BLAST of two pore channel 1 vs. L. salmonis genes
Match: EMLSAG00000002076 (supercontig:LSalAtl2s:LSalAtl2s1389:19391:25929:1 gene:EMLSAG00000002076 transcript:EMLSAT00000002076 description:"maker-LSalAtl2s1389-snap-gene-0.16")

HSP 1 Score: 841.262 bits (2172), Expect = 0.000e+0
Identity = 438/788 (55.58%), Postives = 554/788 (70.30%), Query Frame = 0

Query:   99 QWEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGVVDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTY-------------RG--------ITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSN-YNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAEIQDLAIPPNSSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQ---------SRGRLEAAILAESAA---NNYHAHGDNEHIQIVQDEGSITTIHRHVLPD 852
             W+ NY+EA+IFLEEGLNNEKF  HP   EALPAYL+VHN  F+  DL A++VL+GL FFE PC   +  P  IH+ IEL +LVL+ +Q +LKTRWIGWKDFF HKRT  K+ TL VM++E + ++VR+  HFRV+RALRPIF+IDNHYC GVRRFIRQILQSLPPI DM+ L+ FVM+IYSVFGFYLF   D  NF      F+SLFVLLTTANYPDVMM+SY++S WSS FF+SYLSINLY LMNLMLAVVYD FT+IEK+KFR+L+LHKRKAAQHAF LLVT+ER   +SF HF GL+  Y             RG          + TEA+LIF+ LNKS   ++ +DEFY +++AC Y    ++ +E W+++  +    K+   I ++VTS WF++T   +VL NGIIL+VQ   M   S+     IY  W+SYFF+                       WN FDF  TVLGILSLI E L  P+F++VILRPLRLL+LFR+K+RFRDVFGT VILYPR+ SA+IV++LIYYFF IIGMECF  + LK CCKN+SVEQ++ + EGS++N+YFYLNNF  L  S +TLFELTVVNNW+VIMEG+A V     SR+FFM FY+FTMIVMTIIVAFILEAFLFRIQYK+  TKEDE K L  EV+++S+E+    I +G       + PN+ F+F G KSR K QLQ+LMY++EMD W+A+AQ +ES          +RGR E++     A     N ++ G + HI +  +EG ++TI+R ++ D
Sbjct:   52 HWDSNYREASIFLEEGLNNEKFNHHPCSSEALPAYLLVHNVVFNVIDLCASIVLLGLAFFEDPCLPSVKFPPIIHAGIELCSLVLVAVQVLLKTRWIGWKDFFXHKRTAFKIGTLFVMVIETLTVMVRNQTHFRVTRALRPIFIIDNHYCWGVRRFIRQILQSLPPIFDMMGLIVFVMIIYSVFGFYLFSDWDPDNFQTFVQSFVSLFVLLTTANYPDVMMKSYHKSFWSSTFFISYLSINLYILMNLMLAVVYDTFTKIEKDKFRKLYLHKRKAAQHAFKLLVTRERKNVISFNHFRGLITFYKPSACKKEKDFFFRGQDLICAFLFLADTEAFLIFKFLNKSQLKFITLDEFYNVYDACVYKXRPAKSDEPWYNDSISGCTKKIFELINKIVTSAWFDHTF--VVLINGIILLVQAGVM--ASAVEDQYIYVTWISYFFIG----------------------WNTFDFITTVLGILSLIFEYLGTPIFFIVILRPLRLLSLFRIKQRFRDVFGTTVILYPRMVSAVIVIILIYYFFAIIGMECFHGLPLKNCCKNTSVEQFYKFEEGSSSNLYFYLNNFNDLASSGVTLFELTVVNNWFVIMEGFAHVKNES-SRLFFMVFYLFTMIVMTIIVAFILEAFLFRIQYKECLTKEDEIKKLMTEVVISSDEVNDY-IRKGQSSNQTPVLPNAVFRFNGYKSRTKTQLQQLMYANEMDGWIAEAQSEESNIIARALNSSNRGRCESSEDNGDAIFFHQNQNSGGASVHI-VDDEEGEVSTINRRIISD 810          

BLAST of two pore channel 1 vs. L. salmonis genes
Match: EMLSAG00000006164 (supercontig:LSalAtl2s:LSalAtl2s336:580767:583352:-1 gene:EMLSAG00000006164 transcript:EMLSAT00000006164 description:"maker-LSalAtl2s336-snap-gene-6.53")

HSP 1 Score: 188.734 bits (478), Expect = 6.367e-51
Identity = 175/652 (26.84%), Postives = 316/652 (48.47%), Query Frame = 0

Query:  195 IGWKDFFKHKRTLIKVSTLVVM-LLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGVVDQVN------FGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVT----KERP-----------KEVSFQHFEGLVRTYRGITSPTEAY----LIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNY--NVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLG-ILSLILEVLH------IPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEG---SNANVY---FYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTF-YIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGA-----------EIQDLAIPPNSSFKFTGEKSRNKEQLQRLMYSDEM 793
            I WKD  KH    I V+ L+++ +L+ ++    +    R SR LRP+F ++      +R+  R I ++LP I  +LVL+   + I+++ G+ LF   + V       F +       ++VL+T+AN PDVMM +Y+ SRW ++FF++Y+ + LY  MN++LAV+Y+ + R  + +    +  K +     F LLV+    +E P            +  F + +  +R  + IT P E+      ++ +L+ +    +   EF ++ +     ++   +   +       +Y  K+  FIK +V + WF Y   +I++ N   +      +D           A W   +F+ L+TLE +LK+   G K +    WN+FD  V     I+S+  E L+      I L  ++ LR LRL  +F   +RFR V  T + + P L++   +L++ +YF+ +IGME F D  +     NSS E +          +++ Y   +  NNF ++  S + LFEL VVN W+V+ EGY +V +   +R++F++F  I   +++ I  AF+LEAF+           + +++ L +   L S++I  + +A G            +  D ++  +  F  +    R+  +L   M+  E+
Sbjct:   11 IFWKDP-KH----ISVAGLILLNVLDVLIYAGLNGGGIRFSRPLRPLFFVNFPEGRQIRQAFRNIRRTLPEIFRVLVLLMLSIAIFTLMGYKLFCQKNLVKANGSPYFMNYFDTLWDMYVLVTSANNPDVMMPAYDYSRWYAVFFLTYIVLTLYIFMNVVLAVIYNNYKRNLRNEITDFYQRKCELLIEVF-LLVSVPDVQETPGPKNQNDGIFDAKNRFLNHDDFIRLLQ-ITFPGESLKYYEALWLILDTNGDRRIDFKEFMQLDDLIHIPYTDLNECLTFLERKLPGLYTSKISDFIKNIVRNMWFRYVFDLIIIVNAFFIAFD---LDG----------AEW---YFLVLFTLEIILKVYTFGPKLFVKKLWNLFDLLVVGSALIVSVAQESLNEVQESVISLDVLLALRVLRLFKIFHSIQRFRTVLNTILYIVPSLSTYGGILMIFFYFYALIGMEIFGD-RVHSVGYNSSAEDFDPNCGNPLLDSSDFYRNHYCANNFNNVVSSFVLLFELMVVNQWHVLTEGYVLVMQSKWTRLYFLSFNLICVTVILNIYTAFVLEAFIMEYAALHISPSQSQEQDLPLAFSL-SKKINEMGLAFGKKGVDDSGMVSEDFDDYSLKTSIRFSLSTRPPRSGYKLLEKMFCQEL 637          

BLAST of two pore channel 1 vs. L. salmonis genes
Match: EMLSAG00000010843 (supercontig:LSalAtl2s:LSalAtl2s721:13130:108134:1 gene:EMLSAG00000010843 transcript:EMLSAT00000010843 description:"maker-LSalAtl2s721-augustus-gene-1.27")

HSP 1 Score: 78.5666 bits (192), Expect = 5.353e-15
Identity = 68/273 (24.91%), Postives = 120/273 (43.96%), Query Frame = 0

Query:  470 VTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLH-----IPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIM-----------------EGYAIVTEVGLSRVFFMTFYIF-TMIVMTIIVAFILEAF 719
            VTS  FEYTI  ++L N + L ++  F   P + +A   +   ++  F   +TLE V KL    FKNYF   WN FDF + +  ++ + +  L+     I + +  + R +RL+ L    +  R +  T    +  L    I++ LI++ +G++GM+ F  +  +               EGSN N     NNFQ+   + + LF       W  IM                  G         +  +F++F++  + +++ + VA I++ F
Sbjct: 1352 VTSPVFEYTIFAMILINTLSLAMK--FYQQPQTYTA---FLDVLNVIFTVFFTLEFVFKLGAFRFKNYFGDPWNSFDFIIVLGSLIDIAMAELNPGNNMISINFFRLFRVMRLVKLLSKGEGIRTLLWTFFKSFQALPWVAILIGLIFFIYGVVGMQVFGKIAPQ---------------EGSNINRN---NNFQTFLQALLVLFRSATGEAWQEIMLSCRKHPDVICDERSEDRGNPGGCGTDFAYPYFISFFVVCSFLILNLFVAVIMDNF 1601          

HSP 2 Score: 72.0182 bits (175), Expect = 5.317e-13
Identity = 71/274 (25.91%), Postives = 112/274 (40.88%), Query Frame = 0

Query:  475 FEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGF----KNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYV-----EGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLR 739
            FE+ I + +L N + L + T F      T+  N     V Y F+ ++T E V+K++  GF      Y  S WN+ DF + ++G++S IL  L I  F V  LR  R+L   R+           V   PR                    E  +  N   C  N   ++   YV     EG N    F + NF    +S +T+F+   +  W  ++  Y +   +G S  F    Y  + +V+    AF +   +  +   + F+KE EK   R
Sbjct:  174 FEFLILLTILGNCVALAIYTPF--PAEDTNETNQILEKVEYIFLVIFTTECVMKIIAYGFWQHPTAYLRSAWNMLDFTIVMIGVISTILSSLRIEGFDVKALRAFRVLRPLRL-----------VSGVPR--------------------EMEETDNPTLCGGNYKCDEEAGYVCREYWEGPN----FGITNFDHFGLSMLTVFQCITLEGWTDVL--YWVQDAMGSSWQF---IYFVSXVVLG---AFFVMNLILGVLSGE-FSKEREKAQSR 401          

HSP 3 Score: 64.6994 bits (156), Expect = 1.109e-10
Identity = 51/191 (26.70%), Postives = 92/191 (48.17%), Query Frame = 0

Query:  441 SWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVV----QTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHI-PLFYVVILRPLRLLTLFRMKK---RFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECF 623
            SW Q +++       N    ++C   +++V S    + I ++V  N  +L      Q  ++D          +  + + FFV L+T E  LK+  +GF+ YF S +N FD  V +  IL LIL   +I P   + +LR +RLL  F++ +      ++  + V     + S +++L L  + F ++GM+ F
Sbjct:  574 SWFQKKKKA--LEKLNRRARRVC---RKIVKSXAMFWIIIILVFLNTCVLATEHYKQPIWLDD---------FQEFTNLFFVVLFTFEMFLKMYSLGFQGYFVSLFNRFDCFVVISSILELILTNENIMPPLGLSVLRCVRLLRTFKVTRYWSSMGNLVKSLVNSIASINSLLVILFLFIFIFSLLGMQIF 750          

HSP 4 Score: 58.5362 bits (140), Expect = 8.702e-9
Identity = 41/168 (24.40%), Postives = 79/168 (47.02%), Query Frame = 0

Query:  462 LCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGF----KNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVV-ILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQ 624
            LC FI      ++F   + V ++ +  +L  +    D  +S S  N    +  YFF  ++T+E  LK++  GF      +  S +N+ D  V  + ++S I     I +  ++ +LR LR L      K  + V    ++    + + ++V LL+ + F +IG++ F+
Sbjct: 1028 LCHFI---CNHSYFGNIVLVCIMVSSAMLAAE----DPLNSNSERNKILNYFDYFFTTIFTIEVCLKVISYGFLLHPGAFCRSGFNLLDLLVVAVSLISFIFSSGAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAXKTIGNIVLVTLLLQFMFAVIGVQLFK 1188          

BLAST of two pore channel 1 vs. L. salmonis genes
Match: EMLSAG00000010768 (supercontig:LSalAtl2s:LSalAtl2s712:648445:661651:1 gene:EMLSAG00000010768 transcript:EMLSAT00000010768 description:"maker-LSalAtl2s712-snap-gene-6.14")

HSP 1 Score: 65.0846 bits (157), Expect = 8.065e-11
Identity = 38/130 (29.23%), Postives = 70/130 (53.85%), Query Frame = 0

Query:  504 SANNIYAPWVS-------YFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFR---DVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECF 623
             AN   +P++S       YFF  ++ +E+ +KLV +  + +FS  WN FDF + VL ++ L+ E +      + +LR  RLL +F++ K ++   D+       +  L++   VL +I + F ++GM+ F
Sbjct:  775 DANGGMSPFLSSVLTQGNYFFTTIFAVESFIKLVAMSPRYFFSEGWNCFDFLIVVLSLVELLAEGVS----GLSMLRSFRLLRVFKLAKSWKSLNDILTIMANTFGALSNLTFVLCIIIFIFAVMGMQLF 900          

HSP 2 Score: 61.2326 bits (147), Expect = 1.026e-9
Identity = 55/238 (23.11%), Postives = 110/238 (46.22%), Query Frame = 0

Query:  458 YIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDF---AVTVLGILSLILEVLHIPLFYVV-ILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYF------YLN---NFQSLHISAITLFELTVVNNWYVIME 682
            Y H+L   + R++ + +FE  +  ++L +  ++ ++  + D+      + +Y  ++      ++ LE +LKL  +G   YF + W   DF   AV+++   + ++ + +IP+F  +  LR LR L      +  + V    V   P + + ++V L+ +  F IIG+  F     K  C +    + FS+    N  V        + N   NF ++ I+ + LF++     W  IM+
Sbjct: 1172 YRHRLQ--VSRLIENKYFEGVVLTLILLSSFVMTLEDIWFDT-RPLLVDMLY--YLDRILTVVFFLETLLKLFAMGCVMYFGNAWCWLDFVIVAVSLINFGASLVGLGNIPIFKTMRTLRALRPLRAMAKMEGMKVVVNALVGALPSIFNVLLVCLIFWLIFAIIGINTFMGKFYK--CLDEETGEKFSHEIIPNKTVCLNETGAVWTNARVNFDNVFIAYLALFQVATFKGWMTIMD 1402          

HSP 3 Score: 57.3806 bits (137), Expect = 1.696e-8
Identity = 34/120 (28.33%), Postives = 63/120 (52.50%), Query Frame = 0

Query:  517 FVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLF-------YVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLK 629
            F+ ++T E +LK+  +   +YF+  WN+FDF V +L +  L L  L    F        V + +  R+L L +  K  R +  + V+ +P L +  ++L L+ + F + GM  F++V ++
Sbjct: 1520 FIVIFTAEMLLKMFALR-HHYFAEPWNLFDFVVVMLSLAGLFLSDLIEKYFVSPTLLRVVRVAKVGRVLRLIKGAKGIRTLLFSLVMAFPALVNICLLLFLVMFIFAVFGMSLFKNVKIR 1638          

BLAST of two pore channel 1 vs. L. salmonis genes
Match: EMLSAG00000004952 (supercontig:LSalAtl2s:LSalAtl2s260:1045447:1062229:1 gene:EMLSAG00000004952 transcript:EMLSAT00000004952 description:"maker-LSalAtl2s260-snap-gene-10.24")

HSP 1 Score: 62.3882 bits (150), Expect = 4.365e-10
Identity = 58/233 (24.89%), Postives = 105/233 (45.06%), Query Frame = 0

Query:  466 IKRMVTSNW--FEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGF----KNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRD--VFGTAVIL--YPRLTSAIIVLLLIYYFFGIIGMECFQ----------------DVNLKECCKNSSVEQYF--SYVEGSNANVYFYLNNFQSLHISAITLFE 670
            +K +    W  FE+ I   +LA  + L +   + +    T+  N++   +   F+ ++T E V+K+V  G     + Y  + WN  DF++ ++G + ++L  L I  F V  LR  R+L   R+        V   A+++  +P    A++VL +I   + IIG++ FQ                D + + C  N   E      Y+ G N    F + NF +  +S +T+F+
Sbjct:   59 LKCITVVEWKAFEFFILATILATCVSLALHKPYPNG--DTNDLNLFLEKIEVIFMVIFTGECVIKIVAYGLYQHEEAYLRNTWNFIDFSIVMIGFMDILLSSLSIEGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILMAIFPLFHIALLVLFVI-IIYSIIGLQMFQGLMHKACFDNITGVLIDDDPRLCGGNYQCETGVCREYIPGPN----FGITNFDNFILSMLTVFQ 284          

HSP 2 Score: 62.3882 bits (150), Expect = 5.042e-10
Identity = 62/272 (22.79%), Postives = 116/272 (42.65%), Query Frame = 0

Query:  472 SNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIP------LFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIM-------------EGYAIVTEVGLSRV----FFMTFYIFT-MIVMTIIVAFILEAF 719
            S+ FEY +   +L N I L +  +F + P   S    +   ++Y F   + LE V+K+     K YF   WN FD  + +   + + +  L         + +  + R LRL+ L    +  R +  T +  +  L   ++++ L+++ +G++GM+ F  + + E   ++S+         SN       NNFQ+   + I LF       W  IM             E        G   +    +F++F+I T  +++ + VA I++ F
Sbjct: 1002 SSKFEYVVFGAILLNTISLAM--SFYNQPQIYSD---FLDLLNYIFTIFFALEFVIKMAAFRPKEYFCDPWNTFDCIIVIGSFIDIGMSSLGSGDGGGISINFFRLFRALRLVKLLSKDESIRTLLWTFIKSFQALPYVMLLIALVFFIYGVVGMQVFGKIAINE---DTSIH--------SN-------NNFQNFQSALIVLFRSATGEAWQDIMLSCIPSPDVECDEESEEAGNPAGCGSIIAYPYFISFFIATSFLILNLFVAVIMDNF 1250          

BLAST of two pore channel 1 vs. L. salmonis genes
Match: EMLSAG00000009714 (supercontig:LSalAtl2s:LSalAtl2s627:140867:158129:1 gene:EMLSAG00000009714 transcript:EMLSAT00000009714 description:"maker-LSalAtl2s627-snap-gene-1.23")

HSP 1 Score: 62.3882 bits (150), Expect = 5.136e-10
Identity = 33/128 (25.78%), Postives = 69/128 (53.91%), Query Frame = 0

Query:  514 SYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFR---DVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVE 638
            +YFF  ++  E+ +KL+ +  + +F+  WN FDF + +L     ++E+L   +  + +LR  RLL +F++ K ++   D+          L++  +VL +I + F ++GM+ F    +++ C+    E
Sbjct:  668 NYFFTTIFAFESFMKLMAMSPRYFFADGWNCFDFLIVILS----LVELLAXGVNGLSMLRSFRLLRVFKLAKSWKSLNDIMTIMANTLGALSNLTVVLCIIIFIFAVMGMQLFGSFYIEKACEKWKCE 791          

BLAST of two pore channel 1 vs. L. salmonis genes
Match: EMLSAG00000007396 (supercontig:LSalAtl2s:LSalAtl2s420:189079:382322:1 gene:EMLSAG00000007396 transcript:EMLSAT00000007396 description:"maker-LSalAtl2s420-snap-gene-3.14")

HSP 1 Score: 58.5362 bits (140), Expect = 5.883e-9
Identity = 36/141 (25.53%), Postives = 74/141 (52.48%), Query Frame = 0

Query:  488 IILVVQTAFMDSPSSTSANNIYAPW--VSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRF---RDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECF 623
            I ++  TAF+ +     +N++       +  F + +TLEA+LKL+ +  K YF S WNIFD  +    +L L +E ++     + +LR +RL+ + ++ + +   + +    +     L +   +L+++ Y F ++GM+ F
Sbjct:  476 ICIIANTAFLAAEHHGMSNDLKHVLNIGNKVFTSFFTLEAILKLIALS-KEYFESGWNIFDLIIVFASLLDLSVESVN----GISVLRGMRLMRVLKLAQSWTTMKVLLSIIISTLGALGNLTFLLIIVIYIFAVLGMQLF 611          

BLAST of two pore channel 1 vs. L. salmonis genes
Match: EMLSAG00000006480 (supercontig:LSalAtl2s:LSalAtl2s354:213665:368876:-1 gene:EMLSAG00000006480 transcript:EMLSAT00000006480 description:"maker-LSalAtl2s354-snap-gene-2.25")

HSP 1 Score: 58.5362 bits (140), Expect = 6.750e-9
Identity = 45/169 (26.63%), Postives = 80/169 (47.34%), Query Frame = 0

Query:  461 KLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFR------DVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECF 623
            KL  ++  ++   + +  I + +L N   + +    MD     +  N      +YFF A + +E+  KL+ +  K YF   WNIFDF +  L ++ L L    + +  + +LR  RLL +F++ K +        + G  V     LT   +VL +I + F ++GM+ F
Sbjct:  700 KLTEYLSFIIFDPFVDLFITLCILVNTFFMGLDHHDMDPDIHKTLQN-----GNYFFTATFAIESCXKLMAMSPKYYFLEGWNIFDFIIVSLSLIELGL----VNVSGLTVLRTFRLLRVFKLAKSWPTLNLLISIMGKTVGALGNLT---LVLCIIIFIFAVMGMQLF 856          

BLAST of two pore channel 1 vs. L. salmonis genes
Match: EMLSAG00000008334 (supercontig:LSalAtl2s:LSalAtl2s508:27226:174463:1 gene:EMLSAG00000008334 transcript:EMLSAT00000008334 description:"maker-LSalAtl2s508-snap-gene-1.26")

HSP 1 Score: 55.8398 bits (133), Expect = 4.260e-8
Identity = 42/156 (26.92%), Postives = 68/156 (43.59%), Query Frame = 0

Query:  477 YTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGF----KNYFSSYWNIFDFAVTVLGILSLILEVLHIP----LFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQ 624
            + +CVIVL++     +  A  D     S  N    +  Y F  ++TLE  LK++ +GF      Y   +WN  D  V    ++S    +   P    L  + +LR LR L       + + VF   VI    + + +IV +L    F I+G++ F 
Sbjct:  687 FIMCVIVLSS-----IALASEDPVDENSIRNKLLGYADYGFTTIFTLECSLKMIDLGFVLHPGAYLRDFWNFLDITVVSCALISFYHTLAGTPTGQKLKSLKLLRVLRPLKTINRVPKLKAVFDCVVISLKNVFNILIVYMLFQIIFAIVGVQLFN 837          

BLAST of two pore channel 1 vs. L. salmonis genes
Match: EMLSAG00000007884 (supercontig:LSalAtl2s:LSalAtl2s464:432083:573266:-1 gene:EMLSAG00000007884 transcript:EMLSAT00000007884 description:"maker-LSalAtl2s464-augustus-gene-6.21")

HSP 1 Score: 55.8398 bits (133), Expect = 4.495e-8
Identity = 70/315 (22.22%), Postives = 133/315 (42.22%), Query Frame = 0

Query:  455 YNVYI----HKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMD--SPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGF----KNYFSSYWNIFD-------FAVTVLGIL----SLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVY--------------FYLN---NFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSR-----------VFFMTFYIFT-MIVMTIIVAFILEAF 719
            Y +Y+    HK+      + +  WF++ + + + +N I L ++   +   SP     N + +      F  ++T+E  +K +  G     + YF + WNI D       F  TV G+L    S I ++L +    V  LRPLR++         + V  T +     + + +++    +  FGI+G++ F+ +              F Y EG +AN+                ++N   NF +L  + ++LF L+  + W  IM  Y  +  VGL +           ++F++F +     V+ + V  ++E F
Sbjct: 1268 YTLYVFHPNHKIRLKCTEITSRGWFDFIVLLFIASNCITLAMERPNIPPWSPERKLLNVLNS-----IFTFIFTIEMAMKAISTGLIYGAEAYFKNGWNIMDGSLVIVSFVDTVSGLLLGKSSKIFDILRV-FRLVRALRPLRVIN---RAPGLKLVVQTLLSSLKPIGNIVLICCTFFIIFGILGVQLFKGI--------------FYYCEGPHANIVKNKTQCLELSDKGNRWINHKYNFDNLGHALMSLFVLSSKDGWVNIM--YQGLDAVGLDQQPKENFNEWRLIYFISFLLLVGFFVLNMFVGVVVENF 1557          

HSP 2 Score: 55.8398 bits (133), Expect = 5.018e-8
Identity = 61/296 (20.61%), Postives = 123/296 (41.55%), Query Frame = 0

Query:  120 FTFHPRCHEALPAYLMVHNNWFHFFDL---GAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVL-ITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNH--------FRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGVV------------------------------DQVNFGDLQTCFISLFVLLTTANYPDVMMRSY-----------NQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEK 362
            + FHP     L    +    WF F  L    +  + + +     P   P     ++ +SI      + + ++AI      G + +FK+   ++  S ++V  ++ V  L+   +         FR+ RALRP+ +I+     G++  ++ +L SL PI +++++     +I+ + G  LF  +                               + NF +L    +SLFVL +   + ++M +             N + W  I+F+S+L +  +F++N+ + VV + F R   E+
Sbjct: 1271 YVFHPNHKIRLKCTEITSRGWFDFIVLLFIASNCITLAMERPNIPPWSPERKLLNVLNSIFTFIFTIEMAMKAISTGLIYGAEAYFKNGWNIMDGSLVIVSFVDTVSGLLLGKSSKIFDILRVFRLVRALRPLRVINR--APGLKLVVQTLLSSLKPIGNIVLICCTFFIIFGILGVQLFKGIFYYCEGPHANIVKNKTQCLELSDKGNRWINHKYNFDNLGHALMSLFVLSSKDGWVNIMYQGLDAVGLDQQPKENFNEWRLIYFISFLLLVGFFVLNMFVGVVVENFHRCRAEQ 1564          

HSP 3 Score: 54.299 bits (129), Expect = 1.629e-7
Identity = 38/134 (28.36%), Postives = 69/134 (51.49%), Query Frame = 0

Query:  452 FSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWV-SYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYV--VILRPLRLLTLFR 582
            +S+Y + +HK+       VTS +F   I  ++  N I + ++   M  P   +    YA  V +YFF A++ LEA +K+  +G   Y S  WN  D  + +L ++ ++LE +   +  +   I+R +R+L + R
Sbjct: 1596 YSHYRLSVHKV-------VTSKYFXLAISAVIGLNVITMAMEFYMM--PVVLA----YALKVCNYFFTAVFILEAGMKISALGIGRYLSDKWNQLDVVIVLLSVVGIVLEEIKSDMMPINPTIMRVMRVLRIAR 1716          

BLAST of two pore channel 1 vs. SwissProt
Match: gi|123797698|sp|Q9WTN5.2|TPC1_RAT (RecName: Full=Two pore calcium channel protein 1; AltName: Full=Voltage-dependent calcium channel protein TPC1)

HSP 1 Score: 582.408 bits (1500), Expect = 0.000e+0
Identity = 330/776 (42.53%), Postives = 478/776 (61.60%), Query Frame = 0

Query:   70 IEQEMIPSTD---------QRPSLSVEDMDAHPQWM-REQWEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGV-VDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHI-PLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANV------YFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDE-------KKTLRVEVLLTSEEIRRIAIARGAEIQDLAIP-------PNSSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQSRGRLEA 813
            + QE +PS +         Q PSL V   D+ P       WE NY+EAAI+L+EG NN+KF  HP+   AL AYL VHN++F+  +L  AL+L+ L   E+P    L + T++H+++EL AL+++  +  +K RW+G+  F +HKRT++K S LVV  +EA+V+LVR  +H RV+RALR IFL+D  YCGGVRR +RQI QSLPP +D+L+L+ F M+I+++ GFYLF        F  L+   ++LFVLLTTAN+PDVMM SY+++ WS +FF+ YLSI LYF+MNL+LAVV+D F  IEK KF+ L LHKR A QHA++LLV++ RP  +S++ FEGL+R Y+   S  E +L F+ LN+SNT  L + +FY+I+      W   +  + WF         +   I  +V S  F+Y + ++V  NG+ ++V+T  +   +  S    + PW    F+ +Y +E  +K+ G+G   Y SS WN+FDF+VT    L L+   L++ P +++V+LRPL+LL LF++KKR+R+V  T   L PR+ S  + LL  YY F I+GME F       CC +S+V   + ++  +  N       Y+YLNNF ++  S +TLFELTVVNNWY+IMEG    T    SR++FMTFYI TM+VMTIIVAFILEAF+FR+ Y +  ++E E       +K +  E LL   E+ R A    +++  L            +S  F G +SR K  L   MY +E+ +W  +  R++ Q + R  A
Sbjct:   29 LGQEDLPSKNGGGQSGPNSQVPSL-VSGADSPPSSPPGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPALKLRTYVHATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFNTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYHLLVSQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKKNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFISK---HVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFSGRLSPNCCNSSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTS-HWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSR-KSQESEVDSGIVIEKEMSKEELLAILELHREARGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQQLRGSA 798          

BLAST of two pore channel 1 vs. SwissProt
Match: gi|81881850|sp|Q9EQJ0.1|TPC1_MOUSE (RecName: Full=Two pore calcium channel protein 1; AltName: Full=Voltage-dependent calcium channel protein TPC1)

HSP 1 Score: 581.252 bits (1497), Expect = 0.000e+0
Identity = 324/772 (41.97%), Postives = 471/772 (61.01%), Query Frame = 0

Query:   70 IEQEMIPSTD---------QRPSLSVEDMDAHPQW-MREQWEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGV-VDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHI-PLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANV------YFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRI----AIARGAE---------IQDLAIPPNSSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQSRGR 810
            + QE +PS +         Q PSL V   D+ P       WE NY+EAAI+L+EG NN+KF  HP+   AL AYL VHN++F+  +L  AL+L+ L   E+P    L + T++H+++EL AL+++  +  +K RW+G+  F +HKRT++K S LVV  +EA+V+LVR  +H RV+RALR IFL+D  YCGGVRR +RQI QSLPP +D+L+L+ F M+I+++ GFYLF        F  L+   ++LFVLLTTAN+PDVMM SY+++ WS +FF+ YLSI LYF+MNL+LAVV+D F  IEK KF+ L LHKR A QHA+ LL ++ RP  +S++ FEGL+R Y+   S  E +L F+ LN+SNT  L + +FY+I+      W   +  + WF         +   I  +V S  F+Y + ++V  NG+ ++V+T  +   + TS    + PW    F+ +Y +E  +K+ G+G   Y SS WN+FDF+VT    L L+   L++ P +++V+LRPL+LL LF++KKR+R+V  T   L PR+ S  + LL  YY F I+GME F       CC  S+V   + ++  +  N       Y+YLNNF ++  S +TLFELTVVNNWY+IMEG    T    SR++FMTFYI TM+VMTIIVAFILEAF+FR+ Y +     +    + +E  ++ EE+  +       RG           +  +     +S  F G +SR K  L   MY +E+ +W  +  R++ Q + R
Sbjct:   29 LGQEQLPSKNGGSHSIHNSQVPSL-VSGADSPPSSPTGHNWEMNYQEAAIYLQEGQNNDKFFTHPKDARALAAYLFVHNHFFYMMELLTALLLLLLSLCESPAVPVLKLHTYVHATLELFALMVVVFELCMKLRWLGFHTFVRHKRTMVKTSVLVVQFIEAIVVLVRQTSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSTNPSDPYFSTLENSIVNLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKHKFKSLLLHKRTAIQHAYGLLASQRRPAGISYRQFEGLMRFYKPRMSARERFLTFKALNQSNTPLLSLKDFYDIYEVAALQWKAKRNRQHWFDELPRTAFLIFKGINILVNSKAFQYFMYLVVAVNGVWILVETFMLKGGNFTSK---HVPWSYLVFLTIYGVELFMKVAGLGPVEYLSSGWNLFDFSVTAFAFLGLLALTLNMEPFYFIVVLRPLQLLRLFKLKKRYRNVLDTMFELLPRMASLGLTLLTFYYSFAIVGMEFFNGRLTPNCCNTSTVADAYRFINHTVGNKTKVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTS-HWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKSQDSEVDSGIVIEKEMSKEELMAVLELYREERGTSSDVTRLLDTLSQMEKYQQNSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQKLR 795          

BLAST of two pore channel 1 vs. SwissProt
Match: gi|125991219|sp|Q9ULQ1.3|TPC1_HUMAN (RecName: Full=Two pore calcium channel protein 1; AltName: Full=Voltage-dependent calcium channel protein TPC1)

HSP 1 Score: 565.074 bits (1455), Expect = 0.000e+0
Identity = 317/732 (43.31%), Postives = 457/732 (62.43%), Query Frame = 0

Query:  100 WEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGV-VDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHI-PLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANV------YFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAI------ARGAE---------IQDLAIPPNSSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQSR 808
            WE NY+EAAI+L+EG NN+KF  HP+  +AL AYL  HN+ F+  +L  AL+L+ L   EAP    L +  ++H+++EL AL+++  +  +K RW+G   F +HKRT++K S LVV  +EA+V+LVR  +H RV+RALR IFL+D  YCGGVRR +RQI QSLPP +D+L+L+ F M+I+++ GFYLF        F  L+   +SLFVLLTTAN+PDVMM SY+++ WS +FF+ YLSI LYF+MNL+LAVV+D F  IEK KF+ L LHKR A QHA+ LL+++ RP  +S++ FEGL+R Y+   S  E YL F+ LN++NT  L + +FY+I+      W   +  E WF         +   I  +V S  F+Y + ++V  NG+ ++V+T  +   +  S    + PW    F+ +Y +E  LK+ G+G   Y SS WN+FDF+VTV   L L+   L++ P +++V+LRPL+LL LF++K+R+R+V  T   L PR+ S  + LL+ YY F I+GME F  +    CC  S+V   + +   +  N       Y+YLNNF ++  S +TLFELTVVNNWY+IMEG    T    SR++FMTFYI TM+VMTIIVAFILEAF+FR+ Y +     +    + +E  ++ EE+  +A+      ARGA          +  +      S  F G +SR K  L   MY +E+ +W  +  R++ Q R
Sbjct:   67 WEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSK---HVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTS-HWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEEL--VAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQR 792          

BLAST of two pore channel 1 vs. SwissProt
Match: gi|75166464|sp|Q94KI8.1|TPC1_ARATH (RecName: Full=Two pore calcium channel protein 1; AltName: Full=Calcium channel protein 1; Short=AtCCH1; AltName: Full=Fatty acid oxygenation up-regulated protein 2; AltName: Full=Voltage-dependent calcium channel protein TPC1; Short=AtTPC1)

HSP 1 Score: 171.785 bits (434), Expect = 2.700e-43
Identity = 177/629 (28.14%), Postives = 294/629 (46.74%), Query Frame = 0

Query:  152 LIGLGFFEAP--CQE---PLC----------VP--THIHSSI-ELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDN------HFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGVVDQ--VNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTK---ERPKEVSFQHFEGLVRTYRGI--TSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNY-NVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPW--VSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVT---VLGILSLILEVLHIPLF-------YVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFT-MIVMTIIVAFILEAFLFRIQYKQFFTKEDEK 735
            L+ L FFE P  C++   P C          +P  T+  S I E+  L ++ +       + G + F+  +  L+KV+ +V++ ++ +V  +           FR++  +R I  I +     +R  +  +   L   L++L L    +L  S   F +F    Q    F         +F+L TT+N PDV + +Y  SRWSS+FFV Y+ I +YF+ NL+LAVVYD+F     ++   +   KR+  + AF L+ +    E  K    + FE L   YR +   S  E  LIF  L+ +    +  DEF ++  A    + Q ++    F ++  +Y   L   ++  V S  F Y I  I++ N I +VV+T  +D   S++      PW    + F  +Y LE  LK+   GF+NY+    N FDF VT   V+G  +  +       F       Y+++ R LRL+ L    +R+R    T + L P L   +  +  +   +  IG++ F  +       N+  ++ F   E   A   + L NF       +TLF L V+ NW V ME Y  +T    S  +F++FY+ T ++++ ++VAF+LEAF     + +   +E+EK
Sbjct:   81 LLFLNFFEQPLWCEKNPKPSCKDRDYYYLGELPYLTNAESIIYEVITLAILLVHTFFPISYEGSRIFWTSRLNLVKVACVVILFVDVLVDFLYLSPLAFDFLPFRIAPYVRVIIFILS--IRELRDTLVLLSGMLGTYLNILALWMLFLLFASWIAFVMFEDTQQGLTVFTSYGATLYQMFILFTTSNNPDVWIPAYKSSRWSSVFFVLYVLIGVYFVTNLILAVVYDSFKEQLAKQVSGMDQMKRRMLEKAFGLIDSDKNGEIDKNQCIKLFEQLT-NYRTLPKISKEEFGLIFDELDDTRDFKINKDEFADLCQAIALRF-QKEEVPSLFEHFPQIYHSALSQQLRAFVRSPNFGYAISFILIINFIAVVVETT-LDIEESSAQK----PWQVAEFVFGWIYVLEMALKIYTYGFENYWREGANRFDFLVTWVIVIGETATFITPDENTFFSNGEWIRYLLLARMLRLIRLLMNVQRYRAFIATFITLIPSLMPYLGTIFCVLCIYCSIGVQVFGGL------VNAGNKKLF---ETELAEDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMESYKDLTGTWWSITYFVSFYVITILLLLNLVVAFVLEAF-----FTELDLEEEEK 686          

BLAST of two pore channel 1 vs. SwissProt
Match: gi|125991220|sp|A0JMD4.1|TPC2_DANRE (RecName: Full=Two pore calcium channel protein 2; AltName: Full=Voltage-dependent calcium channel protein TPC2)

HSP 1 Score: 154.836 bits (390), Expect = 1.352e-37
Identity = 177/727 (24.35%), Postives = 324/727 (44.57%), Query Frame = 0

Query:   69 HIEQEMIPSTDQRPSLSVEDMDAHPQWMREQWEDNY-KEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAAL-VLIGLGFFEAPCQ-----------EPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVV-ILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGVVD--------QVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERP----------KEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTV--LGILSLILEVLHIPLFYV-----------VILRPLRLLTLFRMKKRFRDVFGTAVI------LYPRLTSAIIVLLLIYYFFGIIGMECFQDV----NLKECCKNSSVEQY---FSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIV-MTIIVAFILEAFLFRIQYKQFFTKEDEKK 736
            H   E  P + +R S SV D   + +   +   D Y ++A +F+E+ +       H     +L  Y   ++N   +  LG  + V++ L F E P             +P   P  +   IE+  L +  +    K   IGW++F  +K  L  +  +   +++ ++ I +  D + RV R +RP FL+ N     +++ ++ I ++LP I  +++L+   + ++++ G  +F   D        Q  F +L     SL VLLTTAN PDVM+ +Y+ +R  SIFF+ +     Y LMNLM A++Y+ F        +   + +R   + AF +L    R           + V+   F  ++      +   +A  I +   +   G++  ++F  +FN     + +    +  +S+  +        I+ + +  +       + LAN I +          S++  N  Y   ++  F+  Y +E +LK+V  G+K Y S   NIFD  +TV  L I  +I     IP   V            ++R + +L +FR  +   ++   AV+      L   L +   +LL++YY F ++G+  FQ      +      NSS+E     +S   G+   + ++ NNF     S I L+ + VVNNW+V  + YA  T    S V+F+ +++ + ++ + + VA ILE F ++       + ED ++
Sbjct:   27 HYPDEHTPRS-RRLSYSVTDDSCNVE--EDADADLYVQQAVVFIEDAIKYRSIN-HRVDSGSLRLYRWYYSNLCQW-GLGLTIAVVLALAFIERPSSLTYTSDIRVKPKPWEPPCGMTEGIEIVCLCIFILDVTAKGYLIGWEEFRMNKWLLAYLIVITASVIDWMLSISMLCDENLRVRRLIRPFFLLQNS--SLMKKTLKCIKRTLPEIASVILLLALHICLFTMIGMLIFAKSDDPKQNGEWQTYFRNLPKALSSLLVLLTTANNPDVMIPAYSLNRGYSIFFILFSVFGTYLLMNLMTAIIYNQFRGYLLMSVQTSIIRRRLGIRAAFEVLCCPGRGHTSTQAEGHVERVAVSMFLKVMERVHMKSYCRQA--IVKAARRFPDGFISGEDFQRLFNELDKDFVKEHPPKPEYSSSGL------QHIQYVYSHYYISVLGNAVALANVICICTVLVLNAEKSASEKNYFYMEIINCIFILYYLIEMLLKIVAFGWKGYLSYRNNIFDGFLTVLLLAIQIVIFITFKIPYVDVDPVPRHVMALWEMIRLVNMLIVFRFLRIIPEIKLMAVVASTIVDLVKNLRAFAGILLVVYYMFAVLGIWLFQGAISPPSNMSLVSNSSLENITGPYSMECGTFEQLEYWPNNFDDFASSLILLYNIMVVNNWHVFTDAYARYT-TDWSLVYFVVWWLTSSVMWVNLFVALILENFTYKWDRSNGLSVEDVER 737          

BLAST of two pore channel 1 vs. SwissProt
Match: gi|75326539|sp|Q75VR1.1|TPC1A_TOBAC (RecName: Full=Two pore calcium channel protein 1A; AltName: Full=Voltage-dependent calcium channel protein TPC1A; Short=NtTPC1A)

HSP 1 Score: 147.517 bits (371), Expect = 2.453e-35
Identity = 163/620 (26.29%), Postives = 277/620 (44.68%), Query Frame = 0

Query:  150 LVLIGLGFFEAP--CQEPLCVPTHIHSSIELGALVLITIQAILKTRW-------------IGWKDFFKHKRTL---IKVSTLVVMLLEAVV-ILVRSDNH---FRVSRALRPIFLIDNHYCGGVRRFIRQILQS-------LPPILDMLVLVFFVMLIYSVFGFYLFGVVDQ--VNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTR------IEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGI--TSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVT---VLGILSLILEVLHIPLF-------YVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMI-VMTIIVAFILEAF 719
            L L+ L FFE P  C + L    +      LG L  +T    L                 I ++ F  + R+L   +KV  L++++ + VV IL  +D +   FR++  LR +F I N         IR++  S       L   L+++ L    +L  S   +  F    Q    F    T    +FVL TT+N PDV + +Y  SRW  +FFV Y+ + +YF+ NL+LAVVYD+F         +K++ R   L K  +     N     E+   + F+     +  YR +   S  +   IF  L+ +    + +DEF ++  A    + +      + +  N Y       ++  V    FEY I  ++L N + ++++T         ++   +   V + F  LY +E  LK+   GF+NY+    N FDF VT   V+G  +  +    +          Y++I R LRL+ L    +R+R    T   L P L   +  +  I  F+  +G++ F  +       N ++ Q  + + G++    + L NF       +TLF + V+ NW V M+ Y  +T    +  +F++FY+ +++ ++ +IVAF+LEAF
Sbjct:   84 LALVVLNFFEKPLWCSKHLAESCNNRDYYYLGELPFLTGAESLIFEGVTLLLLIIHILFPISYEGFNLYWRSLLNRVKVILLLILVADIVVYILFLADFYYLPFRIAPYLRVVFFILN---------IRELRDSFFILAGMLGTYLNVVALSALFLLFSSWLAYVFFEDTRQGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGVYFVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGHLNEKQCTLLFEE----LNKYRTLPKISGDDFKSIFSELDDTGDFKINLDEFADLCTAIGLRFQKEDSLPIFEACPNFYHSPASEKLRGFVRGATFEYIIVFVLLVNLVAVIIETTL---DIQNNSGQTFWQKVEFTFGWLYVIEMALKVYTYGFENYWRDGQNRFDFVVTWVIVIGETATFVAPDGLTFLSNGEWIRYLLIARMLRLIRLLMHVERYRAFVATFFTLIPSLVPYLGTIFCILCFYCSLGLQIFGGI---VNTGNPNLAQ--TDLAGND----YLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTAWTYAYFVSFYLISVLWLLNLIVAFVLEAF 678          

BLAST of two pore channel 1 vs. SwissProt
Match: gi|75325199|sp|Q6YLX9.1|TPC1_WHEAT (RecName: Full=Two pore calcium channel protein 1; AltName: Full=Voltage-dependent calcium channel protein TPC1; Short=TaTPC1)

HSP 1 Score: 146.362 bits (368), Expect = 6.419e-35
Identity = 130/444 (29.28%), Postives = 214/444 (48.20%), Query Frame = 0

Query:  305 LFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLH----KRKAAQHAFNLLVTKERPKEVSFQHFEGL--VRTYRGI--TSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPW--VSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVL---HIPLF-------YVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFY-IFTMIVMTIIVAFILEAFLFRIQYKQ 727
            +FVL TT+N PDV + +Y   RW S+FF+ Y+ + +YFL NL+LAV+YD+F    KE+F +  +     ++   Q AF+L+ T  R      Q    L  +  YR +  TS  +  LIF  L++S    +  +EF ++ N     + Q +    +   +  Y   LC  +K  V S  FEY I  ++L N + ++++T  +D  +S+S       W  V +F   +Y  E  LK+  +GF  Y+    N FDF +T    +   L       +P         Y+++ R LRL  +    +RFR    T   L   L   + ++  +   +  IG++ F  +       N ++E      E    N  + L NF       +TLF L V+ NW V ME Y  +T    S ++F++FY I  ++++ +IVAF+LEAF   ++ ++
Sbjct:  271 MFVLFTTSNNPDVWVHAYKIPRWYSLFFIVYVLLGVYFLTNLILAVIYDSF----KEQFAKQLVQVDSIRKNILQKAFDLIDTNNRGYLDREQCISLLNELNKYRSLPKTSREDFELIFAELDRSGDFKVTSEEFADLCNTIAIKF-QKEPPPSYLEKFPFYHSPLCGRLKSFVRSRMFEYIIVFVLLINLVAVIIETT-LDIENSSSQET----WQEVEFFLGWIYVAEMALKIFSLGFGAYWMEGQNKFDFVLTWTIFIGETLTFAFPSKLPFLSNGEWIRYLLLGRVLRLTRILLQVQRFRAFVATFFTLMSSLMPYLGIVFCVLCMYCSIGLQIFGGIVY---AGNPTLE------ETDLFNNDYLLFNFNDYPSGMVTLFNLLVMGNWQVWMESYWQLTGTSWSLIYFVSFYLISILLLLNLIVAFVLEAFFAEMELEK 695          

BLAST of two pore channel 1 vs. SwissProt
Match: gi|75324674|sp|Q6S5H8.1|TPC1_HORVU (RecName: Full=Two pore calcium channel protein 1; AltName: Full=Voltage-dependent calcium channel protein TPC1; Short=HvTPC1)

HSP 1 Score: 144.821 bits (364), Expect = 1.949e-34
Identity = 130/444 (29.28%), Postives = 218/444 (49.10%), Query Frame = 0

Query:  305 LFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLH----KRKAAQHAFNLLVTKERPKEVSFQHFEGL--VRTYRGI--TSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPW--VSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVL---HIPLF-------YVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFY-IFTMIVMTIIVAFILEAFLFRIQYKQ 727
            +FVL TT+N PDV + +Y  SRW S+FF+ Y+ + +YFL NL+LAV+YD+F    KE+F +  +     ++   Q AF L+ T  R      Q    L  +  YR +  TS  +  LIF  L++S    +  +EF ++ N     + Q +    +   +  Y   +C  +K  V S  FEY I  ++L N + ++++T  +D  +S+S       W  V +F   +Y  E  LK+  +GF  Y+    N FDF +T    +   L       +P         Y+++ R LRL  +    +RFR    T   L   L   + ++  I   +  +G++ F  +       N ++E+   +   SN  + F  N++ S     +TLF L V+ NW V ME Y  +T    S ++F++FY I  ++++ +IVAF+LEAF   ++ ++
Sbjct:  271 MFVLFTTSNNPDVWVPAYKISRWYSLFFIVYVLLGVYFLTNLILAVIYDSF----KEQFAKQLVQVDAIRKNILQKAFELIDTNTRGYLDREQCISLLNELNKYRSLPKTSREDFELIFAELDRSGDFKVTSEEFADLCNTIAIKF-QKEPPPSYLEKFPFYHSPVCGRLKSFVRSRTFEYIIVFVLLINLVAVIIETT-LDIENSSSQET----WQEVEFFLGWIYVAEMALKIFSLGFGAYWMEGQNKFDFVLTWTIFIGETLTFAFPSKLPFLSNGEWIRYLLLGRVLRLTRILLQVQRFRVFVATFFTLMSSLMPYLGIVFCILCMYCSLGLQIFGGIVY---AGNPTLEETDLF---SNDYLLFNFNDYPS---GMVTLFNLLVMGNWQVWMESYWQLTGSSWSLIYFVSFYLISILLLLNLIVAFVLEAFFAEMELEK 695          

BLAST of two pore channel 1 vs. SwissProt
Match: gi|75326538|sp|Q75VR0.1|TPC1B_TOBAC (RecName: Full=Two pore calcium channel protein 1B; AltName: Full=Voltage-dependent calcium channel protein TPC1B; Short=NtTPC1B)

HSP 1 Score: 142.51 bits (358), Expect = 8.372e-34
Identity = 139/530 (26.23%), Postives = 245/530 (46.23%), Query Frame = 0

Query:  221 VVILVRSDNH---FRVSRALRPIFLIDNHYCGGVRRFIRQILQS-------LPPILDMLVLVFFVMLIYSVFGFYLFGVVDQ--VNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTR------IEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGI--TSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVT---VLGILSLILEVLHIPLF-------YVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMI-VMTIIVAFILEAF 719
            V IL+ +D +   FR++  LR +F I N         IR++  S       L   L+++ L    +L  S   +  F    Q    F    T    +FVL TT+N PDV + +Y  SRW  +FFV Y+ + +YF+ NL+LAVVYD+F         +K++ R   L K  +     N  +  E+   + F+     +  YR +   S  +   IF  L+ +    + ++EF ++ +A    + +      + +  N Y       ++  +    FEY I  ++L N + ++++T         ++   +   V + F  LY +E  LK+   GF+NY+    N FDF VT   V+G  +  +    +          Y++I R LRL+ L    +R+R    T + L P L   +  +  I  F+  +G++ F  +       N ++ Q  + + G++    + L NF       +TLF + V+ NW V M+ Y  +T    +  +F++FY+ +++ ++ +IVAF+LEAF
Sbjct:  174 VYILLPADFYYLPFRIAPYLRVVFFILN---------IRELRDSFFILAGMLGTYLNVVALSALFLLFSSWLAYVFFEDTRQGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGVYFVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGLLNEKQCTLLFEE----LNKYRTLPKISGDDFKSIFNELDDTGDFKINLEEFADLCSAIGLRFQKEDSLPIFEACPNFYHSPASEKLRGFIRGATFEYIIVFVLLVNLVAVIIETTL---DIQNNSGQTFWQKVEFTFGWLYVIEMALKVYTYGFENYWRDGQNRFDFIVTWVIVIGETTTFVAPDDLTFLSNGEWIRYLLIARMLRLIRLLMHVERYRAFVATFLTLIPSLMPYLGTIFCILCFYCSLGLQIFGGI---VNTGNPNLAQ--TDLAGND----YLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTSWTYAYFVSFYLISVLWLLNLIVAFVLEAF 678          

BLAST of two pore channel 1 vs. SwissProt
Match: gi|73621984|sp|Q5QM84.2|TPC1_ORYSJ (RecName: Full=Two pore calcium channel protein 1; AltName: Full=OsTPC1; AltName: Full=Voltage-dependent calcium channel protein TPC1)

HSP 1 Score: 138.272 bits (347), Expect = 2.021e-32
Identity = 147/551 (26.68%), Postives = 262/551 (47.55%), Query Frame = 0

Query:  204 KRTLIKVSTLVVMLLEA-VVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGVV------DQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERP---KEVSFQHFEGLVRTYRGI--TSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHK-LCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPW--VSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIP-----------LFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFY-IFTMIVMTIIVAFILEAFLFRIQYKQ 727
            K T+ K+  L++ +L   +++   S   FRV+  +R  FLI N       R +R    +L  ++   + V  + L++ +F  +L  V        +  F    T    +F+L TT+N PDV + +Y  SRWSS+FF+ Y+ + +YFL NL+LAV+YD+F     ++  +    ++   + AF ++    +    KE      + L + YR +  TS  +  LIF  L++S    +  +EF  + N     + Q +    +   Y  + H  LC ++K  V S  FEY +  ++L N + ++++T  +D  +S+S       W  V + F  +Y +E  LK+  +GF  Y+    N FDF +T    +   L     P           + Y+++ R LRL  +    +RFR    T   L   L   + ++      +  +G++ F  +       N ++E+   +   SN  + F  N++ S     +TLF L V+ NW   ME Y  +T    S ++F++FY I  ++++ +IVAF+LEAF   ++ ++
Sbjct:  181 KNTINKLKVLLLFILACDILVFAFSPQPFRVAPYIRVAFLIMNI------RELRMCAVTLVGMVGTYLNVLALSLLFLLFASWLAYVTFEDTPQGKTVFSSYGTTLYQMFILFTTSNNPDVWVPAYKSSRWSSLFFIVYVLLGVYFLTNLILAVIYDSFKEQLAKQVSQADCTRKSILEKAFGIIDATGQGYLNKEQCLSLLDELNK-YRSLPKTSREDFELIFAELDQSGDFKVTSEEFATLCNTIAIKF-QKEPPPSYLEKYPSFYHSALCEWLKSFVRSPLFEYIVIFVLLMNLVAVIIETT-LDIENSSSQK----VWQEVEFVFGWIYVIEMALKIFSLGFGAYWMEGQNKFDFVLTWTIFIGETL-TFAFPSKLSFLSNGEWIRYLLLGRMLRLTRILLQVRRFRAFVATFFTLMSSLMPYLGIVFCTLCIYCSLGLQIFGGIVY---AGNPTLEETDLF---SNDYLLFNFNDYPS---GMVTLFNLLVMGNWQAWMESYRQLTGSYWSLIYFVSFYLISVLLLLNLIVAFVLEAFFAEMELEK 708          

BLAST of two pore channel 1 vs. nr
Match: gi|1022770066|gb|KZS14480.1| (Two pore calcium channel protein 1 [Daphnia magna])

HSP 1 Score: 690.263 bits (1780), Expect = 0.000e+0
Identity = 347/762 (45.54%), Postives = 501/762 (65.75%), Query Frame = 0

Query:   98 EQWEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGV-VDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNY-NVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAE---------------------------IQDLAIPPNSSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQSRGRLEAAILAESAANNYHAHGDN 830
            + WE NY+EAAIFLEEG+NN+K T HP CHEALPAYL+VHN W++  D  A+L+LI L F E P      +P  +HSS+EL ALV+++++ I+K RW+G+K F  H R+++K  TL++M+ E++V+LVR  +HFRV+RALRPIFL+DNH+ GGVRR++RQ+LQS+PP +DML L+ FV+L YSV GFY+FG  V+   F  LQ  FISLF+LLTTAN+PDVMM SY  +RWSS+FF++YLSI LYFLMNL+LA VY +F+ +E++KF++L LH+RKAAQHAF LL+++     ++ QHF GL+R      +  E YL+FR+LN S++G + ++EFY I++     W    +E  WFS   +  +  +   + R+VT  +F+Y +C+++   GI+++ +TA + S   ++   +   W +Y FV+ Y LEA LK+ G+GF  YFSS WN FDFAVT + ++ L  E+L     ++V+ R  R+L +F+++KR+RDVFGT  IL  RL  A IV L++YY + I+GME F + +L  CCKNS+VE  F +  GS++N Y+YLNNF+++  S +TLFELTV+NNWY+IMEGYA+ T    SR++FM FY+  MI+++I+VA +L+ F+FRI YK+  TK+DEK+ +   V L +EE  R+     AE                             DL      S  FTG + R K+ L   MY DE+  W+  A  +E Q + +   +   E     +   G+ 
Sbjct:   69 KSWELNYREAAIFLEEGINNDKLTSHPSCHEALPAYLLVHNKWYYALDFAASLLLILLTFTEKPANPLFRLPVGVHSSMELMALVIVSVELIMKFRWVGFKIFIHHPRSMVKALTLLIMIAESIVVLVRQSSHFRVTRALRPIFLLDNHHFGGVRRYLRQVLQSVPPAIDMLGLLLFVILFYSVLGFYIFGSNVNDPYFSTLQQAFISLFILLTTANFPDVMMPSYAVTRWSSVFFITYLSIVLYFLMNLLLAAVYASFSSMERKKFQQLLLHRRKAAQHAFRLLLSRSNRGGITLQHFRGLLRNVDPSRTYREMYLMFRLLNTSDSGIINLNEFYHIYDVLDMKWRIRNEEPFWFSRLRSRTLATMAQQVHRLVTWTFFDYFVCLVIFLGGILILTRTAAI-SVHMSNPLELGVTWETYAFVSFYALEACLKMFGMGFHVYFSSGWNCFDFAVTGVSLIGLAAEILG-QWSFLVVARHSRILRVFKLRKRYRDVFGTIFILGQRLLCAGIVFLILYYAYSIVGMELFAEYDLTNCCKNSTVEANFRFENGSSSNGYYYLNNFENVISSYVTLFELTVINNWYIIMEGYAVTTS-QWSRIYFMCFYLTIMILLSIVVASVLDGFMFRISYKEQMTKDDEKRLVEKTVTLNAEEYERLLAVESAEQLSKGKLWHNWFARLRKQEPFRTLEETTLDLPDIRPISVTFTGFRRRTKDVLLSFMYKDEISCWMKQADHEEKQQQPKNSPSKRVEIRQRQHRGSGET 827          

BLAST of two pore channel 1 vs. nr
Match: gi|1238850377|ref|XP_022243091.1| (two pore calcium channel protein 1-like isoform X2 [Limulus polyphemus])

HSP 1 Score: 688.723 bits (1776), Expect = 0.000e+0
Identity = 371/790 (46.96%), Postives = 511/790 (64.68%), Query Frame = 0

Query:   27 GSPRPSYTSDPTSPTLVAAGNIPNCRGCTSRSRRGTGLVAHPHIEQEMIPSTDQRPSLSVEDMDAHPQWMREQWEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLF-GVVDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSAN-----------NIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAEIQDLAIPPNSSFK----FTGEKSRNKEQLQRLMYSDEMDQWLADA 800
            G P+P   SD  +  L     + NC      S   T L               Q P   +E+ D  P  + + W+ NY EAAI+LEEG NN+KF +HPR  +ALPAYL+VHN WF+  DL AA++L+GL   E P      +   IH+S+EL  L ++ I+ ++K RW+G   FFKHKR+LIK+  LV+M++E+VV++ R  NHFR+SRALRP++LIDN++CGG+RR  RQILQSLPPIL+ML L+ F MLI+SV GFYLF        F  LQ  F+SLFVLLTTANYPDVMM +Y  SRWS+IFF+ +L I+LYF+MN+MLAVVY+ FTRIEK+KFR+L LH+RKA QHAF LLV +  P ++SF+HF+GL+  ++      + YL+F+ L+ S T YL +DEFY I+     SW   + E+ WFS ++    ++C+ I+++V S WF Y I ++++ANGI  VV+ + +        +            I   WVS  F+  Y++EA+LK+VG+G   YF S WNI+DF VTVLG+  +I E  ++P  +V+ILRPLRLL LF+M +R+RDV GT  I+ PR  S  +VLL++YYFF IIGME F D N+  CCKN++VEQ++ +   S A  Y+YLNNF+++  S ITLFEL VVNNW++IM+GYA V     SR+FFM FY+ TM+V+ II+AFILEAFLFRIQYK+     D+   +RV+V LT +E+          I  L    ++  +    + G ++R K      MY++E+  WL  A
Sbjct:   45 GIPQPRTFSDSIASLLEIPNRLSNCLN-DGMSSDYTEL---------------QTP---IEESD--PGDLVKAWDMNYHEAAIYLEEGENNDKFDYHPRSKDALPAYLLVHNIWFYSLDLIAAVMLMGLALVEKPAVPGFHLDPGIHASLELLGLFVVGIELVMKLRWMGINPFFKHKRSLIKLVALVLMVIESVVVIARQTNHFRISRALRPVYLIDNYHCGGIRRVTRQILQSLPPILEMLGLLLFFMLIFSVLGFYLFTSNPKDPYFSTLQGSFVSLFVLLTTANYPDVMMPAYAASRWSAIFFIVFLIIHLYFIMNMMLAVVYETFTRIEKDKFRKLLLHRRKACQHAFRLLVNRAHPTQISFRHFQGLMHHFKPKVCKRDVYLMFKTLDTSRTRYLTLDEFYHIYEVGALSWKAQKAEDLWFSEFHSPFLEICSSIQKLVISRWFNYFIVLVIIANGIWQVVEASEISGVGEGKLDTYVVTHHIKSYGIAGTWVSVGFIVAYSVEALLKIVGLGLFGYFQSGWNIYDFTVTVLGLAGVIAEHFNVPFSFVIILRPLRLLRLFKMNRRYRDVLGTVFIVLPRFGSVALVLLIVYYFFAIIGMELFSDFNMINCCKNTTVEQFYHFDNSSLAEGYYYLNNFENIINSCITLFELMVVNNWFIIMDGYAAVA-TEWSRIFFMLFYVVTMVVINIIIAFILEAFLFRIQYKRKMGDMDKDSLVRVDVALTRQELDFCYTHSNLSISQLLHYTHNLTEQYILYRGTRTRTKFSFSLKMYAEEVKCWLQQA 812          

BLAST of two pore channel 1 vs. nr
Match: gi|1238850374|ref|XP_022243090.1| (two pore calcium channel protein 1-like isoform X1 [Limulus polyphemus])

HSP 1 Score: 687.182 bits (1772), Expect = 0.000e+0
Identity = 356/727 (48.97%), Postives = 493/727 (67.81%), Query Frame = 0

Query:   90 DAHPQWMREQWEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLF-GVVDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSAN-----------NIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAEIQDLAIPPNSSFK----FTGEKSRNKEQLQRLMYSDEMDQWLADA 800
            ++ P  + + W+ NY EAAI+LEEG NN+KF +HPR  +ALPAYL+VHN WF+  DL AA++L+GL   E P      +   IH+S+EL  L ++ I+ ++K RW+G   FFKHKR+LIK+  LV+M++E+VV++ R  NHFR+SRALRP++LIDN++CGG+RR  RQILQSLPPIL+ML L+ F MLI+SV GFYLF        F  LQ  F+SLFVLLTTANYPDVMM +Y  SRWS+IFF+ +L I+LYF+MN+MLAVVY+ FTRIEK+KFR+L LH+RKA QHAF LLV +  P ++SF+HF+GL+  ++      + YL+F+ L+ S T YL +DEFY I+     SW   + E+ WFS ++    ++C+ I+++V S WF Y I ++++ANGI  VV+ + +        +            I   WVS  F+  Y++EA+LK+VG+G   YF S WNI+DF VTVLG+  +I E  ++P  +V+ILRPLRLL LF+M +R+RDV GT  I+ PR  S  +VLL++YYFF IIGME F D N+  CCKN++VEQ++ +   S A  Y+YLNNF+++  S ITLFEL VVNNW++IM+GYA V     SR+FFM FY+ TM+V+ II+AFILEAFLFRIQYK+     D+   +RV+V LT +E+          I  L    ++  +    + G ++R K      MY++E+  WL  A
Sbjct:   88 ESDPGDLVKAWDMNYHEAAIYLEEGENNDKFDYHPRSKDALPAYLLVHNIWFYSLDLIAAVMLMGLALVEKPAVPGFHLDPGIHASLELLGLFVVGIELVMKLRWMGINPFFKHKRSLIKLVALVLMVIESVVVIARQTNHFRISRALRPVYLIDNYHCGGIRRVTRQILQSLPPILEMLGLLLFFMLIFSVLGFYLFTSNPKDPYFSTLQGSFVSLFVLLTTANYPDVMMPAYAASRWSAIFFIVFLIIHLYFIMNMMLAVVYETFTRIEKDKFRKLLLHRRKACQHAFRLLVNRAHPTQISFRHFQGLMHHFKPKVCKRDVYLMFKTLDTSRTRYLTLDEFYHIYEVGALSWKAQKAEDLWFSEFHSPFLEICSSIQKLVISRWFNYFIVLVIIANGIWQVVEASEISGVGEGKLDTYVVTHHIKSYGIAGTWVSVGFIVAYSVEALLKIVGLGLFGYFQSGWNIYDFTVTVLGLAGVIAEHFNVPFSFVIILRPLRLLRLFKMNRRYRDVLGTVFIVLPRFGSVALVLLIVYYFFAIIGMELFSDFNMINCCKNTTVEQFYHFDNSSLAEGYYYLNNFENIINSCITLFELMVVNNWFIIMDGYAAVA-TEWSRIFFMLFYVVTMVVINIIIAFILEAFLFRIQYKRKMGDMDKDSLVRVDVALTRQELDFCYTHSNLSISQLLHYTHNLTEQYILYRGTRTRTKFSFSLKMYAEEVKCWLQQA 813          

BLAST of two pore channel 1 vs. nr
Match: gi|1228005120|ref|XP_021934456.1| (two pore calcium channel protein 1-like [Zootermopsis nevadensis])

HSP 1 Score: 687.182 bits (1772), Expect = 0.000e+0
Identity = 381/815 (46.75%), Postives = 509/815 (62.45%), Query Frame = 0

Query:   11 AAPMANSTGNEFTSINGSP---RPSYTSDPTSPTLV-AAGNIPNCRGCTSRSRRGTGLVAHPHIEQEMIPSTDQRPSLSVEDMDAHPQWMREQWEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFG--VVDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLF-YVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSY-VEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAEIQDLAIPP--------NSSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQSRG 809
            +A  ANS  + +T  N        SY S+     +   A + PN  G TS   R                ST+    LS    D H     + WE NY EAAIFLEEG NNEKF  HP   EALPAYL+VHN W++  DL  +L+L+ L F E P      +P  +H SIEL AL +I ++  LK RWIGWK   KHKRT+IK  TL +M +EAV +LVR  +HFRV+RALRPIFL+D  +CGGVRRFIRQI QSLPPI DM+ L+ F + IY+V G++LF     DQ +F  L   F+S+FVLLTTAN+PDVMM SY +S+W SIFFVSYL   LY LMNLMLAVVY+ FTRIE++KFR+L LHKRKA QHAF LLV+K+ P  + F+ FEGL+R Y    SP +  L+F+ LN S +G L ++EFY +++A    W     +  WF      +   C F    VT  +FEY I  ++L+NG+ ++ +    +     SA    A W +  FV L+ +EAVLK++G+G  +YF S WNI+DF+VT+L  + +I+ +  IP F YVV+ RPLRLL LF++KKR+RDVFGT V+L P + S  +V+L++YYFF IIGME F   +++ CC NS+VE ++ Y   GS A  Y+YLN F +L    ITLFELT+VNNW+++M  YA+V     SR +FM FY+FTMIV+TI+VA +LEAF FRIQYK+  +K +E++ L  EV L  EE++         +QD  +            +  F G + RN+E LQR MY  E+++W+ +A   + Q  G
Sbjct:    3 SARGANSVADGYTRFNDDADNIEGSYQSNNIKHEVSQTADDFPNEIGSTSSLNRDI--------------STENSVELSAPG-DTHID-ADQHWEMNYHEAAIFLEEGENNEKFDSHPHNSEALPAYLLVHNQWYYGLDLATSLILLALAFVEEPALPLFKLPVGVHGSIELLALSIIGVELTLKLRWIGWKTILKHKRTMIKCITLGIMFVEAVTVLVRQSSHFRVTRALRPIFLVDTRHCGGVRRFIRQIFQSLPPIFDMMGLLLFFVTIYAVLGYFLFSDDPRDQ-HFTTLHDSFVSMFVLLTTANFPDVMMPSYARSKWFSIFFVSYLCTVLYVLMNLMLAVVYETFTRIERDKFRKLLLHKRKACQHAFKLLVSKQNPSRMRFKQFEGLMRYYAPKKSPRDVVLMFKELNTSGSGSLSLEEFYSVYDAAMLKWEAQFSQIPWFHTTWAPLQTFCQFTHDFVTWKYFEYIIYGLILSNGLTMIFRILEFNDDLQESAVAFSASWDTILFVGLFVVEAVLKILGLGVGHYFGSGWNIYDFSVTLLSAVGVII-LSFIPSFVYVVVFRPLRLLRLFKLKKRYRDVFGTLVLLTPLMCSTAVVMLVLYYFFAIIGMELFAGYDMRNCCVNSTVEDFYKYSANGSTALGYYYLNTFDNLLTGGITLFELTIVNNWFILMNSYALVVS-SWSRAYFMLFYLFTMIVLTIVVASVLEAFRFRIQYKRQTSKREEEQMLHEEVDLKWEEMQ-------VWVQDFQLLEKLRANLVVGGTATFIGCRPRNREVLQRRMYRAEIEEWMKEADLTDKQEHG 791          

BLAST of two pore channel 1 vs. nr
Match: gi|1285001908|ref|XP_023012709.1| (two pore calcium channel protein 1-like isoform X1 [Leptinotarsa decemlineata])

HSP 1 Score: 686.797 bits (1771), Expect = 0.000e+0
Identity = 352/748 (47.06%), Postives = 494/748 (66.04%), Query Frame = 0

Query:   71 EQEMIPSTDQRPSLSVEDMDAHPQWMREQWEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLF-GVVDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEG--SNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAEIQDLAIPPN--------SSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQS 807
            EQ  +     R SL  +D +A      +QWE NY EAAIFLEEG NNEKF  HPR   ALPAYL+VHN+W++ FDL  ++VL+ L   E P  E    P  IH ++EL AL +I ++  LK RWIGW    KHKRT++K  TL++M LEAV +L+R  +HFRV+RALRPIFL+D   CGGVRR+IRQILQSLPPILDML L+ F +  Y++ G++LF G    + F  L   F+S+FVLLTTAN+PDVMM SY+ S+W+++FF+SY+S  LY LMNLMLAVVY+ FT IEK+KFR+L LHKR+A + AF LLV+K+ P  + F+ F GL+R Y   T   +  LIFR LNKSNTG L  DEF  I++A T  W      + WFS     +   C   + +++  +FE+ + +++++NG+ + ++           A    A W +Y F+AL+++EA+ K+VGIG+  Y SS WN+FD +VTVL +    L  +   L  VVILRPLRLL +F++KKR+RD+FGT V+L P + S ++V++++YYFF IIGME F   +L+ CC NS+VE ++ Y     S+   Y+YLNNF ++ IS +TLFELTVVNNW+++M+ YA V+    SR++FM FY+FTM+V+TI+VA +LEAF FRIQYKQ  +K+DE+  L  +V++    +         ++QD+ +  N         +  +TG + R +E LQ  MY+ E+ QWL +A+++E   
Sbjct:   30 EQNPMDEMGSRSSLCRDD-NADT----DQWEMNYHEAAIFLEEGENNEKFDSHPRHPSALPAYLLVHNSWYYGFDLFFSIVLLMLAVVEKPSIENFEFPVGIHGTLELVALGVIGVELALKLRWIGWTTILKHKRTMVKCITLMIMFLEAVTVLIRQSSHFRVTRALRPIFLVDTRACGGVRRYIRQILQSLPPILDMLGLLLFFICSYALLGYFLFSGHKTNLYFQTLGDSFVSMFVLLTTANFPDVMMPSYSISKWTAVFFISYISTVLYVLMNLMLAVVYETFTGIEKDKFRKLLLHKRQACKLAFRLLVSKQTPNNMRFKQFLGLMRYYSPKTKLRDVVLIFRQLNKSNTGLLTQDEFLNIYDAMTLKWRNKTPLDPWFSTAWPPLRVFCRTSRNLISWKYFEHIVYLLIISNGVAMFIRVVENPGTLQDHARLFCASWDTYLFLALFSMEAIFKIVGIGWSEYISSGWNVFDLSVTVLALGGAFLLSMFPELTVVVILRPLRLLRVFKLKKRYRDIFGTLVLLSPLMWSTVVVMMVMYYFFAIIGMELFSGYDLRNCCVNSTVEDFYKYSSNVTSSGIGYYYLNNFSNILISGVTLFELTVVNNWFIVMDAYASVSN-KYSRLYFMQFYLFTMVVLTIVVASVLEAFRFRIQYKQQTSKKDEELMLHEDVVVDWNSLM-------IQVQDVKMLENLQTDFVVGGTVAYTGRRRRTREVLQHHMYAVEIAQWLEEAEQEEKDD 764          

BLAST of two pore channel 1 vs. nr
Match: gi|1285001910|ref|XP_023012711.1| (two pore calcium channel protein 1-like isoform X2 [Leptinotarsa decemlineata])

HSP 1 Score: 683.33 bits (1762), Expect = 0.000e+0
Identity = 345/721 (47.85%), Postives = 484/721 (67.13%), Query Frame = 0

Query:   98 EQWEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLF-GVVDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSYVEG--SNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGLSRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAEIQDLAIPPN--------SSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQS 807
            +QWE NY EAAIFLEEG NNEKF  HPR   ALPAYL+VHN+W++ FDL  ++VL+ L   E P  E    P  IH ++EL AL +I ++  LK RWIGW    KHKRT++K  TL++M LEAV +L+R  +HFRV+RALRPIFL+D   CGGVRR+IRQILQSLPPILDML L+ F +  Y++ G++LF G    + F  L   F+S+FVLLTTAN+PDVMM SY+ S+W+++FF+SY+S  LY LMNLMLAVVY+ FT IEK+KFR+L LHKR+A + AF LLV+K+ P  + F+ F GL+R Y   T   +  LIFR LNKSNTG L  DEF  I++A T  W      + WFS     +   C   + +++  +FE+ + +++++NG+ + ++           A    A W +Y F+AL+++EA+ K+VGIG+  Y SS WN+FD +VTVL +    L  +   L  VVILRPLRLL +F++KKR+RD+FGT V+L P + S ++V++++YYFF IIGME F   +L+ CC NS+VE ++ Y     S+   Y+YLNNF ++ IS +TLFELTVVNNW+++M+ YA V+    SR++FM FY+FTM+V+TI+VA +LEAF FRIQYKQ  +K+DE+  L  +V++    +         ++QD+ +  N         +  +TG + R +E LQ  MY+ E+ QWL +A+++E   
Sbjct:   24 DQWEMNYHEAAIFLEEGENNEKFDSHPRHPSALPAYLLVHNSWYYGFDLFFSIVLLMLAVVEKPSIENFEFPVGIHGTLELVALGVIGVELALKLRWIGWTTILKHKRTMVKCITLMIMFLEAVTVLIRQSSHFRVTRALRPIFLVDTRACGGVRRYIRQILQSLPPILDMLGLLLFFICSYALLGYFLFSGHKTNLYFQTLGDSFVSMFVLLTTANFPDVMMPSYSISKWTAVFFISYISTVLYVLMNLMLAVVYETFTGIEKDKFRKLLLHKRQACKLAFRLLVSKQTPNNMRFKQFLGLMRYYSPKTKLRDVVLIFRQLNKSNTGLLTQDEFLNIYDAMTLKWRNKTPLDPWFSTAWPPLRVFCRTSRNLISWKYFEHIVYLLIISNGVAMFIRVVENPGTLQDHARLFCASWDTYLFLALFSMEAIFKIVGIGWSEYISSGWNVFDLSVTVLALGGAFLLSMFPELTVVVILRPLRLLRVFKLKKRYRDIFGTLVLLSPLMWSTVVVMMVMYYFFAIIGMELFSGYDLRNCCVNSTVEDFYKYSSNVTSSGIGYYYLNNFSNILISGVTLFELTVVNNWFIVMDAYASVSN-KYSRLYFMQFYLFTMVVLTIVVASVLEAFRFRIQYKQQTSKKDEELMLHEDVVVDWNSLM-------IQVQDVKMLENLQTDFVVGGTVAYTGRRRRTREVLQHHMYAVEIAQWLEEAEQEEKDD 736          

BLAST of two pore channel 1 vs. nr
Match: gi|766931539|ref|XP_011497955.1| (PREDICTED: two pore calcium channel protein 1-like isoform X2 [Ceratosolen solmsi marchali])

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 357/785 (45.48%), Postives = 498/785 (63.44%), Query Frame = 0

Query:   46 GNIPNCRGCTSRSR-RGTGLVAHPH-IEQEMIPSTDQRPSLSVEDMDAHPQWMREQ---WEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGVVDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSY-VEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGL-SRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAEIQDLA--------IPPNSSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQSRGRLEAAI 815
            G++ +C    SR + + T  +  P+ +   ++P  D +       +  +P    +    WE NY EAAIFLEEG NNEKF  HPR  E LPAYL+VHN+W++  DL  +LVL+GL F E P      +P  +H SIEL AL+ I I+  LK RWIGW    KHKRT++K  TL +M +E +++LVR  +HFRV+RALRPIFL+D  YCGGVRRFIRQILQ+LPPILDML L+ F +  Y V G+Y+F  +++ +F  LQ  F+SLFVLLTTAN+PDVMM+S+++++W +I+FVSYL   LY +MNLMLAVV + FT  E++KF++LFLHKRKA QHAF LLV+K+ P+++ F+ FEGL+R Y    S  +  L+FR LN S  G L  DEF  +++A   +W        W+      +   C      +   +FE  +  +++ NGI +V +    +     SA+   A W +  F  L+  EA+ K++G+G + Y SS WN+FD   ++L ++      L     + V+ RPLR+L LF++KKR+RDVFGT VIL P + S  IV+L++YYFF IIGME F   +++ CCKN+SVE ++ Y   GS +  Y+YLN F +L  S +TLFELTVVNNW+V+M  YA+   VG+ +R +FMTFY+ TMIV+TI+V+  LEAF FRIQYK+  TK DE+K L  EV L  +E++ I       +QD          IP N+S  F G + R +E LQR MY  E+ +WL++A+   SQ R    +AI
Sbjct:   36 GSMLSCPSSDSRMKMQSTAALDTPNEMGHRILPGMDSQILSESISLSRNPIVTFDPDMYWEMNYHEAAIFLEEGRNNEKFDSHPRHPEDLPAYLLVHNSWYYGLDLFTSLVLLGLAFVEEPAVPLFQLPVWVHGSIELLALITIAIELALKLRWIGWGTMLKHKRTMLKCVTLFIMFIEVLIVLVRQSSHFRVTRALRPIFLVDTKYCGGVRRFIRQILQTLPPILDMLGLLLFFISTYMVLGYYMFFELNR-HFATLQDSFVSLFVLLTTANFPDVMMQSFSKNKWYAIYFVSYLCTMLYVMMNLMLAVVNETFTSAERDKFKKLFLHKRKACQHAFKLLVSKQNPEQMRFKQFEGLMRYYAPHKSIKDTVLMFRYLNISGNGVLSSDEFLNVYDATVLTWEPQYSSVPWYHMTWPPLQIFCQGAHAAIAWPYFETLVYTVIIGNGIAMVCRLIQTEPNREKSAHLFAACWDTLLFGGLFVAEALTKVMGLGIRRYLSSGWNLFDLGTSMLVLIGACGLTLFPTAVFFVVFRPLRMLRLFKIKKRYRDVFGTLVILSPLMCSTAIVMLVLYYFFAIIGMELFAGYDMRNCCKNTSVEDFYKYSANGSTSLGYYYLNTFDNLMASGMTLFELTVVNNWFVLMNAYAVT--VGMYTRAYFMTFYLITMIVLTIVVSSFLEAFRFRIQYKKSTTKRDEEKMLHEEVELKYDELQLI-------VQDFQLIEQIRGIIPVNTSSLFIGSRPRTREVLQRRMYIHEIFEWLSEAK---SQERTNTPSAI 807          

BLAST of two pore channel 1 vs. nr
Match: gi|766931537|ref|XP_011497953.1| (PREDICTED: two pore calcium channel protein 1-like isoform X1 [Ceratosolen solmsi marchali])

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 357/785 (45.48%), Postives = 498/785 (63.44%), Query Frame = 0

Query:   46 GNIPNCRGCTSRSR-RGTGLVAHPH-IEQEMIPSTDQRPSLSVEDMDAHPQWMREQ---WEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGVVDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSY-VEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGL-SRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAEIQDLA--------IPPNSSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQSRGRLEAAI 815
            G++ +C    SR + + T  +  P+ +   ++P  D +       +  +P    +    WE NY EAAIFLEEG NNEKF  HPR  E LPAYL+VHN+W++  DL  +LVL+GL F E P      +P  +H SIEL AL+ I I+  LK RWIGW    KHKRT++K  TL +M +E +++LVR  +HFRV+RALRPIFL+D  YCGGVRRFIRQILQ+LPPILDML L+ F +  Y V G+Y+F  +++ +F  LQ  F+SLFVLLTTAN+PDVMM+S+++++W +I+FVSYL   LY +MNLMLAVV + FT  E++KF++LFLHKRKA QHAF LLV+K+ P+++ F+ FEGL+R Y    S  +  L+FR LN S  G L  DEF  +++A   +W        W+      +   C      +   +FE  +  +++ NGI +V +    +     SA+   A W +  F  L+  EA+ K++G+G + Y SS WN+FD   ++L ++      L     + V+ RPLR+L LF++KKR+RDVFGT VIL P + S  IV+L++YYFF IIGME F   +++ CCKN+SVE ++ Y   GS +  Y+YLN F +L  S +TLFELTVVNNW+V+M  YA+   VG+ +R +FMTFY+ TMIV+TI+V+  LEAF FRIQYK+  TK DE+K L  EV L  +E++ I       +QD          IP N+S  F G + R +E LQR MY  E+ +WL++A+   SQ R    +AI
Sbjct:   39 GSMLSCPSSDSRMKMQSTAALDTPNEMGHRILPGMDSQILSESISLSRNPIVTFDPDMYWEMNYHEAAIFLEEGRNNEKFDSHPRHPEDLPAYLLVHNSWYYGLDLFTSLVLLGLAFVEEPAVPLFQLPVWVHGSIELLALITIAIELALKLRWIGWGTMLKHKRTMLKCVTLFIMFIEVLIVLVRQSSHFRVTRALRPIFLVDTKYCGGVRRFIRQILQTLPPILDMLGLLLFFISTYMVLGYYMFFELNR-HFATLQDSFVSLFVLLTTANFPDVMMQSFSKNKWYAIYFVSYLCTMLYVMMNLMLAVVNETFTSAERDKFKKLFLHKRKACQHAFKLLVSKQNPEQMRFKQFEGLMRYYAPHKSIKDTVLMFRYLNISGNGVLSSDEFLNVYDATVLTWEPQYSSVPWYHMTWPPLQIFCQGAHAAIAWPYFETLVYTVIIGNGIAMVCRLIQTEPNREKSAHLFAACWDTLLFGGLFVAEALTKVMGLGIRRYLSSGWNLFDLGTSMLVLIGACGLTLFPTAVFFVVFRPLRMLRLFKIKKRYRDVFGTLVILSPLMCSTAIVMLVLYYFFAIIGMELFAGYDMRNCCKNTSVEDFYKYSANGSTSLGYYYLNTFDNLMASGMTLFELTVVNNWFVLMNAYAVT--VGMYTRAYFMTFYLITMIVLTIVVSSFLEAFRFRIQYKKSTTKRDEEKMLHEEVELKYDELQLI-------VQDFQLIEQIRGIIPVNTSSLFIGSRPRTREVLQRRMYIHEIFEWLSEAK---SQERTNTPSAI 810          

BLAST of two pore channel 1 vs. nr
Match: gi|766931543|ref|XP_011497957.1| (PREDICTED: two pore calcium channel protein 1-like isoform X4 [Ceratosolen solmsi marchali])

HSP 1 Score: 676.011 bits (1743), Expect = 0.000e+0
Identity = 348/726 (47.93%), Postives = 475/726 (65.43%), Query Frame = 0

Query:  100 WEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGVVDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSY-VEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGL-SRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAEIQDLA--------IPPNSSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQSRGRLEAAI 815
            WE NY EAAIFLEEG NNEKF  HPR  E LPAYL+VHN+W++  DL  +LVL+GL F E P      +P  +H SIEL AL+ I I+  LK RWIGW    KHKRT++K  TL +M +E +++LVR  +HFRV+RALRPIFL+D  YCGGVRRFIRQILQ+LPPILDML L+ F +  Y V G+Y+F  +++ +F  LQ  F+SLFVLLTTAN+PDVMM+S+++++W +I+FVSYL   LY +MNLMLAVV + FT  E++KF++LFLHKRKA QHAF LLV+K+ P+++ F+ FEGL+R Y    S  +  L+FR LN S  G L  DEF  +++A   +W        W+      +   C      +   +FE  +  +++ NGI +V +    +     SA+   A W +  F  L+  EA+ K++G+G + Y SS WN+FD   ++L ++      L     + V+ RPLR+L LF++KKR+RDVFGT VIL P + S  IV+L++YYFF IIGME F   +++ CCKN+SVE ++ Y   GS +  Y+YLN F +L  S +TLFELTVVNNW+V+M  YA+   VG+ +R +FMTFY+ TMIV+TI+V+  LEAF FRIQYK+  TK DE+K L  EV L  +E++ I       +QD          IP N+S  F G + R +E LQR MY  E+ +WL++A+   SQ R    +AI
Sbjct:   84 WEMNYHEAAIFLEEGRNNEKFDSHPRHPEDLPAYLLVHNSWYYGLDLFTSLVLLGLAFVEEPAVPLFQLPVWVHGSIELLALITIAIELALKLRWIGWGTMLKHKRTMLKCVTLFIMFIEVLIVLVRQSSHFRVTRALRPIFLVDTKYCGGVRRFIRQILQTLPPILDMLGLLLFFISTYMVLGYYMFFELNR-HFATLQDSFVSLFVLLTTANFPDVMMQSFSKNKWYAIYFVSYLCTMLYVMMNLMLAVVNETFTSAERDKFKKLFLHKRKACQHAFKLLVSKQNPEQMRFKQFEGLMRYYAPHKSIKDTVLMFRYLNISGNGVLSSDEFLNVYDATVLTWEPQYSSVPWYHMTWPPLQIFCQGAHAAIAWPYFETLVYTVIIGNGIAMVCRLIQTEPNREKSAHLFAACWDTLLFGGLFVAEALTKVMGLGIRRYLSSGWNLFDLGTSMLVLIGACGLTLFPTAVFFVVFRPLRMLRLFKIKKRYRDVFGTLVILSPLMCSTAIVMLVLYYFFAIIGMELFAGYDMRNCCKNTSVEDFYKYSANGSTSLGYYYLNTFDNLMASGMTLFELTVVNNWFVLMNAYAVT--VGMYTRAYFMTFYLITMIVLTIVVSSFLEAFRFRIQYKKSTTKRDEEKMLHEEVELKYDELQLI-------VQDFQLIEQIRGIIPVNTSSLFIGSRPRTREVLQRRMYIHEIFEWLSEAK---SQERTNTPSAI 796          

BLAST of two pore channel 1 vs. nr
Match: gi|766931541|ref|XP_011497956.1| (PREDICTED: two pore calcium channel protein 1-like isoform X3 [Ceratosolen solmsi marchali])

HSP 1 Score: 675.626 bits (1742), Expect = 0.000e+0
Identity = 348/726 (47.93%), Postives = 475/726 (65.43%), Query Frame = 0

Query:  100 WEDNYKEAAIFLEEGLNNEKFTFHPRCHEALPAYLMVHNNWFHFFDLGAALVLIGLGFFEAPCQEPLCVPTHIHSSIELGALVLITIQAILKTRWIGWKDFFKHKRTLIKVSTLVVMLLEAVVILVRSDNHFRVSRALRPIFLIDNHYCGGVRRFIRQILQSLPPILDMLVLVFFVMLIYSVFGFYLFGVVDQVNFGDLQTCFISLFVLLTTANYPDVMMRSYNQSRWSSIFFVSYLSINLYFLMNLMLAVVYDAFTRIEKEKFRRLFLHKRKAAQHAFNLLVTKERPKEVSFQHFEGLVRTYRGITSPTEAYLIFRVLNKSNTGYLQMDEFYEIFNACTYSWSQSQQEEDWFSNYNVYIHKLCTFIKRMVTSNWFEYTICVIVLANGIILVVQTAFMDSPSSTSANNIYAPWVSYFFVALYTLEAVLKLVGIGFKNYFSSYWNIFDFAVTVLGILSLILEVLHIPLFYVVILRPLRLLTLFRMKKRFRDVFGTAVILYPRLTSAIIVLLLIYYFFGIIGMECFQDVNLKECCKNSSVEQYFSY-VEGSNANVYFYLNNFQSLHISAITLFELTVVNNWYVIMEGYAIVTEVGL-SRVFFMTFYIFTMIVMTIIVAFILEAFLFRIQYKQFFTKEDEKKTLRVEVLLTSEEIRRIAIARGAEIQDLA--------IPPNSSFKFTGEKSRNKEQLQRLMYSDEMDQWLADAQRDESQSRGRLEAAI 815
            WE NY EAAIFLEEG NNEKF  HPR  E LPAYL+VHN+W++  DL  +LVL+GL F E P      +P  +H SIEL AL+ I I+  LK RWIGW    KHKRT++K  TL +M +E +++LVR  +HFRV+RALRPIFL+D  YCGGVRRFIRQILQ+LPPILDML L+ F +  Y V G+Y+F  +++ +F  LQ  F+SLFVLLTTAN+PDVMM+S+++++W +I+FVSYL   LY +MNLMLAVV + FT  E++KF++LFLHKRKA QHAF LLV+K+ P+++ F+ FEGL+R Y    S  +  L+FR LN S  G L  DEF  +++A   +W        W+      +   C      +   +FE  +  +++ NGI +V +    +     SA+   A W +  F  L+  EA+ K++G+G + Y SS WN+FD   ++L ++      L     + V+ RPLR+L LF++KKR+RDVFGT VIL P + S  IV+L++YYFF IIGME F   +++ CCKN+SVE ++ Y   GS +  Y+YLN F +L  S +TLFELTVVNNW+V+M  YA+   VG+ +R +FMTFY+ TMIV+TI+V+  LEAF FRIQYK+  TK DE+K L  EV L  +E++ I       +QD          IP N+S  F G + R +E LQR MY  E+ +WL++A+   SQ R    +AI
Sbjct:   87 WEMNYHEAAIFLEEGRNNEKFDSHPRHPEDLPAYLLVHNSWYYGLDLFTSLVLLGLAFVEEPAVPLFQLPVWVHGSIELLALITIAIELALKLRWIGWGTMLKHKRTMLKCVTLFIMFIEVLIVLVRQSSHFRVTRALRPIFLVDTKYCGGVRRFIRQILQTLPPILDMLGLLLFFISTYMVLGYYMFFELNR-HFATLQDSFVSLFVLLTTANFPDVMMQSFSKNKWYAIYFVSYLCTMLYVMMNLMLAVVNETFTSAERDKFKKLFLHKRKACQHAFKLLVSKQNPEQMRFKQFEGLMRYYAPHKSIKDTVLMFRYLNISGNGVLSSDEFLNVYDATVLTWEPQYSSVPWYHMTWPPLQIFCQGAHAAIAWPYFETLVYTVIIGNGIAMVCRLIQTEPNREKSAHLFAACWDTLLFGGLFVAEALTKVMGLGIRRYLSSGWNLFDLGTSMLVLIGACGLTLFPTAVFFVVFRPLRMLRLFKIKKRYRDVFGTLVILSPLMCSTAIVMLVLYYFFAIIGMELFAGYDMRNCCKNTSVEDFYKYSANGSTSLGYYYLNTFDNLMASGMTLFELTVVNNWFVLMNAYAVT--VGMYTRAYFMTFYLITMIVLTIVVSSFLEAFRFRIQYKKSTTKRDEEKMLHEEVELKYDELQLI-------VQDFQLIEQIRGIIPVNTSSLFIGSRPRTREVLQRRMYIHEIFEWLSEAK---SQERTNTPSAI 799