BLAST of aconitate hydratase 1 vs. L. salmonis genes
Match: EMLSAG00000000118 (supercontig:LSalAtl2s:LSalAtl2s101:496791:499884:-1 gene:EMLSAG00000000118 transcript:EMLSAT00000000118 description:"augustus_masked-LSalAtl2s101-processed-gene-5.8")

HSP 1 Score: 830.861 bits (2145), Expect = 0.000e+0
Identity = 424/869 (48.79%), Postives = 575/869 (66.17%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDAD---YAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAET-----FRRPMGKE---VTVVGED---YTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYT-KGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNL 875
            + KLP +++++LE+ +R  D  F V   D++    W    GKN  EI +RP RV++QDFTGVPA+VD AAMRD +  LGGD  +INP  P DLVIDHS+ +D     +A   N D E++RN ER+TFLKWG  +F N  ++PPG+GI HQVNLEYL++  +TDTD     + +PD++VGTDSHTTM+NGL V+GWGVGGIEAEA MLGQP+SM++PEVVG+ ++G        TD+VL + + LR  GVVG+FVEFYG G+  L +ADRATI+NM PEYGAT G FP+D  ++ YLR T R+  ++  ++ Y       RD +     P ++  + LD+GT+V + SGPKRPQD VA+T+ K  F + ++       F  P  K+   V ++  D   Y +  G V+IA+ITSCTNTSNP VM+GAGL+A+KA   GL   P++KTSL+PGS VV+ YL+ +G+   LD +GF++VGYG  TCIGNSGP++  +     +GDLV   VLSGNRNFEGRI+P  RANYLASPPLV+AYA+AG +DID   EALG D N   ++L+DIWPT  EI  + +K +    F   Y+ V  G+E+W +++ P    Y W   STYI++PP+F  MS++  +   +  A  L  LG   T DHISPAG    + PA +YL  + +  REFNSYGSRRGN  +M RGTFANIR+ N+ L G  G  T     G    +FDAAM ++ E   L++ AG+ YG GSSRDWAAKG  LLGV+AVIAE++ERIHRSNLVGMG++P +++ G+S +SLG +G E  ++     + P Q +   +   DGS K      R DT +E+ Y  +GG+L+Y++R +
Sbjct:   31 YHKLPYSIRILLESAVRNCDN-FQVYEKDVETILNWEGNRGKN-AEIPFRPGRVILQDFTGVPAIVDFAAMRDAVKKLGGDPSKINPTCPTDLVIDHSIQVDFARESKALMKNEDLEFQRNKERFTFLKWGASSFQNMLIIPPGSGIVHQVNLEYLARVCFTDTD-----LVFPDSVVGTDSHTTMINGLGVVGWGVGGIEAEAVMLGQPISMVLPEVVGYRISGSPQPLVNSTDIVLTITKHLRQVGVVGRFVEFYGPGIKHLSIADRATISNMCPEYGATIGXFPVDLVSIEYLRLTNRESSKVKCIQEYLHLTNLLRDYEDKSTDPTFSQIVVLDLGTVVTSCSGPKRPQDRVAVTDMKNDFLQCLSNKIGFKGFAIPDNKKNITVPIIAPDNKEYILRHGSVLIAAITSCTNTSNPSVMLGAGLLAKKAVDKGLTVPPYIKTSLSPGSGVVTYYLKNSGVIPYLDALGFSIVGYGXMTCIGNSGPLEDYVVXGXNKGDLVCAGVLSGNRNFEGRINPHTRANYLASPPLVIAYAIAGRVDIDFEVEALGFDANNEPIYLRDIWPTRDEIQIVEKKYIVPSMFEDVYSKVTTGNERWNSLEAPKGILYPWDIESTYIKSPPFFDNMSEELPEPKKIKNALALLNLGXSFTXDHISPAGXIARNXPAARYLASKNISPREFNSYGSRRGNDAIMARGTFANIRLVNKFLGGKPGPKTIHLISGDIMDVFDAAMEYKKENQHLIVLAGKDYGCGSSRDWAAKGPFLLGVRAVIAESYERIHRSNLVGMGIVPLQYSEGESSESLGFSGKEKFSVELPTNLIPQQVLDIKVECLDGSIKSFKATVRFDTELELTYYRHGGILNYMIRKV 892          

BLAST of aconitate hydratase 1 vs. L. salmonis genes
Match: EMLSAG00000007801 (supercontig:LSalAtl2s:LSalAtl2s457:64181:67196:1 gene:EMLSAG00000007801 transcript:EMLSAT00000007801 description:"maker-LSalAtl2s457-snap-gene-0.30")

HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0
Identity = 375/898 (41.76%), Postives = 535/898 (59.58%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMID------EFGNP-------------------------RAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAP--IYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFT-----REMAETFRRPMGK-----EVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGP-IQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALG-QDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDS-ETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYT-KGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVL 872
            + +LP +L+V+LE  +R                SE   +  K+  E   R  RVL+QDFTGVPA++DLAA+R+ +   GG    ++ L P DLV+DH V +D      +  +P                          A + N   E+ERN ER+ FLKW   AF N  V+PPGTG+ HQVNLEYL++ V   T Q GV   +PD++VGTD+HTTM+NGL VLGWGVG ++A+A M G PV++  P VVG  + G + + +T TDLVL + + LR  GV G FVEF+G  L  L +ADRATI+NM PEYGA  GFFPID  T++YL  +GRD+  I  VE Y K    +R +D      + + + +D+  I+P +SGPKR +D V++      F      +E  + +    G+      V + GE +T+  G V++A+I+SCTNTSNP VM+GAGL+A+KA   GL+    VKTSLAPGS VV+ YL+ +G+   L  +GF +VGYGC+TC  NSGP +   +  AI QG+LV   +LSGNRNFEGR+ PD+RANYLASP +V+AYA+AG +DI+L  E LG     G  +FLK+IWP+  EI  +  K V    + + Y+ V  G++ W ++  P+S + + W + STYI+ P Y + +  +     N+   + L  LGD +T+DHISPAGS   S+PA +YL    +  RE+NSYG+RRGN+ VM+RGTF+N+R++N +L+ V G YT   P      IF+A+  ++ +G  L    G+ +G G+SRDWA +G  LLGV+A++A +F+  +RSNLV  G++P + T   S     L G E ++I   D I P   +   +  A+ S K +    R+D   E +    GGV+  ++
Sbjct:   37 YEELPYSLRVLLECAVRKR--------------SETQGQVWKDVYERILR--RVLLQDFTGVPALIDLAAIRESVSKSGGCPDVVDSLCPADLVVDHCVQVDYNQIKQQLSSPPDPSPKTPVLGDDICPFHQKMSYWADALRSNQTSEFERNKERFIFLKWVNSAFKNITVIPPGTGVMHQVNLEYLARVV---TCQGGV--LFPDSVVGTDTHTTMINGLGVLGWGVGTLDAQAVMFGHPVTLSFPRVVGIRIKGVISDYSTSTDLVLMITKKLRQAGVQGAFVEFFGPSLKELSVADRATISNMCPEYGALVGFFPIDEITIQYLAQSGRDKSHIETVEKYMKTVKMFRSSDENENIRFASIIEIDLSDIIPCVSGPKRTKDKVSINEIAADFKSALHLKEGPKGYGLSQGQGHVAFSVNIDGEYFTVGHGTVLLAAISSCTNTSNPSVMLGAGLLAKKAVECGLSVPKVVKTSLAPGSGVVTSYLQESGVMPYLYMLGFEIVGYGCSTCSYNSGPNLPDSIVQAIKQGNLVCCGLLSGNRNFEGRLDPDIRANYLASPLMVIAYAIAGRIDINLDREPLGFNSHTGEAIFLKNIWPSRQEIQEIERKLVIPGIYRQVYSKVSVGNKHWDSLSVPNSVDKFPWSSDSTYIKCPEYIKEIGSNARNWKNM---RCLLKLGDNITSDHISPAGSITRSSPAAEYLSSLGLAPREYNSYGARRGNYHVMVRGTFSNMRLQNHLLNKV-GPYTLHLPSEVTTTIFEASERYKNDGVSLFAIGGKNFGRGTSRDWATRGPFLLGVRAILAVSFDPTYRSNLVKTGILPLQIT---SISYESLKGTECLSIDIRDNITPKCLIDVVVGKAERSFKAVV---RVDNDYETDVFHAGGVIAQIV 903          

BLAST of aconitate hydratase 1 vs. L. salmonis genes
Match: EMLSAG00000008083 (supercontig:LSalAtl2s:LSalAtl2s481:171581:178388:-1 gene:EMLSAG00000008083 transcript:EMLSAT00000008083 description:"maker-LSalAtl2s481-augustus-gene-1.18")

HSP 1 Score: 132.494 bits (332), Expect = 1.190e-31
Identity = 141/493 (28.60%), Postives = 216/493 (43.81%), Query Frame = 0

Query:   71 RPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTL-VGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIAS--ITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRAN-YLASPPLVVAYALAGTMDIDLATEAL 559
            RP RV MQD T   A++   +     VA+           P  +  DH +     G     + ++ R  + N E Y FL      +       PG+GI HQ+ LE                 A+P  L VGTDSHT    GL  L  GVGG +A   M   P  +  P+V+G +LTG++   T+  D++LKV  +L   G  G  VE++G G+D +      TI NM  E GAT   FP +     YL +T R  D   L + Y +        D    Y   + +D+ T+ P ++GP               FT ++A     P+G+    +GE        + +    I SCTN+S   +     ++ ++A A G+  K      + PGS+ +   +E  GL + + + G  ++   C  CIG            I +G+   T V S NRNF GR   +   + ++ SP + VA +LAG++D D  ++A+
Sbjct:  675 RPDRVAMQDATAQMAMLQFISSGLPKVAV-----------PSTIHCDHLIQAQIEG-----KKDLARAKDLNAEVYNFLSTSGAKYG-VGFWKPGSGIIHQIILE---------------NYAFPGLLMVGTDSHTPNGGGLGGLCIGVGGADAVDVMADIPWELKCPKVIGVKLTGKLSGWTSPKDVILKVAGILTVKGGTGAIVEYFGEGVDSISCTGMGTICNMGAEIGATTSVFPYNHRMKAYLDSTNR-SDIANLADKYRE---VLLSPDGGCKYDQVVEIDLNTLEPHVNGP---------------FTPDLAT----PIGE----LGEKAKKNGWPLEVKDGLIGSCTNSSYEDMARXTAII-KQALAKGMKSKS--NFYITPGSEQIRATIERDGLADTMREFGGVVLANACGPCIGQWD------RQNITKGE-KNTIVTSYNRNFTGRNDANPATHGFVTSPEMTVALSLAGSLDFDPRSDAI 1098          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|81600603|sp|Q5SMF6.1|ACNA_THET8 (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 1006.9 bits (2602), Expect = 0.000e+0
Identity = 514/879 (58.48%), Postives = 648/879 (73.72%), Query Frame = 0

Query:   15 EAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEW-ADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWR--DADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRR--------PMGKEVTVV--GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKG-PDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            E  G+ + S+LP +++V+LE++LR EDG + V+ +DI+A + W  D G  N   +  + ARV++QDFTGVPAVVDLAAMRD I A GGD ++INP+ P DLVIDHSV +D FG   AF  NV++EYERN ERY  LKW Q A  NFRVVPPGTGI HQVN+EYL++ V T   ++G+ +A+PD+LVGTDSHTTMVNGL VLGWGVGGIEAEA MLGQP  ML P VVGF+L G++ EG T TDLVL V EMLR HGVVGKFVEFYG G+  L   DRATIANMAPEYGAT GFFP+D ETL YLR TGR E+ + LVEAY K  G +R  +A+    Y+  L LD+  + P+++GPKRPQD V L   K +F   + +  +          + ++V V    E++ +  G VVIA+ITSCTNTSNP VM+GAGL+A+KA   GL+RKPWVKTSLAPGS+VV++YLE +GL   L+ +GF+LVGYGCTTCIGNSGP+ ++++ A+ +G+LV  +VLSGNRNFEGRI+P V+ANYLASP LVVAYALAG MDID  TE LG D NG  ++LKDIWP+  EI   + KT+  E F K+Y+ VF+GDE+WQA+  P  E Y W   STYIQNPP+F+ + +   K+ ++ GA+VL VLGD VTTDHISPAG+    +PAGQYLI + V   +FNSYGSRRGNHEVMMRGTFANIRIKN MLDG+EGGY K  P+G    +++ AM ++AEGTPL++ AG++YG GSSRDWAAKGT LLG++AV+AE+FERIHRSNLVGMGV+P EF  G++R++LGLTG E   I GL+ ++P + V       DGS        R+DT VE++Y +NGG+L  VL N+ K A
Sbjct:   27 ERKGVAEVSRLPFSIRVMLESLLRNEDG-YQVTREDIEALARWRPDPGEIN---VPLKLARVILQDFTGVPAVVDLAAMRDAIKAKGGDPKRINPVVPADLVIDHSVQVDAFGTAYAFFYNVEKEYERNRERYLLLKWAQNALENFRVVPPGTGIVHQVNIEYLTKVVMTG-KRDGLTLAFPDSLVGTDSHTTMVNGLGVLGWGVGGIEAEAVMLGQPYYMLAPRVVGFKLYGELPEGATATDLVLTVTEMLRKHGVVGKFVEFYGPGVAKLSTPDRATIANMAPEYGATMGFFPVDEETLNYLRQTGRPEELVELVEAYTKAVGLFRTPEAEEKVQYSEYLELDLSAVEPSLAGPKRPQDRVPLKEVKKSFLAHLTKPVKERGFGLSEDQLQRKVLVKRRDEEFELTHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVEAGLDRKPWVKTSLAPGSKVVTDYLEMSGLMPFLEALGFHLVGYGCTTCIGNSGPLPEDIAKAVEEGNLVVAAVLSGNRNFEGRINPHVKANYLASPMLVVAYALAGRMDIDFTTEPLGFDPNGKPIYLKDIWPSMEEIREAIRKTLDPELFKKEYSKVFEGDERWQALPAPTGELYQWDPESTYIQNPPFFEDLGER--KVEDIRGARVLLVLGDSVTTDHISPAGAIPVKSPAGQYLISKGVKPEDFNSYGSRRGNHEVMMRGTFANIRIKNLMLDGIEGGYAKKLPEGDVDFVYNVAMRYKAEGTPLLVIAGKEYGTGSSRDWAAKGTYLLGIRAVLAESFERIHRSNLVGMGVLPLEFLPGENRETLGLTGYEVYDILGLEDLKPRKLVDIVARREDGSEVRFQAIARLDTPVEVDYYKNGGILQTVLLNMLKEA 898          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|81624827|sp|Q9RTN7.1|ACNA_DEIRA (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein; Flags: Precursor)

HSP 1 Score: 1003.43 bits (2593), Expect = 0.000e+0
Identity = 508/870 (58.39%), Postives = 642/870 (73.79%), Query Frame = 0

Query:   21 DFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPR-EIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWR-DADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRR--------PMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKG-PDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRN-LSKA 878
            D S+LP ++KV+LE++LR E   + V  +D++  + W+     NP  EI ++PARV++QDFTGVPAVVDLAAMR  +V LGGD  +INPL PVDLVIDHSV +DEFG   A   N+  E+ERN ERY FL+WGQ+AF+NF VVPP +GI HQVNLEYL++ V +  + +G E+ YPD+LVGTDSHTTM+NGL ++GWGVGGIEAEA MLGQP+ ML+PEV+GF++TG M EG T TDL L+V +MLR  GVVGKFVEFYGAGL  + L DRATIANMAPEYGAT GFFP+D E LRYLR TGR ED I LVEAY K  G +R D    P++T T+ LD+ TIVP+++GPKRPQD V L++  + F   +    +          +  + T+ G D  +  G V +ASITSCTNTSNP V+I AGLVA+KA   GL  KPWVKTSLAPGS+VV+EYLE AGLQ+ LD+IGFN VGYGC TCIGNSGP+ + + +AI +GDLV  SVLSGNRNFEGR++P ++ANYLASPPLVVAYALAGT+  D+  +A+GQD NG DVFLKDIWPT+AEI   +++++  E F K Y  + K +  W A+   +   +DW   STYIQNPP+F  ++    +I ++ GA+ L  +GD VTTDHISPAGSFK  TPAG+YL ER +  ++FNSYGSRRGN  +M RGTFANIR+KN++  G EGG+T    +G   +IFDA+ A++  G PLV+ AG+ YG GSSRDWAAKGT LLGVKAVIAE+FERIHRSNLVGMGV+P ++  G++  SLG+ GDET        ++P Q+VT  +T  DG+ ++ITV CRIDT VEI+Y +NGG+L  VLR  LSK+
Sbjct:   36 DVSRLPVSIKVLLESVLR-EANDYDVRREDVETVAGWS---ATNPEVEIPFKPARVILQDFTGVPAVVDLAAMRSAMVKLGGDPSKINPLIPVDLVIDHSVQVDEFGTEFALANNMALEFERNRERYEFLRWGQQAFDNFGVVPPASGIVHQVNLEYLAKGVQSRAEDDG-EVVYPDSLVGTDSHTTMINGLGIVGWGVGGIEAEAVMLGQPIYMLMPEVIGFKITGAMPEGATATDLALRVTQMLREKGVVGKFVEFYGAGLSNMTLPDRATIANMAPEYGATMGFFPVDDEALRYLRRTGRLEDEIGLVEAYYKAQGMFRTDETPDPVFTDTIELDLATIVPSLAGPKRPQDRVNLSDMHSVFNEALTAPVKNRGFELGSDKLDAQGTIGGTDIKIGHGAVTLASITSCTNTSNPSVLIAAGLVAKKAVEKGLKTKPWVKTSLAPGSRVVTEYLETAGLQQYLDQIGFNTVGYGCMTCIGNSGPLPEPVVEAIQEGDLVVASVLSGNRNFEGRVNPHIKANYLASPPLVVAYALAGTVVNDIVNDAIGQDSNGQDVFLKDIWPTNAEIQEAMDRSINAEMFKKVYDGIEKSNADWNAIPVAEGALFDWKEDSTYIQNPPFFDTLAGGAHEIESIKGARALVKVGDSVTTDHISPAGSFKADTPAGRYLTERGIAPKDFNSYGSRRGNDRIMTRGTFANIRLKNQLAPGTEGGFTTNFLNGEVTSIFDASTAYKEAGVPLVVLAGKDYGMGSSRDWAAKGTFLLGVKAVIAESFERIHRSNLVGMGVLPLQYKNGETADSLGINGDETFEFVLPGDLKPRQDVTVKVTGKDGNTRDITVMCRIDTPVEIDYYKNGGILQTVLRGILSKS 900          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|81564851|sp|Q6NH63.1|ACNA_CORDI (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 998.423 bits (2580), Expect = 0.000e+0
Identity = 523/915 (57.16%), Postives = 639/915 (69.84%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPI--YTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFR----------RPMGKE--VTVVGED------------------------------------YTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKG--PDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQN------VTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKA 878
              KLP +LKV+ EN+LR EDG  +++ D I A + W D   +   EI + PARVLMQDFTGVP VVDLA MR+ +  LGGD  ++NPLNP ++VIDHSV+I+ FG+  A   NV+ EYERN ERY FL+WG KAF+NFRVVPPGTGI HQVN+E L++ V+   D NG  +AYPDT +GTDSHTTM NGL +LGWGVGGIEAEAAMLGQPVSMLIP VVGF+LTG++  G T TD+VL + EMLR HGVV KFVEFYG G+  +PLA+RATI NM+PE+G+TC  FPID ET++Y+  TGR E+++ALVEAYAK  G W + D AP   Y+  L LD+ T+VP+I+GPKRPQD + LT AK  F +++ +             + M  E  V   GED                                    YT++ G V IASITSCTNTSNP VMIGAGL+ARKAAA GL  KPWVKT  APGSQVV  Y + A L +DL+ +GF L G+GCTTCIGNSGP+ +E+S AI + DL AT+VLSGNRNFEGRISPDV+ NYL SP +V+AYA+AGTMD D  T+ LGQD +GNDVFLKDIWP++ EI   +   ++RE +   YADVFKGDE+WQ + TP+ +T+DW   STYI+  PYF GM+ +P  +S++ GA+VLA LGD VTTDHISPA S K  TPA QYL E  V   ++NS GSRRGNHEVMMRGTFANIR++N+++D + GGYT+    +G QA IFDA   ++A G PLV+ AG++YG GSSRDWAAKGT LLGVKAVI E+FERIHRSNL+GMGVIP +F  G+S  SLGL G ET  I G   IE L N      V  T T   G   E     RIDT  E +Y  NGG+L YVLRN+  A
Sbjct:   32 MEKLPYSLKVLGENLLRTEDGA-NITADHINAIANW-DPSAEPSIEIQFTPARVLMQDFTGVPCVVDLATMREAVKTLGGDPDKVNPLNPAEMVIDHSVIIEAFGSTLALAKNVEIEYERNEERYQFLRWGSKAFSNFRVVPPGTGIVHQVNIENLARVVF---DNNG--LAYPDTCIGTDSHTTMENGLGILGWGVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIPAGVTATDVVLTITEMLREHGVVQKFVEFYGNGVKSIPLANRATIGNMSPEFGSTCAIFPIDEETVKYMHLTGRSEEQVALVEAYAKAQGMWLEQD-APEAEYSEYLELDLSTVVPSIAGPKRPQDRILLTEAKEQFRKDLPDYCSAEPVDESLPAKRMDSEGAVQKEGEDVAGYNSSRAGHGESAAEGAAGRQSNPVVVSSPNGGEYTLDHGMVAIASITSCTNTSNPSVMIGAGLIARKAAAKGLKAKPWVKTICAPGSQVVDGYYKRADLWKDLEALGFYLSGFGCTTCIGNSGPLPEEISAAINENDLTATAVLSGNRNFEGRISPDVKMNYLVSPIMVIAYAIAGTMDFDFETQPLGQDIDGNDVFLKDIWPSTEEIEETIAGAISRELYEADYADVFKGDEQWQNLPTPEGKTFDWDEKSTYIRKAPYFDGMTMEPAPVSDIKGARVLAKLGDSVTTDHISPASSIKPGTPAAQYLDENGVARNDYNSLGSRRGNHEVMMRGTFANIRLQNQLVD-IAGGYTRDFTKNGEQAFIFDACQNYKAAGIPLVVIAGKEYGTGSSRDWAAKGTNLLGVKAVITESFERIHRSNLIGMGVIPLQFPAGESHASLGLDGTETFDIEG---IEELNNGVTPKTVHVTATKESGDQVEFDAVVRIDTPGEADYYRNGGILQYVLRNMINA 934          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|75535948|sp|Q4UK20.1|ACNA_RICFE (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 994.186 bits (2569), Expect = 0.000e+0
Identity = 484/858 (56.41%), Postives = 624/858 (72.73%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAE--TFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGP-DGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGL-DTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNL 875
              KLP +L+V+ EN+LR  +G    S  ++  F EW  K  ++  EI + PARVLMQDFTGVPA+VDLAAMRD +  +GGD  +INPL PVDLVIDHSV +D +    +F  NV  E +RN+ERY FLKWGQ+AFNNF+VVPPGTGICHQVNLEYL++ VW    ++G  +AYPD+LVGTDSHTTMVNGL+VLGWGVGGIEAEAAMLGQP++M++PEV+G +LTG++    T TDLVL V EMLR   VVGKFVEF+G GL  L +ADRATI+NM+PEYGATCGFFPID ET++YL  TGR++ +I LVE YA E   W D ++   YT  L LD+  +  +++GP+RPQD V L +    F  E+       + + K+  V  ++Y + +G VVIA+ITSCTNTSNP VMIGA L+A+KA   GL  KPWVKTSLAPGS+VV+EYL+ +GL + LD++GFNLVGYGCTTCIGNSGP+  E+ + I +  LV  SVLSGNRNFEGRI+P  +A+YL SP LVVAYAL+GT++IDL  + +G+     +++LKDIWP+  EI+ ++  ++    FI+KY+D+F G ++W+ +Q   S TY+W   STYI NPPYF+ +      I ++  AK+LA+ GD +TTDHISPAGS   ++PA +YL +  +   +FNSYGSRRGNHEVMMRGTFANIRIKNEM  GVEGG+T       Q  I+DAAM ++A   P+VIFAG++YG+GSSRDWAAKG  LLGVKAVIAE+FERIHRSNLVGMG++P  FTG ++R  L L G ET+ I GL + I+P   V   I    G  + I +  +I T  EI YI++G ++H+V+ NL
Sbjct:   36 LKKLPYSLRVLFENVLR-SNG----SKQNLLVFKEWL-KTKESDAEIDFMPARVLMQDFTGVPAIVDLAAMRDAMKKIGGDPLKINPLIPVDLVIDHSVSVDSYAAKDSFDKNVQMEMKRNIERYQFLKWGQQAFNNFKVVPPGTGICHQVNLEYLAKVVW---HKDG--LAYPDSLVGTDSHTTMVNGLSVLGWGVGGIEAEAAMLGQPLTMILPEVIGVKLTGKLTGIATATDLVLTVTEMLRKKKVVGKFVEFFGEGLKNLTIADRATISNMSPEYGATCGFFPIDQETIKYLELTGREKTQIKLVEKYANEQNLWYDFEHEAEYTEILELDLSMVHSSLAGPRRPQDRVDLNDVANNFKHELPNFGIENKDIDKKYAVANQNYEIGNGDVVIAAITSCTNTSNPSVMIGAALLAKKALEHGLKVKPWVKTSLAPGSKVVTEYLKLSGLDKYLDELGFNLVGYGCTTCIGNSGPLNPEIEETINKNGLVVASVLSGNRNFEGRINPLTKASYLGSPILVVAYALSGTLNIDLNNQPIGK-----NIYLKDIWPSKEEIDEVIANSINSSMFIEKYSDIFSGTKEWKDLQITTSSTYNWNKNSTYINNPPYFEDIGSK-NNIKDIKSAKILAIFGDSITTDHISPAGSISKTSPAAKYLTDNHIEPLDFNSYGSRRGNHEVMMRGTFANIRIKNEMCKGVEGGFTINQLSSTQQTIYDAAMDYKANDVPVVIFAGKEYGSGSSRDWAAKGPQLLGVKAVIAESFERIHRSNLVGMGILPLTFTGNNTRLDLKLDGSETIDIIGLSEQIKPYNPVKCMIKKQTGETRTIDLILQIFTDNEINYIKHGSIMHFVVENL 876          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|81669260|sp|O53166.1|ACNA_MYCTU (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 993.03 bits (2566), Expect = 0.000e+0
Identity = 506/916 (55.24%), Postives = 645/916 (70.41%), Query Frame = 0

Query:   23 SKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREM-----------------------AETFRRPMGKEVTVVGED-------------------------------YTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKG---PDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTI---EPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKA 878
            +KLP +LKV+ EN+LR EDG  +++ D I+A + W D   +   EI Y PARV+MQDFTGVP +VDLA MR+ I  LGG+  ++NPL P DLVIDHSV+ D FG   AF+ NV+ EY+RN ERY FL+WGQ AF++F+VVPPGTGI HQVN+EYL+  V T   ++GV  AYPDT VGTDSHTTMVNGL VLGWGVGGIEAEAAMLGQPVSMLIP VVGF LTG++  G T TD+VL V EMLR HGVVGKFVEFYG G+  +PLA+RAT+ NM+PE+G+T   FPID ET++YLR TGR  +++ALVEAYAK  G W D  + P ++  L L++  +VP+I+GPKRPQD +AL  AK+ F  ++                        ETF      ++T   +D                               + ++ G VVIA+ITSCTNTSNP VM+GA L+AR A   GL  KPWVKT++APGSQVV++Y + +GL   L+K+GF LVGYGCTTCIGNSGP+ +E+S A+   DL  T+VLSGNRNFEGRI+PDV+ NYLASPPLV+AYALAGTMD D  T+ LGQD++G +VFL+DIWP+  +++  +   + +E F + YADVFKGD++W+ + TP   T++W   STY++ PPYF+GM+  P  + N+SGA+VLA+LGD VTTDHISPAG+ K  TPA +YL E  V  +++NS+GSRRGNHEVM+RGTFANIR++N++LD V GGYT+    P G QA I+DAA  + A+  PLV+F G++YG+GSSRDWAAKGT LLGV+AVIAE+FERIHRSNL+GMGVIP +F  G S  SLGL G E   I G+D +   +  + V    T  DG+  E     RIDT  E +Y  NGG+L YVLRN+ K+
Sbjct:   34 AKLPYSLKVLAENLLRNEDGS-NITKDHIEAIANW-DPKAEPSIEIQYTPARVVMQDFTGVPCIVDLATMREAIADLGGNPDKVNPLAPADLVIDHSVIADLFGRADAFERNVEIEYQRNGERYQFLRWGQGAFDDFKVVPPGTGIVHQVNIEYLASVVMT---RDGV--AYPDTCVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQPVSMLIPRVVGFRLTGEIQPGVTATDVVLTVTEMLRQHGVVGKFVEFYGEGVAEVPLANRATLGNMSPEFGSTAAIFPIDEETIKYLRFTGRTPEQVALVEAYAKAQGMWHDPKHEPEFSEYLELNLSDVVPSIAGPKRPQDRIALAQAKSTFREQIYHYVGNGSPDSPHDPHSKLDEVVEETFPASDPGQLTFANDDVATDETVHSAAAHADGRVSNPVRVKSDELGEFVLDHGAVVIAAITSCTNTSNPEVMLGAALLARNAVEKGLTSKPWVKTTIAPGSQVVNDYYDRSGLWPYLEKLGFYLVGYGCTTCIGNSGPLPEEISKAVNDNDLSVTAVLSGNRNFEGRINPDVKMNYLASPPLVIAYALAGTMDFDFQTQPLGQDKDGKNVFLRDIWPSQQDVSDTIAAAINQEMFTRNYADVFKGDDRWRNLPTPSGNTFEWDPNSTYVRKPPYFEGMTAKPEPVGNISGARVLALLGDSVTTDHISPAGAIKPGTPAARYLDEHGVDRKDYNSFGSRRGNHEVMIRGTFANIRLRNQLLDDVSGGYTRDFTQPGGPQAFIYDAAQNYAAQHIPLVVFGGKEYGSGSSRDWAAKGTLLLGVRAVIAESFERIHRSNLIGMGVIPLQFPEGKSASSLGLDGTEVFDITGIDVLNDGKTPKTVCVQATKGDGATIEFDAVVRIDTPGEADYYRNGGILQYVLRNILKS 942          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|81389935|sp|Q68VV0.1|ACNA_RICTY (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 991.49 bits (2562), Expect = 0.000e+0
Identity = 486/858 (56.64%), Postives = 619/858 (72.14%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMA--ETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGP-DGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLD-TIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNL 875
             +KLP +L+V+LEN+LR      S + +++  F EW  K  K+  EI + PARVLMQDFTGVPA+VDLAAMRD +  +G +  +INPL PVDLVIDHSV +D +G   +F+ NV  E +RN+ERY FLKWGQ+AFNNF+VVPPGTGICHQVNLEYLS+ VW +        AYPD+LVGTDSHTTMVNGL+VLGWGVGGIEAEAAMLGQP++M+IPEV+G +LTG++    T TDLVL + EMLR   VVGKFVEF+G GL  L ++DRATI+NM+PEYGATCGFFPID ET++YL  TGR+  +I LVE YA E   W + +    YT  L LD+ T+  +++GPKRPQD V L+  ++ F  E+       + + K+  V  ++Y + +G VVIA+ITSCTNTSNP VMIGA L+A+KA   GLN KPWVKTSLAPGS+VV+EYL+ +GL + LD +GFNLVGYGCTTCIGNSG +  E+ + I +  LV  SVLSGNRNFEGRI+P  +A+YLASP LVVAYAL+GT++IDL T  +G      +++LKDIWP+  EI+A++  ++    FI+KYADVF G ++W  +       Y+W   STYI NPPYF  +  +   I ++  AK+LA+ GD +TTDHISPAGS   ++PA +YLIE  +    FNSYGSRRGNHEVMMRGTFANIRIKNEM +GVEGG+T     G Q  I+DAAM +QA   PLV+FAG++YG+GSSRDWAAKG  LLG+KA+IAE+FERIHRSNLVGMG++P  FTG ++R SL L G ET+ I GL   I P   V   I         I +  +I T  EI YI++G ++ +V+ +L
Sbjct:   36 LNKLPYSLRVLLENVLRT-----SGNKENLLVFKEWL-KTKKSNTEIDFMPARVLMQDFTGVPAIVDLAAMRDAMQKIGCNPLKINPLIPVDLVIDHSVSVDSYGLKESFEQNVQMEMKRNIERYQFLKWGQQAFNNFKVVPPGTGICHQVNLEYLSKVVWYNDGT-----AYPDSLVGTDSHTTMVNGLSVLGWGVGGIEAEAAMLGQPLTMIIPEVIGVKLTGKLEGMATATDLVLTITEMLRRTKVVGKFVEFFGYGLSNLTISDRATISNMSPEYGATCGFFPIDQETIKYLEITGRETRQIKLVEKYATEQNLWYNFEDTQEYTEVLELDLSTVYSSLAGPKRPQDRVNLSFVESNFKNELPYFALENQDIDKKYAVANQNYEIGNGDVVIAAITSCTNTSNPSVMIGAALLAKKALEHGLNVKPWVKTSLAPGSKVVTEYLKISGLDKYLDALGFNLVGYGCTTCIGNSGSLNPEIENTINKNRLVVASVLSGNRNFEGRINPLTKASYLASPILVVAYALSGTLNIDLTTTPIGA-----NIYLKDIWPSQKEIDAVIANSINPSMFIEKYADVFNGTKEWHDLHITTGTNYNWDKNSTYINNPPYFDNICSE-NTIKDIKSAKILAIFGDSITTDHISPAGSISKNSPAAKYLIEHNIEPLNFNSYGSRRGNHEVMMRGTFANIRIKNEMCNGVEGGFTINQLSGVQQTIYDAAMDYQAHDIPLVVFAGKEYGSGSSRDWAAKGPGLLGIKAIIAESFERIHRSNLVGMGILPLTFTGNNTRLSLKLDGSETIDIIGLSKNIRPFNLVKCVIKKQTNEISTIDLILQIFTENEINYIKHGSIMQFVVESL 876          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|20137396|sp|Q92G90.1|ACNA_RICCN (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 989.178 bits (2556), Expect = 0.000e+0
Identity = 484/858 (56.41%), Postives = 623/858 (72.61%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETF--RRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGP-DGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGL-DTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNL 875
              KLP +L+V+ EN+LR      S+ V     F EW  K  K+  EI + PARVLMQDFTGVPA+VDLAAMRD +  +GGD  +INPL PVDLVIDHSV +D +    +F  NV  E +RN+ERY FLKWGQ+AFNNF+VVPPGTGICHQVNLEYL++ VW    ++G    YPD+LVGTDSHTTMVNGL+VLGWGVGGIEAEAAMLGQP++M++PEV+G +LTG++    T TDLVL V EMLR   VVGKFVEF+G GL  L +ADRATI+NM+PEYGATCGFFPID ET++YL  TGR++ +I LVE YA E   W D ++A  YT  L LD+  +  +++GPKRPQD V L +  + F  E+       + + K+  V  ++Y + +G VVIA+ITSCTNTSNP VMIGA L+A+KA   GL  KPWVKTSLAPGS+VV+EYL+ +GL + LD++GFNLVGYGCTTCIGNSGP+  E+ + I +  LV  SVLSGNRNFEGRI+P  +A+YL SP LVVAYAL+GT++IDL    +G+     +++LKD+WP+  EI+ ++  ++    FI+KY+D+F G ++W+ +Q  +S TY+W   STYI NPPYF+ +      I ++  AK+LA+LGD +TTDHISPAGS   ++PA +YL +  +   +FNSYGSRRGNHEVMMRGTFANIRIKNEM  GVEGG+T    +G Q  I+DAAM ++A    +VIFAG++YG+GSSRDWAAKG  LLGVKAVIAE+FERIHRSNLVGMG++P  FTG ++R  L L G ET+ I GL + I     V   I    G+ + I +  +I T  EI YI++G ++H+V+ +L
Sbjct:   36 LKKLPYSLRVLFENVLRSNGSKQSLLV-----FKEWL-KTKKSDAEIDFMPARVLMQDFTGVPAIVDLAAMRDAMKKIGGDPLKINPLIPVDLVIDHSVSVDSYAAKDSFNKNVQMEMKRNIERYAFLKWGQQAFNNFKVVPPGTGICHQVNLEYLAKVVW---HKDGT--LYPDSLVGTDSHTTMVNGLSVLGWGVGGIEAEAAMLGQPLTMILPEVIGVKLTGKLTGIATATDLVLTVTEMLRKKKVVGKFVEFFGEGLKNLMIADRATISNMSPEYGATCGFFPIDQETIKYLELTGREKTQIRLVEQYATEQNLWYDFEHAVEYTEVLELDLSMVHGSLAGPKRPQDRVNLNDVASNFKYELPNFALDNKDIDKKYAVANQNYEIGNGDVVIAAITSCTNTSNPSVMIGAALLAKKALEHGLKVKPWVKTSLAPGSKVVTEYLKLSGLDKYLDELGFNLVGYGCTTCIGNSGPLNPEIEETINKNGLVVASVLSGNRNFEGRINPLTKASYLGSPILVVAYALSGTLNIDLTNMPIGE-----NIYLKDLWPSKEEIDEVIANSINSSMFIEKYSDIFSGTKEWKDLQVTNSSTYNWNKNSTYINNPPYFKDIGSK-NNIQDIKSAKILAILGDSITTDHISPAGSISKTSPAAKYLTDHHIEPLDFNSYGSRRGNHEVMMRGTFANIRIKNEMCKGVEGGFTINQLNGTQQTIYDAAMDYKAHDVSVVIFAGKEYGSGSSRDWAAKGPGLLGVKAVIAESFERIHRSNLVGMGILPLTFTGNNTRLDLKLDGSETIDITGLSENISSYHPVKCVIKKQTGAIRTIDLILQIFTDNEINYIKHGSIMHFVVESL 876          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|6647436|sp|Q9ZCF4.1|ACNA_RICPR (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 980.704 bits (2534), Expect = 0.000e+0
Identity = 478/858 (55.71%), Postives = 614/858 (71.56%), Query Frame = 0

Query:   22 FSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMA--ETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGP-DGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGL-DTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNL 875
              KLP +L+V+LEN+LR          +++  F EW  K  K+  EI + PARVLMQDFTGVPA+VDLAAMRD +  +G +  +INPL PVDLVIDHSV +D +GN  +F  NV  E +RN+ERY FLKWGQ+AFNNF+VVPPGTGICHQVNLE+LSQ VW +        AYPD+LVGTDSHTTMVNGL+VLGWGVGGIEAE+AMLGQP++M+IPEV+G +L G++    T TDLVL + E+LR   VVGKFVEF+G GL  L ++DRATI+NM+PEYGATCGFFPID ETL+YL  TGR+  +I LVE YA E   W + +    YT  L LD+ T+  +++GPKRPQD V L    + F  E+       + + K+  V  ++Y + +G VVIA+ITSCTNTSNP VMIGA L+A+KA   GLN KPWVKTSLAPGS+VV+EYL+ +GL + LD +GFNLVGYGCTTCIGNSG +  E+ + I +  LV  SVLSGNRNFEGRI+P  +A+YLASP LVVAYAL+GT++IDL    +G      +++LKDIWP+  EI+ ++  ++    FI+KYADVF G ++W+ +Q      Y+W   STYI NPPYF  +  +   I ++  A++LA+ GD +TTDHISPAGS   ++PA +YLI+  +   +FNSYGSRRGNHEVMMRGTFANIRIKNEM +GVEGG+T     G Q  I+D AM ++A   PLVIFAG++YG+GSSRDWAAKG  LLG+KAVIAE+FERIHRSNLVGMG++P  FTG ++R +L L G E + + GL + I+P   V   I         I +  +I T  EI YI++G ++ +V+ +L
Sbjct:   36 LKKLPYSLRVLLENVLRTNGNK-----ENLLVFKEWL-KTKKSNTEIDFMPARVLMQDFTGVPAIVDLAAMRDAMQKIGCNPLKINPLIPVDLVIDHSVSVDSYGNKESFDQNVHMEMKRNIERYQFLKWGQQAFNNFKVVPPGTGICHQVNLEFLSQVVWHNDGT-----AYPDSLVGTDSHTTMVNGLSVLGWGVGGIEAESAMLGQPITMIIPEVIGVKLIGKLAGMATATDLVLTITEILRRKKVVGKFVEFFGNGLRNLTISDRATISNMSPEYGATCGFFPIDQETLKYLEVTGREITQIKLVEKYAIEQNLWYNCEDTQEYTEVLELDLSTVYSSLAGPKRPQDRVNLNCVASNFQNELPYFALANKDIDKKYAVANQNYAIGNGDVVIAAITSCTNTSNPSVMIGAALLAKKALEHGLNVKPWVKTSLAPGSKVVTEYLKLSGLDKYLDALGFNLVGYGCTTCIGNSGSLNPEIENTINKNRLVVASVLSGNRNFEGRINPLTKASYLASPILVVAYALSGTLNIDLTNTPIGA-----NIYLKDIWPSQKEIDEVIANSINSSMFIEKYADVFNGTKEWRDLQITTGTNYNWDKNSTYINNPPYFDNIGSEHS-IKDIKSARILAIFGDSITTDHISPAGSISKNSPAAKYLIKHHIEPLDFNSYGSRRGNHEVMMRGTFANIRIKNEMCNGVEGGFTINQLSGVQQTIYDTAMDYKAHDIPLVIFAGKEYGSGSSRDWAAKGPGLLGIKAVIAESFERIHRSNLVGMGILPLTFTGKNTRLNLKLDGSEIIDLIGLSENIKPYNLVKCVIKKQTNEISTIDLILQIFTENEINYIKHGSIMQFVVESL 876          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|81674773|sp|Q5HPJ0.1|ACNA_STAEQ (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 974.541 bits (2518), Expect = 0.000e+0
Identity = 481/873 (55.10%), Postives = 622/873 (71.25%), Query Frame = 0

Query:   15 EAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYA-PIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETF--------RRPMGKEVTVV---GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTK-GPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRN 874
            E  GL   SKLP +++V+LE++LR ED  F ++ D IKA S++ + G  N  E+ ++P+RV++QDFTGVPAVVDLA++R  +  +GGD  +INP  PVDLVIDHSV +D + NP A + N+  E+ERN ERY FL W  KAF+N+  VPP TGI HQVNLEYL+  V    D +G + A+PDTLVGTDSHTTM+NG+ VLGWGVGGIEAEA MLGQP    IPEV+G  LT  + +G+T TDL L+V E LR  GVVGKFVEF+G G+  LPLADRATIANMAPEYGATCGFFP+D E+L+Y++ TGRDE+ I LV+ Y ++N  + D +   P YT  + LD+ T+  ++SGPKRPQD + L++ K  F + +            +    K+  +    G   TM++G + IA+ITSCTNTSNPYVM+GAGLVA+KA   GL    +VKTSLAPGS+VV+ YL  +GLQ+ LD +GFNLVGYGCTTCIGNSGP+  E+  A+A  DL+ TSVLSGNRNFEGRI P V+ANYLASP LVVAYALAGT+DIDL  E +G+ ++G DV+L+DIWP+  E++  V+K VT E F+++Y +V+  +E W  +   D   YD+   STYIQNP +FQG+SK+PGKI  L   +V+   GD VTTDHISPAG+    TPAG+YL++  V +R FNSYGSRRGNHEVM+RGTFANIRIKN++  G EGG+T   P G    I+DAAM ++ +GT LV+ AG  YG GSSRDWAAKGT LLGVK VIA+++ERIHRSNLV MGV+P +F  G+S ++LGL G E +++   + ++P   V  T    +G         R D+ VE++Y  +GG+L  VLRN
Sbjct:   29 EEKGLAKISKLPYSIRVLLESVLRQEDD-FVITDDHIKALSKFGNAG--NEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMNDVGGDINKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLANVVHV-RDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLTHSLPQGSTATDLALRVTEELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDEESLKYMKLTGRDEEHIELVKEYLQQNHMFFDVEKEDPEYTDVIDLDLSTVEASLSGPKRPQDLIFLSDMKKEFEKSVTAPAGNQGHGLDQSEFDKKAEINFNDGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEFVKTSLAPGSKVVTGYLRDSGLQQYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAVADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGTVDIDLQNEPIGKGKDGKDVYLQDIWPSIQEVSDTVDKVVTPELFLEEYKNVYHNNEMWNEIDVTDEPLYDFDPNSTYIQNPTFFQGLSKEPGKIEPLKSLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLLDHDVAIRNFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYWPTGEIMPIYDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLQFQQGESAEALGLDGKEEISVDINEDVQPHDFVNVTAKKENGEIINFKAIVRFDSLVELDYYRHGGILQMVLRN 897          

BLAST of aconitate hydratase 1 vs. SwissProt
Match: gi|38604821|sp|Q8CPC2.1|ACNA_STAES (RecName: Full=Aconitate hydratase A; Short=ACN; Short=Aconitase; AltName: Full=(2R,3S)-2-methylisocitrate dehydratase; AltName: Full=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; AltName: Full=Iron-responsive protein-like; Short=IRP-like; AltName: Full=Probable 2-methyl-cis-aconitate hydratase; AltName: Full=RNA-binding protein)

HSP 1 Score: 974.156 bits (2517), Expect = 0.000e+0
Identity = 481/873 (55.10%), Postives = 622/873 (71.25%), Query Frame = 0

Query:   15 EAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYA-PIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETF--------RRPMGKEVTVV---GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTK-GPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRN 874
            E  GL   SKLP +++V+LE++LR ED  F ++ D IKA S++ + G  N  E+ ++P+RV++QDFTGVPAVVDLA++R  +  +GGD  +INP  PVDLVIDHSV +D + NP A + N+  E+ERN ERY FL W  KAF+N+  VPP TGI HQVNLEYL+  V    D +G + A+PDTLVGTDSHTTM+NG+ VLGWGVGGIEAEA MLGQP    IPEV+G  LT  + +G+T TDL L+V E LR  GVVGKFVEF+G G+  LPLADRATIANMAPEYGATCGFFP+D E+L+Y++ TGRDE+ I LV+ Y ++N  + D +   P YT  + LD+ T+  ++SGPKRPQD + L++ K  F + +            +    K+  +    G   TM++G + IA+ITSCTNTSNPYVM+GAGLVA+KA   GL    +VKTSLAPGS+VV+ YL  +GLQ+ LD +GFNLVGYGCTTCIGNSGP+  E+  A+A  DL+ TSVLSGNRNFEGRI P V+ANYLASP LVVAYALAGT+DIDL  E +G+ ++G DV+L+DIWP+  E++  V+K VT E F+++Y +V+  +E W  +   D   YD+   STYIQNP +FQG+SK+PGKI  L   +V+   GD VTTDHISPAG+    TPAG+YL++  V +R FNSYGSRRGNHEVM+RGTFANIRIKN++  G EGG+T   P G    I+DAAM ++ +GT LV+ AG  YG GSSRDWAAKGT LLGVK VIA+++ERIHRSNLV MGV+P +F  G+S ++LGL G E +++   + ++P   V  T    +G         R D+ VE++Y  +GG+L  VLRN
Sbjct:   29 EEKGLAKISKLPYSIRVLLESVLRQEDD-FVITDDHIKALSKFGNAG--NEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMNDVGGDINKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLANVVHV-RDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLTHSLPQGSTATDLALRVTEELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDEESLKYMKLTGRDEEHIELVKEYLQQNHMFFDVEKEDPEYTDVIDLDLSTVEASLSGPKRPQDLIFLSDMKKEFEKSVTAPAGNQGHGLDQSEFDKKAEINFNDGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEFVKTSLAPGSKVVTGYLRDSGLQQYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAVADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGTVDIDLQNEPIGKGKDGKDVYLQDIWPSIQEVSDTVDKVVTPELFLEEYKNVYHNNEMWNEIDVTDEPLYDFDPNSTYIQNPTFFQGLSKEPGKIEPLKSLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLLDHDVAIRNFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYWPTGEIMPIYDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLQFQQGESAEALGLDGKEEISVDINEDVQPHDLVNVTAKKENGEIINFKAIVRFDSLVELDYYRHGGILQMVLRN 897          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|640620674|ref|WP_025048722.1| (aconitate hydratase AcnA [Sulfitobacter mediterraneus] >gi|751956422|gb|KIN77065.1| Aconitate hydratase 1 [Sulfitobacter mediterraneus KCTC 32188])

HSP 1 Score: 1592.79 bits (4123), Expect = 0.000e+0
Identity = 768/895 (85.81%), Postives = 821/895 (91.73%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKA 878
            MT+TVG DTAKT                  A+ AGLGDFSKLPAALKVVLENMLRFEDG  +V+VDDIKAF+EW  KGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDG+VALGGDA++INPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYL+QTVWTDTDQNG E+AYPDTLVGTDSHTTMVNG+AVLGWGVGGIEAEAAMLGQP+SMLIPEVVGFELTG MMEGTTGTDLVLKVVEMLR  GVVGKFVEFYGAGLD LPLADRATIANMAPEYGATCGFFPIDGETLRY+R TGRDEDRIALVEAYAKENG WR  DYAP+YT TLSLDMGTIVPAISGPKRPQDYVALTNAK AF  EMA TF+RPMGK+V V GEDYT+ESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGL+RKPWVKTSLAPGSQVVS YLEAAGLQ+DLDKIGFNLVGYGCTTCIGNSGP+Q E+S A+ +GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDI+LAT+ + Q  +G DV+LKDIWPT+ E+  LVE+TVTREAF  KYADVFKGDEKWQAV+T DS+TYDWP  STY+QNPPYFQGMSK+PG I+N+ GAKVLAVLGDM+TTDHISPAGSFK  TPAGQYL ERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQ +IFDAAMAHQA GTPLV+F GEQYGAGSSRDWAAKGTALLGVKAVI+E+FERIHRSNLVGMGVIPFEFT GDS KSLGLTG+ETV+I+GLDTI+PLQ V   ITMADG+ K I +KCRIDTA+EIEYIE+GGVLHYVLR+L+KA
Sbjct:    1 MTITVGQDTAKTRKSITAGSQKISYYSIPAAQDAGLGDFSKLPAALKVVLENMLRFEDGK-TVTVDDIKAFAEWGAKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGLVALGGDAEKINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLAQTVWTDTDQNGEEVAYPDTLVGTDSHTTMVNGMAVLGWGVGGIEAEAAMLGQPISMLIPEVVGFELTGAMMEGTTGTDLVLKVVEMLREKGVVGKFVEFYGAGLDSLPLADRATIANMAPEYGATCGFFPIDGETLRYMRTTGRDEDRIALVEAYAKENGMWRGDDYAPVYTDTLSLDMGTIVPAISGPKRPQDYVALTNAKAAFADEMANTFKRPMGKQVAVDGEDYTLESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLDRKPWVKTSLAPGSQVVSAYLEAAGLQDDLDKIGFNLVGYGCTTCIGNSGPLQPEISKAVNEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDINLATDPIAQTPDGKDVYLKDIWPTTQEVAELVEQTVTREAFQTKYADVFKGDEKWQAVETTDSQTYDWPVQSTYVQNPPYFQGMSKEPGVITNIEGAKVLAVLGDMITTDHISPAGSFKADTPAGQYLTERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQTSIFDAAMAHQANGTPLVVFGGEQYGAGSSRDWAAKGTALLGVKAVISESFERIHRSNLVGMGVIPFEFTNGDSCKSLGLTGEETVSISGLDTIQPLQEVPCAITMADGTVKNIMIKCRIDTAIEIEYIEHGGVLHYVLRDLAKA 894          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|1189783464|ref|WP_085820904.1| (aconitate hydratase AcnA [Ruegeria meonggei] >gi|1188037019|emb|SLN14421.1| Aconitate hydratase 1 [Ruegeria meonggei])

HSP 1 Score: 1592.4 bits (4122), Expect = 0.000e+0
Identity = 764/895 (85.36%), Postives = 816/895 (91.17%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKA 878
            M + VG DTAKT                  A+ AGLGDFSKLPAALKVVLENMLRFED GFSVSVDDIKAF+EW  KGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGI  LGGDAQ+INPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERY FLKWGQ AFNNFRVVPPGTGICHQVNLEYL+QTVWTDTDQNG E+AYPDTLVGTDSHTTMVNG+AVLGWGVGGIEAEAAMLGQP+SMLIPEVVGFELTG ++EGTTGTDLVLKVVEMLR  GVVGKFVEFYG GLD LPLADRATIANMAPEYGATCGFFPIDGETLRY+RNTGRDEDRIALVEAYAKENGFWR ADYAP+YT TLSLDMGTIVPAISGPKRPQDYVALT+AKTAFT+EM ETF+RPMGKEV V GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVS YLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQ ELS+AIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVV YALAGT+DIDL +E +GQD++GNDV++KDIWPT+ E+  LVE+TVTREAF  KYADVFKGD+KWQ V+ P  ETYDWP  STYIQNPPYFQGM  +PG ISN+ GAKVL +LGDM+TTDHISPAGSF  +TPAGQYLI+RQV  REFNSYGSRRGNHE+MMRGTFANIR+KNEMLDGVEGGYTKGPDG Q +++DA+MA+Q +GTPLV+F GEQYGAGSSRDWAAKGTALLGVKAVIAE+FERIHRSNLVGMGVIPFEFTGGD+RKSLGLTGDETV+I GLDTI+PLQ V  TI   DG+ K IT+KCRIDTA EIEYIENGGVLHYVLRNL+KA
Sbjct:    1 MPIKVGQDTAKTRRSLSVNGKSISYYSIPAAQEAGLGDFSKLPAALKVVLENMLRFEDDGFSVSVDDIKAFAEWGAKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGINGLGGDAQKINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYQFLKWGQNAFNNFRVVPPGTGICHQVNLEYLAQTVWTDTDQNGDEVAYPDTLVGTDSHTTMVNGMAVLGWGVGGIEAEAAMLGQPISMLIPEVVGFELTGALLEGTTGTDLVLKVVEMLREKGVVGKFVEFYGEGLDRLPLADRATIANMAPEYGATCGFFPIDGETLRYMRNTGRDEDRIALVEAYAKENGFWRGADYAPVYTDTLSLDMGTIVPAISGPKRPQDYVALTDAKTAFTKEMEETFKRPMGKEVAVKGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSAYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQAELSEAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVVYALAGTLDIDLTSEPIGQDKDGNDVYMKDIWPTTQEVAELVEQTVTREAFQSKYADVFKGDKKWQEVEVPQQETYDWPPTSTYIQNPPYFQGMGAEPGTISNIDGAKVLLILGDMITTDHISPAGSFATTTPAGQYLIDRQVQPREFNSYGSRRGNHEIMMRGTFANIRVKNEMLDGVEGGYTKGPDGQQTSVYDASMAYQEQGTPLVVFGGEQYGAGSSRDWAAKGTALLGVKAVIAESFERIHRSNLVGMGVIPFEFTGGDTRKSLGLTGDETVSIHGLDTIQPLQEVPCTIVYGDGTEKTITLKCRIDTAPEIEYIENGGVLHYVLRNLAKA 895          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|740161307|ref|WP_038004817.1| (MULTISPECIES: aconitate hydratase AcnA [Thalassobacter] >gi|695756892|gb|KGK80872.1| aconitate hydratase [Thalassobacter stenotrophicus] >gi|695956654|gb|KGL02257.1| aconitate hydratase [Thalassobacter sp. 16PALIMAR09])

HSP 1 Score: 1590.47 bits (4117), Expect = 0.000e+0
Identity = 764/896 (85.27%), Postives = 823/896 (91.85%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            MTV VG+DTA+T                  A+AAGLGDFS+LPAALKVVLENMLRFEDGG +VSVDDIKAF++WA +GG+NPREI YRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERYTFLKWGQ AFNNFRVVPPGTGICHQVNLEYL+QTVW+D DQNGV+IAYPDTLVGTDSHTTMVNG+AVLGWGVGGIEAEAAMLGQP+SMLIPEVVGFELTGQM EGTTGTDLVLKVVEMLRA GVVGKFVEFYGAGLD LPLADRATIANMAPEYGATCGFFPID ETLRY+R TGRD DRIALVEAYAK NG WRDA+Y+PIYT TL LDMGTIVPAISGPKRPQDYVALT+AK+AFTREMAETF+RP  KEV V GE+Y++ SGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVS YLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGP+Q E+S+AIA GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDL TE LGQDQ G DVFL+DIWPT AEI  LVE+TVTREAF  KYADVFKGDEKWQ+V+T D +TYDWP  STY+QNPPYFQGM  +PG I N+ GAKVLAVLGDM+TTDHISPAG+FK++TPAG+YL++RQVP+REFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDG Q +IFDAAMA+++ GTPLVIF G QYGAGSSRDWAAKGTALLGVKAVIAE+FERIHRSNLVGMGVIP EFTG D+RK+LGLTG+ETV+I GLDT++PLQ V  TITM+DGS KEIT++CRIDT VEIEYIENGGVLHYVLRNL+KA+
Sbjct:    1 MTVVVGSDTAQTRKTLNIGAAQIDYYSIPAAQAAGLGDFSRLPAALKVVLENMLRFEDGGRTVSVDDIKAFAQWAVQGGQNPREINYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYTFLKWGQSAFNNFRVVPPGTGICHQVNLEYLAQTVWSDADQNGVQIAYPDTLVGTDSHTTMVNGMAVLGWGVGGIEAEAAMLGQPISMLIPEVVGFELTGQMREGTTGTDLVLKVVEMLRAKGVVGKFVEFYGAGLDTLPLADRATIANMAPEYGATCGFFPIDAETLRYMRTTGRDADRIALVEAYAKANGLWRDANYSPIYTETLHLDMGTIVPAISGPKRPQDYVALTDAKSAFTREMAETFQRPTDKEVPVQGENYSLSSGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSAYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPLQPEISEAIAAGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLTTEPLGQDQEGRDVFLRDIWPTQAEIAELVEQTVTREAFQTKYADVFKGDEKWQSVETTDQKTYDWPPNSTYVQNPPYFQGMGMEPGTIENVEGAKVLAVLGDMITTDHISPAGAFKETTPAGRYLMDRQVPLREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGTQMSIFDAAMAYKSSGTPLVIFGGAQYGAGSSRDWAAKGTALLGVKAVIAESFERIHRSNLVGMGVIPLEFTGEDTRKTLGLTGEETVSIHGLDTVKPLQEVPCTITMSDGSIKEITLRCRIDTEVEIEYIENGGVLHYVLRNLAKAS 896          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|653006999|ref|WP_027259168.1| (aconitate hydratase AcnA [Leisingera aquimarina])

HSP 1 Score: 1588.55 bits (4112), Expect = 0.000e+0
Identity = 764/895 (85.36%), Postives = 819/895 (91.51%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKA 878
            M +TVG D AK                   A  AG GDFSKLPAALKVVLENMLRFEDGGFSVS DDIKAF+EW   GGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIV L G A +INPL PVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERY FLKWGQ AF+NFRVVPPGTGICHQVNLEYLSQTVWTDTDQNG E+AYPDTLVGTDSHTTMVNG AVLGWGVGGIEAEAAMLGQP+SMLIPEV+GFELTGQM+EGTTGTDLVLKVVEMLRA GVVGKFVEFYG GLD LPLADRATIANMAPEYGATCGFFPID ETLRYLRNTGRDEDRIALVEAYAKENGFWR ADY+P+YT TLSLDMG+IVPAISGPKRPQDYVALT AK+AF++EM ETF+RPMGKEV V GEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGL+RKPWVKTSLAPGSQVVS YLEAAGLQEDLDK+GFNLVGYGCTTCIGNSGPIQ+E+S+AIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDI+LA++ + QD++GNDV+LKDIWP++ E+  LVE+TVTREAF  KYADVFKGDEKWQAV+T D+ETYDWP+ASTYIQNPPYFQGM  +PG ISN+  AKVLAVLGDMVTTDHISPAGSF  +TPAGQYL+ERQV  REFNSYGSRRGNHE+MMRGTFANIRIKNEMLDGVEGGYTKGPDG Q +IFDA+MAHQ  GTPLV+F GEQYGAGSSRDWAAKGTALLGVKAVIAE+FERIHRSNLVGMGVIPFEFTGGDSRKSLGLTG+ETV+I+GLD+I+PLQ+V   ITMADGS KEIT+KCRIDTA EIEYIE+GGVLHYVLRNL+K+
Sbjct:    1 MPITVGQDNAKARKTLSVNGKSISYYSIPAATEAGFGDFSKLPAALKVVLENMLRFEDGGFSVSTDDIKAFAEWGALGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVGLKGSAAKINPLVPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYQFLKWGQNAFDNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGEEVAYPDTLVGTDSHTTMVNGAAVLGWGVGGIEAEAAMLGQPISMLIPEVIGFELTGQMVEGTTGTDLVLKVVEMLRAKGVVGKFVEFYGTGLDRLPLADRATIANMAPEYGATCGFFPIDNETLRYLRNTGRDEDRIALVEAYAKENGFWRGADYSPVYTDTLSLDMGSIVPAISGPKRPQDYVALTGAKSAFSKEMEETFKRPMGKEVAVKGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLDRKPWVKTSLAPGSQVVSAYLEAAGLQEDLDKVGFNLVGYGCTTCIGNSGPIQQEISEAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDINLASDPIAQDKDGNDVYLKDIWPSTQEVAELVEQTVTREAFQSKYADVFKGDEKWQAVETTDAETYDWPSASTYIQNPPYFQGMGTEPGTISNIKDAKVLAVLGDMVTTDHISPAGSFATTTPAGQYLLERQVQPREFNSYGSRRGNHEIMMRGTFANIRIKNEMLDGVEGGYTKGPDGEQTSIFDASMAHQEAGTPLVVFGGEQYGAGSSRDWAAKGTALLGVKAVIAESFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGEETVSISGLDSIQPLQDVPCAITMADGSVKEITLKCRIDTAPEIEYIEHGGVLHYVLRNLAKS 895          

BLAST of aconitate hydratase 1 vs. nr
Match: gi|1189758980|ref|WP_085803683.1| (aconitate hydratase AcnA [Roseovarius albus] >gi|1188019820|emb|SLN11969.1| Aconitate hydratase 1 [Roseovarius albus])

HSP 1 Score: 1587.78 bits (4110), Expect = 0.000e+0
Identity = 769/896 (85.83%), Postives = 823/896 (91.85%), Query Frame = 0

Query:    1 MTVTVGTDTAKT-----------------PAEAAGLGDFSKLPAALKVVLENMLRFEDGGFSVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNLERYTFLKWGQKAFNNFRVVPPGTGICHQVNLEYLSQTVWTDTDQNGVEIAYPDTLVGTDSHTTMVNGLAVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFELTGQMMEGTTGTDLVLKVVEMLRAHGVVGKFVEFYGAGLDVLPLADRATIANMAPEYGATCGFFPIDGETLRYLRNTGRDEDRIALVEAYAKENGFWRDADYAPIYTATLSLDMGTIVPAISGPKRPQDYVALTNAKTAFTREMAETFRRPMGKEVTVVGEDYTMESGKVVIASITSCTNTSNPYVMIGAGLVARKAAALGLNRKPWVKTSLAPGSQVVSEYLEAAGLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSDAIAQGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDIDLATEALGQDQNGNDVFLKDIWPTSAEINALVEKTVTREAFIKKYADVFKGDEKWQAVQTPDSETYDWPAASTYIQNPPYFQGMSKDPGKISNLSGAKVLAVLGDMVTTDHISPAGSFKDSTPAGQYLIERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGAQAAIFDAAMAHQAEGTPLVIFAGEQYGAGSSRDWAAKGTALLGVKAVIAENFERIHRSNLVGMGVIPFEFTGGDSRKSLGLTGDETVAIAGLDTIEPLQNVTATITMADGSNKEITVKCRIDTAVEIEYIENGGVLHYVLRNLSKAA 879
            M +TVG DTAKT                  AE AGLG+FS+LPAAL+VVLENMLRFEDG  +VSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERN+ERY FLKWGQ AFNNFRVVPPGTGICHQVNLEYL+QTVW+DTDQNG  +AYPDTLVGTDSHTTMVNG+AVLGWGVGGIEAEAAMLGQP+SMLIPEVVGFELTG M+EGTTGTDLVLKVVE+LR  GVVGKFVEFYG GLD LPLADRATIANMAPEYGATCGFFPID ETLRYLR+TGRD+DRIALVEAYAKENGFWR ADYAPIYT TL LDMGTIVPAISGPKRPQDYVALT+AK+AFTREM ETF+RPM KEV V GEDYT+ SG VVIASITSCTNTSNPYVMIGAGLVARKAAALGL+RKPWVKTSLAPGSQVVS YLEAA LQEDLDKIGFNLVGYGCTTCIGNSGPIQKELS+AIA+GDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDI+LAT+ + Q  +G DV+LKDIWP++ EI  LVE+TVTREAF  KYADVFKGD+KWQ+V+T + ETYDWP  STY+QNPPYFQGMSK+PG I+N+ GA+VLA+LGDMVTTDHISPAGSFKD+TPAGQYL ERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDG++ +IFDAAMA+QA GTPLV+F GEQYGAGSSRDWAAKGTALLGVKAVIAE+FERIHRSNLVGMGVIPFEFTGGDSR SLGLTG ETVAI+GLDTI+PLQ V  TITMADG+ K I +KCRIDTA+EIEYIE+GGVLHYVLRNL+ AA
Sbjct:    1 MPITVGHDTAKTRKTLSVNRQSVSYYSIAAAEEAGLGNFSRLPAALRVVLENMLRFEDGK-TVSVDDIKAFSEWADKGGKNPREIAYRPARVLMQDFTGVPAVVDLAAMRDGIVALGGDAQQINPLNPVDLVIDHSVMIDEFGNPRAFQMNVDREYERNMERYQFLKWGQGAFNNFRVVPPGTGICHQVNLEYLAQTVWSDTDQNGDMVAYPDTLVGTDSHTTMVNGMAVLGWGVGGIEAEAAMLGQPISMLIPEVVGFELTGAMLEGTTGTDLVLKVVELLRERGVVGKFVEFYGEGLDRLPLADRATIANMAPEYGATCGFFPIDDETLRYLRSTGRDDDRIALVEAYAKENGFWRGADYAPIYTDTLHLDMGTIVPAISGPKRPQDYVALTDAKSAFTREMEETFKRPMDKEVAVEGEDYTLSSGDVVIASITSCTNTSNPYVMIGAGLVARKAAALGLDRKPWVKTSLAPGSQVVSAYLEAANLQEDLDKIGFNLVGYGCTTCIGNSGPIQKELSEAIAEGDLVATSVLSGNRNFEGRISPDVRANYLASPPLVVAYALAGTMDINLATDPIAQTPDGKDVYLKDIWPSTQEIAELVEQTVTREAFQSKYADVFKGDDKWQSVETSEGETYDWPPQSTYVQNPPYFQGMSKEPGTITNIEGARVLALLGDMVTTDHISPAGSFKDTTPAGQYLTERQVPVREFNSYGSRRGNHEVMMRGTFANIRIKNEMLDGVEGGYTKGPDGSETSIFDAAMAYQAAGTPLVVFGGEQYGAGSSRDWAAKGTALLGVKAVIAESFERIHRSNLVGMGVIPFEFTGGDSRSSLGLTGAETVAISGLDTIQPLQEVPCTITMADGTEKTIQLKCRIDTAIEIEYIEHGGVLHYVLRNLAGAA 895