BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. L. salmonis genes
Match: EMLSAG00000004919 (supercontig:LSalAtl2s:LSalAtl2s259:1047053:1053819:1 gene:EMLSAG00000004919 transcript:EMLSAT00000004919 description:"maker-LSalAtl2s259-augustus-gene-10.14")

HSP 1 Score: 1045.03 bits (2701), Expect = 0.000e+0
Identity = 561/1055 (53.18%), Postives = 732/1055 (69.38%), Query Frame = 0

Query:    1 MGGKESKACPISSEDALKRVSETERRRLQDAFRRLASHAGSQLSGALTAHAGTLSRHVFLREVLGEGVPSALADRIFSLCGGGPVGGGGPSLASGGRGLNFREVLTALVLITRGTPEEKLKFLFGILASDSHNTHIERSHLMRFLRDCDPSASSEAVQSLFESGDKVNYETFAHWIDEHPRAFSVSEWLLVPNLHLNLVNKLDTPTFYQTLAGVTHLEEQEIVELEKRFWTMAGNSATGQIDLAAITPLVSPPLPLILVDSFFSALDENLDGHIDFKELSCGVSAACRGPELERQKFCFKIFDGDRDGVLNERELAIMCECLVLVRNQTAQGGIPIDVNLMVESIFEKHDPKGAGVLTLQEYLVWTV--DNPFPVDFSRLIFHICHIVLGLRPVSRSDERDIVLGWLEKEERAPLTVGSIWHLINMDWWNHWQAYVSHTPGSPTLPPSSVQSSPSELVTSSGKRAGKRVPTLDSTLAMDSNSGVVGTSYQPLNESGITSGKTVATTTPSASPHPSRRSSQAASSNTKTRPGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYG--GSPALPRQVIRNKAGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLL-EDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLN-VKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKGMNGSCIYAYEFHSTMSRSKLTH----NEQQSFSEIQRN 1045
            MGGKESK  P+SSE+ALKRV+E E+RRLQDAFRRL+S +           +G +S++ F+ +VLG+ VPS LADRI++L           S ++  +GLNFRE+L+ LVLITRGT EEK+K +FG+L S S+   IE+S + R +R+ +       +Q LF   +KV+Y+ F  W++ +P    +S+WLL P  H +LVNKLDTPTFYQTLAG THL+E+EIVELEKRFWT+ G+S +GQIDL  +  +VSPPLP  L   FF ALDEN DGHID KELSCGVS ACRGPELERQKF FK+FD +RDGVLNE E   M   +  V  Q  +     D N    S   K+  KG   LTLQE+LVWT   ++    +FS+L++ +CH+ LGL+P++RS+E DIV GW+E+EE+ P + GSIW+L++M+WW++W +YV+     P L P        +L+  S K          + +A + + GVV T Y  +  S I SG T + ++ S+       S+  +S+N   RPG IDN+ L   +  R++SLT EG +LK   K+ +GKDFEL+P+RLW AL+LWYG  G  +LPRQ I N  G  E+EL P+ VK++KHQV+ RP  + +VV GYSAAA   + +         +PTTTRRY A QA FSRRTTI QI+DFLS+RL  K++DMRLW ++D+  M  L E+E  TLED G +D+  ILVEVRSRDGTWPEEI+SL +  +    +  NN+++ +KGVTGL NLGNTCYMNAA+QCV +T+IL +YF+ NCHL+ELNRTNPLGMKGHIAKRFGDL++D+W  + + IAP KLR TI RY+ HF+G QQQD+QELL+FLLDGLHEDLNRVT+K Y ELKDSDGR D  VAAEAW++H  RNKSI+VDLFH             HESVR DPFT+L+LPLPMESS+H+EVIVI+Q+GS+P KYGLTLDM+SKY+ IR  L+++C+IP  NLILV++        P EEQK+K MNG+CI+AYEF +    SKL+H       Q+FS+IQR+
Sbjct:    1 MGGKESKQFPLSSEEALKRVTEGEKRRLQDAFRRLSSRSNV---------SGLISKYNFVMDVLGDIVPSKLADRIYTL------AVWSSSSSANAKGLNFREILSLLVLITRGTREEKIKMMFGLLTS-SNGVCIEKSEMFRLVREWERDCLPHELQHLFAESEKVSYDRFLIWVENYPDCLGLSKWLLTPTDHFSLVNKLDTPTFYQTLAGXTHLDEEEIVELEKRFWTLTGSSHSGQIDLNLVKNIVSPPLPDDLAAPFFHALDENEDGHIDLKELSCGVSXACRGPELERQKFVFKMFDLNRDGVLNEDEFNSMITLVNEVYLQLNKN----DGN----SFVTKNYGKGTH-LTLQEFLVWTTSCESGHMKEFSKLLYQLCHVCLGLKPLTRSNEGDIVRGWIEREEKLPHSSGSIWNLVSMNWWSNWHSYVNF---HPHLTP--------KLLNKSKKL---------NAVASNHSQGVVATGYSSI--SDINSGSTTSLSSLSSKKSIDE-SNITSSANALPRPGHIDNSNLTIPSPYRISSLTGEGARLKP--KIQQGKDFELVPKRLWEALILWYGDAGVMSLPRQTILNSNGVFEVELYPIAVKIMKHQVVPRPPQIPTVVGGYSAAAIHAAGN---------LPTTTRRYIANQAVFSRRTTINQISDFLSSRLVIKMEDMRLWFYRDDLNMKQLSENECRTLEDLGFRDDDGILVEVRSRDGTWPEEISSLMSGGNKNNFNNNNNNISGIKGVTGLNNLGNTCYMNAAIQCVAHTQILYKYFNNNCHLFELNRTNPLGMKGHIAKRFGDLVRDMWSGEARTIAPIKLRWTIGRYRQHFSGFQQQDSQELLAFLLDGLHEDLNRVTEKSYFELKDSDGRPDDEVAAEAWESHSARNKSIIVDLFHXXXXXXXX-----HESVRXDPFTYLSLPLPMESSVHIEVIVIRQNGSIPTKYGLTLDMDSKYTAIRNHLTKLCRIPAENLILVDVP-------PSEEQKVKNMNGTCIFAYEFKAR-PESKLSHVTTTGNTQTFSDIQRS 983          

HSP 2 Score: 568.54 bits (1464), Expect = 9.161e-176
Identity = 277/483 (57.35%), Postives = 344/483 (71.22%), Query Frame = 0

Query: 1149 VSNDIADCQQGVLVAMHRKMMPQEVYFLSMEKYRPVLFGIPLIVPCNETTTYQDLYKSVWTQVSRLVSPLPPRDSST-NHATDCDDSLRYEYPFNLKTVQKDGSWCSQCSWQLYCRGCPLPCYSEPYKFMSSYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFLAAVPAKTVQRYRALKDGGGTVLLPGTIDEISEPSSLTSSLDLMDDDDEAFSDYTPNGTINGHSPHSHLSNGVDGVRGRKRMESTSLLTHTIKDDQLEDFHQHKLAEGHNPLEIDYNLYSMVCHSGVLAGGHYVSYGRHSDKKWYLQNDSACKPINEDSMDKSTAYLLFYEREGLCKSEYLPKIEAEKSLPDTRDLDEELETDLKKTCFIM 1630
            +S D+ D Q+G++V ++RKM+PQE YFLS +KY+PVLFG+PLI+ C+E T+YQDLYKSVWTQVSRLVSPLPPR+++T NHA DCDDSL+YEYPF LK VQK GSWC  C W  +CRGC +PC ++ Y F SS++AIDWDQTALHLRY S QE+ + ED SV ESL+ ATE ITL KCL+AFTREE+LG +EKYYCS C++HQLA KK Q+W+LPPILV +L     L   + + I ILQIVH KRFHY+NGRWIKSHKIV+FP K F+P  +LAAVP+ T++R++ LK    T   P    + +   S + S+D  DDD    +D   N T+                  R R ESTSL    + DD L DFHQH+L +G  PL+I YN+Y MVCHSGVL GGHY SY +HS+ KWYL NDS CK + E  +DKS+AY+LFYERE L    YLPKI     LP T+DLD+EL+ D KK C +M
Sbjct: 1029 LSKDLGDXQRGIIVGLNRKMIPQESYFLSTQKYQPVLFGLPLIINCDEKTSYQDLYKSVWTQVSRLVSPLPPRETTTQNHALDCDDSLKYEYPFVLKVVQKGGSWCHWCPWHRFCRGCVIPCNTKEYSFASSFIAIDWDQTALHLRYRSTQEKVYDEDYSVQESLKRATEPITLGKCLQAFTREEQLGSEEKYYCSICETHQLAAKKLQIWKLPPILVRILYLEYHLD--RGLFIHILQIVHLKRFHYLNGRWIKSHKIVEFPSKEFEPLDYLAAVPSMTLKRFKHLKLSNSTPDSPLQTLQCNGNLSGSGSIDYDDDDVFETTDEPQNTTVYNR---------------RTRQESTSLNMLPVIDDNLRDFHQHRLMDGSEPLDIKYNMYGMVCHSGVLGGGHYTSYTKHSN-KWYLNNDSTCKEVQESQIDKSSAYMLFYERESLSSENYLPKISG--PLPSTQDLDDELDIDFKKQCSLM 1491          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. L. salmonis genes
Match: EMLSAG00000000409 (supercontig:LSalAtl2s:LSalAtl2s1064:33017:37342:-1 gene:EMLSAG00000000409 transcript:EMLSAT00000000409 description:"maker-LSalAtl2s1064-augustus-gene-0.14")

HSP 1 Score: 240.35 bits (612), Expect = 2.488e-65
Identity = 117/275 (42.55%), Postives = 172/275 (62.55%), Query Frame = 0

Query:  760 GLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGS-VPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKGMNGSCIYAYEFHSTMSRSKLTH 1033
            GL+NLGNTC+MN+ +Q + NT  + EYF+ + ++ ++N+TNPLGMKG IAK FG++IK +W  +   I P   +  + R+ P F+G QQQD+QELL+FLLDGLHEDLNRV  KPY+E+KDS  RAD  VA EAWD +  RN S+++D+FHG  KS + C VC   SV FDPF +L+LPLP+   I ++++++  D S  P  + LT+      +++   LSR  KIP  +  + +I   +     +    I  ++      YE   T + + L +
Sbjct:  328 GLSNLGNTCFMNSIIQGLSNTPPITEYFANDNYIEDINKTNPLGMKGEIAKAFGEMIKLMWSGNYSYIVPRSFKSAVGRFAPQFSGYQQQDSQELLTFLLDGLHEDLNRVKQKPYIEIKDSGDRADEEVALEAWDVYKKRNDSVILDVFHGLLKSTLICPVCSKVSVTFDPFCYLSLPLPVHRDIQIKILLVWYDLSKAPTTFKLTVSKTGTIADLLKVLSRETKIPEDHFKVADIFNNKLHKELLSHNLISEIDRENALIYEVPPTTNENXLCY 602          

HSP 2 Score: 78.9518 bits (193), Expect = 9.234e-15
Identity = 41/104 (39.42%), Postives = 51/104 (49.04%), Query Frame = 0

Query: 1317 ESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFL 1420
            +SI L  CLE +T  E+LGED+ +YC  C  HQ A KKF LW LP ILV                      +  KRF Y      K   +VDFPI G D +P++
Sbjct:  774 QSIKLTDCLELYTTTEKLGEDDAWYCPRCSEHQQATKKFDLWSLPSILV----------------------ISLKRFSYTRNWRDKLDTLVDFPINGLDMSPYI 855          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. L. salmonis genes
Match: EMLSAG00000012308 (supercontig:LSalAtl2s:LSalAtl2s899:27583:29657:1 gene:EMLSAG00000012308 transcript:EMLSAT00000012308 description:"maker-LSalAtl2s899-augustus-gene-0.9")

HSP 1 Score: 132.88 bits (333), Expect = 1.067e-33
Identity = 82/261 (31.42%), Postives = 134/261 (51.34%), Query Frame = 0

Query:  699 NLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNK-----AIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRV-------TDKPYVELKDSDGRAD-LVVAAE----AWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMES 942
            N++ ++ +  E    +D  ++ +E   R+    +E+    + N  RR  F+          GL NLGNTC+MN+ +QC+ ++  L E    N   Y  N          +   F D+I+D+W +  K      + P K +G I ++ P F+G  Q D+QE L +LL+G+H +LNRV         K   E    + +AD L++  E      +N+++R+ S V DLF GQF+S + C  C +ESV F+PF  ++LP+P +S
Sbjct:   71 NIVPNKKVDEEKDVEEDRETVELEEVYREDIVEDEV----DENLARRLGFIK----TDHAKGLRNLGNTCFMNSTIQCLAHSXQLLELIQKN---YFTNSEE----NSDLTDSFADVIRDLWSLGGKNSTRATVDPVKFKGEIGKFAPKFSGYNQHDSQEFLQYLLEGIHNELNRVDPDTRKAESKRIAEQSKEEEKADSLLIPIEYGKKCLNNYLLRDNSFVTDLFMGQFRSTLKCLKCNNESVTFEPFWVVSLPIPPKS 316          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. L. salmonis genes
Match: EMLSAG00000010295 (supercontig:LSalAtl2s:LSalAtl2s682:21651:71350:-1 gene:EMLSAG00000010295 transcript:EMLSAT00000010295 description:"maker-LSalAtl2s682-augustus-gene-0.26")

HSP 1 Score: 138.658 bits (348), Expect = 5.018e-33
Identity = 74/186 (39.78%), Postives = 115/186 (61.83%), Query Frame = 0

Query:  756 KGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQE-LLSFLLDGLHEDLNRVTDKPYV-ELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLP 939
            +G+TGL NLGNTCYMN+ LQC+ N  ++A+YF  N +L +LN+ +    KG IA+ +  L++ +W  + K+I+P  ++  I +Y  +F+G  QQDA E  LSF+L  LH D+N + +K  + E K  +  +    A +AW+  +  +KS +   F+GQ KS + C  C +ES R+D F  L+L LP
Sbjct:  555 RGLTGLKNLGNTCYMNSILQCISNFTVMAKYFLENKYLNDLNKKS--DTKGDIAREYSALLQQMWSGNVKSISPSDMKRVIGKYNSNFSGFDQQDAHEFFLSFML-WLHNDVNEIREKVTLPEQKTENAPSKKAAAKKAWEYELCVDKSFIKQTFYGQRKSTLECPSCHYESSRYDSFMELSLELP 737          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. L. salmonis genes
Match: EMLSAG00000004018 (supercontig:LSalAtl2s:LSalAtl2s215:1121780:1126611:-1 gene:EMLSAG00000004018 transcript:EMLSAT00000004018 description:"maker-LSalAtl2s215-snap-gene-10.17")

HSP 1 Score: 83.9593 bits (206), Expect = 2.241e-16
Identity = 50/137 (36.50%), Postives = 71/137 (51.82%), Query Frame = 0

Query:  757 GVTGLTNLGNTCYMNAALQCVFNTKILAEYFSL----NCHLYELNRTNPLG----MKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRAD 885
            G  GL N+GNTCY+NA LQC+  T+ L +YFS     N  + E    N  G    + G ++  F  L+  VW  +++ + P K +  ++   P F G+ Q D QE L+ LLD LHE+L R   K      DS   A+
Sbjct:  100 GXCGLRNVGNTCYLNAGLQCLLATQPLVQYFSAWKEGNERIGEEKEENNGGGGSDLDGVLSSSFSSLLTQVWKGESRVLKPLKFKEKLSNNHPQFKGSHQHDCQEFLALLLDSLHEELKRXPIKTPASSPDSQSSAE 236          

HSP 2 Score: 78.5666 bits (192), Expect = 1.106e-14
Identity = 40/122 (32.79%), Postives = 68/122 (55.74%), Query Frame = 0

Query:  874 YVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSVPIKYGLTLDMESKYSNIRPRLSRICKIPPAN-LILVEI 994
            Y E  DS    + + A   WD ++  N +++   FHGQFK+KV C VC H SV F+PF +L +PLP   +  ++++ + +  SVPI+  L +       N++ RL  +  +  ++ L +VE+
Sbjct:  372 YQESLDSSKLNETIEADRVWDKYLSDNDTVIARNFHGQFKNKVVCSVCDHVSVTFEPFMYLPVPLPGALTRQVDLVFLGR-ASVPIRVRLNMTRVDNVGNLKTRLKEVLMLEESSKLQIVEV 492          

HSP 3 Score: 66.2402 bits (160), Expect = 5.829e-11
Identity = 48/187 (25.67%), Postives = 81/187 (43.32%), Query Frame = 0

Query: 1241 FNLKTVQKDGSWCSQCSWQLYCRGC---PLPCYSEPYKFMSSYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGR--WIKSHKIVDFPIKGFDPTPFLAA 1422
            + L  V   G  C++C +Q +C GC        S  Y   +  +AI +   ++  +  + +E    E +S    +R   + + L  CL+AF+ +E L E+  ++C  C+ +Q A K   +WR P  L                      I++ KRF Y+ G    +K    V+F +  FD T FL+ 
Sbjct:  667 YTLVLVNATGKKCARCIYQAHCYGCIRLERNSSSRIYLQPNDTIAISFTNESVVQKVNTLREH---ESISENPKIR---DKLCLTDCLQAFSEKEVLDENNPWFCPQCQKNQCATKTLTIWRFPNYL----------------------IIYLKRFVYLPGTAGAVKLETPVEFNLSNFDLTQFLSG 825          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. L. salmonis genes
Match: EMLSAG00000010621 (supercontig:LSalAtl2s:LSalAtl2s705:346421:349290:-1 gene:EMLSAG00000010621 transcript:EMLSAT00000010621 description:"maker-LSalAtl2s705-snap-gene-3.69")

HSP 1 Score: 82.0333 bits (201), Expect = 7.066e-16
Identity = 46/128 (35.94%), Postives = 70/128 (54.69%), Query Frame = 0

Query:  740 NNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKA-IAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDL 866
            ++S ++     + +   G+ GL NLGNTCYMNAALQC+ NT  L EYF ++C  Y    +   G    ++K +  L+ ++W     + + P  +  +I    P F G  QQD+QE L   +D LHE+L
Sbjct:  125 SSSSKKERSYGDQITSDGLMGLANLGNTCYMNAALQCLSNTPSLTEYF-ISC-FYPKAHSPLSGKSNSLSKSYCSLMLEMWTKKRSSYVVPSGVVRSIKAAHPIFQGYLQQDSQEFLRCFMDTLHEEL 250          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. L. salmonis genes
Match: EMLSAG00000008828 (supercontig:LSalAtl2s:LSalAtl2s54:658331:662140:1 gene:EMLSAG00000008828 transcript:EMLSAT00000008828 description:"maker-LSalAtl2s54-snap-gene-6.28")

HSP 1 Score: 77.0258 bits (188), Expect = 1.666e-14
Identity = 61/198 (30.81%), Postives = 91/198 (45.96%), Query Frame = 0

Query:  756 KGVTGLTNLGNTCYMNAALQCVFNT----KILAEYFSLNCHLYELNR----TNPLGMKGHIAKRFGDLIKDVWGVDN------KAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLP 939
            K + GL NLGNTC+M+A LQ + N     ++L +  S +  +    R     N +  +G I     + +K V    N      KA +P  L   I +  P F G QQQDA E L ++LD LH +L  +       L+          +  A       + S+V  +F G  +S+VTC VC   S + DPF  L++ +P
Sbjct:   86 KSLVGLRNLGNTCFMSAVLQSLSNIQEFCRVLKQIPSFDQKIVTSQRESRSKNAISTEGPI---MTEELKKVLVALNAGQETKKAFSPGALFMVIWKVVPRFRGYQQQDAHEFLRYMLDRLHTELLSLLPSDLSHLQQKYSPYSCRTST-AGSRGTTSSHSLVTSIFGGMLQSEVTCLVCNASSKKHDPFLDLSIDIP 279          

HSP 2 Score: 57.3806 bits (137), Expect = 2.350e-8
Identity = 41/144 (28.47%), Postives = 66/144 (45.83%), Query Frame = 0

Query: 1281 LAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATE----SITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFL 1420
            L+ID     + LR     + + +++L+    L++ ++    +  L  CLE F   EEL + E+++C SCK+ Q + KKF + RLP +L           CL           H KRF +      K    V FP+ G D + +L
Sbjct:  274 LSIDIPNKFIQLR---KAKNNIQQNLTNQTKLKSDSKEQNPTCHLHDCLEKFVDVEELADSERFFCGSCKNKQRSTKKFWIRRLPNVL-----------CL-----------HIKRFRWSPYSRTKLDTHVSFPLSGLDMSDYL 392          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. L. salmonis genes
Match: EMLSAG00000012481 (supercontig:LSalAtl2s:LSalAtl2s91:267608:270916:1 gene:EMLSAG00000012481 transcript:EMLSAT00000012481 description:"maker-LSalAtl2s91-snap-gene-2.20")

HSP 1 Score: 73.9442 bits (180), Expect = 1.283e-13
Identity = 69/326 (21.17%), Postives = 128/326 (39.26%), Query Frame = 0

Query: 1281 LAIDWDQTA-------------LHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFLAAVPAKTVQRYRALKDGGGTVLLPGTIDEISEPSSLTSSLDLMDDDDEAFSDYTPNGTINGHSPHSHLSNGVDGVRGRKRMESTSLLTHTIKDDQLEDFHQHKLAEGHNPLEIDYNLYSMVCHSGV-LAGGHYVSYGRH-SDKKWYLQNDSACKPINEDSMDKSTAYLLFYER 1591
            L ++WD  A             +H    +A+E S+ ++ S          S++L++C   +T EE+LG+ + ++C  C   +  +KK  LW +P +LV                      +H KRF   +    K   ++DFP+ GFD TP ++   +        +      V++  +I+++                             N  +P  H          ++ +  +++LT        ED + + L             Y++  H G  L GGHY +  R+ +D  WY  +D   K ++++ +    AY+LFY+R
Sbjct:  118 LVLEWDALAKSQIINDDSAPVEIHSTVAAAEEESYPKEAS----------SVSLQECFSLYTSEEKLGQGDAWFCPECDRKREVVKKMGLWSVPDVLV----------------------IHLKRFRQCSLSTDKLLTLIDFPLDGFDMTPNVSRSSSTNSSTCSTIL--SSQVMVESSINKV---------------------------IANAFNPWRH---------PKRYLARSTVLTQ-------EDNYMYDL-------------YAVCNHHGSDLQGGHYTATCRNPTDGIWYSFDDVRTKSLSKNEVVTKDAYILFYQR 353          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. L. salmonis genes
Match: EMLSAG00000004628 (supercontig:LSalAtl2s:LSalAtl2s240:943485:945205:-1 gene:EMLSAG00000004628 transcript:EMLSAT00000004628 description:"maker-LSalAtl2s240-augustus-gene-9.8")

HSP 1 Score: 72.7886 bits (177), Expect = 3.949e-13
Identity = 61/208 (29.33%), Postives = 88/208 (42.31%), Query Frame = 0

Query:  732 EEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLP 939
            E+I +L  N   +R  F NN     G+ GL NLGNTC+M+  +Q + +T +L +YF  + H       N   M   +A+ F    ++ +      + P  L      +  H AG +QQDA E     LD LH  L     K  V   + D                     IV  +F G+ +S V C+ C   S   DPF  ++L LP
Sbjct:  131 EDIVTLQKNK--KRKGFANN--TTIGLRGLINLGNTCFMSCIIQSLIHTPLLRDYFLSDRHRCMFQGKNDC-MVCEVARXF----QEFYSGSKVPLVPHVLLHMTWTHAHHLAGYEQQDAHEFFIATLDLLHRHL---IHKTTVNPSNCD--------------------CIVDKIFTGKLQSDVVCQNCKGVSTTIDPFWDISLDLP 306          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. L. salmonis genes
Match: EMLSAG00000004748 (supercontig:LSalAtl2s:LSalAtl2s24:993307:1011904:-1 gene:EMLSAG00000004748 transcript:EMLSAT00000004748 description:"maker-LSalAtl2s24-augustus-gene-10.16")

HSP 1 Score: 72.0182 bits (175), Expect = 1.310e-12
Identity = 67/237 (28.27%), Postives = 98/237 (41.35%), Query Frame = 0

Query:  758 VTGLTNLGNTCYMNAALQCV-------------------FNTKILAEYFSLNCHLYELNRTNPLGMK-----GHIAKRFGDLI-------------------KDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLH-EDLNRVTDK--PYVELKDSDGRADL-------VVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPME 941
            V GL+NLGNTC+ NA +QC+                   F T  L +  SLN    +L  + PL +        I + F   I                    +  G  + A  P +L G I+   P F G  Q+D+ ELL  L++G+  E++NR       +  L D   R  L       + +   + NH     +IV  LF G   S + C+VC + S  F+PF  ++LPL  E
Sbjct:  508 VRGLSNLGNTCFFNAVMQCLTQSHPLLHILDLHSQKGAPFKTGKLLDGESLNL---QLEXSGPLXLSLAAFLKEIVELFSFFIYNEPDLTISISLALFHFIEMNSMGGKSGAYNPSQLFGRISHRSPQFRGFHQEDSHELLRHLMEGVKTEEVNRQKTSVLKHYGLADKASRVSLHEKTRKRLQSCNRYTNH-----TIVDQLFGGHLVSTILCEVCHNSSQIFEPFLDISLPLIEE 736          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. SwissProt
Match: gi|47606649|sp|Q8NFA0.1|UBP32_HUMAN (RecName: Full=Ubiquitin carboxyl-terminal hydrolase 32; AltName: Full=Deubiquitinating enzyme 32; AltName: Full=Renal carcinoma antigen NY-REN-60; AltName: Full=Ubiquitin thioesterase 32; AltName: Full=Ubiquitin-specific-processing protease 32; Flags: Precursor)

HSP 1 Score: 860.136 bits (2221), Expect = 0.000e+0
Identity = 456/1060 (43.02%), Postives = 643/1060 (60.66%), Query Frame = 0

Query:    1 MGGKESKACPISSEDALKRVSETERRRLQDAFRRLASHAGSQLSGALTAHAGTLSRHVFLREVLGEGVPSALADRIFSLCGGGPVGGGGPSLASGGRGLNFREVLTALVLITRGTPEEKLKFLFGILASDSHNTHIERSHLMRFLRDCDPSASSEAVQSLFESGDKVNYETFAHWIDEHPRAFSVSEWLLVPNLHLNLVNKLDTPTFYQTLAGVTHLEEQEIVELEKRFWTMAGNSATGQIDLAAITPLVSPPLPLILVDSFFSALDENLDGHIDFKELSCGVSAACRGPELERQKFCFKIFDGDRDGVLNERELAIMCECLVLVRNQTAQGGIP---IDVNLMVESIFEKHDPKGAGVLTLQEYLVWTVDNPFPVDFSRLIFHICHIVLGLRPVSRSDERDIVLGWLEKEERAPLTVGSIWHLINMDWWNHWQAYVSHTPGSPTLPPSSVQSSPSELVTSSGKRAGKRVPTLDSTLA-MDSNSGVVGTSYQPLNESGITSGKT-VATTTPSASPHPSRRSSQAASSN--------------TKTRPGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYGGSPALPRQVIRNKAGEV-ELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKGMNGSCIYAYEFHSTMSRSKLTHNEQQSFS 1040
            MG KES+   +S E+AL+RV++ E +RL+DAF+R         +  L+ + G   +H F+REVLG+GVP  +A+ I+   GG              +GL+F  ++  LVL+TRG  EEK K++F + +S+S N ++ R  + R L   D     + ++  F  G+KVNYE F +W+  +  AF+ S WLL   +++ L +  DTPTFYQTLAGVTHLEE +I++LEKR+W +   S TG+ DL    PLVSPP+   L +  F+A DEN D HIDFKE+SCG+SA CRGP  ERQKFCFK+FD DRDGVL+  EL  M   L+ V        IP   +D++ +VE I   HD    G LTL++Y +W+V N    +F  L+F +CHIVLGLRP +  +E  I+ GWLE+E R  L  G  W +I+M WW  W+ YV +      + PSSV +       ++     +    + S+L+ +++       +    +E+  T+G   + + TP A    +R+ + + ++N                 +PG IDN  LV     + TSLT EGG+LK   +L  G+D+E++PE +WRAL  WYG + ALPR VI+N   ++ ELEL P  +  L+ Q  TR    +  V               N G   +     +R  AY   FSR  TI++I ++LS RL  K +DMRLW +  E  + LL+DE   LE   ++DE  +++EVR++D +WPEE++ + N++   R          KG TGL+NLGNTC+MN+++QCV NT+ L +YF    HLYELNRTNP+GMKGH+AK +GDL++++W    K +AP KLR TIA+Y P F G QQQD+QELL+FLLDGLHEDLNRV +KPYVELKDSDGR D  VAAEAWDNH+ RN+SIVVDLFHGQ +S+V CK CGH SVRFDPF FL+LPLPM+S +HLE+ VIK DG+ P++YGL L+M+ KY+ ++ +LS +C +    ++L E+     +  P + QK++      + A+E    +S    +   Q  FS
Sbjct:    1 MGAKESRIGFLSYEEALRRVTDVELKRLKDAFKR---------TCGLSYYMG---QHCFIREVLGDGVPPKVAEVIYCSFGGT------------SKGLHFNNLIVGLVLLTRGKDEEKAKYIFSLFSSESGN-YVIREEMERMLHVVDGKVP-DTLRKCFSEGEKVNYEKFRNWLFLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDVDRDGVLSRVELRDMVVALLEVWKDNRTDDIPELHMDLSDIVEGILNAHDTTKMGHLTLEDYQIWSVKNVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQAGHNWFIISMQWWQQWKEYVKYDANPVVIEPSSVLNGGKYSFGTAAHPMEQVEDRIGSSLSYVNTTEEKFSDNISTASEASETAGSGFLYSATPGADVCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGANLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWV---------------NMGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHKLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTE----KGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYGLRLNMDEKYTGLKKQLSDLCGLNSEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPVPVSPISASSPTQTDFS 1015          

HSP 2 Score: 307.76 bits (787), Expect = 1.352e-83
Identity = 156/311 (50.16%), Postives = 193/311 (62.06%), Query Frame = 0

Query: 1159 GVLVAMHRKMMPQEVYFLSMEKYRPVLFGIPLIVPCNETTTYQDLYKSVWTQVSRLVSPLPPRDSSTNHATDCDDSLRYEYPFNLKTVQKDGSWCSQCSWQLYCRGCPLPCYSEPYKFMSSYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFLAAVPAKTVQRYRALKDGGGTVLLPGTIDEISEPSSLTSSLDLMDDDDEA 1469
            G ++A+HRKMM  E+YFLS +K RP LFG+PLIVPC   T  +DLY +VW QVSRL SPLPP+++S NHA DCDDS+ Y+YPF L+ VQKDG+ C+ C W  +CRGC + C  +     ++Y+A+DWD TALHLRY ++QER   E  SV +S RA  E I L  CL AFT EEELGE+E YYCS CK+H LA KK  LWRLPPIL                      I+H KRF +VNGRWIKS KIV FP + FDP+ FL        Q ++ L   G         DE+SEP  L   +  +D    A
Sbjct: 1074 GYIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSRLASPLPPQEAS-NHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIGNAYIAVDWDPTALHLRYQTSQERVVDEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRLPPIL----------------------IIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLVPRDPALCQ-HKPLTPQG---------DELSEPRILAREVKKVDAQSSA 1351          

HSP 3 Score: 111.309 bits (277), Expect = 4.944e-23
Identity = 45/95 (47.37%), Postives = 71/95 (74.74%), Query Frame = 0

Query: 1535 YNLYSMVCHSGVLAGGHYVSYGRHSDKKWYLQNDSACKPINEDSMDKSTAYLLFYEREGLCKSEYLPKIEAEKSLPDTRDLDEELETDLKKTCFI 1629
            YNLY++ CHSG+L GGHYV+Y ++ + KWY  NDS+CK ++ D +D  +AY+LFYE++G+  +++LPK + +K + DT  +DE+ E+D KK C +
Sbjct: 1510 YNLYAISCHSGILGGGHYVTYAKNPNCKWYCYNDSSCKELHPDEIDTDSAYILFYEQQGIDYAQFLPKTDGKK-MADTSSMDEDFESDYKKYCVL 1603          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. SwissProt
Match: gi|1270743967|sp|M9PD06.1|UBP32_DROME (RecName: Full=Ubiquitin carboxyl-terminal hydrolase 32)

HSP 1 Score: 753.051 bits (1943), Expect = 0.000e+0
Identity = 427/1002 (42.61%), Postives = 589/1002 (58.78%), Query Frame = 0

Query:   93 ASGG--RGLNFREVLTALVLITRGTPEEKLKFLFGILASDSHNTHIERSHLMRFLRDCDPSASSEAVQSLFESGDKVNYETFAHWIDEHPRAFSVSEWLLVPNLHLNLVNKLDTPTFYQTLAGVTHLEEQEIVELEKRFWTMAGNSATGQIDLAAITPLVSPPLPLILVDSFFSALDENLDGHIDFKELSCGVSAACRGPELERQKFCFKIFDGDRDGVLNERELAIMCECLVLV--RNQTAQGGIPIDVNLMVESIFE---KHDPKGAGV--------LTLQEYLVWTV--DNPFPVDFSRLIFHICHIVLGLRPVSRSDERDIVLGWLEKEERAPLTVGSIWHLINMDWWNHWQAYVSHTPGSPTLPPSSVQSSPSELVTSSGKRAGKRVPTLDSTLAMDSNSGVVGTSYQPLNESGITSGKTVATTTPSASPHPSRRSSQAASSNTKTRPGMIDNACLVQGNVSR-VTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYGGSPALPRQVIRNKAGEVELELNPLNVKLLKHQVITRPANV----------SSVVAGYSAAAATISASSTNSGYGSTMPTTT----RRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKD-ETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSL----CNNNS----DRR----SSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKGMNGSCIYAYEF----HSTMSRSKLTHNEQQSFSEIQRN 1045
            A GG  RG++F ++L  LVLITRGT  EK KFL+ +  +D+  T I +S  +R +      A  E+V SLF   ++VN+E F  WI +H  A  +S+WLL  N  ++L ++L+TPTFYQ+LAGVTHLEE++I +LEK FW +   S  GQIDL  + PL+SPP+P   +   F+A DEN DGHIDFKEL CGVSAACRGP +ER +FCFKIFD DRDGVL+  E   M   L+LV   NQ +Q    +   L++  + E   +  P G           LT +++++WTV  D         LIF +CHIV GL P  +  E DIV GWL +EER P  VG  W+LI  DWW  W  Y  HT  +                    KR   +   +D  L  D        S+   +     S    + T  ++    +     A       RPG IDN+ L+  N  R V +LT EGG LK +  L +  DFEL+P+ LW+AL  WYG +  LPRQVI+    +VELEL PLN+++L HQ       V          S+V  GY   A       +  GY +   ++     +RY AY AAFSR  T+RQ+ +FL  +L  K +D+RLW     +    LLE++A+ L++  ++D   +L+E+R++D TWPEE+ SL    C   +    DRR    SS ++ H    G TGL NLGNTC+MNAALQ +FNT+ LA+YF    H +E+N  N LG KG +A R+ +L+K+VW    +++AP KLR  + +Y P FAG  Q D+QELL +LLD LHEDLNRV +KPY ELKDS+GR D +VAAEAW  H  RN+SI++DLF+GQ KSKV+C  CGHESVRFDPF+ L+LPLP+E+ I+ EV+VI  DGSVPIKYG  L+ + KYS+++ +LS +C +PP  +++ E+   Q R +  +++K++  +   +Y Y+       T S S L+ + +Q   +IQR+
Sbjct:    5 ACGGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDA-GTFIIKSDYVRNVN----LAPFESV-SLFAQSERVNFEQFQDWIIKHRNATVLSKWLLSDNC-VSLTSELETPTFYQSLAGVTHLEEKDIGDLEKEFWRLKNTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQYKDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQYTQHTTHT----------------CDYCKRTASQRTAVDEALVCDE-------SFNTHSLEQHDSYSLGSGTGSASGSGSASSGISAGRHCGPVRPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNRWYGDNLPLPRQVIQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLA-------SGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVH--APGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRS 967          

HSP 2 Score: 364.77 bits (935), Expect = 1.130e-101
Identity = 231/655 (35.27%), Postives = 328/655 (50.08%), Query Frame = 0

Query: 1073 ENELSDAIVSQSERGQTSLPLTN--GPSSLESTQLRRTP----SPQS--GGGGGEAIHSRQGSSSSSGISS--------------DPPTSLEHQQQQSVSNDIADCQQGV---------------LVAMHRKMMPQEVYFLSMEKYRPVLFGIPLIVPCNETTTYQDLYKSVWTQVSRLVSPLPPRDSSTNHATDCDDSLRYEYPFNLKTVQKDGSWCSQCSWQLYCRGCPLPC------------------------------------------------YSEPYKFMSSY-LAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFLAAVPAKTVQRYRALKDGGGTVLLPGTIDEISEPSSLTSSLDLMDDDDEAFSDYTPNGTINGHSPHS--------HLSNGVDGVRGRKRMESTSLLTHTIKDDQLEDFHQHKLAEGHNPLEIDYNLYSMVCHSGVLAGGHYVSYGRHSDKKWYLQNDSACKPINEDS-MDKSTAYLLFYEREGLCKSEYLPKIEAEKSLPDTRDLD---EELETDLKKTCFI 1629
            +  +S A +  +E   +S+  TN  G +S+ES+     P     P S   G GGE    R   + SSG+S+              +   + +H Q   ++    +   GV               LVA+HRK+   + YFLS  K RP LFG+PL++P +E  T++DLY +VW QVSRL+SPLP      NHA DCDDSL Y++PF L+ V+ DG  C+ C W  +CRGC + C                                                Y+   K+   + +AIDWD TALHLRY S  ER + +  ++  S R   E + L  CL AFT EE+L  ++ Y+CS CK  + A KK Q+W+LPPIL                      IVH KRF+ VNG+W+KS K+V FP   FDPTP+LA+VP +T+ R++ L +      +    +E+       S LD +D   +   +  PN T +  +  S          S   + VR R+R+ STSL    I D + ED+HQH+L    +  +  Y LY++V HSG+L GGHY+SY  ++   WY  NDS+C+ I++   +D S AYLLFYER+GL    YLP IE  ++LP+T  +    +E E +LKK C I
Sbjct: 1092 QKRVSSAKLLHTESNTSSMSYTNHSGENSMESSLTEPIPLADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKL--EQWYHCSHCKGKKPATKKLQIWKLPPIL----------------------IVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQETILRHKELLELKNDAEMTMATNEV------VSELDEIDAPSKEVKEELPNQTGSTKATASPPPTGNILRQSKTKNAVR-RQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYISYASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEG-RTLPNTASVPLEVDETEGELKKLCSI 1714          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. SwissProt
Match: gi|50403738|sp|P35125.2|UBP6_HUMAN (RecName: Full=Ubiquitin carboxyl-terminal hydrolase 6; AltName: Full=Deubiquitinating enzyme 6; AltName: Full=Proto-oncogene TRE-2; AltName: Full=Ubiquitin thioesterase 6; AltName: Full=Ubiquitin-specific-processing protease 6)

HSP 1 Score: 356.681 bits (914), Expect = 5.559e-100
Identity = 168/319 (52.66%), Postives = 225/319 (70.53%), Query Frame = 0

Query:  722 EVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKGMNGSCIYAYEFHSTMSRSKLTHNEQQSFS 1040
            EV ++D +WPEE++   N++   R          KG TGL+NLGNTC+MN+++QCV NT+ L +YF    HLYELNRTNP+GMKGH+AK +GDL++++W    K++AP KLR TIA+Y P F G QQQD+QELL+FLLDGLHEDLNRV +KPYVELKDSDGR D  VAAEAWDNH+ RN+SI+VDLFHGQ +S+V CK CGH SVRFDPF FL+LPLPM+S + LE+ VIK DG+ P++YGL L+M+ KY+ ++ +L  +C +    ++L E+     +  P + QK++      + A+E     S    +   Q  FS
Sbjct:  499 EVHNKDMSWPEEMSFTANSSKIDRQKVPTE----KGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKSVAPLKLRRTIAKYAPKFDGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHLRRNRSIIVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMDLEITVIKLDGTTPVRYGLRLNMDEKYTGLKKQLRDLCGLNSEQILLAEVHDSNIKNFPQDNQKVQLSVSGFLCAFEIPVPSSPISASSPTQIDFS 813          

HSP 2 Score: 266.159 bits (679), Expect = 8.629e-71
Identity = 141/311 (45.34%), Postives = 182/311 (58.52%), Query Frame = 0

Query: 1159 GVLVAMHRKMMPQEVYFLSMEKYRPVLFGIPLIVPCNETTTYQDLYKSVWTQVSRLVSPLPPRDSSTNHATDCDDSLRYEYPFNLKTVQKDGSWCSQCSWQLYCRGCPLPCYSEPYKFMSSYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFLAAVPAKTVQRYRALKDGGGTVLLPGTIDEISEPSSLTSSLDLMDDDDEA 1469
            G ++A+HRKMM  E+YFLS ++ RP LFG+PLIVPC   T  +DLY +VW QVS L  PLPP+++S  HA D D+ + Y+YPF L+ VQKDG+ C+ C    +CRGC + C  +     ++Y+A+DW  TALHLRY ++QER   +  SV +S RA  E I L  CL AFT EEELGE E YYCS CK+H LA KK  LWRLPP L                      I+H KRF +VN +WIKS KIV F  + FDP+ FL        Q ++ L   G         DE+S+P  L   +  +D    A
Sbjct:  872 GYIIAVHRKMMRTELYFLSPQENRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSWLARPLPPQEASI-HAQDRDNCMGYQYPFTLRVVQKDGNSCAWCPQYRFCRGCKIDCGEDRAFIGNAYIAVDWHPTALHLRYQTSQERVVDKHESVEQSRRAQAEPINLDSCLRAFTSEEELGESEMYYCSKCKTHCLATKKLDLWRLPPFL----------------------IIHLKRFQFVNDQWIKSQKIVRFLRESFDPSAFLVPRDPALCQ-HKPLTPQG---------DELSKPRILAREVKKVDAQSSA 1149          

HSP 3 Score: 105.916 bits (263), Expect = 1.703e-21
Identity = 48/113 (42.48%), Postives = 78/113 (69.03%), Query Frame = 0

Query: 1513 DDQLEDFHQHKLAEGHNPLEIDYNLYSMVCHSGVLAGGHYVSYGRHSDKKWYLQNDSACKPINEDSMDKSTAYLLFYEREGLCKSEYLPKIEAEKSLPDTRDLDEELETDLKK 1625
            DDQ ED H          ++  YNLY++ CHSG+L+GGHY++Y ++ + KWY  NDS+C+ ++ D +D  +AY+LFYE++G+  +++LPKI+ +K + DT   DE+ E+D +K
Sbjct: 1300 DDQREDTH----------IKPIYNLYAISCHSGILSGGHYITYAKNPNCKWYCYNDSSCEELHPDEIDTDSAYILFYEQQGIDYAQFLPKIDGKK-MADTSSTDEDSESDYEK 1401          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. SwissProt
Match: gi|251757432|sp|P51784.3|UBP11_HUMAN (RecName: Full=Ubiquitin carboxyl-terminal hydrolase 11; AltName: Full=Deubiquitinating enzyme 11; AltName: Full=Ubiquitin thioesterase 11; AltName: Full=Ubiquitin-specific-processing protease 11)

HSP 1 Score: 303.523 bits (776), Expect = 1.205e-84
Identity = 184/509 (36.15%), Postives = 263/509 (51.67%), Query Frame = 0

Query:  518 QAASSNTKTRPGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYG---GSPALPRQVIRNKAGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQD-GSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKG-MNGSCIYAYE 1021
            Q    ++ T PG I+NA L Q  ++          +LK    L  G+D+ L+P   W  LV WYG   G P + R+VI     + ++E+ P+ + L++H  + +              + T+  S T+S  G  + T   R+                       L    +D RLW    E  ++ L D  +T+ DA L+    I++E R +DGTWP     + NNN               G+ GLTNLGNTC+MN+ALQC+ N   L EYF  NC+L ELN  NPLGMKG IA+ + DL+K  W   +++I P   +  +  +   F G QQ D+QELLSFLLDGLHEDLNRV  K YVEL D+ GR D  VA EAW NH  RN S++VD FHG FKS + C  CG+ SV FDPF +L++PLP+     LEV  I  D    P ++ L +  + K S++   LS+   I P  +++ ++   +F  +   E+ +   ++   I+ YE
Sbjct:  118 QGGDQDSSTFPGCINNATLFQDEIN---------WRLKEG--LVEGEDYVLLPAAAWHYLVSWYGLEHGQPPIERKVIELPNIQ-KVEVYPVELLLVRHNDLGK--------------SHTVQFSHTDS-IGLVLRTARERF-----------------------LVEPQEDTRLWAKNSEGSLDRLYDTHITVLDAALETGQLIIMETRKKDGTWPSAQLHVMNNNMSEEDEDFKGQ---PGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNCYLEELNFRNPLGMKGEIAEAYADLVKQAWSGHHRSIVPHVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPISHKRVLEVFFIPMDPRRKPEQHRLVVPKKGKISDLCVALSKHTGISPERMMVADVFSHRFYKLYQLEEPLSSILDRDDIFVYE 573          

HSP 2 Score: 78.9518 bits (193), Expect = 2.548e-13
Identity = 54/180 (30.00%), Postives = 80/180 (44.44%), Query Frame = 0

Query: 1241 FNLKTVQKDGSWCSQCSWQLYCRGCPLPCYSEPYKFMSSYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFL 1420
            F L+TV  +G+     S        P   +++PY      +AIDW+       Y   +   + +   VG  ++ A   + L++C+E FT  E L ++  +YC SCK HQLA KK  LW LP IL                      I+H KRF Y      K   +V+FPI+  D + F+
Sbjct:  717 FTLQTVNSNGTSDRTTS--------PEEVHAQPY------IAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVMKKA--PVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKKLDLWMLPEIL----------------------IIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFV 858          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. SwissProt
Match: gi|342187159|sp|Q99K46.4|UBP11_MOUSE (RecName: Full=Ubiquitin carboxyl-terminal hydrolase 11; AltName: Full=Deubiquitinating enzyme 11; AltName: Full=Ubiquitin thioesterase 11; AltName: Full=Ubiquitin-specific-processing protease 11)

HSP 1 Score: 285.419 bits (729), Expect = 5.980e-79
Identity = 180/509 (35.36%), Postives = 255/509 (50.10%), Query Frame = 0

Query:  518 QAASSNTKTRPGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYG---GSPALPRQVIRNKAGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQD-GSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKG-MNGSCIYAYE 1021
            +    +  T PG I+NA L +  +S                +L  G D+ L+P   W  +V WYG   G P + R+VI    G  ++E+ PL + L++H  +                A TI  S T+S                      +T   Q        L    +D RLW    E  ++ L +  +TL DA L+    +++E R++DGTWP       NN  D    F        G+ GLTNLGNTC+MN+ALQC+ N   L EYF  N +L ELN  NPLGMKG +A+ + DL+K  W   +++I P   +  +  +   F G QQ D+QELLSFLLDGLHEDLNRV  K YVEL +  GR DL VA EAW NH  RN S++VD FHG FKS + C  CG+ SV FDPF +L++PLP+ S   LEV  +  D    P ++ + +  +   S++   LS    + P  +I+ ++   +F  +   E  + G ++   I+ YE
Sbjct:   67 KGGDQDASTFPGCINNAGLFEDQIS-----------WHLRERLLEGDDYVLLPAPAWNYMVSWYGLMDGQPPIERKVIE-LPGIRKVEVYPLELLLVQHSDM--------------ETALTIQFSYTDS-----------------VELVLQTAREQF-------LVEPQEDTRLWTKNSEGSLDRLCNTQITLLDACLETGQLVIMETRNKDGTWPSAQLCGMNNIPDEDEDFQGQ----PGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNRYLEELNFRNPLGMKGELAEAYADLVKQTWSGYHRSIVPNVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKKEYVELCNGAGRPDLEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPVCSRRVLEVFFVPMDPRRKPEQHRVVVPKKGNISDLCVALSTHTSVAPDKMIVADVFSHRFYKLYQLEDPLSGILDRDDIFVYE 521          

HSP 2 Score: 75.8702 bits (185), Expect = 2.075e-12
Identity = 44/141 (31.21%), Postives = 66/141 (46.81%), Query Frame = 0

Query: 1280 YLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFL 1420
            Y+A+DW+       Y   +   + +   VG  L+ +   + LK+C++ FT  E L ++  +YCSSCK HQLA KK  LW LP +L                      I+H KRF +      K   +V FPI+  D + F+
Sbjct:  701 YIAMDWEPDMKRRYYDEVEAEGYVKHDCVGYMLKKS--PVQLKECIKLFTTVETLEKENPWYCSSCKQHQLATKKLDLWMLPEVL----------------------IIHLKRFSFSKISREKLDTLVQFPIRDLDFSEFV 817          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. SwissProt
Match: gi|81882675|sp|Q5D006.1|UBP11_RAT (RecName: Full=Ubiquitin carboxyl-terminal hydrolase 11; AltName: Full=Deubiquitinating enzyme 11; AltName: Full=Ubiquitin thioesterase 11; AltName: Full=Ubiquitin-specific-processing protease 11)

HSP 1 Score: 280.796 bits (717), Expect = 2.319e-77
Identity = 177/509 (34.77%), Postives = 254/509 (49.90%), Query Frame = 0

Query:  518 QAASSNTKTRPGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYG---GSPALPRQVIRNKAGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQD-GSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKG-MNGSCIYAYE 1021
            +    +  T PG I+N+ L +  +S                +L  G D+ L+P   W  LV WYG     P + R+VI    G  ++E+ P+ + L++H  +                A TI  S ++S                      +T   Q        L    +D RLW    E  ++ L +  +TL DA L+    +++E R++DGTWP       NN  D    F        G+ GLTNLGNTC+MN+ALQC+ N   L EYF  N +L ELN  NPLGMKG +A+ + DL+K  W   +++I P   +  +  +   F G QQ D+QELLSFLLDGLHEDLNRV  K YVEL +  GR DL VA EAW NH  RN S++VD FHG FKS + C  CG+ SV FDPF +L++PLP+ S   LEV  +  D    P ++ + +  +   S++   LS    + P  +I+ ++   +F  +   E  + G ++   I+ YE
Sbjct:   67 KGGDQDASTFPGSINNSGLFEDQIS-----------WHLRERLVEGDDYVLLPAPAWNYLVSWYGLKDDQPPIERKVIE-LPGIRKVEVYPIELLLVQHSDM--------------ETALTIQFSYSDS-----------------VDLVLQTAREQF-------LVEPQEDTRLWTKNSEGSLDRLCNTQITLLDACLETGQLVIMETRNKDGTWPSAQLCGMNNMPDEDEDFQGQ----PGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLNNRYLEELNFRNPLGMKGELAEAYADLVKQTWSGYHRSIVPNVFKNKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKKEYVELCNGAGRPDLEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPVCSRRVLEVFFVPMDPRRKPEQHRVVVPKKGNISDLCVALSTHTSVAPDKMIVADVFSHRFYKLYQLEDPLSGILDRDDIFVYE 521          

HSP 2 Score: 75.485 bits (184), Expect = 2.676e-12
Identity = 44/141 (31.21%), Postives = 65/141 (46.10%), Query Frame = 0

Query: 1280 YLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFL 1420
            Y+A+DW+       Y   +   + +   VG  L+     + LK+C++ FT  E L ++  +YCSSCK HQLA KK  LW LP +L                      I+H KRF +      K   +V FPI+  D + F+
Sbjct:  701 YIAMDWEPEMKRRYYDEVEAEGYVKHDCVGYMLKK--NPVQLKECIKLFTTVETLEKENPWYCSSCKQHQLATKKLDLWMLPEVL----------------------IIHLKRFSFSKFSREKLDTLVQFPIRDLDFSEFV 817          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. SwissProt
Match: gi|81872777|sp|Q9R085.1|UBP15_RAT (RecName: Full=Ubiquitin carboxyl-terminal hydrolase 15; AltName: Full=Deubiquitinating enzyme 15; AltName: Full=Ubiquitin carboxyl-terminal hydrolase of 109 kDa; AltName: Full=Ubiquitin thioesterase 15; AltName: Full=Ubiquitin-specific-processing protease 15)

HSP 1 Score: 279.641 bits (714), Expect = 8.597e-77
Identity = 178/518 (34.36%), Postives = 257/518 (49.61%), Query Frame = 0

Query:  528 PGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYG---GSPALPRQVIRN----KAGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPE-------EITSLC------NNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDG-SVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQF-RAIPMEEQKIKGMNGSCIYAYEFH 1023
            PG IDN+ L++           +G        L    D+ L+P   W  LV WY    G   + R+V+      K  +VE+ L  L         +    N+++VV                          TRR+       +    IR+I +    +      + RLW          L     T++DAGL     +++E ++ DGTWP        + ++ C        N D      NN     G+ GL+NLGNTC+MN+A+QC+ NT  L EYF  + +  ELN  NPLGM+G IAK + +LIK +W      + P   +  + R+ P F+G QQQD QELL+FLLDGLHEDLNR+  KPY++LKD+DGR D VVA EAW+NH+ RN SI+VD+FHG FKS + C  C   SV FDPF +LTLPLPM+    LEV +++ D  + P++Y + +       ++   LS +  +P   +I+ +I   +F R   M+E     M    IY +E +
Sbjct:   62 PGPIDNSGLLK-----------DGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELK--------LCENGNMNNVV--------------------------TRRFSKADTIDTIEKEIRKIFNIPDEK------EARLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSAPNVKNSNYCLPSYTAYKNYDYSEPGRNNE--QPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERSLEVYLVRMDPLAKPMQYKVIVPKIGNILDLCTALSALSGVPADKMIVTDIYNHRFHRIFAMDENLSSIMERDDIYVFEIN 526          

HSP 2 Score: 85.1149 bits (209), Expect = 3.121e-15
Identity = 53/145 (36.55%), Postives = 69/145 (47.59%), Query Frame = 0

Query: 1279 SYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATES---ITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFL 1420
            S+LA+DWD   L  RY    + +  ED    ES+         + LK C+E FT +E+LG ++ +YC +CK HQ A KK  LW LPP+LV                      VH KRF Y      K   +VDFPI   D + FL
Sbjct:  718 SFLALDWDPD-LKKRYF---DENAAEDFEKHESVEYKPPKRPFVKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLV----------------------VHLKRFSYSRYMRDKLDTLVDFPISDLDMSEFL 836          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. SwissProt
Match: gi|122135735|sp|Q2HJE4.1|UBP15_BOVIN (RecName: Full=Ubiquitin carboxyl-terminal hydrolase 15; AltName: Full=Deubiquitinating enzyme 15; AltName: Full=Ubiquitin thioesterase 15; AltName: Full=Ubiquitin-specific-processing protease 15)

HSP 1 Score: 278.87 bits (712), Expect = 1.621e-76
Identity = 178/518 (34.36%), Postives = 256/518 (49.42%), Query Frame = 0

Query:  528 PGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYG---GSPALPRQVIRN----KAGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPE-------EITSLC------NNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDG-SVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQF-RAIPMEEQKIKGMNGSCIYAYEFH 1023
            PG IDN+ L++           +G        L    D+ L+P   W  LV WY    G   + R+V+      K  +VE+ L  L         +    N+++VV                          TRR+       +    IR+I +    +      + RLW          L     T++DAGL     +++E ++ DGTWP        + ++ C        N D      NN     G+ GL+NLGNTC+MN+A QC+ NT  L EYF  + +  ELN  NPLGM+G IAK + +LIK +W      + P   +  + R+ P F+G QQQD QELL+FLLDGLHEDLNR+  KPY++LKD+DGR D VVA EAW+NH+ RN SI+VD+FHG FKS + C  C   SV FDPF +LTLPLPM+    LEV +++ D  + P++Y + +       ++   LS +  +P   +I+ +I   +F R   M+E     M    IY +E +
Sbjct:   62 PGPIDNSGLLR-----------DGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELK--------LCENGNMNNVV--------------------------TRRFSKADTIDTIEKEIRKIFNIPDEK------ETRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPNVKNSNYCLPSYTAYKNYDYSEPGRNNE--QPGLCGLSNLGNTCFMNSAFQCLSNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKYSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERTLEVYLVRMDPLTKPMQYKVVVPKIGNILDLCTALSLLSGVPADKMIVTDIYNHRFHRIFAMDENLSSIMERDDIYVFEIN 526          

HSP 2 Score: 85.1149 bits (209), Expect = 2.991e-15
Identity = 53/145 (36.55%), Postives = 69/145 (47.59%), Query Frame = 0

Query: 1279 SYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATES---ITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFL 1420
            S+LA+DWD   L  RY    + +  ED    ES+         + LK C+E FT +E+LG ++ +YC +CK HQ A KK  LW LPP+LV                      VH KRF Y      K   +VDFPI   D + FL
Sbjct:  718 SFLALDWD-PDLKKRYF---DENAAEDFEKHESVEYKPPKKPFVKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLV----------------------VHLKRFSYSRYMRDKLDTLVDFPINDLDMSEFL 836          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. SwissProt
Match: gi|426023756|sp|F6Z5C0.2|UBP15_XENTR (RecName: Full=Ubiquitin carboxyl-terminal hydrolase 15; AltName: Full=Deubiquitinating enzyme 15; AltName: Full=Ubiquitin thioesterase 15; AltName: Full=Ubiquitin-specific-processing protease 15)

HSP 1 Score: 273.863 bits (699), Expect = 1.018e-74
Identity = 183/548 (33.39%), Postives = 270/548 (49.27%), Query Frame = 0

Query:  528 PGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYGGSPALPRQVIRNKAGEVELELNPLNVKL-LKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLD-DMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPE------------------EITSLCNNNSDRRSSFLNN---------------------HLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDG-SVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQF-RAIPMEEQKIKGMNGSCIYAYEFHSTMSRSKLT 1032
            PG IDN+ L++   ++          LK +  L    D+ L+P   W  LV WY  S    +Q I  K  E  + +    V++ L    +    N+++VV                          TRR       FS+  TI  I   + T      + + RLW          L     T++DAGL     +++E ++++G WP                     +SL NN ++  S  + N                     H    G+ GL+NLGNTC+MN+A+QC+ NT  L EYF  + +  ELN  NPLGM+G IAK + +LIK +W      + P   +  + R+ P F+G QQQD QELL+FLLDGLHEDLNR+  KPY++LKD+DGR D VVA EAW+NH+ RN SI+VD+FHG FKS + C  C   SV FDPF +LTLPLPM+    LEV +++ D  + P++Y + +       ++   LS +  I P  +++ +I   +F R   M+E     M    IY +E  ++++R++ T
Sbjct:   62 PGPIDNSGLLKDTDTQ---------SLKEH--LIDELDYILLPTEGWNKLVSWY--SVVESQQPIARKVVEQGMFVKHCKVEVYLTELKLCENGNMNNVV--------------------------TRR-------FSKADTIDMIEKEMRTIFSIPDEKETRLWNKYMSNTFEPLNKPESTIQDAGLYQGQMLVIEQKNQEGQWPRGSMPKSSGIPNFSTLPKISPSSLSNNYNNFNSRIMKNSNYCLPSYAAYKNYEYSEPGRHNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQDELNMDNPLGMRGEIAKSYAELIKQMWSGKYSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHIKRNDSIIVDIFHGLFKSTLVCPECSKISVTFDPFCYLTLPLPMKKERALEVYLVRMDPLAKPMQYKVIVPKIGNIMDLCTALSSLSGIAPEKMVVTDIYNHRFHRIFAMDENLSSIMERDDIYVFE--TSINRTEDT 561          

HSP 2 Score: 84.7297 bits (208), Expect = 4.465e-15
Identity = 52/145 (35.86%), Postives = 70/145 (48.28%), Query Frame = 0

Query: 1279 SYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATES---ITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFL 1420
            SYLA+DWD   L  ++    + +  ED    ES+    +    + LK C+E FT +E+LG ++ +YC +CK HQ A KK  LW LPP+LV                      VH KRF Y      K   +VDFPI   D + FL
Sbjct:  747 SYLALDWD-PKLKKKFF---DENAAEDFEKHESVDFTPQKKAFMKLKDCIELFTTKEKLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLV----------------------VHLKRFSYSRYMRDKLDTLVDFPISDLDMSTFL 865          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. SwissProt
Match: gi|302595968|sp|B2GUZ1.1|UBP4_RAT (RecName: Full=Ubiquitin carboxyl-terminal hydrolase 4; AltName: Full=Deubiquitinating enzyme 4; AltName: Full=Ubiquitin thioesterase 4; AltName: Full=Ubiquitin-specific-processing protease 4)

HSP 1 Score: 271.166 bits (692), Expect = 5.855e-74
Identity = 184/557 (33.03%), Postives = 268/557 (48.11%), Query Frame = 0

Query:  528 PGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYG---GSPALPRQVIRN----KAGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEI----------------------------TSLCNNNSDRRSSFLNN----------------------HLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSV-PIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAI-PMEEQKIKGMNGSCIYAYEFHST 1025
            PG IDN+ L             E   LK +  L    D+ L+P   W  L+ WYG   G   + R+V+ +    K  +VE+ L  L +KL ++   + P NV S     +   ATI                                +R++ +  + R      + RLW          L     T++DAGL     +++E ++ DGTWP +                              SL  N     SS ++N                      H+   G+ GL NLGNTC+MN+ALQC+ NT  L EYF  + +  E+NR NPLGMKG IA+ + +LIK +W   +  +AP   +  + R+ P F+G QQQD+QELL+F+LDGLHEDLNRV  KPY+E KD++GR D VVA EAW+NH +RN S++VD FHG FKS + C  C   SV FDPF +LTLPLP++    +EV ++  D    PI+Y +T+ +    S++   LS++  I   N+++ ++   +F  I  M+E          I+ YE  +T
Sbjct:   66 PGPIDNSGLFSD---------PESQTLKEH--LIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYL--LELKLCEN---SDPTNVLSCHFSKADTIATIEKE-----------------------------MRKLFNIPAER------ETRLWNKYMSNTYEQLSKLDNTIQDAGLYQGQVLVIEPQNEDGTWPRQTLQSKSSTAPSRNFTTSSKPSASPYSSMSASLIANGDSTNSSGMHNSGVSRGGAGFSASYNCQEPPSPHIQ-PGLCGLGNLGNTCFMNSALQCLSNTGPLTEYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFILDGLHEDLNRVKKKPYLEPKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRIMEVFLVPADPHCRPIQYRVTVPLMGAISDLCEALSKLSGIAAENMVVTDVYNHRFHKIFQMDEGLSHITPRDDIFVYEICTT 570          

HSP 2 Score: 87.0409 bits (214), Expect = 8.937e-16
Identity = 50/149 (33.56%), Postives = 73/149 (48.99%), Query Frame = 0

Query: 1279 SYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWI-KSHKIVDFPIKGFDPTPFLAAVPAK 1426
            S LAIDWD     L +   +  + ++  S+ +  +    +I L++C+E FT  E LGE + +YC +CK HQ A KKF LW LP ILV                      VH KRF Y N  W  K   +V+FP++  + + F+    A+
Sbjct:  734 STLAIDWDSETRSLYFDEQESEACEKHTSMSQPQKKKKAAIALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPKILV----------------------VHLKRFSY-NRYWRDKLDTVVEFPVRALNMSEFVCDRAAR 859          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. nr
Match: gi|632958046|ref|XP_007894814.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 32 [Callorhinchus milii])

HSP 1 Score: 1148.65 bits (2970), Expect = 0.000e+0
Identity = 624/1441 (43.30%), Postives = 848/1441 (58.85%), Query Frame = 0

Query:    1 MGGKESKACPISSEDALKRVSETERRRLQDAFRRLASHAGSQLSGALTAHAGTLSRHVFLREVLGEGVPSALADRIFSLCGGGPVGGGGPSLASGGRGLNFREVLTALVLITRGTPEEKLKFLFGILASDSHNTHIERSHLMRFLRDCDPSASSEAVQSLFESGDKVNYETFAHWIDEHPRAFSVSEWLLVPNLHLNLVNKLDTPTFYQTLAGVTHLEEQEIVELEKRFWTMAGNSATGQIDLAAITPLVSPPLPLILVDSFFSALDENLDGHIDFKELSCGVSAACRGPELERQKFCFKIFDGDRDGVLNERELAIMCECLVLVRNQTAQGGIP---IDVNLMVESIFEKHDPKGAGVLTLQEYLVWTVDNPFPVDFSRLIFHICHIVLGLRPVSRSDERDIVLGWLEKEERAPLTVGSIWHLINMDWWNHWQAYVSHTPGSPTLPPSSVQSSPSELVTSSGKRAGKRVPTLDSTLAMDSNSGVVGTSYQPLNESGITSGKTVATTTPS------ASPHPSRRSSQ---AASSNTKT-----RPGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYGGSPALPRQVIRN-KAGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHL--NVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKGMNGSCIYAYEFHSTMSRSKLTHNEQQSFSEIQRNVQSPTKMLVNGVPKMNGMGPPKTDEKAENELSDAIVSQSERGQTSLPLTNGPSSLESTQLRRTPSPQSGGGGGEAIHSRQGSSSSSGISSDPPTSLEHQQQQSVSNDIADCQQGVLVAMHRKMMPQEVYFLSMEKYRPVLFGIPLIVPCNETTTYQDLYKSVWTQVSRLVSPLPPRDSSTNHATDCDDSLRYEYPFNLKTVQKDGSWCSQCSWQLYCRGCPLPCYSEPYKFMSSYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFLA 1421
            MG KES+   +S ++A KRV++ E +RL+DAF+R         +  L+ +   +++  F++EVLG+GVP  +A+ I+   GG              +GL+F  ++  LVL+TRG  EEK K++F + A+++  +++ R  +   LR CD       ++  F  G+KVNYE F +W+ ++  AF+ + WLL   + + L +  DTPTFYQTLAGVTHLEE +I++LEKR+W +   S TG+ DL    PLVSPP+ L L +  FSA DEN D HIDFKE+SCG+SA CRGP  ERQKFCFK+FD DRDG L+  EL  M   L+ V        IP   +    +V  I +KHD    G LTL++Y +W+V +    +F  L+F +CHIVLGLRP +  +E  I+ GWLE+E R  L  G  W LI++ WW  W+ YV +      + PSS+ ++             +  P L +         +   S    +E+  ++G +++  +P+      A  H S  ++Q    A+ N+ +     +PG IDN CLV    ++  +LT EGG+LK +  L RGKD+E++PE +WRAL  WYG + +LPR VI+N ++   ELEL P  +  L+ Q  TR    +  V               N G   +  T  +R  AY   FSR  TI+ I ++LS RL  K +DMRLW +  E  + LL+DE  TLED  + DE  +++EVR++D +WPEE++ + N      SS ++ H     KG TGL+NLGNTC+MN+++QCV NT+ L +YF    HLYELNR NPLGMKGH+AK +GDLI+++W    K IAP KLR TIA+Y P F G QQQD+QELL+FLLDGLHEDLNRV DKPYVELKDSDGR D  VAAEAWDNH+ RNKSIVVDLFHGQ KS+V CK+CGH S RFDPF FL+LPLPM+S +HLE+ VIK DGS P++YGL L+M+ KY+ ++ +LS +C +    ++L E+     +  P + QK++  +   + A+E     S    T               SP +   + VP +NG           N+ S+A  ++       +P T  P+  E       P+    GGG                  D P++                  G ++A+HRKMM  E+YFLS +K RP LFG+PLIVPC   T  +DLY +VWTQVSRL SPLPP+++S NHA DCDDS+ Y+YPF L+ V KDG+ C+ C W  +CRGC + C  E      +Y+AIDWD TALHLRY ++QER  +E  SV +S RA  E I L  CL AFT EEELG DE YYCS CK+ +LA KK  LWRLPPIL                      I+H KRF +VNGRWIKS KIV FP +GFDP+ FL 
Sbjct:    1 MGAKESRIGFLSYDEAAKRVTDVELKRLKDAFKR---------TSGLSIY---MTQQCFVKEVLGDGVPLKVAEVIYQSFGGT------------SKGLHFNNLIVGLVLLTRGRDEEKAKYIFNLFANEA-GSYVVREEMETMLRTCDGEVPL-GLKKCFAEGEKVNYEKFKNWLLQNKDAFTFARWLLSGGVCVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFIPLVSPPIHLSLSEGLFSAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDTDRDGALSRTELEEMVVALLEVWKDNRTDKIPELSMATPDIVNYILKKHDTTKLGQLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIISGWLERESRHGLQPGHNWFLISVQWWKQWKEYVKYETKPVVIEPSSIMNAVRSQTLLHSMDQVEAKPGLQNFFTAADEKYLDNISIA--SEATESTGTSLSPLSPTSTEVCFARQHNSSDNNQCFLGANGNSFSQLGPQKPGAIDNQCLVMQESAKAPTLTLEGGRLKRSPNLVRGKDYEIVPEPVWRALYHWYGANLSLPRPVIKNTQSNLAELELFPRYLLFLRQQPATRTQQSNIWV---------------NMGNVPSPTTPLKRVLAYTGCFSRVQTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEEHTLEDLKIPDEQHLVIEVRNKDMSWPEEMSFIAN------SSKMDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTKYFISGRHLYELNRANPLGMKGHMAKCYGDLIQELWSGAQKNIAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHDKPYVELKDSDGRPDYEVAAEAWDNHLRRNKSIVVDLFHGQLKSQVKCKMCGHISARFDPFNFLSLPLPMDSYMHLEITVIKLDGSTPVRYGLRLNMDEKYTGLKKQLSELCGLKAEQILLGEVQISNIKNFPQDNQKVRLSSNGFLCAFEVPVPGSPCSAT---------------SPVETDSSPVPFVNG---------RHNQSSNANPTKPNLIPNGIPSTVVPAGTEKNLCNGFPNGYLPGGG------------------DSPST------------------GYIIAVHRKMMRTELYFLSAQKIRPSLFGMPLIVPCTVHTRKKDLYDAVWTQVSRLASPLPPQEAS-NHAQDCDDSMGYQYPFTLRVVNKDGNTCAWCPWYRFCRGCKVDCNEERTAVGPAYIAIDWDPTALHLRYQTSQERICEEHESVEQSRRAQAEPINLDSCLRAFTNEEELGGDELYYCSKCKTLRLATKKLDLWRLPPIL----------------------IIHLKRFQFVNGRWIKSQKIVKFPREGFDPSAFLV 1309          

HSP 2 Score: 114.005 bits (284), Expect = 2.187e-21
Identity = 46/95 (48.42%), Postives = 70/95 (73.68%), Query Frame = 0

Query: 1535 YNLYSMVCHSGVLAGGHYVSYGRHSDKKWYLQNDSACKPINEDSMDKSTAYLLFYEREGLCKSEYLPKIEAEKSLPDTRDLDEELETDLKKTCFI 1629
            YNLY++ CHSG+L GGHYV+Y ++ + KWY  NDS+CK +  D +D  +AY+LFYE++G+  S++LPK E +K + DT  +DE+ E+D +K C +
Sbjct: 1497 YNLYAISCHSGILGGGHYVTYAKNPNDKWYCYNDSSCKEVRPDEIDTDSAYILFYEQQGVDYSQFLPKTEGKK-MADTSSMDEDFESDYRKYCIL 1590          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. nr
Match: gi|1042300245|ref|XP_017346444.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 32 isoform X1 [Ictalurus punctatus])

HSP 1 Score: 1144.8 bits (2960), Expect = 0.000e+0
Identity = 627/1465 (42.80%), Postives = 855/1465 (58.36%), Query Frame = 0

Query:    1 MGGKESKACPISSEDALKRVSETERRRLQDAFRRLASHAGSQLSGALTAHAGTLSRHVFLREVLGEGVPSALADRIFSLCGGGPVGGGGPSLASGGRGLNFREVLTALVLITRGTPEEKLKFLFGILASDSHNTHIERSHLMRFLRDCDPSASSEAVQSLFESGDKVNYETFAHWIDEHPRAFSVSEWLLVPNLHLNLVNKLDTPTFYQTLAGVTHLEEQEIVELEKRFWTMAGNSATGQIDLAAITPLVSPPLPLILVDSFFSALDENLDGHIDFKELSCGVSAACRGPELERQKFCFKIFDGDRDGVLNERELAIMCECLVLVRNQTAQGGIP---IDVNLMVESIFEKHDPKGAGVLTLQEYLVWTVDNPFPVDFSRLIFHICHIVLGLRPVSRSDERDIVLGWLEKEERAPLTVGSIWHLINMDWWNHWQAYVSHTPGSPTLPPSSVQSSPSELVTSSGKRAGKRVPTLDSTLAMDSNSGVVGTSYQPLNESGITSGKTVATTTPSAS-PHPSR--------RSSQAASSNTKT---------------RPGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYGGSPALPRQVIRNK-AGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKGMNGSCIYAYEFHSTMSRSKLTHNEQQSFSEIQRNVQSPTKMLVNGVPKMNGMGPPKTDEKAENELSDAIVSQSERGQTSLPLTNGPSSLESTQLRRTPSPQSGGGGGEAIHSRQGSSSSSGISSDPPTSLEHQQQQSVSNDIADCQQ---------GVLVAMHRKMMPQEVYFLSMEKYRPVLFGIPLIVPCNETTTYQDLYKSVWTQVSRLVSPLPPRDSSTNHATDCDDSLRYEYPFNLKTVQKDGSWCSQCSWQLYCRGCPLPCYSEPYKFMSSYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFLAAVPAKTV 1428
            MG KES+   +S ++A++RV++ E +RL+DAF+R         +  L+A+   +++  F +EVLG+GVP  +A+ I++  GG P            +GL+F  ++  LVL+TRG  EEK K++F + ASDS  +++ R  +   L   D      +++  F  G+KVNY+ F +W+ ++  AF+ S WLL   + + L +  DTPTFYQTLAGVTHLEE +I++LEKR+W +   S TG+ DL    PLVSPP+   L +  F A DEN D HIDFKE+SCG+SA CRGP  ERQKFCFK+FD DRDGVL+  E+  M   L+ V        +P     V+ +VE I + HD    G LTL++Y +W+V +    +F  L+F +CHIVLGLRP +  +E  I+ GWLE+E R  L  G  W LI+M WW  W+ YV +   S       V   P  L   SG+R  +  P+ +  L   S +G V  S     E    S   V+ +TP++  PH S         R      +N +                RPG IDN  LV     +  +LT EGG+LK  + L  G+DFE +PE +WRAL  WYG + +LPR VI++    + ELEL P  +  L+ Q  TR    S++      ++  +       G   +     +R  AY   FSR +TI+ I ++LS RL  K +DMRLW +  E  + LL+DE  TLE   ++DE  +++EVR++D +WPEE++ + N++   R          KG TGL+NLGNTC+MN+++QCV NTK L +YF    HLYELNR NP+GM+GH+AK +GDL++++W    K +AP KLR TIA+Y P F G QQQD+QELL+FLLDGLHEDLNRV +KPYVELKDSDGR D  VA EAWDNH+ RN+SIVVDLFHGQ KS+V CK CGH S RFDPF FL+LPLPM+SS+HLE+ VIK DGS P++YGL L+M+ KY+ ++ +LS +C + P  ++L E+     +  P + QK++      + A+E     S + +T               +P    + G    NG+               A  S SE+                      P P   G  G  I S    S ++G+   P T +    ++S++N I +            G ++AMHRKMM  E+YFLS +K RP LFG+PLIVPC   T+ +DLY +VW QVSRL SPLPP+++S NHA DCDDSL Y+YPF L+ VQKDG+ C+ C W  +CRGC + C  +     ++Y+A+DWD TALHLRY ++QER  +E  SV  S RA  E I+L  CL+AFT EEELGEDE YYCS CK+H+LA KK  LWRLPPIL                      IVH KRF +VNGRWIKS KIV FP + F+P  FLA   A++ 
Sbjct:    1 MGAKESRIGFLSYDEAVRRVTDVELKRLKDAFKR---------TSGLSAY---MTQQCFYKEVLGDGVPPKVAEVIYNSFGGTP------------KGLHFNNLIVGLVLLTRGRDEEKAKYIFSLFASDS-GSYVVREEVESRLHAVDGEVPP-SLRKCFLEGEKVNYDKFKNWLLQNKEAFTFSRWLLSGGVCVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEMHKMVVALLEVWKDNRTDTLPELESSVSDIVEEILKIHDTTKQGHLTLEDYQIWSVKSALASEFLNLLFQVCHIVLGLRPATPKEEGQIIRGWLERESRHGLQPGQNWFLISMQWWKLWKDYVKYDNKS------IVVEQPGML--GSGRRTPQSTPSAEPAL---SKTGTVARSLSSPGEKSPKSNWEVSESTPNSQLPHVSSSATDICFARQHNVLDNNNQCFSTANGHLPNHLAAQRPGAIDNQPLVTTEPIKAPTLTMEGGRLKRPLNLMAGRDFETVPEPVWRALYHWYGANLSLPRPVIKSVITDQPELELFPRYLLFLRQQPATRTPQ-SNIWVNMGMSSLRMFPPHFPRGNMPSPNAPLKRMLAYTGCFSRMSTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIEDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTE----KGATGLSNLGNTCFMNSSIQCVSNTKPLTDYFITGRHLYELNRLNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDAEVATEAWDNHLRRNRSIVVDLFHGQLKSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLNMDEKYTGLKKQLSELCSLKPEQILLAEVHSSNIKNFPQDNQKVRLSVNGFLCAFEIPVPGSPTSITS-------------PAPVANTLMG----NGI---------------ARNSHSEK----------------------PGPVPNGTAGTPIQSGSDRSLANGM---PSTVMPSGSEKSLANGIPNGHATPVQDSPFTGYIIAMHRKMMRSELYFLSSQKNRPSLFGMPLIVPCTVHTSKKDLYDAVWIQVSRLASPLPPQEAS-NHAQDCDDSLGYQYPFTLRAVQKDGNSCAWCPWYRFCRGCKVECNEDRASLGNAYIAVDWDPTALHLRYQTSQERIVEEHTSVELSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPIL----------------------IVHLKRFQFVNGRWIKSQKIVKFPCESFNPGAFLAPREAQST 1343          

HSP 2 Score: 110.153 bits (274), Expect = 3.048e-20
Identity = 45/99 (45.45%), Postives = 72/99 (72.73%), Query Frame = 0

Query: 1531 LEIDYNLYSMVCHSGVLAGGHYVSYGRHSDKKWYLQNDSACKPINEDSMDKSTAYLLFYEREGLCKSEYLPKIEAEKSLPDTRDLDEELETDLKKTCFI 1629
            LE  YNLY++ CHSG++ GGHYV+Y ++ + KWY  NDS+CK ++ + +D  +AY+LFYE++G+  S++LP  + +K + DT  +DE+ E+D KK C +
Sbjct: 1519 LEPVYNLYAISCHSGIMGGGHYVTYAKNPNDKWYCYNDSSCKEVHSEEIDTDSAYILFYEQQGVDYSQFLPVTDGKK-MADTTSMDEDFESDYKKYCVL 1616          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. nr
Match: gi|1042300249|ref|XP_017346446.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 32 isoform X3 [Ictalurus punctatus])

HSP 1 Score: 1143.64 bits (2957), Expect = 0.000e+0
Identity = 628/1465 (42.87%), Postives = 852/1465 (58.16%), Query Frame = 0

Query:    1 MGGKESKACPISSEDALKRVSETERRRLQDAFRRLASHAGSQLSGALTAHAGTLSRHVFLREVLGEGVPSALADRIFSLCGGGPVGGGGPSLASGGRGLNFREVLTALVLITRGTPEEKLKFLFGILASDSHNTHIERSHLMRFLRDCDPSASSEAVQSLFESGDKVNYETFAHWIDEHPRAFSVSEWLLVPNLHLNLVNKLDTPTFYQTLAGVTHLEEQEIVELEKRFWTMAGNSATGQIDLAAITPLVSPPLPLILVDSFFSALDENLDGHIDFKELSCGVSAACRGPELERQKFCFKIFDGDRDGVLNERELAIMCECLVLVRNQTAQGGIP---IDVNLMVESIFEKHDPKGAGVLTLQEYLVWTVDNPFPVDFSRLIFHICHIVLGLRPVSRSDERDIVLGWLEKEERAPLTVGSIWHLINMDWWNHWQAYVSHTPGSPTLPPSSVQSSPSELVTSSGKRAGKRVPTLDSTLAMDSNSGVVGTSYQPLNESGITSGKTVATTTPSAS-PHPSR--------RSSQAASSNTKT---------------RPGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYGGSPALPRQVIRNK-AGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKGMNGSCIYAYEFHSTMSRSKLTHNEQQSFSEIQRNVQSPTKMLVNGVPKMNGMGPPKTDEKAENELSDAIVSQSERGQTSLPLTNGPSSLESTQLRRTPSPQSGGGGGEAIHSRQGSSSSSGISSDPPTSLEHQQQQSVSNDIADCQQ---------GVLVAMHRKMMPQEVYFLSMEKYRPVLFGIPLIVPCNETTTYQDLYKSVWTQVSRLVSPLPPRDSSTNHATDCDDSLRYEYPFNLKTVQKDGSWCSQCSWQLYCRGCPLPCYSEPYKFMSSYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFLAAVPAKTV 1428
            MG KES+   +S ++A++RV++ E +RL+DAF+R         +  L+A+   +++  F +EVLG+GVP  +A+ I++  GG P            +GL+F  ++  LVL+TRG  EEK K++F + ASDS  +++ R  +   L   D      +++  F  G+KVNY+ F +W+ ++  AF+ S WLL   + + L +  DTPTFYQTLAGVTHLEE +I++LEKR+W +   S TG+ DL    PLVSPP+   L +  F A DEN D HIDFKE+SCG+SA CRGP  ERQKFCFK+FD DRDGVL+  E+  M   L+ V        +P     V+ +VE I + HD    G LTL++Y +W+V +    +F  L+F +CHIVLGLRP +  +E  I+ GWLE+E R  L  G  W LI+M WW  W+ YV +   S       V   P  L   SG+R  +  P+ +  L   S +G V  S     E    S   V+ +TP++  PH S         R      +N +                RPG IDN  LV     +  +LT EGG+LK  + L  G+DFE +PE +WRAL  WYG + +LPR VI++    + ELEL P  +  L+ Q  TR    +  V               N G   +     +R  AY   FSR +TI+ I ++LS RL  K +DMRLW +  E  + LL+DE  TLE   ++DE  +++EVR++D +WPEE++ + N++   R          KG TGL+NLGNTC+MN+++QCV NTK L +YF    HLYELNR NP+GM+GH+AK +GDL++++W    K +AP KLR TIA+Y P F G QQQD+QELL+FLLDGLHEDLNRV +KPYVELKDSDGR D  VA EAWDNH+ RN+SIVVDLFHGQ KS+V CK CGH S RFDPF FL+LPLPM+SS+HLE+ VIK DGS P++YGL L+M+ KY+ ++ +LS +C + P  ++L E+     +  P + QK++      + A+E     S + +T               +P    + G    NG+               A  S SE+                      P P   G  G  I S    S ++G+   P T +    ++S++N I +            G ++AMHRKMM  E+YFLS +K RP LFG+PLIVPC   T+ +DLY +VW QVSRL SPLPP+++S NHA DCDDSL Y+YPF L+ VQKDG+ C+ C W  +CRGC + C  +     ++Y+A+DWD TALHLRY ++QER  +E  SV  S RA  E I+L  CL+AFT EEELGEDE YYCS CK+H+LA KK  LWRLPPIL                      IVH KRF +VNGRWIKS KIV FP + F+P  FLA   A++ 
Sbjct:    1 MGAKESRIGFLSYDEAVRRVTDVELKRLKDAFKR---------TSGLSAY---MTQQCFYKEVLGDGVPPKVAEVIYNSFGGTP------------KGLHFNNLIVGLVLLTRGRDEEKAKYIFSLFASDS-GSYVVREEVESRLHAVDGEVPP-SLRKCFLEGEKVNYDKFKNWLLQNKEAFTFSRWLLSGGVCVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEMHKMVVALLEVWKDNRTDTLPELESSVSDIVEEILKIHDTTKQGHLTLEDYQIWSVKSALASEFLNLLFQVCHIVLGLRPATPKEEGQIIRGWLERESRHGLQPGQNWFLISMQWWKLWKDYVKYDNKS------IVVEQPGML--GSGRRTPQSTPSAEPAL---SKTGTVARSLSSPGEKSPKSNWEVSESTPNSQLPHVSSSATDICFARQHNVLDNNNQCFSTANGHLPNHLAAQRPGAIDNQPLVTTEPIKAPTLTMEGGRLKRPLNLMAGRDFETVPEPVWRALYHWYGANLSLPRPVIKSVITDQPELELFPRYLLFLRQQPATRTPQSNIWV---------------NMGNMPSPNAPLKRMLAYTGCFSRMSTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIEDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTE----KGATGLSNLGNTCFMNSSIQCVSNTKPLTDYFITGRHLYELNRLNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDAEVATEAWDNHLRRNRSIVVDLFHGQLKSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLNMDEKYTGLKKQLSELCSLKPEQILLAEVHSSNIKNFPQDNQKVRLSVNGFLCAFEIPVPGSPTSITS-------------PAPVANTLMG----NGI---------------ARNSHSEK----------------------PGPVPNGTAGTPIQSGSDRSLANGM---PSTVMPSGSEKSLANGIPNGHATPVQDSPFTGYIIAMHRKMMRSELYFLSSQKNRPSLFGMPLIVPCTVHTSKKDLYDAVWIQVSRLASPLPPQEAS-NHAQDCDDSLGYQYPFTLRAVQKDGNSCAWCPWYRFCRGCKVECNEDRASLGNAYIAVDWDPTALHLRYQTSQERIVEEHTSVELSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPIL----------------------IVHLKRFQFVNGRWIKSQKIVKFPCESFNPGAFLAPREAQST 1329          

HSP 2 Score: 110.153 bits (274), Expect = 3.037e-20
Identity = 45/99 (45.45%), Postives = 72/99 (72.73%), Query Frame = 0

Query: 1531 LEIDYNLYSMVCHSGVLAGGHYVSYGRHSDKKWYLQNDSACKPINEDSMDKSTAYLLFYEREGLCKSEYLPKIEAEKSLPDTRDLDEELETDLKKTCFI 1629
            LE  YNLY++ CHSG++ GGHYV+Y ++ + KWY  NDS+CK ++ + +D  +AY+LFYE++G+  S++LP  + +K + DT  +DE+ E+D KK C +
Sbjct: 1505 LEPVYNLYAISCHSGIMGGGHYVTYAKNPNDKWYCYNDSSCKEVHSEEIDTDSAYILFYEQQGVDYSQFLPVTDGKK-MADTTSMDEDFESDYKKYCVL 1602          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. nr
Match: gi|1049224341|ref|XP_017550335.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 32-like isoform X1 [Pygocentrus nattereri])

HSP 1 Score: 1142.49 bits (2954), Expect = 0.000e+0
Identity = 624/1472 (42.39%), Postives = 855/1472 (58.08%), Query Frame = 0

Query:    1 MGGKESKACPISSEDALKRVSETERRRLQDAFRRLASHAGSQLSGALTAHAGTLSRHVFLREVLGEGVPSALADRIFSLCGGGPVGGGGPSLASGGRGLNFREVLTALVLITRGTPEEKLKFLFGILASDSHNTHIERSHLMRFLRDCDPSASSEAVQSLFESGDKVNYETFAHWIDEHPRAFSVSEWLLVPNLHLNLVNKLDTPTFYQTLAGVTHLEEQEIVELEKRFWTMAGNSATGQIDLAAITPLVSPPLPLILVDSFFSALDENLDGHIDFKELSCGVSAACRGPELERQKFCFKIFDGDRDGVLNERELAIMCECLVLVRNQTAQGGIP---IDVNLMVESIFEKHDPKGAGVLTLQEYLVWTVDNPFPVDFSRLIFHICHIVLGLRPVSRSDERDIVLGWLEKEERAPLTVGSIWHLINMDWWNHWQAYVSHTPGSPTLPPSSVQSSPSELVTSSGKRAGKRVPTLDSTLAMDSNSGVVGTSYQ-PLNESGITSGKTVATTTPSASPHPSR--------RSSQAASSNTKT---------------RPGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYGGSPALPRQVIRNKAGE-VELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKGMNGSCIYAYEFHSTMSRSKLTHNEQQSFSEIQRNVQSPTKMLVNGVPKMNGMGPPKTDEKAENELSDAIVSQSERGQTSLPLTNGPSSLESTQLRRTPSPQSGGGGGEAIHSRQGSSSSSGISSDPPTSLEHQQQQSVSNDIADCQ---------QGVLVAMHRKMMPQEVYFLSMEKYRPVLFGIPLIVPCNETTTYQDLYKSVWTQVSRLVSPLPPRDSSTNHATDCDDSLRYEYPFNLKTVQKDGSWCSQCSWQLYCRGCPLPCYSEPYKFMSSYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFLAAVPAKTVQRYRALK 1435
            MG KES+   +S ++A+KRV++ E +RL+DAF+R         +  L+A+   +++  F REVLG+GVP  +A+ I++  GG P            +GL+F  ++  LVL+TRG  EEK K++F + A+DS  +++ R  +   LR  D      +++  F  G+KVNYE F  W+ ++  AF+ S WLL   + + L +  DTPTFYQTLAGVTHLEE +I++LEKR+W +   S TG+ DL    PLVSPP+   L +  F A DEN D HIDFKE+SCG+SA CRGP  ERQKFCFK+FD DRDGVL+  E+  M   L+ V        +P    +V+ +VE I + HD    G LTL++Y +W+V +    +F  L+F +CHIVLGLRP +  +E  I+ GWLE+E R  L  G  W LI+M WW  W+ YV +   S  +    +  +        GKR+ +     D  +A    +G V  S   P ++S   + +    TT S  PH S         R      +N +                RPG IDN  LV  +  +  +LT EGG+LK ++ L  G+DFE +PE +WRAL  WYG + +LPR VI++   +  ELEL P  +  L+ Q  TR    S++       +  +       G   +     +R  AY   FS  +TI+ I ++LS RL  K +DMRLW +  E  + LL+DE  TLE   ++DE  +++EVR++D +WPEE++ + N++   R          KG TGL+NLGNTC+MN+++QCV NTK L +YF    HLYELNRTNP+GM+GH+AK +GDL++++W    K +AP KLR TIA+Y P F G QQQD+QELL+FLLDGLHEDLNRV +KPYVELKDSDGR D  VA EAW+NH+ RN+SIVVDLFHGQ KS+V CK CGH S RFDPF FL+LPLPM+SS+HLE+ VIK DGS P++YGL L+M+ KY+ ++ +LS +C + P  ++L E+     +  P + QK++      + A+E     S + +T               + T ++ NG                                     T G S  E       P     G  G  +HS    + ++G+   P T +     +S++N I +            G ++AMHRKMM  E+YFLS +K RP LFG+PLIVPC   T+ +DLY +VW QVSRL SPLPP+++S NHA DCDDS+ Y+YPF L+ VQKDG  C+ C W  +CRGC + C  +     ++Y+A+DWD TALHLRY ++QER  +E  SV +S RA  E I+L  CL+AFT EEELGEDE YYCS CK+H+LA KK  LWRLPP+L                      IVH KRF +VNGRWIKS KIV FP  GFDP  FLA  P +  Q   +L+
Sbjct:    1 MGAKESRIGFLSYDEAVKRVTDVELKRLKDAFKR---------TSGLSAY---MTQQCFFREVLGDGVPPKVAEVIYNSFGGTP------------KGLHFNNLIVGLVLLTRGRDEEKAKYIFSLFANDS-GSYVVREEVENMLRLVDGEVPP-SLKKCFLEGEKVNYEKFKSWLLQNKEAFTFSRWLLSGGVCVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRHEVHEMVVALLEVWKDNRTDTLPELHSNVSDIVEDILKMHDTTKQGHLTLEDYQIWSVKSALANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRHGLQSGQNWFLISMQWWQLWKDYVKYNNKSIVVEQPGMHGN--------GKRSPQSTRAADPAMA---KAGAVARSLSSPGDKSPDRTWEASENTTTSQMPHVSSSATDICFARQHNVLDNNNQCFSSVNGHLPNHLAAQRPGAIDNQPLVTTDPMKAPTLTMEGGRLKRSLHLMAGRDFETVPEPVWRALYHWYGANLSLPRPVIKSVVTDHAELELFPRYLLFLRQQPATRTPQ-SNIWVNMGMTSLRMFPPHFPRGNVPSPNAPLKRVLAYTGCFSCMSTIKDIHEYLSQRLRIKEEDMRLWLYNSENDLTLLDDEDHTLESLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTE----KGATGLSNLGNTCFMNSSIQCVSNTKPLTDYFLSGRHLYELNRTNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDAEVATEAWENHLRRNRSIVVDLFHGQLKSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLNMDEKYTGLKKQLSELCSLKPEQILLAEVHSSNIKNFPQDNQKVRLSVNGFLCAFEIPVPGSPTSVT-----------SLAPTDTTLMANG-------------------------------------TAGNSHAEK------PGLIPNGTAGTPMHSGSEKNLANGM---PSTVVPSVPDRSLTNGIPNGHITLVQDSPFIGYIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTSKKDLYDAVWIQVSRLASPLPPQEAS-NHAQDCDDSMGYQYPFTLRVVQKDGHSCAWCPWYRFCRGCTVECNEDRAALGNAYIAVDWDPTALHLRYQTSQERIVEEHASVEQSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPVL----------------------IVHLKRFQFVNGRWIKSQKIVKFPRMGFDPNAFLA--PREAAQSTWSLQ 1348          

HSP 2 Score: 113.235 bits (282), Expect = 3.120e-21
Identity = 46/99 (46.46%), Postives = 74/99 (74.75%), Query Frame = 0

Query: 1531 LEIDYNLYSMVCHSGVLAGGHYVSYGRHSDKKWYLQNDSACKPINEDSMDKSTAYLLFYEREGLCKSEYLPKIEAEKSLPDTRDLDEELETDLKKTCFI 1629
            LE  YNLY++ CHSG++ GGHYV+Y ++ ++KWY  NDS+CK ++ + +D  +AY+LFYE++G+  S++LPK + +K + DT  +DE+ E+D KK C +
Sbjct: 1524 LEPLYNLYAISCHSGIMGGGHYVTYAKNPNEKWYCYNDSSCKEVHSEEVDADSAYILFYEQQGVNYSQFLPKTDGKK-MADTTSMDEDFESDYKKYCVL 1621          

BLAST of ubiquitin carboxyl-terminal hydrolase 32 vs. nr
Match: gi|1042300247|ref|XP_017346445.1| (PREDICTED: ubiquitin carboxyl-terminal hydrolase 32 isoform X2 [Ictalurus punctatus])

HSP 1 Score: 1140.95 bits (2950), Expect = 0.000e+0
Identity = 627/1465 (42.80%), Postives = 855/1465 (58.36%), Query Frame = 0

Query:    1 MGGKESKACPISSEDALKRVSETERRRLQDAFRRLASHAGSQLSGALTAHAGTLSRHVFLREVLGEGVPSALADRIFSLCGGGPVGGGGPSLASGGRGLNFREVLTALVLITRGTPEEKLKFLFGILASDSHNTHIERSHLMRFLRDCDPSASSEAVQSLFESGDKVNYETFAHWIDEHPRAFSVSEWLLVPNLHLNLVNKLDTPTFYQTLAGVTHLEEQEIVELEKRFWTMAGNSATGQIDLAAITPLVSPPLPLILVDSFFSALDENLDGHIDFKELSCGVSAACRGPELERQKFCFKIFDGDRDGVLNERELAIMCECLVLVRNQTAQGGIP---IDVNLMVESIFEKHDPKGAGVLTLQEYLVWTVDNPFPVDFSRLIFHICHIVLGLRPVSRSDERDIVLGWLEKEERAPLTVGSIWHLINMDWWNHWQAYVSHTPGSPTLPPSSVQSSPSELVTSSGKRAGKRVPTLDSTLAMDSNSGVVGTSYQPLNESGITSGKTVATTTPSAS-PHPSR--------RSSQAASSNTKT---------------RPGMIDNACLVQGNVSRVTSLTAEGGKLKTNVKLARGKDFELIPERLWRALVLWYGGSPALPRQVIRNK-AGEVELELNPLNVKLLKHQVITRPANVSSVVAGYSAAAATISASSTNSGYGSTMPTTTRRYHAYQAAFSRRTTIRQIADFLSTRLHTKLDDMRLWQFKDETQMNLLEDEALTLEDAGLKDEGSILVEVRSRDGTWPEEITSLCNNNSDRRSSFLNNHLNVKGVTGLTNLGNTCYMNAALQCVFNTKILAEYFSLNCHLYELNRTNPLGMKGHIAKRFGDLIKDVWGVDNKAIAPFKLRGTIARYQPHFAGAQQQDAQELLSFLLDGLHEDLNRVTDKPYVELKDSDGRADLVVAAEAWDNHVIRNKSIVVDLFHGQFKSKVTCKVCGHESVRFDPFTFLTLPLPMESSIHLEVIVIKQDGSVPIKYGLTLDMESKYSNIRPRLSRICKIPPANLILVEIVQCQFRAIPMEEQKIKGMNGSCIYAYEFHSTMSRSKLTHNEQQSFSEIQRNVQSPTKMLVNGVPKMNGMGPPKTDEKAENELSDAIVSQSERGQTSLPLTNGPSSLESTQLRRTPSPQSGGGGGEAIHSRQGSSSSSGISSDPPTSLEHQQQQSVSNDIADCQQ---------GVLVAMHRKMMPQEVYFLSMEKYRPVLFGIPLIVPCNETTTYQDLYKSVWTQVSRLVSPLPPRDSSTNHATDCDDSLRYEYPFNLKTVQKDGSWCSQCSWQLYCRGCPLPCYSEPYKFMSSYLAIDWDQTALHLRYLSAQERSFKEDLSVGESLRAATESITLKKCLEAFTREEELGEDEKYYCSSCKSHQLAIKKFQLWRLPPILVSLLPPAIPLKCLKLICIVILQIVHFKRFHYVNGRWIKSHKIVDFPIKGFDPTPFLAAVPAKTV 1428
            MG KES+   +S ++A++RV++ E +RL+DAF+R         +  L+A+   +++  F +EVLG+GVP  +A+ I++  GG P            +GL+F  ++  LVL+TRG  EEK K++F + ASDS  +++ R  +   L   D      +++  F  G+KVNY+ F +W+ ++  AF+ S WLL   + + L +  DTPTFYQTLAGVTHLEE +I++LEKR+W +   S TG+ DL    PLVSPP+   L +  F A DEN D HIDFKE+SCG+SA CRGP  ERQKFCFK+FD DRDGVL+  E+  M   L+ V        +P     V+ +VE I + HD    G LTL++Y +W+V +    +F  L+F +CHIVLGLRP +  +E  I+ GWLE+E R  L  G  W LI+M WW  W+ YV +   S       V   P  L   SG+R  +  P+ +  L   S +G V  S     E    S   V+ +TP++  PH S         R      +N +                RPG IDN  LV     +  +LT EGG+LK  + L  G+DFE +PE +WRAL  WYG + +LPR VI++    + ELEL P  +  L+ Q  TR    S++      ++  +       G   +     +R  AY   FSR +TI+ I ++LS RL  K +DMRLW +  E  + LL+DE  TLE   ++DE  +++EVR++D +WPEE++ + N++   R          KG TGL+NLGNTC+MN+++QCV NTK L +YF    HLYELNR NP+GM+GH+AK +GDL++++W    K +AP KLR TIA+Y P F G QQQD+QELL+FLLDGLHEDLNRV +KPYVELKDSDGR D  VA EAWDNH+ RN+SIVVDLFHGQ KS+V CK CGH S RFDPF FL+LPLPM+SS+HLE+ VIK DGS P++YGL L+M+ KY+ ++ +LS +C + P  ++L E+     +  P + QK++      + A+E     S + +T               +P    + G    NG+               A  S SE+                      P P   G  G  I S    S ++G+   P T +    ++S++N I +            G ++AMHRKMM  E+YFLS +K RP LFG+PLIVPC   T+ +DLY +VW QVSRL SPLPP+++S NHA DCDDSL Y+YPF L+ VQKDG+ C+ C W  +CRGC + C  +     ++Y+A+DWD TALHLRY ++QER  +E  SV  S RA  E I+L  CL+AFT EEELGEDE YYCS CK+H+LA KK  LWRLPPIL                      IVH KRF +VNGRWIKS KIV FP + F+P  FLA   A++ 
Sbjct:    1 MGAKESRIGFLSYDEAVRRVTDVELKRLKDAFKR---------TSGLSAY---MTQQCFYKEVLGDGVPPKVAEVIYNSFGGTP------------KGLHFNNLIVGLVLLTRGRDEEKAKYIFSLFASDS-GSYVVREEVESRLHAVDGEVPP-SLRKCFLEGEKVNYDKFKNWLLQNKEAFTFSRWLLSGGVCVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLSACCRGPVAERQKFCFKVFDVDRDGVLSRDEMHKMVVALLEVWKDNRTDTLPELESSVSDIVEEILKIHD-TTKGHLTLEDYQIWSVKSALASEFLNLLFQVCHIVLGLRPATPKEEGQIIRGWLERESRHGLQPGQNWFLISMQWWKLWKDYVKYDNKS------IVVEQPGML--GSGRRTPQSTPSAEPAL---SKTGTVARSLSSPGEKSPKSNWEVSESTPNSQLPHVSSSATDICFARQHNVLDNNNQCFSTANGHLPNHLAAQRPGAIDNQPLVTTEPIKAPTLTMEGGRLKRPLNLMAGRDFETVPEPVWRALYHWYGANLSLPRPVIKSVITDQPELELFPRYLLFLRQQPATRTPQ-SNIWVNMGMSSLRMFPPHFPRGNMPSPNAPLKRMLAYTGCFSRMSTIKDIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHTLESLKIEDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTE----KGATGLSNLGNTCFMNSSIQCVSNTKPLTDYFITGRHLYELNRLNPIGMRGHMAKCYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDAEVATEAWDNHLRRNRSIVVDLFHGQLKSQVKCKTCGHISARFDPFNFLSLPLPMDSSMHLEITVIKLDGSTPVRYGLRLNMDEKYTGLKKQLSELCSLKPEQILLAEVHSSNIKNFPQDNQKVRLSVNGFLCAFEIPVPGSPTSITS-------------PAPVANTLMG----NGI---------------ARNSHSEK----------------------PGPVPNGTAGTPIQSGSDRSLANGM---PSTVMPSGSEKSLANGIPNGHATPVQDSPFTGYIIAMHRKMMRSELYFLSSQKNRPSLFGMPLIVPCTVHTSKKDLYDAVWIQVSRLASPLPPQEAS-NHAQDCDDSLGYQYPFTLRAVQKDGNSCAWCPWYRFCRGCKVECNEDRASLGNAYIAVDWDPTALHLRYQTSQERIVEEHTSVELSRRAQAEPISLDSCLKAFTSEEELGEDELYYCSKCKTHRLATKKLDLWRLPPIL----------------------IVHLKRFQFVNGRWIKSQKIVKFPCESFNPGAFLAPREAQST 1342          

HSP 2 Score: 110.153 bits (274), Expect = 3.100e-20
Identity = 45/99 (45.45%), Postives = 72/99 (72.73%), Query Frame = 0

Query: 1531 LEIDYNLYSMVCHSGVLAGGHYVSYGRHSDKKWYLQNDSACKPINEDSMDKSTAYLLFYEREGLCKSEYLPKIEAEKSLPDTRDLDEELETDLKKTCFI 1629
            LE  YNLY++ CHSG++ GGHYV+Y ++ + KWY  NDS+CK ++ + +D  +AY+LFYE++G+  S++LP  + +K + DT  +DE+ E+D KK C +
Sbjct: 1518 LEPVYNLYAISCHSGIMGGGHYVTYAKNPNDKWYCYNDSSCKEVHSEEIDTDSAYILFYEQQGVDYSQFLPVTDGKK-MADTTSMDEDFESDYKKYCVL 1615