BLAST of Vinculin vs. L. salmonis genes
Match: EMLSAG00000003628 (supercontig:LSalAtl2s:LSalAtl2s198:187462:224170:-1 gene:EMLSAG00000003628 transcript:EMLSAT00000003628 description:"maker-LSalAtl2s198-augustus-gene-2.7")

HSP 1 Score: 825.469 bits (2131), Expect = 0.000e+0
Identity = 438/795 (55.09%), Postives = 579/795 (72.83%), Query Frame = 0

Query:    2 PMYQTKTIAGILEPVAQQVSKLIILHEEGEDGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQV----KKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGR-GTEEAVENRNYLAKRMTDEMGEIVKILEDS-TRSETAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQE-KRKLLQICNDLDQANRQRNIHDPRS-RQNVADLIRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSN--ETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFAN--ARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSR---SVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVS--DGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLP---TDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQV 776
            PMYQT+TIA ILEPVAQQVSKLIILHEEGEDGNAMPDLE+PV+AVS AV NL +VGKDT+NSSQD KLK+EMP +L +IE AA+ LE AC  +K DP+SQ GRDQLI GSRGILQGTT+ML+ FD+SQV    ++ICRD K++LDYLA++EVIETMEDLVQ+VKDLSPCL+K+C+++D RR DLLNPYHR+SL  +L+Q+KTLAPILICSMKIFIQI G + + G EEA+ENRNYLA+RMT E+ EIV++LEDS  R   A             +++F E++KK+   L  +S+    ++ +L++  V+LG KI +GFD+   +  +L +CN+L  +  +    DP + R+ ++DL    E  + EAVI +II DMA I TP+K + D+V+    +++  KR   D + Q+LK+F+NRL+KTAN+V++ +   +DKRR+  L+    Q+Q LTPQ+INAGTI++NYP+N+AAEENFENL NQ+ EGI  IRD+CDE+V+  IFL+ T+  I++AIQ CE  +  R   S  ++I+N+  AARL+NRLL+ L +EA+NSEDP L  +V  +GD LK ++ PF+ NS+ +ATN+S  D   V  WR    + ++ +  V+R+FD+     F+   D+  Q          P     +NM        PPRPP P   T +R P R P P GCDTDDE+GLF + PAGN+PIH AAHGLYQE++QWDH+DNEIIAAAKKIA+LMAHLSEL++ G+GTK+EL+ACA+GLA+ SE+ITELAKE  KHCTDKKIR NLLQV
Sbjct:    3 PMYQTQTIASILEPVAQQVSKLIILHEEGEDGNAMPDLEKPVQAVSDAVANLTRVGKDTVNSSQDAKLKTEMPNALNSIECAANYLEKACRIMKHDPYSQEGRDQLITGSRGILQGTTNMLLAFDQSQVIFWSERICRDSKRILDYLAISEVIETMEDLVQYVKDLSPCLAKICKDIDGRRNDLLNPYHRDSLAQNLEQIKTLAPILICSMKIFIQINGSDNKKGAEEAIENRNYLARRMTGEIAEIVRVLEDSAVRKSVA-------------DLSFQELVKKI---LNDRSTP---IDLKLLRRAVELGEKIGEGFDAGHVQADVLSLCNELKSSFGK----DPMTIRKYLSDL----ENKIKEAVIDQIIQDMAXIVTPIKLYCDSVNETNYQSLHRKREYADIRGQSLKIFANRLSKTANIVSYFDNETQDKRRAEHLRYLGHQIQNLTPQIINAGTIKINYPDNRAAEENFENLTNQFAEGIQNIRDICDETVNLNIFLRRTEEFIRRAIQNCESIIIHRDKGSSKVIIDNAAAAARLSNRLLLTLSREAENSEDPKLHSRVQSSGDRLKESLGPFLENSRVIATNLSALDRGFVAAWRSATAKFLEAIAGVSRIFDE-----FNDNIDHSSQI---------PNNHFSSNMVPEEEDVPPPRPPPPDPRTFSRAPPRLPIPHGCDTDDEEGLFSNEPAGNQPIHLAAHGLYQEIKQWDHSDNEIIAAAKKIAYLMAHLSELIKGGKGTKKELIACAKGLADQSEKITELAKELAKHCTDKKIRVNLLQV 756          

BLAST of Vinculin vs. L. salmonis genes
Match: EMLSAG00000007901 (supercontig:LSalAtl2s:LSalAtl2s467:121584:148044:-1 gene:EMLSAG00000007901 transcript:EMLSAT00000007901 description:"maker-LSalAtl2s467-snap-gene-1.52")

HSP 1 Score: 110.923 bits (276), Expect = 4.851e-25
Identity = 70/186 (37.63%), Postives = 93/186 (50.00%), Query Frame = 0

Query:  673 TDDE-DGLFLSAPAGNR-PIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            TDDE +  FLSAP  ++ PI          VRQW   DN+IIAAAKK+A LMA LS+LV    G+KREL+ CA+ +AE S+ +T +AKE  K CTDK++RT                                                    +V+ TV  AE+ S ++R+DAG +LKW+RK  PW
Sbjct:  940 TDDESEDFFLSAPTPSQGPIM---------VRQWSSKDNDIIAAAKKMAHLMAKLSQLVGEDSGSKRELITCAKSIAEASDDVTRIAKELAKECTDKRMRT----------------------------------------------------SVKHTVTVAEAXSXKIRTDAGIRLKWVRKQ-PW 1063          

HSP 2 Score: 97.4413 bits (241), Expect = 6.845e-21
Identity = 83/315 (26.35%), Postives = 148/315 (46.98%), Query Frame = 0

Query:  291 DGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQRNIHD-------PRSR-QNVADLIRQLEVSVNEAVIG----RIIHDMADITTPLKHFTDAVHSNETISMKRMSVD-EKSQTLKVFSNRLAKTANLVAFANARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQ 592
            D  L Q  I  I+D   KI++     ++  +  +C  ++  +  R++ D         SR QN+A  I     ++ +A++     R++ D  D+  PLK  TD V +    S+ R  +  EKS  L + S+ + +TA +VA  N                         ++G        NK   E      +QY E     R LCD++VD++ F+  + + ++   + CE  +R  +   L+E +   ARLANR+  V H+EA+NSE+P     +  + + L+  +   + ++KALA N+++ S +K+WR+ +Q
Sbjct:  173 DRGLGQHAINLILDEARKIAELLPPNQRDSVRSLC--IETEDDIRDLKDLCTSGYAGSSRAQNLARQIATRLDAIKDAILSVLVERVVKDFMDVGYPLKKLTDCVQNPVGASLDRDRIFIEKSDKLSLSSDNIGRTAKMVALGN-------------------------DSG--------NKKLAEGLLAAASQYIETNDRARALCDDAVDSRDFVAKSLSAMEADARRCETCVRDSNSLGLVEEAMNIARLANRVFQVAHQEAENSEEPAFISTLNISAEKLQSRLQIMLQSAKALALNLTNESAIKDWRKQSQ 452          

BLAST of Vinculin vs. L. salmonis genes
Match: EMLSAG00000008522 (supercontig:LSalAtl2s:LSalAtl2s525:1238:6310:1 gene:EMLSAG00000008522 transcript:EMLSAT00000008522 description:"maker-LSalAtl2s525-augustus-gene-0.13")

HSP 1 Score: 87.0409 bits (214), Expect = 9.851e-18
Identity = 155/742 (20.89%), Postives = 316/742 (42.59%), Query Frame = 0

Query:  145 AVAEVIETMEDLVQFVKDLS--PC-------------LSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVE--NRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRK--LLQICNDLDQA------------NRQRNIHDPRSRQNVADLIRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAV-HSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDH-LKGAITP-FVTNSKALATNVSDGSLV-----KNWRQGAQRLV-DMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDAR--LPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRG---TKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETPEDQEAMD---MLVFNAEKLNQAVRETVGAAESASIR 838
            AV +V++T E +    ++ +  PC              S++  +   R+ +L +P  R+ L      +K  + +L  + K ++       R  E A    NR+Y+ +++ + +  I  +    + SE  + + +G   +      F +++  ++  L    S+     ++ ++ I+     I+D   ++++RK  ++  CN + QA             +Q + +  R+ +++    R L   + +AV+  +     D   PL     A    NE        V+E ++     +++L + + L    +A ++     ++  ++Q++ L PQ++NA  +    P++K A+EN +  +  + E + ++ D  D+      FL  ++  I + +  C   L+S     L   +T     ++R+  V+  E DN E PG+  + +      L+G + P F    ++    +S  S V     +N    A RLV D V E+ R      +       D    T+ EI ++     +P  + T + +   P    + T+AR  + K P         +ED   ++       +  +     QEV +WD   N+II  AK +  +M  +++  + GRG   T   ++  A+ ++E   ++ +LA++    C +   + +L    ++I     QL + + VKA +  +NV        +D    L+  A+ L  AV  TV A+  AS +
Sbjct:   97 AVEQVLQTGETMSSAAREFATDPCSSIKRGNMRFGESASELMNQAAKRQYELKDPALRDDLAAARAVLKKHSMMLXTASKAYV-------RHPELAAAKANRDYVLRQVCEAVNTISDVARGKSPSEW-NGLGDGPGELAAALDDFDDLI--ITNPLIYNESRTRPCFEERLESIISGAALIADASCTRDERKDRIVAECNSVRQALQDLLSEYMSNAGKQPSANLDRAIESMQKKTRDLRRQLRKAVVDHVSDSFLDCNVPLHVMIAAAKEGNEK------DVEEYARIFVDHADKLVEVSKLACSMSA-NEDGVKMVKYAASQIEKLCPQVVNAAKVLSARPKSKVAQENIQVFKEAWEEHVRILTDAVDDITTVDDFLAVSENHILEDVNNCVAALQSGDAGGLDRMATSIQGRSSRICNVVSAEMDNYE-PGVYTERVNEAIRILRGEVMPNFAHKVESAVDMLSSPSPVTDKVDENEFIDASRLVYDGVREIRRAV----LLNRSTEEDVDSDTEWEI-MDERASNQPRPDSTTTEIDEYPEISGI-TNAREAMKKLP---------EEDRAKIAQQVETFKLEKSKFD--QEVSKWDDKGNDIIVLAKHMCMIMMEMTDFTR-GRGPLQTXMAVINAAKKISEAGTKLDKLARQIADQCPESSTKKDLTAYLQRIALHCHQLNITSKVKADV--QNVSGELIVSGLDSATSLIQAAKNLMNAVVLTVKASYVASTK 800          

BLAST of Vinculin vs. SwissProt
Match: gi|50401320|sp|O46037.1|VINC_DROME (RecName: Full=Vinculin)

HSP 1 Score: 416.001 bits (1068), Expect = 6.504e-130
Identity = 263/672 (39.14%), Postives = 401/672 (59.67%), Query Frame = 0

Query:  195 DQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGVSSINTNEMTFHEVMKKLSAS---LASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQRNIHDPRSRQNVADL---IRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANA-RDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDED-GLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELV-QPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSD-AGFKLKWIRKPVPW 856
            D+V  +A  L C ++       QEG+G     E+   L + + D MGE+  ++         H+ + GV      + T H +  +L  +   L      DG L ++ I  IV+ G K+++G    +K ++ Q+C+++++  RQ     P ++Q    L   + +L+ ++  A++ RI+ D  D++TPLK FT+AV   E    +  + ++KS  L+ FS+R +KT+ +VA   A  +K+ +  L   + QV +LTPQLI+AG IRMNYP +KAA+E+ +NL+ QY + +  +R LCD++ D   F++ ++  +Q   + CE+ + +R    +++N++  ARL NR+L+V  +EADNSEDP    ++  A + L+ ++   V ++K +ATN++D +    W+   QRL+  V EV    D +                 E+       A  L+N    N   APPRPPLP +   P RPP P   +TDDED G+F + P  N+PI  AA GL+QEVRQW   DNEIIAAAK++A LMA LSELV    RG+KREL+A A+ +AE SE +T LAKE  + CTD++IRTNLLQVCE+IPT+GTQL++L+TVKATM+     + ED+EA +MLV NA+ L Q+V+ETV AAE ASI++RSD    +L+W+R+  PW
Sbjct:  325 DEVTAMANTL-CELR-------QEGKGQSPQAES---LVRGIRDRMGELKSLV---------HQAVLGVDKAGVQQ-TAHTIQGRLEQAVKWLQHPEINDGGLGERAINLIVEEGRKVAEGCPGHQKAEIQQLCDEVERLKRQAAGSGPAAKQAAKQLTQKLYELKAAIQNALVNRIVQDFMDVSTPLKQFTEAVLQPEGTPGREQNFNQKSNNLQAFSDRASKTSRMVAAGGACGNKKIAEILLSSAAQVDSLTPQLISAGRIRMNYPGSKAADEHLQNLKQQYADTVLRMRTLCDQATDPADFIKTSEEHMQVYAKLCEDAIHARQPQKMVDNTSNIARLINRVLLVAKQEADNSEDPVFTERLNAAANRLERSLPAMVGDAKLVATNIADPAAAAAWKNSFQRLLGDVREVR---DAIAPPQPPPLPTSLPPPIPEL------SALHLSN---QNAERAPPRPPLPREGLAPVRPPPP---ETDDEDEGVFRTMPHANQPILIAARGLHQEVRQWSSKDNEIIAAAKRMAILMARLSELVLSDSRGSKRELIATAKKIAEASEDVTRLAKELARQCTDRRIRTNLLQVCERIPTIGTQLKILSTVKATMLGAQ-GSDEDREATEMLVGNAQNLMQSVKETVRAAEGASIKIRSDQTSNRLQWVRRQ-PW 958          

HSP 2 Score: 339.732 bits (870), Expect = 2.597e-101
Identity = 189/384 (49.22%), Postives = 259/384 (67.45%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGEDGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQD-----------GQLNQQLIQHIVDLGFKISDGF---DSQEKRKL----LQICNDLDQANRQRNIHDPRSR---QNVADLIRQLEVSVNEAVIG 363
            MP++ TKTI  IL+PVAQQVS+L+ILHEE EDGNAMPDL RPV+ VS AV NL KVG+DTINSS D  L+ +MP +L  +E A+ LLE A   L+ DP+S   R +LI GSRGILQGT+S+L+ FDES+V+KI ++CK+VLDYLAVAEVI TME LVQF+KDLSPCLSKV +EV  R K+L +  H E L   L+QVKTLAPILICSMK++I I+ Q+GRG EEA ENRNYLA RM+DE+ EI+++L+ +T  E            +T+E+    V+KKLS ++++K  Q            G + ++ ++ ++D   +IS+     DS   RKL      + N L +  ++     P++    + + D + +L+  V++AV+G
Sbjct:    1 MPVFHTKTIESILDPVAQQVSRLVILHEEAEDGNAMPDLSRPVQVVSAAVANLVKVGRDTINSSDDKILRQDMPSALHRVEGASQLLEEASDMLRSDPYSGPARKKLIEGSRGILQGTSSLLLCFDESEVRKIIQECKRVLDYLAVAEVINTMEQLVQFLKDLSPCLSKVHREVGAREKELTHQVHSEILVRCLEQVKTLAPILICSMKVYIHIVEQQGRGAEEAAENRNYLAARMSDELQEIIRVLQLTTYDE------------DTSELDNLTVLKKLSNAISNKMEQANEWLSNPYALRGGVGEKALRQVIDNATEISERCLPQDSYPIRKLADEVTAMANTLCELRQEGKGQSPQAESLVRGIRDRMGELKSLVHQAVLG 372          

BLAST of Vinculin vs. SwissProt
Match: gi|50401417|sp|Q17162.1|VINC_BRUMA (RecName: Full=Vinculin)

HSP 1 Score: 321.242 bits (822), Expect = 3.146e-94
Identity = 200/588 (34.01%), Postives = 326/588 (55.44%), Query Frame = 0

Query:  316 QEKRKLLQICNDLDQANRQ------RNIHDPRS----RQNVADLIRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFAN-ARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRP---------------------PGPRGCDTDDEDGLFLSAP---AGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVE----------TPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPWIN 858
            Q  RK + +C ++D+   Q      R + +P +    R  + + +R+L   + + +  R++ D ADI+TPLK F DAV+++ TI  + +S +EK+  L   S+R A TA LVA     ++K+   ++ + + QV  +TPQ+I AG IR+ + ++ +A  +F+NLR +Y++ ++ +R   D+++DT  F++ ++  +++    CE  +R+     +++N++  AR  NR+LM    EADNSE+P    +V  A   L  AI P V  +K +A N   G   ++WR   + L+  V +V    D +   G   P                    P  N+ + ++ P  P  P+  D R+  R                      P P   D ++    +   P   A ++PI  AAH L+QE+RQW   +NEI+AAAK++A LMA LS+LV+   GTK++L+ CA+ +A+ SE +T LA +  + CT  K+R  LLQVCE+IPT+ TQL++L+TVKATM+               + ED+EAM  LV NA+ L Q+V++TV AAE+ASI++R+++G +L+WIRKP+ W N
Sbjct:  431 QNVRKFVDLCVEIDRQADQLADLEHRGLGNPPAAHAIRNQLRNKLRELVDIMKKVITDRVVEDFADISTPLKQFVDAVYASPTIVNRELSFEEKAHNLDDHSSRCANTALLVAKCGPCKNKKTVEAIIEAANQVNAMTPQVIKAGKIRL-HNDSDSANLHFDNLRREYSDVLNRLRSHVDDAIDTSDFIRASEQAMRQYTVYCENAIRNNEPQQMVDNTSQIARFGNRVLMTAKNEADNSEEPSFVHRVNNAARRLHSAIPPMVNQAKQVALNPRHGGNAQSWRDANEHLLSAVRQVG---DAITGAGGSRP--------------------PSQNLLVESVPPKAPTSPIVHD-RIYIREDIPTPPRPPPPVEISPPPRPPPPPETDDEEETRAFWERYPLLGASSQPILSAAHNLHQELRQWSSHENEIVAAAKRMAILMARLSQLVRGEGGTKKDLVDCAKAIADSSEEVTRLAVQLARQCTVIKMRMTLLQVCERIPTIATQLKILSTVKATMLGSQATIGPYGQPIDGSEEDEEAMQQLVLNAQNLMQSVKDTVRAAEAASIKIRTNSGLRLRWIRKPM-WSN 992          

HSP 2 Score: 318.931 bits (816), Expect = 1.989e-93
Identity = 165/319 (51.72%), Postives = 222/319 (69.59%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGEDGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSE---------TAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKIS 310
            MP++ TKTI GILEPVAQQVS+L+ILHEE EDGNA+ DL  PV AVS AVDNL KVG DT +SS D  L+++MP +L  +E ++ LLE AC  L+ DP+S   R +LI G+RGILQGT+++L+ FDES+V+KI R C+KVLDYLAVAEVIE+M+DL QFVKD+SP L++V + +D R K+L +  HRE L   +D VKTL+PI+IC+MKIFIQI  +  RG +EA ENRNYLA+RMTDEM EI+++L+ +T  E            K ++   S+ T+ + +          LA    + G   ++ I+ IVD   +I+
Sbjct:    1 MPVFHTKTIEGILEPVAQQVSRLVILHEEAEDGNAVLDLTLPVGAVSRAVDNLIKVGYDTCHSSDDRILQADMPPALQRVEASSRLLEDACHMLRADPYSSVARKKLIEGARGILQGTSALLLSFDESEVRKIIRGCRKVLDYLAVAEVIESMDDLAQFVKDISPWLTRVSRNIDAREKELTHQVHREILLRCMDTVKTLSPIMICAMKIFIQITEESQRGQQEAAENRNYLAQRMTDEMNEIIRVLQLTTYDEDEWDSDNVTVMRKALSAAQSLLTSALDW----------LADSRGRAGATGEKAIRRIVDYSERIA 309          

BLAST of Vinculin vs. SwissProt
Match: gi|50403784|sp|Q64727.4|VINC_MOUSE (RecName: Full=Vinculin; AltName: Full=Metavinculin)

HSP 1 Score: 322.013 bits (824), Expect = 4.897e-94
Identity = 204/580 (35.17%), Postives = 315/580 (54.31%), Query Frame = 0

Query:  291 DGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQ-------RNIHDPRSR---QNVADLIRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAG---NRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSE-NVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            D  + Q  I+ +V  G ++++      ++ LL  C+ +DQ   Q            P++R     + D ++ L+  + EA+   +    +D TTP+K    A  +      +    DE++   +  S RL  TA   A     +K     +Q      + LTPQ+I+A  I +  P N+AA E+FE ++NQ+ + +  +  L DE++DTK  L  ++  I+K +  C+  + +    +L+  +T  AR ANR+L+V  +E +NSEDP  R  V  A D L   I+P V ++KA+A N+SD  L K++     R++  V +V   F                                L  + +++ + APP+PPLP     P RP        +++D  F    AG   N+P+  AA  L+ E R+W    N+IIAAAK++A LMA +S LV+ G GTKR L+ CA+ +A+ S+ +T LAKE  K CTDK+IRTNLLQVCE+IPT+ TQL++L+TVKATM+   N+   E ++A +MLV NA+ L Q+V+ETV  AE+ASI++R+DAGF L+W+RK  PW
Sbjct:  511 DRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPP-------------------PPDLEQLRLTDEL-APPKPPLPEGEVPPPRP-----PPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRK-TPW 1064          

HSP 2 Score: 256.914 bits (655), Expect = 7.646e-71
Identity = 143/350 (40.86%), Postives = 220/350 (62.86%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGE-DGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKIL------EDSTRSETAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQRNIHDPRSR 343
            MP++ T+TI  ILEPVAQQ+S L+I+HEEGE DG A+PDL  PV AV  AV NL +VGK+T+ +++D  LK +MP + + +E A   L  A   L+ DP+S   RD LI GSRGIL GT+ +L+ FDE++V+KI R CK +L+YL VAEV+ETMEDLV + K+L P ++K+ + +D R+++L +  HR  L   ++ VK L P+LI +MKIF+     + +G EEA++NRN+  ++M+ E+ EI+++L      ED+  S+    M   ++SI++        + +    L   ++  G   +Q I+ I+D   K+ +    +E+R++L  C  L Q   Q  + D R+R
Sbjct:    1 MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSK-------LNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQ--VADLRAR 341          

BLAST of Vinculin vs. SwissProt
Match: gi|205830826|sp|P85972.1|VINC_RAT (RecName: Full=Vinculin; AltName: Full=Metavinculin)

HSP 1 Score: 311.997 bits (798), Expect = 1.979e-90
Identity = 192/513 (37.43%), Postives = 289/513 (56.34%), Query Frame = 0

Query:  348 DLIRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAG---NRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSE-NVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            D ++ L+  + EA+   +    +D TTP+K    A  +      +    DE++   +  S RL  TA   A   A +K     +Q      + LTPQ+I+A  I +  P N+AA E+FE ++NQ+ + +  +  L DE++DTK  L  ++  I+K +  C+  + +    +L+  +T  AR ANR+L+V  +E +NSEDP  R  V  A D L   I+P V ++KA+A N+SD  L K++     R++  V +V   F                                L  + +++ + APP+PPLP     P RP        +++D  F    AG   N+P+  AA  L+ E R+W    N+IIAAAK++A LMA +S LV+ G GTKR L+ CA+ +A+ S+ +T LAKE  K CTDK+IRTNLLQVCE+IPT+ TQL++L+TVKATM+   N+   E ++A +MLV NA+ L Q+V+ETV  AE+ASI++R+DAGF L+W+RK  PW
Sbjct:  578 DSLKDLKTQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGAANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPP-------------------PPDLEQLRLTDEL-APPKPPLPEGEVPPPRP-----PPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRK-TPW 1064          

HSP 2 Score: 256.144 bits (653), Expect = 1.347e-70
Identity = 143/350 (40.86%), Postives = 219/350 (62.57%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGE-DGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKIL------EDSTRSETAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQRNIHDPRSR 343
            MP++ T+TI  ILEPVAQQ+S L+I+HEEGE DG A+PDL  PV AV  AV NL +VGK+T+ +++D  LK +MP + + +E A   L  A   L+ DP+S   RD LI GSRGIL GT+ +L+ FDE++V+KI R CK +L+YL VAEV+ETMEDLV + K+L P ++K+ + +D R+++L +  HR  L   ++ VK L P+LI +MKIF+     + +G EEA++NRN+   +M+ E+ EI+++L      ED+  S+    M   ++SI++        + +    L   ++  G   +Q I+ I+D   K+ +    +E+R++L  C  L Q   Q  + D R+R
Sbjct:    1 MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVGKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSK-------LNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQ--VADLRAR 341          

BLAST of Vinculin vs. SwissProt
Match: gi|138545|sp|P19826.1|VINC_CAEEL (RecName: Full=Vinculin; AltName: Full=P107B)

HSP 1 Score: 273.863 bits (699), Expect = 4.258e-77
Identity = 157/361 (43.49%), Postives = 226/361 (62.60%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGEDGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGR-GTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSE---------TAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGF---DSQE-KRKLLQICNDLDQANRQRNIHDPRSRQNVA 347
            MP++ TKTI  ILEPVAQQVS+L+ILHEE  DGNAMPDL  PV  VS AV NL +VG DT + S D  L+ +MP +L  +E ++ LLE +   LK DP+S   R +LI G+RGILQGT+++L+ FDES+V+KI R C+K  DY+AV+EVIE+M DL QFVKD+SP L  V  +V+ R+++L +  HRE L   +D +K +APILICSMK  I++     R G  EA+ NRN++++RMT+EM EI+++L+ +T  E            K ++   S+ T  + +          LA   ++ G + ++ I+ I +   +IS      D+Q  KR + +I +  D+    RN   P  R+N+A
Sbjct:    1 MPVFHTKTIENILEPVAQQVSRLVILHEEANDGNAMPDLTGPVGMVSRAVGNLIQVGYDTCDHSDDRILQQDMPPALQRVEGSSKLLEESSYSLKHDPYSVPARKKLIDGARGILQGTSALLLCFDESEVRKIIRVCRKANDYVAVSEVIESMADLQQFVKDISPVLHDVTNDVNLRQQELTHQVHREILIRCMDSIKVIAPILICSMKTSIELGTPHPRQGHAEAIANRNFMSQRMTEEMNEIIRVLQLTTYDEDEWDADNVTVMRKALSAAKSLLTAALDW----------LADPHARSGAVGEKAIRRICEYADRISARALPEDAQSIKRSIFEITSFTDELCNLRNNGQP-DRENLA 350          

HSP 2 Score: 180.644 bits (457), Expect = 1.644e-45
Identity = 91/184 (49.46%), Postives = 134/184 (72.83%), Query Frame = 0

Query:  685 AGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMM-SENVETP---------EDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPWIN 858
            A ++P+  AAH L+ E++QW   +N+I+AAAK++A LMA LS+LV+   GTK++L+ C++ +A+ SE +T LA +  + CTD K+RT LLQV E+IPT+ TQL+VL+TVKATM+ S NV  P         ED EAM  LV NA+ L Q+V++ V AAE+ASI++R+++G +L+W+RKP+ W N
Sbjct:  827 ASHQPMLAAAHNLHNELKQWSSQENDIVAAAKRMAILMARLSQLVRGEGGTKKDLINCSKAIADSSEEVTRLAVQLARLCTDIKMRTALLQVSERIPTIATQLKVLSTVKATMLGSANVIGPYGQPVEGSEEDDEAMQQLVHNAQNLMQSVKDVVRAAEAASIKIRTNSGLRLRWLRKPM-WSN 1009          

BLAST of Vinculin vs. SwissProt
Match: gi|50403716|sp|P12003.4|VINC_CHICK (RecName: Full=Vinculin; AltName: Full=Metavinculin)

HSP 1 Score: 266.929 bits (681), Expect = 3.431e-74
Identity = 143/350 (40.86%), Postives = 218/350 (62.29%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGE-DGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKIL------EDSTRSETAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQRNIHDPRSR 343
            MP++ T+TI  ILEPVAQQ+S L+I+HEEGE DG A+PDL  PV AV  AV NL +VGK+T+ +++D  LK +MP + + +E A   L  A   L+ DP+S   RD LI GSRGIL GT+ +L+ FDE++V+KI R CK +L+YL VAEV+ETMEDLV + K+L P ++K+ + +D R+++L +  HR  L   ++ VK L P+LI +MKIF+     + +G EEA++NRN+  ++M+ E+ EI+++L      ED+  S+    M   ++ I++        M +    L   ++  G   +Q I+ I+D   K  +    +E+R++L  C  L Q   Q  + D R+R
Sbjct:    1 MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVSAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVRAAQMLQADPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNTKSQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSK-------MNQAKGWLRDPNAPPGDAGEQAIRQILDEAGKAGELCAGKERREILGTCKTLGQMTDQ--LADLRAR 341          

HSP 2 Score: 199.904 bits (507), Expect = 1.097e-51
Identity = 94/171 (54.97%), Postives = 129/171 (75.44%), Query Frame = 0

Query:  687 NRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSE-NVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            N+PI  AA  L++E  +W    N+IIAAAK++A LMA +S LV+ G G KR L+ CA+ +A+ S+ +T LAKE  K CTDK+IRTNLLQVCE+IPT+ TQL++L+TVKATM+   N+   E ++A +MLV NA+ L Q+V+ETV  AE+ASI++R+DAGF L+W+RK  PW
Sbjct:  964 NQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRK-TPW 1133          

HSP 3 Score: 137.887 bits (346), Expect = 7.245e-32
Identity = 81/269 (30.11%), Postives = 139/269 (51.67%), Query Frame = 0

Query:  340 PRSRQNVADL---IRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLF 605
            P++R   A L   ++ L+  + EA+   +    +D TTP+K    A  +      +    +E++   +  + RL  TA   A     +K     +Q      + LTPQ+++A  I +  P N+AA E+FE ++NQ+ + +  +  L DE++DTK  L  ++  I+K +  C+  + +    +L+  +T  AR ANR+L+V  +E +NSEDP  R  V  A D L   I+P V ++KA+A N+SD  L K++     R++  V +V   F
Sbjct:  567 PQARAIAAQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPSDTPNREEVFEERAANFENHAARLGATAEKAAAVGTANKTTVEGIQATVKSARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANMQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAF 835          

BLAST of Vinculin vs. SwissProt
Match: gi|21903479|sp|P18206.4|VINC_HUMAN (RecName: Full=Vinculin; AltName: Full=Metavinculin; Short=MV)

HSP 1 Score: 258.455 bits (659), Expect = 3.277e-71
Identity = 144/350 (41.14%), Postives = 220/350 (62.86%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGE-DGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKIL------EDSTRSETAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQRNIHDPRSR 343
            MP++ T+TI  ILEPVAQQ+S L+I+HEEGE DG A+PDL  PV AV  AV NL +VGK+T+ +++D  LK +MP + + +E A   L  A   L+ DP+S   RD LI GSRGIL GT+ +L+ FDE++V+KI R CK +L+YL VAEV+ETMEDLV + K+L P ++K+ + +D R+++L +  HR  L   ++ VK L P+LI +MKIF+     + +G EEA++NRN+  ++M+ E+ EI+++L      ED+  S+    M   ++SI++        + +    L   S+  G   +Q I+ I+D   K+ +    +E+R++L  C  L Q   Q  + D R+R
Sbjct:    1 MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSK-------LNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQ--VADLRAR 341          

HSP 2 Score: 202.601 bits (514), Expect = 1.440e-52
Identity = 102/194 (52.58%), Postives = 138/194 (71.13%), Query Frame = 0

Query:  666 PGPRGCDTDDEDGLFL--SAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSE-NVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            P P   + D E  L L  S    N+PI  AA  L++E  +W    N+IIAAAK++A LMA +S LV+ G GTKR L+ CA+ +A+ S+ +T LAKE  K CTDK+IRTNLLQVCE+IPT+ TQL++L+TVKATM+   N+   E ++A +MLV NA+ L Q+V+ETV  AE+ASI++R+DAGF L+W+RK  PW
Sbjct:  940 PVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRK-TPW 1132          

HSP 3 Score: 145.206 bits (365), Expect = 3.450e-34
Identity = 100/358 (27.93%), Postives = 172/358 (48.04%), Query Frame = 0

Query:  291 DGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQ-------RNIHDPRSR---QNVADLIRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPL 638
            D  + Q  I+ +V  G ++++      ++ LL  C+ +DQ   Q            P++R     + D ++ L+  + EA+   +    +D TTP+K    A  +      +    DE++   +  S +L  TA   A     +K     +Q      + LTPQ+++A  I +  P N+AA E+FE ++NQ+ + +  +  L DE++DTK  L  ++  I+K +  C+  + +    +L+  +T  AR ANR+L+V  +E +NSEDP  R  V  A D L   I+P V ++KA+A N+SD  L K++     R++  V +V   F          P D +   Q  +  E+ PP  PL
Sbjct:  511 DRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLE---QLRLTDELAPPKPPL 865          

BLAST of Vinculin vs. SwissProt
Match: gi|50403675|sp|P26234.4|VINC_PIG (RecName: Full=Vinculin; AltName: Full=Metavinculin)

HSP 1 Score: 256.529 bits (654), Expect = 1.416e-70
Identity = 139/344 (40.41%), Postives = 216/344 (62.79%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGE-DGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQRNIHDPRSR 343
            MP++ T+TI  ILEPVAQQ+S L+I+HEEGE DG A+PDL  PV AV  AV NL +VGK+T+ +++D  LK +MP + + +E A   L  A   L+ DP+S   RD LI GSRGIL GT+ +L+ FDE++V+KI R CK +L+YL VAEV+ETMEDLV + K+L P ++K+ + +D R+++L +  HR  L   ++ VK L P+LI +MKIF+     + +G EEA++NRN+  ++M+ E+ EI+++L+ ++  E A        ++     +    + +    L   ++  G   +Q I+ I+D   K+ +    +E+R++L  C  L Q   Q  + D R+R
Sbjct:    1 MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPTASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQ--VADLRAR 342          

HSP 2 Score: 204.912 bits (520), Expect = 2.764e-53
Identity = 102/194 (52.58%), Postives = 139/194 (71.65%), Query Frame = 0

Query:  666 PGPRGCDTDDEDGLFL--SAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSE-NVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            P P   + D E  L L  S+   N+PI  AA  L++E  +W    N+IIAAAK++A LMA +S LV+ G GTKR L+ CA+ +A+ S+ +T LAKE  K CTDK+IRTNLLQVCE+IPT+ TQL++L+TVKATM+   N+   E ++A +MLV NA+ L Q+V+ETV  AE+ASI++R+DAGF L+W+RK  PW
Sbjct:  941 PVPSDMEDDYEPELLLMPSSQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRK-TPW 1133          

HSP 3 Score: 146.747 bits (369), Expect = 1.216e-34
Identity = 101/358 (28.21%), Postives = 172/358 (48.04%), Query Frame = 0

Query:  291 DGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQ-------RNIHDPRSR---QNVADLIRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPL 638
            D  + Q  I+ +V  G ++++      ++ LL  C+ +DQ   Q            P++R     + D ++ L+  + EA+   +    +D TTP+K    A  +      +    DE++   +  S RL  TA   A     +K     +Q      + LTPQ+++A  I +  P N+AA E+FE ++NQ+ + +  +  L DE++DTK  L  ++  I+K +  C+  + +    +L+  +T  AR ANR+L+V  +E +NSEDP  R  V  A D L   I+P V ++KA+A N+SD  L K++     R++  V +V   F          P D +   Q  +  E+ PP  PL
Sbjct:  512 DRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLE---QLRLTDELAPPKPPL 866          

BLAST of Vinculin vs. SwissProt
Match: gi|418252|sp|Q04615.1|VINC_XENLA (RecName: Full=Vinculin; AltName: Full=Metavinculin)

HSP 1 Score: 199.519 bits (506), Expect = 2.967e-57
Identity = 98/187 (52.41%), Postives = 135/187 (72.19%), Query Frame = 0

Query:  674 DDEDGLFLSAPAG---NRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSE-NVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            DD +   L  P G   N+P+  AA  L++E  +W    N+IIAAAK++A LMA +S LV+ G G KR L+ CA+ +A+ S+ +T+LAKE  K CTDK+IRTNLLQVCE+IPT+ TQL++L+TVKATM+   N+   E ++A +MLV NA+ L Q+V+ETV  AE+ASI++R+DAGF L+W RK  PW
Sbjct:   48 DDYEPELLLVPEGQPVNQPMLAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGNKRALIQCAKDIAKASDEVTKLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWARK-TPW 233          

BLAST of Vinculin vs. SwissProt
Match: gi|75056714|sp|Q5R416.3|CTNA2_PONAB (RecName: Full=Catenin alpha-2; AltName: Full=Alpha N-catenin)

HSP 1 Score: 124.02 bits (310), Expect = 9.547e-28
Identity = 192/895 (21.45%), Postives = 375/895 (41.90%), Query Frame = 0

Query:    5 QTKTIAGILEPVAQQVSKLIILHEEGEDGNAMPDLERP---VKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAV--ENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGVSSINT--NE---------MTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRK--LLQICNDLDQA----------NRQR-------NIHDPRSRQNVADLIRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHS-NETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNS-----KALATNVS---------DGS-LVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRG---TKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVE-TPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAG 844
            +T T+  +LEP+  QV+ L+    +G  G      ++      +V  A  N  + G+     SQD  LK E+  ++  + K  + + +A  +   DP S   R  ++  +R +L   T +L++ D + V ++    K V + L   +     +DL    K+    + K+      R+++L +P+ R+ +      +K  A +L  + + F+       R  + A    NR+Y+ K++ + +  I    + ++ ++ A K   G+  +    NE         MTF E   +   SL           ++ ++ I+     ++D   +++ R+  ++  CN + QA          N  R       NI   +  +   DL RQL     +AV+  I     +   PL    +A  S NE        V E +Q  +  +N+L + ANL A + + ++     ++  +TQ+ +L PQ+INA       P++K A++N +  ++Q+ + + ++ +  D+      FL  ++  I + +  C   L+   V  L   +      A R++ +++ E +N E      +V+ A   L   + P          +AL+ NV          D S LV +  +  ++ V M+     L DD +        DY  +++  +  E     + +A  +   ++           A+LP+           ++  +F          H     L  EV +WD + N+II  AK++  +M  +++  + G+G      +++  A+ +AE   R+ +LA+     C D   + +LL   ++I     QL + + VKA + +   E      ++   L+  A+ L  AV  TV A+  AS + +   G
Sbjct:   20 RTLTVERLLEPLVTQVTTLVNTSNKGPSGKKKGRSKKAHVLAASVEQATQNFLEKGEQIAKESQD--LKEELVAAVEDVRKQGETMRIASSEFADDPCSSVKRGTMVRAARALLSAVTRLLILADMADVMRLLSHLKIVEEALEAVKNATNEQDLANRFKEFGKEMVKLNYVAARRQQELKDPHCRDEMAAARGALKKNATMLYTASQAFL-------RHPDVAATRANRDYVFKQVQEAIAGISNAAQATSPTDEA-KGHTGIGELAAALNEFDNKIILDPMTFSEA--RFRPSL-----------EERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSEYMNNTGRKEKGDPLNIAIDKMTKKTRDLRRQL----RKAVMDHISDSFLETNVPLLVLIEAAKSGNEK------EVKEYAQVFREHANKLVEVANL-ACSISNNEEGVKLVRMAATQIDSLCPQVINAALTLAARPQSKVAQDNMDVFKDQWEKQVRVLTEAVDDITSVDDFLSVSENHILEDVNKCVIALQEGDVDTLDRTAGAIRGRAARVIHIINAEMENYEAGVYTEKVLEATKLLSETVMPRFAEQVEVAIEALSANVPQPFEENEFIDASRLVYDGVRDIRKAVLMIRTPEELEDDSDF----EQEDYDVRSRTSVQTE---DDQLIAGQSARAIM-----------AQLPQEEKAKIA----EQVEIF----------HQEKSKLDAEVAKWDDSGNDIIVLAKQMCMIMMEMTDFTR-GKGPLKNTSDVINAAKKIAEAGSRMDKLARAVADQCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELIVSGLDSATSLIQAAKNLMNAVVLTVKASYVASTKYQKVYG 847          

BLAST of Vinculin vs. nr
Match: gi|1325285267|ref|XP_023327348.1| (vinculin-like [Eurytemora affinis])

HSP 1 Score: 817.379 bits (2110), Expect = 0.000e+0
Identity = 451/863 (52.26%), Postives = 572/863 (66.28%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGEDGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQRNIHDPRSRQNVADLIRQLEVSVNEAVIGRIIHDMADITTPLKHFTD-----AVHSNETISMKRMS-VDEKSQTLKVFSNRLAKTANLVAFANARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPWI 857
            MP+YQT+TIA ILEPVAQQVSKLIILHEEGEDGN+MP+LE PV+AVS AV NL +VGK+TI+SSQD+K+KSEMPKSL  IEKAA+ LE A  DL  DP+SQRGRD+LI GSR ILQGTT+ML+ FD SQV+K+CRDCKKVLDYLA++EVIETMEDLVQFVKDL                       R+ LG HL+Q+KTLAPILICSMKIFIQIL QEG+GTEEAVENRNYLA+RMT+E+ E++KILE+ST+++T+ +++NG + IN  +++FHE+MK+++  +++K+    ++++ ++QHI+D+  KI++ FD   + +++Q C ++++++          RQN A  +  LE  +NEAVI RII DMA +      F++        S+   +   +  V++K   LK FS RL+KTA+LVA ANAR+KRRS SL   STQV+ LTPQL+NAGTI+M YPENKAA+ENFENLR QY  G+  IR +      T                      +  S+  LI                        ED  L+++V  A D LK  I PFV +SK +A N+SD +    W+Q A  L +MV +V +LF DLNM+G D+ R   R           PP  P    ++ N   APPRPPLP D       P     DTDDE+GLF + P  NRPIH AAHGLYQEV+QWDHTDNEIIAAAKKIA+LMAHLSEL++ G+GTKREL+ACA                                   KIPTLGTQLRVL+TVKATMM +N+++ EDQEAMDMLVFNA+KLNQAV+ETV AAE+ASIRVRSDAGFKLKW+RK  PW 
Sbjct:    1 MPLYQTRTIASILEPVAQQVSKLIILHEEGEDGNSMPNLEIPVQAVSKAVSNLVRVGKETISSSQDMKMKSEMPKSLSTIEKAANCLEEASNDLNSDPYSQRGRDKLIEGSRAILQGTTAMLISFDASQVRKLCRDCKKVLDYLAISEVIETMEDLVQFVKDL-----------------------RDGLGQHLEQMKTLAPILICSMKIFIQILNQEGKGTEEAVENRNYLARRMTEEISEVIKILEESTQTDTSARLLNG-NGINLADLSFHEIMKRVNNLVSNKT----EISRDIVQHILDMAHKIAECFDGSARTEIIQACREVERSD----------RQNTATALLNLEFRINEAVINRIIQDMAXLNDWEHMFSNHKIRIYCKSSGCYTTNNLYLVEQKGFVLKQFSGRLSKTASLVAAANARNKRRSESLVHLSTQVEHLTPQLVNAGTIKMTYPENKAADENFENLRKQYAAGVQSIRYITRGKYKTD---------------------QKYSLVTLI------------------------EDISLKKEVNQAADRLKAVIAPFVESSKLMAINMSDQNQYARWQQCASNLQEMVQQVRKLFLDLNMFGLDSERGGGRIPI-------PPPPVPPLPTSVDN--SAPPRPPLPQDVVPAPPRPPRP--DTDDEEGLFTTEPGSNRPIHMAAHGLYQEVKQWDHTDNEIIAAAKKIAYLMAHLSELIRGGKGTKRELIACA-----------------------------------KIPTLGTQLRVLSTVKATMMGQNMDSEEDQEAMDMLVFNAQKLNQAVKETVRAAEAASIRVRSDAGFKLKWVRKQ-PWF 733          

BLAST of Vinculin vs. nr
Match: gi|909554962|ref|XP_013148965.1| (PREDICTED: vinculin [Papilio polytes])

HSP 1 Score: 586.645 bits (1511), Expect = 0.000e+0
Identity = 388/988 (39.27%), Postives = 555/988 (56.17%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGEDGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKIL------EDS-----------------TRSETAHKMMNGVSSINTN--EMTFHEVMKK-------LSASLASK---------------------------------SSQDGQLNQQLIQHIVDLGFKISDG---------FDSQEKRKLLQICNDLDQANRQRNIHDPRSR-QNVADLIR---QLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVD---------EKSQTLKVFSNRLAKTANLVAFANARDK------------RRSSSLQQFST---------------------------QVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMT---MSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDE-DGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQP-GRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSD-AGFKLKWIRKPVPW 856
            MP++ TK I  ILEPVAQQVS+L+ILHEE EDGNAMPDL RPV+AVS AV+NL KVG +TI SS+D  L+ +MP +L  +E AA LL+ A   L+ DP+S   R +LI GSRGILQGT+ +L+ FDES+V+KI ++CKKVLDYL VAEVI+TMEDLVQF++D+SP LS+  +EV +R  +L +P H E+L   L+ VK LAP LIC+MKI+I IL + G+G EEA ENRNYLA+RM DE+ EI+++L      ED                  T+   AH+ +N  S++  +  E  F  V+         L+A L+                                   S   GQLN+  ++ IV+ G ++++           +S  K  L    + L +A  QR I    S+ Q +A+ ++   + EV      + R+   +AD+       +   HS E  ++ R   D         E++   +V  + +   A L  FA+A +              R+S+LQ FS                            +V+ L+PQLI AG IR++YP++K AEE+F NL+NQY + +  +RDLCD++VD   F++     +QK    CE+ +R+     +++N++  ARLANR+L+V   E DN+ED    R +  A   L+ +I P V  +K +A  +SD + V  WR     ++   +EV                        E  +    PA  LA +    +S+  P  P PP P  A     PP P   DTDDE + +F   P  ++PI  AAH L++ VR+W   DNEIIAAAK++A LMA LS+LV+   +G+KREL+A A+ +AE SE +T LAK+    CTDK+IRTNLLQVCE+IPT+GTQL++L+TVKATM+     + EDQEA +MLV NA+ L Q+V+ETV A+E ASI++R++  G++L+W+R+  PW
Sbjct:    1 MPVFHTKIIESILEPVAQQVSRLVILHEEAEDGNAMPDLARPVQAVSLAVNNLVKVGHETIESSEDAILRQDMPGALRRVEGAATLLQQASDMLRADPYSGPARKKLIEGSRGILQGTSGLLLCFDESEVRKIVKECKKVLDYLGVAEVIDTMEDLVQFLRDISPALSRAAREVASRAAELTHPPHAETLARCLESVKQLAPHLICAMKIYIHILTEGGKGMEEAAENRNYLAQRMGDEIHEIIRVLQLTSYIEDGGEKDNVALLKALQTQIHTKMGPAHEFLNDSSALRNSVPERAFRSVLTSAQRAADHLAADLSDSLRRAVRSGGINADHLCDERQLGRGRDSKALNLASELRGQLNE--VEGIVNEGVRVAEKQGGKTLQSRLESAHKWLLQPAADALIRAEGQRAIASVVSQGQRIAEGLQGREKAEVLQQCGDVQRLADKLADLCA-----SGQGHSEEARAITRELSDKLHELKRCMERAVVNRVVEDFIDVAAPLRHFADAVNAPEGSPNREANFLERASALQAFSARAAAAAAVVAAAAHDKRLADQLLHNKREVEKLSPQLIAAGKIRLHYPDSKVAEEHFNNLKNQYADAVLRVRDLCDQAVDPLDFVRTAGELMQKHTYLCEDAIRNNDAQKMVDNTSAIARLANRVLLVGGAECDNTEDGEFGRALAAAQQRLQASIAPAVRLAKRVA--LSDRAAVPAWRSANTEIMSAASEV------------------------EAALSRHAPADTLAALGALRLSDTPPPRPPPPAPAPAPHGPAPPRPPPPDTDDEGEDIFRRQPHPSQPILVAAHNLHRAVREWSSKDNEIIAAAKRMAILMARLSDLVRSDSKGSKRELIATAKAIAEASEEVTRLAKKLALECTDKRIRTNLLQVCERIPTIGTQLKILSTVKATMLGAQ-GSEEDQEATEMLVGNAQNLMQSVKETVKASEGASIKIRTEQGGYRLRWVRRS-PW 953          

BLAST of Vinculin vs. nr
Match: gi|1240376185|gb|PAV86844.1| (hypothetical protein WR25_24234 [Diploscapter pachys])

HSP 1 Score: 542.732 bits (1397), Expect = 5.977e-173
Identity = 347/960 (36.15%), Postives = 522/960 (54.37%), Query Frame = 0

Query:   20 VSKLIILHEEGEDGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEG-RGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSE---------TAHKMMNGVSSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKIS------DGFDSQEKRK---LLQICNDLDQANRQRNIHDPRS----------RQNVADLIRQLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFAN-ARDKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKG--------------------------------------------AITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIP-----------IEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAH--------GLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMM---SENVE-------------------------TPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPWIN 858
            VS+L+ILHEE EDGNAMPDL RPV  VS AV+NL KVG DT +SS D  L+++MP +L  +E ++ LLE AC  LK DPFS   R +LI G+RGILQGT+++L+ FDES+V+KI R C+KVLDYLAVAEVIE M+DL QFVKD+SP L++V  +V+ R+++L +  HRE L   LD +KTL+PILIC+MKIFIQI      RG  EA ENRNYLA+RMTDEM EI+++L+ +T  E            K ++   S+ T  + +          LA    + G + ++ I+ I++   +I+      D +  + + K   LL +CND+D+   Q    + R           R  + D +R+L   + + +  R++ D ADITTPLK F +AVH++  +  +  +  EK+  L   S  + +TA LVA     + K+   ++   + ++  +TPQ +NAG IR+    ++ AE +FENL  QY + +H +R   D+++DT  F++ ++  +++    CE  + S     +++N++  ARL NR+LM    EADNSE+P    +V  A   L                                               I P V ++K +A N  D     NWR     L+D V  V      L   G  + ++   +     P           +  TPPA  + +  M  +I      P      +   PP       + ++     A     P+  A+H        GL+ E++QW   +N+I+AAAK++A LMA LS+LV+   GTK++L+ C++ +A+ SE +T LA    + CTD K+R  LLQVCE+IPT+ TQL++L+TVKATM+   S+++E                         + ED+EAM  LV NA+ L Q+V++TV AAE+ASI++R+++G +L+W+RKP+ W N
Sbjct:  275 VSRLVILHEEAEDGNAMPDLTRPVGLVSRAVENLIKVGYDTCDSSDDRVLQADMPPALQRVEGSSKLLEDACHMLKGDPFSVPARKKLIDGARGILQGTSALLLCFDESEVRKIIRGCRKVLDYLAVAEVIEGMDDLAQFVKDISPWLTRVTTDVNLRQQELTHQVHREILIRCLDAIKTLSPILICAMKIFIQINNDPSQRGGAEAAENRNYLAQRMTDEMNEIIRVLQLTTYDEDEWDADNVTVMRKALSAAKSLLTAALDW----------LADPRGRPGAVGEKAIRRILEYSERIAERALPEDSYSIKVRLKVNRLLGLCNDIDRMVGQLYDLERRGLGNTPEAQALRAQLKDRLRELADFMKKILTDRVVDDFADITTPLKQFVEAVHADPYMPNREQNFVEKANKLTDHSRSMTQTARLVATCGPCKSKKTIEAIVDTAEKIDQMTPQFVNAGKIRL-LNSSEPAEVHFENLHRQYADALHRLRSHVDDAIDTGEFVRASENAMRRYTTHCESAITSNHPQGMVDNTSQIARLGNRVLMTAKNEADNSEEPAFVDRVNTAASQLHSVTRMPPPQRYSSSEVSSDPYESDEYDDEETYYYRTERFNPQVYITIPPMVNHAKQVALNPRDTHASDNWRGANDHLLDSVRAVGDAITGLPANGRSSYQESYSRGSNYTPQPRESSQVLRTVAATPPAPAVVHNKM--IIREEIPAPPRPPPPVELSPPPRPPPPPEYDEEEETRAFWERYPLPQASHQPILSAAYGLHNELKQWSSQENDIVAAAKRMAILMARLSQLVRGEGGTKKDLIDCSKAIADSSEEVTRLAVHIARLCTDIKMRMALLQVCERIPTIATQLKILSTVKATMLGSSSKSLEDGRRKTEPFCPSQMTDSAISAHSSGSEEDEEAMQQLVLNAQNLMQSVKDTVRAAEAASIKIRTNSGLRLRWLRKPM-WSN 1220          

BLAST of Vinculin vs. nr
Match: gi|1228001167|ref|XP_021932459.1| (vinculin isoform X6 [Zootermopsis nevadensis])

HSP 1 Score: 462.611 bits (1189), Expect = 7.574e-146
Identity = 273/671 (40.69%), Postives = 403/671 (60.06%), Query Frame = 0

Query:  215 LGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGV--SSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQ------RNIHDPRSRQNVADLIRQ----LEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANAR-DKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITP--PAKP-LANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQ-PGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETP------------EDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            L Q+G+G   A      LA  + +++GE+  +++         + + GV  S +     T    +++    LA     D  L QQ I  IV  G K++DG     K ++L +C+++D  +RQ      R   D    Q+VA  + Q    L+  +  AV+ R++ D  DI++PLKHFTDAVH  +    +  + ++++Q L+ FS+R AKTA +VA   +  +K+ + +L   ++QV++LTPQL++AG IRMNYPE+KAA+E+F+NL  QY++ +  +R LCDE+ D+  F++ ++ QIQK    CEE +R      +++N+   ARLANR+LMV  +E+DNSEDP    ++  A D ++ ++TP V ++KA+A N  D   V  WR+  + L+  V EV                           + ITP  P+ P ++N+ ++    APPRPPLP     P R   P   +TDDED +FL AP  N+PI  AAHGL+QEVRQW   DN+II AAKK+A LM  LS+LV+  G  +KR+L++CA+ +AE SE +T LAKE  + CTDK++RTNLLQVCE+IPT+GTQL++L+TVKATM+      P            EDQEA DMLV NA+ L Q+V+ETV AAESASI++R+DAG +L+W+R+  PW
Sbjct:  265 LRQDGKG---ATPQAECLAHGIQEKLGELCGLVQ---------RAVVGVEKSGVQQPAHTVSGRLEQARRWLAHPDRDDRGLGQQAIALIVTEGKKVADGLPGIHKAEILGLCDEVDILSRQLSDLCRRGHGDTPQAQDVARSLSQKLYELKDRIQNAVVSRVVEDFIDISSPLKHFTDAVHVPQGTPGREQNFNDRAQQLQQFSSRAAKTARMVAAGGSSGNKKLAEALLSSASQVESLTPQLVSAGRIRMNYPESKAADEHFQNLVAQYSDSVQHVRALCDEATDSADFIKMSEEQIQKHSILCEEAIRKIQPQKMVDNTASIARLANRVLMVAKQESDNSEDPQFISRINSASDQVQASVTPMVQDAKAVAVNTQDSVAVSRWRESNRALLTAVGEVRH------------------------AVTITPDLPSPPDVSNLHINEY--APPRPPLPGGEVPPPR---PPPPETDDEDDMFLHAPLPNQPIMMAAHGLHQEVRQWSSKDNDIIVAAKKMAVLMGRLSQLVRGEGGSSKRDLISCAKSIAEASEEVTRLAKELARECTDKRMRTNLLQVCERIPTIGTQLKILSTVKATMLGAQEVIPRMDMGEILGGTEEDQEATDMLVGNAQNLMQSVKETVRAAESASIKIRTDAGIRLRWVRRQ-PW 893          

HSP 2 Score: 241.891 bits (616), Expect = 6.848e-64
Identity = 119/182 (65.38%), Postives = 146/182 (80.22%), Query Frame = 0

Query:   73 MPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSE 254
            MP +LV +E A+ LLE A   LK DP+S   R +LI GSRGILQGT+++L+ FDES+V+KI R+CK+VLDYLAVAEVIETMEDLVQF+KDLSPCLSKV +EV  R K+L +  HRE L   L+QVKTLAPILICSMKIFI I+ Q G+G EEA ENRNYLA+RMTDE+ EI+++L+ +T  E
Sbjct:    1 MPAALVRVEGASRLLEEASAMLKVDPYSGPARKKLIEGSRGILQGTSALLLCFDESEVRKIIRECKRVLDYLAVAEVIETMEDLVQFLKDLSPCLSKVSREVGGREKELTHQVHREILVRCLEQVKTLAPILICSMKIFIHIIAQGGKGAEEAAENRNYLAQRMTDELNEIIRVLQLTTYDE 182          

BLAST of Vinculin vs. nr
Match: gi|646704590|gb|KDR12694.1| (Vinculin [Zootermopsis nevadensis])

HSP 1 Score: 461.455 bits (1186), Expect = 2.897e-145
Identity = 276/679 (40.65%), Postives = 406/679 (59.79%), Query Frame = 0

Query:  205 ICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGV--SSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQ------RNIHDPRSRQNVADLIRQ----LEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANAR-DKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLF----------DDLNMYGFDAP---RDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQ-PGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            I +M   +  L Q+G+G   A      LA  + +++GE+  +++         + + GV  S +     T    +++    LA     D  L QQ I  IV  G K++DG     K ++L +C+++D  +RQ      R   D    Q+VA  + Q    L+  +  AV+ R++ D  DI++PLKHFTDAVH  +    +  + ++++Q L+ FS+R AKTA +VA   +  +K+ + +L   ++QV++LTPQL++AG IRMNYPE+KAA+E+F+NL  QY++ +  +R LCDE+ D+  F++ ++ QIQK    CEE +R      +++N+   ARLANR+LMV  +E+DNSEDP    ++  A D ++ ++TP V ++KA+A N  D   V  WR+  + L+  V EV              D  N++  D     + YQ   QQ                  ++   APPRPPLP     P R   P   +TDDED +FL AP  N+PI  AAHGL+QEVRQW   DN+II AAKK+A LM  LS+LV+  G  +KR+L++CA+ +AE SE +T LAKE  + CTDK++RTNLLQVCE+IPT+GTQL++L+TVKATM+     T EDQEA DMLV NA+ L Q+V+ETV AAESASI++R+DAG +L+W+R+  PW
Sbjct:  255 ITTMTDALCELRQDGKG---ATPQAECLAHGIQEKLGELCGLVQ---------RAVVGVEKSGVQQPAHTVSGRLEQARRWLAHPDRDDRGLGQQAIALIVTEGKKVADGLPGIHKAEILGLCDEVDILSRQLSDLCRRGHGDTPQAQDVARSLSQKLYELKDRIQNAVVSRVVEDFIDISSPLKHFTDAVHVPQGTPGREQNFNDRAQQLQQFSSRAAKTARMVAAGGSSGNKKLAEALLSSASQVESLTPQLVSAGRIRMNYPESKAADEHFQNLVAQYSDSVQHVRALCDEATDSADFIKMSEEQIQKHSILCEEAIRKIQPQKMVDNTASIARLANRVLMVAKQESDNSEDPQFISRINSASDQVQASVTPMVQDAKAVAVNTQDSVAVSRWRESNRALLTAVGEVRHAVTITPDLPSPPDVSNLHINDKSHLVQGYQPSQQQ--------------YNYFTDKEYAPPRPPLPGGEVPPPR---PPPPETDDEDDMFLHAPLPNQPIMMAAHGLHQEVRQWSSKDNDIIVAAKKMAVLMGRLSQLVRGEGGSSKRDLISCAKSIAEASEEVTRLAKELARECTDKRMRTNLLQVCERIPTIGTQLKILSTVKATMLGAQ-GTEEDQEATDMLVGNAQNLMQSVKETVRAAESASIKIRTDAGIRLRWVRRQ-PW 902          

HSP 2 Score: 242.276 bits (617), Expect = 6.080e-64
Identity = 119/182 (65.38%), Postives = 146/182 (80.22%), Query Frame = 0

Query:   73 MPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSE 254
            MP +LV +E A+ LLE A   LK DP+S   R +LI GSRGILQGT+++L+ FDES+V+KI R+CK+VLDYLAVAEVIETMEDLVQF+KDLSPCLSKV +EV  R K+L +  HRE L   L+QVKTLAPILICSMKIFI I+ Q G+G EEA ENRNYLA+RMTDE+ EI+++L+ +T  E
Sbjct:    1 MPAALVRVEGASRLLEEASAMLKVDPYSGPARKKLIEGSRGILQGTSALLLCFDESEVRKIIRECKRVLDYLAVAEVIETMEDLVQFLKDLSPCLSKVSREVGGREKELTHQVHREILVRCLEQVKTLAPILICSMKIFIHIIAQGGKGAEEAAENRNYLAQRMTDELNEIIRVLQLTTYDE 182          

BLAST of Vinculin vs. nr
Match: gi|321460281|gb|EFX71325.1| (hypothetical protein DAPPUDRAFT_308937 [Daphnia pulex])

HSP 1 Score: 461.84 bits (1187), Expect = 6.425e-145
Identity = 263/599 (43.91%), Postives = 375/599 (62.60%), Query Frame = 0

Query:  274 HEVMKKLSAS---LASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQRN-------IHDPRSRQNVADLIRQ----LEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANAR-DKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNL-IPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQPGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            H V  +L  +   LA+ S  D  L QQ I  IV+ G K++ G   +++ ++L +CN +D  +RQ          + P++ Q VA L+ Q    L+  +  A++ R++ D  DITTPLK FTDAV + E    +  +  +K+  LK FS R A+TA +VA   +  +K+ S +L   + QV++LTPQL+NAG IRM YPENKAA+E+FENLR QY + I   R LCDE++D+  F+Q ++  +++    CE+ +  +    L++ ++  ARLANR+LMV  +EADNSEDP    +V GA + L+  +TP V ++KA+ATN+ D   +  WR   ++L++ V +V +                           +  P        MS+L + APPRPPLP     P RPP P   +TDDED +F  AP G++PI  AAHGL+QEV QW   DNEIIAAAK++A LM  LS+LV+   G+KR+L+ACA+ +AE SE +T LAKE  + CTDK++RT LLQVCE+IPT+GTQL++L+TVKATM+     T EDQEA DMLV NA+ L Q+V+ETV AAE+ASI++R+DAG ++ WIR+  PW
Sbjct:  383 HTVAGRLEQARRWLANPSVDDRGLGQQAIALIVEEGRKVAQGLQGEQRAEILALCNQVDSLSRQLGDLCRSGQGNSPQA-QAVAKLLSQKLDELKDKIQGALVDRVVEDFIDITTPLKQFTDAVLAPEGTPNREENFKDKAGALKGFSARAAQTARMVAAGGSNGNKKLSEALNSSAGQVESLTPQLVNAGRIRMTYPENKAADEHFENLRRQYADAIARTRQLCDEAIDSATFIQQSEELMRRCTAECEDAIGRQEAQRLVDGTSQIARLANRVLMVAKQEADNSEDPKFIGEVTGAANLLQANVTPMVQDAKAVATNMRDQGSINQWRDSNKKLLESVGKVRK--------------------------AVAGPDPVPPPPDMSSLNLNAPPRPPLPGGEHPPPRPPPP---ETDDEDEMFRHAPQGDQPILMAAHGLHQEVMQWSSRDNEIIAAAKRMALLMGRLSQLVRGEGGSKRDLIACAKAIAEASEEVTRLAKELARECTDKRMRTALLQVCERIPTIGTQLKILSTVKATMLGAQ-GTEEDQEATDMLVGNAQNLMQSVKETVRAAEAASIKLRTDAGIRMHWIRRN-PW 949          

HSP 2 Score: 347.051 bits (889), Expect = 1.488e-101
Identity = 182/339 (53.69%), Postives = 243/339 (71.68%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGEDGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGVSSINTNEMTFHEVMKKLSASLASK-----------SSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDL 328
            MP++ TKTI  ILEPVA QV +L+ILHEE EDGNAMPDLERPV AVS AV NL KVGK+TI+SS D  LK +MP SL  +E A+ LLE A   LK DP+S   R +LI GSRGILQGT+S+L+ FDES+V+KI R+CKKVLDYLAVAEVIE+++DLVQFVKD+SPCL++V +EVD R+K+L +  HR+ L   L+QVKTLAPILICSMKIFIQI+ Q G+G EEA ENRNYLA RMTDE+ EI+++L+ +T  E            + +++T    MK+   +L SK            ++ G + ++ I+ I++L  K+++     +++ L ++C++L
Sbjct:    1 MPVFHTKTIESILEPVASQVERLVILHEEAEDGNAMPDLERPVHAVSVAVTNLVKVGKETIHSSDDAILKQDMPASLHRVEGASRLLEEASSMLKADPYSGPARKKLIEGSRGILQGTSSLLLCFDESEVRKIIRECKKVLDYLAVAEVIESIDDLVQFVKDVSPCLTRVSREVDARQKELTHQVHRDILIRSLEQVKTLAPILICSMKIFIQIVSQGGKGNEEAAENRNYLAGRMTDEINEIIRVLQLTTYDE---------DEWDADQLT---AMKRACNALESKLQTAIDWLEDPHARGGGVGEKSIRQIIELAEKVAEHSLPDDQKALRKLCSEL 327          

BLAST of Vinculin vs. nr
Match: gi|1330931642|gb|PNF42368.1| (Vinculin [Cryptotermes secundus])

HSP 1 Score: 460.299 bits (1183), Expect = 2.186e-144
Identity = 271/656 (41.31%), Postives = 395/656 (60.21%), Query Frame = 0

Query:  215 LGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGV--SSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQ------RNIHDPRSRQNVADLIRQ----LEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANAR-DKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQ-PGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            L Q+G+G   A      LA  + +++GE+  +++         + + GV  S +     T    +++    LA     D  L QQ I  IV  G K++DG     K ++L +C+++D  +RQ      R   D    Q+VA  + Q    L+  +  AV+ R++ D  DI++PLKHFTDAVH  +    +  + ++++Q L+ FS+R AKTA +VA   +  +K+ + +L   ++QV++LTPQL++AG IRMNYPE+KAA+E+F+NL  QY++ I  +R LCDE+ D+  F++ ++ QIQK    CEE +R      +++N+   ARLANR+LMV  +E+DNSEDP    +V  A D ++ ++TP V ++KA+A N  D   V  WR+  + L+  V EV                           + +TP   P     MSNL  +   PP P        PP P   +TDDED +FL AP  N+PI  AAHGL+QEVRQW   DN+II AAKK+A LM  LS+LV+  G  +KR+L++CA+ +AE SE +T LAKE  + CTDK++RTNLLQVCE+IPT+GTQL++L+TVKATM+     T EDQEA DMLV NA+ L Q+V+ETV AAESASI++R+DAG +L+W+R+  PW
Sbjct:  337 LRQDGKG---ATPQAECLAHGIQEKLGELCGLVQ---------RAVIGVEKSGVQQPAHTVSGRLEQARRWLAHPDRDDRGLGQQAIALIVTEGKKVADGLPGIHKAEILGLCDEVDILSRQLSDLCRRGHGDTPQAQDVARTLSQKLYELKDRIQNAVVSRVVEDFIDISSPLKHFTDAVHVPQGTPGREQNFNDRAQQLQQFSSRAAKTARMVAAGGSSGNKKLAEALLSSASQVESLTPQLVSAGRIRMNYPESKAADEHFQNLVAQYSDSIQHVRALCDEATDSSDFIKMSEEQIQKHSVLCEEAIRKTQPQKMVDNTASIARLANRVLMVAKQESDNSEDPQFISRVNNASDQVQASVTPMVQDAKAVAINTQDTVAVSRWRESNKALLSAVGEVRH------------------------AVTVTPDLPPPPE--MSNLHISEYAPPRPPLPGGELPPPRPPPPETDDEDDMFLHAPLPNQPIMMAAHGLHQEVRQWSSKDNDIIVAAKKMAVLMGRLSQLVRGEGGSSKRDLISCAKSIAEASEEVTRLAKELARECTDKRMRTNLLQVCERIPTIGTQLKILSTVKATMLGAQ-GTEEDQEATDMLVGNAQNLMQSVKETVRAAESASIKIRTDAGIRLRWVRRQ-PW 952          

HSP 2 Score: 348.977 bits (894), Expect = 2.497e-102
Identity = 171/254 (67.32%), Postives = 207/254 (81.50%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGEDGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSE 254
            MP++ TKTI  ILEPVAQQVS+L+ILHEE EDGNAMPDLERPV AVS AV NL KVG++TINSS+D  LK +MP +L+ +E A+ LLE A   LK DP+S   R +LI GSRGILQGT+++L+ FDES+V+KI R+CK+VLDYLAVAEVIETMEDLVQF+KDLSPCLSKV +EV  R K+L +  HRE L   L+QVKTLAPILICSMKIFI I+ Q G+G EEA ENRNYLA+RMTDE+ EI+++L+ +T  E
Sbjct:    1 MPVFHTKTIESILEPVAQQVSRLVILHEEAEDGNAMPDLERPVMAVSRAVTNLVKVGRETINSSEDAILKQDMPAALIRVEGASRLLEEASAMLKVDPYSGPARKKLIEGSRGILQGTSALLLCFDESEVRKIIRECKRVLDYLAVAEVIETMEDLVQFLKDLSPCLSKVSREVGGREKELTHQVHREILVRCLEQVKTLAPILICSMKIFIHIIAQGGKGAEEAAENRNYLAQRMTDELNEIIRVLQLTTYDE 254          

BLAST of Vinculin vs. nr
Match: gi|1228001165|ref|XP_021932457.1| (vinculin isoform X5 [Zootermopsis nevadensis])

HSP 1 Score: 456.833 bits (1174), Expect = 1.872e-143
Identity = 276/691 (39.94%), Postives = 406/691 (58.76%), Query Frame = 0

Query:  205 ICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGV--SSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQ------RNIHDPRSRQNVADLIRQ----LEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANAR-DKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLF----------DDLNMYGFDAP---RDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQ-PGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETP------------EDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            I +M   +  L Q+G+G   A      LA  + +++GE+  +++         + + GV  S +     T    +++    LA     D  L QQ I  IV  G K++DG     K ++L +C+++D  +RQ      R   D    Q+VA  + Q    L+  +  AV+ R++ D  DI++PLKHFTDAVH  +    +  + ++++Q L+ FS+R AKTA +VA   +  +K+ + +L   ++QV++LTPQL++AG IRMNYPE+KAA+E+F+NL  QY++ +  +R LCDE+ D+  F++ ++ QIQK    CEE +R      +++N+   ARLANR+LMV  +E+DNSEDP    ++  A D ++ ++TP V ++KA+A N  D   V  WR+  + L+  V EV              D  N++  D     + YQ   QQ                  ++   APPRPPLP     P R   P   +TDDED +FL AP  N+PI  AAHGL+QEVRQW   DN+II AAKK+A LM  LS+LV+  G  +KR+L++CA+ +AE SE +T LAKE  + CTDK++RTNLLQVCE+IPT+GTQL++L+TVKATM+      P            EDQEA DMLV NA+ L Q+V+ETV AAESASI++R+DAG +L+W+R+  PW
Sbjct:  255 ITTMTDALCELRQDGKG---ATPQAECLAHGIQEKLGELCGLVQ---------RAVVGVEKSGVQQPAHTVSGRLEQARRWLAHPDRDDRGLGQQAIALIVTEGKKVADGLPGIHKAEILGLCDEVDILSRQLSDLCRRGHGDTPQAQDVARSLSQKLYELKDRIQNAVVSRVVEDFIDISSPLKHFTDAVHVPQGTPGREQNFNDRAQQLQQFSSRAAKTARMVAAGGSSGNKKLAEALLSSASQVESLTPQLVSAGRIRMNYPESKAADEHFQNLVAQYSDSVQHVRALCDEATDSADFIKMSEEQIQKHSILCEEAIRKIQPQKMVDNTASIARLANRVLMVAKQESDNSEDPQFISRINSASDQVQASVTPMVQDAKAVAVNTQDSVAVSRWRESNRALLTAVGEVRHAVTITPDLPSPPDVSNLHINDKSHLVQGYQPSQQQ--------------YNYFTDKEYAPPRPPLPGGEVPPPR---PPPPETDDEDDMFLHAPLPNQPIMMAAHGLHQEVRQWSSKDNDIIVAAKKMAVLMGRLSQLVRGEGGSSKRDLISCAKSIAEASEEVTRLAKELARECTDKRMRTNLLQVCERIPTIGTQLKILSTVKATMLGAQEVIPRMDMGEILGGTEEDQEATDMLVGNAQNLMQSVKETVRAAESASIKIRTDAGIRLRWVRRQ-PW 915          

HSP 2 Score: 241.891 bits (616), Expect = 7.171e-64
Identity = 119/182 (65.38%), Postives = 146/182 (80.22%), Query Frame = 0

Query:   73 MPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSE 254
            MP +LV +E A+ LLE A   LK DP+S   R +LI GSRGILQGT+++L+ FDES+V+KI R+CK+VLDYLAVAEVIETMEDLVQF+KDLSPCLSKV +EV  R K+L +  HRE L   L+QVKTLAPILICSMKIFI I+ Q G+G EEA ENRNYLA+RMTDE+ EI+++L+ +T  E
Sbjct:    1 MPAALVRVEGASRLLEEASAMLKVDPYSGPARKKLIEGSRGILQGTSALLLCFDESEVRKIIRECKRVLDYLAVAEVIETMEDLVQFLKDLSPCLSKVSREVGGREKELTHQVHREILVRCLEQVKTLAPILICSMKIFIHIIAQGGKGAEEAAENRNYLAQRMTDELNEIIRVLQLTTYDE 182          

BLAST of Vinculin vs. nr
Match: gi|1339064451|ref|XP_023714733.1| (vinculin isoform X4 [Cryptotermes secundus])

HSP 1 Score: 457.988 bits (1177), Expect = 2.260e-143
Identity = 271/663 (40.87%), Postives = 395/663 (59.58%), Query Frame = 0

Query:  215 LGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGV--SSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQ------RNIHDPRSRQNVADLIR-----------QLEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANAR-DKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEVARLFDDLNMYGFDAPRDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQ-PGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETPEDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            L Q+G+G   A      LA  + +++GE+  +++         + + GV  S +     T    +++    LA     D  L QQ I  IV  G K++DG     K ++L +C+++D  +RQ      R   D    Q+VA  I            +L+  +  AV+ R++ D  DI++PLKHFTDAVH  +    +  + ++++Q L+ FS+R AKTA +VA   +  +K+ + +L   ++QV++LTPQL++AG IRMNYPE+KAA+E+F+NL  QY++ I  +R LCDE+ D+  F++ ++ QIQK    CEE +R      +++N+   ARLANR+LMV  +E+DNSEDP    +V  A D ++ ++TP V ++KA+A N  D   V  WR+  + L+  V EV                           + +TP   P     MSNL  +   PP P        PP P   +TDDED +FL AP  N+PI  AAHGL+QEVRQW   DN+II AAKK+A LM  LS+LV+  G  +KR+L++CA+ +AE SE +T LAKE  + CTDK++RTNLLQVCE+IPT+GTQL++L+TVKATM+     T EDQEA DMLV NA+ L Q+V+ETV AAESASI++R+DAG +L+W+R+  PW
Sbjct:  337 LRQDGKG---ATPQAECLAHGIQEKLGELCGLVQ---------RAVIGVEKSGVQQPAHTVSGRLEQARRWLAHPDRDDRGLGQQAIALIVTEGKKVADGLPGIHKAEILGLCDEVDILSRQLSDLCRRGHGDTPQAQDVASTINHVARTLSQKLYELKDRIQNAVVSRVVEDFIDISSPLKHFTDAVHVPQGTPGREQNFNDRAQQLQQFSSRAAKTARMVAAGGSSGNKKLAEALLSSASQVESLTPQLVSAGRIRMNYPESKAADEHFQNLVAQYSDSIQHVRALCDEATDSSDFIKMSEEQIQKHSVLCEEAIRKTQPQKMVDNTASIARLANRVLMVAKQESDNSEDPQFISRVNNASDQVQASVTPMVQDAKAVAINTQDTVAVSRWRESNKALLSAVGEVRH------------------------AVTVTPDLPPPPE--MSNLHISEYAPPRPPLPGGELPPPRPPPPETDDEDDMFLHAPLPNQPIMMAAHGLHQEVRQWSSKDNDIIVAAKKMAVLMGRLSQLVRGEGGSSKRDLISCAKSIAEASEEVTRLAKELARECTDKRMRTNLLQVCERIPTIGTQLKILSTVKATMLGAQ-GTEEDQEATDMLVGNAQNLMQSVKETVRAAESASIKIRTDAGIRLRWVRRQ-PW 959          

HSP 2 Score: 348.977 bits (894), Expect = 2.608e-102
Identity = 171/254 (67.32%), Postives = 207/254 (81.50%), Query Frame = 0

Query:    1 MPMYQTKTIAGILEPVAQQVSKLIILHEEGEDGNAMPDLERPVKAVSHAVDNLAKVGKDTINSSQDIKLKSEMPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSE 254
            MP++ TKTI  ILEPVAQQVS+L+ILHEE EDGNAMPDLERPV AVS AV NL KVG++TINSS+D  LK +MP +L+ +E A+ LLE A   LK DP+S   R +LI GSRGILQGT+++L+ FDES+V+KI R+CK+VLDYLAVAEVIETMEDLVQF+KDLSPCLSKV +EV  R K+L +  HRE L   L+QVKTLAPILICSMKIFI I+ Q G+G EEA ENRNYLA+RMTDE+ EI+++L+ +T  E
Sbjct:    1 MPVFHTKTIESILEPVAQQVSRLVILHEEAEDGNAMPDLERPVMAVSRAVTNLVKVGRETINSSEDAILKQDMPAALIRVEGASRLLEEASAMLKVDPYSGPARKKLIEGSRGILQGTSALLLCFDESEVRKIIRECKRVLDYLAVAEVIETMEDLVQFLKDLSPCLSKVSREVGGREKELTHQVHREILVRCLEQVKTLAPILICSMKIFIHIIAQGGKGAEEAAENRNYLAQRMTDELNEIIRVLQLTTYDE 254          

BLAST of Vinculin vs. nr
Match: gi|1228001163|ref|XP_021932456.1| (vinculin isoform X4 [Zootermopsis nevadensis])

HSP 1 Score: 456.447 bits (1173), Expect = 3.957e-143
Identity = 277/699 (39.63%), Postives = 405/699 (57.94%), Query Frame = 0

Query:  215 LGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSETAHKMMNGV--SSINTNEMTFHEVMKKLSASLASKSSQDGQLNQQLIQHIVDLGFKISDGFDSQEKRKLLQICNDLDQANRQ------RNIHDPRSRQNVADLIRQ----LEVSVNEAVIGRIIHDMADITTPLKHFTDAVHSNETISMKRMSVDEKSQTLKVFSNRLAKTANLVAFANAR-DKRRSSSLQQFSTQVQTLTPQLINAGTIRMNYPENKAAEENFENLRNQYNEGIHLIRDLCDESVDTKIFLQHTQAQIQKAIQGCEEGLRSRSVHILIENSTLAARLANRLLMVLHKEADNSEDPGLRRQVIGAGDHLKGAITPFVTNSKALATNVSDGSLVKNWRQGAQRLVDMVNEV-----------------------ARLFDDLN-----MYGFDAP---RDYQRQTQQEIPIEITPPAKPLANMTMSNLIPAPPRPPLPTDARLPKRPPGPRGCDTDDEDGLFLSAPAGNRPIHHAAHGLYQEVRQWDHTDNEIIAAAKKIAFLMAHLSELVQ-PGRGTKRELLACARGLAEVSERITELAKEYGKHCTDKKIRTNLLQVCEKIPTLGTQLRVLTTVKATMMSENVETP------------EDQEAMDMLVFNAEKLNQAVRETVGAAESASIRVRSDAGFKLKWIRKPVPW 856
            L Q+G+G   A      LA  + +++GE+  +++         + + GV  S +     T    +++    LA     D  L QQ I  IV  G K++DG     K ++L +C+++D  +RQ      R   D    Q+VA  + Q    L+  +  AV+ R++ D  DI++PLKHFTDAVH  +    +  + ++++Q L+ FS+R AKTA +VA   +  +K+ + +L   ++QV++LTPQL++AG IRMNYPE+KAA+E+F+NL  QY++ +  +R LCDE+ D+  F++ ++ QIQK    CEE +R      +++N+   ARLANR+LMV  +E+DNSEDP    ++  A D ++ ++TP V ++KA+A N  D   V  WR+  + L+  V EV                       A L D  N     ++G D     + YQ   QQ                  ++   APPRPPLP     P R   P   +TDDED +FL AP  N+PI  AAHGL+QEVRQW   DN+II AAKK+A LM  LS+LV+  G  +KR+L++CA+ +AE SE +T LAKE  + CTDK++RTNLLQVCE+IPT+GTQL++L+TVKATM+      P            EDQEA DMLV NA+ L Q+V+ETV AAESASI++R+DAG +L+W+R+  PW
Sbjct:  265 LRQDGKG---ATPQAECLAHGIQEKLGELCGLVQ---------RAVVGVEKSGVQQPAHTVSGRLEQARRWLAHPDRDDRGLGQQAIALIVTEGKKVADGLPGIHKAEILGLCDEVDILSRQLSDLCRRGHGDTPQAQDVARSLSQKLYELKDRIQNAVVSRVVEDFIDISSPLKHFTDAVHVPQGTPGREQNFNDRAQQLQQFSSRAAKTARMVAAGGSSGNKKLAEALLSSASQVESLTPQLVSAGRIRMNYPESKAADEHFQNLVAQYSDSVQHVRALCDEATDSADFIKMSEEQIQKHSILCEEAIRKIQPQKMVDNTASIARLANRVLMVAKQESDNSEDPQFISRINSASDQVQASVTPMVQDAKAVAVNTQDSVAVSRWRESNRALLTAVGEVRHAVTITPDLPSPPDVSNLHINDAELGDIFNVDFNSIFGLDKSHLVQGYQPSQQQ--------------YNYFTDKEYAPPRPPLPGGEVPPPR---PPPPETDDEDDMFLHAPLPNQPIMMAAHGLHQEVRQWSSKDNDIIVAAKKMAVLMGRLSQLVRGEGGSSKRDLISCAKSIAEASEEVTRLAKELARECTDKRMRTNLLQVCERIPTIGTQLKILSTVKATMLGAQEVIPRMDMGEILGGTEEDQEATDMLVGNAQNLMQSVKETVRAAESASIKIRTDAGIRLRWVRRQ-PW 933          

HSP 2 Score: 241.891 bits (616), Expect = 1.086e-63
Identity = 119/182 (65.38%), Postives = 146/182 (80.22%), Query Frame = 0

Query:   73 MPKSLVAIEKAADLLEMACGDLKQDPFSQRGRDQLIAGSRGILQGTTSMLVVFDESQVKKICRDCKKVLDYLAVAEVIETMEDLVQFVKDLSPCLSKVCQEVDTRRKDLLNPYHRESLGLHLDQVKTLAPILICSMKIFIQILGQEGRGTEEAVENRNYLAKRMTDEMGEIVKILEDSTRSE 254
            MP +LV +E A+ LLE A   LK DP+S   R +LI GSRGILQGT+++L+ FDES+V+KI R+CK+VLDYLAVAEVIETMEDLVQF+KDLSPCLSKV +EV  R K+L +  HRE L   L+QVKTLAPILICSMKIFI I+ Q G+G EEA ENRNYLA+RMTDE+ EI+++L+ +T  E
Sbjct:    1 MPAALVRVEGASRLLEEASAMLKVDPYSGPARKKLIEGSRGILQGTSALLLCFDESEVRKIIRECKRVLDYLAVAEVIETMEDLVQFLKDLSPCLSKVSREVGGREKELTHQVHREILVRCLEQVKTLAPILICSMKIFIHIIAQGGKGAEEAAENRNYLAQRMTDELNEIIRVLQLTTYDE 182