BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 602.438 bits (1552), Expect = 0.000e+0
Identity = 305/552 (55.25%), Postives = 404/552 (73.19%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPEN--YFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKA 550
            +  +++  ++ + +PLTLSEK+LY+HL D   ++  +RG+ Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLIQA+     DL  A   +AEV++FL +   KYG+GFWKPG+GIIHQ++LENYAFPG +M+GTDSHT N GGLG + IGVGGADAVDVMA + WELK PK+IGV+LTG +SGWT+PKDVILKVA ILTVKGGTGAIVEYFG G  ++SCTG GTICNMGAEIGATTS F Y+  M+ YL +T+R+D+A+ A++ +E       V  +P+    +DQV++IDL+ L P +NGPFTPDL+T +G ++  KA +N WP++V+ GLIGSCTNSSYED++R ++I +QAL KG+K K+   I PGSEQ+R T ERDG+     + G  +  NACGPCIGQW R +  K   KN+IV S+NRNF  R D NP TH FV SPE+T A+++AG LDF+P +D++   +G E++ + P G ELP +GF+  +  +  P ADGS V V V  DS RLQLL PF      + K +++LIKA
Sbjct:  631 LVQKLEIVKDRLNRPLTLSEKVLYSHL-DDPKNQDIVRGESYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIQAQIEGKKDLARAKDLNAEVYNFLSTSGAKYGVGFWKPGSGIIHQIILENYAFPGLLMVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNRSDIANLADKYRE-------VLLSPDGGCKYDQVVEIDLNTLEPHVNGPFTPDLATPIG-ELGEKAKKNGWPLEVKDGLIGSCTNSSYEDMARXTAIIKQALAKGMKSKSNFYITPGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWDRQNITK-GEKNTIVTSYNRNFTGRNDANPATHGFVTSPEMTVALSLAGSLDFDPRSDAIKAPDGSELKLNAPFGDELPSQGFDAGEDTYQHPPADGSSVSVTVDSDSNRLQLLTPFDKWDGKDHKEMRVLIKA 1172          

HSP 2 Score: 73.1738 bits (178), Expect = 2.706e-13
Identity = 38/89 (42.70%), Postives = 54/89 (60.67%), Query Frame = 0

Query:  641 SFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKA 729
            S A  LDF+P +D++   +G E++ + P G ELP +GF+  +  +  P ADGS V V V  DS RLQLL PF     +  K +++LIKA
Sbjct: 1084 SLAGSLDFDPRSDAIKAPDGSELKLNAPFGDELPSQGFDAGEDTYQHPPADGSSVSVTVDSDSNRLQLLTPFDKWDGKDHKEMRVLIKA 1172          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 146.747 bits (369), Expect = 3.003e-36
Identity = 160/517 (30.95%), Postives = 228/517 (44.10%), Query Frame = 0

Query:   28 WDGTPSKAFLRGKDY-VDFAPDRIACQDATAQMALLQF--MQAGKKKV---------AVPTTVHCDHLIQ---AKNGAA----ADLKFANSTSAEVFDFLE-SVSNKYGIGFWKPGAGIIHQVVLENYA----------FPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPEN--YFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMN----------------------------VKATENEWPI----QVEWGLIGSCTNSSYEDLSR-ASSIAQQALDKGLKMKAEL--GINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKN------SIVHSFNRNFAKRADGNPNTHA-FVASPEITAAIAIAGRLDFN 470
            W+G       RGK+  + F P R+  QD T   A++ F  M+   KK+           PT +  DH IQ   A+   A     DL+F  +   E F FL+   S+   +    PG+GI+HQV LE  A          FP  + +GTDSHT    GLG+V  GVGG +A  VM G    +  P+++G R++G+        D++L + + L   G  G  VE++GPG   LS   + TI NM  E GAT   F  D     YLR T+R        +   HLT     Y +      F Q++ +DL  +    +GP  P    AV TDM                             + A +N+  I     V    I SCTN+S   +   A  +A++A+DKGL +   +   ++PGS  V +  +  G++   + LG  I       CIG      D      N      + V S NRNF  R   NP+T A ++ASP +  A AIAGR+D +
Sbjct:   65 WEGN------RGKNAEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRN--KERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTDLVFPDSV-VGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAV-TDMKNDFLQCLSNKIGFKGFAIPDNKKNITVPIIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRI--NPHTRANYLASPPLVIAYAIAGRVDID 569          

HSP 2 Score: 56.6102 bits (135), Expect = 2.749e-8
Identity = 38/120 (31.67%), Postives = 61/120 (50.83%), Query Frame = 0

Query:  766 LTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTF-DNESDYDL-IQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            ++G+   V D    YK      IV+   +YG GSSR+ AA  P  LGV AV+ +S+ RIH +NL   G++ L + + ES   L     + F+     +  P + L +++   DG+  + K
Sbjct:  746 ISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDGSIKSFK 865          

BLAST of aconitate hydratase vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 131.339 bits (329), Expect = 1.841e-31
Identity = 138/446 (30.94%), Postives = 199/446 (44.62%), Query Frame = 0

Query:   92 ADLKFANSTS-----AEVFDFLESVSNKY-GIGFWKPGAGIIHQVVLENYA----FPGGMM-----IGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDR-----ADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGP-FTPDLSTA--VGTDMNVKATENEWP-----------------IQVEWGLIG----------SCTNSSYEDLSR-ASSIAQQALDKGLKMK--AELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSF-------------NRNFAKRADGNPNTHA-FVASPEITAAIAIAGRLDFN 470
            AD   +N TS      E F FL+ V++ +  I    PG G++HQV LE  A      GG++     +GTD+HT    GLG++  GVG  DA  VM G    L FP+++G+R+ G IS ++   D++L + + L   G  GA VE+FGP    LS   + TI NM  E GA    F  D    +YL  + R       V      VK   ++D     N    F  +I+IDLS + P ++GP  T D  +   +  D        E P                 I  E+  +G          SCTN+S   +   A  +A++A++ GL +    +  + PGS  V    +  G++     LG +I    C  C      Y+   N P +SIV +              NRNF  R D  P+  A ++ASP +  A AIAGR+D N
Sbjct:  158 ADALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVVTCQGGVLFPDSVVGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRSSDE----NENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYGLSQGQGHVAFSVNIDGEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTC-----SYNSGPNLP-DSIVQAIKQGNLVCCGLLSGNRNFEGRLD--PDIRANYLASPLMVIAYAIAGRIDIN 591          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|6686275|sp|Q99798.2|ACON_HUMAN (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 728.013 bits (1878), Expect = 0.000e+0
Identity = 371/660 (56.21%), Postives = 467/660 (70.76%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFN 649
            +   ++  R+ + +PLTLSEKI+Y HL D   S+   RGK Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLI+A+ G   DL+ A   + EV++FL +   KYG+GFWKPG+GIIHQ++LENYA+PG ++IGTDSHT N GGLG + IGVGGADAVDVMAG+ WELK PK+IGV+LTG++SGW++PKDVILKVA ILTVKGGTGAIVEY GPG  ++SCTG  TICNMGAEIGATTS F Y+  M++YL  T R D+A+ A+E K+HL  D      P  ++DQ+I+I+LS+L P +NGPFTPDL+  V     V   E  WP+ +  GLIGSCTNSSYED+ R++++A+QAL  GLK K++  I PGSEQ+R T ERDG   +   LG  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP THAFV SPEI  A+AIAG L FNP TD L   +G++ R + P   ELP   F+     +  P  D SG  V V+  S+RLQLLEPF      +L+ +++LIK           A GPWL+FRGHLDNI+NN LIGA+N  N K N V+N +T EF  VPDT R YK  GI+ +V+GD NYGEGSSREHAA++PRHLG  A++ KSFAR  + N
Sbjct:   48 LEKNINIVRKRLNRPLTLSEKIVYGHL-DDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPSTIHCDHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWKPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWSSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGREDIANLADEFKDHLVPD------PGCHYDQLIEINLSELKPHINGPFTPDLAHPVAEVGKVAEKEG-WPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDLGGIVLANACGPCIGQWDR-KDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDYLTGTDGKKFRLEAPDADELPKGEFDPGQDTYQHPPKDSSGQHVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETN 698          

HSP 2 Score: 242.662 bits (618), Expect = 2.796e-67
Identity = 127/257 (49.42%), Postives = 161/257 (62.65%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTI 882
            V   + A  L FNP TD L   +G++ R + P   ELP   F+     +  P  D SG  V V+  S+RLQLLEPF     + L+ +++LIK           A GPWL+FRGHLDNI+NN LIGA+N  N K N V+N +T EF  VPDT R YK  GI+ +V+GD NYGEGSSREHAA++PRHLG  A++ KSFARIHETNLKKQG+L LTF + +DY+ I   D      +  F P KPL   I H +GT++TI
Sbjct:  496 VTALAIAGTLKFNPETDYLTGTDGKKFRLEAPDADELPKGEFDPGQDTYQHPPKDSSGQHVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIQGLKDFTPGKPLKCIIKHPNGTQETI 752          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|60391194|sp|Q9ER34.2|ACON_RAT (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 727.628 bits (1877), Expect = 0.000e+0
Identity = 370/660 (56.06%), Postives = 470/660 (71.21%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFN 649
            +   ++  R+ + +PLTLSEKI+Y HL D   ++   RGK Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLI+A+ G   DL+ A   + EV++FL +   KYG+GFW+PG+GIIHQ++LENYA+PG ++IGTDSHT N GGLG + IGVGGADAVDVMAG+ WELK PK+IGV+LTGT+SGWT+PKDVILKVA ILTVKGGTGAIVEY GPG  ++SCTG  TICNMGAEIGATTS F Y+  M++YL  T RAD+A+ A E K+HL  D      P   +DQVI+I+L++L P +NGPFTPDL+  V  D+   A +  WP+ +  GLIGSCTNSSYED+ R++++A+QAL  GLK K++  I PGSEQ+R T ERDG   +   +G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP THAFV SPEI  A+AIAG L FNP TD L  ++G++ + + P   ELP   F+     +  P  D SG +V V+  S+RLQLLEPF      +L+ +++LIK           A GPWL+FRGHLDNI+NN LIGA+N  N K N V+N +T EF  VPDT R YK  GI+ +V+GD NYGEGSSREHAA++PRHLG  A++ KSFAR  + N
Sbjct:   48 LEKNINIVRKRLNRPLTLSEKIVYGHL-DDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGTLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLAEEFKDHLVPD------PGCQYDQVIEINLNELKPHINGPFTPDLAHPVA-DVGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDR-KDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRSDFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETN 698          

HSP 2 Score: 245.358 bits (625), Expect = 3.759e-68
Identity = 128/257 (49.81%), Postives = 164/257 (63.81%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTI 882
            V   + A  L FNP TD L  ++G++ + + P   ELP   F+     +  P  D SG +V V+  S+RLQLLEPF     + L+ +++LIK           A GPWL+FRGHLDNI+NN LIGA+N  N K N V+N +T EF  VPDT R YK  GI+ +V+GD NYGEGSSREHAA++PRHLG  A++ KSFARIHETNLKKQG+L LTF + SDY+ I   D      +  FAP KPL   I H +GT++TI
Sbjct:  496 VTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRSDFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLNCIIKHPNGTQETI 752          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|60391212|sp|Q99KI0.1|ACON_MOUSE (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 725.317 bits (1871), Expect = 0.000e+0
Identity = 368/660 (55.76%), Postives = 469/660 (71.06%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFN 649
            +   ++  R+ + +PLTLSEKI+Y HL D   ++   RGK Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLI+A+ G   DL+ A   + EV++FL +   KYG+GFW+PG+GIIHQ++LENYA+PG ++IGTDSHT N GGLG + IGVGGADAVDVMAG+ WELK PK+IGV+LTG++SGWT+PKDVILKVA ILTVKGGTGAIVEY GPG  ++SCTG  TICNMGAEIGATTS F Y+  M++YL  T R D+A+ A E K+HL  D      P   +DQVI+I+L++L P +NGPFTPDL+  V  D+   A +  WP+ +  GLIGSCTNSSYED+ R++++A+QAL  GLK K++  I PGSEQ+R T ERDG   +   +G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP THAFV SPEI  A+AIAG L FNP TD L  ++G++ + + P   ELP   F+     +  P  D SG +V V+  S+RLQLLEPF      +L+ +++LIK           A GPWL+FRGHLDNI+NN LIGA+N  N K N V+N +T EF  VPDT R YK  GI+ +V+GD NYGEGSSREHAA++PRHLG  A++ KSFAR  + N
Sbjct:   48 LEKNINIVRKRLNRPLTLSEKIVYGHL-DDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRTDIANLAEEFKDHLVPD------PGCQYDQVIEINLNELKPHINGPFTPDLAHPVA-DVGTVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDR-KDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRSDFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETN 698          

HSP 2 Score: 245.358 bits (625), Expect = 3.275e-68
Identity = 128/257 (49.81%), Postives = 164/257 (63.81%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTI 882
            V   + A  L FNP TD L  ++G++ + + P   ELP   F+     +  P  D SG +V V+  S+RLQLLEPF     + L+ +++LIK           A GPWL+FRGHLDNI+NN LIGA+N  N K N V+N +T EF  VPDT R YK  GI+ +V+GD NYGEGSSREHAA++PRHLG  A++ KSFARIHETNLKKQG+L LTF + SDY+ I   D      +  FAP KPL   I H +GT++TI
Sbjct:  496 VTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRSDFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPSDYNKIHPVDKLTIQGLKDFAPGKPLKCVIKHPNGTQETI 752          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|110282935|sp|P20004.4|ACON_BOVIN (RecName: Full=Aconitate hydratase, mitochondrial; Short=Aconitase; AltName: Full=Citrate hydro-lyase; Flags: Precursor)

HSP 1 Score: 724.931 bits (1870), Expect = 0.000e+0
Identity = 368/660 (55.76%), Postives = 471/660 (71.36%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFN 649
            +   ++  R+ + +PLTLSEKI+Y HL D   ++   RGK Y+   PDR+A QDATAQMA+LQF+ +G  KVAVP+T+HCDHLI+A+ G   DL+ A   + EV++FL +   KYG+GFW+PG+GIIHQ++LENYA+PG ++IGTDSHT N GGLG + IGVGGADAVDVMAG+ WELK PK+IGV+LTG++SGWT+PKDVILKVA ILTVKGGTGAIVEY GPG  ++SCTG  TICNMGAEIGATTS F Y+  M++YL  T RAD+A+ A+E K+HL  D+        ++DQ+I+I+LS+L P +NGPFTPDL+  V    +V   E  WP+ +  GLIGSCTNSSYED+ R++++A+QAL  GL+ K++  I PGSEQ+R T ERDG   +   +G  +  NACGPCIGQW R  D K   KN+IV S+NRNF  R D NP THAFV SPEI  A+AIAG L FNP TD L  ++G++ + + P   ELP   F+     +  P  D SG QV V+  S+RLQLLEPF      +L+ +++LIK           A GPWL+FRGHLDNI+NN LIGA+N  N K N V+N +T EF  VPDT R YK  GI+ +V+GD NYGEGSSREHAA++PRHLG  A++ KSFAR  + N
Sbjct:   48 LEKNINIVRKRLNRPLTLSEKIVYGHL-DDPANQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDHLIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVPDSGC------HYDQLIEINLSELKPHINGPFTPDLAHPVAEVGSVAEKEG-WPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLQCKSQFTITPGSEQIRATIERDGYAQILRDVGGIVLANACGPCIGQWDR-KDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRAEFDPGQDTYQHPPKDSSGQQVDVSPTSQRLQLLEPFDKWDGRDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINVENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETN 698          

HSP 2 Score: 247.284 bits (630), Expect = 8.002e-69
Identity = 129/257 (50.19%), Postives = 164/257 (63.81%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTI 882
            V   + A  L FNP TD L  ++G++ + + P   ELP   F+     +  P  D SG QV V+  S+RLQLLEPF       L+ +++LIK           A GPWL+FRGHLDNI+NN LIGA+N  N K N V+N +T EF  VPDT R YK  GI+ +V+GD NYGEGSSREHAA++PRHLG  A++ KSFARIHETNLKKQG+L LTF + +DY+ I   D      +  FAP KPLT  I H +GT++TI
Sbjct:  496 VTALAIAGTLKFNPETDFLTGKDGKKFKLEAPDADELPRAEFDPGQDTYQHPPKDSSGQQVDVSPTSQRLQLLEPFDKWDGRDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINVENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIKGLKDFAPGKPLTCIIKHPNGTQETI 752          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|395398603|sp|Q9P7D4.3|ACON2_SCHPO (RecName: Full=Homocitrate dehydratase, mitochondrial; AltName: Full=Aconitase 2; Flags: Precursor)

HSP 1 Score: 659.062 bits (1699), Expect = 0.000e+0
Identity = 345/692 (49.86%), Postives = 452/692 (65.32%), Query Frame = 0

Query:    1 MANRVDKAREIV-GKPLTLSEKILYAHLWDGTPSKAF-------LRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEV-KEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGV---QVAVAEDSERLQLLEPFTPIQDAELKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFN-------PLTDSLINENGQE 662
            +  ++ + R+ + G+ LTL+EK+LY+HL +  P ++F       +RG  Y+   PDR+A QDA+AQMALLQFM  G +K  +P ++HCDHLI    GA +D+  + + + E+FDFL+S + KYGI FW PG+GIIHQ+VLENYA PGGMM+GTDSHT NAGGLGM+AIGVGGADAVD M    WELK PK+IGV LTG +SGWT PKD+IL +A  LTV+GGTG I+EYFGPG  +LSCTG  T+CNMGAE+GATTS F Y  SM RYL AT RA+VADAA+EV  E+    AD  A     +DQ+IDI+LS+L P LNGPFTPDLST V +       +N+WP ++  GLIGSCTNSSY+D++    + +QA+  GLK K    + PGSEQ+R T ERDGI    E+ G+ +  NACGPCIG W R  D  +   N+I+ SFNRNF  R DGNP+T  F+ SP I AA   +  L F+P  D+L   +G+  +F  P G ELP  GF   D+ ++ PE +   V   +V +   S+RL+ LEPF P Q  E++ +K+ +K           A G WL+++GHL NI NNTLIGA+NA   + N   +   G+   +P+    +K  G   +VV +HNYGEGS+REHAA+QPR +    +L KSFAR  + N       PLT   +NE   E
Sbjct:   47 LMGKLQQVRKFLPGQKLTLAEKVLYSHLVN--PEESFSGVSPSDIRGSLYLKLNPDRVAMQDASAQMALLQFMTCGLEKTMIPASIHCDHLIVGHRGANSDIPDSIANNKEIFDFLQSAAKKYGIQFWGPGSGIIHQIVLENYAAPGGMMLGTDSHTPNAGGLGMIAIGVGGADAVDAMTNTPWELKAPKIIGVNLTGAMSGWTTPKDLILHLAGKLTVRGGTGHIIEYFGPGVASLSCTGMATVCNMGAEVGATTSIFPYTDSMRRYLIATHRAEVADAASEVHSEYNYLAADTGAK----YDQIIDINLSELTPSLNGPFTPDLSTPV-SKFGEAIEKNKWPKKLSAGLIGSCTNSSYQDMTCVVDVVEQAISAGLKPKVPFLVTPGSEQIRATIERDGITERLEEAGATVLANACGPCIGMWKRTDDIASGEPNAILTSFNRNFRSRNDGNPSTMNFLTSPVIVAAKIFSSDLAFDPTHDTLQTPDGKAFKFRPPQGVELPSAGFIAGDSSYI-PEPNPQPVPETEVTIDPKSDRLEALEPFEPYQGGEMENLKVAVKVKGKCTTDHISAAGKWLKYKGHLSNICNNTLIGAMNAATGEVNRAYDN--GKGMTIPELMWKWKKDGQPWLVVAEHNYGEGSAREHAALQPRAMNGRIILTKSFARIHETNLKKQGVLPLT--FVNEADYE 726          

HSP 2 Score: 186.422 bits (472), Expect = 1.844e-47
Identity = 106/259 (40.93%), Postives = 150/259 (57.92%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGV---QVAVAEDSERLQLLEPFTPIQNEALKGVKLLIK-----------AYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAP---DKPLTLEIVHADGT 878
            V  K F+  L F+P  D+L   +G+  +F  P G ELP  GF   D+ ++ PE +   V   +V +   S+RL+ LEPF P Q   ++ +K+ +K           A G WL+++GHL NI NNTLIGA+NA   + N   +   G+   +P+    +K  G   +VV +HNYGEGS+REHAA+QPR +    +L KSFARIHETNLKKQG+L LTF NE+DY+ I  +D  +   I        D+P+TL +   DG+
Sbjct:  506 VAAKIFSSDLAFDPTHDTLQTPDGKAFKFRPPQGVELPSAGFIAGDSSYI-PEPNPQPVPETEVTIDPKSDRLEALEPFEPYQGGEMENLKVAVKVKGKCTTDHISAAGKWLKYKGHLSNICNNTLIGAMNAATGEVNRAYDN--GKGMTIPELMWKWKKDGQPWLVVAEHNYGEGSAREHAALQPRAMNGRIILTKSFARIHETNLKKQGVLPLTFVNEADYEKIDAEDKVSTRGIEQLLEGVLDQPITLVVTKKDGS 761          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|728783|sp|P39533.1|ACON2_YEAST (RecName: Full=Homocitrate dehydratase, mitochondrial; AltName: Full=Aconitase 2; Flags: Precursor)

HSP 1 Score: 652.129 bits (1681), Expect = 0.000e+0
Identity = 348/667 (52.17%), Postives = 446/667 (66.87%), Query Frame = 0

Query:    1 MANRVDKAREIVGK-PLTLSEKILYAHLWDGTPS-----KAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEH---LTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPK-NSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEAD---GSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLL----------IKAYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            +   +DK ++I    PLTL+EKILY+HL D   S      + +RG  Y+   PDR+A QDA+AQMALLQFM  G  + +VP ++HCDHLI  K+G   DL  + +T+ EVFDFLES + +YGI FW PG+GIIHQ+VLEN++ PG MM+GTDSHT NAGGLG +AIGVGGADAVD + G  WELK PK++GV+LTG ++GW+ PKDVI K+A +LTV+GGTG IVEYFG G   LSCTG  TICNMGAEIGATTSTF Y  + +RYL+AT+RA+VA+AA+        L AD D        +D+VI+IDLS + P +NGPFTPDLST + +    K+ +  WP +V  GLIGSCTNSSY+D+SR   + +QA   GLK +    + PGSEQ+R T ERDGI+ +F++ G+K+  NACGPCIGQW R    K + + N+I  SFNRNF  R DGN NT  F+ SPEI  A++ +G   FNPLTDS+   NG++ +F  P G ELP +GFE     F  PE D    S V++ V  +S+RLQLLEPF P    ELK   LL          I A G WL+++GHL+NI+ NTLIGA N    + N   + L G    +P     +K+ G    V+ +HNYGEGS+REHAA+ PR LG   +LVKSFAR
Sbjct:   38 LLTNLDKIKQITNNAPLTLAEKILYSHLCDPEESITSSDLSTIRGNKYLKLNPDRVAMQDASAQMALLQFMTTGLNQTSVPASIHCDHLIVGKDGETKDLPSSIATNQEVFDFLESCAKRYGIQFWGPGSGIIHQIVLENFSAPGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALTGTPWELKAPKILGVKLTGKLNGWSTPKDVITKLAGLLTVRGGTGYIVEYFGEGVSTLSCTGMATICNMGAEIGATTSTFPYQEAHKRYLQATNRAEVAEAADVALNKFNFLRADKDA------QYDKVIEIDLSAIEPHVNGPFTPDLSTPI-SQYAEKSLKENWPQKVSAGLIGSCTNSSYQDMSRVVDLVKQASKAGLKPRIPFFVTPGSEQIRATLERDGIIDIFQENGAKVLANACGPCIGQWNREDVSKTSKETNTIFTSFNRNFRARNDGNRNTMNFLTSPEIVTAMSYSGDAQFNPLTDSIKLPNGKDFKFQPPKGDELPKRGFEHGRDKFY-PEMDPKPDSNVEIKVDPNSDRLQLLEPFKPWNGKELKTNVLLKVEGKCTTDHISAAGVWLKYKGHLENISYNTLIGAQNKETGEVNKAYD-LDGTEYDIPGLMMKWKSDGRPWTVIAEHNYGEGSAREHAALSPRFLGGEILLVKSFAR 695          

HSP 2 Score: 189.889 bits (481), Expect = 5.440e-49
Identity = 113/239 (47.28%), Postives = 142/239 (59.41%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEAD---GSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLL----------IKAYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDD---TFNFTDI 859
            V   S++    FNPLTDS+   NG++ +F  P G ELP +GFE     F  PE D    S V++ V  +S+RLQLLEPF P   + LK   LL          I A G WL+++GHL+NI+ NTLIGA N    + N   + L G    +P     +K+ G    V+ +HNYGEGS+REHAA+ PR LG   +LVKSFARIHETNLKKQG+L LTF NESDYD I   D   T N  D+
Sbjct:  498 VTAMSYSGDAQFNPLTDSIKLPNGKDFKFQPPKGDELPKRGFEHGRDKFY-PEMDPKPDSNVEIKVDPNSDRLQLLEPFKPWNGKELKTNVLLKVEGKCTTDHISAAGVWLKYKGHLENISYNTLIGAQNKETGEVNKAYD-LDGTEYDIPGLMMKWKSDGRPWTVIAEHNYGEGSAREHAALSPRFLGGEILLVKSFARIHETNLKKQGVLPLTFANESDYDKISSGDVLETLNLVDM 734          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|74596317|sp|Q5B6D6.1|ACON2_EMENI (RecName: Full=Putative aconitate hydratase, mitochondrial; AltName: Full=Aconitase 2; Flags: Precursor)

HSP 1 Score: 648.277 bits (1671), Expect = 0.000e+0
Identity = 341/671 (50.82%), Postives = 440/671 (65.57%), Query Frame = 0

Query:    1 MANRVDKAREIVG--KPLTLSEKILYAHL-------WDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFL--APEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLL-----------IKAYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFN 649
            +   +++ R+++G  + LTL+EKILY+HL         GT +   +RGK  +   PDR+A QDA+AQMALLQFM  G    AVP ++HCDH+I  + GA  DL  +   + EVFDFLES S +YGI FW PGAGIIHQ VLENY+ PG MM+GTDSHT NAGGLG +AIGVGGADAVD +    WELK P+++GVRL G + GW APKD+IL +A  LTV+GGTG ++EY GPG   LS TG  TICNMGAE+GATTS F +  +   YL+AT+RADVA+AA ++    ++   + A+    +D++I IDLS L P +NGPFTPDLS  + +       +N WP     GLIGSCTNSSYED++RA  + +QA   GLK KA+L I PGSEQ+R T +RD  LS F   G  +  NACGPCIGQW R  D      N+I  S+NRNF  R DGN  T  F+ASPE+  A+  AG   FNP+TDS+   +G E RF+ PTG +LP KGFE  +  F   AP  D S V+V V+  S RL LLEPF P  +++L+ + +L           I A GPWL+++GHL NI+ NTLIGAVNA   +TN   ++  G+   +PD    +KA G + +VV + NYGEGS+REHAA+QPR+LG   +L KSFAR  + N
Sbjct:   47 IVQNLEQVRKVLGSSRALTLAEKILYSHLDNAEESLLTGTNNGRDIRGKADLKLKPDRVAMQDASAQMALLQFMSCGLPSTAVPASIHCDHMIVGERGADTDLPASIQGNKEVFDFLESASKRYGIEFWPPGAGIIHQSVLENYSAPGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALVDAPWELKAPRILGVRLEGKLQGWAAPKDIILHLAGKLTVRGGTGFVIEYHGPGVETLSTTGMATICNMGAEVGATTSLFPFSPNHVPYLKATNRADVAEAAAKIASAGSSSL-LRADTSAEYDELITIDLSTLEPHINGPFTPDLSVPL-SRFAETVRKNNWPETFNAGLIGSCTNSSYEDMTRAEHLVKQANAAGLKPKADLFITPGSEQIRATLDRDQTLSTFSSAGGTVLANACGPCIGQWKRTDDVPKGTDNAIFTSYNRNFPGRNDGNRRTMNFLASPELVTALTYAGSTTFNPVTDSITTPSGSEFRFEPPTGQDLPSKGFEAGNPAFQPSAPVPD-SSVEVKVSPTSTRLALLEPFAPFPNSDLQNLSVLYKVKGQCTTDTISAAGPWLKYKGHLPNISANTLIGAVNAATGETNVAYDE-AGKQHTIPDLAAQWKAQGREWLVVAEENYGEGSAREHAALQPRYLGGRVILAKSFARIHETN 713          

HSP 2 Score: 205.682 bits (522), Expect = 2.489e-54
Identity = 111/229 (48.47%), Postives = 146/229 (63.76%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFL--APEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLL-----------IKAYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDD 852
            V   ++A    FNP+TDS+   +G E RF+ PTG +LP KGFE  +  F   AP  D S V+V V+  S RL LLEPF P  N  L+ + +L           I A GPWL+++GHL NI+ NTLIGAVNA   +TN   ++  G+   +PD    +KA G + +VV + NYGEGS+REHAA+QPR+LG   +L KSFARIHETNLKKQG++ LTF +++DYD I   D
Sbjct:  511 VTALTYAGSTTFNPVTDSITTPSGSEFRFEPPTGQDLPSKGFEAGNPAFQPSAPVPDSS-VEVKVSPTSTRLALLEPFAPFPNSDLQNLSVLYKVKGQCTTDTISAAGPWLKYKGHLPNISANTLIGAVNAATGETNVAYDE-AGKQHTIPDLAAQWKAQGREWLVVAEENYGEGSAREHAALQPRYLGGRVILAKSFARIHETNLKKQGVVPLTFADKADYDRIDACD 737          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|74669593|sp|Q4WJ90.1|ACON2_ASPFU (RecName: Full=Putative aconitate hydratase, mitochondrial; AltName: Full=Aconitase 2; Flags: Precursor)

HSP 1 Score: 641.343 bits (1653), Expect = 0.000e+0
Identity = 345/676 (51.04%), Postives = 436/676 (64.50%), Query Frame = 0

Query:    1 MANRVDKAREIVG--KPLTLSEKILYAHL-------WDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEV-----KEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEA--DGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLL-----------IKAYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFN 649
            +   +++ R+++G  + LTL+EKILYAHL         GT +   +RGK  +   PDR+A QDA+AQMALLQFM  G    AVP ++HCDH+I  + GA  DL  +   + EVFDFLES + +YGI FW PGAGIIHQ VLENYA PG MM+GTDSHT NAGGLG +AIGVGGADAVD +    WELK P+++GVRL G +SGW +PKD+IL +A  LTV+GGTG ++EY GPG   LSCTG  T CNMGAE+GATTS F +  SM  YL+AT R  VA AA E+     K  L AD          +DQ+I IDLS L P +NGPFTPDLS  + +D      EN+WP  +  GLIGSCTNSSYED++RA  + +QA   GLK KA+  I PGSEQ+R T +RD  L+ F + G  +  NACGPCIGQW R         N+I  S+NRNF  R DGN  T  F+ASPEI  A+A +G   FNP+TD+L   +G+E +F  P G +LP  GF  +    L P A    + V+V V+  SERL LLEPF P  + EL G+K+L           I A GPWL+++GHL NI+ NTLIGAVNA   +TN   +   G    +PD    +KA GI+ +VV + NYGEGS+REHAA+QPR+LG   ++ KSFAR  + N
Sbjct:   47 IVRNLEQVRKVLGSSRALTLAEKILYAHLDNAEESLLTGTNNGKDIRGKANLKLKPDRVAMQDASAQMALLQFMSCGLPSTAVPASIHCDHMIVGERGADTDLPASIEGNREVFDFLESAAKRYGIEFWPPGAGIIHQSVLENYAAPGLMMLGTDSHTPNAGGLGAIAIGVGGADAVDALVDAPWELKAPRILGVRLEGRLSGWASPKDIILHLAGKLTVRGGTGYVIEYHGPGVETLSCTGMATCCNMGAEVGATTSVFPFSPSMVPYLQATHRGHVAQAAAEIAASGPKNLLRADDGA------EYDQLITIDLSTLEPHVNGPFTPDLSVRL-SDFANTVRENKWPETLGAGLIGSCTNSSYEDMTRAEDLVKQASAAGLKPKADFFITPGSEQIRATLDRDQTLASFSEAGGTVLANACGPCIGQWKRTDGVAKGEDNAIFTSYNRNFPGRNDGNRRTMNFLASPEIVTALAYSGSTTFNPMTDTLKTPSGEEFKFRPPQGSDLPSAGF-ADGNPALQPSAGVPDASVEVIVSPTSERLALLEPFAPFPEGELSGLKVLYKVKGQCTTDTISAAGPWLKYKGHLPNISANTLIGAVNAATGETNVAYDD-AGNKHSIPDLAARWKADGIEWLVVAEDNYGEGSAREHAALQPRYLGGRIIVAKSFARIHETN 713          

HSP 2 Score: 194.126 bits (492), Expect = 2.433e-50
Identity = 112/262 (42.75%), Postives = 153/262 (58.40%), Query Frame = 0

Query:  637 VLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEA--DGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLL-----------IKAYGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLI---QEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTI 882
            V   +++    FNP+TD+L   +G+E +F  P G +LP  GF  +    L P A    + V+V V+  SERL LLEPF P     L G+K+L           I A GPWL+++GHL NI+ NTLIGAVNA   +TN   +   G    +PD    +KA GI+ +VV + NYGEGS+REHAA+QPR+LG   ++ KSFARIHETNLKKQG++ LTF +++DYD I    + DT    +         + L++    G K TI
Sbjct:  511 VTALAYSGSTTFNPMTDTLKTPSGEEFKFRPPQGSDLPSAGF-ADGNPALQPSAGVPDASVEVIVSPTSERLALLEPFAPFPEGELSGLKVLYKVKGQCTTDTISAAGPWLKYKGHLPNISANTLIGAVNAATGETNVAYDD-AGNKHSIPDLAARWKADGIEWLVVAEDNYGEGSAREHAALQPRYLGGRIIVAKSFARIHETNLKKQGVVPLTFADKADYDRIDACDQVDTVGLYETLQSGGQGSIQLQVTKKSGEKLTI 770          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|122953533|sp|Q0QLE2.1|DMDA_EUBBA (RecName: Full=2,3-dimethylmalate dehydratase large subunit)

HSP 1 Score: 166.392 bits (420), Expect = 2.424e-43
Identity = 141/460 (30.65%), Postives = 214/460 (46.52%), Query Frame = 0

Query:   16 LTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHC--DHLIQAKNGAAADLKFANST-SAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLEN-YAFPGGMMIGTDSHTVNAGGLGMVAIGVGGAD-AVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVY-ANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATEN-EWPIQVEWGLIGSCTNSSYEDLSRASSIAQ-QALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRL 467
            +T+++KIL AH      S   ++  D +    D +   D T  +A+  F    K+KV     +    DH   AK     D+K A        F   + ++N + IG      GI H ++ E      G ++IG DSHT   G LG  + GVG  D AV +  G AW  K P  +   LTGT++   + KD+IL +  ++ V G     +E+ GPG   LS   + TI NM  E G     F  D     Y+               +EH + D  VY A+ +  +++  DIDLS+L   +  P  P+         N K  +  E P+ ++  +IGSCTN  +EDL RA+ I + + + KG++M     + P + ++   A   G L  F + G+ I T  CGPC+G +        A     V + NRNF  R  G+ ++  ++ASPE+ AA AI GR+
Sbjct:    3 MTMTQKILAAH-----ASLDSVKAGDLIMADLDMVLANDITGPVAINVFGTIDKEKVFDKDKIALVPDHFAPAK-----DIKSAQQCKQVRCFACDQEITNYFEIGEM----GIEHALLPEKGLVAAGDVVIGADSHTCTYGALGAFSTGVGSTDMAVGMATGKAW-FKVPAALRFNLTGTLNKNVSGKDLILHIIGMIGVDGALYRSMEFTGPGVACLSMDDRFTISNMAIEAGGKNGIFPVDDQTISYM---------------EEHGSGDYKVYAADADAVYEKTFDIDLSQLKSTVAFPHLPE---------NTKTVDAIEEPVTIDQVVIGSCTNGRFEDLKRAADILRGKHVKKGVRML----VIPATHKIYLDAMEAGYLREFIEAGATISTPTCGPCLGGYMGIL----AEGERCVSTTNRNFVGRM-GHVDSEVYLASPEVAAASAILGRI 414          

BLAST of aconitate hydratase vs. SwissProt
Match: gi|3123225|sp|P21399.3|ACOC_HUMAN (RecName: Full=Cytoplasmic aconitate hydratase; Short=Aconitase; AltName: Full=Citrate hydro-lyase; AltName: Full=Ferritin repressor protein; AltName: Full=Iron regulatory protein 1; Short=IRP1; AltName: Full=Iron-responsive element-binding protein 1; Short=IRE-BP 1)

HSP 1 Score: 172.17 bits (435), Expect = 6.827e-43
Identity = 159/521 (30.52%), Postives = 232/521 (44.53%), Query Frame = 0

Query:   43 VDFAPDRIACQDATAQMALLQF--MQAGKKKV---------AVPTTVHCDHLIQAKNGAAAD-------LKFANSTSAEVFDFLESVSNKY-GIGFWKPGAGIIHQVVLE----------NYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADA------DVYANPENYFDQVIDIDLSKLGPLLNGPFTP-----------DLSTAVGTDMNVKATE----------------NEWPI---QVEWGLIGSCTNSSYEDLSR-ASSIAQQALDKGLKMKAEL--GINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDP--KNAPKNSIVH----SFNRNFAKRADGNPNTHA-FVASPEITAAIAIAG--RLDF--NPLTDSLINENGQEV 484
            V F P R+  QD T   A++ F  M+   KK+           P  +  DH IQ      AD       L+F  +   E F+FL+  S  +  +    PG+GIIHQV LE           Y +P  + +GTDSHT    GLG++  GVGG +A  VM G    +  P++IG RL G         D++L + + L   G  G  VE+FGPG   LS   + TI NM  E GAT + F  D     YL  T R +  +    +K++L A        D   +P+  F QV+++DL  + P  +GP  P           D  + +G     K  +                 E+ +    V    I SCTN+S   +   A  +A++A+D GL +   +   ++PGS  V +  +  G++    +LG  +    C  CIG      +P  +   +  +V     S NRNF  R   +PNT A ++ASP +  A AIAG  R+DF   PL    +N  GQ+V
Sbjct:   76 VPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNR--ERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSL-VGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDE--EKLKYIKKYLQAVGMFRDFNDPSQDPD--FTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRV--HPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLG---VNAKGQQV 584          

HSP 2 Score: 59.3066 bits (142), Expect = 1.218e-7
Identity = 31/73 (42.47%), Postives = 43/73 (58.90%), Query Frame = 0

Query:  767 TGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTF 839
            +GE   V D    Y+ AG+  IV+    YG GSSR+ AA  P  LG+ AVL +S+ RIH +NL   G++ L +
Sbjct:  746 SGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEY 818          

BLAST of aconitate hydratase vs. nr
Match: gi|652763555|ref|WP_027078741.1| (aconitate hydratase [Maribacter antarcticus])

HSP 1 Score: 1251.5 bits (3237), Expect = 0.000e+0
Identity = 603/655 (92.06%), Postives = 629/655 (96.03%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            MA+RVDKAREIVGKPLTLSEKILY+HLW+GTP+KAF RGKDYVDF PDRIA QDATAQMALLQFMQAGKK+VAVPTTVHCDHLIQAKNGA ADLKFANSTSAEVF+FLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAI+LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDR+DVADAANEVKEHLTADA+VYANPENYFDQVIDIDLSKLGPLLNGPFTPDLST VG+DM  KAT+N+WPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMK+ELGINPGSEQVRFTAERDGIL+VFEKL +KIFTNACGPCIGQWARYS+PKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TDSLINENGQEVRFDEPTGWELPP GFEV+DAGFLAPEADGSGVQV VA DSERLQ+LEPF PIQ+ EL+GVKLLIKA+           GPWLRFRGHLDNIANNTLIGAVNA+NKKTNFVKNQ TGEF+GVPDTQRAYKAAGIK+IV+GDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR
Sbjct:   15 MADRVDKAREIVGKPLTLSEKILYSHLWEGTPTKAFSRGKDYVDFNPDRIALQDATAQMALLQFMQAGKKQVAVPTTVHCDHLIQAKNGATADLKFANSTSAEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAISLSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRSDVADAANEVKEHLTADAEVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTPVGSDMTEKATKNDWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKSELGINPGSEQVRFTAERDGILNVFEKLDAKIFTNACGPCIGQWARYSNPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPITDSLINENGQEVRFDEPTGWELPPNGFEVQDAGFLAPEADGSGVQVVVAGDSERLQVLEPFLPIQNEELQGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNKKTNFVKNQATGEFEGVPDTQRAYKAAGIKTIVIGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 446.047 bits (1146), Expect = 7.638e-141
Identity = 213/253 (84.19%), Postives = 229/253 (90.51%), Query Frame = 0

Query:  641 SFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTI 882
            + A RLDFNP+TDSLINENGQEVRFDEPTGWELPP GFEV+DAGFLAPEADGSGVQV VA DSERLQ+LEPF PIQNE L+GVKLLIKA+G           PWLRFRGHLDNIANNTLIGAVNA+NKKTNFVKNQ TGEF+GVPDTQRAYKAAGIK+IV+GDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTF  ESDYDLI+EDDTFNF +IA FAPDKPL +E+VHADG+KDT+
Sbjct:  476 AIAGRLDFNPITDSLINENGQEVRFDEPTGWELPPNGFEVQDAGFLAPEADGSGVQVVVAGDSERLQVLEPFLPIQNEELQGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNKKTNFVKNQATGEFEGVPDTQRAYKAAGIKTIVIGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFAKESDYDLIKEDDTFNFINIADFAPDKPLIIEVVHADGSKDTV 728          

BLAST of aconitate hydratase vs. nr
Match: gi|1274386935|ref|WP_099573898.1| (aconitate hydratase [Maribacter sp. 4U21] >gi|1273375918|gb|PIB27720.1| aconitate hydratase [Maribacter sp. 4U21])

HSP 1 Score: 1232.24 bits (3187), Expect = 0.000e+0
Identity = 594/655 (90.69%), Postives = 619/655 (94.50%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            MA RVDKAREIVGKPLTLSEKILY+HLWDG PSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAKNGAAADLKFAN++SAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTG ISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGA ALSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRADVADAAN+VK++LTAD DVYANPE YFDQVIDIDL++L PLLNGPFTPDLST VG+DM  KAT+N+WPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDK LKMKAELGINPGSEQVRFTAERDGIL +FE L +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIA+AGRLDFNP+ D+LINENG+EVRFDEPTGWELPPKGFEVEDAGFLAP  DGSGV+V V + SERLQLLEPF PI++ EL+GVKLLIKA+           GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGE+ GVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR
Sbjct:   15 MAERVDKAREIVGKPLTLSEKILYSHLWDGNPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKNGAAADLKFANTSSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGNISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAKALSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADVADAANKVKDYLTADEDVYANPEQYFDQVIDIDLTELNPLLNGPFTPDLSTPVGSDMTEKATKNDWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKALKMKAELGINPGSEQVRFTAERDGILGIFEDLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAVAGRLDFNPMKDTLINENGEEVRFDEPTGWELPPKGFEVEDAGFLAPMEDGSGVKVVVDDSSERLQLLEPFVPIKNEELQGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEYGGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 448.743 bits (1153), Expect = 6.742e-142
Identity = 228/306 (74.51%), Postives = 248/306 (81.05%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  AA+ V   A RLDFNP+ D+LINENG+EVRFDEPTGWELPPKGFEVEDAGFLAP  DGSGV+V V + SERLQLLEPF PI+NE L+GVKLLIKA+G           PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGE+ GVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTF NESDYDLIQEDDTFNF DI  FAPDKPLTLEIVHADG+KDTIK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEI-TAAIAV---AGRLDFNPMKDTLINENGEEVRFDEPTGWELPPKGFEVEDAGFLAPMEDGSGVKVVVDDSSERLQLLEPFVPIKNEELQGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEYGGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFANESDYDLIQEDDTFNFIDIVDFAPDKPLTLEIVHADGSKDTIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|1236838942|ref|WP_094995649.1| (aconitate hydratase [Maribacter cobaltidurans] >gi|1236397146|gb|ASV29015.1| aconitate hydratase [Maribacter cobaltidurans])

HSP 1 Score: 1219.53 bits (3154), Expect = 0.000e+0
Identity = 589/655 (89.92%), Postives = 615/655 (93.89%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            MA RVDKAR++VGKPLTLSEKILYAHLWDG+PSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK ANS+SAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTG ISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGA ALSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRADVADAAN+VKE+LTAD +VYANPE YFDQVIDIDLSKL PLLNGPFTPDLST VG +M  KAT+N+WP+QVEWGLIGSCTNSSYEDLSRASSIAQQALDK LK KAE GINPGSEQVR+TAERDGIL +FEKL +KIFTNACGPCIGQWARY+DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD+LINE GQEVRFDEPTGWELPPKGFEVEDAG+LAP  DGS V+V VA DSERLQLLEPF PI + EL+GVKLLIKAY           GPWLRFRGHLDNIANNTLIGAVNA+NKKTN VKNQLTGE++GVPDTQRAYKA GIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR
Sbjct:   15 MAERVDKARDLVGKPLTLSEKILYAHLWDGSPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANSSSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGEISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAKALSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADVADAANQVKEYLTADDEVYANPEQYFDQVIDIDLSKLNPLLNGPFTPDLSTPVGKEMTKKATDNDWPMQVEWGLIGSCTNSSYEDLSRASSIAQQALDKNLKTKAEFGINPGSEQVRYTAERDGILGIFEKLDAKIFTNACGPCIGQWARYNDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDTLINEEGQEVRFDEPTGWELPPKGFEVEDAGYLAPREDGSDVKVVVAPDSERLQLLEPFEPITNEELQGVKLLIKAYGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNKKTNLVKNQLTGEYEGVPDTQRAYKAKGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 444.506 bits (1142), Expect = 2.310e-140
Identity = 214/254 (84.25%), Postives = 227/254 (89.37%), Query Frame = 0

Query:  641 SFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            + A RLDFNP+TD+LINE GQEVRFDEPTGWELPPKGFEVEDAG+LAP  DGS V+V VA DSERLQLLEPF PI NE L+GVKLLIKAYG           PWLRFRGHLDNIANNTLIGAVNA+NKKTN VKNQLTGE++GVPDTQRAYKA GIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTF NE+DYDLIQEDDTFNF DIA FAPDKPLT+E+VHADG+KDTIK
Sbjct:  476 AIAGRLDFNPMTDTLINEEGQEVRFDEPTGWELPPKGFEVEDAGYLAPREDGSDVKVVVAPDSERLQLLEPFEPITNEELQGVKLLIKAYGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNKKTNLVKNQLTGEYEGVPDTQRAYKAKGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFANENDYDLIQEDDTFNFVDIADFAPDKPLTIEVVHADGSKDTIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|1274352022|ref|WP_099543217.1| (aconitate hydratase [Maribacter sp. 4G9] >gi|1273387565|gb|PIB38911.1| aconitate hydratase [Maribacter sp. 4G9])

HSP 1 Score: 1213.36 bits (3138), Expect = 0.000e+0
Identity = 585/655 (89.31%), Postives = 615/655 (93.89%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            MA RVDKAREIVGKPLTLSEKILYAHLWDGTP++AF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK ANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTG ISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRADVADAAN+V+E+LTAD +VYANPE YFDQVIDIDLS L PLLNGPFTPDLST VG +M  KAT+N+WP+QVEWGLIGSCTNSSYEDLSRASSIAQQALDK LK KAE GINPGSEQVR+TAERDGIL +FEKL +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD+LINE+G+EVRF+EPTGWELPPKGFEVEDAG+LAP+ DGS V+V V   SERLQLLEPF PI++ EL+ VKLLIKAY           GPWLRFRGHLDNIANNTLIGAVNA+N+KTN VKNQLTGE++GVPDTQRAYKA GIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR
Sbjct:   15 MAERVDKAREIVGKPLTLSEKILYAHLWDGTPTRAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGKISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADVADAANKVREYLTADDEVYANPEQYFDQVIDIDLSTLNPLLNGPFTPDLSTPVGKEMTEKATKNDWPMQVEWGLIGSCTNSSYEDLSRASSIAQQALDKKLKTKAEFGINPGSEQVRYTAERDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDTLINEDGEEVRFEEPTGWELPPKGFEVEDAGYLAPKEDGSSVKVVVDPTSERLQLLEPFEPIKNEELQDVKLLIKAYGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNQKTNLVKNQLTGEYEGVPDTQRAYKAKGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 427.172 bits (1097), Expect = 1.188e-133
Identity = 212/306 (69.28%), Postives = 243/306 (79.41%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    A RLDFNP+TD+LINE+G+EVRF+EPTGWELPPKGFEVEDAG+LAP+ DGS V+V V   SERLQLLEPF PI+NE L+ VKLLIKAYG           PWLRFRGHLDNIANNTLIGAVNA+N+KTN VKNQLTGE++GVPDTQRAYKA GIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGML LTF NE+DYDLI+EDDTFNF DI+ FAPD PLT+E+VHADG+KD+IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----AGRLDFNPMTDTLINEDGEEVRFEEPTGWELPPKGFEVEDAGYLAPKEDGSSVKVVVDPTSERLQLLEPFEPIKNEELQDVKLLIKAYGKCTTDHISMAGPWLRFRGHLDNIANNTLIGAVNAFNQKTNLVKNQLTGEYEGVPDTQRAYKAKGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLALTFANENDYDLIKEDDTFNFVDISDFAPDTPLTIEVVHADGSKDSIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|1124238831|ref|WP_074538938.1| (aconitate hydratase [Cellulophaga baltica] >gi|1086759724|emb|SDF23318.1| aconitase [Cellulophaga baltica])

HSP 1 Score: 1212.59 bits (3136), Expect = 0.000e+0
Identity = 582/655 (88.85%), Postives = 615/655 (93.89%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M++RV+KARE+VGKPLTLSEKILY+HLWDGTPSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK AN+TSAEVF+FLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFG GA +LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAANEV+EHLTADADVYANPE YFD+VI+I+LS+L PLLNGPFTPDLST VG+DM  KATENEWP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMK+ELGINPGSEQVRFTA RDGIL VFE L +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+D  L GVKLLIKA+           GPWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFAR
Sbjct:   15 MSDRVNKAREVVGKPLTLSEKILYSHLWDGTPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANNTSAEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGSGATSLSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADIADAANEVREHLTADADVYANPEQYFDEVIEINLSELTPLLNGPFTPDLSTKVGSDMTEKATENEWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKSELGINPGSEQVRFTAARDGILQVFENLNAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDKNLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 426.787 bits (1096), Expect = 1.634e-133
Identity = 214/306 (69.93%), Postives = 240/306 (78.43%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    A RLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+++ L GVKLLIKA+G           PWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFARIHETNLKKQGMLGLTF  ESDYDLIQEDDTFNF DIA FAPDK LT+E+VHADG+KD IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----AGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDKNLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFATESDYDLIQEDDTFNFIDIAEFAPDKQLTIEVVHADGSKDVIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|639136006|ref|WP_024481492.1| (aconitate hydratase [Cellulophaga baltica])

HSP 1 Score: 1212.21 bits (3135), Expect = 0.000e+0
Identity = 581/655 (88.70%), Postives = 616/655 (94.05%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M++RV+KARE+VGKPLTLSEKILY+HLWDGTPSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK AN+TSAEVF+FLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFG GA +LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAANEV+EHLTADA+VYANPE YFD+VI+I+LS+L PLLNGPFTPDLST VG+DM  KATENEWP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMK+ELGINPGSEQVRFTA RDGIL VFE L +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+D  L GVKLLIKA+           GPWLRFRGHLDNI+NN LIGAVNA+ +KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFAR
Sbjct:   15 MSDRVNKAREVVGKPLTLSEKILYSHLWDGTPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANNTSAEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGSGATSLSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADIADAANEVREHLTADAEVYANPEQYFDEVIEINLSELTPLLNGPFTPDLSTKVGSDMTEKATENEWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKSELGINPGSEQVRFTAARDGILQVFENLNAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDESLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGQKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 430.254 bits (1105), Expect = 6.413e-135
Identity = 215/306 (70.26%), Postives = 242/306 (79.08%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    A RLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI++E+L GVKLLIKA+G           PWLRFRGHLDNI+NN LIGAVNA+ +KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFARIHETNLKKQGMLGLTF  ESDYDLIQEDDTFNF DIA FAPDK LT+E+VHADG+KD IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----AGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDESLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGQKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFATESDYDLIQEDDTFNFIDIAEFAPDKQLTIEVVHADGSKDVIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|657645372|ref|WP_029449842.1| (aconitate hydratase [Cellulophaga baltica])

HSP 1 Score: 1211.05 bits (3132), Expect = 0.000e+0
Identity = 581/655 (88.70%), Postives = 615/655 (93.89%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M++RV+KARE+VGKPLTLSEKILY+HLWDGTPSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK AN+TSAEVF+FLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFG GA +LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAANEV+EHLTADA+VYANPE YFD+VI+I+LS+L PLLNGPFTPDLST VG+DM  KATENEWP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMK+ELGINPGSEQVRFTA RDGIL VFE L +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+D  L GVKLLIKA+           GPWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFAR
Sbjct:   15 MSDRVNKAREVVGKPLTLSEKILYSHLWDGTPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANNTSAEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGSGATSLSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADIADAANEVREHLTADAEVYANPEQYFDEVIEINLSELTPLLNGPFTPDLSTKVGSDMTEKATENEWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKSELGINPGSEQVRFTAARDGILQVFENLNAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDENLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 428.713 bits (1101), Expect = 3.433e-134
Identity = 215/306 (70.26%), Postives = 240/306 (78.43%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    A RLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI++E L GVKLLIKA+G           PWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFARIHETNLKKQGMLGLTF  ESDYDLIQEDDTFNF DIA FAPDK LT+E+VHADG+KD IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----AGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDENLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFATESDYDLIQEDDTFNFIDIAEFAPDKQLTIEVVHADGSKDVIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|646943766|ref|WP_025615707.1| (MULTISPECIES: aconitate hydratase [Cellulophaga] >gi|694993226|gb|KGK31013.1| aconitate hydratase [Cellulophaga sp. E6(2014)] >gi|723620072|gb|AIY13444.1| aconitate hydratase [Cellulophaga baltica NN016038] >gi|730599000|gb|AIZ41795.1| aconitate hydratase [Cellulophaga baltica 18])

HSP 1 Score: 1210.67 bits (3131), Expect = 0.000e+0
Identity = 580/655 (88.55%), Postives = 615/655 (93.89%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M++RV+KARE+VGKPLTLSEKILY+HLWDGTPSKAF+RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GAAADLK AN+TSAEVF+FLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFG GA +LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAANEV+EHLTADA+VYANPE YFD+VI+I+L++L PLLNGPFTPDLST VG+DM  KATENEWP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMK+ELGINPGSEQVRFTA RDGIL VFE L +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+D  L GVKLLIKA+           GPWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFAR
Sbjct:   15 MSDRVNKAREVVGKPLTLSEKILYSHLWDGTPSKAFVRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGAAADLKSANNTSAEVFNFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGSGATSLSCTGKGTICNMGAEIGATTSTFGYDDSMERYLRATDRADIADAANEVREHLTADAEVYANPEQYFDEVIEINLTELTPLLNGPFTPDLSTKVGSDMTEKATENEWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKSELGINPGSEQVRFTAARDGILQVFENLNAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDENLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 428.713 bits (1101), Expect = 3.255e-134
Identity = 215/306 (70.26%), Postives = 240/306 (78.43%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    A RLDFNP+TD LINENG+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI++E L GVKLLIKA+G           PWLRFRGHLDNI+NN LIGAVNA+  KTNFVKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFARIHETNLKKQGMLGLTF  ESDYDLIQEDDTFNF DIA FAPDK LT+E+VHADG+KD IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----AGRLDFNPMTDKLINENGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVATDSERLQLLEPFTPIKDENLMGVKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNFVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFATESDYDLIQEDDTFNFIDIAEFAPDKQLTIEVVHADGSKDVIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|1219244663|ref|WP_089262032.1| (aconitate hydratase [Maribacter sedimenticola] >gi|1216016929|emb|SNR70143.1| aconitase [Maribacter sedimenticola])

HSP 1 Score: 1203.73 bits (3113), Expect = 0.000e+0
Identity = 577/655 (88.09%), Postives = 610/655 (93.13%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            MA RVD+AR IVGKPLTLSEKILY+HLWDGTP+KAF RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GA ADLK ANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGA +LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAAN+VKEHLTADA+VYANPE YFD+VI+IDL+ L PLLNGPFTPDLST VG+DM  KATE++WP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMKAELGINPGSEQVR+TA+RDGIL +FEKL +KIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI+GRLDFNP+TD LINE G+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI+D  L G KLLIKAY           GPWLRFRGHLDNI+NN LIGAVNA+  KTN VKNQLTGE+ GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFAR
Sbjct:   15 MAKRVDEARNIVGKPLTLSEKILYSHLWDGTPTKAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGATADLKSANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAKSLSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADIADAANKVKEHLTADAEVYANPEQYFDEVIEIDLTALTPLLNGPFTPDLSTKVGSDMTKKATEHDWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKAELGINPGSEQVRYTADRDGILGIFEKLDAKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAISGRLDFNPMTDKLINEKGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVASDSERLQLLEPFTPIKDESLMGAKLLIKAYGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNMVKNQLTGEYGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFAR 669          

HSP 2 Score: 422.935 bits (1086), Expect = 4.025e-132
Identity = 211/306 (68.95%), Postives = 239/306 (78.10%), Query Frame = 0

Query:  589 GEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            G++    D + A K + + S         +G+   HA +    +  A  +    + RLDFNP+TD LINE G+EV FDEPTGWELPPKGFEVEDAG+LAP+ DGSGV V VA DSERLQLLEPFTPI++E+L G KLLIKAYG           PWLRFRGHLDNI+NN LIGAVNA+  KTN VKNQLTGE+ GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAVLVKSFARIHETNLKKQGMLGLTF NE+DYDLIQEDDTFNF DIA FAPDK LT+E+VHADG+KD IK
Sbjct:  428 GQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI----SGRLDFNPMTDKLINEKGEEVMFDEPTGWELPPKGFEVEDAGYLAPDEDGSGVVVKVASDSERLQLLEPFTPIKDESLMGAKLLIKAYGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGHKTNMVKNQLTGEYGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFANENDYDLIQEDDTFNFLDIAEFAPDKQLTIEVVHADGSKDVIK 729          

BLAST of aconitate hydratase vs. nr
Match: gi|1134307885|ref|WP_076547862.1| (aconitate hydratase [Maribacter ulvicola] >gi|1131913522|emb|SIQ41371.1| aconitase [Maribacter ulvicola])

HSP 1 Score: 1201.81 bits (3108), Expect = 0.000e+0
Identity = 575/655 (87.79%), Postives = 610/655 (93.13%), Query Frame = 0

Query:    1 MANRVDKAREIVGKPLTLSEKILYAHLWDGTPSKAFLRGKDYVDFAPDRIACQDATAQMALLQFMQAGKKKVAVPTTVHCDHLIQAKNGAAADLKFANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVRLTGTISGWTAPKDVILKVAEILTVKGGTGAIVEYFGPGAIALSCTGKGTICNMGAEIGATTSTFGYDASMERYLRATDRADVADAANEVKEHLTADADVYANPENYFDQVIDIDLSKLGPLLNGPFTPDLSTAVGTDMNVKATENEWPIQVEWGLIGSCTNSSYEDLSRASSIAQQALDKGLKMKAELGINPGSEQVRFTAERDGILSVFEKLGSKIFTNACGPCIGQWARYSDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAIAGRLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQDAELKGVKLLIKAY-----------GPWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFAR 644
            M+ RVDKAREIVGKPLTLSEKILY+HLWDG+P++AF RGKDYVDFAPDRIACQDATAQMALLQFMQAGK KVAVPTTVHCDHLIQAK+GA ADLK ANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGV+LTG ISGWTAPKDVILKVAEILTVKGGTGAIVEYFG GA  LSCTGKGTICNMGAEIGATTSTFGYD SMERYLRATDRAD+ADAANEVKEHLTADA+VYANPE YFD+VI+IDL+KL PLLNGPFTPDLST VG+DM  KATENEWP+ VEWGLIGSCTNSSYEDLSRASSIAQQA+DKGLKMKAELGINPGSEQVR+TA+RDGIL +FEKL +KIFTNACGPCIGQWARY+DPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAI+GRLDFNP+TD LIN+ G+EV FDEPTGWELPPKGFEVEDAG+LAP  DGSGV+V VA +SERLQLLEPFTPI+D  L G KLLIKA+           GPWLRFRGHLDNI+NN LIGAVNA+ KKTN VKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAV+VKSFAR
Sbjct:   15 MSERVDKAREIVGKPLTLSEKILYSHLWDGSPTEAFTRGKDYVDFAPDRIACQDATAQMALLQFMQAGKPKVAVPTTVHCDHLIQAKSGATADLKSANSTSAEVFDFLESVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMVAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGDISGWTAPKDVILKVAEILTVKGGTGAIVEYFGEGAKNLSCTGKGTICNMGAEIGATTSTFGYDESMERYLRATDRADIADAANEVKEHLTADAEVYANPEQYFDEVIEIDLAKLTPLLNGPFTPDLSTKVGSDMTEKATENEWPLAVEWGLIGSCTNSSYEDLSRASSIAQQAIDKGLKMKAELGINPGSEQVRYTADRDGILGIFEKLDAKIFTNACGPCIGQWARYNDPKNAPKNSIVHSFNRNFAKRADGNPNTHAFVASPEITAAIAISGRLDFNPMTDKLINQKGEEVMFDEPTGWELPPKGFEVEDAGYLAPRKDGSGVEVKVASNSERLQLLEPFTPIKDESLMGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGKKTNMVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFAR 669          

HSP 2 Score: 427.172 bits (1097), Expect = 1.321e-133
Identity = 202/250 (80.80%), Postives = 222/250 (88.80%), Query Frame = 0

Query:  645 RLDFNPLTDSLINENGQEVRFDEPTGWELPPKGFEVEDAGFLAPEADGSGVQVAVAEDSERLQLLEPFTPIQNEALKGVKLLIKAYG-----------PWLRFRGHLDNIANNTLIGAVNAYNKKTNFVKNQLTGEFQGVPDTQRAYKAAGIKSIVVGDHNYGEGSSREHAAMQPRHLGVAAVLVKSFARIHETNLKKQGMLGLTFDNESDYDLIQEDDTFNFTDIASFAPDKPLTLEIVHADGTKDTIK 883
            RLDFNP+TD LIN+ G+EV FDEPTGWELPPKGFEVEDAG+LAP  DGSGV+V VA +SERLQLLEPFTPI++E+L G KLLIKA+G           PWLRFRGHLDNI+NN LIGAVNA+ KKTN VKNQLTGEF GVPDT RAYKAAG++SIVVGDHNYGEGSSREHAAM+PRHLGVAAV+VKSFARIHETNLKKQGMLGLTF NE+DYDLIQEDDTFNFTDIA FAPDKPLT+EIVH+DG+KD IK
Sbjct:  480 RLDFNPMTDKLINQKGEEVMFDEPTGWELPPKGFEVEDAGYLAPRKDGSGVEVKVASNSERLQLLEPFTPIKDESLMGAKLLIKAFGKCTTDHISMAGPWLRFRGHLDNISNNCLIGAVNAFGKKTNMVKNQLTGEFGGVPDTARAYKAAGVRSIVVGDHNYGEGSSREHAAMEPRHLGVAAVIVKSFARIHETNLKKQGMLGLTFANENDYDLIQEDDTFNFTDIAEFAPDKPLTVEIVHSDGSKDVIK 729