BLAST of allergen aca s 13 vs. L. salmonis genes
Match: EMLSAG00000009662 (supercontig:LSalAtl2s:LSalAtl2s623:100839:101246:-1 gene:EMLSAG00000009662 transcript:EMLSAT00000009662 description:"augustus_masked-LSalAtl2s623-processed-gene-1.0")

HSP 1 Score: 44.2838 bits (103), Expect = 8.450e-7
Identity = 35/114 (30.70%), Postives = 53/114 (46.49%), Query Frame = 0

Query:   12 MSFEGKFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKPKGTLHFKGDCCQYKTVI---LGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLE-----IRREFS 117
            +S EG +K   ++NY+ FL  +   +  RK A      +TI +  +G  HFK +     T I   +GE+  E M  G   D     E DN L T ++ P + E     + R+FS
Sbjct:    2 VSIEGIYKRTKSDNYDAFLAKIGLNFLIRKAACLASATLTISKTSEGKWHFKTETTMRTTEIEFNVGEDWVETMSGGYKVDCSATLE-DNTL-TIIQKPQDAEGKMITLVRKFS 113          

BLAST of allergen aca s 13 vs. L. salmonis genes
Match: EMLSAG00000005888 (supercontig:LSalAtl2s:LSalAtl2s319:158357:158776:1 gene:EMLSAG00000005888 transcript:EMLSAT00000005888 description:"augustus_masked-LSalAtl2s319-processed-gene-1.4")

HSP 1 Score: 44.2838 bits (103), Expect = 8.868e-7
Identity = 35/138 (25.36%), Postives = 65/138 (47.10%), Query Frame = 0

Query:   14 FEGKFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEK-PKGTLHFKGDCCQYKTV----ILGEEHQEIMPTGESADAITVQEGDNCLITRLK----GPWNLEIRREFSPKCMTQHIRHTESDMETVRYFKRLT 142
            + GK++  + EN+E  L  L   +F RK A++  PI+ I  K  K T+    +    K++    + G   +E+ P G     +  +EG    IT  K    G  ++ + R+FS      ++  T  D+ + + FK+++
Sbjct:    5 YAGKYQRVSEENFESTLNELGLNFFLRKAALFSSPIMEISGKEDKWTI---ANSTLIKSIKMDFVFGVPFEEVSPDGRRCKTVVRREGLTRWITEQKALEEGKKDVVVIRDFSEDGKIINVMMTVGDVTSRQKFKKIS 139          

BLAST of allergen aca s 13 vs. SwissProt
Match: gi|14423698|sp|Q17284.1|FABP_BLOTA (RecName: Full=Fatty acid-binding protein; AltName: Full=Bt6; AltName: Allergen=Blo t 13)

HSP 1 Score: 53.5286 bits (127), Expect = 9.288e-9
Identity = 36/135 (26.67%), Postives = 65/135 (48.15%), Query Frame = 0

Query:   12 MSFEGKFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKP-----KGTLHFKGDCCQYKTVILGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRREFSPKCMTQHIRHTESDMETVRYFKRL 141
            M  EGK+K + ++N+++FL  L  G+  +  A    P + +D +      +    FK    ++K   LGEE +E    G+    +  +EGDN  I    G   ++I R+F    +   +  +  D+ +VR +KR+
Sbjct:    1 MPIEGKYKLEKSDNFDKFLDELGVGFMVKTAAKTLKPTLEVDVQGDTYVFRSLSTFKNTEIKFK---LGEEFEEDRADGKRVKTVVNKEGDNKFIQTQYGDKEVKIVRDFQGDDVV--VTASVGDVTSVRTYKRI 130          

BLAST of allergen aca s 13 vs. SwissProt
Match: gi|1169594|sp|P80425.1|FABPL_BOVIN (RecName: Full=Fatty acid-binding protein, liver; AltName: Full=Fatty acid-binding protein 1; AltName: Full=Liver-type fatty acid-binding protein; Short=L-FABP)

HSP 1 Score: 50.0618 bits (118), Expect = 1.743e-7
Identity = 45/137 (32.85%), Postives = 64/137 (46.72%), Query Frame = 0

Query:   12 MSFEGKFKYQANENYEEFLKLLNF--GWFKRKKAIYFCPIITIDEKPKGTLHFKGDCCQYKTVI-----LGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRREFSPKCMTQHIRHTESDMETVRYFKRL 141
            M+F GK++ Q  ENYE F+K +       ++ K I     I  + K     HFK        VI     LGEE +    TGE   A+  QEGDN L+T  KG  ++    EF+   +T  +  T+ D+   R  KR+
Sbjct:    1 MNFSGKYQVQTQENYEAFMKAVGMPDDIIQKGKDIKGVSEIVQNGK-----HFKFIITAGSKVIQNEFTLGEECEMEFMTGEKIKAVVQQEGDNKLVTTFKGIKSV---TEFNGDTVTSTM--TKGDVVFKRVSKRI 127          

BLAST of allergen aca s 13 vs. nr
Match: gi|118638266|gb|ABL09306.1| (allergen Aca s 13 [Acarus siro])

HSP 1 Score: 61.6178 bits (148), Expect = 2.029e-9
Identity = 40/135 (29.63%), Postives = 69/135 (51.11%), Query Frame = 0

Query:   12 MSFEGKFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKP-----KGTLHFKGDCCQYKTVILGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRREFSPKCMTQHIRHTESDMETVRYFKRL 141
            MS +GK+K + +EN++ FL  L  G+  +  A    P + ++ +      +    FK    ++K   LGEE +E    G+    + V+EGDN  +    G   ++I REF+   +T  +  T +D+ +VR +KRL
Sbjct:    1 MSIDGKYKLEKSENFDVFLDKLGVGFMVKTAAKTLKPTLEVETQGDTYIFRSLSTFKNTEIKFK---LGEEFEEDRADGKRVKTVVVKEGDNKFVQTQFGDKEVKIVREFAGDDVT--VTATVADVTSVRNYKRL 130          

BLAST of allergen aca s 13 vs. nr
Match: gi|146285347|gb|ABQ18251.1| (MPA13-like protein [Lygus lineolaris])

HSP 1 Score: 59.6918 bits (143), Expect = 1.385e-8
Identity = 34/112 (30.36%), Postives = 52/112 (46.43%), Query Frame = 0

Query:   14 FEG-KFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKPKGTLHFKGDCCQYKTVI---LGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRREFSPKCM 121
            F G K+K   +EN++E++K L  G   RK      P++ + +    +   K       T I   +GEE Q+  P G     +  Q+GDN L+   KG  +  I REFS   +
Sbjct:    5 FAGIKYKLDKSENFDEYMKALGVGMLTRKVGNSISPVVELVDNGDSSYTLKSSSTFKNTAITFKIGEEFQQETPDGRKVATVITQDGDNKLVEVQKGDKSTTIIREFSKDAV 116          

BLAST of allergen aca s 13 vs. nr
Match: gi|1059224311|ref|XP_017757721.1| (PREDICTED: fatty acid-binding protein 12-like isoform X2 [Eufriesea mexicana])

HSP 1 Score: 59.6918 bits (143), Expect = 1.573e-8
Identity = 43/142 (30.28%), Postives = 66/142 (46.48%), Query Frame = 0

Query:   13 SFEGKFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKPKGTLHFKGDCCQYKTV----------ILGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRR--EFSPKCMTQHIRHTESDMETVRYFKRLT 142
            S  G +++++NEN +E+ K +   +  RK      P + I           GD    +T+          IL EE++E MPTG     +T  EGDN L+T   GP N ++ R  E +   +   + H +S     RYFKRL+
Sbjct:    3 SIVGTYQHESNENLDEYFKAVGVPYIPRKMMCMSSPRLEISND--------GDKWTIRTITMIRTAEATFILNEEYEEHMPTGVVLKNVTTMEGDN-LVTVSVGPDNNKVIRKHELTQDGLVLTMTHEKSGQVAKRYFKRLS 135          

BLAST of allergen aca s 13 vs. nr
Match: gi|1279745428|ref|XP_022917754.1| (fatty acid-binding protein-like [Onthophagus taurus])

HSP 1 Score: 59.3066 bits (142), Expect = 1.904e-8
Identity = 39/129 (30.23%), Postives = 62/129 (48.06%), Query Frame = 0

Query:   16 GKFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKPKGTLHFKGDCCQYKTVI---LGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRR-EFSPKCMTQHIRHTESDMETVRYFKR 140
            GK+  ++NEN +E+ K +   +  RK      P +TI    KG             VI   LGEE+ E MP G +  ++T +EGD+ +           IRR +F+      +++H ES +E  R++KR
Sbjct:    6 GKYNLESNENLDEYYKAIGVPFIPRKMMCSSRPELTISVDEKGEWTISSSTLLRTVVITFRLGEEYNEHMPGGVTIQSVTTREGDSFVTVSTVPNGTKVIRRYDFNETHCILYLKHEESGVEAKRFYKR 134          

BLAST of allergen aca s 13 vs. nr
Match: gi|501290312|dbj|BAN20163.1| (allergen, putative [Riptortus pedestris])

HSP 1 Score: 58.151 bits (139), Expect = 4.577e-8
Identity = 39/128 (30.47%), Postives = 61/128 (47.66%), Query Frame = 0

Query:   17 KFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKPKGTLHFKGDCCQYKTVI---LGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRREFSPKCMTQHIRHTESDMETVRYFKRL 141
            K+K   +EN++EF+K +  G   RK      P++ + E   GT   K       T +   LGEE +E  P G    ++   +GDN LI   KG    +I REF+P  +   +  T  D+   R +K++
Sbjct:   11 KYKLDKSENFDEFMKAIGVGMIARKMGNTVSPVVELTEDG-GTYTLKSSSTFKDTELVFKLGEEFEEETPDGRKVKSLITLDGDNKLIHVQKGDKESKIVREFTPDEVKMTL--TVDDIVCTRIYKKV 135          

BLAST of allergen aca s 13 vs. nr
Match: gi|501290506|dbj|BAN20204.1| (allergen, putative [Riptortus pedestris])

HSP 1 Score: 58.151 bits (139), Expect = 4.675e-8
Identity = 36/105 (34.29%), Postives = 50/105 (47.62%), Query Frame = 0

Query:   17 KFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKPKGTLHFKGDCCQYKTVI---LGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRREFSP 118
            K+K   +E ++EF+K L  G   RK      P++ + E   GT   K       TV    LGEE +E  P G    ++   +GDN LI   KG    +I REF+P
Sbjct:   11 KYKLDKSEKFDEFMKALGVGLITRKMGNTVSPVVELTEDG-GTYTLKSSSTFKNTVTTFKLGEEFEEDTPDGRKVKSVITLDGDNKLIHVQKGDKESKIVREFTP 114          

BLAST of allergen aca s 13 vs. nr
Match: gi|1059224309|ref|XP_017757720.1| (PREDICTED: fatty acid-binding protein, brain-like isoform X1 [Eufriesea mexicana])

HSP 1 Score: 58.9214 bits (141), Expect = 8.863e-8
Identity = 43/142 (30.28%), Postives = 66/142 (46.48%), Query Frame = 0

Query:   13 SFEGKFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKPKGTLHFKGDCCQYKTV----------ILGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRR--EFSPKCMTQHIRHTESDMETVRYFKRLT 142
            S  G +++++NEN +E+ K +   +  RK      P + I           GD    +T+          IL EE++E MPTG     +T  EGDN L+T   GP N ++ R  E +   +   + H +S     RYFKRL+
Sbjct:   77 SIVGTYQHESNENLDEYFKAVGVPYIPRKMMCMSSPRLEISND--------GDKWTIRTITMIRTAEATFILNEEYEEHMPTGVVLKNVTTMEGDN-LVTVSVGPDNNKVIRKHELTQDGLVLTMTHEKSGQVAKRYFKRLS 209          

BLAST of allergen aca s 13 vs. nr
Match: gi|1061135885|ref|XP_017891258.1| (PREDICTED: probable fatty acid-binding protein [Ceratina calcarata])

HSP 1 Score: 56.6102 bits (135), Expect = 1.813e-7
Identity = 36/110 (32.73%), Postives = 53/110 (48.18%), Query Frame = 0

Query:   17 KFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKP-----KGTLHFKGDCCQYKTVILGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRREFSPKCM 121
            K+K  ++EN+++F+K L  G   RK      P++ + E       K T  FK    ++K   LGEE  E  P G    ++   +G N LI + KG     I REF+P  M
Sbjct:    8 KYKLSSSENFDDFMKALGVGMLTRKMGSSVSPVVELTENNGLYTLKTTSTFKSSEIKFK---LGEEFDEETPDGRKVKSVCTLDG-NKLIQKQKGDKETTIEREFTPSEM 113          

BLAST of allergen aca s 13 vs. nr
Match: gi|665809897|ref|XP_008553338.1| (PREDICTED: fatty acid-binding protein, liver isoform X1 [Microplitis demolitor])

HSP 1 Score: 56.225 bits (134), Expect = 3.064e-7
Identity = 38/138 (27.54%), Postives = 65/138 (47.10%), Query Frame = 0

Query:   16 GKFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKPKGTLHFKGDCCQYKTV----------ILGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRR-EFSPKCMTQHIRHTESDMETVRYFKRLT 142
            GK++++ +EN +E+ K LN  +  R K IY          P+  +  +GD    K +          +L  E +E+MP+G +    T  EGD  +I  +    N   RR +F    +   + H ++D+   RYFKR++
Sbjct:    6 GKYQHERSENLDEYFKTLNLPYLVR-KMIYIS-------NPRKEITKEGDKWIIKNITIMRTQVWEFLLDTEWEEVMPSGVTLKNTTTIEGDTLIIVSITEDGNKVTRRYDFDDNGIVLTMSHEKTDLVAKRYFKRIS 135          

BLAST of allergen aca s 13 vs. nr
Match: gi|805777275|ref|XP_012138207.1| (PREDICTED: fatty acid-binding protein, muscle isoform X2 [Megachile rotundata])

HSP 1 Score: 55.8398 bits (133), Expect = 4.445e-7
Identity = 37/110 (33.64%), Postives = 53/110 (48.18%), Query Frame = 0

Query:   17 KFKYQANENYEEFLKLLNFGWFKRKKAIYFCPIITIDEKP-----KGTLHFKGDCCQYKTVILGEEHQEIMPTGESADAITVQEGDNCLITRLKGPWNLEIRREFSPKCM 121
            ++K Q++EN++EF+K L  G   RK      P++ + E       K T  FK    ++K   LGEE  E  P G    ++   +G N LI   KG     I REF+P  M
Sbjct:    9 RYKLQSSENFDEFMKCLGVGLMTRKMGSSVSPVVELTENSGLYTLKTTSPFKNSEIKFK---LGEEFDEETPDGRKVKSVCTLDG-NKLIQIQKGDKQTTIEREFTPTEM 114