roundabout 1, maker-scaffold49_size462716-snap-gene-3.30 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000001266 (supercontig:LSalAtl2s:LSalAtl2s1208:7071:89755:-1 gene:EMLSAG00000001266 transcript:EMLSAT00000001266 description:"maker-LSalAtl2s1208-augustus-gene-0.12") HSP 1 Score: 97.4413 bits (241), Expect = 9.788e-21 Identity = 151/629 (24.01%), Postives = 230/629 (36.57%), Query Frame = 0
Query: 1 MRANPLNVLFSFPGCWAASPPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKD------KFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSF---W------KKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKD-EIGTESLTLSYC---PKLDTRPQIEL----------VVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGE--GTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQ-RVHTTDGTQLTLMNIVPE-DEDVYTCLAENRAGF-VEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPI-LFWMKEGGSGVLLPGSNQGHVHVSPEG---MLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC---EATPQTRVEWLKDGVPI-----STMNDPRIKV--DHDNTLHFEELEIS-DSALYTCQAKSTSGQAMLSAFLRV 580
MR+ P N+L S G A S + + + L C + G P P WFK+ ++ + + P GS + +QS +D GIY C A N G + S+ + V R+ P A G A F C P + W +D RIL + I VQ+ G K ++A + +G L Y L P + L ++ + FD P ++G + +++ G+ L IS+ D G Y C N AG + + RL++ P + PE + A+ G T C + G P + W + N +L + R + L L ++ E DE Y C NR G + V P + QG + + C A P+ L W+K +N G +S + ML I S G YTC + +++G+ S + + PP I+ R GS T C R+ WLKDG + S +D +I D + L L S +YTC A++ +GQA A L V
Sbjct: 1 MRSKP-NILHSKLGASAPSEREVLY------MEEGQGAILPCFSSGSPPPFTSWFKENHHLLDPLPSSPQYHLFGPRGSALKIYRLQS---SDSGIYRCVARNDVGE---SSFSIHLKVYSQGESSPPDLRVLPDIQSADIGSNARFSCQIPDSRISQIEVGLRWLVNGVSPRDPRILVRGDEIEIRNVQQEDGGIYQCFLSLKGNEYQASGELRLGDSLPRLQYAFIEQTLQPGPMVSLKCSATGSPTPIIKWKLDGFDLPNLERYLIGQY----------------VTVHGDVISHLNISRSLVIDGGLYSCSVINRAG-SIIHQGRLNIYGAPMIRPWPEEIKAIEGRTFVLHCPLGGFPIEAVHWEKDNLLLASNLRRKVLNNGTLILSDVAKERDEGSYKCTGSNRQGLSAMGKTKISVIVSPKIVPFAAPASLQQGERISLTCTIARGDAPLTLSWVKNNRP----LNNNVGQKIISFDSYNSMLTIEALSLHHIGTYTCLARNKAGSAEYSQHITVHV-----PPEIEPFTFGDNLREGSRTRVVCGILRGDLPIRLSWLKDGSHLLNGQSSGESDLQIASVDDFSSLLTISNLRYRLHSGVYTCVAENPAGQATFDAPLTV 590 HSP 2 Score: 87.0409 bits (214), Expect = 1.507e-17 Identity = 108/433 (24.94%), Postives = 165/433 (38.11%), Query Frame = 0
Query: 38 VTLRCEAQGDPEPQIKWFKDGEPV-----------VTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDE----IGTESLTLSYCPK---------LDTRPQIELVVACNRED----FDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAV-KGRPHPEIRWSRRNG-MLPAQRVHTTD---------GTQLTLMNIVPE-DEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKE 430
V+L+C A G P P IKW DG + VT D SH L S SL DGG+Y C N G + + + R P+++ AI+G T + C P G P W+KD +L L + + + ++E+ E + +G +++ PK L +I L R D K + N G+++I +L+IE L + + G Y C+A+N AG +++ V P + + G R C + +G + W + +L Q +D + LT+ N+ VYTC+AEN AG A L V PP + KP VQG + ++ C A G+P P + W KE
Sbjct: 225 VSLKCSATGSPTPIIKWKLDGFDLPNLERYLIGQYVTVHGDVISH--LNISRSLVI----------DGGLYSCSVINRAGSIIHQGRLNIYGA--PMIRPWPEEIKAIEGRTFVLHC-PLGGFPIEAVHWEKDNLLLASNLRRKVLNNGTLILSDVAKERDEGSYKCTGSNRQGLSAMGKTKISVIVSPKIVPFAAPASLQQGERISLTCTIARGDAPLTLSWVKNNRPLNNNVGQKIISFDS---YNSMLTIEA---LSLHHI-----GTYTCLARNKAG-SAEYSQHITVHVPPEIEPFTFGDNLREGSRTRVVCGILRGDLPIRLSWLKDGSHLLNGQSSGESDLQIASVDDFSSLLTISNLRYRLHSGVYTCVAENPAGQATFDAPLTVKVPPAWKTKPWSTSSVQGESSKLYCQAEGSPAPKISWKKE 630 HSP 3 Score: 72.4034 bits (176), Expect = 5.350e-13 Identity = 51/191 (26.70%), Postives = 87/191 (45.55%), Query Frame = 0
Query: 407 QGSTVQIPCVAAGNPVPILFWMKEGGSGVL-LPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQTRV-------EWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEF 589
+G +PC ++G+P P W KE + LP S Q H+ L+I DSG+Y C + ++ G S +K+ + + PP +++ P Q+ IGS+ F C+ P +R+ WL +GV + DPRI V D + ++ D +Y C + S LR+ + +++
Sbjct: 27 EGQGAILPCFSSGSPPPFTSWFKENHHLLDPLPSSPQYHLFGPRGSALKIYRLQSSDSGIYRCVARNDVGESSFSIHLKVYSQGESSPPDLRVLPDIQSADIGSNARFSCQ-IPDSRISQIEVGLRWLVNGV---SPRDPRILVRGDE-IEIRNVQQEDGGIYQCFLSLKGNEYQASGELRLGDSLPRLQY 212
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000006069 (supercontig:LSalAtl2s:LSalAtl2s329:314766:492433:1 gene:EMLSAG00000006069 transcript:EMLSAT00000006069 description:"maker-LSalAtl2s329-augustus-gene-5.12") HSP 1 Score: 96.2857 bits (238), Expect = 1.252e-20 Identity = 56/178 (31.46%), Postives = 89/178 (50.00%), Query Frame = 0
Query: 314 VSAVAGDTVRFGCAVKGRPHPEIRWSR-RNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKP--TDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTS 488
++ ++G+ + C G P P+I W++ + T G L L NI P E +Y C A + G ASA+++ PP+ V T GS +++ C A G P P+L WM+E +LLPG + VS EG L + T +D+ Y C V+ +GA +A + V +++S
Sbjct: 1 MTVISGNDIILECVGDGDPSPKIIWTKGERSDIDTNYEQTIPGQGLKLRNIHPAQEGMYKCTATSPIGSTSASAYVRXIEPPLITVHSPRTVTAPALGSKIELDCSAEGRPEPVLLWMREDDRTILLPGDRXDTLEVSAEGTLIVKAT--QDTTHYICLVVNNAGAAVARTTVHLRSS 176 HSP 2 Score: 57.3806 bits (137), Expect = 1.521e-8 Identity = 45/137 (32.85%), Postives = 65/137 (47.45%), Query Frame = 0
Query: 261 SIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKH-PENVSAVA-GDTVRFGCAVKGRPHPEIRWSR---RNGMLPAQRVHTTD-GTQLTLMNIVPEDEDVYTCLAENRAGFVEA--SAHLQ 389
+I G+G L + + P EG Y+C A + G SA A + P + H P V+A A G + C+ +GRP P + W R R +LP R T + + TL+ +D Y CL N AG A + HL+
Sbjct: 40 TIPGQG-LKLRNIHPAQEGMYKCTATSPIG-STSASAYVRXIEPPLITVHSPRTVTAPALGSKIELDCSAEGRPEPVLLWMREDDRTILLPGDRXDTLEVSAEGTLIVKATQDTTHYICLVVNNAGAAVARTTVHLR 174
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000012875 (supercontig:LSalAtl2s:LSalAtl2s97:3013984:3060502:1 gene:EMLSAG00000012875 transcript:EMLSAT00000012875 description:"maker-LSalAtl2s97-augustus-gene-29.23") HSP 1 Score: 95.9005 bits (237), Expect = 1.905e-20 Identity = 69/238 (28.99%), Postives = 111/238 (46.64%), Query Frame = 0
Query: 760 VKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEALNFTLEPSSNSLILYNLTEGLTY---AVQVAAYNRQGVGPLCDPVEL------EVETDLFHPQLVPEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIR 988
+KL W+L + G + R + + S+ I S+ L DL+ TEY +F+ PY + GRP++LV + T VP AP+ +KI++LN TA I W R +G++KG+++ I N +E LN L PS + N+ + LT V +A N GVGP D E+ E + ++H + E W + ++ F+ +L + +RR R
Sbjct: 155 IKLEWKLASTISSINGIRLHYRK----NGNKSFVSKVIPLDRDTSYLLQDLQEFTEYEIFIRPYYESVSGRPSRLVQIITLPTVPDQAPKILKIKILNATAIFIAWDYVDREHHNGNIKGYQIHIQGNNSEILNSAL-PSERLESILNVPKLLTIDKANVSIALINHVGVGPFSDSEEILLPSLSEFDMAVYHHRGSGESY---------AWLLALIGSFTFMLAFASGLTLYYRRRR 378
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000007100 (supercontig:LSalAtl2s:LSalAtl2s3:931395:944048:1 gene:EMLSAG00000007100 transcript:EMLSAT00000007100 description:"maker-LSalAtl2s3-augustus-gene-9.8") HSP 1 Score: 96.6709 bits (239), Expect = 2.482e-20 Identity = 143/615 (23.25%), Postives = 244/615 (39.67%), Query Frame = 0
Query: 20 PPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLP-------SGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVA------VLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEAR------KDEIG-TESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKR-----PEARFLLSIEGE-GT--LIISQVQPKDEGQYQCVAKNVAGI-----RLSAKARLSLQV---LPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHT-TDG--TQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQV---FSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLR---IGNTSEKDSGVYTCASVSESGAILASSEVKI-----QTSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVDHDNT----LHFEELEISDSALYTCQAKSTSGQAMLSAFL 578
P + + P+ V + L + G P P W +GE + AD RV++ SG + LR + S K+ D G Y NT G + +V V+K+ IEP+++ I G + G P PT W +DG E+++ + Q G + EK ++ +A +E G E+ + P+ ++ + P LE + + I+ K P+ + S + E GT LI D+G Y VA + G+ ++ K+R+ V P ++ +V+ G +R C VKG P PE W++ + ++R H +DG + ++N ED Y C N G + +++V + PP F + K + G + C NP P + W G P + G + G +R + ++ D G Y C + ++ G +S ++I + D P + +IG ++ G S F TP+ E+ +G P+ + RI + + T L +E SD +Y + +T G+A A L
Sbjct: 985 PTIEADFPRMIEVKEGKEFVLTAKIDGSPPPTAIWLCEGEEI---KAD--GERVIITEEEAEDGSGIITTLR-ITSAKDEDNGKYTLLVKNTAGEEKMDTMVDIVGKPKPPKVVKE---IEPKEI-TIPGKKELRLTCKISGYPMPTIKWYRDGN--EIKVRKGVLISQDASGGATL--VLEKCQMXDAGTXSAKGXNEXGDAETFANVTVTQPMEEPKFTSLLRSAKAVEGSPIKLEGKVSGHPQPEIKWLKNEVEFIPDGDRVQSFQNEDGTFGLIFECTNADDKGTYTAVAVSPEGMARSNGNVTIKSRMKEGVDKSKPSFVRPMGDVAVDEGQKLRITCPVKGNPIPEFSWTKDGKPIASERAHCFSDGELIGIEVINSKMEDAGAYECHLSNELGTITGVCNVKVNKIYQPPFFSTPLNNVKQIVGCDARFVCDVGCNPKPDITWTFNGK-----PIDDGGKYTIKTNGNMRTLCVKKINDADIGTYKCIATNKEGTKDSSGTLEIVEFIEKGKSDAPEFLKKIG--DELVFRGMSARFTALVTGTPEPDYEFFFNGKPLYPTD--RIHIVRERTGLIRLSMAYVEESDIGVYGLKVTNTHGEAYCEARL 1576 HSP 2 Score: 93.9745 bits (232), Expect = 1.457e-19 Identity = 136/623 (21.83%), Postives = 242/623 (38.84%), Query Frame = 0
Query: 32 VTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRI-----EPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSP---------SEEQKEKEKLFEARKDEIGTE----SLTLSYCPKLDTRPQIEL-----------VVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGT-----LIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSR--------RNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGV-------QGSTVQIPCVAAGNPVPILFWMKEG------GSGVLL--PGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPII-QIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDN--TLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSV 592
V + T E G+P+P KW K+G+ + D K R ++ + ++ ++ +D G Y E TN G + ++ +V + D+ +I + + A +GG F+ +G P P W + ++E+ + ++ N SEE K ++ K+E G + SL + + P++ + + + A + D K + G I G + RF + +GT LII V +D G+Y+C+ N G S + P K E+++ G T F C V G P PEI W R + + + ++T +IV +D A+N G E++A Q+ +KPT + +G + G+P P W+ EG G V++ + G ++ LRI + ++D+G YT + +G + V I + +PP ++ +I P T C+ + P ++W +DG I I D TL E+ ++ D+ + + + G A A + V E +F S+
Sbjct: 594 VFEKDDFTFAVECDGNPKPVCKWTKEGQSI-----DTKDGRFVITENNGVYMLGIKEVTXDDKGXYXXEFTNRAGDRKVQSELIVHSA--DELKIPKCMSDLKSKKAEKGGKTFFQI-KIRGDPIPEVKWYFN----DIEVVNDDHFQVSVKENEHMYRLDVVGISEEHYGKVRV--VAKNENGEDFKEASLEVQFKPEIQAPEEFKAGPGDEASLSFKIRAFPGAEVDFYKKIAQEEGEPKMDKIEKGNKEYTRFSTEMTEDGTFEVHSLIIKDVVMEDSGEYECLXSNRIGNTSSLVKFAVVTEEPSFPKSLEDITTTLGSTETFECIVAGIPRPEIAWFRGDKELKKGKRXLFEEELAENGIKYKMTTRDIVFKDFGQIKLTAKNMVGESESTAIFQIVQ-----IKPTIEADFPRMIEVKEGKEFVLTAKIDGSPPPTAIWLCEGEEIKADGERVIITEEEAEDGSGIIT---TLRITSAKDEDNGKYTLLVKNTAGEEKMDTMVDI-VGKPKPPKVVKEIEPKEITIPGKKELRLTCKISGYPMPTIKWYRDGNEIKVRKGVLISQDASGGATLVLEKCQMXDAGTXSAKGXNEXGDAETFANVTVTQPMEEPKFTSL 1193 HSP 3 Score: 78.9518 bits (193), Expect = 5.662e-15 Identity = 122/529 (23.06%), Postives = 195/529 (36.86%), Query Frame = 0
Query: 54 WFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDK------------FRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSP----SEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNF--GRQLIRLGKRPEARFLLSIEGEG---TLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQV-LPYVLKHPE---------NVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHT-----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGM-LRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQTRVEWLKDGVPISTMNDPRIKVDHD 545
W KD +P+ + AD R L + F + + + D G Y C T G + +A L V L F ++ +D I TA F KG+P P + KDG ++ + +I G+ + ++ + + +GTE S K D + L+ ++ G E F G++ PE RF + + E L+ + P+D G Y CVA G ++S A LS++ + +LK PE + + G + ++G P P I+W++ + A + L + + PED Y N G + L + P F DQK +I +GNP L W K G L S + V PE L T D+G + + + G + E Q +I + N+ ++ C +P V+W K G I+ DPR+KV D
Sbjct: 37 WLKDNKPLDASVAD----RTKLLAKENHFSLDLSNVQPGDSGQYSCRVTGEGGTAITCSAVLEVHTLSPAEKREREESNHPVFLVKLRDADVILSSTASFMIHV-KGNPNPDVKFLKDGTEIKEDSHFTINRDHAPHGSYEVIIHDVKAEDAGQYSVVASNTLGTEQCAASISVK-DAKDVFSLLRGHEKK---VAPGEEPTFTWFKSGQEF-----DPEDRFKVLFKDEEDTLALVFQHINPEDAGXYTCVASTSTG-KISCSAELSVEGGVSQLLKEPEKPKILTGLGDADSSVGGSAMLELKMRGYPRPSIKWTKDGQPITASDHFKFVYPDQESVALIINKVQPEDAGAYKVTLTNDLGEASSEGKLTLSGAPQFQDTIEDQKTAVDDAWKIVTKVSGNPE--LTWYKNGVPITL--DSRIKSIKVDPETFELVFAKTLADDNGNWAVIARNAHGEMSQFFEFSAQMLPKFETRLIDV-EANEGKQAILKCKINC--SPDPEVKWFKGGTEIT--KDPRVKVYKD 541 HSP 4 Score: 70.8626 bits (172), Expect = 1.551e-12 Identity = 74/333 (22.22%), Postives = 135/333 (40.54%), Query Frame = 0
Query: 268 LIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG--MLPAQRVHTT----DGTQLTLMNIVPEDEDVYTCLAENRAG----FVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGM--LRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHF---EELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEE 585
LII++VQP+D G Y+ N G S++ +L+L P E+ D + V G +PE+ W +NG + R+ + + +L + +D + +A N G F E SA + P F + D + +G + C +P P + W K G + + V+ P G+ L I + S +G Y ++++ G +K+ T P + ++ + C+ P+ +W K+G I T D R + +N ++ +E+ D Y + + +G + + L V A+E
Sbjct: 362 LIINKVQPEDAGAYKVTLTNDLG-EASSEGKLTLSGAPQFQDTIEDQKTAVDDAWKIVTKVSG--NPELTW-YKNGVPITLDSRIKSIKVDPETFELVFAKTLADDNGNWAVIARNAHGEMSQFFEFSAQML----PKFETRLIDVEANEGKQAILKCKINCSPDPEVKWFK---GGTEITKDPRVKVYKDPNGLDCLTINSCSRGMAGEYEVKAINDMGTAACKCTIKVNTK----PSCDDLDDYEVFEKDDFTFAVECDGNPKPVCKWTKEGQSIDT-KDGRFVITENNGVYMLGIKEVTXDDKGXYXXEFTNRAGDRKVQSELIVHSADE 678
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000008465 (supercontig:LSalAtl2s:LSalAtl2s51:20931:201116:-1 gene:EMLSAG00000008465 transcript:EMLSAT00000008465 description:"maker-LSalAtl2s51-augustus-gene-2.12") HSP 1 Score: 86.2705 bits (212), Expect = 3.366e-17 Identity = 161/728 (22.12%), Postives = 291/728 (39.97%), Query Frame = 0
Query: 255 EARFLLSIEGEGTLIISQVQPK----DEGQYQCVAKNVAGIRLSAKARLSL-------QVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSR--------RNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAG----FVEASAHLQVFSPPVFLVKPTDQKGVQ-GSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEG--MLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQT-RVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLS---ITVQIWDPIVFH--LSSFQIEYYT--SRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQ-KFVTGYHIQLRD--ISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEALNFTL----EPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDL 941
+ R+L+S G+L+ +++P D+ +++C+A+N G +SA + L + P + +HP C G P + W + N L + G+ L + + D+ Y C+AEN G + PP F V+P D V GS++ + CV G+P+P + W K GS P G +L + + SE S YTC + S+ G I ++ V++Q ++ R P +++ V TP T R+ W G M T+ ++ L + K SG +++ F + E++ V L+ EP + G D + G +P ++ +VR PLS + +Q +P + ++ +++ Y T S + T W + V +++ T+ + P T + V A G PP S+ Q + G + S+ S+ RV+E++ + + L+W + + Y + D +D + S D+I+ ++TL L PNT Y V+ R +G T + V T++ VP A P+ VK + T+ + W PP +G + +++ + T+ P + ++ L + Y + + A G GP+ PV ++ D+
Sbjct: 160 QTRYLISEFRGGSLL--RIEPARRKFDDSKFECLAENGXGDPVSATSTLKVVEDNNLPTGFPEIKQHPGMKVVEKAHNALLVCQAAGNPSVNVYWVKDTMKLDVDSNPRLTILKQGKLRGS-LQIKDSQESDQGKYECVAENSVGTQYSYAAQVXVRVRRVPPTFSVQPDDVYNVMMGSSLNLTCVGVGSPMPYVKWRK---------GSQDMSALTPPIGRNVLELQDISE--SANYTCVAASKLGIIEHTTLVQVQ-ARPRAPTNVRLSDV----------------TPTTVRLSWSYPGNNAEVMY---------YTIQYK------PKLASWDYKEVSG--IITDFYDIRGLSPFTEYEFV-ILAVNNVGRGEPSTPVVATTGETDGRDSGKKPGSAP-RNVRVR--------PLSSSTMVIQXDEPQANNGQITGYKVYYTTNPSDSLTQWES-QFVDNNKLTTISELIPHTIYTIKVEAYXAIGPGPP---SEAVQVKTQQG--------VPSQPSNFRVIEVR------ATTMTLAWSSPSHSGENIVSYELYWNDTFTNDFQNQKSLDIIS-------NYTLEGLYPNTLYHVWXGAKXRRGEGATTPPIAVRTEQYVPGAPPDLVKAYAESSTSLRVVWSPPPPGKRNGKITYYEIYYVPSARPDSEATMVEIKNPEAREYVIDELRKYTEYRLWMLAGTSVGXGPISYPVVVKTGEDV 804 HSP 2 Score: 81.2629 bits (199), Expect = 1.189e-15 Identity = 155/688 (22.53%), Postives = 258/688 (37.50%), Query Frame = 0
Query: 305 PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHTTDGTQLTLMNIVPE----DEDVYTCLAENRAGF-VEASAHLQVFSP-------PVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEG-----GSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVN-QTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDI-----SDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLI---PQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEALNFTLEPSSNSLILYNLTEGL----TYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAPGMDLV 957
P + P++++ +G F C G P P I W R++G R ++ +L+ I P D+ + CLAEN G V A++ L+V P P + + + C AAGNP ++W+K+ S L QG + G L+I ++ E D G Y C + + G S ++ R PP + P + +GSS N C +P V+W K +S + P + N L + +IS+SA YTC A S G + ++V P +P+ L + T + + W P + + Y ++ S WD +S ++Y +++ FT Y+F V++ N+ P +P+ T G D S PR +R + M ++ + + +++ P +T + Q D + L T+++L P+T Y++ + Y G P++ V V T++ VP + P N ++ + T + W P H + N T +F + S + + Y L EGL Y V A R+G G P+ + E Q VP P DLV
Sbjct: 116 PEITDPPKDITVRSGGIAAFYCYASGDPKPHILW-RKDGEKISYSQTRYLISEFRGGSLLRIEPARRKFDDSKFECLAENGXGDPVSATSTLKVVEDNNLPTGFPEIKQHPGMKVVEKAHNALLVCQAAGNPSVNVYWVKDTMKLDVDSNPRLTILKQGKLR----GSLQIKDSQESDQGKYECVAENSVGTQY-SYAAQVXVRVRRVPPTFSVQPDDVYNVMMGSSLNLTCVGVGSPMPYVKWRKGSQDMSALTPPIGR----NVLELQ--DISESANYTCVAASKLGIIEHTTLVQVQAR---------------PRAPTNVRLSDVTPTTVRLSWSYP--GNNAEVMYYTIQYKPKLAS---------WD--YKEVSGIITDFY------------DIRGLSPFTEYEF-------VILAVNNVGRGEPSTPVVA--TTGETDGRDSGKKPGSAPRNVRVRPLSSSTMVIQXDEPQANNGQITGYKVYYTTNPSDSLTQWESQFVDNNKLT------------------TISELIPHTIYTIKVEAYXAIGPGPPSEAVQVKTQQGVP-SQPSNFRVIEVRATTMTLAWSSPS----HSGENIVSYELYWNDTFTNDFQNQKSLDIISNYTL-EGLYPNTLYHVWXGAKXRRGEGATTPPIAVRTE------QYVPGAPP--DLV 710 HSP 3 Score: 68.5514 bits (166), Expect = 7.374e-12 Identity = 74/326 (22.70%), Postives = 143/326 (43.87%), Query Frame = 0
Query: 128 PQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNS-----PSEEQKEKEK---LFEARKDEIGTESLTLSYC---------PKLDTRPQIELVVACNREDFDC-PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGE--GTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVL---PYVLKHPENV-SAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQK--GVQGSTVQIPCVAAGNPVPILFW 427
P+D+ GG A F C G P P W+KDG ++ + + + +G S P+ + + K L E + + + TL P++ P +++V + C G ++ + + ++L R + +G+ G+L I Q D+G+Y+CVA+N G + S A++ ++V P P++V + + G ++ C G P P ++W R G + G + + + E + YTC+A ++ G +E + +QV + P PT+ + V +TV++ GN ++++
Sbjct: 122 PKDITVRSGGIAAFYCYA-SGDPKPHILWRKDGE--KISYSQTRYLISEFRGGSLLRIEPARRKFDDSKFECLAENGXGDPVSATSTLKVVEDNNLPTGFPEIKQHPGMKVVEKAHNALLVCQAAGNPSVNVYWVKDTMKLDVDSNPRLTILKQGKLRGSLQIKDSQESDQGKYECVAENSVGTQYSYAAQVXVRVRRVPPTFSVQPDDVYNVMMGSSLNLTCVGVGSPMPYVKW--RKGSQDMSALTPPIGRNVLELQDISESAN-YTCVAASKLGIIEHTTLVQVQARP---RAPTNVRLSDVTPTTVRLSWSYPGNNAEVMYY 438 HSP 4 Score: 57.3806 bits (137), Expect = 2.032e-8 Identity = 38/150 (25.33%), Postives = 69/150 (46.00%), Query Frame = 0
Query: 794 VITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEALNFTL-------EPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELE 936
++ I R + + +LR TEY ++ML G + V+V T EDVP P N++ +N ++ + W P D +G ++G+++ + +E+ +T+ + L Y +QVAA R G G PV+++
Sbjct: 755 MVEIKNPEAREYVIDELRKYTEYRLWMLAGTSVGXGPISYPVVVKTGEDVP-GEPLNIRALAINSSSVQVHWDPPTDDDKNGVIRGYQIYVKPKSSESEXYTMPLRFITRNGKETEFNVTGLQPDTRYVIQVAALTRIGDGTRSKPVKVK 903 HSP 5 Score: 54.6842 bits (130), Expect = 1.557e-7 Identity = 40/140 (28.57%), Postives = 61/140 (43.57%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKD--KFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKK 158
P I +HP V + L C+A G+P + W KD + D +L G L ++ +E+D G Y C A N+ G S A + V V + F ++P DV + G+++ GSP P W+K
Sbjct: 219 PEIKQHPGMKVVEKAHNALLVCQAAGNPSVNVYWVKD---TMKLDVDSNPRLTILKQGKLRGSLQIKDSQESDQGKYECVAENSVGTQYSYAAQVXVRVRRVPPTFSVQPDDVYNVMMGSSLNLTCVGVGSPMPYVKWRK 355 HSP 6 Score: 52.7582 bits (125), Expect = 5.915e-7 Identity = 46/185 (24.86%), Postives = 86/185 (46.49%), Query Frame = 0
Query: 18 ASPPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLP---SGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKD------KFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQ--KIQGNSPSEEQKEKEK 191
++PP IT+ P++ +V C A GDP+P I W KDGE + + R L+ GSL LR ++++ D + C A N G S ++L V + + + P + A+ C G+P+ +W KD L+++ +P + ++ K++G+ ++ +E ++
Sbjct: 113 SNPPEITDPPKDITVRSGGIAAFYCYASGDPKPHILWRKDGEKI-----SYSQTRYLISEFRGGSL--LRIEPARRKFDDSKFECLAENGXGDPVSATSTLKVVEDNNLPTGFPEIKQHPGMKVVEKAHNALLVCQ-AAGNPSVNVYWVKDTMKLDVDSNPRLTILKQGKLRGSLQIKDSQESDQ 289
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000011416 (supercontig:LSalAtl2s:LSalAtl2s789:132799:170277:1 gene:EMLSAG00000011416 transcript:EMLSAT00000011416 description:"maker-LSalAtl2s789-snap-gene-0.23") HSP 1 Score: 84.3445 bits (207), Expect = 5.791e-17 Identity = 73/291 (25.09%), Postives = 122/291 (41.92%), Query Frame = 0
Query: 319 GDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVF-SPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEG-----GSGVLLPGS----NQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILA-SSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQ---TRVEWLKDGVPISTMNDPRIKV----DHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLR--VAPAEENVEF 589
G R C + R I W + LP+ T+ L + N+ ED +Y C AS+ L++ + PV + DQ G V + C + G+P P + W G ++ G ++ H+ V G L I KD+G Y C + + G + S ++K+ PP I V + R+ + C T + W ++ P+S D I + D+++ L ++L+ +A +TC AK+++G S +LR VAP E+ F
Sbjct: 11 GRKARLECLTESRDRDSIHWKKXGRXLPSHNRILTNKQTLQIFNLQREDYGMYQCFVRRDXYEAXASSELRLGDASPVLTYRFIDQTLQPGPLVSLKCTSRGSPTPGITWSLNGFPLPDNERFMMDGQKLPFDRRHL-VFDNGTLIISKVIRKDTGRYFCTASNRQGTSASQSGDLKVLV-----PPTINHFSVGEHLRLSQRVSMLCSITDGDLPISLTWYRNSEPLSDGGDG-ISLTQIGDYESVLRIDDLKPQHNANFTCVAKNSAGITQYSQYLRVKVAPVVESFTF 294 HSP 2 Score: 72.4034 bits (176), Expect = 3.089e-13 Identity = 87/345 (25.22%), Postives = 141/345 (40.87%), Query Frame = 0
Query: 267 TLIISQVQPKDEGQYQC-VAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGM--------------LPAQRVHTT-DGTQLTLMNIVPEDEDVYTCLAENRAGF-VEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPI-LFW------MKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQ--IGPVNQTQRIGSSTNFPC---EATPQTRVEWLKDGVPISTMNDPRI-KVD-HDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
TL I +Q +D G YQC V ++ S++ RL P + + + G V C +G P P I WS NG LP R H D L + ++ +D Y C A NR G S L+V PP + V + C +PI L W + +GG G+ L + G E +LRI + + + +TC + + +G S ++++ + P+++ P N TQ G C E V W KDG P+ + + RI ++D + + L +LE + Y+C A++ + S++L +
Sbjct: 49 TLQIFNLQREDYGMYQCFVRRDXYEAXASSELRLG-DASPVLTYRFIDQTLQPGPLVSLKCTSRGSPTPGITWS-LNGFPLPDNERFMMDGQKLPFDRRHLVFDNGTLIISKVIRKDTGRYFCTASNRQGTSASQSGDLKVLVPPTINHFSVGEHLRLSQRVSMLCSITDGDLPISLTWYRNSEPLSDGGDGISL--TQIGDY----ESVLRIDDLKPQHNANFTCVAKNSAGITQYSQYLRVKVA-----PVVESFTFPKNLTQ--GKRLRAICTIIEGDLPLDVSWSKDGSPLIDVPNVRITRIDEYTSLLAISKLESLHTGNYSCMAENAVAKHSTSSYLTI 378 HSP 3 Score: 65.855 bits (159), Expect = 3.542e-11 Identity = 82/334 (24.55%), Postives = 147/334 (44.01%), Query Frame = 0
Query: 265 EGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYV--LKHPENVSAVAGDTVRFGCAV-KGRPHPEIRWSRRNG-MLPAQRVHTTDGTQLTLMNIVPEDEDV----YTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPC-VAAGNPVPILFWMKEGGSGV---LLPGSNQGHVHVSPE----GMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPI--IQIGPVNQTQRIGSSTNFPCEATPQTRVE--WLKDG-----VPISTMNDPRIKVDH-DNTLHFEELEISDSALYTCQAKSTSGQA 572
E L I ++P+ + CVAKN AGI ++ L ++V P V P+N++ G +R C + +G ++ WS+ ++ V T + T + + + E + Y+C+AEN S++L + PP ++ KP D + V+G V C V+ N ++ G+ LL S+Q V+ + + G L I ++ D G + C +E+G +V + + PP +G + T R+ + C A+ + W KDG P + ++D + L E I+D+ Y+C A + G++
Sbjct: 248 ESVLRIDDLKPQHNANFTCVAKNSAGITQYSQY-LRVKVAPVVESFTFPKNLTQ--GKRLRAICTIIEGDLPLDVSWSKDGSPLIDVPNVRITRIDEYTSLLAISKLESLHTGNYSCMAENAVAKHSTSSYLTIKVPPEWVTKPHDLEAVEGQDVFFHCRVSEAN-------VRTNGNRRRIPLLELSSQRPVYRNYKLLINGTLYIRKVTDTDEGSFLCE--AENGVNEPLGQV-VHLKINAPPKFENSHVGVI--TGRMEEAIELQCVASGDDHISMTWRKDGRDYLTTPGKSRTTEIRELDKVTSKLRIENANINDAGKYSCLASNAFGKS 566 HSP 4 Score: 63.5438 bits (153), Expect = 1.756e-10 Identity = 60/236 (25.42%), Postives = 102/236 (43.22%), Query Frame = 0
Query: 254 PEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRR-------NGMLPAQRVHT--TDGTQLTLMNIVPEDEDVYTCLAENRAGF-VEASAHLQVFSPPVFLVKPTDQ-KGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGM---LRIGNTSEKDSGVYTCASVSESG 475
P R E L IS+++ G Y C+A+N + S + L+++V P + P ++ AV G V F C V + +RR + P R + +GT L + + DE + C AEN + HL++ +PP F G +++ CVA+G+ + W K+G + PG ++ + + LRI N + D+G Y+C + + G
Sbjct: 332 PNVRITRIDEYTSLLAISKLESLHTGNYSCMAENAVA-KHSTSSYLTIKVPPEWVTKPHDLEAVEGQDVFFHCRV-SEANVRTNGNRRRIPLLELSSQRPVYRNYKLLINGT-LYIRKVTDTDEGSFLCEAENGVNEPLGQVVHLKINAPPKFENSHVGVITGRMEEAIELQCVASGDDHISMTWRKDGRDYLTTPGKSRTTEIRELDKVTSKLRIENANINDAGKYSCLASNAFG 564 HSP 5 Score: 62.7734 bits (151), Expect = 2.770e-10 Identity = 72/320 (22.50%), Postives = 124/320 (38.75%), Query Frame = 0
Query: 266 GTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHP-EIRWSRRNGMLPAQRVHTTDGTQLTLM----------NIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPI-LFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT-PQTRVEWLKDGVPISTMNDPR-----IKVDHDNTLHFEELEISDSALYTCQAKS 567
GTLIIS+V KD G+Y C A N G S L + V P + V C++ P + W R + L DG LT + ++ P+ +TC+A+N AG + S +L+V PV + QG ++ C +P+ + W K+G + +P + +L I +G Y+C + + SS + I+ PP P + G F C + R + +P+ ++ R K+ + TL+ ++ +D + C+A++
Sbjct: 152 GTLIISKVIRKDTGRYFCTASNRQGTSASQSGDLKVLVPPTINHFSVGEHLRLSQRVSMLCSITDGDLPISLTWYRNSEPLS----DGGDGISLTQIGDYESVLRIDDLKPQHNANFTCVAKNSAGITQYSQYLRVKVAPVVESFTFPKNLTQGKRLRAICTIIEGDLPLDVSWSKDGSPLIDVPNVRITRID-EYTSLLAISKLESLHTGNYSCMAENAVAKHSTSSYLTIKV-----PPEWVTKPHDLEAVEGQDVFFHCRVSEANVRTNGNRRRIPLLELSSQRPVYRNYKLLINGTLYIRKVTDTDEGSFLCEAEN 461
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000004931 (supercontig:LSalAtl2s:LSalAtl2s259:474691:477217:1 gene:EMLSAG00000004931 transcript:EMLSAT00000004931 description:"maker-LSalAtl2s259-augustus-gene-5.6") HSP 1 Score: 82.0333 bits (201), Expect = 1.014e-16 Identity = 68/252 (26.98%), Postives = 117/252 (46.43%), Query Frame = 0
Query: 257 RF-LLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQV-LPYVLKH--PENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAG-FVEASAHLQV-FSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGV----LLPGSNQGHVHVSPEG-----MLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPP 493
RF L S +G L I +++ D +YQC + V + A +S++V LP V+ P NV +V G+ V C G P P+I W R +G L + G LT+ + D Y C A N G VE + + ++ F+P +++ +P + + G V++ C + P P + W + + + G+N H + + L+I +E+ G Y C +V++ G+ + + E+ T PP
Sbjct: 80 RFHLKSEDGTYILTIEELKESDASKYQC--QVVVSLNDKKTADVSVKVKLPPVVSDDTPVNVQSVEGEGVDLICEASGFPTPKITWRRMDGALMFNGNNEVIGNILTIKKVQRGDRGTYICSASNGVGDDVEHAIYFEIGFTPEIYIDRPRTSQAL-GYDVELTCKISAFPTPGITWYRLSNNSTDNIRIDEGNNYQLSHFAQDNGLFVTQLKIIGITEEQFGSYICKAVNKFGSAVQTIELYTSTLPICPP 328
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000011259 (supercontig:LSalAtl2s:LSalAtl2s770:259976:262330:-1 gene:EMLSAG00000011259 transcript:EMLSAT00000011259 description:"maker-LSalAtl2s770-snap-gene-2.21") HSP 1 Score: 79.337 bits (194), Expect = 3.007e-15 Identity = 99/444 (22.30%), Postives = 168/444 (37.84%), Query Frame = 0
Query: 213 LDTRPQIELVVACNREDFDCPKGL--EAIMGNFGRQLI---RLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVS--AVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDED-VYTCLAENRAGFVEASAHLQVFSPPV----------FLVKPTDQKGV-QGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAE---------------ENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWD 620
+ RPQ + + F+C + E + + QLI L ++ LS E + + K G+YQC+ + + A LS+ L +N S G+ V C V P R+G+ V + L + N+VPE + YTC +N + + QV + + P + V +G ++ IPC A+G P P + W + G S ++P S+ G+L+I N +E++ G Y C + L + + + T PP ++ ++T R G S + C P + W +G P+ D R N L+ + S S Y C + +RV + E ++ D+ FL PS+P + + + +I WD
Sbjct: 22 FEERPQNSVANPGDTVYFNCKTNISEEFVQWTYKGQLISAQELSVYTISKNYLSFE---LGLTKDIYSKQIGEYQCIGRESIA---TLPANLSIAELKEFSSEGKNSSFEVTQGNDVIIHCDVPYSNPPGFVEYYRDGIYLEAEV-LLNSEILFIKNVVPELHNGNYTCSVQNHIIGQKKHSKFQVELKVIPSHGNRRKSQIIYHPKGKYSVNKGESITIPCAASGFPKPFISWSRFGDSXAIVPSSH---------GLLQIRNATEENQGDYMCIVDDGARQFLRKTRLIVNT----PPVFLKTPQTHKTLRDGDSLSLKCIGSGVPSPEIRWYHNGFPLL---DQR-----SNHLYIHSVNNSHSGFYQCFIINIVNANYTVTLVRVLQNKNEGHDYNEDYEDYPLEVLQVDAATFL-----PPSKPNIXQLSPDSAMISWD 432
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000011915 (supercontig:LSalAtl2s:LSalAtl2s840:73:4762:1 gene:EMLSAG00000011915 transcript:EMLSAT00000011915 description:"maker-LSalAtl2s840-augustus-gene-0.11") HSP 1 Score: 78.1814 bits (191), Expect = 4.380e-15 Identity = 125/509 (24.56%), Postives = 195/509 (38.31%), Query Frame = 0
Query: 57 DGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWC--EATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELEL-----------DPSICWVQ--KIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPE-IRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENR---------AGFVEASAHLQV-FSPPVFLVKPTDQKGVQGSTVQIPCVAA--GNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGA-ILASSEVKI---QTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEATPQTRVEWLKDGVPIS 533
D + +HK +RV L + LR + + + D G + C + T + S++ L V + I + C GSP PT W + GR L SI ++ K NS Q L E K E T+ + ++Y P++ P+ L V + D LE + + R + + E RF I + I +V D G Y C A N G ++ +L + P V +V A G+ V C V P P I+W + + +G LTL +I D+ Y C A NR + +S + V P ++P V+G ++ + C A G P P W K+G +L GS + V IG+ ++G Y C +E G+ LA EVK+ Q + R P +Q + + + G + P+ +V W KDG I+
Sbjct: 39 DNXAIGQTPYEHK-YRVELSDEGTYNLRIINATYDRDNGDFECRMKKGGTGETLHSKSVKLTVLLKPSPPEIIIDAPSVTEDQLTNLTCSSIGGSPPPTMEWFRQGRSTPLNALSIPGANKDVPTKSILTIKPSKEDDNSSYRCQIWNRALKEQNKLETSTK-IHVNYFPRVTVGPENPLKV-----EKDETASLECKVDSKPR-VNSVKWTREGRF---INTDFKHTIPRVSLSDAGSYLCSADNNLGRVGESELKLDVLYAP-VFNLSRSVEASEGEEVNIECHVSSNPRPSSIQWYKE-----GDKDFLHNGRILTLNSITASDDGEYICSATNRIQPTGKPEYSRTRNSSVIINVRHKPGKAFIQPEKPIAVEGKSITLTCGANPPGFPTPRYTWFKDGNQ--ILSGSGKNPDFV-------IGSADLDNAGKYECRPANELGSGTLA--EVKLLVYQAPKIRTP--LQPTIMKRARDTGFHSTCSAVGKPKPKVHWFKDGREIT 517
BLAST of roundabout 1 vs. L. salmonis genes
Match: EMLSAG00000006099 (supercontig:LSalAtl2s:LSalAtl2s331:186688:204861:-1 gene:EMLSAG00000006099 transcript:EMLSAT00000006099 description:"augustus_masked-LSalAtl2s331-processed-gene-2.0") HSP 1 Score: 76.6406 bits (187), Expect = 1.205e-14 Identity = 63/217 (29.03%), Postives = 96/217 (44.24%), Query Frame = 0
Query: 268 LIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVA--GDTVRFGCAVKGRPHPEIRWSRRNGMLPAQ--RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGF--VEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEG---MLRIGNTSEKDSGVYTCASVSESG 475
L IS+++ D G+Y C + A + + + + V P + P N V G TV C V G P P I+W + + LP RV + LTL ++ VY C+A+N G VE HLQV P + G G V++ CV P + W K+ +L SN G ++ +G L I + + D G Y+C++ + G
Sbjct: 134 LEISELKKSDGGEYICELETDADLPSTITHTVEILVPPKLRTSPSNGQIVVKKGTTVSLTCHVTGXPEPVIKWEKLHERLPKGDIRVEERLSSTLTLSDVTRHHAGVYKCIADNGVGDSPVEQEIHLQVLYEPEAKPEQLQIHGGIGYQVELVCVVYAEPQADVLWYKD----TMLLDSN-GKRYMQQKGNRFTLLIKDVEKSDFGNYSCSASNMLG 345
BLAST of roundabout 1 vs. SwissProt
Match: gi|449061888|sp|G5EBF1.1|SAX3_CAEEL (RecName: Full=Protein sax-3; AltName: Full=Sensory axon guidance 3; Flags: Precursor) HSP 1 Score: 376.326 bits (965), Expect = 3.044e-111 Identity = 233/711 (32.77%), Postives = 355/711 (49.93%), Query Frame = 0
Query: 261 SIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVH-TTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG--SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT------SQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTP-PKAGEDISDPRYLRSKLSDKR-----VMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNG---RIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDING-TEALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEK 950
++ +G LII V D G YQCVA N+ G R+S ARLS+ P + P++++ G V F C V G P P+I W R+N +P R + D L + + P DE Y C A N AG +EASAHL+V +PP F KP DQ G T C G P P FW KEG +L P S G VSP G L I + D G Y CA ++ +G+ L+ + +K+ T + +PPP I+ G NQT +GSS PC+A+ P + WL+DG+PI + D RI +LH +L+ D+ +YTC AK+ G++ SA L V N +F +P S FPSSP++P ++N T ++ + W+ P G PI Y I+YY+ W IP+ +S + + +P+ + ++RA N G+ PS S T P A +SD + +++KR +++L+EVK + S V+L W+ ++ + GY+I+ R + + +V + S T ++ +++L P T Y F++PY+ I G P+ + V T E P+ PE+V+I+MLNLT I W P ++G LKGF+++I N T + S+ L++L G+TY ++VAA + GVG E+ + D L ++
Sbjct: 183 TLHSDGNLIIDPVDRSDSGTYQCVANNMVGERVSNPARLSVFEKPKFEQEPKDMTVDVGAAVLFDCRVTGDPQPQITWKRKNEPMPVTRAYIAKDNRGLRIERVQPSDEGEYVCYARNPAGTLEASAHLRVQAPPSFQTKPADQSVPAGGTATFECTLVGQPSPAYFWSKEGQQDLLFPSYVSADGRTKVSPTGTLTIEEVRQVDEGAYVCAGMNSAGSSLSKAALKVTTKAVTGNTPAKPPPTIEHGHQNQTLMVGSSAILPCQASGKPTPGISWLRDGLPID-ITDSRISQHSTGSLHIADLKKPDTGVYTCIAKNEDGESTWSASLTVEDHTSNAQFVRMPDPSNFPSSPTQPIIVNVTDTEVELHWNAPSTSGAGPITGY------------------------------IIQYYSPDLGQTWFNIPDYVASTEYRIKGLKPSHSYMFVIRAENEKGIGTPSVSSALVTTSKPAAQVALSDKNKMDMAIAEKRLTSEQLIKLEEVKTINSTAVRLFWKKRKLEELIDGYYIKWRGPPRTNDNQYVNVTSPS---TENYVVSNLMPFTNYEFFVIPYHSGVHSIHGAPSNSMDVLTAEAPPSLPPEDVRIRMLNLTTLRISWKAPKADGINGILKGFQIVIVGQAPNNNRNITTNERAASVTLFHLVTGMTYKIRVAARSNGGVGVSHGTSEVIMNQDTLEKHLAAQQ 859 HSP 2 Score: 217.624 bits (553), Expect = 9.747e-57 Identity = 161/515 (31.26%), Postives = 245/515 (47.57%), Query Frame = 0
Query: 8 VLFSFPGCWAAS--PPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSK--KENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGN---SPSEEQKEKEKLFEARKDEIGTE------SLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKR---PEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSR---RNGMLPAQ-----RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----------PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLP-GSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ 486
V+ SF C AS PVI EHP + V+R P TL C A+ +I W+KDG+PV+T SHR++L +GSLF L+ K K++D G Y+C A+N G V+S SL +A+L++ FR+ P+ V A+ G A+ EC PP+G P P W+KD + L ++ P + GN P + + ++ + + E L++ PK + P+ V FDC + G+ Q+ K P R ++ + G L I +VQP DEG+Y C A+N AG L A A L +Q P P + S AG T F C + G+P P WS+ ++ + P+ R + LT+ + DE Y C N AG + A L+V + PP +Q + GS+ +PC A+G P P + W+++G LP + G L I + + D+GVYTC + +E G S+ + ++
Sbjct: 18 VIRSF--CEDASNLAPVIIEHPIDVVVSRGSPATLNCGAK-PSTAKITWYKDGQPVITNKEQVNSHRIVLDTGSLFLLKVNSGKNGKDSDAGAYYCVASNEHGEVKSNEGSLKLAMLREDFRVRPRTVQALGGEMAVLECSPPRGFPEPVVSWRKDDKELRIQDMPR--YTLHSDGNLIIDPVD--RSDSGTYQCVANNMVGERVSNPARLSVFEKPKFEQEPKDMTVDVGAAVLFDC-----RVTGDPQPQITWKRKNEPMPVTRAYIAKDNRG-LRIERVQPSDEGEYVCYARNPAGT-LEASAHLRVQAPPSFQTKPADQSVPAGGTATFECTLVGQPSPAYFWSKEGQQDLLFPSYVSADGRTKVSPTGTLTIEEVRQVDEGAYVCAGMNSAGSSLSKAALKVTTKAVTGNTPAKPPPTIEHGHQNQTLMVGSSAILPCQASGKPTPGISWLRDG-----LPIDITDSRISQHSTGSLHIADLKKPDTGVYTCIAKNEDGESTWSASLTVE 513 HSP 3 Score: 105.916 bits (263), Expect = 7.682e-22 Identity = 90/323 (27.86%), Postives = 133/323 (41.18%), Query Frame = 0
Query: 277 DEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLK-HPENVSAVAGDTVRFGCA-VKGRPHPEIRWSRRNGML-----PAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAG-FVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGG----SGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCE--ATPQTRVEWLKDG-----VPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
D G Y CVA N G S + L L +L + P V A+ G+ C+ +G P P + W + + L P +H +DG L + + D Y C+A N G V A L VF P F +P D G+ V C G+P P + W ++ + + N+G LRI D G Y C + + +G + AS+ +++Q PP Q P +Q+ G + F C P W K+G P D R KV TL EE+ D Y C +++G ++ A L+V
Sbjct: 104 DAGAYYCVASNEHGEVKSNEGSLKLAMLREDFRVRPRTVQALGGEMAVLECSPPRGFPEPVVSWRKDDKELRIQDMPRYTLH-SDG-NLIIDPVDRSDSGTYQCVANNMVGERVSNPARLSVFEKPKFEQEPKDMTVDVGAAVLFDCRVTGDPQPQITWKRKNEPMPVTRAYIAKDNRG---------LRIERVQPSDEGEYVCYARNPAGTLEASAHLRVQA-----PPSFQTKPADQSVPAGGTATFECTLVGQPSPAYFWSKEGQQDLLFPSYVSADGRTKVSPTGTLTIEEVRQVDEGAYVCAGMNSAGSSLSKAALKV 410
BLAST of roundabout 1 vs. SwissProt
Match: gi|49036496|sp|Q9HCK4.2|ROBO2_HUMAN (RecName: Full=Roundabout homolog 2; Flags: Precursor) HSP 1 Score: 345.51 bits (885), Expect = 1.118e-99 Identity = 262/910 (28.79%), Postives = 408/910 (44.84%), Query Frame = 0
Query: 128 PQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDP-----------SICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDF-----------------DC--PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQ---GHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKI-QTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQT----PPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQML---NLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTE-ALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEV--ETDLFHPQLVPEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDG 991
P DV +G C +G PTPT W KDG +E + D S+ +++ + G ++ DE + +Y + +R L VA R+DF +C P+G + + +R+ + E +SI G G L+IS + D G Y CV N+ G R S A L++ P L+ P N + + V F C V+G P P +RW + + LP R D L + + DE Y C+AENR G +EASA L V +PP F+V+P DQ QG TV PC GNP P +FW KEG +L P Q VSP G L I N D+G Y C +++ +G+ILA +++++ DRPPPII GP NQT + + C+AT P + WLK+G DPR + TL + L ISD+ YTC A S+SG+ SA L V E LS+ P PS+P + + T N + + W Q P S++ IE ++ W + + +T+ P T +VRA N GLS PSP+S P +T PP G D R ++ +L D ++ L +L V+++W + +F+ GY + R S L ++ S+ + RS L +L+ Y + + PY +G ++ V T E+ P+A P++V + + N T+ + W PP +G ++ +K+ N T +N T++ + S+I+ L G+ Y V+VAA GVG +P + + ++ + + VV++ F+ + ++L+GF + +RR ++ G
Sbjct: 37 PSDVIVSKGEPTTLNCKA-EGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGR-------------RSKPDEGSYVCVARNYLGEAVSR-NASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEER---ISIRG-GKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPG-RDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDV--TESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSW-------------------------------QPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQG---VDHRQVQKELGDV-LVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKG 889 HSP 2 Score: 255.758 bits (652), Expect = 5.129e-69 Identity = 174/496 (35.08%), Postives = 244/496 (49.19%), Query Frame = 0
Query: 20 PPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKEN-DGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDG-RILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTE------SLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKR----PEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSR---RNGMLPAQ------RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS------PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTS 488
PP I EHP + V++ EP TL C+A+G P P I+W+KDGE V T D +SHR+LLPSGSLFFLR V ++ D G Y C A N G SRNASL VA+L+D FR P DV G AI EC PP+G P PT +WKKD RI + E SI + + N+ +K ++ + E LT+ P RP ++V+ +F C + G+ + +R K P R+ I+ + TL I + DEG Y C+A+N G ++ A A L+++ P + P + G TV F C KG P P + W + +N + P Q R + LT+ NI D Y C A AG + A A L+V PP+ L P +Q T + C A G+P+P++ W+KE G PG + + +G L+I N D+G YTC + S SG S+ + + S
Sbjct: 30 PPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNT----RKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRC-----QVQGD-PQPTVRWKKDDADLPRGRY--DIKDDYTLRIKKTMSTDEGTYMCIAENRVG-KMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKE---GFTFPGRDP-RATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTES 508 HSP 3 Score: 76.2554 bits (186), Expect = 8.931e-13 Identity = 79/299 (26.42%), Postives = 121/299 (40.47%), Query Frame = 0
Query: 305 PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPE----------------DEDVYTCLAENRAG-FVEASAHLQV-FSPPVFLVKPTDQKGVQGSTVQIPCV-AAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVD--HDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
P +++HP +V G+ C +GRP P I W + +RV T + ++P DE Y C+A N G V +A L+V F PTD G + C G+P P ++W K+ + + + + G L I NT + D+G+YTC + G S+ T +RP + + P+NQ + F C + PQ V W KD + PR + D D TL ++ +D Y C A++ G+ SA L V
Sbjct: 31 PRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKD-----GERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDK----VRIDDKEERISIR-GGKLMISNTRKSDAGMYTCVGTNMVGE--RDSDPAELTVFERPTFLRR--PINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADL-----PRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTV 310
BLAST of roundabout 1 vs. SwissProt
Match: gi|49036483|sp|Q7TPD3.2|ROBO2_MOUSE (RecName: Full=Roundabout homolog 2; Flags: Precursor) HSP 1 Score: 341.273 bits (874), Expect = 8.586e-98 Identity = 261/914 (28.56%), Postives = 409/914 (44.75%), Query Frame = 0
Query: 128 PQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDP-----------SICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDF-----------------DC--PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQV----FSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQ---GHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKI-QTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQT----PPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQML---NLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTE-ALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEV--ETDLFHPQLVPEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDG 991
P DV +G C +G PTPT W KDG +E + D S+ +++ + G ++ DE + +Y + +R L VA R+DF +C P+G + + +R+ + E +SI G G L+IS + D G Y CV N+ G R S A L++ P L+ P N + + V F C V+G P P +RW + + LP R D L + + DE Y C+AENR G VEASA L V +PP F+V+P DQ QG TV PC GNP P +FW KEG +L P Q VSP G L I N D+G Y C +++ +G+ILA +++++ DRPPPII GP+NQT + + C+AT P + WLK+G DPR + TL + L ISD+ YTC A S+SG+ SA L V E +++ P PS+P + + + N + + W Q P V S++ IE ++ W + + +T+ P T +VRA N GLS PSP+S P +T PP G D R ++ +L D V+ L +L V+++W + +F+ GY + R S L +S + + RS L +L+ Y + + PY +G ++ V T E+ P+A P++V + + N T+ + W PP +G ++ +K+ N T +N T++ + S+++ L G+ Y V+VAA GVG +P + + ++ + + VV++ F+ + ++L+GF + +RR ++ G
Sbjct: 37 PSDVIVSKGEPTTLNCKA-EGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGR-------------RSKPDEGSYVCVARNYLGEAVSR-NASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEER---ISIRG-GKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTF-LGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDV--TESGATISKNYDMNDLPGPPSKPQVTDVSKNSVTLSW-------------------------------QPGTPGVLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQG---VDHRQVQKELGDV-VVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQASTVWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSHNSTSISVSWDPPPADHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVVIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGGRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKG 893 HSP 2 Score: 246.514 bits (628), Expect = 6.177e-66 Identity = 173/500 (34.60%), Postives = 243/500 (48.60%), Query Frame = 0
Query: 20 PPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKEN-DGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDG-RILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTE------SLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKR----PEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYV----LKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSR---RNGMLPAQ------RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS------PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTS 488
PP I EHP + V++ EP TL C+A+G P P I+W+KDGE V T D +SHR+LLPSGSLFFLR V ++ D G Y C A N G SRNASL VA+L+D FR P DV G AI EC PP+G P PT +WKKD RI + E SI + + N+ +K ++ + E LT+ P RP ++V+ +F C + G+ + +R K P R+ I+ + TL I + DEG Y C+A+N G ++ A A L+++V P + P + G TV F C KG P P + W + +N + P Q R + LT+ NI D Y C A AG + A A L+V PP+ L P +Q T + C A G P+P++ W+KEG + + + +G L+I N D+G YTC + S SG S+ + + S
Sbjct: 30 PPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNT----RKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRC-----QVQGD-PQPTVRWKKDDADLPRGRY--DIKDDYTLRIKKAMSTDEGTYVCIAENRVG-KVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFL----GRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTES 512 HSP 3 Score: 93.9745 bits (232), Expect = 4.283e-18 Identity = 106/404 (26.24%), Postives = 161/404 (39.85%), Query Frame = 0
Query: 25 EHPQNSSVTRNEPVTLRCEA-QGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRIL-ELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEI-GTESLTLSYC----PKLDTRPQIELVVACNREDFDCPKGLEAIMGNF----GRQLIRLGKRPEA-RFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVL------PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQ--RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQG 408
++P + V EP L C+ +G PEP I W KD D K R+ + G L + + +++D G+Y C TN G S A L V + F P + ++ F C +G P PT WKKD L D + +I+ + S ++ + E R ++ + +LT+ P+ RP+ ++V F C F G Q + +P+ S+ G L I+ +Q D G Y C A VAG L AKA+L + + P +L+ P N + T C G P P I W + + R D L + N+ D YTC+A + +G SA L V + K D + G
Sbjct: 137 QNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKD-----KVRIDDKEERISIRGGKLM----ISNTRKSDAGMYTCVGTNMVGERDSDPAELTV-FERPTFLRRPINQVVLEEEAVEFRCQ-VQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIK-KAMSTDEGTYVCIAENRVGKVEASATLTVRVRPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSIL-AKAQLEVTDVLTDRPPPIILQGPINQTLAVDGTALLKCKATGEPLPVISWLKEGFTFLGRDPRATIQDQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDMNDLPG 527 HSP 4 Score: 77.0258 bits (188), Expect = 6.426e-13 Identity = 79/299 (26.42%), Postives = 121/299 (40.47%), Query Frame = 0
Query: 305 PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPE----------------DEDVYTCLAENRAG-FVEASAHLQV-FSPPVFLVKPTDQKGVQGSTVQIPCV-AAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPC--EATPQTRVEWLKDGVPISTMNDPRIKVD--HDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
P +++HP +V G+ C +GRP P I W + +RV T + ++P DE Y C+A N G V +A L+V F PTD G + C G+P P ++W K+ + + + + G L I NT + D+G+YTC + G S+ T +RP + + P+NQ + F C + PQ V W KD + PR + D D TL ++ +D Y C A++ G+ SA L V
Sbjct: 31 PRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKD-----GERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDK----VRIDDKEERISIR-GGKLMISNTRKSDAGMYTCVGTNMVGE--RDSDPAELTVFERPTFLRR--PINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADL-----PRGRYDIKDDYTLRIKKAMSTDEGTYVCIAENRVGKVEASATLTV 310
BLAST of roundabout 1 vs. SwissProt
Match: gi|49036480|sp|O89026.1|ROBO1_MOUSE (RecName: Full=Roundabout homolog 1; Flags: Precursor) HSP 1 Score: 312.768 bits (800), Expect = 7.960e-88 Identity = 246/895 (27.49%), Postives = 399/895 (44.58%), Query Frame = 0
Query: 128 PQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDP-----------SICWVQKIQGNSPSEEQK-----EKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDC--PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLL---PGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKI-QTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTP--PKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQML--NLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTE-ALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDP--VELEVETDLFHPQLVPEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDG 991
P D+ +G A C +G PTPT W K G +E + D S+ +++ + G ++ + L EA E L P +V +C P+G ++ + L + E ++I G G L+I+ + D G+Y CV N+ G R S A L++ P +K P N++ D+ F C +G P P +RW + +G LP R D L + + D YTC+AEN G EASA L V PP F+VKP DQ G TV C A GNP P +FW +EG +L P + VS G L I N D G Y C +++ +G+I+ + +++ DRPPP+I+ GPVNQT + + C AT P + W KDGV +ST D RIK L ++ D+ YTC A + SG+A SA++ V V+ + PS+PS+P + + + N + + W + G +P +S+ IE ++ + + W E +E F + +P LVRA N +G+S PS IS P +T P + + D + ++ +L + V+ L IL S V++ W + +++ GY I R + V + S + DLR Y + P+ +G +++ T E+ P+A P +V + N TA ++ W PP +G ++ +KV N T+ +N T++ S+ S+++ +L G+ Y+V+VAA G G +P ++L+ + P+ A + VV++ F+ + I+L+ F + R +++G
Sbjct: 35 PSDLIVSKGEPATLNCKA-EGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILR--DDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDER---ITIRG-GKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDGVLVSTQ-DSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLSWQPNLNSGATP-------------------------------TSYIIEAFSHASGSSWQTAAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTSQGV-DHKQVQRELGNV-VLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETKYHINKTVDGSTFSVVIPSLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVRQPAFIAGIGAACWIILMVFSIWLYRHRKKRNG 888 HSP 2 Score: 245.358 bits (625), Expect = 1.600e-65 Identity = 166/488 (34.02%), Postives = 235/488 (48.16%), Query Frame = 0
Query: 20 PPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKEN-DGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEK-----EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSR---RNGMLPAQ------RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS------PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ 486
PP I EHP + V++ EP TL C+A+G P P I+W+K GE V T D +SHR+LLPSGSLFFLR V +K D G+Y C A N G S NASL VA+L+D FR P DV G A+ EC PP+G P PT WKKDG L+ + + K+ + K + R+ E+ LT+ P RP V + +F C + + R+ G+ P++R+ I + TL I +V D G Y CVA+N+ G + A A L++Q P+ + P + G TV F C G P P I W R +N + Q R + LT+ N+ D Y C N AG + A+L+V PPV P +Q T+ + CVA G+P P + W K+G +L + + G+L+I D+G YTC + + SG S+ +++Q
Sbjct: 28 PPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVA--ELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRK--DDGELPKSRY--EIRDDHTLKIRKVTAGDMGSYTCVAENMVG-KAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVATGSPAPTILWRKDG----VLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQ 504
BLAST of roundabout 1 vs. SwissProt
Match: gi|49036500|sp|Q9Y6N7.1|ROBO1_HUMAN (RecName: Full=Roundabout homolog 1; AltName: Full=Deleted in U twenty twenty; AltName: Full=H-Robo-1; Flags: Precursor) HSP 1 Score: 308.531 bits (789), Expect = 2.370e-86 Identity = 244/897 (27.20%), Postives = 397/897 (44.26%), Query Frame = 0
Query: 128 PQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDP-----------SICWVQKIQGNSPSEEQK-----EKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDC--PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLL---PGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKI-QTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQT----PPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQML--NLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTE-ALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDP--VELEVETDLFHPQLVPEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDG 991
P D+ +G A C +G PTPT W K G +E + D S+ +++ + G ++ + L EA E L P +V +C P+G ++ + L + E ++I G G L+I+ + D G+Y CV N+ G R S A L++ P +K P N++ D+ F C +G P P +RW + +G LP R D L + + D YTC+AEN G EASA L V PP F+VKP DQ G TV C A GNP P +FW +EG +L P + VS G L I N D G Y C +++ +G+I+ + +++ DRPPP+I+ GPVNQT + + C AT P + W KDGV +ST D RIK + L ++ D+ YTC A + SG+A SA++ V V+ + PS+PS+P + + + N + + W + G +P +S+ IE ++ + + W + E +E + +P LVRA N +G+S PS IS P +T P G D + ++ +L + V+ L +L S +++ W + +++ GY I R + V + S + DLR Y + P+ +G +++ T E+ P+A P+ V + N TA ++ W PP +G ++ +KV N T +N T++ S+ S+++ L G+ Y+V+VAA G G +P ++L+ + P+ A + VV++ F+ + I+L+ F + R +++G
Sbjct: 74 PSDLIVSKGEPATLNCKA-EGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILR--DDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDER---ITIRG-GKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQ-DSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATP-------------------------------TSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQG---VDHKQVQRELGNA-VLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNG 927 HSP 2 Score: 246.128 bits (627), Expect = 9.838e-66 Identity = 167/488 (34.22%), Postives = 235/488 (48.16%), Query Frame = 0
Query: 20 PPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKEN-DGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEK-----EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSR---RNGMLPAQ------RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS------PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ 486
PP I EHP + V++ EP TL C+A+G P P I+W+K GE V T D +SHR+LLPSGSLFFLR V +K D G+Y C A N G S NASL VA+L+D FR P DV G A+ EC PP+G P PT WKKDG L+ + + K+ + K + R+ E+ LT+ P RP V + +F C + + R+ G+ P++R+ I + TL I +V D G Y CVA+N+ G + A A L++Q P+ + P + G TV F C G P P I W R +N + Q R + LT+ N+ D Y C N AG + A+L+V PPV P +Q T + CVA G+PVP + W K+G +L + + G+L+I D+G YTC + + SG S+ +++Q
Sbjct: 67 PPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVA--ELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRK--DDGELPKSRY--EIRDDHTLKIRKVTAGDMGSYTCVAENMVG-KAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDG----VLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQ 543
BLAST of roundabout 1 vs. SwissProt
Match: gi|49036462|sp|O55005.1|ROBO1_RAT (RecName: Full=Roundabout homolog 1; Flags: Precursor) HSP 1 Score: 303.908 bits (777), Expect = 7.948e-85 Identity = 243/895 (27.15%), Postives = 396/895 (44.25%), Query Frame = 0
Query: 128 PQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDP-----------SICWVQKIQGNSPSEEQK-----EKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDC--PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLL---PGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKI-QTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTP--PKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQML--NLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTE-ALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDP--VELEVETDLFHPQLVPEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDG 991
P D+ +G A C +G PTPT W K G +E + D S+ +++ + G ++ + L EA E L P +V +C P+G ++ + L + E ++I G G L+I+ + D G+Y CV N+ G R S A +++ P +K P N++ D+ F C +G P P W + +G LP R D L + + D YTC+AEN G EASA L V PP F+VKP DQ G TV C A GNP P +FW +EG +L P + VS G L + N D G Y C +++ +G+I+ + +++ DRPPP+I+ GPVNQT + + C AT P + W KDGV +ST D RIK L ++ D+ YTC A + SG+A SA++ V V+ + PS+PS+P + + + N + + W + G +P +S+ IE ++ + + W + E +E F + +P LVRA N +G+S PS IS P +T P + + D + ++ +L + V+ L IL S V++ W + +++ GY I R + V + S + DLR Y + P+ +G +++ T E+ P+A P +V + N TA ++ W PP +G ++ +KV N T +N T++ S+ S+++ L G+ Y+V+VAA G G +P ++L+ + P+ A + VV++ F+ + I+L+ F + R +++G
Sbjct: 74 PSDLIVSKGEPATLNCKA-EGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILR--DDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDER---ITIRG-GKLMITYTRKSDAGKYVCVGTNMVGERESKVADVTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTFGWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTVTNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLTLSCVATGSPVPTILWRKDGVLVSTQ-DSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSKNTVTLLWQPNLNSGATP-------------------------------TSYIIEAFSHASGSSWQTVAENVKTETFAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVPPTTQGV-DHKQVQRELGNV-VLHLHNPTILSSSSVEVHWTVDQQSQYIQGYKILYRPSGASHGESEWLVFEVRTPTKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEERPSAPPRSVTVSKNDGNGTAILVTWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGPGVKSEPQFIQLDSHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNG 927 HSP 2 Score: 245.743 bits (626), Expect = 1.146e-65 Identity = 166/488 (34.02%), Postives = 237/488 (48.57%), Query Frame = 0
Query: 20 PPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKEN-DGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEK-----EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSR---RNGMLPAQ------RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS------PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ 486
PP I EHP + V++ EP TL C+A+G P P I+W+K GE V T D +SHR+LLPSGSLFFLR V +K D G+Y C A N G S NASL VA+L+D FR P DV G A+ EC PP+G P PT WKKDG L+ + + K+ + K + R+ ++ +T+ P RP V + +F C + + FG + G+ P++R+ I + TL I +V D G Y CVA+N+ G + A A L++Q P+ + P + G TV F C G P P I W R +N + Q R + LT+ N+ D Y C N AG + A+L+V PPV P +Q T+ + CVA G+PVP + W K+G +L + + G+L+I D+G YTC + + SG S+ +++Q
Sbjct: 67 PPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESKVA--DVTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVP-TFGWRKDD-GELPKSRY--EIRDDHTLKIRKVTAGDMGSYTCVAENMVG-KAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTVTNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLTLSCVATGSPVPTILWRKDG----VLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQ 543 HSP 3 Score: 108.612 bits (270), Expect = 1.426e-22 Identity = 105/400 (26.25%), Postives = 170/400 (42.50%), Query Frame = 0
Query: 25 EHPQNSSVTRNEPVTLRCEA-QGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRIL-----ELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQ-----LIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVL------PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPA---QRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQV--FSPPVFLVKPTD 402
++P + V EP + C+ +G PEP I W KDG P+ D K R+ + G L + +++D G Y C TN G S+ A + V + + F P ++ +A F+C +G P PT W+KD L E+ D ++ + G+ S + + +A + +LT+ P +P+ ++V F C F R+ L+ + P++ S+ G L ++ VQ D G Y C NVAG + KA L + + P + + P N + T+ C G P P I W R++G+L + R+ + L + D YTC A +G SA+++V F PV +PTD
Sbjct: 174 QNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPL-----DDKDERITIRGGKLM----ITYTRKSDAGKYVCVGTNMVGERESKVADVTV-LERPSFVKRPSNLAVTVDDSAEFKC-EARGDPVPTFGWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAE----ASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTVTNVQRSDVGYYICQTLNVAG-SIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLTLSCVATGSPVPTILW-RKDGVLVSTQDSRIKQLESGVLQIRYAKLGDTGRYTCTASTPSGEATWSAYIEVQEFGVPVQPPRPTD 556 HSP 4 Score: 77.411 bits (189), Expect = 4.880e-13 Identity = 78/299 (26.09%), Postives = 117/299 (39.13%), Query Frame = 0
Query: 305 PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPE----------------DEDVYTCLAENRAGFVEASAHLQVFSPPV----FLVKPTDQKGVQGSTVQIPCVAA-GNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
P +++HP ++ G+ C +GRP P I W + +RV T + ++P DE VY C+A N G EA +H + F P+D G + C G+P P + W K+G P ++ G L I T + D+G Y C + G S+V T +RP + + P N + S F CEA P W KD + R ++ D+TL ++ D YTC A++ G+A SA L V
Sbjct: 68 PRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKG-----GERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYICVARNYLG--EAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGS-----PLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGE--RESKVADVTVLERPSFVKR--PSNLAVTVDDSAEFKCEARGDPVPTFGWRKDD---GELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTV 347
BLAST of roundabout 1 vs. SwissProt
Match: gi|49036492|sp|Q96MS0.2|ROBO3_HUMAN (RecName: Full=Roundabout homolog 3; AltName: Full=Roundabout-like protein 3; Flags: Precursor) HSP 1 Score: 286.574 bits (732), Expect = 2.774e-79 Identity = 253/892 (28.36%), Postives = 385/892 (43.16%), Query Frame = 0
Query: 127 EPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDG-RILELELDPSICWVQKIQGNSPSEEQKEKEKLF--------EARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDF-----------------DC--PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSN---QGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ-TSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKA-GEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQML--NLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTE-ALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLA 980
+P D+ +G A C +G P P W K+G R+ + DP + G LF AR DE + +Y +R L VA R+DF +C P+G ++ + RL K E R ++I G G L++S D G Y CVA N+AG R SA A + + P L+ P N +A V F C VKG P P +RW + +G LP R L + ++ EDE YTC+AEN G EAS L V PP + +P DQ G +V C GNP P +FW KEG +L P + G VSP G L I D+G Y C +VS +G+ILA + ++I+ S D PP+I GP NQT +GSS PC T PQ V W KDG + +D + K + TL+ ++ D Y+C AKS++G+A S +L++ + V D S P +PS+P + T N + + W + G + ++S+ IE ++ W + + E T+ +P T LVRA GLS PSP+S+P +T + + DP + L++ V L+E +LG R +++SW + P + V G+ + R S+ ++ + +S L L P T+ + + G + V + E+ P+ P+ V + + ++ + W P +G + +++ N + LN + + S +L L GL Y VAA GVG PV +++ + P+ PG+ EV + V V+ FLA
Sbjct: 69 QPPDLLVSRGEPATLPCRA-EGRPRPNIEWYKNGARVATVREDPRAHRLLLPSG-----------ALFFPRIVHGRRARPDEGVYTCVARNYLGAAASR-NASLEVAVLRDDFRQSPGNVVVAVGEPAVLECVPPRGHPEPSVSWRKDGARL-KEEEGR--ITIRG-GKLMMSHTLKSDAGMYVCVASNMAGERESAAAEVMVLERPSFLRRPVNQVVLADAPVTFLCEVKGDPPPRLRWRKEDGELPTGRYEIRSDHSLWIGHVSAEDEGTYTCVAENSVGRAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPTGRFSVSPRGQLNITAVQRGDAGYYVCQAVSVAGSILAKALLEIKGASLDGLPPVILQGPANQTLVLGSSVWLPCRVTGNPQPSVRWKKDGQWLQG-DDLQFKTMANGTLYIANVQEMDMGFYSCVAKSSTGEATWSGWLKMR-EDWGVSPDPPTEPSSPPGAPSQPVVTEITKNSITLTWKPNPQTGAA-------------------------------VTSYVIEAFSPAAGNTWRTVADGVQLETHTVSGLQPNTIYLFLVRAVGAWGLSEPSPVSEPVRTQDSSPSRPVEDPWRGQQGLAEVAV-RLQEPIVLGPRTLQVSWTVDGPVQLVQGFRVSWRVAG--PEGGSWTMLDLQSPSQQSTVLRGLPPGTQIQIKVQAQGQEGLGAESLSVTRSIPEEAPSGPPQGVAVALGGDGNSSITVSWEPPLPSQQNGVITEYQIWCLGNESRFHLNRSAAGWARSAMLRGLVPGLLYRTLVAAATSAGVGVPSAPVLVQLPSP-------PDLEPGL-----EVGAGLAVRLARVLREPAFLA 895 HSP 2 Score: 235.728 bits (600), Expect = 1.711e-62 Identity = 167/493 (33.87%), Postives = 235/493 (47.67%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKEN-DGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDG-RILELELDPSICWVQKIQGNSPSEEQKEKEKLF-------EARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQL---IRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGML---------PAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS------PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ 486
P I E P + V+R EP TL C A+G P P I+W+K+G V T D ++HR+LLPSG+LFF R V ++ D G+Y C A N G SRNASL VAVL+D FR P +V G A+ EC PP+G P P+ W+KDG R+ E E +I + + S K ++ ++ E + L P RP ++V+A F C + G+ +L G+ P R+ I + +L I V +DEG Y CVA+N G R A LS+ V P ++ P++ A G++V F C KG P P I W + + P R + QL + + D Y C A + AG + A A L++ PPV L P +Q V GS+V +PC GNP P + W K+ G L G + G L I N E D G Y+C + S +G S +K++
Sbjct: 64 PRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATVREDPRAHRLLLPSGALFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVLECVPPRGHPEPSVSWRKDGARLKEEEGRITIRGGKLMM----SHTLKSDAGMYVCVASNMAGERESAAAEVMVLER-PSFLRRPVNQVVLADAPVTFLC-----EVKGDPPPRLRWRKEDGELPTGRY--EIRSDHSLWIGHVSAEDEGTYTCVAENSVG-RAEASGSLSVHVPPQLVTQPQDQMAAPGESVAFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPTGRFSVSPRGQLNITAVQRGDAGYYVCQAVSVAGSILAKALLEIKGASLDGLPPVILQGPANQTLVLGSSVWLPCRVTGNPQPSVRWKKD---GQWLQGDDL-QFKTMANGTLYIANVQEMDMGFYSCVAKSSTGEATWSGWLKMR 539 HSP 3 Score: 73.559 bits (179), Expect = 6.796e-12 Identity = 78/297 (26.26%), Postives = 126/297 (42.42%), Query Frame = 0
Query: 303 VLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLP-------AQRVHTTDGTQLTLMNIV-----PEDEDVYTCLAENRAGFV---EASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAA-GNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAI-LASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCE--ATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
+P +++ P ++ G+ C +GRP P I W + + A R+ G L IV DE VYTC+A N G AS + V F P + G + CV G+P P + W K+G +G + + G L + +T + D+G+Y C + + +G A++EV + +RP + + PVNQ + F CE P R+ W K+ + T R ++ D++L + D YTC A+++ G+A S L V
Sbjct: 62 AMPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATVREDPRAHRLLLPSGA-LFFPRIVHGRRARPDEGVYTCVARNYLGAAASRNASLEVAVLR-DDFRQSPGNVVVAVGEPAVLECVPPRGHPEPSVSWRKDGARLK----EEEGRITIR-GGKLMMSHTLKSDAGMYVCVASNMAGERESAAAEVMV---LERPSFLRR--PVNQVVLADAPVTFLCEVKGDPPPRLRWRKEDGELPTG---RYEIRSDHSLWIGHVSAEDEGTYTCVAENSVGRAEASGSLSV 343
BLAST of roundabout 1 vs. SwissProt
Match: gi|49036501|sp|Q9Z2I4.2|ROBO3_MOUSE (RecName: Full=Roundabout homolog 3; AltName: Full=Retinoblastoma-inhibiting gene 1 protein; Short=Rig-1; Flags: Precursor) HSP 1 Score: 276.559 bits (706), Expect = 6.294e-76 Identity = 256/918 (27.89%), Postives = 393/918 (42.81%), Query Frame = 0
Query: 127 EPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDG-RILELELDPSICWVQKIQGNSPSEEQKEKEKLF--------EARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDF-----------------DC--PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSN---QGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ-TSQDRPPPIIQIGPVNQTQRIGSSTNFPCE--ATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLI--NATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKA-GEDISDPRYLRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLN--LTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTE-ALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHP--QLVPEKAPG----MDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
+P D+ +G A C +G P P W K+G R+ DP + G LF +R DE + +Y +R L VA R+DF +C PKG + + + I+L K E R ++I G G L++S D G Y CVA N+AG R S A L + P L+ P N +A V F C V+G P P + W + +G LPA R L + + EDE YTC+AEN G EAS L V PP F+ KP +Q G+ V C GNP P +FW KEG +L P + G + VSP G L I D G Y C +VS +G+ILA + ++I+ S D PPII GP NQT +GSS PC PQ ++W KD + +D + + + TLH ++ D Y+C AKS+ G+A +++LR +E+ P I T+N + + W + G + +S+ IE ++ W + + E +T+ +P T LVRA GLS PSP+S+P QT + DP + L++ V ++E +LG R +++SW + P + V G+ + R I+ LD S+ ++ + +S L L P + + + G + V + E+ P+ P+ V + + ++ + W P +G + +++ N + LN + + S+ L G Y VAA GVG PV +++ F P + PE + G + V+++ F+ S LL+GF A + +RR +Q K + HY
Sbjct: 69 QPPDLVVSRGEPATLPCRA-EGRPRPNIEWYKNGARVATAREDPRAHRLLLPSG-----------ALFFPRIVHGRRSRPDEGVYTCVARNYLGAAASR-NASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPKGHPEPLVTWKKGKIKL-KEEEGR--ITIRG-GKLMMSHTFKSDAGMYMCVASNMAGERESGAAELVVLERPSFLRRPINQVVLADAPVNFLCEVQGDPQPNLHWRKDDGELPAGRYEIRSDHSLWIDQVSSEDEGTYTCVAENSVGRAEASGSLSVHVPPQFVTKPQNQTVAPGANVSFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPMGRLLVSPRGQLNITEVKIGDGGYYVCQAVSVAGSILAKALLEIKGASIDGLPPIILQGPANQTLVLGSSVWLPCRVIGNPQPNIQWKKDERWLQG-DDSQFNLMDNGTLHIASIQEMDMGFYSCVAKSSIGEATWNSWLR---KQEDWGASPGPATGPSNPPGPPSQPIVTEVTANSITLTWKPNPQSGAT-------------------------------ATSYVIEAFSQAAGNTWRTVADGVQLETYTISGLQPNTIYLFLVRAVGAWGLSEPSPVSEPVQTQDSSLSRPAEDPWKGQRGLAEVAV-RMQEPTVLGPRTLQVSWTVDGPVQLVQGFRVSWR-IAGLDQG-SWTMLDLQSPHKQSTVLRGLPPGAQIQIKVQVQGQEGLGAESPFVTRSIPEEAPSGPPQGVAVALGGDRNSSVTVSWEPPLPSQRNGVITEYQIWCLGNESRFHLNRSAAGWARSVTFSGLLPGQIYRALVAAATSAGVGVASAPVLVQLP---FPPAAEPGPEVSEGLAERLAKVLRKPAFLAGSSAACGALLLGFCAAL-YRRQKQR-KELSHYT 926 HSP 2 Score: 230.335 bits (586), Expect = 8.433e-61 Identity = 171/493 (34.69%), Postives = 236/493 (47.87%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKEN-DGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNS--PSEEQKEKEKLF------EARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRL---GKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQ------RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS------PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ 486
P I E P + V+R EP TL C A+G P P I+W+K+G V TA D ++HR+LLPSG+LFF R V ++ D G+Y C A N G SRNASL VAVL+D FR P +V G A+ EC PPKG P P WKK G+I E + I I+G S K ++ A + E G L + P RP ++V+A +F C + G+ L G+ P R+ I + +L I QV +DEG Y CVA+N G R A LS+ V P + P+N + G V F C KG P P I W + + P+Q R+ + QL + + D Y C A + AG + A A L++ PP+ L P +Q V GS+V +PC GNP P + W K+ L G + ++ G L I + E D G Y+C + S G +S ++ Q
Sbjct: 64 PRIVEQPPDLVVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGRRSRPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPKGHPEPLVTWKK-GKIKLKEEEGRIT----IRGGKLMMSHTFKSDAGMYMCVASNMAGERESGAAELVVLERPSFLRRPINQVVLADAPVNFLC-----EVQGDPQPNLHWRKDDGELPAGRY--EIRSDHSLWIDQVSSEDEGTYTCVAENSVG-RAEASGSLSVHVPPQFVTKPQNQTVAPGANVSFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPMGRLLVSPRGQLNITEVKIGDGGYYVCQAVSVAGSILAKALLEIKGASIDGLPPIILQGPANQTLVLGSSVWLPCRVIGNPQPNIQWKKD---ERWLQGDDS-QFNLMDNGTLHIASIQEMDMGFYSCVAKSSIGEATWNSWLRKQ 539 HSP 3 Score: 76.6406 bits (187), Expect = 8.114e-13 Identity = 74/296 (25.00%), Postives = 123/296 (41.55%), Query Frame = 0
Query: 303 VLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLP-------AQRVHTTDGTQLTLMNIV-----PEDEDVYTCLAENRAGFV---EASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCV-AAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCE--ATPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRV 580
+P +++ P ++ G+ C +GRP P I W + + A R+ G L IV DE VYTC+A N G AS + V F P + G + CV G+P P++ W K G + +G + + G L + +T + D+G+Y C + + +G + + + + P P+NQ + NF CE PQ + W KD + R ++ D++L +++ D YTC A+++ G+A S L V
Sbjct: 62 AMPRIVEQPPDLVVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGA-LFFPRIVHGRRSRPDEGVYTCVARNYLGAAASRNASLEVAVLR-DDFRQSPGNVVVAVGEPAVMECVPPKGHPEPLVTWKK----GKIKLKEEEGRITIR-GGKLMMSHTFKSDAGMYMCVASNMAGERESGAAELVVLER----PSFLRRPINQVVLADAPVNFLCEVQGDPQPNLHWRKDD---GELPAGRYEIRSDHSLWIDQVSSEDEGTYTCVAENSVGRAEASGSLSV 343
BLAST of roundabout 1 vs. SwissProt
Match: gi|115558|sp|P11627.1|L1CAM_MOUSE (RecName: Full=Neural cell adhesion molecule L1; Short=N-CAM-L1; Short=NCAM-L1; AltName: CD_antigen=CD171; Flags: Precursor) HSP 1 Score: 186.422 bits (472), Expect = 6.783e-47 Identity = 236/979 (24.11%), Postives = 396/979 (40.45%), Query Frame = 0
Query: 12 FPGCWAASPPVITEH-PQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDG---EP-----VVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVV--AVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQ---GNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPK---LDTRPQIELVVACNREDFDCPKG----LEAIMGNFGRQLIRL----GKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGM----LPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQK--GVQGSTVQIPCVAAGNPVPILFWMKEGGSGVL-----LPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESG--AILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT----PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFH---LSSFQIEYYTSR-TPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVK-LSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTE---------ALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCD 931
+ G PPVITE P+ V + ++L+CEA+G P+ + +W KDG +P VV A + SGS F + S + GIY C A+N G S LV A K ++P +V + + + C PP + P +W + +I +++ D + Q N + + F + I E + L P +D +P++ L + +G LE I F I+ P R + + TL + V +D+G+Y C+A+N G A ++++ PY L+ P++ G+T R C V+GRP PEI W R NGM + + + + L L N+ P D V C A N+ G + A+A++ V P ++ +Q V+GST + C A G PVP + W+ E G+ VL P +N G L I + D+G Y C + ++ ILA+ +VK T I GP + ++ G+ F C+A+ Q + W DG + D D L + L+ SD Y+C A + E D V ++ LV+G P+ ++ + H K + ++ W P H + + IE+ P W ++ +V ++ T P V A N +G PSP+S+ TP A E +P +R + ++ M ++ + ++ + W PQ Y +Q R +++ T+S + P Y + + N + KG ++ + + ED P +PE I + N + +++W + G LKG+ V G++ + + ++ S IL L +Y V+V A+N +G+GP +
Sbjct: 26 YKGHHVLEPPVITEQSPRRLVVFPTDDISLKCEARGRPQVEFRWTKDGIHFKPKEELGVVVHEAPY--------SGS-FTIEGNNSFAQRFQGIYRCYASNKLGTAMSHEIQLVAEGAPKWPKETVKPVEVEEGE--SVVLPCNPPPSAAPPRIYWM-NSKIFDIKQDERVSMGQNGDLYFANVLTSDNHSDYICNAHFPGTRTIIQKEPIDLRVKPTNSMIDRKPRL-LFPTNSSSRLVALQGQSLILECIAEGFPTPTIKWLHPSDPMPTDRVIYQNHNK-TLQLLNVGEEDDGEYTCLAENSLGSARHA-YYVTVEAAPYWLQKPQSHLYGPGETARLDCQVQGRPQPEITW-RINGMSMETVNKDQKYRIEQGSLILSNVQPTDTMVTQCEARNQHGLLLANAYIYVVQLPARILTKDNQTYMAVEGSTAYLLCKAFGAPVPSVQWLDEEGTTVLQDERFFPYAN---------GTLSIRDLQANDTGRYFCQAANDQNNVTILANLQVKEATQ-------ITQGPRSAIEKKGARVTFTCQASFDPSLQASITWRGDGRDLQERGDSDKYFIEDGKLVIQSLDYSDQGNYSCVAST--------------------ELDEVESRAQL----------------LVVG-------SPGPVPHLELSDRHLLKQSQVHLS---WSPAEDHNSPIEKYDIEFEDKEMAPEKWFSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSESVVTPEAAPE--KNPVDVRGEGNETNNM------VITWKPLRWMDWN--APQ---IQYRVQWRPQG---KQETWRKQTVSDPFLVVSNTSTFVP---YEIKVQAVNNQGKGPEPQVTIGYSGEDYPQVSPELEDITIFNSSTVLVRWRPVDLAQVKGHLKGYNVTYWWKGSQRKHSKRHIHKSHIVVPANTTSAILSGLRPYSSYHVEVQAFNGRGLGPASE 907
BLAST of roundabout 1 vs. SwissProt
Match: gi|20455467|sp|Q05695.3|L1CAM_RAT (RecName: Full=Neural cell adhesion molecule L1; Short=N-CAM-L1; Short=NCAM-L1; AltName: Full=Nerve-growth factor-inducible large external glycoprotein; Short=NILE; AltName: CD_antigen=CD171; Flags: Precursor) HSP 1 Score: 184.496 bits (467), Expect = 2.790e-46 Identity = 234/979 (23.90%), Postives = 393/979 (40.14%), Query Frame = 0
Query: 12 FPGCWAASPPVITEH-PQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDG---EP-----VVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVV--AVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQ---GNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPK---LDTRPQIELVVACNREDFDCPKG----LEAIMGNFGRQLIRL----GKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGM----LPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQK--GVQGSTVQIPCVAAGNPVPILFWMKEGGSGVL-----LPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESG--AILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT----PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFH---LSSFQIEYYTSR-TPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLSDKRVMELKEVKILGSRKVK-LSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTE---------ALNFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCD 931
+ G PPVITE P+ V + ++L+CEA+G P+ + +W KDG +P VV A P F + S + GIY C A+N G S LV A K ++P +V + + + C PP S P + + +IL ++ D + Q N + + F + I E + L P +D +P++ L + +G LE I F I+ P R + + TL + V +D+G+Y C+A+N G A ++++ PY L+ P++ G+T R C V+GRP PE+ W R NGM + + + + L L N+ P D V C A N+ G + A+A++ V P ++ +Q V+GST + C A G PVP + W+ E G+ VL P +N G L I + D+G Y C + ++ ILA+ +VK T I GP + ++ G+ F C+A+ Q + W DG + D D L + L+ SD Y+C A + E D V ++ LV+G P+ ++ + H K + ++ W P H + + IE+ P W ++ +V ++ T P V A N +G PSP+S+ TP A E +P +R + ++ M ++ + ++ + W PQ Y +Q R L +++ T+S + P Y + + N + KG ++ + + ED P +PE I + N + +++W + G L+G+ V G++ + + ++ S IL L +Y V+V A+N +G+GP +
Sbjct: 26 YKGHHVLEPPVITEQSPRRLVVFPTDDISLKCEARGRPQVEFRWTKDGIHFKPKEELGVVVHEA---------PYSGSFTIEGNNSFAQRFQGIYRCYASNNLGTAMSHEIQLVAEGAPKWPKETVKPVEVEEGE--SVVLPCNPPP-SAAPLRIYWMNSKILHIKQDERVSMGQNGDLYFANVLTSDNHSDYICNAHFPGTRTIIQKEPIDLRVKPTNSMIDRKPRL-LFPTNSSSHLVALQGQSLILECIAEGFPTPTIKWLHPSDPMPTDRVIYQNHNK-TLQLLNVGEEDDGEYTCLAENSLGSARHA-YYVTVEAAPYWLQKPQSHLYGPGETARLDCQVQGRPQPEVTW-RINGMSIEKVNKDQKYRIEQGSLILSNVQPSDTMVTQCEARNQHGLLLANAYIYVVQLPARILTKDNQTYMAVEGSTAYLLCKAFGAPVPSVQWLDEEGTTVLQDERFFPYAN---------GHLGIRDLQANDTGRYFCQAANDQNNVTILANLQVKEATQ-------ITQGPRSTIEKKGARVTFTCQASFDPSLQASITWRGDGRDLQERGDSDKYFIEDGQLVIKSLDYSDQGDYSCVAST--------------------ELDEVESRAQL----------------LVVG-------SPGPVPHLELSDRHLLKQSQVHLS---WSPAEDHNSPIEKYDIEFEDKEMAPEKWFSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPSPVSETVVTPEAAPE--KNPVDVRGEGNETNNM------VITWKPLRWMDWN--APQ---IQYRVQWR---PLGKQETWKEQTVSDPFLVVSNTSTFVP---YEIKVQAVNNQGKGPEPQVTIGYSGEDYPQVSPELEDITIFNSSTVLVRWRPVDLAQVKGHLRGYNVTYWWKGSQRKHSKRHVHKSHMVVPANTTSAILSGLRPYSSYHVEVQAFNGRGLGPASE 907
BLAST of roundabout 1 vs. nr
Match: gi|108879336|gb|EAT43561.1| (AAEL005011-PA [Aedes aegypti]) HSP 1 Score: 530.02 bits (1364), Expect = 2.718e-166 Identity = 333/993 (33.53%), Postives = 512/993 (51.56%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQ-LTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQD-RPPPIIQIGPVNQTQRIGSSTNFPCEATPQT--RVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEF----DSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSK--LSDKRVMELKEVKILGSRKVKLSWELLIP--QKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDI-NGTEAL-NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAPGMDLV--------VQEVWF-VMVVSFFSVILLVGFLAMVCFRRIRQD 990
P ITEHP + V +N+PVTL C+A+G PEP+I+W+KDGEPV + VLLPSGSLFFLR V SKKE DGG+YWC A+N G V SRNA+L +AVL+D+FR+EP+D G TA+ ECG PKG+P +P+I W RKDEI +EL +DF R GK P AR + ++G G L+IS V+P DEG+YQC+A+N+ G R S +L++QV PY + P +++ + G V+F C V G PHP+I W + NG +P R + + L + N+ +D+ +Y C A N G + A A L V S P F++KP DQ+ C A+GNP P +FW KEG ++ P + G++H+S +G L++ ++D G + C+++S +G+ + + +++ +S D PPP++Q P N T GS PC + T ++ W K+ I+ + PR + TL ++++ DS Y+C A S G+ SAFL+V E+N+ P L P +PS P +N T++ + + W K IGG S Q + +W+ V S + +EYY+ W+ V + T+ + P+T+ +VRA N +G S PSP+S +T A + ++ P L + + + +++EL + + S V+L W+L I ++++ G +++ RD+ +S Y ++TI H +T+L T Y F+ P+ ++G+P+ +V T ED PTAAP N++ MLNLTA ++W PP +G L G+K+ + N ++ L + TL ++ S++L NLT G TY Q+ A+NR G GP P L + D H P + + + E WF V VV +IL + +V F + RQ+
Sbjct: 51 PRITEHPSDVLVPKNDPVTLNCKAEGKPEPEIRWYKDGEPV-----KFTPNHVLLPSGSLFFLRTVHSKKEQDGGVYWCVASNAAGTVNSRNATLQIAVLRDEFRVEPKDTRVAAGETALLECGAPKGNP-----------------EPTISW----------------------RKDEI-----------------MLEL------DDF--------------RPASASGKEP-ARVRI-VDG-GNLLISDVRPTDEGRYQCIAQNMVGSRESGSGKLTVQVKPYFINEPTDITVLVGQRVQFQCTVGGDPHPQILWKKENGHIPVGRAEILEEDRSLVIKNVAQDDQGIYICEAHNSVGQINAKAQLVVNSSPTFIIKPQDQRTNLNGVATFKCTASGNPPPSVFWTKEGSQTLMFPNNTYGNIHISSQGSLQVRGAQKEDDGYFVCSALSVAGSTTSRAYLQVTSSFDSSPPPVLQEVPSNLTLPRGSIAMLPCRGSGPTSPKIYWKKNATDITALG-PRFSIVQGGTLKIDDIQPEDSGWYSCVAYSEKGETSWSAFLQV---EKNLSTMLPRSGSPEL--LPVAPSIPKAVNITNSNVTLMWTK---IGGGGEDSLQEK---------------LWNNPV---SGYLVEYYSPDEKKGWIRAIRVPGNTA-TITNLSPSTSYIFVVRAENAYGYSAPSPLSNLIRT-LSADDGVTMPEELEAARMVLNGKILELVDAVAISSTSVRLEWQLHISSTEEYIEGLYVRYRDLG--ANSQKYSMLTIPNNAAEMHVITNLDKFTRYEFFLTPFFKNVEGQPSNSKVVQTAEDAPTAAPVNIQTGMLNLTAGWVKWSPPPAEHQNGVLLGYKIQVKAGNSSKTLASMTLNSTTTSVMLNNLTTGATYRAQIVAFNRVGAGPYSKPAYLIM--DPAHVIAPPRAHNSLSDIGGYRHHNFMHETWFMVFVVLALLIILTFTVVGVVVFIKKRQN 926
BLAST of roundabout 1 vs. nr
Match: gi|1218261951|ref|XP_021709627.1| (protein sax-3 isoform X2 [Aedes aegypti]) HSP 1 Score: 530.02 bits (1364), Expect = 8.775e-165 Identity = 333/993 (33.53%), Postives = 512/993 (51.56%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQ-LTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQD-RPPPIIQIGPVNQTQRIGSSTNFPCEATPQT--RVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEF----DSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSK--LSDKRVMELKEVKILGSRKVKLSWELLIP--QKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDI-NGTEAL-NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAPGMDLV--------VQEVWF-VMVVSFFSVILLVGFLAMVCFRRIRQD 990
P ITEHP + V +N+PVTL C+A+G PEP+I+W+KDGEPV + VLLPSGSLFFLR V SKKE DGG+YWC A+N G V SRNA+L +AVL+D+FR+EP+D G TA+ ECG PKG+P +P+I W RKDEI +EL +DF R GK P AR + ++G G L+IS V+P DEG+YQC+A+N+ G R S +L++QV PY + P +++ + G V+F C V G PHP+I W + NG +P R + + L + N+ +D+ +Y C A N G + A A L V S P F++KP DQ+ C A+GNP P +FW KEG ++ P + G++H+S +G L++ ++D G + C+++S +G+ + + +++ +S D PPP++Q P N T GS PC + T ++ W K+ I+ + PR + TL ++++ DS Y+C A S G+ SAFL+V E+N+ P L P +PS P +N T++ + + W K IGG S Q + +W+ V S + +EYY+ W+ V + T+ + P+T+ +VRA N +G S PSP+S +T A + ++ P L + + + +++EL + + S V+L W+L I ++++ G +++ RD+ +S Y ++TI H +T+L T Y F+ P+ ++G+P+ +V T ED PTAAP N++ MLNLTA ++W PP +G L G+K+ + N ++ L + TL ++ S++L NLT G TY Q+ A+NR G GP P L + D H P + + + E WF V VV +IL + +V F + RQ+
Sbjct: 26 PRITEHPSDVLVPKNDPVTLNCKAEGKPEPEIRWYKDGEPV-----KFTPNHVLLPSGSLFFLRTVHSKKEQDGGVYWCVASNAAGTVNSRNATLQIAVLRDEFRVEPKDTRVAAGETALLECGAPKGNP-----------------EPTISW----------------------RKDEI-----------------MLEL------DDF--------------RPASASGKEP-ARVRI-VDG-GNLLISDVRPTDEGRYQCIAQNMVGSRESGSGKLTVQVKPYFINEPTDITVLVGQRVQFQCTVGGDPHPQILWKKENGHIPVGRAEILEEDRSLVIKNVAQDDQGIYICEAHNSVGQINAKAQLVVNSSPTFIIKPQDQRTNLNGVATFKCTASGNPPPSVFWTKEGSQTLMFPNNTYGNIHISSQGSLQVRGAQKEDDGYFVCSALSVAGSTTSRAYLQVTSSFDSSPPPVLQEVPSNLTLPRGSIAMLPCRGSGPTSPKIYWKKNATDITALG-PRFSIVQGGTLKIDDIQPEDSGWYSCVAYSEKGETSWSAFLQV---EKNLSTMLPRSGSPEL--LPVAPSIPKAVNITNSNVTLMWTK---IGGGGEDSLQEK---------------LWNNPV---SGYLVEYYSPDEKKGWIRAIRVPGNTA-TITNLSPSTSYIFVVRAENAYGYSAPSPLSNLIRT-LSADDGVTMPEELEAARMVLNGKILELVDAVAISSTSVRLEWQLHISSTEEYIEGLYVRYRDLG--ANSQKYSMLTIPNNAAEMHVITNLDKFTRYEFFLTPFFKNVEGQPSNSKVVQTAEDAPTAAPVNIQTGMLNLTAGWVKWSPPPAEHQNGVLLGYKIQVKAGNSSKTLASMTLNSTTTSVMLNNLTTGATYRAQIVAFNRVGAGPYSKPAYLIM--DPAHVIAPPRAHNSLSDIGGYRHHNFMHETWFMVFVVLALLIILTFTVVGVVVFIKKRQN 901
BLAST of roundabout 1 vs. nr
Match: gi|1218261949|ref|XP_021709626.1| (protein sax-3 isoform X1 [Aedes aegypti]) HSP 1 Score: 529.25 bits (1362), Expect = 7.572e-164 Identity = 333/993 (33.53%), Postives = 512/993 (51.56%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQ-LTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQD-RPPPIIQIGPVNQTQRIGSSTNFPCEATPQT--RVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEF----DSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSK--LSDKRVMELKEVKILGSRKVKLSWELLIP--QKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDI-NGTEAL-NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAPGMDLV--------VQEVWF-VMVVSFFSVILLVGFLAMVCFRRIRQD 990
P ITEHP + V +N+PVTL C+A+G PEP+I+W+KDGEPV + VLLPSGSLFFLR V SKKE DGG+YWC A+N G V SRNA+L +AVL+D+FR+EP+D G TA+ ECG PKG+P +P+I W RKDEI +EL +DF R GK P AR + ++G G L+IS V+P DEG+YQC+A+N+ G R S +L++QV PY + P +++ + G V+F C V G PHP+I W + NG +P R + + L + N+ +D+ +Y C A N G + A A L V S P F++KP DQ+ C A+GNP P +FW KEG ++ P + G++H+S +G L++ ++D G + C+++S +G+ + + +++ +S D PPP++Q P N T GS PC + T ++ W K+ I+ + PR + TL ++++ DS Y+C A S G+ SAFL+V E+N+ P L P +PS P +N T++ + + W K IGG S Q + +W+ V S + +EYY+ W+ V + T+ + P+T+ +VRA N +G S PSP+S +T A + ++ P L + + + +++EL + + S V+L W+L I ++++ G +++ RD+ +S Y ++TI H +T+L T Y F+ P+ ++G+P+ +V T ED PTAAP N++ MLNLTA ++W PP +G L G+K+ + N ++ L + TL ++ S++L NLT G TY Q+ A+NR G GP P L + D H P + + + E WF V VV +IL + +V F + RQ+
Sbjct: 26 PRITEHPSDVLVPKNDPVTLNCKAEGKPEPEIRWYKDGEPV-----KFTPNHVLLPSGSLFFLRTVHSKKEQDGGVYWCVASNAAGTVNSRNATLQIAVLRDEFRVEPKDTRVAAGETALLECGAPKGNP-----------------EPTISW----------------------RKDEI-----------------MLEL------DDF--------------RPASASGKEP-ARVRI-VDG-GNLLISDVRPTDEGRYQCIAQNMVGSRESGSGKLTVQVKPYFINEPTDITVLVGQRVQFQCTVGGDPHPQILWKKENGHIPVGRAEILEEDRSLVIKNVAQDDQGIYICEAHNSVGQINAKAQLVVNSSPTFIIKPQDQRTNLNGVATFKCTASGNPPPSVFWTKEGSQTLMFPNNTYGNIHISSQGSLQVRGAQKEDDGYFVCSALSVAGSTTSRAYLQVTSSFDSSPPPVLQEVPSNLTLPRGSIAMLPCRGSGPTSPKIYWKKNATDITALG-PRFSIVQGGTLKIDDIQPEDSGWYSCVAYSEKGETSWSAFLQV---EKNLSTMLPRSGSPEL--LPVAPSIPKAVNITNSNVTLMWTK---IGGGGEDSLQEK---------------LWNNPV---SGYLVEYYSPDEKKGWIRAIRVPGNTA-TITNLSPSTSYIFVVRAENAYGYSAPSPLSNLIRT-LSADDGVTMPEELEAARMVLNGKILELVDAVAISSTSVRLEWQLHISSTEEYIEGLYVRYRDLG--ANSQKYSMLTIPNNAAEMHVITNLDKFTRYEFFLTPFFKNVEGQPSNSKVVQTAEDAPTAAPVNIQTGMLNLTAGWVKWSPPPAEHQNGVLLGYKIQVKAGNSSKTLASMTLNSTTTSVMLNNLTTGATYRAQIVAFNRVGAGPYSKPAYLIM--DPAHVIAPPRAHNSLSDIGGYRHHNFMHETWFMVFVVLALLIILTFTVVGVVVFIKKRQN 901
BLAST of roundabout 1 vs. nr
Match: gi|752883644|ref|XP_011259531.1| (PREDICTED: protein sax-3 [Camponotus floridanus] >gi|307176930|gb|EFN66247.1| Roundabout-like protein 2 [Camponotus floridanus]) HSP 1 Score: 451.055 bits (1159), Expect = 6.837e-136 Identity = 266/752 (35.37%), Postives = 395/752 (52.53%), Query Frame = 0
Query: 255 EARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG-SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS-DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
EA +++ G L+IS V+P D+G+YQC+A+N+ GI+ SA A L++ V PY + P N + +A T F C V G P PEI W R +G +P R H + L + + P+D+ Y C AEN G + ASA L V S PVF P D+ S V C A G P P +FW +EG ++ PG + Q H V+ G L I KD G Y C+++S++GA A+ +++ + ++ PPPII++GP NQT + S + PC A TP RV W K+GV + RI + + TL ++L +DS LYTC A S SG SA+L V+ F P +S P +PS+P ++NATSN + + W P G S I Y IEYY+S T WV + +T+ D P T LVRA N HGLS P P+S QT + R++ + ++ LKEV+ L S VK+ W++L V G +I+ R++S+ Y ++T+ G S+ LT+L+ T Y F++P+ I+GRP+ + +VTT EDVP+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +LT G Y +VA R G+GP P L ++ QL P P G V++E WF++++ I++V LA + RR + K +GH N
Sbjct: 54 EATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGIKGSAIATLTVHVKPYFVATPSNQTILADQTAEFVCRVGGDPSPEILWRRGDGKMPIGRAHILEDKSLHIERVNPQDQGTYICEAENGVGTISASATLTVHSRPVFSNFPKDETVSSNSDVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQNHYTVTENGSLTIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGG--RITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTVSSG---ASFHRTPDVSALPQNPSKPRIVNATSNSVTLTW-SPGHEGQSKIIGYN------------------------------IEYYSSNLNTGWVVAATGVLDDIYTVTDLRPETNYVFLVRAENNHGLSLPGPLSDVVQTTNVNQHTVPQVELTRARDRLNSEILHLKEVQPLTSTSVKIMWDILGAADLVEGLYIRYREVSE---KPEYQMVTVLNAGATSYVLTNLKKYTRYEFFLVPFYKIIEGRPSNVKLVTTLEDVPSGAPENVHVGMINMTSAFVRWSPPPKNTQNGQLIGYKIQIKSNSSNKILGQMSLNASTTSVIINSLTAGGLYTARVAGQTRIGLGPFSSPTLLNMDPGQI-TQLPPRTDPSHGGASVIRETWFLVLMIMMIFIVIVVLLAALYVRRRQAMSKQLGHLN 765 HSP 2 Score: 178.333 bits (451), Expect = 8.956e-42 Identity = 128/395 (32.41%), Postives = 190/395 (48.10%), Query Frame = 0
Query: 115 LVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQ----KIQGNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLE---AIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHT----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT 487
L VAVL+++FR EPQ+ G TA+ ECGPP+G P PT +WK++G I++LE I V I P+++ K + E + + I T LT+ P P + ++A +F C G + I+ G GK P R I + +L I +V P+D+G Y C A+N G +SA A L++ P P++ + + V F CA +G P P I W+R M P T+ LT+ V +DE Y C A ++AG A+ LQV S PP+ + P +Q S +PC A G P P + W KEG ++ PG G + ++ G L I + DSG+YTC + SESG S+ + + +
Sbjct: 3 LSVAVLREEFRAEPQNTRVAAGETALLECGPPRGHPEPTLYWKRNGHIIDLEATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGIKGSAIAT--LTVHVKPYFVATPSNQTILADQTAEFVCRVGGDPSPEILWRRGD-----GKMPIGR--AHILEDKSLHIERVNPQDQGTYICEAENGVGT-ISASATLTVHSRPVFSNFPKDETVSSNSDVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQNHYTVTENGSLTIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEG--VLVRPG---GRITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTVSS 382 HSP 3 Score: 85.8853 bits (211), Expect = 3.587e-13 Identity = 104/397 (26.20%), Postives = 163/397 (41.06%), Query Frame = 0
Query: 27 PQNSSVTRNEPVTLRCEA-QGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKK-DGRI----LELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGT----ESLTLSYCPKLDTRPQIELVVACNREDFDC-----PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVL-------PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGML-PAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKP 400
PQN+ V E L C +G PEP + W ++G + A + R+ L G + V + D G Y C A N G S A+L V V K F P + + TA F C G P+P W++ DG++ + D S+ I+ +P Q + + EA ++ +GT +LT+ P P+ E V + + F C PK ++L+ G + + ++ G+L I KDEG Y C A + AG ++ A + LQV P + P N + C G P P + W + ++ P R+ L + ++ D +YTC+A + +G SA+L V S F P
Sbjct: 16 PQNTRVAAGETALLECGPPRGHPEPTLYWKRNGHIIDLEA----TKRITLVDGGNLMISDV---RPADQGKYQCIAENMVGIKGSAIATLTVHV-KPYFVATPSNQTILADQTAEFVC-RVGGDPSPEILWRRGDGKMPIGRAHILEDKSL----HIERVNP---QDQGTYICEA-ENGVGTISASATLTVHSRPVFSNFPKDETVSSNSDVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQNHY--TVTENGSLTIKGAVRKDEGHYVCSAISQAG---ASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGGRITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTVSSGASFHRTP 390
BLAST of roundabout 1 vs. nr
Match: gi|1233169468|ref|XP_022186783.1| (roundabout homolog 2-like [Nilaparvata lugens]) HSP 1 Score: 449.514 bits (1155), Expect = 1.389e-135 Identity = 272/800 (34.00%), Postives = 412/800 (51.50%), Query Frame = 0
Query: 221 LVVACNREDFDC-PKGLEAIMGNFGRQLIRLG---KRPEARFLLSIEGE---------------GTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKA-GEDISDPRYL---RSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL-NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAPGMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHM 994
L VA REDF PK G L+ G PE +G+ G L++++V+ DEG+YQC+ +N+ G+R S A L++ V PY +K P +V+ +A V F C V G P P+I W R +G +P R H D L + ++ PEDE +Y C AEN G V A A L V SPPVF+ KP DQK CVA GNP P +FW KEG ++ PG++ GH+HV+ EG LRI +D+G C+++S +G+ + +++ + D PPPI++IGP NQT I S N PC+A TP ++W KD P+ + +PR+ + TL +L++ DS LYTC A S SG+ SA L V A P S FPS P P ++N T + + I WD +P+ Y V EY++S T WV ++ T+ D +P T+ +VRA N HGLS PS SK +T + G P+ L R +L K V+ L+++ + S VKL WE+L +KFV G +++ RD+S S Y+++T+ G S+T+ +LR T+Y F++P+ ++G+P+ +V T EDVP+A P+N+++ +N TA + W PP + +G + G+K+ I NGT+ + ++ S+ S++L NL V+VAA R G+GP V + + P P P + W V++++F +VILL+ ++ + R+ KH+
Sbjct: 113 LQVAVLREDFRAEPKDTRVAAGETA--LLECGPPKGHPEPTLFWKKDGQVLDLETSNRVRVVDGGNLMMTEVRQADEGRYQCLVQNLVGMRESGHATLTVHVKPYFVKEPSDVTVIADQMVDFECRVGGDPSPKILWRRDDGKMPIGRAHLLDDKSLRIEHVTPEDEGLYICDAENLVGSVSARASLTVHSPPVFITKPQDQKVGLNGIASFDCVAKGNPPPSVFWSKEGSQVLMFPGNSYGHLHVTAEGSLRIQGAQREDAGFLVCSALSVAGSTTVRAFLQVTSVDDVPPPIVEIGPSNQTLPIDSVANLPCQARGTPHPIIKWYKDSQPLDSTVNPRVNILTSGTLQINDLQLVDSGLYTCTASSESGETSWSASLSVEKA-PGTHLHRSPDPSTFPSPPGTPQVLNITQSNITITWDP--GENAAPLIGYTV------------------------------EYFSSDLQTGWVVAAHRINNRSITIGDLKPETSYVFVVRAENAHGLSVPSDTSKVVRTLGSSEGTRTVPPQLLDEARVRLGTK-VIVLQDLSPVSSTSVKLVWEILNAEKFVEGLYVRFRDLS--GGSQKYNMVTVLNAGATSYTVVNLRKFTKYEFFLVPFFKTVEGQPSNSKIVQTLEDVPSAPPDNIQVGSINSTAVFVHWSPPPPQHYNGIILGYKLQIKSNGTKVVAQMSMNVSTTSILLNNLASNGMLTVRVAALTRVGIGPYS--VSMPLVFSRLGPYSQPTTGP------TQTWLVLILAFLAVILLLATVSTLYVRKRHGLTKHI 866 HSP 2 Score: 276.559 bits (706), Expect = 1.372e-73 Identity = 181/500 (36.20%), Postives = 254/500 (50.80%), Query Frame = 0
Query: 7 NVLFSFPGCWAASPPVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSH--RVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE-------LDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQ---RVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQ 486
+V+ + G + + P ITEHP ++ V +NEPVTL C+A+G PEP I+W+KDG+ V +A + VLLP+GSLFFLRA KKE+D G+YWC A N G SRNA+L VAVL++ FR EP+D G TA+ ECGPPKG P PT FWKKDG++L+LE +D + +++ Q + L R E G +LT+ P P V+A DF+C G + R+ GK P R L + +L I V P+DEG Y C A+N+ G +SA+A L++ P + P++ F C KG P P + WS+ M P +H T L + ED C A + AG A LQV S PP+ + P++Q S +PC A G P PI+ W K+ L + V++ G L+I + DSG+YTC + SESG S+ + ++
Sbjct: 5 SVILNLSGQYRS--PRITEHPASTVVPKNEPVTLNCKAEGRPEPDIEWWKDGQRVEVSANTQRDSIGPVLLPAGSLFFLRARHGKKEHDDGVYWCVARNLAGSATSRNATLQVAVLREDFRAEPKDTRVAAGETALLECGPPKGHPEPTLFWKKDGQVLDLETSNRVRVVDGGNLMMTEVRQADEGRYQCLVQNLVGMR--ESGHATLTVHVKPYFVKEPSDVTVIADQMVDFECRVGGDPSPKILWRR--DDGKMPIGRAHL--LDDKSLRIEHVTPEDEGLYICDAENLVG-SVSARASLTVHSPPVFITKPQDQKVGLNGIASFDCVAKGNPPPSVFWSKEGSQVLMFPGNSYGHLHVTAEGSLRIQGAQREDAGFLVCSALSVAGSTTVRAFLQVTSVDDVPPPIVEIGPSNQTLPIDSVANLPCQARGTPHPIIKWYKDSQP---LDSTVNPRVNILTSGTLQINDLQLVDSGLYTCTASSESGETSWSASLSVE 492
BLAST of roundabout 1 vs. nr
Match: gi|815815103|ref|XP_012228596.1| (PREDICTED: roundabout homolog 2-like [Linepithema humile]) HSP 1 Score: 452.21 bits (1162), Expect = 1.901e-135 Identity = 293/901 (32.52%), Postives = 444/901 (49.28%), Query Frame = 0
Query: 127 EPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE---------LDP--SICWVQKIQGNSPSEEQKEKEKLFEARKD--EIGTESLTLSYCPKLD---TRPQIELVVACNREDFDC--PKGLEAIMGNFGR--QLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG-SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEAT--PQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRY--LRSKLSDKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
P D+ + C +G P P W KDG +++ L P S+ +++ + G +EQ AR + + + TL+ D PQ V A +C P+G + R +I L EA +++ L+IS V+P D+G+YQC+A+N+ GI+ SA A L++ V P+ L P N + + T F C V G P PEI W R +G +P R D L + + PED+ Y C A+N G + A+A L V S P+F P D+ G+ V C A G P P +FW +EG ++ PG + QGH V+ G L I +KD G Y C+++S++GA + +++ + ++ PPPII++GP NQT + S PC A P RV W K+GV + RI + + TL ++L +DS LYTC A S SG SA+L V+ F P +S P +PS+P ++NATSN + + W P G S I Y IEYY+S T WV + +T+ D P T LVRA N HGLS P P+S QT +S +R +L + ++ LKEV+ L S VK+ W++L V G +I+ R++S+ Y ++T+ G S+ LT+L+ Y F++P+ I+GRP+ + +VTT EDVP+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +LT G Y +VA R G+GP P L ++ PQL P P G VV+E WF++++ +V ++ L + RR + K +GH N
Sbjct: 35 HPSDITVAKNEPVTLNC-KAEGKPEPVIEWYKDGELVQTSPGDAKSHRVLLPTGSLFFLRVMHGK---KEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLQWKRNGHIIDL----EATKRITLVDGSNLMISDVRPADQGKYQCIAENLVGIKESAIATLTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPPPEILWRRHDGKMPIGRGRILDDKSLRIERVNPEDQGTYICEADNGIGTISANATLTVHSKPIFTNFPKDETVNAGTNVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQGHYTVTEGGSLSIKGAVKKDEGYYVCSAISQAGASTVTVFLQVTSVEEIPPPIIELGPANQTLPLKSEAWLPCRAVGIPTPRVHWHKEGVLVQPGG--RITMAINGTLFIDDLRANDSGLYTCIASSESGNTSWSAYLTVSSG---ASFHRTPDISALPQNPSKPRIVNATSNSITLTW-SPGDEGQSKIIGYN------------------------------IEYYSSNLNTGWVVAATGVVDDIYTVPDLRPETNYVFLVRAENSHGLSLPGPLSDVVQTTNVNQHTVSQVELTRIRDRL-NSEILHLKEVQPLSSTSVKIMWDILGAADLVEGLYIRYREVSE---KPEYQMVTVLNAGATSYVLTNLKKFMRYEFFLVPFYKTIEGRPSNVKLVTTLEDVPSGAPENVHVGMINMTSAFVRWSPPPKNTQNGQLIGYKIQIKTNSSNKILGQMSLNASTTSVIINSLTTGGLYTARVAGQTRVGLGPFSSPTLLNMDPTQLTPQLPPRTDPSHGGASVVRETWFLVLMITMTVTVIAALLTALYVRRRQAMSKQLGHLN 887 HSP 2 Score: 306.605 bits (784), Expect = 1.858e-83 Identity = 195/497 (39.24%), Postives = 265/497 (53.32%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQ----KIQGNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVH----TTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLL-PGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT--SQDRPPPI 495
P ITEHP + +V +NEPVTL C+A+G PEP I+W+KDGE V T+ D KSHRVLLP+GSLFFLR + KKE DGG+YWC A N G V SRNA+L VAVL+D+FR EPQ+ G TA+ ECGPP+G P PT WK++G I++LE I V I P+++ K + E L ++ I T LT+ P P + ++ +F C G + R+ GK P R I + +L I +V P+D+G Y C A N G +SA A L++ P P++ + AG V F CA +G P P I W+R M P T+G L++ V +DE Y C A ++AG + LQV S PP+ + P +Q S +PC A G P P + W KE GVL+ PG G + ++ G L I + DSG+YTC + SESG S+ + + + S R P I
Sbjct: 30 PRITEHPSDITVAKNEPVTLNCKAEGKPEPVIEWYKDGELVQTSPGDAKSHRVLLPTGSLFFLRVMHGKKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLQWKRNGHIIDLEATKRITLVDGSNLMISDVRPADQGKYQCIAENLVGIKESAIAT--LTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPPPEILWRR--HDGKMPIGRG--RILDDKSLRIERVNPEDQGTYICEADNGIGT-ISANATLTVHSKPIFTNFPKDETVNAGTNVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQGHYTVTEGGSLSIKGAVKKDEGYYVCSAISQAGASTVTVFLQVTSVEEIPPPIIELGPANQTLPLKSEAWLPCRAVGIPTPRVHWHKE---GVLVQPG---GRITMAINGTLFIDDLRANDSGLYTCIASSESGNTSWSAYLTVSSGASFHRTPDI 513
BLAST of roundabout 1 vs. nr
Match: gi|826448266|ref|XP_012531725.1| (PREDICTED: roundabout homolog 2-like [Monomorium pharaonis]) HSP 1 Score: 451.055 bits (1159), Expect = 5.331e-135 Identity = 293/900 (32.56%), Postives = 447/900 (49.67%), Query Frame = 0
Query: 127 EPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE---------LDP--SICWVQKIQGNSPSEEQKEKEKLFEARKD--EIGTESLTLSYCPKLD---TRPQIELVVACNREDFDC--PKGLEAIMGNFGR--QLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG-SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS-DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
P D+ + C +G P P W KDG +++ L P S+ +++ + G +EQ AR + + + TL+ D PQ V A +C P+G ++ R +I L EA +++ G L+IS V+P D+G+YQC+A+N+ G + SA A L++ V P+ L P N + + T F C V G P PEI W R +G +P R H + L + + P+D+ Y C AEN G + ASA L V S PVF P D+ G+ V C A G P P +FW +EG ++ PG + Q H +V+ +G L I KD G Y C+++S++GA A+ +++ + ++ PPPII++GP NQT + S + PC A TP RV+W K+GV + + RI + + TL ++L +DS LYTC A S SG SA+L V+ F P +S P +PS+P ++NATSN + + W P G S I Y IEYY+S T WV + +T+ D P T LVRA N GLS P P+S QT + R++ + ++ LKEV+ L S VK+ W++L V G +I+ R+ S+ Y ++T+ G S+ LT+L+ T Y F++P+ I+GRP+ + +VTT EDVP+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +LT G Y +VA R G+GP +P L ++ QL P P G VV+E WF++++ ++ LA + RR + K +GH N
Sbjct: 33 HPSDIIVAKNEPVTLNC-KAEGKPEPVIEWYKDGELVQTSPGDAKSHRVLLPTGSLFFLRVMHG---KKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDL----EATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAIATLTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPEPEILWRRSDGKMPIGRAHILNDKSLHIERVNPQDQGTYICDAENSVGAISASATLTVHSRPVFTNFPKDETVNAGADVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQTHYNVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSIEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVQWHKEGVLVQSGG--RITMAINGTLFIDDLHAADSGLYTCIASSESGNTSWSAYLTVS--STGASFHRTPDVSALPQNPSKPRIVNATSNSVTLTW-SPGHEGQSKIIGYN------------------------------IEYYSSNLNTGWVVAATGVLDDIYTVTDLRPETNYVFLVRAENSQGLSLPGPLSDLVQTTNVNQHTVPQVELTRARDRLNSEILHLKEVQPLTSTSVKIMWDILGAADLVEGLYIRYREDSE---KPEYQMVTVLNAGATSYVLTNLKKYTRYEFFLVPFYKTIEGRPSNVKLVTTLEDVPSGAPENVHVGMINMTSAFVRWSPPPKNTQNGQLIGYKIQIKSNSSNKILGQMSLNASTTSVIINSLTTGGLYTARVAGQTRVGLGPFSNPTLLNMDPGQL-TQLPPRTDPSHGGASVVRETWFLVLIITMIFTVIAALLAALYVRRRQAMSKQLGHLN 885 HSP 2 Score: 306.99 bits (785), Expect = 1.080e-83 Identity = 189/487 (38.81%), Postives = 262/487 (53.80%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQ----KIQGNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHT----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTS 488
P ITEHP + V +NEPVTL C+A+G PEP I+W+KDGE V T+ D KSHRVLLP+GSLFFLR + KKE DGG+YWC A N G V SRNA+L VAVL+D+FR EPQ+ G TA+ ECGPP+G P PT WK++G I++LE I V I P+++ K + E + ++ I T LT+ P P + ++ +F C G + R+ GK P R I + +L I +V P+D+G Y C A+N G +SA A L++ P P++ + AG V F CA +G P P I W+R M P T T+ L++ V +DE Y C A ++AG A+ LQV S PP+ + P +Q S +PC A G P P + W KE GVL+ + G + ++ G L I + DSG+YTC + SESG S+ + + ++
Sbjct: 28 PRITEHPSDIIVAKNEPVTLNCKAEGKPEPVIEWYKDGELVQTSPGDAKSHRVLLPTGSLFFLRVMHGKKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDLEATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAIAT--LTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPEPEILWRR--SDGKMPIGR--AHILNDKSLHIERVNPQDQGTYICDAENSVGA-ISASATLTVHSRPVFTNFPKDETVNAGADVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQTHYNVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSIEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVQWHKE---GVLV--QSGGRITMAINGTLFIDDLHAADSGLYTCIASSESGNTSWSAYLTVSST 502
BLAST of roundabout 1 vs. nr
Match: gi|795043141|ref|XP_011867498.1| (PREDICTED: roundabout homolog 2-like [Vollenhovia emeryi]) HSP 1 Score: 450.284 bits (1157), Expect = 1.226e-134 Identity = 262/752 (34.84%), Postives = 394/752 (52.39%), Query Frame = 0
Query: 255 EARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG-SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS-DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
EA +++ G L+IS V+P D+G+YQC+A+N+ G + SA A L++ V P+ L P N + + T F C V G P PEI W R +G +P R H + L + + P+D+ Y C AEN G + ASA L V S P+F P D+ G+ V C A G P P +FW +EG ++ PG + Q H V+ +G L I KD G Y C+++S++GA A+ +++ + ++ PPPII++GP NQT + S + PC A TP RV W K+GV + RI + + TL ++L +DS LYTC A S SG SA+L V+ F P +S P +PS+P ++NATSN + + W P G S I Y IEYY+S T WV + +T+ D P T LVRA N GLS P P+S QT + R++ + ++ LKEV+ L S VK+ W++L V G +I+ R++S+ Y ++T+ G S+ LT+L+ T Y F++P+ I+GRP+ + +VTT EDVP+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +LT G Y +VA R G+GP P L ++ QL P P G VV+E WF++++ + ++V LA + RR + K +GH N
Sbjct: 173 EATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAIAVLTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPEPEILWRRNDGKMPIGRAHILNDKSLHIERVNPQDQGTYICDAENGVGAISASATLTVHSRPIFTNFPKDETVNAGTDVTFSCAARGAPNPSIFWTREGSQELMFPGHTYQSHYTVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGG--RITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTVSTG---ASFHRTPDVSALPQNPSKPRIVNATSNSVTLTW-SPGHEGQSKIVGYN------------------------------IEYYSSNLNTGWVVAATGVVDDIYTVTDLRPETNYVFLVRAENSQGLSLPGPLSDVVQTTNVNQHTVPQVELTRARDRLNSEILHLKEVQPLTSTSVKIMWDILGAADLVEGLYIRYREVSE---KPEYQMVTVLNAGATSYVLTNLKKYTRYEFFLVPFYKIIEGRPSNVKLVTTLEDVPSGAPENVHVGMINMTSAFVRWSPPPKNTQNGQLVGYKIQIKSNSSNKILGQMSLNASTTSVIINSLTTGGLYTARVAGQTRVGLGPFSSPTLLNMDPGQL-TQLPPRTDPSHGGASVVRETWFLVLIITMILTVIVALLAALYVRRRQAMSKQLGHLN 884 HSP 2 Score: 307.375 bits (786), Expect = 9.479e-84 Identity = 190/487 (39.01%), Postives = 262/487 (53.80%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQK----IQGNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHT----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLL-PGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT 487
P ITEHP + V +NEPVTL C+A+G PEP I+W+KDGE V T+ D KSHRVLLP+GSLFFLR + KKE DGG+YWC A N G V SRNA+L VAVL+D+FR EPQ+ G TA+ ECGPP+G P PT WK++G I++LE I V I P+++ K + E + ++ I LT+ P P + ++ +F C G + R+ GK P R I + +L I +V P+D+G Y C A+N G +SA A L++ P P++ + AG V F CA +G P+P I W+R M P + T+ L++ V +DE Y C A ++AG A+ LQV S PP+ + P +Q S +PC A G P P + W KE GVL+ PG G + ++ G L I + DSG+YTC + SESG S+ + + T
Sbjct: 28 PRITEHPSDIIVAKNEPVTLNCKAEGKPEPLIEWYKDGELVQTSPGDAKSHRVLLPTGSLFFLRVMHGKKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDLEATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAIAV--LTVHVKPFFLAMPSNQTILTDQTAEFACRVGGDPEPEILWRR--NDGKMPIGR--AHILNDKSLHIERVNPQDQGTYICDAENGVGA-ISASATLTVHSRPIFTNFPKDETVNAGTDVTFSCAARGAPNPSIFWTREGSQELMFPGHTYQSHYTVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKE---GVLVRPG---GRITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTVST 501
BLAST of roundabout 1 vs. nr
Match: gi|1070595524|ref|XP_018394917.1| (PREDICTED: roundabout homolog 2-like [Cyphomyrmex costatus]) HSP 1 Score: 449.899 bits (1156), Expect = 1.479e-134 Identity = 293/900 (32.56%), Postives = 445/900 (49.44%), Query Frame = 0
Query: 127 EPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE---------LDP--SICWVQKIQGNSPSEEQKEKEKLFEARKD--EIGTESLTLSYCPKLD---TRPQIELVVACNREDFDC--PKGLEAIMGNFGR--QLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPG-SNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS-DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWFVMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
P D+ + C +G P P W KDG +++ L P S+ +++ + G +EQ AR + + + TL+ D PQ V A +C P+G ++ R +I L EA +++ G L+IS V+P D+G+YQC+A+N+ G + SA A L++ V PY L P N + +A T F C V G P PEI W R +G +P R H + L + + P+D+ Y C AEN G + ASA L V S PVF P D+ G+ V C A G P P +FW +EG ++ PG + Q H V+ +G L I KD G Y C+++S++GA A+ +++ + ++ PPPII++GP NQT + S + PC A TP RV W K+GV + RI + + TL ++L +DS LYTC A S SG SA+L ++ F P +S P +PS+P ++NATSN + + W P G S I Y IEYY+S T WV + +T+ D P T LVRA N GLS P P+S QT + R++ + ++ LKEV+ L S VK+ W++L V G +I+ R++S+ Y ++T+ G S+ LT+L+ T Y F++P+ I+GRP+ + +VTT ED+P+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +LT G Y +VA R G+GP P L ++ QL P P G VV+E WF++++ ++ LA + RR + K +GH N
Sbjct: 33 HPSDIIVAKNEPVTLNC-KAEGKPEPVIEWYKDGELVQTSPGDTKSHRVLLPTGSLFFLRVMHG---KKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDL----EATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAVAVLTVHVKPYFLAMPSNQTILADQTAEFACRVGGDPEPEILWRRNDGKMPIGRAHILNDKSLHIERVNPQDQGTYICDAENGVGAISASATLTVHSRPVFTNFPKDETVNAGTDVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQSHYTVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGG--RITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTISTG---ASFHRTPDMSALPQNPSKPRIVNATSNSVTLTW-SPGHEGQSKIIGYN------------------------------IEYYSSNLNTGWVVAATGVLDDIYTVTDLRPETNYVFLVRAENSQGLSLPGPLSDVVQTTNANQHTVPQVELTRARDRLNSEILHLKEVQPLTSTSVKIMWDILGAADLVEGLYIRYREVSE---KPEYQMVTVLNAGATSYVLTNLKKYTRYEFFLVPFYKIIEGRPSNVKLVTTLEDMPSGAPENVHVGMINMTSAFVRWSPPPKNTQNGQLIGYKIQIKSNSSNKILGQMSLNASTTSVIINSLTTGGLYTARVAGQTRVGLGPFSSPTLLNMDPGQL-TQLPPRTDPSHGGASVVRETWFLVLIITMIFTVIAVLLAALYVRRRQAMSKQLGHLN 884 HSP 2 Score: 309.301 bits (791), Expect = 2.062e-84 Identity = 189/486 (38.89%), Postives = 261/486 (53.70%), Query Frame = 0
Query: 21 PVITEHPQNSSVTRNEPVTLRCEAQGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELELDPSICWVQ----KIQGNSPSEEQKEK---EKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHT----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQT 487
P ITEHP + V +NEPVTL C+A+G PEP I+W+KDGE V T+ D KSHRVLLP+GSLFFLR + KKE DGG+YWC A N G V SRNA+L VAVL+D+FR EPQ+ G TA+ ECGPP+G P PT WK++G I++LE I V I P+++ K + E + ++ + LT+ P P + ++A +F C G + R+ GK P R I + +L I +V P+D+G Y C A+N G +SA A L++ P P++ + AG V F CA +G P P I W+R M P + T+ L++ V +DE Y C A ++AG A+ LQV S PP+ + P +Q S +PC A G P P + W KEG ++ PG G + ++ G L I + DSG+YTC + SESG S+ + I T
Sbjct: 28 PRITEHPSDIIVAKNEPVTLNCKAEGKPEPVIEWYKDGELVQTSPGDTKSHRVLLPTGSLFFLRVMHGKKEQDGGVYWCVARNKAGSVPSRNATLTVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDLEATKRITLVDGGNLMISDVRPADQGKYQCIAENMVGTKESAVAV--LTVHVKPYFLAMPSNQTILADQTAEFACRVGGDPEPEILWRR--NDGKMPIGR--AHILNDKSLHIERVNPQDQGTYICDAENGVGA-ISASATLTVHSRPVFTNFPKDETVNAGTDVTFSCAARGAPKPSIFWTREGSQELMFPGHTYQSHYTVTEDGSLSIKGAVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEG--VLVRPG---GRITMAINGTLFIDDLHANDSGLYTCIASSESGNTSWSAYLTIST 501
BLAST of roundabout 1 vs. nr
Match: gi|307209011|gb|EFN86211.1| (Roundabout-like protein 2 [Harpegnathos saltator]) HSP 1 Score: 447.588 bits (1150), Expect = 1.869e-134 Identity = 266/754 (35.28%), Postives = 391/754 (51.86%), Query Frame = 0
Query: 255 EARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGMLPAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLLPGSN-QGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESGAILASSEVKIQTSQDRPPPIIQIGPVNQTQRIGSSTNFPCEA--TPQTRVEWLKDGVPISTMNDPRIKVDHDNTLHFEELEISDSALYTCQAKSTSGQAMLSAFLRVAPAEENVEFDSVPFLSEFPSSPSEPYLINATSNQLVIGWDKPIRIGGSPIQSYQVREGHCWKSFPLSITVQIWDPIVFHLSSFQIEYYTSRTPTHWVAIPEVQSSEGFTLYDFEPATTVHVLVRARNLHGLSPPSPISKPFQTPPKAGEDISDPRYLRSKLS-DKRVMELKEVKILGSRKVKLSWELLIPQKFVTGYHIQLRDISDLDSSDSYDVITISGGGTRSHTLTDLRPNTEYSVFMLPYNGRIKGRPTKLVMVTTKEDVPTAAPENVKIQMLNLTAAVIQWHEPPRRDLHGDLKGFKVLIDINGTEAL--NFTLEPSSNSLILYNLTEGLTYAVQVAAYNRQGVGPLCDPVELEVETDLFHPQLVPEKAP--GMDLVVQEVWF--VMVVSFFSVILLVGFLAMVCFRRIRQDGKHMGHYN 998
EA +++ G L+IS V+ D+G+YQC+A+N+ G + SA A L++ V P+ L P N + + T F C V G P PEI W R +G +P R H D L + + P+D Y C AEN G + ASA L V S PVF P D+ G+ V I C A G P P +FW +EG ++ PG+ Q H V+ +G L I T KD G Y C+++S++GA A+ +++ + ++ PPPII++GP NQT + S + PC A TP RV W K+GV + RI + + TL ++L +DS LYTC A S SG SA+L V+ F P +S P +PS+P ++NATSN + + W P G S I Y V EYY+S T WV + +T+ D P T LVRA N HGLS P P+S QT + R++ + ++ LKEV+ L S VK+ W++L V G +I+ R++S+ Y ++T+ G S+ L LR + Y F++P+ I+GRP+ + + TT EDVP+ APENV + M+N+T+A ++W PP+ +G L G+K+ I N + + +L S+ S+I+ +L G Y +VA R G+GP P L ++ QL P P G VV+E WF +M+ F+VI + LA + RR + K +GH N
Sbjct: 78 EATKRITLVDGGNLMISDVRQTDQGKYQCIAENMVGAKESAIATLTVHVKPFFLTTPSNQTILTDQTAEFACRVGGDPPPEILWRRSDGKMPIGRAHILDDKSLRIERVNPQDHGTYICQAENGVGTISASATLTVHSKPVFTNFPKDETVSAGTDVTISCAARGAPKPSIFWTREGSQELMFPGNTYQSHYTVTEDGSLSIKATVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGG--RITMAINGTLFIDDLRSNDSGLYTCIASSESGNTSWSAYLTVSAG---ASFHRTPDISALPQNPSKPRIVNATSNSVTLTW-SPGHEGQSKILGYNV------------------------------EYYSSNLNTGWVVAATGVLDDIYTVTDLRPETNYVFLVRAENSHGLSLPGPLSDVVQTTNVNQHTVPQVELTRARDRLNSEILHLKEVQALSSTSVKIMWDILGAADLVEGLYIRYREVSE---KPEYQMVTVLNAGATSYVLASLRKYSRYEFFLVPFYKIIEGRPSNVKLATTMEDVPSGAPENVHVGMINMTSAYVRWSPPPKNTQNGQLIGYKIQIKSNSSNKILGQMSLNASTTSVIINSLNTGGLYTARVAGLTRIGLGPFSSPTLLNMDPGQL-TQLPPRTDPSHGDASVVRETWFLVLMITMVFTVIAV--LLAALYVRRRQAMSKQLGHLN 789 HSP 2 Score: 182.185 bits (461), Expect = 6.287e-43 Identity = 129/391 (32.99%), Postives = 189/391 (48.34%), Query Frame = 0
Query: 105 FGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKKDGRILELE-------LDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGTESLTLSYCPKLDTRPQIELVVACNREDFDCPKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVLPYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNG---MLPAQRVHT----TDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFS-----PPVFLVKPTDQKGVQGSTVQIPCVAAGNPVPILFWMKEGGSGVLL-PGSNQGHVHVSPEGMLRIGNTSEKDSGVYTCASVSESG 475
+++ R A L VAVL+D+FR EPQ+ G TA+ ECGPP+G P PT WK++G I++LE +D + ++ + Q E + A++ I T LT+ P T P + ++ +F C G + R+ GK P R I + +L I +V P+D G Y C A+N G +SA A L++ P P++ + AG V CA +G P P I W+R M P + T+ L++ V +DE Y C A ++AG A+ LQV S PP+ + P +Q S +PC A G P P + W KE GVL+ PG G + ++ G L I + DSG+YTC + SESG
Sbjct: 17 LSQIQWRIAGLSVAVLRDEFRAEPQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDLEATKRITLVDGGNLMISDVRQTDQGKYQCIAENMVGAKESAIAT--LTVHVKPFFLTTPSNQTILTDQTAEFACRVGGDPPPEILWRR--SDGKMPIGR--AHILDDKSLRIERVNPQDHGTYICQAENGVGT-ISASATLTVHSKPVFTNFPKDETVSAGTDVTISCAARGAPKPSIFWTREGSQELMFPGNTYQSHYTVTEDGSLSIKATVRKDEGHYVCSAISQAGASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKE---GVLVRPG---GRITMAINGTLFIDDLRSNDSGLYTCIASSESG 394 HSP 3 Score: 86.6557 bits (213), Expect = 1.890e-13 Identity = 100/396 (25.25%), Postives = 160/396 (40.40%), Query Frame = 0
Query: 27 PQNSSVTRNEPVTLRCEA-QGDPEPQIKWFKDGEPVVTAAADHKSHRVLLPSGSLFFLRAVQSKKENDGGIYWCEATNTFGRVRSRNASLVVAVLKDKFRIEPQDVGAIQGGTAIFECGPPKGSPTPTSFWKK-DGRI---LELELDPSICWVQKIQGNSPSEEQKEKEKLFEARKDEIGT----ESLTLSYCPKLDTRPQIELVVACNREDFDC-----PKGLEAIMGNFGRQLIRLGKRPEARFLLSIEGEGTLIISQVQPKDEGQYQCVAKNVAGIRLSAKARLSLQVL-------PYVLKHPENVSAVAGDTVRFGCAVKGRPHPEIRWSRRNGML-PAQRVHTTDGTQLTLMNIVPEDEDVYTCLAENRAGFVEASAHLQVFSPPVFLVKP 400
PQN+ V E L C +G PEP + W ++G + A + R+ L G + V ++ D G Y C A N G S A+L V V K F P + + TA F C G P P W++ DG++ LD ++++ Q + +A ++ +GT +LT+ P P+ E V A C PK ++L+ G ++ + ++ +G+L I KDEG Y C A + AG ++ A + LQV P + P N + C G P P + W + ++ P R+ L + ++ D +YTC+A + +G SA+L V + F P
Sbjct: 40 PQNTRVAAGETALLECGPPRGHPEPTLHWKRNGHIIDLEA----TKRITLVDGGNLMISDV---RQTDQGKYQCIAENMVGAKESAIATLTVHV-KPFFLTTPSNQTILTDQTAEFAC-RVGGDPPPEILWRRSDGKMPIGRAHILDDKSLRIERVN------PQDHGTYICQA-ENGVGTISASATLTVHSKPVFTNFPKDETVSAGTDVTISCAARGAPKPSIFWTREGSQELMFPGNTYQSHY--TVTEDGSLSIKATVRKDEGHYVCSAISQAG---ASTATVFLQVTSVEETPPPIIELGPANQTLPLKSVASLPCRAVGTPTPRVHWHKEGVLVRPGGRITMAINGTLFIDDLRSNDSGLYTCIASSESGNTSWSAYLTVSAGASFHRTP 414 The following BLAST results are available for this feature:
BLAST of roundabout 1 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 25
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BLAST of roundabout 1 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of roundabout 1 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold49_size462716:377790..401551+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold49_size462716-snap-gene-3.30 ID=maker-scaffold49_size462716-snap-gene-3.30|Name=roundabout 1|organism=Tigriopus kingsejongensis|type=gene|length=23762bp|location=Sequence derived from alignment at scaffold49_size462716:377790..401551+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'roundabout 1' has the following synonyms
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