BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000004974 (supercontig:LSalAtl2s:LSalAtl2s260:1491039:1496975:-1 gene:EMLSAG00000004974 transcript:EMLSAT00000004974 description:"maker-LSalAtl2s260-augustus-gene-14.34")

HSP 1 Score: 1359.36 bits (3517), Expect = 0.000e+0
Identity = 730/1281 (56.99%), Postives = 899/1281 (70.18%), Query Frame = 0

Query:   28 RKRISSETEYKSSPNERRQHQHVRFYPTNLR-----------RSMSEGYIKQPPTQLIGEYN-FHLESKRRHRSVESMYTYSRSESDGVGS-RSCSP---APSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQAC-------DPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKS-SKSRS------GSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELP-LMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTKSPNGIIEMDYSKLAQMVLRSSTGSKSNVSH----QAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNAVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLFQYSGSSSRPASLIGSSSLGSQHLPNVLLDGNQPGTSPNQSHMFSSSHADIRPRTKTEGGGGGLSSPANHHSPHFLALNNNYDEETSPKQTIKQKLTFKASLPGGSAKRPLAIKEEDDL 1273
            RKRI S + Y +S            +  N++           RSMSEG I +       +YN    E     ++  S ++  RS+S   G  R+  P     SS    +S+ DSPMQ D +T+S+GGLSD + HH  +S   +EPRSVMSGG+VKGWLPDVAVVLWRRMLGALGNIN IADTVIHAQIYKYLIEL+E++VKIRNNQGV+LDN  TPP P++VPP TIF PWC +AL+ P+ YQRG++YALRLLCL+T RPHD PL KTHL+QFYK+LH+GLT +K+D+MN LIK+TGPRFFSLMLPGYTAYILD+L+A N  ISTSELKGVPRTEATS+VG+LLAFP I+ DIP+LLPNS+ELSLISSAD+KDQI+G+LLKSG+KEPAGLARCISLSSLGIFVY+ELIH SFHPKIKEAIQVLLTALRFNNK+VAQIASDMLLLL DHV++ LD+YPEVPKKIVEVLA+TL  L P+ ++   EDEKRLL+SLLFCLGEW M+IP  +L+Q QEDG+SL Y VF ALQ A         P P G  E   RR  G +  +  + F   +  + S   M+ DFDPNIHVDNTKEGY S N+SP KS S+ +S       + E L +        DS  PVRLA+  +++HL+NH++HFPM  GAANLS L++E DD+PG   DEL M+ F  PNIQLF+LN++TL+SL+ELP +M++  +   IQT + QVR+ILRD+SGKFSWDS  + G    P+G+ +      +     SS  S   ++     Q +R+R  ++LP   N A+DMDNLDDLL Y+++TSPE  EE+G+P+N V  +  F+  VE D+IS V++QRT E+D+       L     P  P   E        FQ CR +F+ +G SSWE+RP++HLMKK ERL+RELKNLD  R RE HKIA+++V  GQEDK SIL N+SGSQAYEEF+SGLAW  ELE+HTGFM GL K+K+TGET PYYATSF+EVMFHVATRMPS +EES+LQKTRH+GNDEIHIVWSEH+RDYRRGILPTEFCDVLI+IYPL NKLFRIQ+SRK EVP+FGPLFNE IV  + LPGL+RATAINASRAKRSM+ ++QSHYEER+KALE+II NHK+ TT+EEFIT+V+SP  L NLFQ                                   PN     S    ++RPR+KTE      SS  +   P        Y   TSPK    +K++FK     G  K  +  +E DD+
Sbjct:  688 RKRIDSSSSYNNSLGSSNATTRTNDHSNNMKSKTSGRRKRMYRSMSEGNIVR------SDYNNIDDEIDGITKTFSSYFSKRRSKSVDFGIIRTSEPWGSRSSSPAPSSSINDSPMQFDPETMSDGGLSDLIGHHGSNSGGDNEPRSVMSGGSVKGWLPDVAVVLWRRMLGALGNINQIADTVIHAQIYKYLIELYEVIVKIRNNQGVTLDNMSTPPCPEYVPPVTIFTPWCFRALSXPDTYQRGRIYALRLLCLMTIRPHDNPLPKTHLIQFYKLLHQGLTQNKMDIMNILIKFTGPRFFSLMLPGYTAYILDYLYATNHIISTSELKGVPRTEATSIVGSLLAFPPIVRDIPVLLPNSNELSLISSADIKDQIVGILLKSGRKEPAGLARCISLSSLGIFVYTELIHKSFHPKIKEAIQVLLTALRFNNKSVAQIASDMLLLLTDHVQSLLDYYPEVPKKIVEVLARTLTGLAPKKDS---EDEKRLLMSLLFCLGEWTMKIPQQMLSQTQEDGQSLAYLVFNALQSAALSGCVSITPSPVGPTENNTRR-EGKNFEAFPQVFNDRSGTISSG--MLGDFDPNIHVDNTKEGYTSTNSSPQKSVSQPKSEHHLSRNNLEPLPLFSQNLSIMDSKCPVRLASNAIIAHLLNHVYHFPMGTGAANLSCLISEHDDVPGYVGDELSMEAFTSPNIQLFVLNDSTLVSLIELPVVMDDDPAFPGIQTGNSQVRIILRDLSGKFSWDSSILYG---PPDGLNKSMEDITSHPEFWSSCDSSFFLNKKRPCQPLRQRRASILPIYSNTADDMDNLDDLLSYLTYTSPEVMEEIGKPINTVSQSHFFDCVVEKDIISTVLNQRTSERDYTSNYGKNLLRPVSPPDPIGGEPK------FQFCRSIFNQMGWSSWEKRPHIHLMKKTERLIRELKNLDNIRGRETHKIAIIYVAAGQEDKHSILDNSSGSQAYEEFISGLAWXXELESHTGFMAGLXKNKSTGETXPYYATSFIEVMFHVATRMPSCTEESLLQKTRHLGNDEIHIVWSEHYRDYRRGILPTEFCDVLIIIYPLPNKLFRIQISRKLEVPFFGPLFNESIVDGKALPGLVRATAINASRAKRSMIPYFQSHYEERAKALEDIIKNHKESTTYEEFITKVYSPQTLQNLFQ-----------------------------------PNNPVRXSLXLQELRPRSKTEIQINRYSS-VSETDPL-------YQNGTSPKNM--KKMSFKNV--TGKVKTSVIKEENDDI 1900          

BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000009951 (supercontig:LSalAtl2s:LSalAtl2s64:61995:90540:1 gene:EMLSAG00000009951 transcript:EMLSAT00000009951 description:"maker-LSalAtl2s64-augustus-gene-1.7")

HSP 1 Score: 93.9745 bits (232), Expect = 1.460e-19
Identity = 68/237 (28.69%), Postives = 126/237 (53.16%), Query Frame = 0

Query:  852 LEQDHVKKLAPELTYSAHPEVPPQ-LEENQDY-PSVFQQCRRLFSHLGLSSWEQRPNVHLM---KKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKS-KTTGETAPYYATSFLEVMFHVATRMP-SLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKN-KLFRIQVSRKPEVPYFGP 1080
            L ++H + L  + + + H  +P   LEE ++Y PS  +  + L   L L       NVH +   + +E +++  +++  K+    +K  V++  +GQ  ++ + +N   S A+EEF+  L   V L  H GF GGL  +   TG+ + Y      E+MFHV+T +P + ++   LQ+ RHIGND + +V+ +    +   ++ + F    +V+ P+K+   +++ VS + +VP FGP
Sbjct:  312 LPENHTRVLIRQNSGTLHRLIPDSILEEEEEYGPSPLKIVQILLPELSLE------NVHPVVCPRSSELIVKYDEHVLVKQ----NKFGVIYQKQGQVTEEELFANREHSPAFEEFLDILGNRVLLTEHKGFRGGLDTTYGQTGDVSVYETFHNREIMFHVSTLLPYTDNDPQQLQRKRHIGNDIVALVFQDDETPFCPDMITSHFLHAYVVVRPIKDGSDYKVSVSARKDVPNFGP 538          

BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000002599 (supercontig:LSalAtl2s:LSalAtl2s151:403390:412004:-1 gene:EMLSAG00000002599 transcript:EMLSAT00000002599 description:"maker-LSalAtl2s151-augustus-gene-4.14")

HSP 1 Score: 93.5893 bits (231), Expect = 2.607e-19
Identity = 60/175 (34.29%), Postives = 90/175 (51.43%), Query Frame = 0

Query:  909 LMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQ-EDKQSILSNASGSQAYEEFVSGLAWEVEL---ETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESML-QKTRHIGNDEIHIVWSE---HWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEV 1075
            L+  N+     +KNLD     E HKI V+++G  Q  D+  IL N SGS  Y +F+ GL   + +   +  T F+GGL   +  G  A  +    ++ +FHVAT MP+   +    +K RHIGN+   IV+++   H   Y+ G +  +F    IVI PL     R+ V  KPE+
Sbjct: 1302 LLPSNKITENSIKNLDRIPAHETHKIGVLYIGPNQVNDEVGILRNISGSSRYTDFLDGLGNLINIRNIDKSTHFIGGLDXEEGDGNFAYMWXDDVMQXIFHVATLMPNHDNDPQANKKKRHIGNNYXAIVYNDSRNHSESYKMGTVRGKFISACIVITPLDQGSNRVCVDCKPEL 1476          

BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000009942 (supercontig:LSalAtl2s:LSalAtl2s64:423747:425954:-1 gene:EMLSAG00000009942 transcript:EMLSAT00000009942 description:"augustus_masked-LSalAtl2s64-processed-gene-4.0")

HSP 1 Score: 88.1965 bits (217), Expect = 6.788e-18
Identity = 51/155 (32.90%), Postives = 86/155 (55.48%), Query Frame = 0

Query:  932 HKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQ-KSKTTGETAPYYATSFLEVMFHVATRMPSLSEE-SMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLK---NKL-FRIQVSRKPEVPYFGP 1080
            +K  +V+   GQ  ++++  N S   A E F++ L  +V L +H G+ GGL  +   TG+ + +      E+MFHV+T +P    +   LQ+ RHIGND + I++ E    +   ++ + F    IV+ P++   NK+ ++I  S K +VPYFGP
Sbjct:  214 NKFGIVYQKVGQTTEEALFGNKSHCPAMETFLNLLGKKVMLXSHKGYRGGLDTQYGQTGKHSYFETFQGREIMFHVSTLLPXXDNDPQQLQRKRHIGNDIVAIIFQEGETPFSPDMITSHFLHAYIVVRPVQXSSNKVWYKIATSAKIDVPYFGP 368          

BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000009215 (supercontig:LSalAtl2s:LSalAtl2s586:192816:208940:-1 gene:EMLSAG00000009215 transcript:EMLSAT00000009215 description:"maker-LSalAtl2s586-augustus-gene-1.30")

HSP 1 Score: 79.7221 bits (195), Expect = 4.779e-15
Identity = 61/221 (27.60%), Postives = 102/221 (46.15%), Query Frame = 0

Query:  934 IAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGL-QKSKTTGETAPYYA-TSFLEVMFHVATRMP-SLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILP------TEFCDVLIVIYP----LKNKLFRIQVSRKPEVPYFGPLFNE--MIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFE 1139
            + +++  EGQ+ ++ + +N  G  A+ EF+  +  +V L+  + +  GL  K+ +TG  + Y    +  E+MFHV+T +P + S    L + R+IGND + +V+ E       G LP      ++F  V IV+       +N  + + VSR   VP FGP   E  M    +     +    INA  A      F       R + L+++  N    TT E
Sbjct:  465 VGLMYCREGQQTEEEMYNNEEGGAAFNEFLEMIGQKVRLKGFSKYKAGLCNKNDSTGLYSVYTEHENGSEIMFHVSTLLPFTNSNRQQLPRKRYIGNDIVTVVFQEP------GALPFIPNIKSQFQHVFIVVQAHNPCSENTQYSVAVSRSKSVPMFGPPIPEGAMFPKSKAFANFLLTKIINAENAAHHSDKFVSMAIRTRQELLKDLTTNFSSPTTLE 679          

BLAST of 250 kda substrate of akt vs. L. salmonis genes
Match: EMLSAG00000008967 (supercontig:LSalAtl2s:LSalAtl2s55:274420:296116:1 gene:EMLSAG00000008967 transcript:EMLSAT00000008967 description:"maker-LSalAtl2s55-snap-gene-3.15")

HSP 1 Score: 65.855 bits (159), Expect = 6.698e-11
Identity = 96/419 (22.91%), Postives = 177/419 (42.24%), Query Frame = 0

Query:  636 VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAEC------DDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTKSPNGIIEMDYSKLAQMVLRSSTGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNAVPTTTLFENEV-ESDVISGVMSQRT-LEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPN---------VHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQA----YEEFVSGLAWEVELETHTGFMGGL------QKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGN 1027
            V+ +A+++L+ ++    +FP   GA ++SSL+ E       +++P      L  +   + + + F+++N+ +++++E    E+ N ++E   S   V L+LR  SG+F+W S  +  L +  +G+ +  Y    + +     G K+  S +                 E +D +           P CK +   P  AV +    E    E D++S +M Q+  LE D + K     +     +      +N++    FQ  R + SHLGL S     +         + L  +++  + +L+ LD    R      + +V  GQ+    ILSN   S++    + EF++ L W V +  HTG+ G +      Q   T     P +         HV     +L E S +  T  +GN
Sbjct:  985 VKDSAESLLNCILEQTEYFPSPCGAESISSLMNEVSCLQHLNNLPNSTASSLTXEEAIK-HYRYFVVDNSVIVAILE----ESFNDMEERIPS---VTLLLRCSSGRFAWSS-QLRHLPRHKSGLNKSSYDNPGRPLPLGEMGIKTEHSPKFF--------------PESIDRI-----------PLCKADKSIP--AVESVAGDERAAQELDLLSHLMDQQAALENDIINKQQGINSGQFQLDTECIAPDNKND---FQTARLILSHLGLLSLNALQDNQDSLVPRLISLNAESQEFMCDLEGLDHLSSRTWDTAHIFYVARGQKTVDEILSNVESSESVHPHFLEFLASLGWPVNVWRHTGWTGHISTAWRCQXEPTKPPKIPNHGGGIFNGDHHVLYWADALQEVSFVVPT--LGN 1362          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|703556455|sp|O55007.2|RGPA1_RAT (RecName: Full=Ral GTPase-activating protein subunit alpha-1; AltName: Full=GAP-related-interacting partner to E12; Short=GRIPE; AltName: Full=GTPase-activating RapGAP domain-like 1; AltName: Full=Tuberin-like protein 1; AltName: Full=p240)

HSP 1 Score: 824.313 bits (2128), Expect = 0.000e+0
Identity = 464/1079 (43.00%), Postives = 667/1079 (61.82%), Query Frame = 0

Query:  103 GVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIH-------SSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQP--------QEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVS-DFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNS---VQEIQTSSCQVRLILRDISGKFSWDSCAM------NGLTKSPNGIIEMDYSKLAQMVLRSS-----TGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNA-VPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAP 1150
             +GSRS +P+PS+ L    M+   +QLD          ++L H  +        S    E  SVM+GG + GW  DVA V+WRRMLG LG++N I D  IHAQ++ YL EL + + KIR+N G+S DN  +P PP  +PP  I  PW  KA  L + Y++GKL+A +L+C    R  D   ++  L  FY ++H GL     D++NT+IK+  P+FFSL LPG T  I+DF+ AA   +++S     PR EA  ++G+L+ FP++  ++P L P++ ++++    DVK+ II  +L S + EP+G ARC++L SLGI++  EL+H S HP+IKEA+ V+  +L+F NK VA +A +ML +L  +      + P+ P KI+++L  T+  L P  EA   E +KRL++SLL CL +W M +P   L Q         ++  KS+L  +++ L   C  G Q                     F       +S + + S D+DP +H+++ +E        PL S  S   S+    + +V++      I +  AA+TV++HLVNHL H+PMS G A L+S V E  D     + EL  ++F  PNIQ F+LNNTTL+S +++   E+V        + +++  VR+I+RD+SGK+SWDS  +      +GL +  + I+ M Y +  +    S+        K  +S Q +RR  ETV PT     E+ D LD+LLQY+  TSPEC +  G  LN   P       + E+DVI+ ++ Q T E++ V+K   +L   A  +  P  ++ Q   S F  CR L S LG++SW++R + HL+KKNE+LLREL+NLD+++CRE HKIAV +V EGQEDK SIL+N  GSQAYE+FV+GL WEV L  H GFMGGLQK+K+TG T PY+ATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PTEF DVLIVIYP+KN +F IQ+ +KPEVP+FGPLF+  IV  +VLP ++R+TAINASRA +S++  YQ+ YEER++ L+ I+ +H + TTFE+F  QVFSPAP
Sbjct:  994 ALGSRSQTPSPST-LNIDHMEQKDLQLD----------EKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIIAAG-RVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPSTPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYAPRLQTYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQSVHSTGAENEKTEKSVLNCIYKVLH-GCVYGAQS--------------------FSHPKYFPISLSDLASVDYDPFMHLESLRE------PEPLHSPDSERSSK-LQPVTEVKTQMQQGLISI--AARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFDSPNIQFFVLNNTTLVSCIQIRSEESVPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVSGLPEPTSFILSMSYQEKPEEPPTSNECLEDITVKDGLSLQ-LRRFRETV-PTWSTIREEEDVLDELLQYLGTTSPECLQRTGISLNVPAPQPVCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQKPQ---SAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSESDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNLYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAP 2025          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|51315850|sp|Q6GYQ0.1|RGPA1_HUMAN (RecName: Full=Ral GTPase-activating protein subunit alpha-1; AltName: Full=GAP-related-interacting partner to E12; Short=GRIPE; AltName: Full=GTPase-activating Rap/Ran-GAP domain-like 1; AltName: Full=Tuberin-like protein 1; AltName: Full=p240)

HSP 1 Score: 823.157 bits (2125), Expect = 0.000e+0
Identity = 466/1082 (43.07%), Postives = 671/1082 (62.01%), Query Frame = 0

Query:  104 VGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIH-------SSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQP--------QEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVS-DFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENV---NSVQEIQTSSCQVRLILRDISGKFSWDSCAM------NGLTKSPNGIIEMDYSKLAQMVLRSS-----TGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNA-VPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNL 1154
            +GSRS +P+PS+ L    M+   +QLD          ++L H  +        S    E  SVM+GG + GW  DVA V+WRRMLG LG++N I D  IHAQ++ YL EL + + KIR+N G+S DN  +P PP  +PP  I  PW  KA  L + Y++GKL+A +L+C    R  D   ++  L  FY ++H GL     D++NT+IK+  P+FFSL LPG T  I+DF+ AA   +++S     PR EA  ++G+L+ FP++  ++P L PN  ++++    DVK+ II  +L S + EP+G ARC++L SLGI++  EL+H S HP+IKEA+ V+  +L+F NK VA +A +ML +L  +V     + P+ P KI+++L  T+  L P  EA   E +KRL++SLL CL +W M +P   L QP         +  KS+L  +++ L   C  G Q                     F       +S + + S D+DP +H+++ KE        PL S  S   S+    + +V++      I +  AA+TV++HLVNHL H+PMS G A L+S V E  D     + EL  ++F+ PNIQ F+LNNTTL+S +++   EN+        + +++  VR+I+RD+SGK+SWDS  +      +GL++  + ++ + + +  +    S+        K  +S Q  R R ETV PT +   ++ D LD+LLQY+  TSPEC +  G  LN   P       + E+DVI+ ++ Q T E++ V+K   +L   A  +  P  ++ Q   S F  CR L S LG++SW++R + HL+KKNE+LLREL+NLD+++CRE HKIAV +V EGQEDK SIL+N  GSQAYE+FV+GL WEV L  H GFMGGLQK+K+TG T PY+ATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PTEF DVLIVIYP+KN +F IQ+ +KPEVP+FGPLF+  IV  +VLP ++RATAINASRA +S++  YQ+ YEER++ L+ I+ +H + TTFE+F  QVFSPAP  +L
Sbjct:  996 LGSRSQTPSPST-LNIDHMEQKDLQLD----------EKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAG-RVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPSLHPNIPDVAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPFHATGAESDKTEKSVLNCIYKVLH-GCVYGAQC--------------------FSNPRYFPMSLSDLASVDYDPFMHLESLKE------PEPLHSPDSERSSK-LQPVTEVKTQMQHGLISI--AARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEENMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPPVSGLSEPTSFMLSLSHQEKPEEPPTSNECLEDITVKDGLSLQFKRFR-ETV-PTWDTIRDEEDVLDELLQYLGVTSPECLQRTGISLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAVEQDEPIPQKPQ---SAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHL 2030          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|51315849|sp|Q6GYP7.1|RGPA1_MOUSE (RecName: Full=Ral GTPase-activating protein subunit alpha-1; AltName: Full=GAP-related-interacting partner to E12; Short=GRIPE; AltName: Full=GTPase-activating RapGAP domain-like 1; AltName: Full=Tuberin-like protein 1; AltName: Full=p240)

HSP 1 Score: 820.076 bits (2117), Expect = 0.000e+0
Identity = 464/1079 (43.00%), Postives = 666/1079 (61.72%), Query Frame = 0

Query:  103 GVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIH-------SSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQP--------QEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVS-DFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNS---VQEIQTSSCQVRLILRDISGKFSWDSCAM------NGLTKSPNGIIEMDYSKLAQMVLRSS-----TGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNA-VPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAP 1150
             +GSRS +P+PS+ L    ++   +QLD          ++L H  +        S    E  SVM+GG + GW  DVA V+WRRMLG LG++N I D  IHAQ++ YL EL + + KIR+N G+S DN  +P PP  +PP  I  PW  KA  L + Y++GKL+A +L+C    R  D   ++  L  FY ++H GL     D++NT+IK+  P+FFSL LPG T  I+DF+ AA   +++S     PR EA  ++G+L+ FP++  ++P L PN  ++++    DVK+ II  +L S + EP+G ARC++L SLGI++  EL+H S HP+IKEA+ V+  +L+F NK VA +A +ML +L  +V       P+ P KI+++L  T+  L P  EA   E +KRL++SLL CL +W M +P   L QP         +  KS+L  +++ L   C  G Q                     F       +S + + S D+DP +H+++ KE        PL S  S   S+    + +V++      I +  AA+TV++HLVNHL H+PMS G A L+S V E  D     + EL  ++F+ PNIQ F+LNNTTL+S +++   E++        + +++  VR+I+RD+SGK+SWDS  +      +GL +  + I+ M   +  +    S+        K  +S Q +RR  ETV PT     E+ D LD+LLQY+  TSPEC +  G  LN   P       + E+DVI+ ++ Q T E++ V+K   +L   A  +  P  ++ Q   S F  CR L S LG++SW++R + HL+KKNE+LLREL+NLD+++CRE HKIAV +V EGQEDK SIL+N  GSQAYE+FV+GL WEV L  H GFMGGLQK+++TG T PY+ATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PTEF DVLIVIYP+KN +F IQ+ +KPEVP+FGPLF+  IV  +VLP ++R+TAINASRA +S++  YQ+ YEER++ L+ I+ +H + TTFE+F  QVFSPAP
Sbjct:  994 ALGSRSQTPSPST-LSRAHIEQKDLQLD----------EKLHHSVLQTPDDLEISEFPSECCSVMAGGTLTGWHADVATVMWRRMLGILGDVNAIMDPEIHAQVFDYLCELWQNLAKIRDNLGISADNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIIAAG-RVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPALHPNIPDIAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQIHQPQSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMALPLKTLLQPVHATGAENDKTEKSVLNCIYKVLH-GCVYGAQS--------------------FSNPKYFPISLSDLASVDYDPFMHLESLKE------PEPLHSPDSERSSK-LQPVTEVKTQMQQGLISI--AARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEESMPGGGLAAGLVSANSNVRIIVRDLSGKYSWDSAILYGPPIVSGLPEPTSFILSMSDQEKPEEPPTSNECLEDIAVKDGLSLQ-LRRFRETV-PTWSTIREEEDVLDELLQYLGTTSPECLQRTGISLNVPAPQPLCISEKQENDVINAILKQYTEEKEFVEKHFNDLNMKASEQDEPTPQKPQ---SAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQEDKYSILTNIGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNRSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRSTAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAP 2025          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|147742932|sp|Q2PPJ7.2|RGPA2_HUMAN (RecName: Full=Ral GTPase-activating protein subunit alpha-2; AltName: Full=250 kDa substrate of Akt; Short=AS250; AltName: Full=p220)

HSP 1 Score: 771.541 bits (1991), Expect = 0.000e+0
Identity = 430/1014 (42.41%), Postives = 614/1014 (60.55%), Query Frame = 0

Query:  158 SVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLL--LPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQP--------QEDGKS-LLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP-VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTK---SPNGIIEMDYSKLAQMVLRSSTGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNAVPTTTLFEN-EVESDVISGVMSQRTLEQDHVKK----LAPELTYSAHPE-VPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAP 1150
            S+++GG++ GW PD A VLWRR+LG LG++N+I    IHA+++ YL EL   + KIR+N  +SLDN  +P PP  +PP  +FA W  KA  LP  Y+ GKL A RL+C +  R  D   +   LV FY V+H GLT    D++NT+I++  PRFFSL  PG++  + DF+ AA   +ST  L   PR+EA +V+G+L+ FP+  ++IPLL  +P  +E ++  + DVK  +I +LLK+  +EP   ARCI++ SLG+++  EL   + HP++KEAI V+   L+F NK VAQ+A D+L LL  + +    F   +P+K+ E+L  T+  L P  E    E +K+ ++SLL CL +WCM +P  +L  P        Q   ++ LL +++  L   C  G               S+T T++       A LS     +D+DP + + N K      ++ P++       S E  +++ V       ++  + L A+ V++HLVNHL H+P+S G A L SLV+E  D   +   EL  +VF+ PN+QLF+ N++TL+S ++ P    V        S   VR+I+RDISGK+SWD   + G  +   +PNG    + S L     R + G + + S                   E  D LD LL+ I HTSPEC       LN    T    N + E ++I  ++ Q   E ++++      A ++T    P  V P+          F  CR L   LG++SW++R N HL+KKN +LLRELKNLD+++CRE HKIAV ++ EGQEDK SILSN  GSQAYE+FV+GL WEV+L TH GFMGGLQ++ +TG+TAPYYATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PT F DV I+IYP+KN +F I +++KPEVP+FGPLF+  IV  ++LP L+ AT INASRA + ++  YQS YEER+  LE II NH++  TFE+F  QVFSP+P
Sbjct:  913 SIIAGGSLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHARVFCYLYELWYKLAKIRDNLAISLDNQSSPSPPVLIPPLRMFASWLFKAATLPNEYKEGKLQAYRLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGFPGFSMLVGDFITAAARVLSTDILTA-PRSEAVTVLGSLVCFPNTYQEIPLLQSVPEVNE-AITGTEDVKHYLINILLKNATEEPNEYARCIAVCSLGVWICEELAQCTSHPQVKEAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETSLPRKMAEILVATVAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSVLLHPVSTAVLEEQHSARAPLLDYIYRVLH-CCVCG---------------SSTYTQQSHYILTLADLSS----TDYDPFLPLANVK------SSEPVQ----YHSSAELGNLLTVEEEKKRRSLELIPLTARMVMAHLVNHLGHYPLSGGPAILHSLVSENHDNAHVEGSELSFEVFRSPNLQLFVFNDSTLISYLQTPTEGPVGGSP--VGSLSDVRVIVRDISGKYSWDGKVLYGPLEGCLAPNG---RNPSFLISSWHRDTFGPQKDSSQ----------------VEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSLTPCGMNYDQEKEIIEVILRQNAQEDEYIQSHNFDSAMKVTSQGQPSPVEPR--------GPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFIAITKKPEVPFFGPLFDGAIVSGKLLPSLVCATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSP 1865          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|147742933|sp|A3KGS3.2|RGPA2_MOUSE (RecName: Full=Ral GTPase-activating protein subunit alpha-2; AltName: Full=250 kDa substrate of Akt; Short=AS250; AltName: Full=P220)

HSP 1 Score: 756.133 bits (1951), Expect = 0.000e+0
Identity = 429/1005 (42.69%), Postives = 605/1005 (60.20%), Query Frame = 0

Query:  158 SVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSA-DVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGH-----SNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTK---SPNGIIEMDYSKLAQMVLRSSTGSKSNVSHQAMRRRNETVLPTQE--NAAEDMDNLDDLLQYISHTSPECKEEVGRPLNA-VPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSAHPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAP 1150
            S+++GG + GW PD A VLWRR+LG LG++N+I    IHA+++ YL EL   + KIR+N  +SLDN  +P PP  +PP  +FA W  KA  LP  Y+ GKL A +L+C +  R  D   +   LV FY V+H GLT    DV+NT+IK   PRFFSL LPG++  + DF+ AA   +ST  L   PR+EA +++G+L+ FP+  ++IPLL        +++ A DVK  +I +LLK+  +EP   ARCI++ SLG+++  EL   + HP++K+AI V+   L+F NK VAQ+A D+L LL  + +    F   +P+K+ E+L  T+  L P  E    E +K+ ++SLL CL +WCM +P   L  P          V E L       P  AP +       H     S+T T++   T   A LS     +D+DP + + N +      N+ P++   S +     L++ + +       IP  L A+ V++HLVNHL H+P+S G A L SLV+E  D   +   EL  +VF+ PN+QLF+ N++TL+S ++ P             S   VR+I+RDISGK+SWD   + G  +   +PNG                      N S Q     + T  P ++  N  E  D LD LL+ I HTSPEC       LN   PT      + E  ++  ++ Q   E ++V++   + + +   +  P   E +     F  CR L   LG++SW++R N HL+KKN +LLRELKNLD+++CRE HKIAV ++ EGQEDK SIL+N  GSQAYE+FV+GL WEV+L TH GFMGGLQ++ +TG+TAPYYATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PT F DV I+IYP+KN +F I +++KPEVP+FGPLF+  IV  ++LP LI AT INASRA + ++  YQS YEER+  LE II NH++  TFE+F  QVFSP+P
Sbjct:  912 SIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFGYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDML-AAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQSASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTA------VLEELH------PSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSS----TDYDPFLPLANVR------NSEPIQYHSS-ADLGNLLTVEEEKKRRSVELIP--LTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTS--GGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPNG---------------------RNPSFQISGWHHHTCGPQKDLFNGEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPCAMNWDQEKAIMEVILRQSAQEDEYVQRCNSDSSVTVTSQGQPSPVEPR---GPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSP 1864          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|284022067|sp|P86411.1|RGPA2_RAT (RecName: Full=Ral GTPase-activating protein subunit alpha-2; AltName: Full=250 kDa substrate of Akt; Short=AS250; AltName: Full=p220)

HSP 1 Score: 755.747 bits (1950), Expect = 0.000e+0
Identity = 450/1099 (40.95%), Postives = 640/1099 (58.23%), Query Frame = 0

Query:   75 GEYNFHLESKRRH-RSVESMYTYSRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPR-------SVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSA-DVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGH-----SNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTK---SPNGIIEMDYSKLAQMVLRSSTGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNA-VPTTTLFENEVESDVISGVMSQRTLEQDHVKKL----APELTYSAHPE-VPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAP 1150
             E +   ES++ H R  E   + S      VG  + +  P S  + T  +D  +    D +++      L      +S   E R       S+++GG + GW PD A VLWRR+LG LG++N+I    IHA+++ YL EL   + KIR+N  +SLDN  +P PP  +PP  +FA W  KA  LP  Y+ GKL A +L+C +  R  D   +   LV FY V+H GLT    DV+NT+IK   PRFFSL LPG++  + DF+ AA   +ST  L   PR+EA +++G+L+ FP+  ++IPLL        +++ A DVK  +I +LLK+  +EP   ARCI++ SLG+++  EL   + HP++K+AI V+   L+F NK VAQ+A D+L LL  + +    F   +P+K+ E+L  T+  L P  E    E +K+ ++SLL CL +WCM +P   L  P          V E L       P  AP +       H     S+T T++   T   A LS     +D+DP + + N +      N+ P++   S +     L++ + +       IP  L A+ V++HLVNHL H+P+S G A L SLV+E  D   +   EL  +VF+ PN+QLF+ N++TL+S ++ P             S   VR+I+RDISGK+SWD   + G  +   +P+G    + S         + G +SN+ H                  E  D LD LL+ I HTSPEC       LN   PT +    + E ++I  ++ Q T E ++V++     A ++T    P  V P+          F  CR L   LG++SW++R N HL+KKN +LLRELKNLD+++CRE HKIAV ++ EGQEDK SIL+N  GSQAYE+FV+GL WEV+L TH GFMGGLQ++ +TG+TAPYYATS +EV+FHV+TRMPS S++S+ +K RH+GNDE+HIVWSEH RDYRRGI+PT F DV I+IYP+KN +F I +++KPEVP+FGPLF+  IV  ++LP LI AT INASRA + ++  YQS YEER+  LE II NH++  TFE+F  QVFSP+P
Sbjct:  822 AELDLKEESQQTHGRCRERQKSESTGSDMAVGYSNEAELPVSPWQ-TCEEDPELSTPTDAVADSDARHWLQLSPTDASNLTESRECLADDCSIIAGGNLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHAKVFSYLYELWYKLAKIRDNLAISLDNQSSPSPPLLIPPLRMFASWLFKATTLPNEYKEGKLQAYKLICAMMTRRQDVLPNSDFLVHFYLVMHLGLTSEDQDVLNTIIKNCSPRFFSLGLPGFSMLVGDFITAAARVLSTDML-AAPRSEALTLLGSLVCFPNTYQEIPLLQSVPEVSDVVTGAEDVKHYLINILLKNATEEPNECARCIAICSLGVWICEELAQCASHPQVKDAINVIGVTLKFPNKIVAQVACDVLQLLVSYWEKLQMFETALPRKMAEILVATIAFLLPSAEYSSVETDKKFIVSLLLCLLDWCMALPVSALLHPVSTA------VIEELH------PSRAPLLDYIYRVLHCCVCGSSTYTQQSHYTLTLADLSS----TDYDPFLPLANVR------NSEPVQYHSS-ADLGNLLTVEEEKKRRSVELIP--LTARMVMAHLVNHLGHYPLSGGPAVLHSLVSENHDNAHVEGTELSSEVFRSPNLQLFVFNDSTLISYLQTPAEGPAGGTS--GGSLSDVRVIVRDISGKYSWDGKVLYGPLEGRLAPSG---RNPSFQISGWHHHTCGPQSNLFH----------------GEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSPTPSAMNCDQEKEIIEVILRQSTQEDEYVQRCHSNSAVKVTSQGQPSPVEPR--------GPFYFCRLLLDDLGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILANERGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFITITKKPEVPFFGPLFDGAIVSGKLLPSLICATCINASRAVKCLIPLYQSFYEERALYLEAIIQNHREVMTFEDFAAQVFSPSP 1864          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|74868143|sp|Q9VB98.2|Y5521_DROME (RecName: Full=Probable Rho GTPase-activating protein CG5521)

HSP 1 Score: 728.398 bits (1879), Expect = 0.000e+0
Identity = 452/1170 (38.63%), Postives = 660/1170 (56.41%), Query Frame = 0

Query:   51 RFYPTNLRRSMSEGYIKQPPTQLIGEYNFHLESKRRHRSVESMYTYSRSESD-GVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKY-TGPRFFSLMLPGYTAYILDFLFAANCTISTSEL-KGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELI--------------------HGSFHPK--------IKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQP-QEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNI-HVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGL---GTDELCMDVFQQP------NIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM----NGLTKSPNGIIEMDYSK-LAQMVLRSSTGSKSNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNAVPTTTLFENEVESDVISGVMSQRTLEQDHV----KKLAPELTYSAHPEVPPQLEENQDYPSV-------------FQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNL 1154
            R  P +LRR+MS                  L+S  R    E   +Y   +++ G GSRS SP  SSG+E  S+KD+ +Q+D    S    +        +S +    R ++ GG+ +GW PD   ++W+RMLGALG++N I    +HAQ++ +L+E+ + ++KI+ NQG+S DN  T P P  VPP  I APWC  +L+L  ++++GKL+AL+LLC L  +     ++   L  FY  LH+ LTG   D++  ++K+  GPR  SL+LPG+T  +LD + A+   +++ E+ +  PR E  +++G+LL +PS L    +L P   +  L+  +D++D I+ ++L+  ++EP+  ARCI+LS LG ++   L                     H   HPK        IKE +QVLL AL+F +  +A +A D L L A+  +  L     VP+ I+  + K   +L  +      + +K +L SL+ CLGE+CM IP  I+  P  E G +L+  V   L Q       GAP         H         +TA            DFD +I H D   +G +     P  + ++    Q+ ++ I             +L AK V  HLV H+ HFPM  GA+ LSS+V E DDI      G  E   D  + P      N+QLF+LN+  + S +ELP ++         + T+  QVR+++RD++GK  WD+  +        + P    ++ +S+ L  M     T + S      +R R   VLP  ++ A D+D LDD+L YI HTSPEC       LNA PT +      E+  IS +++QR LEQ+ V    +  +P L +++      Q ++   + +              FQ CR LFSHLGL+ WE+R   HL++++E+L+REL+N+D ++CRE HK+AV++V  GQEDK SIL N SGS  YE FVS L WE++LETH GF+GGL + +  G TAPYYAT FLEV++HVATRMPS S E+ML KTRH+GNDE+HIVWSEH RDYRR ILPTEFCDVLIV+YPL+N LFR+ V+RKPEVP+FGPL NE +V    L  LIRATAINASR KR+ L  YQ  YEER+++L+++ + +K+ TTFE+F +++++P PL+ L
Sbjct:  753 RINPQDLRRAMS------------------LDSLARKGDAEETDSYQEGDNESGAGSRSPSPTASSGIEGGSIKDAQLQID---ASLDDANSGSYGSGSNSGSISGRRCIILGGSAEGWHPDSTSIMWKRMLGALGDVNRIPKADLHAQVFMHLLEMTQNLIKIKQNQGISTDNQNTQPMPPLVPPIGIVAPWCYGSLSLDRSFKKGKLWALQLLCSLAIQ---GAVNMQQLPLFYHALHQLLTGEDRDLIYAILKHLEGPRLLSLLLPGHTLLLLDLVHASAILLTSLEVSRSTPRAEVAALLGSLLCYPSSLLTRSVLQPTPQKFELMECSDLQDHILNIVLRCARREPSAKARCIALSQLGQWLLMRLSQPLPASNSGRANLFQQAVPHHKDVHPKASSVYNPRIKEVLQVLLQALQFKHHTIAIVAVDSLKLCAERGRQ-LAAIERVPQLIITAICK---ALEIQSVTKPKDSDKVVLTSLMLCLGEFCMAIPAPIMLTPFNEQGDTLVLQVLRVLLQVAS----GAPR--------HERVK-----LTAD----------DDFDMHIAHDDLQGDGRL-----PEATYQTSETIQKCITAI-------------KLCAKAVSMHLVTHIGHFPMGIGASRLSSMVEEQDDIGNAAYGGQVETRRDSVELPSVVSAQNMQLFMLNSGLVASFIELPTLKLPGGGITAGLVTADKQVRVLMRDLNGKACWDASILYSEPRNAEEPPKTTPKIQHSQPLDSMATSMVTHTPS--PRHTLRHRPAGVLPLAKDMAPDLDQLDDMLAYIGHTSPECVAPTVSQLNA-PTASPLSGNQEAQAISVILNQRLLEQEFVTHSTQAPSPALRHASSNSSLQQPDQRSLHSTTASFDSLPTRTEMPFQYCRLLFSHLGLAGWERRSRTHLLQRSEKLMRELRNVDLQKCRETHKMAVIYVAAGQEDKGSILRNTSGSSTYEMFVSALGWEIDLETHNGFLGGLPR-QGCGATAPYYATPFLEVVYHVATRMPSDSSEAMLLKTRHLGNDEVHIVWSEHNRDYRRDILPTEFCDVLIVVYPLRNGLFRVTVNRKPEVPWFGPLANESVVSGACLATLIRATAINASRTKRAALPLYQQFYEERNRSLDSVSSRYKESTTFEDFASRIYNPMPLSTL 1845          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|74855334|sp|Q54TK4.1|Y1809_DICDI (RecName: Full=Rap-GAP domain-containing protein DDB_G0281809)

HSP 1 Score: 134.42 bits (337), Expect = 2.422e-30
Identity = 90/307 (29.32%), Postives = 157/307 (51.14%), Query Frame = 0

Query:  866 YSAHPEV---PPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASG--SQAYEEFVSGLAWEVELETHTGFMGGLQKSKTT-GETAPYYATSFLEVMFHVATRMPS---LSEESMLQKTRHIGNDEIHIVW-SEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKP---EVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLT---FYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLFQ 1156
            YS  P +   PP L+ + +     QQ R L +HLG  + E R  +  ++ + +  + L  LD+   R   KI +++V +G   +  I +N +   +Q Y++F++ L W V + THTGF+G L K   T G+  PYYAT   E++F V+T MP+    +  S   K + I    + ++W +     Y + +L T    + IVI PL+N LFR++  RK         GP+ +E+I+ + +L  +IR + +N++++  ++ +     Q  Y  R K + +I  + K   T ++F    F P   + L+Q
Sbjct: 1216 YSLTPSITTHPPVLKTSFNGDCKLQQARILLTHLGFLNQENRNKLTPLENSVQFFQSLNMLDSVSERVQIKIPIIYVKKGDSTEDDIYNNVTSNTTQDYQDFIASLGWLVPISTHTGFLGDLDKKNLTHGQFTPYYATHSREMVFFVSTMMPNSDIANNSSQEHKKKLINKTNVSVIWFNGSIEVYEKTLLETFPHAIQIVITPLENDLFRLKTLRKATHSNRMKTGPVNDEIIISKHILANVIRLSVVNSNQSLLNLSSGDGKTQHIYTNRKKLISDITESFKHDMTIQKFYEFHFEPLDQSQLYQ 1522          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|1717800|sp|P49816.1|TSC2_RAT (RecName: Full=Tuberin; AltName: Full=Tuberous sclerosis 2 protein homolog)

HSP 1 Score: 97.0561 bits (240), Expect = 7.909e-19
Identity = 62/208 (29.81%), Postives = 108/208 (51.92%), Query Frame = 0

Query:  906 NVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQ-SILSNASGSQAYEEFVSGLAWEVEL---ETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLS-EESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKR 1108
            N  ++  NE   R ++ LD     + HKIAV++VGEGQ   + +ILSN  GS  Y EF++GL   +EL   +    ++GGL      G+    +    ++ +FH+AT MP+   ++    K RH+GND + I++++   D++ G +  +F  V ++I PL  K   + +  + ++          IV  R L  + R  A++A+ A +
Sbjct: 1519 NKPILLPNESFERSVQLLDQIPSYDTHKIAVLYVGEGQSSSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIIYNDSGEDFKLGTIKGQFNFVHVIITPLDYKCNLLTLQCRKDMEGLVDTSVAKIVSDRNLSFVARQMALHANMASQ 1726          

BLAST of 250 kda substrate of akt vs. SwissProt
Match: gi|269849475|sp|P49815.2|TSC2_HUMAN (RecName: Full=Tuberin; AltName: Full=Tuberous sclerosis 2 protein)

HSP 1 Score: 96.6709 bits (239), Expect = 9.768e-19
Identity = 59/196 (30.10%), Postives = 105/196 (53.57%), Query Frame = 0

Query:  918 RELKNLDAKRCREAHKIAVVFVGEGQEDKQ-SILSNASGSQAYEEFVSGLAWEVEL---ETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLS-EESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKR 1108
            R ++ LD     + HKIAV++VGEGQ + + +ILSN  GS  Y EF++GL   +EL   +    ++GGL      G+    +    ++ +FH+AT MP+   ++    K RH+GND + IV+++   D++ G +  +F  V +++ PL  +   + +  + ++          IV  R LP + R  A++A+ A +
Sbjct: 1529 RSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQ 1724          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1325277893|ref|XP_023323584.1| (LOW QUALITY PROTEIN: probable Rho GTPase-activating protein CG5521 [Eurytemora affinis])

HSP 1 Score: 1172.92 bits (3033), Expect = 0.000e+0
Identity = 680/1297 (52.43%), Postives = 886/1297 (68.31%), Query Frame = 0

Query:   23 SPYPRRKRISSETEYKSSPNERRQHQHVRFYPTNL---RRSMSEGYIKQPPTQLIGEYNFHLES-----KRRHRSVESMYTYSRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSD--QLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAG--VNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTK-EGYMSNNTSPLKSSKSRSGSQE-----ALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLMENVNSVQEIQTSSCQVRLILRDISGKFSWDSCAMNGLTKSPNGIIEMDYSKLAQMVLRSSTGSKSNV---SHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLNAVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLA-PELTYSA-HPEVPPQ---LEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLFQYSGSSSRPASLIGSSSLGS---QHLPNVLLDGNQPGTSPNQSHMFSSSHADIRPRTKTEGGGG-------------GLSS---PANHHSPHF----LALNNNYDEETSPKQTIKQKLTFKASLPGGSA-KRPLAIKE 1269
            SPYP R RISSE+E   +P       H+ F    L   +R MSE +I  P  Q   +++  L +     +RR RSV+S+        D   SRS SP  SS +E  S+KDSP+QLDHD   EG   D   +SH +        P+SVM+GG+VKGWLPDVAVVLWRRMLG+LG++N IADTVIHAQIYKYLI+LHEIM KIR NQGVS+DN  TP  P +VPPFTIFAPWC +AL LP+ YQRG++YALRLLCLLT RP DTPL +THL+QFYKVLH+GL  +  D ++ L+++TG RFFSL LPGY++++ D+L A+N  IS+ E KG+PRTEA S+VG+LL++PS++  +PLLLP+S +LSL+SS D++DQ+I VLLK+GKKEP GLARCI+LSSLG FVY EL+H ++H K+K+AI VLL AL+FNNKAVAQ+ASDML+L+++ V   L ++ ++PK+IVEVL+++L SL P+ E    ++++++RLLLSLL CLGEWCM++P  +LTQ QEDGKSLL HVFEAL     P               HSN S+    +T+     SP   V  FDP+IHVDN + E +   N SP K  KSRS S++     +L ++Q    D      V+LAA+T+LSHLVNHL HFP + GA++LSS V+E DD+P L +DEL ++ FQ PNIQLFILNNTTL+SLVE+P +E    +    T+  QVR+ILRDISGKF WD+  +   +   N  +   +S +       ST   + V        R R+   LPT E +A+D+D LDDLLQY+ HTSPE       PLN+VP     +N +E D+IS V+SQR LE D + +L+ P   Y+A  P +PP+   L E +     FQQ R LF  LGL SWE+RP+VHL+KK E+L+RELKNLD ++ RE HKIAV+FV EGQEDK SILSN  GS+A+EEFV+GL WEVELETHTGF+GGL ++K+TGETAPYYATSFLE+MFHV+TRM S SE+ MLQKTRH+GNDEIHIVWSEHWRDYRRGI+PTEFCDVLIVIYPLKNKL+RIQV RK EVP+FGPL+NEMIV   VLPGLIRATA+NASRAKR+M+T Y++++E+R+KAL++II   K  TTFEEF+  VF+PA    + Q    + RP  +  S+   +     L  VLLD     T  ++      +H ++  RT++  G                LSS   P +H +P      + L++  D    P  + + K + K+ +   SA +RP+A+++
Sbjct:  598 SPYPPRHRISSESEGIGAP---YTEDHLEFSERMLFTRKRCMSESWINFPRNQ---DHDIMLAAAWRLKQRRSRSVDSL-------RDRESSRSPSPELSSSMESHSIKDSPIQLDHD--HEGAFYDIGDVSHGS------QGPKSVMAGGSVKGWLPDVAVVLWRRMLGSLGDVNSIADTVIHAQIYKYLIDLHEIMNKIRANQGVSVDNLKTPESPQYVPPFTIFAPWCFRALLLPDTYQRGRMYALRLLCLLTVRPQDTPLPRTHLIQFYKVLHQGLCSNDQDTVHNLVRFTGSRFFSLSLPGYSSFLYDYLTASNSVISSQEFKGLPRTEAVSIVGSLLSYPSVVSSLPLLLPSSDQLSLVSSQDLQDQVISVLLKAGKKEPNGLARCIALSSLGTFVYRELVHQTYHSKLKDAIIVLLAALKFNNKAVAQVASDMLMLISEQVDRVLAYHQDLPKRIVEVLSRSLASLLPKPEVSNDISKEDRRLLLSLLSCLGEWCMKLPLEVLTQIQEDGKSLLNHVFEALLSGIKP--------------AHSNLSS--PLLTSN----SPVGYVDGFDPDIHVDNLRGENW---NASPAK-LKSRSASRDLMDDASLPLLQ----DEGPGAVVQLAARTLLSHLVNHLNHFPQAVGASSLSSQVSEQDDVPTLVSDELNLETFQSPNIQLFILNNTTLLSLVEIPGLEEDVEMSGFITAPSQVRIILRDISGKFVWDTSVLYTPSSMQNQSLAPSWSGV-------STPQHATVLSPPRHTKRHRDPEELPTHEVSAQDLDQLDDLLQYLGHTSPELLSMPNLPLNSVPPWHGVDNTLEGDIISSVISQRNLELDQLSRLSGPHSLYNAGKPVLPPETPVLREEE----CFQQARILFQQLGLCSWEKRPHVHLVKKTEQLVRELKNLDNQKGRETHKIAVIFVAEGQEDKISILSNTGGSEAFEEFVAGLGWEVELETHTGFIGGLTRNKSTGETAPYYATSFLEIMFHVSTRMNSSSEDCMLQKTRHLGNDEIHIVWSEHWRDYRRGIIPTEFCDVLIVIYPLKNKLYRIQVIRKSEVPFFGPLYNEMIVDHGVLPGLIRATAVNASRAKRAMMTAYKTYFEDRAKALDSIIDKFKQPTTFEEFVANVFNPAQTGTIIQ---GNMRPGGMSSSTISSNTNRSDLSAVLLDPMSTHTGWSEEQQRPRAHTELGERTRSTLGEDRKILHEEHVPLRKKLSSGVPPGSHSNPGVGFSQVPLSDEEDMFNPPSGSAR-KFSTKSPVQAMSAVRRPMAVRQ 1830          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971383|ref|XP_021917097.1| (ral GTPase-activating protein subunit alpha-1 isoform X7 [Zootermopsis nevadensis])

HSP 1 Score: 1113.6 bits (2879), Expect = 0.000e+0
Identity = 594/1121 (52.99%), Postives = 762/1121 (67.98%), Query Frame = 0

Query:   77 YNFHLESKRRHRSVESMYTYSR------SESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA--HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1155
            Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A      P     +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:  704 YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMTTFP-----HQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1773          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971377|ref|XP_021917094.1| (ral GTPase-activating protein subunit alpha-1 isoform X4 [Zootermopsis nevadensis])

HSP 1 Score: 1113.21 bits (2878), Expect = 0.000e+0
Identity = 594/1121 (52.99%), Postives = 762/1121 (67.98%), Query Frame = 0

Query:   77 YNFHLESKRRHRSVESMYTY------SRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA--HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1155
            Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A      P     +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:  815 YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMTTFP-----HQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1884          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971371|ref|XP_021917090.1| (ral GTPase-activating protein subunit alpha-1 isoform X1 [Zootermopsis nevadensis])

HSP 1 Score: 1113.21 bits (2878), Expect = 0.000e+0
Identity = 594/1121 (52.99%), Postives = 762/1121 (67.98%), Query Frame = 0

Query:   77 YNFHLESKRRHRSVESMYTY------SRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA--HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1155
            Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A      P     +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:  821 YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMTTFP-----HQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1890          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971373|ref|XP_021917091.1| (ral GTPase-activating protein subunit alpha-1 isoform X2 [Zootermopsis nevadensis])

HSP 1 Score: 1112.83 bits (2877), Expect = 0.000e+0
Identity = 594/1121 (52.99%), Postives = 762/1121 (67.98%), Query Frame = 0

Query:   77 YNFHLESKRRHRSVESMYTY------SRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA--HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1155
            Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A      P     +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:  820 YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMTTFP-----HQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1889          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971379|ref|XP_021917095.1| (ral GTPase-activating protein subunit alpha-1 isoform X5 [Zootermopsis nevadensis] >gi|646718494|gb|KDR20934.1| 250 kDa substrate of Akt [Zootermopsis nevadensis])

HSP 1 Score: 1112.83 bits (2877), Expect = 0.000e+0
Identity = 594/1121 (52.99%), Postives = 762/1121 (67.98%), Query Frame = 0

Query:   77 YNFHLESKRRHRSVESMYTY------SRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA--HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1155
            Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A      P     +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:  812 YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMTTFP-----HQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1881          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971381|ref|XP_021917096.1| (ral GTPase-activating protein subunit alpha-1 isoform X6 [Zootermopsis nevadensis])

HSP 1 Score: 1112.44 bits (2876), Expect = 0.000e+0
Identity = 594/1121 (52.99%), Postives = 762/1121 (67.98%), Query Frame = 0

Query:   77 YNFHLESKRRHRSVESMYTY------SRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA--HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1155
            Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A      P     +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:  821 YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMTTFP-----HQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1890          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1227971375|ref|XP_021917093.1| (ral GTPase-activating protein subunit alpha-1 isoform X3 [Zootermopsis nevadensis])

HSP 1 Score: 1112.06 bits (2875), Expect = 0.000e+0
Identity = 594/1121 (52.99%), Postives = 762/1121 (67.98%), Query Frame = 0

Query:   77 YNFHLESKRRHRSVESMYTY------SRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIPHFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIP---------VRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSWDSCAM------------NGLTKSPNGIIEMDY-------SKLAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN-AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA--HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1155
            Y  H    R+ RS++S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + D  +H Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PPF I APWC KAL LP +YQ+GKL A RLLC +T  P D    + HLVQFYKVLH+GL G+   V+NTLI++TGPRFFSLMLPG++  + D + AAN  +S+S+L+G PRTEA S++GALL  P+   ++P+L PN+ E +++  +D KD I+ +LLK GK+EPAGLARCI+LSSLGI++Y EL H +F+PKIKEA+ VLL ALRFNNK + Q+ASD+LLLL DH    ++ YPE+P +I+EVLA T+  L P+    ++E +K LL SL+FCLGEWCMRIP   L     +G  LL  VF+ L              Q+RRG   SN S   G       +  P   V DFDPNI +D+ KE      T P+    S              +  HDS  P         V++AA+ V+ HL+NHL HFPM  GAA LSS+V E DD+PGL  DEL  +VF  PNIQLF+L+N+ L+SLVELP ++         ++T+  QVR+ILRD+SGK SWD+  +            +   ++ +G+            S+L + +L SS  G +  S+     +R R   +LPT +N AEDMDNLDDLLQY+ HTSPEC E +  P N   P       EVE D I+ V++QR  EQ+++++ + ++   A      P     +Q   S FQ CR LFS LGL+ WE+R ++HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+RIQVSRKPEVPYFGPLFNE IV  +VLPGL+RA+AI+ASR+KRSML FYQ++YEER+K+LE +I NHKD TTFEEF  QV+SP    + F
Sbjct:  818 YQSHNRLSRKSRSMDSLRGVRSGTPIQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLGGGDVTSLD---VPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLIDPALHNQVFEYLVELCDTLAKIRLNQGVSGDNQVTPPPPDLIPPFNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISSPRDHLVQFYKVLHQGLVGTDQAVINTLIQFTGPRFFSLMLPGHSLLLYDIIHAANTIVSSSDLRGTPRTEAMSILGALLCLPNTFAEMPVLQPNASEFTVMPCSDAKDHILSILLKCGKREPAGLARCIALSSLGIYLYHELTHETFYPKIKEAVNVLLLALRFNNKIIGQVASDILLLLCDHADRLIERYPEIPPRIIEVLALTISHLMPQSIVTMSERDKHLLTSLMFCLGEWCMRIPANCLLA-SYNGSCLLLTVFKVLN-------------QLRRGTSGSNDSGSIG------GLFQPD-FVQDFDPNILLDDLKE----VATPPVGRRSS--------------TMTHDSGTPSHHHHSSQSVQMAARMVMMHLINHLGHFPMGIGAARLSSMVVEHDDVPGLSADELSSEVFSAPNIQLFVLSNSLLVSLVELPALDVPGGGVTAGLRTAPSQVRVILRDLSGKASWDASILYCSPEDSVCSHRHAKEETEDGVRTAGLPRPISVSSRLEEAMLTSSFVGGELHSSPPQHTLRHRPPDILPTCDNGAEDMDNLDDLLQYLGHTSPECLESLDTPRNIPAPPPPPLTREVEEDTITMVLNQRNAEQEYLQRHSADIMMQAGCMTTFP-----HQSSHSPFQHCRLLFSQLGLAGWERRSHLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKNSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRIQVSRKPEVPYFGPLFNESIVDDKVLPGLVRASAISASRSKRSMLPFYQNYYEERAKSLETVIRNHKDATTFEEFTAQVYSPVQTVSPF 1887          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1339045761|ref|XP_023711023.1| (probable Rho GTPase-activating protein CG5521 isoform X1 [Cryptotermes secundus])

HSP 1 Score: 1097.42 bits (2837), Expect = 0.000e+0
Identity = 591/1110 (53.24%), Postives = 760/1110 (68.47%), Query Frame = 0

Query:   85 RRHRSVESMYTY------SRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIP-HFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSW----------DSCAMNGLTK---SPNGIIEMDYSK-------LAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN--AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA-----HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1155
            R+ RSV+S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + +  +H+Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PP  I APWC KAL LP +YQ+GKL A RLLC +T  P D  L + HL QFYKVLH+GL G+   V+NTL++++GPRFFSLMLPG++  + DF+ AAN  +S+S+L+G PRTEA S++GALL  P+   +  +L PN+ E +++  +D KD I+ +LLK GK+EPAG ARCI+LSSLGI++Y EL H  FHPK KEA+ VLL ALRFNNK V Q+ASD+LLLL DH    ++ YPE+P +I+EVLA TL  L P+     +E +K LL SL+FCLGEWCM+IP +++L     +G  LL  VF+ L Q                        +  G   +   +  P  +V DFDPNI +D+ KEG     ++P    +S +  QE+ S+         SN  +++AA+ V+ HLVNHL HFPM  GAA LSS+V E DD+PGL TDEL  +VF  PNIQLF+L+N+ ++SLVELP +E         ++T+  QVR+ILRD+SGK SW          DS   + LTK   + +G+     SK       L + +L SS  G +  S      +R R   +LPT +N AEDMDNLDDLLQY+ +TSPEC E +  P N  A P   L   E E D I+ V++QR  EQ+++++ + ++T  A      P  P Q        S FQ CR LFS LGL+ WE+R  +HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+R+QVSRK EVPYFGPLFNE IV  +VLPGL+RA+AI ASRAKRSML FYQ++YEER+K+LE ++ NHKD TTFEEF  QV+SP   T+ F
Sbjct:  823 RKSRSVDSLRGARSGTPNQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLSGGDGTSLD---LPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLTNPALHSQVFEYLVELCDTLAKIRLNQGVSDDNQVTPPPPDLIPPLNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISLPRDHLSQFYKVLHQGLVGTDQAVINTLVQFSGPRFFSLMLPGHSLLLYDFIHAANTIVSSSDLRGTPRTEAVSILGALLCLPNTFAETLVLQPNAGEFTMMPCSDAKDHILSILLKCGKREPAGQARCIALSSLGIYLYQELTHEIFHPKNKEAMNVLLLALRFNNKTVGQVASDILLLLCDHADRLVEHYPEIPSRIIEVLALTLSHLMPQNNVTTSERDKHLLTSLMFCLGEWCMKIPANYLLA--SNNGSCLLLTVFKVLNQL------------------QGGGGSGSGVSGSTGGLFQPD-LVQDFDPNILLDDLKEG-----STPPVGRRSSAMIQESGSLSHHHH----SNQSIQMAARMVMMHLVNHLGHFPMGIGAARLSSMVVEHDDVPGLATDELSAEVFSAPNIQLFVLSNSLMVSLVELPALEVPGGGITAGLRTAPSQVRIILRDLSGKASWDASILYCSPEDSICSHRLTKEEETEDGVGICGSSKPISVSSRLEEAMLTSSFVGGELHSCPPQHTLRHRPPHMLPTCDNGAEDMDNLDDLLQYLGYTSPECLESLDTPRNIPATPPPPL-TREAEEDTITMVLNQRNAEQEYLQRRSADITMLAGCMATFPHKPSQ--------SPFQHCRLLFSQLGLAGWERRSQLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKSSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRVQVSRKSEVPYFGPLFNESIVDDKVLPGLVRASAIGASRAKRSMLPFYQNYYEERAKSLETVVRNHKDATTFEEFTAQVYSPVQATSPF 1886          

BLAST of 250 kda substrate of akt vs. nr
Match: gi|1339045763|ref|XP_023711024.1| (probable Rho GTPase-activating protein CG5521 isoform X2 [Cryptotermes secundus])

HSP 1 Score: 1097.03 bits (2836), Expect = 0.000e+0
Identity = 591/1110 (53.24%), Postives = 760/1110 (68.47%), Query Frame = 0

Query:   85 RRHRSVESMYTY------SRSESDGVGSRSCSPAPSSGLECTSMKDSPMQLDHDTISEGGLSDQLSHHTIHSSTHHEPRSVMSGGAVKGWLPDVAVVLWRRMLGALGNINHIADTVIHAQIYKYLIELHEIMVKIRNNQGVSLDNNFTPPPPDFVPPFTIFAPWCCKALALPEAYQRGKLYALRLLCLLTCRPHDTPLSKTHLVQFYKVLHEGLTGSKLDVMNTLIKYTGPRFFSLMLPGYTAYILDFLFAANCTISTSELKGVPRTEATSVVGALLAFPSILEDIPLLLPNSHELSLISSADVKDQIIGVLLKSGKKEPAGLARCISLSSLGIFVYSELIHGSFHPKIKEAIQVLLTALRFNNKAVAQIASDMLLLLADHVKNFLDFYPEVPKKIVEVLAKTLISLTPRGEAGVNEDEKRLLLSLLFCLGEWCMRIP-HFILTQPQEDGKSLLYHVFEALQQACDPGPQGAPEIQVRRGNGHSNTSTREGFVTAAKAVLSPAVMVSDFDPNIHVDNTKEGYMSNNTSPLKSSKSRSGSQEALSIIQVRSPDHDSNIPVRLAAKTVLSHLVNHLFHFPMSNGAANLSSLVAECDDIPGLGTDELCMDVFQQPNIQLFILNNTTLMSLVELPLME--NVNSVQEIQTSSCQVRLILRDISGKFSW----------DSCAMNGLTK---SPNGIIEMDYSK-------LAQMVLRSS-TGSK--SNVSHQAMRRRNETVLPTQENAAEDMDNLDDLLQYISHTSPECKEEVGRPLN--AVPTTTLFENEVESDVISGVMSQRTLEQDHVKKLAPELTYSA-----HPEVPPQLEENQDYPSVFQQCRRLFSHLGLSSWEQRPNVHLMKKNERLLRELKNLDAKRCREAHKIAVVFVGEGQEDKQSILSNASGSQAYEEFVSGLAWEVELETHTGFMGGLQKSKTTGETAPYYATSFLEVMFHVATRMPSLSEESMLQKTRHIGNDEIHIVWSEHWRDYRRGILPTEFCDVLIVIYPLKNKLFRIQVSRKPEVPYFGPLFNEMIVGQRVLPGLIRATAINASRAKRSMLTFYQSHYEERSKALENIIANHKDKTTFEEFITQVFSPAPLTNLF 1155
            R+ RSV+S+           SES+G  +RS SPA SSG+E  S+KDSPMQ+D   +  GG    L    +      E RSVM+GG+V+GWLPDVAVVLWRRMLGALG++N + +  +H+Q+++YL+EL + + KIR NQGVS DN  TPPPPD +PP  I APWC KAL LP +YQ+GKL A RLLC +T  P D  L + HL QFYKVLH+GL G+   V+NTL++++GPRFFSLMLPG++  + DF+ AAN  +S+S+L+G PRTEA S++GALL  P+   +  +L PN+ E +++  +D KD I+ +LLK GK+EPAG ARCI+LSSLGI++Y EL H  FHPK KEA+ VLL ALRFNNK V Q+ASD+LLLL DH    ++ YPE+P +I+EVLA TL  L P+     +E +K LL SL+FCLGEWCM+IP +++L     +G  LL  VF+ L Q                        +  G   +   +  P  +V DFDPNI +D+ KEG     ++P    +S +  QE+ S+         SN  +++AA+ V+ HLVNHL HFPM  GAA LSS+V E DD+PGL TDEL  +VF  PNIQLF+L+N+ ++SLVELP +E         ++T+  QVR+ILRD+SGK SW          DS   + LTK   + +G+     SK       L + +L SS  G +  S      +R R   +LPT +N AEDMDNLDDLLQY+ +TSPEC E +  P N  A P   L   E E D I+ V++QR  EQ+++++ + ++T  A      P  P Q        S FQ CR LFS LGL+ WE+R  +HL+ KNE+LLREL+NLD +RCRE HKIAVV+V EGQEDK SILSN  GSQAYEEF++GLAWEVELE+HTGF+GGLQ++K+TGETAPYYATSFLEV+FHVATRMPS S+ES+LQKTRH+GNDE+HIVWSEH RDYRRGI+PTEFCDVLIVIYPL N+L+R+QVSRK EVPYFGPLFNE IV  +VLPGL+RA+AI ASRAKRSML FYQ++YEER+K+LE ++ NHKD TTFEEF  QV+SP   T+ F
Sbjct:  822 RKSRSVDSLRGARSGTPNQYSESEG-PTRSPSPAASSGVESNSIKDSPMQID---VLSGGDGTSLD---LPDGGSLERRSVMAGGSVRGWLPDVAVVLWRRMLGALGDVNQLTNPALHSQVFEYLVELCDTLAKIRLNQGVSDDNQVTPPPPDLIPPLNIIAPWCFKALTLPSSYQKGKLCAYRLLCTMTVTPQDISLPRDHLSQFYKVLHQGLVGTDQAVINTLVQFSGPRFFSLMLPGHSLLLYDFIHAANTIVSSSDLRGTPRTEAVSILGALLCLPNTFAETLVLQPNAGEFTMMPCSDAKDHILSILLKCGKREPAGQARCIALSSLGIYLYQELTHEIFHPKNKEAMNVLLLALRFNNKTVGQVASDILLLLCDHADRLVEHYPEIPSRIIEVLALTLSHLMPQNNVTTSERDKHLLTSLMFCLGEWCMKIPANYLLA--SNNGSCLLLTVFKVLNQL------------------QGGGGSGSGVSGSTGGLFQPD-LVQDFDPNILLDDLKEG-----STPPVGRRSSAMIQESGSLSHHHH----SNQSIQMAARMVMMHLVNHLGHFPMGIGAARLSSMVVEHDDVPGLATDELSAEVFSAPNIQLFVLSNSLMVSLVELPALEVPGGGITAGLRTAPSQVRIILRDLSGKASWDASILYCSPEDSICSHRLTKEEETEDGVGICGSSKPISVSSRLEEAMLTSSFVGGELHSCPPQHTLRHRPPHMLPTCDNGAEDMDNLDDLLQYLGYTSPECLESLDTPRNIPATPPPPL-TREAEEDTITMVLNQRNAEQEYLQRRSADITMLAGCMATFPHKPSQ--------SPFQHCRLLFSQLGLAGWERRSQLHLLSKNEKLLRELRNLDTQRCRETHKIAVVYVAEGQEDKSSILSNTCGSQAYEEFIAGLAWEVELESHTGFLGGLQRNKSTGETAPYYATSFLEVIFHVATRMPSSSQESLLQKTRHLGNDEVHIVWSEHSRDYRRGIIPTEFCDVLIVIYPLPNRLYRVQVSRKSEVPYFGPLFNESIVDDKVLPGLVRASAIGASRAKRSMLPFYQNYYEERAKSLETVVRNHKDATTFEEFTAQVYSPVQATSPF 1885