BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000007008 (supercontig:LSalAtl2s:LSalAtl2s398:345702:357490:1 gene:EMLSAG00000007008 transcript:EMLSAT00000007008 description:"maker-LSalAtl2s398-augustus-gene-3.18")

HSP 1 Score: 1366.29 bits (3535), Expect = 0.000e+0
Identity = 648/751 (86.28%), Postives = 703/751 (93.61%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQD 1014
            RR+ECPPHIFGVAEGSYQGM+N  KNQSILITGESGAGKTENTKKVISYFAS+GASGK+KEGE  LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMV+YLLEKSRLTFQAELERCYHAFYN+MSD VPDLK+ CLLS++IYDYWW SQGKV+V SIDDKEDMQ+A +A+ ILGFN+EE YNVYKLT+VVMHMGNMTKDFVPVGKEEQAEIK + N++KVA +CGIDSEWMI YFCKPKLKVGTEWV+KGQTCS A+SSVAGI RKIYEL FRFI +KCNETLFDP MKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCI MFEKPMGLL+ILEEESLFPKATDQ+FAAKLHE+LL KC NFQKA+P+PDP+AHFAV+HYAA VSYNLT WLEKNKDPLNDT+VELFKNGSN LL+ECFRDHPGQPLEAKKD+ GGGRKKGGGKTVSSFYK QLDDLMKTLYATDP+FIRCVVPNTHK+PGGVE+GLVMHQYQCNG LAGIAICRKGFPNKM Y +FK RYNILAA AVAKAK DK+AA AVL  +KLE EKFRLGHTKVFFRAGILGYMEE+RED++G VLSWLQ+QARGK+SR+VFKKMQDQKLALY CQRTIRN++IGKTWLWWQ+WL +KPNLKCTKF+QYKAEYEEKIAIAE NI KAL+DRKKVE ++  +L QK+EL LAL+SGGSAVQD
Sbjct:  183 RRNECPPHIFGVAEGSYQGMLNACKNQSILITGESGAGKTENTKKVISYFASIGASGKKKEGEVGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVVYLLEKSRLTFQAELERCYHAFYNIMSDCVPDLKENCLLSNDIYDYWWXSQGKVTVPSIDDKEDMQFADEAYDILGFNKEEKYNVYKLTAVVMHMGNMTKDFVPVGKEEQAEIKDDANSIKVASLCGIDSEWMITYFCKPKLKVGTEWVSKGQTCSGAASSVAGIGRKIYELAFRFIVEKCNETLFDPVMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCITMFEKPMGLLSILEEESLFPKATDQTFAAKLHEHLLGKCENFQKANPRPDPNAHFAVIHYAAVVSYNLTGWLEKNKDPLNDTIVELFKNGSNKLLVECFRDHPGQPLEAKKDS-GGGRKKGGGKTVSSFYKTQLDDLMKTLYATDPAFIRCVVPNTHKQPGGVEAGLVMHQYQCNGXLAGIAICRKGFPNKMVYPEFKNRYNILAAQAVAKAKNDKNAAAAVLKAIKLEGEKFRLGHTKVFFRAGILGYMEEIREDKIGAVLSWLQAQARGKSSRLVFKKMQDQKLALYCCQRTIRNWHIGKTWLWWQIWLALKPNLKCTKFAQYKAEYEEKIAIAEANIDKALSDRKKVEAVNSAILNQKNELVLALQSGGSAVQD 932          

HSP 2 Score: 515.383 bits (1326), Expect = 4.136e-159
Identity = 233/263 (88.59%), Postives = 248/263 (94.30%), Query Frame = 0

Query:    1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            MPG+IKKS+GPDPDP  WLIVS ELK KLKSKPYD KKSCWVP+K++GGY EGLI STDGDKVTV +L + D KVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLW+SVVRYKNELIYTYSGLFCIAINPYKRFPIYTQR++D+YIGKRR+ECPPHIFGVAEGSYQGM+N  KNQSILITGESGAGKTENTKKVISYFAS+GASGK+KEGE  LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM
Sbjct:   33 MPGNIKKSEGPDPDPDQWLIVSDELKIKLKSKPYDAKKSCWVPEKSTGGYLEGLIQSTDGDKVTVKLLGSDDTKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWBSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRAMDIYIGKRRNECPPHIFGVAEGSYQGMLNACKNQSILITGESGAGKTENTKKVISYFASIGASGKKKEGEVGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 295          

BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000007113 (supercontig:LSalAtl2s:LSalAtl2s400:60933:72707:1 gene:EMLSAG00000007113 transcript:EMLSAT00000007113 description:"maker-LSalAtl2s400-augustus-gene-0.11")

HSP 1 Score: 867.84 bits (2241), Expect = 0.000e+0
Identity = 582/1633 (35.64%), Postives = 907/1633 (55.54%), Query Frame = 0

Query:  451 ILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKP---------------DPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAA-AAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKI-----AIAEKNI----------------GKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARAL-----NEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTEL-ESSR-----ERIENEHER-KSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKSPIRI 2034
            ILGF+ EE  + YK+ + VMH+G M   F   G+EEQAE   E +   V ++ G++S  + +   +PK+KVGTE+VT+G+   Q   SV+ + + I++ +F+++  KCN ++ +  +KK  +IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY REGI W  +DFG+DLQ CID+ EKP+G+ +ILEEES+FPKAT++SF  KL  N + K PNF    PK                DP+ HFA++HYA TV YN+  WLEKNKDPLND VV+  +N  N    E F+DH G+    K+  G         +TVS+ Y+ Q+ +LM+ L AT P FIRC++PN  K PG +++ LVM Q  CNGVL GI ICRKGFPN+M Y DFK RY ILA+ A +A    +K AAK     + LE +K+RLGH+KVFFR+G+LG++EE+R+ ++ E++S+LQ+  RG   ++   +   +K AL   QR IRN+   + W+W++L+  I+P L+         E  EK+     A+ E+ I                 K L + K+ + + + +  ++ EL  A +    A+QD + K    E                            Q + + Q +  +L+EE++ M+  LA   QD+  K +Q+  LK+ELE Q E + +L +EK+ + +     +E+++  E+++ HL K+K K+E  +D+ E++L+ EK+ K   EK KRK+E DLK     I+D E  +  L    QRKEKE AA+ AKIDD      K+ K  ++   R+E+L+EEL  E+  R++ E+ R  L                            +QEL  +K +++E      G  + ++ + N                  K E D  +M +DL+EA+   E                  ++  A  K D+    L     N  +S+ K LE +K+DLE  +     A+AQ  + +I       D   L     RDRS+L+       T+L E++R     E+I+N  E+ K+D L+ L  A+ +I                                      LN+  ++ EKS+ R+ ++LE++  +YE +       EK   NFD+ + EWK K ++L   +  S KE R Y++EL  ++  + +          E K L +EI++LL Q+GDGGR+  EL  Q+R++ +E++ELQA L+EAE+ALE EENK+  S+LE  +++ +++ +I++KE+EF +++K   R + SL AS  AEQ+ K E  R+KK+LE DI ELE+ALD +N  N E  +  KR   Q  +  +  E+E   ++EV+E + + +R+ N+L  E+E+ +  +  ++R++RQL+ E+++ +G ++  S         KR  E+ I +++AE+ D     K+SE K ++A++D   L+++L++  +    E+  +R LE QL E+   L + EA++ + G+  + KLE RI+ LE +L + +    ++ K  +++ER VK+ + Q EE  ++  R  ++   L  KI+  KQQIEEAEEIAALNLAKF+K+  E  +SE R+  AE++  ++
Sbjct:    3 ILGFSAEEKMDTYKIVASVMHLGKMK--FKQRGREEQAEADGEQSGNTVCKLMGMNSNELFDNLLRPKIKVGTEFVTQGRNKKQVCYSVSALCKAIFDRLFKWLXFKCNASM-ETQIKKHHFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGISWTFIDFGLDLQACIDLIEKPLGIFSILEEESMFPKATNKSFEEKLINNHMGKTPNF--IRPKQTGHGKGGSVVHDSDEDPNPHFAIIHYAGTVPYNVNGWLEKNKDPLNDCVVDQIRNAKNQRARELFKDHAGEQKHTKRTRGSAF------QTVSAIYREQVQNLMQXLXATSPHFIRCIIPNESKDPGKIDAMLVMQQLTCNGVLEGIRICRKGFPNRMFYKDFKERYLILASKAMLAFGNDEKMAAKMCFAKIALEDDKYRLGHSKVFFRSGVLGFLEELRDAKLSEMISYLQAACRGYLGKLRIAEYIRRKKALPVVQRAIRNFIKIRPWMWYRLFKTIRPMLEMGVSEDIIHELREKLEAQRLAMNEEEIRITEVEEELEKAEKEKNKLLDEMKEQQRILDEIKAKEEELKEAARRREQAIQDALRKAEEEEEAKY---------------------EAEQAKKRAQMEVRKLKEELDDMKEALAKFGQDRDSKSQQLFALKDELERQNEQMNRLNKEKKDLYNQNNSIQEELETSEERNRHLEKLKKKMESEIDDLENSLQKEKEAKLQMEKYKRKVEEDLKKYMADIADQEEAQKSLMTLNQRKEKEMAALMAKIDDNEAXCAKHQKMXRDXAMRIEDLEEELEAEKKYRSQTEQHRRNL----------------------------QQELDDLKLKLDE-----AGDHSVIQAEFNR-----------------KRESDIQSMRKDLEEAKIKQE-----------------VALAVARKKYDQALCGLRDECDNHLQSRTK-LEKDKIDLENSLR---GAVAQVERGRI-------DKANLERELKRDRSTLI----EFKTKLDEANRYAFDFEQIKNRQEKDKNDLLRRLEDAENQI-------------------------------------GLNSHCINLEKSKQRVTMELEDLQAQYEVSSTKCRQLEKASNNFDKAVEEWKRKVEELQGCLNQSEKENRIYHAELASVKQYNSQLQNDNSKANFEIKRLGEEIKELLSQIGDGGRTFRELQNQKRQMVLERDELQATLQEAESALESEENKLSSSKLENEKLKSDMESRIRDKEDEFEDSKKYFLRLIASLEASFVAEQKTKGELQRLKKRLEADIRELEMALDHSNHTNNELHRNFKRTMVQNSEIQKLLEEEKNAKEEVREKLFVNERRTNSLQNELEKCKTFMEQNDRTQRQLEMEMKDIKGELHSESIRYNSLSQVKRKLEAEILSLRAELVDCNHCIKDSEMKYRQAIIDTMTLSEDLKNMVDRVNKEESLRRGLEVQLKEMVIHLEEVEASSLRGGKKLIEKLEARIKSLEKDLDTEERLRIDAIKDKRKSERKVKDYEVQLEEKERSCHRFNDMILPLHGKIRLLKQQIEEAEEIAALNLAKFKKAIGEAXDSEKRAFIAEQAITKL 1484          

BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000002472 (supercontig:LSalAtl2s:LSalAtl2s147:342440:362242:1 gene:EMLSAG00000002472 transcript:EMLSAT00000002472 description:"snap_masked-LSalAtl2s147-processed-gene-3.1")

HSP 1 Score: 734.176 bits (1894), Expect = 0.000e+0
Identity = 523/1649 (31.72%), Postives = 908/1649 (55.06%), Query Frame = 0

Query:  389 QAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHP--GQPLEAKKDAGGGGR-KKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKD-KDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCT----KFSQYKAEYEE---KIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEE--DVQAL----EDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEES 2020
            QA  ER +H FY L++ A P+ K+K +L +N  DY ++S G  ++   DD E+    + A +I+G +EE++ +++K+ S  M  GNM  +F      +QA +     A K+A + G++   +   F +P++KVG ++VTK QT  Q   ++  IA+ ++E +F++I  + N +L     +   +IG LDIAGFEIFD N FEQ+CIN+ NEKLQQ FN  MF+LEQEEY REGIEW  +DFG+DLQ  ID+ EKPMG++A+++EE  FPKATD+SF  KL  +  S  P F K   + +  A F+V+HYA  V Y+   WL KN DPLN+ +V+  K   +  +I  ++D    G   +A  D   G R +KG  +TVS  YK QL  LM TL  T+P+F+RC++PN  K+ G + + LV+ Q +CNGVL GI ICR+GFPN++ + +F+ RY +L   A+ K   D K A + ++ V++L+   FR+G +K+FFRAG+L ++EE R+ R+ +++   Q+  RG  +R  ++K   Q  A+   QR    Y   + W WW+L+  +KP L+ T    K  Q + E +E   K+   EK++     DR+  + M E     K+ L   L++      +  +   R+     ++++ L ++  RI  E+++   +T+ + ++   Q  +++    +E   A  ++ ++EK +    +K   +++ E++      +RGV++ K   E   DV A     E+KS HLSK++ K E ++ + E+ L  + + + + E+ KRKIE++L   +E + + +    +    + ++E+E A    K+D+E     K  K+ +E++++L E+ E+L  E+G+R KAEK +  L ++LE L + L ++  +TA Q EL   RE+EL  +K  +E+  + HE ++A +R KH+   S+L + ID L  NK   EK KG +E D  +  + L+    AKAE +R  K ++ SI +   KL E  +   ++  +  +L  E  ++   +   EN  +   K   +L TQL +T  L + E R + +L +K ++L  E ++  E++E E E K    K LS   A++   K R E E    +  LE T+ K    I E +  V+ L       +KS+ +L  +L++ SLE +      +  EK+ RNFD+++ E K   D +A E + + ++ R   ++L  L    E+ + +LD   RE + L +E+ DL++  G+  +++HEL+K +R LE    E +  LEEAE   +  E++ +R ++ +  ++ + +R IQ +E+          + +  L + L+ E++ K  A+  KKKL+ D  +LE  ++   K   +  K +K++Q  +++  +  E+ ++ R +  +     ++K  +L  E+ + +  L ++ER +R  + E +E +  +N   S  +    EKR  ++ I A++ E+++    ++   E+AK+A     ++  EL  E+      +  K  LE Q  EL+ +L + E +     +A ++ LE +I  LE +L    +     AK  ++ E+ +KE     E++R++ ++  E  +K+  ++K  K+Q++E EE  +   A+ RK+Q+E E+S
Sbjct:    4 QASDERSFHIFYQLLAGASPEQKKKFIL-ENPKDYTFLSNGIATIPGTDDSEEFNLTNQAMKIMGISEEDSASIWKVISGTMLFGNM--EFRQERNSDQAILPDNTVAQKIAHLLGLNVNDLSKSFLRPRIKVGRDYVTKAQTKEQVEFAIEAIAKAMFERLFKWIVTRINRSLDRTKRQGASFIGILDIAGFEIFDLNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWTFIDFGLDLQPTIDLIEKPMGIMALVDEECWFPKATDKSFVDKLVTSH-SGHPKFVKTDFRGE--ADFSVIHYAGKVDYSAKQWLMKNMDPLNENIVQQLKASQDPFVINIWKDAEIVGMAQQAMSDTQFGARTRKGMFRTVSQLYKEQLGKLMVTLRNTNPNFVRCIIPNHDKRAGKINAWLVLDQLRCNGVLEGIRICRQGFPNRIPFQEFRQRYELLTPNAIPKGFMDGKKACEKMITVLELDRNLFRIGQSKIFFRAGVLAHLEEERDLRITDLVVKFQAYCRGLLARRNYQKRTQQLNAIRILQRNCSAYLKLRNWQWWRLYTKVKPLLQVTKNDEKVIQKETELKEIKDKLESHEKSVQD--LDRQYQQAMEE-----KNILAEQLQAETELCAEAEEMRARLAARKQELEEILHDMEARIEEEEEKALKMTEDKKRL---QLTIQDLEEQLEEEEAARQKMQIEKVQAEAKIK---KYEDELLVFDDLSQRGVKEKKMLDERLSDVTATLAEEEEKSKHLSKLRAKHESTISDLEEKLRKDNQQRQEVERAKRKIETELNDVKEQVMEKKAQVEDFQLQLGKREEELALAMMKMDEEAAAKAKSQKKLREVEAQLTEVVEDLEAEKGARVKAEKQKRELNEELEALKNELLDSLDSTAAQQELRTAREKELAGLKKSLEDEALNHETSVADMRHKHSAEASQLNDVIDQLKKNKSSLEKAKGQLEADNADMTNELKTLSTAKAENERRRKQLENSIAELQMKLQESEKNAADSTDKVSKLIAELENISTALNVAENKASHATKSTGALETQLAETNYLLEEETRQKLALNSKLRSLEHEKDAMTEQLEEEEEAKKSLEKQLSSTLAQLNDVKKRAEEESET-IARLEDTKKKNYKDIEELQHKVDELQAANEKLDKSKKKLAAELDDASLELDVHRGKVLELEKKQRNFDKILTEEKNTTDRIATERDNAERDAREKETKLLNLNRELEDTLARLDDKDREKRLLQNELDDLVNSQGNADKNVHELEKAKRNLEAALVEAKRQLEEAEDEXQAVEDQKMRLEVNMQALKAQYERDIQAREKLARKNDGGMGKHIRDLESELDEERKQKVAAVNAKKKLDADYKDLESTMEINTKLKDDALKQLKKHQAAMKELQRDAEESNQARSDTLQQYKELEKKVKSLEAELIQIQEDLTAAERGRRLAETERDELQEEINSKESRGSLLSDEKRRLDARIAALEEELEEEQGNSEMLMERAKKAQASIEQMTTELAQERGQVQKLENSKMLLERQNKELKAKLQEIETSQRVKAKATIAALESKIANLEEQLSVETAERMGQAKLNRKQEKKLKENLLMLEDERRHADQYKEQNEKVNARLKALKRQLDETEEEMSREKAQKRKTQRELEDS 1632          

BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000008263 (supercontig:LSalAtl2s:LSalAtl2s49:907807:914722:1 gene:EMLSAG00000008263 transcript:EMLSAT00000008263 description:"augustus_masked-LSalAtl2s49-processed-gene-9.4")

HSP 1 Score: 548.125 bits (1411), Expect = 1.578e-173
Identity = 331/708 (46.75%), Postives = 496/708 (70.06%), Query Frame = 0

Query: 1317 LGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRS 2024
            + EQID LN  K KAEKDK +    + E R++++     KA +++  ++      + + K +E   +L + E+ KK++ ME  DL RQIEE +N     +K K +L  QL++ KR+AD E+++R+ LL K++NL  E+++ RE++E E + K + L+ LSK  +++QLW+ R+E EG+ + EELE  + KLQAR+G A  TVE LN+K +S EK +S LQ  +EEMS   +       + EK+ RNFD+VI EWK K D L  E++ +  +CR ++++LF+++  +EE  +QLD V+RENK L++EI+D++DQ+ +GGR+IHE+DK R+RLE+EK E+Q+ALEEAEAALEQEENKVLR QLEL QV+QEI+R+I+EKEEEF+  +K HQ+A+D +  +LEAE R+K EALR+KKKLE DINEL+IAL+ AN++N E Q+ +K+YQ Q++++  G E+E   R + ++ +  A+RK +AL  E+EES+  L   +R +R  + EL ++   +++ +  N      KR  ES +  + A++++M++ +K SE+KAK+AM+DAARLA+ELR EQE +  +++ +RA+E Q+ +++ +L +AE  A K GR  + +LE++IRELE +L   Q R  ++ K  +R ER +KEL FQ EED+KN ERM EL  KLQ KIK+YK+QIEEAEEIAALNLAK+RK Q   EE E+R+
Sbjct:    1 MSEQIDQLNKMKQKAEKDKHSRRLQIDEVRAAMDTINNEKASIEKQNRIATSQYNEVSKKCEEANMSLGDLENSKKKIIMENADLLRQIEEIDNNNNTLSKLKSNLMNQLDEQKRIADDESKERNFLLGKFRNLEHEVDTMREQVEEEGQAKDNALRTLSKTLSDVQLWRQRYEKEGLAKAEELEAAKMKLQARLGXATATVETLNHKAMSLEKEKSHLQCQIEEMSTNADAAAQRCHLMEKKARNFDKVIVEWKNKTDSLQAELDRNQIDCRTFSTDLFKVKTIYEETQQQLDCVRRENKTLSNEIKDIMDQISEGGRNIHEIDKVRKRLEMEKVEMQSALEEAEAALEQEENKVLRLQLELSQVKQEIERRIKEKEEEFDAIKKTHQKALDGMQHALEAENRSKAEALRMKKKLEADINELDIALEHANESNTEAQRTIKKYQNQIKESQLGLENEQIHRDKKRDHLIQAERKCHALQTELEESKTQLEHGDRQRRIAEQELSDTLDQLSDATLQNQSLQTSKRKLESEMQTLHADLEEMISDSKASEDKAKKAMIDAARLAEELRVEQEMAQEKEKDRRAIEFQVKDMQVKLDEAEQLAMKGGRKVVQRLELKIRELESQLDEEQRRLVDNQKNQRRVERRIKELSFQHEEDQKNHERMQELVDKLQNKIKSYKKQIEEAEEIAALNLAKYRKVQMSLEEVEERA 708          

BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000011962 (supercontig:LSalAtl2s:LSalAtl2s84:1272304:1281371:-1 gene:EMLSAG00000011962 transcript:EMLSAT00000011962 description:"maker-LSalAtl2s84-snap-gene-12.11")

HSP 1 Score: 414.846 bits (1065), Expect = 3.400e-118
Identity = 234/653 (35.83%), Postives = 367/653 (56.20%), Query Frame = 0

Query:  270 PHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVS-VESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRD---HPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKM 918
            PHI+ VAE ++  M     NQSI+++GESGAGKT + K  + YFA+VG +    + E  +E +++ ++P++EA GNAKT RNDNSSRFGK+I I FN+   +  ADM  YLLEKSR+ FQAE ER YH FY  M  A  D    CL  ++  D+++++QG    ++ +DD ++     +AF +LG  EE+ + ++++ + ++++GN++ +      + ++   + D  ++  +I     +W++N     K+    E   K      A  +   +A+ IY  +F +I  K N +L   + K  ++IG LDI GFE F  N FEQ CIN+ NEKLQQ FN H+F LEQEEY+REGIEW  +DF  D Q CID+ E  +G+L +L+EE   PK TD+S+  KL++    KC  +Q  +      + F V H+A  V Y    +L+KN+D + +  V + K  SN LL E F +     GQ  +   +   G  KK   KTV S ++  L+ LM  L +T P ++RC+ PN  K     +    + Q +  GVL  + I   G+P++ TY DF  RY +L  +   K    +     ++     + +K+R G +K+FFRAG + YME++R +++      +Q   +G  +R  + ++
Sbjct:  135 PHIYAVAEEAFTRMERDSLNQSIIVSGESGAGKTVSAKYAMRYFATVGGTS---QTETQVEKRVLASSPIMEAIGNAKTTRNDNSSRFGKYIEIDFNKQFHIIAADMRTYLLEKSRVVFQAEDERNYHIFYQ-MCAAREDEFMSCLSLEHPDDFFYLNQGSSPEIDGVDDLKEFMNTREAFHLLGIPEEDQFRIFQILAGILYLGNISVEPSSGRADSESSQITSDERIEEPQI----KKWLVN----RKIITSRESYVKPMNAESALFARDALAKTIYSKLFDWIVVKINMSL-KTSGKTHKFIGVLDIYGFETFAINSFEQFCINYANEKLQQQFNLHVFKLEQEEYLREGIEWKMIDF-YDNQPCIDLIESKLGILDLLDEECRMPKGTDKSWVEKLYD----KCKKWQHFTKNRLSQSAFIVQHFADNVEYESQGFLDKNRDTVMEEQVAVIKASSNTLLCELFSEKSLSSGQKTKITPNP-TGTLKKNSKKTVGSQFRDSLNLLMDALNSTTPHYVRCIKPNDAKAAFQFDPRRGVQQLRACGVLETVRISAAGYPSRWTYYDFFVRYRVLCRSKDVKKNDFRTTCAKIVEKFIGDEDKYRFGKSKLFFRAGQVAYMEKLRSEKLMACGIMIQKHVKGWLARKKYIRL 768          

HSP 2 Score: 167.933 bits (424), Expect = 1.220e-41
Identity = 91/236 (38.56%), Postives = 135/236 (57.20%), Query Frame = 0

Query:   34 YDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTI---LETKDKKVFKKDQVGQV-NPPKFDCSDDMSGLTYLNDACVLWNSVVRY-KNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMR 264
            Y      W+ D  +      +    DG  + V I   LET+   + K+  +  + NP      +D++ L+YL++  VL N  +R+  N  IYTY G+  +AINPY    IY   +I +Y GK   +  PHI+ VAE ++  M     NQSI+++GESGAGKT + K  + YFA+VG +    + E  +E +++ ++P++EA GNAKT RNDNSSRFGK+I I FN+   +  ADMR
Sbjct:    7 YSKGARIWLKDPETVWKAASVTQDYDGKVLHVEITDTLETESINISKESDLPPLRNPDILIGENDLTNLSYLHEPAVLHNLAIRFIDNCAIYTYCGIVLVAINPYSDLQIYGNDTISMYRGKNMGDLDPHIYAVAEEAFTRMERDSLNQSIIVSGESGAGKTVSAKYAMRYFATVGGTS---QTETQVEKRVLASSPIMEAIGNAKTTRNDNSSRFGKYIEIDFNKQFHIIAADMR 239          

BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000006550 (supercontig:LSalAtl2s:LSalAtl2s359:12151:34625:-1 gene:EMLSAG00000006550 transcript:EMLSAT00000006550 description:"maker-LSalAtl2s359-augustus-gene-0.5")

HSP 1 Score: 388.652 bits (997), Expect = 1.109e-110
Identity = 231/675 (34.22%), Postives = 357/675 (52.89%), Query Frame = 0

Query:  267 ECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDP---TMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFE-KPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVK--LEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNY 935
            E PPHIF + +  Y  MM   +NQ I+I+GESGAGKTE+ K ++ Y A++  SGK     + +E +I++ NP+LEA+GNAKTVRNDNSSRFGK+I I F+Q G + GA +  YLLEKSR+  Q + ER YH FY +++    + K K  L D  +  + +  G    +  DD  +      A ++L +++++ +++ K+ + ++HMGN+      +   +  EI    N  +VA I G++ + +I       +    E V      +Q+       A+ IY  +F  I  K NE +F P   T      IG LDI GFE F+ N FEQ CINF NE LQQFF +H+F LEQEEY  E I W +++F +D Q+ +D+   +P+ ++++++EES FPK  DQ+   KLH+   S   N     PK D +  F + H+A  V Y+   +L+KN+D  +  +++L  +  N  L   F           +D   G   +    T+SS +K  L+ LM TL   +P F+RC+ PN  K P   +  L   Q + +G++  I I R G+P   T+ +F  RY  L +     +K   +   A   + +  L    ++LG TKVF +     ++E+ R+  +   +  LQ   +G   R +F K   +K A    Q+  R Y
Sbjct:   96 ELPPHIFAIGDNCYTLMMRTRQNQCIVISGESGAGKTESXKLILQYLAAI--SGK----HSWIEQQILEANPILEAFGNAKTVRNDNSSRFGKYIDIRFSQTGVIEGARIEQYLLEKSRICHQNKDERNYHIFYCMLAGLSKEHKAKLELKDATHYKYLIGGGSTLCDGRDDAAEFADIRSAMKVLMYSDQDIWDILKILAALLHMGNVKYKAKVISNLDATEIPEHVNVERVAAILGVNKQALIAALTSKTIFAQGETVVSTLNTNQSKDVRDAFAKGIYGRLFIHIVKKINEAIFKPELHTSDDRSAIGVLDIFGFENFNTNSFEQFCINFANENLQQFFVRHIFKLEQEEYNLEAINWHHIEF-VDNQEALDLIAIRPLNIMSLIDEESKFPKGNDQTLLNKLHQRHGS---NRNYLKPKSDINTSFGLNHFAGVVFYDTRGFLDKNRDTFSADLLQLIHDSKNKFLQAIF----------ARDLSMGSETRKRAPTLSSQFKKSLESLMNTLGMCNPFFVRCIKPNELKTPMMFDRELCCRQLRYSGMMETIRIRRAGYPIXHTFTEFVDRYRFLISGCPPASKLQDNCRGATAKICQSALGKADYQLGRTKVFLKDAQDLFLEQERDRVLTRKILVLQRCIKGWYYRSLFLK---KKAAAIVIQKYFRAY 747          

HSP 2 Score: 177.178 bits (448), Expect = 1.698e-44
Identity = 85/182 (46.70%), Postives = 122/182 (67.03%), Query Frame = 0

Query:   80 QVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGA 261
            ++  ++P      +DM  L  L++A +L N ++RY + LIYTY+G   +A+NPY+  PIYT   I LY  ++  E PPHIF + +  Y  MM   +NQ I+I+GESGAGKTE+ K ++ Y A++  SGK     + +E +I++ NP+LEA+GNAKTVRNDNSSRFGK+I I F+Q G + GA
Sbjct:   22 RIKAMHPTSVQGVEDMISLGDLHEAGILRNLLIRYNDNLIYTYTGSILVAVNPYQILPIYTAEQIKLYKERKIGELPPHIFAIGDNCYTLMMRTRQNQCIVISGESGAGKTESXKLILQYLAAI--SGK----HSWIEQQILEANPILEAFGNAKTVRNDNSSRFGKYIDIRFSQTGVIEGA 197          

BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000006663 (supercontig:LSalAtl2s:LSalAtl2s36:1281657:1296608:-1 gene:EMLSAG00000006663 transcript:EMLSAT00000006663 description:"maker-LSalAtl2s36-snap-gene-13.49")

HSP 1 Score: 325.865 bits (834), Expect = 1.849e-91
Identity = 209/653 (32.01%), Postives = 333/653 (51.00%), Query Frame = 0

Query:  269 PPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFA-SVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQA-ELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQG-------------KVSVE----------SIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIK--SEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQT------CSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFE-KPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASP-KPDPHAH--------FAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVF 878
            PPHIF +A+ +++ M  + K+QS++++GESGAGKTE+TK ++ Y   + G+ G+       LE+KI+  NP+LEA+GNAKT RN+NSSRFGKFI I F+   K+ G  +  YLLE++R+T Q+ E ER YH FY L + A  DLK K  L  ++  + ++  G             + S E          ++DD +D          LG +E +  +VY   + V+H+GN++ +  P        +   SE++    AE+  +D++ +        ++       KG          +AS++   +A+ IY  +F +I  + N ++  P  K   YIG LDIAGFE F  N FEQ CIN+CNEKLQQFFNQ +   EQ  Y +EG+    + F +D Q CID+ E K  G+ ++L+EES  PK   Q F   +H        +F+ + P K     H        F + H+A  V Y   S++EKN D L+ ++  L      N  I+           + +   G  + K    +V + +K QL++LM  L +T  +FIRC+ PN    P   + G ++ Q QC G+   + + ++G+P++ ++ +    Y+      + +    +   K +   + L  E F+ G TK+ 
Sbjct:  168 PPHIFAIADKAFRDMKVLKKSQSVVVSGESGAGKTESTKYILKYLCDNFGSKGR------GLEEKILNANPILEAFGNAKTTRNNNSSRFGKFIEIHFDTSCKVVGGYISHYLLERARVTSQSSEEERNYHIFYQLCAGAPSDLKNKLHLG-SVEKFRYLKHGCTRYFGSSSIPSDRCSAEFKRLGPLKDPNLDDVKDFLNVDKDLSNLGMSELDRLDVYTAIASVLHIGNISFEDDPDDNRGGCRVTEDSENSLNITAELLKLDTDELRRALTARVMQATKGGGYKGTVIMVPLKVHEASNARDALAKAIYSRLFDYIVKRINNSI--PFEKSAYYIGVLDIAGFEYFTVNSFEQFCINYCNEKLQQFFNQRILKEEQMLYEKEGLGVKKISF-VDNQDCIDLIESKGNGVFSLLDEESKLPKPNHQHFTNAVHAQ---NTGHFRLSLPRKSKLRGHREIRDDDGFLIRHFAGAVCYQTASFIEKNNDALHASLEALAXEEXQNPFIQSL-------FASARSTSGSMKGKLTFLSVGNKFKSQLEELMDKLRSTGTNFIRCIKPNGKMIPQLFQGGSILSQLQCAGMAXVLELMQQGYPSRTSFSELYKLYSGYLPPELTRLDP-RHFCKVLFKALGLNDEDFKFGLTKML 799          

HSP 2 Score: 163.696 bits (413), Expect = 1.778e-40
Identity = 91/230 (39.57%), Postives = 134/230 (58.26%), Query Frame = 0

Query:   41 WVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVG----QVNPPKFDCS---DDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFP-IYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFA-SVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGA 261
            WV +   G     ++D TD       I +  D+  +    +     ++ P + D +   DD  GL YLN+A +L N  VRY  + IYTY     IAINPYK    +Y+ +++  Y GK     PPHIF +A+ +++ M  + K+QS++++GESGAGKTE+TK ++ Y   + G+ G+       LE+KI+  NP+LEA+GNAKT RN+NSSRFGKFI I F+   K+ G 
Sbjct:   45 WVSEPEQGFILGRIVDITDDGVSIQPINDNNDEFNYNSKAISASFDRLYPSEDDEASDVDDNCGLMYLNEATLLHNVRVRYGLDKIYTYVANILIAINPYKEIKNLYSSKTVGEYRGKSLGTMPPHIFAIADKAFRDMKVLKKSQSVVVSGESGAGKTESTKYILKYLCDNFGSKGR------GLEEKILNANPILEAFGNAKTTRNNNSSRFGKFIEIHFDTSCKVVGG 268          

BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000006256 (supercontig:LSalAtl2s:LSalAtl2s340:648475:653143:1 gene:EMLSAG00000006256 transcript:EMLSAT00000006256 description:"maker-LSalAtl2s340-augustus-gene-6.20")

HSP 1 Score: 297.745 bits (761), Expect = 9.176e-82
Identity = 200/692 (28.90%), Postives = 346/692 (50.00%), Query Frame = 0

Query:  270 PHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAEL-ERC-YHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQ---AEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDP--TMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLL-------------IECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRV-------GEVLSWLQSQARGKASRMVFKKMQDQKLA-LYSCQRTIR 933
            PH++ +A  SY  M++  KNQ  +I+GESGAGKT +   V+     +G +  R     +LEDKI+Q NP+LEA+GNAKT  N NSSRF K + I ++  GK++GA + ++LLE SR+T   ++ E C +H FY L+       K      + I+ +     G      I D ++      A + LGF E +   +Y + + ++++G++  DF PV  ++     ++ + D   K+ ++ G++ E +++ F    +    E +T+  +  +A       A+ +Y   F +I    N+ L        +   IG LDI GFE  + N FEQ+CIN  NE+L  +FNQ +F +E+EEY  EGI      +  +      +  +P+GL A+++EE  FP +T+ +  +KL+ NL      F   S        F + HYA  V Y+   +L+KN++ L   ++ + +   N ++             + C  +      +  K A     +    +T+S+F++  L DL+K      P FI+C  PN  +KPG +++  +M Q Q +GV   +   + G+P ++T+ +F  RY  L  +   +    K+  + ++  ++L  +   LG TKVF +   + Y+ ++ E ++       G V SWL   A+    +M +  ++D  LA  +SC+   R
Sbjct:  407 PHVYAMANSSYHNMIHEKKNQRFIISGESGAGKTMSANWVMKMLVYLGKAPNR-----NLEDKILQINPILEAFGNAKTPLNGNSSRFAKVVEITYSVNGKVTGARISVFLLEHSRVTSDRDIREDCNFHIFYYLVKGLNHYGKSNDYYLNEIHRFL----GDQVTPLIKDYDNFIV---ALKSLGFRENDLETIYTIIASILNIGDL--DFTPVETDDNVGGCKVSNPDILDKIVKLLGVNKEELVDCFQNSTVSTKGEVITRSNSPEEAKFMRDAFAKGLYSRFFDYIVYSINKLLSYSLNVYGESNSIGILDIFGFETLETNSFEQLCINTTNEQLFYYFNQVVFRMEKEEYEMEGIFVKMESYSSNHSILDLLLSRPLGLFALIDEECKFPSSTETTLLSKLNNNL----EKFDAYSHPKQEFQLFVIQHYAQKVEYSPNQFLDKNRNFLPPELIAVMRYSQNEIIRFLFNCPITKTGRLSCSNNSAPVTPDICKMATETHSQTRSQQTLSTFFRYSLTDLLKNTLNGTPHFIKCFKPNKFQKPGNLDTSYLMSQLQYSGVFQTVKXRQIGYPCRLTFAEFLRRYCFLGFSFDERVVATKENCQILM--LRLRMDGXALGKTKVFLKYYHVEYLSKLYEYQIRKIIKVQGIVRSWL---AKINTQKMKWAVLRDLMLAKRFSCRWRYR 1075          

HSP 2 Score: 127.487 bits (319), Expect = 2.708e-29
Identity = 70/177 (39.55%), Postives = 98/177 (55.37%), Query Frame = 0

Query:  112 VRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMR---------------RSECPPHIF 273
            +RY    IYTY G   I+INP K   IY+   +  Y  K + +  PH++ +A  SY  M++  KNQ  +I+GESGAGKT +   V+     +G +  R     +LEDKI+Q NP+LEA+GNAKT  N NSSRF K + I ++  GK++GA +                R +C  HIF
Sbjct:  362 MRYNQNKIYTYIGEIIISINPCKTLNIYSAHEMCKYRNKSKYDNLPHVYAMANSSYHNMIHEKKNQRFIISGESGAGKTMSANWVMKMLVYLGKAPNR-----NLEDKILQINPILEAFGNAKTPLNGNSSRFAKVVEITYSVNGKVTGARISVFLLEHSRVTSDRDIREDCNFHIF 533          

BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000004494 (supercontig:LSalAtl2s:LSalAtl2s234:519519:622059:-1 gene:EMLSAG00000004494 transcript:EMLSAT00000004494 description:"maker-LSalAtl2s234-augustus-gene-5.11")

HSP 1 Score: 292.352 bits (747), Expect = 1.851e-79
Identity = 235/871 (26.98%), Postives = 402/871 (46.15%), Query Frame = 0

Query:   82 GQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPP----HIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPD--LKQKCLLSD-NIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAV--KVAEICGIDSEWMINYFCKPKLKV---GTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPM-GLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIEC--FRD---HPGQPLEAKKDAGGGGRKKGGGK---TVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVF-------KKMQDQKLA 924
            GQ+     D   DM+ ++ +++  +  N  VRY+N+ IYTY+G   +A+NPYK  PIY  + + +++    S          +G+  G     MN G  + ++    S   +      +I++      S        SL+            W     + +   +R    +   +++ G L          PPH+F +A+ +Y+ +     +QS++I+GESGAG                                           NAKT+RNDNSSRFG+F+++ F+   K+ G  +  YLLE+SR+T Q+E ER YH FY L S A  D  L  +  +   + Y+Y  VS G + +E +DD         AF ++   +E+   ++  T                   E+A+I   D  +  KVA + G+  E +I      ++ +    TE   K Q   +A  +   +++ +Y   F ++ +  N+   +P     Q+IG LDI GFE F+ N FEQ+CIN+ NEKL +FFN ++F LEQE Y  E I+++++ F  D   C+++ EKP   +L +L E+   P   D ++   +        P+F K   +      F + HYA TV+Y++  +++KN+D   D   +         + E   F+D      Q  E+   A G  ++    K   TVS  ++ QL  L++ L +T+P ++RC+ PN  K     +S LV+ Q +  G+L  I I ++GFP  M +++F +RY  L         + + A KA++ +  L + +++LG  K+F R  +   +EE R   +      +Q   +G+  R+++       KK+Q++ L 
Sbjct:    8 GQIGCRPQDGVPDMTVISNIDEHGINENLQVRYENDNIYTYTGTILVAVNPYKELPIYDSKFVSIFLLFHYSSLAGLKTLMFYGIYIGGCCQEMNHGGAEDLVAVLSSTLYRC---LYIIAFATPPSLSICLNISAISLKSH----------WFVEMLLPHTRCNRILFSLEPVYSKIGAL----------PPHVFAIAQAAYKNLQADCVDQSLVISGESGAG-------------------------------------------NAKTIRNDNSSRFGRFMQVCFDSKYKIKGCVIQDYLLEQSRITTQSEQERNYHIFYQLTSAAYRDSELATRFHIEPASKYNYLNVS-GCIRLEGVDDAAKFDALRLAFGVVQIPKEKIDGIFSDTD-----------------NEKAKITKADGIILEKVAYLLGMLKEDLIQVSLLRQINIRGNVTEIPLKYQ---EARENRHAMSKALYSRTFAWLVNHINKCT-NPGKDMAQFIGILDIFGFENFNTNSFEQLCINYTNEKLHKFFNHYVFALEQEAYRVEEIQFSHITF-TDNTHCVELIEKPPKCILKLLAEQCHMPGGCDAAYLTNIRSE-FDYHPDFVKGDDRRKWDKEFGIRHYAGTVTYSVAGFVDKNRDSQQDVFFDFLAKSKCEFVKELVEFKDLLSKVAQLGESVSKAEGTVKRVMTNKAKPTVSDAFRLQLQVLVEVLQSTNPWYVRCIKPNMEKASNHYDSKLVLDQLKYLGMLEIIRIRKQGFPIHMPFEEFISRYKCLHRKKKLPLDQ-RHACKALIQLEGLPSMEWQLGKNKIFLRCCVHEPLEEKRNWVLNTRAIMIQKTWKGQKQRLIYIEIRNAAKKIQERYLT 787          

BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000007267 (supercontig:LSalAtl2s:LSalAtl2s408:622020:640607:-1 gene:EMLSAG00000007267 transcript:EMLSAT00000007267 description:"maker-LSalAtl2s408-augustus-gene-5.36")

HSP 1 Score: 276.944 bits (707), Expect = 1.691e-76
Identity = 199/629 (31.64%), Postives = 321/629 (51.03%), Query Frame = 0

Query:  267 ECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQ----GGKLSGADMVIYLLEKSRLTFQAELERCYHAFY---NLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKV-AEICGIDSEWMINYFCKPKLKVGTEWVTKGQ----TCS--QASSSVAGIARKIYELVFRFICDKCNETL-FDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKC--IDMFEKPMGLLAILEE----ESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ----PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKD-KDAAKAVLNVVKLEAEK 869
            E PPH++ +A   Y+ M    ++Q ++I+GESGAGKT   K +++Y + V   G   E  + ++  I+Q+NP+LEA+GNAKT+RN+NSSRFGK++ I F      GG++S      +LLEKSR+  Q   ER +H FY   N +     D+K +  +++  +  +        V+  +D++  +   DA   +G +E E  +V  L S ++H+GN+       G  + A I ++ N +   A +  I S  +        ++  T+W +K +    TC+  Q+  +   +A+ +Y  +F ++  + N  +      K +  +G LDI GFEIF  NGFEQ CIN+ NEKLQQ F +     EQEEYV+EGIEW  + +  +   C  I+  +   G+L IL +    +    + +D +F +KL ++  S   +FQ      D    F + HYA  V+YN+  + E+NKD  ++ ++E+ + GSNN  I        Q    P+ A            G K +S     Q + L+ +L + +PS+IRC+ PN  KKP   E+  VMHQ +  G+   + + R GF  +  +D F  RY IL          D +     +LN  +L  +K
Sbjct:   77 ENPPHVYALAXNMYRNMTIDNEHQCVIISGESGAGKTVAAKYIMNYLSQVSGGG---ESASHIKSVILQSNPLLEAFGNAKTIRNNNSSRFGKYVEILFEHSRPVGGQISN-----FLLEKSRVVRQNPKERNFHIFYQFVNGLEGESDDMKSRFGVAELEFYNYLNEHACYHVDDTNDQKGFEETMDAMTTMGMSESEKNDVLTLVSGILHVGNV---IFSEGVNDTA-IPADINLLDYPAYLLQIXSSTLATKLTSRVME--TKWGSKTEIVNVTCNVMQSEYTRDALAKGLYSRLFDYLVQRANGAMKVQSKNKDLLNLGILDIYGFEIFGKNGFEQFCINYVNEKLQQIFIELTLKAEQEEYVQEGIEWKEISYFNNAIVCELIESKKPSPGVLPILNDICSTQHGVKEGSDMNFKSKLRDH-CSMHKHFQ------DCAQGFIIHHYAGVVTYNVDGFCERNKDLFHNDLIEMMQ-GSNNQFIRSLFPETTQSKKRPITA------------GTKIIS-----QANKLVSSLMSCNPSYIRCIKPNETKKPRDWENARVMHQIEYLGLKENVRVTRAGFVYRRPFDKFLYRYAILTKETWPHYHGDSRKGVVHILNAKQLLKQK 666          

HSP 2 Score: 145.591 bits (366), Expect = 5.061e-35
Identity = 69/178 (38.76%), Postives = 114/178 (64.04%), Query Frame = 0

Query:   93 DDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQ----GGKLSGADMRRS 266
            DDM+ L+ + ++ ++ N   RY ++ I+TY G   I++NP+K    +TQ+ +D+Y G    E PPH++ +A   Y+ M    ++Q ++I+GESGAGKT   K +++Y + V   G   E  + ++  I+Q+NP+LEA+GNAKT+RN+NSSRFGK++ I F      GG++S   + +S
Sbjct:   16 DDMTLLSSVTESAIMENMKKRYLDDWIFTYIGQVLISVNPFKHMSYFTQKEVDMYQGAALYENPPHVYALAXNMYRNMTIDNEHQCVIISGESGAGKTVAAKYIMNYLSQVSGGG---ESASHIKSVILQSNPLLEAFGNAKTIRNNNSSRFGKYVEILFEHSRPVGGQISNFLLEKS 190          

BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|110825729|sp|P05661.4|MYSA_DROME (RecName: Full=Myosin heavy chain, muscle)

HSP 1 Score: 1805.8 bits (4676), Expect = 0.000e+0
Identity = 973/1779 (54.69%), Postives = 1297/1779 (72.91%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEG---EASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPH--AHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGK-------TVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
            RR+E PPHIF +++G+Y  M+    NQS+LITGESGAGKTENTKKVI+YFA+VGAS K  E    + SLED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+  YLLEK+R+  Q  LER YH FY +MS +VP +K  CLL+DNIYDY  VSQGKV+V SIDD E+      AF ILGF ++E  +VY++T+ VMHMG M   F   G+EEQAE   E+   +V+++ G D+  +     KP++KVG E+VT+G+   Q ++S+  + + +++ +F+++  KCNETL D   K+  +IG LDIAGFEIF+YNGFEQ+CINF NEKLQQFFN  MFV+EQEEY +EGI W  +DFGMDL  CID+ EKPMG+L+ILEEES+FPKATDQ+F+ KL    L K   FQK  P       AHFA+ HYA  VSYN+T WLEKNKDPLNDTVV+ FK   N LLIE F DH GQ       +GGG + KGG         TVSS YK QL+ LM TL +T P F+RC++PN  K+PG V++ LVMHQ  CNGVL GI ICRKGFPN+M Y DFK RY IL    +      K A+K ++   +L  + +RLGHTKVFFRAG+LG MEE R++R+G+++SW+Q+ ARG  SR  FKK+Q+Q++AL   QR +R Y   +TW W++LW  +KP L  ++     A  EEK   AE+     +  RK++E ++  LL +K+ L  +L     A+QD  ++N ++     D++ QL ++  R+  E+  R+ L QQ+ K   + S L+++I  +E  +  AEQDK  KD QIR L +E+ HQ E+I KL +EK+   +  QKT E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKKV+ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE  +  K+ +Q KELQ+R+EEL+EE+  ER +RAKAEK R+ L ++LE+LG RLEEAG  T+ Q+ELNKKRE EL +++ ++EE NI HE TLA LR KHN+ ++E+ EQ+D LN  K KAE D+     +L + R++ +   R KA  ++  K +Q ++ +  SKLDE  R LN+ ++ KK+L +E  DL RQ+EE E+ ++Q +K KISLTTQLEDTKRLAD E+R+R++LL K++NL  +L++ RE++E E E K+D  + LSKA AE Q+W+S++E++G+ R EELE  + KLQAR+ EAEET+E+LN K +  EK++ RL  ++E++ LE +R +A A  +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE  EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLR+QLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+D     E+E R R + +E +GI++R+ NAL  E+EESR LL  ++R +RQ + EL ++   +NE+S+ N      KR  ES +  + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALE Q+ EL+ RL +AEANA K G+ A+ KLE R+RELE EL   Q R +++ K  +++ER VKEL FQ EEDRKN ERM +L  KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+  AE++
Sbjct:  147 RRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMK--FKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETL-DTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQ-------SGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPRGIKDLDCPKKASKVLIESTELNEDLYRLGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1915          

HSP 2 Score: 330.487 bits (846), Expect = 7.668e-90
Identity = 156/266 (58.65%), Postives = 197/266 (74.06%), Query Frame = 0

Query:    1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEG---EASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            MP  +  ++  D DP+P+L VS E +   +SKPYD KKSCW+PD+  G Y  G I +T GD V+V  L+  + +  K ++V +VNPPKF+  +DM+ +T LN  CVL N   RY  +LIYTYSGLFC+AINPYKR+P+YT R   +Y GKRR+E PPHIF +++G+Y  M+    NQS+LITGESGAGKTENTKKVI+YFA+VGAS K  E    + SLED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+
Sbjct:    1 MPKPV--ANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEG-YLLGEIKATKGDIVSVG-LQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 262          

BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|127773|sp|P24733.1|MYS_ARGIR (RecName: Full=Myosin heavy chain, striated muscle)

HSP 1 Score: 1595.1 bits (4129), Expect = 0.000e+0
Identity = 848/1777 (47.72%), Postives = 1229/1777 (69.16%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEAS------LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPH---AHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKD-KDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
            R++E PPH+F VA+ +YQ M+   +NQS LITGESGAGKTENTKKVI Y A V  + K+K+ EAS      LED+I+Q NPVLEA+GNAKT RN+NSSRFGKFIRI F   GK++GAD+  YLLEKSR+T+Q   ER YH FY + S+A+P+L    L++ +   Y +++QG ++V++IDD E+ +   +AF ILGF +EE  +++K T+ ++HMG M   F    +EEQAE      A KVA +CGI++  ++    KPK+KVGTE VTKGQ  +Q  +SV  +A+ +Y+ +F ++  + N+TL D   K+  YIG LDIAGFEIFD+N FEQ+CIN+ NE+LQQFFN HMF+LEQEEY +EGI W  +DFGMDLQ CID+ EKPMG+L+ILEEE +FPKA D+SF  KL++N + K   F K      P+   AHF + HYA  V Y++T WLEKNKDP+N+ VV L       L+ E F+     P E          K    +T+S+ ++  L+ LMK LY+T P F+RC++PN  K+PG V++ LV+HQ QCNGVL GI ICRKGFP+++ Y +FK RY+ILA  A+ +   D K  ++ +L  ++++  ++RLG TKVFF+AG+LG +EE+R++R+ +++S  Q+  RG   R  +KK+QDQ++ L   QR IR + + + W WW+L+  +KP L   +  +   E  +++   ++++ K    +K++E  +  LL QK++L L L++   ++ D  ++  ++    AD + Q+ E+  R+  E+     L   + K+++  + L+++I  +E  L  AEQDK  KD QI TL+ E+  Q E I KL +EK+ +++  +KT + +QA EDK +HL+K+K KLEQ+LDE ED LE EKKV+ D EK KRK+E DLK TQE + DLERVK EL + V+RKE E +++N+K++DE  L  +  ++ KELQ+R+EEL+EEL  ER +RAK EK R+ L ++LE+LG RL+EAG  T+ Q+ELNKKRE EL +I+ ++EE ++ HE  ++ALR KH +  +E+ +Q+D L   K K EKDK +++R++ +  S +   ++ K   ++  K  +  + D N++L++  R++NE +SQK RL+ E  DL RQ+E+ E+ ++  +K+K  L++QLED +R  + E R RS L  + +N+  ++++ RE++E E E KSD  + LSKA  EIQ W+S+FE+EG  R EELE  + KL  ++ EAE+T EA N K  + EK++SRLQ +LE+MS+E +R +A+    EK+ R FD+   EW+AK + L  E+E S KE R Y++EL+R++A+ EE  + +  ++RENKNLADEI DL DQL +GGRS HELDK RRRLE+EKEELQAALEEAE ALEQEE KV+R+QLE+  VR EID++IQEKEEEF+NTR+NHQRA++S+ ASLEAE + K +A+RIKKKLE DINELE+ALD +N+  AE +K VKRYQ Q+R+     E+E R R E +E+  +A+R+   ++GEVEE RA L  +ER+++  D EL ++   VNE++S  +    +KR  E  I+A+Q ++D+M  + K ++E+ K+AM DAARLADELR+EQ+HS   ++ ++ LESQ+ E + RL +AEA++ K G+  + KLE R+ ELE EL + Q R +E+ K  ++A+R +KEL FQ +EDRKNQER+ EL  KL  KIKT+K+Q+EEAEEIAA+NLAK+RK+Q E EE+E+R+ TA+ +
Sbjct:  144 RKTEIPPHLFSVADNAYQNMVTDRENQSCLITGESGAGKTENTKKVIMYLAKVACAVKKKDEEASDKKEGSLEDQIIQANPVLEAYGNAKTTRNNNSSRFGKFIRIHFGPTGKIAGADIETYLLEKSRVTYQQSAERNYHIFYQICSNAIPELNDVMLVTPDSGLYSFINQGCLTVDNIDDVEEFKLCDEAFDILGFTKEEKQSMFKCTASILHMGEMK--FKQRPREEQAESDGTAEAEKVAFLCGINAGDLLKALLKPKVKVGTEMVTKGQNMNQVVNSVGALAKSLYDRMFNWLVRRVNKTL-DTKAKRNYYIGVLDIAGFEIFDFNSFEQLCINYTNERLQQFFNHHMFILEQEEYKKEGIAWEFIDFGMDLQMCIDLIEKPMGILSILEEECMFPKADDKSFQDKLYQNHMGKNRMFTKPGKPTRPNQGPAHFELHHYAGNVPYSITGWLEKNKDPINENVVALLGASKEPLVAELFK----APEEPAGGGKKKKGKSSAFQTISAVHRESLNKLMKNLYSTHPHFVRCIIPNELKQPGLVDAELVLHQLQCNGVLEGIRICRKGFPSRLIYSEFKQRYSILAPNAIPQGFVDGKTVSEKILAGLQMDPAEYRLGTTKVFFKAGVLGNLEEMRDERLSKIISMFQAHIRGYLIRKAYKKLQDQRIGLSVIQRNIRKWLVLRNWQWWKLYSKVKPLLSIARQEEEMKEQLKQMDKMKEDLAKTERIKKELEEQNVTLLEQKNDLFLQLQTLEDSMGDQEERVEKLIMQKADFESQIKELEERLLDEEDAAADLEGIKKKMEADNANLKKDIGDLENTLQKAEQDKAHKDNQISTLQGEISQQDEHIGKLNKEKKALEEANKKTSDSLQAEEDKCNHLNKLKAKLEQALDELEDNLEREKKVRGDVEKAKRKVEQDLKSTQENVEDLERVKRELEENVRRKEAEISSLNSKLEDEQNLVSQLQRKIKELQARIEELEEELEAERNARAKVEKQRAELNRELEELGERLDEAGGATSAQIELNKKREAELLKIRRDLEEASLQHEAQISALRKKHQDAANEMADQVDQLQKVKSKLEKDKKDLKREMDDLESQMTHNMKNKGCSEKVMKQFESQMSDLNARLEDSQRSINELQSQKSRLQAENSDLTRQLEDAEHRVSVLSKEKSQLSSQLEDARRSLEEETRARSKLQNEVRNMHADMDAIREQLEEEQESKSDVQRQLSKANNEIQQWRSKFESEGANRTEELEDQKRKLLGKLSEAEQTTEAANAKCSALEKAKSRLQQELEDMSIEVDRANASVNQMEKKQRAFDKTTAEWQAKVNSLQSELENSQKESRGYSAELYRIKASIEEYQDSIGALRRENKNLADEIHDLTDQLSEGGRSTHELDKARRRLEMEKEELQAALEEAEGALEQEEAKVMRAQLEIATVRNEIDKRIQEKEEEFDNTRRNHQRALESMQASLEAEAKGKADAMRIKKKLEQDINELEVALDASNRGKAEMEKTVKRYQQQIREMQTSIEEEQRQRDEARESYNMAERRCTLMSGEVEELRAALEQAERARKASDNELADANDRVNELTSQVSSVQGQKRKLEGDINAMQTDLDEMHGELKGADERCKKAMADAARLADELRAEQDHSNQVEKVRKNLESQVKEFQIRLDEAEASSLKGGKKMIQKLESRVHELEAELDNEQRRHAETQKNMRKADRRLKELAFQADEDRKNQERLQELIDKLNAKIKTFKRQVEEAEEIAAINLAKYRKAQHELEEAEERADTADST 1913          

HSP 2 Score: 302.753 bits (774), Expect = 2.750e-81
Identity = 154/312 (49.36%), Postives = 200/312 (64.10%), Query Frame = 0

Query:    9 DGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA------SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM-------------RRSECPPHIFGVAEGSYQGMMNVGK--NQSILITGESG 299
            D  DPD   +L V  +   K ++  +D KK+CWVPD+  G +    I S+ GD++TV I+     +  KKD +  +NPPKF+  +DM+ +TYLN+A VL+N   RY + LIYTYSGLFCIA+NPY+R PIYT   I  Y GKR++E PPH+F VA+ +YQ M+   +NQS LITGESGAGKTENTKKVI Y A V  + K+K+ EA      SLED+I+Q NPVLEA+GNAKT RN+NSSRFGKFIRI F   GK++GAD+             + +E   HIF      YQ   N     N  +L+T +SG
Sbjct:    4 DFSDPD-FQYLAVDRKKLMKEQTAAFDGKKNCWVPDEKEG-FASAEIQSSKGDEITVKIVADSSTRTVKKDDIQSMNPPKFEKLEDMANMTYLNEASVLYNLRSRYTSGLIYTYSGLFCIAVNPYRRLPIYTDSVIAKYRGKRKTEIPPHLFSVADNAYQNMVTDRENQSCLITGESGAGKTENTKKVIMYLAKVACAVKKKDEEASDKKEGSLEDQIIQANPVLEAYGNAKTTRNNNSSRFGKFIRIHFGPTGKIAGADIETYLLEKSRVTYQQSAERNYHIF------YQICSNAIPELNDVMLVTPDSG 307          

BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|125987844|sp|P79293.2|MYH7_PIG (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta)

HSP 1 Score: 1510.35 bits (3909), Expect = 0.000e+0
Identity = 807/1776 (45.44%), Postives = 1204/1776 (67.79%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPG--QPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            +RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+  YLLEKSR+ FQ + ER YH FY ++S+  P+L    L+++N YDY ++SQG+ +V SIDD E++    +AF +LGF  EE  ++YKLT  +MH GNM   F    +EEQAE    + A K A + G++S  ++   C P++KVG E+VTKGQ   Q   +   +A+ +YE +F ++  + N TL   T +  QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW  +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K  NFQK  + K  P AHFA++HYA TV YN+  WL+KNKDPLN+TVV+L+K  S  LL   F ++ G   P+E  K       K    +TVS+ ++  L+ LM  L +T P F+RC++PN  K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL  AA+ + +    +  A+ +L  + ++  +++ GHTKVFF+AG+LG +EE+R++R+  +++ +Q+Q+RG  SRM FKK+ +++ +L   Q  IR +   K W W +L+  IKP LK  +  +  A  +E+    ++ + K+ A RK++E     LL +K++L L +++    + D  ++  ++      ++ ++ E+  R+  E++    LT ++ K++ + S+L+ +I+ +E  LA  E++K   + +++ L EE+    E+I KL +EK+ +Q+  Q+  +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE  K +L + +++K+ E  A+NA+I+DE  LG +  K+ KELQ+R+EEL+EEL  ER +RAK EK RS L ++LE++  RLEEAG  T+ Q+E+NKKRE E  +++ ++EE  + HE T AALR KH ++++ELGEQIDNL   K K EK+K   + +L +  S++E  ++AKA L++  + ++  + +  SK +E  R++N+  SQ+ +L+ E  +L RQ++E E  ++Q  + K++ T QLED KR  + E + +++L    ++   + +  RE+ E E E K++  + LSKA +E+  W++++ET+ + R EELE  + KL  R+ +AEE VEA+N K  S EK++ RLQ ++E++ ++ ER++AAA   +K+ RNFD+++ EWK K ++   E+E+S KE R+ ++ELF+L+ A+EE++E L+  KRENKNL +EI DL +QLG  G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE  Q++ E++RK+ EK+EE    ++NH R +DSL  SL+AE R++ EALR+KKK+E D+NE+EI L  AN+  AE QK VK  Q  L+DT    +D  R   +++E + I +R+ N L  E+EE RA++  +ERS++  + EL E+   V  + S NT  +++K+  E+ +  +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S   +R K+ +E  + +L+ RL +AE  A K G+  + KLE R+RELE EL + Q R +ES K  +++ER +KEL +Q EEDRKN  R+ +L  KLQ K+K YK+Q EEAEE A  NL+KFRK Q E +E+E+R+  AE
Sbjct:  146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVMYATGALAKAVYEKMFNWMVTRINTTL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGRPEAHFALIHYAGTVDYNIIGWLQKNKDPLNETVVDLYKKSSLKLLSNLFANYAGADTPVEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMSVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEMERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMEADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914          

HSP 2 Score: 276.174 bits (705), Expect = 3.474e-73
Identity = 132/255 (51.76%), Postives = 175/255 (68.63%), Query Frame = 0

Query:   14 DPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            + +P+L  S + + + +++P+D KK  +VPD     + +  I S +G KVT      K   V K+DQV Q NPPKFD  +DM+ LT+L++  VL+N   RY + +IYTYSGLFC+ INPYK  P+Y    +  Y GK+RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+
Sbjct:   11 EAAPYLRKSEKERLEAQTRPFDLKKDVYVPDDKEE-FVKAKILSREGGKVTAETEHGKTVTV-KEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTINPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263          

BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|81871557|sp|Q91Z83.1|MYH7_MOUSE (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta)

HSP 1 Score: 1508.43 bits (3904), Expect = 0.000e+0
Identity = 807/1774 (45.49%), Postives = 1202/1774 (67.76%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            +RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+  YLLEKSR+ FQ + ER YH FY ++S+  P+L    L+++N YDY ++SQG+ +V SIDD E++     AF +LGF  EE  ++YKLT  +MH GNM   F    +EEQAE    + A K A + G++S  ++   C P++KVG E+VTKGQ   Q S ++  +A+ +YE +F ++  + N TL   T +  QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW  +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K  NFQK  + K    AHF++VHYA TV YN+  WL+KNKDPLN+TVV L++  S  LL   F ++ G    A K   G  +K    +TVS+ ++  L+ LM  L +T P F+RC++PN  K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL  AA+ + +    +  A+ +L  + ++  +++ GHTKVFF+AG+LG +EE+R++R+  +++ +Q+Q+RG  SRM FKK+ +++ +L   Q  IR +   K W W +L+  IKP LK  +  +  A  +E+    +  + K+ A RK++E     LL +K++L L +++    + D  ++  ++      ++ ++ E+  R+  E++    LT ++ K++ + S+L+ +I+ +E  LA  E++K   + +++ L EE+    E+I KL +EK+ +Q+  Q+  +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE  K +L + +++K+ E  A+NA+I+DE  LG +  K+ KELQ+R+EEL+EEL  ER +RAK EK RS L ++LE++  RLEEAG  T+ Q+E+NKKRE E  +++ ++EE  + HE T AALR KH ++++ELGEQIDNL   K K EK+K   + +L +  S++E  ++AKA L++  + ++  + +  SK +E  R++N+  SQ+ +L+ E  +L RQ++E E  ++Q  + K++ T QLED KR  + E + +++L    ++   + +  RE+ E E E K++  + LSKA +E+  W++++ET+ + R EELE  + KL  R+ +AEE VEA+N K  S EK++ RLQ ++E++ ++ ER++AAA   +K+ RNFD+++ EWK K ++   E+E+S KE R+ ++ELF+L+ A+EE++E L+  KRENKNL +EI DL +QLG  G+SIHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE  Q++ EI+RK+ EK+EE    ++NH R +DSL  SL+AE R++ EALR+KKK+E D+NE+EI L  AN+  AE QK VK  Q  L+DT    +D  R   +++E + I +R+ N L  E+EE RA++  +ERS++  + EL E+   V  + S NT  +++K+  ++ +  +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S   +R K+ +E  + +L+ RL +AE  A K G+  + KLE R+RELE EL + Q R +ES K  +++ER +KEL +Q EEDRKN  R+ +L  KLQ K+K YK+Q EEAEE A  NL+KFRK Q E +E+E+R+  AE
Sbjct:  146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDSAFDVLGFTPEEKNSIYKLTGAIMHFGNMK--FKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVSYAIGALAKSVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNILGWLQKNKDPLNETVVGLYQKSSLKLLSNLFANYAGADAPADK-GKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIVKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSTGKSIHELEKIRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914          

HSP 2 Score: 275.018 bits (702), Expect = 7.787e-73
Identity = 132/254 (51.97%), Postives = 176/254 (69.29%), Query Frame = 0

Query:   17 PWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVF--KKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            P+L  S + + + +++P+D KK  +VPD     + +  I S +G KVT    ET++ K    K+DQV Q NPPKFD  +DM+ LT+L++  VL+N   RY + +IYTYSGLFC+ +NPYK  P+Y    +  Y GK+RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+
Sbjct:   14 PFLRKSEKERLEAQTRPFDLKKDVFVPDDKEE-FVKAKIVSREGGKVTA---ETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263          

BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|75054114|sp|Q8MJU9.1|MYH7_HORSE (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta)

HSP 1 Score: 1507.27 bits (3901), Expect = 0.000e+0
Identity = 805/1776 (45.33%), Postives = 1205/1776 (67.85%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ--PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            +RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I++ NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+  YLLEKSR+ FQ + ER YH FY ++S+  P+L    L+++N YDY ++SQG+ +V SIDD E++    +AF +LGF  EE  ++YKLT  +MH GNM   F    +EEQAE    + A K A + G++S  ++   C P++KVG E+VTKGQ   Q + +   +A+ +YE +F ++  + N TL   T +  QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW  +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL +N L K  NFQK  + K  P AHF+++HYA TV YN+  WL+KNKDPLN+TVV+L+K  S  +L   F ++ G   P+E  K       K    +TVS+ ++  L+ LM  L +T P F+RC++PN  K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL  AA+ + +    +  A+ +L+ + ++  ++R GHTKVFF+AG+LG +EE+R++R+  +++ +Q+Q+RG  +RM FKK+ +++ +L   Q  IR +   K W W +L+  IKP LK  +  +  A  +E+ A  ++ + K+ A RK++E     LL +K++L L +++    + D  ++  ++      ++ ++ E+  R+  E++    LT ++ K++ + S+L+ +I+ +E  LA  E++K   + +++ L EE+    E+I KL +EK+ +Q+  Q+  +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE  K +L + +++K+ E  A+NA+I+DE  LG +  K+ KELQ+R+EEL+EEL  ER +RAK EK RS L ++LE++  RLEEAG  T+ Q+E+NKKRE E  ++K ++EE  + HE T AALR KH ++++ELGEQIDNL   K K EK+K   + +L +  S++E  ++AKA L++  + ++  + +  SK +E  R++N+  SQ+ +L+ E  +L RQ++E E  ++Q  + K++ T QLED KR  + E + +++L    ++   + +  RE+ E E E K++  + LSKA +E+  W++++ET+ + R EELE  + KL  R+ +AEE VEA+N K  S EK++ RLQ ++E++ ++ ER++AAA   +K+ RNFD+++ EWK K ++   E+E+S KE R+ ++ELF+L+ A+EE++E L+  KRENKNL +EI DL +QLG  G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE  Q++ EI+RK+ EK+EE    ++NH R +DSL  SL+AE R++ EALR+KKK+E D+NE+EI L  AN+  AE QK VK  Q  L+DT    +D  R   +++E + I +R+ N L  E+EE RA++  +ERS++  + EL E+   V  + S NT  +++K+  ++ +  +Q E+++ + + +++EEKAK+A+ DAA +A+EL+ EQ+ S   +R K+ +E  + +L+ RL +AE  A K G+  + KLE R+RELE EL   Q R +ES K  +++ER +KEL +Q EEDRKN  R+ +L  KLQ K+K YK+Q EEAEE A  NL+KFRK Q E +E+E+R+  AE
Sbjct:  146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTSGKGTLEDQIIEANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYAKGALAKAVYERMFNWMVARINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSSNFQKPRNIKGKPEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVDLYKKSSLKMLSNLFANYLGADAPIEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYRFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFARLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMKRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRDAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEVEQKRNAESIKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914          

HSP 2 Score: 272.707 bits (696), Expect = 3.782e-72
Identity = 130/253 (51.38%), Postives = 174/253 (68.77%), Query Frame = 0

Query:   16 SPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            +P+L  + + + + +++P+D KK  +VPD     + +  I S +G K+T      K   V K+DQV Q NPPKFD  +DM+ LT+L++  VL+N   RY   +IYTYSGLFC+ INPYK  P+YT   +  Y GK+RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I++ NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+
Sbjct:   13 APYLRKTEKERLEDQTRPFDLKKDVFVPDDKEE-FVKAKIISREGGKITAETEHGKTVTV-KEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYAAWMIYTYSGLFCVTINPYKWLPVYTAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTSGKGTLEDQIIEANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263          

BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|83304912|sp|P12883.5|MYH7_HUMAN (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta)

HSP 1 Score: 1506.89 bits (3900), Expect = 0.000e+0
Identity = 804/1776 (45.27%), Postives = 1203/1776 (67.74%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ--PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            +RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+  YLLEKSR+ FQ + ER YH FY ++S+  P+L    L+++N YDY ++SQG+ +V SIDD E++    +AF +LGF  EE  ++YKLT  +MH GNM   F    +EEQAE    + A K A + G++S  ++   C P++KVG E+VTKGQ   Q   +   +A+ +YE +F ++  + N TL   T +  QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW  +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL +N L K  NFQK  + K  P AHF+++HYA  V YN+  WL+KNKDPLN+TVV L++  S  LL   F ++ G   P+E  K       K    +TVS+ ++  L+ LM  L +T P F+RC++PN  K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL  AA+ + +    +  A+ +L+ + ++  +++ GHTKVFF+AG+LG +EE+R++R+  +++ +Q+Q+RG  +RM +KK+ +++ +L   Q  IR +   K W W +L+  IKP LK  +  +  A  +E+    ++ + K+ A RK++E     LL +K++L L +++    + D  ++  ++      ++ ++ E+N R+  E++    LT ++ K++ + S+L+ +I+ +E  LA  E++K   + +++ L EE+    E+I KL +EK+ +Q+  Q+  +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE  K +L + +++K+ E  A+NA+I+DE  LG +  K+ KELQ+R+EEL+EEL  ER +RAK EK RS L ++LE++  RLEEAG  T+ Q+E+NKKRE E  +++ ++EE  + HE T AALR KH ++++ELGEQIDNL   K K EK+K   + +L +  S++E  ++AKA L++  + ++  + +  SK +E  R++N+  SQ+ +L+ E  +L RQ++E E  ++Q  + K++ T QLED KR  + E + +++L    ++   + +  RE+ E E E K++  + LSKA +E+  W++++ET+ + R EELE  + KL  R+ EAEE VEA+N K  S EK++ RLQ ++E++ ++ ER++AAA   +K+ RNFD+++ EWK K ++   E+E+S KE R+ ++ELF+L+ A+EE++E L+  KRENKNL +EI DL +QLG  G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE  Q++ EI+RK+ EK+EE    ++NH R +DSL  SL+AE R++ EALR+KKK+E D+NE+EI L  AN+  AE QK VK  Q  L+DT    +D  R   +++E + I +R+ N L  E+EE RA++  +ERS++  + EL E+   V  + S NT  +++K+  ++ +  +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S   +R K+ +E  + +L+ RL +AE  A K G+  + KLE R+RELE EL + Q R +ES K  +++ER +KEL +Q EEDRKN  R+ +L  KLQ K+K YK+Q EEAEE A  NL+KFRK Q E +E+E+R+  AE
Sbjct:  146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVIYATGALAKAVYERMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSANFQKPRNIKGKPEAHFSLIHYAGIVDYNIIGWLQKNKDPLNETVVGLYQKSSLKLLSTLFANYAGADAPIEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAEREKEMASMKEEFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKMELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914          

HSP 2 Score: 276.174 bits (705), Expect = 3.980e-73
Identity = 132/253 (52.17%), Postives = 175/253 (69.17%), Query Frame = 0

Query:   16 SPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            +P+L  S + + + +++P+D KK  +VPD     + +  I S +G KVT      K   V K+DQV Q NPPKFD  +DM+ LT+L++  VL+N   RY + +IYTYSGLFC+ +NPYK  P+YT   +  Y GK+RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+
Sbjct:   13 APYLRKSEKERLEAQTRPFDLKKDVFVPDDKQE-FVKAKIVSREGGKVTAETEYGKTVTV-KEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYGSWMIYTYSGLFCVTVNPYKWLPVYTPEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263          

BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|125987843|sp|P49824.3|MYH7_CANFA (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta)

HSP 1 Score: 1506.12 bits (3898), Expect = 0.000e+0
Identity = 804/1776 (45.27%), Postives = 1205/1776 (67.85%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ--PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            +RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+  YLLEKSR+ FQ + ER YH FY ++S+  P+L    L+++N YDY ++SQG+ +V SIDD E++    +AF +LGF  EE  ++YKLT  +MH GNM   F    +EEQAE    + A K A + G++S  ++   C P++KVG E+VTKGQ   Q + +   +A+ +YE +F ++  + N TL   T +  QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW  +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K  NFQK  + K    AHF+++HYA TV YN+  WL+KNKDPLN+TVV L++  S  LL   F ++ G   P+E  K       K    +TVS+ ++  L+ LM  L +T P F+RC++PN  K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL  AA+ + +    +  A+ +L+ + ++  +++ GHTKVFF+AG+LG +EE+R++R+  +++ +Q+Q+RG  SRM +KK+ +++ +L   Q  IR +   K W W +L+  IKP LK  +  +  A  +E+ A  ++ + K+ A RK++E     LL +K++L L +++    + D  ++  ++      ++ ++ E+  R+  E++    LT ++ K++ + S+L+ +I+ +E  LA  E++K   + +++ L EE+    E+I KL +EK+ +Q+  Q+  +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE  K +L + +++K+ E  A+NA+I+DE  LG +  K+ KELQ+R+EEL+EEL  ER +RAK EK RS L ++LE++  RLEEAG  T+ Q+E+NKKRE E  +++ ++EE  + HE T AALR KH ++++ELGEQIDNL   K K EK+K   + +L +  S++E  ++AKA L++  + ++  + +  SK +E  R++N+  SQ+ +L+ E  +L RQ++E E  ++Q  + K++ T QLED KR  + E + +++L    ++   + +  RE+ E E E K++  + LSKA +E+  W++++ET+ + R EELE  + KL  R+ +AEE VEA+N K  S EK++ RLQ ++E++ ++ ER++AAA   +K+ RNFD+++ EWK K ++   E+E+S KE R+ ++ELF+L+ A+EE++E L+  KRENKNL +EI DL +QLG  G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE  Q++ EI+RK+ EK+EE    ++NH R +DSL  SL+AE R++ EALR+KKK+E D+NE+EI L  AN+  AE QK VK  Q  L+DT    +D  R   +++E + I +R+ N L  E+EE RA++  +ERS++  + EL E+   V  + S NT  +++K+  ++ +  +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S   +R K+ +E  + +L+ RL +AE  A K G+  + KLE R+RELE EL + Q R +ES K  +++ER +KEL +Q EEDRKN  R+ +L  KLQ K+K YK+Q EEAEE A  NL+KFRK Q E +E+E+R+  AE
Sbjct:  146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYATGALAKAVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVALYQKSSLKLLSNLFANYAGADAPVEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEYKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFARIKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKGLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914          

HSP 2 Score: 275.789 bits (704), Expect = 5.450e-73
Identity = 132/254 (51.97%), Postives = 176/254 (69.29%), Query Frame = 0

Query:   17 PWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVF--KKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            P+L  S + + + +++P+D KK  +VPD     + +  I S +G KVT    ET++ K    K+DQV Q NPPKFD  +DM+ LT+L++  VL+N   RY + +IYTYSGLFC+ +NPYK  P+Y    +  Y GK+RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+
Sbjct:   14 PFLRKSEKERLEAQTRPFDLKKDVFVPDDKEE-FVKAKIVSREGGKVTA---ETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263          

BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|75055810|sp|Q9BE39.1|MYH7_BOVIN (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta)

HSP 1 Score: 1505.73 bits (3897), Expect = 0.000e+0
Identity = 809/1783 (45.37%), Postives = 1205/1783 (67.58%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPG--QPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIG-------KALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            +RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+  YLLEKSR+ FQ + ER YH FY ++S+  P+L    L+++N YDY ++SQG+ +V SIDD E++    +AF +LGF  EE  ++YKLT  +MH GNM   F    +EEQAE    + A K A + G++S  ++   C P++KVG E+VTKGQ   Q   +   +A+ +YE +F ++  + N TL   T +  QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW  +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL +N L K  NFQK  + K  P AHF+++HYA TV YN+  WL+KNKDPLN+TVV+L+K  S  +L   F ++ G   P+E  K       K    +TVS+ ++  L+ LM  L +T P F+RC++PN  K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL  AA+ + +    +  A+ +L  + ++  +++ GHTKVFF+AG+LG +EE+R++R+  +++ +Q+Q+RG  SRM FKK+ +++ +L   Q  IR +   K W W +L+  IKP LK        AE E++IA+ ++  G       K+ A RK++E     LL +K++L L +++    + D  ++  ++      ++ ++ E+  R+  E++    LT ++ K++ + S+L+ +I+ +E  LA  E++K   + +++ L EE+    E+I KL +EK+ +Q+  Q+  +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE  K +L + +++K+ E  A+NA+I+DE  LG +  K+ KELQ+R+EEL+EEL  ER +RAK EK RS L ++LE++  RLEEAG  T+ Q+E+NKKRE E  +++ ++EE  + HE T AALR KH ++++EL EQIDNL   K K EK+K   + +L +  S++E  ++AKA L++  + ++  + +  SK +E  R++N+  SQ+ +L+ E  +L RQ++E E  ++Q  + K++ T QLED KR  + E + +++L    ++   + +  RE+ E E E K++  + LSKA +E+  W++++ET+ + R EELE  + KL  R+ +AEE VEA+N K  S EK++ RLQ ++E++ ++ ER++AAA   +K+ RNFD+++ EWK K ++   E+E+S KE R+ ++ELF+L+ A+EE++E L+  KRENKNL +EI DL +QLG  G++IHEL+K R++LE EK ELQ+ALEEAEA+LEQEE K+LR+QLE  Q++ E++RK+ EK+EE    ++NH R +DSL  SL+AE R++ EALR+KKK+E D+NE+EI L  AN+  AE QK VK  Q  L+DT    +D  R   +++E + I +R+ N L  E+EE RA++  +ERS++  + EL E+   V  + S NT  +++K+  E+ +  +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S   +R K+ +E  + +L+ RL +AE  A K G+  + KLE R+RELE EL + Q R +ES K  +++ER +KEL +Q EEDRKN  R+ +L  KLQ K+K YK+Q EEAEE A  NL+KFRK Q E +E+E+R+  AE
Sbjct:  146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQATGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTTEEKNSMYKLTGAIMHFGNMK--FKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVVYAKGALAKAVYERMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSSNFQKPRNIKGKPEAHFSLIHYAGTVDYNIIGWLQKNKDPLNETVVDLYKKSSLKMLSSLFANYAGFDTPIEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKS-------AETEKEIALMKEEFGRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELSEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEQEEGKILRAQLEFNQIKAEMERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRLAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMEADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914          

HSP 2 Score: 276.559 bits (706), Expect = 2.692e-73
Identity = 132/255 (51.76%), Postives = 175/255 (68.63%), Query Frame = 0

Query:   14 DPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            + +P+L  S + + + +++P+D KK  +VPD     + +  I S +G KVT      K   V K+DQV Q NPPKFD  +DM+ LT+L++  VL+N   RY + +IYTYSGLFC+ INPYK  P+Y    +  Y GK+RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFA + A G R +     G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+
Sbjct:   11 EAAPYLRKSEKERLEAQTRPFDLKKDVFVPDDKEE-FVKATILSREGGKVTAETEHGKTVTV-KEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTINPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQATGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263          

BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|317373582|sp|P13533.5|MYH6_HUMAN (RecName: Full=Myosin-6; AltName: Full=Myosin heavy chain 6; AltName: Full=Myosin heavy chain, cardiac muscle alpha isoform; Short=MyHC-alpha)

HSP 1 Score: 1501.11 bits (3885), Expect = 0.000e+0
Identity = 806/1775 (45.41%), Postives = 1199/1775 (67.55%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKR------KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            +RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFAS+ A G R         + +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+  YLLEKSR+ FQ + ER YH FY ++S+  P+L    L+++N YDY +VSQG+VSV SIDD E++     AF +LGF  EE   VYKLT  +MH GNM   F    +EEQAE    ++A K A + G++S  ++   C P++KVG E+VTKGQ+  Q   S+  +A+ +YE +F ++  + N TL   T +  QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW  +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K  NFQK  + K    AHF+++HYA TV YN+  WLEKNKDPLN+TVV L++  S  L+   F  +          + GG +K    +TVS+ ++  L+ LM  L  T P F+RC++PN  K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL   A+ + +    +   + +L+ + ++  +++ GHTKVFF+AG+LG +EE+R++R+  +++ +Q+QARG+  R+ FKK+ +++ AL   Q  IR +   K W W +L+  IKP LK  +  +  A  +E+    ++ + K+ A RK++E     LL +K++L L +++    + D  ++  ++      ++ ++ E+N R+  E++    LT ++ K++ + S+L+++I+ +E  LA  E++K   + +++ L EE+    E+I KL +EK+ +Q+  Q+  +D+Q  EDK + LSK K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE  K +L + +++KE +    N+KI+DE  L  +  K+ KE Q+R+EEL+EEL  ER +RAK EK RS L ++LE++  RLEEAG  T+ Q+E+NKKRE E  +++ ++EE  + HE T AALR KH ++++ELGEQIDNL   K K EK+K   + +L +  S++E  ++AKA L++  + ++    +   KL+E  R+LN+  +Q+ +L+ E  +L RQ+EE E  ++Q  + K+S T Q+ED KR  + E + +++L    ++   + +  RE+ E E E K++  + LSKA +E+  W++++ET+ + R EELE  + KL  R+ +AEE VEA+N K  S EK++ RLQ ++E++ ++ ER++AAA   +K+ RNFD+++ EWK K ++   E+E+S KE R+ ++ELF+L+ A+EE++E L+  KRENKNL +EI DL +QLG+GG+++HEL+K R++LEVEK ELQ+ALEEAEA+LE EE K+LR+QLE  Q++ EI+RK+ EK+EE    ++NHQR +DSL  SL+AE R++ E LR+KKK+E D+NE+EI L  AN+  AE QK VK  Q  L+DT    +D  R   +++E + I +R+ N L  E+EE RA++  +ERS++  + EL E+   V  + S NT  +++K+  ES +  +Q+E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S   +R K+ +E  + +L+ RL +AE  A K G+  + KLE R+RELE EL + Q R +ES K  +++ER +KEL +Q EED+KN  R+ +L  KLQ K+K YK+Q EEAEE A  NL+KFRK Q E +E+E+R+  AE
Sbjct:  146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHYGNMK--FKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKAVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRIKETLEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQVEEDKVNSLSKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDINQQNSKIEDEQVLALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKVRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHQRVVDSLQTSLDAETRSRNEVLRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQSEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELEGELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1916          

HSP 2 Score: 269.24 bits (687), Expect = 4.383e-71
Identity = 128/242 (52.89%), Postives = 167/242 (69.01%), Query Frame = 0

Query:   30 KSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVF--KKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKR------KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            +++P+D +  C+VPD     + +  I S +G KV   I ET++ K    K+DQV Q NPPKFD  +DM+ LT+L++  VL+N   RY   +IYTYSGLFC+ +NPYK  P+Y    +  Y GK+RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFAS+ A G R         + +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+
Sbjct:   27 QTRPFDIRTECFVPDDKEE-FVKAKILSREGGKV---IAETENGKTVTVKEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLFNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 264          

BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|3024204|sp|Q02566.2|MYH6_MOUSE (RecName: Full=Myosin-6; AltName: Full=Myosin heavy chain 6; AltName: Full=Myosin heavy chain, cardiac muscle alpha isoform; Short=MyHC-alpha)

HSP 1 Score: 1495.33 bits (3870), Expect = 0.000e+0
Identity = 805/1775 (45.35%), Postives = 1198/1775 (67.49%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGE------ASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            +RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFAS+ A G R + E       +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+  YLLEKSR+ FQ + ER YH FY ++S+  P+L    L+++N YDY +VSQG+VSV SIDD E++     AF +L F  EE   VYKLT  +MH GNM   F    +EEQAE    ++A K A + G++S  ++   C P++KVG E+VTKGQ+  Q   S+  +A+ +YE +F ++  + N TL   T +  QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW  +DFGMDLQ CID+ EKPMG+++ILEEE +FPKA+D +F AKL++N L K  NFQK  + K    AHF++VHYA TV YN+  WLEKNKDPLN+TVV L++  S  L+   F  +            GG +K    +TVS+ ++  L+ LM  L  T P F+RC++PN  K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL  AA+ + +    +  A+ +L  + ++  +++ GHTKVFF+AG+LG +EE+R++R+  +++ +Q+QARG+  R+ FKK+ +++ AL   Q  IR +   K W W +L+  IKP LK  +  +  A  +E+    +  + K+ A RK++E     LL +K++L L +++    + D  ++  ++      ++ ++ E+  R+  E++    LT ++ K++ + S+L+++I+ +E  LA  E++K   + +++ L EE+    E+I KL +EK+ +Q+  Q+  +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE  K +L + +++KE + +  N+KI+DE  L  +  K+ KE Q+R+EEL+EEL  ER +RAK EK RS L ++LE++  RLEEAG  T+ Q+E+NKKRE E  +++ ++EE  + HE T AALR KH ++++ELGEQIDNL   K K EK+K   + +L +  S++E  ++AKA L++  + ++    +   KL+E  R+LN+  +Q+ +L+ E  +L RQ+EE E  ++Q  + K+S T Q+ED KR  + E + +++L    ++   + +  RE+ E E E K++  + LSKA +E+  W++++ET+ + R EELE  + KL  R+ +AEE VEA+N K  S EK++ RLQ ++E++ ++ ER++AAA   +K+ RNFD+++ EWK K ++   E+E+S KE R+ ++ELF+L+ A+EE++E L+  KRENKNL +EI DL +QLG+GG+++HEL+K R++LEVEK ELQ+ALEEAEA+LE EE K+LR+QLE  Q++ EI+RK+ EK+EE    ++NH R +DSL  SL+AE R++ EALR+KKK+E D+NE+EI L QAN+  +E QK +K  Q  L+DT    +D      +++E + I +R+ N L  E+EE RA++  +ERS++  + EL E+   V  + S NT  +++K+  ES +  +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S   +R K+ +E  + +L+ RL +AE  A K G+  + KLE R+RELE EL + Q R +ES K  +++ER +KEL +Q EED+KN  R+ +L  KLQ K+K YK+Q EEAEE A  NL+KFRK Q E +E+E+R+  AE
Sbjct:  146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLSFTAEEKAGVYKLTGAIMHYGNMK--FKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKSVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKASDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNIMGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADTGDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLKTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSSRHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNSQAHLKDTQLQLDDAVHANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLMRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1916          

HSP 2 Score: 274.248 bits (700), Expect = 1.490e-72
Identity = 129/242 (53.31%), Postives = 168/242 (69.42%), Query Frame = 0

Query:   30 KSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKV--FKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGE------ASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            +++P+D +  C+VPD     Y +  + S +G KVT    ET++ K    K+DQV Q NPPKFD  +DM+ LT+L++  VL+N   RY   +IYTYSGLFC+ +NPYK  P+Y    +  Y GK+RSE PPHIF +++ +YQ M+   +NQSILITGESGAGKT NTK+VI YFAS+ A G R + E       +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F   GKL+ AD+
Sbjct:   27 QTRPFDIRTECFVPDDKEE-YVKAKVVSREGGKVTA---ETENGKTVTIKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 264          

BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325302753|ref|XP_023332945.1| (myosin heavy chain, muscle-like [Eurytemora affinis])

HSP 1 Score: 2667.88 bits (6914), Expect = 0.000e+0
Identity = 1289/1767 (72.95%), Postives = 1537/1767 (86.98%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
            RR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVISYFA++ +SGKRKEGE SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN  GKLSGADMV+YLLEKSRLT+Q  LERCYHAFYN+MSDAVPDLK+KCLL+DNI DYW+VSQGK++V SIDDKEDMQ+A +AF ILGF +EE Y+VYK T+ +MHMGNMTKDFVPVGKEEQAEIK + N++KVA + GID EWMINYFCKPKLKVGTEWV+KG +CS A++SVAGIAR IYE  FR + DKCNETL DPTMKKVQYIG LDIAGFEIFDYNGFEQICIN+ NEKLQQFFN HMF LEQEEYVREG++WANVDFGMDLQKCIDMFEKPMGLLA+ EEESLFPKATDQ+F  KLH NLL K PNF K +P+PDP AHFAV+HYAATVSYNLT+WLEKNKDPLNDT+VE+ KNGSN+L+I+CF DHPGQPLE  KD GG  +K GG KTVSS++KGQLDDLM TLY T+P FIRCVVPNTHK+PGGVE GL+MHQYQCNGVLAGIAICRKGFPNKM Y +FKARYNILAA  VAKAK DK AA AVL  + L+ EK+RLGHTKVFFRAGILG+MEEVRED++G VL+WLQS ARGKASRM FKK+QDQKLALY CQRTIRN+ IGKTWLWWQ+W  IKP+LKCT+F +YK E+E+KIA+AE NI KA+ADR+KV+ +H+ LL QK+E++LAL SGGSAVQDIIDK  RVE +A D+QKQ+ EV  RI AE++Q+ S+  Q  KV+SQ +QL+ E++ +E +L  +EQ++ +KD+QIRTLK+E+ HQ +M+ KLG+EK+   D +QK EED+Q ++DK +HLS+VK KLEQ+LDEAEDALE EKK KND EK+KRK E D KLTQE + DLERVKAEL+Q + RKEKE++A++AKI+DE +LG KY KQ KELQ RLEELDEEL IER +RAKAEKSRS+LKKD+ED+ +RLEEAGANT TQVELNKKRE EL R+K E+EE NI HEGTLAALRMKHNNTMS+LGE ID+LN +K+KAEKDK  +ERDL ++R+SLED V+++AEL+R GK++QGSI ++++KLDE+ARA+NEA+S KKRL +E  DL RQIEE ENA+A  NK K+SL TQLEDTKRL+++E++DRSSLLTKYK++TTE E+ RE+IENEH +KSD LK+LSKAQAEIQLW+SR+ETEGMGRVEELEG   KL++RI EAEET+++L  K+ + EKS+ R+  DL+E+S+EYER HAAA+I+EKRG+NFD+V+GEW+AKA D+A E+EASN ECRNY+SEL+R++A+H+EA EQLD+VKRENKNLADEI+DLLDQLGDGGRSIH+LDKQRRRLEVEKEELQ+ALEEAE ALEQEENKVLR+QLELGQVRQEIDR++ EK+EEF NTRKNH RAMDSL+ASLEAEQ+AK EALRIKKKLE DINELEIALD ANKAN+EG K +KRYQ QLR+++QG E+ESR RQ++ E VGIA+RK  AL+ E+EESRALL S+ER KRQL+AEL ++R SVNEM SIN++ +  KRA E  +H IQAEID ++  AK+SEEK KRAMVDAARLADELR+EQ+HS +E R KR+LE+QLGELET+L +AE NA + G++A+S+LE ++RELE ELG  Q RTSE+ K +Q+ ER +KEL FQQEED+KNQ++M+E+A KLQQKIK YKQQIEEAEEIAALNLAKFRK+QQ+FEE+E+R K A+++
Sbjct:  149 RRNECPPHIFAIAEGAYQGMMNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKRKEGEVSLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADMVVYLLEKSRLTYQQPLERCYHAFYNIMSDAVPDLKEKCLLTDNILDYWYVSQGKITVPSIDDKEDMQFADEAFDILGFTKEEKYDVYKNTACMMHMGNMTKDFVPVGKEEQAEIKDDTNSIKVATLAGIDPEWMINYFCKPKLKVGTEWVSKGSSCSNAANSVAGIARAIYERTFRLVVDKCNETLIDPTMKKVQYIGVLDIAGFEIFDYNGFEQICINYVNEKLQQFFNSHMFTLEQEEYVREGLDWANVDFGMDLQKCIDMFEKPMGLLAVFEEESLFPKATDQTFCEKLHSNLLGKWPNFAKPNPRPDPDAHFAVIHYAATVSYNLTNWLEKNKDPLNDTIVEMIKNGSNSLMIQCFADHPGQPLETPKDDGGRKKKGGG-KTVSSYFKGQLDDLMTTLYKTEPHFIRCVVPNTHKQPGGVEPGLIMHQYQCNGVLAGIAICRKGFPNKMIYAEFKARYNILAAKLVAKAKNDKAAAGAVLESINLDKEKYRLGHTKVFFRAGILGFMEEVREDKIGSVLAWLQSGARGKASRMAFKKLQDQKLALYCCQRTIRNFRIGKTWLWWQIWSAIKPHLKCTQFGKYKEEFEQKIALAEANIDKAIADRQKVQAIHDNLLSQKNEVSLALTSGGSAVQDIIDKAVRVENIANDIQKQVAEVKKRIKAEEEQKSSIEDQMRKVESQINQLQSEVSTLERQLENSEQERSDKDDQIRTLKDEIAHQADMLAKLGKEKKSGIDFRQKVEEDIQTMDDKCNHLSRVKGKLEQALDEAEDALEREKKSKNDVEKLKRKCEGDFKLTQEAVVDLERVKAELAQTLARKEKEYSALSAKIEDEGSLGSKYQKQIKELQGRLEELDEELNIERSNRAKAEKSRSILKKDIEDIAARLEEAGANTGTQVELNKKREGELSRLKCELEELNIAHEGTLAALRMKHNNTMSDLGENIDSLNSSKVKAEKDKSGLERDLADSRASLEDAVKSRAELERTGKLLQGSIAESHTKLDELARAMNEADSTKKRLTVENQDLSRQIEELENALANMNKSKVSLATQLEDTKRLSESESKDRSSLLTKYKHMTTEFETYREKIENEHLKKSDALKSLSKAQAEIQLWRSRYETEGMGRVEELEGNNSKLRSRITEAEETIDSLQTKLANIEKSKQRIGEDLDEISMEYERVHAAALITEKRGKNFDKVLGEWQAKASDIACEVEASNNECRNYSSELYRVKASHDEATEQLDIVKRENKNLADEIKDLLDQLGDGGRSIHDLDKQRRRLEVEKEELQSALEEAEGALEQEENKVLRAQLELGQVRQEIDRRLAEKDEEFLNTRKNHDRAMDSLNASLEAEQKAKGEALRIKKKLEGDINELEIALDHANKANSEGMKTIKRYQSQLRESLQGLENESRIRQQIAEQVGIAERKAAALSAEMEESRALLDSAERGKRQLEAELADTRNSVNEMQSINSKEIAVKRALEGNLHTIQAEIDSLLQAAKSSEEKGKRAMVDAARLADELRAEQDHSAAESRIKRSLETQLGELETKLTEAEENAIRCGKSALSRLETKVRELEQELGCTQVRTSENMKGFQKTERKIKELLFQQEEDKKNQDKMSEIANKLQQKIKVYKQQIEEAEEIAALNLAKFRKAQQDFEENEERVKLADEA 1914          

HSP 2 Score: 478.019 bits (1229), Expect = 2.733e-134
Identity = 209/263 (79.47%), Postives = 232/263 (88.21%), Query Frame = 0

Query:    1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            MPGHIKKS GPDPDPSPWL VSPELK K K KPYDPKKS WV +KA GGY EGL++S DG KVTV +    + KV+K+DQV QVNPPKFDCSDDM+ LT+L DACVLWNS +RY N+LIYTYSGLFCIAINPYKRFPIYT R++++Y+G+RR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVISYFA++ +SGKRKEGE SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN  GKLSGADM
Sbjct:    1 MPGHIKKSSGPDPDPSPWLCVSPELKEKNKLKPYDPKKSVWVVNKADGGYLEGLLESKDGAKVTVNV--NGEMKVYKEDQVCQVNPPKFDCSDDMANLTFLGDACVLWNSTIRYVNQLIYTYSGLFCIAINPYKRFPIYTLRTMEIYVGRRRNECPPHIFAIAEGAYQGMMNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKRKEGEVSLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADM 261          

BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325302843|ref|XP_023332992.1| (myosin heavy chain, muscle-like [Eurytemora affinis])

HSP 1 Score: 2655.55 bits (6882), Expect = 0.000e+0
Identity = 1283/1765 (72.69%), Postives = 1517/1765 (85.95%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            RR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVI+YFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN  GKLSGADMV+YLLEKSRLT+Q  LERCYHAFYN+MSDAVPDLK+KCLLSDNI DYW+VSQGK+SV SIDDKEDM +A +AF +LGF E+E Y+VYK T+ +MHMGNMTKDFVPVGKEEQAEIK + NA+KVA + GID+EWMINYFCKPKLKVGTEWV KG TCS A++SVAGIAR IYE  FR + +KCNETL DPTMKKVQYIG LDIAGFEIFDYNGFEQICIN+ NEKLQQFFNQHMF LEQEEYVREG++WANVDFGMDLQKCIDMFEKPMGLLAI EEESLFPKATDQSF  KLH NLL K  NF K +P+PDP AHFAV+HYAA VSYNLT WLEKNKDPLNDT+VE+ KNGSN+L+I+CF DHPGQPLE  KD GG  +K GG KTVSS++KGQLDDLM TLY T+P FIRCVVPNTHK+PGGVE GLVMHQYQCNGVLAGIAICRKGFPNKM Y +FKARYNILAA AVAK K DK AA AVL+ +KLE EK+RLGHTKVFFRAGILG+MEEVRED++GEVLSWLQ+ ARGKASRM FKK+QDQKLALY CQRTIRNYYIGKTWLWWQ+W+L+KP+LKC +F +YKAEYE+KIA+AE NI KA+A+   V  +H+ L  +K EL LAL SGGSAVQDIIDK  R+EGM  D+QKQ+DE   RIAAE+     + Q   KV +   +LREEI  +E      E+D++ KD QI+TLKEE+ HQ E+I K+ REK+ V D +QK EED+Q++ED+ +HL+KVK KLEQSLDE EDALE EKK + D EK+KRKIE DLKLTQE +SDLERVKAELSQ +QRKEKE ++++AKI+DE TLGGKYSKQ KELQSR+EELDEEL IER +RAKAEK+RS+L +D+ED+G RLEEAG NT+TQ+ELNKKRE EL ++K+++EE NI HEGTLAA+R KHNNTMS++GEQID+LN  K K++KDK  MERDLQEAR+ L++ +R +A +++N KM Q  I ++N+KLDE+ARALNEA+S  K+L +E  DL RQIEE ENA+A   K K+SLTTQLEDTKRLADAE RDR+SLLTK+K L+TE+E+ R RIE E ERK+D L+ALSKAQAEIQLW+S+FETE +GR++ELE ++ KL  R+ EAEET+++LN K+ ++EK++ R++ +LEEM +EYERTHAAA+I+EKRGRNFD+V+GEWKAKADDL  E+EAS  ECRNYNSE+FRL+AA+EE +EQLDVV+RENKNLADEI+DLLDQLGDGGRSIH+LDKQRRRLEVEKEELQAALEEAE+ALEQEENKVLR+QLELGQV+QEIDR+IQEKEEEF+NTRKNH RAMDS+ ASLE+EQRAK EALRIKKK+E DINELEIALD ANKAN E QK++KRYQGQLR++   YE+ESR R E+ E   +ADR+ NAL GE+EE+R+LL S+ER K+Q +AEL ++R +VNEM++IN++A  EKR  E  +H + AEIDDM+ QAKNSEEK+K+AM+DAARLADELR+EQ+H  ++ + KRA E+QL ELE +L +A  NA + GRAAM+KLE RIRELE+ELGS+QSRTS++AK +Q+ ER VKEL FQ +ED+KNQERM+ELA KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EESE+R++ AE
Sbjct:  149 RRNECPPHIFAIAEGAYQGMMNSGCNQSILITGESGAGKTENTKKVIAYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADMVVYLLEKSRLTYQQPLERCYHAFYNIMSDAVPDLKEKCLLSDNILDYWYVSQGKISVPSIDDKEDMMFADEAFDVLGFTEDEKYDVYKNTACMMHMGNMTKDFVPVGKEEQAEIKDDTNAIKVAALAGIDAEWMINYFCKPKLKVGTEWVNKGSTCSAAANSVAGIARAIYERSFRIVVEKCNETLIDPTMKKVQYIGVLDIAGFEIFDYNGFEQICINYVNEKLQQFFNQHMFTLEQEEYVREGLDWANVDFGMDLQKCIDMFEKPMGLLAIFEEESLFPKATDQSFCEKLHSNLLGKWENFAKPNPRPDPDAHFAVIHYAAVVSYNLTGWLEKNKDPLNDTIVEMIKNGSNSLMIQCFADHPGQPLETPKDDGGRKKKGGG-KTVSSYFKGQLDDLMTTLYKTEPHFIRCVVPNTHKQPGGVEPGLVMHQYQCNGVLAGIAICRKGFPNKMIYAEFKARYNILAAKAVAKCKNDKSAAGAVLDAIKLEKEKYRLGHTKVFFRAGILGFMEEVREDKIGEVLSWLQAGARGKASRMQFKKLQDQKLALYCCQRTIRNYYIGKTWLWWQIWMLVKPHLKCMQFGKYKAEYEKKIAVAEANIDKAIAECDAVIKIHDRLENEKQELHLALTSGGSAVQDIIDKTNRLEGMKNDLQKQVDETKKRIAAEEDVIAGIQQAGNKVTADAHRLREEIKNLENAAEKCEEDRITKDNQIKTLKEEISHQEELIAKMQREKKSVGDGRQKVEEDIQSMEDRCNHLAKVKGKLEQSLDECEDALEREKKCRGDVEKMKRKIEGDLKLTQEAVSDLERVKAELSQTIQRKEKELSSLSAKIEDEQTLGGKYSKQIKELQSRIEELDEELAIERQNRAKAEKNRSILSRDIEDIGRRLEEAGCNTSTQIELNKKRESELVKLKSDLEEANIAHEGTLAAIRAKHNNTMSDMGEQIDSLNKMKAKSDKDKAGMERDLQEARAGLDESMRDRANIEKNCKMTQALIVESNTKLDELARALNEADSSNKKLSVEHQDLIRQIEETENAIATLGKNKVSLTTQLEDTKRLADAEGRDRASLLTKFKGLSTEVENLRMRIEEEAERKNDVLRALSKAQAEIQLWRSKFETEALGRIDELESSKAKLNVRVQEAEETIDSLNTKVSATEKTKHRIEAELEEMQMEYERTHAAAVITEKRGRNFDKVVGEWKAKADDLLAELEASRTECRNYNSEVFRLKAAYEETVEQLDVVRRENKNLADEIKDLLDQLGDGGRSIHDLDKQRRRLEVEKEELQAALEEAESALEQEENKVLRAQLELGQVKQEIDRRIQEKEEEFDNTRKNHARAMDSMQASLESEQRAKAEALRIKKKIEGDINELEIALDHANKANNEAQKSIKRYQGQLRESECAYEEESRMRAEMTEKACLADRRANALHGEMEEARSLLDSAERGKKQTEAELADARNAVNEMNTINSKAASEKRRVEGIVHTMHAEIDDMLQQAKNSEEKSKKAMIDAARLADELRAEQDHVNTQSKAKRAFETQLVELENKLDEANENAMRGGRAAMAKLETRIRELEIELGSVQSRTSDNAKGHQKCERRVKELSFQIDEDKKNQERMSELATKLQQKIKTYKKQIEEAEEIAALNLAKFRKAQQELEESEERTRMAE 1912          

HSP 2 Score: 486.108 bits (1250), Expect = 5.331e-137
Identity = 213/263 (80.99%), Postives = 236/263 (89.73%), Query Frame = 0

Query:    1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            MPGHIKK+ GPDPDPSPWLIVSPELK K K+KPYDPKKS WVP+KA GG+ EGLI+S DG K++V +    + KVFK+DQV QVNPPKFDCSDDM+ LTYL DACVLWNSV+RY NELIYTYSGLFCIAINPYKRFPIYT R+++LY GKRR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVI+YFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN  GKLSGADM
Sbjct:    1 MPGHIKKTTGPDPDPSPWLIVSPELKEKNKAKPYDPKKSVWVPNKADGGFVEGLIESKDGAKISVNV--KGEVKVFKEDQVCQVNPPKFDCSDDMANLTYLGDACVLWNSVIRYVNELIYTYSGLFCIAINPYKRFPIYTYRTMELYTGKRRNECPPHIFAIAEGAYQGMMNSGCNQSILITGESGAGKTENTKKVIAYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADM 261          

BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325305439|ref|XP_023334330.1| (myosin heavy chain, muscle-like [Eurytemora affinis])

HSP 1 Score: 2631.28 bits (6819), Expect = 0.000e+0
Identity = 1269/1765 (71.90%), Postives = 1518/1765 (86.01%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            RR+ECPPHIF +AEG+YQGM+N G NQSILITGESGAGKTENTKKVISYFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN  GKLSGADMV+YLLEKSRLT+Q  LERCYHAFYNLMSD VPDLK+KCLL+D+I DYW+VSQGK+SVESI+DKEDMQ+A +AF ILGF+++E Y+V+K T+ +MHMGNMTKDFVPVGK+EQAEIK E NA  VA + GID+EWMI YFCKPKLKVGTEWV KG +C+ A+SSVAGIAR IYE  FR + +KCNETL DPTMKKVQYIG LDIAGFEIFD+NGFEQICIN+ NEKLQQFFNQHMF LEQEEYVREG++WANVDFGMDLQKCIDMFEKPMGLLA+ EEESLFPKATDQ+F  KLH NLL K PNF K +P+PDP AHFAV+HYAATVSYNLTSWLEKNKDPLNDT+VE+ KNGSN+L+I+CF DHPGQPLEA KD GG  +K GG KTVSS++KGQLDDLM TLY T+P FIRCVVPNTHK+PGGVE GL+MHQYQCNGVLAGIAICRKGFPNKM Y +FKARYNILAA  VAKAK DK AA AVL  ++LE EK+RLGHTKVFFRAGILG+MEEVREDR+G+VL+WLQS ARGK+SRM FKK+QDQKLALY CQRTIRNYYIGKTWLWWQ+W+ IKPNLKCT+F +YKAEYE KIA+AE NI KA+A+  KV   H+ LL +K+EL LAL SGG+AVQDIIDK  R+E    D+QKQ+D+ N RI  E++    + Q   KV  + ++LR+EI  +E  +   E+DK+ KD QIRTL++E+ HQ ++I KL +EK+   + +QK EED+Q++ED+ +HL+KVK KLEQSLDE ED LE EKK K D EK+KRK+E DLKLTQE  SDLER+ AEL+Q VQRKEKE A++ AKI+DE TLGGKYSKQ KELQSR++ELDEE+++ER +RAKAEK+RS+L +DLED+G+RL EAG+NT+TQ+ELNKKRE ELH++KA++EE NI HEGTLAALR KHNN+MSELGEQID++N NK K+EKDK  MERDLQEAR+ LE+ +R +A +++N KM Q  I ++N KLDE+ARALNEA+S KK+L +E  DL RQI+E ENA+A   K KISLTTQLEDTKRLAD EARDR++LLTKYKNL+T+ E+ R +I+ E E+K+D L+ALSKAQAEIQLW+S+FETEG+GR+EELEG++ KL AR+ EAEE +E+LN+KI S+EKS+ R++ +LEEM++EYERTHAAA+I+EKRGRNFD+V+GEWKAKADD+  E+EAS+ ECRN+N+E FRL+AA +E+ EQLD+V+RENKNLADEI+DLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAE ALE EENKVLR+QLELGQVRQEIDR+I EKEEEFNN+RKNHQRAM+S+ ASLE+EQRAK+EALRIKKKLE DINELEIALD +NKAN E QK++KRYQ QLR++   YE+ SR RQE+ E   +A+R+ NAL GE+EE+R+LL S+ER KRQ +AEL E+R +VNEM++IN+RA  EKR  E   H +QAEIDDM+ QAKNSEEK+K+AMVDAARLADELR+EQ+H  ++ + KRALE+Q+ ELE  LA+A  +A + GR AM+KLE R+RELE+ELG++Q RT ++AKA+Q+AER +KELQFQ +ED KNQERM++LA KLQ KIKTYK+QIEEAEEIAALNLAKFRKSQQE EE+E+R + A+
Sbjct:  149 RRNECPPHIFAIAEGAYQGMLNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNAAGKLSGADMVVYLLEKSRLTYQQPLERCYHAFYNLMSDQVPDLKEKCLLTDDIRDYWYVSQGKLSVESINDKEDMQFADEAFDILGFSQDEKYDVFKNTACMMHMGNMTKDFVPVGKDEQAEIKDEKNANIVATLMGIDAEWMITYFCKPKLKVGTEWVQKGSSCNNAASSVAGIARAIYERTFRLVVEKCNETLCDPTMKKVQYIGVLDIAGFEIFDFNGFEQICINYVNEKLQQFFNQHMFTLEQEEYVREGLDWANVDFGMDLQKCIDMFEKPMGLLAVFEEESLFPKATDQTFCEKLHANLLGKWPNFAKPNPRPDPDAHFAVLHYAATVSYNLTSWLEKNKDPLNDTIVEMIKNGSNSLMIQCFLDHPGQPLEAPKDDGGRKKKGGG-KTVSSYFKGQLDDLMTTLYKTEPHFIRCVVPNTHKQPGGVEPGLIMHQYQCNGVLAGIAICRKGFPNKMIYAEFKARYNILAAKLVAKAKNDKGAAGAVLEAIQLEKEKYRLGHTKVFFRAGILGFMEEVREDRIGQVLAWLQSGARGKSSRMQFKKLQDQKLALYCCQRTIRNYYIGKTWLWWQMWMAIKPNLKCTQFGKYKAEYEGKIALAEANIDKAIAECNKVVAEHDRLLNEKNELDLALNSGGNAVQDIIDKTNRIEAARNDLQKQVDQTNARIRGEEETIAGIQQSGIKVTGEATKLRDEIKNLENTIGKCEEDKMTKDNQIRTLRDEIAHQEDLIAKLSKEKKSTGEGRQKIEEDIQSMEDRCNHLNKVKGKLEQSLDECEDTLEREKKAKGDVEKMKRKVEGDLKLTQEATSDLERINAELAQTVQRKEKESASLYAKIEDEQTLGGKYSKQVKELQSRIDELDEEIIVERNNRAKAEKNRSLLSRDLEDIGTRLAEAGSNTSTQIELNKKREAELHKLKADLEEANIAHEGTLAALRQKHNNSMSELGEQIDSINKNKAKSEKDKAGMERDLQEARTGLEEAMRERANMEKNCKMTQSLIVESNQKLDELARALNEADSTKKKLMVESQDLSRQIDETENAIAALQKNKISLTTQLEDTKRLADGEARDRAALLTKYKNLSTDAENLRMKIDEESEKKNDVLRALSKAQAEIQLWRSKFETEGLGRIEELEGSKHKLAARVSEAEEAIESLNSKISSAEKSKHRIESELEEMAMEYERTHAAAVITEKRGRNFDKVVGEWKAKADDIQAELEASHSECRNFNAEGFRLKAALDESNEQLDIVRRENKNLADEIKDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEGALESEENKVLRAQLELGQVRQEIDRRIAEKEEEFNNSRKNHQRAMESMQASLESEQRAKSEALRIKKKLEGDINELEIALDHSNKANNEAQKSIKRYQCQLRESECAYEEASRVRQEMSEKASLAERRANALQGEMEEARSLLDSAERGKRQTEAELAEARNAVNEMTTINSRASAEKRHIEGNCHTMQAEIDDMLHQAKNSEEKSKKAMVDAARLADELRAEQDHVNTQAKAKRALETQMNELEQNLAEANEHAMRGGRTAMAKLETRVRELEIELGNVQGRTGDNAKAHQKAERKIKELQFQNDEDHKNQERMSDLASKLQAKIKTYKKQIEEAEEIAALNLAKFRKSQQELEETEERVRMAD 1912          

HSP 2 Score: 479.559 bits (1233), Expect = 9.333e-135
Identity = 210/263 (79.85%), Postives = 233/263 (88.59%), Query Frame = 0

Query:    1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            MPGHIKK+ GPDPDPSPWL+VSPELK K K KPYDPKKS WV +KA GGY EGL++S DG+K TV +    + KVFK+DQV QVNPPKFDCSDDM+ LTYL DACVLWNS +RY N+LIYTYSGLFCIAINPYKRFPIYT R+++LY GKRR+ECPPHIF +AEG+YQGM+N G NQSILITGESGAGKTENTKKVISYFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN  GKLSGADM
Sbjct:    1 MPGHIKKTTGPDPDPSPWLVVSPELKDKNKLKPYDPKKSVWVKNKADGGYLEGLLESKDGNKWTVNV--KGEMKVFKEDQVCQVNPPKFDCSDDMANLTYLGDACVLWNSTIRYVNQLIYTYSGLFCIAINPYKRFPIYTLRTMELYTGKRRNECPPHIFAIAEGAYQGMLNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNAAGKLSGADM 261          

BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325281011|ref|XP_023325178.1| (myosin heavy chain, muscle-like [Eurytemora affinis] >gi|1325281013|ref|XP_023325179.1| myosin heavy chain, muscle-like [Eurytemora affinis] >gi|1325281015|ref|XP_023325180.1| myosin heavy chain, muscle-like [Eurytemora affinis] >gi|1325281017|ref|XP_023325181.1| myosin heavy chain, muscle-like [Eurytemora affinis])

HSP 1 Score: 2404.02 bits (6229), Expect = 0.000e+0
Identity = 1216/1767 (68.82%), Postives = 1473/1767 (83.36%), Query Frame = 0

Query:  264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSED--NAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
            RR+E PPHIF +AEG++QGMM  GKNQSILITGESGAGKTENTKKVISYFAS+GA+GK+KEGE  LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FNQ GKL+GADMVIYLLEKSRLT+Q  LERCYH+FYNLMSDAVPDLK KCLLS+N+ DY++VSQGK++V+SIDDKEDMQ+A +AF ILGF +EE Y+VYK T+ +MHMG + K F+ VGKEEQA+IK ED  +A  V+ + GID+EWMINYFCKPKLKVG EWVTKGQT SQASSSVAGIAR +YE  F+FI DKCN+TL DPTMK+VQ+IG LDIAGFEIFDYNGFEQICINFCNEKLQQFFN HMFVLEQEEY++EGIEWA VDFGMDLQKCI+MFEKPMGLLAILEEESLFPKATDQSFAAKLH NLL KCPNF K  PKPDP AHFAV HYAA VSYNLTSWLEKNKDPLNDT+VE+ KNG+N L + CF DHPGQP E  KD GG  +K GG KTVSSFYK QL+DLMKTL+AT+P FIRCVVPNTHK+PGG+E  L+MHQ  CNGVL GI ICRKGFPN+M+Y DFK+RYNILAA  VAKAK DK AA AVL  V LE EK+RLGHTKVFFRAG+LG+MEEVREDRVG+VLSWLQS  RGK SRM F KMQ QKL+LY CQRTIRNY IGKTWLWWQ+W+ +KPNLKCTKF+QYKAEYEEKIAIAE NI KA+A+  KV++ H  L  +K+++  +L+SGG  V+++  K  ++E    D++KQ+D++N RI +E+  R S  Q   K++    +LR+EI  ++T +  +E+D V K+ QIRTLKEEL HQ E+I KL REK+   +++QKTEE +QA EDK +HLSK+K KLEQSLDE+ED+LE EKK + D+EK+K+K+ESDLKLTQET++DL+R+K EL+  +QRKEKE+A++ AKI+DE TLGGKYSKQ KELQ+R+EELDEEL IER +R KAEK+R+ L +D+EDLG +LE+AG NT TQ+ELNKKRE EL ++KA++EE NI  EG LA  R KHN  +SE+G QID+LN  K KAE DK  MERDL EA++SL+  +R +  +++ GK+ Q  I ++N +LD+MARALNEA+S KK+L +E  DL+RQIE+ E  +A+  K KISLTTQLEDT+RLADAE RDR+ L++K+KNL +ELE+ R RIE E E K + LK LSKAQ+EIQLWKS++ETEG+GR++ELEG + KL  R+ EAEET+E+L+ K+ S+EKS+ R+  +LE++ LE+ER +AAAI+++KR +NFD+V+GEWK K DDL+ E+EAS +E RNYNSE+FRLRA  EE  EQLD VKRENKNLA+EI+DLLDQLG+GGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKV+R+QLEL QVRQEIDR+IQEKEEEF NTRKN+QRA+DS+ AS+EAE RAK EALRIKKKLE DINELEIALD ANK+NAE  K +KRYQ Q R+    YE+ESR RQE+ +  G+ADRK NAL GE+EESR+LL S+ER K+Q + EL ++R +VN+M++IN RA +EKR  ES +H + AEID ++   KNSEEKAK+AMVDA+RLA+ELRSEQ+H +S+ + +RAL++Q+ ELE RL +   +A K G+ A++KLE RIRELEMELG+ Q RTSE+ K+YQ++ER +KELQFQ +ED+KNQE+M ELA KLQ KI+TYK+QIEEAEEIAALNLAKFRK+QQE EE+E+RS+ AE
Sbjct:  149 RRNEVPPHIFAIAEGAFQGMMVGGKNQSILITGESGAGKTENTKKVISYFASIGATGKKKEGEPGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNQSGKLAGADMVIYLLEKSRLTYQQPLERCYHSFYNLMSDAVPDLKAKCLLSNNVRDYFYVSQGKIAVDSIDDKEDMQFADEAFDILGFTKEEKYDVYKNTACLMHMGELAKSFIGVGKEEQADIKEEDQKHAKNVSTLLGIDNEWMINYFCKPKLKVGAEWVTKGQTVSQASSSVAGIARSLYERTFKFIVDKCNDTLCDPTMKRVQFIGVLDIAGFEIFDYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYMKEGIEWAMVDFGMDLQKCIEMFEKPMGLLAILEEESLFPKATDQSFAAKLHANLLGKCPNFAKPDPKPDPEAHFAVCHYAAKVSYNLTSWLEKNKDPLNDTIVEMIKNGTNALCVLCFLDHPGQPAETPKDQGGSKKKGGG-KTVSSFYKSQLEDLMKTLHATEPHFIRCVVPNTHKQPGGIEPALIMHQLTCNGVLEGIRICRKGFPNRMSYPDFKSRYNILAAGLVAKAKNDKAAASAVLETVGLEKEKYRLGHTKVFFRAGVLGHMEEVREDRVGQVLSWLQSYCRGKQSRMNFVKMQGQKLSLYCCQRTIRNYMIGKTWLWWQIWITLKPNLKCTKFAQYKAEYEEKIAIAEANIDKAIAECNKVKSAHAKLDEEKADVKRSLESGGDVVKELSAKIEKLEKSRNDLEKQVDQLNTRIRSEEDTRRSAEQTGSKLKKDTDKLRDEIRDVQTNIEKSEEDVVTKESQIRTLKEELAHQEELIQKLTREKKTAGESRQKTEEGIQAAEDKCNHLSKLKLKLEQSLDESEDSLEREKKARADAEKIKKKVESDLKLTQETVADLDRLKEELNSTIQRKEKEFASVAAKIEDEQTLGGKYSKQIKELQTRIEELDEELAIERNNRNKAEKNRATLSRDIEDLGEKLEDAGNNTQTQIELNKKREAELMKLKADMEESNISFEGILANTRSKHNGVISEMGSQIDDLNKAKAKAEADKARMERDLHEAKTSLDAAIRERQNVEKEGKLTQSKIAESNQRLDDMARALNEADSSKKKLTVEGQDLQRQIEDSEAHIAELGKAKISLTTQLEDTRRLADAETRDRAGLMSKFKNLNSELENLRARIEEEAEHKGEILKQLSKAQSEIQLWKSKYETEGLGRIDELEGNKAKLMMRLQEAEETIESLHTKVASTEKSKHRMDTELEDLQLEFERVNAAAIVADKRAQNFDKVVGEWKMKVDDLSSELEASQRESRNYNSEVFRLRAGWEEITEQLDAVKRENKNLAEEIKDLLDQLGEGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVMRAQLELSQVRQEIDRRIQEKEEEFENTRKNYQRALDSMQASMEAETRAKAEALRIKKKLEADINELEIALDHANKSNAEAHKTIKRYQNQYREAETAYEEESRQRQEIVDKAGLADRKANALLGELEESRSLLDSAERGKKQAELELHDARVAVNDMTTINGRAANEKRQLESAVHTLHAEIDSILQSVKNSEEKAKKAMVDASRLAEELRSEQDHCSSQLKARRALDAQVKELELRLEEVVEHATKGGKNAIAKLEGRIRELEMELGNSQMRTSETLKSYQKSERKIKELQFQNDEDQKNQEKMGELATKLQDKIRTYKKQIEEAEEIAALNLAKFRKAQQELEEAEERSRLAE 1914          

HSP 2 Score: 477.248 bits (1227), Expect = 4.564e-134
Identity = 209/263 (79.47%), Postives = 236/263 (89.73%), Query Frame = 0

Query:    1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            MPGHIKK  GPDPDPS WL VS ELK K K+KPYDPKKSCWVP+KA+GG+ EG+I+ST+GDK+TV++    +KKVFKKD VGQVNPPKFDC +DMS LTYLNDA VLWN  +RY NELIYTYSGLFCIA+NPYKRFPIYTQR++++Y+GKRR+E PPHIF +AEG++QGMM  GKNQSILITGESGAGKTENTKKVISYFAS+GA+GK+KEGE  LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FNQ GKL+GADM
Sbjct:    1 MPGHIKKGSGPDPDPSQWLFVSYELKEKNKAKPYDPKKSCWVPEKATGGFAEGMIESTEGDKITVSV--KGEKKVFKKDLVGQVNPPKFDCCEDMSNLTYLNDASVLWNLRIRYVNELIYTYSGLFCIAVNPYKRFPIYTQRAMEVYMGKRRNEVPPHIFAIAEGAFQGMMVGGKNQSILITGESGAGKTENTKKVISYFASIGATGKKKEGEPGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNQSGKLAGADM 261          

BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022994|ref|XP_023023736.1| (myosin heavy chain, muscle isoform X48 [Leptinotarsa decemlineata])

HSP 1 Score: 1898.63 bits (4917), Expect = 0.000e+0
Identity = 992/1789 (55.45%), Postives = 1339/1789 (74.85%), Query Frame = 0

Query:  250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
            ++ N+  KL     RR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+  YLLEK+R+  Q  LER YH FY +MS AV  +K KCLLS+NI DY++VSQGK ++  +DD E+M+   +AF ILGF +EE  NVYK+T+ VMHMG M   F   G+EEQAE    +   +V ++ G+D+  +   F KP++KVG E+VT+G+  +Q S SV  +++ +++ VF+F+  KCNETL D   K+  +IG LDIAGFEIFD+NGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGIEWA +DFGMDL  CI++ EKPMG+L+ILEEES+FPKATD++F  KL+ N L K PNFQK  P P P    AHF + HYA  V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ   A +   GG R KG   +TVSS Y+ QL++LM TL +T P F+RC++PN  K+PG ++S LVMHQ  CNGVL GI ICRKGFPN+M Y DFK RY IL+A+A+      + AA+ +L+ + L+ E++RLG TKVFFRAG+LG MEE+R++R+G++++W+QS  RG  SR  FKK+Q+Q+LAL  CQR +R Y   +TW W++LW  ++P L  T+     A+ EEK A A++   +    +K++E ++  LL +K+EL   L+    ++ ++ ++  +++    D++ QL E  +R++ E+  R+ L QQ+ K++ + S  +++I  +E  L  +EQDK  KD QIR L +E+ HQ E+I KL +EK+   +N QK  E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK  KQ KELQ+R+EEL+EE+  ER +RAKAEK R+ L ++LE+LG RLEEAG  T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN  K KAE+D+ N+  +LQ+ R ++E   R KA +++  K +   + D   KLDE  R LN+ ++ KK+L +E  DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL  +L++ RE++E E E K+D  + LSKA AE QLW+ ++E+EG+ R EELE  + KLQAR+ EAEET+E+LN K+V+ EK++ RL  ++E++ LE +R +A A  +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE  EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT    E+E R R E +E +GI++R+ NAL  E+EESR LL  ++R++RQ + EL ++   +N++ + N      KR  E+ +  + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL   Q R +++ K  +++ER +KEL FQ EEDRKN ERM +L  KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+  AE++
Sbjct:  135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWAFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILSASAIRDNMTPEKAAQVILDHINLDPEQYRLGKTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916          

HSP 2 Score: 353.214 bits (905), Expect = 1.987e-94
Identity = 166/261 (63.60%), Postives = 200/261 (76.63%), Query Frame = 0

Query:    7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            + +G DPDP+P+L VS E K   ++KPYD KKSCWVPD   G +  G I  T GD VTV  L   ++K FKKDQV QVNPPK++  +DMS LTYLNDA VL N   RY  +LIYTYSGLFC+AINPYKRFP+YT R   LY GKRR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+
Sbjct:    6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264          

BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022910|ref|XP_023023693.1| (myosin heavy chain, muscle isoform X6 [Leptinotarsa decemlineata])

HSP 1 Score: 1896.32 bits (4911), Expect = 0.000e+0
Identity = 992/1789 (55.45%), Postives = 1334/1789 (74.57%), Query Frame = 0

Query:  250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
            ++ N+  KL     RR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+  YLLEK+R+  Q  LER YH FY +MS AV  +K KCLLS+NI DY++VSQGK ++  +DD E+M+   +AF ILGF +EE  NVYK+T+ VMHMG M   F   G+EEQAE    +   +V ++ G+D+  +   F KP++KVG E+VT+G+  +Q S SV  +++ +++ VF+F+  KCNETL D   K+  +IG LDIAGFEIFDYNGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGI W  +DFGMDL  CI++ EKPMG+L+ILEEES+FPKATD++F  KL+ N L K PNFQK  P P P    AHF + HYA  V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ   A +   GG R KG   +TVSS Y+ QL++LM TL +T P F+RC++PN  K+PG ++S LVMHQ  CNGVL GI ICRKGFPN+M Y DFK RY IL  A V+K    K   + +L+   L++E +RLGHTKVFFRAG+LG MEE+R++R+G++++W+QS  RG  SR  FKK+Q+Q+LAL  CQR +R Y   +TW W++LW  ++P L  T+     A+ EEK A A++   +    +K++E ++  LL +K+EL   L+    ++ ++ ++  +++    D++ QL E  +R++ E+  R+ L QQ+ K++ + S  +++I  +E  L  +EQDK  KD QIR L +E+ HQ E+I KL +EK+   +N QK  E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK  KQ KELQ+R+EEL+EE+  ER +RAKAEK R+ L ++LE+LG RLEEAG  T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN  K KAE+D+ N+  +LQ+ R ++E   R KA +++  K +   + D   KLDE  R LN+ ++ KK+L +E  DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL  +L++ RE++E E E K+D  + LSKA AE QLW+ ++E+EG+ R EELE  + KLQAR+ EAEET+E+LN K+V+ EK++ RL  ++E++ LE +R +A A  +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE  EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT    E+E R R E +E +GI++R+ NAL  E+EESR LL  ++R++RQ + EL ++   +N++ + N      KR  E+ +  + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL   Q R +++ K  +++ER +KEL FQ EEDRKN ERM +L  KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+  AE++
Sbjct:  135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYTREGIVWQFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILNPAGVSKESDPKKCGQIILDASGLDSELYRLGHTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916          

HSP 2 Score: 352.829 bits (904), Expect = 3.003e-94
Identity = 166/259 (64.09%), Postives = 199/259 (76.83%), Query Frame = 0

Query:    9 DGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            +G DPDP+P+L VS E K   ++KPYD KKSCWVPD   G +  G I  T GD VTV  L   ++K FKKDQV QVNPPK++  +DMS LTYLNDA VL N   RY  +LIYTYSGLFC+AINPYKRFP+YT R   LY GKRR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+
Sbjct:    8 EGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264          

BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022930|ref|XP_023023703.1| (myosin heavy chain, muscle isoform X16 [Leptinotarsa decemlineata])

HSP 1 Score: 1895.94 bits (4910), Expect = 0.000e+0
Identity = 991/1789 (55.39%), Postives = 1337/1789 (74.73%), Query Frame = 0

Query:  250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
            ++ N+  KL     RR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+  YLLEK+R+  Q  LER YH FY +MS AV  +K KCLLS+NI DY++VSQGK ++  +DD E+M+   +AF ILGF +EE  NVYK+T+ VMHMG M   F   G+EEQAE    +   +V ++ G+D+  +   F KP++KVG E+VT+G+  +Q S SV  +++ +++ VF+F+  KCNETL D   K+  +IG LDIAGFEIFDYNGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGI W  +DFGMDL  CI++ EKPMG+L+ILEEES+FPKATD++F  KL+ N L K PNFQK  P P P    AHF + HYA  V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ   A +   GG R KG   +TVSS Y+ QL++LM TL +T P F+RC++PN  K+PG ++S LVMHQ  CNGVL GI ICRKGFPN+M Y DFK RY IL+A+A+      + AA+ +L+ + L+ E++RLG TKVFFRAG+LG MEE+R++R+G++++W+QS  RG  SR  FKK+Q+Q+LAL  CQR +R Y   +TW W++LW  ++P L  T+     A+ EEK A A++   +    +K++E ++  LL +K+EL   L+    ++ ++ ++  +++    D++ QL E  +R++ E+  R+ L QQ+ K++ + S  +++I  +E  L  +EQDK  KD QIR L +E+ HQ E+I KL +EK+   +N QK  E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK  KQ KELQ+R+EEL+EE+  ER +RAKAEK R+ L ++LE+LG RLEEAG  T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN  K KAE+D+ N+  +LQ+ R ++E   R KA +++  K +   + D   KLDE  R LN+ ++ KK+L +E  DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL  +L++ RE++E E E K+D  + LSKA AE QLW+ ++E+EG+ R EELE  + KLQAR+ EAEET+E+LN K+V+ EK++ RL  ++E++ LE +R +A A  +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE  EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT    E+E R R E +E +GI++R+ NAL  E+EESR LL  ++R++RQ + EL ++   +N++ + N      KR  E+ +  + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL   Q R +++ K  +++ER +KEL FQ EEDRKN ERM +L  KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+  AE++
Sbjct:  135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYTREGIVWQFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILSASAIRDNMTPEKAAQVILDHINLDPEQYRLGKTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916          

HSP 2 Score: 353.214 bits (905), Expect = 2.443e-94
Identity = 166/261 (63.60%), Postives = 200/261 (76.63%), Query Frame = 0

Query:    7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            + +G DPDP+P+L VS E K   ++KPYD KKSCWVPD   G +  G I  T GD VTV  L   ++K FKKDQV QVNPPK++  +DMS LTYLNDA VL N   RY  +LIYTYSGLFC+AINPYKRFP+YT R   LY GKRR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+
Sbjct:    6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264          

BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022932|ref|XP_023023704.1| (myosin heavy chain, muscle isoform X17 [Leptinotarsa decemlineata])

HSP 1 Score: 1892.09 bits (4900), Expect = 0.000e+0
Identity = 996/1789 (55.67%), Postives = 1341/1789 (74.96%), Query Frame = 0

Query:  250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
            ++ N+  KL     RR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+  YLLEK+R+  Q  LER YH FY +MS AV  +K KCLLS+NI DY++VSQGK ++  +DD E+M+   +AF ILGF +EE  NVYK+T+ VMHMG M   F   G+EEQAE    +   +V ++ G+D+  +   F KP++KVG E+VT+G+  +Q S SV  +++ +++ VF+F+  KCNETL D   K+  +IG LDIAGFEIFD+NGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGIEWA +DFGMDL  CI++ EKPMG+L+ILEEES+FPKATD++F  KL+ N L K PNFQK  P P P    AHF + HYA  V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ   A +   GG R KG   +TVSS Y+ QL++LM TL +T P F+RC++PN  K+PG ++S LVMHQ  CNGVL GI ICRKGFPN+M Y DFK RY ILA A +AK K+ K+AA+  L  + L+A+ +R+GHTKVFFRAG+LG MEE+R++R+G++++W+QS  RG  SR  FKK+Q+Q+LAL  CQR +R Y   +TW W++LW  ++P L  T+     A+ EEK A A++   +    +K++E ++  LL +K+EL   L+    ++ ++ ++  +++    D++ QL E  +R++ E+  R+ L QQ+ K++ + S  +++I  +E  L  +EQDK  KD QIR L +E+ HQ E+I KL +EK+   +N QK  E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK  KQ KELQ+R+EEL+EE+  ER +RAKAEK R+ L ++LE+LG RLEEAG  T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN  K KAE+D+ N+  +LQ+ R ++E   R KA +++  K +   + D   KLDE  R LN+ ++ KK+L +E  DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL  +L++ RE++E E E K+D  + LSKA AE QLW+ ++E+EG+ R EELE  + KLQAR+ EAEET+E+LN K+V+ EK++ RL  ++E++ LE +R +A A  +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE  EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT    E+E R R E +E +GI++R+ NAL  E+EESR LL  ++R++RQ + EL ++   +N++ + N      KR  E+ +  + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL   Q R +++ K  +++ER +KEL FQ EEDRKN ERM +L  KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+  AE++
Sbjct:  135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWAFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYMILAPATMAKEKEAKEAARKCLEEIGLDADSYRVGHTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916          

HSP 2 Score: 347.821 bits (891), Expect = 9.270e-93
Identity = 163/261 (62.45%), Postives = 200/261 (76.63%), Query Frame = 0

Query:    7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            + +G DPDP+P+L VS E K   ++KPYD KKSCWVPD   G +  G I  T GD VTV  L   ++K FKK+ V QVNPPK++  +DM+ LTYLNDA VL N   RY ++LIYTYSGLFC+AINPYKRFP+YT R   LY GKRR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+
Sbjct:    6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKHFKKENVHQVNPPKYEKVEDMADLTYLNDASVLHNLKQRYYSKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264          

BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022920|ref|XP_023023698.1| (myosin heavy chain, muscle isoform X11 [Leptinotarsa decemlineata])

HSP 1 Score: 1890.93 bits (4897), Expect = 0.000e+0
Identity = 996/1789 (55.67%), Postives = 1342/1789 (75.01%), Query Frame = 0

Query:  250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
            ++ N+  KL     RR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+  YLLEK+R+  Q  LER YH FY +MS AV  +K+KCLLS++IYDY +VSQGKV++ ++DD E+     +AF ILGF +EE  NVYK+T+ VMHMG M   F   G+EEQAE    +   +V ++ G+D+  +   F KP++KVG E+VT+G+  +Q S SV  +++ +++ VF+F+  KCNETL D   K+  +IG LDIAGFEIFD+NGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGIEWA +DFGMDL  CI++ EKPMG+L+ILEEES+FPKATD++F  KL+ N L K PNFQK  P P P    AHF + HYA  V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ   A +   GG R KG   +TVSS Y+ QL++LM TL +T P F+RC++PN  K+PG ++S LVMHQ  CNGVL GI ICRKGFPN+M Y DFK RY ILA A +AK K+ K+AA+  L  + L+A+ +R+GHTKVFFRAG+LG MEE+R++R+G++++W+QS  RG  SR  FKK+Q+Q+LAL  CQR +R Y   +TW W++LW  ++P L  T+     A+ EEK A A++   +    +K++E ++  LL +K+EL   L+    ++ ++ ++  +++    D++ QL E  +R++ E+  R+ L QQ+ K++ + S  +++I  +E  L  +EQDK  KD QIR L +E+ HQ E+I KL +EK+   +N QK  E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK  KQ KELQ+R+EEL+EE+  ER +RAKAEK R+ L ++LE+LG RLEEAG  T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN  K KAE+D+ N+  +LQ+ R ++E   R KA +++  K +   + D   KLDE  R LN+ ++ KK+L +E  DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL  +L++ RE++E E E K+D  + LSKA AE QLW+ ++E+EG+ R EELE  + KLQAR+ EAEET+E+LN K+V+ EK++ RL  ++E++ LE +R +A A  +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE  EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT    E+E R R E +E +GI++R+ NAL  E+EESR LL  ++R++RQ + EL ++   +N++ + N      KR  E+ +  + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL   Q R +++ K  +++ER +KEL FQ EEDRKN ERM +L  KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+  AE++
Sbjct:  135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKEKCLLSNDIYDYNYVSQGKVTIPNVDDGEECALTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWAFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYMILAPATMAKEKEAKEAARKCLEEIGLDADSYRVGHTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916          

HSP 2 Score: 353.214 bits (905), Expect = 2.184e-94
Identity = 166/261 (63.60%), Postives = 200/261 (76.63%), Query Frame = 0

Query:    7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            + +G DPDP+P+L VS E K   ++KPYD KKSCWVPD   G +  G I  T GD VTV  L   ++K FKKDQV QVNPPK++  +DMS LTYLNDA VL N   RY  +LIYTYSGLFC+AINPYKRFP+YT R   LY GKRR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+
Sbjct:    6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264          

BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022924|ref|XP_023023700.1| (myosin heavy chain, muscle isoform X13 [Leptinotarsa decemlineata])

HSP 1 Score: 1887.46 bits (4888), Expect = 0.000e+0
Identity = 988/1789 (55.23%), Postives = 1334/1789 (74.57%), Query Frame = 0

Query:  250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
            ++ N+  KL     RR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+  YLLEK+R+  Q  LER YH FY +MS AV  +K KCLLS+NI DY++VSQGK ++  +DD E+M+   +AF ILGF +EE  NVYK+T+ VMHMG M   F   G+EEQAE    +   +V ++ G+D+  +   F KP++KVG E+VT+G+  +Q S SV  +++ +++ VF+F+  KCNETL D   K+  +IG LDIAGFEIFDYNGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGI W  +DFGMDL  CI++ EKPMG+L+ILEEES+FPKATD++F  KL+ N L K PNFQK  P P P    AHF + HYA  V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ   A +   GG R KG   +TVSS Y+ QL++LM TL +T P F+RC++PN  K+PG ++S LVMHQ  CNGVL GI ICRKGFPN+M Y DFK RY IL+A+A+      + AA+ +L+ + L+ E++RLG TKVFFRAG+LG MEE+R++R+G++++W+QS  RG  SR  FKK+Q+Q+LAL  CQR +R Y   +TW W++LW  ++P L  T+     A+ EEK A A++   +    +K++E ++  LL +K+EL   L+    ++ ++ ++  +++    D++ QL E  +R++ E+  R+ L QQ+ K++ + S  +++I  +E  L  +EQDK  KD QIR L +E+ HQ E+I KL +EK+   +N QK  E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK  KQ KELQ+R+EEL+EE+  ER +RAKAEK R+ L ++LE+LG RLEEAG  T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN  K K+EK+K     +L + R+S++     KA +++  K +   + D   KLDE  R LN+ ++ KK+L +E  DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL  +L++ RE++E E E K+D  + LSKA AE QLW+ ++E+EG+ R EELE  + KLQAR+ EAEET+E+LN K+V+ EK++ RL  ++E++ LE +R +A A  +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE  EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT    E+E R R E +E +GI++R+ NAL  E+EESR LL  ++R++RQ + EL ++   +N++ + N      KR  E+ +  + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL   Q R +++ K  +++ER +KEL FQ EEDRKN ERM +L  KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+  AE++
Sbjct:  135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYTREGIVWQFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILSASAIRDNMTPEKAAQVILDHINLDPEQYRLGKTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKSEKEKAQYFGELNDLRASVDHLANEKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916          

HSP 2 Score: 353.214 bits (905), Expect = 2.057e-94
Identity = 166/259 (64.09%), Postives = 199/259 (76.83%), Query Frame = 0

Query:    9 DGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
            +G DPDP+P+L VS E K   ++KPYD KKSCWVPD   G +  G I  T GD VTV  L   ++K FKKDQV QVNPPK++  +DMS LTYLNDA VL N   RY  +LIYTYSGLFC+AINPYKRFP+YT R   LY GKRR+E PPHIF +++G+Y  M+   +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+   ++    +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F   GKL+GAD+
Sbjct:    8 EGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264