BLAST of hypothetical protein DAPPUDRAFT_318475 vs. L. salmonis genes
Match: EMLSAG00000008294 (supercontig:LSalAtl2s:LSalAtl2s501:4024:96935:1 gene:EMLSAG00000008294 transcript:EMLSAT00000008294 description:"maker-LSalAtl2s501-augustus-gene-1.9")

HSP 1 Score: 66.2402 bits (160), Expect = 3.678e-11
Identity = 76/310 (24.52%), Postives = 131/310 (42.26%), Query Frame = 0

Query:  517 FDSAVQRMTTVTKKKGSQQFDVYIKGAP----EKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANH--RWKEVDD---LSRSDLEQKAD-----------FLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPD----------QTIIRVEAEL--IPDGYSQGLNVSYMLEENEKSNIVHDSNFIKSVQ-------------EKNYVFACDGKTFALIRNHDKALLDRIVQ-RGKIFARMLPEQKIHLIECMKDLGRQVIMC-GDGCNDCGALKTAHAGISL 779
            F++  +RM+ + K+  + +  +Y KGA     +++AG    E +       L  +  +GLR +   +K L  +    WK       +S  D E+K D            LG   +++ ++D     I  L+ A I   ++TGD   TAI++G  C+L++ D          QT   V+ +L  I +     +N S    E ++   + D N   S+              E+N V+  D        + +K+ +  +      I  R+ P QK  ++E +K   + V +  GDG ND   +KTAH G  L
Sbjct:  151 FNNVRKRMSVILKR--NDKIRLYCKGADTVILQRLAG--DQEDLKSKTQDHLDKFASEGLRTLVVGYKDLTESQFEDWKHAHHEAAISLEDREEKLDNVYDQIENNLKLLGATAIEDKLQDGVAQTISNLNLAGIKIWVLTGDKQETAINIGYSCQLLRDDLYEEPYIIDGQTFEAVQTQLLQIRNNIMSSMNGSVNGAE-KQHEFIKDGNHRDSISMLTFSDSSFFSDDERNNVYY-DKPRPHFPHDGNKSPISVVFNPEAVICCRVTPLQKAQVVELVKKYKKAVTLAIGDGANDVSMIKTAHIGTLL 454          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. L. salmonis genes
Match: EMLSAG00000002213 (supercontig:LSalAtl2s:LSalAtl2s141:398215:401318:1 gene:EMLSAG00000002213 transcript:EMLSAT00000002213 description:"augustus_masked-LSalAtl2s141-processed-gene-4.6")

HSP 1 Score: 65.4698 bits (158), Expect = 8.731e-11
Identity = 126/611 (20.62%), Postives = 243/611 (39.77%), Query Frame = 0

Query:  220 VNESMLTGESIPITKISIPDENNVQFAYD--LQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGEL------VRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSL-YMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGV---CEVKNANFQETQADPTQLALDSR-----LVQAMATCH---------SLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNI-KTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYI-KGAPEKIAGTCKP-----ETIPPDFASTLQWYTKQ----------GLRVIAAAHKSLNANHR----WKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSNIVHDSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGK--IFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSM 781
            V+ S LTGES P ++       + +F ++  L+ +++ F  T  ++G A      K +VI+TG  T  G +      +     P   +       +  + VFL +    +A  L Y W+       + ++  + I+   VP  L A +T       KR+        + + +   G    +  DKTGTLT+  + ++ +     +  A+  E QA    L  ++      L + +A C+          L+ ++ E+ G+  +  L + +E                                +GN+ K    N +++ +   PF+S  +   ++ +   +++ +V + KGAPE I   C       E +P D     +W  K           G RV+     +L           + D+++ +   +   FLGLI + +  +     A+++   A I  +MVTGD+ +TA ++ +   ++  D   +   AE         + +  +     ++ ++H +       E+                     LD I+   K  +FAR  P+QK+ ++E  + +G  V + GDG ND  ALK A  G+++ +
Sbjct:  196 VDNSSLTGESEPQSR-------SREFTHENPLETKNIAFFSTHAVEGTA------KGIVIKTGDSTVMGRIAGLASGLERGETPISREISHFIHLITGVAVFLGVIFFIIALILGYHWV-------DAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMASKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVSHMWFDSIIVVADTSENQAG--DLGFENMPSWKYLERCVALCNRAEFKPGQEELLIIEKEVIGDASEAALLKCMELT------------------------------TGNVMKYRAKNKKVSEI---PFNSTNKYQLSIHE---TEEGNVLVMKGAPEIILERCSSILNHKENLPLD----QEWKDKYHQAYIKLGGLGERVLGFCDLNLPLEKYPIGYCFDTDEINFTT--ENLRFLGLISLIDPPRATVPSAVEKCRTAGIKVIMVTGDHPITAKAIAKAVGIISEDTKTVEDIAE------ENNIPIEEVDSREAEAFVMHGNELKDKTDEE---------------------LDEILINHKEIVFARTSPQQKLIIVEGCQRVGSIVAVTGDGVNDSPALKKADIGVAMDI 715          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. L. salmonis genes
Match: EMLSAG00000007588 (supercontig:LSalAtl2s:LSalAtl2s438:539356:544479:1 gene:EMLSAG00000007588 transcript:EMLSAT00000007588 description:"maker-LSalAtl2s438-augustus-gene-5.3")

HSP 1 Score: 60.077 bits (144), Expect = 3.586e-9
Identity = 80/378 (21.16%), Postives = 156/378 (41.27%), Query Frame = 0

Query:  503 APSN--LEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNAN-----------------HRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSNIVHDSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQ-RGKIFARMLPEQK---IHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAE---ASVAAPFT-SRNVHIGCVPYLIREGRATM--VSAFASFKFGVAFCFTQLIAVL-MVFYIGTEPSDNQYLVV 850
            +P+N  +   IL  +PF S  +RM  + K  G+++   Y+KGA   ++   +      +    +    ++GLR +  A K+L+A+                 +R  +V  +  S LE+  + L +  +++ ++D     ++ L  A +   M+TGD + TA  + +   LV  +Q                   V    +   ++   H+ N  +  Q+   V   D     L   ++   +D        +  R  P+QK   +HLI+  +   ++    GDG ND   +++A  G+ +   E   AS+A  F+ ++  H+  +  L+  GR +    +A + F        T + A+   VFY  +      +L+V
Sbjct:  548 SPNNDIISYTILYIFPFTSESKRMGIILKDDGTEEITFYVKGADIVMSSIVQYNDWLEEEXDNM---AREGLRTLVVAKKTLSADQFQEFEQRYAAAKLSVENRSAQVTTVIES-LERDMELLCVTGVEDKLQDNVRTTLELLRNAGMKIWMLTGDKLETASCIAKSSCLVSKNQ------------------GVHIFKDVVNRAEAHHEMNAFRRRQDTTLVIKGDSLEVCL-EFYEHEFMDLATACPAVVCCRCSPQQKAQVVHLIQ--QHTNKRAAAIGDGGNDVSMIQSASVGVGIVGKEGKQASLAXXFSITQFAHVARL--LLVHGRNSYKRSAALSQFVIHRGLIITTMQAIFSAVFYFSSVSLYQGFLMV 898          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. L. salmonis genes
Match: EMLSAG00000012805 (supercontig:LSalAtl2s:LSalAtl2s975:43541:51448:1 gene:EMLSAG00000012805 transcript:EMLSAT00000012805 description:"augustus_masked-LSalAtl2s975-processed-gene-0.3")

HSP 1 Score: 56.6102 bits (135), Expect = 4.361e-8
Identity = 94/447 (21.03%), Postives = 171/447 (38.26%), Query Frame = 0

Query:  386 GGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALD---------SRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEI--AILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCK-------PETIPPDFAST---LQWYTKQGLRVIAAAHKSLNAN-----------------HRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSNIVHDSNFIKSVQEKNYVFACDGKT----FALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIM--CGDGCNDCGALKTAHAGISLSMAEASVAA 788
            G ++ +  DKTGTLTE N+ L   C +    F ++    + L+L+         +  +Q++A CH++     E++ N  D+             H    +PD   A   +      +  G+ +     S  EI   ++K  P +   +RM  +      +   V ++     I   CK       P  I  +   T   +  Y   GLR +    K L  +                 +R +++ D+ R ++E+    LG + +++ ++D     +  L +A I   ++TGD   TA+++ + C    P    +    +L  +G             ++ S+ + D  +   V +       DGK     FA     +  L   +  R  I  RM P QK  +++ +K+     I    GDG ND   ++ AH G  +   E   A 
Sbjct:  293 GQVEYLFSDKTGTLTE-NVMLFKECSIDGLRFADSAT--SLLSLNETKSSEKSINHFLQSLALCHTV-----EVSINDSDL-------------HYEASSPD-EKALVDICRKYGTAFMGTFDTGDEQSEEEILQLVIKDRPKEQLFKRMRILEFDSDRKCMSVIVQDEEGNIRLICKGAESSVLPNCISGEIEITKEHVDIYGTVGLRTLVVCEKQLTESELDQYLVKLKEASISLVNRSEKLKDVYR-EIEKDLHVLGAVAIEDKLQDGVKETLIRLGQAGIKIWILTGDKRETALNISQSCGHYXPSTMHL---LDLCKEG-------------SQVSDAITDYLYKTRVGDDRNCLVIDGKAVRSLFAFKDTIEPFLNLALRCRSVICCRMSPLQKAEIVKLIKNSPESPITXSIGDGGNDVSMIQEAHVGFGIMGKEGRAAV 700          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. SwissProt
Match: gi|223590262|sp|Q9H7F0.4|AT133_HUMAN (RecName: Full=Probable cation-transporting ATPase 13A3; AltName: Full=ATPase family homolog up-regulated in senescence cells 1)

HSP 1 Score: 663.3 bits (1710), Expect = 0.000e+0
Identity = 405/1210 (33.47%), Postives = 638/1210 (52.73%), Query Frame = 0

Query:   14 EEALVNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLK--DKFE-------QIFEEDVVDHSSPE-MTN---NSSPIC------------------------SFFINKKIKYVWDSGARTYVKLKNIDE-------FMKQAD--------FHKCKEGLGQLTV-----------------------------TDRHQEYGANLIKISVPPVL---------YLVFH-----------------EAKTELSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDEN-------NVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADP-TQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKG-------SRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSN------------IVHDSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLF----FQTLTYVGVWFYVQIQSWFVPYKFE--------AGLWPPN-------------PSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVLFAK-ILPW-YKLRIRGPHLPFEHLEEELRSKSGWPP 1062
            E   +N+GQ DEM + G+  +  K  I  L V+ +GG  L+LL W  + ++     +  +K+ + +LL+  D+F+       ++   +    SSP+ M+N   N   +C                         +F +  +KY W+     +  LK +DE       + K +         + K   G+ ++ V                             TD +  Y   ++ +S+  ++         Y++ H                 E   E+ S  LVPGDV+++P NG +M  CDA+L+ GTC+VNESMLTGES+P+TK ++P+ +       +  +  +  ++H +FCGT ++Q +   G  +KA+V+RTGF T+KG+LVR+IL+P P DF+ + D    +   + +  IG  Y++   I N   +   ++ SLDI+T  VPP LPAA+TA   +AQ+RL+K+GIFC+  + I +CG +++V FDKTGTLTE  LDL G+  V+NA F   + +   ++ + S+ V  MATCHSL K++G L+G+PLD+K+FEAI W + ++        +    PT+V PPK        + N    +   P+  EI I++ +PF SA+QRM+ V +  G ++ D Y+KGAPE IAG CKPET+P DF + L+ +TKQG RVIA AH+ L +   W +V ++SR  +E   DF+GLIIMQN +K ET   +++LH+A+I TVMVTGD++LTA+SV RDC ++ P   +I  EA    DG    +N  Y     + S+            +VHDS  ++ +Q   Y FA +GK+F++I  H + L+ +++  G +FARM P+QK  LIE ++++   V MCGDG NDCGALK AH GISLS  EASVA+PFTS+   I CVP LIREGRA ++++F  FKF   +   Q  +V +++ I +   D Q+L +D+ +  + +  +        LV Q+P   L+S   LFSV+S +     FQ+L +    F+V+ Q W+  +  +        +G W  +              +YE T +F +S    ++ AI FSKG+P+R+P + N       +       F+ L+        +     P  ++R+ ++ +++ N       E   +D   + K  LPW    R + P   + +L +EL     WPP
Sbjct:    5 ERKTINQGQEDEMEIYGYNLSRWKLAIVSLGVICSGGFLLLLLYWMPEWRVKATCVRAAIKDCEVVLLRTTDEFKMWFCAKIRVLSLETYPVSSPKSMSNKLSNGHAVCLIENPTEENRHRISKYSQTESQQIRYFTHHSVKYFWNDTIHNFDFLKGLDEGVSCTSIYEKHSAGLTKGMHAYRKLLYGVNEIAVKVPSVFKLLIKEVLNPFYIFQLFSVILWSTDEYYYYALAIVVMSIVSIVSSLYSIRKQYVMLHDMVATHSTVRVSVCRVNEEIEEIFSTDLVPGDVMVIPLNGTIM-PCDAVLINGTCIVNESMLTGESVPVTKTNLPNPSVDVKGIGDELYNPETHKRHTLFCGTTVIQTRFYTGELVKAIVVRTGFSTSKGQLVRSILYPKPTDFKLYRDAYLFLLCLVAVAGIGFIYTIINSILNEVQVGVIIIESLDIITITVPPALPAAMTAGIVYAQRRLKKIGIFCISPQRINICGQLNLVCFDKTGTLTEDGLDLWGIQRVENARFLSPEENVCNEMLVKSQFVACMATCHSLTKIEGVLSGDPLDLKMFEAIGW-ILEEATEEETALHNRIMPTVVRPPKQLLPESTPAGNQEMELFELPATYEIGIVRQFPFSSALQRMSVVARVLGDRKMDAYMKGAPEAIAGLCKPETVPVDFQNVLEDFTKQGFRVIALAHRKLESKLTWHKVQNISRDAIENNMDFMGLIIMQNKLKQETPAVLEDLHKANIRTVMVTGDSMLTAVSVARDCGMILPQDKVIIAEALPPKDGKVAKINWHYADSLTQCSHPSAIDPEAIPVKLVHDS--LEDLQMTRYHFAMNGKSFSVILEHFQDLVPKLMLHGTVFARMAPDQKTQLIEALQNVDYFVGMCGDGANDCGALKRAHGGISLSELEASVASPFTSKTPSISCVPNLIREGRAALITSFCVFKFMALYSIIQYFSVTLLYSILSNLGDFQFLFIDLAIILVVVFTMSLNPAWKELVAQRPPSGLISGALLFSVLSQIIICIGFQSLGF----FWVKQQPWYEVWHPKSDACNTTGSGFWNSSHVDNETELDEHNIQNYENTTVFFISSFQYLIVAIAFSKGKPFRQPCYKNYFFVFSVIFLYIFILFIMLYPVASVDQVLQIVCVP-YQWRVTMLIIVLVNAFVSITVEES-VDR--WGKCCLPWALGCRKKTPKAKYMYLAQELLVDPEWPP 1202          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. SwissProt
Match: gi|81910337|sp|Q5XF89.1|AT133_MOUSE (RecName: Full=Probable cation-transporting ATPase 13A3)

HSP 1 Score: 644.04 bits (1660), Expect = 0.000e+0
Identity = 406/1214 (33.44%), Postives = 617/1214 (50.82%), Query Frame = 0

Query:   14 EEALVNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLK--DKFEQIFEEDV----------------------------VDHSSPEMTNNS---SPICSFFINKKIKYVWDSGARTYVKLKNIDEFMKQAD-FHKCKEGLGQ-------------------------------------------LTVTDRHQEYGANLIKISVPPVL---------YLVFH-----------------EAKTELSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDEN-------NVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADP-TQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAP-------PKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSY---MLEENEKSNI---------VHDSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLF----FQTLTYVGVWFY-VQIQSWFVPYKFEAGLWPPN-------------PSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVL---FAKI-------------LPW-YKLRIRGPHLPFEHLEEELRSKSGWPP 1062
            E   +NKGQ DEM + G+     K  +  + V+ TGG  L+LL W  + ++     +  +K+ + +LL+  D+F   F   +                             + +  EM   S   S    +F +  I+Y W+     +  LK +DE +  A  + K   GL Q                                           L   D +  Y   ++ +SV  ++         Y++ H                 E   E+ S  LVPGDV+++P NG VM  CDA+L+ GTC+VNESMLTGES+P+TK ++P+ +         Q++ +  ++H +FCGT ++Q +   G  +KA+V+RTGF T+KG+LVR+IL+P P DF+ + D    +   + +  IG  Y++   I N   +QE ++ SLDI+T  VPP LPAA+TA   +AQ+RL+K+GIFC+  + I +CG +++V FDKTGTLTE  LDL G+  V+N  F   + +  +++ + S+ V  MATCHSL K++G L+G+PLD+K+FEAI W + ++        +    PT+V P       P  + N    +   P+  EI I++ +PF SA+QRM+ V +  G ++ D Y+KGAPE +A  CKPET+P DF   L+ YTKQG RVIA AH+ L +   W +V  +SR  +E   DF+GLIIMQN +K ET   +++LH+A+I TVMVTGDN+LTA+SV RDC ++ P   +I  EA    DG    +N  Y   + + +E S I          HDS  ++ ++   Y FA +GK+F++I  H + L+ +++  G +FARM P+QK  L+E ++++   V MCGDG NDCGALK AH GISLS  EASVA+PFTS+   I CVP LIREGRA ++++F  FKF   +   Q  +V +++ I +   D Q+L +D+ +  + +  +        LV Q+P   L+S   LFSV+S +     FQ+L +  V  Y V   +  V     +  W  +              +YE T +F +S    +  A+ FSKG+P+R+P + N    +  +       F+ L         +     P                   Y W +Y++  VL   F  I             L W    R + P   + +L +ELR    WPP
Sbjct:    5 ERKTINKGQEDEMEIHGYNLCRWKLAMVFVGVICTGGFLLLLLYWLPEWRVKATCVRAAVKDCEVVLLRTTDEFRVWFCAKIHFLPVENQPNLNAKCLVNEVSNGHAVHLTEENRCEMNKYSQSQSQQMRYFTHHSIRYFWNDAIHNFDFLKGLDEGVSCASLYEKHSAGLTQGMHAYRKLIYGVNEIAVKVPSVFKLLIKEVLNPFYIFQLFSVILWSVDEYYYYALAIVIMSVVSIISSLYSIRKQYVMLHDMVATHSTVRVSVCRENEEIEEIFSTDLVPGDVMIIPLNGTVM-PCDAVLINGTCIVNESMLTGESVPVTKTNLPNPSVDVKGMGEEQYSPETHKRHTLFCGTTVIQTRFYTGELVKAIVVRTGFSTSKGQLVRSILYPKPTDFKLYRDAYLFLLCLVVVAGIGFIYTIINSILNEKEVQEIIIKSLDIITITVPPALPAAMTAGIVYAQRRLKKVGIFCISPQRINICGQLNLVCFDKTGTLTEDGLDLWGIQRVENTRFLLPEDNVCSEMLVKSQFVACMATCHSLTKIEGVLSGDPLDLKMFEAIGW-ILEEATEEETALHNRIMPTVVRPSKQLLPEPTTAGNQEMELFELPAIYEIGIVRQFPFSSALQRMSVVARTLGEKRMDAYMKGAPEVVASLCKPETVPVDFEKVLEDYTKQGFRVIALAHRKLESKLTWHKVQHISRDAIENNMDFMGLIIMQNKLKQETPAVLEDLHKANIRTVMVTGDNMLTAVSVARDCGMILPQDKVIIAEALPPKDGKVAKINWHYTDSLSQCSESSAIDSEAIPIKLAHDS--LEDLEVTRYHFAMNGKSFSVILEHFQDLVPKLMLHGTVFARMAPDQKTQLVEALQNVDYFVGMCGDGANDCGALKRAHGGISLSELEASVASPFTSKTPSISCVPNLIREGRAALMTSFCVFKFMALYSIIQYFSVTLLYSILSNLGDFQFLFIDLAIILVVVFTMSLNPAWKELVAQRPPSGLISGALLFSVLSQIVISVGFQSLGFFWVKQYKVCDPNSDVCNTTRSACWNSSHLYNGTELDSCKIQNYENTTVFFISSFQYLTVAVAFSKGKPFRQPCYKNYFFVISVIILYVFILFIMLHPVASVDQVLEIMCVP-------------------YQWRIYMLIIVLINAFVSITVEESVDRWGKCCLSWALSCRKKTPKAKYMYLAQELRFDPEWPP 1195          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. SwissProt
Match: gi|74753861|sp|Q4VNC0.1|AT135_HUMAN (RecName: Full=Probable cation-transporting ATPase 13A5; AltName: Full=P5-ATPase isoform 5)

HSP 1 Score: 586.26 bits (1510), Expect = 0.000e+0
Identity = 356/934 (38.12%), Postives = 532/934 (56.96%), Query Frame = 0

Query:  185 ELSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQ----FAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALD-SRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAP-PKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEA----ELIPDGYSQGLNVSYMLEENEKSN------IVHDSNFIKSVQEKN--YVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVP-YKFEAGL----------------WPPNP--------SYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFN-CIFCYLWEMYLIDGVLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWPPI------GDCKN 1068
            EL S  LVPGD+++LP  G   + CDA+L++G+CVVNE MLTGESIP+TK  +P   N       + +  R+HV+FCGTE++Q K      ++AVV++TG+ T KG+LVR+IL+P PL+F+ +SD  K I     LG++G  Y+L +++ +G   ++ +  +L +LT  VPP+LPAALT  N +AQKRL+K  IFC+  + I +CG I++V FDKTGTLTE  LDL G     +  FQE  +  +  A+  S L  AMA+CHSLI L G + G+PLD+K+FE   W+++D   +  +  +G +   ++ P PK S++    I T         L  +PF S++QRM+ + +  G   F VY+KGAPE +A  C+ ET+P +F   L+ YT QG RVIA AHK+L   +   EV+ L+R  +E +  FLGL+IM+N +K ET   +KEL EA I TVM+TGDN+ TAI+V ++ E++ P   +I VEA    E +P       +V++ L EN+++        +H  N      E    Y FA  GK++ +I  H  +LL +I+  G +FARM P QK  LIE  + L   V MCGDG NDCGALK AHAGISLS  EASVA+PFTS+  +I CVP+LIREGRA +VS+F  FK+   +   Q I+ L++++      + QYL+ D+ +  +  + + +   +  L   +P   LLS   L S+     F  +  +  + YV+ Q W+   Y++                    W  N         S+E T ++ ++    +  A IFSKG+P+RKP++TN I +   +AA+  T F+ LF      +R    IP    +R++I+ V +   C+  ++ +  L +  L+  I   +    +     +   +++L   S WPPI      GD KN
Sbjct:  275 ELESRLLVPGDILILP--GKFSLPCDAVLIDGSCVVNEGMLTGESIPVTKTPLPQMENTMPWKCHSLEDYRKHVLFCGTEVIQVKPSGQGPVRAVVLQTGYNTAKGDLVRSILYPRPLNFKLYSDAFKFIVFLACLGVMGFFYALGVYMYHGVPPKDTVTMALILLTVTVPPVLPAALTIGNVYAQKRLKKKKIFCISPQRINMCGQINLVCFDKTGTLTEDGLDLWGTVPTADNCFQEAHSFASGQAVPWSPLCAAMASCHSLILLNGTIQGDPLDLKMFEGTAWKMED--CIVDSCKFGTSVSNIIKPGPKASKSPVEAIIT---------LCQFPFSSSLQRMSVIAQLAGENHFHVYMKGAPEMVARFCRSETVPKNFPQELRSYTVQGFRVIALAHKTLKMGNL-SEVEHLAREKVESELTFLGLLIMENRLKKETKLVLKELSEARIRTVMITGDNLQTAITVAKNSEMIPPGSQVIIVEADEPEEFVPA------SVTWQLVENQETGPGKKEIYMHTGNSSTPRGEGGSCYHFAMSGKSYQVIFQHFNSLLPKILVNGTVFARMSPGQKSSLIEEFQKLNYYVGMCGDGANDCGALKAAHAGISLSEQEASVASPFTSKTTNIQCVPHLIREGRAALVSSFGVFKYLTMYGIIQFISALLLYWQLQLFGNYQYLMQDVAITLMVCLTMSSTHAYPKLAPYRPAGQLLSPPLLLSIFLNSCFSCIVQISAFLYVKQQPWYCEVYQYSECFLANQSNFSTNVSLERNWTGNATLIPGSILSFETTTLWPITTINYITVAFIFSKGKPFRKPIYTNYIFSFLLLAALGLTIFI-LFSDFQVIYRGMELIPTITSWRVLILVVALTQFCVAFFVEDSILQNHELWLLIKREFGFYSKS---QYRTWQKKLAEDSTWPPINRTDYSGDGKN 1184          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. SwissProt
Match: gi|296439435|sp|Q4VNC1.3|AT134_HUMAN (RecName: Full=Probable cation-transporting ATPase 13A4; AltName: Full=P5-ATPase isoform 4)

HSP 1 Score: 584.719 bits (1506), Expect = 0.000e+0
Identity = 352/930 (37.85%), Postives = 522/930 (56.13%), Query Frame = 0

Query:  185 ELSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENN-----VQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALD-SRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWEL----QDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSNIVH--DSNFIK-------SVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWF-----------------------VPYKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDG-VLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWPPIGDCKNNEI 1071
            EL S  LVPGD+++L T   V+M CDA+L+EG+CVV+E MLTGESIP+TK  +P  ++      Q   D +R HV+FCGTE++Q KA     ++AVV++TGF T KG+LVR+IL+P P++FQ + D ++ +   +    IGM Y+L +++ +G   +E +  +LD++T  VPP LPAALT    +AQ+RL+K GIFC+  + I +CG +++V FDKTGTLT   LDL GV       FQE  +  +  AL    L  AMA+CHSLI L G + G+PLD+K+FEA  WE+     D H  GV P + M         K  R  S      P    IAIL  +PF SA+QRMT + ++ G  +   ++KGAPE++A  C+PET+P  F S LQ YT QG RVIA A+K L  +H       L+R  +E    FLGL+I++N +K+ET   ++EL  A I TVM+TGDN+ TAI+V R   +V   Q +I +EA       S   ++S+ L E EK +I++    N+I          +E +Y FA  GK+F +I  H  +LL +I+  G IFARM P QK  L+E  + L   V MCGDG NDCGALK AH GISLS  EASVA+PFTS+  +I CVP+LI+EGRA +V++F  FK+   +   Q + VL++++     S+ Q+L  D+ +  L  + +   G +  LV  +P   L+S   L SVI  +      ++  +  VQ Q W+                        P K E+       S+E T ++ L     +  A++FSKG+P+R+P +TN I  +  +  +    F+   +  +   R++    P L +R  IV ++  N I   + E  +I+   L+  I   +  + +     +   + +L +   WPP+    ++++
Sbjct:  274 ELESRVLVPGDLLIL-TGNKVLMPCDAVLIEGSCVVDEGMLTGESIPVTKTPLPKMDSSVPWKTQSEADYKR-HVLFCGTEVIQAKAACSGTVRAVVLQTGFNTAKGDLVRSILYPKPVNFQLYRDAIRFLLCLVGTATIGMIYTLCVYVLSGEPPEEVVRKALDVITIAVPPALPAALTTGIIYAQRRLKKRGIFCISPQRINVCGQLNLVCFDKTGTLTRDGLDLWGVVSCDRNGFQEVHSFASGQALPWGPLCAAMASCHSLILLDGTIQGDPLDLKMFEATTWEMAFSGDDFHIKGV-PAHAMVV-------KPCRTAS----QVPVE-GIAILHQFPFSSALQRMTVIVQEMGGDRL-AFMKGAPERVASFCQPETVPTSFVSELQIYTTQGFRVIALAYKKLENDH---HATTLTRETVESDLIFLGLLILENRLKEETKPVLEELISARIRTVMITGDNLQTAITVARKSGMVSESQKVILIEANETTG--SSSASISWTLVE-EKKHIMYGNQDNYINIRDEVSDKGREGSYHFALTGKSFHVISQHFSSLLPKILINGTIFARMSPGQKSSLVEEFQKLDYFVGMCGDGANDCGALKMAHVGISLSEQEASVASPFTSKTPNIECVPHLIKEGRAALVTSFCMFKYMALYSMIQYVGVLLLYWETNSLSNYQFLFQDLAITTLIGVTMNLNGAYPKLVPFRPAGRLISPPLLLSVIFNILLSLAMHIAGFILVQRQPWYSVEIHSACTVQNESISELTMSPTAPEKMESN--STFTSFENTTVWFLGTINCITVALVFSKGKPFRQPTYTNYIFVLVLIIQLGVCLFILFADIPELYRRLDLLCTPVL-WRASIVIMLSLNFIVSLVAEEAVIENRALWMMIKRCFGYQSKS---QYRIWQRDLANDPSWPPLNQTSHSDM 1175          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. SwissProt
Match: gi|81862737|sp|Q5XF90.1|AT134_MOUSE (RecName: Full=Probable cation-transporting ATPase 13A4; AltName: Full=P5-ATPase isoform 4)

HSP 1 Score: 572.007 bits (1473), Expect = 0.000e+0
Identity = 385/1210 (31.82%), Postives = 618/1210 (51.07%), Query Frame = 0

Query:    3 GSHQSKAITMSEEALVNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKL--NCMYSKVELKEAQKILLK--DKFEQIFEEDVVDHSSPEMTNNSS-----PICS---FFINK---------------KIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLGQ---------------------------------------------LTVTDRHQEYGANLIKISVPPVLYLVF-----------------------HEAKT---ELSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQFAYDLQ-----RQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLAL-DSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWEL----QDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSNIVHDSNFIKSVQE--------KNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWF-----------------------VPYKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDG-VLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWPPIGDCKNNEIK 1072
            G H  K    S+ AL+N+G  +EM + G+     +  + ++  + + G+  ++  WR   ++  NC+     L+EA  +LLK  D+F+    + V+  S   +++ S      P+ +   + IN+               KI+YVW++    + K+ ++++++  A  H+ K GLG                                              L  ++ ++EY   +I +SV  +   V+                       +E K    +L S  LVPGD+++L T   V M CDAIL++G+CVV+E MLTGESIP+TK  +  +      + +Q     R+HV+FCGTE++Q KA     ++AVV++TGF T KG+LVR+IL+P P++F+ + D ++ +   +    IGM Y+L +++ +G   +E +  +LD++T  VPP LPAALT    +AQ+RL+K GIFC+  + I +CG +++V FDKTGTLT G LD  GV       FQ   +  +  AL    L  AMA+CHSLI L G + G+PLD+K+FEA +WE+     D H         M   T+V  P      +  +   P+   +AI+  +PF SA+QRMT + ++ G  +   ++KGAPE++A  C+P+T+P  F S LQ YT QG RVIA A+K L  +        L R  +E    FLGL+I++N +K+ET   ++EL  A I TVM+TGDN+ TAI+V R   +V   Q +I VEA       S   ++S+ L E +K       +   +++E        ++Y FA  GK+F +I  +  +LL +I+  G IFARM P QK  L+E  + L   V MCGDG NDCGALK AH GISLS  EASVA+PFTS+  +I CVP+LI+EGRA +V++F  FK+   +   Q + VL++++     S+ Q+L  D+ +  L  + +   G +  LV  +P   L+S   L SV+  +      ++  +  VQ Q W+                       VP K  +       S+E T I+ L     +  A++FSKG+P+R+P +TN I  +  +  +    F+   +  +   R++    P L +R+ I+ ++  N +     E  +I+   L+  +   +  + +     +   +  L + S WPP+     ++++
Sbjct:    2 GDHLEK----SQHALLNEGDENEMEIFGYRTQGCRKALCLIGSIFSLGMLPLVFYWRPAWRVWANCV--PCSLQEADVVLLKTTDEFKIYSWKKVIWISLSALSSTSGLTPDHPLITDEGYIINRAIRKPDLKVRYIKVQKIRYVWNNLEGQFQKIGSLEDWLSSAKIHQ-KFGLGLTSEEQEIRRLICGPNAIDVEITPIWKLLIKEVLNPFYIFQLFSVCLWFSEDYKEYALAIILMSVISIALTVYDLRQQSVKLHHLVESHNSITVSVYERKAGAQDLESRLLVPGDLLIL-TGSRVQMPCDAILIDGSCVVDEGMLTGESIPVTKTPL-SQTASSVPWKMQSEADPRRHVLFCGTEVIQAKAAGSGAVRAVVLQTGFNTAKGDLVRSILYPKPMNFKLYRDAIRFLLCLVGTATIGMVYTLCVYVLSGEPPEEVVRKALDVITIAVPPALPAALTTGIIYAQRRLKKKGIFCISPQRINVCGQLNLVCFDKTGTLTRGGLDPWGVVPCDQNGFQAVHSFASGKALPQGPLCAAMASCHSLILLDGTIQGDPLDLKMFEATKWEMTASGDDLHIK------EMLAHTIVVKP------TDMVGQVPAE-GLAIVHQFPFSSALQRMTVIVQEMGGGRL-AFMKGAPERVASFCQPDTVPTSFISELQIYTTQGFRVIALAYKKLEMDC---PTTALMREKVESDLVFLGLLILENRLKEETKPVLEELISARIRTVMITGDNLQTAITVARKSGMVSEGQKVILVEANEATG--SSSASISWKLVEEKKPGPFGSQDTYINIREEVPENGRDRSYHFALSGKSFHVISQYFSSLLPKILINGTIFARMSPGQKSSLVEEFQKLDYFVGMCGDGANDCGALKMAHVGISLSEQEASVASPFTSKTPNIECVPHLIKEGRAALVTSFCMFKYMALYSMIQYVGVLLLYWKTNSLSNYQFLFQDLAITTLIGVTMNLNGANPKLVPFRPAGRLISPPLLLSVVLNILLSLAMHIVGFILVQKQPWYIMDYHSVCPVRNESASALAASPSVPEKTRSN--STFASFENTTIWFLGTINCIFVALVFSKGKPFRQPTYTNYIFVLVLILQMGVCLFILFADIPEMHRRLDLLCTPVL-WRVYILIMISSNFVVSLAVEKAIIENRALWIAVKRCFGYQSKS---QYRIWQRNLANDSSWPPLNQTSYSDMQ 1177          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. SwissProt
Match: gi|82082056|sp|Q5ZKB7.1|AT134_CHICK (RecName: Full=Probable cation-transporting ATPase 13A4; AltName: Full=P5-ATPase isoform 4)

HSP 1 Score: 571.622 bits (1472), Expect = 0.000e+0
Identity = 328/926 (35.42%), Postives = 506/926 (54.64%), Query Frame = 0

Query:  181 EAKTELSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQ----FAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALD-SRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSNIVHDS------NFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAGLWPPN-----------------------------PSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVLFAKILPWYKLRIR-GPHLPFEHLEEELRSKSGWPPIGD 1065
            E   EL S  LVPGD+++L   G  ++ CDAIL+ G C+VNESMLTGESIP+TK  +P  +N++       +  ++HV+FCGTE++Q K      +KAVV++TGF T KG+LVR+IL+P P++F+ + D L+ +   +    IGM Y++ ++  NG    E +  +LD++T  VPP LPAALT    + Q+RL+K GIFC+  + I +CG ++++ FDKTGTLTE  LDL G+   +   FQ+ +  P   +L      +AM  CHSLI L+G++ G+PLDVK+FEA  W + D     +          +   PK +        TA  +  I IL  +PF SA+QRM+ + ++ G ++   + KGAPE +A  C+ ET+P +F S L +YT QG RVI  A+KSL +    K+  DL+R ++E    FLGL+IM+N +K ET   ++EL  A I +VMVTGDNI TA++V ++  ++ P   +I VEA  IP  +S  +    + E   + +   DS          + +   + FA  GK++ ++  +   LL +++    +FARM P QK  L+E  + L   V MCGDG NDCGALK AHAGISLS  EASVA+PFTSR   I CVP LIREGRA +V++F  FK+   +   Q + VL++++      + Q+L  D+ +  +  + +     +  LV  +P   L+S   L SVI  + F     +  +  VQ Q W+      +     N                              SYE T ++LLS    ++ A++FSKG+P+R+P++TN +  +  V  +    F+   + +D   +M+    P+  +R+ +V ++       +L E  +I+      +  W K   +      ++ L+  L   S WPP+ +
Sbjct:  267 EGFQELESHHLVPGDMVVL-KEGKALLPCDAILISGQCIVNESMLTGESIPVTKTQLPQADNLKPWKMHCAEDYKKHVLFCGTEVIQTKGDDRGVVKAVVLQTGFNTAKGDLVRSILYPKPMNFRLYRDALRFLMCLIAFAAIGMIYTVCVFALNGEEAGEVVKKALDVITIAVPPALPAALTTGIIYTQRRLKKKGIFCISPQRINMCGQLNLICFDKTGTLTEDGLDLWGLLPSEGNCFQDVRRFPADHSLPWGPAFRAMVVCHSLIVLEGKIQGDPLDVKMFEATNWVIDDSSGHQIEGQRSTHATVIRPGPKAN--------TASVD-GITILHQFPFSSALQRMSVIAQETGGEK-QAFTKGAPEMVATLCRAETVPSNFESKLLFYTAQGFRVIGLAYKSLQSG---KQSTDLTREEVESDLTFLGLLIMENRLKRETKPVLEELSAAHIRSVMVTGDNIQTAVTVAKNAGMISPTNRVILVEANKIPGSFSASVTWKPLKENKTEDDGNLDSGSQTGRRIRLAAEPGQFHFAMSGKSYQVVAQYFSHLLPKLLLNATVFARMSPSQKSSLVEEFQKLDYFVGMCGDGANDCGALKVAHAGISLSEQEASVASPFTSRTPSIACVPELIREGRAALVTSFCMFKYMALYSTIQYLGVLLLYWQLNSFGNYQFLFQDLAITTVIGMTMSFTEAYPKLVPYRPPSQLVSPPLLLSVILNILFSLGMQILGFLMVQKQPWYSKTDIHSACLSVNNHVENSSSASSLGLHGVGGGDPTEVDNGYKSYENTTVWLLSTINCLIIALVFSKGKPFRQPIYTNYVFIMVLVGQLGVCLFLVFADIDDLYSKMDLVCTPT-TWRISMVMMLAVTLAVSFLVEEAIIEN---RALWLWLKKTFQYHSKSHYKRLQRVLEQDSAWPPLNE 1174          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. SwissProt
Match: gi|189081268|sp|Q3TYU2.2|AT135_MOUSE (RecName: Full=Probable cation-transporting ATPase 13A5; AltName: Full=P5-ATPase isoform 5)

HSP 1 Score: 567.77 bits (1462), Expect = 1.355e-180
Identity = 347/934 (37.15%), Postives = 524/934 (56.10%), Query Frame = 0

Query:  185 ELSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNV----QFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALD-SRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMA-TPTLVAP-PKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEA----ELIPDGYSQGLNVSYMLEENEKSNIVHDSNFIKSVQE-KNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVP-YKFEAGL----------------WPPNP--------SYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRL-VIVGVMVFNCIFCYLWEMYLIDGVLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWPPIGDCKNNEIKIAVSMQSG 1080
            EL S  LVPGD+++LP  G + + CDAIL++G+CVVNE MLTGESIP+TK  +P   N       + +  R+HV+FCGTE++Q K      ++AVV++TG+ T KG+LVR+IL+P PL+F+ ++D  K +     +G++G  Y+L +++ +    +E    +L +L+  VPP+LPAALT  N +AQKRL+K  IFC+  + I +CG I++V FDKTGTLTE  LDL G        FQ   +  +  A+    L  AM +CHSLI L G + G+PLD+K+FE   W ++D         +GMA + T++ P PK S+        +P +  I IL+ +PF S +QRM+ + +  G     VY+KGAPE +A  C+ ET+P +F+  L+ YT QG RVIA AHK+L    R  ++D L+R  +E +  FLGL+IM+N +K ET   +KEL EA I TVMVTGDN+ TAI+V ++ E++     ++ VEA    +L+P   +  L  +      +K   +   N   SV   K Y FA  GK++ ++ +H  ++L +I+  G IFARM P QK  L+E  + L   V MCGDG NDCGALK AHAGISLS  EASVA+PFTS+  +I CVP+LIREGRA +VS+F  FK+   +   Q I   ++++      + QYL+ D+ +  +  + +     +  L   +P   LLS   L SV     F  +  V  +  V+ Q W+   YK+                    W  N         S+E T ++ +     + AA IFSKG+P+RKP++ N + ++   +A   T F+   + +D   +M F IP    +R+ +++   V  C+  ++ +  L +  L+  I   +    +     +  L+ +L   S WPP+     N    AV+ ++G
Sbjct:  275 ELESRLLVPGDILILP--GKISLPCDAILIDGSCVVNEGMLTGESIPVTKTPLPQTENTMPWKSHSLEDYRKHVLFCGTEVIQVKPSAQGLVRAVVLQTGYNTAKGDLVRSILYPRPLNFKLYNDAFKFMVFLACVGVVGFFYALGVYMYHEVPPRETATMALILLSATVPPVLPAALTIGNVYAQKRLKKEKIFCISPQRINMCGQINLVCFDKTGTLTEDGLDLWGTVPTAGNCFQAVHSFASGEAVPWGPLCAAMTSCHSLILLDGTIQGDPLDLKMFEGTGWNMEDSQVASCK--FGMADSSTVIKPGPKASQ--------SPVD-SITILRQFPFSSGLQRMSVIAQLAGDLHLHVYMKGAPEMVARFCRSETVPKNFSQELRNYTVQGFRVIALAHKTLKM-ERLSDMDHLAREKVESELAFLGLLIMENRLKKETRPVLKELSEARIRTVMVTGDNLQTAITVAKNSEMIPVGSQVVIVEANEPGDLVPASVTWQLVGTQEPGSGKKDTYIDIGN--SSVPAGKGYHFAMSGKSYQVLFHHFYSMLPQILVNGTIFARMSPGQKSSLVEEFQKLNYYVGMCGDGANDCGALKMAHAGISLSEQEASVASPFTSKTANIECVPHLIREGRAALVSSFGVFKYLTMYGIIQFIGTSLLYWQLQLFGNYQYLLQDVAITLMVSLTMSINHAYPKLAPYRPAGQLLSPQLLLSVFMNSCFTCIVQVCTFLTVKQQPWYCEVYKYSECFLVNQSNLSANVSLDRNWTGNATLVPASVLSFEGTTLWPIVTFNCISAAFIFSKGKPFRKPIYANYLFSLLLASAAGLTIFILFCDFQDLYRKMEF-IPTPTSWRVSILIAAFVQFCVAFFVEDAVLQNRELWLFIKKEFGFYSKS---QYRILQRKLAEDSTWPPV-----NRTDYAVNGKNG 1183          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. SwissProt
Match: gi|341940584|sp|Q9CTG6.3|AT132_MOUSE (RecName: Full=Cation-transporting ATPase 13A2)

HSP 1 Score: 559.296 bits (1440), Expect = 6.814e-178
Identity = 330/884 (37.33%), Postives = 490/884 (55.43%), Query Frame = 0

Query:  186 LSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSNIVHDSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWP--PIGDCK 1067
            + S  LVPGD ++LP  GGVM  CDA LV G CVVNES LTGES P+ K ++P E    +  +  R+H +FCGT ILQ +A +G  + AVV RTGF T KG LV +IL P P+ F+F+   +K +     L L+G  YS+ +   N   ++E ++ +LD++T VVPP LPAA+T    +AQ RL+  GIFC+H   I L G + +V FDKTGTLTE  LD+ GV  +K         +P  L L   L++A+ATCH+L +L     G+P+D+K+ E+  W L++    G  P  G     ++ PP G     G  +     + +++L  +PF SA+QRM  V    G+ Q + Y+KG+PE +A  C PET+P DF+  LQ YT  G RV+A A K L           L+R  +E++   LGL++M+NL+K +T   I+ L +  I TVMVTGDN+ TA++V R C +V   + +  + A     G    L      E +   N    + +    + ++   A  G TFA++R H   LL +++ +  +FARM PEQK  L+  ++ L   V MCGDG NDCGALK A  GISLS AEASV +PFTS    I CVP +IREGR ++ ++F+ FK+   +  TQ I+VL+++ I T   D Q+L +D+ +     +++   GP  +LV+ +P   LLS   L S++  +       +G +F V  Q WFVP           P+YE T +F LS    ++ A   SKG P+R+PL+TN    V  +A + +     +         +  +      F+L+++G++ FN +  ++ E  L D  L A  L W + + R     F+ L++EL ++  WP  P+G  +
Sbjct:  302 VDSSELVPGDCLVLPQEGGVM-PCDAALVAGECVVNESSLTGESTPVLKTALP-EGPKPYCPETHRRHTLFCGTLILQARAYVGPRVLAVVTRTGFCTAKGGLVSSILHPRPISFKFYKHSMKFVAALSVLALLGTVYSIIILYRNRVPVREIVIRALDLVTVVVPPALPAAMTVCTLYAQSRLRTQGIFCIHPLRINLGGKLRLVCFDKTGTLTEDGLDVMGVVPLKGQVLLPLVPEPCHLPLGP-LLRALATCHALSQLHDTPVGDPMDLKMVESTGWVLEEGPAAGSAP--GSQVLVVMRPPPG-----GPRQQEEPPVPVSVLCRFPFSSALQRMDVVVTWPGATQPEAYVKGSPELVASLCSPETVPSDFSQVLQSYTAAGYRVVALAGKPLPIAPSLAAAQQLTRDTVERELSLLGLLVMRNLLKPQTAPVIQTLRKTGIRTVMVTGDNLQTAVTVARACGMVGAQEHLAVIHATHPEQGQPAALEF-LPTESSAVMNGAKATGYPTVPEPQSCHLALSGSTFAVLRKHFPKLLPKVLVQATVFARMAPEQKTELVCELQRLQYCVGMCGDGANDCGALKAADVGISLSQAEASVVSPFTSSMASIECVPTVIREGRCSLDTSFSVFKYMALYSLTQFISVLILYTINTNLGDLQFLAIDLVITTTVAVLMSRTGPALTLVRARPPGALLSVPVLGSLLLQVALVAGIQLGGYFLVIAQPWFVPLNRTVPAPDNLPNYENTVVFSLSGFQYLILAAAVSKGAPFRQPLYTNVPFLV-ALALLGSVLVGLILVPGLLQGPLGLRNIVDSSFKLLLLGLVAFNFVGAFMLESVL-DQCLPA-CLRWLRPK-RASKKQFKRLQQEL-AEHPWPTLPVGSVR 1169          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. SwissProt
Match: gi|14285364|sp|Q9NQ11.2|AT132_HUMAN (RecName: Full=Cation-transporting ATPase 13A2)

HSP 1 Score: 558.14 bits (1437), Expect = 3.272e-177
Identity = 309/781 (39.56%), Postives = 450/781 (57.62%), Query Frame = 0

Query:  186 LSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSNIVHD----SNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTN 962
            + S  LVPGD ++LP  GG +M CDA LV G C+VNES LTGESIP+ K ++P E    +  +  R+H +FCGT ILQ +A +G  + AVV RTGF T KG LV +IL P P++F+F+   +K +     L L+G  YS+++   N   + E ++ +LD++T VVPP LPAA+T    +AQ RL++ GIFC+H   I L G + +V FDKTGTLTE  LD+ GV  +K   F     +P +L +   L++A+ATCH+L +L+    G+P+D+K+ E+  W L+++     +  +G     ++ PP         ++  P  + +++L  +PF SA+QRM+ V    G+ Q + Y+KG+PE +AG C PET+P DFA  LQ YT  G RV+A A K L      +    L+R  +E     LGL++M+NL+K +T   I+ L    I  VMVTGDN+ TA++V R C +V P + +I V A     G    L    M E     N V D    +++      ++   A  G TF +I  H   LL +++ +G +FARM PEQK  L+  ++ L   V MCGDG NDCGALK A  GISLS AEASV +PFTS    I CVP +IREGR ++ ++F+ FK+   +  TQ I+VL+++ I T   D Q+L +D+ +     +++   GP   L + +P   LLS   L S++  +   T   +G +F    Q WFVP           P+YE T +F LS    ++ A   SKG P+R+PL+TN
Sbjct:  307 VDSSELVPGDCLVLPQEGG-LMPCDAALVAGECMVNESSLTGESIPVLKTALP-EGLGPYCAETHRRHTLFCGTLILQARAYVGPHVLAVVTRTGFCTAKGGLVSSILHPRPINFKFYKHSMKFVAALSVLALLGTIYSIFILYRNRVPLNEIVIRALDLVTVVVPPALPAAMTVCTLYAQSRLRRQGIFCIHPLRINLGGKLQLVCFDKTGTLTEDGLDVMGVVPLKGQAFLPLVPEPRRLPVGP-LLRALATCHALSRLQDTPVGDPMDLKMVESTGWVLEEEP--AADSAFGTQVLAVMRPPLWEPQLQA-MEEPP--VPVSVLHRFPFSSALQRMSVVVAWPGATQPEAYVKGSPELVAGLCNPETVPTDFAQMLQSYTAAGYRVVALASKPLPTVPSLEAAQQLTRDTVEGDLSLLGLLVMRNLLKPQTTPVIQALRRTRIRAVMVTGDNLQTAVTVARGCGMVAPQEHLIIVHATHPERGQPASLEFLPM-ESPTAVNGVKDPDQAASYTVEPDPRSRHLALSGPTFGIIVKHFPKLLPKVLVQGTVFARMAPEQKTELVCELQKLQYCVGMCGDGANDCGALKAADVGISLSQAEASVVSPFTSSMASIECVPMVIREGRCSLDTSFSVFKYMALYSLTQFISVLILYTINTNLGDLQFLAIDLVITTTVAVLMSRTGPALVLGRVRPPGALLSVPVLSSLLLQMVLVTGVQLGGYFLTLAQPWFVPLNRTVAAPDNLPNYENTVVFSLSSFQYLILAAAVSKGAPFRRPLYTN 1078          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. SwissProt
Match: gi|30581066|sp|Q27533.2|YH2M_CAEEL (RecName: Full=Probable cation-transporting ATPase W08D2.5)

HSP 1 Score: 531.561 bits (1368), Expect = 2.160e-166
Identity = 320/915 (34.97%), Postives = 486/915 (53.11%), Query Frame = 0

Query:  186 LSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDE-NNVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQ------ADPTQ-------------LALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWEL---QDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFD---VYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEA-----------------------ELIPD--------------GYSQGLNVSYMLEENEKSNIVHDSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAGLWPP-------NPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVLFA 1030
            + S  LVPGD++L+P +G  +M+CD++L+ GT +VNES+LTGES+PITK+++ DE N+  F  +   ++V+FCGT++LQ +   G  +KA+V+RT + T KG+LVR+I++P P+DF+F  D  K I     +   G  Y++ + I  G T++  ++ SLDI+T  VPP LPAA++     AQ RL+K  IFC+    I  CG I+VV FDKTGTLTE  LD   V  V +A  QE Q      ++ T+             L  D  LV+A+ATCHSL ++ G L G+PLD+ LF+   W +    +         +    P+++ P            T   + E ++++ + F S++QRM+ +       + D   +Y KG+PE I   C P T+P D+   +  Y + G R+IA A + L+ N  + +   + R  +E   + LGLI+M+N VK  T G I +L+ A+I TVMVTGDN+LT +SV R+C +++P +    VE                        E+I D              G S+G   S    E E  N V  ++ +  +   +Y  A  G TFA+I +    L+D++     +FARM P+QK  L+E ++ +   V MCGDG NDC ALK AHAGISLS AEAS+AAPFTS+   I CVP +I EGRA +V++F  FK+   +  TQ + V+ +++I    +D Q++ +D+ L  +  ++ GN      L    P   LLS   + SV+  L    +    V+F    Q WF PY+      PP         S + T +F +S    ++ A+++SKG P+R  L++N  M   T+ A     F+ ++ +E     +     PSL FR+ IV V   N    Y +E   +D  L  
Sbjct:  257 IGSDQLVPGDILLIPPHG-CLMQCDSVLMNGTVIVNESVLTGESVPITKVALTDETNDSVFNIEKNSKNVLFCGTQVLQTRFYRGKKVKAIVLRTAYSTLKGQLVRSIMYPKPVDFRFTKDLFKFILFLACISGCGFIYTIIVMIMRGNTLRRIIVRSLDIITITVPPALPAAMSVGIINAQLRLKKKEIFCISPSTINTCGAINVVCFDKTGTLTEDGLDFHVVRPVMSAVNQEIQKVKLEKSNRTEFMGEMTELTSRNGLPFDGDLVKAIATCHSLTRINGVLHGDPLDLILFQKTGWTMEEGIEGDIEEETQRFDNVQPSIIKP------------TDDKSAEYSVIRQFTFSSSLQRMSVIVFDPREDRPDNMMLYSKGSPEMILSLCDPNTVPEDYLLQVNSYAQHGFRLIAVARRPLDLN--FNKASKVKRDAVECDLEMLGLIVMENRVKPVTLGVINQLNRANIRTVMVTGDNLLTGLSVARECGIIRPSKRAFLVEHVPGELDEYGRTKIFVKQSVSSSDEVIEDDASVSISMCSSTWKGSSEGDGFSPTNTEVETPNPV-TADSLGHLIASSYHLAISGPTFAVIVHEYPELVDQLCSVCDVFARMAPDQKQSLVEQLQQIDYTVAMCGDGANDCAALKAAHAGISLSDAEASIAAPFTSKVPDIRCVPTVISEGRAALVTSFGIFKYMAGYSLTQFVTVMHLYWISNILTDGQFMYIDMFLITMFALLFGNTPAFYRLAHTPPPTRLLSIASMTSVVGQLIIIGVVQFIVFFSTSQQPWFTPYQ------PPVDDEVEDKRSMQGTALFCVSMFQYIILALVYSKGPPFRGNLWSNKPMCALTIFATLLCLFIVIWPTELVLKTLGNVELPSLTFRIFIVIVGAVNAAVSYGFETLFVDFFLLG 1149          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. nr
Match: gi|1325271674|ref|XP_023320447.1| (probable cation-transporting ATPase 13A3 [Eurytemora affinis])

HSP 1 Score: 1332.01 bits (3446), Expect = 0.000e+0
Identity = 659/980 (67.24%), Postives = 775/980 (79.08%), Query Frame = 0

Query:  186 LSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKT---APSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSNIVHDSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLV-IVGVMVFNCIFCYLWEMYLIDGVLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWPPIGDCKNNEIKIAVSMQSGNSSFRVTTPTTTIATSTHNKNNPGALESQGRVATQIKHASRE------VTRNIKNRWSASSPQMEEEHLLASVDTPHATPL 1155
            LSS+ LVPGD ILLP  GG  +ECD++LVEG+ VVNESMLTGESIP+TK+ IPDE  V F YD  RQ+++FCGTE++QGKA+ GN+IKAVVIRTGFMTTKGELVRAILFPPPLDF+F SDFLKS+YVFLTLGLIGM YS+ MWI NGGTI E LLNSLDILTFVVPPILPAALTANNAFAQ+RLQK GIFCLH+KHI LCGGIDVVAFDKTGTLTEG+LDLAGVCE+K   FQE++ +P++L  DS L+Q MA+CHSLIK+ GELTG P+D+KLFEAI WEL+++ + G+NP+YG+ATPTLV+PPK  R+G G +K    APSNLEIA+LK YPFDSAVQRMT V KKKGS  + V+IKGAPEK+A  CKPETIP  F + LQ+YTKQG RVI AA   L    +  EV+ LSRS+LE  ADFLGLIIMQNLVK ETYGAIKELH+ADI+TVMVTGDNILTAISVGRDCELVKPDQTI+RV       G  Q   V Y   E E ++I+HD+  +KS+   +YVFACDG TF  I   D  L  +IV RGKIFARMLPEQKIHLIE +K++GRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNV+I CVPYLIREGRATMVSAFASFKFGVAFCFTQLI+VLMVFYIGTEPSDNQYLVVDIGLAA+PI+MIGN  PH+ LVK+KP RHLLSFLP+FSV+SFLFFQT+ Y+ +WFYVQ Q WFV Y+F + LWPPN SYEQTNIFL +C+AA +AAIIFSKG PYRKPL+TNGIMA WT+AAV+   FM+L+ +EDFA R+NFKI P  EF+++ ++G+ V  C FCYLWE+Y++DG LF K+LPWYK  +RGP+L FEHLE++L S   WPPIG+   N+ K +     G        P   +A S ++K       SQ  V     H SR+       T+N++ R S  S  + E     + + P +TP+
Sbjct:  228 LSSVDLVPGDTILLPATGGYTIECDSVLVEGSAVVNESMLTGESIPVTKVGIPDEREV-FNYDYNRQYIMFCGTEVMQGKAKQGNYIKAVVIRTGFMTTKGELVRAILFPPPLDFKFFSDFLKSVYVFLTLGLIGMGYSMAMWIRNGGTISEILLNSLDILTFVVPPILPAALTANNAFAQRRLQKRGIFCLHTKHISLCGGIDVVAFDKTGTLTEGDLDLAGVCEIKENQFQESEPNPSRLPTDSLLIQTMASCHSLIKMNGELTGYPMDIKLFEAIGWELKEEPHAGLNPEYGIATPTLVSPPKNIRSG-GQLKLGGFAPSNLEIALLKQYPFDSAVQRMTVVAKKKGSSNYSVFIKGAPEKVASLCKPETIPQGFTNLLQYYTKQGFRVIGAAVNGLKPGLKLSEVNGLSRSELEMGADFLGLIIMQNLVKKETYGAIKELHDADIHTVMVTGDNILTAISVGRDCELVKPDQTIVRVTLA----GTDQ-FQVQYTAPETETASIIHDTTQLKSLTGDSYVFACDGPTFNAIMTKDPELFKKIVHRGKIFARMLPEQKIHLIEALKNMGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVNISCVPYLIREGRATMVSAFASFKFGVAFCFTQLISVLMVFYIGTEPSDNQYLVVDIGLAAVPILMIGNTAPHDQLVKRKPTRHLLSFLPMFSVVSFLFFQTIAYMFIWFYVQSQEWFVKYQFVSDLWPPNSSYEQTNIFLYACAAATIAAIIFSKGSPYRKPLYTNGIMATWTLAAVSTVIFMSLYRTEDFAERLNFKIAPHFEFQVIAVIGIFVNFC-FCYLWEIYVLDGFLFQKVLPWYKDNVRGPNLEFEHLEKKLASNPSWPPIGEISTNKEKGSTEKYPG-------YPLPDVAASINSK-------SQFSVEPVSTHTSRKDKFLKNPTKNMRRRDSDGSKLLSE-----TCENPESTPV 1180          

HSP 2 Score: 121.324 bits (303), Expect = 6.563e-24
Identity = 72/170 (42.35%), Postives = 97/170 (57.06%), Query Frame = 0

Query:   13 SEEALVNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLKDKFEQIFEEDVVDHSSPEMTNNSSPICSFFINKKIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLGQLTVTDRHQEYGANLIKISVPPVLYLVFHEA 182
            +E + +N G+ DEM + GF     + + T+++++LTGGL L+ +TWR DIK+ C+Y+ V L  A+KILL D FEQ+FEEDV+            P  S+F+NKKIKYVW     T+ KLK I                         + YG NLIKISVPP+LYLV HE 
Sbjct:   20 NEVSKINIGEPDEMGIGGFCMCTPRLIATIILIILTGGLALVFITWRKDIKMFCLYTAVPLSRARKILLTDNFEQVFEEDVI-------WVGERP--SYFVNKKIKYVWSETGETFQKLKTIQII----------------------EMYGDNLIKISVPPILYLVIHEG 158          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. nr
Match: gi|321469594|gb|EFX80574.1| (hypothetical protein DAPPUDRAFT_318475 [Daphnia pulex])

HSP 1 Score: 816.609 bits (2108), Expect = 0.000e+0
Identity = 460/1120 (41.07%), Postives = 656/1120 (58.57%), Query Frame = 0

Query:   18 VNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLKDKFEQIFEEDVVDHSSPEMTNNSSPICSFFINKKIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLGQLTVTDRHQEYGANLIKISVPPVLYLVFHE-----------------AKTELSSMALVPGDVI-------------LLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISI-PDENNVQFAYD-------------------------------------LQRQHVVFCGTEILQGKAQLGN-FIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGY--SQGLNVSYMLEENEKSNIVHDSNFIK--SVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWPPIG 1064
            +N    +E+ V G+  +  +  +    ++LT GL L+LL WR DIK+   Y +  L+ A KIL+KD F   +EED  +     +     P   FF+NKK+KY+WD     + +LK  DE  +   FH+   GLG   V +RHQ+YGAN I+I+V PV  L+  E                 A++     ALV    +             ++     V +   A +V G   V + +LT + +P   + I P E+ V F  D                                     + ++H++F GT++LQG+ Q  N F+KAVVIRTGFMT+KGEL+RAILFP P+ F+F++D ++   +FL                  G  +E +L+SLDI+TFVVPP+LPAALTA  AFAQ+RL++  IFCL +KHI L GG+DV  FDKTGTLTE ++DLAG   ++   F++     + L+    L+QA ATCH+LIK+  +L G  +D K+F+A +W   D    GVN DYG+ TP LV+ P  + N      +    +E  +LK +PF+S ++RMT + ++KG+++++V+IKGAPE IA  C P T+P ++ + L+ YT QG RVIA A K+L+ +  W  +  ++R ++E   + +GL+IM+N +K ET  AI+ LHEA I TVMVTGDN+ TA++V +DC+++   Q II+VEA +IP     +Q L V Y       ++ +    FI    V   NY FA DG +F L+R HD ALLD+ + RGK+FARM PE K HLIE ++ +GRQV M GDGCNDCGAL+TA AGISLSMA+ASVAAPFTS+  +I CV  LI EGR T+ +AF +F+F +  CF   + VLM++ I T PSD QY++ D G+A +P   IGN  P   L  ++P+RHL +FLPLFS  +FL +QT+  +  WFY   Q WF P+ FE G  PPNP YE+T IF L C  ++VAA++FS  EPY +  FTN I   W + A  A   +    +E F +  NFK PP  E+ +VI+ + +   +FC+ WE YLI G ++  + P    RIR P   ++ +E+EL+SK  WP +G
Sbjct:   11 INADGTNELEVRGYRVHRGRCALACAALILTLGLLLVLLVWRKDIKMWMFYKECSLEHASKILVKDTFGVFYEEDAAEERCGPILE---PKTRFFVNKKVKYIWDKKTLQFNRLKCYDENAEFQSFHENPGGLGLQEVNERHQKYGANFIRITVLPVYRLILKEISNPFYLFQFYTIVVWMAQSYYDYSALVLATTMIAVGSSVYETRKHMVSLKKKVQVAGSATVVRGG--VEKRILTQQLVPGDILCINPLEDKVPFHCDAVLVEGTCSVDESMLTGESYPITKMPVPEDRETFEYEVHKRHILFNGTQLLQGRPQGNNVFLKAVVIRTGFMTSKGELIRAILFPKPIHFRFYADLIQVAILFL------------------GNTKEIILHSLDIVTFVVPPMLPAALTATTAFAQRRLREKKIFCLSAKHISLSGGVDVACFDKTGTLTETDIDLAGAIPIQEGEFRKPVLQLSSLSEAHPLLQAAATCHTLIKVNDQLNGYSIDRKMFDATKWNFADGPT-GVNADYGVETPYLVSSPSWNENQES---SPHPTVEYGLLKRFPFESTIKRMTVIAQRKGNERYNVFIKGAPEIIAELCDPVTVPSNYYAVLKHYTIQGYRVIALAVKTLSPHFTWTHIQQMTRDEVEANPELIGLLIMRNQLKKETIPAIRILHEAHIRTVMVTGDNLQTAVTVAKDCDMIDRVQRIIQVEAAIIPASIHGAQHLQVLY-------NDPLAMPEFIAGTDVHNGNYCFAMDGPSFELLRIHDPALLDKCIHRGKVFARMAPEHKQHLIEALQKIGRQVAMVGDGCNDCGALRTADAGISLSMADASVAAPFTSQETNISCVAPLICEGRTTLDAAFGTFQFAIGICFIFFVGVLMLYSISTTPSDFQYVIWDFGIAIVPFFTIGNTTPPKYLHPRRPLRHLWAFLPLFSFFTFLGWQTVIMLIGWFYCHAQPWFEPFVFEPGKHPPNPCYEETTIFYLMCIGSIVAALVFSPSEPYSRSSFTNKIFIAWFLEATVAVLCVMFITNEGFVYWCNFKTPPHTEYTVVILVLGLAGGLFCFCWEKYLIRGWMYHWVWPRIN-RIRPPRHTYQKIEKELKSKPDWPKLG 1095          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. nr
Match: gi|1009554765|ref|XP_015912737.1| (probable cation-transporting ATPase 13A3 isoform X2 [Parasteatoda tepidariorum])

HSP 1 Score: 751.51 bits (1939), Expect = 0.000e+0
Identity = 386/894 (43.18%), Postives = 568/894 (63.53%), Query Frame = 0

Query:  187 SSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQFAYDL--QRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQ-----GLNVSYMLEE----NEKSNIVH----DSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAGL-WPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWPPIG 1064
            SS  LVPGDVI+LP    +M+ CDA+L+ G CVVNESMLTGES PITK+  P  N++   YD    +++ +FCGTE+L  +   G+ IKA+V RTGF T KGELVRAILFP P++F+ +++  K + +F  LG+  + Y+  +W+  G   ++ ++  +D+LTF+VPP+LPA LT+ NA AQ+RL+K GI+CL+S++I  CGG+DVV FDKTGTLTE +LD++GV  + +  F+        L  +S L++AMATCHSL K+ G+L G+ +DVK+FEAI W+L++       PD+G   P    PP+                 IA+++ +PF+S ++RM+ V K +GS+ F+VY+KGAPE ++   KPET+P +F   L++YT+QG RV+   +K L  +  W +V  L R +LE    F GLI++QN +K ET   +  L  ADI T+MVTGDN+LTAI+V RDC +++   ++I V+A ++    S+      L + Y   +    +EK N+V     D     +++  NY  A +G TF LIR H+  LLD++V +G IFARMLPE K++LIE +++LG QV MCGDG NDCGALKTAHAG+SLS+AEASVA+PFTS   +I C+P +IREGRAT+ + F +F++   +CF  L AVL++F+ G +PSD  Y+V+DI L  LP I+ G+      LVK+ P R ++SFLP FS+ SF+  Q   YV  + Y  +Q W+ P+ +   +   P  S+  T I  ++  + V+AA+IF+ G PYRK   +N    V   A     ++ T++ ++  A  +N K  P +EF L++  + + N I  Y+WE++ I G LF  ++P  + R RGP   FE L+ ++ S   WPP+ 
Sbjct:  186 SSDELVPGDVIVLPETQFIMV-CDAVLLSGNCVVNESMLTGESTPITKV--PIANDISSHYDTANNKRNTLFCGTEVLHSRNVDGSGIKAIVYRTGFSTAKGELVRAILFPKPVNFKLYTELFKCMIIFFVLGIPPIIYTSIIWVHLGAYTRDTIIIVVDVLTFLVPPVLPAVLTSINAHAQRRLRKHGIYCLNSRYINFCGGLDVVCFDKTGTLTEDSLDISGVVPIDDGRFERAVKHLNHLP-NSPLMKAMATCHSLSKVNGKLLGHTVDVKIFEAIGWDLEE-------PDFGDQVPFERVPPRIVYPQPTETSPYQPASAIAVVRQFPFESLLRRMSVVAKSQGSRHFEVYLKGAPELVSSLSKPETVPDNFKEVLEFYTRQGFRVLGIGNKVLPDDFVWDDVLRLPREELECDMQFQGLIVLQNRLKPETVPTLNVLKAADIRTIMVTGDNLLTAITVARDCGMIEEYDSVISVQASIVGTESSKMDDKPRLQIHYNYAKLPGFSEKLNMVQNGMIDDICAPTMENSNYHLAMEGTTFNLIRLHNTQLLDKMVLKGTIFARMLPEHKLYLIETLQNLGHQVGMCGDGANDCGALKTAHAGVSLSVAEASVASPFTSTQQNIQCIPMVIREGRATLSATFGAFRYMTCYCFVLLAAVLIMFWDGQKPSDGGYVVIDIVLNLLPPILFGSTEAFPGLVKRAPTRSIISFLPQFSMFSFMIIQIGVYVLAYEYCLMQPWYEPFVYNKTMTHTPAASHSGTAILSVNMMSYVIAAVIFAPGPPYRKNFTSNKTYLVVVFAEFLLVTYFTIYPADSVARFINLKHAPFIEFHLLLYVLSLANFIISYIWEIFFIQGFLFDYVVPAIR-RFRGPIHKFEKLQLQMASNKKWPPVS 1067          

HSP 2 Score: 69.707 bits (169), Expect = 4.447e-8
Identity = 42/117 (35.90%), Postives = 59/117 (50.43%), Query Frame = 0

Query:   68 YSKVELKEAQKILLKDKFEQIFEEDVVDHSSPEMTNNSSPICSFFINKKIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLGQLTVTDRHQEYGANLIKISVPPVLYLVFHEAKT 184
            + +  L  A+K+LL D + Q F E V     P  +  SS   ++F NKKIKY+WD     + K+  + +    A FHK  +GL       R Q YG N I + V PVL LV  E ++
Sbjct:    5 HKRCSLATAEKVLLIDCYNQQFVEKV---ERPTDSTLSSKQYTYFFNKKIKYIWDQQQVKFYKVSGL-QIKNCASFHKLADGLHTTEAVFRLQHYGRNSIFVEVQPVLKLVLREIRS 117          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. nr
Match: gi|1009554762|ref|XP_015912736.1| (probable cation-transporting ATPase 13A3 isoform X1 [Parasteatoda tepidariorum])

HSP 1 Score: 751.125 bits (1938), Expect = 0.000e+0
Identity = 386/893 (43.23%), Postives = 568/893 (63.61%), Query Frame = 0

Query:  187 SSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQFAYDL--QRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQ-----GLNVSYMLEE----NEKSNIVH----DSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAGL-WPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWPPI 1063
            SS  LVPGDVI+LP    +M+ CDA+L+ G CVVNESMLTGES PITK+  P  N++   YD    +++ +FCGTE+L  +   G+ IKA+V RTGF T KGELVRAILFP P++F+ +++  K + +F  LG+  + Y+  +W+  G   ++ ++  +D+LTF+VPP+LPA LT+ NA AQ+RL+K GI+CL+S++I  CGG+DVV FDKTGTLTE +LD++GV  + +  F+        L  +S L++AMATCHSL K+ G+L G+ +DVK+FEAI W+L++       PD+G   P    PP+                 IA+++ +PF+S ++RM+ V K +GS+ F+VY+KGAPE ++   KPET+P +F   L++YT+QG RV+   +K L  +  W +V  L R +LE    F GLI++QN +K ET   +  L  ADI T+MVTGDN+LTAI+V RDC +++   ++I V+A ++    S+      L + Y   +    +EK N+V     D     +++  NY  A +G TF LIR H+  LLD++V +G IFARMLPE K++LIE +++LG QV MCGDG NDCGALKTAHAG+SLS+AEASVA+PFTS   +I C+P +IREGRAT+ + F +F++   +CF  L AVL++F+ G +PSD  Y+V+DI L  LP I+ G+      LVK+ P R ++SFLP FS+ SF+  Q   YV  + Y  +Q W+ P+ +   +   P  S+  T I  ++  + V+AA+IF+ G PYRK   +N    V   A     ++ T++ ++  A  +N K  P +EF L++  + + N I  Y+WE++ I G LF  ++P  + R RGP   FE L+ ++ S   WPP+
Sbjct:  241 SSDELVPGDVIVLPETQFIMV-CDAVLLSGNCVVNESMLTGESTPITKV--PIANDISSHYDTANNKRNTLFCGTEVLHSRNVDGSGIKAIVYRTGFSTAKGELVRAILFPKPVNFKLYTELFKCMIIFFVLGIPPIIYTSIIWVHLGAYTRDTIIIVVDVLTFLVPPVLPAVLTSINAHAQRRLRKHGIYCLNSRYINFCGGLDVVCFDKTGTLTEDSLDISGVVPIDDGRFERAVKHLNHLP-NSPLMKAMATCHSLSKVNGKLLGHTVDVKIFEAIGWDLEE-------PDFGDQVPFERVPPRIVYPQPTETSPYQPASAIAVVRQFPFESLLRRMSVVAKSQGSRHFEVYLKGAPELVSSLSKPETVPDNFKEVLEFYTRQGFRVLGIGNKVLPDDFVWDDVLRLPREELECDMQFQGLIVLQNRLKPETVPTLNVLKAADIRTIMVTGDNLLTAITVARDCGMIEEYDSVISVQASIVGTESSKMDDKPRLQIHYNYAKLPGFSEKLNMVQNGMIDDICAPTMENSNYHLAMEGTTFNLIRLHNTQLLDKMVLKGTIFARMLPEHKLYLIETLQNLGHQVGMCGDGANDCGALKTAHAGVSLSVAEASVASPFTSTQQNIQCIPMVIREGRATLSATFGAFRYMTCYCFVLLAAVLIMFWDGQKPSDGGYVVIDIVLNLLPPILFGSTEAFPGLVKRAPTRSIISFLPQFSMFSFMIIQIGVYVLAYEYCLMQPWYEPFVYNKTMTHTPAASHSGTAILSVNMMSYVIAAVIFAPGPPYRKNFTSNKTYLVVVFAEFLLVTYFTIYPADSVARFINLKHAPFIEFHLLLYVLSLANFIISYIWEIFFIQGFLFDYVVPAIR-RFRGPIHKFEKLQLQMASNKKWPPV 1121          

HSP 2 Score: 102.834 bits (255), Expect = 2.623e-18
Identity = 62/171 (36.26%), Postives = 90/171 (52.63%), Query Frame = 0

Query:   14 EEALVNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLKDKFEQIFEEDVVDHSSPEMTNNSSPICSFFINKKIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLGQLTVTDRHQEYGANLIKISVPPVLYLVFHEAKT 184
            E+  VN G  DEM + GF  +  K ++T + V+L+ GL L +L+WR  I +   + +  L  A+K+LL D + Q F E V     P  +  SS   ++F NKKIKY+WD     + K+  + +    A FHK  +GL       R Q YG N I + V PVL LV  E ++
Sbjct:    6 EDDRVNIGTDDEMIIRGFRRSYPKIILTWIGVILSCGLLLAVLSWRRRIFMKFTHKRCSLATAEKVLLIDCYNQQFVEKV---ERPTDSTLSSKQYTYFFNKKIKYIWDQQQVKFYKVSGL-QIKNCASFHKLADGLHTTEAVFRLQHYGRNSIFVEVQPVLKLVLREIRS 172          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. nr
Match: gi|1067065221|ref|XP_018016584.1| (PREDICTED: probable cation-transporting ATPase 13A3 isoform X1 [Hyalella azteca])

HSP 1 Score: 706.057 bits (1821), Expect = 0.000e+0
Identity = 373/894 (41.72%), Postives = 549/894 (61.41%), Query Frame = 0

Query:  184 TELSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQFAYD--LQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNG--GTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQA-DPTQLALDSRLVQ-AMATCHSLIKLKG-----ELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEENEKSNIVHDSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTY-VGVWFYVQIQSWFVPYKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWPPIGD 1065
            T +SS  LVPGD ++L    G+ +E D  L++G  VVNE++LTGES+PI K+ +   +N Q   +  L + H+++ G+EILQ +A        VV+ TGF T +GELVR+ILFP P+ F  +SDF+K +  F+ LG   MA S+YMW+      T+QE + NSLD+LTFV+PPILPA +TA N +AQ+RL+K  I+C+ S +I L G +DVV FDKTGTLTE +L+LA V       F E +  +   ++ +  L++  + +CH+L           L G+P+++++FE+++WEL+++ +  +NPDYG+ TP L+ P +  R          S+LE+A +KT+PFD+  QRMT V + KGS++F V+IKGA E +   C P  IP +F   L WY+ QGLRV+A A +S +A   W EV+   R  LE +A  LGL++  N  K ET   +  LH A I+TVM+TGD++ TAISV R C LVK  + ++ V          + L VSY +     +    D + I+  ++   VF  DG T+  IR HD+ LL ++  RG++FARM P+QK+ +IE ++DLGR V MCGDGCNDCGALK AH G+SL+  EASVAAPFTS++  I C+  LIREGRAT+VS +ASFK+ +++ F  LI V++++ I TEPSD QYL++D+ L  +P +++GN      L  ++P R +L  L + S++SFL  Q   + +  WF +Q Q WF  + F  G  P +PS+E T + + S    +  A+ FS   PYRK  +TN I+  + + A +   F T F  E+F   MNF   P   FR V+    + N + C +WE   + G +   ++PW   R+  P LP++ LE++++S + WP I D
Sbjct:  262 TVMSSRELVPGDTVILL--DGMALEVDVALIDGAAVVNEAILTGESVPIIKVGLSQADNPQQIINDSLHKMHILYSGSEILQLRA--ATCAIGVVLNTGFSTCRGELVRSILFPKPIAFHLYSDFMKMLGCFVVLGFGAMAGSIYMWLHTEPPATLQEVIFNSLDLLTFVIPPILPATMTAINVWAQRRLKKNNIYCVSSNYISLAGPVDVVCFDKTGTLTEDDLELACVVPCSEDGFFEKECRNLRDISAEQDLLRIGLGSCHTLTHYTSGAAPHNLIGHPMELRIFESLDWELEERKDGCINPDYGILTPVLLRPSEEQR----------SSLEVAQIKTFPFDANDQRMTVVAQIKGSREFHVFIKGAGETLKQICHPSKIPGNFDEVLTWYSLQGLRVVALAVRSWSAT--WHEVESKQRVQLESEARLLGLVLFVNPTKRETPLCLSILHHAHIDTVMITGDSLATAISVARSCGLVKTHEQMVIVRTNSTGSSVRR-LEVSYNVAFTASAK---DDDKIEIAEKDGLVFVMDGHTWDDIRRHDRKLLAKLALRGRVFARMKPDQKVQIIEELRDLGRHVAMCGDGCNDCGALKAAHVGVSLAATEASVAAPFTSKDPSIKCMDLLIREGRATLVSTYASFKYNISYGFASLIVVMILYSIRTEPSDAQYLIMDLVLITVPSLVLGNTAAPQLLGVERPTRRILHPLHISSILSFLLIQAGAFWLCFWFTMQ-QPWFEEFVFVPGQHPHSPSFEGTALVIYSFYTFINGAVCFSHSSPYRKSFYTNHILTAYLLLATSTVIFFTFFSGENFLKFMNFIDIPDAGFRWVLFLGALINLLVCLVWERLFLYGAIAEGLMPWLD-RVLPPRLPYQKLEKKIQSDNTWPVITD 1133          

HSP 2 Score: 106.686 bits (265), Expect = 2.245e-19
Identity = 64/180 (35.56%), Postives = 98/180 (54.44%), Query Frame = 0

Query:   14 EEALVNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLKDKFEQIFEEDVVDH--SSPEMTNNS--------SPICSFFINKKIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLGQLTVTDRHQEYGANLIKISVPPVLYLVFHEAK 183
            E   +N+G  DE+ ++GFV +  +  +T L  + TGGL L++LTWRA  +L C +    L  A KIL  D + Q +EEDV     SS E+T +          P   FF+NKKIKYVWD+ A+ +++L++ +        +  + GL    V  R + +G NLIKI V  +  L++ + K
Sbjct:   14 EPEKLNRGTNDEIAIQGFVKDWGRVAVTTLACVATGGLLLLVLTWRASFRLWCTHRPSTLDVATKILCTDIYGQEWEEDVASDVASSSEITKDGKVFRAPPEQPAVRFFVNKKIKYVWDNSAQMFIRLRSYESTQSLQGIYNNRHGLISEEVDRRKKLFGENLIKIEVLSIPQLLWQQIK 193          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. nr
Match: gi|1005956676|ref|XP_015786747.1| (PREDICTED: probable cation-transporting ATPase 13A3 isoform X1 [Tetranychus urticae])

HSP 1 Score: 705.286 bits (1819), Expect = 0.000e+0
Identity = 398/1127 (35.31%), Postives = 614/1127 (54.48%), Query Frame = 0

Query:   18 VNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLKDKFEQIFEEDVVDHSSPEMTNNSSPICSFFINKKIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLGQLTVTDRHQEYGANLI-------------KISVPPVLYLVF-----------------------------HEAK------------------------TELSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANH-RWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGLNVSYMLEE-NEKSNIVHDSNFIKS-------VQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAG-LWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDF-AHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDG----VLFAKILPWYKLRIRGPHLPFEHLEEELRSKSGWPPI 1063
            +N    DEM +EG++++  ++ +     LL+ G   +L TW+    +   + +  L +A +++L+D + + + E V+    P+ T       S F +KK+KY+W      + KL  +++  K    +   +GL       R   YG N I             +IS P  +Y +F                             HE +                        T  SS  LVPGD+I+L  N   ++ECDA+L+ G+C V+ESMLTGES PI+K+++ D+ NV ++ +  +++ +FCGTEI+  ++     +KA+V+RTG+ TTKGELVR IL+P P++FQ   D +K + +F  LG+  MAY+ Y+++S G ++ + ++  +DI TF+VPP+LPA +T+ N +AQKRL+K  IFCL +  I   G ID+V FDKTGTLTE ++D+AGV    N  F   +   T L   S LV  +A CHSLI+    L G+  ++++FE++E E +D   +  N  +      L+   +G           P +L   +++ +PFDS +QRM  V K+  +  + V +KGAPE I   C   TIP DF S L  YT+ G+RV+AAA +S++ +      +  + R DLE    F GLI+ QN +K ET   +  L E  I ++M TGDNILTA+SV RDC ++    ++++VEA L  +G  + L     L   +EK NI  DS    S       ++++ +  + +GK+F LIRN+D++LL +IV RG IFARMLP+ K+HLI+ ++  G QV MCGDG NDCGAL+ A+ GISLSMAE+SVA+PFT    +I CVP LIREGRAT+ +   +FK+  ++CF  L +VL++F+ G + SD  ++ +DI L   P ++ G    +  L + +P+  L  F PLFS+ SF+F Q + Y+    Y+ IQ WF P+ F A  +  P PS+    I  ++  + V+AAIIF+ G P+R    +N I        +     +T++ +  F    +NFK  P  EF+L +  + + N    YLWE+  +          K   W     R     +E +E EL+ +  WPP+
Sbjct:   28 INIDDDDEMIIEGYLWSLTRSFVFWFSGLLSAGFVFMLTTWKPSYLIKFTHKRCTLDQADRVILRDSYGEEYVEKVI---KPDYTRKERAQFSHFFHKKLKYLWRPELSQFSKLTGLEK-EKCGSIYSMSDGLTNSDAKIRQSLYGINSIIVQVDSITKLILSQISNPFYIYQIFIILVWMVQLYYQFAGCVLLFSIISITLHVHETRKQSIALRQKVHSESEVVVLREGIMTSKSSTELVPGDIIILAPNCSFVIECDAVLISGSCTVDESMLTGESEPISKVALTDDPNVLYSPNTHKKNTLFCGTEIIHVQSSDKIHVKALVVRTGYSTTKGELVRNILYPKPVNFQLKQDLIKCMILFFILGIPSMAYTAYIFVSYGASLYDTIIIVVDIATFLVPPLLPAVMTSINGYAQKRLRKADIFCLDTNFINAGGSIDMVCFDKTGTLTEDSIDIAGVVPSSNGLFGRPERKMTTLDRYSTLVAILANCHSLIERSRRLDGDRQEIRMFESVEAEFRDDTFVNENGAFERLPDRLIGFKRG-----------PDDLIYGLIRLFPFDSTLQRMAVVVKRAHTNSYSVLMKGAPEVIRTFCIDATIPNDFDSMLAMYTRDGMRVMAAASRSIDGDSIDLAGLMKIPREDLEHSMIFEGLIVFQNQLKPETESTLYALKEVGIRSIMATGDNILTALSVARDCGMIDEADSVVQVEANLTDNGSLETLYRYVKLPGISEKLNI-DDSTMKYSEEIITPILKKRCWHISIEGKSFELIRNYDRSLLSKIVHRGTIFARMLPDHKLHLIDEIQRQGHQVAMCGDGANDCGALRLANCGISLSMAESSVASPFTYTKKNIECVPILIREGRATLAATMGAFKYQASYCFVLLSSVLILFWEGAKLSDGGFVFIDIILNMFPPLVFGTTKAYPRLTRHRPLLSLFDFNPLFSIFSFVFIQVIIYLIAREYLLIQEWFEPFVFNATQIHRPEPSHMTLTILSVNTMSYVIAAIIFASGPPFRMSFLSNKIYTFVVFLNLLLVIIVTVYPTPGFLLDYVNFKDVPDFEFKLTLFSLGLANLGISYLWEVVFLQSGQSQQFCTKFTSW-----RNKKPLYEQIETELQLQDDWPPV 1133          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. nr
Match: gi|1242799627|ref|XP_022340300.1| (probable cation-transporting ATPase 13A3 isoform X3 [Crassostrea virginica])

HSP 1 Score: 701.819 bits (1810), Expect = 0.000e+0
Identity = 415/1164 (35.65%), Postives = 630/1164 (54.12%), Query Frame = 0

Query:   18 VNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLKDKFEQIFEEDV-VDHSS------------PEMTNNSSPICS-----------------FFINKKIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLG----------------QLTVT---------------------------DRHQEYGANLIKISVPPVLYLVFHEAKTE-----------------------LSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPD------ENNVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQL-ALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSG--NIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGL-NVSYMLEENEKSNIVHDSNFIK------SVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVLFAKILP-WYKLRIRGPHLPFEHLEEELRSKSGWPPIGDCKN 1068
            VN  Q D+M + G+  + ++ ++    +++T GL  +   W   + + C +SK  L EA+ ++L+D++ Q F   V ++H +              +T + +P  S                 +F+ KK+KY+W S ++T+ KL+ +D+  K + FH  K GL                 Q+ VT                           D +  Y + ++ ISV  +   ++   K +                       + S  LVPGD+I +P  G   M+CDA+L+ G C+VNESMLTGES+P+TK  +P+      + ++ F      +HV+FCGT+ILQ +      +KAVV+RTGF T KG+LVR+I++P P+DF+F+ D    + +   +  +G  Y++ + +      +E  L S+D++T  VPP LPAALT    FAQ RL+K  I+C+  + I + G I++V FDKTGTLTE  L+L  +  + +  F E   D  QL A  S L+ AMATCHSL  + G L+G+PLD+ +FEA  WEL++      +  Y M  PT+V P KGSRNG G  + +  P + EIAI++ + F S++QRM+ +T+K G+  F+++ KGAPE IA   KPET+P +F   L  YTK G RV+A A++SL A  ++ ++  + R  +E+   FLG ++M+N +K ET   I +L +A+I TVMVTGDN+LTA+SV R+C +V   + II V+A   P G  +    + YM  E+ +  +       K       V +  Y FA DG+++A+IR H   LL +   RG +FARM P+QK  LIE +++LG  V MCGDG NDCGALK AHAGISLS AEASVA+PFTS+  +I CVP LIR+GR  +V++F  FK+   +  TQ ++VL++++I    +D ++L +D+ L     +  G    ++ L K  P+  L S  P+ S+I  +  QT   V  +F VQ Q WFVPY  E        SYE   IFL+S    +  A++FSKG PYRK +F+N          V  + ++ ++  ED A     K+ PS+ +R V +G+ V N     L E Y+I+     + L  W +         +E  EEE+     WPP+   + 
Sbjct:   26 VNPAQEDQMEICGYRPSLIRKVMVWFFIIITAGLLRLFFYWLPHLFIKCTHSKCSLAEAEIVILRDQYLQWFVSRVKINHKNGMLGESAMKDGVAGITFSKTPFDSNTPLKMGDAQLNRKLVRYFVTKKVKYIWSSESQTFKKLEGLDKNTKCSYFHATK-GLNYSQQQERMSMFGSNSIQIHVTPIVKLLFKEVLSPFYIFQLFSCGIWFADEYYYYASCIVVISVVSITATIYQTRKMQRTLRNTIESSTIVSRLCGEEYHDICSDDLVPGDIISIPRQG-CTMQCDAVLLTGNCIVNESMLTGESVPVTKTPLPNPRVSKSQEDILFNMKEHSKHVLFCGTQILQTRFYGSQQVKAVVLRTGFSTAKGDLVRSIMYPKPVDFKFNRDTYIFVGILAAIAGVGFIYTVVLMVQEHEAFEEIFLRSMDLVTIAVPPALPAALTIGIVFAQSRLKKSQIYCISPRSINVSGTINIVCFDKTGTLTEDGLNLQSIVPLHDGRFAEEVYDVKQLPAEKSLLLNAMATCHSLTIINGVLSGDPLDLIMFEATGWELEEPGEEEGS-RYDMIVPTVVRP-KGSRNGKGAFDQQFVPVSDEIAIMRQFTFASSLQRMSVITRKLGAPNFELFSKGAPEMIASLSKPETVPSNFHEVLIEYTKHGYRVLALAYRSLPAKLKYAKIQRIQREQVERSLTFLGFLVMENRLKPETTPIIHQLLDANIRTVMVTGDNMLTALSVARECGMVSEHKKIILVQA--FPGGSGKSEPTLEYMYTEDPQDRLQKSEKETKIPMPDGHVTDSPYHFAMDGRSWAIIRKHFPLLLQKFCVRGTVFARMSPDQKSQLIETLQELGYFVGMCGDGANDCGALKVAHAGISLSEAEASVASPFTSKQPNIECVPTLIRQGRGALVTSFGIFKYMACYSLTQFVSVLILYWIFANLTDFEFLYIDLFLLTSLSVTFGYTNAYDKLSKDPPLVSLFSISPVLSIILHMAIQTAGQVFCYFNVQEQPWFVPY--EVNEDDDYTSYENMAIFLISMYQYITLAVVFSKGRPYRKTIFSNYFFMGNLAVCVGMSLWLNIYPIEDVATFFELKVAPSIPYRCVYLGIAVINFFLDLLVETYVIESYFVQERLQCWIEDIFSTSKYQYEIQEEEMVRDQPWPPVSSAET 1181          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. nr
Match: gi|1238885479|ref|XP_022255272.1| (probable cation-transporting ATPase 13A3 isoform X1 [Limulus polyphemus])

HSP 1 Score: 701.819 bits (1810), Expect = 0.000e+0
Identity = 362/792 (45.71%), Postives = 515/792 (65.03%), Query Frame = 0

Query:  187 SSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMA---TPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQG----LNVSYM----LEEN---EKSNIVHDSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVP--YKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTN 962
            SS  LVPGDVI LP +  VM ECDAIL+ GTCVV+ESMLTGES PITK+ I  ++N  ++    ++  +FCGTEIL   +   + +KA+V RTGF T KGELVR+ILFP P+ ++ +S+  KS+ +FL LG+  M Y+  +W  +G + +  ++  +D+ TFVVPP+LPA +T+ NA  Q+RL+   IFCL+S HI   GG+DVV FDKTGTLTE NLDLAG+  +++  FQ      +  +L S L+ A+ATCHSL  + G L G P+D+K+F  + WEL++        DY  +    P  +  PK        +   P   +IAI++ +PF+S +QRM+ +T K+GS  F++YIKGAPE ++  C   TIP +F   L+ YT+QG RV+A  ++SL  N  W E+    R DLE     +GL+++QN +K E+  +++ L +A+I TVMVTGDN+LTAISV RDC +V  + ++I VEAEL+  G S+     +N S++      E     K+ I+ D+  I  VQ   Y  A DGKTF LI+ +D+ LL ++V +G IFARMLPEQK+HLIE ++  G QV MCGDG NDCGALKTAHAG+SLS+AEASVA+PFT++  +I C+P LIREGRAT+ + F +F++ V +CF  L AVL++F+ G +PSD  Y+++DI L  LP I+ G    +  LVK+ P R +LSF+  FS+IS++  Q    +   +++ +Q WF P  Y  E  L PP PS+    I  ++  + ++AA+IF+ G PYRK  F+N
Sbjct:  242 SSHELVPGDVIFLPQHQCVM-ECDAILLSGTCVVSESMLTGESTPITKVPIAQDSNHPYSPLSNKRSTIFCGTEILHSCSSESSAVKALVYRTGFSTAKGELVRSILFPKPVQYKLYSELFKSMIIFLVLGIPAMVYTAIIWSHHGASAKNVVILVVDVATFVVPPLLPAVMTSINAHGQRRLRTHDIFCLNSGHINFAGGLDVVCFDKTGTLTEDNLDLAGIIPIQDVRFQHPFTPGSSHSLPSTLIHALATCHSLTVVDGRLMGYPVDIKMFNVVAWELEEP-----KADYHCSFERLPARIVRPKKKEAAFLRMSGMPVT-DIAIVRQFPFESLLQRMSVITCKEGSDNFELYIKGAPEMVSSFCDSNTIPENFLDVLELYTRQGFRVLALGYRSLEPNITWDEIFRKPRDDLECNLKLVGLLVLQNKLKPESQPSLRILKKANIRTVMVTGDNLLTAISVARDCGMVDEEDSVISVEAELLKHGDSKSPRIRVNYSHVKLPGFSEKLAVGKNGIIEDAC-IPLVQHSPYHLAMDGKTFNLIKKYDQVLLQKVVHKGTIFARMLPEQKLHLIEVLQLQGHQVGMCGDGANDCGALKTAHAGVSLSVAEASVASPFTAKKQNIECIPTLIREGRATLAATFGAFRYMVCYCFILLSAVLILFWDGEKPSDGAYVLIDIILNLLPPIVFGGTAAYPYLVKKLPTRSILSFVMQFSMISYILLQIGVLIFARYFLLMQPWFEPTVYNKETTLHPP-PSHMACTIISVNMMSYIIAAVIFAPGPPYRKHFFSN 1024          

HSP 2 Score: 109.383 bits (272), Expect = 3.012e-20
Identity = 59/164 (35.98%), Postives = 89/164 (54.27%), Query Frame = 0

Query:   18 VNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLKDKFEQIFEEDVVDHSSPEMTNNSSPICSFFINKKIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLGQLTVTDRHQEYGANLIKISVPPVLYLVFHE 181
            +N G+ DEM++ G+ F+  KT++T   VLL+GG  LIL++W+  I++   +S    K+A+K++L D + Q + E VV    PE++  S     +F NKKIKY+W      +VKL  +D        H    GL       R Q YG N I + V P+  L+F E
Sbjct:   11 LNPGEDDEMSITGYRFSFPKTILTCFAVLLSGGTLLILMSWKPSIRIKMTHSLCPFKQAEKVVLIDMYNQQYVEKVV---RPEISTTSPTQTIYFYNKKIKYIWQQDFLHFVKLGGLD-VESCEHVHSLARGLSSTEALLRLQLYGKNSILVEVKPIFKLIFQE 170          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. nr
Match: gi|1238885483|ref|XP_022255274.1| (probable cation-transporting ATPase 13A3 isoform X3 [Limulus polyphemus])

HSP 1 Score: 701.049 bits (1808), Expect = 0.000e+0
Identity = 362/792 (45.71%), Postives = 515/792 (65.03%), Query Frame = 0

Query:  187 SSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPDENNVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQLALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMA---TPTLVAPPKGSRNGSGNIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQG----LNVSYM----LEEN---EKSNIVHDSNFIKSVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVP--YKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTN 962
            SS  LVPGDVI LP +  VM ECDAIL+ GTCVV+ESMLTGES PITK+ I  ++N  ++    ++  +FCGTEIL   +   + +KA+V RTGF T KGELVR+ILFP P+ ++ +S+  KS+ +FL LG+  M Y+  +W  +G + +  ++  +D+ TFVVPP+LPA +T+ NA  Q+RL+   IFCL+S HI   GG+DVV FDKTGTLTE NLDLAG+  +++  FQ      +  +L S L+ A+ATCHSL  + G L G P+D+K+F  + WEL++        DY  +    P  +  PK        +   P   +IAI++ +PF+S +QRM+ +T K+GS  F++YIKGAPE ++  C   TIP +F   L+ YT+QG RV+A  ++SL  N  W E+    R DLE     +GL+++QN +K E+  +++ L +A+I TVMVTGDN+LTAISV RDC +V  + ++I VEAEL+  G S+     +N S++      E     K+ I+ D+  I  VQ   Y  A DGKTF LI+ +D+ LL ++V +G IFARMLPEQK+HLIE ++  G QV MCGDG NDCGALKTAHAG+SLS+AEASVA+PFT++  +I C+P LIREGRAT+ + F +F++ V +CF  L AVL++F+ G +PSD  Y+++DI L  LP I+ G    +  LVK+ P R +LSF+  FS+IS++  Q    +   +++ +Q WF P  Y  E  L PP PS+    I  ++  + ++AA+IF+ G PYRK  F+N
Sbjct:  242 SSHELVPGDVIFLPQHQCVM-ECDAILLSGTCVVSESMLTGESTPITKVPIAQDSNHPYSPLSNKRSTIFCGTEILHSCSSESSAVKALVYRTGFSTAKGELVRSILFPKPVQYKLYSELFKSMIIFLVLGIPAMVYTAIIWSHHGASAKNVVILVVDVATFVVPPLLPAVMTSINAHGQRRLRTHDIFCLNSGHINFAGGLDVVCFDKTGTLTEDNLDLAGIIPIQDVRFQHPFTPGSSHSLPSTLIHALATCHSLTVVDGRLMGYPVDIKMFNVVAWELEEP-----KADYHCSFERLPARIVRPKKKEAAFLRMSGMPVT-DIAIVRQFPFESLLQRMSVITCKEGSDNFELYIKGAPEMVSSFCDSNTIPENFLDVLELYTRQGFRVLALGYRSLEPNITWDEIFRKPRDDLECNLKLVGLLVLQNKLKPESQPSLRILKKANIRTVMVTGDNLLTAISVARDCGMVDEEDSVISVEAELLKHGDSKSPRIRVNYSHVKLPGFSEKLAVGKNGIIEDAC-IPLVQHSPYHLAMDGKTFNLIKKYDQVLLQKVVHKGTIFARMLPEQKLHLIEVLQLQGHQVGMCGDGANDCGALKTAHAGVSLSVAEASVASPFTAKKQNIECIPTLIREGRATLAATFGAFRYMVCYCFILLSAVLILFWDGEKPSDGAYVLIDIILNLLPPIVFGGTAAYPYLVKKLPTRSILSFVMQFSMISYILLQIGVLIFARYFLLMQPWFEPTVYNKETTLHPP-PSHMACTIISVNMMSYIIAAVIFAPGPPYRKHFFSN 1024          

HSP 2 Score: 109.383 bits (272), Expect = 2.453e-20
Identity = 59/164 (35.98%), Postives = 89/164 (54.27%), Query Frame = 0

Query:   18 VNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLKDKFEQIFEEDVVDHSSPEMTNNSSPICSFFINKKIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLGQLTVTDRHQEYGANLIKISVPPVLYLVFHE 181
            +N G+ DEM++ G+ F+  KT++T   VLL+GG  LIL++W+  I++   +S    K+A+K++L D + Q + E VV    PE++  S     +F NKKIKY+W      +VKL  +D        H    GL       R Q YG N I + V P+  L+F E
Sbjct:   11 LNPGEDDEMSITGYRFSFPKTILTCFAVLLSGGTLLILMSWKPSIRIKMTHSLCPFKQAEKVVLIDMYNQQYVEKVV---RPEISTTSPTQTIYFYNKKIKYIWQQDFLHFVKLGGLD-VESCEHVHSLARGLSSTEALLRLQLYGKNSILVEVKPIFKLIFQE 170          

BLAST of hypothetical protein DAPPUDRAFT_318475 vs. nr
Match: gi|1242799625|ref|XP_022340299.1| (probable cation-transporting ATPase 13A3 isoform X2 [Crassostrea virginica])

HSP 1 Score: 695.656 bits (1794), Expect = 0.000e+0
Identity = 417/1185 (35.19%), Postives = 631/1185 (53.25%), Query Frame = 0

Query:   18 VNKGQADEMTVEGFVFNPVKTLITVLVVLLTGGLFLILLTWRADIKLNCMYSKVELKEAQKILLKDKFEQIFEEDV-VDHS--------------------SPEMTNN---------SSP---------------------ICSFFINKKIKYVWDSGARTYVKLKNIDEFMKQADFHKCKEGLG----------------QLTVT---------------------------DRHQEYGANLIKISVPPVLYLVFHEAKTE-----------------------LSSMALVPGDVILLPTNGGVMMECDAILVEGTCVVNESMLTGESIPITKISIPD------ENNVQFAYDLQRQHVVFCGTEILQGKAQLGNFIKAVVIRTGFMTTKGELVRAILFPPPLDFQFHSDFLKSIYVFLTLGLIGMAYSLYMWISNGGTIQECLLNSLDILTFVVPPILPAALTANNAFAQKRLQKMGIFCLHSKHICLCGGIDVVAFDKTGTLTEGNLDLAGVCEVKNANFQETQADPTQL-ALDSRLVQAMATCHSLIKLKGELTGNPLDVKLFEAIEWELQDQHNMGVNPDYGMATPTLVAPPKGSRNGSG--NIKTAPSNLEIAILKTYPFDSAVQRMTTVTKKKGSQQFDVYIKGAPEKIAGTCKPETIPPDFASTLQWYTKQGLRVIAAAHKSLNANHRWKEVDDLSRSDLEQKADFLGLIIMQNLVKDETYGAIKELHEADINTVMVTGDNILTAISVGRDCELVKPDQTIIRVEAELIPDGYSQGL-NVSYMLEENEKSNIVHDSNFIK------SVQEKNYVFACDGKTFALIRNHDKALLDRIVQRGKIFARMLPEQKIHLIECMKDLGRQVIMCGDGCNDCGALKTAHAGISLSMAEASVAAPFTSRNVHIGCVPYLIREGRATMVSAFASFKFGVAFCFTQLIAVLMVFYIGTEPSDNQYLVVDIGLAALPIIMIGNCGPHNSLVKQKPMRHLLSFLPLFSVISFLFFQTLTYVGVWFYVQIQSWFVPYKFEAGLWPPNPSYEQTNIFLLSCSAAVVAAIIFSKGEPYRKPLFTNGIMAVWTVAAVAATSFMTLFESEDFAHRMNFKIPPSLEFRLVIVGVMVFNCIFCYLWEMYLIDGVLFAKILP-WYKLRIRGPHLPFEHLEEELRSKSGWPPIGDCKN 1068
            VN  Q D+M + G+  + ++ ++    +++T GL  +   W   + + C +SK  L EA+ ++L+D++ Q F   V ++H                     +P  +N          SSP                     +  +F+ KK+KY+W S ++T+ KL+ +D+  K + FH  K GL                 Q+ VT                           D +  Y + ++ ISV  +   ++   K +                       + S  LVPGD+I +P  G   M+CDA+L+ G C+VNESMLTGES+P+TK  +P+      + ++ F      +HV+FCGT+ILQ +      +KAVV+RTGF T KG+LVR+I++P P+DF+F+ D    + +   +  +G  Y++ + +      +E  L S+D++T  VPP LPAALT    FAQ RL+K  I+C+  + I + G I++V FDKTGTLTE  L+L  +  + +  F E   D  QL A  S L+ AMATCHSL  + G L+G+PLD+ +FEA  WEL++      +  Y M  PT+V P KGSRNG G  + +  P + EIAI++ + F S++QRM+ +T+K G+  F+++ KGAPE IA   KPET+P +F   L  YTK G RV+A A++SL A  ++ ++  + R  +E+   FLG ++M+N +K ET   I +L +A+I TVMVTGDN+LTA+SV R+C +V   + II V+A   P G  +    + YM  E+ +  +       K       V +  Y FA DG+++A+IR H   LL +   RG +FARM P+QK  LIE +++LG  V MCGDG NDCGALK AHAGISLS AEASVA+PFTS+  +I CVP LIR+GR  +V++F  FK+   +  TQ ++VL++++I    +D ++L +D+ L     +  G    ++ L K  P+  L S  P+ S+I  +  QT   V  +F VQ Q WFVPY  E        SYE   IFL+S    +  A++FSKG PYRK +F+N          V  + ++ ++  ED A     K+ PS+ +R V +G+ V N     L E Y+I+     + L  W +         +E  EEE+     WPP+   + 
Sbjct:   24 VNPAQEDQMEICGYRPSLIRKVMVWFFIIITAGLLRLFFYWLPHLFIKCTHSKCSLAEAEIVILRDQYLQWFVSRVKINHKNGMLGESAMKDGVAGITFSKTPFDSNTPLKMGDAQTSSPADPDLGGHVSEVDRVLELNRKLVRYFVTKKVKYIWSSESQTFKKLEGLDKNTKCSYFHATK-GLNYSQQQERMSMFGSNSIQIHVTPIVKLLFKEVLSPFYIFQLFSCGIWFADEYYYYASCIVVISVVSITATIYQTRKMQRTLRNTIESSTIVSRLCGEEYHDICSDDLVPGDIISIPRQG-CTMQCDAVLLTGNCIVNESMLTGESVPVTKTPLPNPRVSKSQEDILFNMKEHSKHVLFCGTQILQTRFYGSQQVKAVVLRTGFSTAKGDLVRSIMYPKPVDFKFNRDTYIFVGILAAIAGVGFIYTVVLMVQEHEAFEEIFLRSMDLVTIAVPPALPAALTIGIVFAQSRLKKSQIYCISPRSINVSGTINIVCFDKTGTLTEDGLNLQSIVPLHDGRFAEEVYDVKQLPAEKSLLLNAMATCHSLTIINGVLSGDPLDLIMFEATGWELEEPGEEEGS-RYDMIVPTVVRP-KGSRNGKGAFDQQFVPVSDEIAIMRQFTFASSLQRMSVITRKLGAPNFELFSKGAPEMIASLSKPETVPSNFHEVLIEYTKHGYRVLALAYRSLPAKLKYAKIQRIQREQVERSLTFLGFLVMENRLKPETTPIIHQLLDANIRTVMVTGDNMLTALSVARECGMVSEHKKIILVQA--FPGGSGKSEPTLEYMYTEDPQDRLQKSEKETKIPMPDGHVTDSPYHFAMDGRSWAIIRKHFPLLLQKFCVRGTVFARMSPDQKSQLIETLQELGYFVGMCGDGANDCGALKVAHAGISLSEAEASVASPFTSKQPNIECVPTLIRQGRGALVTSFGIFKYMACYSLTQFVSVLILYWIFANLTDFEFLYIDLFLLTSLSVTFGYTNAYDKLSKDPPLVSLFSISPVLSIILHMAIQTAGQVFCYFNVQEQPWFVPY--EVNEDDDYTSYENMAIFLISMYQYITLAVVFSKGRPYRKTIFSNYFFMGNLAVCVGMSLWLNIYPIEDVATFFELKVAPSIPYRCVYLGIAVINFFLDLLVETYVIESYFVQERLQCWIEDIFSTSKYQYEIQEEEMVRDQPWPPVSSAET 1200