kunitz-like protease inhibitor precursor, maker-scaffold580_size130538-snap-gene-0.29 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000005801 (supercontig:LSalAtl2s:LSalAtl2s312:520364:527173:1 gene:EMLSAG00000005801 transcript:EMLSAT00000005801 description:"maker-LSalAtl2s312-augustus-gene-5.18") HSP 1 Score: 217.238 bits (552), Expect = 6.637e-60 Identity = 165/550 (30.00%), Postives = 247/550 (44.91%), Query Frame = 0
Query: 170 KLLRIESWPGRR--NNSFIVMNDLVVLQ-----------------TNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH-KPEKDDPCISPLLKG--SCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIF----------RVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCE-IQIAPRCRIEHTFEPCLPSPSY----TSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALE---GCLLRPERGS--CHGSLPRFYYEPMDETCHSFTFSGCH-GNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQP---RFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVAEIFIGIALSGLVILGVYFGFR 673
++LRIESW G N+ ++++D + Q T + + T STT +P+T S + PC L M GPC IR++++ +EKC F +GGCKGN N FK+ ++C + C K + D C P G CS E++ FD Q TC F+YSGCGGN NNF S EC++ C +D S+ + F + P R +CLKP G C + ++ +N CV +IY GCGG+DN F + + C+ C+ ++ +E +P T+ + Y + C GC + K G F + + C ++C S S V P + C GS C+ SL RFY++ TC F ++GC NGN F + C A C + S C PK G + D R++++ ETC F + GC G++NNF C +C N ++ I A + ST S VAE+ +G+ L L +G+ G +
Sbjct: 425 QILRIESWKGSALDNSEVVILDDFSLKQELLQHELPSSSTPKVPKTTKVTSLDELTTASTTINVPTTLLQEITSFKSDADDNFPCDLPMNSGPCRGNFIRYYFDTASEKCLEFSYGGCKGNANRFKAKKDCYSLCPHRDKSKAIDSCKLPRDPGDVKCSFYSERYFFDSQLGTCLIFLYSGCGGNSNNFKSRKECKNKCS--VDEDSRSIAKKTFLRLTGSVKRDKFPSR--QECLKPAEVGPCGGNYVAFAYDLNKNDCVRFIYGGCGGNDNRFYTIDKCRTVCQKKKVKNHLDLEDPCNDIIPKQKKKQHCTNRYFYDPRSKRCY--SGCPSLKVKG--FVSRASCFKSCAPASSVSTTVMPELLITPNVKCTNPLSTGSNLCNDSLIRFYFDQSTNTCEKFIYTGCGVRNGNIFPRKEVCAAVCRVP--------VGEGSPCYSPKDVGSSKCDAAKSIRYYFDFWKETCLPFEFLGCNGNKNNFVDFQSCNTSC-NFIINESIPSSAPDMSI----STISAPTVAEVVLGVILIILFTIGIVMGLK 953 HSP 2 Score: 51.6026 bits (122), Expect = 6.369e-7 Identity = 22/47 (46.81%), Postives = 27/47 (57.45%), Query Frame = 0
Query: 26 PERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMC 72
P C FY RYFF+ G C F +SGCG N NNF+ +K C+ C
Sbjct: 577 PGDVKCSFYSERYFFDSQLGTCLIFLYSGCGGNSNNFKSRKECKNKC 623
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000007668 (supercontig:LSalAtl2s:LSalAtl2s446:196154:280179:1 gene:EMLSAG00000007668 transcript:EMLSAT00000007668 description:"maker-LSalAtl2s446-snap-gene-2.16") HSP 1 Score: 183.726 bits (465), Expect = 2.748e-48 Identity = 140/487 (28.75%), Postives = 212/487 (43.53%), Query Frame = 0
Query: 179 GRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISP--------------LLK--GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD-CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQI----------APRCRIEHTFEPC-LPSPS---YTSV-WVY---SEEQRMCVVTVGCSNTKRIGNEFSTSSECERTC-----LSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC----HDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
G N+F +++ NQ +P+ D T LP + + + C+L+ G C L R++Y+ + KC F + GC GN NNFK+L EC++ C + + + P + P L K G C ++++D S C +F Y+GCGGN NNF +L EC+ C+ N L P R++ + C P +G C Y ++ +C+ + Y+GCGG+ N F + CKN CE Q P C LP S Y + W Y S + T C + N F T ECE C +SD ++L F ++ C L + G C ++ R+YY+ C F ++GC GN NNF+ Q C C HD N ++C K KG C + R++++ + C++F Y GC G+RNNF C+ C
Sbjct: 381 GGNQNNFKTISECKSYCKNQYYKPNI--HDEKVTPLPPSKDVNEV-----------CRLSKEAGHCYNLLWRYYYDSYSGKCLRFTYTGCGGNRNNFKTLPECKSYCQKQYYKPNIPLVKPRPLPPLRDVIDVCRLSKETGHCYDKLWRYYYDSYSGKCLRFTYTGCGGNQNNFKTLPECKRYCEKQYYKPHNPLVHSQSLPPSRNVINVCGLPKEAGHCYNLLWRYHYDSYSGKCLKFSYTGCGGNQNNFKTVTDCKNYCEKQYYKPPTHLVKPKPLTHPSGVINVCGLPKESGHCYNKLMWRYYYDSYYGKCLKFTFSCGGNQ---NNFKTLHECENYCKVKHYMSD--NTLVASNPFYVKSCHLPKKTGHCFRNIWRYYYDSTIGKCEKFIYTGCGGNLNNFERLQDCQKVCIAHVHDLPKVTPTPPEPNTNVCLLSKEKGHCFNNVWRYYFDKKTQKCQQFHYSGCGGNRNNFKIITDCKKRC 849 HSP 2 Score: 180.644 bits (457), Expect = 2.792e-47 Identity = 179/664 (26.96%), Postives = 274/664 (41.27%), Query Frame = 0
Query: 18 IPEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEE-EILRQEPRRSLKNLTLDFDQGFEDWDNYD--WQII--SYNSDLAR--------GLNLYSPDPG-SRSCA----GPLRPQGFSKSHPKLMHPIQV--DRNEAITITLSLKLLRIESWPGR-----------RNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPC-DLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCIS-----------PLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPS----YTSVWVY----SEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFA---LEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILT----NASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
+ E C L E GHC+ RY+++ SG C F+++GCG N NNF+ C+ C +++ P L+++ E YD W+ SY+ R N + P R C P P S+S P + I V EA L +S+ G+ N+F + D Q +P T P T P+ ++ C L G C + + R++Y+ KC F F C GN NNFK+L ECE C D+ ++ P G C + ++++D C KF+Y+GCGGN NNF+ L +CQ C H ++ L + P+ + + CL G C Y F++ +C + YSGCGG+ N F CK RCE+++ P + CL S + S+W Y S ++ + GC GN F +C+ C + V+P + C L E+G C+ + R+Y++ C F ++G GNGNNF+ C C + + T + + C PK KG C + R++Y+ +I C++F Y GC G+ NNF CE C +
Sbjct: 420 VNEVCRLSKEAGHCYNLLWRYYYDSYSGKCLRFTYTGCGGNRNNFKTLPECKSYCQKQYYKPNIPLVKPRPLPPLRDVIDVCRLSKETGHCYDKLWRYYYDSYSGKCLRFTYTGCGGNQNNFKTLPECKRYCEKQYYKPHNPLVHSQSLPPSRNVINVCGLPKEAGHCYNLLWRYHYDSYSGKCLKFSYTGCGGNQNNFKTVTDCKNYCEKQYYKPPT----HLVKPKPLTHPSGVINV---------CGLPKESGHCYNKLMWRYYYDSYYGKCLKFTF-SCGGNQNNFKTLHECENYCKVKHYMSDNTLVASNPFYVKSCHLPKKTGHCFRNIWRYYYDSTIGKCEKFIYTGCGGNLNNFERLQDCQKVCIAH----VHDLPKVTPTPPEPNTNVCLLSKEKGHCFNNVWRYYFDKKTQKCQQFHYSGCGGNRNNFKIITDCKKRCEVEVTPT-PPKQDINVCLLSKVRGNCHKSIWRYYFDKSTQKCRKFIYSGCGEN---GNNFKRIIDCKVRC------EVTVKPTTPNPNINVCFLSKEQGRCYKRIWRYYFDKKTHRCEKFVYTGXGGNGNNFKRXIDCNKRCDVQQTQPPIVTPTPPKSDYNSCYLPKEKGRCSKSIWRYYYDSNIGKCKKFIYTGCGGNLNNFERLQDCENVCTD 1055 HSP 3 Score: 174.096 bits (440), Expect = 3.765e-45 Identity = 133/467 (28.48%), Postives = 211/467 (45.18%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSE--HKPEKDDPCISPLL--------------KGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC-KDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ-IAPRCRIEH--TFEPC--------LPSPS---YTSVWVYSEEQR----MCVVTVGCSNTKRIGNEFSTSSECERTC----LSDRSHSLEVEPMFALEG----CLLRPERGSCHGSLP-RFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTN----ASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVAE 653
C+L+ G C L R++Y+ + KC F + GC GN NNFK++ EC++ C +KP D ++PL G C ++++D S C +F Y+GCGGN NNF +L EC+S C K + P + + + + C +G C + Y ++ +C+ + Y+GCGG+ N F + CK CE Q P + H + P LP + Y +W Y + + GC + N F T ++C+ C +H ++ +P+ G C L E G C+ L R+YY+ C FTFS C GN NNF++ C C +D +++ + C PK G C R+ R++Y+ +I CE+F Y GC G+ NNF C+ C+ A + L + + T E +T+ ++ E
Sbjct: 347 CRLSKETGHCYDKLWRYYYDSYSGKCIRFTYTGCGGNQNNFKTISECKSYCKNQYYKPNIHDEKVTPLPPSKDVNEVCRLSKEAGHCYNLLWRYYYDSYSGKCLRFTYTGCGGNRNNFKTLPECKSYCQKQYYKPNIPLVKPRPLPPLRDVIDVCRLSKETGHCYDKLWRYYYDSYSGKCLRFTYTGCGGNQNNFKTLPECKRYCEKQYYKPHNPLVHSQSLPPSRNVINVCGLPKEAGHCYNLLWRYHYDSYSGKCLKFSYTGCGGNQ---NNFKTVTDCKNYCEKQYYKPPTHLVKPKPLTHPSGVINVCGLPKESGHCYNKLMWRYYYDSYYGKCLKFTFS-CGGNQNNFKTLHECENYCKVKHYMSDNTLVASNPFYVKSCHLPKKTGHCFRNIWRYYYDSTIGKCEKFIYTGCGGNLNNFERLQDCQKVCI-----AHVHDLPKVTPTPPEPNTNVCLLSKE 804 HSP 4 Score: 166.007 bits (419), Expect = 1.596e-42 Identity = 170/661 (25.72%), Postives = 258/661 (39.03%), Query Frame = 0
Query: 19 PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCS-------------EHKPEKDD---PCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD-HMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ--------IAPR-----------CRIEHTFEPCLPSPSYTSVWVYSEEQR----MCVVTVGCSNTKRIGNEFSTSSECERTCLSDR-------SHSLEVEP-MFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSI-----LTNAS----ICEQPKLKGPC-RRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
P CLL G C Y RY+FN + +CE F +SGC N NNF+ K C C +L +P + + +D I+S +S L S R P PK++ V++ I L +P F ++ L T+ + P P L+ S G C L G C L R++++ + KC F++ GC GN NNFK+L C+ C +H P D C G C ++++D S C +F Y+GCGGN NNF +++EC+S CK+ + P ++ K C +G C Y ++ +C+ + Y+GCGG+ N F + CK+ C+ Q + PR CR+ C Y +W Y + + GC + N F T EC+R C HS + P + C L E G C+ L R++Y+ C F+++GC GN NNF++ C C + + LT+ S +C PK G C + R++Y+ C +F + C G++NNF T +CE C
Sbjct: 94 PPTCLLPKVTGPCEGYFERYYFNPDTDSCEGFIYSGCRGNANNFQSYKDCCVTC--------HVLSSKPEGKSIDFIEPSSKFLDDSSVAKNGIVSSHS-----LKFTSLVSQWRKTHSPF---------PKIL--AHVNKGPIIHFPL---------YPNIHRRPF--LHKLHFKPTHPQIHPRLPGYHPHL----HPKPKLVLAYGHSYTKGV-CHLPKKAGNCYNKLRRYYFDSYSGKCLKFIYTGCGGNQNNFKTLYMCKNHCKNRHYTVNIPLVKRKHLPSSRDVINVCRLSKETGHCYDKLWRYYYDSYSGKCIRFTYTGCGGNQNNFKTISECKSYCKNQYYKPNIHDEKVTPLPPSKDVNEVCRLSKEAGHCYNLLWRYYYDSYSGKCLRFTYTGCGGNRNNFKTLPECKSYCQKQYYKPNIPLVKPRPLPPLRDVIDVCRLSKETGHC-----YDKLWRYYYDSYSGKCLRFTYTGCGGNQ---NNFKTLPECKRYCEKQYYKPHNPLVHSQSLPPSRNVINVCGLPKEAGHCYNLLWRYHYDSYSGKCLKFSYTGCGGNQNNFKTVTDCKNYCEKQYYKPPTHLVKPKPLTHPSGVINVCGLPKESGHCYNKLMWRYYYDSYYGKCLKFTF-SCGGNQNNFKTLHECENYC 705 HSP 5 Score: 150.599 bits (379), Expect = 1.209e-37 Identity = 118/415 (28.43%), Postives = 186/415 (44.82%), Query Frame = 0
Query: 222 TLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHK---------PEKDD--PCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPC---LPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEP-MFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
T+ +T + C L+ +G C + R++++ KT +CE FV+ G GNGNNFK +C C + P K D C P KG CS S ++++D C KF+Y+GCGGN NNF+ L +C++ C DH+ + + P + T + + E+ + + +PY+ S + ++ +++ +I I C+ E T+ C L Y S+ E + GC+ N F + S C+R C S ++ + GC L E G C+ L R+Y++ C F ++GC GN NNF S++ C C GS +C K G C ++F+N CE+F Y GC G++NNF +C TC
Sbjct: 918 TVKPTTPNPNINVCFLSKEQGRCYKRIWRYYFDKKTHRCEKFVYTGXGGNGNNFKRXIDCNKRCDVQQTQPPIVTPTPPKSDYNSCYLPKEKGRCSKSIWRYYYDSNIGKCKKFIYTGCGGNLNNFERLQDCENVCTDHIPKY-----KEVTPTPPKVEYSLEDKDTGKNSEGEKSMV-------ELLPYLESL---HKIYYGGNKGGQHKSQITIDWACKFEKTYGTCSQELKRYFYNSITSKCES----FIYTGCAGNP---NNFLSQSRCDRICAKSISQKTVLQSGKYIFPGCYLGREVGYCNSYLERYYFDTNLGRCVQFIYTGCGGNTNNFNSYKKCNFLC-------GGSAERPIKVCRLEKNIGYCHDQIDKYFFNTHTRKCEKFIYSGCGGNKNNFDNETECNETC 1303 HSP 6 Score: 147.902 bits (372), Expect = 9.871e-37 Identity = 120/480 (25.00%), Postives = 197/480 (41.04%), Query Frame = 0
Query: 214 LPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC----SEHKPEKD-DPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ---------IAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC----VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPM-----FALE---------------------------------------------GCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASI----CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
LP TP + E + C L+ +G C + R++++ KT+KC+ F + GC GN NNFK + +C+ C + P++D + C+ ++G+C S +++FD + C KF+YSGCG NGNNF + +C+ C+ + PT P +++ C G C Y F++ ++C ++Y+G GG+ N F C RC++Q P+ + P S+W Y + + + GC N F +CE C EV P ++LE C G+C L R++Y + C SF ++GC GN NNF S C C A+ + ++L + C + G C R++++ ++ C +F Y GC G+ NNF + +C C
Sbjct: 784 LPKVTP------TPPEPNTNVCLLSKEKGHCFNNVWRYYFDKKTQKCQQFHYSGCGGNRNNFKIITDCKKRCEVEVTPTPPKQDINVCLLSKVRGNCHKSIWRYYFDKSTQKCRKFIYSGCGENGNNFKRIIDCKVRCEVTVKPT----------TPNPNINVCFLSKEQGRCYKRIWRYYFDKKTHRCEKFVYTGXGGNGNNFKRXIDCNKRCDVQQTQPPIVTPTPPKSDYNSCYLPKEKGRCSKSIWRYYYDSNIGKCKKFIYTGCGGNL---NNFERLQDCENVCTDHIPKYKEVTPTPPKVEYSLEDKDTGKNSEGEKSMVELLPYLESLHKIYYGGNKGGQHKSQITIDWACKFEKTYGTCSQELKRYFYNSITSKCESFIYTGCAGNPNNFLSQSRCDRIC--AKSISQKTVLQSGKYIFPGCYLGREVGYCNSYLERYYFDTNLGRCVQFIYTGCGGNTNNFNSYKKCNFLC 1242 HSP 7 Score: 105.145 bits (261), Expect = 2.501e-23 Identity = 90/357 (25.21%), Postives = 141/357 (39.50%), Query Frame = 0
Query: 208 DSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIE---HTFEPCLPS------PSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
D T P TP S S C L +G C + R++Y+ KC+ F++ GC GN NNF+ L++CE C++H P+ + +P K + + K G + + L +S K + N G+H ++ C T G C E Y +N ++C +IY+GC G+ N FLS C C I+ + ++ + F C SY + + CV + GC N F++ +C C ++V C L G CH + ++++ C F +SGC GN NNF + C TC
Sbjct: 982 DVQQTQPPIVTPTPPKSDYNS------CYLPKEKGRCSKSIWRYYYDSNIGKCKKFIYTGCGGNLNNFERLQDCENVCTDHIPKYKEVTPTP----------PKVEYSLEDKDTGK----NSEGEKSMVELLPYLESLHKIYY--GGNKGGQHKSQITIDWA--CKFEKTYGTCSQELKRYFYNSITSKCESFIYTGCAGNPNNFLSQSRCDRICAKSISQKTVLQSGKYIFPGCYLGREVGYCNSYLERYYFDTNLGRCVQFIYTGCGGNT---NNFNSYKKCNFLCGGSAERPIKV--------CRLEKNIGYCHDQIDKYFFNTHTRKCEKFIYSGCGGNKNNFDNETECNETC 1303 HSP 8 Score: 92.8189 bits (229), Expect = 1.630e-19 Identity = 47/136 (34.56%), Postives = 68/136 (50.00%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLS 370
C L G C+ YL R++++ +C F++ GC GN NNF S ++C C C G C +K+ F+ + C KF+YSGCGGN NNFD+ EC TC +N G + VP++ L+
Sbjct: 1192 CYLGREVGYCNSYLERYYFDTNLGRCVQFIYTGCGGNTNNFNSYKKCNFLCGGSAERPIKVCRLEKNIGYCHDQIDKYFFNTHTRKCEKFIYSGCGGNKNNFDNETECNETCTQDKHGLINEPGHGLISVPEKGLN 1327 HSP 9 Score: 89.7373 bits (221), Expect = 1.497e-18 Identity = 46/130 (35.38%), Postives = 74/130 (56.92%), Query Frame = 0
Query: 226 STSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC-------SEHKPEKDDP--CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
+ ++ + C A+ G C+ ++++N +T +CE FV+GGCKG+ N F SL +C+T C S ++ P C+ P + G C G E+++F+ + +C F+YSGC GN NNF S +C TC
Sbjct: 18 AATKTSSPKCNEALDIGECNQNETKYYFNNETNECEEFVYGGCKGSENRFDSLSDCKTLCESKDQNSSAYRQWGSYPPTCLLPKVTGPCEGYFERYYFNPDTDSCEGFIYSGCRGNANNFQSYKDCCVTC 147 HSP 10 Score: 77.411 bits (189), Expect = 8.873e-15 Identity = 65/222 (29.28%), Postives = 97/222 (43.69%), Query Frame = 0
Query: 303 GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC-KDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC------EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGS 515
G+CS +++ ++ + C F+Y+GC GN NNF S + C C K T+ G++IF C G C Y F+ +CV +IY+GCGG+ N F S++ C C I++ CR+E C + ++ R C + GC K N F +EC TC D+ H L EP L + PE+G G+
Sbjct: 1129 GTCSQELKRYFYNSITSKCESFIYTGCAGNPNNFLSQSRCDRICAKSISQKTVLQSGKYIF-------PGCYLGREVGYCNSYLERYYFDTNLGRCVQFIYTGCGGNTNNFNSYKKCNFLCGGSAERPIKV---CRLEKNIGYCHDQ---IDKYFFNTHTRKCEKFIYSGCGGNK---NNFDNETECNETCTQDK-HGLINEPGHGL---ISVPEKGLNSGT 1330 HSP 11 Score: 73.9442 bits (180), Expect = 1.175e-13 Identity = 42/148 (28.38%), Postives = 67/148 (45.27%), Query Frame = 0
Query: 510 GSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCH--DAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL---NREVDAFIQFLAEESETFHEESTSSGVVVA 652
G C+ + ++Y+ C F + GC G+ N F S C C D A + C PK+ GPC R+++NP ++CE F Y GC+G+ NNF + C TC ++ I F+ S+ + S + +V+
Sbjct: 34 GECNQNETKYYFNNETNECEEFVYGGCKGSENRFDSLSDCKTLCESKDQNSSAYRQWGSYPPTCLLPKVTGPCEGYFERYYFNPDTDSCEGFIYSGCRGNANNFQSYKDCCVTCHVLSSKPEGKSIDFIEPSSKFLDDSSVAKNGIVS 181 HSP 12 Score: 71.633 bits (174), Expect = 6.349e-13 Identity = 37/131 (28.24%), Postives = 63/131 (48.09%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNA---------SICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNR 624
C L + G+C+ L R+Y++ C F ++GC GN NNF++ C C + + ++ ++C K G C R++Y+ C F Y GC G++NNF T ++C++ C N+
Sbjct: 270 CHLPKKAGNCYNKLRRYYFDSYSGKCLKFIYTGCGGNQNNFKTLYMCKNHCKNRHYTVNIPLVKRKHLPSSRDVINVCRLSKETGHCYDKLWRYYYDSYSGKCIRFTYTGCGGNQNNFKTISECKSYCKNQ 400 HSP 13 Score: 70.4774 bits (171), Expect = 1.203e-12 Identity = 43/131 (32.82%), Postives = 66/131 (50.38%), Query Frame = 0
Query: 296 CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC--KDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEI 424
C L G C+ + K++F+ +++ C +FVY GC G+ N FDSL++C++ C KD G + CL P +G C+ Y FN + C +IYSGC G+ N F S++ C C +
Sbjct: 27 CNEALDIGECNQNETKYYFNNETNECEEFVYGGCKGSENRFDSLSDCKTLCESKDQNSSAYRQWGSYP--------PTCLLPKVTGPCEGYFERYYFNPDTDSCEGFIYSGCRGNANNFQSYKDCCVTCHV 149 HSP 14 Score: 66.6254 bits (161), Expect = 2.119e-11 Identity = 39/120 (32.50%), Postives = 60/120 (50.00%), Query Frame = 0
Query: 449 WVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLS-DRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSIL 565
+ ++ E C V GC ++ N F + S+C+ C S D++ S + CLL G C G R+Y+ P ++C F +SGC GN NNFQS++ C TCH + +G +
Sbjct: 43 YYFNNETNECEEFVYGGCKGSE---NRFDSLSDCKTLCESKDQNSSAYRQWGSYPPTCLLPKVTGPCEGYFERYYFNPDTDSCEGFIYSGCRGNANNFQSYKDCCVTCHVLSSKPEGKSI 159 HSP 15 Score: 59.6918 bits (143), Expect = 2.656e-9 Identity = 76/325 (23.38%), Postives = 111/325 (34.15%), Query Frame = 0
Query: 366 KRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ------------IAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTC--LSDR----------------------------SHSLEVEPMFA--------LEGCLLRPERGS-CHGSL-PRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEE 636
K S C + + G+C + Y FN N+C ++Y GC GS+N F S CK CE + P C + PC Y + ++ + C + GC N F + +C TC LS + SHSL+ + + L +G H L P + P H F H + H V A G T +C PK G C R++++ C +F Y GC G++NNF T C+ C NR I + +
Sbjct: 21 KTSSPKCNEALDIGECNQNETKYYFNNETNECEEFVYGGCKGSENRFDSLSDCKTLCESKDQNSSAYRQWGSYPPTCLLPKVTGPC---EGYFERYYFNPDTDSCEGFIYSGCRGN---ANNFQSYKDCCVTCHVLSSKPEGKSIDFIEPSSKFLDDSSVAKNGIVSSHSLKFTSLVSQWRKTHSPFPKILAHVNKGPIIHFPLYPNIHRRPF---LHKLHFKPTHPQIHPRLPGYHPHLHPKPKLVLAYGHSYTKG-VCHLPKKAGNCYNKLRRYYFDSYSGKCLKFIYTGCGGNQNNFKTLYMCKNHCKNRHYTVNIPLVKRK 335 HSP 16 Score: 53.1434 bits (126), Expect = 2.461e-7 Identity = 27/79 (34.18%), Postives = 40/79 (50.63%), Query Frame = 0
Query: 211 TTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHK 289
T S + L ++E C+L G C + ++F+N T KCE F++ GC GN NNF + EC TC++ K
Sbjct: 1229 TNNFNSYKKCNFLCGGSAERPIKVCRLEKNIGYCHDQIDKYFFNTHTRKCEKFIYSGCGGNKNNFDNETECNETCTQDK 1307
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000007907 (supercontig:LSalAtl2s:LSalAtl2s467:26502:36182:-1 gene:EMLSAG00000007907 transcript:EMLSAT00000007907 description:"maker-LSalAtl2s467-augustus-gene-0.15") HSP 1 Score: 150.984 bits (380), Expect = 1.038e-37 Identity = 112/394 (28.43%), Postives = 173/394 (43.91%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCL--KPITSGDCQ--LEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
C L GPC L +++++ ++C F +GGC GN N F LE C+ +C + + D C P + G+C E+F+FD F Y GC GN NNF S+ CQS C S+ I + L CL K + D Q EQ + ++ ++ C + G G+ N FL+ + C+ C + C + PC + S W + E++ C GC GN F + CE C D + ++ C L + G C +P +Y+ + C +F +SGC GN N F + + C C I ++C GPC +++++ TC EF +GGC+G+ N F + +CE CL
Sbjct: 1269 CLLPRDPGPCKDNLPKWYFDNFEKRCVPFYYGGCDGNDNKFDDLESCQKSCPKEFLQAD-VCKLPQVPGTCGDYLERFYFDIDEGVXKPFYYGGCDGNKNNFKSMENCQSRC---------SVDYSIPIAQEFKLEFCLLSKDVGVVDEQNPKEQNRWYYDSSDGVCKQMQFKGRRGNGNRFLTRQDCEASCR-ESQNICTLPKVRGPC--NSQIDSFW-FDEDKNSCFKFTWGGCQGN---GNRFESMEFCEAKC-KDTGSGAPSKEKANVDICNLPIDSGPCTDRIPNWYFNTKNGKCSAFIYSGCEGNANRFITQEQCDRNC---------GIFKGKNVCGSQMDIGPCVGRFKKWYFDLHTRTCHEFTFGGCEGNGNRFSSLGECETVCL 1635 HSP 2 Score: 147.132 bits (370), Expect = 1.533e-36 Identity = 113/397 (28.46%), Postives = 159/397 (40.05%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPS----------PSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C L +GPC+ Y R+ YN G N + C+ P G C + K++FD C F Y GC GN N FD L CQ +C L + ++P+ P T GD LE+ + F+ E P+ Y GC G+ N F S E C++RC + + E E CL S P + W Y +C + + GN F T +CE +C ++ C L RG C+ + F+++ +C FT+ GC GNGN F+S + C A C D A N IC P GPC P +++N C F Y GC+G+ N F T+ QC+ C
Sbjct: 1241 CVLPEVKGPCEGYYPRYSYN---------------SEGFNRQM------------------CLLPRDPGPCKDNLPKWYFDNFEKRCVPFYYGGCDGNDNKFDDLESCQKSCPKEF------LQADVCKLPQ-------VPGTCGD-YLER--FYFDIDEGVXKPFYYGGCDGNKNNFKSMENCQSRCSVDYSIPIAQEFKLEFCLLSKDVGVVDEQNPKEQNRWYYDSSDGVCK-QMQFKGRRGNGNRFLTRQDCEASCRESQNI------------CTLPKVRGPCNSQIDSFWFDEDKNSCFKFTWGGCQGNGNRFESMEFCEAKCKDTGSGAPSKEKANVDICNLPIDSGPCTDRIPNWYFNTKNGKCSAFIYSGCEGNANRFITQEQCDRNC 1575 HSP 3 Score: 139.428 bits (350), Expect = 4.441e-34 Identity = 107/417 (25.66%), Postives = 172/417 (41.25%), Query Frame = 0
Query: 230 LAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH------KPEKDDPCISPLLKGSCSGSGEK----FHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC----------EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC---HDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
L C+L G C YL RF+++ + F +GGC GN NNFKS+E C++ CS + K + C+ G K +++D C + + G GNGN F + +C+++C++ S + C P G C + + F++ +N C + + GC G+ N F S E C+ +C E C + PC W ++ + C + GC N F T +C+R C + ++ C + + G C G ++Y++ TCH FTF GC GNGN F S C C + ++ + + ++ + IC +G C R+++N C F Y GC G+RN F + C C
Sbjct: 1324 LQADVCKLPQVPGTCGDYLERFYFDIDEGVXKPFYYGGCDGNKNNFKSMENCQSRCSVDYSIPIAQEFKLEFCLLSKDVGVVDEQNPKEQNRWYYDSSDGVCKQMQFKGRRGNGNRFLTRQDCEASCRE-------------------SQNICTLPKVRGPCNSQIDSFWFDEDKNSCFKFTWGGCQGNGNRFESMEFCEAKCKDTGSGAPSKEKANVDICNLPIDSGPCT---DRIPNWYFNTKNGKCSAFIYSGCEGN---ANRFITQEQCDRNCGIFKGKNV----------CGSQMDIGPCVGRFKKWYFDLHTRTCHEFTFGGCEGNGNRFSSLGECETVCLVQDELPIQGNNTEVSRSEICMLQLDEGSCDDQLKRWYFNEERNICAPFLYTGCAGNRNRFKSYDICMGFC 1705 HSP 4 Score: 80.8777 bits (198), Expect = 9.671e-16 Identity = 43/132 (32.58%), Postives = 68/132 (51.52%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC----------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNS 356
C M GPC +++++ T C F FGGC+GNGN F SL ECET C + + + + C+ L +GSC ++++F+ + + C+ F+Y+GC GN N F S C C P+ ++
Sbjct: 1584 CGSQMDIGPCVGRFKKWYFDLHTRTCHEFTFGGCEGNGNRFSSLGECETVCLVQDELPIQGNNTEVSRSEICMLQLDEGSCDDQLKRWYFNEERNICAPFLYTGCAGNRNRFKSYDICMGFCGPKNPPSKDN 1715
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000011000 (supercontig:LSalAtl2s:LSalAtl2s73:722677:726549:1 gene:EMLSAG00000011000 transcript:EMLSAT00000011000 description:"maker-LSalAtl2s73-augustus-gene-7.20") HSP 1 Score: 142.51 bits (358), Expect = 9.162e-37 Identity = 99/349 (28.37%), Postives = 155/349 (44.41%), Query Frame = 0
Query: 208 DSTTTTLPSTTPA-STLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP--EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
DS+TT S S S T + +PC LA GPC ++RF YN ++ C+ F++GGC GN NNF+++E C+ C P +D PC P +G C G+ ++++F+ C F++ GC N NNF SL +C C D + ++ + C PI G+C+ YGF+ +C+ + YSGC G++N F S E CK C S S+ +++ K++G E ++ + L C + GSC G R+Y++ + C +F + GC GN NN++S + C C
Sbjct: 140 DSSTTPFESVEDCNSQCISPTYAVNKNPCTLAPDPGPCRGSMVRFHYNHESRLCDKFIYGGCVGNENNFRTVESCQKKCDVPLPISLEDPPCSIPPYRGPCRGNIQRWYFNNVKKICETFIFGGCLSNKNNFLSLEDCNDKC-DQRSGSPSTANK------------CFLPIDVGNCKASLPRYGFDVGSKKCIVFNYSGCEGNNNRFESLEDCKRNC-------------------SGSFLDTEGDNQDDD--------EEEKKVGEEITSKT---------------------LVDCTSDADSGSCKGMFMRYYFD--GKQCSTFIYGGCLGNNNNYKSAEKCKEAC 425 HSP 2 Score: 105.916 bits (263), Expect = 1.719e-24 Identity = 91/346 (26.30%), Postives = 152/346 (43.93%), Query Frame = 0
Query: 301 LKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIA-----PRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC-------------HDAEVRADGSILTNASI--CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNR 624
L +C G+ +++F + TC S C + F+S+ +C S C + PT + V K + P G C+ + +N C +IY GC G++N F + E+C+ +C++ + P C I PC + W ++ +++C + GC + K N F + +C C RS S P A C L + G+C SLPR+ ++ + C F +SGC GN N F+S + C C D E + G +T+ ++ C G C+ R++++ + C F YGGC G+ NN+ + +C+ C R
Sbjct: 111 LPKNCKGTNSQWYFAEEEGTCVIRNLSECDSSTTPFESVEDCNSQC---ISPT--------YAVNKNPCTLAPDP---GPCRGSMVRFHYNHESRLCDKFIYGGCVGNENNFRTVESCQKKCDVPLPISLEDPPCSIPPYRGPCRGN---IQRWYFNNVKKICETFIFGGCLSNK---NNFLSLEDCNDKC-DQRSGS----PSTA-NKCFLPIDVGNCKASLPRYGFDVGSKKCIVFNYSGCEGNNNRFESLEDCKRNCSGSFLDTEGDNQDDDEEEKKVGEEITSKTLVDCTSDADSGSCKGMFMRYYFDG--KQCSTFIYGGCLGNNNNYKSAEKCKEACSGR 428 HSP 3 Score: 96.2857 bits (238), Expect = 2.636e-21 Identity = 91/293 (31.06%), Postives = 130/293 (44.37%), Query Frame = 0
Query: 378 SGDCQLEQGVYG------FNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCL----PSPSYTSV--WVYSEEQRMC--VVTVGCSNTKRIGNE--FSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC----LNREVDAFIQFLAEESETFHEESTSSGVV 650
+G C+L + G F + E CV S C S F S E C ++C I+P + PC P P S+ + Y+ E R+C + GC +GNE F T C++ C SLE P C + P RG C G++ R+Y+ + + C +F F GC N NNF S + C C + GS T A+ C P G C+ PR+ ++ + C F Y GC+G+ N F + C+ C L+ E D Q EE + EE TS +V
Sbjct: 106 NGKCKLPKNCKGTNSQWYFAEEEGTCVIRNLSECDSSTTPFESVEDCNSQC---ISPTYAVNKN--PCTLAPDPGPCRGSMVRFHYNHESRLCDKFIYGGC-----VGNENNFRTVESCQKKCDVPLPISLEDPP------CSIPPYRGPCRGNIQRWYFNNVKKICETFIFGGCLSNKNNFLSLEDCNDKCD----QRSGSPST-ANKCFLPIDVGNCKASLPRYGFDVGSKKCIVFNYSGCEGNNNRFESLEDCKRNCSGSFLDTEGDN--QDDDEEEKKVGEEITSKTLV 375
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000009168 (supercontig:LSalAtl2s:LSalAtl2s579:234046:258823:-1 gene:EMLSAG00000009168 transcript:EMLSAT00000009168 description:"maker-LSalAtl2s579-augustus-gene-2.12") HSP 1 Score: 145.591 bits (366), Expect = 4.357e-36 Identity = 127/443 (28.67%), Postives = 197/443 (44.47%), Query Frame = 0
Query: 232 GSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC---------SEHKPE-KDDPCISPLLKGS--CSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNS-----LGRHIFRVP----KRSLSDCLKPITSG----DCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLS-----------HEACKNRCEIQIAPRCRIEH---TFEPCLPSP----SYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRP---ERG-SCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
G+ C GPC + LI ++YN T++CE+F FGGC+GN NNFKSL +C+ C SE P K + C S + G C+G +KF F++ + TC +VY GCG N +D+L EC S+C PT + G+ ++P + + D L P+ G + +E+ Y ++ N CV + +S E+ +++ I I P+ + E P P +Y W Y +++ CV + GC TK N F++ ++CE +C S S + + C L E G C + + +C + GC N F S Q C + C E +GS +C P G CR +P++ +N + CE+F Y GC+G+ N F + +C TC E
Sbjct: 114 GNKCSQPKETGPCRMILIAYYYNANTDRCESFYFGGCRGNDNNFKSLADCQKECHSSSYNPSSSELNPSVKSEHCKSDPIAGQMFCAGYFDKFTFNYDTLTCEPYVYGGCGATANLYDTLQECMSSCVHGSAPTGGAPMESRQGKEAIKLPHWKEEEDICD-LPPVHRGPITCEAYIEKWTYD-KKSGNSCVHGQVGASVTEKTIPVSFFRSAPTGGAPMESRQDKDAI-IFPQWKEEEDICDLPPVHKGPIMCAAYIEKWTYDKKRGKCVQFIHGGCFGTK---NNFASKADCENSCPKTPSRSTKPPQFIRPDFCSLPAVDNEGGIQCLALSYGYTFNGAKGSCEQIVYGGCGATANYFSSLQKCKSAC---EGEGNGS-QKFIDVCTLPTEAGKCRARKPKYTFNQETQRCEKFFYTGCEGNGNGFDSVEECFDTCGGAE 546 HSP 2 Score: 134.42 bits (337), Expect = 1.452e-32 Identity = 117/436 (26.83%), Postives = 188/436 (43.12%), Query Frame = 0
Query: 250 RFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKD----------DPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMN-SLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSV--WVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSD------RSHSLEV-EPMF--------ALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCH---DAEVRA----------DGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHE 642
R+ Y+ ++CE + G CK N F + +ECE C + P D C ++ G+C +++ + C F Y+GC GN NNF + +C+ C + + + + + + C +P SG C+ + Y F++ E C ++Y GC G+ N F + + C CE+ I + + + P P + + W Y ++ C + GC+ GN+F + ECE TC SD R + P+ ++ CLL G C +L +FY++ + C F + GC+GNGNNF S C C VR+ +G + + C P G CR + R +N + CE+F YGGC G+ NNF + +C TCL V + + HE
Sbjct: 930 RYRYDLSKDRCEPYTTGLCKPGPNRFSTQQECELKCVQSPPRIDLTKDNIPGLLPKCNLNVIPGNCRSRIKRYFYSPAHGGCRFFYYTGCQGNENNFLTAEKCEKACVPSTEVRSSFQVATEPNEIVEEIKNPCDQPKNSGLCKAMKPSYYFDKDEGVCKFFLYGGCMGNANRFFTLKECSLTCEVTIEKQSEGNYCKKAPEPGPCFAIIRKWYYDPKENDCKLFFYGGCAGN---GNKFDSKQECELTCSSDFDIPQGRIKDKNIPNPVIDDSILSTGMVDPCLLDKTIGDCRAALNKFYFDKESKECKLFKYGGCNGNGNNFPSEDICKQVCSRHIQVPVRSNEPPKTVTTEEGDEVAFSPNCFSPVDVGVCRGSKIRHHFNAASGLCEKFIYGGCLGNENNFLSEEECYDTCLPNLVHSQTDLKHKVDAKIHE 1362 HSP 3 Score: 126.716 bits (317), Expect = 4.591e-30 Identity = 133/517 (25.73%), Postives = 208/517 (40.23%), Query Frame = 0
Query: 191 LVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNG---NNFKSLEECETTCSEHKPEKDDP------------CISPLLKGSCSGS-----GEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC----------EIQIAPRCRIEHTFEPCLPSPSYTSV-------WVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC-----------HDAEVR---ADGSILTNASI--CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVA 652
+V ++ +SL+ + + ++ +PAS + S C L A GPC + +FF+N KC F +GG + N FKS ECE C P K+ P C++PL G SG ++ +D C + C N F + EC+ C P ++ +I + L C + G+C+ Y ++ C + Y+GC G++N FL+ E C+ C ++ P +E PC P + + + + +++ +C + GC N F T EC TC + +E C PE G C + ++YY+P + C F + GC GNGN F S Q C TC D + D SIL+ + C K G CR +F+++ + C+ F+YGGC G+ NNFP+ C+ C IQ +E +T G VA
Sbjct: 794 VVQMRIPKSLQ-NMMKNAPLDSSFQKHSPASVGAQGKSH---DDCLLPPAVGPCKGFRDKFFFNIDNRKCTAFKYGGFDEDAQGFNVFKSKGECEKVCERLLPSKEIPKPVLQQQQVDSECVTPLHHGITSGQLCLPLXRRYRYDLSKDRCEPYTTGLCKPGPNRFSTQQECELKCV-QSPPRIDLTKDNIPGL----LPKCNLNVIPGNCRSRIKRYFYSPAHGGCRFFYYTGCQGNENNFLTAEKCEKACVPSTEVRSSFQVATEPNEIVEEIKNPC-DQPKNSGLCKAMKPSYYFDKDEGVCKFFLYGGCMGN---ANRFFTLKECSLTC------EVTIEKQSEGNYCKKAPEPGPCFAIIRKWYYDPKENDCKLFFYGGCAGNGNKFDSKQECELTCSSDFDIPQGRIKDKNIPNPVIDDSILSTGMVDPCLLDKTIGDCRAALNKFYFDKESKECKLFKYGGCNGNGNNFPSEDICKQVCSRH-----IQVPVRSNEPPKTVTTEEGDEVA 1286 HSP 4 Score: 107.071 bits (266), Expect = 6.665e-24 Identity = 133/564 (23.58%), Postives = 205/564 (36.35%), Query Frame = 0
Query: 22 CLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNE---NNFRYKKACERMC---MNGNTTEEEILRQEPRRS-----------LKNLTLDFDQGFEDWDNYDWQIISYNSDLAR-GLNLYSPDPGS--RSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC--SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD----------CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
CLL P G C + ++FFN + C F + G + N F+ K CE++C + + +L+Q+ S L L + + +D + Y + L + G N +S + P R + P L+ ++ I ++ R P F + N +T + S+ +T + E +PC G C ++++ C+ F++GGC GN N F +L+EC TC + K + + C G C K+++D + + C F Y GC GNGN FDS EC+ TC D + +P + D CL T GDC+ + F++ +C + Y GC G+ N F S + CK C I V V S E V T G+E + S C P+ + G C GS R ++ C F + GC GN NNF S + C TC
Sbjct: 834 CLLPPAVGPCKGFRDKFFFNIDNRKCTAFKYGGFDEDAQGFNVFKSKGECEKVCERLLPSKEIPKPVLQQQQVDSECVTPLHHGITSGQLCLPLXRRYR-YDLSKDRCEPYTTGLCKPGPNRFSTQQECELKCVQSPPRIDLTKDNIPGLLPKCNLN---VIPGNCRSRIKRYFYSPAHGGCRFFYYTGCQGNENNFLTAEKCEKACVPSTEVRSSFQVATEPNEIVEEIKNPCDQPKNSGLCKAMKPSYYFDKDEGVCKFFLYGGCMGNANRFFTLKECSLTCEVTIEKQSEGNYCKKAPEPGPCFAIIRKWYYDPKENDCKLFFYGGCAGNGNKFDSKQECELTCSSDFDIPQGRIKDK--NIPNPVIDDSILSTGMVDPCLLDKTIGDCRAALNKFYFDKESKECKLFKYGGCNGNGNNFPSEDICKQVCSRHI--------------------QVPVRSNEPPKTVTT-------EEGDEVAFSPNC-------------FSPV----------DVGVCRGSKIRHHFNAASGLCEKFIYGGCLGNENNFLSEEECYDTC 1341 HSP 5 Score: 94.3597 bits (233), Expect = 4.784e-20 Identity = 100/441 (22.68%), Postives = 156/441 (35.37%), Query Frame = 0
Query: 244 CDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH----------------KPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC--------KDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-------------------------CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVV--TVGCSNT-KRIGNEFSTSSECERTCLSDRSHSLEVE-PMFALEG--CLLRPERGS---CHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSI-LTNASICEQPKLKG----PCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C Y +F + + T CE +++GGC N + +L+EC ++C + C P G C ++++ + C F + GC GN NNF SLA+CQ C ++P++ S H P C + FN C PY+Y GCG + NL+ + + C + C AP C + + +Y W Y ++ V VG S T K I F S+ + R + P + E C L P C + ++ Y+ C F GC G NNF S C +C R+ C P + C + +N + +CE+ YGGC + N F + +C++ C
Sbjct: 49 CAGYFPKFTFKYDTMSCEPYIYGGCGATANLYDTLQECMSSCVHGMRFCALLQILLCFNVVTARPGNKCSQPKETGPCRMILIAYYYNANTDRCESFYFGGCRGNDNNFKSLADCQKECHSSSYNPSSSELNPSVKS--EHCKSDPIAGQMFCAGYFDK---------FTFNYDTLTCEPYVYGGCGATANLYDTLQECMSSCVHGSAPTGGAPMESRQGKEAIKLPHWKEEEDICDLPPVHRGPITCEAYIEKWTYDKKSGNSCVHGQVGASVTEKTIPVSFFRSAPTGGAPMESRQDKDAIIFPQWKEEEDICDLPPVHKGPIMCAAYIEKWTYDKKRGKCVQFIHGGCFGTKNNFASKADCENSCPKTPSRSTKPPQFIRPDFCSLPAVDNEGGIQCLALSYGYTFNGAKGSCEQIVYGGCGATANYFSSLQKCKSAC 478 HSP 6 Score: 73.9442 bits (180), Expect = 1.020e-13 Identity = 42/132 (31.82%), Postives = 64/132 (48.48%), Query Frame = 0
Query: 501 EGCLLRPERGS--CHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC-HDAEVRADGSILTNASI--------CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
E C P G C G P+F ++ +C + + GC N + + Q C+++C H A IL ++ C QPK GPCR ++YN + + CE F +GGC+G+ NNF + A C+ C
Sbjct: 36 EHCKSEPVTGEIFCAGYFPKFTFKYDTMSCEPYIYGGCGATANLYDTLQECMSSCVHGMRFCALLQILLCFNVVTARPGNKCSQPKETGPCRMILIAYYYNANTDRCESFYFGGCRGNDNNFKSLADCQKEC 167 HSP 7 Score: 65.855 bits (159), Expect = 2.987e-11 Identity = 51/175 (29.14%), Postives = 74/175 (42.29%), Query Frame = 0
Query: 463 GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRP--------ERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASI----CEQPKLKGP--CRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
GC T N + T EC +C+ ++ + RP E G C L +YY + C SF F GC GN NNF+S C CH + S L N S+ C+ + G C +F +N TCE + YGGC + N + T +C ++C++
Sbjct: 72 GCGAT---ANLYDTLQECMSSCVHGMRFCALLQILLCFNVVTARPGNKCSQPKETGPCRMILIAYYYNANTDRCESFYFGGCRGNDNNFKSLADCQKECHSSSYNPSSSEL-NPSVKSEHCKSDPIAGQMFCAGYFDKFTFNYDTLTCEPYVYGGCGATANLYDTLQECMSSCVH 242 HSP 8 Score: 53.9138 bits (128), Expect = 1.449e-7 Identity = 25/62 (40.32%), Postives = 37/62 (59.68%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPC 296
C L G C ++ +N +T++CE F + GC+GNGN F S+EEC TC +PE++ C
Sbjct: 492 CTLPTEAGKCRARKPKYTFNQETQRCEKFFYTGCEGNGNGFDSVEECFDTCGGAEPEQNAFC 553
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000012691 (supercontig:LSalAtl2s:LSalAtl2s961:84758:119265:1 gene:EMLSAG00000012691 transcript:EMLSAT00000012691 description:"maker-LSalAtl2s961-snap-gene-1.23") HSP 1 Score: 127.872 bits (320), Expect = 1.752e-30 Identity = 98/335 (29.25%), Postives = 143/335 (42.69%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP-------EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKP--------ITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSD-RSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
C L G C + R++YN ++C+ FV+GGCKGNGNNF + +C C E + D C +G C G+ +F+FD ++ C F Y GCGGN NNF++ EC + C + VP + LKP ++ G+C + F++ + C +I+ GC G+DN F + E C C P+ V + + R E S SS L D + +S V C L + G C R+YY+P +TC F +SGC GN NNF+S + C+ TC
Sbjct: 1046 CTLPKNSGGCSNKIFRYYYNAIEQRCKLFVYGGCKGNGNNFLTERQCLLRCGEEGSLSQTEITTEVDTCSQKRDEGICPGNVPRFYFDKKAGRCLLFSYGGCGGNSNNFENQDECIARCGGPKGTII---------VPSSLTNGSLKPRKLICSLQLSRGNCXATLRRFYFDEKQG-CKLFIFGGCQGNDNNFETMEDCIRNCG------------------GPNEHGVSEFINQLR----------------EQSLSSSSTNLALPDLKENSANV--------CSLPKDFGHCMTVTSRYYYDPKTDTCKKFRYSGCGGNTNNFRSGERCIETC 1328 HSP 2 Score: 101.293 bits (251), Expect = 3.415e-22 Identity = 87/316 (27.53%), Postives = 133/316 (42.09%), Query Frame = 0
Query: 372 CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPRCRIEHTFEPCLPS------PSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSL--------EVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC------------HDAEVRADGSILTN----------ASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN-REVDAFIQFLAEESETFHEESTSS 647
C P SG C + Y +N E +C ++Y GC G+ N FL+ C RC E + I + C P + + ++ C++ GC N F EC C + + ++P + C L+ RG+C +L RFY++ + C F F GC GN NNF++ + C+ C + ++ S TN A++C PK G C R++Y+P +TC++FRY GC G+ NNF + +C TC E D I L T + +TSS
Sbjct: 1046 CTLPKNSGGCSNKIFRYYYNAIEQRCKLFVYGGCKGNGNNFLTERQCLLRCGEEGSLSQTEITTEVDTCSQKRDEGICPGNVPRFYFDKKAGRCLLFSYGGCGGN---SNNFENQDECIARCGGPKGTIIVPSSLTNGSLKPRKLI--CSLQLSRGNCXATLRRFYFDE-KQGCKLFIFGGCQGNDNNFETMEDCIRNCGGPNEHGVSEFINQLREQSLSSSSTNLALPDLKENSANVCSLPKDFGHCMTVTSRYYYDPKTDTCKKFRYSGCGGNTNNFRSGERCIETCGGLLEKDNRISALTLSPFTIIKATTSS 1355 HSP 3 Score: 62.3882 bits (150), Expect = 3.389e-10 Identity = 84/338 (24.85%), Postives = 124/338 (36.69%), Query Frame = 0
Query: 21 FCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMC-MNGNTTEEEILRQEPRRSLKNLTLDFDQG---------FEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGP----LRPQGFSKSH---PKLMHPIQVDRNEAITITLSL--------KLLRIESWPGRRNNSFIVMNDLV----------VLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPC----ISP--LLKGSCSGSGEKFHFDFQ 317
C L G C RY++N C+ F + GC N NNF ++ C C G+ ++ EI + S K D+G + D + SY N + D C GP + P + KL+ +Q+ R KL G NN F M D + V + LR ++++ ST LP E + + C L G C R++Y+ KT+ C+ F + GC GN NNF+S E C TC EKD+ +SP ++K + S + H F
Sbjct: 1045 ICTLPKNSGGCSNKIFRYYYNAIEQRCKLFVYGGCKGNGNNFLTERQCLLRCGEEGSLSQTEITTEVDTCSQKR-----DEGICPGNVPRFYFDKKAGRCLLFSYGGCGGNSNNFENQDECIARCGGPKGTIIVPSSLTNGSLKPRKLICSLQLSRGNCXATLRRFYFDEKQGCKLFIFGGCQGNDNN-FETMEDCIRNCGGPNEHGVSEFINQLREQSLSSSSTNLALPDL----------KENSANVCSLPKDFGHCMTVTSRYYYDPKTDTCKKFRYSGCGGNTNNFRSGERCIETCGGLL-EKDNRISALTLSPFTIIKATTSSTSNTLHKSFH 1365
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000000151 (supercontig:LSalAtl2s:LSalAtl2s1023:177971:188369:1 gene:EMLSAG00000000151 transcript:EMLSAT00000000151 description:"maker-LSalAtl2s1023-augustus-gene-1.11") HSP 1 Score: 122.865 bits (307), Expect = 3.918e-29 Identity = 118/488 (24.18%), Postives = 193/488 (39.55%), Query Frame = 0
Query: 17 VIPEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPG--RRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCS---EHKPEKDDPCISPLLKGSCSGSGE-KFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHI-----------FRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTC 484
++ C L + G C +Y+FN+ +CE+F++ GC N+N F C +C + +E+ P S K T + ED+D Y+ I N + N+ D C P+ +K P+ + +Q + W G + N+FI + + + S+I ++ TLP P+ C +FFYN K KC + CK N N + +++ +HK + C P G+C+ + KF+FD + + C KF YSGC GNGN FDS+ C +CK + +GR + + + C +G C+ Y N +C ++Y GC G+ N F S ++C+ C + C +E CL + Y + Q+ C+ + GC N FST +C++TC
Sbjct: 24 ILKSTCTLNKDPGTCAKNITQYYFNKDLKSCESFTWGGCEGNKNRFDTVLECNDVCQ---PSSKELSDNHPELSQKKKTQESSD--EDYDYYNLNFIQENKNEEVARNI--SDRDGSICGLPVEIGNCTKHSPRFFYNMQSGSCQFFI------------WKGCVKNENNFISIKKCLDKCHGVVKKESSITQVTSQCTLPPKNPS-----------------------CHELRSKFFYNPKKNKCVKRLH--CKDNPNGYXXIKDMLKAMQAKFKHKRKSIRRCFEPRDPGTCTKNFRLKFYFDSKQNKCLKFYYSGCNGNGNRFDSITNCVLSCKLKEQFSFQGVGRAVKYLNYLLDPKLLIIATPTYDTCSLQSETGPCEESVVQYFHNAKTGKCETFVYGGCYGNGNNFDSMKSCEEHCIQNSYNDACLVEKRRGYCLKK---KVAYHYDKNQQKCLSFIYGGCGGN---ANRFSTMDQCQKTC 461 HSP 2 Score: 121.324 bits (303), Expect = 1.336e-28 Identity = 107/461 (23.21%), Postives = 183/461 (39.70%), Query Frame = 0
Query: 230 LAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC-------SEHKPEKDDP--------------------------------------CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIA-PRCRIEHTFEPCLP---SPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLE------------VEPMFAL------EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
+ S C L G C + ++++N + CE+F +GGC+GN N F ++ EC C S++ PE C P+ G+C+ +F ++ QS +C F++ GC N NNF S+ +C C + + I +V S C P + C + + +N +N+CV ++ C + N + + + + R I FEP P + ++ + + +Q C+ GC+ GN F + + C +C S + ++P + + C L+ E G C S+ ++++ C +F + GC+GNGNNF S + C C C K +G C + + + Y+ + + C F YGGC G+ N F T QC+ TC
Sbjct: 24 ILKSTCTLNKDPGTCAKNITQYYFNKDLKSCESFTWGGCEGNKNRFDTVLECNDVCQPSSKELSDNHPELSQKKKTQESSDEDYDYYNLNFIQENKNEEVARNISDRDGSICGLPVEIGNCTKHSPRFFYNMQSGSCQFFIWKGCVKNENNFISIKKCLDKCHGVVKKESS-----ITQV----TSQCTLPPKNPSCHELRSKFFYNPKKNKCVKRLH--CKDNPNGYXXIKDMLKAMQAKFKHKRKSIRRCFEPRDPGTCTKNFRLKFYFDSKQNKCLKFYYSGCNGN---GNRFDSITNCVLSCKLKEQFSFQGVGRAVKYLNYLLDPKLLIIATPTYDTCSLQSETGPCEESVVQYFHNAKTGKCETFVYGGCYGNGNNFDSMKSCEEHCIQNSYN---------DACLVEKRRGYCLKKKVAYHYDKNQQKCLSFIYGGCGGNANRFSTMDQCQKTC 461 HSP 3 Score: 110.153 bits (274), Expect = 4.106e-25 Identity = 86/376 (22.87%), Postives = 150/376 (39.89%), Query Frame = 0
Query: 303 GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCK-------------------------DHMDPTMNSLGRHIFRVPKRSLSD-----CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPS------YTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLP-RFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAE---VRADGSILTNASICEQPKL----------------KGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
G+C+ + +++F+ +C F + GC GN N FD++ EC C+ D+ +N + + R++SD C P+ G+C + +N C +I+ GC ++N F+S + C ++C + I C P S + Y+ ++ CV + C + N + + + + H + ++ C + G+C + +FY++ C F +SGC+GNGN F S +C+ +C E + G + + PKL GPC ++F+N CE F YGGC G+ NNF + CE C+
Sbjct: 36 GTCAKNITQYYFNKDLKSCESFTWGGCEGNKNRFDTVLECNDVCQPSSKELSDNHPELSQKKKTQESSDEDYDYYNLNFIQENKNEEVARNISDRDGSICGLPVEIGNCTKHSPRFFYNMQSGSCQFFIWKGCVKNENNFISIKKCLDKCHGVVKKESSITQVTSQCTLPPKNPSCHELRSKFFYNPKKNKCVKRLHCKDNP---NGYXXIKDMLKAMQAKFKHKRK-----SIRRCFEPRDPGTCTKNFRLKFYFDSKQNKCLKFYYSGCNGNGNRFDSITNCVLSCKLKEQFSFQGVGRAVKYLNYLLDPKLLIIATPTYDTCSLQSETGPCEESVVQYFHNAKTGKCETFVYGGCYGNGNNFDSMKSCEEHCI 403 HSP 4 Score: 103.219 bits (256), Expect = 7.538e-23 Identity = 94/379 (24.80%), Postives = 158/379 (41.69%), Query Frame = 0
Query: 223 LSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCIS--------PLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLE-QGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEI------QIAPRC--RIEHTFEPCL---PSPSYTSVWVYSE----EQRMC-------------VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSI 564
++ + S+ GS C L + G C + RFFYN ++ C+ F++ GC N NNF S+++C C H K + I+ P SC KF ++ + + C K ++ C N N + + + M + F+ ++S+ C +P G C + + F+ +N+C+ + YSGC G+ N F S C C++ Q R + + +P L +P+Y + + SE E+ + V GC GN F + CE C+ + + + CL+ RG C ++Y+ + C SF + GC GN N F + C TC + + SI
Sbjct: 123 VARNISDRDGSICGLPVEIGNCTKHSPRFFYNMQSGSCQFFIWKGCVKNENNFISIKKCLDKC--HGVVKKESSITQVTSQCTLPPKNPSCHELRSKFFYNPKKNKCVKRLH--CKDNPNGYXXIKD------------MLKAMQAKFKHKRKSIRRCFEPRDPGTCTKNFRLKFYFDSKQNKCLKFYYSGCNGNGNRFDSITNCVLSCKLKEQFSFQGVGRAVKYLNYLLDPKLLIIATPTYDTCSLQSETGPCEESVVQYFHNAKTGKCETFVYGGCYGN---GNNFDSMKSCEEHCIQNSYN----------DACLVEKRRGYCLKKKVAYHYDKNQQKCLSFIYGGCGGNANRFSTMDQCQKTCTPIKALSSASI 472 HSP 5 Score: 95.9005 bits (237), Expect = 1.145e-20 Identity = 47/105 (44.76%), Postives = 64/105 (60.95%), Query Frame = 0
Query: 242 GPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
GPC ++ RF+YN + KCE+F FGGC NGN+FK LE C C+ ++ K CI P G C +KF+++ + C F+YSGCGGN N FDS +C+ C
Sbjct: 732 GPCQNFVKRFYYNANSTKCESFYFGGCFDNGNHFKELESCINHCAINEYPKS--CIEPQDVGECKEKIKKFYYEKDTGACHSFIYSGCGGNTNRFDSQRDCERIC 834 HSP 6 Score: 70.8626 bits (172), Expect = 7.423e-13 Identity = 45/143 (31.47%), Postives = 62/143 (43.36%), Query Frame = 0
Query: 505 LRPE-RGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTS 646
LRP+ G C + RFYY C SF F GC NGN+F+ + C+ C +I C +P+ G C+ +F+Y C F Y GC G+ N F ++ CE C V+ EE FH + S
Sbjct: 726 LRPKFIGPCQNFVKRFYYNANSTKCESFYFGGCFDNGNHFKELESCINHC---------AINEYPKSCIEPQDVGECKEKIKKFYYEKDTGACHSFIYSGCGGNTNRFDSQRDCERICSPYFVEK-----EEERVEFHNQKES 854 HSP 7 Score: 67.3958 bits (163), Expect = 8.052e-12 Identity = 39/133 (29.32%), Postives = 62/133 (46.62%), Query Frame = 0
Query: 290 PEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
PE+++ C+ P G C ++F+++ S C F + GC NGN+F L C + C I PK C++P G+C+ + + + + C +IYSGCGG+ N F S C+ C
Sbjct: 719 PEQEEICLRPKFIGPCQNFVKRFYYNANSTKCESFYFGGCFDNGNHFKELESCINHCA-------------INEYPK----SCIEPQDVGECKEKIKKFYYEKDTGACHSFIYSGCGGNTNRFDSQRDCERIC 834
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000008877 (supercontig:LSalAtl2s:LSalAtl2s554:325027:327698:-1 gene:EMLSAG00000008877 transcript:EMLSAT00000008877 description:"snap_masked-LSalAtl2s554-processed-gene-3.24") HSP 1 Score: 110.538 bits (275), Expect = 2.419e-25 Identity = 77/249 (30.92%), Postives = 107/249 (42.97%), Query Frame = 0
Query: 242 GPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-----CRIEHTFEPCLPS-PSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTC 484
G C + I++FY+ C F +GGC N+F + EEC C P C + GSC + +F++D++ +C FVY GC GNGN F S C C ++ D MN VP C KP+ G C + + ++ E +C + YSGCGG++N FLS C N C R CR + P + + W Y R C+ S FST EC C
Sbjct: 222 GSCSDFQIKWFYDATWGGCNKFWYGGCDPGENHFDTEEECIGICV--NPRGSQVCYLDMNTGSCDATYNEFYYDYKRGSCVPFVYGGCLGNGNRFTSKKSCNELCGNNND--MN--------VPV-----CSKPMAEGMCLGDFNRWYYDYEEGKCKNFSYSGCGGNNNRFLSEVECLNTCRKDTKERLSKTVCRSSYQITPSIKDGGTIFPAWTYDSLVRRCIPLYASSAEMEQDILFSTWEECSNIC 453 HSP 2 Score: 98.9821 bits (245), Expect = 1.289e-21 Identity = 62/197 (31.47%), Postives = 90/197 (45.69%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC-KDHMDPTMNSLGRHIFRV-PKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVP-YIYSGCGGSDNLFLSHEACKNRCEIQIAP 428
C L M G CD F+Y++K C FV+GGC GNGN F S + C C + C P+ +G C G ++++D++ C F YSGCGGN N F S EC +TC KD + ++ R +++ P + P + ++ +C+P Y S D LF + E C N C P
Sbjct: 274 CYLDMNTGSCDATYNEFYYDYKRGSCVPFVYGGCLGNGNRFTSKKSCNELCGNNNDMNVPVCSKPMAEGMCLGDFNRWYYDYEEGKCKNFSYSGCGGNNNRFLSEVECLNTCRKDTKERLSKTVCRSSYQITPSIKDGGTIFP-----------AWTYDSLVRRCIPLYASSAEMEQDILFSTWEECSNICPTTYPP 459 HSP 3 Score: 77.0258 bits (188), Expect = 9.139e-15 Identity = 62/237 (26.16%), Postives = 94/237 (39.66%), Query Frame = 0
Query: 389 GFNQTENQCVPYIYSGC--GGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
G +++ ++C Y C G + L L E C + ++ +C + C + W Y C GC + N F T EC C++ R + C L GSC + FYY+ +C F + GC GNGN F S + C C + N +C +P +G C D R++Y+ C+ F Y GC G+ N F + +C TC
Sbjct: 172 GASESCDECQNTKYGCCFDGNTTALGLHFEGCGSNLLEELPKKCSSKSRIGSC---SDFQIKWFYDATWGGCNKFWYGGCDPGE---NHFDTEEECIGICVNPRGSQV----------CYLDMNTGSCDATYNEFYYDYKRGSCVPFVYGGCLGNGNRFTSKKSCNELCGNNND-------MNVPVCSKPMAEGMCLGDFNRWYYDYEEGKCKNFSYSGCGGNNNRFLSEVECLNTC 385 HSP 4 Score: 52.373 bits (124), Expect = 3.720e-7 Identity = 33/121 (27.27%), Postives = 51/121 (42.15%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
C + GSC +++Y+ C+ F + GC N+F + + C+ C V GS +C G C F+Y+ +C F YGGC G+ N F ++ C C N
Sbjct: 215 CSSKSRIGSCSDFQIKWFYDATWGGCNKFWYGGCDPGENHFDTEEECIGIC----VNPRGS-----QVCYLDMNTGSCDATYNEFYYDYKRGSCVPFVYGGCLGNGNRFTSKKSCNELCGN 326
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000003009 (supercontig:LSalAtl2s:LSalAtl2s170:853996:854817:1 gene:EMLSAG00000003009 transcript:EMLSAT00000003009 description:"augustus_masked-LSalAtl2s170-processed-gene-8.0") HSP 1 Score: 93.2041 bits (230), Expect = 2.652e-21 Identity = 43/123 (34.96%), Postives = 67/123 (54.47%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C ++P+ G C LP+FYY+ C SF + GC GN N F + ++CL+ C E + ++C+ GPCR R+F++ + C EF YGGC+G+ NNF ++ CE C+ +
Sbjct: 137 CHMKPDPGPCRSLLPQFYYDFETGRCKSFYYGGCQGNANRFDNEENCLSICSSEEKKKGRE--NKGNVCKLKADPGPCRALFQRYFFDSEKKVCVEFGYGGCQGNENNFVKKSDCEDVCVKND 257 HSP 2 Score: 81.6481 bits (200), Expect = 2.619e-17 Identity = 41/129 (31.78%), Postives = 68/129 (52.71%), Query Frame = 0
Query: 223 LSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPE-----KDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
++ S+ S C + GPC L +F+Y+++T +C++F +GGC+GN N F + E C + CS + + K + C G C +++ FD + C +F Y GC GN NNF ++C+ C
Sbjct: 125 ITDMKSKEKPSQCHMKPDPGPCRSLLPQFYYDFETGRCKSFYYGGCQGNANRFDNEENCLSICSSEEKKKGRENKGNVCKLKADPGPCRALFQRYFFDSEKKVCVEFGYGGCQGNENNFVKKSDCEDVC 253 HSP 3 Score: 56.9954 bits (136), Expect = 3.937e-9 Identity = 36/120 (30.00%), Postives = 55/120 (45.83%), Query Frame = 0
Query: 303 GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
G C +F++DF++ C F Y GC GN N FD+ C S C +S + R K ++ C G C+ Y F+ + CV + Y GC G++N F+ C++ C
Sbjct: 144 GPCRSLLPQFYYDFETGRCKSFYYGGCQGNANRFDNEENCLSIC--------SSEEKKKGRENKGNV--CKLKADPGPCRALFQRYFFDSEKKVCVEFGYGGCQGNENNFVKKSDCEDVC 253 HSP 4 Score: 52.373 bits (124), Expect = 1.305e-7 Identity = 23/62 (37.10%), Postives = 35/62 (56.45%), Query Frame = 0
Query: 229 ELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP 290
E G+ C+L GPC R+F++ + + C F +GGC+GN NNF +CE C ++ P
Sbjct: 197 ENKGNVCKLKADPGPCRALFQRYFFDSEKKVCVEFGYGGCQGNENNFVKKSDCEDVCVKNDP 258
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Match: EMLSAG00000007269 (supercontig:LSalAtl2s:LSalAtl2s408:65936:69603:1 gene:EMLSAG00000007269 transcript:EMLSAT00000007269 description:"maker-LSalAtl2s408-snap-gene-0.18") HSP 1 Score: 97.8265 bits (242), Expect = 2.900e-21 Identity = 66/238 (27.73%), Postives = 105/238 (44.12%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLI--RFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKG--SCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCE-----IQIAPRCR----IEHTFEPCLPSPSYTSVWVYSEEQRMCV 459
C + G C Y I ++++N C F FGGC GNGN F+ CE+ C P C P + G SC+ ++FD + C +F+Y+GC GN N F + A C+ C M +L + + P C +P+ G C + ++ C ++Y GC G+ N F + E+C+N C+ ++I +CR + C S S + W + + C
Sbjct: 348 CHMTKDSGACRNYNITRQWYFNMAYGGCSRFWFGGCGGNGNRFEDRNSCESICI--NPSGTVKCYLPKVSGPPSCTSKQPHYYFDSNTKECREFIYNGCLGNANRFTTQASCEVEC-------MKALKKSGDQDP------CKQPVEPGPCNGYFPRWYYDFLRGTCSEFVYGGCKGNANQFETKESCQNSCKHKEMSLKIQKQCRKPRLVPTDGRDCHDSNSVVAKWYFDDNTHSCT 570 HSP 2 Score: 95.5153 bits (236), Expect = 1.574e-20 Identity = 76/266 (28.57%), Postives = 116/266 (43.61%), Query Frame = 0
Query: 235 CQLAMARGP--CDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSE--HKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGV---YGFNQTENQCVPYIYS-----GCG-------GSDNLFLSHEACKNRCEIQIAPRCRI---------EHTF---EPCLPSPSYTSVW------VYSEEQRMCVVTVG 463
C L GP C ++++ T++C F++ GC GN N F + CE C + K DPC P+ G C+G ++++DF TCS+FVY GC GN N F++ CQ++CK H + ++ ++ K+ L P DC V + F+ + C P+ S CG +D +F S E C + C AP I EHT +PS T +W + S + R+ + T G
Sbjct: 409 CYLPKVSGPPSCTSKQPHYYFDSNTKECREFIYNGCLGNANRFTTQASCEVECMKALKKSGDQDPCKQPVEPGPCNGYFPRWYYDFLRGTCSEFVYGGCKGNANQFETKESCQNSCK-HKEMSL--------KIQKQCRKPRLVPTDGRDCHDSNSVVAKWYFDDNTHSCTPFYLSKTDHLKCGSIMVEENANDTIFHSLEECMSICPNTYAPVISITSHVVIVKEEHTAVLQVQITSNPSPTIIWEFNGTLINSNDPRVSIDTPG 665 HSP 3 Score: 84.7297 bits (208), Expect = 3.720e-17 Identity = 52/163 (31.90%), Postives = 77/163 (47.24%), Query Frame = 0
Query: 471 GNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERG--SCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQ 631
GN F + CE C + P ++ C L G SC P +Y++ + C F ++GC GN N F + C C A ++ + C+QP GPC PR++Y+ TC EF YGGCKG+ N F T+ C+ +C ++E+ IQ
Sbjct: 387 GNRFEDRNSCESIC---------INPSGTVK-CYLPKVSGPPSCTSKQPHYYFDSNTKECREFIYNGCLGNANRFTTQASCEVECMKALKKSG-----DQDPCKQPVEPGPCNGYFPRWYYDFLRGTCSEFVYGGCKGNANQFETKESCQNSCKHKEMSLKIQ 534 HSP 4 Score: 73.1738 bits (178), Expect = 1.301e-13 Identity = 82/320 (25.62%), Postives = 128/320 (40.00%), Query Frame = 0
Query: 260 CENFVFGGCKGNGNNFK--SLEECETTCSEHKPEKDDPCISPLLKGSCSGSG--EKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSG--DCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSP--SYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERG-SCHGS---LPRFYYEPMDETCHSFTFSGC------------HGNGNNFQSFQHCLATC 553
CE FG C N + + EEC+ +KP D C G+C +++F+ CS+F + GCGGNGN F+ C+S C ++P+ C P SG C +Q Y F+ +C +IY+GC G+ N F + +C+ C + + +P P P Y W Y + C V GC K N+F T C+ +C + SL+++ L P G CH S + ++Y++ +C F S + N F S + C++ C
Sbjct: 317 CETSEFGCCPDGKNPARGENFEECQ-----NKPA--DICHMTKDSGACRNYNITRQWYFNMAYGGCSRFWFGGCGGNGNRFEDRNSCESIC---INPS--------------GTVKCYLPKVSGPPSCTSKQPHYYFDSNTKECREFIYNGCLGNANRFTTQASCEVECMKALKKSGDQDPCKQPVEPGPCNGYFPRWYYDFLRGTCSEFVYGGC---KGNANQFETKESCQNSC-KHKEMSLKIQKQCRKP--RLVPTDGRDCHDSNSVVAKWYFDDNTHSCTPFYLSKTDHLKCGSIMVEENANDTIFHSLEECMSIC 606 HSP 5 Score: 72.4034 bits (176), Expect = 2.236e-13 Identity = 52/166 (31.33%), Postives = 77/166 (46.39%), Query Frame = 0
Query: 234 PCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH----KPEKD--DPCISPLLKGSCSGSGE---KFHFDFQSHTCSKFVYS-----GCG-------GNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITS 378
PC+ + GPC+ Y R++Y++ C FV+GGCKGN N F++ E C+ +C K +K P + P C S K++FD +H+C+ F S CG N F SL EC S C + P + S+ H+ V + + ITS
Sbjct: 473 PCKQPVEPGPCNGYFPRWYYDFLRGTCSEFVYGGCKGNANQFETKESCQNSCKHKEMSLKIQKQCRKPRLVPTDGRDCHDSNSVVAKWYFDDNTHSCTPFYLSKTDHLKCGSIMVEENANDTIFHSLEECMSICPNTYAPVI-SITSHVVIVKEEHTAVLQVQITS 637
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|114152125|sp|Q868Z9.2|PPN_DROME (RecName: Full=Papilin; Flags: Precursor) HSP 1 Score: 185.267 bits (469), Expect = 2.630e-47 Identity = 129/419 (30.79%), Postives = 178/419 (42.48%), Query Frame = 0
Query: 229 ELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP------------EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRV---PKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRC---RIEHTFEPCL----PSPSYTSV--WVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
++A C++ G C Y+ ++Y+ + + C F +GGC GN N F + E C C + D C G CS K+HFD + C +F Y CGGNGN F++ +CQ C P P +++ C +P G C + +N+TE +C + Y GCGG+DN F + E C RC + I R +EH C P Y +V W Y+ + +C V GC N ++T EC+ C A C L P RG C R+Y++ CH F F+GC GN NNF S CL C E + S T S+C +P G C +FY+ C F Y GC G+ N F TR QCE C
Sbjct: 1843 QVAKDICEIPAEVGECANYVTSWYYDTQDQACRQFYYGGCGGNENRFPTEESCLARCDRKPEPTTTTPATRPQPSRQDVCDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRCLSQEPPAPTPPRAPAPTRQPDPAPTVAQCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARCSVNIDIRIGADPVEHDTSKCFLAFEPGNCYNNVTRWFYNSAEGLCDEFVYTGCGGN---ANNYATEEECQNECND------------AQTTCALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFCI-GEPVVEPSAPT-YSVCAEPPEAGECDNRTTAWFYDSENMACTAFTYTGCGGNGNRFETRDQCERQC 2244 HSP 2 Score: 167.933 bits (424), Expect = 1.243e-41 Identity = 113/400 (28.25%), Postives = 173/400 (43.25%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ--IAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCH-----DAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C L G C+ Y ++++++ C F +GGC GN N F+S EC+ TC ++ + C+ P G C+G +K++FD + C +F Y GC G N FDSL +CQ TC +L C +P+ SG C + ++ + C P+ Y GC G+ N + + AC C + RC + C + W +SE ++ CV GC K N F T CE C A + C + E G C + +YY+ D+ C F + GC GN N F + + CLA C A + +C++ G C ++ ++ I C +F YG C G+ N F T C+ CL++E
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQDYTGKH--VCLLPKSAGPCTGFTKKWYFDVDRNRCEEFQYGGCYGTNNRFDSLEQCQGTC-----------------AASENLPTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQPGVLKDRCALPKQTGDCS---EKLAKWHFSESEKRCVPFYYSGCGGNK---NNFPTLESCEDHCPR----------QVAKDICEIPAEVGECANYVTSWYYDTQDQACRQFYYGGCGGNENRFPTEESCLARCDRKPEPTTTTPATRPQPSRQDVCDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRCLSQE 1976 HSP 3 Score: 153.68 bits (387), Expect = 4.674e-37 Identity = 106/341 (31.09%), Postives = 161/341 (47.21%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLP---RFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTN 567
S C LA G C + R+FYN C+ FV+ GC GN NN+ + EEC+ C++ + C P ++G CS +++FD +S C +F ++GC GN NNF S ++C + C +G + + S C +P +G+C + ++ C + Y+GCGG+ N F + + C+ +C E + C T PC + + + ++ + C GC T GN FS EC+ CL+ R E A E CLL G C+G R+YY+ C SF ++GC GN NNF+SF+ C C + D I N
Sbjct: 2069 SKCFLAFEPGNCYNNVTRWFYNSAEGLCDEFVYTGCGGNANNYATEEECQNECNDAQTT----CALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFC----------IGEPVVEPSAPTYSVCAEPPEAGECDNRTTAWFYDSENMACTAFTYTGCGGNGNRFETRDQCERQCGEFKGVDVCNEPVTTGPCT---DWQTKYYFNTASQACEPFTYGGCDGT---GNRFSDLFECQTVCLAGR----EPRVGSAKEICLLPVATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQCRPEPNKQDNEIGQN 2385 HSP 4 Score: 152.14 bits (383), Expect = 1.443e-36 Identity = 107/413 (25.91%), Postives = 175/413 (42.37%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC----------------EIQIAPRCRIEHTFEPCLPS--PSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPM-FALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREV 626
C L G C L ++ ++ ++C F + GC GN NNF +LE CE C + D C P G C+ +++D Q C +F Y GCGGN N F + C + C D + + ++ + C + G+C + F++ C + Y CGG+ N F + C+ RC Q P + +P P + W Y+E + C GC N F+T EC C + + +P+ C L E G+C+ ++ R++Y + C F ++GC GN NN+ + + C C+DA+ + C P ++G C R++++ C EF + GC+G+RNNF +++ C C+ V
Sbjct: 1790 CALPKQTGDCSEKLAKWHFSESEKRCVPFYYSGCGGNKNNFPTLESCEDHCPRQVAK--DICEIPAEVGECANYVTSWYYDTQDQACRQFYYGGCGGNENRFPTEESCLARC-DRKPEPTTTTPATRPQPSRQDV--CDEEPAPGECSTWVLKWHFDRKIGACRQFYYGNCGGNGNRFETENDCQQRCLSQEPPAPTPPRAPAPTRQPDPAPTVAQCSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGND---NRFATEEECSARCSVNIDIRIGADPVEHDTSKCFLAFEPGNCYNNVTRWFYNSAEGLCDEFVYTGCGGNANNYATEEECQNECNDAQ-----------TTCALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFCIGEPV 2183 HSP 5 Score: 145.591 bits (366), Expect = 1.875e-34 Identity = 113/412 (27.43%), Postives = 163/412 (39.56%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCS------------EHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC--EIQIAPRCRIEHTFEPCLPSP------SYTSVWVYSEEQRMCVV--TVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRD---QPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C G CD + + + YN +C++F +GGC GN N F + EEC CS EH K C G+C + ++ ++ C +FVY+GCGGN NN+ + ECQ+ C D + + C P G C + F++ +C + ++GC G+ N F+S C N C E + P T+ C P + T+ W Y E C GC GN F T +CER C + + EP+ G C ++Y+ + C FT+ GC G GN F C C GS IC P G C + R++Y+ C F Y GC G++NNF + C C
Sbjct: 2001 CSQPADPGQCDKWALHWNYNETEGRCQSFYYGGCGGNDNRFATEEECSARCSVNIDIRIGADPVEHDTSK---CFLAFEPGNCYNNVTRWFYNSAEGLCDEFVYTGCGGNANNYATEEECQNECND-------------------AQTTCALPPVRGRCSDLSRRWYFDERSGECHEFEFTGCRGNRNNFVSQSDCLNFCIGEPVVEPS---APTYSVCAEPPEAGECDNRTTAWFYDSENMACTAFTYTGCGGN---GNRFETRDQCERQCGEFKGVDVCNEPVTT----------GPCTDWQTKYYFNTASQACEPFTYGGCDGTGNRFSDLFECQTVCLAGREPRVGSA---KEICLLPVATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQC 2371 HSP 6 Score: 106.301 bits (264), Expect = 3.475e-22 Identity = 77/270 (28.52%), Postives = 111/270 (41.11%), Query Frame = 0
Query: 372 CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESE 638
C P +G C Y F+ + C + Y GC G+DN F S CK+ C+ C + + PC +T W + ++ C GC T N F + +C+ TC + + L C E G C G+ R+YY+ + C FT+ GC GN NN+ + C C V D C PK G C ++ ++ S + C F Y GC G++NNFPT CE C + + AE E
Sbjct: 1612 CGLPKETGTCNNYSVKYYFDTSYGGCARFWYGGCDGNDNRFESEAECKDTCQDYTGKHVCLLPKSAGPCT---GFTKKWYFDVDRNRCEEFQYGGCYGTN---NRFDSLEQCQGTCAASEN----------LPTCEQPVESGPCAGNFERWYYDNETDICRPFTYGGCKGNKNNYPTEHACNYNCRQPGVLKD--------RCALPKQTGDCSEKLAKWHFSESEKRCVPFYYSGCGGNKNNFPTLESCEDHCPRQVAKDICEIPAEVGE 1857 HSP 7 Score: 88.9669 bits (219), Expect = 8.429e-17 Identity = 48/172 (27.91%), Postives = 82/172 (47.67%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEK----DDPCISPLLKGSCSGSG---EKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRH-----------------IFRVPKRSLSDCLKPITSGDCQ 382
C + GPC + ++++N ++ CE F +GGC G GN F L EC+T C + + + C+ P+ G C+G ++++D ++ C F+Y+GC GN NNF S C + C+ + N +G++ + RV RS +C + I C+
Sbjct: 2253 CNEPVTTGPCTDWQTKYYFNTASQACEPFTYGGCDGTGNRFSDLFECQTVCLAGREPRVGSAKEICLLPVATGRCNGPSVHERRWYYDDEAGNCVSFIYAGCSGNQNNFRSFEACTNQCRPEPNKQDNEIGQNPCDTFDAECQELRCPYGVRRVAARSQPECTQCICENPCE 2424
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|74960830|sp|O76840.1|PPN1_CAEEL (RecName: Full=Papilin; AltName: Full=Abnormal cell migration protein 6; Flags: Precursor) HSP 1 Score: 162.54 bits (410), Expect = 6.593e-40 Identity = 141/579 (24.35%), Postives = 228/579 (39.38%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSE------------------------------------HKPEKDDP--------------------CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD----------------------------------CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPRCRIEHTFEPC-LPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFA--LEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVAEIFIGIALSGLVILGVYFGFRLILEQVLVKEMQSHGSGLVFSDHFGQK----IELTDSFTDLFGV 713
CQL +GPC ++YN + +CE F +GGC GN N F ++EEC+ C + H +D P C + GSC +H+ S++C +F Y GC GN N F++ +C+++C ++ + S+ + + L+D C+ P G C + F+ ++QCV ++YSGC + N F S E C+ C + + C + C L P W + + C ++ GC GN FS+ ++CE C VE +++ + C L G C S+ +Y++ C FT+ GC GN N F S + C +C + +++ IC GPCR ++FY+P I++C F YGGC+G+ N F + C C + +V+ A ESE + +++S V I I + +G F R VL +G L F ++ +E+ D+ T GV
Sbjct: 1504 CQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARCPKDDQTTTTSQPEELPSLPLVQEDPQPRPAFSLKQSFAHSRRRDAPFARSVSARHHTPDSEEERVDCYAVPDPGSCGDYRLVWHYSATSNSCRQFYYGGCAGNTNRFETRDKCETSCVAKIEERVESVSEASKSLEEVRLTDPRMDSHFGYHDPEVDQIEEEAEYVIVDTGALPELCMLPEQRGSCYDNILRWRFDSEKSQCVTFMYSGCNPNANHFTSQETCERACGKWRNVAVCELPAEHGDCQLAIPR----WYHDPKTSQCQMMMWTGCGGN---GNAFSSKADCESLC--------RVETLWSNNTDFCTLERSAGPCTDSISMWYFDSTHLDCKPFTYGGCRGNQNRFVSKEQCQQSCRPGDTKSE-------DICTLRPEPGPCRLGLEKYFYDPVIQSCHMFHYGGCEGNANRFDSELDCFRRCSSVKVE------ASESERVGQLTSASTPV-----IYIVNKTAIFVGNTFRIRCNSYGVLPITWYKNGGLLQFGSRITEENDDTLEIVDALTADAGV 2049 HSP 2 Score: 119.013 bits (297), Expect = 3.367e-26 Identity = 112/478 (23.43%), Postives = 181/478 (37.87%), Query Frame = 0
Query: 216 STTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEK-----------DDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPS--PSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC----------------------------------HDAEV-----RADGSILTNAS---ICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESE 638
S +P S+S A C L + +G C ++Y T C F + GC GN N F S ++CE+ C + E + C G C+ K++++ TC++F Y GC G N FD+ +C++ C++H D C P G C + Y +N +QC + Y GC G+ N F + E C+ RC + + T P PS V + + + ++++R F+ S +S R H+ + E C P+ GSC ++Y +C F + GC GN N F++ C +C HD EV A+ I+ + +C P+ +G C + R+ ++ C F Y GC + N+F ++ CE C A + AE +
Sbjct: 1359 SASPVVVPSNSKQRDA---CHLNVDQGRCKGAFDSWYYEVATGSCVTFKYTGCGGNANRFASKDQCESLCVKPASEAASAGIDGAAGINSVCDEAKDTGPCTNFVTKWYYNKADGTCNRFHYGGCQGTNNRFDNEQQCKAACQNHKDA-------------------CQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARCP-------KDDQTTTTSQPEELPSLPLVQEDPQPRPAFSLKQSFAHSRRRDAPFARS-------VSARHHTPDSEEERV--DCYAVPDPGSCGDYRLVWHYSATSNSCRQFYYGGCAGNTNRFETRDKCETSCVAKIEERVESVSEASKSLEEVRLTDPRMDSHFGYHDPEVDQIEEEAEYVIVDTGALPELCMLPEQRGSCYDNILRWRFDSEKSQCVTFMYSGCNPNANHFTSQETCERACGKWRNVAVCELPAEHGD 1798 HSP 3 Score: 93.5893 bits (231), Expect = 3.079e-18 Identity = 99/443 (22.35%), Postives = 160/443 (36.12%), Query Frame = 0
Query: 293 DDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC----------------KDHMDPTMNSLGRHIFRVPKRSLSDCLKP------------ITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC--------------EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSI---LTNASICEQPKLKGPC-----------RRDQP---------------------------------RFFYNPSI--ETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHE 642
+D C S G C +++ ++ S C F Y GCGGN N F S EC+ C + P + +I P++S S + P + G C+ + + CV + Y+GCGG+ N F S + C++ C I C PC ++ + W Y++ C GC T N F +C+ C + + + C L +G C G +YY C +FT+ GC GN N F + + C A C + S L + + ++ P RRD P R ++ S +C +F YGGC G+ N F TR +CE +C+ + ++ ++ ++E S++ E
Sbjct: 1268 EDICRSRQDAGPCETYSDQWFYNAFSQECETFTYGGCGGNLNRFRSKDECEQRCFFVHGAQPSAARQEQAQPAAQPAQPAQPSNIVSPPQQSASPVVVPSNSKQRDACHLNVDQGRCKGAFDSWYYEVATGSCVTFKYTGCGGNANRFASKDQCESLCVKPASEAASAGIDGAAGINSVCDEAKDTGPCT---NFVTKWYYNKADGTCNRFHYGGCQGTN---NRFDNEQQCKAACQNHK------------DACQLPKVQGPCSGKHSYYYYNTASHQCETFTYGGCLGNTNRFATIEECQARCPKDDQTTTTSQPEELPSLPLVQEDPQPRPAFSLKQSFAHSRRRDAPFARSVSARHHTPDSEEERVDCYAVPDPGSCGDYRLVWHYSATSNSCRQFYYGGCAGNTNRFETRDKCETSCVAK-IEERVESVSEASKSLEE 1691 HSP 4 Score: 73.559 bits (179), Expect = 4.295e-12 Identity = 90/387 (23.26%), Postives = 146/387 (37.73%), Query Frame = 0
Query: 290 PEKDDPCISPLLKGSCSGSGEK--FHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGR-HIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAP---------RCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC---HDAE-----------------VRADGSILTNASICEQPKL----------------KGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDA 628
P K C G+ G+G K +H+D C++F Y GCGGN NNF S C++ C ++P GR ++ RV D L+P Y ++ ++ CV + + GC G+ N F S E C C+ + P + + LP+P + + S EQ ++ + S E C S R + G C +++Y + C +FT+ GC GN N F+S C C H A+ +I++ P + +G C+ ++Y + +C F+Y GC G+ N F ++ QCE+ C+ +A
Sbjct: 1083 PHKIAACNQTQESGTVCGAGYKLAWHYDTTEGRCNQFWYGGCGGNDNNFASQDMCETIC---VEPP--GKGRCYLPRVDGPLRCDQLQP-----------RYYYDHSKKHCVAFWWRGCLGNANNFNSFEECSMFCK-DVGPYDAPTTAAPPPPPQQNAQQYLPTPEVQQIEIQSAEQPQPQQPQQQQQQQQQQPQQPRQSM-EDICRS-------------------RQDAGPCETYSDQWFYNAFSQECETFTYGGCGGNLNRFRSKDECEQRCFFVHGAQPSAARQEQAQPAAQPAQPAQPSNIVSPPQQSASPVVVPSNSKQRDACHLNVDQGRCKGAFDSWYYEVATGSCVTFKYTGCGGNANRFASKDQCESLCVKPASEA 1432 HSP 5 Score: 73.1738 bits (178), Expect = 5.451e-12 Identity = 46/138 (33.33%), Postives = 64/138 (46.38%), Query Frame = 0
Query: 215 PSTTPASTLSSSTSELAGSPCQLAMARGP-CDL-YLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKG--SCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD 348
P+TTP + + + + C G C Y + + Y+ +C F +GGC GN NNF S + CET C E P C P + G C ++++D C F + GC GN NNF+S EC CKD
Sbjct: 1069 PTTTPPFLMGGTVAPHKIAACNQTQESGTVCGAGYKLAWHYDTTEGRCNQFWYGGCGGNDNNFASQDMCETICVE--PPGKGRCYLPRVDGPLRCDQLQPRYYYDHSKKHCVAFWWRGCLGNANNFNSFEECSMFCKD 1204 HSP 6 Score: 65.4698 bits (158), Expect = 1.565e-9 Identity = 78/304 (25.66%), Postives = 114/304 (37.50%), Query Frame = 0
Query: 4 DQIPEGGNGSVEHVI------PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIES---------WPGRRNNSFIVMNDLVVL-QTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPE 291
DQI E E+VI PE C+L +RG C+ R+ F+ C TF +SGC PN N+F ++ CER C L E + L + + D Q++ + G N +S C R + ++ ++R+ A T S+ + +S + G R N N V Q QS RP ++ T P P C L L ++FY+ + C F +GGC+GN N F S +C CS K E
Sbjct: 1711 DQIEE----EAEYVIVDTGALPELCMLPEQRGSCYDNILRWRFDSEKSQCVTFMYSGCNPNANHFTSQETCERACGKWRNVAVCELPAE----HGDCQLAIPRWYHDPKTSQCQMMMWTGCGGNG-NAFS---SKADCESLCRVETLWSNNTDF---CTLERS-AGPCTDSISMWYFDSTHLDCKPFTYGGCRGN----QNRFVSKEQCQQSCRPGDTKSEDICTLRPEPGP------------------------CRLGLEKYFYDPVIQSCHMFHYGGCEGNANRFDSELDCFRRCSSVKVE 1970
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|125934|sp|P19761.2|TFPI1_RABIT (RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor) HSP 1 Score: 138.658 bits (348), Expect = 3.151e-35 Identity = 80/221 (36.20%), Postives = 116/221 (52.49%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPE----------KDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCK---------DH---MDPTMNSLGRHIFRVPKRSL----SDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR 429
C + + GPC Y+ RFF+N T +CE F++GGC+GN N F+SLEEC+ C+ P+ K D C G C G ++ ++ QS C +F Y GC GN NNF+SL EC++TC+ DH ++ N+L + P+R S CL P G CQ + + +N +C P+ YSGCGG++N F S +AC C+ P+
Sbjct: 50 CAMKVDDGPCRAYIKRFFFNILTHQCEEFIYGGCEGNENRFESLEECKEKCARDYPKMTTKLTFQKGKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNLNNFESLEECKNTCENPTSDFQVDDHRTQLNTVNNTLINQPTKAPRRWAFHGPSWCLPPADRGLCQANEIRFFYNAIIGKCRPFKYSGCGGNENNFTSKKACITACKKGFIPK 270 HSP 2 Score: 83.5741 bits (205), Expect = 2.268e-16 Identity = 52/177 (29.38%), Postives = 76/177 (42.94%), Query Frame = 0
Query: 472 NEFSTSSECERTCLSDRSHSLEVEPMFAL---EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC-----------HDAEVRADGSILTN-------------ASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
N F + EC+ C D + + F + C L + G C G + R++Y + C F + GC GN NNF+S + C TC H ++ + L N S C P +G C+ ++ RFFYN I C F+Y GC G+ NNF ++ C C
Sbjct: 88 NRFESLEECKEKCARDYP-KMTTKLTFQKGKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNLNNFESLEECKNTCENPTSDFQVDDHRTQLNTVNNTLINQPTKAPRRWAFHGPSWCLPPADRGLCQANEIRFFYNAIIGKCRPFKYSGCGGNENNFTSKKACITAC 263 HSP 3 Score: 77.0258 bits (188), Expect = 3.821e-14 Identity = 40/128 (31.25%), Postives = 60/128 (46.88%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSIL---TNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
C ++ + G C + RF++ + C F + GC GN N F+S + C C + + C + G CR R+FYN + CE F+YGGC G+ NNF + +C+ TC N D
Sbjct: 50 CAMKVDDGPCRAYIKRFFFNILTHQCEEFIYGGCEGNENRFESLEECKEKCARDYPKMTTKLTFQKGKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNLNNFESLEECKNTCENPTSD 177 HSP 4 Score: 74.3294 bits (181), Expect = 3.514e-13 Identity = 62/229 (27.07%), Postives = 96/229 (41.92%), Query Frame = 0
Query: 366 KRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPS-----------PSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTC---------------LSDRSHSLEVEPM-----FALEG---CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEV 558
K + S C + G C+ + FN +QC +IY GC G++N F S E CK +C + P+ + TF+ P Y + + Y+ + + C GC N F + EC+ TC L+ +++L +P +A G CL +RG C + RF+Y + C F +SGC GN NNF S + C+ C +
Sbjct: 44 KPTHSFCAMKVDDGPCRAYIKRFFFNILTHQCEEFIYGGCEGNENRFESLEECKEKC-ARDYPKMTTKLTFQKGKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNL---NNFESLEECKNTCENPTSDFQVDDHRTQLNTVNNTLINQPTKAPRRWAFHGPSWCLPPADRGLCQANEIRFFYNAIIGKCRPFKYSGCGGNENNFTSKKACITACKKGFI 268 HSP 5 Score: 56.6102 bits (135), Expect = 2.051e-7 Identity = 28/57 (49.12%), Postives = 33/57 (57.89%), Query Frame = 0
Query: 19 PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNG 75
P +CL +RG C E R+F+N G C F +SGCG NENNF KKAC C G
Sbjct: 210 PSWCLPPADRGLCQANEIRFFYNAIIGKCRPFKYSGCGGNENNFTSKKACITACKKG 266
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|125932|sp|P10646.1|TFPI1_HUMAN (RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor) HSP 1 Score: 129.413 bits (324), Expect = 6.622e-32 Identity = 76/227 (33.48%), Postives = 111/227 (48.90%), Query Frame = 0
Query: 229 ELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH----------KPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD------------HMDPTMNSLGRHIFRVPK----RSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR 429
+L S C GPC + RFF+N T +CE F++GGC+GN N F+SLEEC+ C+ + EK D C G C G ++ ++ Q+ C +F Y GC GN NNF++L EC++ C+D ++ NSL +VP S CL P G C+ + + +N +C P+ YSGCGG++N F S + C C+ R
Sbjct: 48 KLMHSFCAFKADDGPCKAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMCTRDNANRIIKTTLQQEKPDFCFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDGPNGFQVDNYGTQLNAVNNSLTPQSTKVPSLFEFHGPSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRACKKGFIQR 274 HSP 2 Score: 90.1225 bits (222), Expect = 1.619e-18 Identity = 73/261 (27.97%), Postives = 102/261 (39.08%), Query Frame = 0
Query: 303 GSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD-CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG---CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVR 559
G C ++F F+ + C +F+Y GC GN N F+SL EC+ C T ++ R I ++ D C G C+ Y +N QC + Y GC G+ N F + E CKN CE P+ V Y + + N + S +V +F G CL +RG C + RFYY + C F +SGC GN NNF S Q CL C ++
Sbjct: 61 GPCKAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMC------TRDNANRIIKTTLQQEKPDFCFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICE-----------------DGPNGFQVDNYG------------TQLNAVNNSLT-------------PQSTKVPSLFEFHGPSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRACKKGFIQ 273 HSP 3 Score: 88.5817 bits (218), Expect = 5.458e-18 Identity = 55/194 (28.35%), Postives = 87/194 (44.85%), Query Frame = 0
Query: 472 NEFSTSSECERTCLSDRSHSL------EVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA-----------EVRADGSILT-------------NASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAE 635
N F + EC++ C D ++ + + +P F C L + G C G + R++Y + C F + GC GN NNF++ + C C D ++ A + LT S C P +G CR ++ RF+YN I C F+Y GC G+ NNF ++ +C C FIQ +++
Sbjct: 92 NRFESLEECKKMCTRDNANRIIKTTLQQEKPDF----CFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDGPNGFQVDNYGTQLNAVNNSLTPQSTKVPSLFEFHGPSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRAC----KKGFIQRISK 277 HSP 4 Score: 56.225 bits (134), Expect = 2.864e-7 Identity = 26/57 (45.61%), Postives = 33/57 (57.89%), Query Frame = 0
Query: 19 PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNG 75
P +CL +RG C E R+++N G C F +SGCG NENNF K+ C R C G
Sbjct: 214 PSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRACKKG 270
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|6094466|sp|Q28864.1|TFPI1_MACMU (RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor) HSP 1 Score: 127.102 bits (318), Expect = 3.872e-31 Identity = 80/251 (31.87%), Postives = 118/251 (47.01%), Query Frame = 0
Query: 199 SLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEH----------KPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD------------HMDPTMNSLGRHIFRVPK----RSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCE 423
+L P+ + DS + + L +L S C GPC + RFF+N T +CE F++GGC GN N F+S+EEC+ C+ + EK D C G C G ++ ++ QS C +F Y GC GN NNF++L EC++TC+D ++ NS +VP S CL P G C+ + + +N +C P+ YSGCGG++N F S C C+
Sbjct: 20 NLAPAPLNADSEEDEEYTIITDTELPPL--KLMHSFCAFKPDDGPCKAIMKRFFFNIFTRQCEEFIYGGCGGNQNRFESMEECKKVCTRDNVHRIIQTALQQEKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNMNNFETLEECKNTCEDGLNGFQVDNYGTQLNAVNNSQTPQSTKVPSFFEFHGPSWCLAPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKRECLRACK 268 HSP 2 Score: 92.4337 bits (228), Expect = 2.762e-19 Identity = 56/191 (29.32%), Postives = 85/191 (44.50%), Query Frame = 0
Query: 472 NEFSTSSECERTCLSDRSHSLEVEPMFALEG---CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA----EVRADGSILTNA--------------------SICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAE 635
N F + EC++ C D H + ++ E C L + G C G + R++Y + C F + GC GN NNF++ + C TC D +V G+ L S C P +G CR ++ RF+YN I C F+Y GC G+ NNF ++ +C C FIQ +++
Sbjct: 92 NRFESMEECKKVCTRDNVHRI-IQTALQQEKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNMNNFETLEECKNTCEDGLNGFQVDNYGTQLNAVNNSQTPQSTKVPSFFEFHGPSWCLAPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKRECLRAC----KKGFIQRISK 277 HSP 3 Score: 83.1889 bits (204), Expect = 3.618e-16 Identity = 44/125 (35.20%), Postives = 60/125 (48.00%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKL------KGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C +P+ G C + RF++ C F + GC GN N F+S + C C V I+ A E+P G CR R+FYN + CE F+YGGC G+ NNF T +C+ TC
Sbjct: 54 CAFKPDDGPCKAIMKRFFFNIFTRQCEEFIYGGCGGNQNRFESMEECKKVCTRDNVH---RIIQTALQQEKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNMNNFETLEECKNTC 175 HSP 4 Score: 56.6102 bits (135), Expect = 2.266e-7 Identity = 26/57 (45.61%), Postives = 33/57 (57.89%), Query Frame = 0
Query: 19 PEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNG 75
P +CL +RG C E R+++N G C F +SGCG NENNF K+ C R C G
Sbjct: 214 PSWCLAPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKRECLRACKKG 270
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|401174|sp|Q02445.1|TFPI1_RAT (RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor) HSP 1 Score: 117.087 bits (292), Expect = 1.255e-27 Identity = 66/220 (30.00%), Postives = 105/220 (47.73%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC----------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTM------------------NSLGRHIFRVPKRSLSD----CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
C + GPC + +++N + +CE F++GGC+GN N F +LEEC TC + EK D C G C G ++ ++ QS C +F Y GC GN NNF++L EC++TC+D ++ N + + P + D CL+P SG C+ + + +N +C + Y+GCGG++N F + + C C
Sbjct: 53 CAMKAEDGPCKAMIRSYYFNMNSHQCEEFIYGGCRGNKNRFDTLEECRKTCIPGYKKTTIKTTSGAEKPDFCFLEEDPGICRGFMTRYFYNNQSKQCEQFKYGGCLGNSNNFETLEECRNTCEDPVNEVQKGDYVTNQITVTDRTTVNNVVIPQATKAPSQWDYDGPSWCLEPADSGLCKASEKRFYYNPAIGKCRQFNYTGCGGNNNNFTTKQDCNRAC 272 HSP 2 Score: 93.9745 bits (232), Expect = 7.901e-20 Identity = 60/195 (30.77%), Postives = 86/195 (44.10%), Query Frame = 0
Query: 463 GCSNTKRIGNEFSTSSECERTCL------SDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA--EVR-----------ADGSILTNASI-----------------CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
GC K N F T EC +TC+ + ++ S +P F C L + G C G + R++Y + C F + GC GN NNF++ + C TC D EV+ D + + N I C +P G C+ + RF+YNP+I C +F Y GC G+ NNF T+ C C
Sbjct: 85 GCRGNK---NRFDTLEECRKTCIPGYKKTTIKTTSGAEKPDF----CFLEEDPGICRGFMTRYFYNNQSKQCEQFKYGGCLGNSNNFETLEECRNTCEDPVNEVQKGDYVTNQITVTDRTTVNNVVIPQATKAPSQWDYDGPSWCLEPADSGLCKASEKRFYYNPAIGKCRQFNYTGCGGNNNNFTTKQDCNRAC 272 HSP 3 Score: 82.0333 bits (201), Expect = 7.825e-16 Identity = 49/168 (29.17%), Postives = 75/168 (44.64%), Query Frame = 0
Query: 493 EVEPMFALEG-CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKL------KGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVVAE 653
E+ PM L C ++ E G C + +Y+ C F + GC GN N F + + C TC + + + S E+P G CR R+FYN + CE+F+YGGC G+ NNF T +C TC + + T + +T + VV+ +
Sbjct: 42 ELRPMKPLHTFCAMKAEDGPCKAMIRSYYFNMNSHQCEEFIYGGCRGNKNRFDTLEECRKTCIPGYKK---TTIKTTSGAEKPDFCFLEEDPGICRGFMTRYFYNNQSKQCEQFKYGGCLGNSNNFETLEECRNTCEDPVNEVQKGDYVTNQITVTDRTTVNNVVIPQ 206 HSP 4 Score: 81.6481 bits (200), Expect = 1.257e-15 Identity = 47/163 (28.83%), Postives = 75/163 (46.01%), Query Frame = 0
Query: 221 STLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSE-----------------------------------HKPEKDDP--CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
+T+ +++ C L G C ++ R+FYN ++++CE F +GGC GN NNF++LEEC TC + + + D P C+ P G C S ++F+++ C +F Y+GCGGN NNF + +C C
Sbjct: 110 TTIKTTSGAEKPDFCFLEEDPGICRGFMTRYFYNNQSKQCEQFKYGGCLGNSNNFETLEECRNTCEDPVNEVQKGDYVTNQITVTDRTTVNNVVIPQATKAPSQWDYDGPSWCLEPADSGLCKASEKRFYYNPAIGKCRQFNYTGCGGNNNNFTTKQDCNRAC 272
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|13124581|sp|O54819.1|TFPI1_MOUSE (RecName: Full=Tissue factor pathway inhibitor; Short=TFPI; AltName: Full=Extrinsic pathway inhibitor; Short=EPI; AltName: Full=Lipoprotein-associated coagulation inhibitor; Short=LACI; Flags: Precursor) HSP 1 Score: 115.931 bits (289), Expect = 2.999e-27 Identity = 71/258 (27.52%), Postives = 115/258 (44.57%), Query Frame = 0
Query: 207 TDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC----------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD--HMDPTMNSLGRHIFRVPKRSLSD--------------------------CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQI 426
D T + L S P T C + GPC + +F+N T +CE F++GGC+GN N F +LEEC+ TC + E+ D C G C G +++ ++ Q+ C +FVY GC GN NNF++L EC+ C++ H +N + + +++D CL+P SG C+ + + +N +C + Y+GCGG++N F + C C+ +
Sbjct: 33 ADDTDSELGSMKPLHTF-----------CAMKADDGPCKAMIRSYFFNMYTHQCEEFIYGGCEGNENRFDTLEECKKTCIPGYEKTAVKAASGAERPDFCFLEEDPGLCRGYMKRYLYNNQTKQCERFVYGGCLGNRNNFETLDECKKICENPVHSPSPVNEVQMSDYVTDGNTVTDRSTVNNIVVPQSPKVPRRRDYRGRPWCLQPADSGLCKASERRFYYNSATGKCHRFNYTGCGGNNNNFTTRRRCLRSCKTGL 279 HSP 2 Score: 84.3445 bits (207), Expect = 1.819e-16 Identity = 54/192 (28.12%), Postives = 80/192 (41.67%), Query Frame = 0
Query: 472 NEFSTSSECERTCL------SDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHD-------------AEVRADGSILTNASI-----------------------CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
N F T EC++TC+ + ++ S P F C L + G C G + R+ Y + C F + GC GN NNF++ C C + ++ DG+ +T+ S C QP G C+ + RF+YN + C F Y GC G+ NNF TR +C +C
Sbjct: 88 NRFDTLEECKKTCIPGYEKTAVKAASGAERPDF----CFLEEDPGLCRGYMKRYLYNNQTKQCERFVYGGCLGNRNNFETLDECKKICENPVHSPSPVNEVQMSDYVTDGNTVTDRSTVNNIVVPQSPKVPRRRDYRGRPWCLQPADSGLCKASERRFYYNSATGKCHRFNYTGCGGNNNNFTTRRRCLRSC 275 HSP 3 Score: 76.2554 bits (186), Expect = 8.552e-14 Identity = 40/127 (31.50%), Postives = 59/127 (46.46%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKL------KGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLN 623
C ++ + G C + +++ C F + GC GN N F + + C TC + + + AS E+P G CR R+ YN + CE F YGGC G+RNNF T +C+ C N
Sbjct: 50 CAMKADDGPCKAMIRSYFFNMYTHQCEEFIYGGCEGNENRFDTLEECKKTCIPG---YEKTAVKAASGAERPDFCFLEEDPGLCRGYMKRYLYNNQTKQCERFVYGGCLGNRNNFETLDECKKICEN 173
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|124015206|sp|P84875.1|PCPI_SABMA (RecName: Full=Carboxypeptidase inhibitor SmCI) HSP 1 Score: 103.99 bits (258), Expect = 1.443e-24 Identity = 60/190 (31.58%), Postives = 90/190 (47.37%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
S C L RG C Y+ ++F+N TE CE FV+GGC+GN N F++ ++C C + P K PC S + + ++ C F+Y GC GN N F + ECQ C + P C +P +G C+ Y ++ + +C +IY GC G+ N F + E+C+N C
Sbjct: 2 SVCDLPADRGQCTAYIPQWFFNKTTEDCEKFVYGGCQGNANRFETKDDCIANCGCNLPSKVGPCRV---------SARMWFHNPETEKCEVFIYGGCHGNANRFATETECQEVCDRYQKPGF-----------------CYQPSETGPCKGSFPRYYYDYEDGECKEFIYGGCEGNANNFETKESCENAC 165 HSP 2 Score: 93.2041 bits (230), Expect = 8.707e-21 Identity = 45/127 (35.43%), Postives = 70/127 (55.12%), Query Frame = 0
Query: 220 ASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
A+ + +A C L GPC + +F+N +TEKCE F++GGC GN N F + EC+ C + +K C P G C GS ++++D++ C +F+Y GC GN NNF++ C++ C
Sbjct: 41 ANRFETKDDCIANCGCNLPSKVGPCRVSARMWFHNPETEKCEVFIYGGCHGNANRFATETECQEVCDRY--QKPGFCYQPSETGPCKGSFPRYYYDYEDGECKEFIYGGCEGNANNFETKESCENAC 165 HSP 3 Score: 83.1889 bits (204), Expect = 2.222e-17 Identity = 59/187 (31.55%), Postives = 82/187 (43.85%), Query Frame = 0
Query: 369 LSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
+S C P G C + FN+T C ++Y GC G+ N F + + C C C + PC S +W ++ E C V + GC N F+T +EC+ C DR +P F C E G C GS PR+YY+ D C F + GC GN NNF++ + C C
Sbjct: 1 ISVCDLPADRGQCTAYIPQWFFNKTTEDCEKFVYGGCQGNANRFETKDDCIANC------GCNLPSKVGPCRVS---ARMWFHNPETEKCEVFIYGGCHGN---ANRFATETECQEVC--DRYQ----KPGF----CYQPSETGPCKGSFPRYYYDYEDGECKEFIYGGCEGNANNFETKESCENAC 165 HSP 4 Score: 82.8037 bits (203), Expect = 3.704e-17 Identity = 54/180 (30.00%), Postives = 74/180 (41.11%), Query Frame = 0
Query: 444 SYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
+Y W +++ C V GC N F T +C C GC L + G C S +++ P E C F + GCHGN N F + C C + C QP GPC+ PR++Y+ C+EF YGGC+G+ NNF T+ CE C
Sbjct: 15 AYIPQWFFNKTTEDCEKFVYGGCQGN---ANRFETKDDCIANC-----------------GCNLPSKVGPCRVSARMWFHNPETEKCEVFIYGGCHGNANRFATETECQEVCDRYQ---------KPGFCYQPSETGPCKGSFPRYYYDYEDGECKEFIYGGCEGNANNFETKESCENAC 165 HSP 5 Score: 82.0333 bits (201), Expect = 6.734e-17 Identity = 42/127 (33.07%), Postives = 58/127 (45.67%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAF 629
C L +RG C +P++++ E C F + GC GN N F++ C+A C C P GPCR +F+NP E CE F YGGC G+ N F T +C+ C + F
Sbjct: 4 CDLPADRGQCTAYIPQWFFNKTTEDCEKFVYGGCQGNANRFETKDDCIANCG----------------CNLPSKVGPCRVSARMWFHNPETEKCEVFIYGGCHGNANRFATETECQEVCDRYQKPGF 114
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|1351226|sp|P48307.1|TFPI2_HUMAN (RecName: Full=Tissue factor pathway inhibitor 2; Short=TFPI-2; AltName: Full=Placental protein 5; Short=PP5; Flags: Precursor) HSP 1 Score: 105.916 bits (263), Expect = 1.914e-24 Identity = 63/186 (33.87%), Postives = 84/186 (45.16%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNG--NNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEAC 418
C L + GPC L+R++Y+ T+ C F++GGC+GN NNF + E C+ C + + + C GS EK+ F+ S TC KF GC N N F A C C PK+ S C P G C Y FN C + Y+GCGG+DN F+S E C
Sbjct: 36 CLLPLDYGPCRALLLRYYYDRYTQSCRQFLYGGCEGNANNFYTWEACDDACWRIEKVPKVCRLQVSVDDQCEGSTEKYFFNLSSMTCEKFFSGGCHRNRIENRFPDEATCMGFC-----------------APKKIPSFCYSPKDEGLCSANVTRYYFNPRYRTCDAFTYTGCGGNDNNFVSREDC 204 HSP 2 Score: 72.4034 bits (176), Expect = 4.864e-13 Identity = 51/182 (28.02%), Postives = 83/182 (45.60%), Query Frame = 0
Query: 372 CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPR-CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHC 549
CL P+ G C+ Y +++ C ++Y GC G+ N F + EAC + C I+ P+ CR++ + + T + ++ C + GC + RI N F + C C + S P + G C ++ R+Y+ P TC +FT++GC GN NNF S + C
Sbjct: 36 CLLPLDYGPCRALLLRYYYDRYTQSCRQFLYGGCEGNANNFYTWEACDDACWRIEKVPKVCRLQVSVDD--QCEGSTEKYFFNLSSMTCEKFFSGGC-HRNRIENRFPDEATCMGFCAPKKIPSFCYSPK----------DEGLCSANVTRYYFNPRYRTCDAFTYTGCGGNDNNFVSREDC 204 HSP 3 Score: 71.633 bits (174), Expect = 1.034e-12 Identity = 43/132 (32.58%), Postives = 63/132 (47.73%), Query Frame = 0
Query: 501 EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICE-QPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSR--NNFPTRAQCEATCLNREVDAF 629
E CLL + G C L R+YY+ ++C F + GC GN NNF +++ C C E +C Q + C ++F+N S TCE+F GGC +R N FP A C C +++ +F
Sbjct: 34 EICLLPLDYGPCRALLLRYYYDRYTQSCRQFLYGGCEGNANNFYTWEACDDACWRIE--------KVPKVCRLQVSVDDQCEGSTEKYFFNLSSMTCEKFFSGGCHRNRIENRFPDEATCMGFCAPKKIPSF 157 HSP 4 Score: 62.3882 bits (150), Expect = 1.393e-9 Identity = 35/108 (32.41%), Postives = 50/108 (46.30%), Query Frame = 0
Query: 512 CHGSLPRFYYEPMDETCHSFTFSGCHGNG--NNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQC 617
C GS ++++ TC F GCH N N F C+ C ++ S C PK +G C + R+++NP TC+ F Y GC G+ NNF +R C
Sbjct: 106 CEGSTEKYFFNLSSMTCEKFFSGGCHRNRIENRFPDEATCMGFCAPKKI---------PSFCYSPKDEGLCSANVTRYYFNPRYRTCDAFTYTGCGGNDNNFVSREDC 204
BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Match: gi|82203423|sp|Q6T269.1|VKT3_BITGA (RecName: Full=Kunitz-type serine protease inhibitor bitisilin-3; AltName: Full=Two-Kunitz protease inhibitor) HSP 1 Score: 101.679 bits (252), Expect = 6.098e-24 Identity = 54/136 (39.71%), Postives = 71/136 (52.21%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKD------HMDPTMNSLGRHIFRV 364
C L GPC Y RF+Y+ + KCE F++GGC GN NNFK+ EEC TC E CI P G C +F++D S+ C F+Y GC GN NNF + EC+ TC + + P GR + R+
Sbjct: 18 CNLPADLGPCKNYTGRFYYDSASNKCEVFIYGGCPGNANNFKTREECRKTCVEI-------CILPAELGPCDEYTGRFYYDSASNKCEVFIYGGCQGNANNFKTRDECRKTCVEICILPAELGPCDEYTGRLLLRL 146 HSP 2 Score: 86.6557 bits (213), Expect = 1.212e-18 Identity = 47/125 (37.60%), Postives = 60/125 (48.00%), Query Frame = 0
Query: 498 FALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
F E C L + G C RFYY+ C F + GC GN NNF++ + C TC IC P GPC RF+Y+ + CE F YGGC+G+ NNF TR +C TC+
Sbjct: 13 FPAEFCNLPADLGPCKNYTGRFYYDSASNKCEVFIYGGCPGNANNFKTREECRKTC--------------VEICILPAELGPCDEYTGRFYYDSASNKCEVFIYGGCQGNANNFKTRDECRKTCV 123 HSP 3 Score: 68.5514 bits (166), Expect = 2.012e-12 Identity = 41/134 (30.60%), Postives = 59/134 (44.03%), Query Frame = 0
Query: 296 CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAP 428
C P G C +F++D S+ C F+Y GC GN NNF + EC+ TC + C+ P G C G + ++ N+C +IY GC G+ N F + + C+ C EI I P
Sbjct: 18 CNLPADLGPCKNYTGRFYYDSASNKCEVFIYGGCPGNANNFKTREECRKTC----------------------VEICILPAELGPCDEYTGRFYYDSASNKCEVFIYGGCQGNANNFKTRDECRKTCVEICILP 129
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|669323448|gb|KFD63705.1| (hypothetical protein M514_06583, partial [Trichuris suis]) HSP 1 Score: 219.164 bits (557), Expect = 4.486e-56 Identity = 154/490 (31.43%), Postives = 219/490 (44.69%), Query Frame = 0
Query: 212 TTLPSTTPAST--LSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKR------SLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC---EIQIAP--------------RCRIEH-TFEPCL----PSPSYTS--VWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAE--------VRADGS--------ILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETFHEESTSSGVVV 651
+T P PA+T + C G C R++YN EKCE F++ GC GN NNF S+ C+ TC E D C P G+C+G ++++FD + C +YSGCGGNGNNFDS A C+ C + +NS + VP + L+ C P G C + + +++ E C P+ Y+GCGG+ N F S E C N C + AP + H PC P P S W Y + MC GC+ N F + + C C P CL +RG C G R++Y+ +TC F + GC G GNNF + Q CL C +AE ++A+ + IL C+QPK++G C + + R+F+N + CE F Y GC G+RNNF T +QCE C R + + ++++ F ES S V
Sbjct: 420 STAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLELD---ADICKHPPNVGTCTGRIQRWYFDRPARKCKVLLYSGCGGNGNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGNGNNFESREVCMNTCPNAKKTTAPTTVATLPAMTTLPFKDDTSHLPPNPCFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGN---ANNFESFASCMVKC----------GPEILSSRCLHPTDRGDCSGHFVRYHYDSDSKTCKEFVYGGCGGTGNNFATKQECLRACANAEADDEVKKLLKAERTPVSIQPTLILPTEDSCKQPKMRGNCNKYELRYFFNAQVGRCEFFFYSGCGGNRNNFKTLSQCELFCSKRRPNPLLFGSSDDATAFSSESYGSTEAV 893 HSP 2 Score: 187.963 bits (476), Expect = 9.207e-46 Identity = 134/464 (28.88%), Postives = 198/464 (42.67%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC--------------------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSV---------------------WVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLE--VEPMFAL--------------EGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESET 639
C+ RGPC + +RFFY+ + +C F + GC GN NNFKSL +C+ C + P+ ++ C P GSC S ++++++ C F+YSGCGGN NNFDS+ CQ TC + +D + C P G C + F++ +C +YSGCGG+ N F S +C+ C +++ + +P +V W Y E + MC GC GN F + C TC + + + V + A+ C R + G C S R++Y+ CH FT+ GC GN NNF+SF C+ C IL+ S C P +G C R+ Y+ +TC+EF YGGC G+ NNF T+ +C C N E D ++ L + T
Sbjct: 364 CRFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLSDCQKFCIRGLKSTAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLE-LDADI-----------------CKHPPNVGTCTGRIQRWYFDRPARKCKVLLYSGCGGNGNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGN---GNNFESREVCMNTCPNAKKTTAPTTVATLPAMTTLPFKDDTSHLPPNPCFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGNANNFESFASCMVKCGP-------EILS--SRCLHPTDRGDCSGHFVRYHYDSDSKTCKEFVYGGCGGTGNNFATKQECLRACANAEADDEVKKLLKAERT 797 HSP 3 Score: 176.022 bits (445), Expect = 7.977e-42 Identity = 115/368 (31.25%), Postives = 165/368 (44.84%), Query Frame = 0
Query: 291 EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHM-----DPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPM-------------FALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA-EVRADGSILTNASICEQP----------------KLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
E + C P +G C +F +D + C F YSGCGGN NNF SL++CQ C + D + R + P + + C P +G C+ + +N E +C P+IYSGCGG+ N F S C+ C A C+ C W + R C V + GC GN F + + CER CL + S + +P+ L C GSC G R+YY+ ++E C FT++GC GNGNNF+S + C+ TC +A + A ++ T ++ P + GPCR R+FY+ + C F YGGC G+ NNF + A C C
Sbjct: 359 EDESICRFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLSDCQKFCIRGLKSTAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLELDADICKHPPNVGTCT---GRIQRWYFDRPARKCKVLLYSGCGGN---GNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGNGNNFESREVCMNTCPNAKKTTAPTTVATLPAMTTLPFKDDTSHLPPNPCFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGNANNFESFASCMVKC 720 HSP 4 Score: 140.969 bits (354), Expect = 1.173e-30 Identity = 122/431 (28.31%), Postives = 174/431 (40.37%), Query Frame = 0
Query: 27 ERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPL------RPQGFSKSHPKLM----------HPIQVDRNEAITITLSLKLLRIESWP------GRRNNSF----IVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPE-KDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCK-----DHMDPTMNSLGRHIFRVPKRSL---SDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
+ G C +R+++N CE F +SGCG N NNF C+ C+ + +I + P ++ T + + D +++ Y+ G N S R C L +P S K++ HP V L P G N+F + MN ++ P+T+AT TTLP TS L +PC GPC R+FY+ K C F +GGC GN NNF+S C C PE C+ P +G CSG ++H+D S TC +FVY GCGG GNNF + EC C D + + + + P L C +P G+C + Y FN +C + YSGCGG+ N F + C+ C
Sbjct: 450 DAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLE---LDADICKHPP--NVGTCTGRIQRWYFDRPARKCKVLLYSGCGGNGNNFDSQASCERYCLPELSSDVNSQPVKVSMVPHKVLPTVLPLNRCFHPKNVGSCTGQYHRWYYDELEEMCKPFTYTGCGGNGNNFESREVCMN--TCPNAKKTTAPTTVATLPAMTTLP-------FKDDTSHLPPNPCFHRRDPGPCRASHRRWFYDSKVNMCHPFTYGGCAGNANNFESFASCMVKCG---PEILSSRCLHPTDRGDCSGHFVRYHYDSDSKTCKEFVYGGCGGTGNNFATKQECLRACANAEADDEVKKLLKAERTPVSIQPTLILPTEDSCKQPKMRGNCNKYELRYFFNAQVGRCEFFFYSGCGGNRNNFKTLSQCELFC 863 HSP 5 Score: 94.7449 bits (234), Expect = 4.084e-16 Identity = 79/284 (27.82%), Postives = 115/284 (40.49%), Query Frame = 0
Query: 358 GRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG-CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA--EVRADGSILTNASI-----------CEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
G+ + R CL+ G C + + F+ + +C IY GCGG+ N F S E C+ +C +A + F P ++SV +T C +G + S C + ++ L +F E C +RG C RF+Y+ + C F +SGC GN NNF+S C C D T A C P+ G C+ R++YN E CE F Y GC G+ NNF + C+ TCL + D
Sbjct: 232 GQEVAAPVYRLDEKCLEVPEPGPCDDQLERWHFDAEDRRCKILIYGGCGGNSNNFPSKEICEKKCNAAVA-----ANRF-----CPDWSSVLTDENGVTFECLTKTCP----LGYKCFISGNEGYCCANADTNDLAAPLLFEDESICRFPKDRGPCGNFDLRFFYDAEEGECRYFFYSGCGGNRNNFKSLSDCQKFCIRGLKSTAPDDVPATTARFVPDPPPKVENKCNFPRDAGSCKESHQRWYYNTVEEKCEPFIYSGCGGNPNNFDSMLGCQETCLELDAD 501 HSP 6 Score: 77.7962 bits (190), Expect = 6.365e-11 Identity = 48/112 (42.86%), Postives = 66/112 (58.93%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
C RGPC +L R+++N KCE+F++GGC+GN NNF + +C C K D C P G C G +++FD C +F+Y GCGGNGNNF +L EC++TC
Sbjct: 1056 CDYPKERGPCSGHLRRWYWNKVRSKCESFLYGGCRGNANNFGTEAKCLAEC---KGLPADVCQHPKDVGVCKGYFRRWYFDSSVGWCKQFIYGGCGGNGNNFATLQECRTTC 1164 HSP 7 Score: 73.9442 bits (180), Expect = 9.673e-10 Identity = 112/467 (23.98%), Postives = 170/467 (36.40%), Query Frame = 0
Query: 28 RGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESW-----PGRRNNSFIVM-----NDLVVLQTNQSLRPSTIATDSTTTTLPSTT-PASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERT 483
RG+C YE RYFFN G CE F +SGCG N NNF+ CE C RR L D D + SY S A ++SP + L + P P+ + + +L E+ PG S + L LQT+ +P++IAT+ T+T P ++ + A P +L + G + G G +F+ HK C P +G CSG +++++ C F+Y GC GN NNF + A+C + CK L + C P G C+ + F+ + C +IY GCGG+ N F + + C+ C ++ R +++ P P + + V+ T +G +T SE RT
Sbjct: 819 RGNCNKYELRYFFNAQVGRCEFFFYSGCGGNRNNFKTLSQCELFCSK-------------RRPNPLLFGSSD------DATAFSSESYGSTEA----VHSPSANAYPPGSSL--PSDHREEPSTSSPVTQYSGRLQSSSTTLSGTEKETSYTFLPPGYTEGSLLSQYPGNRPSLPGLQTHHVKKPASIATNRPTSTYGGPVGPDASQQAKEDVSAYEPGELPLQPGVSETTAKAKPPVSPAPPPMPPGSAGVAEAGTSFR-----------HK------CDYPKERGPCSGHLRRWYWNKVRSKCESFLYGGCRGNANNFGTEAKCLAECK--------GLPADV----------CQHPKDVGVCKGYFRRWYFDSSVGWCKQFIYGGCGGNGNNFATLQECRTTC---LSGRTKVDDATAEYAPLPPAGADLSNYGREHAGTVSTTSVGTAGLGVYGTTISEYVRT 1222 HSP 8 Score: 72.0182 bits (175), Expect = 3.505e-9 Identity = 63/204 (30.88%), Postives = 89/204 (43.63%), Query Frame = 0
Query: 508 ERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREV---DAFIQF--LAEESETFHEESTSSGVVVAEIFIGIALSGLVILGVYFGFRLILEQVLVKEMQSHGSGLVFSDHFGQKIELTDS 706
ERG C G L R+Y+ + C SF + GC GN NNF + CLA C A +C+ PK G C+ R++++ S+ C++F YGGC G+ NNF T +C TCL+ DA ++ L V+ +G A GL + G I E V Q+ G G S+H + S
Sbjct: 1061 ERGPCSGHLRRWYWNKVRSKCESFLYGGCRGNANNFGTEAKCLAECKGLP----------ADVCQHPKDVGVCKGYFRRWYFDSSVGWCKQFIYGGCGGNGNNFATLQECRTTCLSGRTKVDDATAEYAPLPPAGADLSNYGREHAGTVSTTSVGTA--GLGVYGT-----TISEYV---RTQTGGYGTPESEHVVHTVSTIPS 1244
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|730370974|gb|KHJ42567.1| (Kunitz/Bovine pancreatic trypsin inhibitor domain protein [Trichuris suis]) HSP 1 Score: 215.312 bits (547), Expect = 1.891e-55 Identity = 120/402 (29.85%), Postives = 196/402 (48.76%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-----EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLE-VEP-MFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
+PC+L GPC R++Y+W ++C F +GGC+GN NN+ S+EECE TC + + ++ +PC P G C K++++ +S C FVY GC GN N FD+L +C++ C + + + C+ P SG C+ + Y ++ +CV + Y GC G+ N + S + C+ C E ++ C + PC+ +Y + + Y++E + C V GC N F T ECE C + + EP + A + C+L E G C + ++Y++ + C F + GC GN N F + + C C + + + IC P G C RFF++ + C+ F Y GC+G+ NNF T +C A C++ +
Sbjct: 552 NPCKLPAETGPCRGNFPRYYYDWDLQQCLEFTYGGCEGNANNYDSVEECEATCPDGEHKEQNPCELPADSGPCMAYFTKYYYNKESKKCETFVYGGCAGNENRFDTLEDCEAVCIEEEE------------------NPCILPADSGPCKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPEPKEEPKVENPCELPSDSGPCM---AYFTKYYYNKETKKCETFVYGGCQGN---ANRFDTLEECEAKCAEGKKEDVSPAEPDVPAGDACILPKETGPCKQVIKKWYFDSKKKVCRIFIYGGCKGNANKFDTKEECQQRC--------VATMPASVICRLPYAMGSCSLSMKRFFFDFEAKACKSFTYTGCEGNANNFRTEEECSAKCISAQ 921 HSP 2 Score: 159.844 bits (403), Expect = 6.047e-37 Identity = 97/338 (28.70%), Postives = 154/338 (45.56%), Query Frame = 0
Query: 291 EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPS-PSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDA---EVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
++++PC P G C G+ ++++D+ C +F Y GC GN NN+DS+ EC++TC D G H + P C P SG C Y +N+ +C ++Y GC G++N F + E C+ C + C + PC P Y W + + CV GC N +++ ECE TC + P C L + G C ++YY + C +F + GC GN N F + + C A C + +V + C PK GPC++ +++++ + C F YGGCKG+ N F T+ +C+ C+
Sbjct: 549 QEENPCKLPAETGPCRGNFPRYYYDWDLQQCLEFTYGGCEGNANNYDSVEECEATCPD---------GEHKEQNP------CELPADSGPCMAYFTKYYYNKESKKCETFVYGGCAGNENRFDTLEDCEAVCIEEEENPCILPADSGPCKGKFPRYYYDW----DLKKCVEFTYGGCEGN---ANNYASLQECEATCPEPKEEPKVENP------CELPSDSGPCMAYFTKYYYNKETKKCETFVYGGCQGNANRFDTLEECEAKCAEGKKEDVSPAEPDVPAGDACILPKETGPCKQVIKKWYFDSKKKVCRIFIYGGCKGNANKFDTKEECQQRCV 858 HSP 3 Score: 121.324 bits (303), Expect = 1.555e-24 Identity = 95/332 (28.61%), Postives = 139/332 (41.87%), Query Frame = 0
Query: 15 EHVIPEFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPRRSLKNLTLDFDQGFEDWDNYDWQIISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTC 346
EH C L + G C Y +Y++N+ S CETF + GC NEN F + CE +C+ EEE P S F + + DWD +Y N S +C P K PK+ +P ++ + + K + ++ +F+ Q N + R T+ S AG C L GPC + +++++ K + C F++GGCKGN N F + EEC+ C P C P GSCS S ++F FDF++ C F Y+GC GN NNF + EC + C
Sbjct: 608 EHKEQNPCELPADSGPCMAYFTKYYYNKESKKCETFVYGGCAGNENRFDTLEDCEAVCIE----EEENPCILPADS-GPCKGKFPRYYYDWDLKKCVEFTYGGCEGNANNYASLQECEATCPEP-------KEEPKVENPCELPSDSGPCMAYFTKYYYNKE--TKKCETFVYGG----CQGNAN-RFDTLEECEAKCAEGKKEDVSPAEPDVP--AGDACILPKETGPCKQVIKKWYFDSKKKVCRIFIYGGCKGNANKFDTKEECQQRCVATMP-ASVICRLPYAMGSCSLSMKRFFFDFEAKACKSFTYTGCEGNANNFRTEEECSAKC 917
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|749753431|ref|XP_011139107.1| (PREDICTED: papilin isoform X2 [Harpegnathos saltator]) HSP 1 Score: 215.698 bits (548), Expect = 6.486e-55 Identity = 133/414 (32.13%), Postives = 194/414 (46.86%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSP--SYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEV--EPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETF 640
+ C L GPC Y ++Y+ ++C FV+GGC GN N FK+ EECE C P+ DPC G C+G+ +++F+ +S C +FVY GC NGNNF + C C P R C P G C +Q + F+Q+EN+C+P+ Y+GCGG+ N F S EAC++ C +I + P LP +YT W Y ++ C + GC + N F T +C C++ + E F E C L E G C G +++Y+ D C F + GC NGNNF + + C C + + +C PK+ GPC +F+Y+ ++C EF Y GC+G+RN F R CEA C + +A + T
Sbjct: 1875 AACFLPKIAGPCKGYQPTWYYDADRKQCGQFVYGGCLGNANKFKTKEECEELCV--TPDGVDPCEQLKEIGPCAGNFTRWYFNAESQNCEQFVYGGCKANGNNFPTEIACHQQCLQ----------------PGRRRDTCTLPRAEGTCTEKQSRWYFDQSENRCMPFYYTGCGGNRNNFESREACESDCPAKI----EQDSCLLPALPGDCHNYTQRWYYDSYEQHCRLFYYGGCGGNE---NNFETEHDCVNRCVTAVTTPAPPGRELEFRTEFCFLPDEHGPCSGDQAKWFYDSRDGLCRQFLYGGCQSNGNNFNTREECEYRCGEVQ-----------DLCTLPKVVGPCNGFVQQFYYDRRADSCYEFEYSGCQGNRNRFQDRESCEAKCKRQPPPPTPSPIARITTTL 2252 HSP 2 Score: 171.4 bits (433), Expect = 2.651e-40 Identity = 127/432 (29.40%), Postives = 184/432 (42.59%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDP----------CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-----CRIEHTFEPCLPSPS---------------YTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC-HDAEVRADG--SILTNASICEQPKLKGPCRR-DQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL--NREVDA 628
C L G C Y R++Y+ + C F +GGC GN NNF++ +C C P C P G CSG K+ +D + C +F+Y GC NGNNF++ EC+ C + D C P G C + +++ + C + YSGC G+ N F E+C+ +C+ Q P RI T P + P + + Y +Q MC + GC N+F T +CER C + H +V C L + G C G+ ++YYEP +C FT+ GC GN N F + C + C H E G + ++N SIC++P G C RF+++ + C F Y GC G+RN F T C TCL + E+D
Sbjct: 2055 CLLPALPGDCHNYTQRWYYDSYEQHCRLFYYGGCGGNENNFETEHDCVNRCVTAVTTPAPPGRELEFRTEFCFLPDEHGPCSGDQAKWFYDSRDGLCRQFLYGGCQSNGNNFNTREECEYRCGEVQDL-------------------CTLPKVVGPCNGFVQQFYYDRRADSCYEFEYSGCQGNRNRFQDRESCEAKCKRQPPPPTPSPIARITTTLAPSIEEPKSPICSAPADPGDCDGTITAYYYDAQQHMCQAFIYGGCGGN---ANKFQTEEQCERLC--GKFHGQDV--------CNLPVDSGPCRGAFRKYYYEPSTRSCREFTYGGCDGNANRFSTIPECESICIHQEEPVPPGNDTSVSNLSICKEPVDSGSCTSGGTKRFYFDEEHQACRAFIYTGCGGNRNRFKTFESCINTCLRTSNEIDV 2454 HSP 3 Score: 170.629 bits (431), Expect = 4.193e-40 Identity = 117/409 (28.61%), Postives = 178/409 (43.52%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHI-FRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVV--TVGCSNTKRIGNEFSTSSECERTC---------------LSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C L A G C R++++ +C F + GC GN NNF+S E CE+ C + D C+ P L G C +++++D C F Y GCGGN NNF++ +C + C + T GR + FR C P G C +Q + ++ + C ++Y GC + N F + E C+ RC ++ C + PC + + Y C GC + N F CE C + + S+E EP + C + G C G++ +YY+ C +F + GC GN N FQ+ + C C +C P GPCR +++Y PS +C EF YGGC G+ N F T +CE+ C+++E
Sbjct: 1996 CTLPRAEGTCTEKQSRWYFDQSENRCMPFYYTGCGGNRNNFESREACESDCPAKI--EQDSCLLPALPGDCHNYTQRWYYDSYEQHCRLFYYGGCGGNENNFETEHDCVNRCVTAVT-TPAPPGRELEFRT-----EFCFLPDEHGPCSGDQAKWFYDSRDGLCRQFLYGGCQSNGNNFNTREECEYRCG-EVQDLCTLPKVVGPCN---GFVQQFYYDRRADSCYEFEYSGCQGNR---NRFQDRESCEAKCKRQPPPPTPSPIARITTTLAPSIE-EPKSPI--CSAPADPGDCDGTITAYYYDAQQHMCQAFIYGGCGGNANKFQTEEQCERLC---------GKFHGQDVCNLPVDSGPCRGAFRKYYYEPSTRSCREFTYGGCDGNANRFSTIPECESICIHQE 2377 HSP 4 Score: 118.627 bits (296), Expect = 1.877e-23 Identity = 64/203 (31.53%), Postives = 104/203 (51.23%), Query Frame = 0
Query: 221 STLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQL-EQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
+TL+ S E C G CD + ++Y+ + C+ F++GGC GN N F++ E+CE C + + D C P+ G C G+ K++++ + +C +F Y GC GN N F ++ EC+S C +P + +LS C +P+ SG C + F++ C +IY+GCGG+ N F + E+C N C
Sbjct: 2250 TTLAPSIEEPKSPICSAPADPGDCDGTITAYYYDAQQHMCQAFIYGGCGGNANKFQTEEQCERLCGKFHGQ--DVCNLPVDSGPCRGAFRKYYYEPSTRSCREFTYGGCDGNANRFSTIPECESICIHQEEPVPPGNDTSV-----SNLSICKEPVDSGSCTSGGTKRFYFDEEHQACRAFIYTGCGGNRNRFKTFESCINTC 2445 HSP 5 Score: 91.2781 bits (225), Expect = 5.050e-15 Identity = 58/180 (32.22%), Postives = 79/180 (43.89%), Query Frame = 0
Query: 445 YTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
+T W Y + C GC + N F T EC+ C+ + + C L G C G P +YY+ + C F + GC GN N F++ + C C V DG CEQ K GPC + R+++N + CE+F YGGCK + NNFPT C CL
Sbjct: 1830 FTVKWYYDTDYGGCSRFWYGGCKGNE---NRFKTQEECKEVCVQPKGKA----------ACFLPKIAGPCKGYQPTWYYDADRKQCGQFVYGGCLGNANKFKTKEECEELC----VTPDG-----VDPCEQLKEIGPCAGNFTRWYFNAESQNCEQFVYGGCKANGNNFPTEIACHQQCL 1987
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|1126587719|ref|XP_019696889.1| (PREDICTED: papilin isoform X1 [Harpegnathos saltator]) HSP 1 Score: 215.312 bits (547), Expect = 1.023e-54 Identity = 133/412 (32.28%), Postives = 193/412 (46.84%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSP--SYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEV--EPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESETF 640
C L GPC Y ++Y+ ++C FV+GGC GN N FK+ EECE C P+ DPC G C+G+ +++F+ +S C +FVY GC NGNNF + C C P R C P G C +Q + F+Q+EN+C+P+ Y+GCGG+ N F S EAC++ C +I + P LP +YT W Y ++ C + GC + N F T +C C++ + E F E C L E G C G +++Y+ D C F + GC NGNNF + + C C + + +C PK+ GPC +F+Y+ ++C EF Y GC+G+RN F R CEA C + +A + T
Sbjct: 1314 CFLPKIAGPCKGYQPTWYYDADRKQCGQFVYGGCLGNANKFKTKEECEELCV--TPDGVDPCEQLKEIGPCAGNFTRWYFNAESQNCEQFVYGGCKANGNNFPTEIACHQQCLQ----------------PGRRRDTCTLPRAEGTCTEKQSRWYFDQSENRCMPFYYTGCGGNRNNFESREACESDCPAKI----EQDSCLLPALPGDCHNYTQRWYYDSYEQHCRLFYYGGCGGNE---NNFETEHDCVNRCVTAVTTPAPPGRELEFRTEFCFLPDEHGPCSGDQAKWFYDSRDGLCRQFLYGGCQSNGNNFNTREECEYRCGEVQ-----------DLCTLPKVVGPCNGFVQQFYYDRRADSCYEFEYSGCQGNRNRFQDRESCEAKCKRQPPPPTPSPIARITTTL 1689 HSP 2 Score: 171.014 bits (432), Expect = 3.859e-40 Identity = 127/432 (29.40%), Postives = 184/432 (42.59%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDP----------CISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-----CRIEHTFEPCLPSP---------------SYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC-HDAEVRADG--SILTNASICEQPKLKGPCRR-DQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL--NREVDA 628
C L G C Y R++Y+ + C F +GGC GN NNF++ +C C P C P G CSG K+ +D + C +F+Y GC NGNNF++ EC+ C + D C P G C + +++ + C + YSGC G+ N F E+C+ +C+ Q P RI T P + P + + Y +Q MC + GC N+F T +CER C + H +V C L + G C G+ ++YYEP +C FT+ GC GN N F + C + C H E G + ++N SIC++P G C RF+++ + C F Y GC G+RN F T C TCL + E+D
Sbjct: 1492 CLLPALPGDCHNYTQRWYYDSYEQHCRLFYYGGCGGNENNFETEHDCVNRCVTAVTTPAPPGRELEFRTEFCFLPDEHGPCSGDQAKWFYDSRDGLCRQFLYGGCQSNGNNFNTREECEYRCGEVQDL-------------------CTLPKVVGPCNGFVQQFYYDRRADSCYEFEYSGCQGNRNRFQDRESCEAKCKRQPPPPTPSPIARITTTLAPSIEEPKSPICSAPADPGDCDGTITAYYYDAQQHMCQAFIYGGCGGN---ANKFQTEEQCERLC--GKFHGQDV--------CNLPVDSGPCRGAFRKYYYEPSTRSCREFTYGGCDGNANRFSTIPECESICIHQEEPVPPGNDTSVSNLSICKEPVDSGSCTSGGTKRFYFDEEHQACRAFIYTGCGGNRNRFKTFESCINTCLRTSNEIDV 1891 HSP 3 Score: 170.244 bits (430), Expect = 5.731e-40 Identity = 117/409 (28.61%), Postives = 178/409 (43.52%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHI-FRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVV--TVGCSNTKRIGNEFSTSSECERTC---------------LSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C L A G C R++++ +C F + GC GN NNF+S E CE+ C + D C+ P L G C +++++D C F Y GCGGN NNF++ +C + C + T GR + FR C P G C +Q + ++ + C ++Y GC + N F + E C+ RC ++ C + PC + + Y C GC + N F CE C + + S+E EP + C + G C G++ +YY+ C +F + GC GN N FQ+ + C C +C P GPCR +++Y PS +C EF YGGC G+ N F T +CE+ C+++E
Sbjct: 1433 CTLPRAEGTCTEKQSRWYFDQSENRCMPFYYTGCGGNRNNFESREACESDCPAKI--EQDSCLLPALPGDCHNYTQRWYYDSYEQHCRLFYYGGCGGNENNFETEHDCVNRCVTAVT-TPAPPGRELEFRT-----EFCFLPDEHGPCSGDQAKWFYDSRDGLCRQFLYGGCQSNGNNFNTREECEYRCG-EVQDLCTLPKVVGPCN---GFVQQFYYDRRADSCYEFEYSGCQGNR---NRFQDRESCEAKCKRQPPPPTPSPIARITTTLAPSIE-EPKSPI--CSAPADPGDCDGTITAYYYDAQQHMCQAFIYGGCGGNANKFQTEEQCERLCGK---------FHGQDVCNLPVDSGPCRGAFRKYYYEPSTRSCREFTYGGCDGNANRFSTIPECESICIHQE 1814 HSP 4 Score: 118.242 bits (295), Expect = 2.118e-23 Identity = 64/203 (31.53%), Postives = 104/203 (51.23%), Query Frame = 0
Query: 221 STLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQL-EQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
+TL+ S E C G CD + ++Y+ + C+ F++GGC GN N F++ E+CE C + + D C P+ G C G+ K++++ + +C +F Y GC GN N F ++ EC+S C +P + +LS C +P+ SG C + F++ C +IY+GCGG+ N F + E+C N C
Sbjct: 1687 TTLAPSIEEPKSPICSAPADPGDCDGTITAYYYDAQQHMCQAFIYGGCGGNANKFQTEEQCERLCGKFHGQ--DVCNLPVDSGPCRGAFRKYYYEPSTRSCREFTYGGCDGNANRFSTIPECESICIHQEEPVPPGNDTSV-----SNLSICKEPVDSGSCTSGGTKRFYFDEEHQACRAFIYTGCGGNRNRFKTFESCINTC 1882 HSP 5 Score: 90.8929 bits (224), Expect = 5.948e-15 Identity = 58/180 (32.22%), Postives = 79/180 (43.89%), Query Frame = 0
Query: 445 YTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
+T W Y + C GC + N F T EC+ C+ + + C L G C G P +YY+ + C F + GC GN N F++ + C C V DG CEQ K GPC + R+++N + CE+F YGGCK + NNFPT C CL
Sbjct: 1267 FTVKWYYDTDYGGCSRFWYGGCKGNE---NRFKTQEECKEVCVQPKGKA----------ACFLPKIAGPCKGYQPTWYYDADRKQCGQFVYGGCLGNANKFKTKEECEELC----VTPDG-----VDPCEQLKEIGPCAGNFTRWYFNAESQNCEQFVYGGCKANGNNFPTEIACHQQCL 1424
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|6164595|gb|AAF04457.1|AF078161_1 (lacunin [Manduca sexta]) HSP 1 Score: 215.312 bits (547), Expect = 1.034e-54 Identity = 133/398 (33.42%), Postives = 191/398 (47.99%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR----CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFA---LEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
+ C L RG C Y + ++Y+ + C F +GGC+GNGN F + EECE C + P+ D C P +KG+C G ++++D + CS+F+Y GC GN NN+ SL CQ C+ P+RS C PI G C G +GFN +C +++ GC G+ N F S AC RC+ P+ C + C Y W Y ++ C GC+ GN F+T +ECE C S+ + V P A E C + + G C + R+ Y+ C +F + GC GN NNF + ++C C A+ IC+ P GPC R+FY+PS ++C +F YGGC G+ N F TR CE+ C
Sbjct: 1953 AACGLPHDRGTCRNYSVYWYYDLEYGGCSRFWYGGCEGNGNKFATKEECEDVCVQPAPK--DACNLPKVKGACLGYNIRWYYDAEQEQCSQFIYGGCLGNANNYASLQLCQEQCQ-----------------PERSEDQCRLPIDRGSCSGNFGRWGFNSETRRCEQFMWGGCEGNTNRFSSEIACIQRCDPPGKPKLPDLCTLPAAIGDCA---DYRERWYYDTREKSCQRFYYGGCAGN---GNNFATQAECEGRC-SEAKITTTVRPTEAHPLTEMCFMEKDPGPCTDTETRWVYDYKLGKCVTFEYGGCGGNRNNFPTEEYCQYYCGTAQ-----------DICQLPMRSGPCTESLMRWFYDPSSDSCSQFTYGGCDGNDNRFETRDDCESRC 2313 HSP 2 Score: 129.413 bits (324), Expect = 7.436e-27 Identity = 109/419 (26.01%), Postives = 175/419 (41.77%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIF-----RVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGS---DNLFLSHEACKNRC-EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTN---------------------ASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C + GPC R+ Y++K KC F +GGC GN NNF + E C+ C + D C P+ G C+ S ++ +D S +CS+F Y GC GN N F++ +C+S C+ T + + R+ ++ ++C + CQ V+ +Q +CV + G S +F + EAC+ C + CR + PC +Y + + + + R C GC N FST EC+ C S+ ++ C E G C +YY+ + +TC F + GCHGN N F++ + C C + A C P+ PC + ++Y+ +C + GGC+ + N+F T +CE C
Sbjct: 2206 CFMEKDPGPCTDTETRWVYDYKLGKCVTFEYGGCGGNRNNFPTEEYCQYYCGTAQ----DICQLPMRSGPCTESLMRWFYDPSSDSCSQFTYGGCDGNDNRFETRDDCESRCRTGSAQTTTTPTTSVTVAVDSRIAEQLPTECHISPSEEPCQRSGQVWYLDQRLGECVSHDNLDSGDSCRHTGVFPTQEACERTCGAFRNINVCRYDLDPGPCR---TYEAKYFFDKASRSCREFAYGGCHGGP---NRFSTIDECQEVCRSE------------MDPCKQVVEPGDCTSRYVMWYYDNVRDTCLQFIYGGCHGNENRFETLEDCERKCRQRPESVSTTTAFIATTTTTTTLAPTLPPTEAEPIAEECRTPESLVPCGANITTYYYDQESRSCVQGDIGGCRYA-NSFRTEEECERRC 2601 HSP 3 Score: 125.561 bits (314), Expect = 1.305e-25 Identity = 112/443 (25.28%), Postives = 178/443 (40.18%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC-----------------------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSL-----------------------EVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAF 629
CQL M GPC L+R+FY+ ++ C F +GGC GN N F++ ++CE+ C +E P + ISP + C SG+ ++ D + C +N DS C+ T + PT + R R+++ C + G C+ + Y F++ C + Y GC G N F + + C+ C ++ P + EP + Y +W Y + C+ + GC + N F T +CER C R S+ E EP+ E C C ++ +YY+ +C GC N+F++ + C C + ++CE GPCR P+F++ P+ +C ++ YGGC G N F T +CE C N D
Sbjct: 2263 CQLPMRSGPCTESLMRWFYDPSSDSCSQFTYGGCDGNDNRFETRDDCESRCRTGSAQTTTTPTTSVTVAVDSRIAEQLPTECH--ISPSEE-PCQRSGQVWYLDQRLGECVSH---------DNLDSGDSCRHT---GVFPTQEACERTCGAF--RNINVCRYDLDPGPCRTYEAKYFFDKASRSCREFAYGGCHGGPNRFSTIDECQEVCRSEMDP---CKQVVEPGDCTSRYV-MWYYDNVRDTCLQFIYGGCHGNE---NRFETLEDCERKCRQ-RPESVSTTTAFIATTTTTTTLAPTLPPTEAEPI--AEECRTPESLVPCGANITTYYYDQESRSCVQGDIGGCR-YANSFRTEEECERRC---------GAFRDINVCEARLDPGPCRSSIPKFYWEPASGSCLQYSYGGCGGGANRFSTIEECEEICGNTGPDVV 2668 HSP 4 Score: 120.168 bits (300), Expect = 5.000e-24 Identity = 94/373 (25.20%), Postives = 150/373 (40.21%), Query Frame = 0
Query: 217 TTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKC---ENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQ-------------------------------IAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVV--TVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
T+ + S +E + C ++ + PC ++ + + +C +N G + F + E CE TC + + C L G C K+ FD S +C +F Y GC G N F ++ ECQ C+ MDP C + + GDC ++ ++ + C+ +IY GC G++N F + E C+ +C + IA CR + PC + + + Y +E R CV GC R N F T ECER C + R ++ C R + G C S+P+FY+EP +C +++ GC G N F + + C C
Sbjct: 2326 TSVTVAVDSRIAEQLPTECHISPSEEPCQRSGQVWYLDQRLGECVSHDNLDSGDSCRHTGVFPTQEACERTCGAFR--NINVCRYDLDPGPCRTYEAKYFFDKASRSCREFAYGGCHGGPNRFSTIDECQEVCRSEMDP-------------------CKQVVEPGDCTSRYVMWYYDNVRDTCLQFIYGGCHGNENRFETLEDCERKCRQRPESVSTTTAFIATTTTTTTLAPTLPPTEAEPIAEECRTPESLVPC---GANITTYYYDQESRSCVQGDIGGC----RYANSFRTEEECERRCGAFRDINV----------CEARLDPGPCRSSIPKFYWEPASGSCLQYSYGGCGGGANRFSTIEECEEIC 2660 HSP 5 Score: 103.99 bits (258), Expect = 6.106e-19 Identity = 74/272 (27.21%), Postives = 117/272 (43.01%), Query Frame = 0
Query: 364 VPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR--CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQ 631
+P+ + C P G C+ + ++ C + Y GC G+ N F + E C++ C +Q AP+ C + CL Y W Y EQ C + GC N +++ C+ C +RS + C L +RGSC G+ R+ + C F + GC GN N F S C+ C +C P G C + R++Y+ ++C+ F YGGC G+ NNF T+A+CE C ++ ++
Sbjct: 1947 LPENKQAACGLPHDRGTCRNYSVYWYYDLEYGGCSRFWYGGCEGNGNKFATKEECEDVC-VQPAPKDACNLPKVKGACL---GYNIRWYYDAEQEQCSQFIYGGCLGN---ANNYASLQLCQEQCQPERSE----------DQCRLPIDRGSCSGNFGRWGFNSETRRCEQFMWGGCEGNTNRFSSEIACIQRCDPP------GKPKLPDLCTLPAAIGDCADYRERWYYDTREKSCQRFYYGGCAGNGNNFATQAECEGRCSEAKITTTVR 2195
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|951556759|ref|XP_014477805.1| (PREDICTED: papilin isoform X1 [Dinoponera quadriceps]) HSP 1 Score: 214.927 bits (546), Expect = 1.212e-54 Identity = 131/400 (32.75%), Postives = 193/400 (48.25%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSP--SYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEV--EPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDA 628
C L GPC Y ++++ + ++C FV+GGC GN N FK+ EECE C P+ DPC P G C+G+ +++F+ +S C +FVY GC NGNNF + C C P R C P T G C +Q + F+Q+EN+C+P+ Y+GCG ++N F S EAC++ C +I + P LP +YT W Y ++ C + GC + N F T +C C++ + + F E C LR E G C +++Y+ C F + GC NGNNF S + C C + + +C PK+ GPC +F+Y+ ++C EF YGGC+G++N F R CE C +E A
Sbjct: 1866 CFLPKIAGPCQGYHPTWYFDAERKQCGQFVYGGCLGNANKFKTKEECEELCV--TPDGIDPCELPKETGPCAGNFTRWYFNAESQDCEQFVYGGCKANGNNFPTEIACHQQCLQ----------------PGRRRDTCTLPRTEGTCTEKQSRWYFDQSENRCMPFYYTGCGSNENNFESREACESDCPAKI----EQDTCLLPALPGECHNYTHRWYYDSYEQQCRLFYYGGCGGNE---NNFETEHDCVNRCVTVVTTPTPPGKQAEFRREFCFLRDEHGPCTEDQVKWFYDSSVGVCRQFLYGGCQSNGNNFNSHEECEYRCGEVQ-----------DLCSLPKVIGPCDGYVKQFYYDRRTDSCYEFEYGGCQGNKNRFQDRESCEIKCRRQEPPA 2229 HSP 2 Score: 176.792 bits (447), Expect = 5.027e-42 Identity = 122/415 (29.40%), Postives = 180/415 (43.37%), Query Frame = 0
Query: 234 PCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-------------CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGS---ILTNAS---------ICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
PC+L GPC R+++N +++ CE FV+GGCK NGNNF + C C + + D C P +G+C+ +++FD + C F Y+GCG N NNF+S C+S C ++ CL P G+C + ++ E QC + Y GCGG++N F + C NRC + C + PC W Y +C + GC + GN F++ ECE C EV+ + +L + G C G + +FYY+ ++C+ F + GC GN N FQ + C C E A + I+T + IC P G C ++Y+ C+ F YGGC G+ N F T QCE C
Sbjct: 1924 PCELPKETGPCAGNFTRWYFNAESQDCEQFVYGGCKANGNNFPTEIACHQQCLQPG-RRRDTCTLPRTEGTCTEKQSRWYFDQSENRCMPFYYTGCGSNENNFESREACESDCPAKIE-----------------QDTCLLPALPGECHNYTHRWYYDSYEQQCRLFYYGGCGGNENNFETEHDCVNRCVTVVTTPTPPGKQAEFRREFCFLRDEHGPCTED---QVKWFYDSSVGVCRQFLYGGCQSN---GNNFNSHEECEYRC-------GEVQDLCSLPKVI-----GPCDGYVKQFYYDRRTDSCYEFEYGGCQGNKNRFQDRESCEIKCRRQEPPAPSTTAQIVTTPTPSVEEPKSPICLAPADPGNCDGTITSYYYDAQQRMCQAFIYGGCGGNANKFQTEEQCERLC 2302 HSP 3 Score: 167.548 bits (423), Expect = 4.485e-39 Identity = 115/404 (28.47%), Postives = 171/404 (42.33%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVV--TVGCSNTKRIGNEFSTSSECERTC------LSDRSHSLEVEPMFALEG-----CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C L G C R++++ +C F + GC N NNF+S E CE+ C + D C+ P L G C ++++D C F Y GCGGN NNF++ +C + C + + FR R C G C +Q + ++ + C ++Y GC + N F SHE C+ RC ++ C + PC Y + Y C GC K N F CE C + + P ++E CL + G+C G++ +YY+ C +F + GC GN N FQ+ + C C +C P GPCR +++Y S TC EF YGGC G+ N F T +CE+ C++ E
Sbjct: 1985 CTLPRTEGTCTEKQSRWYFDQSENRCMPFYYTGCGSNENNFESREACESDCPAKI--EQDTCLLPALPGECHNYTHRWYYDSYEQQCRLFYYGGCGGNENNFETEHDCVNRCVTVVTTPTPPGKQAEFR---REF--CFLRDEHGPCTEDQVKWFYDSSVGVCRQFLYGGCQSNGNNFNSHEECEYRCG-EVQDLCSLPKVIGPC---DGYVKQFYYDRRTDSCYEFEYGGCQGNK---NRFQDRESCEIKCRRQEPPAPSTTAQIVTTPTPSVEEPKSPICLAPADPGNCDGTITSYYYDAQQRMCQAFIYGGCGGNANKFQTEEQCERLC---------GKFHGQDVCNLPMDFGPCRGAFRKYYYESSTRTCREFTYGGCDGNANRFSTIPECESICIHHE 2365 HSP 4 Score: 162.54 bits (410), Expect = 1.796e-37 Identity = 108/333 (32.43%), Postives = 155/333 (46.55%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD-----CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEP-----MFALEGCLLRPERGSC-HGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
C L GPC +++FY+ C F++GGC+ NGNNF S EECE C E + D C P + G C G ++F++D ++ +C +F Y GC GN N F C+ C+ +P S I P S+ + CL P G+C Y ++ + C +IY GCGG+ N F + E C+ C + C + F PC + + Y R C GC N FST ECE C+ H V P + L C + GSC G RFY++ + C +F ++GC GN NNF++F+ C+ TC
Sbjct: 2115 CFLRDEHGPCTEDQVKWFYDSSVGVCRQFLYGGCQSNGNNFNSHEECEYRCGEVQ----DLCSLPKVIGPCDGYVKQFYYDRRTDSCYEFEYGGCQGNKNRFQDRESCEIKCR-RQEPPAPSTTAQIVTTPTPSVEEPKSPICLAPADPGNCDGTITSYYYDAQQRMCQAFIYGGCGGNANKFQTEEQCERLCGKFHGQDVCNLPMDFGPCRGA---FRKYYYESSTRTCREFTYGGCDGN---ANRFSTIPECESICIH---HEEPVPPGNDTSVSNLSICREPVDSGSCTSGGTKRFYFDEEHQVCRAFIYTGCGGNRNNFKTFESCIITC 2433 HSP 5 Score: 159.844 bits (403), Expect = 1.293e-36 Identity = 122/431 (28.31%), Postives = 184/431 (42.69%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC----------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC------------EIQIAPRCRIEHTFEPCLPSPS-------YTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC--HDAEV-RADGSILTNASICEQPKLKGPCRR-DQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL--NREVDA 628
C L G C Y R++Y+ ++C F +GGC GN NNF++ +C C + + + C G C+ K+ +D C +F+Y GC NGNNF+S EC+ C + D C P G C + +++ + C + Y GC G+ N F E+C+ +C +I P +E P +P+ + + Y +QRMC + GC N+F T +CER C + H +V C L + G C G+ ++YYE TC FT+ GC GN N F + C + C H+ V + + ++N SIC +P G C RF+++ + C F Y GC G+RNNF T C TCL + E+D
Sbjct: 2044 CLLPALPGECHNYTHRWYYDSYEQQCRLFYYGGCGGNENNFETEHDCVNRCVTVVTTPTPPGKQAEFRREFCFLRDEHGPCTEDQVKWFYDSSVGVCRQFLYGGCQSNGNNFNSHEECEYRCGEVQDL-------------------CSLPKVIGPCDGYVKQFYYDRRTDSCYEFEYGGCQGNKNRFQDRESCEIKCRRQEPPAPSTTAQIVTTPTPSVEEPKSPICLAPADPGNCDGTITSYYYDAQQRMCQAFIYGGCGGN---ANKFQTEEQCERLC--GKFHGQDV--------CNLPMDFGPCRGAFRKYYYESSTRTCREFTYGGCDGNANRFSTIPECESICIHHEEPVPPGNDTSVSNLSICREPVDSGSCTSGGTKRFYFDEEHQVCRAFIYTGCGGNRNNFKTFESCIITCLRTSNEIDV 2442 HSP 6 Score: 129.413 bits (324), Expect = 7.030e-27 Identity = 116/414 (28.02%), Postives = 173/414 (41.79%), Query Frame = 0
Query: 20 EFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQ-EPRRSLKNLTLDFDQGFEDWDN--YDWQI-----ISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQ--VDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQL-EQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
+ CLL G C Y R++++ C F + GCG NENNF + C C+ TT +Q E RR L + ED YD + Y + G N S + C Q S PK++ P V + T S G +N + + + + PST A TT T P S + C G CD + ++Y+ + C+ F++GGC GN N F++ E+CE C K D C P+ G C G+ K++++ + TC +F Y GC GN N F ++ EC+S C H +P + +LS C +P+ SG C + F++ C +IY+GCGG+ N F + E+C C
Sbjct: 2042 DTCLLPALPGECHNYTHRWYYDSYEQQCRLFYYGGCGGNENNFETEHDCVNRCVTVVTTPTPPGKQAEFRREFCFLRDEHGPCTEDQVKWFYDSSVGVCRQFLYGGCQSNGNNFNSHEECEYRCG---EVQDLC-SLPKVIGPCDGYVKQFYYDRRTDSCYEFEYGGCQGNKNRFQDRESCEIKCRRQEPPAPSTTAQIVTTPTPSVEEPKSPI-----------CLAPADPGNCDGTITSYYYDAQQRMCQAFIYGGCGGNANKFQTEEQCERLCG--KFHGQDVCNLPMDFGPCRGAFRKYYYESSTRTCREFTYGGCDGNANRFSTIPECESICIHHEEPVPPGNDTSV-----SNLSICREPVDSGSCTSGGTKRFYFDEEHQVCRAFIYTGCGGNRNNFKTFESCIITC 2433 HSP 7 Score: 90.5077 bits (223), Expect = 8.215e-15 Identity = 57/180 (31.67%), Postives = 79/180 (43.89%), Query Frame = 0
Query: 445 YTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
+T W Y + C GC + N F T EC+ C+ + + C L G C G P +Y++ + C F + GC GN N F++ + C C V DG CE PK GPC + R+++N + CE+F YGGCK + NNFPT C CL
Sbjct: 1819 FTVKWYYDTDYGGCSRFWYGGCKGNE---NRFKTQEECKDVCVQPKGKA----------ACFLPKIAGPCQGYHPTWYFDAERKQCGQFVYGGCLGNANKFKTKEECEELC----VTPDG-----IDPCELPKETGPCAGNFTRWYFNAESQDCEQFVYGGCKANGNNFPTEIACHQQCL 1976
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|951556761|ref|XP_014477806.1| (PREDICTED: papilin isoform X2 [Dinoponera quadriceps]) HSP 1 Score: 214.542 bits (545), Expect = 1.662e-54 Identity = 131/402 (32.59%), Postives = 194/402 (48.26%), Query Frame = 0
Query: 233 SPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSP--SYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEV--EPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDA 628
+ C L GPC Y ++++ + ++C FV+GGC GN N FK+ EECE C P+ DPC P G C+G+ +++F+ +S C +FVY GC NGNNF + C C P R C P T G C +Q + F+Q+EN+C+P+ Y+GCG ++N F S EAC++ C +I + P LP +YT W Y ++ C + GC + N F T +C C++ + + F E C LR E G C +++Y+ C F + GC NGNNF S + C C + + +C PK+ GPC +F+Y+ ++C EF YGGC+G++N F R CE C +E A
Sbjct: 1864 AACFLPKIAGPCQGYHPTWYFDAERKQCGQFVYGGCLGNANKFKTKEECEELCV--TPDGIDPCELPKETGPCAGNFTRWYFNAESQDCEQFVYGGCKANGNNFPTEIACHQQCLQ----------------PGRRRDTCTLPRTEGTCTEKQSRWYFDQSENRCMPFYYTGCGSNENNFESREACESDCPAKI----EQDTCLLPALPGECHNYTHRWYYDSYEQQCRLFYYGGCGGNE---NNFETEHDCVNRCVTVVTTPTPPGKQAEFRREFCFLRDEHGPCTEDQVKWFYDSSVGVCRQFLYGGCQSNGNNFNSHEECEYRCGEVQ-----------DLCSLPKVIGPCDGYVKQFYYDRRTDSCYEFEYGGCQGNKNRFQDRESCEIKCRRQEPPA 2229 HSP 2 Score: 176.022 bits (445), Expect = 8.437e-42 Identity = 123/415 (29.64%), Postives = 179/415 (43.13%), Query Frame = 0
Query: 234 PCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-------------CRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGS---ILTNAS---------ICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
PC+L GPC R+++N +++ CE FV+GGCK NGNNF + C C + + D C P +G+C+ +++FD + C F Y+GCG N NNF+S C+S C ++ CL P G+C + ++ E QC + Y GCGG++N F + C NRC + C + PC W Y +C + GC + GN F++ ECE C EV+ + C L G C G + +FYY+ ++C+ F + GC GN N FQ + C C E A + I+T + IC P G C ++Y+ C+ F YGGC G+ N F T QCE C
Sbjct: 1924 PCELPKETGPCAGNFTRWYFNAESQDCEQFVYGGCKANGNNFPTEIACHQQCLQPG-RRRDTCTLPRTEGTCTEKQSRWYFDQSENRCMPFYYTGCGSNENNFESREACESDCPAKIE-----------------QDTCLLPALPGECHNYTHRWYYDSYEQQCRLFYYGGCGGNENNFETEHDCVNRCVTVVTTPTPPGKQAEFRREFCFLRDEHGPCTED---QVKWFYDSSVGVCRQFLYGGCQSN---GNNFNSHEECEYRC-------GEVQDL-----CSLPKVIGPCDGYVKQFYYDRRTDSCYEFEYGGCQGNKNRFQDRESCEIKCRRQEPPAPSTTAQIVTTPTPSVEEPKSPICLAPADPGNCDGTITSYYYDAQQRMCQAFIYGGCGGNANKFQTEEQCERLC 2302 HSP 3 Score: 167.162 bits (422), Expect = 6.620e-39 Identity = 115/404 (28.47%), Postives = 171/404 (42.33%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVV--TVGCSNTKRIGNEFSTSSECERTC------LSDRSHSLEVEPMFALEG-----CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNRE 625
C L G C R++++ +C F + GC N NNF+S E CE+ C + D C+ P L G C ++++D C F Y GCGGN NNF++ +C + C + + FR R C G C +Q + ++ + C ++Y GC + N F SHE C+ RC ++ C + PC Y + Y C GC K N F CE C + + P ++E CL + G+C G++ +YY+ C +F + GC GN N FQ+ + C C +C P GPCR +++Y S TC EF YGGC G+ N F T +CE+ C++ E
Sbjct: 1985 CTLPRTEGTCTEKQSRWYFDQSENRCMPFYYTGCGSNENNFESREACESDCPAKI--EQDTCLLPALPGECHNYTHRWYYDSYEQQCRLFYYGGCGGNENNFETEHDCVNRCVTVVTTPTPPGKQAEFR---REF--CFLRDEHGPCTEDQVKWFYDSSVGVCRQFLYGGCQSNGNNFNSHEECEYRCG-EVQDLCSLPKVIGPC---DGYVKQFYYDRRTDSCYEFEYGGCQGNK---NRFQDRESCEIKCRRQEPPAPSTTAQIVTTPTPSVEEPKSPICLAPADPGNCDGTITSYYYDAQQRMCQAFIYGGCGGNANKFQTEEQCERLC---------GKFHGQDVCNLPMDFGPCRGAFRKYYYESSTRTCREFTYGGCDGNANRFSTIPECESICIHHE 2365 HSP 4 Score: 162.155 bits (409), Expect = 2.421e-37 Identity = 108/333 (32.43%), Postives = 155/333 (46.55%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSD-----CLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEP-----MFALEGCLLRPERGSC-HGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
C L GPC +++FY+ C F++GGC+ NGNNF S EECE C E + D C P + G C G ++F++D ++ +C +F Y GC GN N F C+ C+ +P S I P S+ + CL P G+C Y ++ + C +IY GCGG+ N F + E C+ C + C + F PC + + Y R C GC N FST ECE C+ H V P + L C + GSC G RFY++ + C +F ++GC GN NNF++F+ C+ TC
Sbjct: 2115 CFLRDEHGPCTEDQVKWFYDSSVGVCRQFLYGGCQSNGNNFNSHEECEYRCGEVQ----DLCSLPKVIGPCDGYVKQFYYDRRTDSCYEFEYGGCQGNKNRFQDRESCEIKCR-RQEPPAPSTTAQIVTTPTPSVEEPKSPICLAPADPGNCDGTITSYYYDAQQRMCQAFIYGGCGGNANKFQTEEQCERLCGKFHGQDVCNLPMDFGPCRGA---FRKYYYESSTRTCREFTYGGCDGN---ANRFSTIPECESICIH---HEEPVPPGNDTSVSNLSICREPVDSGSCTSGGTKRFYFDEEHQVCRAFIYTGCGGNRNNFKTFESCIITC 2433 HSP 5 Score: 159.458 bits (402), Expect = 1.991e-36 Identity = 122/431 (28.31%), Postives = 184/431 (42.69%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTC----------SEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC------------EIQIAPRCRIEHTFEPCLPSPS-------YTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC--HDAEV-RADGSILTNASICEQPKLKGPCRR-DQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL--NREVDA 628
C L G C Y R++Y+ ++C F +GGC GN NNF++ +C C + + + C G C+ K+ +D C +F+Y GC NGNNF+S EC+ C + D C P G C + +++ + C + Y GC G+ N F E+C+ +C +I P +E P +P+ + + Y +QRMC + GC N+F T +CER C + H +V C L + G C G+ ++YYE TC FT+ GC GN N F + C + C H+ V + + ++N SIC +P G C RF+++ + C F Y GC G+RNNF T C TCL + E+D
Sbjct: 2044 CLLPALPGECHNYTHRWYYDSYEQQCRLFYYGGCGGNENNFETEHDCVNRCVTVVTTPTPPGKQAEFRREFCFLRDEHGPCTEDQVKWFYDSSVGVCRQFLYGGCQSNGNNFNSHEECEYRCGEVQDL-------------------CSLPKVIGPCDGYVKQFYYDRRTDSCYEFEYGGCQGNKNRFQDRESCEIKCRRQEPPAPSTTAQIVTTPTPSVEEPKSPICLAPADPGNCDGTITSYYYDAQQRMCQAFIYGGCGGN---ANKFQTEEQCERLC--GKFHGQDV--------CNLPMDFGPCRGAFRKYYYESSTRTCREFTYGGCDGNANRFSTIPECESICIHHEEPVPPGNDTSVSNLSICREPVDSGSCTSGGTKRFYFDEEHQVCRAFIYTGCGGNRNNFKTFESCIITCLRTSNEIDV 2442 HSP 6 Score: 129.798 bits (325), Expect = 5.732e-27 Identity = 115/414 (27.78%), Postives = 174/414 (42.03%), Query Frame = 0
Query: 20 EFCLLLPERGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQ-EPRRSLKNLTLDFDQGFEDWDN--YDWQI-----ISYNSDLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQ--VDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQL-EQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC 422
+ CLL G C Y R++++ C F + GCG NENNF + C C+ TT +Q E RR L + ED YD + Y + G N S + C Q S PK++ P V + T S G +N + + + + PST A TT T P S + C G CD + ++Y+ + C+ F++GGC GN N F++ E+CE C + + D C P+ G C G+ K++++ + TC +F Y GC GN N F ++ EC+S C H +P + +LS C +P+ SG C + F++ C +IY+GCGG+ N F + E+C C
Sbjct: 2042 DTCLLPALPGECHNYTHRWYYDSYEQQCRLFYYGGCGGNENNFETEHDCVNRCVTVVTTPTPPGKQAEFRREFCFLRDEHGPCTEDQVKWFYDSSVGVCRQFLYGGCQSNGNNFNSHEECEYRCG---EVQDLC-SLPKVIGPCDGYVKQFYYDRRTDSCYEFEYGGCQGNKNRFQDRESCEIKCRRQEPPAPSTTAQIVTTPTPSVEEPKSPI-----------CLAPADPGNCDGTITSYYYDAQQRMCQAFIYGGCGGNANKFQTEEQCERLCGKFHGQ--DVCNLPMDFGPCRGAFRKYYYESSTRTCREFTYGGCDGNANRFSTIPECESICIHHEEPVPPGNDTSV-----SNLSICREPVDSGSCTSGGTKRFYFDEEHQVCRAFIYTGCGGNRNNFKTFESCIITC 2433 HSP 7 Score: 90.5077 bits (223), Expect = 8.948e-15 Identity = 57/180 (31.67%), Postives = 79/180 (43.89%), Query Frame = 0
Query: 445 YTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
+T W Y + C GC + N F T EC+ C+ + + C L G C G P +Y++ + C F + GC GN N F++ + C C V DG CE PK GPC + R+++N + CE+F YGGCK + NNFPT C CL
Sbjct: 1819 FTVKWYYDTDYGGCSRFWYGGCKGNE---NRFKTQEECKDVCVQPKGKA----------ACFLPKIAGPCQGYHPTWYFDAERKQCGQFVYGGCLGNANKFKTKEECEELC----VTPDG-----IDPCELPKETGPCAGNFTRWYFNAESQDCEQFVYGGCKANGNNFPTEIACHQQCL 1976
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|674580026|emb|CDS35863.1| (Papilin [Echinococcus multilocularis]) HSP 1 Score: 207.608 bits (527), Expect = 2.053e-54 Identity = 131/413 (31.72%), Postives = 194/413 (46.97%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKP---------EKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPR-------CRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTV--GCSNTKRIGNEFSTSSECERTC------LSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC--HDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
C L M GPC L+R+ Y+ K ++C F FGGC GN NNF++LEECE C P D C P G C G ++ FD + TC +F+Y GC GN NNF S C + C ++ + V + ++ C P G+C+ Q +G++ QCV +IY GC G+ N F + EAC+ RC ++ C++ PC S W + + C + V GC N F + CE C L S S + + C L ++G+C+ + R+ ++ C F + GC GN NNF + + C C + + R S + +C+ P+ GPCR R++Y+ + E C EF YGGC+G+ NNF ++ CEA C
Sbjct: 138 CLLEMDHGPCFALLLRYAYDKKLDQCVEFTFGGCGGNKNNFETLEECEKNCKISIPMPSTSVKFDSLDAVCELPQDTGPCKGFYRRWSFDVSTGTCVQFIYGGCKGNANNFQSKEACAAKCGVLSGVKSETVVSSVGGVGENAV--CYLPRERGNCEAYQQKWGYDPELGQCVQFIYGGCNGNANNFETKEACERRCLASLSTTLDAIDDVCKLPQDTGPCRASQRR---WAFDTSKAKCTLFVYGGCRGN---ANNFESKEACEAKCGVNKTELDASSKSGNINTLV----CHLPQDQGNCYALIRRWAFDANSSQCIPFFYGGCGGNANNFDTREACEQRCLANISMARPVTSSVALDPVCKLPQEVGPCRAMWKRWYYDVAKEQCVEFVYGGCRGNANNFESKEDCEARC 538 HSP 2 Score: 206.453 bits (524), Expect = 4.985e-54 Identity = 131/421 (31.12%), Postives = 195/421 (46.32%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCIS-------------PLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-----EIQIAPRCRIEHTFEPCLPSP-----SYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEG-CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNA--SICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVD 627
C+L GPC + R+ ++ T C F++GGCKGN NNF+S E C C K + +S P +G+C +K+ +D + C +F+Y GC GN NNF++ C+ C + T++++ C P +G C+ Q + F+ ++ +C ++Y GC G+ N F S EAC+ +C E+ + + +T LP + W + C+ GC N F T CE+ CL++ S + V AL+ C L E G C R+YY+ E C F + GC GN NNF+S + C E R ++TN+ S+C PK GPCR RFF++ C EF YGGC G+ NNF T +C +TC N D
Sbjct: 208 CELPQDTGPCKGFYRRWSFDVSTGTCVQFIYGGCKGNANNFQSKEACAAKCGVLSGVKSETVVSSVGGVGENAVCYLPRERGNCEAYQQKWGYDPELGQCVQFIYGGCNGNANNFETKEACERRCLASLSTTLDAID-----------DVCKLPQDTGPCRASQRRWAFDTSKAKCTLFVYGGCRGNANNFESKEACEAKCGVNKTELDASSKSGNINTLVCHLPQDQGNCYALIRRWAFDANSSQCIPFFYGGCGGN---ANNFDTREACEQRCLANISMARPVTSSVALDPVCKLPQEVGPCRAMWKRWYYDVAKEQCVEFVYGGCRGNANNFESKEDC-------EARCGAGVMTNSFSSVCFLPKSTGPCRGRIKRFFFDSEAGKCAEFIYGGCMGNANNFFTMEECVSTCTNMVYD 607 HSP 3 Score: 199.904 bits (507), Expect = 1.130e-51 Identity = 143/459 (31.15%), Postives = 212/459 (46.19%), Query Frame = 0
Query: 184 SFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCS-EHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSL-SDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC--------EIQIAPRCRIEHTFEPCLPS-----PSYTSVWVYSEEQRMCV--VTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILT---NASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCL 622
+F ++ LV+ L + DS+ T T A +S S+ C L A+GPC + R++Y+ + KC+ FV+GGC GN NNF ++++C TCS P K + C+ + G C ++ +D + C +F + GCGGN NNF++L EC+ CK + S V SL + C P +G C+ + F+ + CV +IY GC G+ N F S EAC +C E ++ + LP +Y W Y E CV + GC+ N F T CER CL+ S +L+ + C L + G C S R+ ++ C F + GC GN NNF+S + C A C + D S + N +C P+ +G C R+ ++ + C F YGGC G+ NNF TR CE CL
Sbjct: 27 AFFILATLVLTGVVLWLLLAPKHADSSNTQTTGKTRALQTASGKSQPG--LCSLPPAQGPCKANIERYYYDTTSGKCKKFVYGGCSGNNNNFITIQQCMETCSLPLVPSKPENCLLEMDHGPCFALLLRYAYDKKLDQCVEFTFGGCGGNKNNFETLEECEKNCKISIPMPSTS-------VKFDSLDAVCELPQDTGPCKGFYRRWSFDVSTGTCVQFIYGGCKGNANNFQSKEACAAKCGVLSGVKSETVVSSVGGVGENAVCYLPRERGNCEAYQQKWGYDPELGQCVQFIYGGCNGN---ANNFETKEACERRCLASLSTTLDAID----DVCKLPQDTGPCRASQRRWAFDTSKAKCTLFVYGGCRGNANNFESKEACEAKCGVNKTELDASSKSGNINTLVCHLPQDQGNCYALIRRWAFDANSSQCIPFFYGGCGGNANNFDTREACEQRCL 469
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|1207982839|ref|XP_021341002.1| (papilin-like isoform X6 [Mizuhopecten yessoensis]) HSP 1 Score: 214.157 bits (544), Expect = 2.216e-54 Identity = 128/396 (32.32%), Postives = 196/396 (49.49%), Query Frame = 0
Query: 234 PCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNAS-ICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREV 626
PC+L GPC + RF++N +TE+CE F +GGC+GN NNF +++ C TC E E+ D C P+ G C S +F+++ Q+ C++F Y GC GN NNF + +C C ++ P++ VP CL +G C + ++ E C ++Y GCG ++N F S E C+N C + C + P + +Y + Y E C + GC N F+T C R C++ S + PM E C L E G C + R++++ +C F + GC GN NNF++ + C C + V G + T++S C GPCR P ++Y+ + C+EF YGGC G+ N F ++ CEA+C V
Sbjct: 951 PCKLPKDIGPCRALVPRFYFNQQTERCEQFGYGGCQGNANNFATVDLCRQTCEEIIVEELDTCQQPMEIGPCRASYPRFYYNSQTRECTQFTYGGCDGNDNNFATREKCVQHCAPNVIPSVTDAP----VVPVEEKDICLLAYETGSCSEAHSRWYYDFNEGFCKQFVYGGCGQNENNFNSKEECENSCSREHV--CGSGESNSP-IQCNAYIRRYRYDRESATCQEFIYGGCGGNS---NNFATQEACLRKCITGGSVT---RPMSQKEICALPKESGPCFAYMRRYFFDSDTGSCEQFIYGGCSGNQNNFENIETCQEKCAEDTVDGSGEVETDSSEYCGLEMDAGPCRSSVPSWYYSHTEGRCKEFLYGGCGGNNNRFNSQIVCEASCSGDAV 1333 HSP 2 Score: 197.593 bits (501), Expect = 7.145e-49 Identity = 135/453 (29.80%), Postives = 196/453 (43.27%), Query Frame = 0
Query: 215 PSTTPAS---------TLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYT---------------SVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSI---LTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESE 638
P TPAS T+ +G C RGPC Y +++F+N ++CE F +GGC+GNGN F+S EEC TC E + + PC P G C +F+F+ Q+ C +F Y GC GN NNF ++ C+ TC++ + L C +P+ G C+ + +N +C + Y GC G+DN F + E C C + P T P +P S W Y + C V GC + N F++ ECE +C R H + G C+ + R+ Y+ TC F + GC GN NNF + + CL C GS+ ++ IC PK GPC R+F++ +CE+F YGGC G++NNF C+ C VD + + SE
Sbjct: 862 PDGTPASGPNEEGCDRTVDVEIKPSSGDECNKPQMRGPCSDYTVKWFHNTDEKRCERFWYGGCQGNGNKFESEEECTRTCVEKTQDINLPCKLPKDIGPCRALVPRFYFNQQTERCEQFGYGGCQGNANNFATVDLCRQTCEE---------------IIVEELDTCQQPMEIGPCRASYPRFYYNSQTRECTQFTYGGCDGNDNNFATREKCVQHCAPNVIP----SVTDAPVVPVEEKDICLLAYETGSCSEAHSRWYYDFNEGFCKQFVYGGCGQNE---NNFNSKEECENSC--SREH---------VCGSGESNSPIQCNAYIRRYRYDRESATCQEFIYGGCGGNSNNFATQEACLRKCITG-----GSVTRPMSQKEICALPKESGPCFAYMRRYFFDSDTGSCEQFIYGGCSGNQNNFENIETCQEKCAEDTVDGSGEVETDSSE 1276 HSP 3 Score: 187.193 bits (474), Expect = 1.604e-45 Identity = 143/528 (27.08%), Postives = 225/528 (42.61%), Query Frame = 0
Query: 147 SHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMN-----------DLV---VLQTNQSLRPST-----IATDSTTTTLPSTTPA-STLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEK-DDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHM---------------DPTMNSLGRHIFRVPKRSLSD----CLKPITSGDCQLEQGVYGFN------QTENQCVPYIYSGC-GGSDNLFLSHEACKNRCEIQIAP----RCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
+ P+ + P+ VD N A+ +TL+ + + + R + N ++V + T +L+P+T I + P++ P T + G C+ RG C Y++R+ Y+ +C+ F +GGC NGNNF+S E+C C P K D C+ P + G C + ++ F+ + +C F Y GC GN N F + EC + C + D + G + P + C +T+ E+G G + T+N C ++ C G+ + E C +++I P C PC YT W ++ +++ C GC GN+F + EC RTC+ +++ + + C L + G C +PRFY+ E C F + GC GN NNF + C TC + I+ C+QP GPCR PRF+YN C +F YGGC G+ NNF TR +C C
Sbjct: 560 TAPRDLTPVSVDGN-ALAVTLNWQPPKDTNGQIERYMLYYTTNAREEEDSWNRQEMVGDHLTSTVNNLKPNTLYYFKIRASNREGMGPASKPVFYTTPNEMVAPTGLLCEREPMRGQCTDYVVRWHYDRDDARCKRFWYGGCNSNGNNFESEEKCLQNC--KTPVKVQDVCVLPSVTGRCRAAHRRYFFNSATGSCELFTYGGCNGNANRFTTREECVARCGGRVDNRRLCVVSQYGCCRDGVTPADGPDNYGCPDHCVRSEFGCCEDQVTAATGPNEEGCDGVDGGSGDMDTDNDCRENVHGCCPDGTPASGPNEEGCDRTVDVEIKPSSGDECNKPQMRGPC---SDYTVKWFHNTDEKRCERFWYGGCQGN---GNKFESEEECTRTCV-EKTQDINLP-------CKLPKDIGPCRALVPRFYFNQQTERCEQFGYGGCQGNANNFATVDLCRQTCEEI-------IVEELDTCQQPMEIGPCRASYPRFYYNSQTRECTQFTYGGCDGNDNNFATREKCVQHC 1063 HSP 4 Score: 177.563 bits (449), Expect = 2.401e-42 Identity = 127/422 (30.09%), Postives = 184/422 (43.60%), Query Frame = 0
Query: 213 TLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-----------EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
+PS T A + ++ C LA G C R++Y++ C+ FV+GGC N NNF S EECE +CS SP+ C+ ++ +D +S TC +F+Y GCGGN NNF + C C T S+ R P C P SG C Y F+ C +IY GC G+ N F + E C+ +C E + C +E PC S W YS + C + GC N F++ CE +C D C+L+P++G+C S+ R+YY+ + C S + GC GN NNF+S C+ C + V + + ++C +G C ++FYN C F YGGC G+ N F T +C+ C
Sbjct: 1067 VIPSVTDAPVVPVEEKDI----CLLAYETGSCSEAHSRWYYDFNEGFCKQFVYGGCGQNENNFNSKEECENSCSREHVCGSGESNSPI---QCNAYIRRYRYDRESATCQEFIYGGCGGNSNNFATQEACLRKCI-----TGGSVTR-----PMSQKEICALPKESGPCFAYMRRYFFDSDTGSCEQFIYGGCSGNQNNFENIETCQEKCAEDTVDGSGEVETDSSEYCGLEMDAGPCRSS---VPSWYYSHTEGRCKEFLYGGCGGNN---NRFNSQIVCEASCSGDAV-------------CMLQPDKGNCVRSVQRYYYDVSQDACVSLDYGGCGGNANNFESRAACVGKCGGSYVTPE---PHSDNVCAMDNDRGSCSNYAVQWFYNQDEGVCGRFWYGGCDGNGNRFSTEQECKQRC 1449 HSP 5 Score: 125.561 bits (314), Expect = 9.080e-26 Identity = 88/319 (27.59%), Postives = 132/319 (41.38%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
C L M GPC + ++Y+ +C+ F++GGC GN N F S CE +CS D C+ KG+C S +++++D C Y GCGGN NNF+S A C C G ++ P S + C G C + +NQ E C + Y GC G+ N F + + CK RC +++ R T+ P P+Y V T R ++ + + + + + + C L RG C ++Y+ D+ C F + GC GN NNF C C
Sbjct: 1278 CGLEMDAGPCRSSVPSWYYSHTEGRCKEFLYGGCGGNNNRFNSQIVCEASCS-----GDAVCMLQPDKGNCVRSVQRYYYDVSQDACVSLDYGGCGGNANNFESRAACVGKCG----------GSYVTPEP-HSDNVCAMDNDRGSCSNYAVQWFYNQDEGVCGRFWYGGCDGNGNRFSTEQECKQRCGVEVVTA-RPRPTYAPVTVRPTYAPV-----------------TTPRPDPVYTPRP-------AYTTPNPDTNVIVEYDICDLDQARGQCSNYTVKWYFNKDDQKCMRFWYGGCDGNANNFDDEAECQNIC 1555 HSP 6 Score: 88.9669 bits (219), Expect = 2.782e-14 Identity = 48/130 (36.92%), Postives = 66/130 (50.77%), Query Frame = 0
Query: 497 MFALEG--CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNR 624
M A G C P RG C + R++Y+ D C F + GC+ NGNNF+S + CL C + + +C P + G CR R+F+N + +CE F YGGC G+ N F TR +C A C R
Sbjct: 659 MVAPTGLLCEREPMRGQCTDYVVRWHYDRDDARCKRFWYGGCNSNGNNFESEEKCLQNCK--------TPVKVQDVCVLPSVTGRCRAAHRRYFFNSATGSCELFTYGGCNGNANRFTTREECVARCGGR 780 HSP 7 Score: 78.5666 bits (192), Expect = 3.574e-11 Identity = 51/172 (29.65%), Postives = 76/172 (44.19%), Query Frame = 0
Query: 201 RPSTIATDSTTTTLPSTTPASTLSSSTSELAGSP-------CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPE--KDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFR 363
RP T + TL +S G P C L RGPC + +++Y+ + C F +GGC GN N F+S E+C+ CS + D C +GSCS +++++ C +F Y C GNGNNF EC + C + P + S R ++
Sbjct: 235 RPDGDGTRNCQNTLYGCCQDGRTASLGPRFQGCPTDAQDSRCGLEKDRGPCSEFGAKWYYDSGRKGCFQFWYGGCDGNTNRFESEEDCQKHCSGYTGTVGGTDVCRLDSERGSCSDYTVRWYYNSDESRCERFWYGSCDGNGNNFADEQECNAACVNA--PVVTSAPREEYK 404 HSP 8 Score: 72.4034 bits (176), Expect = 3.195e-9 Identity = 40/124 (32.26%), Postives = 56/124 (45.16%), Query Frame = 0
Query: 503 CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREV 626
C L +RG C ++YY+ + C F + GC GN N F+S + C C G+ +C +G C R++YN CE F YG C G+ NNF +C A C+N V
Sbjct: 276 CGLEKDRGPCSEFGAKWYYDSGRKGCFQFWYGGCDGNTNRFESEEDCQKHCSGYTGTVGGT-----DVCRLDSERGSCSDYTVRWYYNSDESRCERFWYGSCDGNGNNFADEQECNAACVNAPV 394
BLAST of kunitz-like protease inhibitor precursor vs. nr
Match: gi|1207982843|ref|XP_021341004.1| (papilin-like isoform X8 [Mizuhopecten yessoensis]) HSP 1 Score: 214.157 bits (544), Expect = 2.294e-54 Identity = 128/396 (32.32%), Postives = 196/396 (49.49%), Query Frame = 0
Query: 234 PCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNAS-ICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREV 626
PC+L GPC + RF++N +TE+CE F +GGC+GN NNF +++ C TC E E+ D C P+ G C S +F+++ Q+ C++F Y GC GN NNF + +C C ++ P++ VP CL +G C + ++ E C ++Y GCG ++N F S E C+N C + C + P + +Y + Y E C + GC N F+T C R C++ S + PM E C L E G C + R++++ +C F + GC GN NNF++ + C C + V G + T++S C GPCR P ++Y+ + C+EF YGGC G+ N F ++ CEA+C V
Sbjct: 834 PCKLPKDIGPCRALVPRFYFNQQTERCEQFGYGGCQGNANNFATVDLCRQTCEEIIVEELDTCQQPMEIGPCRASYPRFYYNSQTRECTQFTYGGCDGNDNNFATREKCVQHCAPNVIPSVTDAP----VVPVEEKDICLLAYETGSCSEAHSRWYYDFNEGFCKQFVYGGCGQNENNFNSKEECENSCSREHV--CGSGESNSP-IQCNAYIRRYRYDRESATCQEFIYGGCGGNS---NNFATQEACLRKCITGGSVT---RPMSQKEICALPKESGPCFAYMRRYFFDSDTGSCEQFIYGGCSGNQNNFENIETCQEKCAEDTVDGSGEVETDSSEYCGLEMDAGPCRSSVPSWYYSHTEGRCKEFLYGGCGGNNNRFNSQIVCEASCSGDAV 1216 HSP 2 Score: 197.208 bits (500), Expect = 7.742e-49 Identity = 135/454 (29.74%), Postives = 197/454 (43.39%), Query Frame = 0
Query: 215 PSTTPAS---------TLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYT---------------SVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSL-EVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSI---LTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNREVDAFIQFLAEESE 638
P TPAS T+ +G C RGPC Y +++F+N ++CE F +GGC+GNGN F+S EEC TC E + + PC P G C +F+F+ Q+ C +F Y GC GN NNF ++ C+ TC++ + L C +P+ G C+ + +N +C + Y GC G+DN F + E C C + P T P +P S W Y + C V GC + N F++ ECE +C R H E ++ C+ + R+ Y+ TC F + GC GN NNF + + CL C GS+ ++ IC PK GPC R+F++ +CE+F YGGC G++NNF C+ C VD + + SE
Sbjct: 745 PDGTPASGPNEEGCDRTVDVEIKPSSGDECNKPQMRGPCSDYTVKWFHNTDEKRCERFWYGGCQGNGNKFESEEECTRTCVEKTQDINLPCKLPKDIGPCRALVPRFYFNQQTERCEQFGYGGCQGNANNFATVDLCRQTCEE---------------IIVEELDTCQQPMEIGPCRASYPRFYYNSQTRECTQFTYGGCDGNDNNFATREKCVQHCAPNVIP----SVTDAPVVPVEEKDICLLAYETGSCSEAHSRWYYDFNEGFCKQFVYGGCGQNE---NNFNSKEECENSC--SREHVCGSGESNSPIQ----------CNAYIRRYRYDRESATCQEFIYGGCGGNSNNFATQEACLRKCITG-----GSVTRPMSQKEICALPKESGPCFAYMRRYFFDSDTGSCEQFIYGGCSGNQNNFENIETCQEKCAEDTVDGSGEVETDSSE 1159 HSP 3 Score: 187.193 bits (474), Expect = 1.436e-45 Identity = 143/528 (27.08%), Postives = 225/528 (42.61%), Query Frame = 0
Query: 147 SHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMN-----------DLV---VLQTNQSLRPST-----IATDSTTTTLPSTTPA-STLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEK-DDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHM---------------DPTMNSLGRHIFRVPKRSLSD----CLKPITSGDCQLEQGVYGFN------QTENQCVPYIYSGC-GGSDNLFLSHEACKNRCEIQIAP----RCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
+ P+ + P+ VD N A+ +TL+ + + + R + N ++V + T +L+P+T I + P++ P T + G C+ RG C Y++R+ Y+ +C+ F +GGC NGNNF+S E+C C P K D C+ P + G C + ++ F+ + +C F Y GC GN N F + EC + C + D + G + P + C +T+ E+G G + T+N C ++ C G+ + E C +++I P C PC YT W ++ +++ C GC GN+F + EC RTC+ +++ + + C L + G C +PRFY+ E C F + GC GN NNF + C TC + I+ C+QP GPCR PRF+YN C +F YGGC G+ NNF TR +C C
Sbjct: 443 TAPRDLTPVSVDGN-ALAVTLNWQPPKDTNGQIERYMLYYTTNAREEEDSWNRQEMVGDHLTSTVNNLKPNTLYYFKIRASNREGMGPASKPVFYTTPNEMVAPTGLLCEREPMRGQCTDYVVRWHYDRDDARCKRFWYGGCNSNGNNFESEEKCLQNC--KTPVKVQDVCVLPSVTGRCRAAHRRYFFNSATGSCELFTYGGCNGNANRFTTREECVARCGGRVDNRRLCVVSQYGCCRDGVTPADGPDNYGCPDHCVRSEFGCCEDQVTAATGPNEEGCDGVDGGSGDMDTDNDCRENVHGCCPDGTPASGPNEEGCDRTVDVEIKPSSGDECNKPQMRGPC---SDYTVKWFHNTDEKRCERFWYGGCQGN---GNKFESEEECTRTCV-EKTQDINLP-------CKLPKDIGPCRALVPRFYFNQQTERCEQFGYGGCQGNANNFATVDLCRQTCEEI-------IVEELDTCQQPMEIGPCRASYPRFYYNSQTRECTQFTYGGCDGNDNNFATREKCVQHC 946 HSP 4 Score: 184.882 bits (468), Expect = 8.512e-45 Identity = 169/618 (27.35%), Postives = 247/618 (39.97%), Query Frame = 0
Query: 28 RGHCFFYERRYFFNQSSGACETFSFSGCGPNENNFRYKKACERMCMNGNTTEEEILRQEPR-----RSLKNLTLDFDQGFEDWDNYDWQIISYNS------DLARGLNLYSPDPGSRSCAGPLRPQGFSKSHPKLMHPIQVDRNEAITITLSLKLLRIESWPGRRNNSFIVMNDLVVLQTNQSLRPSTIATDSTTTTLPSTTPASTLSSSTSELAGSPCQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRC-----------EIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMC--VVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATC 621
RG C Y ++F N CE F + GC N N F ++ C R C+ T + + + P+ R+L F+Q E + + + N+ DL R +C P+ S+P+ + Q T G NN F V Q P+ I PS T A + ++ C LA G C R++Y++ C+ FV+GGC N NNF S EECE +CS SP+ C+ ++ +D +S TC +F+Y GCGGN NNF + C C T S+ R P C P SG C Y F+ C +IY GC G+ N F + E C+ +C E + C +E PC S W YS + C + GC N F++ CE +C D C+L+P++G+C S+ R+YY+ + C S + GC GN NNF+S C+ C + V + + ++C +G C ++FYN C F YGGC G+ N F T +C+ C
Sbjct: 780 RGPCSDYTVKWFHNTDEKRCERFWYGGCQGNGNKFESEEECTRTCVE-KTQDINLPCKLPKDIGPCRALV-PRFYFNQQTERCEQFGYGGCQGNANNFATVDLCRQTCEEIIVEELDTCQQPMEIGPCRASYPRFYYNSQ---------TRECTQFTYGGCDGNDNN-FATREKCV-----QHCAPNVI---------PSVTDAPVVPVEEKDI----CLLAYETGSCSEAHSRWYYDFNEGFCKQFVYGGCGQNENNFNSKEECENSCSREHVCGSGESNSPI---QCNAYIRRYRYDRESATCQEFIYGGCGGNSNNFATQEACLRKCI-----TGGSVTR-----PMSQKEICALPKESGPCFAYMRRYFFDSDTGSCEQFIYGGCSGNQNNFENIETCQEKCAEDTVDGSGEVETDSSEYCGLEMDAGPCRSS---VPSWYYSHTEGRCKEFLYGGCGGNN---NRFNSQIVCEASCSGDAV-------------CMLQPDKGNCVRSVQRYYYDVSQDACVSLDYGGCGGNANNFESRAACVGKCGGSYVTPE---PHSDNVCAMDNDRGSCSNYAVQWFYNQDEGVCGRFWYGGCDGNGNRFSTEQECKQRC 1332 HSP 5 Score: 125.561 bits (314), Expect = 9.193e-26 Identity = 88/319 (27.59%), Postives = 132/319 (41.38%), Query Frame = 0
Query: 235 CQLAMARGPCDLYLIRFFYNWKTEKCENFVFGGCKGNGNNFKSLEECETTCSEHKPEKDDPCISPLLKGSCSGSGEKFHFDFQSHTCSKFVYSGCGGNGNNFDSLAECQSTCKDHMDPTMNSLGRHIFRVPKRSLSDCLKPITSGDCQLEQGVYGFNQTENQCVPYIYSGCGGSDNLFLSHEACKNRCEIQIAPRCRIEHTFEPCLPSPSYTSVWVYSEEQRMCVVTVGCSNTKRIGNEFSTSSECERTCLSDRSHSLEVEPMFALEGCLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATC 553
C L M GPC + ++Y+ +C+ F++GGC GN N F S CE +CS D C+ KG+C S +++++D C Y GCGGN NNF+S A C C G ++ P S + C G C + +NQ E C + Y GC G+ N F + + CK RC +++ R T+ P P+Y V T R ++ + + + + + + C L RG C ++Y+ D+ C F + GC GN NNF C C
Sbjct: 1161 CGLEMDAGPCRSSVPSWYYSHTEGRCKEFLYGGCGGNNNRFNSQIVCEASCS-----GDAVCMLQPDKGNCVRSVQRYYYDVSQDACVSLDYGGCGGNANNFESRAACVGKCG----------GSYVTPEP-HSDNVCAMDNDRGSCSNYAVQWFYNQDEGVCGRFWYGGCDGNGNRFSTEQECKQRCGVEVVTA-RPRPTYAPVTVRPTYAPV-----------------TTPRPDPVYTPRP-------AYTTPNPDTNVIVEYDICDLDQARGQCSNYTVKWYFNKDDQKCMRFWYGGCDGNANNFDDEAECQNIC 1438 HSP 6 Score: 88.1965 bits (217), Expect = 3.729e-14 Identity = 48/130 (36.92%), Postives = 66/130 (50.77%), Query Frame = 0
Query: 497 MFALEG--CLLRPERGSCHGSLPRFYYEPMDETCHSFTFSGCHGNGNNFQSFQHCLATCHDAEVRADGSILTNASICEQPKLKGPCRRDQPRFFYNPSIETCEEFRYGGCKGSRNNFPTRAQCEATCLNR 624
M A G C P RG C + R++Y+ D C F + GC+ NGNNF+S + CL C + + +C P + G CR R+F+N + +CE F YGGC G+ N F TR +C A C R
Sbjct: 542 MVAPTGLLCEREPMRGQCTDYVVRWHYDRDDARCKRFWYGGCNSNGNNFESEEKCLQNCK--------TPVKVQDVCVLPSVTGRCRAAHRRYFFNSATGSCELFTYGGCNGNANRFTTREECVARCGGR 663 The following BLAST results are available for this feature:
BLAST of kunitz-like protease inhibitor precursor vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 14
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BLAST of kunitz-like protease inhibitor precursor vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of kunitz-like protease inhibitor precursor vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold580_size130538:108918..111884+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold580_size130538-snap-gene-0.29 ID=maker-scaffold580_size130538-snap-gene-0.29|Name=kunitz-like protease inhibitor precursor|organism=Tigriopus kingsejongensis|type=gene|length=2967bp|location=Sequence derived from alignment at scaffold580_size130538:108918..111884+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'kunitz-like protease inhibitor precursor' has the following synonyms
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