BLAST of af512518_1pyrroline-5-carboxylase synthase vs. L. salmonis genes
Match: EMLSAG00000012086 (supercontig:LSalAtl2s:LSalAtl2s864:16880:21044:-1 gene:EMLSAG00000012086 transcript:EMLSAT00000012086 description:"maker-LSalAtl2s864-augustus-gene-0.16")

HSP 1 Score: 1021.92 bits (2641), Expect = 0.000e+0
Identity = 502/733 (68.49%), Postives = 595/733 (81.17%), Query Frame = 0

Query:  121 AFLYRHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQ--KDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNK-VEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYASGERKFLHQQLPLLDVD 850
            AFL R+Q+ N+K +VIK+GSAVITR DGNGLALGRLASIVEQV+ELQN G  CILITSGAVAFGKQKL+QE+MMSMSMRET+  N  +KEDI  +M  S KKPNAAVGQSGLQALYE MFRNY I+VGQVLVTK DFY+ ++R QLF TI EL+ LNIIPIINTNDAVSPP     ++D    + IKDNDSLAARVAVE  ADLAILMSDVDGIYD  P+ E A+++HT++ +  ++ +EFG KSD GTGGMESKV+SA WA  NG SVVICNGMK+NTI+K+                          AR G R+L +L+P ERA II+ IANSL+ RQ+EIM+VN+ DL+ A  + +   + SRL F+  KI SLA GLKQIA+TS+ NVG+V+RRTK+SD+++L+QKTVPIGVLMVIFESRPDA PQVASL+IA++NGLL+KGGKEA ++N LLMNIVKEAL  YGCADAISMVS+REA+GDLLKM +YIDLVIPRGS ELV  IKEQSKMIPVLGHAEG+CHVYVD+DAD+EKAV IV DSKTDYPAACNAMETLL HEDLL    FDKIC  LK++GV I +GP LA+ LTFGPP A  L++EYGD+ACTVE+VK +Y+AIDHIHK+GS HTD IV+EN +T + F  SVDSACVF+NCS+RM+DGYR+GLGAEVGISTGRIHARGPVGVEGLLTTKW+L+G GDTA  YA G+R F+HQ +P+ +V+
Sbjct:  120 AFLQRNQIPNSKSVVIKLGSAVITRSDGNGLALGRLASIVEQVAELQNRGIHCILITSGAVAFGKQKLSQELMMSMSMRETLHNNRMSKEDINDMMKSSLKKPNAAVGQSGLQALYEMMFRNYSIIVGQVLVTKADFYDVNSRTQLFNTIRELICLNIIPIINTNDAVSPPMAMATEDDSSEFIAIKDNDSLAARVAVELNADLAILMSDVDGIYDXPPTEEGAQILHTYSVSSRSQDIEFGEKSDSGTGGMESKVKSASWACNNGVSVVICNGMKHNTIQKVFA------------------------ARNGXRKLLSLSPIERADIISKIANSLVDRQEEIMAVNQKDLKMALKNELSPPLLSRLKFTKAKIISLADGLKQIADTSYENVGKVIRRTKVSDSLDLLQKTVPIGVLMVIFESRPDAXPQVASLSIATSNGLLLKGGKEAAHTNSLLMNIVKEALGTYGCADAISMVSSREAVGDLLKMSEYIDLVIPRGSNELVSMIKEQSKMIPVLGHAEGVCHVYVDRDADIEKAVKIVVDSKTDYPAACNAMETLLFHEDLLDYG-FDKICHALKDAGVLIHAGPNLAKKLTFGPPLAPRLRYEYGDLACTVELVKNVYEAIDHIHKYGSGHTDAIVSENSDTLECFKNSVDSACVFTNCSTRMSDGYRMGLGAEVGISTGRIHARGPVGVEGLLTTKWLLSGNGDTAQEYAKGDRAFIHQHIPVENVN 827          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. L. salmonis genes
Match: EMLSAG00000003759 (supercontig:LSalAtl2s:LSalAtl2s202:143959:144647:-1 gene:EMLSAG00000003759 transcript:EMLSAT00000003759 description:"snap_masked-LSalAtl2s202-processed-gene-1.2")

HSP 1 Score: 72.7886 bits (177), Expect = 5.056e-16
Identity = 37/65 (56.92%), Postives = 45/65 (69.23%), Query Frame = 0

Query:  636 GHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGV 700
            GH E +CHVYVD+DAD+EKAV IV D KTDY AA N +  L +HEDLL    FDK+C     +G+
Sbjct:   10 GHTEWVCHVYVDRDADIEKAVEIVVDFKTDYSAAWNCI-PLPLHEDLLDEG-FDKLCHVFINAGL 72          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. SwissProt
Match: gi|75054705|sp|Q5R4M8.1|P5CS_PONAB (RecName: Full=Delta-1-pyrroline-5-carboxylate synthase; Short=P5CS; AltName: Full=Aldehyde dehydrogenase family 18 member A1; Includes: RecName: Full=Glutamate 5-kinase; Short=GK; AltName: Full=Gamma-glutamyl kinase; Includes: RecName: Full=Gamma-glutamyl phosphate reductase; Short=GPR; AltName: Full=Glutamate-5-semialdehyde dehydrogenase; AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase)

HSP 1 Score: 798.505 bits (2061), Expect = 0.000e+0
Identity = 395/760 (51.97%), Postives = 550/760 (72.37%), Query Frame = 0

Query:  100 VKKYGSLPMFSGSDTHSETKSAFLYRHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGV--LGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNG----MKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYAS-GERKFLHQQLPLLDVDAN 852
            V+ + ++P  +   + +  KS F +R +L +AKRIV+K+GSAV+TR D  GLALGRLASIVEQVS LQN+GRE +L+TSGAVAFGKQ+L  E+++S S+R+ +    N  +++   + ++  +  AA GQSGL ALYE MF  Y I   Q+LVT  DF+++  R  L  T++ELL++NI+PI+NTNDAV PP + + D +GV  + +KDNDSLAAR+AVE + DL I++SDV+G++D  P  +DA+++  F P D   V FG KS +G GGME+KV++ALWAL+ G+SVVI NG    +  + I  I+ G+KVG+FF++ +     VE   + AR+G R L  L P +RA+II+ +A+ L  ++DEI+  N+ DL +A    +   +  RL+ S  K+ SLA GL+QIA +S ++VGRV+RRT+I+  + L Q TVPIGVL+VIFESRPD LPQVA+LAIAS NGLL+KGGKEA +SN +L  + +EAL+ +G  +A+ +V+ RE + DL ++D+ IDL+IPRGS +LV  I++ +K IPV+GH+EGICH+YVD +A ++K   +V+DSK +YPAACNA+ETLLIH DLL+TP+FD+I   L+   V I +GP  A +LTF P +  +L+ EYGD+   +E+V  + DAIDHIHK+GSSHTDVIVTENE T++ FL+ VDSACVF N S+R +DGYR GLGAEVGIST RIHARGPVG+EGLLTTKW+L G+    + ++  G  K+LH+ LP+   + N
Sbjct:   40 VRSWSNIPFITVPLSRTHGKS-FAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEA--RACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGDQQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIIHHLADLLTDQRDEILLANKKDLEEAE-GRLAPPLLKRLSLSTSKLNSLAIGLRQIAASSQDSVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVKEAVQLVNTREEVEDLCRLDKMIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKSLRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIVTENENTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGKDHVVSDFSEHGSLKYLHENLPIPQRNTN 795          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. SwissProt
Match: gi|6226882|sp|P54886.2|P5CS_HUMAN (RecName: Full=Delta-1-pyrroline-5-carboxylate synthase; Short=P5CS; AltName: Full=Aldehyde dehydrogenase family 18 member A1; Includes: RecName: Full=Glutamate 5-kinase; Short=GK; AltName: Full=Gamma-glutamyl kinase; Includes: RecName: Full=Gamma-glutamyl phosphate reductase; Short=GPR; AltName: Full=Glutamate-5-semialdehyde dehydrogenase; AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase)

HSP 1 Score: 797.734 bits (2059), Expect = 0.000e+0
Identity = 396/761 (52.04%), Postives = 553/761 (72.67%), Query Frame = 0

Query:  100 VKKYGSLPMFSGSDTHSETKSAFLYRHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGV--LGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNG----MKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGA-MYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYAS-GERKFLHQQLPLLDVDAN 852
            V+ + ++P  +   + +  KS F +R +L +AKRIV+K+GSAV+TR D  GLALGRLASIVEQVS LQN+GRE +L+TSGAVAFGKQ+L  E+++S S+R+ +    N  +++   + ++  +  AA GQSGL ALYE MF  Y I   Q+LVT  DF+++  R  L  T++ELL++NI+PI+NTNDAV PP + + D +GV  + +KDNDSLAAR+AVE + DL I++SDV+G++D  P  +DA+++  F P D   V FG KS +G GGME+KV++ALWAL+ G+SVVI NG    +  + I  I+ G+KVG+FF++ +     VE   + AR+G R L  L P +RA+II+ +A+ L  ++DEI+  N+ DL +A  +G + A +  RL+ S  K+ SLA GL+QIA +S ++VGRV+RRT+I+  + L Q TVPIGVL+VIFESRPD LPQVA+LAIAS NGLL+KGGKEA +SN +L  + +EAL+ +G  +A+ +V+ RE + DL ++D+ IDL+IPRGS +LV  I++ +K IPV+GH+EGICH+YVD +A ++K   +V+DSK +YPAACNA+ETLLIH DLL+TP+FD+I   L+   V I +GP  A +LTF P +  +L+ EYGD+   +E+V  + DAIDHIHK+GSSHTDVIVTE+E T++ FL+ VDSACVF N S+R +DGYR GLGAEVGIST RIHARGPVG+EGLLTTKW+L G+    + ++  G  K+LH+ LP+   + N
Sbjct:   40 VRSWSNIPFITVPLSRTHGKS-FAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNQLKEMAIPVLEA--RACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGDQQSVTFGTKSRVGMGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIIHHLADLLTDQRDEILLANKKDLEEA--EGRLAAPLLKRLSLSTSKLNSLAIGLRQIAASSQDSVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVKEAVQLVNTREEVEDLCRLDKMIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKSLRTEYGDLELCIEVVDNVQDAIDHIHKYGSSHTDVIVTEDENTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGKDHVVSDFSEHGSLKYLHENLPIPQRNTN 795          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. SwissProt
Match: gi|341941220|sp|Q9Z110.2|P5CS_MOUSE (RecName: Full=Delta-1-pyrroline-5-carboxylate synthase; Short=P5CS; AltName: Full=Aldehyde dehydrogenase family 18 member A1; Includes: RecName: Full=Glutamate 5-kinase; Short=GK; AltName: Full=Gamma-glutamyl kinase; Includes: RecName: Full=Gamma-glutamyl phosphate reductase; Short=GPR; AltName: Full=Glutamate-5-semialdehyde dehydrogenase; AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase)

HSP 1 Score: 797.734 bits (2059), Expect = 0.000e+0
Identity = 394/754 (52.25%), Postives = 547/754 (72.55%), Query Frame = 0

Query:  100 VKKYGSLPMFSGSDTHSETKSAFLYRHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGV--LGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNG----MKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYAS-GERKFLHQQLPL 846
            V+ + ++P  +   + +  K  F +R +L +AKRIV+K+GSAV+TR D  GLALGRLASIVEQVS LQN+GRE +L+TSGAVAFGKQ+L  E+++S S+R+ +    N  +++   + ++  +  AA GQSGL ALYE MF  Y I   Q+LVT  DF+++  R  L  T++ELL++NI+PI+NTNDAV PP + + D +GV  + +KDNDSLAAR+AVE + DL I++SDV+G++D  P  +DA+++  F P D   V FG KS +G GGME+KV++ALWAL+ G+SVVI NG    +  + I  I+ G+KVG+FF++ +     VE   + AR+G R L  L P +RA+IIN +A+ L  +++EI+  N+ DL +A    +   +  RL+ S  K+ SLA GL+QIA +S  +VGRV+RRT+I+  + L Q TVPIGVL+VIFESRPD LPQVA+LAIAS NGLL+KGGKEA +SN +L  + +EAL+ +G  +AI +V+ RE + DL ++D+ IDL+IPRGS +LV  I++ +K IPV+GH+EGICH+YVD +A ++K   +V+DSK +YPAACNA+ETLLIH DLL+TP+FD+I   L+   V I +GP  A +LTF P +  +L+ EYGD+   +E+V  + +AIDHIHK+GSSHTDVIVTENE+T++ FL+ VDSACVF N S+R +DGYR GLGAEVGIST RIHARGPVG+EGLLTTKW+L GQ    + ++  G  K+LH+ LP+
Sbjct:   40 VRSWSNIPFITVPLSRAHGK-PFAHRSELKHAKRIVVKLGSAVVTRGDECGLALGRLASIVEQVSVLQNQGREMMLVTSGAVAFGKQRLRHEILLSQSVRQALHSGQNHLKEMAIPVLEA--RACAAAGQSGLMALYEAMFTQYSICAAQILVTNLDFHDEQKRRNLNGTLHELLRMNIVPIVNTNDAVVPPAEPNSDLQGVNVISVKDNDSLAARLAVEMKTDLLIVLSDVEGLFDSPPGSDDAKLIDIFYPGDQQSVTFGTKSRVGLGGMEAKVKAALWALQGGTSVVIANGTHPKVSGHVITDIVEGKKVGTFFSEVKPAGPTVEQQGEMARSGGRMLATLEPEQRAEIINHLADLLTDQREEILLANKKDLEEAE-GRLASPLLKRLSLSTSKLNSLAIGLRQIAASSQESVGRVLRRTRIAKNLELEQVTVPIGVLLVIFESRPDCLPQVAALAIASGNGLLLKGGKEAAHSNRILHLLTQEALSIHGVKEAIQLVNTREEVEDLCRLDKIIDLIIPRGSSQLVRDIQKAAKGIPVMGHSEGICHMYVDSEASVDKVTRLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAGPKFASYLTFSPSEVKSLRTEYGDLEVCIEVVDSVQEAIDHIHKYGSSHTDVIVTENEKTAEFFLQHVDSACVFWNASTRFSDGYRFGLGAEVGISTSRIHARGPVGLEGLLTTKWLLRGQDHVVSDFSEHGSLKYLHENLPV 789          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. SwissProt
Match: gi|544584725|sp|P54889.2|ALH13_CAEEL (RecName: Full=Probable delta-1-pyrroline-5-carboxylate synthase; Short=P5CS; Includes: RecName: Full=Glutamate 5-kinase; Short=GK; AltName: Full=Gamma-glutamyl kinase; Includes: RecName: Full=Gamma-glutamyl phosphate reductase; Short=GPR; AltName: Full=Aldehyde dehydrogenase family 13; AltName: Full=Glutamate-5-semialdehyde dehydrogenase; AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase)

HSP 1 Score: 747.273 bits (1928), Expect = 0.000e+0
Identity = 372/723 (51.45%), Postives = 505/723 (69.85%), Query Frame = 0

Query:  125 RHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAE-QDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYG--CADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYASGERKFLHQQL 844
            R+ L  A+R+V+K+GSAVITRED  GLALGRLASIVEQVSELQ  GR+ ++++SGAVAFG+QKL QE++MSMSMR+T+ G      D         K+  AA G  GL +LYE +F+ YGI V QVL+TKPD  +D  R+ L  TI  LL LNIIPI+N NDAV+P      DP+  + I DNDSLAAR++ E  A+L I++S+V+G+Y   P  E +R+++T+ P++ + V FG  S  GTGGMESKV + + AL NG + VI NG+  + I   + G+K+G+ F   +  +  P+E +A+  R   R+L AL+  ER  ++  +A  L+ ++  I+  N+ DL  A+  G+   + +RL  +P+KIQ L  GL  IA+++   VGRV+++ KIS+ + L Q TVPIG LMVIFESRPD LPQVASLA+AS N LL+KGGKEA  SN  L  +V+EAL  +G    DA+++V +RE + DLL++   IDL+IPRGS +LV +++E+SK IPVLGHAEG+CHVY+DKD D +KA+ IV+DSK DYP+ACNA ET+LIH+DL   P FD +C+  K  GV + +GP LA  L F PP A ++  EYG + CT+E+V  + +A+ HI ++GS HT+ I+TEN  T++ FL+ VDSAC F N S+R ADGYR GLGAEVGISTGRIHARGPVGVEGLLTTKW+L G+G     + +G+  +LH+ L
Sbjct:   75 RNDLKKAQRVVVKLGSAVITREDECGLALGRLASIVEQVSELQQSGRQMLIVSSGAVAFGRQKLRQELVMSMSMRQTLRGPSGMTAD---------KRACAASGMPGLMSLYEQLFQQYGITVAQVLLTKPDIDDDQRRKNLQATIESLLSLNIIPIVNANDAVAP------DPKLNMHISDNDSLAARLSAEIEAELLIILSNVNGVYTGPPDLEGSRLLYTYVPSENSGVTFGANSKFGTGGMESKVTACVNALNNGVTTVITNGLAQDAITDAVAGKKIGTMFCNTKGYEGPPIEEVAEKCRDAGRQLAALSNKERGAMVRHLAALLVDKEKYIIEANQTDLANAKSAGLDPQLLNRLKMTPEKIQDLHAGLNTIADSAETLVGRVLKKVKISEGLFLEQVTVPIGSLMVIFESRPDCLPQVASLAMASGNALLLKGGKEAEESNKALHALVQEALGTHGFEMRDAVTLVRSREDVADLLQLKDLIDLIIPRGSSDLVRSMQEKSKGIPVLGHAEGVCHVYIDKDCDEQKAIQIVRDSKCDYPSACNAAETILIHKDLATAPFFDSLCSMFKAEGVKLHAGPKLAALLKFAPPPAESMSFEYGSLECTLEVVDNVEEAVAHIIRYGSGHTESIITENTNTAEHFLKHVDSACAFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWLLRGEGHLVEDFKNGKYSYLHENL 782          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. SwissProt
Match: gi|115510969|sp|O04226.2|P5CS1_ORYSJ (RecName: Full=Delta-1-pyrroline-5-carboxylate synthase 1; Short=OsP5CS1; Includes: RecName: Full=Glutamate 5-kinase; Short=GK; AltName: Full=Gamma-glutamyl kinase; Includes: RecName: Full=Gamma-glutamyl phosphate reductase; Short=GPR; AltName: Full=Glutamate-5-semialdehyde dehydrogenase; AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase)

HSP 1 Score: 635.18 bits (1637), Expect = 0.000e+0
Identity = 340/731 (46.51%), Postives = 476/731 (65.12%), Query Frame = 0

Query:  125 RHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNK-VEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTK------AEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYASGERK--FLHQQLPL 846
            R  + + KR++IK+G+AV++R+DG  LALGR+ ++ EQV EL + G E IL+TSGAV  G+Q+L    +++ S             D++    +   K  AAVGQSGL ALY+ +F    +   Q+LVT  DF N   REQL  T+  LL L +IPI N NDA+S      ED  G+    DNDSLA  +A+E +ADL IL+SDVDG+Y   PS   ++++HT+      + + FG KS +G GGM +KV++A+ A  +G+ VVI +G +  +I K++ G+K+G+ F K      + +D    E +A  AR  SR LQ L+  ER KI+  +A++L + +D I S NE D+  A++ G    + +RL   P KI SLA  ++ +A    + + +++++T+++D + L + + P+GVL+++FESRPDAL Q+ASLAI S NGLL+KGGKEA  SN +L  ++ +A+        I +V+ R+ I DLLK+D  IDLVIPRGS +LV  IK  +K IPVLGHA+GICHVY+DK AD++ A  IV D+K DYPAACNAMETLL+H+DL+K+P  D I   LK  GV I+ GP    H   G P+A +  HEY  MACTVE V  +  AIDHIH++GS+HTD IVT +++ ++ FL  VDSA VF N S+R +DG R GLGAEVGISTGRIHARGPVGVEGLLTT+W+L G+G       +G++   + H+ LPL
Sbjct:    8 RSFVRDVKRVIIKVGTAVVSRQDGR-LALGRVGALCEQVKELNSLGYEVILVTSGAVGVGRQRLRYRKLVNSSF-----------ADLQKPQMELDGKACAAVGQSGLMALYDMLFNQLDVSSSQLLVTDSDFENPKFREQLTETVESLLDLKVIPIFNENDAISTRKAPYEDSSGIFW--DNDSLAGLLALELKADLLILLSDVDGLYSGPPSEPSSKIIHTYIKEKHQQEITFGDKSRVGRGGMTAKVKAAVLASNSGTPVVITSGFENRSILKVLHGEKIGTLFHKNANLWESSKDVSTRE-MAVAARDCSRHLQNLSSEERKKILLDVADALEANEDLIRSENEADVAAAQVAGYEKPLVARLTIKPGKIASLAKSIRTLANME-DPINQILKKTEVADDLVLEKTSCPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEAIRSNTILHKVITDAIPRNVGEKLIGLVTTRDEIADLLKLDDVIDLVIPRGSNKLVSQIKASTK-IPVLGHADGICHVYIDKSADMDMAKHIVMDAKIDYPAACNAMETLLVHKDLMKSPGLDDILVALKTEGVNIYGGPI--AHKALGFPKAVSFHHEYSSMACTVEFVDDVQSAIDHIHRYGSAHTDCIVTTDDKVAETFLRRVDSAAVFHNASTRFSDGARFGLGAEVGISTGRIHARGPVGVEGLLTTRWILRGRGQV----VNGDKDVVYTHKSLPL 715          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. SwissProt
Match: gi|6225817|sp|Q96480.1|P5CS_SOLLC (RecName: Full=Delta-1-pyrroline-5-carboxylate synthase; Short=P5CS; Includes: RecName: Full=Glutamate 5-kinase; Short=GK; AltName: Full=Gamma-glutamyl kinase; Includes: RecName: Full=Gamma-glutamyl phosphate reductase; Short=GPR; AltName: Full=Glutamate-5-semialdehyde dehydrogenase; AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase)

HSP 1 Score: 603.979 bits (1556), Expect = 0.000e+0
Identity = 331/706 (46.88%), Postives = 466/706 (66.01%), Query Frame = 0

Query:  130 NAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKV-EFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFF--------TKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQG 826
            N KR+++K+G+AV+TR DG  LALGRL ++ EQ+ EL ++G E IL+TSGAV  G+Q+L    +++ S             D++    +   K  AAVGQ+GL ALY+++F    +   Q+LVT  DF + D R QL  T+N LL L +IPI N NDA+S      ED  G+    DNDSLAA +A+E +ADL +L+SDVDG+Y   P   D+++++T+      +V  FG KS +G GGM +KV++A++A   G  VVI +G   + I K++ G+++G+ F        +  E DA     +A  ARA SRRLQAL+  ER+KI+  IA++L + +  I++ NE D+  A+  G   ++ SRLA +P KI SLA  ++ ++      +G  ++RT+I+D   L + + P+GV+++IFESRPDAL Q+ASLA+ S NGL++KGGKEA  SN +L  ++  A+        I +V++RE I +LLK+D  IDLVIPRGS +LV  IK  +K IPVLGHA+GICHVYVDK AD++ A  I  D+K DYPAACNAMETLL+H+DL +    + +   L+  GV+++ GP  +  L    P+A   +HEY  +ACTVE+V+ +Y AIDHIH+ GS+HTD I+TE++E ++ FL  VDSA VF N S+R +DG+R GLGAEVGISTGRIHARGPVGVEGLLTTKW+  G G
Sbjct:   13 NVKRLIVKVGTAVVTRADGR-LALGRLGALCEQLQELNSQGYEVILVTSGAVGVGRQRLRYRKLLNSSFL-----------DLQKPQTELDGKACAAVGQNGLMALYDSLFSQLDVTSAQLLVTDNDFRDPDFRRQLNDTVNSLLSLKVIPIFNENDAISTRRAPYEDSSGIFW--DNDSLAALLALELKADLLVLLSDVDGLYSGPPRDPDSKLIYTYIKEIHERVITFGDKSRVGRGGMTAKVKAAMYAAYAGIPVVITSGFATDNIIKVLHGERIGTLFHCDANKWASIGETDARE---MAVAARACSRRLQALSSQERSKILQDIADALEANEKAILAENEADVVAAQQAGYEKSLISRLALNPGKISSLANSVRVLSNMD-EPLGHTLKRTEIADGFILEKSSSPLGVVLIIFESRPDALVQIASLAVRSGNGLMLKGGKEAKRSNAILHKVITSAIPVSVGERLIGLVTSREEIPELLKLDDVIDLVIPRGSNKLVSQIKASTK-IPVLGHADGICHVYVDKSADMDMAKRITVDAKIDYPAACNAMETLLVHKDLAQNGGLNDLIVELQTKGVSLYGGPKASSLLMI--PEARTFRHEYSSLACTVEVVEDVYAAIDHIHQHGSAHTDSIITEDQEVAEVFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWLARGSG 697          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. SwissProt
Match: gi|1709535|sp|P54888.1|P5CS2_ARATH (RecName: Full=Delta-1-pyrroline-5-carboxylate synthase B; Short=P5CS B; Includes: RecName: Full=Glutamate 5-kinase; Short=GK; AltName: Full=Gamma-glutamyl kinase; Includes: RecName: Full=Gamma-glutamyl phosphate reductase; Short=GPR; AltName: Full=Glutamate-5-semialdehyde dehydrogenase; AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase)

HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0
Identity = 335/726 (46.14%), Postives = 473/726 (65.15%), Query Frame = 0

Query:  130 NAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTF-NPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPV-----EVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYASGERK--FLHQQLPLL 847
            + KRIV+K+G+AV+T + G  LALGRL +I EQ++EL ++G E IL++SGAV  G+Q+L          R+ ++ ++    D++    +   K  A VGQS L A YETMF    + V Q+LVT   F + D R+QL  T+  +L++ +IP+ N NDA+S      +D  G+    DNDSLAA +++E +ADL IL+SDV+G+Y   PS   ++++HTF      +++ FG KS LG GGM +KV++A+ A   G  V+I +G     I K++RG +VG+ F +      PV       +A  AR  SR+LQAL+  +R +I++ IAN+L   +  I + N+LD+  A+  G   ++ +RL   P KI SLA  ++Q+AE   + +GRV+++T+++D + L + + PIGVL+++FESRPDAL Q+ASLAI S NGLL+KGGKEA  SN +L  ++ +A+ E      I +V++RE I DLLK+D  IDLVIPRGS +LV  IK  +K IPVLGHA+GICHVYVDK   L+ A  IV D+K DYPAACNAMETLL+H+DL +    D +   L+  GV ++ GP  +  L    P+  +  HEY   ACTVEIV+ +Y AIDHIH+ GS+HTD IVTE+ E ++ FL  VDSA VF N S+R +DG+R GLGAEVGIST RIHARGPVGVEGLLTT+W++ G+G        G+    + H+ LP+L
Sbjct:   13 DVKRIVVKVGTAVVTGKGGR-LALGRLGAICEQLAELNSDGFEVILVSSGAVGLGRQRL--------RYRQLVNSSF---ADLQKPQMELDGKACAGVGQSSLMAYYETMFDQLDVTVAQMLVTDSSFRDKDFRKQLSETVKAMLRMRVIPVFNENDAISTRRAPYKDSTGIFW--DNDSLAALLSLELKADLLILLSDVEGLYTGPPSDSTSKLIHTFIKEKHQDEITFGEKSKLGRGGMTAKVKAAVNAAYGGVPVIITSGYAAENISKVLRGLRVGTLFHQDAHLWAPVVDTTSRDMAVAARESSRKLQALSSEDRKQILHDIANALEVNEKTIKAENDLDVAAAQEAGYEESLVARLVMKPGKISSLAASVRQLAEME-DPIGRVLKKTQVADDLILEKTSSPIGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKNSTK-IPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNGFLDDLIYVLQTKGVTLYGGPRASAKLNI--PETKSFHHEYSSKACTVEIVEDVYGAIDHIHQHGSAHTDCIVTEDSEVAEIFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGISTSRIHARGPVGVEGLLTTRWIMRGKGQV----VDGDNGIVYTHKDLPVL 716          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. SwissProt
Match: gi|75249542|sp|Q941T1.1|P5CS2_ORYSJ (RecName: Full=Delta-1-pyrroline-5-carboxylate synthase 2; Short=OsP5CS2; Includes: RecName: Full=Glutamate 5-kinase; Short=GK; AltName: Full=Gamma-glutamyl kinase; Includes: RecName: Full=Gamma-glutamyl phosphate reductase; Short=GPR; AltName: Full=Glutamate-5-semialdehyde dehydrogenase; AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase)

HSP 1 Score: 597.43 bits (1539), Expect = 0.000e+0
Identity = 330/725 (45.52%), Postives = 463/725 (63.86%), Query Frame = 0

Query:  130 NAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNK-VEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTK-AEQDAMPVEVLAKN----ARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYASGERK--FLHQQLPL 846
            + KRI+IK+G+AV+T  +G  LA+GRL ++ EQV +L  EG E IL+TSGAV  G+Q+L    +++ S  +      N + D+         K  AAVGQS L A+Y+T+F    +   Q+LVT  DF +     QL  T+N LL L +IP+ N NDA+S   Q  ED  G+    DNDSLA  +A E +ADL I++SDV+G+Y   PS   ++++HT+      K + FG KS +G GGM++KV +A  A   G  VVI +G   ++I K+MRG+K+G+ F + A Q     E  A+     AR  SR LQ L+  ER KI+  IA++L + +D I S N+ DL  A+  G   ++ +R+   P KI+SLA  +++IA+   + +   ++RT+++  +   +   P+GVL++IFESRPDAL Q+ASLAI S NGLL+KGGKEA  SN +L  ++  A+ +      I +V N++ I DLLK+D  IDLVIPRGS +LV  IK  +K IPVLGHA+GICHVY+DK AD++ A  IV D+K DYPAACNAMETLL+H+DL +T   D +   L++ GV I+ GP    H T   P+  +  HEY  MACT+E V  +  AIDHI+++GS+HTD I+T + + ++ FL+ VDSA VF N S+R  DG R GLGAEVGISTGRIHARGPVGV+GLLTT+ +L G G       +G++   + H++LPL
Sbjct:   32 DVKRIIIKVGTAVVTGPNGR-LAMGRLGALCEQVKQLNFEGYEVILVTSGAVGVGRQRLKYRKLVNSSFADL----QNPQMDMDG-------KACAAVGQSVLMAIYDTLFSQLDVTSSQLLVTDRDFMDPSFGNQLRETVNSLLDLKVIPVFNENDAISTRRQPYEDSSGIFW--DNDSLARLLAQELKADLLIMLSDVEGLYSGPPSDPQSKIIHTYVHEQHGKLISFGEKSRVGRGGMQAKVAAAFTASSKGIPVVIASGFAIDSIIKVMRGEKIGTLFHREANQWGCSKEATAREMAVAARDCSRHLQKLSSEERKKILLDIADALEANEDLITSENQADLDLAQDIGYDKSLVARMTIKPGKIKSLAGSIREIADME-DPISHTLKRTEVAKDLVFEKTYCPLGVLLIIFESRPDALVQIASLAIRSGNGLLLKGGKEAMRSNTILHKVITGAIPDVVGKKLIGLVKNKDEIADLLKLDDVIDLVIPRGSNKLVSQIKAATK-IPVLGHADGICHVYIDKSADMDMAKRIVLDAKVDYPAACNAMETLLVHKDLNRTEGLDDLLVELEKEGVVIYGGPV--AHDTLKLPKVDSFHHEYNSMACTLEFVDDVQSAIDHINRYGSAHTDCIITTDGKAAETFLQQVDSAAVFHNASTRFCDGARFGLGAEVGISTGRIHARGPVGVDGLLTTRCILRGSGQV----VNGDKGVVYTHRELPL 734          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. SwissProt
Match: gi|6225816|sp|O04015.1|P5CS_ACTDE (RecName: Full=Delta-1-pyrroline-5-carboxylate synthase; Short=P5CS; Includes: RecName: Full=Glutamate 5-kinase; Short=GK; AltName: Full=Gamma-glutamyl kinase; Includes: RecName: Full=Gamma-glutamyl phosphate reductase; Short=GPR; AltName: Full=Glutamate-5-semialdehyde dehydrogenase; AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase)

HSP 1 Score: 595.119 bits (1533), Expect = 0.000e+0
Identity = 336/701 (47.93%), Postives = 467/701 (66.62%), Query Frame = 0

Query:  132 KRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNK-VEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPV-EVLAKN----ARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQG 826
            KR++IK+G+AV+TR DG  LALGRL ++ EQ+ EL ++G E IL+TSGAV  G+Q+L    +++ S             D++    +   K  AAVGQ+GL ALY+T+F    +   Q+LVT  DF + + R+QL  T+  LL L +IPI N NDAVS      ED  G+    DNDSLAA +A+E +ADL +L+SDV+G+Y   PS   ++++HT+    F   + FG KS +G GGM +KV++A++A   G  VVI +G   N I K+++G+++G+ F +  Q   PV +V A++    AR  SRRLQA++P +R+KI+  +A++L + +  I   NE DL  A+  G   ++ SRLA    KI SLA  ++ +A      +G V++RT+I+D + L + + P+GVL++IFESRPDAL Q+ASLAI S NGL++KGGKEA  SN +L  ++  A+ E      I +V++RE I DLLK+D  IDLVIPRGS +LV  IKE +K IPVLGHA+GICHVYVDK A+++ A  +V D+KTDYPAACNAMETLL+H+DL++    D++   L+  GV I  GP  +  L    P+A +L HEY  +ACT+EIV  +Y AIDHIH+ GS+HTD I+TE+ E ++ FL  VDS+ V  N S+R +DG R GLGAEVGIST RIHARGPVGVEGLLTT+W+  G G
Sbjct:   15 KRVIIKVGTAVVTRADGR-LALGRLGALCEQIHELNSQGFEVILVTSGAVGVGRQRLRYRKLVNSSF-----------ADLQKPQIELDGKACAAVGQNGLLALYDTLFSQLDVTSAQLLVTDNDFRDPEFRKQLTETVESLLNLKVIPIFNENDAVSTRKAPYEDASGIFW--DNDSLAALLALELKADLLVLLSDVEGLYSGPPSDPQSKLIHTYIKEMFEGLITFGDKSRVGRGGMTAKVKAAVYAAHAGIPVVITSGYATNNIIKVLQGERIGTLFHRDAQKWAPVGDVGARDMAVAARESSRRLQAMSPQDRSKILLDVADALEANEKLIRIENEADLAAAQQAGYEKSLISRLALKSGKISSLAKSIRVLANME-EPIGHVLKRTEITDGLVLEKTSSPLGVLLIIFESRPDALVQIASLAIRSGNGLVLKGGKEAKRSNAILHKVITSAIPENVGPRLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTK-IPVLGHADGICHVYVDKSANMDMAKKVVLDAKTDYPAACNAMETLLVHKDLVQNGCLDELIVELQIKGVVIHGGPRASSLLHI--PEARSLHHEYSSLACTIEIVDDVYAAIDHIHRHGSAHTDSIITEDHEVAEIFLRQVDSSSVLHNASTRFSDGARFGLGAEVGISTSRIHARGPVGVEGLLTTRWIARGSG 697          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. SwissProt
Match: gi|1709534|sp|P54887.1|P5CS1_ARATH (RecName: Full=Delta-1-pyrroline-5-carboxylate synthase A; Short=P5CS A; Includes: RecName: Full=Glutamate 5-kinase; Short=GK; AltName: Full=Gamma-glutamyl kinase; Includes: RecName: Full=Gamma-glutamyl phosphate reductase; Short=GPR; AltName: Full=Glutamate-5-semialdehyde dehydrogenase; AltName: Full=Glutamyl-gamma-semialdehyde dehydrogenase)

HSP 1 Score: 590.497 bits (1521), Expect = 0.000e+0
Identity = 331/725 (45.66%), Postives = 478/725 (65.93%), Query Frame = 0

Query:  130 NAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTF-NPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPV-----EVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYASGERK--FLHQQLPL 846
            + KRIV+K+G+AV+T + G  LALGRL ++ EQ++EL ++G E IL++SGAV  G+Q+L          R+ ++ ++    D++    +   K  A VGQS L A YETMF    +   Q+LV    F + D R+QL  T+  +L L +IPI N NDA+S      +D  G+    DNDSLAA +A+E +ADL IL+SDV+G+Y   PS  +++++HTF      +++ FG KS LG GGM +KV++A+ A   G  V+I +G     I K++RG +VG+ F +  +   P+       +A  AR  SR+LQAL+  +R KI+  IA++L +    I + NELD+  A+  G+  +M +RL  +P KI SLA  ++++A+   + +GRV+++T+++D + L + + P+GVL+++FESRPDAL Q+ASLAI S NGLL+KGGKEA  SN +L  ++ +A+ E      I +V++RE I DLLK+D  IDLVIPRGS +LV  IK  +K IPVLGHA+GICHVYVDK  D + A  IV D+K DYPAACNAMETLL+H+DL +  + +++   L+ +GV ++ GP  ++ L    P+A +  HEY   ACTVE+V+ +Y AIDHIH+ GS+HTD IVTE+ E ++ FL  VDSA VF N S+R +DG+R GLGAEVG+STGRIHARGPVGVEGLLTT+W++ G+G        G+    + HQ +P+
Sbjct:   13 DVKRIVVKVGTAVVTGKGGR-LALGRLGALCEQLAELNSDGFEVILVSSGAVGLGRQRL--------RYRQLVNSSF---ADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLVNDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFW--DNDSLAALLALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKVKAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTPGKISSLAASVRKLADME-DPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTK-IPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNI--PEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQV----VDGDNGIVYTHQDIPI 715          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. nr
Match: gi|395318503|gb|AFN54351.1| (pyrroline-5-carboxylase synthase, partial [Tigriopus californicus])

HSP 1 Score: 1226.46 bits (3172), Expect = 0.000e+0
Identity = 587/642 (91.43%), Postives = 611/642 (95.17%), Query Frame = 0

Query:  185 KQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQG 826
            KQKLAQEVMMSMSMRETISGNYNTKEDIKTLM  SFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYND TR+QLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMH FNP D  KVEFG KSD GTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRG KVGSFFTKAE DAMPVEVLAKNAR+GSRRLQALAP  RAKIIN IA+SL+SRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQ+LATGLKQIA TS+ NVG+VVRRTK+SDTM+L+Q+TVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSN LLMNIVKEAL+EYGCADAISMVS REAIGDLLKMDQYIDLVIPRGSGELV + K Q  MIPVLGHAEG+CHVYVDK ADLEKA AIVKDSKTDYPAACNAMETLL+HEDLLKTP+FDKIC+TLKESGVAIFSGP LA+HLTFGPPQAH+LKHEYGDMACT+EIVK +YDAIDHIHKFGSSHTDVIVT+NEE +Q FLESVDSACVF+NCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVL G G
Sbjct:    1 KQKLAQEVMMSMSMRETISGNYNTKEDIKTLMKSSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDHTRQQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHNFNPVDLAKVEFGEKSDAGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGDKVGSFFTKAEVDAMPVEVLAKNARSGSRRLQALAPEARAKIINKIADSLISRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQALATGLKQIAATSYQNVGKVVRRTKVSDTMDLVQRTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNNLLMNIVKEALSEYGCADAISMVSKREAIGDLLKMDQYIDLVIPRGSGELVKSXKXQXXMIPVLGHAEGVCHVYVDKYADLEKARAIVKDSKTDYPAACNAMETLLVHEDLLKTPVFDKICSTLKESGVAIFSGPTLAKHLTFGPPQAHSLKHEYGDMACTIEIVKDMYDAIDHIHKFGSSHTDVIVTDNEENAQIFLESVDSACVFANCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLNGDG 642          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. nr
Match: gi|395318475|gb|AFN54337.1| (pyrroline-5-carboxylase synthase, partial [Tigriopus californicus] >gi|395318477|gb|AFN54338.1| pyrroline-5-carboxylase synthase, partial [Tigriopus californicus])

HSP 1 Score: 1218.37 bits (3151), Expect = 0.000e+0
Identity = 582/633 (91.94%), Postives = 607/633 (95.89%), Query Frame = 0

Query:  194 MSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQG 826
            MSMSMRETISGNYNTKEDIKTLM  SFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYND+TR+QLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMH FNP D  KVEFG KSD GTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRG KVGSFFTKAE DAMPVEVLAKNAR+GSRRLQALAP  RAKIIN IA+SL+SRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQ+LATGLKQIA TS+ NVG+VVRRTK+SDTM+L+Q+TVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSN LLMNIVKEAL+EYGCADAISMVS REAIGDLLKMDQYIDLVIPRGSGELV +IKEQSKMIPVLGHAEG+CHVYVDK ADLEKA AIVKDSKTDYPAACNAMETLL+HEDLLKTP+FDKIC+TLKESGVAIFSGP LA+HLTFGPPQAH+LKHEYGDMACT+EIVK +YDAIDHIHKFGSSHTDVIVT+NEE +Q FLESVDSACVF+NCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVL G G
Sbjct:    1 MSMSMRETISGNYNTKEDIKTLMKSSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDNTRQQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHNFNPVDLAKVEFGEKSDAGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGDKVGSFFTKAEVDAMPVEVLAKNARSGSRRLQALAPEARAKIINKIADSLISRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQALATGLKQIAATSYQNVGKVVRRTKVSDTMDLVQRTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNNLLMNIVKEALSEYGCADAISMVSKREAIGDLLKMDQYIDLVIPRGSGELVKSIKEQSKMIPVLGHAEGVCHVYVDKYADLEKARAIVKDSKTDYPAACNAMETLLVHEDLLKTPVFDKICSTLKESGVAIFSGPTLAKHLTFGPPQAHSLKHEYGDMACTIEIVKDMYDAIDHIHKFGSSHTDVIVTDNEENAQIFLESVDSACVFANCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLNGDG 633          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. nr
Match: gi|395318507|gb|AFN54353.1| (pyrroline-5-carboxylase synthase, partial [Tigriopus californicus] >gi|395318509|gb|AFN54354.1| pyrroline-5-carboxylase synthase, partial [Tigriopus californicus])

HSP 1 Score: 1159.05 bits (2997), Expect = 0.000e+0
Identity = 556/614 (90.55%), Postives = 580/614 (94.46%), Query Frame = 0

Query:  220 FKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYA 833
            FKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYND TR+QLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMH FNP D  KVEFG KSD GTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRG KVGSFFTKAE DAMPVEVLAKNAR+GSRRLQALAP  RAKIIN IA+SL+SRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQ+LATGLKQIA TS+ NVG+VVRRTK+SDTM+L+Q+TVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSN LLMNIVKEAL+EYGCADAISMVS  EAIG      QYIDLVIPRGSGELV +IKEQSKMIPVLGHAEG+CHVYVDK ADLEKA AIVKDSKTDYPAACNAMETLL+HEDLLKTP+FDKIC+TLKESGVAIFSGP LA+HLTFGPPQAH+LKHEYGDMACT+EIVK +YDAIDHIHKFGSSHTDVIVT+NEE +Q FLESVDSACVF+NCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVL G GD AA YA
Sbjct:    1 FKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDHTRQQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHNFNPVDLAKVEFGEKSDAGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGDKVGSFFTKAEVDAMPVEVLAKNARSGSRRLQALAPEARAKIINKIADSLISRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQALATGLKQIAATSYQNVGKVVRRTKVSDTMDLVQRTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNNLLMNIVKEALSEYGCADAISMVSXXEAIGXXXXXXQYIDLVIPRGSGELVKSIKEQSKMIPVLGHAEGVCHVYVDKYADLEKARAIVKDSKTDYPAACNAMETLLVHEDLLKTPVFDKICSTLKESGVAIFSGPTLAKHLTFGPPQAHSLKHEYGDMACTIEIVKDMYDAIDHIHKFGSSHTDVIVTDNEENAQIFLESVDSACVFANCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLNGDGDIAADYA 614          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. nr
Match: gi|395318483|gb|AFN54341.1| (pyrroline-5-carboxylase synthase, partial [Tigriopus californicus])

HSP 1 Score: 1153.66 bits (2983), Expect = 0.000e+0
Identity = 558/649 (85.98%), Postives = 583/649 (89.83%), Query Frame = 0

Query:  185 KQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYA 833
            KQKLAQEVMMSMSMRE ISGNYNTKEDIKTLM  SFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYND+TR+QLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMH FNP D  KVEFG KSD GTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRG KVGSFF KAE DA+PVEVLAKNAR+GSRRLQALAP  RAKIIN IA+SL+SRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQ+LATGLKQIA TS+ NVG+VVRRTK+SDTM+L+Q+TVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSN LLMNIVKEAL+EYGCADAISM                                      IPVLGHAEG+CHVYVDK ADLEKA AIVKDSKTDYPAACNAMETLL+HEDLLKTP+FDKIC+TLKESGVAIFSGP LA+HLTFGPPQAH+LKHEYGDMACT+EIVK +YDAIDHIHKFGSSHTDVIVT+NEE +Q FLESVDSACVF+NCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVL G GD AA YA
Sbjct:    1 KQKLAQEVMMSMSMREXISGNYNTKEDIKTLMKSSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDNTRQQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHNFNPVDLAKVEFGEKSDAGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGDKVGSFFXKAEVDALPVEVLAKNARSGSRRLQALAPEARAKIINKIADSLISRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQALATGLKQIAATSYQNVGKVVRRTKVSDTMDLVQRTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNNLLMNIVKEALSEYGCADAISMXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIPVLGHAEGVCHVYVDKYADLEKARAIVKDSKTDYPAACNAMETLLVHEDLLKTPVFDKICSTLKESGVAIFSGPTLAKHLTFGPPQAHSLKHEYGDMACTIEIVKDMYDAIDHIHKFGSSHTDVIVTDNEENAQIFLESVDSACVFANCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLNGDGDIAADYA 649          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. nr
Match: gi|395318493|gb|AFN54346.1| (pyrroline-5-carboxylase synthase, partial [Tigriopus californicus])

HSP 1 Score: 1131.32 bits (2925), Expect = 0.000e+0
Identity = 544/620 (87.74%), Postives = 567/620 (91.45%), Query Frame = 0

Query:  213 KTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAY 832
            KTLM  SFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYND TR+QLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMH FNP D  KVEFG KSD GTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRG K GSFFT                R+GSRRLQALAP  RAKIIN IA+SL+SRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQ+LATGLKQIA TS+ NVG+VVRRTK+SDTM+L+Q+TVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSN LLMNIVKEAL+EYGCADAISMVS REAIGDLLKMDQYIDLVIPRGSGELV         IPVLGHAEG+CHVYVDK ADLEKA AIVKDSKTDYPAACNAMETLL+HEDLLKTP+FDKIC+TLKESGVAIFSGP LA+HLTFGPPQAH+LKHEYGDMACT+EIVK +YDAIDHIHKFGSSHTDVIVT+NEE +Q FLESVDSACVF+NCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVL G GD AA Y
Sbjct:    1 KTLMKSSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDHTRQQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHNFNPVDLAKVEFGEKSDAGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGDKXGSFFTXXXXXXXXXXXXXXXXRSGSRRLQALAPEARAKIINKIADSLISRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQALATGLKQIAATSYQNVGKVVRRTKVSDTMDLVQRTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNNLLMNIVKEALSEYGCADAISMVSKREAIGDLLKMDQYIDLVIPRGSGELVKXXXXXXXXIPVLGHAEGVCHVYVDKYADLEKARAIVKDSKTDYPAACNAMETLLVHEDLLKTPVFDKICSTLKESGVAIFSGPTLAKHLTFGPPQAHSLKHEYGDMACTIEIVKDMYDAIDHIHKFGSSHTDVIVTDNEENAQIFLESVDSACVFANCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLNGDGDIAADY 620          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. nr
Match: gi|1325314459|ref|XP_023338906.1| (delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Eurytemora affinis] >gi|1325314461|ref|XP_023338907.1| delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 1025.39 bits (2650), Expect = 0.000e+0
Identity = 499/803 (62.14%), Postives = 614/803 (76.46%), Query Frame = 0

Query:   63 AAKLSSATKRSNDLLRSFPPKRLFFSRGLTTDGGPSGVKKYGS-------------------LPMFSGSDTHSETKSAFLYRHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYASGERKFLHQQLPL 846
            A +L +     + LL S PPK +   R  TT  G    ++Y +                   L   + S  H    S FL R +L NAKR+VIK+GSAV+TR DG GLALGRLA+I+EQVSE+QN G ECIL+TSGAVAFGKQ+L+QE++MSMSMRET+S + +   ++K +     K+PNAAVGQSGL ALYE MFRNYGILVGQVLVTK DF N++TREQLF TI EL+ LNIIP+INTNDAVSPPPQ  +  +G L I DNDSLA  +AV+  ADLAILMSDV+GIY++ P  E A+++  FNP   N++++G KS+ GTGGMESKV++A +ALE+G SVVIC+GMKYNTIR IM  + VG+ FT  E +   VE+ A+NAR GSRRL +LAP ERA+ I  ++NSLL+ Q EIM  N  DL  AR  GV G M+ RLA S  K++SL  GL QIA++S++NVGRV++RTKIS+T+ ++Q+TVPIGVLMVIFESRPDALPQ+A+LAIASANGLL+KGGKEATNSN +L+ +V EAL  YGCADAI++VS R  + +LLK+D YIDL+IPRGS ELV +IKE+SK IPVLGHA+GICHVY+D D D+EKA+ IV D+KTDYPAACNAMETLLIHE LL+  +F K+C  LK+  V I+SGP L++ LTFGPP+A  L  EYGD+ACT+EIV  L DAIDHIHK+GSSHTDVIVTEN++T+  FL +VDSACVF N SSR ADG+RLGLGAEVGISTGRIHARGPVGVEGLLTTKWVL G  DTA  Y+ G ++F+H+ LPL
Sbjct:   42 ALRLKTVLTVRSRLLSSLPPKNMLQRRWCTTTTG----RRYSANTLGLALSQTNSNNSFSQALLNMNNSVDHGMRHSPFLTRDELVNAKRVVIKLGSAVVTRADGQGLALGRLAAIIEQVSEIQNSGAECILVTSGAVAFGKQRLSQELLMSMSMRETLS-SVDRSSELKNIAQHELKRPNAAVGQSGLMALYEAMFRNYGILVGQVLVTKQDFRNENTREQLFNTIRELMALNIIPVINTNDAVSPPPQDGDLGDGTLNITDNDSLACTIAVDAGADLAILMSDVEGIYNKPPKEEGAQLLPYFNPKIVNQIQYGEKSNYGTGGMESKVKAACYALEHGCSVVICSGMKYNTIRNIMSAENVGTMFTPLEIEGTTVEIQAQNARKGSRRLSSLAPEERAEAIRHLSNSLLTHQAEIMEANNQDLANARAHGVTGPMFDRLALSSSKLESLCVGLNQIADSSYDNVGRVLKRTKISETLEVVQETVPIGVLMVIFESRPDALPQIAALAIASANGLLLKGGKEATNSNRVLIRLVNEALGRYGCADAITLVSGRNEVSELLKLDGYIDLIIPRGSNELVRSIKERSKSIPVLGHADGICHVYLDSDCDVEKAIKIVVDAKTDYPAACNAMETLLIHESLLENEVFYKVCQALKQENVKIYSGPRLSETLTFGPPKASKLSIEYGDLACTIEIVSTLDDAIDHIHKYGSSHTDVIVTENQDTADRFLNNVDSACVFHNVSSRFADGFRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLRGTDDTAEEYSKGRKEFIHKSLPL 839          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. nr
Match: gi|1325314463|ref|XP_023338908.1| (delta-1-pyrroline-5-carboxylate synthase-like isoform X3 [Eurytemora affinis])

HSP 1 Score: 1025 bits (2649), Expect = 0.000e+0
Identity = 499/803 (62.14%), Postives = 614/803 (76.46%), Query Frame = 0

Query:   63 AAKLSSATKRSNDLLRSFPPKRLFFSRGLTTDGGPSGVKKYGS-------------------LPMFSGSDTHSETKSAFLYRHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYASGERKFLHQQLPL 846
            A +L +     + LL S PPK +   R  TT  G    ++Y +                   L   + S  H    S FL R +L NAKR+VIK+GSAV+TR DG GLALGRLA+I+EQVSE+QN G ECIL+TSGAVAFGKQ+L+QE++MSMSMRET+S + +   ++K +     K+PNAAVGQSGL ALYE MFRNYGILVGQVLVTK DF N++TREQLF TI EL+ LNIIP+INTNDAVSPPPQ  +  +G L I DNDSLA  +AV+  ADLAILMSDV+GIY++ P  E A+++  FNP   N++++G KS+ GTGGMESKV++A +ALE+G SVVIC+GMKYNTIR IM  + VG+ FT  E +   VE+ A+NAR GSRRL +LAP ERA+ I  ++NSLL+ Q EIM  N  DL  AR  GV G M+ RLA S  K++SL  GL QIA++S++NVGRV++RTKIS+T+ ++Q+TVPIGVLMVIFESRPDALPQ+A+LAIASANGLL+KGGKEATNSN +L+ +V EAL  YGCADAI++VS R  + +LLK+D YIDL+IPRGS ELV +IKE+SK IPVLGHA+GICHVY+D D D+EKA+ IV D+KTDYPAACNAMETLLIHE LL+  +F K+C  LK+  V I+SGP L++ LTFGPP+A  L  EYGD+ACT+EIV  L DAIDHIHK+GSSHTDVIVTEN++T+  FL +VDSACVF N SSR ADG+RLGLGAEVGISTGRIHARGPVGVEGLLTTKWVL G  DTA  Y+ G ++F+H+ LPL
Sbjct:   28 ALRLKTVLTVRSRLLSSLPPKNMLQRRWCTTTTG----RRYSANTLGLALSQTNSNNSFSQALLNMNNSVDHGMRHSPFLTRDELVNAKRVVIKLGSAVVTRADGQGLALGRLAAIIEQVSEIQNSGAECILVTSGAVAFGKQRLSQELLMSMSMRETLS-SVDRSSELKNIAQHELKRPNAAVGQSGLMALYEAMFRNYGILVGQVLVTKQDFRNENTREQLFNTIRELMALNIIPVINTNDAVSPPPQDGDLGDGTLNITDNDSLACTIAVDAGADLAILMSDVEGIYNKPPKEEGAQLLPYFNPKIVNQIQYGEKSNYGTGGMESKVKAACYALEHGCSVVICSGMKYNTIRNIMSAENVGTMFTPLEIEGTTVEIQAQNARKGSRRLSSLAPEERAEAIRHLSNSLLTHQAEIMEANNQDLANARAHGVTGPMFDRLALSSSKLESLCVGLNQIADSSYDNVGRVLKRTKISETLEVVQETVPIGVLMVIFESRPDALPQIAALAIASANGLLLKGGKEATNSNRVLIRLVNEALGRYGCADAITLVSGRNEVSELLKLDGYIDLIIPRGSNELVRSIKERSKSIPVLGHADGICHVYLDSDCDVEKAIKIVVDAKTDYPAACNAMETLLIHESLLENEVFYKVCQALKQENVKIYSGPRLSETLTFGPPKASKLSIEYGDLACTIEIVSTLDDAIDHIHKYGSSHTDVIVTENQDTADRFLNNVDSACVFHNVSSRFADGFRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLRGTDDTAEEYSKGRKEFIHKSLPL 825          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. nr
Match: gi|1325314457|ref|XP_023338905.1| (delta-1-pyrroline-5-carboxylate synthase-like isoform X1 [Eurytemora affinis])

HSP 1 Score: 1025 bits (2649), Expect = 0.000e+0
Identity = 499/803 (62.14%), Postives = 614/803 (76.46%), Query Frame = 0

Query:   63 AAKLSSATKRSNDLLRSFPPKRLFFSRGLTTDGGPSGVKKYGS-------------------LPMFSGSDTHSETKSAFLYRHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYASGERKFLHQQLPL 846
            A +L +     + LL S PPK +   R  TT  G    ++Y +                   L   + S  H    S FL R +L NAKR+VIK+GSAV+TR DG GLALGRLA+I+EQVSE+QN G ECIL+TSGAVAFGKQ+L+QE++MSMSMRET+S + +   ++K +     K+PNAAVGQSGL ALYE MFRNYGILVGQVLVTK DF N++TREQLF TI EL+ LNIIP+INTNDAVSPPPQ  +  +G L I DNDSLA  +AV+  ADLAILMSDV+GIY++ P  E A+++  FNP   N++++G KS+ GTGGMESKV++A +ALE+G SVVIC+GMKYNTIR IM  + VG+ FT  E +   VE+ A+NAR GSRRL +LAP ERA+ I  ++NSLL+ Q EIM  N  DL  AR  GV G M+ RLA S  K++SL  GL QIA++S++NVGRV++RTKIS+T+ ++Q+TVPIGVLMVIFESRPDALPQ+A+LAIASANGLL+KGGKEATNSN +L+ +V EAL  YGCADAI++VS R  + +LLK+D YIDL+IPRGS ELV +IKE+SK IPVLGHA+GICHVY+D D D+EKA+ IV D+KTDYPAACNAMETLLIHE LL+  +F K+C  LK+  V I+SGP L++ LTFGPP+A  L  EYGD+ACT+EIV  L DAIDHIHK+GSSHTDVIVTEN++T+  FL +VDSACVF N SSR ADG+RLGLGAEVGISTGRIHARGPVGVEGLLTTKWVL G  DTA  Y+ G ++F+H+ LPL
Sbjct:   46 ALRLKTVLTVRSRLLSSLPPKNMLQRRWCTTTTG----RRYSANTLGLALSQTNSNNSFSQALLNMNNSVDHGMRHSPFLTRDELVNAKRVVIKLGSAVVTRADGQGLALGRLAAIIEQVSEIQNSGAECILVTSGAVAFGKQRLSQELLMSMSMRETLS-SVDRSSELKNIAQHELKRPNAAVGQSGLMALYEAMFRNYGILVGQVLVTKQDFRNENTREQLFNTIRELMALNIIPVINTNDAVSPPPQDGDLGDGTLNITDNDSLACTIAVDAGADLAILMSDVEGIYNKPPKEEGAQLLPYFNPKIVNQIQYGEKSNYGTGGMESKVKAACYALEHGCSVVICSGMKYNTIRNIMSAENVGTMFTPLEIEGTTVEIQAQNARKGSRRLSSLAPEERAEAIRHLSNSLLTHQAEIMEANNQDLANARAHGVTGPMFDRLALSSSKLESLCVGLNQIADSSYDNVGRVLKRTKISETLEVVQETVPIGVLMVIFESRPDALPQIAALAIASANGLLLKGGKEATNSNRVLIRLVNEALGRYGCADAITLVSGRNEVSELLKLDGYIDLIIPRGSNELVRSIKERSKSIPVLGHADGICHVYLDSDCDVEKAIKIVVDAKTDYPAACNAMETLLIHESLLENEVFYKVCQALKQENVKIYSGPRLSETLTFGPPKASKLSIEYGDLACTIEIVSTLDDAIDHIHKYGSSHTDVIVTENQDTADRFLNNVDSACVFHNVSSRFADGFRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLRGTDDTAEEYSKGRKEFIHKSLPL 843          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. nr
Match: gi|1325314465|ref|XP_023338909.1| (delta-1-pyrroline-5-carboxylate synthase-like isoform X4 [Eurytemora affinis])

HSP 1 Score: 1024.23 bits (2647), Expect = 0.000e+0
Identity = 499/803 (62.14%), Postives = 614/803 (76.46%), Query Frame = 0

Query:   63 AAKLSSATKRSNDLLRSFPPKRLFFSRGLTTDGGPSGVKKYGS-------------------LPMFSGSDTHSETKSAFLYRHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDEDPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKAEQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYASGERKFLHQQLPL 846
            A +L +     + LL S PPK +   R  TT  G    ++Y +                   L   + S  H    S FL R +L NAKR+VIK+GSAV+TR DG GLALGRLA+I+EQVSE+QN G ECIL+TSGAVAFGKQ+L+QE++MSMSMRET+S + +   ++K +     K+PNAAVGQSGL ALYE MFRNYGILVGQVLVTK DF N++TREQLF TI EL+ LNIIP+INTNDAVSPPPQ  +  +G L I DNDSLA  +AV+  ADLAILMSDV+GIY++ P  E A+++  FNP   N++++G KS+ GTGGMESKV++A +ALE+G SVVIC+GMKYNTIR IM  + VG+ FT  E +   VE+ A+NAR GSRRL +LAP ERA+ I  ++NSLL+ Q EIM  N  DL  AR  GV G M+ RLA S  K++SL  GL QIA++S++NVGRV++RTKIS+T+ ++Q+TVPIGVLMVIFESRPDALPQ+A+LAIASANGLL+KGGKEATNSN +L+ +V EAL  YGCADAI++VS R  + +LLK+D YIDL+IPRGS ELV +IKE+SK IPVLGHA+GICHVY+D D D+EKA+ IV D+KTDYPAACNAMETLLIHE LL+  +F K+C  LK+  V I+SGP L++ LTFGPP+A  L  EYGD+ACT+EIV  L DAIDHIHK+GSSHTDVIVTEN++T+  FL +VDSACVF N SSR ADG+RLGLGAEVGISTGRIHARGPVGVEGLLTTKWVL G  DTA  Y+ G ++F+H+ LPL
Sbjct:   11 ALRLKTVLTVRSRLLSSLPPKNMLQRRWCTTTTG----RRYSANTLGLALSQTNSNNSFSQALLNMNNSVDHGMRHSPFLTRDELVNAKRVVIKLGSAVVTRADGQGLALGRLAAIIEQVSEIQNSGAECILVTSGAVAFGKQRLSQELLMSMSMRETLS-SVDRSSELKNIAQHELKRPNAAVGQSGLMALYEAMFRNYGILVGQVLVTKQDFRNENTREQLFNTIRELMALNIIPVINTNDAVSPPPQDGDLGDGTLNITDNDSLACTIAVDAGADLAILMSDVEGIYNKPPKEEGAQLLPYFNPKIVNQIQYGEKSNYGTGGMESKVKAACYALEHGCSVVICSGMKYNTIRNIMSAENVGTMFTPLEIEGTTVEIQAQNARKGSRRLSSLAPEERAEAIRHLSNSLLTHQAEIMEANNQDLANARAHGVTGPMFDRLALSSSKLESLCVGLNQIADSSYDNVGRVLKRTKISETLEVVQETVPIGVLMVIFESRPDALPQIAALAIASANGLLLKGGKEATNSNRVLIRLVNEALGRYGCADAITLVSGRNEVSELLKLDGYIDLIIPRGSNELVRSIKERSKSIPVLGHADGICHVYLDSDCDVEKAIKIVVDAKTDYPAACNAMETLLIHESLLENEVFYKVCQALKQENVKIYSGPRLSETLTFGPPKASKLSIEYGDLACTIEIVSTLDDAIDHIHKYGSSHTDVIVTENQDTADRFLNNVDSACVFHNVSSRFADGFRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLRGTDDTAEEYSKGRKEFIHKSLPL 808          

BLAST of af512518_1pyrroline-5-carboxylase synthase vs. nr
Match: gi|1229878316|ref|XP_022183028.1| (delta-1-pyrroline-5-carboxylate synthase [Myzus persicae] >gi|1229878318|ref|XP_022183029.1| delta-1-pyrroline-5-carboxylate synthase [Myzus persicae] >gi|1229878320|ref|XP_022183030.1| delta-1-pyrroline-5-carboxylate synthase [Myzus persicae])

HSP 1 Score: 936.791 bits (2420), Expect = 0.000e+0
Identity = 451/744 (60.62%), Postives = 576/744 (77.42%), Query Frame = 0

Query:  109 FSGSDTHSETKSAFLYRHQLGNAKRIVIKMGSAVITREDGNGLALGRLASIVEQVSELQNEGRECILITSGAVAFGKQKLAQEVMMSMSMRETISGNYNTKEDIKTLMHQSFKKPNAAVGQSGLQALYETMFRNYGILVGQVLVTKPDFYNDDTREQLFTTINELLQLNIIPIINTNDAVSPPPQKDE----DPEGVLGIKDNDSLAARVAVETRADLAILMSDVDGIYDRSPSHEDARVMHTFNPADFNKVEFGVKSDLGTGGMESKVRSALWALENGSSVVICNGMKYNTIRKIMRGQKVGSFFTKA-EQDAMPVEVLAKNARAGSRRLQALAPSERAKIINSIANSLLSRQDEIMSVNELDLRQARLDGVVGAMYSRLAFSPQKIQSLATGLKQIAETSFNNVGRVVRRTKISDTMNLIQKTVPIGVLMVIFESRPDALPQVASLAIASANGLLMKGGKEATNSNILLMNIVKEALAEYGCADAISMVSNREAIGDLLKMDQYIDLVIPRGSGELVHTIKEQSKMIPVLGHAEGICHVYVDKDADLEKAVAIVKDSKTDYPAACNAMETLLIHEDLLKTPIFDKICATLKESGVAIFSGPALAQHLTFGPPQAHALKHEYGDMACTVEIVKGLYDAIDHIHKFGSSHTDVIVTENEETSQAFLESVDSACVFSNCSSRMADGYRLGLGAEVGISTGRIHARGPVGVEGLLTTKWVLTGQGDTAAAYAS-GERKFLHQQLPL 846
               +D+ S   S+F  R QL  A+R+VIK+GSAVITRED +G+ALGRLASIVEQV+E   EGRECI++TSGAVAFGKQKL  E++MS+SMRET+S N + ++D++TL+     +  AAVGQSGL +LY+ MF  YG+ + QVLVTKPDFYND+TR+ LF+T++EL+ +NI+PIINTNDAV+PPPQ D+      + V+ IKDNDSLAA +A E  ADL ILMSDVDGI+ R P  E A+++ T+N    + ++FG KS +GTGGM+SKV++A WAL  G SVVICNGM+   I+ I+ G+K+G+FFT A   +A PVE LA+NAR GSR LQ L   +RA  IN++A  L+S+Q  I+  NE D+  A  +G    + SRL+ +P K++SLA GLKQIAETS  NVG+V++RTKIS+ ++L Q TVPIGVL+VIFESRPD LPQVA+LA++SANGLL+KGGKEA++SN  LM+IVKEALA  G  DAIS+VS RE I DLL M+ +IDL+IPRGS +LV +I++Q   IPVLGHAEGICHVYVDKDA+L+KA+ I++DSK DYPAACNAMETLLIHEDL   P F+++C  LK+  V +FSGP L+  LTFGPP+A +LKHEYG++ACT+E+V G+  AI+HIH +GS HT+VI+TEN E +  F   VDSACVF N SSR ADG+R GLGAEVGIST RIHARGPVGVEGLLTTKW+L G G  A+ +A  G+R +LH+ LP+
Sbjct:   53 LQSADSQSVNGSSFADRSQLKYARRLVIKLGSAVITREDEHGIALGRLASIVEQVAECHQEGRECIMVTSGAVAFGKQKLTHELLMSLSMRETLSPNDHARKDVRTLLK---ARAAAAVGQSGLMSLYDAMFSQYGVKIAQVLVTKPDFYNDETRKNLFSTLSELISMNIVPIINTNDAVTPPPQDDDLIKSKGKKVISIKDNDSLAAMLAAEVSADLLILMSDVDGIFTRPPKQEGAKMIWTYNSDMADVIQFGKKSTVGTGGMDSKVQAATWALNRGVSVVICNGMQEKAIKSIVLGRKIGTFFTNAPTANATPVETLAENARYGSRILQKLPAVDRASAINALAELLISKQATILDANEKDIAIATKEGTAAPLISRLSLTPAKLKSLAIGLKQIAETSHTNVGKVLKRTKISENLDLTQVTVPIGVLLVIFESRPDCLPQVAALALSSANGLLLKGGKEASHSNKALMDIVKEALATVGAQDAISLVSTREEISDLLAMENHIDLIIPRGSSDLVRSIQDQCAHIPVLGHAEGICHVYVDKDANLDKALTIIRDSKCDYPAACNAMETLLIHEDLFGGPFFNQVCDMLKKENVELFSGPKLSSRLTFGPPRAKSLKHEYGELACTIELVSGMDSAINHIHTYGSGHTEVIITENTEAATKFQRDVDSACVFHNASSRFADGFRFGLGAEVGISTARIHARGPVGVEGLLTTKWILKGDGHAASEFAEGGDRTYLHEHLPV 793