BLAST of pipsqueak vs. L. salmonis genes
Match: EMLSAG00000011119 (supercontig:LSalAtl2s:LSalAtl2s753:172698:206363:1 gene:EMLSAG00000011119 transcript:EMLSAT00000011119 description:"augustus_masked-LSalAtl2s753-processed-gene-1.0")

HSP 1 Score: 88.5817 bits (218), Expect = 1.666e-20
Identity = 43/101 (42.57%), Postives = 67/101 (66.34%), Query Frame = 0

Query:   34 EENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQ 134
            EE+FVDV+L C          S    AHK++L++ S +F+ +LK NPC HP++IL + + ++I ES+L+F+Y GEV I + R++  L+ A  LQI+GL+ Q
Sbjct:   64 EEDFVDVTLAC---------NSQQFNAHKVVLSACSPYFRKLLKSNPCHHPIIILRDVVEIDI-ESLLKFMYNGEVQISEDRIKDFLKTAESLQIRGLAEQ 154          

BLAST of pipsqueak vs. L. salmonis genes
Match: EMLSAG00000007352 (supercontig:LSalAtl2s:LSalAtl2s416:6420:9389:1 gene:EMLSAG00000007352 transcript:EMLSAT00000007352 description:"augustus_masked-LSalAtl2s416-processed-gene-0.4")

HSP 1 Score: 85.8853 bits (211), Expect = 1.242e-18
Identity = 62/169 (36.69%), Postives = 87/169 (51.48%), Query Frame = 0

Query:   31 QLR-EENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQPPTHPDEDGIPEGADPSKVWEQSAEGSTSPSERPESQTSS---HSLKRKSSPKYHFKNKRKPQN 195
            QLR +E+F DV+L C          S  IKAHK+IL++ S FF++++K  P QHP++ L  GI    LES+L F+Y GEV I    L   L  A  L++ GL         +D  P+ ++    +E        P +R  S T     H +KR+SSP  H +NKR   N
Sbjct:   24 QLRNDEDFFDVTLAC---------GSKHIKAHKVILSACSSFFRSLIKSIPHQHPLLYL-RGIDFNHLESVLCFMYNGEVRIKPHELDQFLSIAQELKVNGLM--------QDQSPQSSNGVNKFE------PMPLKRSLSTTEDKPVHEVKRESSPPLHLQNKRIALN 168          

BLAST of pipsqueak vs. L. salmonis genes
Match: EMLSAG00000011532 (supercontig:LSalAtl2s:LSalAtl2s799:336948:340588:1 gene:EMLSAG00000011532 transcript:EMLSAT00000011532 description:"augustus_masked-LSalAtl2s799-processed-gene-3.12")

HSP 1 Score: 85.5001 bits (210), Expect = 3.170e-18
Identity = 47/112 (41.96%), Postives = 73/112 (65.18%), Query Frame = 0

Query:   27 CLDQQLREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGL--SGQPP 136
            CLD    +E F DVS+     C EG+S    I+AHK++LAS S +F  I K+NPC HPV+IL E + ++I ++++ ++Y G VS+ + R+   L+AA +L+I+G+  S  PP
Sbjct:   26 CLDD--LKEXFSDVSI----SCEEGES----IQAHKLVLASCSPYFLKIFKENPCPHPVLILNE-VPLDIFQALMIYMYHGAVSLSEKRVPLFLKAAKHLKIKGIGSSADPP 126          

BLAST of pipsqueak vs. L. salmonis genes
Match: EMLSAG00000008480 (supercontig:LSalAtl2s:LSalAtl2s51:913154:914229:-1 gene:EMLSAG00000008480 transcript:EMLSAT00000008480 description:"maker-LSalAtl2s51-augustus-gene-9.13")

HSP 1 Score: 84.3445 bits (207), Expect = 4.819e-18
Identity = 58/162 (35.80%), Postives = 84/162 (51.85%), Query Frame = 0

Query:   31 QLR-EENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQPPTHPDEDGIPEGADPSKVWEQSAEGSTSPSERPESQTSSHSLKRKSSPKYHFKNKR 191
            QLR +E+F DV+L C          S  IKAHK+IL++ S FF++++K  P QHP++ L  GI    LES+L F+Y GEV I    L   L  A  L++ GL         +D   + ++     E      + P+    + T  H +KR+SSP  H +NKR
Sbjct:   24 QLRNDEDFFDVTLAC---------GSKHIKAHKVILSACSSFFRSLIKSIPHQHPLLYL-RGIDFNHLESVLCFMYNGEVRIKPHELDQFLSVAQELKVNGLV--------QDRSSQSSNEVNKLEPMPLKRSLPT---TNDTPVHEVKRESSPPLHLRNKR 164          

BLAST of pipsqueak vs. L. salmonis genes
Match: EMLSAG00000001548 (supercontig:LSalAtl2s:LSalAtl2s126:209647:210033:1 gene:EMLSAG00000001548 transcript:EMLSAT00000001548 description:"augustus_masked-LSalAtl2s126-processed-gene-2.5")

HSP 1 Score: 78.5666 bits (192), Expect = 9.593e-18
Identity = 41/113 (36.28%), Postives = 71/113 (62.83%), Query Frame = 0

Query:   30 QQLREE-NFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQPPTHPDE 141
            + LR+E +F DV+L C         +    KAHKM+L++ S +F+ +L++NP +HP++IL + +  + L +ILEF+Y GEV++   +L + L+ A  L+++GL+  P T   E
Sbjct:   26 KHLRDEKSFTDVTLAC---------DGQTCKAHKMVLSACSPYFKALLEENPAKHPIIILKD-VPFQHLTAILEFMYAGEVNVAQDQLPAFLKTAERLKVKGLAEAPQTIKQE 128          

BLAST of pipsqueak vs. L. salmonis genes
Match: EMLSAG00000003038 (supercontig:LSalAtl2s:LSalAtl2s1728:22097:26590:1 gene:EMLSAG00000003038 transcript:EMLSAT00000003038 description:"maker-LSalAtl2s1728-augustus-gene-0.10")

HSP 1 Score: 85.1149 bits (209), Expect = 9.715e-18
Identity = 39/106 (36.79%), Postives = 68/106 (64.15%), Query Frame = 0

Query:   28 LDQQLREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSG 133
             D  L+ E  VD +LVC         E + ++AHK++L++ S +FQ I   N C+HP+++L +    E  + I++F+YKGE S+P+S+L S+++AA  L+++GL+ 
Sbjct:   21 FDTLLQNEALVDCTLVC---------EDTSVRAHKVVLSACSPYFQKIFTDNVCKHPIIVLKDIKGWEA-QCIVDFMYKGETSVPESQLTSLIKAAESLKVRGLTS 116          

BLAST of pipsqueak vs. L. salmonis genes
Match: EMLSAG00000006827 (supercontig:LSalAtl2s:LSalAtl2s385:122699:145346:1 gene:EMLSAG00000006827 transcript:EMLSAT00000006827 description:"maker-LSalAtl2s385-augustus-gene-1.13")

HSP 1 Score: 81.6481 bits (200), Expect = 1.546e-16
Identity = 40/101 (39.60%), Postives = 63/101 (62.38%), Query Frame = 0

Query:   34 EENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQ 134
            +E+FVDV+L C         E   IKAHK+IL++ S +F+ + ++ PC HP++IL +      LES++++VY+G+  I    L S L+ A  LQI+GL+ Q
Sbjct:   27 DEDFVDVTLSC---------EGRTIKAHKVILSACSLYFRDVFRETPCNHPIIIL-KDTQYSDLESLVKYVYQGQXYISQENLSSFLKTADALQIKGLAEQ 117          

BLAST of pipsqueak vs. L. salmonis genes
Match: EMLSAG00000001228 (supercontig:LSalAtl2s:LSalAtl2s11:428586:431606:1 gene:EMLSAG00000001228 transcript:EMLSAT00000001228 description:"maker-LSalAtl2s11-augustus-gene-4.13")

HSP 1 Score: 79.337 bits (194), Expect = 2.036e-16
Identity = 55/167 (32.93%), Postives = 83/167 (49.70%), Query Frame = 0

Query:   31 QLRE-ENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQPPTHPDEDGIPEGADPSKVWEQSAEGSTSPSERPESQTSSHSLKRKSSPKYHFKNKRKPQNP 196
            QLR+ E+F DV+L C +R          IKAHK+IL++ S FF++++K  P +HP++ L  G+    LES+L F+Y GEV +    L   L  A  L++ GL                    K  EQ+++   SPS  P    S   LK   + K H   K++ ++P
Sbjct:   24 QLRDDEDFFDVTLACGSRQ---------IKAHKVILSTCSSFFRSLIKSVPHEHPLLYL-RGVDFNHLESVLSFMYNGEVRVEQKELNDFLSVAQELRVNGLVQ-----------------DKNLEQTSDAGESPSGSP----SQKDLKHSQTLKNHEMEKKRKKSP 159          

BLAST of pipsqueak vs. L. salmonis genes
Match: EMLSAG00000010910 (supercontig:LSalAtl2s:LSalAtl2s729:47426:53045:-1 gene:EMLSAG00000010910 transcript:EMLSAT00000010910 description:"maker-LSalAtl2s729-augustus-gene-0.17")

HSP 1 Score: 81.2629 bits (199), Expect = 2.764e-16
Identity = 41/98 (41.84%), Postives = 64/98 (65.31%), Query Frame = 0

Query:   34 EENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGL 131
            +++FV ++L C         E   I +HKM+L++ S +F+ +LK NPC HPV+IL +  S E L +I+ FVY GEVS+  SR++S ++ A  L+I+GL
Sbjct:   26 DKDFVXLTLSC---------EGRKISSHKMLLSACSPYFRGLLKDNPCPHPVIILRQ-TSYEDLVAIIHFVYNGEVSLHQSRVKSFIKTAKSLKIRGL 113          

BLAST of pipsqueak vs. L. salmonis genes
Match: EMLSAG00000005406 (supercontig:LSalAtl2s:LSalAtl2s290:493651:518008:1 gene:EMLSAG00000005406 transcript:EMLSAT00000005406 description:"maker-LSalAtl2s290-augustus-gene-5.21")

HSP 1 Score: 78.9518 bits (193), Expect = 4.301e-16
Identity = 50/132 (37.88%), Postives = 79/132 (59.85%), Query Frame = 0

Query:   15 YHLKNSNFI--LGGCLDQQLREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILK-QNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQ--PPTHPDE 141
            Y LK +N+   +       L  E+FVDVSLV         S+   +KAH+++LA+ S +F+ ILK  +  QHPV++L + +    L+ I+EF+Y GEVS+    L S+L++A  L+++GL+ +  PP  PDE
Sbjct:   17 YCLKWNNYAASVTSTFKNILDGEDFVDVSLVA--------SKGHALKAHRVVLAACSVYFREILKGLSLWQHPVIVLKD-VPFTDLQGIVEFIYHGEVSVDQDALPSLLKSAEILKVKGLTEEDPPPFRPDE 139          

BLAST of pipsqueak vs. SwissProt
Match: gi|73621294|sp|P14083.2|LOV_DROME (RecName: Full=Protein jim lovell; AltName: Full=Protein TKR; AltName: Full=Tyrosine kinase-related; Short=dTKR)

HSP 1 Score: 95.9005 bits (237), Expect = 2.338e-19
Identity = 47/119 (39.50%), Postives = 78/119 (65.55%), Query Frame = 0

Query:   13 SLYHLKNSNFILGGCLDQQLREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGL 131
            SL    + N IL    D  L+ +  VDV+LVC        +E+S I+AHKM+L++ S FFQ +  + PC+HPV++L +     ++++I++F+Y+GE+S+P  RLQ+++QA   LQ++GL
Sbjct:  116 SLRWNNHQNHILR-AFDALLKTKTLVDVTLVC--------AETS-IRAHKMVLSACSPFFQRVFAETPCKHPVIVL-KDFRGWVVQAIVDFMYRGEISVPQQRLQTLIQAGESLQVRGL 223          

BLAST of pipsqueak vs. SwissProt
Match: gi|29428068|sp|Q9W0K7.2|BAB1_DROME (RecName: Full=Protein bric-a-brac 1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.948e-18
Identity = 44/120 (36.67%), Postives = 77/120 (64.17%), Query Frame = 0

Query:   15 YHLKNSNFI--LGGCLDQQLREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLS 132
            + L+ +N+   L    DQ L+ E FVDV+L C  R          +KAHKM+L++ S +FQT+L + PCQHP+VI+   ++   L++I+EF+Y+GE+++   ++  +L+ A  L+++GL+
Sbjct:  102 FCLRWNNYQTNLTTIFDQLLQNECFVDVTLACDGRS---------MKAHKMVLSACSPYFQTLLAETPCQHPIVIM-RDVNWSDLKAIVEFMYRGEINVSQDQIGPLLRIAEMLKVRGLA 211          

BLAST of pipsqueak vs. SwissProt
Match: gi|29428067|sp|Q9W0K4.2|BAB2_DROME (RecName: Full=Protein bric-a-brac 2)

HSP 1 Score: 92.4337 bits (228), Expect = 3.244e-18
Identity = 42/109 (38.53%), Postives = 70/109 (64.22%), Query Frame = 0

Query:   24 LGGCLDQQLREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLS 132
            L    D+ L+ E+FVDV+L C         E   IKAHKM+L++ S +FQ +   NPCQHP++I+   +S   L++++EF+YKGE+++   ++  +L+ A  L+I+GL+
Sbjct:  209 LTNVFDELLQSESFVDVTLSC---------EGHSIKAHKMVLSACSPYFQALFYDNPCQHPIIIM-RDVSWSDLKALVEFMYKGEINVCQDQINPLLKVAETLKIRGLA 307          

BLAST of pipsqueak vs. SwissProt
Match: gi|73621174|sp|Q7KRI2.1|LOLAL_DROME (RecName: Full=Longitudinals lacking protein-like; Short=Lola-like protein; AltName: Full=Protein Batman)

HSP 1 Score: 79.7221 bits (195), Expect = 1.060e-16
Identity = 41/107 (38.32%), Postives = 70/107 (65.42%), Query Frame = 0

Query:   30 QQLREE-NFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQP 135
            + LR+E +F DV+L C         E    KAHKM+L++ S +F+ +L++NP +HP++IL + +S   L++ILEF+Y GEV++   +L + L+ A  L+++GL+  P
Sbjct:   24 RHLRDEKSFTDVTLAC---------EGQTCKAHKMVLSACSPYFKALLEENPSKHPIIILKD-VSYIHLQAILEFMYAGEVNVSQEQLPAFLKTADRLKVKGLAETP 120          

BLAST of pipsqueak vs. SwissProt
Match: gi|13124701|sp|Q01295.2|BRC1_DROME (RecName: Full=Broad-complex core protein isoforms 1/2/3/4/5)

HSP 1 Score: 85.8853 bits (211), Expect = 2.901e-16
Identity = 41/101 (40.59%), Postives = 67/101 (66.34%), Query Frame = 0

Query:   34 EENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQ 134
            +E FVDV+L C     EG S    IKAH+++L++ S +F+ +LK  PC+HPV++L + ++   L +++EF+Y GEV++    LQS L+ A  L++ GL+ Q
Sbjct:   28 DEAFVDVTLAC-----EGRS----IKAHRVVLSACSPYFRELLKSTPCKHPVILL-QDVNFMDLHALVEFIYHGEVNVHQKSLQSFLKTAEVLRVSGLTQQ 118          

BLAST of pipsqueak vs. SwissProt
Match: gi|13123979|sp|Q24206.2|BRC4_DROME (RecName: Full=Broad-complex core protein isoform 6)

HSP 1 Score: 85.5001 bits (210), Expect = 4.556e-16
Identity = 41/101 (40.59%), Postives = 67/101 (66.34%), Query Frame = 0

Query:   34 EENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQ 134
            +E FVDV+L C     EG S    IKAH+++L++ S +F+ +LK  PC+HPV++L + ++   L +++EF+Y GEV++    LQS L+ A  L++ GL+ Q
Sbjct:   28 DEAFVDVTLAC-----EGRS----IKAHRVVLSACSPYFRELLKSTPCKHPVILL-QDVNFMDLHALVEFIYHGEVNVHQKSLQSFLKTAEVLRVSGLTQQ 118          

BLAST of pipsqueak vs. SwissProt
Match: gi|27923726|sp|Q24174.2|ABRU_DROME (RecName: Full=Protein abrupt; AltName: Full=Protein clueless)

HSP 1 Score: 85.5001 bits (210), Expect = 4.630e-16
Identity = 40/99 (40.40%), Postives = 64/99 (64.65%), Query Frame = 0

Query:   34 EENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLS 132
            EE+FVDV+L C  R            AHK++L++ S +F+ +LK NPC+HP+VIL + +  + +E++L F+Y GEV++   +L   L+ A  LQI+GL+
Sbjct:   99 EEDFVDVTLACDERS---------FTAHKVVLSACSPYFRRLLKANPCEHPIVILRD-VRCDDVENLLSFMYNGEVNVSHEQLPDFLKTAHLLQIRGLA 187          

BLAST of pipsqueak vs. SwissProt
Match: gi|83287912|sp|Q08605.2|GAGA_DROME (RecName: Full=Transcription factor GAGA; AltName: Full=Adh transcription factor 2; AltName: Full=GAGA factor; Short=GAF; AltName: Full=Neural conserved at 70F; AltName: Full=Trithorax-like protein)

HSP 1 Score: 84.7297 bits (208), Expect = 4.769e-16
Identity = 48/108 (44.44%), Postives = 68/108 (62.96%), Query Frame = 0

Query:   40 VSLVCLNRCNEGD-------SESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQPPTHPD 140
            VS + L RC+ GD       +      AHK++L ++S F   +LK  PC+HPVV+L  G++   LE++LEFVY+GEVS+  ++L S+LQAA  L IQGL+ Q  T  D
Sbjct:   21 VSAIQLLRCH-GDLVDCTLAAGGRSFPAHKIVLCAASPFLLDLLKNTPCKHPVVML-AGVNANDLEALLEFVYRGEVSVDHAQLPSLLQAAQCLNIQGLAPQTVTKDD 126          

BLAST of pipsqueak vs. SwissProt
Match: gi|20455517|sp|P17789.2|TTKB_DROME (RecName: Full=Protein tramtrack, beta isoform; AltName: Full=Repressor protein fushi tarazu; AltName: Full=Tramtrack p69)

HSP 1 Score: 80.4925 bits (197), Expect = 1.109e-14
Identity = 40/105 (38.10%), Postives = 64/105 (60.95%), Query Frame = 0

Query:   28 LDQQLREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLS 132
             DQ L  E F DV+L           E   +KAHKM+L++ S +F T+   +P +HP+VIL + +    ++S+L+F+Y+GEVS+   RL + L+ A  L+I+GL+
Sbjct:   23 FDQLLHAETFTDVTLAV---------EGQHLKAHKMVLSACSPYFNTLFVSHPEKHPIVILKD-VPYSDMKSLLDFMYRGEVSVDQERLTAFLRVAESLRIKGLT 117          

BLAST of pipsqueak vs. SwissProt
Match: gi|47117851|sp|P42282.3|TTKA_DROME (RecName: Full=Protein tramtrack, alpha isoform; AltName: Full=Repressor protein fushi tarazu; AltName: Full=Tramtrack p88)

HSP 1 Score: 80.8777 bits (198), Expect = 1.233e-14
Identity = 41/109 (37.61%), Postives = 65/109 (59.63%), Query Frame = 0

Query:   24 LGGCLDQQLREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGLS 132
            L    DQ L  E F DV+L           E   +KAHKM+L++ S +F T+   +P +HP+VIL + +    ++S+L+F+Y+GEVS+   RL + L+ A  L+I+GL+
Sbjct:   19 LLSVFDQLLHAETFTDVTLAV---------EGQHLKAHKMVLSACSPYFNTLFVSHPEKHPIVILKD-VPYSDMKSLLDFMYRGEVSVDQERLTAFLRVAESLRIKGLT 117          

BLAST of pipsqueak vs. nr
Match: gi|1218237453|ref|XP_021699966.1| (mastermind-like protein 2 isoform X6 [Aedes aegypti])

HSP 1 Score: 286.96 bits (733), Expect = 2.125e-84
Identity = 138/217 (63.59%), Postives = 175/217 (80.65%), Query Frame = 0

Query:  298 QTPSPPDTKSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGIATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELSKAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINRLRKENP 513
            +TPS P  K++T +D+  AL+AL+T  MSL++AS  YGIP+TTLWQRA+R+GI TPK+E  +K W+ + L+SAL+ALR   ISANKASK +GIPSSTLYKIAR+EGI LA PFNA  T+WS EDL +AL+AIR G  +VQKA++EFGIP+GTLYGRCK+ GIELS++    WSE  M++ALESV+ G+MSINQAAIH+NLPYSSLYGR  R + + P
Sbjct:  308 KTPSGP--KTWTAEDMESALEALRTHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPSKTWNEDSLNSALEALRTGTISANKASKAFGIPSSTLYKIARREGIRLAAPFNAAPTTWSAEDLDRALEAIRAGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMMEALESVKQGHMSINQAAIHYNLPYSSLYGRFKRGKYDTP 522          

BLAST of pipsqueak vs. nr
Match: gi|1140212096|ref|XP_019931908.1| (PREDICTED: uncharacterized protein LOC109622136 [Aedes albopictus])

HSP 1 Score: 277.33 bits (708), Expect = 2.907e-84
Identity = 131/200 (65.50%), Postives = 164/200 (82.00%), Query Frame = 0

Query:  316 ALQALKTRQMSLSRASEVYGIPATTLWQRANRMGIATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELSKAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINRLRKENPT 514
            AL+AL+T  MSL++AS  YGIP+TTLWQRA+R+GI TPK+E  +K W+ + L+SAL+ALR   ISANKASK +GIPSSTLYKIAR+EGI LA PFNA  T+WS EDL +AL+AIR G  +VQKA++EFGIP+GTLYGRCK+ GIELS++    WSE  M++ALESV+ G+MSINQAAIH+NLPYSSLYGR  R + + P+
Sbjct:    4 ALEALRTHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPSKTWNEDSLNSALEALRTGTISANKASKAFGIPSSTLYKIARREGIRLAAPFNAAPTTWSAEDLDRALEAIRAGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMMEALESVKQGHMSINQAAIHYNLPYSSLYGRFKRGKYDTPS 203          

HSP 2 Score: 115.161 bits (287), Expect = 4.773e-24
Identity = 61/154 (39.61%), Postives = 103/154 (66.88%), Query Frame = 0

Query:  305 TKSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGI--ATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKK 455
            +K++ +  +  AL+AL+T  +S ++AS+ +GIP++TL++ A R GI  A P   A    WS EDLD AL+A+R  + S  KAS  +GIP+ TLY   ++EGIEL++   +  T WS++ + +AL+++++G M++ +AA  + +P  +LYGR K+
Sbjct:   47 SKTWNEDSLNSALEALRTGTISANKASKAFGIPSSTLYKIARREGIRLAAPFNAAPT-TWSAEDLDRALEAIRAGQTSVQKASTEFGIPTGTLYGRCKREGIELSR---SNPTPWSEDAMMEALESVKQGHMSINQAAIHYNLPYSSLYGRFKR 196          

BLAST of pipsqueak vs. nr
Match: gi|1080045695|ref|XP_018564062.1| (protein tramtrack, beta isoform-like isoform X2 [Anoplophora glabripennis])

HSP 1 Score: 282.337 bits (721), Expect = 1.848e-83
Identity = 205/588 (34.86%), Postives = 295/588 (50.17%), Query Frame = 0

Query:   15 YHLKNSNFI--LGGCLDQQLREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQ------------TILKQNPCQHPVVILPE-------GISMEILESIL----EFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQPPTHPDEDGIPEGADPSKVWEQSAEGSTSPSERPESQTSSHSLKRKSSPKY--------------HFKNKRKPQNPKKFGDEATGLSLELAESNHFEYEMKLEVP--------DMEDEVVEEE---NKPMDFT-----------MKAGNDIKVEEEEEEEEPQGLDV-TEVVSPYFGIEAS-------HAS--------SLRPTLVEQVSVHDYDQTPSPPDT-----------------KSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGIATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKGM-AVQKAASEFGIPSGTLYGRCKKVGIELSKAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR 507
            Y L+ +N+   +     Q L+ E FVDV+L C             +KAHK++L++ S +FQ             I+ Q+ C   +  + E        +S   L+SIL    +   KG    PD +  S     S L +    G+    P    + +  +    + +     TS  ERP  +T     + K+ P +              H +++ +P+  K     + GL       +  ++  +   P        DM D         N P+  T           +K   DIK E     ++   LD+ + +  P   +++        H S         + P +  ++ V+  DQ P   +                  K++T++D+  AL AL+   MSL++AS  YGIP+TTLWQRA+R+GI TPK+E   K W+ E+L+SAL+ALR   ISANKASK YGIPSSTLYKIAR+EGI LA PFNA  T+W+ EDL KAL++IR G  +VQKA++EFGIP+GTLYGRCK+ GIELS++    WSE  M +ALE+V+ G+MSINQAAIH+NLPYSSLYGR  R
Sbjct:    6 YCLRWNNYQSNMTSVFHQLLQNEAFVDVTLAC---------NDLSLKAHKVVLSACSSYFQKLLLENPCKHPTIIMPQDVCYSDLKFIIEFVYKGEIDVSQAELQSILRTADQLKIKGLCESPDDKENS----ESPLPLYCKGGRKSASPRHFRLADSGNRRAKYRR-----TSLQERPSDETPGGCREGKAQPGHSSDEENDMPVALDEHKRSRHQPEQTKPLNMSSHGLMTGQFGVDTDDFPPEPPAPSAIPVGHVDMADHSPTRSLLTNTPIHRTDISDSTKYTLDIKKEADIKFETLRSYDQDSLLDMESHMSGPDHSMDSHDDCDRGLHPSMMVQQDFVGMMPGVSNRLDVNSEDQVPGTKNNGHNENLQHTPRSHGGGPKTWTQEDMDMALDALRNHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPTKSWTEENLNSALEALRTGTISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWTPEDLEKALESIRTGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEAVRLGHMSINQAAIHYNLPYSSLYGRFKR 575          

HSP 2 Score: 134.806 bits (338), Expect = 1.128e-29
Identity = 74/176 (42.05%), Postives = 109/176 (61.93%), Query Frame = 0

Query:  360 KVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELS---KAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR----LRKENPTQWSGFASDLSLEA 527
            K W+ ED+D ALDALR   +S  KAS  YGIPS+TL++ A + GI+  +     + SW++E+L+ AL+A+R G ++  KA+  +GIPS TLY   ++ GI L+    AA   W+  D+ KALES+++G  S+ +A+    +P  +LYGR  R    L + NPT WS  A   +LEA
Sbjct:  373 KTWTQEDMDMALDALRNHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPTK-SWTEENLNSALEALRTGTISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWTPEDLEKALESIRTGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEA 547          

BLAST of pipsqueak vs. nr
Match: gi|1080045693|ref|XP_018564061.1| (protein tramtrack, beta isoform-like isoform X1 [Anoplophora glabripennis])

HSP 1 Score: 281.952 bits (720), Expect = 3.045e-83
Identity = 206/593 (34.74%), Postives = 294/593 (49.58%), Query Frame = 0

Query:   15 YHLKNSNFI--LGGCLDQQLREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQ------------TILKQNPCQHPVVILPE-------GISMEILESIL----EFVYKGEVSIPDSRLQSVLQAASYLQIQGLSGQPPTHPDEDGIPEGADPSKVWEQSAEGSTSPSERPESQTSSHSLKRKSSPKY--------------HFKNKRKPQNPKKFGDEATGLSLELAESNHFEYEMKLEVP-------------DMEDEVVEEE---NKPMDFT-----------MKAGNDIKVEEEEEEEEPQGLDV-TEVVSPYFGIEAS-------HAS--------SLRPTLVEQVSVHDYDQTPSPPDT-----------------KSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGIATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKGM-AVQKAASEFGIPSGTLYGRCKKVGIELSKAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR 507
            Y L+ +N+   +     Q L+ E FVDV+L C             +KAHK++L++ S +FQ             I+ Q+ C   +  + E        +S   L+SIL    +   KG    PD +  S     S L +    G+    P    + +  +    + +     TS  ERP  +T     + K+ P +              H +++ +P+  K     + GL       + F  +     P             DM D         N P+  T           +K   DIK E     ++   LD+ + +  P   +++        H S         + P +  ++ V+  DQ P   +                  K++T++D+  AL AL+   MSL++AS  YGIP+TTLWQRA+R+GI TPK+E   K W+ E+L+SAL+ALR   ISANKASK YGIPSSTLYKIAR+EGI LA PFNA  T+W+ EDL KAL++IR G  +VQKA++EFGIP+GTLYGRCK+ GIELS++    WSE  M +ALE+V+ G+MSINQAAIH+NLPYSSLYGR  R
Sbjct:    6 YCLRWNNYQSNMTSVFHQLLQNEAFVDVTLAC---------NDLSLKAHKVVLSACSSYFQKLLLENPCKHPTIIMPQDVCYSDLKFIIEFVYKGEIDVSQAELQSILRTADQLKIKGLCESPDDKENS----ESPLPLYCKGGRKSASPRHFRLADSGNRRAKYRR-----TSLQERPSDETPGGCREGKAQPGHSSDEENDMPVALDEHKRSRHQPEQTKPLNMSSHGLMTGQTIFSQFGVDTDDFPPEPPAPSAIPVGHVDMADHSPTRSLLTNTPIHRTDISDSTKYTLDIKKEADIKFETLRSYDQDSLLDMESHMSGPDHSMDSHDDCDRGLHPSMMVQQDFVGMMPGVSNRLDVNSEDQVPGTKNNGHNENLQHTPRSHGGGPKTWTQEDMDMALDALRNHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPTKSWTEENLNSALEALRTGTISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWTPEDLEKALESIRTGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEAVRLGHMSINQAAIHYNLPYSSLYGRFKR 580          

HSP 2 Score: 134.806 bits (338), Expect = 1.143e-29
Identity = 74/176 (42.05%), Postives = 109/176 (61.93%), Query Frame = 0

Query:  360 KVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELS---KAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR----LRKENPTQWSGFASDLSLEA 527
            K W+ ED+D ALDALR   +S  KAS  YGIPS+TL++ A + GI+  +     + SW++E+L+ AL+A+R G ++  KA+  +GIPS TLY   ++ GI L+    AA   W+  D+ KALES+++G  S+ +A+    +P  +LYGR  R    L + NPT WS  A   +LEA
Sbjct:  378 KTWTQEDMDMALDALRNHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPTK-SWTEENLNSALEALRTGTISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWTPEDLEKALESIRTGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEA 552          

BLAST of pipsqueak vs. nr
Match: gi|1227098211|gb|OXU22436.1| (hypothetical protein TSAR_009978, partial [Trichomalopsis sarcophagae])

HSP 1 Score: 280.026 bits (715), Expect = 3.941e-83
Identity = 134/210 (63.81%), Postives = 169/210 (80.48%), Query Frame = 0

Query:  305 TKSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGIATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELSKAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINRLRKENP 513
            +KS+T++D+  AL+AL+   MSL++AS  +GIP+TTLWQRA+R+GI TPK++   K WS+E L++ALDALR   ISANKASK +GIPSSTLYKIAR+EGI LA PFNA  T+WS  DL +AL+AIR G  +VQ+AA+E+GIPSGTLYGRCK+ GIELS++    WSE  M +ALESV+ G+MSINQAAIH+NLPYSSLYGR  R + E P
Sbjct:  317 SKSWTQEDMDAALEALRNHDMSLTKASATFGIPSTTLWQRAHRLGIETPKKDGPTKSWSDESLNNALDALRTGTISANKASKAFGIPSSTLYKIARREGIRLAAPFNASPTTWSPADLDRALEAIRSGQTSVQRAATEYGIPSGTLYGRCKREGIELSRSNPTPWSEDAMTEALESVRLGHMSINQAAIHYNLPYSSLYGRFKRGKYEEP 526          

HSP 2 Score: 137.117 bits (344), Expect = 9.051e-31
Identity = 75/177 (42.37%), Postives = 110/177 (62.15%), Query Frame = 0

Query:  360 KVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELS---KAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR----LRKENPTQWSGFASDLSLEAF 528
            K W+ ED+D+AL+ALR  ++S  KAS  +GIPS+TL++ A + GIE  +  +    SWS E L+ ALDA+R G ++  KA+  FGIPS TLY   ++ GI L+    A+   WS  D+ +ALE+++SG  S+ +AA  + +P  +LYGR  R    L + NPT WS  A   +LE+ 
Sbjct:  318 KSWTQEDMDAALEALRNHDMSLTKASATFGIPSTTLWQRAHRLGIETPKK-DGPTKSWSDESLNNALDALRTGTISANKASKAFGIPSSTLYKIARREGIRLAAPFNASPTTWSPADLDRALEAIRSGQTSVQRAATEYGIPSGTLYGRCKREGIELSRSNPTPWSEDAMTEALESV 493          

BLAST of pipsqueak vs. nr
Match: gi|1330921871|gb|PNF37570.1| (hypothetical protein B7P43_G11958 [Cryptotermes secundus])

HSP 1 Score: 275.404 bits (703), Expect = 5.179e-83
Identity = 130/207 (62.80%), Postives = 168/207 (81.16%), Query Frame = 0

Query:  306 KSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGIATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELSKAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINRLRKE 511
            K+++++D+ +AL AL+   MSL++AS  YGIP+TTLWQRA+RMGI TPK+E   K W+ E+L++AL+ALR   ISANKASK YGIPSSTLYKIAR+EGI LA PFNA  T+WS +DL +AL++IR G  +VQ+A++EFGIP+GTLYGRCK+ GIELS++    WSE  M +ALE+V+ G+MSINQAAIH+NLPYSSLYGR  R + E
Sbjct:  160 KTWSQEDMEQALDALRNHNMSLTKASATYGIPSTTLWQRAHRMGIDTPKKEGPTKSWTEENLNNALEALRTGTISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWSPDDLERALESIRSGATSVQRASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEAVRVGHMSINQAAIHYNLPYSSLYGRFKRGKYE 366          

HSP 2 Score: 135.576 bits (340), Expect = 6.382e-31
Identity = 75/177 (42.37%), Postives = 109/177 (61.58%), Query Frame = 0

Query:  360 KVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELS---KAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR----LRKENPTQWSGFASDLSLEAF 528
            K WS ED++ ALDALR   +S  KAS  YGIPS+TL++ A + GI+  +     + SW++E+L+ AL+A+R G ++  KA+  +GIPS TLY   ++ GI L+    AA   WS  D+ +ALES++SG  S+ +A+    +P  +LYGR  R    L + NPT WS  A   +LEA 
Sbjct:  160 KTWSQEDMEQALDALRNHNMSLTKASATYGIPSTTLWQRAHRMGIDTPKKEGPTK-SWTEENLNNALEALRTGTISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWSPDDLERALESIRSGATSVQRASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEAV 335          

BLAST of pipsqueak vs. nr
Match: gi|478253173|gb|ENN73544.1| (hypothetical protein YQE_09795, partial [Dendroctonus ponderosae])

HSP 1 Score: 275.018 bits (702), Expect = 5.354e-83
Identity = 132/203 (65.02%), Postives = 164/203 (80.79%), Query Frame = 0

Query:  306 KSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGIATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELSKAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR 507
            K++T+ D+  AL ALK   MSL++AS  YGIP+TTLWQRA+R+GI TPK+E   K W+ E+L++AL+ALR   ISANKASK YGIPSSTLYKIAR+EGI LA PFNA  T+W+ EDL KAL++IR G  +VQKA++EFGIP+GTLYGRCK+ GIELS++    WSE  M +ALE+V+ G+MSINQAAIH+NLPYSSLYGR  R
Sbjct:  198 KTWTQDDMDMALDALKNHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPTKSWTEENLNNALEALRTGTISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWTPEDLEKALESIRTGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEAVRLGHMSINQAAIHYNLPYSSLYGRFKR 400          

HSP 2 Score: 132.109 bits (331), Expect = 9.110e-30
Identity = 72/176 (40.91%), Postives = 109/176 (61.93%), Query Frame = 0

Query:  360 KVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELS---KAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR----LRKENPTQWSGFASDLSLEA 527
            K W+ +D+D ALDAL+   +S  KAS  YGIPS+TL++ A + GI+  +     + SW++E+L+ AL+A+R G ++  KA+  +GIPS TLY   ++ GI L+    AA   W+  D+ KALES+++G  S+ +A+    +P  +LYGR  R    L + NPT WS  A   +LEA
Sbjct:  198 KTWTQDDMDMALDALKNHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPTK-SWTEENLNNALEALRTGTISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWTPEDLEKALESIRTGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEA 372          

BLAST of pipsqueak vs. nr
Match: gi|1218237451|ref|XP_021699965.1| (longitudinals lacking protein, isoforms H/M/V isoform X5 [Aedes aegypti])

HSP 1 Score: 286.189 bits (731), Expect = 5.859e-83
Identity = 138/217 (63.59%), Postives = 175/217 (80.65%), Query Frame = 0

Query:  298 QTPSPPDTKSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGIATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELSKAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINRLRKENP 513
            +TPS P  K++T +D+  AL+AL+T  MSL++AS  YGIP+TTLWQRA+R+GI TPK+E  +K W+ + L+SAL+ALR   ISANKASK +GIPSSTLYKIAR+EGI LA PFNA  T+WS EDL +AL+AIR G  +VQKA++EFGIP+GTLYGRCK+ GIELS++    WSE  M++ALESV+ G+MSINQAAIH+NLPYSSLYGR  R + + P
Sbjct:  558 KTPSGP--KTWTAEDMESALEALRTHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPSKTWNEDSLNSALEALRTGTISANKASKAFGIPSSTLYKIARREGIRLAAPFNAAPTTWSAEDLDRALEAIRAGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMMEALESVKQGHMSINQAAIHYNLPYSSLYGRFKRGKYDTP 772          

HSP 2 Score: 98.9821 bits (245), Expect = 7.602e-18
Identity = 44/100 (44.00%), Postives = 67/100 (67.00%), Query Frame = 0

Query:   32 LREENFVDVSLVCLNRCNEGDSESSCIKAHKMILASSSQFFQTILKQNPCQHPVVILPEGISMEILESILEFVYKGEVSIPDSRLQSVLQAASYLQIQGL 131
            L  ++FVDV+L C         E + +KAHK++L++ S +FQ IL  NPC+HP +ILP  I    L+ I+EFVY+GE+ + ++ LQS+L+ A  L+I+GL
Sbjct:   49 LETQSFVDVTLAC---------EYNSLKAHKVVLSACSAYFQKILLDNPCKHPTIILPSDICFSDLQFIIEFVYRGEIDVSEAELQSLLRTAEQLKIKGL 139          

BLAST of pipsqueak vs. nr
Match: gi|546678925|gb|ERL89463.1| (hypothetical protein D910_06829, partial [Dendroctonus ponderosae])

HSP 1 Score: 275.018 bits (702), Expect = 6.249e-83
Identity = 132/203 (65.02%), Postives = 164/203 (80.79%), Query Frame = 0

Query:  306 KSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGIATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELSKAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR 507
            K++T+ D+  AL ALK   MSL++AS  YGIP+TTLWQRA+R+GI TPK+E   K W+ E+L++AL+ALR   ISANKASK YGIPSSTLYKIAR+EGI LA PFNA  T+W+ EDL KAL++IR G  +VQKA++EFGIP+GTLYGRCK+ GIELS++    WSE  M +ALE+V+ G+MSINQAAIH+NLPYSSLYGR  R
Sbjct:  200 KTWTQDDMDMALDALKNHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPTKSWTEENLNNALEALRTGTISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWTPEDLEKALESIRTGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEAVRLGHMSINQAAIHYNLPYSSLYGRFKR 402          

HSP 2 Score: 132.109 bits (331), Expect = 1.046e-29
Identity = 72/176 (40.91%), Postives = 109/176 (61.93%), Query Frame = 0

Query:  360 KVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELS---KAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR----LRKENPTQWSGFASDLSLEA 527
            K W+ +D+D ALDAL+   +S  KAS  YGIPS+TL++ A + GI+  +     + SW++E+L+ AL+A+R G ++  KA+  +GIPS TLY   ++ GI L+    AA   W+  D+ KALES+++G  S+ +A+    +P  +LYGR  R    L + NPT WS  A   +LEA
Sbjct:  200 KTWTQDDMDMALDALKNHNMSLTKASATYGIPSTTLWQRAHRLGIDTPKKEGPTK-SWTEENLNNALEALRTGTISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTTWTPEDLEKALESIRTGQTSVQKASTEFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEA 374          

BLAST of pipsqueak vs. nr
Match: gi|1229712368|ref|XP_022118239.1| (uncharacterized protein LOC110995395 [Pieris rapae] >gi|1229712370|ref|XP_022118241.1| uncharacterized protein LOC110995395 [Pieris rapae] >gi|1229712372|ref|XP_022118242.1| uncharacterized protein LOC110995395 [Pieris rapae])

HSP 1 Score: 275.018 bits (702), Expect = 6.400e-83
Identity = 134/205 (65.37%), Postives = 160/205 (78.05%), Query Frame = 0

Query:  306 KSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGIATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKGMA-VQKAASEFGIPSGTLYGRCKKVGIELSKAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINRLR 509
            K ++  D+ +AL ALK   MSL++AS  YGIP+TTLWQRA+RMGI TPK+E ++K W+ +DL +AL ALR   ISANKASK YGIPSSTLYKIAR+EGI LA PFNA  TSW + DL +ALDAIR G A VQ+AAS FGIP+GTLYGRCK+ GIELS++    WSE  M +ALE+V+ G MSINQAAIH NLPYSSLYGR  R +
Sbjct:  154 KGWSPLDMEKALDALKNHHMSLTKASSTYGIPSTTLWQRAHRMGIDTPKKEGSSKSWTEDDLKAALHALRTGAISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTSWRRADLQRALDAIRCGAASVQRAASHFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEAVRVGQMSINQAAIHFNLPYSSLYGRFKRCK 358          

HSP 2 Score: 127.102 bits (318), Expect = 5.111e-28
Identity = 71/176 (40.34%), Postives = 106/176 (60.23%), Query Frame = 0

Query:  360 KVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELS---KAATVHWSETDMVKALESVQSGNMSINQAAIHHNLPYSSLYGRINR----LRKENPTQWSGFASDLSLEA 527
            K WS  D++ ALDAL+   +S  KAS  YGIPS+TL++ A + GI+  +   + + SW+++DL  AL A+R G ++  KA+  +GIPS TLY   ++ GI L+    AA   W   D+ +AL++++ G  S+ +AA H  +P  +LYGR  R    L + NPT WS  A   +LEA
Sbjct:  154 KGWSPLDMEKALDALKNHHMSLTKASSTYGIPSTTLWQRAHRMGIDTPKKEGSSK-SWTEDDLKAALHALRTGAISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTSWRRADLQRALDAIRCGAASVQRAASHFGIPTGTLYGRCKREGIELSRSNPTPWSEDAMGEALEA 328          

HSP 3 Score: 116.701 bits (291), Expect = 2.286e-24
Identity = 64/161 (39.75%), Postives = 103/161 (63.98%), Query Frame = 0

Query:  305 TKSYTKKDVYEALQALKTRQMSLSRASEVYGIPATTLWQRANRMGI--ATPKREATNKVWSNEDLDSALDALRKKEISANKASKLYGIPSSTLYKIARKEGIELAQPFNAVQTSWSQEDLSKALDAIRKG-MAVQKAASEFGIPSGTLYGRCKKVGIELSK 462
            +KS+T+ D+  AL AL+T  +S ++AS+ YGIP++TL++ A R GI  A P   A    W   DL  ALDA+R    S  +A+  +GIP+ TLY   ++EGIEL++   +  T WS++ + +AL+A+R G M++ +AA  F +P  +LYGR K+   ++++
Sbjct:  207 SKSWTEDDLKAALHALRTGAISANKASKAYGIPSSTLYKIARREGIRLAAPFNAAPTS-WRRADLQRALDAIRCGAASVQRAASHFGIPTGTLYGRCKREGIELSR---SNPTPWSEDAMGEALEAVRVGQMSINQAAIHFNLPYSSLYGRFKRCKYQVTQ 363