011-PA [Aedes aegypti]) HSP 1 Score: 530.02 bits (1364), Expect = 2.718e-166 Identity = 333/993 (33.53%), Postives = 512/993 (51.56%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQ-LTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQD-RPPPIIQIGPVNQTQRIGSSTNFPCEATPQT--RVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEF----DSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSK--LSDKRVMELKEVKILGSRKVKLSWELLIP--QKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDI-NGTEAL-NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAPGMDLV--------VQEVWF-VMVVSFFSVILLVGFLAMVCFRRIRQD 990
P ITEHP + V +N+PVTL C+A+G PEP+I+W+KDGEPV + VLLPSGSLFFLR V SKKE DGG+YWC A+N G V SRNA+L +AVL+D+FR+EP+D G TA+ ECG PKG+P +P+I W RKDEI +EL +DF R GK P AR + ++G G L+IS V+P DEG+YQC+A+N+ G R S +L++QV PY + P +++ + G V+F C V G PHP+I W + NG +P R + + L + N+ +D+ +Y C A N G + A A L V S P F++KP DQ+ C A+GNP P +FW KEG ++ P + G++H+S +G L++ ++D G + C+++S +G+ + + +++ +S D PPP++Q P N T GS PC + T ++ W K+ I+ + PR + TL ++++ DS Y+C A S G+ SAFL+V E+N+ P L P +PS P +N T++ + + W K IGG S Q + +W+ V S + +EYY+ W+ V + T+ + P+T+ +VRA N +G S PSP+S +T A + ++ P L + + + +++EL + + S V+L W+L I ++++ G +++ RD+ +S Y ++TI H +T+L T Y F+ P+ ++G+P+ +V T ED PTAAP N++ MLNLTA ++W PP +G L G+K+ + N ++ L + TL ++ S++L NLT G TY Q+ A+NR G GP P L + D H P + + + E WF V VV +IL + +V F + RQ+
Sbjct: 51 PRITEHPSDVLVPKNDPVTLNCKAEGKPEPEIRWYKDGEPV-----KFTPNHVLLPSGSLFFLRTVHSKKEQDGGVYWCVASNAAGTVNSRNATLQIAVLRDEFRVEPKDTRVAAGETALLECGAPKGNP-----------------EPTISW----------------------RKDEI-----------------MLEL------DDF--------------RPASASGKEP-ARVRI-VDG-GNLLISDVRPTDEGRYQCIAQNMVGSRESGSGKLTVQVKPYFINEPTDITVLVGQRVQFQCTVGGDPHPQILWKKENGHIPVGRAEILEEDRSLVIKNVAQDDQGIYICEAHNSVGQINAKAQLVVNSSPTFIIKPQDQRTNLNGVATFKCTASGNPPPSVFWTKEGSQTLMFPNNTYGNIHISSQGSLQVRGAQKEDDGYFVCSALSVAGSTTSRAYLQVTSSFDSSPPPVLQEVPSNLTLPRGSIAMLPCRGSGPTSPKIYWKKNATDITALG-PRFSIVQGGTLKIDDIQPEDSGWYSCVAYSEKGETSWSAFLQV---EKNLSTMLPRSGSPEL--LPVAPSIPKAVNITNSNVTLMWTK---IGGGGEDSLQEK---------------LWNNPV---SGYLVEYYSPDEKKGWIRAIRVPGNTA-TITNLSPSTSYIFVVRAENAYGYSAPSPLSNLIRT-LSADDGVTMPEELEAARMVLNGKILELVDAVAISSTSVRLEWQLHISSTEEYIEGLYVRYRDLG--ANSQKYSMLTIPNNAAEMHVITNLDKFTRYEFFLTPFFKNVEGQPSNSKVVQTAEDAPTAAPVNIQTGMLNLTAGWVKWSPPPAEHQNGVLLGYKIQVKAGNSSKTLASMTLNSTTTSVMLNNLTTGATYRAQIVAFNRVGAGPYSKPAYLIM--DPAHVIAPPRAHNSLSDIGGYRHHNFMHETWFMVFVVLALLIILTFTVVGVVVFIKKRQN 926
BLAST of roundabout 1 vs. nr
Match: gi|1218261951|ref|XP_021709627.1| (protein sax-3 isoform X2 [Aedes aegypti]) HSP 1 Score: 530.02 bits (1364), Expect = 8.775e-165 Identity = 333/993 (33.53%), Postives = 512/993 (51.56%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQ-LTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQD-RPPPIIQIGPVNQTQRIGSSTNFPCEATPQT--RVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEF----DSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSK--LSDKRVMELKEVKILGSRKVKLSWELLIP--QKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDI-NGTEAL-NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAPGMDLV--------VQEVWF-VMVVSFFSVILLVGFLAMVCFRRIRQD 990
P ITEHP + V +N+PVTL C+A+G PEP+I+W+KDGEPV + VLLPSGSLFFLR V SKKE DGG+YWC A+N G V SRNA+L +AVL+D+FR+EP+D G TA+ ECG PKG+P +P+I W RKDEI +EL +DF R GK P AR + ++G G L+IS V+P DEG+YQC+A+N+ G R S +L++QV PY + P +++ + G V+F C V G PHP+I W + NG +P R + + L + N+ +D+ +Y C A N G + A A L V S P F++KP DQ+ C A+GNP P +FW KEG ++ P + G++H+S +G L++ ++D G + C+++S +G+ + + +++ +S D PPP++Q P N T GS PC + T ++ W K+ I+ + PR + TL ++++ DS Y+C A S G+ SAFL+V E+N+ P L P +PS P +N T++ + + W K IGG S Q + +W+ V S + +EYY+ W+ V + T+ + P+T+ +VRA N +G S PSP+S +T A + ++ P L + + + +++EL + + S V+L W+L I ++++ G +++ RD+ +S Y ++TI H +T+L T Y F+ P+ ++G+P+ +V T ED PTAAP N++ MLNLTA ++W PP +G L G+K+ + N ++ L + TL ++ S++L NLT G TY Q+ A+NR G GP P L + D H P + + + E WF V VV +IL + +V F + RQ+
Sbjct: 26 PRITEHPSDVLVPKNDPVTLNCKAEGKPEPEIRWYKDGEPV-----KFTPNHVLLPSGSLFFLRTVHSKKEQDGGVYWCVASNAAGTVNSRNATLQIAVLRDEFRVEPKDTRVAAGETALLECGAPKGNP-----------------EPTISW----------------------RKDEI-----------------MLEL------DDF--------------RPASASGKEP-ARVRI-VDG-GNLLISDVRPTDEGRYQCIAQNMVGSRESGSGKLTVQVKPYFINEPTDITVLVGQRVQFQCTVGGDPHPQILWKKENGHIPVGRAEILEEDRSLVIKNVAQDDQGIYICEAHNSVGQINAKAQLVVNSSPTFIIKPQDQRTNLNGVATFKCTASGNPPPSVFWTKEGSQTLMFPNNTYGNIHISSQGSLQVRGAQKEDDGYFVCSALSVAGSTTSRAYLQVTSSFDSSPPPVLQEVPSNLTLPRGSIAMLPCRGSGPTSPKIYWKKNATDITALG-PRFSIVQGGTLKIDDIQPEDSGWYSCVAYSEKGETSWSAFLQV---EKNLSTMLPRSGSPEL--LPVAPSIPKAVNITNSNVTLMWTK---IGGGGEDSLQEK---------------LWNNPV---SGYLVEYYSPDEKKGWIRAIRVPGNTA-TITNLSPSTSYIFVVRAENAYGYSAPSPLSNLIRT-LSADDGVTMPEELEAARMVLNGKILELVDAVAISSTSVRLEWQLHISSTEEYIEGLYVRYRDLG--ANSQKYSMLTIPNNAAEMHVITNLDKFTRYEFFLTPFFKNVEGQPSNSKVVQTAEDAPTAAPVNIQTGMLNLTAGWVKWSPPPAEHQNGVLLGYKIQVKAGNSSKTLASMTLNSTTTSVMLNNLTTGATYRAQIVAFNRVGAGPYSKPAYLIM--DPAHVIAPPRAHNSLSDIGGYRHHNFMHETWFMVFVVLALLIILTFTVVGVVVFIKKRQN 901
BLAST of roundabout 1 vs. nr
Match: gi|1218261949|ref|XP_021709626.1| (protein sax-3 isoform X1 [Aedes aegypti]) HSP 1 Score: 529.25 bits (1362), Expect = 7.572e-164 Identity = 333/993 (33.53%), Postives = 512/993 (51.56%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQ-LTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQD-RPPPIIQIGPVNQTQRIGSSTNFPCEATPQT--RVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEF----DSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSK--LSDKRVMELKEVKILGSRKVKLSWELLIP--QKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDI-NGTEAL-NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAPGMDLV--------VQEVWF-VMVVSFFSVILLVGFLAMVCFRRIRQD 990
P ITEHP + V +N+PVTL C+A+G PEP+I+W+KDGEPV + VLLPSGSLFFLR V SKKE DGG+YWC A+N G V SRNA+L +AVL+D+FR+EP+D G TA+ ECG PKG+P +P+I W RKDEI +EL +DF R GK P AR + ++G G L+IS V+P DEG+YQC+A+N+ G R S +L++QV PY + P +++ + G V+F C V G PHP+I W + NG +P R + + L + N+ +D+ +Y C A N G + A A L V S P F++KP DQ+ C A+GNP P +FW KEG ++ P + G++H+S +G L++ ++D G + C+++S +G+ + + +++ +S D PPP++Q P N T GS PC + T ++ W K+ I+ + PR + TL ++++ DS Y+C A S G+ SAFL+V E+N+ P L P +PS P +N T++ + + W K IGG S Q + +W+ V S + +EYY+ W+ V + T+ + P+T+ +VRA N +G S PSP+S +T A + ++ P L + + + +++EL + + S V+L W+L I ++++ G +++ RD+ +S Y ++TI H +T+L T Y F+ P+ ++G+P+ +V T ED PTAAP N++ MLNLTA ++W PP +G L G+K+ + N ++ L + TL ++ S++L NLT G TY Q+ A+NR G GP P L + D H P + + + E WF V VV +IL + +V F + RQ+
Sbjct: 26 PRITEHPSDVLVPKNDPVTLNCKAEGKPEPEIRWYKDGEPV-----KFTPNHVLLPSGSLFFLRTVHSKKEQDGGVYWCVASNAAGTVNSRNATLQIAVLRDEFRVEPKDTRVAAGETALLECGAPKGNP-----------------EPTISW----------------------RKDEI-----------------MLEL------DDF--------------RPASASGKEP-ARVRI-VDG-GNLLISDVRPTDEGRYQCIAQNMVGSRESGSGKLTVQVKPYFINEPTDITVLVGQRVQFQCTVGGDPHPQILWKKENGHIPVGRAEILEEDRSLVIKNVAQDDQGIYICEAHNSVGQINAKAQLVVNSSPTFIIKPQDQRTNLNGVATFKCTASGNPPPSVFWTKEGSQTLMFPNNTYGNIHISSQGSLQVRGAQKEDDGYFVCSALSVAGSTTSRAYLQVTSSFDSSPPPVLQEVPSNLTLPRGSIAMLPCRGSGPTSPKIYWKKNATDITALG-PRFSIVQGGTLKIDDIQPEDSGWYSCVAYSEKGETSWSAFLQV---EKNLSTMLPRSGSPEL--LPVAPSIPKAVNITNSNVTLMWTK---IGGGGEDSLQEK---------------LWNNPV---SGYLVEYYSPDEKKGWIRAIRVPGNTA-TITNLSPSTSYIFVVRAENAYGYSAPSPLSNLIRT-LSADDGVTMPEELEAARMVLNGKILELVDAVAISSTSVRLEWQLHISSTEEYIEGLYVRYRDLG--ANSQKYSMLTIPNNAAEMHVITNLDKFTRYEFFLTPFFKNVEGQPSNSKVVQTAEDAPTAAPVNIQTGMLNLTAGWVKWSPPPAEHQNGVLLGYKIQVKAGNSSKTLASMTLNSTTTSVMLNNLTTGATYRAQIVAFNRVGAGPYSKPAYLIM--DPAHVIAPPRAHNSLSDIGGYRHHNFMHETWFMVFVVLALLIILTFTVVGVVVFIKKRQN 901
BLAST of roundabout 1 vs. nr
Match: gi|752883644|ref|XP_011259531.1| (PREDICTED: protein sax-3 [Camponotus floridanus] >gi|307176930|gb|EFN66247.1| Roundabout-like protein 2 [Camponotus floridanus]) HSP 1 Score: 451.055 bits (1159), Expect = 6.837e-136 Identity = 266/752 (35.37%), Postives = 395/752 (52.53%), Query Frame = 0
Query: 255 EARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG-SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS-DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
EA +++ G L+IS V+P D+G+YQC+A+N+ GI+ SA A L++ V PY + P N + +A T F C V G P PEI W R +G +P R H + L + + P+D+ Y C AEN G + ASA L V S PVF P D+ S V C A G P P +FW +EG ++ PG + Q H V+ G L I KD G Y C+++S++GA A+ +++ + ++ PPPII++GP NQT + S + PC A TP RV W K+GV + RI + + TL ++L +DS LYTC A S SG SA+L V+ F P +S P +PS+P ++NATSN + + W P G S I Y IEYY+S T WV + +T+ D P T LVRA N HGLS P P+S QT + R++ + ++ LKEV+ L S VK+ W++L V G +I+ R++S+ Y ++T+ G S+ LT+L+ T Y F++P+ I+GRP+ + +VTT EDVP+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +LT G Y +VA R G+GP P L ++ QL P P G V++E WF++++ I++V LA + RR + K +GH N
Sbjct: 54 EATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGIKGSAIATLTVHVKPYFVATPSNQTILADQTAEFVCRVGGDPSPEILWRRGDGKMPIGRAHILEDKSLHIERVNPQDQGTYICEAENGVGTISASATLTVHSRPVFSNFPKDETVSSNSDVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQNHYTVTENGSLTIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGG--RITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTVSSG---ASFHRTPDVSALPQNPSKPRIVNATSNSVTLTW-SPGHEGQSKIIGYN------------------------------IEYYSSNLNTGWVVAATGVLDDIYTVTDLRPETNYVFLVRAENNHGLSLPGPLSDVVQTTNVNQHTVPQVELTRARDRLNSEILHLKEVQPLTSTSVKIMWDILGAADLVEGLYIRYREVSE---KPEYQMVTVLNAGATSYVLTNLKKYTRYEFFLVPFYKIIEGRPSNVKLVTTLEDVPSGAPENVHVGMINMTSAFVRWSPPPKNTQNGQLIGYKIQIKSNSSNKILGQMSLNASTTSVIINSLTAGGLYTARVAGQTRIGLGPFSSPTLLNMDPGQI-TQLPPRTDPSHGGASVIRETWFLVLMIMMIFIVIVVLLAALYVRRRQAMSKQLGHLN 765 HSP 2 Score: 178.333 bits (451), Expect = 8.956e-42 Identity = 128/395 (32.41%), Postives = 190/395 (48.10%), Query Frame = 0
Query: 115 LVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQ----KIQGNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLE---AIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHT----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT 487
L VAVL+++FR EPQ+ G TA+ ECGPP+G P PT +WK++G I++LE I V I P+++ K + E + + I T LT+ P P + ++A +F C G + I+ G GK P R I + +L I +V P+D+G Y C A+N G +SA A L++ P P++ + + V F CA +G P P I W+R M P T+ LT+ V +DE Y C A ++AG A+ LQV S PP+ + P +Q S +PC A G P P + W KEG ++ PG G + ++ G L I + DSG+YTC + SESG S+ + + +
Sbjct: 3 LSVAVLREEFRAEPQNTRVAAGETALLECGPPRGHPEPTLYWKRNGHIIDLEATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGIKGSAIAT--LTVHVKPYFVATPSNQTILADQTAEFVCRVGGDPSPEILWRRGD-----GKMPIGR--AHILEDKSLHIERVNPQDQGTYICEAENGVGT-ISASATLTVHSRPVFSNFPKDETVSSNSDVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQNHYTVTENGSLTIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEG--VLVRPG---GRITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTVSS 382 HSP 3 Score: 85.8853 bits (211), Expect = 3.587e-13 Identity = 104/397 (26.20%), Postives = 163/397 (41.06%), Query Frame = 0
Query: 27 PQNSSVTRNEPVTLRCEA-QGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKK-DGRI----LELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGT----ESLTLSYCPKLDTRPQIELVVACNREDFDC-----PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVL-------PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGML-PAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKP 400
PQN+ V E L C +G PEP + W ++G + A + R+ L G + V + D G Y C A N G S A+L V V K F P + + TA F C G P+P W++ DG++ + D S+ I+ +P Q + + EA ++ +GT +LT+ P P+ E V + + F C PK ++L+ G + + ++ G+L I KDEG Y C A + AG ++ A + LQV P + P N + C G P P + W + ++ P R+ L + ++ D +YTC+A + +G SA+L V S F P
Sbjct: 16 PQNTRVAAGETALLECGPPRGHPEPTLYWKRNGHIIDLEA----TKRITLVDGGNLMISDV---RPADQGKYQCIAENMVGIKGSAIATLTVHV-KPYFVATPSNQTILADQTAEFVC-RVGGDPSPEILWRRGDGKMPIGRAHILEDKSL----HIERVNP---QDQGTYICEA-ENGVGTISASATLTVHSRPVFSNFPKDETVSSNSDVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQNHY--TVTENGSLTIKGAVRKDEGHYVCSAISQAG---ASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGGRITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTVSSGASFHRTP 390
BLAST of roundabout 1 vs. nr
Match: gi|1233169468|ref|XP_022186783.1| (roundabout homolog 2-like [Nilaparvata lugens]) HSP 1 Score: 449.514 bits (1155), Expect = 1.389e-135 Identity = 272/800 (34.00%), Postives = 412/800 (51.50%), Query Frame = 0
Query: 221 LVVACNREDFDC-PKGLEAIMGNFGRQLIRLG---KRPEARFLLSIEGE---------------GTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKA-GEDISDPRYL---RSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL-NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHM 994
L VA REDF PK G L+ G PE +G+ G L++++V+ DEG+YQC+ +N+ G+R S A L++ V PY +K P +V+ +A V F C V G P P+I W R +G +P R H D L + ++ PEDE +Y C AEN G V A A L V SPPVF+ KP DQK CVA GNP P +FW KEG ++ PG++ GH+HV+ EG LRI +D+G C+++S +G+ + +++ + D PPPI++IGP NQT I S N PC+A TP ++W KD P+ + +PR+ + TL +L++ DS LYTC A S SG+ SA L V A P S FPS P P ++N T + + I WD +P+ Y V EY++S T WV ++ T+ D +P T+ +VRA N HGLS PS SK +T + G P+ L R +L K V+ L+++ + S VKL WE+L +KFV G +++ RD+S S Y+++T+ G S+T+ +LR T+Y F++P+ ++G+P+ +V T EDVP+A P+N+++ +N TA + W PP + +G + G+K+ I NGT+ + ++ S+ S++L NL V+VAA R G+GP V + + P P P + W V++++F +VILL+ ++ + R+ KH+
Sbjct: 113 LQVAVLREDFRAEPKDTRVAAGETA--LLECGPPKGHPEPTLFWKKDGQVLDLETSNRVRVVDGGNLMMTEVRQADEGRYQCLVQNLVGMRESGHATLTVHVKPYFVKEPSDVTVIADQMVDFECRVGGDPSPKILWRRDDGKMPIGRAHLLDDKSLRIEHVTPEDEGLYICDAENLVGSVSARASLTVHSPPVFITKPQDQKVGLNGIASFDCVAKGNPPPSVFWSKEGSQVLMFPGNSYGHLHVTAEGSLRIQGAQREDAGFLVCSALSVAGSTTVRAFLQVTSVDDVPPPIVEIGPSNQTLPIDSVANLPCQARGTPHPIIKWYKDSQPLDSTVNPRVNILTSGTLQINDLQLVDSGLYTCTASSESGETSWSASLSVEKA-PGTHLHRSPDPSTFPSPPGTPQVLNITQSNITITWDP--GENAAPLIGYTV------------------------------EYFSSDLQTGWVVAAHRINNRSITIGDLKPETSYVFVVRAENAHGLSVPSDTSKVVRTLGSSEGTRTVPPQLLDEARVRLGTK-VIVLQDLSPVSSTSVKLVWEILNAEKFVEGLYVRFRDLS--GGSQKYNMVTVLNAGATSYTVVNLRKFTKYEFFLVPFFKTVEGQPSNSKIVQTLEDVPSAPPDNIQVGSINSTAVFVHWSPPPPQHYNGIILGYKLQIKSNGTKVVAQMSMNVSTTSILLNNLASNGMLTVRVAALTRVGIGPYS--VSMPLVFSRLGPYSQPTTGP------TQTWLVLILAFLAVILLLATVSTLYVRKRHGLTKHI 866 HSP 2 Score: 276.559 bits (706), Expect = 1.372e-73 Identity = 181/500 (36.20%), Postives = 254/500 (50.80%), Query Frame = 0
Query: 7 NVLFSFPGCWAASPPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSH--RVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE-------LDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQ---RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ 486
+V+ + G + + P ITEHP ++ V +NEPVTL C+A+G PEP I+W+KDG+ V +A + VLLP+GSLFFLRA KKE+D G+YWC A N G SRNA+L VAVL++ FR EP+D G TA+ ECGPPKG P PT FWKKDG++L+LE +D + +++ Q + L R E G +LT+ P P V+A DF+C G + R+ GK P R L + +L I V P+DEG Y C A+N+ G +SA+A L++ P + P++ F C KG P P + WS+ M P +H T L + ED C A + AG A LQV S PP+ + P++Q S +PC A G P PI+ W K+ L + V++ G L+I + DSG+YTC + SESG S+ + ++
Sbjct: 5 SVILNLSGQYRS--PRITEHPASTVVPKNEPVTLNCKAEGRPEPDIEWWKDGQRVEVSANTQRDSIGPVLLPAGSLFFLRARHGKKEHDDGVYWCVARNLAGSATSRNATLQVAVLREDFRAEPKDTRVAAGETALLECGPPKGHPEPTLFWKKDGQVLDLETSNRVRVVDGGNLMMTEVRQADEGRYQCLVQNLVGMR--ESGHATLTVHVKPYFVKEPSDVTVIADQMVDFECRVGGDPSPKILWRR--DDGKMPIGRAHL--LDDKSLRIEHVTPEDEGLYICDAENLVG-SVSARASLTVHSPPVFITKPQDQKVGLNGIASFDCVAKGNPPPSVFWSKEGSQVLMFPGNSYGHLHVTAEGSLRIQGAQREDAGFLVCSALSVAGSTTVRAFLQVTSVDDVPPPIVEIGPSNQTLPIDSVANLPCQARGTPHPIIKWYKDSQP---LDSTVNPRVNILTSGTLQINDLQLVDSGLYTCTASSESGETSWSASLSVE 492
BLAST of roundabout 1 vs. nr
Match: gi|815815103|ref|XP_012228596.1| (PREDICTED: roundabout homolog 2-like [Linepithema humile]) HSP 1 Score: 452.21 bits (1162), Expect = 1.901e-135 Identity = 293/901 (32.52%), Postives = 444/901 (49.28%), Query Frame = 0
Query: 127 EPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE---------LDP--SICWVQKIQGNSPSEEQKEKEKLFEARKD--EIGTESLTLSYCPKLD---TRPQIELVVACNREDFDC--PKGLEAIMGNFGR--QLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG-SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRY--LRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
P D+ + C +G P P W KDG +++ L P S+ +++ + G +EQ AR + + + TL+ D PQ V A +C P+G + R +I L EA +++ L+IS V+P D+G+YQC+A+N+ GI+ SA A L++ V P+ L P N + + T F C V G P PEI W R +G +P R D L + + PED+ Y C A+N G + A+A L V S P+F P D+ G+ V C A G P P +FW +EG ++ PG + QGH V+ G L I +KD G Y C+++S++GA + +++ + ++ PPPII++GP NQT + S PC A P RV W K+GV + RI + + TL ++L +DS LYTC A S SG SA+L V+ F P +S P +PS+P ++NATSN + + W P G S I Y IEYY+S T WV + +T+ D P T LVRA N HGLS P P+S QT +S +R +L + ++ LKEV+ L S VK+ W++L V G +I+ R++S+ Y ++T+ G S+ LT+L+ Y F++P+ I+GRP+ + +VTT EDVP+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +LT G Y +VA R G+GP P L ++ PQL P P G VV+E WF++++ +V ++ L + RR + K +GH N
Sbjct: 35 HPSDITVAKNEPVTLNC-KAEGKPEPVIEWYKDGELVQTSPGDAKSHRVLLPTGSLFFLRVMHGK---KEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLQWKRNGHIIDL----EATKRITLVDGSNLMISDVRPADQGKYQCIAENLVGIKESAIATLTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPPPEILWRRHDGKMPIGRGRILDDKSLRIERVNPEDQGTYICEADNGIGTISANATLTVHSKPIFTNFPKDETVNAGTNVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQGHYTVTEGGSLSIKGAVKKDEGYYVCSAISQAGASTVTVFLQVTSVEEIPPPIIELGPANQTLPLKSEAWLPCRAVGIPTPRVHWHKEGVLVQPGG--RITMAINGTLFIDDLRANDSGLYTCIASSESGNTSWSAYLTVSSG---ASFHRTPDISALPQNPSKPRIVNATSNSITLTW-SPGDEGQSKIIGYN------------------------------IEYYSSNLNTGWVVAATGVVDDIYTVPDLRPETNYVFLVRAENSHGLSLPGPLSDVVQTTNVNQHTVSQVELTRIRDRL-NSEILHLKEVQPLSSTSVKIMWDILGAADLVEGLYIRYREVSE---KPEYQMVTVLNAGATSYVLTNLKKFMRYEFFLVPFYKTIEGRPSNVKLVTTLEDVPSGAPENVHVGMINMTSAFVRWSPPPKNTQNGQLIGYKIQIKTNSSNKILGQMSLNASTTSVIINSLTTGGLYTARVAGQTRVGLGPFSSPTLLNMDPTQLTPQLPPRTDPSHGGASVVRETWFLVLMITMTVTVIAALLTALYVRRRQAMSKQLGHLN 887 HSP 2 Score: 306.605 bits (784), Expect = 1.858e-83 Identity = 195/497 (39.24%), Postives = 265/497 (53.32%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQ----KIQGNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVH----TTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLL-PGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT--SQDRPPPI 495
P ITEHP + +V +NEPVTL C+A+G PEP I+W+KDGE V T+ D KSHRVLLP+GSLFFLR + KKE DGG+YWC A N G V SRNA+L VAVL+D+FR EPQ+ G TA+ ECGPP+G P PT WK++G I++LE I V I P+++ K + E L ++ I T LT+ P P + ++ +F C G + R+ GK P R I + +L I +V P+D+G Y C A N G +SA A L++ P P++ + AG V F CA +G P P I W+R M P T+G L++ V +DE Y C A ++AG + LQV S PP+ + P +Q S +PC A G P P + W KE GVL+ PG G + ++ G L I + DSG+YTC + SESG S+ + + + S R P I
Sbjct: 30 PRITEHPSDITVAKNEPVTLNCKAEGKPEPVIEWYKDGELVQTSPGDAKSHRVLLPTGSLFFLRVMHGKKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLQWKRNGHIIDLEATKRITLVDGSNLMISDVRPADQGKYQCIAENLVGIKESAIAT--LTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPPPEILWRR--HDGKMPIGRG--RILDDKSLRIERVNPEDQGTYICEADNGIGT-ISANATLTVHSKPIFTNFPKDETVNAGTNVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQGHYTVTEGGSLSIKGAVKKDEGYYVCSAISQAGASTVTVFLQVTSVEEIPPPIIELGPANQTLPLKSEAWLPCRAVGIPTPRVHWHKE---GVLVQPG---GRITMAINGTLFIDDLRANDSGLYTCIASSESGNTSWSAYLTVSSGASFHRTPDI 513
BLAST of roundabout 1 vs. nr
Match: gi|826448266|ref|XP_012531725.1| (PREDICTED: roundabout homolog 2-like [Monomorium pharaonis]) HSP 1 Score: 451.055 bits (1159), Expect = 5.331e-135 Identity = 293/900 (32.56%), Postives = 447/900 (49.67%), Query Frame = 0
Query: 127 EPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE---------LDP--SICWVQKIQGNSPSEEQKEKEKLFEARKD--EIGTESLTLSYCPKLD---TRPQIELVVACNREDFDC--PKGLEAIMGNFGR--QLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG-SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS-DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
P D+ + C +G P P W KDG +++ L P S+ +++ + G +EQ AR + + + TL+ D PQ V A +C P+G ++ R +I L EA +++ G L+IS V+P D+G+YQC+A+N+ G + SA A L++ V P+ L P N + + T F C V G P PEI W R +G +P R H + L + + P+D+ Y C AEN G + ASA L V S PVF P D+ G+ V C A G P P +FW +EG ++ PG + Q H +V+ +G L I KD G Y C+++S++GA A+ +++ + ++ PPPII++GP NQT + S + PC A TP RV+W K+GV + + RI + + TL ++L +DS LYTC A S SG SA+L V+ F P +S P +PS+P ++NATSN + + W P G S I Y IEYY+S T WV + +T+ D P T LVRA N GLS P P+S QT + R++ + ++ LKEV+ L S VK+ W++L V G +I+ R+ S+ Y ++T+ G S+ LT+L+ T Y F++P+ I+GRP+ + +VTT EDVP+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +LT G Y +VA R G+GP +P L ++ QL P P G VV+E WF++++ ++ LA + RR + K +GH N
Sbjct: 33 HPSDIIVAKNEPVTLNC-KAEGKPEPVIEWYKDGELVQTSPGDAKSHRVLLPTGSLFFLRVMHG---KKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDL----EATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAIATLTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPEPEILWRRSDGKMPIGRAHILNDKSLHIERVNPQDQGTYICDAENSVGAISASATLTVHSRPVFTNFPKDETVNAGADVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQTHYNVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSIEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVQWHKEGVLVQSGG--RITMAINGTLFIDDLHAADSGLYTCIASSESGNTSWSAYLTVS--STGASFHRTPDVSALPQNPSKPRIVNATSNSVTLTW-SPGHEGQSKIIGYN------------------------------IEYYSSNLNTGWVVAATGVLDDIYTVTDLRPETNYVFLVRAENSQGLSLPGPLSDLVQTTNVNQHTVPQVELTRARDRLNSEILHLKEVQPLTSTSVKIMWDILGAADLVEGLYIRYREDSE---KPEYQMVTVLNAGATSYVLTNLKKYTRYEFFLVPFYKTIEGRPSNVKLVTTLEDVPSGAPENVHVGMINMTSAFVRWSPPPKNTQNGQLIGYKIQIKSNSSNKILGQMSLNASTTSVIINSLTTGGLYTARVAGQTRVGLGPFSNPTLLNMDPGQL-TQLPPRTDPSHGGASVVRETWFLVLIITMIFTVIAALLAALYVRRRQAMSKQLGHLN 885 HSP 2 Score: 306.99 bits (785), Expect = 1.080e-83 Identity = 189/487 (38.81%), Postives = 262/487 (53.80%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQ----KIQGNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHT----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTS 488
P ITEHP + V +NEPVTL C+A+G PEP I+W+KDGE V T+ D KSHRVLLP+GSLFFLR + KKE DGG+YWC A N G V SRNA+L VAVL+D+FR EPQ+ G TA+ ECGPP+G P PT WK++G I++LE I V I P+++ K + E + ++ I T LT+ P P + ++ +F C G + R+ GK P R I + +L I +V P+D+G Y C A+N G +SA A L++ P P++ + AG V F CA +G P P I W+R M P T T+ L++ V +DE Y C A ++AG A+ LQV S PP+ + P +Q S +PC A G P P + W KE GVL+ + G + ++ G L I + DSG+YTC + SESG S+ + + ++
Sbjct: 28 PRITEHPSDIIVAKNEPVTLNCKAEGKPEPVIEWYKDGELVQTSPGDAKSHRVLLPTGSLFFLRVMHGKKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDLEATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAIAT--LTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPEPEILWRR--SDGKMPIGR--AHILNDKSLHIERVNPQDQGTYICDAENSVGA-ISASATLTVHSRPVFTNFPKDETVNAGADVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQTHYNVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSIEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVQWHKE---GVLV--QSGGRITMAINGTLFIDDLHAADSGLYTCIASSESGNTSWSAYLTVSST 502
BLAST of roundabout 1 vs. nr
Match: gi|795043141|ref|XP_011867498.1| (PREDICTED: roundabout homolog 2-like [Vollenhovia emeryi]) HSP 1 Score: 450.284 bits (1157), Expect = 1.226e-134 Identity = 262/752 (34.84%), Postives = 394/752 (52.39%), Query Frame = 0
Query: 255 EARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG-SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS-DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
EA +++ G L+IS V+P D+G+YQC+A+N+ G + SA A L++ V P+ L P N + + T F C V G P PEI W R +G +P R H + L + + P+D+ Y C AEN G + ASA L V S P+F P D+ G+ V C A G P P +FW +EG ++ PG + Q H V+ +G L I KD G Y C+++S++GA A+ +++ + ++ PPPII++GP NQT + S + PC A TP RV W K+GV + RI + + TL ++L +DS LYTC A S SG SA+L V+ F P +S P +PS+P ++NATSN + + W P G S I Y IEYY+S T WV + +T+ D P T LVRA N GLS P P+S QT + R++ + ++ LKEV+ L S VK+ W++L V G +I+ R++S+ Y ++T+ G S+ LT+L+ T Y F++P+ I+GRP+ + +VTT EDVP+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +LT G Y +VA R G+GP P L ++ QL P P G VV+E WF++++ + ++V LA + RR + K +GH N
Sbjct: 173 EATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAIAVLTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPEPEILWRRNDGKMPIGRAHILNDKSLHIERVNPQDQGTYICDAENGVGAISASATLTVHSRPIFTNFPKDETVNAGTDVTFSCAARGAPNPSIFWTREGSQELMFPGHTYQSHYTVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGG--RITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTVSTG---ASFHRTPDVSALPQNPSKPRIVNATSNSVTLTW-SPGHEGQSKIVGYN------------------------------IEYYSSNLNTGWVVAATGVVDDIYTVTDLRPETNYVFLVRAENSQGLSLPGPLSDVVQTTNVNQHTVPQVELTRARDRLNSEILHLKEVQPLTSTSVKIMWDILGAADLVEGLYIRYREVSE---KPEYQMVTVLNAGATSYVLTNLKKYTRYEFFLVPFYKIIEGRPSNVKLVTTLEDVPSGAPENVHVGMINMTSAFVRWSPPPKNTQNGQLVGYKIQIKSNSSNKILGQMSLNASTTSVIINSLTTGGLYTARVAGQTRVGLGPFSSPTLLNMDPGQL-TQLPPRTDPSHGGASVVRETWFLVLIITMILTVIVALLAALYVRRRQAMSKQLGHLN 884 HSP 2 Score: 307.375 bits (786), Expect = 9.479e-84 Identity = 190/487 (39.01%), Postives = 262/487 (53.80%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQK----IQGNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHT----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLL-PGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT 487
P ITEHP + V +NEPVTL C+A+G PEP I+W+KDGE V T+ D KSHRVLLP+GSLFFLR + KKE DGG+YWC A N G V SRNA+L VAVL+D+FR EPQ+ G TA+ ECGPP+G P PT WK++G I++LE I V I P+++ K + E + ++ I LT+ P P + ++ +F C G + R+ GK P R I + +L I +V P+D+G Y C A+N G +SA A L++ P P++ + AG V F CA +G P+P I W+R M P + T+ L++ V +DE Y C A ++AG A+ LQV S PP+ + P +Q S +PC A G P P + W KE GVL+ PG G + ++ G L I + DSG+YTC + SESG S+ + + T
Sbjct: 28 PRITEHPSDIIVAKNEPVTLNCKAEGKPEPLIEWYKDGELVQTSPGDAKSHRVLLPTGSLFFLRVMHGKKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDLEATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAIAV--LTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPEPEILWRR--NDGKMPIGR--AHILNDKSLHIERVNPQDQGTYICDAENGVGA-ISASATLTVHSRPIFTNFPKDETVNAGTDVTFSCAARGAPNPSIFWTREGSQELMFPGHTYQSHYTVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKE---GVLVRPG---GRITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTVST 501
BLAST of roundabout 1 vs. nr
Match: gi|1070595524|ref|XP_018394917.1| (PREDICTED: roundabout homolog 2-like [Cyphomyrmex costatus]) HSP 1 Score: 449.899 bits (1156), Expect = 1.479e-134 Identity = 293/900 (32.56%), Postives = 445/900 (49.44%), Query Frame = 0
Query: 127 EPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE---------LDP--SICWVQKIQGNSPSEEQKEKEKLFEARKD--EIGTESLTLSYCPKLD---TRPQIELVVACNREDFDC--PKGLEAIMGNFGR--QLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG-SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS-DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
P D+ + C +G P P W KDG +++ L P S+ +++ + G +EQ AR + + + TL+ D PQ V A +C P+G ++ R +I L EA +++ G L+IS V+P D+G+YQC+A+N+ G + SA A L++ V PY L P N + +A T F C V G P PEI W R +G +P R H + L + + P+D+ Y C AEN G + ASA L V S PVF P D+ G+ V C A G P P +FW +EG ++ PG + Q H V+ +G L I KD G Y C+++S++GA A+ +++ + ++ PPPII++GP NQT + S + PC A TP RV W K+GV + RI + + TL ++L +DS LYTC A S SG SA+L ++ F P +S P +PS+P ++NATSN + + W P G S I Y IEYY+S T WV + +T+ D P T LVRA N GLS P P+S QT + R++ + ++ LKEV+ L S VK+ W++L V G +I+ R++S+ Y ++T+ G S+ LT+L+ T Y F++P+ I+GRP+ + +VTT ED+P+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +LT G Y +VA R G+GP P L ++ QL P P G VV+E WF++++ ++ LA + RR + K +GH N
Sbjct: 33 HPSDIIVAKNEPVTLNC-KAEGKPEPVIEWYKDGELVQTSPGDTKSHRVLLPTGSLFFLRVMHG---KKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDL----EATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAVAVLTVHVKPYFLAMPSNQTILADQTAEFACRVGGDPEPEILWRRNDGKMPIGRAHILNDKSLHIERVNPQDQGTYICDAENGVGAISASATLTVHSRPVFTNFPKDETVNAGTDVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQSHYTVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGG--RITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTISTG---ASFHRTPDMSALPQNPSKPRIVNATSNSVTLTW-SPGHEGQSKIIGYN------------------------------IEYYSSNLNTGWVVAATGVLDDIYTVTDLRPETNYVFLVRAENSQGLSLPGPLSDVVQTTNANQHTVPQVELTRARDRLNSEILHLKEVQPLTSTSVKIMWDILGAADLVEGLYIRYREVSE---KPEYQMVTVLNAGATSYVLTNLKKYTRYEFFLVPFYKIIEGRPSNVKLVTTLEDMPSGAPENVHVGMINMTSAFVRWSPPPKNTQNGQLIGYKIQIKSNSSNKILGQMSLNASTTSVIINSLTTGGLYTARVAGQTRVGLGPFSSPTLLNMDPGQL-TQLPPRTDPSHGGASVVRETWFLVLIITMIFTVIAVLLAALYVRRRQAMSKQLGHLN 884 HSP 2 Score: 309.301 bits (791), Expect = 2.062e-84 Identity = 189/486 (38.89%), Postives = 261/486 (53.70%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQ----KIQGNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHT----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT 487
P ITEHP + V +NEPVTL C+A+G PEP I+W+KDGE V T+ D KSHRVLLP+GSLFFLR + KKE DGG+YWC A N G V SRNA+L VAVL+D+FR EPQ+ G TA+ ECGPP+G P PT WK++G I++LE I V I P+++ K + E + ++ + LT+ P P + ++A +F C G + R+ GK P R I + +L I +V P+D+G Y C A+N G +SA A L++ P P++ + AG V F CA +G P P I W+R M P + T+ L++ V +DE Y C A ++AG A+ LQV S PP+ + P +Q S +PC A G P P + W KEG ++ PG G + ++ G L I + DSG+YTC + SESG S+ + I T
Sbjct: 28 PRITEHPSDIIVAKNEPVTLNCKAEGKPEPVIEWYKDGELVQTSPGDTKSHRVLLPTGSLFFLRVMHGKKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDLEATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAVAV--LTVHVKPYFLAMPSNQTILADQTAEFACRVGGDPEPEILWRR--NDGKMPIGR--AHILNDKSLHIERVNPQDQGTYICDAENGVGA-ISASATLTVHSRPVFTNFPKDETVNAGTDVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQSHYTVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEG--VLVRPG---GRITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTIST 501
BLAST of roundabout 1 vs. nr
Match: gi|307209011|gb|EFN86211.1| (Roundabout-like protein 2 [Harpegnathos saltator]) HSP 1 Score: 447.588 bits (1150), Expect = 1.869e-134 Identity = 266/754 (35.28%), Postives = 391/754 (51.86%), Query Frame = 0
Query: 255 EARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSN-QGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS-DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWF--VMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
EA +++ G L+IS V+ D+G+YQC+A+N+ G + SA A L++ V P+ L P N + + T F C V G P PEI W R +G +P R H D L + + P+D Y C AEN G + ASA L V S PVF P D+ G+ V I C A G P P +FW +EG ++ PG+ Q H V+ +G L I T KD G Y C+++S++GA A+ +++ + ++ PPPII++GP NQT + S + PC A TP RV W K+GV + RI + + TL ++L +DS LYTC A S SG SA+L V+ F P +S P +PS+P ++NATSN + + W P G S I Y V EYY+S T WV + +T+ D P T LVRA N HGLS P P+S QT + R++ + ++ LKEV+ L S VK+ W++L V G +I+ R++S+ Y ++T+ G S+ L LR + Y F++P+ I+GRP+ + + TT EDVP+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +L G Y +VA R G+GP P L ++ QL P P G VV+E WF +M+ F+VI + LA + RR + K +GH N
Sbjct: 78 EATKRITLVDGGNLMISDVRQTDQGKYQCIAENMVGAKESAIATLTVHVKPFFLTTPSNQTILTDQTAEFACRVGGDPPPEILWRRSDGKMPIGRAHILDDKSLRIERVNPQDHGTYICQAENGVGTISASATLTVHSKPVFTNFPKDETVSAGTDVTISCAARGAPKPSIFWTREGSQELMFPGNTYQSHYTVTEDGSLSIKATVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGG--RITMAINGTLFIDDLRSNDSGLYTCIASSESGNTSWSAYLTVSAG---ASFHRTPDISALPQNPSKPRIVNATSNSVTLTW-SPGHEGQSKILGYNV------------------------------EYYSSNLNTGWVVAATGVLDDIYTVTDLRPETNYVFLVRAENSHGLSLPGPLSDVVQTTNVNQHTVPQVELTRARDRLNSEILHLKEVQALSSTSVKIMWDILGAADLVEGLYIRYREVSE---KPEYQMVTVLNAGATSYVLASLRKYSRYEFFLVPFYKIIEGRPSNVKLATTMEDVPSGAPENVHVGMINMTSAYVRWSPPPKNTQNGQLIGYKIQIKSNSSNKILGQMSLNASTTSVIINSLNTGGLYTARVAGLTRIGLGPFSSPTLLNMDPGQL-TQLPPRTDPSHGDASVVRETWFLVLMITMVFTVIAV--LLAALYVRRRQAMSKQLGHLN 789 HSP 2 Score: 182.185 bits (461), Expect = 6.287e-43 Identity = 129/391 (32.99%), Postives = 189/391 (48.34%), Query Frame = 0
Query: 105 FGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE-------LDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHT----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLL-PGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESG 475
+++ R A L VAVL+D+FR EPQ+ G TA+ ECGPP+G P PT WK++G I++LE +D + ++ + Q E + A++ I T LT+ P T P + ++ +F C G + R+ GK P R I + +L I +V P+D G Y C A+N G +SA A L++ P P++ + AG V CA +G P P I W+R M P + T+ L++ V +DE Y C A ++AG A+ LQV S PP+ + P +Q S +PC A G P P + W KE GVL+ PG G + ++ G L I + DSG+YTC + SESG
Sbjct: 17 LSQIQWRIAGLSVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDLEATKRITLVDGGNLMISDVRQTDQGKYQCIAENMVGAKESAIAT--LTVHVKPFFLTTPSNQTILTDQTAEFACRVGGDPPPEILWRR--SDGKMPIGR--AHILDDKSLRIERVNPQDHGTYICQAENGVGT-ISASATLTVHSKPVFTNFPKDETVSAGTDVTISCAARGAPKPSIFWTREGSQELMFPGNTYQSHYTVTEDGSLSIKATVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKE---GVLVRPG---GRITMAINGTLFIDDLRSNDSGLYTCIASSESG 394 HSP 3 Score: 86.6557 bits (213), Expect = 1.890e-13 Identity = 100/396 (25.25%), Postives = 160/396 (40.40%), Query Frame = 0
Query: 27 PQNSSVTRNEPVTLRCEA-QGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKK-DGRI---LELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGT----ESLTLSYCPKLDTRPQIELVVACNREDFDC-----PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVL-------PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGML-PAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKP 400
PQN+ V E L C +G PEP + W ++G + A + R+ L G + V ++ D G Y C A N G S A+L V V K F P + + TA F C G P P W++ DG++ LD ++++ Q + +A ++ +GT +LT+ P P+ E V A C PK ++L+ G ++ + ++ +G+L I KDEG Y C A + AG ++ A + LQV P + P N + C G P P + W + ++ P R+ L + ++ D +YTC+A + +G SA+L V + F P
Sbjct: 40 PQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDLEA----TKRITLVDGGNLMISDV---RQTDQGKYQCIAENMVGAKESAIATLTVHV-KPFFLTTPSNQTILTDQTAEFAC-RVGGDPPPEILWRRSDGKMPIGRAHILDDKSLRIERVN------PQDHGTYICQA-ENGVGTISASATLTVHSKPVFTNFPKDETVSAGTDVTISCAARGAPKPSIFWTREGSQELMFPGNTYQSHY--TVTEDGSLSIKATVRKDEGHYVCSAISQAG---ASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGGRITMAINGTLFIDDLRSNDSGLYTCIASSESGNTSWSAYLTVSAGASFHRTP 414 The following BLAST results are available for this feature:
BLAST of roundabout 1 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 25
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BLAST of roundabout 1 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of roundabout 1 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold49_size462716:377790..401551+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold49_size462716-snap-gene-3.30 ID=maker-scaffold49_size462716-snap-gene-3.30|Name=roundabout 1|organism=Tigriopus kingsejongensis|type=gene|length=23762bp|location=Sequence derived from alignment at scaffold49_size462716:377790..401551+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'roundabout 1' has the following synonyms
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