vesamicol binding, snap_masked-scaffold22_size673200-processed-gene-5.22 (gene) Tigriopus kingsejongensis

Overview


Name	vesamicol binding
Unique Name	snap_masked-scaffold22_size673200-processed-gene-5.22
Type	gene
Organism	Tigriopus kingsejongensis (Tigriopus kingsejongensis)

Associated RNAi Experiments

Nothing found

Homology

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BLAST of vesamicol binding vs. L. salmonis genes
Match: EMLSAG00000012699 (supercontig:LSalAtl2s:LSalAtl2s964:5967:7469:-1 gene:EMLSAG00000012699 transcript:EMLSAT00000012699 description:"augustus_masked-LSalAtl2s964-processed-gene-0.1")

HSP 1 Score: 721.079 bits (1860), Expect = 0.000e+0
Identity = 352/513 (68.62%), Postives = 420/513 (81.87%), Query Frame = 0

Query:   47 MLYMVIVPIIPDYLRKIGVWETR----TIHNNNA------QTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQE--SKQMTNGYETKAYETTDMDEEMNYDEAPAPTNPFKAQQASNPFRK 547
            MLYMVIVPIIPDYLR IG WET      IH++N+      Q   +V++++N+TGNY++   N TR+++  GI+IEY+GED GIGVLFASKAA+QI INP+SG +IDK+GYDLPMMFGL +MFFSTLLFACGS+Y VLFFARSLQGVGSAFADTGG+SMIADRYTEE+ERSKA+GIALAFISFGCLVAPPFGG+LY + GKA+PFIILAF+CLIDG LLLLIMRP+KAKQ+EEG+V P  TP+W+L +D HIACCSGALI+ANVSLAFLEPTISKWM +TMDA+EWQ+G+IWLPA FPHV+GVI+TV+M  K+PEW++ALA IGL LEGLSCF+IPF TNYF+LMIPI  I FGIALIDT+LLP LG+IVDKKY SVYGSVYAIADISY AAYA+GPVIAGH+V  MGFTALNI VAI ++ Y+P LYYLKDMHDY PF+    GS  G+ + DPP K++Q   LQE  +K+M +  E K  E+   ++          TNPF+    +NPFRK
Sbjct:    1 MLYMVIVPIIPDYLRLIGAWETHQVPLNIHDHNSGPPPVQQRHEIVNFFSNSTGNYSITYVNTTRKLHFRGIMIEYEGEDSGIGVLFASKAAIQIFINPISGTIIDKVGYDLPMMFGLTIMFFSTLLFACGSSYGVLFFARSLQGVGSAFADTGGLSMIADRYTEESERSKALGIALAFISFGCLVAPPFGGVLYEFCGKAVPFIILAFICLIDGLLLLLIMRPMKAKQMEEGYVRPKGTPIWKLFMDPHIACCSGALIMANVSLAFLEPTISKWMTETMDAEEWQZGMIWLPALFPHVIGVIITVKMTGKFPEWIWALAAIGLALEGLSCFIIPFSTNYFVLMIPISIISFGIALIDTSLLPTLGFIVDKKYESVYGSVYAIADISYCAAYAIGPVIAGHVVEKMGFTALNIIVAILSIGYTPFLYYLKDMHDYKPFQ----GSEVGINVRDPPPKDFQAYQLQEVLNKKMIDSDEIKIEESEICEDT---------TNPFRTTNQNNPFRK 500

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BLAST of vesamicol binding vs. L. salmonis genes
Match: EMLSAG00000006135 (supercontig:LSalAtl2s:LSalAtl2s334:392742:405619:-1 gene:EMLSAG00000006135 transcript:EMLSAT00000006135 description:"augustus_masked-LSalAtl2s334-processed-gene-3.5")

HSP 1 Score: 307.76 bits (787), Expect = 5.530e-98
Identity = 177/491 (36.05%), Postives = 282/491 (57.43%), Query Frame = 0

Query:   16 ARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIH--------------NN-----------------NAQTSTLVSYYNNA-TGNYTVITTNMTRRINVGGIVIEYD-------GEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKD 467
            +R R   Y    +  R+L+L+ V+IALLLDNML   +VPIIP++L     +E R  H              N+                 +  T + + Y N    G       N++       + +E +        E++ +GV+FASKA VQ++ NP  G + ++IGY +PM  G ++MFFST++FA   +Y +LFFAR+LQG+GS+ +   GM M+A+R+ ++ ER  AMGIAL  ++ G L+ PPFGGI+Y + GK  PF++LA + L+DG L LL+++P   +  EE      A  L  L++D +I   +GA+  AN+ +A LEP++  +M D MD+++W+ G  +LPA   +++G  +   +  +   W  +   +GL++ G + F++PF T    L+IP+  I FGI ++D++++P LG++VD ++ SVYG VYAI DI++   YAVGP ++G IV   GF A+   + +    Y P L+ LKD
Sbjct:    9 SRTRIGAYFHNARESRKLILLIVAIALLLDNMLLTTVVPIIPEFL-----YEXRHRHPYLTPTPEPPPTTVNSVLDYDVYYGGGDPSIFVDTLTQSSIPYLNEXYAGEERRRQQNVSEMSEKERVALEKEIKHKDLVEENVEVGVMFASKAVVQLIANPFVGPLTNRIGYSIPMFAGFVIMFFSTIVFAFSRSYVILFFARALQGIGSSCSSVSGMGMLAERFPDDKERGNAMGIALGGLALGVLIGPPFGGIMYQFVGKTAPFLVLAMLALLDGCLQLLVLKPTITRTEEE------APSLKALIMDPYIIIAAGAITFANMGIAMLEPSLPIFMMDKMDSQKWEIGAAFLPASISYLIGTNIFGPLGHRMGRWKASF--VGLIVIGFALFLVPFATVPGHLVIPMGAIGFGIGMVDSSMMPELGFLVDIRHSSVYGGVYAIGDIAFCLGYAVGPALSGSIVREYGFKAMLFGIGLLCFIYGPFLFLLKD 486

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BLAST of vesamicol binding vs. L. salmonis genes
Match: EMLSAG00000009550 (supercontig:LSalAtl2s:LSalAtl2s615:180941:184069:1 gene:EMLSAG00000009550 transcript:EMLSAT00000009550 description:"maker-LSalAtl2s615-snap-gene-1.20")

HSP 1 Score: 177.948 bits (450), Expect = 3.981e-50
Identity = 120/371 (32.35%), Postives = 186/371 (50.13%), Query Frame = 0

Query:  115 EDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWA-GKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHP--------NATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVI----LTVRMARKYPEWVYALAGIGLV-LEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYV-SVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHD 470
            E+  IG LFASKA VQ++ N + G +   IG  LP   G I + FS+L++   S Y  L  ARS+ G+GS+     G+ MIA  Y ++ ERSK M   +  ++ G L+    GG LY  A  K LPF +++FV      LL+  M  L     E   +HP                L + ++    G++ +A  S+  LEPT+  W+ D M  ++WQ G+ ++     +++G      ++ R+ RK         G+G + L G+    IP  T+  LL IP   I  GI LID+++LP+L  I D+      YG VYAIA+ + S AY + P+I    V   GF ++   + +  + Y P L++L +  +
Sbjct:   89 ENGRIGYLFASKALVQLVTNFLIGKMTTLIGXRLPFTIGSIFLLFSSLIYTLSSRYIWLLLARSVHGIGSSCVCVSGIGMIASNYGDDEERSKIMSHTMGGVAIGILIGYSMGGFLYYLAQSKLLPFALISFV------LLIFTMWQLLLYSREASSLHPFGMESRSEKGPSTLEFLQNRNVLIVVGSIWIATSSVGILEPTLPLWIMDRMHPRKWQLGITFVSDSIGYLIGTHFFAGVSYRVGRKK-------IGVGAICLVGIGSISIPHATSLPLLTIPQFMIGLGIGLIDSSMLPLLAKIXDQTSSGEAYGVVYAIAETAVSFAYGISPLIGSLSVEMFGFCSVFTLLGLMNMTYVPILFFLDENRE 446

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BLAST of vesamicol binding vs. L. salmonis genes
Match: EMLSAG00000007792 (supercontig:LSalAtl2s:LSalAtl2s457:18904:31426:-1 gene:EMLSAG00000007792 transcript:EMLSAT00000007792 description:"maker-LSalAtl2s457-augustus-gene-0.26")

HSP 1 Score: 51.9878 bits (123), Expect = 3.879e-7
Identity = 73/303 (24.09%), Postives = 121/303 (39.93%), Query Frame = 0

Query:   98 NMTRRINVGGIV----IEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGIL------YNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHP-NATPLWRLLID--------------SHIACCSGAL--------IVANVSLAFLE--PTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGL 365
            N   R  V GI+     EYD  D   G L  +     ++  P+ G + D+    L M  G+ +    TL+ +    Y    F R + G+G A   T   ++I+D + +E ERSK + +    I  GC      G  +      + W  +  P +  A V +I    L ++  P + +   EGH +    T  ++ L D              + +A C+GAL        I A  S    E  P     +     A     G++ +P      +G IL+ ++ RK+P     + G+GLV+  +
Sbjct:  681 NYMDRFTVAGILENIKDEYDIADAKAGALQTAFVITYMIFAPLFGYLGDRHSRRLIMAIGVFIWAIFTLIGSFMPGYYSFLFCRGMVGIGEASYSTIAPTIISDMFVKE-ERSKMLAVFFFAIPVGCGSGYMLGSEMSHLTGDWRWGLRVTPILGFAAVVMI----LFMLKDPNRGEC--EGHGNEIKTTTYFQDLKDLAANKSFVLSTFGFTCVAFCTGALSWWGPLYIISAIKSFGNPEHYPMPVNNVALVFGAITMLSGIVGVP------LGSILSTKLQRKFPRADPIICGLGLVISAI 970

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BLAST of vesamicol binding vs. SwissProt
Match: gi|34924873|sp|O17444.2|VACHT_DROME (RecName: Full=Vesicular acetylcholine transporter; Short=VAChT)

HSP 1 Score: 589.341 bits (1518), Expect = 0.000e+0
Identity = 294/513 (57.31%), Postives = 374/513 (72.90%), Query Frame = 0

Query:    1 MPDFDLPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTNGYETK 513
            M  F +P + +++ E +   W+ +QEP  QRRL+LV VSIALLLDNMLYMVIVPIIPDYLR+IG ++            T     +N TG    +               ++ G+D   G+LFASKA VQ+++NP SG +IDKIGYDLPMM GL +MFFST +FACGS+YSVLFFARSLQG GSAFADT G++MIADR+TEE ERS+A+GIALAFISFGCLVAPPFGG LY +AGK +PF+ILA VCL+DG +LLL+M+P+K    +   V     P+WRLL+D +IA C+GAL ++NV+LAFLEPTIS WM D M    W+ G++WLPAFFPHV+GV++TV+MARKYP+  + +A  GL LEG SCF+IPFC+ Y +LM+PIC ICFGIALIDTALLP LGY+VD +YVSVYGS+YAIADISYS AYAVGP+IAG +V ++GFTALN  +A   LAY P L  L++++D+ PFE     +   + + DPP KEYQT  + + K +  G +  
Sbjct:    1 MASFQIPVINLEVREVKDIVWEKIQEPVNQRRLILVIVSIALLLDNMLYMVIVPIIPDYLREIGSFD---------DGPTPPPLRDNITGKIIPVHH-------------DHHGQDSATGILFASKAIVQLMVNPFSGGLIDKIGYDLPMMIGLTIMFFSTAVFACGSSYSVLFFARSLQGAGSAFADTAGLAMIADRFTEENERSQALGIALAFISFGCLVAPPFGGALYQFAGKEVPFLILALVCLLDGLMLLLVMKPVKEAMKQSKDVQDQVIPIWRLLMDPYIAVCAGALTMSNVALAFLEPTISLWMEDNMTTDNWKIGMVWLPAFFPHVLGVVITVKMARKYPQHQWLMAAGGLALEGFSCFIIPFCSGYKMLMLPICVICFGIALIDTALLPTLGYLVDVRYVSVYGSIYAIADISYSIAYAVGPIIAGGVVEAIGFTALNFLIAFSNLAYVPVLRKLRNIYDFKPFE-----NEANILMQDPPNKEYQTYVMHDQKPVEGGVKNH 486

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BLAST of vesamicol binding vs. SwissProt
Match: gi|464998|sp|P34711.1|UNC17_CAEEL (RecName: Full=Vesicular acetylcholine transporter unc-17; AltName: Full=Uncoordinated protein 17)

HSP 1 Score: 504.982 bits (1299), Expect = 5.162e-174
Identity = 272/546 (49.82%), Postives = 353/546 (64.65%), Query Frame = 0

Query:    4 FDLPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTM-DAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGS----------GGGVTIGDPP---TKEYQTIALQESKQMTNGYETKAYETTDMDEEMNYDEAPAPTNP 535
            F++P +       +     +L++   Q++ VLV VSIALLLDNMLYMVIVPIIP YLR I  ++  T    + +TS L      A G Y V          VGG  I +  E++ +G LFASKA +QI +NP SG +ID++GY++PM+ GL  MFFST +FA G +Y VL FARSLQG GSAFADT G++MIADR+TEE ERS A+GIALAFISFGCLVAPPFG +LY+ AGK +PF+IL+FVCL D   + +++ P +      G      TP+WRL +D  IACCSGALI+ANVSLAFLEPTI+ WM + M D   W  G+IWLP FFPHV+GV +TV+M R +P   +A+A +GL +EG++CF IP+ T+   L+IP+ F+CFGIALIDT+LLPMLG++VD ++VSVYGSVYAIADISYS AYA GP+IAG IV + GFTALNI +    + Y+P L+ L+ +H Y+      D +           GG   G P     + YQ    Q+S Q        A    D   +  +     P NP
Sbjct:    3 FNVPVINRDSEILKADAKKWLEQQDNQKKCVLVIVSIALLLDNMLYMVIVPIIPKYLRDIHNYQV-TFEGYHNETSQL------ANGTYLVR--------EVGG-RINFLDEELELGWLFASKALLQIFVNPFSGYIIDRVGYEIPMILGLCTMFFSTAIFALGKSYGVLLFARSLQGFGSAFADTSGLAMIADRFTEENERSAALGIALAFISFGCLVAPPFGSVLYSLAGKPVPFLILSFVCLADAIAVFMVINPHRRGTDSHGE-KVQGTPMWRLFMDPFIACCSGALIMANVSLAFLEPTITTWMSEMMPDTPGWLVGVIWLPPFFPHVLGVYVTVKMLRAFPHHTWAIAMVGLAMEGIACFAIPYTTSVMQLVIPLSFVCFGIALIDTSLLPMLGHLVDTRHVSVYGSVYAIADISYSLAYAFGPIIAGWIVTNWGFTALNIIIFATNVTYAPVLFLLRKVHSYDTLGAKGDTAEMTQLNSSAPAGGYN-GKPEATTAESYQGWEDQQSYQNQAQIPNHAVSFQDSRPQAEFPAGYDPLNP 530

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BLAST of vesamicol binding vs. SwissProt
Match: gi|34924880|sp|P81721.2|VACHT_TORCA (RecName: Full=Vesicular acetylcholine transporter; Short=VAChT; AltName: Full=TorVAChT)

HSP 1 Score: 494.197 bits (1271), Expect = 5.677e-170
Identity = 247/493 (50.10%), Postives = 342/493 (69.37%), Query Frame = 0

Query:   13 LSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHN-NNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESK 504
            + E  +R    + EP+ +R+++LV V IA+LLDNMLYMVIVPIIP+YL  I  ++   I   +N    +L++    A          + R  N          ED+ IGVLFASKA +Q+L NP +G  ID++GYD+P++ GL +MFFST+ FA G +Y+VLF ARSLQG+GSAFADT G++MIAD+YTEE+ER++A+GIALAFISFG LVAPPFGG+LY +AGK +PF++L+FVCL+DG LLL+++ P  ++  E        TP+++L+ID +IA  +GAL   N+ LAFLEPTIS WM  TM+A EWQ G+ WLPAFFPH++GV +TV++A KYP + +    +GLV+ G S   IP C N+  L+IP+C +CFGIAL+DTALLP L ++VD +YVSVYGSVYAIADISYS AYA+GP++AG IV+ +GF  LN+ + +  + Y+P L +L+++    P       S   + + + P   Y TI ++E K
Sbjct:   21 IGERSKRISGAMNEPRRKRKILLVIVCIAMLLDNMLYMVIVPIIPNYLETIRTYKLVYITTPSNGTNGSLLNSTQRAV---------LERNPNAN--------EDIQIGVLFASKAILQLLSNPFTGTFIDRVGYDIPLLIGLTIMFFSTITFAFGESYAVLFAARSLQGLGSAFADTSGIAMIADKYTEESERTQALGIALAFISFGSLVAPPFGGVLYQFAGKWVPFLVLSFVCLLDGILLLMVVTPFASRTREN---MLQGTPIYKLMIDPYIAVVAGALTTCNIPLAFLEPTISNWMKKTMNASEWQMGITWLPAFFPHILGVYITVKLAAKYPNYQWFYGAVGLVIIGASSCTIPACRNFEELIIPLCALCFGIALVDTALLPTLAFLVDIRYVSVYGSVYAIADISYSVAYALGPIMAGQIVHDLGFVQLNLGMGLVNILYAPALLFLRNVCQMKP-----SLSERNILLEEGPKGLYDTIIMEERK 488

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BLAST of vesamicol binding vs. SwissProt
Match: gi|34924981|sp|Q91498.2|VACHT_TORMA (RecName: Full=Vesicular acetylcholine transporter; Short=VAChT; AltName: Full=Vesamicol-binding protein)

HSP 1 Score: 492.656 bits (1267), Expect = 2.108e-169
Identity = 247/504 (49.01%), Postives = 347/504 (68.85%), Query Frame = 0

Query:   13 LSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHN-NNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHP-NATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTNGYETKA 514
            + E  +R    + EP+ +R+++LV V IA+LLDNMLYMVIVPIIP+YL  I  ++   I   +N    +L++    A          + R  N          ED+ IGVLFASKA +Q+L NP +G  ID++GYD+P++ GL +MFFST+ FA G +Y++LF ARSLQG+GSAFADT G++MIAD+YTEE+ER++A+GIALAFISFG LVAPPFGG+LY +AGK +PF++L+FVCL+DG LLL+++ P  ++      V+    TP+++L+ID +IA  +GAL   N+ LAFLEPTIS WM  TM+A EWQ G+ WLPAFFPH++GV +TV++A KYP + +    +GLV+ G S   IP C N+  L+IP+C +CFGIAL+DTALLP L ++VD +YVSVYGSVYAIADISYS AYA+GP++AG IV+ +GF  LN+ + +  + Y+P L +L+++    P       S   + + + P   Y TI ++E K+    + T +
Sbjct:   17 IGERSKRISGAMNEPRRKRKILLVIVCIAMLLDNMLYMVIVPIIPNYLETIRTYKLVYITTPSNGTNGSLLNSTQRAV---------LERNPNAN--------EDIQIGVLFASKAILQLLSNPFTGTFIDRVGYDIPLLIGLTIMFFSTITFAFGESYAILFAARSLQGLGSAFADTSGIAMIADKYTEESERTQALGIALAFISFGSLVAPPFGGVLYQFAGKWVPFLVLSFVCLLDGILLLMVVTPFASRT----RVNTLQGTPIYKLMIDPYIAVVAGALTTCNIPLAFLEPTISNWMKKTMNASEWQMGITWLPAFFPHILGVYITVKLAAKYPNYQWLYGAVGLVIIGASSCTIPACRNFEELIIPLCALCFGIALVDTALLPTLAFLVDIRYVSVYGSVYAIADISYSVAYALGPIMAGQIVHDLGFVQLNLGMGLVNILYAPGLLFLRNVCQMKP-----SLSERNILLEEGPKGLYDTIIMEERKEAKEPHGTSS 494

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BLAST of vesamicol binding vs. SwissProt
Match: gi|34924984|sp|Q91514.1|VACHT_TORTO (RecName: Full=Vesicular acetylcholine transporter; Short=VAChT; AltName: Full=Vesamicol-binding protein)

HSP 1 Score: 490.73 bits (1262), Expect = 1.101e-168
Identity = 251/507 (49.51%), Postives = 347/507 (68.44%), Query Frame = 0

Query:   13 LSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETR--TIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYD---GEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTNGYETKA 514
            + E  +R    + EP  +R+++LV V IA+LLDNMLYMVIVPI+P+YL  I  ++    TI +N    S L                N T+R      V+E +    ED+ IGVLFASKA +Q+L NP +G  ID++GYD+P++ GL +MFFST+ FA G +Y++LF ARSLQG+GSAFADT G++MIAD+YTEE+ER++A+GIALAFISFG LVAPPFGG+LY +AGK +PF++L+FVCL+DG LLL+++ P  ++    G+     TP+ +L+ID +IA  +GAL   N+ LAFLEPTIS WM  TM+A EWQ G+ WLPAFFPH++GV +TV++A KYP + +     GLV+ G+S   IP C N+  L+IP+C +CFGIAL+DTALLP L ++VD +YVSVYGSVYAIADISYS AYA+GP++AG IV+ +GF  LN+ + +  + Y+P L +L+++    P       S   + + D P   Y TI ++E K     + T +
Sbjct:   17 IGERSKRISGAMNEPLRKRKILLVIVCIAMLLDNMLYMVIVPIVPNYLETIRTYKLVYITIPSNGTNGSLL----------------NSTQRA-----VLERNPNANEDIQIGVLFASKAILQLLSNPFTGTFIDRVGYDIPLLIGLTIMFFSTITFAFGESYAILFAARSLQGLGSAFADTSGIAMIADKYTEESERTQALGIALAFISFGSLVAPPFGGVLYQFAGKWVPFLVLSFVCLLDGILLLMVVTPFASRT--RGNTL-QGTPIHKLMIDPYIAVVAGALTTCNIPLAFLEPTISNWMKKTMNASEWQMGITWLPAFFPHILGVYITVKLAAKYPNYQWLYGAFGLVIIGVSSCTIPACRNFEELIIPLCALCFGIALVDTALLPTLAFLVDIRYVSVYGSVYAIADISYSVAYALGPIMAGQIVHDLGFVQLNLGMGLVNILYAPALLFLRNVCQMKP-----SLSERNILLEDGPKGLYDTIIMEERKAAKEPHGTSS 494

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BLAST of vesamicol binding vs. SwissProt
Match: gi|123884384|sp|Q08C75.1|VACHA_DANRE (RecName: Full=Probable vesicular acetylcholine transporter-A; Short=VAChT-A; AltName: Full=Solute carrier family 18 member 3-A)

HSP 1 Score: 484.567 bits (1246), Expect = 2.990e-166
Identity = 249/538 (46.28%), Postives = 351/538 (65.24%), Query Frame = 0

Query:   10 GMKLSEARQRC---WDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTNGYETKAYETTDMDEEMNYDEAPAPTNPFKAQQASNP 544
             +KLSE  +R     + +Q+P+ QRR++LV V +ALLLDNMLYMVIVPI+PDYL  +   E+   H                 GN ++   N+T+  N           D+ IGVLFASKA +Q+L+NP++G  ID++GYD+P++ GL +MF ST +FA    Y+ LF ARSLQG+GSAFADT G++MIAD+Y EE+ERS+A+GIALAFISFG L APPFGG+LY +AGK  PFI LA VCL DG L L +++P  ++  E     P  TP+++L+ID +IA  +GAL   N+ LAFLEPTI+ WM +TM+A +WQ G+ WLPAFFPH++GV LTV++A KYP   +    +G+V+ G S  ++P C N+  L+IP+C +CFGIAL+DTALLP L ++VD ++VSVYGSVYAIADISY  AYA+GP++AG IV+ +GF  LN+ + +  + Y+P L  L+++    P       S   + + +  T  Y TI ++E ++  +GY +       +DE   +      +  F  ++ S P
Sbjct:   14 AVKLSEMGERTKQLGNAIQDPERQRRIILVIVCVALLLDNMLYMVIVPIVPDYLAHLES-ESEQAH---------------VKGNSSI---NITQNENF----------DLQIGVLFASKAILQLLVNPLTGTFIDRVGYDIPLLIGLSIMFVSTCIFAFAENYATLFMARSLQGLGSAFADTSGIAMIADKYAEESERSRALGIALAFISFGSLAAPPFGGVLYEFAGKRFPFIALACVCLADGILCLTVLKPFSSRTREN---MPVGTPIYKLMIDPYIAVVAGALTTCNIPLAFLEPTIANWMEETMNASQWQIGITWLPAFFPHILGVYLTVKLAAKYPHLQWFYGALGMVIIGASSCIVPACKNFEQLIIPLCGVCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYCVAYALGPIVAGKIVHDLGFVQLNLGMGLANVLYAPALLLLRNVSLMKP-----SHSERNMLLEEGATGLYDTIRMEERQRKKHGYSSSG-NCVPIDENGTF---AGQSKSFSEEETSEP 510

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BLAST of vesamicol binding vs. SwissProt
Match: gi|34924877|sp|P59845.1|VACHB_DANRE (RecName: Full=Probable vesicular acetylcholine transporter-B; Short=VAChT-B; AltName: Full=Solute carrier family 18 member 3-B)

HSP 1 Score: 460.299 bits (1183), Expect = 4.523e-157
Identity = 250/503 (49.70%), Postives = 329/503 (65.41%), Query Frame = 0

Query:   13 LSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNP-FEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTNGYETKA 514
            + E  +     L+EP+ +RRL+LV V +ALLLDNMLYMVIVPIIPDYL                  + L     N++ +                        D+ IGVLFASKA +Q+L+NP+SG  ID++GYDLP++ GL+VMF ST +FA    Y  LF ARSLQG+GSAFADT G++MIAD++TEEAERS+A+GIALAFISFG LVAPPFGGILY +AGK +PFI+LA VCL DG LLL +++P   +  E     P  TP+ RL++D +IA  +GAL V N+ LAFLEPTI+ WM  TMDA +WQ GL+WLPAF PHV+GV +TVR+A +YPE  +    +G+V+ G S   +P C  +  L+ P+C ICFGIAL+DTALLP L ++VD ++VSVYGSVYAIADISYS AYA+GPV+AG IV+++GF  LN+ + +  + Y+P L  L+ +    P F E        V + + P+  Y +I L+E      G    A
Sbjct:   20 MGERSRELGGALREPERKRRLLLVVVCVALLLDNMLYMVIVPIIPDYL------------------ADLRGERGNSSADL-----------------------DIQIGVLFASKALLQLLVNPLSGTFIDRVGYDLPLLIGLLVMFLSTCIFAFAENYGTLFAARSLQGLGSAFADTSGIAMIADKFTEEAERSRALGIALAFISFGSLVAPPFGGILYEFAGKRVPFIVLACVCLADGVLLLTVVKPFSDRTREN---MPVGTPIHRLMVDPYIAVVAGALTVCNIPLAFLEPTIANWMESTMDASKWQMGLVWLPAFLPHVLGVYITVRLAARYPERQWFYGALGMVIIGASSCTVPACKTFGELVFPLCGICFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYAMGPVVAGQIVHNLGFVQLNLGMGLVNVLYAPALLLLRPVCQIKPSFSE------RNVLLEEGPSGLYDSIRLEERALRRKGLSAGA 472

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BLAST of vesamicol binding vs. SwissProt
Match: gi|34924861|sp|Q62666.2|VACHT_RAT (RecName: Full=Vesicular acetylcholine transporter; Short=VAChT; Short=rVAT; AltName: Full=Solute carrier family 18 member 3)

HSP 1 Score: 457.603 bits (1176), Expect = 2.206e-155
Identity = 237/444 (53.38%), Postives = 317/444 (71.40%), Query Frame = 0

Query:   12 KLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKI-GVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIV---IEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYP--EWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNI 449
            KLSEA       LQEP+ QRRLVLV V +ALLLDNMLYMVIVPI+PDY+  + G  E  T+ +   + +       NA+      + + T   +   I+      + ED+ IGVLFASKA +Q+L+NP+SG  ID++ YD+P++ GL VMF ST++FA    Y+ LF ARSLQG+GSAFADT G++MIAD+Y EE ERS+A+G+ALAFISFG LVAPPFGGILY +AGK +PF++LA V L D  LLL + +P  A      ++ P  TP+ RL++D +IA  +GAL   N+ LAFLEPTI+ WM  TM A EW+ G++WLPAF PHV+GV LTVR+A +YP  +W+Y    +GL + G+S  V+P C ++  L++ +C +CFGIAL+DTALLP L ++VD ++VSVYGSVYAIADISYS AYA+GP++AGHIV+S+GF  L++
Sbjct:   16 KLSEA---VGAALQEPQRQRRLVLVIVCVALLLDNMLYMVIVPIVPDYIAHMRGGSEGPTLVSEVWEPTLPPPTLANASAYLANTSASPTAAGSARSILRPRYPTESEDVKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLGVMFASTVMFAFAEDYATLFAARSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVALAFISFGSLVAPPFGGILYEFAGKRVPFLVLAAVSLFDALLLLAVAKPFSAAARARANL-PVGTPIHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMVWLPAFVPHVLGVYLTVRLAARYPHLQWLY--GALGLAVIGVSSCVVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQLSL 453

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BLAST of vesamicol binding vs. SwissProt
Match: gi|34924876|sp|O35304.1|VACHT_MOUSE (RecName: Full=Vesicular acetylcholine transporter; Short=VAChT; AltName: Full=Solute carrier family 18 member 3)

HSP 1 Score: 457.603 bits (1176), Expect = 2.432e-155
Identity = 236/444 (53.15%), Postives = 317/444 (71.40%), Query Frame = 0

Query:   12 KLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKI-GVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIV---IEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYP--EWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNI 449
            KLSEA       LQEP+ QRRLVLV V +ALLLDNMLYMVIVPI+PDY+  + G  E+ T+ +   + +       NA+      + + T   +   I+      + ED+ IGVLFASKA +Q+L+NP+SG  ID++ YD+P++ GL VMF ST++FA    Y+  F ARSLQG+GSAFADT G++MIAD+Y EE ERS+A+G+ALAFISFG LVAPPFGGILY +AGK +PF++LA V L D  LLL + +P  A      ++ P  TP+ RL++D +IA  +GAL   N+ LAFLEPTI+ WM  TM A EW+ G++WLPAF PHV+GV LTVR+A +YP  +W+Y    +GL + G+S  V+P C ++  L++ +C +CFGIAL+DTALLP L ++VD ++VSVYGSVYAIADISYS AYA+GP++AGHIV+S+GF  L++
Sbjct:   16 KLSEA---VGAALQEPQRQRRLVLVIVCVALLLDNMLYMVIVPIVPDYIAHMRGGSESPTLISEVWEPTLPPPTLANASAYLANTSASPTAAGSARSILRPRYPTESEDVKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLGVMFASTVMFAFAEDYATFFAARSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVALAFISFGSLVAPPFGGILYEFAGKRVPFLVLAAVSLFDALLLLAVAKPFSAAARARANL-PVGTPIHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMVWLPAFVPHVLGVYLTVRLAARYPHLQWLY--GALGLAVIGVSSCVVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQLSL 453

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BLAST of vesamicol binding vs. SwissProt
Match: gi|313104043|sp|Q16572.2|VACHT_HUMAN (RecName: Full=Vesicular acetylcholine transporter; Short=VAChT; AltName: Full=Solute carrier family 18 member 3)

HSP 1 Score: 456.062 bits (1172), Expect = 8.665e-155
Identity = 241/447 (53.91%), Postives = 311/447 (69.57%), Query Frame = 0

Query:   12 KLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDY---LRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIE----YDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYP--EWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNI 449
            KLSEA       LQEP+ QRRLVLV V +ALLLDNMLYMVIVPI+PDY   +R  G   TRT         TL          YT  T+         G  +      + ED+ IGVLFASKA +Q+L+NP+SG  ID++ YD+P++ GL VMF ST+LFA    Y+ LF ARSLQG+GSAFADT G++MIAD+Y EE ERS+A+G+ALAFISFG LVAPPFGGILY +AGK +PF++LA V L D  LLL + +P  A      ++ P  TP+ RL++D +IA  +GAL   N+ LAFLEPTI+ WM  TM A EW+ G+ WLPAF PHV+GV LTVR+A +YP  +W+Y    +GL + G S  ++P C ++  L++ +C +CFGIAL+DTALLP L ++VD ++VSVYGSVYAIADISYS AYA+GP++AGHIV+S+GF  L++
Sbjct:   16 KLSEAVGAA---LQEPRRQRRLVLVIVCVALLLDNMLYMVIVPIVPDYIAHMRGGGEGPTRT---PEVWEPTLPLPTPANASAYTANTSASPTAAWPAGSALRPRYPTESEDVKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLGVMFASTVLFAFAEDYATLFAARSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVALAFISFGSLVAPPFGGILYEFAGKRVPFLVLAAVSLFDALLLLAVAKPFSAAARARANL-PVGTPIHRLMLDPYIAVVAGALTTCNIPLAFLEPTIATWMKHTMAASEWEMGMAWLPAFVPHVLGVYLTVRLAARYPHLQWLY--GALGLAVIGASSCIVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVYAIADISYSVAYALGPIVAGHIVHSLGFEQLSL 453

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BLAST of vesamicol binding vs. nr
Match: gi|1325327285|ref|XP_023345498.1| (vesicular acetylcholine transporter-like isoform X1 [Eurytemora affinis] >gi|1325327287|ref|XP_023345499.1| vesicular acetylcholine transporter-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 789.26 bits (2037), Expect = 0.000e+0
Identity = 384/550 (69.82%), Postives = 446/550 (81.09%), Query Frame = 0

Query:    3 DFDLPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTN-----GYETKAYETTDMDEEMNYDEAPAPTNPFKAQQASNPFRK 547
            D  +PF+G  L + RQ  WD LQ+P+ QR+LVLVTVSIALLLDNMLYMVIVPIIPDYLR IG W T    +   Q   ++ YY N+TGNY++   N T +INV GI+IEY+GED GIG+LFASKAAVQI INP+SG VID+IGYDLPMMFGL VMFFSTLLFACG++Y VLFFARSLQGVGSAFADTGG++M+ADRYTEEAERSKA+GIALAFISFGCLVAPPFGG LY + GK +PFIILAFVCL DGFLLLLIMRP+K K+ E+G   P ATP+WRLLID HIACC+GAL+ AN+ LAFLEPTISKWM DTM+A+EWQQGLIWLPAFFPHV GV+LTV+MARKYPE+ + LA +GL LEG+SCF+IPF  NYF+LM PIC ICFGIAL+DTALLP LG+IVDKKY SVYGSVYAIADISYSAAYA GPV AGHIV +MGFTALNI VAI ++ Y+P +YYLKDMHDYN +EE   G+   V +GDPPTKEYQT  L E K + N     G +T+ Y   +  EE N++E     NPF+  Q +NPFR+
Sbjct:    5 DMTVPFVGKSLKDVRQEWWDALQQPEYQRKLVLVTVSIALLLDNMLYMVIVPIIPDYLRYIGAWNTHET-STPGQVQEVIKYYANSTGNYSITYINKTSKINVNGILIEYEGEDSGIGLLFASKAAVQIFINPISGTVIDRIGYDLPMMFGLTVMFFSTLLFACGTSYGVLFFARSLQGVGSAFADTGGLAMVADRYTEEAERSKALGIALAFISFGCLVAPPFGGFLYEFCGKPVPFIILAFVCLFDGFLLLLIMRPMKHKEAEDGKEIPVATPMWRLLIDPHIACCAGALVSANICLAFLEPTISKWMNDTMNAEEWQQGLIWLPAFFPHVAGVVLTVKMARKYPEYQWLLAMVGLFLEGVSCFIIPFSQNYFVLMFPICIICFGIALVDTALLPTLGFIVDKKYTSVYGSVYAIADISYSAAYAFGPVAAGHIVETMGFTALNIIVAIISIGYAPIIYYLKDMHDYNKYEE--PGTEENVLMGDPPTKEYQTYNLAEGKPVLNGAPGPGTQTEYYAQVE-QEETNFNEN-ITANPFRQPQGANPFRR 549

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BLAST of vesamicol binding vs. nr
Match: gi|1022766571|gb|KZS11615.1| (Vesicular acetylcholine transporter [Daphnia magna])

HSP 1 Score: 636.721 bits (1641), Expect = 0.000e+0
Identity = 318/530 (60.00%), Postives = 397/530 (74.91%), Query Frame = 0

Query:    6 LPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPN---------ATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDG-------------------SGGGVTI----GDPPTKEYQTIALQES 503
            +PF+ M+LS A++   + ++EP  QRRLVLV VSIALLLDNMLYMVIVPIIPDYLR IG W+T           + V ++ N TGNY+V   N T    V G+V+ Y+GED  IGVLFASKA VQ++INP SG +ID++GY+LPMM GL +MFFST +FACG +Y VLFFARSLQGVGSAFADT G++MIADR+TEE+ER+KA+GIALAFISFGCLVAPPFGG+LY +AGK LPF+IL+ VCL+D  +L L+MRP    Q+ +     N          TP+WRLL+D +IA CSGAL++ANVSLAFLEPTIS WM DTM+ ++WQ G+IWLPAFFPHV+GV+LTVRMA +YP++ + +A  GL LEGL CF+IPF T+Y++LMIPIC ICFGIALIDTALLP +GY+VD +YVSVYGS+YAIADISYS AYA+GP+IAG++V+S+GF ALNI +A+ TL + P L  L+ +++Y PF    DG                   +GGG  I     DPP K+YQT ALQES
Sbjct:    4 IPFLNMELSLAKEIVMEKIREPHNQRRLVLVIVSIALLLDNMLYMVIVPIIPDYLRTIGAWDTHIEGGEYVTEPSSVRWHVNTTGNYSV-PGNKTSLHLVNGVVV-YEGEDAAIGVLFASKAIVQLMINPFSGALIDRVGYELPMMIGLTIMFFSTAVFACGRSYGVLFFARSLQGVGSAFADTSGLAMIADRFTEESERTKALGIALAFISFGCLVAPPFGGLLYEFAGKELPFLILSLVCLVDALMLKLVMRPRGLSQLGKSTSAVNLAGAQGPQVGTPIWRLLMDPYIAVCSGALMMANVSLAFLEPTISVWMMDTMNVEQWQLGMIWLPAFFPHVLGVVLTVRMAARYPQYTWVMAAFGLALEGLCCFIIPFATSYWVLMIPICGICFGIALIDTALLPTMGYVVDVRYVSVYGSIYAIADISYSFAYAIGPIIAGNVVDSIGFLALNIGIAVSTLLFCPVLMLLRHIYEYKPFGANADGEEMTGLGPAPTIGHGFSGIAGGGSNIIPLMSDPPDKQYQTYALQES 531

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BLAST of vesamicol binding vs. nr
Match: gi|943934311|ref|XP_014357236.1| (PREDICTED: vesicular acetylcholine transporter [Papilio machaon] >gi|930680483|gb|KPJ20169.1| Vesicular acetylcholine transporter [Papilio machaon])

HSP 1 Score: 624.394 bits (1609), Expect = 0.000e+0
Identity = 303/520 (58.27%), Postives = 392/520 (75.38%), Query Frame = 0

Query:    6 LPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTNGYETKAYETTDMDEEMN 525
            +P + +++ E R+  W+ ++EP +QR+++LV VSIALLLDNMLYMVIVPIIPDYLR IG W         A    +V+      GN T+I T +        I   ++G+D   G+LFASKA VQ++INP SG +ID+IGYD+PMM GLI+MF ST +FACG +Y +LFFARSLQGVGSAFADT G++MIADR+TEEAERSKA+GIALAFISFGCLVAPPFGG LY +AGK +PF+ILA + L+DGF+LLL+M+PLK +  E     PN TP+W+LL+D +IA C+GAL+++NV+LAFLEPTIS WM D +  + W+ G+IWLPAFFPHV+GVI+TV+MARKYP+  + +A  GL LEGL CF+IPF T+Y +LMIPIC ICFGIALIDTALLP LGY+VD +YVSVYGS+YAIADISYS AYAVGP+IAG +V  +GFTALN+F+A   L Y+P L YL+ ++D+ PFE     +   + + DPP KEYQT ++Q+ K + NG      E ++M  ++ 
Sbjct:   17 IPMINLEVREVREILWEKIKEPTSQRKIILVIVSIALLLDNMLYMVIVPIIPDYLRYIGAW-------GEAGMDHVVTLPPIKEGNRTIIPTQI--------IPASHEGQDSATGILFASKAIVQLMINPFSGALIDRIGYDIPMMIGLIIMFLSTSIFACGRSYGMLFFARSLQGVGSAFADTSGLAMIADRFTEEAERSKALGIALAFISFGCLVAPPFGGALYQFAGKEVPFLILALISLMDGFMLLLVMKPLKTQMKEANEPKPNGTPIWKLLMDPYIAVCAGALMMSNVALAFLEPTISLWMEDNLTKENWKIGMIWLPAFFPHVLGVIITVKMARKYPQQQWLMAAGGLALEGLCCFIIPFATSYKMLMIPICGICFGIALIDTALLPTLGYLVDVRYVSVYGSIYAIADISYSFAYAVGPIIAGEVVEMIGFTALNLFIAFSNLLYAPVLMYLRHIYDFKPFE-----NEANILMSDPPDKEYQTYSMQDQKPV-NGEFKNHLEYSNMSGQIE 515

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BLAST of vesamicol binding vs. nr
Match: gi|930657230|gb|KPI99551.1| (Vesicular acetylcholine transporter [Papilio xuthus])

HSP 1 Score: 623.624 bits (1607), Expect = 0.000e+0
Identity = 306/528 (57.95%), Postives = 394/528 (74.62%), Query Frame = 0

Query:    6 LPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTNGYETKAYETTDMD------EEMNYD 527
            +P + +++ E R+  W+ ++EP +QR+++LV VSIALLLDNMLYMVIVPIIPDYLR IG W         A    +V+      GN T+I T +        I   ++G+D   G+LFASKA VQ++INP SG +ID+IGYD+PMM GLI+MF ST +FACG +Y +LFFARSLQGVGSAFADT G++MIADR+TEEAERSKA+GIALAFISFGCLVAPPFGG LY +AGK +PF+ILA + L+DGF+LLL+M+PLK +  E     PN TP+W+LL+D +IA C+GAL+++NV+LAFLEPTIS WM D +  + W+ G+IWLPAFFPHV+GVI+TV+MARKYP+  + +A  GL LEGL CF+IPF T+Y +LMIPIC ICFGIALIDTALLP LGY+VD +YVSVYGS+YAIADISYS AYAVGP+IAG +V  +GFTALN+F+A   L Y+P L YL+ ++D+ PFE     +   + + DPP KEYQT ++Q+ K + NG      E ++M       +E N D
Sbjct:   17 IPMINLEVREVREILWEKIKEPTSQRKIILVIVSIALLLDNMLYMVIVPIIPDYLRYIGAW-------GEAGMDHVVTLPPIKEGNRTIIPTQI--------IPASHEGQDSATGILFASKAIVQLMINPFSGALIDRIGYDIPMMIGLIIMFLSTSIFACGRSYGMLFFARSLQGVGSAFADTSGLAMIADRFTEEAERSKALGIALAFISFGCLVAPPFGGALYQFAGKEVPFLILALISLMDGFMLLLVMKPLKTQMKEANEPKPNGTPIWKLLMDPYIAVCAGALMMSNVALAFLEPTISLWMEDNLTKENWKIGMIWLPAFFPHVLGVIITVKMARKYPQQQWLMAAGGLALEGLCCFIIPFATSYKMLMIPICGICFGIALIDTALLPTLGYLVDVRYVSVYGSIYAIADISYSFAYAVGPIIAGEVVEMIGFTALNLFIAFSNLLYAPVLMYLRHIYDFKPFE-----NEANILMSDPPDKEYQTYSMQDQKPV-NGDFKNHLEYSNMSGQTEPVQESNVD 523

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BLAST of vesamicol binding vs. nr
Match: gi|1061483542|gb|ODN00757.1| (Vesicular acetylcholine transporter [Orchesella cincta])

HSP 1 Score: 620.928 bits (1600), Expect = 0.000e+0
Identity = 303/498 (60.84%), Postives = 382/498 (76.71%), Query Frame = 0

Query:    5 DLPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQE 502
             +P + ++L+E +      L++ KAQR++VLV VSIALLLDNMLYMVIVPIIPDYLR+IG W+T          +     + N TGNYT        +I  G  VI Y+GED  IG+LFASKA +Q+LINP SG +ID+IGYD PMM GL +MFFST +FA GS+YS+LFFARSLQGVGSAFADT G++MIADRYTEE ER+KA+GIALAFISFGCLVAPPFGG+LY  AGK +PF+IL+FVCL DG +L L+M+P+K +Q E+    P+ TP+W LL+D +IACC+GAL++ANVSLAFLEPTIS WM DTM   +WQQG+IWLPAFFPHV+GV+LTV++++ YP++ Y LA  GL LEGL C +IPF  +Y+LLMIPI  ICFGIALIDTALLP LGY+VD +YVSVYGS+YAIADISYS AYA GP+IAG ++ ++GFTALNI +A+  LAY P +++L+ ++D    +E        V + DPP K+YQT A+Q 
Sbjct:    3 QVPILNLELAEVKDFILAKLEDEKAQRKMVLVIVSIALLLDNMLYMVIVPIIPDYLRRIGSWDTHIEGGTRVTEAPRTITFTNQTGNYTYREAPPPPKIING--VIVYEGEDSAIGMLFASKALIQLLINPFSGAIIDRIGYDKPMMIGLTIMFFSTAVFAVGSSYSLLFFARSLQGVGSAFADTAGLAMIADRYTEERERTKALGIALAFISFGCLVAPPFGGVLYELAGKEVPFLILSFVCLADGIMLWLVMKPIKKQQKEQNVQKPDGTPIWVLLMDPYIACCAGALVMANVSLAFLEPTISVWMMDTMHVSQWQQGMIWLPAFFPHVLGVVLTVKLSKDYPQYQYLLAAGGLALEGLCCLMIPFAKSYWLLMIPISGICFGIALIDTALLPTLGYLVDLRYVSVYGSIYAIADISYSFAYAFGPIIAGRVMENIGFTALNIGIAVSNLAYVPIIFFLRHVYDKQFQDE------ANVIMKDPPDKQYQTYAMQN 492

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BLAST of vesamicol binding vs. nr
Match: gi|1229726672|ref|XP_022126121.1| (vesicular acetylcholine transporter [Pieris rapae])

HSP 1 Score: 619.772 bits (1597), Expect = 0.000e+0
Identity = 302/528 (57.20%), Postives = 393/528 (74.43%), Query Frame = 0

Query:    6 LPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESK----QMTNGYETKAYETTDMDEEMNYDEA 529
            +P + +++ E R+  WD ++EP +QR+++LV VSIALLLDNMLYMVIVPIIPDYLR IG W         A    +V+      GN T+I T +        I   ++G+D   G+LFASKA VQ++INP SG +ID+IGYD+PMM GLI+MF ST +FACG +Y +LFFARSLQGVGSAFADT G++MIADR+TEE+ERSKA+GIALAFISFGCLVAPPFGG LY +AGK +PF+ILA + L+DGF+LLL+M+PLK +  E     P+ TP+W+LL+D +IA C+GAL+++NV+LAFLEPTIS WM D +    W+ G+IWLPAFFPHV+GVI+TV+MA++YP+  + +A  GL LEGL CF+IPF T+Y +LMIPIC ICFGIALIDTALLP LGY+VD +YVSVYGS+YAIADISYS AYAVGP+IAG +V ++GFTALN+F+A   L Y+P L YL+ ++D+ PFE     +   + + DPP KEYQT ++Q+ +    +  N  E    +  D  EE N D +
Sbjct:   18 IPVVNLEVREVREVLWDKIKEPTSQRKIILVIVSIALLLDNMLYMVIVPIIPDYLRYIGAW-------GEAGFDHVVTLAPIQEGNRTIIPTKI--------IPASHEGQDSATGLLFASKAIVQLMINPFSGALIDRIGYDIPMMIGLIIMFLSTSIFACGRSYGMLFFARSLQGVGSAFADTSGLAMIADRFTEESERSKALGIALAFISFGCLVAPPFGGALYQFAGKEVPFLILALISLLDGFMLLLVMKPLKTQIKEAQQPKPDGTPIWKLLMDPYIAVCAGALMMSNVALAFLEPTISFWMEDHLTKDNWKIGMIWLPAFFPHVLGVIITVKMAKQYPQHQWLMAAGGLALEGLCCFIIPFATSYKMLMIPICGICFGIALIDTALLPTLGYLVDVRYVSVYGSIYAIADISYSFAYAVGPIIAGEVVEAIGFTALNLFIAFSNLLYAPVLMYLRHIYDFKPFE-----NEANILMADPPDKEYQTYSMQDQRPVNGEYKNHLEYSNVQGQDNVEESNVDSS 525

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BLAST of vesamicol binding vs. nr
Match: gi|241711519|ref|XP_002413424.1| (vesamicol binding protein, putative [Ixodes scapularis] >gi|215507238|gb|EEC16732.1| vesamicol binding protein, putative [Ixodes scapularis])

HSP 1 Score: 619.772 bits (1597), Expect = 0.000e+0
Identity = 310/543 (57.09%), Postives = 393/543 (72.38%), Query Frame = 0

Query:    6 LPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTNGYETKAYETTDMDEEMNYDEAPAPTNP-FKAQQASNPFRK 547
            LP +    S+  +R  D ++EPK+QRRL+L  V +ALLLDNMLYMVIVPIIP YLR IG W T T   N      + ++  NAT N   + TN T    +GG ++ Y+GE+  +GVLFASKA VQ+ INP SG +ID+IGYD+PMM GL +MF ST +FACG++Y VLFFARSLQGVGSAFADT G++MIADR+TEE+ERSKA+GIALAFISFGCLVAPPFGG+LY +AGK +PFIIL+ V L+DGFLLL +M+P+K +    G   P  TP+WRL ID +IAC +GAL+++NVSLAFLEPTIS WM D M   +WQ GLIWLPAFFPHV GV LTVR+AR+YP++ +A+A  GL LEGLS F++PF  +Y++L++P+  ICFGIA +DT+LLP LGY+VD +YVSVYGS+YAIADISYS AYA+GP+IAG IV S+GFTALNIF+A+  L Y P L  L+ ++DY PFE     S   + + DPP KEYQT  LQ+   M         +T+ M+             P  +AQQ   P ++
Sbjct:    4 LPIINQDTSQLWRRLNDKIEEPKSQRRLILFIVCVALLLDNMLYMVIVPIIPQYLRSIGSWYTHTEGGN------MSAFVVNATVNG--VATNRTEWRRMGGRIV-YEGEEAAVGVLFASKAIVQLFINPFSGALIDRIGYDIPMMVGLTIMFLSTAIFACGTSYGVLFFARSLQGVGSAFADTAGLAMIADRFTEESERSKALGIALAFISFGCLVAPPFGGLLYEFAGKEVPFIILSLVSLLDGFLLLFVMQPVKQQMRAMGMERPKGTPIWRLFIDPYIACAAGALMMSNVSLAFLEPTISIWMKDNMHVDDWQIGLIWLPAFFPHVAGVYLTVRLARQYPQYQWAMACFGLALEGLSSFIVPFARSYWVLILPLSGICFGIAQVDTSLLPTLGYLVDVRYVSVYGSIYAIADISYSLAYAIGPIIAGGIVESIGFTALNIFIALSNLLYCPLLMSLRRIYDYKPFE-----SEANILMQDPPAKEYQTYQLQDGTAMPLPVNNHVQQTSFMEGTTPVQTQQGVVKPNNQAQQQRPPHKR 532

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BLAST of vesamicol binding vs. nr
Match: gi|1279711257|ref|XP_022900019.1| (vesicular acetylcholine transporter [Onthophagus taurus])

HSP 1 Score: 619.002 bits (1595), Expect = 0.000e+0
Identity = 307/524 (58.59%), Postives = 387/524 (73.85%), Query Frame = 0

Query:    6 LPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHV-HPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTNGYETKAYETTDMDEEMNYDE 528
            +P + M+LSE +   W+ LQEP++QR+L+LV VSIALLLDNMLYMVIVPIIPDYLR IG +    I  N                  TV      +         ++ G+D   G+LFASKA VQ+++NP SG +ID+IGYD+PMM GL +MF ST +FACG +YS+LFFARSLQGVGSAFADT G++MIADR+TEE ERSKA+GIALAFISFGCLVAPPFGG LY +AGK +PF+ILAFV LIDGF+LLL+M+P+K  Q++E  V  P + P+WRL +D +IA CSGAL+++NV+LAFLEPTIS WM D +  + W+ G+IWLPAFFPHV GVILTV+MA+KYP + + +A  GL LEGL CF+IPF ++Y +LMIPIC ICFGIALIDTALLP LGY+VD +YVS+YGS+YAIADISYS AYA+GP+IAG +V ++GFTALN  +AI  L Y+P L YLKD++D+ PFE     +   + +GDPP KEYQT +LQ+  Q  N  E K +      +E N D 
Sbjct:    7 IPIINMELSELKDVVWNKLQEPRSQRKLILVIVSIALLLDNMLYMVIVPIIPDYLRYIGAYADDPIDEN-----------------VTVAPGEPFKH--------KHHGQDSATGILFASKAIVQLMVNPFSGALIDRIGYDVPMMIGLTIMFLSTAVFACGRSYSLLFFARSLQGVGSAFADTSGLAMIADRFTEENERSKALGIALAFISFGCLVAPPFGGALYQFAGKEVPFLILAFVSLIDGFMLLLVMKPIKT-QIKEAQVSKPPSVPIWRLFMDPYIAVCSGALMMSNVALAFLEPTISLWMEDNLTTENWKVGIIWLPAFFPHVFGVILTVKMAKKYPHYQWLMAACGLALEGLCCFIIPFASSYKVLMIPICGICFGIALIDTALLPTLGYLVDVRYVSIYGSIYAIADISYSVAYAIGPIIAGGVVEAIGFTALNFGIAISNLLYAPVLRYLKDIYDFKPFE-----NEANILMGDPPNKEYQTYSLQD-HQKVNTEEFKNHLEYSQQQETNLDR 498

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BLAST of vesamicol binding vs. nr
Match: gi|1285016576|ref|XP_023020437.1| (vesicular acetylcholine transporter [Leptinotarsa decemlineata])

HSP 1 Score: 617.461 bits (1591), Expect = 0.000e+0
Identity = 305/576 (52.95%), Postives = 400/576 (69.44%), Query Frame = 0

Query:    5 DLPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESK--------------QMTN---GYETKAYETT---------DMDEEMNYDEAPAPTNPFKAQ--------QASNPFR 546
            ++P + M+  E ++  W  LQEP+ QR+L+LV VSIALLLDNMLYMVIVPIIPDYLR IG ++   +  N +                  +     R+        ++ G+D   G+LFASKA VQ+++NP SG +ID+IGYD+PMM GL +MF ST +FACG +Y +LFFARSLQGVGSAFADT G++MIADR+TEE ERSKA+GIALAFISFGCLVAPPFGG LY +AGK +PF+ILAFV L+DGF+LLL+M+P+K +  E   + P + P+WRL +D +IA C+GAL+++NV+LAFLEPTIS W+ D +  + W+ G+IWLPAFFPHV GVI+TV+MA+KYP++ + +A  GL LEGL CF+IPF  +Y  LMIPIC ICFGIALIDTALLP LGY+VD +YVSVYGS+YAIADISYS AYAVGP+IAG +V ++GFTALN+ +A+  L Y+P L YL+ ++D+ PFE     +   + + DPP KEYQT ++QE K              Q TN   GY+++ Y+ T            ++  Y E P    P + Q        +A+NPFR
Sbjct:    3 NIPILNMEFRELKEAVWTKLQEPRTQRKLILVIVSIALLLDNMLYMVIVPIIPDYLRYIGAYQDYALSGNES------------------LPPGTPRKH-------DHHGQDQATGILFASKAIVQLMVNPFSGALIDRIGYDIPMMIGLTIMFLSTAVFACGRSYGLLFFARSLQGVGSAFADTAGLAMIADRFTEETERSKALGIALAFISFGCLVAPPFGGALYQFAGKEMPFLILAFVSLMDGFMLLLVMKPIKDQIKESQMMKPPSVPIWRLFMDKYIAVCAGALMMSNVALAFLEPTISLWVEDNLTTENWKVGIIWLPAFFPHVFGVIITVKMAKKYPQYQWLMAAAGLALEGLCCFIIPFANSYVFLMIPICGICFGIALIDTALLPTLGYLVDVRYVSVYGSIYAIADISYSVAYAVGPIIAGSVVEAIGFTALNVGIALMNLFYAPVLMYLRHIYDFKPFE-----NEANILMSDPPNKEYQTYSMQEQKPVGGEIQNHLEYKQQETNFDSGYQSQDYQHTGYQQNYQQRSYQQDQPYQETPFQQPPARPQGRVLPQQPEAANPFR 548

[Close]

BLAST of vesamicol binding vs. nr
Match: gi|829580885|gb|AKL78874.1| (vesicular acetylcholine transporter [Chilo suppressalis])

HSP 1 Score: 617.076 bits (1590), Expect = 0.000e+0
Identity = 301/529 (56.90%), Postives = 396/529 (74.86%), Query Frame = 0

Query:    6 LPFMGMKLSEARQRCWDYLQEPKAQRRLVLVTVSIALLLDNMLYMVIVPIIPDYLRKIGVWETRTIHNNNAQTSTLVSYYNNATGNYTVITTNMTRRINVGGIVIEYDGEDMGIGVLFASKAAVQILINPMSGMVIDKIGYDLPMMFGLIVMFFSTLLFACGSTYSVLFFARSLQGVGSAFADTGGMSMIADRYTEEAERSKAMGIALAFISFGCLVAPPFGGILYNWAGKALPFIILAFVCLIDGFLLLLIMRPLKAKQVEEGHVHPNATPLWRLLIDSHIACCSGALIVANVSLAFLEPTISKWMGDTMDAKEWQQGLIWLPAFFPHVMGVILTVRMARKYPEWVYALAGIGLVLEGLSCFVIPFCTNYFLLMIPICFICFGIALIDTALLPMLGYIVDKKYVSVYGSVYAIADISYSAAYAVGPVIAGHIVNSMGFTALNIFVAIFTLAYSPCLYYLKDMHDYNPFEEGLDGSGGGVTIGDPPTKEYQTIALQESKQMTNGYETKAYETTDMDEEMNYDEAPAPTN 534
            +P + +++ E R+  W+ ++EP++QR+++LV VSIALLLDNMLYMVIVPIIPDYLR IG W      +       +V+      GN T+I T +        I   ++G++   GVLFASKA VQ++INP SG +ID+IGYD+PMM GL++MF ST +FACG +YS+LFFARSLQGVGSAFADT G++MIADR+TEEAERSKA+GIALAFISFGCLVAPPFGG LY +AGK +PF+ILA + LIDGF+LLL+M+PLK +  E        TP+W+LL+D +IA C+GAL+++NV+LAFLEPTIS WM D +    W+ G+IWLPAFFPHV+GVI+TV+MA+KYP+  + +A  GL LEGL CF+IPF  +Y +LMIPIC ICFGIALIDTALLP LGY+VD +YVSVYGS+YAIADISYS AYAVGP+IAG +V ++GFTALN+ +A   L Y+P L YL+ ++D+ PFE     +   + +GDPP KEYQT +LQ+ + + NG      E +++  ++  +E+   TN
Sbjct:   17 IPVVNLEVREVREVLWEKIKEPRSQRKIILVIVSIALLLDNMLYMVIVPIIPDYLRYIGAWGEEAYDH-------VVTLPPIREGNRTIIPTKI--------IPATHEGQESATGVLFASKAIVQLMINPFSGALIDRIGYDIPMMIGLVIMFLSTSVFACGRSYSMLFFARSLQGVGSAFADTSGLAMIADRFTEEAERSKALGIALAFISFGCLVAPPFGGALYQFAGKEVPFLILALISLIDGFMLLLVMKPLKTQIYEYNQPKTAGTPIWKLLMDPYIAVCAGALMMSNVALAFLEPTISLWMEDNLTKDNWKIGMIWLPAFFPHVLGVIITVKMAKKYPQHQWLMAAGGLALEGLCCFIIPFANSYKMLMIPICGICFGIALIDTALLPTLGYLVDVRYVSVYGSIYAIADISYSFAYAVGPIIAGEVVEAIGFTALNLLIAFSNLLYAPVLMYLRHIYDFKPFE-----NEANILMGDPPDKEYQTYSLQDQRPV-NGEYKNHLEYSNVSGQVGTEESNVDTN 524

The following BLAST results are available for this feature:

BLAST of vesamicol binding vs. L. salmonis genes
BLAST of vesamicol binding vs. SwissProt
BLAST of vesamicol binding vs. nr

BLAST of vesamicol binding vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides)
Total hits: 4


Match Name	E-value	Identity	Description
EMLSAG00000012699	0.000e+0	68.62	supercontig:LSalAtl2s:LSalAtl2s964:5967:7469:-1 ge...	[more]
EMLSAG00000006135	5.530e-98	36.05	supercontig:LSalAtl2s:LSalAtl2s334:392742:405619:-...	[more]
EMLSAG00000009550	3.981e-50	32.35	supercontig:LSalAtl2s:LSalAtl2s615:180941:184069:1...	[more]
EMLSAG00000007792	3.879e-7	24.09	supercontig:LSalAtl2s:LSalAtl2s457:18904:31426:-1 ...	[more]

BLAST of vesamicol binding vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt)
Total hits: 23


Match Name	E-value	Identity	Description
gi\|34924873\|sp\|O17444.2\|VACHT_DROME	0.000e+0	57.31	RecName: Full=Vesicular acetylcholine transporter;...	[more]
gi\|464998\|sp\|P34711.1\|UNC17_CAEEL	5.162e-174	49.82	RecName: Full=Vesicular acetylcholine transporter ...	[more]
gi\|34924880\|sp\|P81721.2\|VACHT_TORCA	5.677e-170	50.10	RecName: Full=Vesicular acetylcholine transporter;...	[more]
gi\|34924981\|sp\|Q91498.2\|VACHT_TORMA	2.108e-169	49.01	RecName: Full=Vesicular acetylcholine transporter;...	[more]
gi\|34924984\|sp\|Q91514.1\|VACHT_TORTO	1.101e-168	49.51	RecName: Full=Vesicular acetylcholine transporter;...	[more]
gi\|123884384\|sp\|Q08C75.1\|VACHA_DANRE	2.990e-166	46.28	RecName: Full=Probable vesicular acetylcholine tra...	[more]
gi\|34924877\|sp\|P59845.1\|VACHB_DANRE	4.523e-157	49.70	RecName: Full=Probable vesicular acetylcholine tra...	[more]
gi\|34924861\|sp\|Q62666.2\|VACHT_RAT	2.206e-155	53.38	RecName: Full=Vesicular acetylcholine transporter;...	[more]
gi\|34924876\|sp\|O35304.1\|VACHT_MOUSE	2.432e-155	53.15	RecName: Full=Vesicular acetylcholine transporter;...	[more]
gi\|313104043\|sp\|Q16572.2\|VACHT_HUMAN	8.665e-155	53.91	RecName: Full=Vesicular acetylcholine transporter;...	[more]

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BLAST of vesamicol binding vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR)
Total hits: 25


Match Name	E-value	Identity	Description
gi\|1325327285\|ref\|XP_023345498.1\|	0.000e+0	69.82	vesicular acetylcholine transporter-like isoform X...	[more]
gi\|1022766571\|gb\|KZS11615.1\|	0.000e+0	60.00	Vesicular acetylcholine transporter [Daphnia magna...	[more]
gi\|943934311\|ref\|XP_014357236.1\|	0.000e+0	58.27	PREDICTED: vesicular acetylcholine transporter [Pa...	[more]
gi\|930657230\|gb\|KPI99551.1\|	0.000e+0	57.95	Vesicular acetylcholine transporter [Papilio xuthu...	[more]
gi\|1061483542\|gb\|ODN00757.1\|	0.000e+0	60.84	Vesicular acetylcholine transporter [Orchesella ci...	[more]
gi\|1229726672\|ref\|XP_022126121.1\|	0.000e+0	57.20	vesicular acetylcholine transporter [Pieris rapae]	[more]
gi\|241711519\|ref\|XP_002413424.1\|	0.000e+0	57.09	vesamicol binding protein, putative [Ixodes scapul...	[more]
gi\|1279711257\|ref\|XP_022900019.1\|	0.000e+0	58.59	vesicular acetylcholine transporter [Onthophagus t...	[more]
gi\|1285016576\|ref\|XP_023020437.1\|	0.000e+0	52.95	vesicular acetylcholine transporter [Leptinotarsa ...	[more]
gi\|829580885\|gb\|AKL78874.1\|	0.000e+0	56.90	vesicular acetylcholine transporter [Chilo suppres...	[more]

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Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
scaffold22_size673200	supercontig	scaffold22_size673200:565388..567031 -

Analyses

This gene is derived from or has results from the following analyses


Analysis Name	Date Performed
maker	2018-02-12 .496401
T. kinsejongensis vs L. Salmonis peptides	2018-04-19
T. kingejongensis peptided Blastp vs. SwissProt	2018-04-19
T. kingsejongensis proteins Blastp vs. NR	2018-05-15

Properties


Property Name	Value
Note	P:drug transport

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Species	Type
snap_masked-scaffold22_size673200-processed-gene-5.22-mRNA-1	snap_masked-scaffold22_size673200-processed-gene-5.22-mRNA-1	Tigriopus kingsejongensis	mRNA

Sequences

The following sequences are available for this feature:

gene from alignment at scaffold22_size673200:565388..567031-

gene from alignment at scaffold22_size673200:565388..567031-

Legend: mRNA

Hold the cursor over a type above to highlight its positions in the sequence below.

>snap_masked-scaffold22_size673200-processed-gene-5.22 ID=snap_masked-scaffold22_size673200-processed-gene-5.22|Name=vesamicol binding|organism=Tigriopus kingsejongensis|type=gene|length=1644bp|location=Sequence derived from alignment at scaffold22_size673200:565388..567031- (Tigriopus kingsejongensis)
ATGCCCGACTTTGACTTGCCCTTCATGGGCATGAAGCTCTCAGAGGCCAG
GCAGAGATGCTGGGATTACCTCCAGGAGCCCAAAGCCCAACGGAGGCTCG
TGCTCGTCACCGTCTCCATTGCCCTGCTTCTGGACAACATGCTCTACATG
GTGATTGTGCCCATCATCCCCGACTACCTCCGAAAGATCGGAGTCTGGGA
GACGCGTACTATCCACAACAACAACGCTCAGACCAGCACCTTGGTCTCCT
ACTACAACAACGCCACGGGGAACTACACCGTCATCACCACCAACATGACG
CGGAGGATCAACGTGGGCGGCATCGTCATCGAGTACGACGGCGAGGACAT
GGGGATTGGCGTGCTCTTCGCCTCCAAGGCCGCGGTGCAGATCCTCATCA
ACCCCATGTCCGGAATGGTGATCGACAAGATTGGCTACGACCTCCCCATG
ATGTTTGGCCTCATCGTGATGTTCTTCTCCACCCTGCTCTTCGCCTGTGG
TTCCACCTACTCGGTCCTCTTCTTCGCTCGGAGTCTCCAAGGCGTGGGCT
CAGCCTTTGCTGATACGGGTGGGATGTCCATGATTGCTGACCGCTACACA
GAGGAGGCCGAACGTTCCAAGGCCATGGGCATAGCGCTGGCCTTCATCTC
GTTCGGGTGCCTGGTGGCGCCACCCTTCGGAGGCATCCTGTACAATTGGG
CGGGCAAGGCCCTGCCCTTCATCATCCTGGCCTTCGTGTGTCTCATCGAC
GGATTCCTCCTCCTTCTCATCATGCGGCCACTGAAGGCCAAACAAGTGGA
GGAGGGCCATGTCCACCCCAACGCCACGCCCCTCTGGCGTCTGCTCATCG
ACTCGCACATTGCGTGCTGCAGCGGGGCCCTCATTGTGGCCAACGTGTCC
TTGGCCTTCTTGGAGCCCACCATCTCCAAGTGGATGGGCGACACCATGGA
TGCGAAGGAGTGGCAGCAAGGTCTCATCTGGCTCCCAGCCTTCTTCCCCC
ACGTCATGGGGGTGATTCTCACCGTCAGAATGGCCAGGAAATACCCAGAG
TGGGTGTACGCGTTGGCCGGCATTGGTCTCGTCCTCGAGGGCTTGTCGTG
CTTCGTGATTCCTTTCTGCACCAATTACTTCCTCCTCATGATCCCCATCT
GCTTTATTTGTTTTGGCATCGCCCTAATCGACACTGCTCTTCTCCCCATG
CTGGGCTACATCGTGGACAAGAAGTACGTGTCCGTCTACGGCTCGGTCTA
CGCCATCGCAGACATCTCCTACTCGGCGGCTTACGCCGTTGGTCCGGTCA
TAGCGGGACACATCGTGAACTCCATGGGCTTCACTGCCCTCAACATCTTT
GTGGCTATTTTTACCCTAGCCTACTCCCCATGCCTCTACTACCTGAAGGA
CATGCACGACTACAACCCCTTCGAGGAGGGACTGGATGGGAGTGGCGGAG
GTGTGACCATCGGCGATCCACCCACCAAGGAGTACCAGACGATCGCACTT
CAGGAGTCCAAACAGATGACCAACGGCTACGAGACCAAGGCGTACGAGAC
CACCGACATGGACGAGGAGATGAACTACGACGAGGCCCCGGCCCCCACCA
ACCCCTTCAAGGCCCAGCAGGCGAGCAATCCCTTTAGGAAATGA

Synonyms

The feature 'vesamicol binding' has the following synonyms


Synonym
Tk12703

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