BLAST of neprilysin-2-like isoform x3 vs. L. salmonis genes
Match: EMLSAG00000002385 (supercontig:LSalAtl2s:LSalAtl2s1453:5092:9768:-1 gene:EMLSAG00000002385 transcript:EMLSAT00000002385 description:"maker-LSalAtl2s1453-augustus-gene-0.6")

HSP 1 Score: 148.288 bits (373), Expect = 1.364e-37
Identity = 71/155 (45.81%), Postives = 95/155 (61.29%), Query Frame = 0

Query:  782 KQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEE-SILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            K  DE   L+NWW+N+T + F  ++ CFV+QY N+T+  +N TI GK    E IADNGG+  AY A+  W   + +   +LPGL+ TS QLFW S AN WCT+   EN++  +    H     RV + FSN  EFAKDF CP  SPMNP  +C++
Sbjct:  405 KSNDEISGLENWWDNQTEKNFLNRSQCFVEQYGNYTIKSVNQTINGKLTLPENIADNGGLRAAYRAFIEWKNNNTKSIRLLPGLNFTSNQLFWLSGANMWCTKYLKENLEEVLLTNEHSPTSARVVLSFSNHEEFAKDFNCPTNSPMNPPDRCRV 559          

HSP 2 Score: 125.176 bits (313), Expect = 3.967e-30
Identity = 76/254 (29.92%), Postives = 129/254 (50.79%), Query Frame = 0

Query:  122 YSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTK---KGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQS 372
            ++C GF  + + P   S V+  S L   +  +++ + +     +  R   +   F+QACM+   +E +GL+PL++ L   GGW L  K       KGT   W     KL D G++  +L  +D+  D  N +    FL +P  G+    L +G  +  V+AY+ Y  D AVLLG    ++++  L  ++ FEI +AN T+S  ++S++  L N M +  LT   P + W+QY+N + +    + QM +L +  S
Sbjct:    1 FACGGFVXKTVFPPGESQVNIFSELTTLLNNQMKTLLERNSTNKDIRPFVLVRNFYQACMNETAIETVGLKPLQEMLSRLGGW-LDTKENDDVLTKGTKYNWTSDLKKLRDHGYSTKFLMHIDISQDLSNYSRMSLFLDKPEFGIYRNALVKGRGDFEVEAYFQYMKDAAVLLGH-NFSEVEENLENILNFEIQMANLTKSNDEISNLTDLNNKMQIKNLTTLNPCIPWLQYINSLLK----INQMKTLLERNS 248          

HSP 3 Score: 96.6709 bits (239), Expect = 7.043e-21
Identity = 55/156 (35.26%), Postives = 86/156 (55.13%), Query Frame = 0

Query:  176 FFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMR---TKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDS 328
            F+QACM+   +E +GL+PL++ L   GGW L  K     + KGT   W     KL D G++ N+L  +D+  D  N +     L +P  G+    L +G  +  V+AY+ Y  D AVLLG    TD++  L  ++ FEI +AN T+S  ++S +++
Sbjct:  262 FYQACMNETAIETVGLKPLQEMLSRLGGW-LDTKETYDGSTKGTKYNWTSDLKKLRDHGYSTNFLMYIDISQDLSNYSRMSLLLDKPAFGINRNVLVKGRGDFKVEAYFQYMKDXAVLLGH-NFTDVEENLDNILNFEIQMANLTKSNDEISGLEN 415          

BLAST of neprilysin-2-like isoform x3 vs. L. salmonis genes
Match: EMLSAG00000002246 (supercontig:LSalAtl2s:LSalAtl2s14276:147:721:-1 gene:EMLSAG00000002246 transcript:EMLSAT00000002246 description:"maker-LSalAtl2s14276-augustus-gene-0.1")

HSP 1 Score: 117.472 bits (293), Expect = 1.405e-30
Identity = 63/155 (40.65%), Postives = 85/155 (54.84%), Query Frame = 0

Query:  800 EEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWV-----------------EKHGEESI--LPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            + F  ++ CFV+QY N+T+  +N TI GK    E IA NGG+  AY A+S ++                 + +  +SI  LPGL+ TS QLFW S AN WCT+   EN++  +    H     RV + FSN  EFAKDF CP  SPMNP   C++
Sbjct:   17 KNFLNRSQCFVEQYGNYTIKSVNQTINGKLTLPENIAHNGGLRAAYRAFSNYIIPDNVHIYIYIYXTVEWKNNNTKSIRLLPGLNFTSNQLFWLSGANMWCTKYLKENLEEVLLTNEHSPTSARVVLSFSNHEEFAKDFNCPTNSPMNPPDXCRV 171          

BLAST of neprilysin-2-like isoform x3 vs. L. salmonis genes
Match: EMLSAG00000010734 (supercontig:LSalAtl2s:LSalAtl2s711:278539:279118:-1 gene:EMLSAG00000010734 transcript:EMLSAT00000010734 description:"snap_masked-LSalAtl2s711-processed-gene-2.3")

HSP 1 Score: 112.849 bits (281), Expect = 4.532e-29
Identity = 61/156 (39.10%), Postives = 89/156 (57.05%), Query Frame = 0

Query:  770 GHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTS-KQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLG 924
             HE+TH FDDSG ++DE G  K  ++++T + FR ++DC   QYS+F +    L ++G    GE IAD+GG+  A  AY  W +   +   LP LD  S +Q+F+   A  WC      ++K +V    H   K+RV  P SNS +FA+ + CPLG
Sbjct:    4 AHEITHAFDDSGIKYDEYGFYKQLYDDETVKAFRKESDCIRRQYSSFQLS--GLEVDGNRTLGENIADHGGLKIAEIAYKEWKQNRSDVG-LPALDFVSDEQMFYLGYALPWCASHTKSHLKSHVFNDEHAPEKFRVLGPLSNSEDFARAYNCPLG 156          

BLAST of neprilysin-2-like isoform x3 vs. L. salmonis genes
Match: EMLSAG00000005815 (supercontig:LSalAtl2s:LSalAtl2s3137:6877:9912:1 gene:EMLSAG00000005815 transcript:EMLSAT00000005815 description:"maker-LSalAtl2s3137-snap-gene-0.3")

HSP 1 Score: 106.686 bits (265), Expect = 4.362e-25
Identity = 57/199 (28.64%), Postives = 97/199 (48.74%), Query Frame = 0

Query:  278 QAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMV 476
            QAY+ Y  D A LLG    T+++  L  ++ FEI +AN T+S  +  D+  LYN M +  LT   P + W+ Y+N   ++           Q+Q+   V+V +P Y + L   +  +    ++ Y+ WR I++S+P +N+  + ++  F    +GT S       C+   SN     VG  YV   F   +K  + +M+
Sbjct:   87 QAYFKYMKDAAXLLGH-NLTEVEGKLQNILDFEIKMANLTKSKDEKKDISVLYNKMQIKNLTNLNPCIDWLDYINSFLKTT----------QVQNEDNVIVSEPSYVKNLYQLMVDSSLEVVKDYVRWRVIESSIPNLNAAAQNISEAFSNNLNGTSSKQSIEITCIQLVSNKLKHAVGAMYVRDFFKESSKKNIKDMI 274          

HSP 2 Score: 65.4698 bits (158), Expect = 1.895e-11
Identity = 35/97 (36.08%), Postives = 54/97 (55.67%), Query Frame = 0

Query:  840 GIMHAYDAYSAWVEKHGEESILP-GLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            G M+  D +    +K+ ++ I    LD    QLFW S AN WCT+   ++ +  +K   H L   RV++ FSN+ +F +DF CP+ S MN   KC++
Sbjct:  254 GAMYVRDFFKESSKKNIKDMIHSIRLD---NQLFWLSRANMWCTKIRAKSEEMLLKTNPHSLPIARVDLSFSNNEDFVRDFNCPVDSKMNLSKKCRV 347          

BLAST of neprilysin-2-like isoform x3 vs. L. salmonis genes
Match: EMLSAG00000000398 (supercontig:LSalAtl2s:LSalAtl2s1063:30854:35288:1 gene:EMLSAG00000000398 transcript:EMLSAT00000000398 description:"maker-LSalAtl2s1063-augustus-gene-0.34")

HSP 1 Score: 103.219 bits (256), Expect = 9.975e-23
Identity = 99/440 (22.50%), Postives = 178/440 (40.45%), Query Frame = 0

Query:   85 RLR-GGP-EICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRS--SVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLA---FFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNK-MVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAK-----DKVSEMVA-LVTEAFRIRLIQGKSYAAAAKKGFLVKVGTGDI 510
            RLR  GP   C TP+C+ AA  ++ + + +  PC +F+ Y+C  +     IP  +S  SV E +    +   E +     T   E S+   +      F+ +CM    +E+   +PL   +   GGW +          S   ++  H      F  + LF + V  D ++   NI  +    LG+  K       +   V AY  Y  D A+L G       K ++  M  FE  ++  T S   +SD   + N MT+ +L      + W++ +       S   +            ++V+ P+Y   + + + +T   ++ +Y++WR   T +PY++   R V   + +  SG +    RW+ C   T  FF   +   Y +    P++      D +   VA  V+ +      + +       K   +++GT D 
Sbjct:    4 RLRHSGPLSYCTTPSCLQAAASILASQNASYSPCSEFWQYACGSWLSTHSIPASKSYWSVLEEAKAGSR---EDKSRLITTSSHEPSQFNSLEWKVSNFYHSCMSLSFIESDKEKPLLKIINSLGGWHVLRSFNLYSWDSRRVLRELHS----EFAVHALFRIGVVPDVQDPDYNIIAIYPDGLGMSDKSYYHRLPDDPAVVAYQTYLKDSAMLFGASSPEAHKFSI-DMFNFEKRISEITPSGEYLSDPRKINNKMTVKDLHTMSMTVPWLEILKAAFSDASTTEET----------GIIVVSPQYVADIAVIMSTTDRGSLNNYLMWRLXXTYMPYLSKNYREVVDVYRKSISGAQKPLERWEFCQETTDRFFGHYLNAIYSQRDNIPDSVVKKVFDYIKHTVAKTVSVSSHFEDYESRRATVGKLKNMSIQIGTPDF 425          

HSP 2 Score: 52.373 bits (124), Expect = 4.920e-7
Identity = 48/182 (26.37%), Postives = 78/182 (42.86%), Query Frame = 0

Query:  648 DKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNAL-------SRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHG-------FDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSN 815
            D E+    + KL +++  IG  D + +   +  LY+DL +    FFQN L        R +  L  S   ++Q  +E      +Q + +   Y P  N + +P   L+  F +   PN +N G IG  +   + +G       FD  G+  DEE N  N   NK +      A+  +  YS+
Sbjct:  402 DYESRRATVGKLKNMSIQIGTPDFLRDRKNLKTLYKDLLVQTNDFFQNILYGVSFLRKRQEALLLHSSESVSQLENEYWLESLNQESEI--VYVPSRNKVVVPELFLRSPFVDPGYPNSINMGGIGVRLTEAVINGIIGIGLYFDSKGRFVDEENN-NNKAHNKQSLWIEMSAERLMKSYSS 580          

BLAST of neprilysin-2-like isoform x3 vs. L. salmonis genes
Match: EMLSAG00000007067 (supercontig:LSalAtl2s:LSalAtl2s3:2246372:2348867:-1 gene:EMLSAG00000007067 transcript:EMLSAT00000007067 description:"maker-LSalAtl2s3-augustus-gene-22.10")

HSP 1 Score: 98.5969 bits (244), Expect = 1.170e-21
Identity = 66/234 (28.21%), Postives = 107/234 (45.73%), Query Frame = 0

Query:  710 QLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQY-SNFTVP------ELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESI-LPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            +LN+ T  DP         VN   +    S+ +P+GIL+   +  N P YLN GS+G  + HE+ H  D+ G+ F+ +G  +NWW     + F   + C   QY  +F  P       + + ++G     E I D  G+    D      + + ++ + LP       QLF+ + A  +C+     +    ++   H     RV+    N+  F+  FKCPLGS MNP+ KC +
Sbjct:  289 KLNEITWLDPKTLILTKDKVNRIQASFYGSM-IPSGILKEIHFKSNIPMYLNLGSLGLTLSHEIFHSLDEMGRGFNSQGVFENWWTPSDEKSFSNVSHCIKRQYVEHFRRPLKIDTRSILIEVDGAFTLNENICDVDGMNIVSDVLKDMSKNNFQDVVHLPNNPYPPVQLFFINIAQAYCSHIGPVSYILYLELDEHSPNPERVDGFMMNAELFSNAFKCPLGSRMNPKRKCSV 521          

BLAST of neprilysin-2-like isoform x3 vs. L. salmonis genes
Match: EMLSAG00000003459 (supercontig:LSalAtl2s:LSalAtl2s189:451654:451974:1 gene:EMLSAG00000003459 transcript:EMLSAT00000003459 description:"augustus_masked-LSalAtl2s189-processed-gene-4.0")

HSP 1 Score: 81.6481 bits (200), Expect = 8.313e-19
Identity = 45/109 (41.28%), Postives = 67/109 (61.47%), Query Frame = 0

Query:  742 LPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTV--PELNLTIEGKHIQGEGIADNGGIMHAYDAY 848
            +P  ILQ   +   +P  L +G+ G I+ H++TH FDDSG ++DE G  K  +++KT + FR ++DCF  QYS+F +  PE    I+G    GE +AD+GG+  A  AY
Sbjct:    3 VPIAILQPPSF-WTKPQSLAFGAFGIIVAHKITHAFDDSGIKYDEYGFYKQLYDDKTVKAFRKESDCFRQQYSSFQLSGPE----IDGNRTLGENVADHGGLKIAEIAY 106          

BLAST of neprilysin-2-like isoform x3 vs. L. salmonis genes
Match: EMLSAG00000009820 (supercontig:LSalAtl2s:LSalAtl2s639:498865:531508:1 gene:EMLSAG00000009820 transcript:EMLSAT00000009820 description:"snap_masked-LSalAtl2s639-processed-gene-5.6")

HSP 1 Score: 77.411 bits (189), Expect = 2.458e-17
Identity = 40/98 (40.82%), Postives = 56/98 (57.14%), Query Frame = 0

Query:  838 NGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            N G  +  +  SA      +E +LPGL+ T +QL+W S+AN WC+  N E+I +N  +  H   KYRV  P SN  EF+K F C   +PMN   KC++
Sbjct:    6 NSGCDNRKEIVSAIKNDDHDEDLLPGLNYTQEQLYWISSANLWCSVYNPEHI-FNEIFDDHSPNKYRVIGPLSNRPEFSKAFHCAANTPMNRTEKCQM 102          

BLAST of neprilysin-2-like isoform x3 vs. L. salmonis genes
Match: EMLSAG00000004383 (supercontig:LSalAtl2s:LSalAtl2s2301:992:4937:1 gene:EMLSAG00000004383 transcript:EMLSAT00000004383 description:"maker-LSalAtl2s2301-snap-gene-0.9")

HSP 1 Score: 75.0998 bits (183), Expect = 2.394e-14
Identity = 55/212 (25.94%), Postives = 93/212 (43.87%), Query Frame = 0

Query:  646 WMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQ----GKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVE 853
            WM       A+ KL  +   +GY   +LN T M+ L++ + ++   F  ++LS   +      N +   + +  W            +   +   N+ AGIL       FY+   P YLN+G++G II HE++H FD  G   + +G    W++    + +  K  C + +Y   ++  +N T    H+  E I D  G    Y+AYS   E
Sbjct:  177 WMSPSVKKYAMAKLLRMKIFVGYPKEMLNDTLMEGLHQRIKIYS-DFLSSSLSIEAY----HQNDIGPHSRKLDW------------FMHFNGLDNVKAGILNEHSNTHFYSLKHPMYLNFGALGSIISHEISHSFDQMGICINGKGKYSEWFDMDATQYYSQKTRCILQKYK--SILNMNWT----HMANENIVDILGFQTTYEAYSKKKE 365          

BLAST of neprilysin-2-like isoform x3 vs. L. salmonis genes
Match: EMLSAG00000001418 (supercontig:LSalAtl2s:LSalAtl2s1243:89570:96753:-1 gene:EMLSAG00000001418 transcript:EMLSAT00000001418 description:"maker-LSalAtl2s1243-augustus-gene-0.5")

HSP 1 Score: 62.3882 bits (150), Expect = 1.151e-10
Identity = 36/117 (30.77%), Postives = 60/117 (51.28%), Query Frame = 0

Query:   91 EICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLG-LQPLRDFLKIPGGWPL 206
            ++C + +CV AA  ++ ++D ++DPCEDFY Y+C G+  R +I     +     ++  + Q  +  +  E    E    AK+   ++Q C+   E  NL  LQ L D+    GGW L
Sbjct:   41 KVCLSEDCVKAAATILFSLDRSVDPCEDFYQYACGGWATRTII----QNTDRFQMVDKRNQLFIHNLLTEMNENEDEAVAKVK-KYYQVCISEDEKRNLPYLQSLIDY---TGGWTL 149          

BLAST of neprilysin-2-like isoform x3 vs. SwissProt
Match: gi|1270743871|sp|A0A0B4K692.1|NEP2_DROME (RecName: Full=Neprilysin-2; Contains: RecName: Full=Neprilysin-2, soluble form)

HSP 1 Score: 299.671 bits (766), Expect = 1.403e-87
Identity = 136/292 (46.58%), Postives = 191/292 (65.41%), Query Frame = 0

Query:  644 LDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            ++WMD +T   A +KL+S+   IGY D +L+   +   Y  LD+ P ++F++ L  + +    S+N+L    ++  W    + AIVNAFYS L NSI  PAGILQG F+N  RP Y+N+G+IGY+IGHE+THGFDD G+QFD +GNL++WW   T + +  KA C ++QY N+T     L + G + QGE IADNGG+  +Y AY  W EKHG E+ LPGLD T +Q+FW +A  TWC +   E++K  +  GVH  +++RV    SN ++FAKDF CP GSPMNP  KC++
Sbjct:  482 VNWMDAKTKKEAKLKLHSMATHIGYPDEMLDNEKLAAYYAKLDIDPDKYFESFLGMNIFGTDYSFNKLRLPVNKTDWVRHARPAIVNAFYSSLENSIQFPAGILQGHFFNAQRPKYMNFGAIGYVIGHEITHGFDDQGRQFDVKGNLRDWWHPDTQKAYLAKAKCIIEQYGNYTERATGLNLNGINTQGENIADNGGVKESYIAYRRWAEKHGPEAKLPGLDYTPEQMFWVAAGQTWCAKYRKESLKMRITTGVHSPSEFRVLGSLSNMKDFAKDFHCPEGSPMNPVQKCEV 773          

HSP 2 Score: 205.297 bits (521), Expect = 3.912e-54
Identity = 122/406 (30.05%), Postives = 198/406 (48.77%), Query Frame = 0

Query:   91 EICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDL-KSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLD-PEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCL-TATSNF----------FPLLVGKFYVESHFPPEAKDKVSEMVALVTEAF 483
            ++C T  C+  A  +++ M   ++PC++FY ++C  + +   IPDD+ S+S  S+++D++Q +L+ I     P    +  ++P   ++ACM+   +E LG +P+    +  GGWPL          S TW +   K    GF+ +Y+    +G D +N+T  +  L Q  L L  + L +G+   +V AYY Y  D+AVL G  +  DL K+ L   ++FE+ LAN +       +   LYNL T  +L   YP++ WV Y+N +      VA+           E++ L  P +FE L   L  TP   I +Y+ WR    SV +++ + R    Q+    SG +    RWK C+  ATS+             + VG  YV  HF  ++K    EMV  +   F
Sbjct:   82 DVCLTQECIHTASTVLRKMKPEVEPCDNFYEFACGTYLEEENIPDDKVSISTFSVISDKLQEQLKDIITAERPETEPKHFRLPNLLYKACMNKTLIETLGPEPITRVAERLGGWPLIKGDSWNADDSWTWQEQVKKFRTAGFSMDYIIDFSIGVDLQNSTKRLIDLDQSSLALSREYLVKGFNETLVTAYYKYMVDIAVLFGANR--DLAKTELLLSLEFEMALANISWPNEKRRNSSELYNLRTPAQLQAAYPYVQWVDYMNALLPEGLNVAE----------DEMINLSVPSFFEDLGKLLAKTPKRVIANYMFWRIHGFSVGFLSEEFRKRQLQYATALSGRQEQEARWKECVDIATSSMDEVCEDDFDSLGISVGSLYVGKHFHKDSKANALEMVNEIRNVF 475          

BLAST of neprilysin-2-like isoform x3 vs. SwissProt
Match: gi|74949025|sp|Q9W436.2|NEP_DROME (RecName: Full=Neprilysin-1)

HSP 1 Score: 278.485 bits (711), Expect = 3.683e-79
Identity = 132/291 (45.36%), Postives = 179/291 (61.51%), Query Frame = 0

Query:  645 DWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            DWMD ET + A  K +S+NE IGY +++ N T ++  Y +L + P  F  N LS  +W  +     L Q   ++ W    + A+VNAFY+P  N I  PAGILQ  FY+++ P  LNYG IG +IGHE+THGFDD G+QFD+EGN+  WW N T E FR +  C +DQYS + + E+++ ++G+  QGE IADNGG+  A+ AY  W   HG E  LPGL++T  QLF+ + A  WC     E+    ++  VH     RV  P SNSR+FA  +KCPLGS MNP  KC +
Sbjct:  560 DWMDDETRAVAKEKADSMNERIGYPELLTNATELEQEYVNLTIVPDNFINNVLSILQWESEKMLRLLRQPVDKEKWT--TEPAVVNAFYNPNKNDIVFPAGILQPLFYSQHFPKSLNYGGIGVVIGHEITHGFDDKGRQFDKEGNMMQWWNNATIEAFRERTQCVIDQYSRYKINEVDMFMDGRMTQGENIADNGGLKQAFRAYKKWETLHGREQQLPGLNMTHDQLFFLNYAQIWCGSMRPEDALTKIRSAVHSPGFVRVLGPLSNSRDFASAYKCPLGSTMNPAEKCSV 848          

HSP 2 Score: 181.415 bits (459), Expect = 6.564e-46
Identity = 116/412 (28.16%), Postives = 196/412 (47.57%), Query Frame = 0

Query:   75 DGAIEDELGIRLRGGPEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKE--LKEGWKNKMVQAYYNYTYDMAVLLG-EPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAF 483
            +G    + G    G   IC T  CV  A  L+  MDL  DPCEDF+ Y+C  + K   IP+DRSS+S   +L+DQ Q  LR + +E +    ++       FF++CMD  ++  +G   L+  L+  GGWP+    R     +   V+     + + ++   +  L VG D KN++ NI  + Q +  L  ++  LKE   N   +AY+ Y   +A+LLG +P T    + L K++ FE  L N +   AD  D   +Y  M L EL +  P + W +Y+          A +     +Q ++ +V     Y   +   L  T    + +Y++WR + + + +M  + +    +F +   G +S+  RW  C+  T+    + VG  ++  +F  E+K+   EM+  +  AF
Sbjct:  155 NGRWNQDSGSAHEGQDNICMTQECVRTAASLLSAMDLNSDPCEDFFQYACGTWNKMHPIPEDRSSISTFEVLSDQQQVILRAVLEEPIDERDNKATIKAKTFFKSCMDIPQIRKIGTGRLKQVLQSLGGWPV--IERNWSPPADLSVERLMGQLRLNYSEPVMIELYVGADDKNSSVNILQMDQLQYALPSRDYYLKESSANDR-RAYHRYMTQVALLLGADPATA--AAELEKVVLFETQLVNVSLPEADRHDTSLVYRKMLLPELQELVPEVQWQEYLQ---------AALGPGIPLQEDEPLVTYGLHYLTEMGKILAHTDRRVVHNYMLWRLVMSLMSHMIDEYQRERVEFRKILMGIQSERTRWSQCVEWTNKKLGVAVGALFIRDNFNQESKEVALEMIHTIRAAF 552          

BLAST of neprilysin-2-like isoform x3 vs. SwissProt
Match: gi|74930356|sp|Q8IS64.1|ECE_LOCMI (RecName: Full=Endothelin-converting enzyme homolog; Short=ECE)

HSP 1 Score: 268.47 bits (685), Expect = 1.455e-76
Identity = 130/292 (44.52%), Postives = 184/292 (63.01%), Query Frame = 0

Query:  644 LDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            L WMD ET   A  K ++I+++IG+ D ILN   +D  Y+DL++   ++F+N L  +K+ LK +  +L+Q  ++  W        VNA+Y+P  N I  PAGILQ  FY+   P  +NYG++G ++GHELTH FDD G+++D+ GNL  WW N+T E F+ +  C VDQYSN+TV   N  + GK   GE IADNGG+  AY AY  W +++  E  LPGL+ + KQLF+ S A  WC+ S  E IK  ++   H   K+RV  P SN +EF+ +F+CPLGS MNP  KC++
Sbjct:  439 LKWMDAETRRAAEKKADAISDMIGFPDYILNPEELDKKYKDLEIKEDEYFENNLRVNKYNLKSNLEKLDQPVNKTRWGM--TPPTVNAYYTPTKNQIVFPAGILQAPFYDIGHPKSMNYGAMGVVMGHELTHAFDDQGREYDQNGNLHKWWNNQTIEAFKKRTQCVVDQYSNYTVD--NKHVNGKQTLGENIADNGGLKAAYHAYLEWEQRNPRELPLPGLNFSHKQLFFLSFAQVWCSASTDEAIKLQLEKDPHAPPKFRVIGPLSNLQEFSTEFRCPLGSKMNPVHKCEV 726          

HSP 2 Score: 162.54 bits (410), Expect = 3.504e-40
Identity = 111/450 (24.67%), Postives = 206/450 (45.78%), Query Frame = 0

Query:   64 LLAVFVVNTVLDGAIEDELGIRLRGGPEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKE-VENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKE--LKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTT-----IQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFR-----IRLIQGKSYAAAAKKG 500
            LLA+ +V+ VL           L      C + +CV AA  ++  MD T DPC DFY Y+C G+ +   IPD +S       L  Q Q  ++ + ++ +    S   +    ++ +C+D  + +E LG +P+ D L   GGW +      K  +    ++      ++G     LF   VG D +++  +I  + Q  L L  ++  L E   +K++ AY  Y   + VLLG  +  ++++ +  +I+FE  LA       D  D + LYN M L ++  + PF++W  + +    + +         +I   ++VVV  PEY E+L   +++  +TT     + +Y++W+ +++   Y++   R       +   G+      W+ C+T T+N     +G  +V   F   +K     M+  +  AF+     ++ +  ++  AA KK 
Sbjct:   20 LLALLIVSAVL--LSRPYAPALLHAEEAYCVSMSCVTAAASVLSLMDATADPCSDFYQYACGGWVRANPIPDTKSMWGTFVKLEQQNQLVIKNVLEQPMSEFKSEAERKAKLYYMSCLDVNDTIETLGPKPMLDLLVKIGGWNITGNFSIKNWSLQKSLETLQNRYNMGG----LFTWAVGEDDRDSKKHIIQIDQGGLTLPTRDNYLNETMDDKVLSAYLEYMTKIGVLLG-GEEKNVRTQMKAVIEFETELAKIMSPQEDRRDEEKLYNNMELDKVQGRPPFINWHAFFSNAMENITR--------KISKKEKVVVYAPEYLEKLNDIIRNYTNTTDGKIILNNYLVWQTVRSMTSYLSKAFRDAYKGLRKALVGSEGGEKPWRYCVTDTNNVIGFAIGAMFVREAFHGNSKPAAENMINQIRTAFKSNLKNLKWMDAETRRAAEKKA 454          

BLAST of neprilysin-2-like isoform x3 vs. SwissProt
Match: gi|1706564|sp|P42893.2|ECE1_RAT (RecName: Full=Endothelin-converting enzyme 1; Short=ECE-1)

HSP 1 Score: 261.922 bits (668), Expect = 6.792e-74
Identity = 124/292 (42.47%), Postives = 183/292 (62.67%), Query Frame = 0

Query:  644 LDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            L WMD++T   A  K ++I  +IGY + I++   +D ++ D    P  +F+NA+    + L+ + +QL ++ + D W       +VNA+YSP  N I  PAGILQ  FY  + PN LN+G IG ++GHELTH FDD G+++D++GNL+ WW+N + E F+ + +C V QY+N++V      + G+H  GE IADNGG+  AY AY  WV+K+G E ILP L LTS QLF+   A  WC+    E+    +    H  +++RV    SNS+EF++ F+CPLGSPMNP  KC++
Sbjct:  474 LKWMDEDTRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSLRVTADQLRKAPNRDQWSM--TPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYNNYSVN--GEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQILPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPLGSPMNPRHKCEV 761          

HSP 2 Score: 173.711 bits (439), Expect = 1.067e-43
Identity = 111/434 (25.58%), Postives = 207/434 (47.70%), Query Frame = 0

Query:   58 LVTISALLAVFVVNTVLDGAIEDELGIRLRG-GPEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKE--LKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDS-PRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFRIRL 487
            LV +  LLA  +V      A    LGI+ R   P +C T  CV     ++ +MD T+DPC+DF++Y+C G+ K   +PD  S     S L +  Q  ++ + + +    +S   K    +++ACM+   +E L  +PL + ++  GGW +           T  V  AH      +  +  F + V  D KN+ +N+  + Q  LGL  ++  L +    K++  Y NY   +  LLG      ++  + +++ FE  LAN T       D + +Y+ +T  EL    P ++W+ ++N +          +   +I  ++ +VV D EY  +++  + ST    + +Y++W  ++ +  +++ + +    +F     GT+    PRWK C++ T N     +G  +V++ F  ++K+  SE++  + +AF   L
Sbjct:   61 LVVLVTLLAAGLV------ACLAALGIQYRTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTA-SASEAEKKAQVYYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAH------YRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDSIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNAI----------FYPVEINESEPIVVYDKEYLRQVSTLINSTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESL 471          

BLAST of neprilysin-2-like isoform x3 vs. SwissProt
Match: gi|81918086|sp|Q4PZA2.1|ECE1_MOUSE (RecName: Full=Endothelin-converting enzyme 1; Short=ECE-1)

HSP 1 Score: 256.914 bits (655), Expect = 4.980e-72
Identity = 123/292 (42.12%), Postives = 180/292 (61.64%), Query Frame = 0

Query:  644 LDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            L WMD+ET   A  K ++I  +IGY + I++   +D ++ D    P  +F+NA+    +  + + +QL ++ + D W       +VNA+YSP  N I  PAGILQ  FY  + PN LN+G IG ++GHELTH FDD G+++D++GNL+ WW+N + E F+ + +C V QYSN++V      + G+H  GE IADNGG+  AY AY  WV+K+G E  LP L LTS QLF+   A  WC+    E+    +    H  +++RV    SNS+EF++ F+CP GSPMNP  KC++
Sbjct:  481 LKWMDEETRRSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSM--TPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVQQYSNYSVN--GEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTSNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPHHKCEV 768          

HSP 2 Score: 169.474 bits (428), Expect = 3.035e-42
Identity = 110/436 (25.23%), Postives = 209/436 (47.94%), Query Frame = 0

Query:   58 LVTISALLAVFVVNTVLDGAIEDELGIRLRG-GPEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLP--LESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKE--LKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDS-PRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFRIRL 487
            LV +  LLA  +V      A    LGI+ +   P +C T  CV     ++ +MD T+DPC+DF++Y+C G+ K   +PD  S     S L +  Q  ++ + +       E+ R A++   +++ACM+   +E L  +PL + ++  GGW +           T  V  AH      +  +  F + V  D KN+ +N+  + Q  LGL  ++  L +    K++  Y NY   +  LLG      ++  + +++ FE  LAN T       D + +Y+ +T  EL    P ++W+ ++N +          +   +I  ++ +VV D EY  +++  + +T    + +Y++W  ++ +  +++ + +    +F     GT+    PRWK C++ T N     +G  +V++ F  ++K+  SE++  + +AF   L
Sbjct:   68 LVVLVTLLAAGLV------ACLAALGIQYQTRTPPVCLTEACVSVTSSILNSMDPTVDPCQDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAVIKHLLENATASVSEAERKAQV---YYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAH------YRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEDAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTI----------FYPVEINESEPIVVYDKEYLRQVSTLINNTDKCLLNNYMMWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCIPRWKFCVSDTENNLGFALGPMFVKATFAEDSKNIASEIIMEIKKAFEESL 478          

BLAST of neprilysin-2-like isoform x3 vs. SwissProt
Match: gi|1706563|sp|P42892.2|ECE1_HUMAN (RecName: Full=Endothelin-converting enzyme 1; Short=ECE-1)

HSP 1 Score: 252.677 bits (644), Expect = 1.524e-70
Identity = 121/292 (41.44%), Postives = 180/292 (61.64%), Query Frame = 0

Query:  644 LDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            L WMD+ET   A  K ++I  +IGY + I++   +D ++ D    P  +F+NA+    +  + + +QL ++ + D W       +VNA+YSP  N I  PAGILQ  FY  + P  LN+G IG ++GHELTH FDD G+++D++GNL+ WW+N + E F+ + +C V+QYSN++V      + G+H  GE IADNGG+  AY AY  WV+K+G E  LP L LT+ QLF+   A  WC+    E+    +    H  +++RV    SNS+EF++ F+CP GSPMNP  KC++
Sbjct:  482 LKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSM--TPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVN--GEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEV 769          

HSP 2 Score: 168.703 bits (426), Expect = 5.059e-42
Identity = 103/412 (25.00%), Postives = 200/412 (48.54%), Query Frame = 0

Query:   82 LGIRLRG-GPEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLP--LESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKE--LKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDS-PRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFRIRL 487
            LGI+ +   P +C +  CV     ++ +MD T+DPC DF++Y+C G+ K   +PD  S     S L +  Q  ++ + + +     E+ R A++   +++ACM+   +E L  +PL + ++  GGW +           T  V  AH      +  +  F + V  D KN+ +N+  + Q  LGL  ++  L +    K++  Y NY   +  LLG      ++  + +++ FE  LAN T       D + +Y+ +T  EL    P ++W+ ++N +          +   +I  ++ +VV D EY E+++  + +T    + +Y+IW  ++ +  +++ + +    +F     GT+    PRWK C++ T N     +G  +V++ F  ++K   +E++  + +AF   L
Sbjct:   87 LGIQYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQV---YYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAH------YRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTI----------FYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESL 479          

BLAST of neprilysin-2-like isoform x3 vs. SwissProt
Match: gi|74933939|sp|Q8T062.1|NEP4_DROME (RecName: Full=Neprilysin-4)

HSP 1 Score: 256.529 bits (654), Expect = 2.109e-70
Identity = 123/308 (39.94%), Postives = 184/308 (59.74%), Query Frame = 0

Query:  631 HPHSKDYKLLTGMLDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEE---SILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            H   + ++ +    DW+D  T   A  K+N+++  IGY D ILN + ++  Y  ++++P+++F+N L+      K    +L++  ++  W      AIVNA+YS   N I  PAGILQ  FY+ + P  LN+G IG +IGHELTHGFDD G+ FD  GN+  WW + +   F  +A C + QYSN+TV E+ + + G+  QGE IADNGG+  A+ AY  W+++H  E    ILPGL++T  QLF+ +    WC     E I+  +   +H   ++RV    SNS +FA++F CPLGSPMNP+ KC +
Sbjct:  734 HDLQQAFRDILKTTDWLDDTTKQLAEEKVNAMSLKIGYPDFILNPSELNSKYAGIEIYPEKYFENTLNVLLHTAKTEQAKLHERVNKTNWQ--TAPAIVNAYYSRNKNQIMFPAGILQPPFYHRHFPKSLNFGGIGVVIGHELTHGFDDKGRLFDRNGNIHKWWTDSSIRGFDERARCIIAQYSNYTVEEVGIVLNGESTQGENIADNGGLRQAFHAYQRWLKEHPSEVSDEILPGLNMTGPQLFFLNFGQVWCGAMRPEAIRNKLNTAIHSPGRFRVIGTLSNSVDFAREFNCPLGSPMNPQKKCSV 1039          

HSP 2 Score: 114.005 bits (284), Expect = 2.009e-24
Identity = 79/310 (25.48%), Postives = 135/310 (43.55%), Query Frame = 0

Query:  176 FFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKE-LKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFR 484
             +++C++S  +   GL+PL   ++  GGWP+     ++  ++  W  LA  L    +  + L    VG D KN+  NI    Q  LGL  +E   +    K +QAY  Y  ++   +G  K  D +   ++++ FE  LA  T       +V  LY  MTL +L    P + W  Y+           Q     ++   +EVV+   EY  +L   L  T   T+ +Y++WR ++  +  ++ +   +   FY    G      RWK C+   +    + VG  +V  +F   +K     M   + +AFR
Sbjct:  448 LYRSCVNSAVLAKRGLEPLHTLIRELGGWPVLESQWSE--SNFNWQVLAATLRR--YNNDILIVQWVGADIKNSEENIVQFDQTGLGLPTREYFLQPSNAKYLQAYQRYMAEVMHKMGASKA-DAQRVASELVAFETQLAGITAPAEQRLNVTKLYKRMTLDQLQAVVPEIKWRAYL-----------QSLQDREVLGTEEVVIYAVEYMSKLVTLLDETDPRTVSNYMMWRFVRHRINNVDDRFDDIKQSFYHALFGREESPQRWKVCIAQVNTNMGMAVGSMFVSRYFDNNSKRDTLRMTHDLQQAFR 741          

BLAST of neprilysin-2-like isoform x3 vs. SwissProt
Match: gi|190359620|sp|P42891.2|ECE1_BOVIN (RecName: Full=Endothelin-converting enzyme 1; Short=ECE-1)

HSP 1 Score: 251.521 bits (641), Expect = 3.263e-70
Identity = 121/292 (41.44%), Postives = 179/292 (61.30%), Query Frame = 0

Query:  644 LDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            L WMD++T   A  K ++I  +IGY + I++   +D ++ D    P  +F+NA+    +  + + +QL ++ + D W       +VNA+YSP  N I  PAGILQ  FY  + PN LN+G IG ++GHELTH FDD G+++D++GNL+ WW+N + E F+ +  C V+QY N++V      + G+H  GE IADNGG+  AY AY  WV+K+G E  LP L LT+ QLF+ S A  WC+    E+    +    H  +++RV    SNS+EF++ F CP GSPMNP  KC++
Sbjct:  466 LKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSM--TPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPNALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTACMVEQYGNYSVN--GEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEQTLPTLGLTNNQLFFLSFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSISNSKEFSEHFHCPPGSPMNPHHKCEV 753          

HSP 2 Score: 166.777 bits (421), Expect = 2.009e-41
Identity = 98/403 (24.32%), Postives = 195/403 (48.39%), Query Frame = 0

Query:   90 PEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLP--LESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKE--LKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDS-PRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFRIRL 487
            P +C +  C+     ++ +MD T+DPC+DF+ Y+C G+ K   +PD  S     S L +  Q  ++ + + +     E+ R A++   +++ACM+   +E L  +PL + ++  GGW +           T  V  +H      +  +  F + V  D KN+ +N+  + Q  LGL  ++  L +    K++  Y NY   +  LLG      ++  + +++ FE  LAN T       D + +Y+ +T  EL    P ++W+ ++N +          +   +I  ++ +V+ D EY  +++  + ST    + +Y+IW  ++ +  +++ + +    +F     GT+    PRWK C++ T N     +G  +V++ F  ++K+  SE++  + +AF   L
Sbjct:   80 PSVCLSEACISVTSSILSSMDPTVDPCQDFFTYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQV---YYRACMNETRIEELKAKPLMELIEKLGGWNITGPWDKDNFQDTLQVVTSH------YHTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGAEDTIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTI----------FYPVEINESEPIVIYDKEYLSKVSTLINSTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENTLGFALGPMFVKATFAEDSKNIASEIILEIKKAFEESL 463          

BLAST of neprilysin-2-like isoform x3 vs. SwissProt
Match: gi|1352911972|sp|P0DPD6.1|ECE2_HUMAN (RecName: Full=Endothelin-converting enzyme 2; Short=ECE-2)

HSP 1 Score: 250.366 bits (638), Expect = 1.886e-69
Identity = 125/294 (42.52%), Postives = 172/294 (58.50%), Query Frame = 0

Query:  642 GMLDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            G L WMD++T   A  K ++I ++IG+ D IL    +D +Y   ++    FFQN L+   +  K   +QL +  S D W    Q   VNA+Y P  N I  PAGILQ  FY  N P  LN+G IG ++GHELTH FDD G+++D+EGNL+ WW+N++   FR    C  +QY+ + V    L   G+   GE IADNGG+  AY+AY AW+ KHGEE  LP + LT+ QLF+   A  WC+    E+    +    H  A++RV    SNSR+F + F CP+GSPMNP   C++
Sbjct:  521 GQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQT--VNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLN--GRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEV 810          

HSP 2 Score: 162.925 bits (411), Expect = 5.375e-40
Identity = 103/407 (25.31%), Postives = 201/407 (49.39%), Query Frame = 0

Query:   93 CQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPL-GPKMRTKKGTSTTWVQLAHKLMDV--GFTGNY----LFGLDVGTDFKNTTNNIFFLAQPRLGLEYKE--LKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGT-RSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFRIRLIQ 489
            C T  C+  A ++++++D  + PCEDFY +SC G+ +R  +PD RS  +  + L DQ Q  L+ + + T    SS   +    F+ +C+  + +E LG QPLRD ++  GGW + GP           W Q     M+V     G Y     F + +  D K++ +N+  + Q  L L  ++  L      K++ AY +Y  ++ +LLG  + T  +  + ++++ EI LAN T       D + +Y+ M++ EL    P + W+++          ++ + S  ++  ++ VVV   +Y ++++  +  T  + + +Y+IW  ++ +   ++ +  +   +      GT +S  PRW+ C++ T +     +G  +V++ F  ++K+    M++ +  AF   L Q
Sbjct:  140 CLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAEQKTQRFYLSCLQVERIEELGAQPLRDLIEKIGGWNITGP-----------WDQ--DNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGMLLG-GRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEF----------LSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQ 522          

BLAST of neprilysin-2-like isoform x3 vs. SwissProt
Match: gi|2499916|sp|P97739.1|ECE1_CAVPO (RecName: Full=Endothelin-converting enzyme 1; Short=ECE-1)

HSP 1 Score: 248.054 bits (632), Expect = 4.303e-69
Identity = 119/292 (40.75%), Postives = 178/292 (60.96%), Query Frame = 0

Query:  644 LDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            L WMD++T   A  K ++I  +IGY + I++   +D ++ D    P  +F+NA+    +  + +  QL ++ + D W       +VNA+YSP  N I  PAGIL   FY  + P  LN+G IG ++GHELTH FDD G+++D++GNL+ WW+N + E F+ + +C V+QYSN++V      + G+H  GE IADNGG+  AY AY  WV+K+G E  LP L LT+ QLF+   A  WC+    E+    +    H  +++RV    SNS+EF++ F+CP GSPMNP  KC++
Sbjct:  466 LKWMDEDTRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTAEQLRKAPNRDQWSM--TPPMVNAYYSPTKNEIVFPAGILPAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKQQTECMVEQYSNYSVN--GEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEETLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFQCPPGSPMNPRHKCEV 753          

HSP 2 Score: 174.481 bits (441), Expect = 5.757e-44
Identity = 111/436 (25.46%), Postives = 212/436 (48.62%), Query Frame = 0

Query:   58 LVTISALLAVFVVNTVLDGAIEDELGIRLRG-GPEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLP--LESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKE--LKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDS-PRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFRIRL 487
            L+ + ALLA  +V      A    LGI+ R   P +C +  CV     ++ +M+ T+DPC+DF++Y+C G+ K   +PD  S     S L +  Q  ++ + + +     E+ R A++   +++ACM+   +E L  +PL + ++  GGW +           T  V  AH      +  +  F + V  D KN+  N+  + Q  LGL  ++  L +    K++  Y NY   +  LLG      +++ + +++ FE  LAN T       D + +Y+ +T  EL    P ++W+ ++N +          +   +I  ++ +VV D EY E+++  + +T    + +Y+IW  ++ +  +++ + +    +F     GT+    PRWK C++ T N     +G  +V++ F  ++K+  SE++  + +AF   L
Sbjct:   53 LIVLVALLAAGLV------ACLTALGIQYRTRTPPVCLSEACVSVTSSILNSMNPTVDPCQDFFSYACGGWIKANPVPDGHSRWGAFSNLWEHNQAIIKHLLENSTASVSEAERKAQV---YYRACMNETRIEELRAKPLMELIEKLGGWNITGPWAKDNFQDTLQVVTAH------YRTSPFFSVYVSADSKNSNRNVIHVDQSGLGLPSRDYYLNKTENEKVLNGYLNYMVQLGKLLGGGDENAIRAQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTI----------FYPVEINESEPIVVYDKEYLEQVSTLINTTDKCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFGLGPMFVKATFAEDSKNIASEIILEIKKAFEESL 463          

BLAST of neprilysin-2-like isoform x3 vs. nr
Match: gi|1325273790|ref|XP_023321543.1| (neprilysin-2-like [Eurytemora affinis])

HSP 1 Score: 334.339 bits (856), Expect = 5.673e-98
Identity = 152/293 (51.88%), Postives = 201/293 (68.60%), Query Frame = 0

Query:  644 LDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLD-LTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            +DWMD++T  RA  KL S+ E IGY + IL    ++ LY +L+++P+ +F N +  S W    SW +L +   +  W      A+VNAFYS + NSI  PAGILQG F+N +RP Y+NYG+IG++IGHE+THGFDD G+Q+D+EGNLKNWWE+ T + F  KA+C V QY N+T   +N T+ G + QGE IADNGGI  AY AY +WV +HGEE  LPGL   T KQ+FW SAANTWC++   + ++  +K G H    +RV  PFSNSREFA+DF CPLGS MNP  KC++
Sbjct:  473 IDWMDEKTRERAKAKLKSMKEYIGYPEEILENWRLEELYENLEINPETYFMNGIKMSIWGTNYSWGKLREKVDKTDWKRHGAPAVVNAFYSSIENSIQFPAGILQGNFFNADRPAYMNYGAIGWVIGHEITHGFDDQGRQYDDEGNLKNWWEDSTKDRFLEKANCIVWQYGNYTADSVNKTLNGVNTQGENIADNGGIKEAYRAYQSWVHRHGEEHKLPGLHGYTGKQMFWISAANTWCSKYRDKALEKRIKTGAHSPGMFRVKGPFSNSREFAEDFNCPLGSNMNPVDKCEV 765          

HSP 2 Score: 249.98 bits (637), Expect = 3.256e-67
Identity = 128/393 (32.57%), Postives = 217/393 (55.22%), Query Frame = 0

Query:   91 EICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAF 483
            ++C +  C+  +  ++ NMD T+DPC+DFY ++C GF+ R++I DDRSS S+ S++ D +  +LR+I +       SR   +    ++ACMD + +E +GLQPL+D LK  GGWP+  +   +  TS +W+   +K    G++ +YL    V TD KN++  +  + Q  LG+  + L  G ++  V +Y+NY  + AVLLG P+    K  L + + FEI LA+A+++  +  +   LYN + + +L    P + W++Y+N +            + Q+  N+ V+V +P Y + LT+ L    + +I +Y++WR  + S+ Y     + +  +F +  +GT+S + RWK C  A +  F   VG  YV  HF  EAK  + EMV  +   F
Sbjct:   85 DLCLSEGCIGTSHMILNNMDKTVDPCDDFYKFTCGGFEHRVVIQDDRSSSSQFSIIGDDLIQQLREILEAEPEEGESRVFTMARNAYKACMDEERIERIGLQPLQDMLKNLGGWPVLEENWDE--TSFSWIDSTYKFRRNGYSTDYLIDFSVVTDSKNSSWRLIDIDQASLGVSREYLINGLEDPDVISYFNYMVNTAVLLGAPRERAEKE-LKESLLFEIELAHASQAREERRNASRLYNPVLVKDLHNYAPMVPWLEYINNI--------LTEDIAQVDENERVIVDEPGYLKNLTILLPKFSNRSIANYMMWRTARASLGYFTESAQRIQLEFSKNITGTKSKTERWKACTGAATGSFSSPVGSMYVRKHFKEEAKQSMEEMVRDIRTEF 466          

BLAST of neprilysin-2-like isoform x3 vs. nr
Match: gi|1059405112|ref|XP_017775452.1| (PREDICTED: neprilysin-11 isoform X3 [Nicrophorus vespilloides])

HSP 1 Score: 321.242 bits (822), Expect = 4.669e-93
Identity = 148/300 (49.33%), Postives = 199/300 (66.33%), Query Frame = 0

Query:  636 DYKLLTGMLDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            ++K +   +DWMD  T   AL K++ +   I Y D +LNTT ++  Y  L+L P  +F++ L+ + +    S+N+L Q  ++  W    + A+VNAFYS + NSI  PAGILQG F+N +RP Y+NYG+IG++IGHE+THGFDD G+QFD+ GNL +WWE +T E F  KA C +DQY N+TVPE+ L + G + QGE IADNGGI  AY AY+ WVEKHG E  LPGL   S+QLFW SAAN WC ++ +E ++  V    H   KYRV   F N   F+KDF CP GS MNP+ KC++
Sbjct:  466 EFKEIVKQVDWMDNVTRKSALEKVDLMATHIAYPDELLNTTKLEEFYESLELEPNNYFKSILNMTLFGTHYSFNRLRQPVNKSEWITHGRPAVVNAFYSSIENSIQFPAGILQGVFFNNDRPKYMNYGAIGFVIGHEITHGFDDQGRQFDKNGNLVDWWEQETREAFVKKAQCIIDQYGNYTVPEIGLNLNGINTQGENIADNGGIKEAYLAYNKWVEKHGPEPKLPGLKYNSRQLFWISAANVWCAKTRSEELRQVVVTDEHSPDKYRVLGSFRNMDYFSKDFSCPEGSRMNPKTKCRV 765          

HSP 2 Score: 250.366 bits (638), Expect = 2.473e-67
Identity = 133/418 (31.82%), Postives = 228/418 (54.55%), Query Frame = 0

Query:   91 EICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPL--GPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSL-FQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFRIRLIQGKSYAAAAKKGFLVKV 505
            +IC TP C+  A ++++ MD ++DPC+DFY ++C  F K+  IPDD+SSV+  S+++D +  +LR + +E +  + ++  ++    +Q+CM+  ++EN GLQP+++     GGWP+  G      K     W Q  +    VG++ +Y     VG D KN+T  I  L Q  LGL  + L +G ++K+VQAYY Y  D+AV+ G  ++   +  L + ++FE+ LAN +       +  SLYN MT+G+L  KYP + W++Y+N           + SL F+I +++ V+V  P++       + +TP  T  +Y++WRA  +S  Y+  +++    ++    SG    +PRWK C+  ++    +  G  YV+ +F  +AK    EMVA +   F+  + Q        +K  L KV
Sbjct:   86 DICLTPGCIHTASKVLEAMDQSVDPCDDFYQFACGSFVKKTNIPDDKSSVTSFSVISDTLLEQLRSMIEEPIQPKEAKPFRLTKLIYQSCMNKTKIENEGLQPIKEIFSSIGGWPVLDGDNW---KDEDFDWKQAVYNFRKVGYSVDYFIDFSVGIDLKNSTKRIIDLDQSSLGLRREFLIKGMEDKLVQAYYEYMVDIAVIFGADRSR-AQQELKESLEFEMKLANISLPSEKRRNATSLYNPMTVGQLQIKYPSIPWLKYLN----------TLLSLDFEIGADEIVIVSVPQFISDFENLIATTPKRTQANYLMWRAAASSASYLTEELKNRQLKYSTILSGKTERAPRWKECIDVSAGSLSIAAGALYVKKYFNEDAKRNAIEMVADIRSEFKEIVKQVDWMDNVTRKSALEKV 489          

BLAST of neprilysin-2-like isoform x3 vs. nr
Match: gi|1059405107|ref|XP_017775450.1| (PREDICTED: neprilysin-11 isoform X1 [Nicrophorus vespilloides])

HSP 1 Score: 320.472 bits (820), Expect = 1.245e-92
Identity = 148/300 (49.33%), Postives = 199/300 (66.33%), Query Frame = 0

Query:  636 DYKLLTGMLDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            ++K +   +DWMD  T   AL K++ +   I Y D +LNTT ++  Y  L+L P  +F++ L+ + +    S+N+L Q  ++  W    + A+VNAFYS + NSI  PAGILQG F+N +RP Y+NYG+IG++IGHE+THGFDD G+QFD+ GNL +WWE +T E F  KA C +DQY N+TVPE+ L + G + QGE IADNGGI  AY AY+ WVEKHG E  LPGL   S+QLFW SAAN WC ++ +E ++  V    H   KYRV   F N   F+KDF CP GS MNP+ KC++
Sbjct:  481 EFKEIVKQVDWMDNVTRKSALEKVDLMATHIAYPDELLNTTKLEEFYESLELEPNNYFKSILNMTLFGTHYSFNRLRQPVNKSEWITHGRPAVVNAFYSSIENSIQFPAGILQGVFFNNDRPKYMNYGAIGFVIGHEITHGFDDQGRQFDKNGNLVDWWEQETREAFVKKAQCIIDQYGNYTVPEIGLNLNGINTQGENIADNGGIKEAYLAYNKWVEKHGPEPKLPGLKYNSRQLFWISAANVWCAKTRSEELRQVVVTDEHSPDKYRVLGSFRNMDYFSKDFSCPEGSRMNPKTKCRV 780          

HSP 2 Score: 250.751 bits (639), Expect = 2.631e-67
Identity = 132/416 (31.73%), Postives = 227/416 (54.57%), Query Frame = 0

Query:   91 EICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSL-FQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFRIRLIQGKSYAAAAKKGFLVKV 505
            +IC TP C+  A ++++ MD ++DPC+DFY ++C  F K+  IPDD+SSV+  S+++D +  +LR + +E +  + ++  ++    +Q+CM+  ++EN GLQP+++     GGWP+       K     W Q  +    VG++ +Y     VG D KN+T  I  L Q  LGL  + L +G ++K+VQAYY Y  D+AV+ G  ++   +  L + ++FE+ LAN +       +  SLYN MT+G+L  KYP + W++Y+N           + SL F+I +++ V+V  P++       + +TP  T  +Y++WRA  +S  Y+  +++    ++    SG    +PRWK C+  ++    +  G  YV+ +F  +AK    EMVA +   F+  + Q        +K  L KV
Sbjct:  101 DICLTPGCIHTASKVLEAMDQSVDPCDDFYQFACGSFVKKTNIPDDKSSVTSFSVISDTLLEQLRSMIEEPIQPKEAKPFRLTKLIYQSCMNKTKIENEGLQPIKEIFSSIGGWPV-LDGDNWKDEDFDWKQAVYNFRKVGYSVDYFIDFSVGIDLKNSTKRIIDLDQSSLGLRREFLIKGMEDKLVQAYYEYMVDIAVIFGADRSR-AQQELKESLEFEMKLANISLPSEKRRNATSLYNPMTVGQLQIKYPSIPWLKYLN----------TLLSLDFEIGADEIVIVSVPQFISDFENLIATTPKRTQANYLMWRAAASSASYLTEELKNRQLKYSTILSGKTERAPRWKECIDVSAGSLSIAAGALYVKKYFNEDAKRNAIEMVADIRSEFKEIVKQVDWMDNVTRKSALEKV 504          

BLAST of neprilysin-2-like isoform x3 vs. nr
Match: gi|1027910429|gb|AND99761.1| (neprilysin-2 [Monochamus alternatus])

HSP 1 Score: 317.005 bits (811), Expect = 1.377e-91
Identity = 145/292 (49.66%), Postives = 197/292 (67.47%), Query Frame = 0

Query:  644 LDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            +DWMD ET   AL K  S+   I Y D +L+   ++  Y  L+L P Q+ ++ L+ + +  + S+ +L Q  ++  W    + A+VNAFYS + NSI  PAGILQG F+N +RP Y+NYG+IG++IGHE+THGFDD G+QFD+ GNL +WW+ +T + F  KA C +DQY N++VPEL L + G + QGE IADNGGI  AY AY+ WVE++GEE  LPGL  T +Q+FW SAA+ WC+++  E ++  +    H   KYRV VP SN   FAKDFKCP+GS MNPE KC I
Sbjct:  467 VDWMDDETRQNALDKAKSMATHIAYPDELLDDNKLEEFYDGLELDPDQYMRSILNLTLFGTRFSFKRLRQPVNKTDWITHGRPAVVNAFYSSIENSIQFPAGILQGVFFNADRPRYMNYGAIGFVIGHEITHGFDDQGRQFDKNGNLVDWWQEETKKAFVEKAQCIIDQYGNYSVPELKLNLNGINTQGENIADNGGIKEAYLAYNRWVERNGEEPYLPGLRFTGRQMFWVSAASIWCSKTRNEELQQLIVTDEHAPDKYRVIVPLSNMEYFAKDFKCPMGSNMNPEKKCNI 758          

HSP 2 Score: 234.187 bits (596), Expect = 1.094e-61
Identity = 124/397 (31.23%), Postives = 209/397 (52.64%), Query Frame = 0

Query:   91 EICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPL--GPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLD-PEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFR 484
            ++C +P C+  A  +++ MD ++DPC+DFY ++C  F K+  IPDD+SSV+  S+++D +Q +LR + +E +  +  +  ++    ++ACM+   +EN GL  +   L+  GGWP+  GP           W +  +K   VG++ +Y     VG D KN+T  I  L Q  LGL  + L +G  +K+V AYYNY  D+AVL G  K   ++  L + ++FE+ LAN +       +  +LYN MT+ +L  K+P + W +Y+N +    ++V          +N EV+V+  P Y +     +  TP     +Y++WRA  +SV Y+  ++R     +    +G      RWK C+  ++    +  G  YV  +F  EA+    EMVA +   F+
Sbjct:   79 KVCVSPGCIHTASRVLEYMDPSVDPCDDFYEFTCGNFLKKTNIPDDKSSVTSFSIISDMLQEQLRTMIEEPVHEDEPKPFQLTKKLYRACMNKTLIENEGLDTIHSILEDLGGWPVLKGPMW---SEADFDWRKSVYKFRKVGYSVDYFIDFSVGVDVKNSTKRIIDLDQASLGLRREFLTKGLDDKIVSAYYNYMVDIAVLFGADKERAVRE-LRESLEFEMKLANISLPSEKRRNATALYNPMTIEQLQIKFPSIPWTEYMNTLLAPDTKV----------TNDEVIVVSVPSYIKNFEALVSRTPKRVQANYVMWRAAASSVSYLTEKLRKRQLDYTTIVTGRTEREARWKECIDISAGSLSIASGALYVRKYFNEEARQNAKEMVADIRAEFQ 461          

BLAST of neprilysin-2-like isoform x3 vs. nr
Match: gi|1005943670|ref|XP_015795185.1| (PREDICTED: membrane metallo-endopeptidase-like 1 isoform X3 [Tetranychus urticae])

HSP 1 Score: 316.62 bits (810), Expect = 2.442e-91
Identity = 141/301 (46.84%), Postives = 202/301 (67.11%), Query Frame = 0

Query:  635 KDYKLLTGMLDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            K++  +   +DWMD  T  RA  K N+I   IGY D +LN + +  LY +L L  + +F N  +  KW    ++N+L +   +  W +  +AA+VNA+Y+ L NSI  PAGILQG F++++RPNYLN+G+IG++IGHE+THGFDD G+QFD++GN KNWW++ T+++FR +  C +DQY N+T+PE  + + G + QGE IADNGG+  A+ AY  WV  HG+E  LPGL  ++ QLFW SAAN WC +   E ++  V  G H  +++RV  P SN  EF+K FKCP G+PMNP+ KC +
Sbjct:  469 KEFLTILDEVDWMDANTTQRAREKANAIVSYIGYPDELLNNSKVGELYTNLSLVERSYFTNVQNLRKWSTDFAFNKLRKPNLKGEWTKHARAAVVNAYYNALENSIEFPAGILQGSFFSKDRPNYLNFGAIGFVIGHEITHGFDDRGRQFDKDGNNKNWWDHVTDQKFRSRTQCIIDQYGNYTLPENGMKVNGINTQGENIADNGGLKEAFRAYQKWVFDHGKEPTLPGLSYSTDQLFWISAANVWCGKYRPEVLRLRVSLGSHSPSRFRVIGPMSNIEEFSKSFKCPRGTPMNPQKKCHV 769          

HSP 2 Score: 194.897 bits (494), Expect = 3.623e-48
Identity = 128/476 (26.89%), Postives = 228/476 (47.90%), Query Frame = 0

Query:   15 PP----KGKMDPALLKIGDSSILPCS--TMKLLGITLQANLGWTDNVAGLVTISALLAVFVVNTVLDGAIEDELGIRLRGGPEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGL-EYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAF 483
            PP    + +M+  L  I   S++ C   T+ L  +  Q N    + +    T   L+         D  I + L    +    +C T  CV AA ++I+N+D ++DPC+DFY ++C G+  R  IP+DR+SVS  S + D +  +LR + +        R   +  + +++CM+  E+E +G +P+ + L   GGWP+  + +    +S  W+    K    GF+ + L  L V TD++N T ++  L Q  LG+ +   L  G  +  VQ YY    + AV+LG  +    K  L + + FE  LAN +    +  ++  LYNLMT+ E+    P ++W ++ N +               I  N+ V+V  P Y ++L   L  T    + +Y++WR +      +  + R +A ++ +  +G   + PRW+ C+++ +    + +   YV++HF   +K    EMV  + + F
Sbjct:   16 PPWWNRRTRMERGLCTIAIVSLVMCLFLTIALAFVAYQYNANLVNGLGVSATADKLIGA------EDHHIYNSLE-ESQSQQTVCLTKGCVSAAADIIRNVDNSVDPCDDFYKFACGGWLDRHTIPEDRTSVSVFSAIQDDLNNKLRVLVESEAEPNEPRFYTMLRSMYKSCMNLTEIERVGNEPVLETLNELGGWPVLEQDKW-NASSFNWLDSLIKFRKAGFSHDILMDLSVNTDYRNNTRHVIDLDQATLGMPDRSYLLRGLNDSAVQGYYKLMVESAVMLGADRQKAEKDML-EALAFETILANFSIPREERRNISLLYNLMTVKEIETIAPNVNWSRFFNYLLNDP-----------IPPNELVLVNVPTYLKKLDKLLVETDGRIVANYMMWRIVLQCYTTLGKRWRELAQEYNKVITGQDREEPRWEQCMSSLTGSLGVALSSLYVKNHFKEGSKYMALEMVNYIHKEF 471          

BLAST of neprilysin-2-like isoform x3 vs. nr
Match: gi|1080048366|ref|XP_018565532.1| (neprilysin-2 isoform X1 [Anoplophora glabripennis])

HSP 1 Score: 316.62 bits (810), Expect = 2.847e-91
Identity = 145/300 (48.33%), Postives = 201/300 (67.00%), Query Frame = 0

Query:  636 DYKLLTGMLDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            +++ +   +DWMD ET   AL K  S+   I Y D +L+   ++  Y  L+L P+Q+ ++ L+ + +  + S+ +L Q  ++  W    + A+VNAFYS + NSI  PAGILQG F+N +RP Y+NYG+IG++IGHE+THGFDD G+QFD+ GNL +WW+  T + F  KA C +DQY N++VPEL L + G + QGE IADNGGI  AY AY+ WV+++GEE  LPGL  T +Q+FW SAA+ WC+E+  E ++  +    H   KYRV VP SN   FAKDFKCP+GS MNPE KC I
Sbjct:  470 EFQDILNNVDWMDDETRQNALDKAKSMATHIAYPDELLDDNKLEEFYDGLELDPEQYMRSILNLTLFGTRFSFKRLRQPVNKTDWITHGRPAVVNAFYSSIENSIQFPAGILQGVFFNADRPRYMNYGAIGFVIGHEITHGFDDQGRQFDKNGNLVDWWQEGTKKAFVEKAQCIIDQYGNYSVPELKLNLNGINTQGENIADNGGIKEAYLAYNRWVDRNGEEPYLPGLRYTGRQMFWVSAASIWCSETRNEELQQLIVTDEHAPDKYRVIVPLSNMEYFAKDFKCPVGSKMNPEKKCSI 769          

HSP 2 Score: 236.884 bits (603), Expect = 1.950e-62
Identity = 129/416 (31.01%), Postives = 216/416 (51.92%), Query Frame = 0

Query:   71 NTVLDGAIEDELGIRLRGGPEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPL--GPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFR 484
            NTV+  A +D    R      +C +P C+  A  +++ MD ++DPC+DFY ++C  F K+  IPDD+SSV+  S+++D +Q +LR + +E +  +  +  ++    ++ACM+   +EN GL+ +   L   GGWP+  GP     K     W +  +K   VG++ +Y     VG D KN+T  I  L Q  LGL  + L +G  +K+V+AYYNY  D+AVL G  +   ++  L + ++FE+ LAN +       +  +LYN MT+ EL  K+P + W +Y+N +    + V          +++ +VV  P Y +     +  TP     +Y++WRA  +SV Y+  ++R     +    +G      RWK C+  ++    +  G  YV  +F  EA+    EMVA +   F+
Sbjct:   76 NTVVINAPDDSTTER------VCVSPGCIHTASRVLEYMDPSVDPCDDFYEFTCGNFLKKTNIPDDKSSVTSFSIISDMLQEQLRTMIEEPIDPDEPKPFQLTKKLYRACMNKTLIENEGLETIHLILGELGGWPVLEGPMW---KEADFDWRKSVYKFRKVGYSVDYFIDFSVGVDVKNSTKRIIDLDQASLGLRREFLTKGLDDKIVRAYYNYMVDIAVLFGADRERAVRE-LRESLEFEMKLANISLPSEKRRNATALYNPMTIEELQSKFPSIPWTEYMNTLLAPDTRVT---------NDETIVVSVPSYIKNFEALVSRTPKRVQANYVMWRAAASSVSYLTDKLRKRQLDYTTIVTGRTEREARWKECIDISAGSLSIASGALYVRKYFNEEARQNAKEMVADIRAEFQ 472          

BLAST of neprilysin-2-like isoform x3 vs. nr
Match: gi|1005943668|ref|XP_015795184.1| (PREDICTED: membrane metallo-endopeptidase-like 1 isoform X2 [Tetranychus urticae])

HSP 1 Score: 316.62 bits (810), Expect = 3.699e-91
Identity = 141/301 (46.84%), Postives = 202/301 (67.11%), Query Frame = 0

Query:  635 KDYKLLTGMLDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            K++  +   +DWMD  T  RA  K N+I   IGY D +LN + +  LY +L L  + +F N  +  KW    ++N+L +   +  W +  +AA+VNA+Y+ L NSI  PAGILQG F++++RPNYLN+G+IG++IGHE+THGFDD G+QFD++GN KNWW++ T+++FR +  C +DQY N+T+PE  + + G + QGE IADNGG+  A+ AY  WV  HG+E  LPGL  ++ QLFW SAAN WC +   E ++  V  G H  +++RV  P SN  EF+K FKCP G+PMNP+ KC +
Sbjct:  480 KEFLTILDEVDWMDANTTQRAREKANAIVSYIGYPDELLNNSKVGELYTNLSLVERSYFTNVQNLRKWSTDFAFNKLRKPNLKGEWTKHARAAVVNAYYNALENSIEFPAGILQGSFFSKDRPNYLNFGAIGFVIGHEITHGFDDRGRQFDKDGNNKNWWDHVTDQKFRSRTQCIIDQYGNYTLPENGMKVNGINTQGENIADNGGLKEAFRAYQKWVFDHGKEPTLPGLSYSTDQLFWISAANVWCGKYRPEVLRLRVSLGSHSPSRFRVIGPMSNIEEFSKSFKCPRGTPMNPQKKCHV 780          

HSP 2 Score: 194.897 bits (494), Expect = 4.201e-48
Identity = 128/476 (26.89%), Postives = 228/476 (47.90%), Query Frame = 0

Query:   15 PP----KGKMDPALLKIGDSSILPCS--TMKLLGITLQANLGWTDNVAGLVTISALLAVFVVNTVLDGAIEDELGIRLRGGPEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGL-EYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAF 483
            PP    + +M+  L  I   S++ C   T+ L  +  Q N    + +    T   L+         D  I + L    +    +C T  CV AA ++I+N+D ++DPC+DFY ++C G+  R  IP+DR+SVS  S + D +  +LR + +        R   +  + +++CM+  E+E +G +P+ + L   GGWP+  + +    +S  W+    K    GF+ + L  L V TD++N T ++  L Q  LG+ +   L  G  +  VQ YY    + AV+LG  +    K  L + + FE  LAN +    +  ++  LYNLMT+ E+    P ++W ++ N +               I  N+ V+V  P Y ++L   L  T    + +Y++WR +      +  + R +A ++ +  +G   + PRW+ C+++ +    + +   YV++HF   +K    EMV  + + F
Sbjct:   27 PPWWNRRTRMERGLCTIAIVSLVMCLFLTIALAFVAYQYNANLVNGLGVSATADKLIGA------EDHHIYNSLE-ESQSQQTVCLTKGCVSAAADIIRNVDNSVDPCDDFYKFACGGWLDRHTIPEDRTSVSVFSAIQDDLNNKLRVLVESEAEPNEPRFYTMLRSMYKSCMNLTEIERVGNEPVLETLNELGGWPVLEQDKW-NASSFNWLDSLIKFRKAGFSHDILMDLSVNTDYRNNTRHVIDLDQATLGMPDRSYLLRGLNDSAVQGYYKLMVESAVMLGADRQKAEKDML-EALAFETILANFSIPREERRNISLLYNLMTVKEIETIAPNVNWSRFFNYLLNDP-----------IPPNELVLVNVPTYLKKLDKLLVETDGRIVANYMMWRIVLQCYTTLGKRWRELAQEYNKVITGQDREEPRWEQCMSSLTGSLGVALSSLYVKNHFKEGSKYMALEMVNYIHKEF 482          

BLAST of neprilysin-2-like isoform x3 vs. nr
Match: gi|1005943666|ref|XP_015795183.1| (PREDICTED: membrane metallo-endopeptidase-like 1 isoform X1 [Tetranychus urticae])

HSP 1 Score: 317.39 bits (812), Expect = 4.357e-91
Identity = 141/301 (46.84%), Postives = 202/301 (67.11%), Query Frame = 0

Query:  635 KDYKLLTGMLDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            K++  +   +DWMD  T  RA  K N+I   IGY D +LN + +  LY +L L  + +F N  +  KW    ++N+L +   +  W +  +AA+VNA+Y+ L NSI  PAGILQG F++++RPNYLN+G+IG++IGHE+THGFDD G+QFD++GN KNWW++ T+++FR +  C +DQY N+T+PE  + + G + QGE IADNGG+  A+ AY  WV  HG+E  LPGL  ++ QLFW SAAN WC +   E ++  V  G H  +++RV  P SN  EF+K FKCP G+PMNP+ KC +
Sbjct:  515 KEFLTILDEVDWMDANTTQRAREKANAIVSYIGYPDELLNNSKVGELYTNLSLVERSYFTNVQNLRKWSTDFAFNKLRKPNLKGEWTKHARAAVVNAYYNALENSIEFPAGILQGSFFSKDRPNYLNFGAIGFVIGHEITHGFDDRGRQFDKDGNNKNWWDHVTDQKFRSRTQCIIDQYGNYTLPENGMKVNGINTQGENIADNGGLKEAFRAYQKWVFDHGKEPTLPGLSYSTDQLFWISAANVWCGKYRPEVLRLRVSLGSHSPSRFRVIGPMSNIEEFSKSFKCPRGTPMNPQKKCHV 815          

HSP 2 Score: 195.282 bits (495), Expect = 5.209e-48
Identity = 128/476 (26.89%), Postives = 228/476 (47.90%), Query Frame = 0

Query:   15 PP----KGKMDPALLKIGDSSILPCS--TMKLLGITLQANLGWTDNVAGLVTISALLAVFVVNTVLDGAIEDELGIRLRGGPEICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGL-EYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAF 483
            PP    + +M+  L  I   S++ C   T+ L  +  Q N    + +    T   L+         D  I + L    +    +C T  CV AA ++I+N+D ++DPC+DFY ++C G+  R  IP+DR+SVS  S + D +  +LR + +        R   +  + +++CM+  E+E +G +P+ + L   GGWP+  + +    +S  W+    K    GF+ + L  L V TD++N T ++  L Q  LG+ +   L  G  +  VQ YY    + AV+LG  +    K  L + + FE  LAN +    +  ++  LYNLMT+ E+    P ++W ++ N +               I  N+ V+V  P Y ++L   L  T    + +Y++WR +      +  + R +A ++ +  +G   + PRW+ C+++ +    + +   YV++HF   +K    EMV  + + F
Sbjct:   62 PPWWNRRTRMERGLCTIAIVSLVMCLFLTIALAFVAYQYNANLVNGLGVSATADKLIGA------EDHHIYNSLE-ESQSQQTVCLTKGCVSAAADIIRNVDNSVDPCDDFYKFACGGWLDRHTIPEDRTSVSVFSAIQDDLNNKLRVLVESEAEPNEPRFYTMLRSMYKSCMNLTEIERVGNEPVLETLNELGGWPVLEQDKW-NASSFNWLDSLIKFRKAGFSHDILMDLSVNTDYRNNTRHVIDLDQATLGMPDRSYLLRGLNDSAVQGYYKLMVESAVMLGADRQKAEKDML-EALAFETILANFSIPREERRNISLLYNLMTVKEIETIAPNVNWSRFFNYLLNDP-----------IPPNELVLVNVPTYLKKLDKLLVETDGRIVANYMMWRIVLQCYTTLGKRWRELAQEYNKVITGQDREEPRWEQCMSSLTGSLGVALSSLYVKNHFKEGSKYMALEMVNYIHKEF 517          

BLAST of neprilysin-2-like isoform x3 vs. nr
Match: gi|1317162053|ref|XP_023244970.1| (neprilysin-2-like [Copidosoma floridanum])

HSP 1 Score: 314.309 bits (804), Expect = 1.088e-90
Identity = 140/301 (46.51%), Postives = 207/301 (68.77%), Query Frame = 0

Query:  635 KDYKLLTGMLDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEKHGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            K+++ +   +DWMD+ET + AL K++++N  I Y D +L+   +++ Y+ LD+ P  +F + L+ + +    S  QL    ++  W  +  AA+VNA+YSP  NS+  PAGILQG+F+N++RP Y+NYG+IG+++GHE+THGFDD G+Q+D+ GNL  WW+ +T ++F  KA C ++QY+N+T  E++L+I G + QGE IADNGGI  AY AY  WV ++G E+ LPGL+ T +Q+FW SAA  WCT+  +E +K ++    H   ++RV    SN  EFAKDFKCPLGS MNPE KC I
Sbjct:  445 KEFRKIIEKVDWMDEETRAAALDKIDAMNSFIAYPDELLDDKKLEHYYKALDISPGHYFDSVLNLTLFSYARSIEQLRLPVNKSDWVMYGDAAVVNAYYSPNDNSMQFPAGILQGEFFNKDRPQYMNYGAIGFVMGHEITHGFDDQGRQYDKNGNLIEWWKEETKKKFLQKAQCIINQYANYTASEVDLSINGVNTQGENIADNGGIKEAYLAYDEWVRRNGPEAQLPGLEYTPQQMFWISAATVWCTKQRSEALKLDIISDEHSPGEFRVLGSMSNMPEFAKDFKCPLGSRMNPEKKCSI 745          

HSP 2 Score: 221.476 bits (563), Expect = 2.590e-57
Identity = 118/381 (30.97%), Postives = 203/381 (53.28%), Query Frame = 0

Query:  105 LIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIP-LAFFQACMDSKEVENLGLQPLRDFLKIPGGWPLGPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAFR 484
            ++ NMD  +DPC++FY ++C GF K  +IPDD++SV   S++ D +Q E+R   +E +    SR  ++     + +C+    VE  GLQPL   +   GGWP+   +      +  W    ++   +GF+ ++    D+  D KN++  +  L Q  L L  + L +G +N++V AYY Y  D+A+LLG  +   ++  L + + FEI LAN +       +  +L N MT+ ELT  YP + W++Y+N++         +  + +I  N+ V V +P +   L   L+ TP   + +Y +WR ++ SV Y++  +R    +++ + +G     PRW+ C+   S+   L +G  YV  +F  EAK+ V EMVA + + FR
Sbjct:   80 ILGNMDPDVDPCDNFYEFACGGFMKNTIIPDDQTSVDTFSMIDDDLQMEVRASIEEGIKENESRPGRLRGKELYYSCLIETLVEEQGLQPLFKIIDRLGGWPV--LLEDYDDDAYDWKTSVYRNRRLGFSVDHFISFDIDIDQKNSSRRMISLDQAWLSLPREYLAKGPQNEVVSAYYRYMVDIAILLGANRDRAIEE-LRESLTFEITLANISLPSEKRRNATTLNNPMTVAELTAAYPSIPWLEYMNEL---------LDPVLEIDDNEVVNVDEPSFVSALEQLLRVTPKRVLANYAVWRVVRDSVDYLDDDIRKRQLEYWSEITGETEREPRWQECVGVVSSTMSLSIGALYVRKYFDEEAKNNVVEMVADIRKEFR 448          

BLAST of neprilysin-2-like isoform x3 vs. nr
Match: gi|1279738576|ref|XP_022914110.1| (neprilysin-2 isoform X3 [Onthophagus taurus])

HSP 1 Score: 314.694 bits (805), Expect = 1.329e-90
Identity = 142/293 (48.46%), Postives = 205/293 (69.97%), Query Frame = 0

Query:  644 LDWMDKETASRALIKLNSINELIGYDDVILNTTAMDYLYRDLDLHPKQFFQNALSRSKWWLKDSWNQLNQSTSEDPWPEFDQAAIVNAFYSPLSNSINLPAGILQGKFYNENRPNYLNYGSIGYIIGHELTHGFDDSGKQFDEEGNLKNWWENKTNEEFRFKADCFVDQYSNFTVPELNLTIEGKHIQGEGIADNGGIMHAYDAYSAWVEK-HGEESILPGLDLTSKQLFWASAANTWCTESNTENIKYNVKYGVHPLAKYRVNVPFSNSREFAKDFKCPLGSPMNPELKCKI 935
            +DWMD+++  RAL K  +++  I Y D +L+   ++  Y+DL+L  +++  + ++ +++  + S+N+L Q  ++  W    + AIVNAFYS + NSI  PAGILQG F++ +RP Y+NYG+IG++IGHE+THGFDD G+QFD++GNL +WWE +T + F  KA C +DQYSNFTVPEL + ++G + QGE IADNGGI  AY AY AW+++ +  E  LPGL+ T KQ+FW SAA TWC++   E +K  V   +H   + RV  P  N +EF++DF CPLGSPMNPELKC +
Sbjct:  473 VDWMDEKSRKRALEKAQAMSTHIAYPDELLDDKILEKFYQDLELDSEKYLMSVINLTQFATRFSFNRLRQPVNKTDWITHGRPAIVNAFYSAIENSIQFPAGILQGVFFSNDRPKYMNYGAIGFVIGHEITHGFDDQGRQFDKDGNLVDWWEPETQQSFVKKAQCIIDQYSNFTVPELGINLKGVNTQGENIADNGGIKEAYYAYEAWMKRTNTTEPQLPGLNYTPKQMFWLSAAATWCSKYRKEALKVRVLTSLHSPGRQRVIGPLRNLKEFSRDFNCPLGSPMNPELKCTV 765          

HSP 2 Score: 232.261 bits (591), Expect = 6.014e-61
Identity = 124/395 (31.39%), Postives = 212/395 (53.67%), Query Frame = 0

Query:   91 EICQTPNCVMAAQELIQNMDLTLDPCEDFYAYSCSGFQKRLMIPDDRSSVSETSLLADQVQYELRQIFDETLPLESSRGAKIPLAFFQACMDSKEVENLGLQPLRDFLKIPGGWPL--GPKMRTKKGTSTTWVQLAHKLMDVGFTGNYLFGLDVGTDFKNTTNNIFFLAQPRLGLEYKELKEGWKNKMVQAYYNYTYDMAVLLGEPKTTDLKSTLTKMIQFEINLANATRSWADVSDVDSLYNLMTLGELTQKYPFLSWVQYVNQVGRSKSEVAQMYSLFQIQSNQEVVVLDPEYFERLTMTLKSTPDTTIQSYIIWRAIKTSVPYMNSQVRAVASQFYRQFSGTRSDSPRWKGCLTATSNFFPLLVGKFYVESHFPPEAKDKVSEMVALVTEAF 483
            ++C TP C+  A ++++ +D ++DPC+DFY ++C  F K+  IPDD+SSV+  S+++D +Q +LR + +E +  +  +   +    ++ACM+   +E  GL+ ++  +   GGWP+  G K    K     W Q  +K    G++ +YL    V  D KN+T  +  L Q  LGL  + L +G ++K+V+AYY+Y  D+AV+ G  KT   K  L + + FEI LAN +       +  +LYN MT+ EL  KYP ++W +Y N +    ++V           N+ V+V  P+Y +     +K+T   T  +Y++WRA+ +SV Y+N ++R    Q++   +G    + RWK C+   +       G  YV  +F  +A+    +MV  +   F
Sbjct:   85 KVCLTPGCIHTASKVLETIDESVDPCDDFYDFACGNFVKKTNIPDDKSSVTTFSVISDLLQEQLRTMIEEPIRKDEPKPFVLTKKLYRACMNKTLIEKDGLKTIKGIVNELGGWPVLEGDKF---KDQEFDWRQTIYKFRKSGYSVDYLIDFSVAIDLKNSTKRVIDLDQASLGLRREFLIKGVEDKLVKAYYDYMVDIAVIFGANKTNAEKE-LKESLDFEIKLANISLPNEKRRNASALYNPMTVSELQSKYPIVNWKEYFNVLLAPDTKV---------DDNEVVIVSIPKYLKDFDDLIKNTSKRTQANYLLWRAVASSVSYLNDELRNRQLQYHTIATGKTERASRWKECIEIAAGSLGNAAGALYVRKYFNEDARQNAIKMVKDIRTEF 466