myosin heavy muscle isoform x20, snap_masked-scaffold286_size222086-processed-gene-1.7 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000012578 (supercontig:LSalAtl2s:LSalAtl2s938:153000:170372:1 gene:EMLSAG00000012578 transcript:EMLSAT00000012578 description:"maker-LSalAtl2s938-augustus-gene-0.10") HSP 1 Score: 275.018 bits (702), Expect = 4.715e-74 Identity = 199/674 (29.53%), Postives = 335/674 (49.70%), Query Frame = 0
Query: 269 PPHIFGV-AEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKC-LLSDNIYDYWWVSQGK-VSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMT---KDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCK---PKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPT--MKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMF-EKPMGLLAILEEESLFPKATDQSFAAKLHEN-LLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSN----NLLIECFRDHPGQPLEAKKDAGGGG--RKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDF--------KARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVF 915
PPH+F + A + + KN +IL+ GE+G+GKTE K ++ Y ++V S + E +I++ + +LE++G+A+T+RN+NSSRFGKFI+++F + G +SGA YLLEKSR+ ER +H FY +++ + K+K L++ + Y++++QG+ V+ +DK D + +F++LGF+ EE ++K+ + ++H+GN+ K F E E+ S A + + S +I P +G E + QA +++ +Y +F +I + N+ + + + + I LD+ GFE + N FEQ+CINF NE L N+ +F EQ EY +E I+W +++ +D +++ +KP+G+ +L++ES FPKA D SF K H N L++ + + S + F V HYA V YN+ +L KN+D N ++ L + N+ F+ P L ++G K TVS+ + L L+ + + P ++ C+ PN K P + LV+ Q + N VL I I + G+P +M Y F ARY + + A V + + + G TKVF + + +E R + LQ RG +R F
Sbjct: 136 PPHLFAIGASALARQVAYPKKNVTILLNGEAGSGKTECCKLLLQYLSAVNKSASNLKTE-----QIIEASHLLESFGHAQTLRNNNSSRFGKFIQMYF-KDGIISGAKFNDYLLEKSRIVSHNSDERNFHVFYEMLAGLSREQKEKFGLMTPD--KYFYLNQGESAGVDGKNDKADFENLISSFQVLGFSLEERDAIFKVLASILHLGNVYFHRKHFR--NGVEGVEMGSNVEIKWTAHLLQLTSNSLIQVLTSRISPD-SLG-EPIIVPMNIDQALDVRDALSKSLYGTLFTWIIKRLNKIISTKSKGVGMNKGICILDMFGFEDLNENSFEQLCINFANENLHSLINKRIFKAEQAEYAKEQIDWTPINY-IDNGPILNILSKKPVGIFHLLDDESNFPKANDTSFLDKCHYNHALNELYSRPRMSSR-----EFGVKHYAGQVWYNVDGFLRKNRDSKNPEIISLLSTTRDRHLHNMFSNLFQSLPKGELLPNVNSGNDHLVTMKPRTATVSARFIENLHQLLGIIQDSHPFYVLCIKPNNSKVPAKFDMPLVLDQLRVNTVLETIMIRKTGYPIRMKYKHFVEKFKCLLGARYPNIGYYGGGTPTTKEMAXNIVEKXARXRGDDYEFGSTKVFLKEHLRKKIETERRLIHDVTVVKLQRAVRGHLARKEF 791 HSP 2 Score: 127.102 bits (318), Expect = 4.327e-29 Identity = 67/175 (38.29%), Postives = 109/175 (62.29%), Query Frame = 0
Query: 93 DDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFP-----IYTQRSIDLYIGKRRSECPPHIFGV-AEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGA 261
+DM L +N+A +LWN +RY N IYT+ G I++NPY+ F +Y +S+ Y G+ PPH+F + A + + KN +IL+ GE+G+GKTE K ++ Y ++V S + E +I++ + +LE++G+A+T+RN+NSSRFGKFI+++F + G +SGA
Sbjct: 68 EDMISLRDINEASILWNIRLRYDNRNIYTFIGTILISVNPYRAFDEDGENLYGLKSVAKYDGQILGTLPPHLFAIGASALARQVAYPKKNVTILLNGEAGSGKTECCKLLLQYLSAVNKSASNLKTE-----QIIEASHLLESFGHAQTLRNNNSSRFGKFIQMYF-KDGIISGA 236
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000004035 (supercontig:LSalAtl2s:LSalAtl2s215:559787:562711:1 gene:EMLSAG00000004035 transcript:EMLSAT00000004035 description:"augustus_masked-LSalAtl2s215-processed-gene-5.1") HSP 1 Score: 220.705 bits (561), Expect = 9.420e-59 Identity = 208/803 (25.90%), Postives = 405/803 (50.44%), Query Frame = 0
Query: 1239 ERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEI-------EASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASL-EAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALA---GEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
E+ R + E+ + L+ + L RL +A Q+++N+KRE E+ +++ +EE + E + L+ +H M EL EQID++ +K K K+K ++ +++ + ++ K + + + ++ + + N K+D M + L S++ L++ + R++ E + A+ K + +QL D + D +R ++ ++ L T+L+S I+ E + D K L ++E WK ++E E R+E+++ + K ++I + ++ ++A+ +K+ E++++RLQ++++ + + IS+ ++F + + K +DLA ++ E ++ E + EL RL + V+ ++ L DE R L +L R +H +++ R++ ++EEL A +EA+ + EN++ + EL ++R + + + KE+EFN+ +K ++SLS L E E R K E ++KKK+ I ELE++L+ AN++N Q K ++ + Y+ + ++ G A +A E+ ++ + E++ R +A+++E +NE++ +N + K E + + ++ D+ + K ++++A +A DA LR E D K+ALE ++ L ++ + E A R + +LE+RI ELE + E+A + ER VKEL Q EEDRKN + E +L KIK YK Q+EE E I+ N+ + +K Q+E E++ R++ AE S
Sbjct: 73 EQHLRRRVEREKQDLQMQILSLTERLTQAEGGAECQIDINRKREAEMSQLRKLLEELHTESEHNIHELKKRHQTAMMELQEQIDSVARSKDKVSKEKSKLQVEIESLLAQMDVFSSEKTTVRKTIERLEIQVNEYNCKIDVMCKELXAVTSERNSLKLSNDESNRRLNELKMAIENAGLDKNKVASQLRDLQIQLDNASRGKNQAESRIVALETQLKSISVEIQEYKETRIDLEKQLGHFKSEGLDWKKKYENEARLRIEDIDCLKKKFGSQIADLQDKLDAVLSKLKGMEQAKNRLQIEVQTLIKDLG-------ISQSTVKDFQSRLNISEKKCEDLAIKLREMTNMYEKADNEVKARGQELMRLSNDMDRLKMDNGVLLKDRSKLDDECRMLKSELDALKRQLHASEQENRKMAHDREELAKAFKEADVGRNRAENRIAELEAELKRLRGDSQKGLLMKEDEFNSMKKKLVSEIESLSTRLHETENRLKNEVEKMKKKMAXTITELEMSLNSANQSNVSLQNVNKTQVNKIMELTSLYDSVCK---KLDLTAGDASSLCKKMALTESELHSMKSAYGNVEKAHRVTEAKVQELNVKINELTQVNISINNTKVKLEKELGTVTSDYTDIARELKLADDRANKASHDAKHFESLLRDESAKLVRIDHAKQALECEVKSLTIKMEEIETTAITTSRRTIKQLEIRIEELESMFSKEKVCHVETATMLHKKERVVKELLCQSEEDRKNILILQESLDRLNDKIKMYKHQLEEQECISNSNIMRVKKFQRELEDASGRAEEAESS 865
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000005286 (supercontig:LSalAtl2s:LSalAtl2s282:243643:310504:-1 gene:EMLSAG00000005286 transcript:EMLSAT00000005286 description:"maker-LSalAtl2s282-snap-gene-3.34") HSP 1 Score: 157.918 bits (398), Expect = 1.707e-38 Identity = 162/719 (22.53%), Postives = 304/719 (42.28%), Query Frame = 0
Query: 267 ECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQY--AHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMI-NYF-------CKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNG------FEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIE--WANVDFGMDLQKCIDMFEKP---------------MGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKAS----PKPDPHAHFAVVHYAAT--VSYNLTSWLEKNKDPLNDTVVELFKNGSNNL-------LIECFRDH--PGQPLEAKKDAG------------------GGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPN-------------------------THKKPGGVESG---------LVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNIL---AAAAVAKAKKDKD----AAKAVLNVVKLEAEKFRLGHTKVF 878
+ PPHI+ VA+ SY+ M++ K+QSI+ G SG+GKT N K ++SYF SG + + +K+ +LEA+G+++T N N+SRF + I + F+ G L A + + EK+R+ + + E ++ FY+L+ + ++ +L + +V+Q ++ DDK + + A ++LG + E ++++ + + + H+G + +GK A ++ K A+ G SE + N F + K + + + S+ G +Y+ VF + N + DP + LD GF+ G FE++C N+ E+LQ F++ + E+Y++E +E ++D + + + +K GLL +L+EE+LFP A+D++F +L LL Q+ S + F + H+ T V YN WL +++ V L K N L + E + PG + AG GG +++ K LD +++ L T F++C +P +E+ LV Q + +L + + + G+P + Y F R+++L A + + ++ D A + + + L++ +RLG+++ +
Sbjct: 525 DMPPHIYSVAQLSYRSMLSTRKDQSIVFIGRSGSGKTTNVKHMLSYFTKT--SGNK---QVITTEKVNAIYVLLEAFGHSRTNLNSNASRFTELISLDFDASGGLISASIQAMMXEKTRVVRRPDGEPNFNIFYHLLMGSDSSTRKDLMLDNLNEPNLFVTQ----LQKPDDKSKVIWNKIQSAIKVLGISSHEAHSLWSVLAAIFHLGTASVTRGTMGKSLFARPQA---GQKAAQCLGTSSEELARNIFQGTSSTSIRSKSRDSSFRSSDLTNLPDGIDSLHGFVIGLYQEVFNTLIYMINRCISDPIANTFNSVYILDTPGFQNPASCGRIKGACFEELCHNYVQERLQLLFHKRNISSQYEKYLQEQVEPDVGDLDDIGSPESMVSLLDKQPGTRSSQTDLSSTERRGLLWLLDEEALFPGASDETFVERL---LLQYN---QRGSDELLKRGLGEKQFVLQHFQGTNPVLYNAEGWLSSSRED-----VALLKAAGNILTESNTKSISELYSSSRGPGSTSVSGSVAGLEGSISLRRASSIRRAFTTGGHAGIKRHSIALQQKFLLDGVLEQLRRTKVHFVQCFLPQHGAGLCDIVGRSASKGSNSSTSSSIGSGDSLSLEANNSNVLMNIPLVRSQVRGGQILDSVRLFKNGYPESIPYALFWRRFHVLGENANQGLTEPPRNVDHLKTAVSEIFSFLDLDSSAYRLGNSQRY 1220 HSP 2 Score: 136.732 bits (343), Expect = 4.406e-32 Identity = 73/203 (35.96%), Postives = 118/203 (58.13%), Query Frame = 0
Query: 62 KVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMR 264
KV + + D ++D V + NPP +D +D++ L YLN++ VL RY N LI+TY+G I+INP IY+ + I ++ + + PPHI+ VA+ SY+ M++ K+QSI+ G SG+GKT N K ++SYF SG + + +K+ +LEA+G+++T N N+SRF + I + F+ G L A ++
Sbjct: 433 KVRIQLDYNGDVLEVEEDDVEKANPPGYDKVEDLAQLRYLNESSVLNTLRQRYGNNLIHTYAGPSLISINPMSPLAIYSDKVIQMFKECKTEDMPPHIYSVAQLSYRSMLSTRKDQSIVFIGRSGSGKTTNVKHMLSYFTKT--SGNK---QVITTEKVNAIYVLLEAFGHSRTNLNSNASRFTELISLDFDASGGLISASIQ 630
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000002471 (supercontig:LSalAtl2s:LSalAtl2s147:318144:322176:1 gene:EMLSAG00000002471 transcript:EMLSAT00000002471 description:"maker-LSalAtl2s147-augustus-gene-3.7") HSP 1 Score: 136.732 bits (343), Expect = 5.789e-37 Identity = 66/140 (47.14%), Postives = 92/140 (65.71%), Query Frame = 0
Query: 37 KKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKN 176
K+ WVP S G+ + D+V V I+E+ + KD + ++NPPKFD +DM+ LT LN+A VL N RY + LIYTYSGLFC+ +NPYKR PIYT++ I+LY GK+R E PPH+F + + +Y+ M+ GKN
Sbjct: 33 KRLVWVPHD-SHGFVAASXVTNMKDEVEVEIVESGKRIEVSKDDIQKMNPPKFDKEEDMAXLTCLNEASVLHNVKERYYSGLIYTYSGLFCVVVNPYKRLPIYTEKIIELYKGKKRHEVPPHVFAITDTAYRSMLQ-GKN 170
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Match: EMLSAG00000008139 (supercontig:LSalAtl2s:LSalAtl2s485:3423:47423:-1 gene:EMLSAG00000008139 transcript:EMLSAT00000008139 description:"maker-LSalAtl2s485-augustus-gene-0.6") HSP 1 Score: 134.035 bits (336), Expect = 1.920e-31 Identity = 138/576 (23.96%), Postives = 236/576 (40.97%), Query Frame = 0
Query: 339 VLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSD-NIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNE----------TLFDPTMKKVQ------------------------------------YIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEK-PMGLLAILEEESLFPKATDQSFAAKL---HENLLSKCPNFQKASPKP-DPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHP-GQPLEAKKDAGGGGRKKG----------------GGKTVSSF---YKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILA 842
VL + G AKT N SSR G FI + + G L + Y L++SR+ +E+ YH FY +++ D ++ L I D +++ G + D E + ILG +V ++ + ++ +GN+ DF GK + E+ + VA + G+ S + V + V + A+++ +A+ +Y I + N T+ + + VQ +IG LD+ GFE + E +CIN C+E +Q F+N H+F E EGI V +D CID+ GLL++L+ E + +++ K+ H++ N + PK D FA+ HYA V Y+ + +L+ N+D + D ++ +F + F H G ++A + R G + VS+ + +LD+L++TL P F+RC+ PN + + LV Q + +L + + GFP++M + F RY LA
Sbjct: 390 VLRSLGMAKTASNRESSRIGHFIEVQVSDGA-LYRTKIHCYFLDQSRVVQPLPMEKNYHIFYQMLAGLSQDERRSLGLDGLTIRDLNFLNSGDYRQDEAQDAERFEEWKVNLGILGI---PFMDVVRVLASILLLGNI--DFAE-GKGLEVEVVGREELNSVASLLGVPSSHLWQGLTTRTYSVRGQLVKSMSDINMAAATRNALAKALYCRTVATIVRRANSLKRPVGAMSGTMSSDSNESVQHDVASHQASTVGTAGSRKSSKSMAILNHAVRHATDGFIGILDMFGFEDSKPSRLEHLCINLCSETMQHFYNTHIFKSSIESCRDEGIITDLVVDYVDNVPCIDLISSLRTGLLSMLDVECSV-RGCPETYVQKVKIQHKD------NKKLXEPKHCDISRTFAIHHYAGQVVYDTSYFLDTNRDVIPDDLLCVFDRDTCQF---GFATHLFGNEIKALRSQDTIPRGVSFRISPTSSSTSPELLNGDEPVSTLTQDFHTRLDNLLRTLVHAKPHFVRCIKPNNKESSTEFDRTLVAQQIRSLQILETVNLMSGGFPHRMRFKAFNTRYRCLA 948
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|110825729|sp|P05661.4|MYSA_DROME (RecName: Full=Myosin heavy chain, muscle) HSP 1 Score: 1805.8 bits (4676), Expect = 0.000e+0 Identity = 973/1779 (54.69%), Postives = 1297/1779 (72.91%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEG---EASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPH--AHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGK-------TVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
RR+E PPHIF +++G+Y M+ NQS+LITGESGAGKTENTKKVI+YFA+VGAS K E + SLED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS +VP +K CLL+DNIYDY VSQGKV+V SIDD E+ AF ILGF ++E +VY++T+ VMHMG M F G+EEQAE E+ +V+++ G D+ + KP++KVG E+VT+G+ Q ++S+ + + +++ +F+++ KCNETL D K+ +IG LDIAGFEIF+YNGFEQ+CINF NEKLQQFFN MFV+EQEEY +EGI W +DFGMDL CID+ EKPMG+L+ILEEES+FPKATDQ+F+ KL L K FQK P AHFA+ HYA VSYN+T WLEKNKDPLNDTVV+ FK N LLIE F DH GQ +GGG + KGG TVSS YK QL+ LM TL +T P F+RC++PN K+PG V++ LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY IL + K A+K ++ +L + +RLGHTKVFFRAG+LG MEE R++R+G+++SW+Q+ ARG SR FKK+Q+Q++AL QR +R Y +TW W++LW +KP L ++ A EEK AE+ + RK++E ++ LL +K+ L +L A+QD ++N ++ D++ QL ++ R+ E+ R+ L QQ+ K + S L+++I +E + AEQDK KD QIR L +E+ HQ E+I KL +EK+ + QKT E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKKV+ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE + K+ +Q KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HE TLA LR KHN+ ++E+ EQ+D LN K KAE D+ +L + R++ + R KA ++ K +Q ++ + SKLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K KISLTTQLEDTKRLAD E+R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE Q+W+S++E++G+ R EELE + KLQAR+ EAEET+E+LN K + EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLR+QLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+D E+E R R + +E +GI++R+ NAL E+EESR LL ++R +RQ + EL ++ +NE+S+ N KR ES + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALE Q+ EL+ RL +AEANA K G+ A+ KLE R+RELE EL Q R +++ K +++ER VKEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 147 RRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGSVPGVKDICLLTDNIYDYHIVSQGKVTVASIDDAEEFSLTDQAFDILGFTKQEKEDVYRITAAVMHMGGMK--FKQRGREEQAEQDGEEEGGRVSKLFGCDTAELYKNLLKPRIKVGNEFVTQGRNVQQVTNSIGALCKGVFDRLFKWLVKKCNETL-DTQQKRQHFIGVLDIAGFEIFEYNGFEQLCINFTNEKLQQFFNHIMFVMEQEEYKKEGINWDFIDFGMDLLACIDLIEKPMGILSILEEESMFPKATDQTFSEKLTNTHLGKSAPFQKPKPPKPGQQAAHFAIAHYAGCVSYNITGWLEKNKDPLNDTVVDQFKKSQNKLLIEIFADHAGQ-------SGGGEQAKGGRGKKGGGFATVSSAYKEQLNSLMTTLRSTQPHFVRCIIPNEMKQPGVVDAHLVMHQLTCNGVLEGIRICRKGFPNRMMYPDFKMRYQILNPRGIKDLDCPKKASKVLIESTELNEDLYRLGHTKVFFRAGVLGQMEEFRDERLGKIMSWMQAWARGYLSRKGFKKLQEQRVALKVVQRNLRKYLQLRTWPWYKLWQKVKPLLNVSRIEDEIARLEEKAKKAEELHAAEVKVRKELEALNAKLLAEKTALLDSLSGEKGALQDYQERNAKLTAQKNDLENQLRDIQERLTQEEDARNQLFQQKKKADQEISGLKKDIEDLELNVQKAEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKMQGETNQKTGEELQAAEDKINHLNKVKAKLEQTLDELEDSLEREKKVRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKELSSITAKLEDEQVVVLKHQRQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELSKLRRDLEEANIQHESTLANLRKKHNDAVAEMAEQVDQLNKLKAKAEHDRQTCHNELNQTRTACDQLGRDKAAQEKIAKQLQHTLNEVQSKLDETNRTLNDFDASKKKLSIENSDLLRQLEEAESQVSQLSKIKISLTTQLEDTKRLADEESRERATLLGKFRNLEHDLDNLREQVEEEAEGKADLQRQLSKANAEAQVWRSKYESDGVARSEELEEAKRKLQARLAEAEETIESLNQKCIGLEKTKQRLSTEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRAQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKNIKRYQQQLKDIQTALEEEQRARDDAREQLGISERRANALQNELEESRTLLEQADRGRRQAEQELADAHEQLNEVSAQNASISAAKRKLESELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALEQQIKELQVRLDEAEANALKGGKKAIQKLEQRVRELENELDGEQRRHADAQKNLRKSERRVKELSFQSEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1915 HSP 2 Score: 330.487 bits (846), Expect = 7.668e-90 Identity = 156/266 (58.65%), Postives = 197/266 (74.06%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEG---EASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MP + ++ D DP+P+L VS E + +SKPYD KKSCW+PD+ G Y G I +T GD V+V L+ + + K ++V +VNPPKF+ +DM+ +T LN CVL N RY +LIYTYSGLFC+AINPYKR+P+YT R +Y GKRR+E PPHIF +++G+Y M+ NQS+LITGESGAGKTENTKKVI+YFA+VGAS K E + SLED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 1 MPKPV--ANQEDEDPTPYLFVSLEQRRIDQSKPYDSKKSCWIPDEKEG-YLLGEIKATKGDIVSVG-LQGGEVRDIKSEKVEKVNPPKFEKIEDMADMTVLNTPCVLHNLRQRYYAKLIYTYSGLFCVAINPYKRYPVYTNRCAKMYRGKRRNEVPPHIFAISDGAYVDMLTNHVNQSMLITGESGAGKTENTKKVIAYFATVGASKKTDEAAKSKGSLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 262
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|127773|sp|P24733.1|MYS_ARGIR (RecName: Full=Myosin heavy chain, striated muscle) HSP 1 Score: 1595.1 bits (4129), Expect = 0.000e+0 Identity = 848/1777 (47.72%), Postives = 1229/1777 (69.16%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEAS------LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPH---AHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKD-KDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
R++E PPH+F VA+ +YQ M+ +NQS LITGESGAGKTENTKKVI Y A V + K+K+ EAS LED+I+Q NPVLEA+GNAKT RN+NSSRFGKFIRI F GK++GAD+ YLLEKSR+T+Q ER YH FY + S+A+P+L L++ + Y +++QG ++V++IDD E+ + +AF ILGF +EE +++K T+ ++HMG M F +EEQAE A KVA +CGI++ ++ KPK+KVGTE VTKGQ +Q +SV +A+ +Y+ +F ++ + N+TL D K+ YIG LDIAGFEIFD+N FEQ+CIN+ NE+LQQFFN HMF+LEQEEY +EGI W +DFGMDLQ CID+ EKPMG+L+ILEEE +FPKA D+SF KL++N + K F K P+ AHF + HYA V Y++T WLEKNKDP+N+ VV L L+ E F+ P E K +T+S+ ++ L+ LMK LY+T P F+RC++PN K+PG V++ LV+HQ QCNGVL GI ICRKGFP+++ Y +FK RY+ILA A+ + D K ++ +L ++++ ++RLG TKVFF+AG+LG +EE+R++R+ +++S Q+ RG R +KK+QDQ++ L QR IR + + + W WW+L+ +KP L + + E +++ ++++ K +K++E + LL QK++L L L++ ++ D ++ ++ AD + Q+ E+ R+ E+ L + K+++ + L+++I +E L AEQDK KD QI TL+ E+ Q E I KL +EK+ +++ +KT + +QA EDK +HL+K+K KLEQ+LDE ED LE EKKV+ D EK KRK+E DLK TQE + DLERVK EL + V+RKE E +++N+K++DE L + ++ KELQ+R+EEL+EEL ER +RAK EK R+ L ++LE+LG RL+EAG T+ Q+ELNKKRE EL +I+ ++EE ++ HE ++ALR KH + +E+ +Q+D L K K EKDK +++R++ + S + ++ K ++ K + + D N++L++ R++NE +SQK RL+ E DL RQ+E+ E+ ++ +K+K L++QLED +R + E R RS L + +N+ ++++ RE++E E E KSD + LSKA EIQ W+S+FE+EG R EELE + KL ++ EAE+T EA N K + EK++SRLQ +LE+MS+E +R +A+ EK+ R FD+ EW+AK + L E+E S KE R Y++EL+R++A+ EE + + ++RENKNLADEI DL DQL +GGRS HELDK RRRLE+EKEELQAALEEAE ALEQEE KV+R+QLE+ VR EID++IQEKEEEF+NTR+NHQRA++S+ ASLEAE + K +A+RIKKKLE DINELE+ALD +N+ AE +K VKRYQ Q+R+ E+E R R E +E+ +A+R+ ++GEVEE RA L +ER+++ D EL ++ VNE++S + +KR E I+A+Q ++D+M + K ++E+ K+AM DAARLADELR+EQ+HS ++ ++ LESQ+ E + RL +AEA++ K G+ + KLE R+ ELE EL + Q R +E+ K ++A+R +KEL FQ +EDRKNQER+ EL KL KIKT+K+Q+EEAEEIAA+NLAK+RK+Q E EE+E+R+ TA+ +
Sbjct: 144 RKTEIPPHLFSVADNAYQNMVTDRENQSCLITGESGAGKTENTKKVIMYLAKVACAVKKKDEEASDKKEGSLEDQIIQANPVLEAYGNAKTTRNNNSSRFGKFIRIHFGPTGKIAGADIETYLLEKSRVTYQQSAERNYHIFYQICSNAIPELNDVMLVTPDSGLYSFINQGCLTVDNIDDVEEFKLCDEAFDILGFTKEEKQSMFKCTASILHMGEMK--FKQRPREEQAESDGTAEAEKVAFLCGINAGDLLKALLKPKVKVGTEMVTKGQNMNQVVNSVGALAKSLYDRMFNWLVRRVNKTL-DTKAKRNYYIGVLDIAGFEIFDFNSFEQLCINYTNERLQQFFNHHMFILEQEEYKKEGIAWEFIDFGMDLQMCIDLIEKPMGILSILEEECMFPKADDKSFQDKLYQNHMGKNRMFTKPGKPTRPNQGPAHFELHHYAGNVPYSITGWLEKNKDPINENVVALLGASKEPLVAELFK----APEEPAGGGKKKKGKSSAFQTISAVHRESLNKLMKNLYSTHPHFVRCIIPNELKQPGLVDAELVLHQLQCNGVLEGIRICRKGFPSRLIYSEFKQRYSILAPNAIPQGFVDGKTVSEKILAGLQMDPAEYRLGTTKVFFKAGVLGNLEEMRDERLSKIISMFQAHIRGYLIRKAYKKLQDQRIGLSVIQRNIRKWLVLRNWQWWKLYSKVKPLLSIARQEEEMKEQLKQMDKMKEDLAKTERIKKELEEQNVTLLEQKNDLFLQLQTLEDSMGDQEERVEKLIMQKADFESQIKELEERLLDEEDAAADLEGIKKKMEADNANLKKDIGDLENTLQKAEQDKAHKDNQISTLQGEISQQDEHIGKLNKEKKALEEANKKTSDSLQAEEDKCNHLNKLKAKLEQALDELEDNLEREKKVRGDVEKAKRKVEQDLKSTQENVEDLERVKRELEENVRRKEAEISSLNSKLEDEQNLVSQLQRKIKELQARIEELEEELEAERNARAKVEKQRAELNRELEELGERLDEAGGATSAQIELNKKREAELLKIRRDLEEASLQHEAQISALRKKHQDAANEMADQVDQLQKVKSKLEKDKKDLKREMDDLESQMTHNMKNKGCSEKVMKQFESQMSDLNARLEDSQRSINELQSQKSRLQAENSDLTRQLEDAEHRVSVLSKEKSQLSSQLEDARRSLEEETRARSKLQNEVRNMHADMDAIREQLEEEQESKSDVQRQLSKANNEIQQWRSKFESEGANRTEELEDQKRKLLGKLSEAEQTTEAANAKCSALEKAKSRLQQELEDMSIEVDRANASVNQMEKKQRAFDKTTAEWQAKVNSLQSELENSQKESRGYSAELYRIKASIEEYQDSIGALRRENKNLADEIHDLTDQLSEGGRSTHELDKARRRLEMEKEELQAALEEAEGALEQEEAKVMRAQLEIATVRNEIDKRIQEKEEEFDNTRRNHQRALESMQASLEAEAKGKADAMRIKKKLEQDINELEVALDASNRGKAEMEKTVKRYQQQIREMQTSIEEEQRQRDEARESYNMAERRCTLMSGEVEELRAALEQAERARKASDNELADANDRVNELTSQVSSVQGQKRKLEGDINAMQTDLDEMHGELKGADERCKKAMADAARLADELRAEQDHSNQVEKVRKNLESQVKEFQIRLDEAEASSLKGGKKMIQKLESRVHELEAELDNEQRRHAETQKNMRKADRRLKELAFQADEDRKNQERLQELIDKLNAKIKTFKRQVEEAEEIAAINLAKYRKAQHELEEAEERADTADST 1913 HSP 2 Score: 302.753 bits (774), Expect = 2.750e-81 Identity = 154/312 (49.36%), Postives = 200/312 (64.10%), Query Frame = 0
Query: 9 DGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA------SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM-------------RRSECPPHIFGVAEGSYQGMMNVGK--NQSILITGESG 299
D DPD +L V + K ++ +D KK+CWVPD+ G + I S+ GD++TV I+ + KKD + +NPPKF+ +DM+ +TYLN+A VL+N RY + LIYTYSGLFCIA+NPY+R PIYT I Y GKR++E PPH+F VA+ +YQ M+ +NQS LITGESGAGKTENTKKVI Y A V + K+K+ EA SLED+I+Q NPVLEA+GNAKT RN+NSSRFGKFIRI F GK++GAD+ + +E HIF YQ N N +L+T +SG
Sbjct: 4 DFSDPD-FQYLAVDRKKLMKEQTAAFDGKKNCWVPDEKEG-FASAEIQSSKGDEITVKIVADSSTRTVKKDDIQSMNPPKFEKLEDMANMTYLNEASVLYNLRSRYTSGLIYTYSGLFCIAVNPYRRLPIYTDSVIAKYRGKRKTEIPPHLFSVADNAYQNMVTDRENQSCLITGESGAGKTENTKKVIMYLAKVACAVKKKDEEASDKKEGSLEDQIIQANPVLEAYGNAKTTRNNNSSRFGKFIRIHFGPTGKIAGADIETYLLEKSRVTYQQSAERNYHIF------YQICSNAIPELNDVMLVTPDSG 307
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|125987844|sp|P79293.2|MYH7_PIG (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1510.35 bits (3909), Expect = 0.000e+0 Identity = 807/1776 (45.44%), Postives = 1204/1776 (67.79%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPG--QPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ +AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q + +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K NFQK + K P AHFA++HYA TV YN+ WL+KNKDPLN+TVV+L+K S LL F ++ G P+E K K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG SRM FKK+ +++ +L Q IR + K W W +L+ IKP LK + + A +E+ ++ + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ E++RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ E+ + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVMYATGALAKAVYEKMFNWMVTRINTTL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGRPEAHFALIHYAGTVDYNIIGWLQKNKDPLNETVVDLYKKSSLKLLSNLFANYAGADTPVEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMSVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEMERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMEADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 276.174 bits (705), Expect = 3.474e-73 Identity = 132/255 (51.76%), Postives = 175/255 (68.63%), Query Frame = 0
Query: 14 DPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +P+L S + + + +++P+D KK +VPD + + I S +G KVT K V K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY + +IYTYSGLFC+ INPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 11 EAAPYLRKSEKERLEAQTRPFDLKKDVYVPDDKEE-FVKAKILSREGGKVTAETEHGKTVTV-KEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTINPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|81871557|sp|Q91Z83.1|MYH7_MOUSE (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1508.43 bits (3904), Expect = 0.000e+0 Identity = 807/1774 (45.49%), Postives = 1202/1774 (67.76%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q S ++ +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K NFQK + K AHF++VHYA TV YN+ WL+KNKDPLN+TVV L++ S LL F ++ G A K G +K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG SRM FKK+ +++ +L Q IR + K W W +L+ IKP LK + + A +E+ + + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G+SIHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ++ + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDSAFDVLGFTPEEKNSIYKLTGAIMHFGNMK--FKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVSYAIGALAKSVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNILGWLQKNKDPLNETVVGLYQKSSLKLLSNLFANYAGADAPADK-GKGKAKKGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIVKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSTGKSIHELEKIRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 275.018 bits (702), Expect = 7.787e-73 Identity = 132/254 (51.97%), Postives = 176/254 (69.29%), Query Frame = 0
Query: 17 PWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVF--KKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
P+L S + + + +++P+D KK +VPD + + I S +G KVT ET++ K K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY + +IYTYSGLFC+ +NPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 14 PFLRKSEKERLEAQTRPFDLKKDVFVPDDKEE-FVKAKIVSREGGKVTA---ETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|75054114|sp|Q8MJU9.1|MYH7_HORSE (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1507.27 bits (3901), Expect = 0.000e+0 Identity = 805/1776 (45.33%), Postives = 1205/1776 (67.85%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ--PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I++ NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ +AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q + + +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL +N L K NFQK + K P AHF+++HYA TV YN+ WL+KNKDPLN+TVV+L+K S +L F ++ G P+E K K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L+ + ++ ++R GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG +RM FKK+ +++ +L Q IR + K W W +L+ IKP LK + + A +E+ A ++ + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E ++K ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ++ + +Q E+++ + + +++EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTSGKGTLEDQIIEANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYAKGALAKAVYERMFNWMVARINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSSNFQKPRNIKGKPEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVDLYKKSSLKMLSNLFANYLGADAPIEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYRFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFARLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMKRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRDAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEVEQKRNAESIKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 272.707 bits (696), Expect = 3.782e-72 Identity = 130/253 (51.38%), Postives = 174/253 (68.77%), Query Frame = 0
Query: 16 SPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+P+L + + + + +++P+D KK +VPD + + I S +G K+T K V K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY +IYTYSGLFC+ INPYK P+YT + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I++ NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 13 APYLRKTEKERLEDQTRPFDLKKDVFVPDDKEE-FVKAKIISREGGKITAETEHGKTVTV-KEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYAAWMIYTYSGLFCVTINPYKWLPVYTAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTSGKGTLEDQIIEANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|83304912|sp|P12883.5|MYH7_HUMAN (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1506.89 bits (3900), Expect = 0.000e+0 Identity = 804/1776 (45.27%), Postives = 1203/1776 (67.74%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ--PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ +AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q + +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL +N L K NFQK + K P AHF+++HYA V YN+ WL+KNKDPLN+TVV L++ S LL F ++ G P+E K K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L+ + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG +RM +KK+ +++ +L Q IR + K W W +L+ IKP LK + + A +E+ ++ + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+N R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ EAEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ++ + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVIYATGALAKAVYERMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSANFQKPRNIKGKPEAHFSLIHYAGIVDYNIIGWLQKNKDPLNETVVGLYQKSSLKLLSTLFANYAGADAPIEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLARMEYKKLLERRDSLLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAEREKEMASMKEEFTRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQEAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKMELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 276.174 bits (705), Expect = 3.980e-73 Identity = 132/253 (52.17%), Postives = 175/253 (69.17%), Query Frame = 0
Query: 16 SPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+P+L S + + + +++P+D KK +VPD + + I S +G KVT K V K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY + +IYTYSGLFC+ +NPYK P+YT + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 13 APYLRKSEKERLEAQTRPFDLKKDVFVPDDKQE-FVKAKIVSREGGKVTAETEYGKTVTV-KEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKDRYGSWMIYTYSGLFCVTVNPYKWLPVYTPEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQSPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|125987843|sp|P49824.3|MYH7_CANFA (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1506.12 bits (3898), Expect = 0.000e+0 Identity = 804/1776 (45.27%), Postives = 1205/1776 (67.85%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQ--PLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ +AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q + + +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K NFQK + K AHF+++HYA TV YN+ WL+KNKDPLN+TVV L++ S LL F ++ G P+E K K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L+ + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG SRM +KK+ +++ +L Q IR + K W W +L+ IKP LK + + A +E+ A ++ + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G++IHEL+K R++LE EK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ++ + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDSEELMATDNAFDVLGFTSEEKNSMYKLTGAIMHFGNMK--FKQKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVAYATGALAKAVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLQKNKDPLNETVVALYQKSSLKLLSNLFANYAGADAPVEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEYKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFARIKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKGLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMDADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 275.789 bits (704), Expect = 5.450e-73 Identity = 132/254 (51.97%), Postives = 176/254 (69.29%), Query Frame = 0
Query: 17 PWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVF--KKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
P+L S + + + +++P+D KK +VPD + + I S +G KVT ET++ K K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY + +IYTYSGLFC+ +NPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 14 PFLRKSEKERLEAQTRPFDLKKDVFVPDDKEE-FVKAKIVSREGGKVTA---ETENGKTVTVKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKDQTPGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|75055810|sp|Q9BE39.1|MYH7_BOVIN (RecName: Full=Myosin-7; AltName: Full=Myosin heavy chain 7; AltName: Full=Myosin heavy chain slow isoform; Short=MyHC-slow; AltName: Full=Myosin heavy chain, cardiac muscle beta isoform; Short=MyHC-beta) HSP 1 Score: 1505.73 bits (3897), Expect = 0.000e+0 Identity = 809/1783 (45.37%), Postives = 1205/1783 (67.58%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPG--QPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIG-------KALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY ++SQG+ +V SIDD E++ +AF +LGF EE ++YKLT +MH GNM F +EEQAE + A K A + G++S ++ C P++KVG E+VTKGQ Q + +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL +N L K NFQK + K P AHF+++HYA TV YN+ WL+KNKDPLN+TVV+L+K S +L F ++ G P+E K K +TVS+ ++ L+ LM L +T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+Q+RG SRM FKK+ +++ +L Q IR + K W W +L+ IKP LK AE E++IA+ ++ G K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+ +I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++K+ E A+NA+I+DE LG + K+ KELQ+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++EL EQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + + SK +E R++N+ SQ+ +L+ E +L RQ++E E ++Q + K++ T QLED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG G++IHEL+K R++LE EK ELQ+ALEEAEA+LEQEE K+LR+QLE Q++ E++RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ E+ + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EEDRKN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQATGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERDYHIFYQILSNKKPELLDMLLITNNPYDYAFISQGETTVASIDDAEELMATDNAFDVLGFTTEEKNSMYKLTGAIMHFGNMK--FKLKQREEQAEPDGTEEADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQNVQQVVYAKGALAKAVYERMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLFDNHLGKSSNFQKPRNIKGKPEAHFSLIHYAGTVDYNIIGWLQKNKDPLNETVVDLYKKSSLKMLSSLFANYAGFDTPIEKGKGKAK---KGSSFQTVSALHRENLNKLMTNLRSTHPHFVRCIIPNETKSPGVIDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQSRGVLSRMEFKKLLERRDSLLIIQWNIRAFMGVKNWPWMKLYFKIKPLLKS-------AETEKEIALMKEEFGRLKEALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLADAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKRDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKAKVKLEQHVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKQQLDERLKKKDFELNALNARIEDEQALGSQLQKKLKELQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELSEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKMCRTLEDQMNEHRSKAEETQRSVNDLTSQRAKLQTENGELSRQLDEKEALISQLTRGKLTYTQQLEDLKRQLEEEVKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGSSGKTIHELEKVRKQLEAEKLELQSALEEAEASLEQEEGKILRAQLEFNQIKAEMERKLAEKDEEMEQAKRNHLRVVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSHANRLAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMEADLSQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDRKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1914 HSP 2 Score: 276.559 bits (706), Expect = 2.692e-73 Identity = 132/255 (51.76%), Postives = 175/255 (68.63%), Query Frame = 0
Query: 14 DPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKE-----GEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +P+L S + + + +++P+D KK +VPD + + I S +G KVT K V K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY + +IYTYSGLFC+ INPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFA + A G R + G+ +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 11 EAAPYLRKSEKERLEAQTRPFDLKKDVFVPDDKEE-FVKATILSREGGKVTAETEHGKTVTV-KEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYASWMIYTYSGLFCVTINPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFAVIAAIGDRSKKEQATGKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 263
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|317373582|sp|P13533.5|MYH6_HUMAN (RecName: Full=Myosin-6; AltName: Full=Myosin heavy chain 6; AltName: Full=Myosin heavy chain, cardiac muscle alpha isoform; Short=MyHC-alpha) HSP 1 Score: 1501.11 bits (3885), Expect = 0.000e+0 Identity = 806/1775 (45.41%), Postives = 1199/1775 (67.55%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKR------KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFAS+ A G R + +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY +VSQG+VSV SIDD E++ AF +LGF EE VYKLT +MH GNM F +EEQAE ++A K A + G++S ++ C P++KVG E+VTKGQ+ Q S+ +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKATD +F AKL++N L K NFQK + K AHF+++HYA TV YN+ WLEKNKDPLN+TVV L++ S L+ F + + GG +K +TVS+ ++ L+ LM L T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL A+ + + + + +L+ + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+QARG+ R+ FKK+ +++ AL Q IR + K W W +L+ IKP LK + + A +E+ ++ + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+N R+ E++ LT ++ K++ + S+L+++I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+Q EDK + LSK K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++KE + N+KI+DE L + K+ KE Q+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + KL+E R+LN+ +Q+ +L+ E +L RQ+EE E ++Q + K+S T Q+ED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG+GG+++HEL+K R++LEVEK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NHQR +DSL SL+AE R++ E LR+KKK+E D+NE+EI L AN+ AE QK VK Q L+DT +D R +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ES + +Q+E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EED+KN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHYGNMK--FKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKAVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRIKETLEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQVEEDKVNSLSKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDINQQNSKIEDEQVLALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKVRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHQRVVDSLQTSLDAETRSRNEVLRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQSEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELEGELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1916 HSP 2 Score: 269.24 bits (687), Expect = 4.383e-71 Identity = 128/242 (52.89%), Postives = 167/242 (69.01%), Query Frame = 0
Query: 30 KSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVF--KKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKR------KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+++P+D + C+VPD + + I S +G KV I ET++ K K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY +IYTYSGLFC+ +NPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFAS+ A G R + +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 27 QTRPFDIRTECFVPDDKEE-FVKAKILSREGGKV---IAETENGKTVTVKEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLFNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 264
BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Match: gi|3024204|sp|Q02566.2|MYH6_MOUSE (RecName: Full=Myosin-6; AltName: Full=Myosin heavy chain 6; AltName: Full=Myosin heavy chain, cardiac muscle alpha isoform; Short=MyHC-alpha) HSP 1 Score: 1495.33 bits (3870), Expect = 0.000e+0 Identity = 805/1775 (45.35%), Postives = 1198/1775 (67.49%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGE------ASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQY-IGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKA-SPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAK--KDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFAS+ A G R + E +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+ YLLEKSR+ FQ + ER YH FY ++S+ P+L L+++N YDY +VSQG+VSV SIDD E++ AF +L F EE VYKLT +MH GNM F +EEQAE ++A K A + G++S ++ C P++KVG E+VTKGQ+ Q S+ +A+ +YE +F ++ + N TL T + QY IG LDIAGFEIFD+N FEQ+CINF NEKLQQFFN HMFVLEQEEY +EGIEW +DFGMDLQ CID+ EKPMG+++ILEEE +FPKA+D +F AKL++N L K NFQK + K AHF++VHYA TV YN+ WLEKNKDPLN+TVV L++ S L+ F + GG +K +TVS+ ++ L+ LM L T P F+RC++PN K PG +++ LVMHQ +CNGVL GI ICRKGFPN++ Y DF+ RY IL AA+ + + + A+ +L + ++ +++ GHTKVFF+AG+LG +EE+R++R+ +++ +Q+QARG+ R+ FKK+ +++ AL Q IR + K W W +L+ IKP LK + + A +E+ + + K+ A RK++E LL +K++L L +++ + D ++ ++ ++ ++ E+ R+ E++ LT ++ K++ + S+L+++I+ +E LA E++K + +++ L EE+ E+I KL +EK+ +Q+ Q+ +D+QA EDK + L+K K KLEQ +D+ E +LE EKKV+ D E+ KRK+E DLKLTQE+I DLE K +L + +++KE + + N+KI+DE L + K+ KE Q+R+EEL+EEL ER +RAK EK RS L ++LE++ RLEEAG T+ Q+E+NKKRE E +++ ++EE + HE T AALR KH ++++ELGEQIDNL K K EK+K + +L + S++E ++AKA L++ + ++ + KL+E R+LN+ +Q+ +L+ E +L RQ+EE E ++Q + K+S T Q+ED KR + E + +++L ++ + + RE+ E E E K++ + LSKA +E+ W++++ET+ + R EELE + KL R+ +AEE VEA+N K S EK++ RLQ ++E++ ++ ER++AAA +K+ RNFD+++ EWK K ++ E+E+S KE R+ ++ELF+L+ A+EE++E L+ KRENKNL +EI DL +QLG+GG+++HEL+K R++LEVEK ELQ+ALEEAEA+LE EE K+LR+QLE Q++ EI+RK+ EK+EE ++NH R +DSL SL+AE R++ EALR+KKK+E D+NE+EI L QAN+ +E QK +K Q L+DT +D +++E + I +R+ N L E+EE RA++ +ERS++ + EL E+ V + S NT +++K+ ES + +Q E+++ + + +N+EEKAK+A+ DAA +A+EL+ EQ+ S +R K+ +E + +L+ RL +AE A K G+ + KLE R+RELE EL + Q R +ES K +++ER +KEL +Q EED+KN R+ +L KLQ K+K YK+Q EEAEE A NL+KFRK Q E +E+E+R+ AE
Sbjct: 146 KRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELLATDSAFDVLSFTAEEKAGVYKLTGAIMHYGNMK--FKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKSVYEKMFNWMVTRINATL--ETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKASDMTFKAKLYDNHLGKSNNFQKPRNVKGKQEAHFSLVHYAGTVDYNIMGWLEKNKDPLNETVVGLYQKSSLKLMATLFSTYASADTGDSGKGKGGKKKGSSFQTVSALHRENLNKLMTNLKTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPAAIPEGQFIDSRKGAEKLLGSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRIQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMANMKEEFGRVKDALEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMTERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQAEEDKVNTLTKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDISQQNSKIEDEQALALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSSRHDCDLLREQYEEEMEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKIRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHLRMVDSLQTSLDAETRSRNEALRVKKKMEGDLNEMEIQLSQANRIASEAQKHLKNSQAHLKDTQLQLDDAVHANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQTEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLMRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAE 1916 HSP 2 Score: 274.248 bits (700), Expect = 1.490e-72 Identity = 129/242 (53.31%), Postives = 168/242 (69.42%), Query Frame = 0
Query: 30 KSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKV--FKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGE------ASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+++P+D + C+VPD Y + + S +G KVT ET++ K K+DQV Q NPPKFD +DM+ LT+L++ VL+N RY +IYTYSGLFC+ +NPYK P+Y + Y GK+RSE PPHIF +++ +YQ M+ +NQSILITGESGAGKT NTK+VI YFAS+ A G R + E +LED+I+Q NP LEA+GNAKTVRNDNSSRFGKFIRI F GKL+ AD+
Sbjct: 27 QTRPFDIRTECFVPDDKEE-YVKAKVVSREGGKVTA---ETENGKTVTIKEDQVMQQNPPKFDKIEDMAMLTFLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRSKKENPNANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325302753|ref|XP_023332945.1| (myosin heavy chain, muscle-like [Eurytemora affinis]) HSP 1 Score: 2667.88 bits (6914), Expect = 0.000e+0 Identity = 1289/1767 (72.95%), Postives = 1537/1767 (86.98%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
RR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVISYFA++ +SGKRKEGE SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADMV+YLLEKSRLT+Q LERCYHAFYN+MSDAVPDLK+KCLL+DNI DYW+VSQGK++V SIDDKEDMQ+A +AF ILGF +EE Y+VYK T+ +MHMGNMTKDFVPVGKEEQAEIK + N++KVA + GID EWMINYFCKPKLKVGTEWV+KG +CS A++SVAGIAR IYE FR + DKCNETL DPTMKKVQYIG LDIAGFEIFDYNGFEQICIN+ NEKLQQFFN HMF LEQEEYVREG++WANVDFGMDLQKCIDMFEKPMGLLA+ EEESLFPKATDQ+F KLH NLL K PNF K +P+PDP AHFAV+HYAATVSYNLT+WLEKNKDPLNDT+VE+ KNGSN+L+I+CF DHPGQPLE KD GG +K GG KTVSS++KGQLDDLM TLY T+P FIRCVVPNTHK+PGGVE GL+MHQYQCNGVLAGIAICRKGFPNKM Y +FKARYNILAA VAKAK DK AA AVL + L+ EK+RLGHTKVFFRAGILG+MEEVRED++G VL+WLQS ARGKASRM FKK+QDQKLALY CQRTIRN+ IGKTWLWWQ+W IKP+LKCT+F +YK E+E+KIA+AE NI KA+ADR+KV+ +H+ LL QK+E++LAL SGGSAVQDIIDK RVE +A D+QKQ+ EV RI AE++Q+ S+ Q KV+SQ +QL+ E++ +E +L +EQ++ +KD+QIRTLK+E+ HQ +M+ KLG+EK+ D +QK EED+Q ++DK +HLS+VK KLEQ+LDEAEDALE EKK KND EK+KRK E D KLTQE + DLERVKAEL+Q + RKEKE++A++AKI+DE +LG KY KQ KELQ RLEELDEEL IER +RAKAEKSRS+LKKD+ED+ +RLEEAGANT TQVELNKKRE EL R+K E+EE NI HEGTLAALRMKHNNTMS+LGE ID+LN +K+KAEKDK +ERDL ++R+SLED V+++AEL+R GK++QGSI ++++KLDE+ARA+NEA+S KKRL +E DL RQIEE ENA+A NK K+SL TQLEDTKRL+++E++DRSSLLTKYK++TTE E+ RE+IENEH +KSD LK+LSKAQAEIQLW+SR+ETEGMGRVEELEG KL++RI EAEET+++L K+ + EKS+ R+ DL+E+S+EYER HAAA+I+EKRG+NFD+V+GEW+AKA D+A E+EASN ECRNY+SEL+R++A+H+EA EQLD+VKRENKNLADEI+DLLDQLGDGGRSIH+LDKQRRRLEVEKEELQ+ALEEAE ALEQEENKVLR+QLELGQVRQEIDR++ EK+EEF NTRKNH RAMDSL+ASLEAEQ+AK EALRIKKKLE DINELEIALD ANKAN+EG K +KRYQ QLR+++QG E+ESR RQ++ E VGIA+RK AL+ E+EESRALL S+ER KRQL+AEL ++R SVNEM SIN++ + KRA E +H IQAEID ++ AK+SEEK KRAMVDAARLADELR+EQ+HS +E R KR+LE+QLGELET+L +AE NA + G++A+S+LE ++RELE ELG Q RTSE+ K +Q+ ER +KEL FQQEED+KNQ++M+E+A KLQQKIK YKQQIEEAEEIAALNLAKFRK+QQ+FEE+E+R K A+++
Sbjct: 149 RRNECPPHIFAIAEGAYQGMMNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKRKEGEVSLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADMVVYLLEKSRLTYQQPLERCYHAFYNIMSDAVPDLKEKCLLTDNILDYWYVSQGKITVPSIDDKEDMQFADEAFDILGFTKEEKYDVYKNTACMMHMGNMTKDFVPVGKEEQAEIKDDTNSIKVATLAGIDPEWMINYFCKPKLKVGTEWVSKGSSCSNAANSVAGIARAIYERTFRLVVDKCNETLIDPTMKKVQYIGVLDIAGFEIFDYNGFEQICINYVNEKLQQFFNSHMFTLEQEEYVREGLDWANVDFGMDLQKCIDMFEKPMGLLAVFEEESLFPKATDQTFCEKLHSNLLGKWPNFAKPNPRPDPDAHFAVIHYAATVSYNLTNWLEKNKDPLNDTIVEMIKNGSNSLMIQCFADHPGQPLETPKDDGGRKKKGGG-KTVSSYFKGQLDDLMTTLYKTEPHFIRCVVPNTHKQPGGVEPGLIMHQYQCNGVLAGIAICRKGFPNKMIYAEFKARYNILAAKLVAKAKNDKAAAGAVLESINLDKEKYRLGHTKVFFRAGILGFMEEVREDKIGSVLAWLQSGARGKASRMAFKKLQDQKLALYCCQRTIRNFRIGKTWLWWQIWSAIKPHLKCTQFGKYKEEFEQKIALAEANIDKAIADRQKVQAIHDNLLSQKNEVSLALTSGGSAVQDIIDKAVRVENIANDIQKQVAEVKKRIKAEEEQKSSIEDQMRKVESQINQLQSEVSTLERQLENSEQERSDKDDQIRTLKDEIAHQADMLAKLGKEKKSGIDFRQKVEEDIQTMDDKCNHLSRVKGKLEQALDEAEDALEREKKSKNDVEKLKRKCEGDFKLTQEAVVDLERVKAELAQTLARKEKEYSALSAKIEDEGSLGSKYQKQIKELQGRLEELDEELNIERSNRAKAEKSRSILKKDIEDIAARLEEAGANTGTQVELNKKREGELSRLKCELEELNIAHEGTLAALRMKHNNTMSDLGENIDSLNSSKVKAEKDKSGLERDLADSRASLEDAVKSRAELERTGKLLQGSIAESHTKLDELARAMNEADSTKKRLTVENQDLSRQIEELENALANMNKSKVSLATQLEDTKRLSESESKDRSSLLTKYKHMTTEFETYREKIENEHLKKSDALKSLSKAQAEIQLWRSRYETEGMGRVEELEGNNSKLRSRITEAEETIDSLQTKLANIEKSKQRIGEDLDEISMEYERVHAAALITEKRGKNFDKVLGEWQAKASDIACEVEASNNECRNYSSELYRVKASHDEATEQLDIVKRENKNLADEIKDLLDQLGDGGRSIHDLDKQRRRLEVEKEELQSALEEAEGALEQEENKVLRAQLELGQVRQEIDRRLAEKDEEFLNTRKNHDRAMDSLNASLEAEQKAKGEALRIKKKLEGDINELEIALDHANKANSEGMKTIKRYQSQLRESLQGLENESRIRQQIAEQVGIAERKAAALSAEMEESRALLDSAERGKRQLEAELADTRNSVNEMQSINSKEIAVKRALEGNLHTIQAEIDSLLQAAKSSEEKGKRAMVDAARLADELRAEQDHSAAESRIKRSLETQLGELETKLTEAEENAIRCGKSALSRLETKVRELEQELGCTQVRTSENMKGFQKTERKIKELLFQQEEDKKNQDKMSEIANKLQQKIKVYKQQIEEAEEIAALNLAKFRKAQQDFEENEERVKLADEA 1914 HSP 2 Score: 478.019 bits (1229), Expect = 2.733e-134 Identity = 209/263 (79.47%), Postives = 232/263 (88.21%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGHIKKS GPDPDPSPWL VSPELK K K KPYDPKKS WV +KA GGY EGL++S DG KVTV + + KV+K+DQV QVNPPKFDCSDDM+ LT+L DACVLWNS +RY N+LIYTYSGLFCIAINPYKRFPIYT R++++Y+G+RR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVISYFA++ +SGKRKEGE SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADM
Sbjct: 1 MPGHIKKSSGPDPDPSPWLCVSPELKEKNKLKPYDPKKSVWVVNKADGGYLEGLLESKDGAKVTVNV--NGEMKVYKEDQVCQVNPPKFDCSDDMANLTFLGDACVLWNSTIRYVNQLIYTYSGLFCIAINPYKRFPIYTLRTMEIYVGRRRNECPPHIFAIAEGAYQGMMNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKRKEGEVSLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADM 261
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325302843|ref|XP_023332992.1| (myosin heavy chain, muscle-like [Eurytemora affinis]) HSP 1 Score: 2655.55 bits (6882), Expect = 0.000e+0 Identity = 1283/1765 (72.69%), Postives = 1517/1765 (85.95%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
RR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVI+YFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADMV+YLLEKSRLT+Q LERCYHAFYN+MSDAVPDLK+KCLLSDNI DYW+VSQGK+SV SIDDKEDM +A +AF +LGF E+E Y+VYK T+ +MHMGNMTKDFVPVGKEEQAEIK + NA+KVA + GID+EWMINYFCKPKLKVGTEWV KG TCS A++SVAGIAR IYE FR + +KCNETL DPTMKKVQYIG LDIAGFEIFDYNGFEQICIN+ NEKLQQFFNQHMF LEQEEYVREG++WANVDFGMDLQKCIDMFEKPMGLLAI EEESLFPKATDQSF KLH NLL K NF K +P+PDP AHFAV+HYAA VSYNLT WLEKNKDPLNDT+VE+ KNGSN+L+I+CF DHPGQPLE KD GG +K GG KTVSS++KGQLDDLM TLY T+P FIRCVVPNTHK+PGGVE GLVMHQYQCNGVLAGIAICRKGFPNKM Y +FKARYNILAA AVAK K DK AA AVL+ +KLE EK+RLGHTKVFFRAGILG+MEEVRED++GEVLSWLQ+ ARGKASRM FKK+QDQKLALY CQRTIRNYYIGKTWLWWQ+W+L+KP+LKC +F +YKAEYE+KIA+AE NI KA+A+ V +H+ L +K EL LAL SGGSAVQDIIDK R+EGM D+QKQ+DE RIAAE+ + Q KV + +LREEI +E E+D++ KD QI+TLKEE+ HQ E+I K+ REK+ V D +QK EED+Q++ED+ +HL+KVK KLEQSLDE EDALE EKK + D EK+KRKIE DLKLTQE +SDLERVKAELSQ +QRKEKE ++++AKI+DE TLGGKYSKQ KELQSR+EELDEEL IER +RAKAEK+RS+L +D+ED+G RLEEAG NT+TQ+ELNKKRE EL ++K+++EE NI HEGTLAA+R KHNNTMS++GEQID+LN K K++KDK MERDLQEAR+ L++ +R +A +++N KM Q I ++N+KLDE+ARALNEA+S K+L +E DL RQIEE ENA+A K K+SLTTQLEDTKRLADAE RDR+SLLTK+K L+TE+E+ R RIE E ERK+D L+ALSKAQAEIQLW+S+FETE +GR++ELE ++ KL R+ EAEET+++LN K+ ++EK++ R++ +LEEM +EYERTHAAA+I+EKRGRNFD+V+GEWKAKADDL E+EAS ECRNYNSE+FRL+AA+EE +EQLDVV+RENKNLADEI+DLLDQLGDGGRSIH+LDKQRRRLEVEKEELQAALEEAE+ALEQEENKVLR+QLELGQV+QEIDR+IQEKEEEF+NTRKNH RAMDS+ ASLE+EQRAK EALRIKKK+E DINELEIALD ANKAN E QK++KRYQGQLR++ YE+ESR R E+ E +ADR+ NAL GE+EE+R+LL S+ER K+Q +AEL ++R +VNEM++IN++A EKR E +H + AEIDDM+ QAKNSEEK+K+AM+DAARLADELR+EQ+H ++ + KRA E+QL ELE +L +A NA + GRAAM+KLE RIRELE+ELGS+QSRTS++AK +Q+ ER VKEL FQ +ED+KNQERM+ELA KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EESE+R++ AE
Sbjct: 149 RRNECPPHIFAIAEGAYQGMMNSGCNQSILITGESGAGKTENTKKVIAYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADMVVYLLEKSRLTYQQPLERCYHAFYNIMSDAVPDLKEKCLLSDNILDYWYVSQGKISVPSIDDKEDMMFADEAFDVLGFTEDEKYDVYKNTACMMHMGNMTKDFVPVGKEEQAEIKDDTNAIKVAALAGIDAEWMINYFCKPKLKVGTEWVNKGSTCSAAANSVAGIARAIYERSFRIVVEKCNETLIDPTMKKVQYIGVLDIAGFEIFDYNGFEQICINYVNEKLQQFFNQHMFTLEQEEYVREGLDWANVDFGMDLQKCIDMFEKPMGLLAIFEEESLFPKATDQSFCEKLHSNLLGKWENFAKPNPRPDPDAHFAVIHYAAVVSYNLTGWLEKNKDPLNDTIVEMIKNGSNSLMIQCFADHPGQPLETPKDDGGRKKKGGG-KTVSSYFKGQLDDLMTTLYKTEPHFIRCVVPNTHKQPGGVEPGLVMHQYQCNGVLAGIAICRKGFPNKMIYAEFKARYNILAAKAVAKCKNDKSAAGAVLDAIKLEKEKYRLGHTKVFFRAGILGFMEEVREDKIGEVLSWLQAGARGKASRMQFKKLQDQKLALYCCQRTIRNYYIGKTWLWWQIWMLVKPHLKCMQFGKYKAEYEKKIAVAEANIDKAIAECDAVIKIHDRLENEKQELHLALTSGGSAVQDIIDKTNRLEGMKNDLQKQVDETKKRIAAEEDVIAGIQQAGNKVTADAHRLREEIKNLENAAEKCEEDRITKDNQIKTLKEEISHQEELIAKMQREKKSVGDGRQKVEEDIQSMEDRCNHLAKVKGKLEQSLDECEDALEREKKCRGDVEKMKRKIEGDLKLTQEAVSDLERVKAELSQTIQRKEKELSSLSAKIEDEQTLGGKYSKQIKELQSRIEELDEELAIERQNRAKAEKNRSILSRDIEDIGRRLEEAGCNTSTQIELNKKRESELVKLKSDLEEANIAHEGTLAAIRAKHNNTMSDMGEQIDSLNKMKAKSDKDKAGMERDLQEARAGLDESMRDRANIEKNCKMTQALIVESNTKLDELARALNEADSSNKKLSVEHQDLIRQIEETENAIATLGKNKVSLTTQLEDTKRLADAEGRDRASLLTKFKGLSTEVENLRMRIEEEAERKNDVLRALSKAQAEIQLWRSKFETEALGRIDELESSKAKLNVRVQEAEETIDSLNTKVSATEKTKHRIEAELEEMQMEYERTHAAAVITEKRGRNFDKVVGEWKAKADDLLAELEASRTECRNYNSEVFRLKAAYEETVEQLDVVRRENKNLADEIKDLLDQLGDGGRSIHDLDKQRRRLEVEKEELQAALEEAESALEQEENKVLRAQLELGQVKQEIDRRIQEKEEEFDNTRKNHARAMDSMQASLESEQRAKAEALRIKKKIEGDINELEIALDHANKANNEAQKSIKRYQGQLRESECAYEEESRMRAEMTEKACLADRRANALHGEMEEARSLLDSAERGKKQTEAELADARNAVNEMNTINSKAASEKRRVEGIVHTMHAEIDDMLQQAKNSEEKSKKAMIDAARLADELRAEQDHVNTQSKAKRAFETQLVELENKLDEANENAMRGGRAAMAKLETRIRELEIELGSVQSRTSDNAKGHQKCERRVKELSFQIDEDKKNQERMSELATKLQQKIKTYKKQIEEAEEIAALNLAKFRKAQQELEESEERTRMAE 1912 HSP 2 Score: 486.108 bits (1250), Expect = 5.331e-137 Identity = 213/263 (80.99%), Postives = 236/263 (89.73%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGHIKK+ GPDPDPSPWLIVSPELK K K+KPYDPKKS WVP+KA GG+ EGLI+S DG K++V + + KVFK+DQV QVNPPKFDCSDDM+ LTYL DACVLWNSV+RY NELIYTYSGLFCIAINPYKRFPIYT R+++LY GKRR+ECPPHIF +AEG+YQGMMN G NQSILITGESGAGKTENTKKVI+YFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADM
Sbjct: 1 MPGHIKKTTGPDPDPSPWLIVSPELKEKNKAKPYDPKKSVWVPNKADGGFVEGLIESKDGAKISVNV--KGEVKVFKEDQVCQVNPPKFDCSDDMANLTYLGDACVLWNSVIRYVNELIYTYSGLFCIAINPYKRFPIYTYRTMELYTGKRRNECPPHIFAIAEGAYQGMMNSGCNQSILITGESGAGKTENTKKVIAYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNASGKLSGADM 261
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325305439|ref|XP_023334330.1| (myosin heavy chain, muscle-like [Eurytemora affinis]) HSP 1 Score: 2631.28 bits (6819), Expect = 0.000e+0 Identity = 1269/1765 (71.90%), Postives = 1518/1765 (86.01%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
RR+ECPPHIF +AEG+YQGM+N G NQSILITGESGAGKTENTKKVISYFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADMV+YLLEKSRLT+Q LERCYHAFYNLMSD VPDLK+KCLL+D+I DYW+VSQGK+SVESI+DKEDMQ+A +AF ILGF+++E Y+V+K T+ +MHMGNMTKDFVPVGK+EQAEIK E NA VA + GID+EWMI YFCKPKLKVGTEWV KG +C+ A+SSVAGIAR IYE FR + +KCNETL DPTMKKVQYIG LDIAGFEIFD+NGFEQICIN+ NEKLQQFFNQHMF LEQEEYVREG++WANVDFGMDLQKCIDMFEKPMGLLA+ EEESLFPKATDQ+F KLH NLL K PNF K +P+PDP AHFAV+HYAATVSYNLTSWLEKNKDPLNDT+VE+ KNGSN+L+I+CF DHPGQPLEA KD GG +K GG KTVSS++KGQLDDLM TLY T+P FIRCVVPNTHK+PGGVE GL+MHQYQCNGVLAGIAICRKGFPNKM Y +FKARYNILAA VAKAK DK AA AVL ++LE EK+RLGHTKVFFRAGILG+MEEVREDR+G+VL+WLQS ARGK+SRM FKK+QDQKLALY CQRTIRNYYIGKTWLWWQ+W+ IKPNLKCT+F +YKAEYE KIA+AE NI KA+A+ KV H+ LL +K+EL LAL SGG+AVQDIIDK R+E D+QKQ+D+ N RI E++ + Q KV + ++LR+EI +E + E+DK+ KD QIRTL++E+ HQ ++I KL +EK+ + +QK EED+Q++ED+ +HL+KVK KLEQSLDE ED LE EKK K D EK+KRK+E DLKLTQE SDLER+ AEL+Q VQRKEKE A++ AKI+DE TLGGKYSKQ KELQSR++ELDEE+++ER +RAKAEK+RS+L +DLED+G+RL EAG+NT+TQ+ELNKKRE ELH++KA++EE NI HEGTLAALR KHNN+MSELGEQID++N NK K+EKDK MERDLQEAR+ LE+ +R +A +++N KM Q I ++N KLDE+ARALNEA+S KK+L +E DL RQI+E ENA+A K KISLTTQLEDTKRLAD EARDR++LLTKYKNL+T+ E+ R +I+ E E+K+D L+ALSKAQAEIQLW+S+FETEG+GR+EELEG++ KL AR+ EAEE +E+LN+KI S+EKS+ R++ +LEEM++EYERTHAAA+I+EKRGRNFD+V+GEWKAKADD+ E+EAS+ ECRN+N+E FRL+AA +E+ EQLD+V+RENKNLADEI+DLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAE ALE EENKVLR+QLELGQVRQEIDR+I EKEEEFNN+RKNHQRAM+S+ ASLE+EQRAK+EALRIKKKLE DINELEIALD +NKAN E QK++KRYQ QLR++ YE+ SR RQE+ E +A+R+ NAL GE+EE+R+LL S+ER KRQ +AEL E+R +VNEM++IN+RA EKR E H +QAEIDDM+ QAKNSEEK+K+AMVDAARLADELR+EQ+H ++ + KRALE+Q+ ELE LA+A +A + GR AM+KLE R+RELE+ELG++Q RT ++AKA+Q+AER +KELQFQ +ED KNQERM++LA KLQ KIKTYK+QIEEAEEIAALNLAKFRKSQQE EE+E+R + A+
Sbjct: 149 RRNECPPHIFAIAEGAYQGMLNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNAAGKLSGADMVVYLLEKSRLTYQQPLERCYHAFYNLMSDQVPDLKEKCLLTDDIRDYWYVSQGKLSVESINDKEDMQFADEAFDILGFSQDEKYDVFKNTACMMHMGNMTKDFVPVGKDEQAEIKDEKNANIVATLMGIDAEWMITYFCKPKLKVGTEWVQKGSSCNNAASSVAGIARAIYERTFRLVVEKCNETLCDPTMKKVQYIGVLDIAGFEIFDFNGFEQICINYVNEKLQQFFNQHMFTLEQEEYVREGLDWANVDFGMDLQKCIDMFEKPMGLLAVFEEESLFPKATDQTFCEKLHANLLGKWPNFAKPNPRPDPDAHFAVLHYAATVSYNLTSWLEKNKDPLNDTIVEMIKNGSNSLMIQCFLDHPGQPLEAPKDDGGRKKKGGG-KTVSSYFKGQLDDLMTTLYKTEPHFIRCVVPNTHKQPGGVEPGLIMHQYQCNGVLAGIAICRKGFPNKMIYAEFKARYNILAAKLVAKAKNDKGAAGAVLEAIQLEKEKYRLGHTKVFFRAGILGFMEEVREDRIGQVLAWLQSGARGKSSRMQFKKLQDQKLALYCCQRTIRNYYIGKTWLWWQMWMAIKPNLKCTQFGKYKAEYEGKIALAEANIDKAIAECNKVVAEHDRLLNEKNELDLALNSGGNAVQDIIDKTNRIEAARNDLQKQVDQTNARIRGEEETIAGIQQSGIKVTGEATKLRDEIKNLENTIGKCEEDKMTKDNQIRTLRDEIAHQEDLIAKLSKEKKSTGEGRQKIEEDIQSMEDRCNHLNKVKGKLEQSLDECEDTLEREKKAKGDVEKMKRKVEGDLKLTQEATSDLERINAELAQTVQRKEKESASLYAKIEDEQTLGGKYSKQVKELQSRIDELDEEIIVERNNRAKAEKNRSLLSRDLEDIGTRLAEAGSNTSTQIELNKKREAELHKLKADLEEANIAHEGTLAALRQKHNNSMSELGEQIDSINKNKAKSEKDKAGMERDLQEARTGLEEAMRERANMEKNCKMTQSLIVESNQKLDELARALNEADSTKKKLMVESQDLSRQIDETENAIAALQKNKISLTTQLEDTKRLADGEARDRAALLTKYKNLSTDAENLRMKIDEESEKKNDVLRALSKAQAEIQLWRSKFETEGLGRIEELEGSKHKLAARVSEAEEAIESLNSKISSAEKSKHRIESELEEMAMEYERTHAAAVITEKRGRNFDKVVGEWKAKADDIQAELEASHSECRNFNAEGFRLKAALDESNEQLDIVRRENKNLADEIKDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEGALESEENKVLRAQLELGQVRQEIDRRIAEKEEEFNNSRKNHQRAMESMQASLESEQRAKSEALRIKKKLEGDINELEIALDHSNKANNEAQKSIKRYQCQLRESECAYEEASRVRQEMSEKASLAERRANALQGEMEEARSLLDSAERGKRQTEAELAEARNAVNEMTTINSRASAEKRHIEGNCHTMQAEIDDMLHQAKNSEEKSKKAMVDAARLADELRAEQDHVNTQAKAKRALETQMNELEQNLAEANEHAMRGGRTAMAKLETRVRELEIELGNVQGRTGDNAKAHQKAERKIKELQFQNDEDHKNQERMSDLASKLQAKIKTYKKQIEEAEEIAALNLAKFRKSQQELEETEERVRMAD 1912 HSP 2 Score: 479.559 bits (1233), Expect = 9.333e-135 Identity = 210/263 (79.85%), Postives = 233/263 (88.59%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGHIKK+ GPDPDPSPWL+VSPELK K K KPYDPKKS WV +KA GGY EGL++S DG+K TV + + KVFK+DQV QVNPPKFDCSDDM+ LTYL DACVLWNS +RY N+LIYTYSGLFCIAINPYKRFPIYT R+++LY GKRR+ECPPHIF +AEG+YQGM+N G NQSILITGESGAGKTENTKKVISYFA++ +SGK+KEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FN GKLSGADM
Sbjct: 1 MPGHIKKTTGPDPDPSPWLVVSPELKDKNKLKPYDPKKSVWVKNKADGGYLEGLLESKDGNKWTVNV--KGEMKVFKEDQVCQVNPPKFDCSDDMANLTYLGDACVLWNSTIRYVNQLIYTYSGLFCIAINPYKRFPIYTLRTMELYTGKRRNECPPHIFAIAEGAYQGMLNSGMNQSILITGESGAGKTENTKKVISYFATICSSGKKKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNAAGKLSGADM 261
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1325281011|ref|XP_023325178.1| (myosin heavy chain, muscle-like [Eurytemora affinis] >gi|1325281013|ref|XP_023325179.1| myosin heavy chain, muscle-like [Eurytemora affinis] >gi|1325281015|ref|XP_023325180.1| myosin heavy chain, muscle-like [Eurytemora affinis] >gi|1325281017|ref|XP_023325181.1| myosin heavy chain, muscle-like [Eurytemora affinis]) HSP 1 Score: 2404.02 bits (6229), Expect = 0.000e+0 Identity = 1216/1767 (68.82%), Postives = 1473/1767 (83.36%), Query Frame = 0
Query: 264 RRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSED--NAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDPHAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGGKTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAE 2028
RR+E PPHIF +AEG++QGMM GKNQSILITGESGAGKTENTKKVISYFAS+GA+GK+KEGE LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FNQ GKL+GADMVIYLLEKSRLT+Q LERCYH+FYNLMSDAVPDLK KCLLS+N+ DY++VSQGK++V+SIDDKEDMQ+A +AF ILGF +EE Y+VYK T+ +MHMG + K F+ VGKEEQA+IK ED +A V+ + GID+EWMINYFCKPKLKVG EWVTKGQT SQASSSVAGIAR +YE F+FI DKCN+TL DPTMK+VQ+IG LDIAGFEIFDYNGFEQICINFCNEKLQQFFN HMFVLEQEEY++EGIEWA VDFGMDLQKCI+MFEKPMGLLAILEEESLFPKATDQSFAAKLH NLL KCPNF K PKPDP AHFAV HYAA VSYNLTSWLEKNKDPLNDT+VE+ KNG+N L + CF DHPGQP E KD GG +K GG KTVSSFYK QL+DLMKTL+AT+P FIRCVVPNTHK+PGG+E L+MHQ CNGVL GI ICRKGFPN+M+Y DFK+RYNILAA VAKAK DK AA AVL V LE EK+RLGHTKVFFRAG+LG+MEEVREDRVG+VLSWLQS RGK SRM F KMQ QKL+LY CQRTIRNY IGKTWLWWQ+W+ +KPNLKCTKF+QYKAEYEEKIAIAE NI KA+A+ KV++ H L +K+++ +L+SGG V+++ K ++E D++KQ+D++N RI +E+ R S Q K++ +LR+EI ++T + +E+D V K+ QIRTLKEEL HQ E+I KL REK+ +++QKTEE +QA EDK +HLSK+K KLEQSLDE+ED+LE EKK + D+EK+K+K+ESDLKLTQET++DL+R+K EL+ +QRKEKE+A++ AKI+DE TLGGKYSKQ KELQ+R+EELDEEL IER +R KAEK+R+ L +D+EDLG +LE+AG NT TQ+ELNKKRE EL ++KA++EE NI EG LA R KHN +SE+G QID+LN K KAE DK MERDL EA++SL+ +R + +++ GK+ Q I ++N +LD+MARALNEA+S KK+L +E DL+RQIE+ E +A+ K KISLTTQLEDT+RLADAE RDR+ L++K+KNL +ELE+ R RIE E E K + LK LSKAQ+EIQLWKS++ETEG+GR++ELEG + KL R+ EAEET+E+L+ K+ S+EKS+ R+ +LE++ LE+ER +AAAI+++KR +NFD+V+GEWK K DDL+ E+EAS +E RNYNSE+FRLRA EE EQLD VKRENKNLA+EI+DLLDQLG+GGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKV+R+QLEL QVRQEIDR+IQEKEEEF NTRKN+QRA+DS+ AS+EAE RAK EALRIKKKLE DINELEIALD ANK+NAE K +KRYQ Q R+ YE+ESR RQE+ + G+ADRK NAL GE+EESR+LL S+ER K+Q + EL ++R +VN+M++IN RA +EKR ES +H + AEID ++ KNSEEKAK+AMVDA+RLA+ELRSEQ+H +S+ + +RAL++Q+ ELE RL + +A K G+ A++KLE RIRELEMELG+ Q RTSE+ K+YQ++ER +KELQFQ +ED+KNQE+M ELA KLQ KI+TYK+QIEEAEEIAALNLAKFRK+QQE EE+E+RS+ AE
Sbjct: 149 RRNEVPPHIFAIAEGAFQGMMVGGKNQSILITGESGAGKTENTKKVISYFASIGATGKKKEGEPGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNQSGKLAGADMVIYLLEKSRLTYQQPLERCYHSFYNLMSDAVPDLKAKCLLSNNVRDYFYVSQGKIAVDSIDDKEDMQFADEAFDILGFTKEEKYDVYKNTACLMHMGELAKSFIGVGKEEQADIKEEDQKHAKNVSTLLGIDNEWMINYFCKPKLKVGAEWVTKGQTVSQASSSVAGIARSLYERTFKFIVDKCNDTLCDPTMKRVQFIGVLDIAGFEIFDYNGFEQICINFCNEKLQQFFNHHMFVLEQEEYMKEGIEWAMVDFGMDLQKCIEMFEKPMGLLAILEEESLFPKATDQSFAAKLHANLLGKCPNFAKPDPKPDPEAHFAVCHYAAKVSYNLTSWLEKNKDPLNDTIVEMIKNGTNALCVLCFLDHPGQPAETPKDQGGSKKKGGG-KTVSSFYKSQLEDLMKTLHATEPHFIRCVVPNTHKQPGGIEPALIMHQLTCNGVLEGIRICRKGFPNRMSYPDFKSRYNILAAGLVAKAKNDKAAASAVLETVGLEKEKYRLGHTKVFFRAGVLGHMEEVREDRVGQVLSWLQSYCRGKQSRMNFVKMQGQKLSLYCCQRTIRNYMIGKTWLWWQIWITLKPNLKCTKFAQYKAEYEEKIAIAEANIDKAIAECNKVKSAHAKLDEEKADVKRSLESGGDVVKELSAKIEKLEKSRNDLEKQVDQLNTRIRSEEDTRRSAEQTGSKLKKDTDKLRDEIRDVQTNIEKSEEDVVTKESQIRTLKEELAHQEELIQKLTREKKTAGESRQKTEEGIQAAEDKCNHLSKLKLKLEQSLDESEDSLEREKKARADAEKIKKKVESDLKLTQETVADLDRLKEELNSTIQRKEKEFASVAAKIEDEQTLGGKYSKQIKELQTRIEELDEELAIERNNRNKAEKNRATLSRDIEDLGEKLEDAGNNTQTQIELNKKREAELMKLKADMEESNISFEGILANTRSKHNGVISEMGSQIDDLNKAKAKAEADKARMERDLHEAKTSLDAAIRERQNVEKEGKLTQSKIAESNQRLDDMARALNEADSSKKKLTVEGQDLQRQIEDSEAHIAELGKAKISLTTQLEDTRRLADAETRDRAGLMSKFKNLNSELENLRARIEEEAEHKGEILKQLSKAQSEIQLWKSKYETEGLGRIDELEGNKAKLMMRLQEAEETIESLHTKVASTEKSKHRMDTELEDLQLEFERVNAAAIVADKRAQNFDKVVGEWKMKVDDLSSELEASQRESRNYNSEVFRLRAGWEEITEQLDAVKRENKNLAEEIKDLLDQLGEGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVMRAQLELSQVRQEIDRRIQEKEEEFENTRKNYQRALDSMQASMEAETRAKAEALRIKKKLEADINELEIALDHANKSNAEAHKTIKRYQNQYREAETAYEEESRQRQEIVDKAGLADRKANALLGELEESRSLLDSAERGKKQAELELHDARVAVNDMTTINGRAANEKRQLESAVHTLHAEIDSILQSVKNSEEKAKKAMVDASRLAEELRSEQDHCSSQLKARRALDAQVKELELRLEEVVEHATKGGKNAIAKLEGRIRELEMELGNSQMRTSETLKSYQKSERKIKELQFQNDEDQKNQEKMGELATKLQDKIRTYKKQIEEAEEIAALNLAKFRKAQQELEEAEERSRLAE 1914 HSP 2 Score: 477.248 bits (1227), Expect = 4.564e-134 Identity = 209/263 (79.47%), Postives = 236/263 (89.73%), Query Frame = 0
Query: 1 MPGHIKKSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEASLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
MPGHIKK GPDPDPS WL VS ELK K K+KPYDPKKSCWVP+KA+GG+ EG+I+ST+GDK+TV++ +KKVFKKD VGQVNPPKFDC +DMS LTYLNDA VLWN +RY NELIYTYSGLFCIA+NPYKRFPIYTQR++++Y+GKRR+E PPHIF +AEG++QGMM GKNQSILITGESGAGKTENTKKVISYFAS+GA+GK+KEGE LEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRI FNQ GKL+GADM
Sbjct: 1 MPGHIKKGSGPDPDPSQWLFVSYELKEKNKAKPYDPKKSCWVPEKATGGFAEGMIESTEGDKITVSV--KGEKKVFKKDLVGQVNPPKFDCCEDMSNLTYLNDASVLWNLRIRYVNELIYTYSGLFCIAVNPYKRFPIYTQRAMEVYMGKRRNEVPPHIFAIAEGAFQGMMVGGKNQSILITGESGAGKTENTKKVISYFASIGATGKKKEGEPGLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIHFNQSGKLAGADM 261
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022994|ref|XP_023023736.1| (myosin heavy chain, muscle isoform X48 [Leptinotarsa decemlineata]) HSP 1 Score: 1898.63 bits (4917), Expect = 0.000e+0 Identity = 992/1789 (55.45%), Postives = 1339/1789 (74.85%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K KCLLS+NI DY++VSQGK ++ +DD E+M+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFD+NGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGIEWA +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY IL+A+A+ + AA+ +L+ + L+ E++RLG TKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K KAE+D+ N+ +LQ+ R ++E R KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWAFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILSASAIRDNMTPEKAAQVILDHINLDPEQYRLGKTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 353.214 bits (905), Expect = 1.987e-94 Identity = 166/261 (63.60%), Postives = 200/261 (76.63%), Query Frame = 0
Query: 7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKKDQV QVNPPK++ +DMS LTYLNDA VL N RY +LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022910|ref|XP_023023693.1| (myosin heavy chain, muscle isoform X6 [Leptinotarsa decemlineata]) HSP 1 Score: 1896.32 bits (4911), Expect = 0.000e+0 Identity = 992/1789 (55.45%), Postives = 1334/1789 (74.57%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K KCLLS+NI DY++VSQGK ++ +DD E+M+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFDYNGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGI W +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY IL A V+K K + +L+ L++E +RLGHTKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K KAE+D+ N+ +LQ+ R ++E R KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYTREGIVWQFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILNPAGVSKESDPKKCGQIILDASGLDSELYRLGHTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 352.829 bits (904), Expect = 3.003e-94 Identity = 166/259 (64.09%), Postives = 199/259 (76.83%), Query Frame = 0
Query: 9 DGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKKDQV QVNPPK++ +DMS LTYLNDA VL N RY +LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 8 EGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022930|ref|XP_023023703.1| (myosin heavy chain, muscle isoform X16 [Leptinotarsa decemlineata]) HSP 1 Score: 1895.94 bits (4910), Expect = 0.000e+0 Identity = 991/1789 (55.39%), Postives = 1337/1789 (74.73%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K KCLLS+NI DY++VSQGK ++ +DD E+M+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFDYNGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGI W +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY IL+A+A+ + AA+ +L+ + L+ E++RLG TKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K KAE+D+ N+ +LQ+ R ++E R KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYTREGIVWQFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILSASAIRDNMTPEKAAQVILDHINLDPEQYRLGKTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 353.214 bits (905), Expect = 2.443e-94 Identity = 166/261 (63.60%), Postives = 200/261 (76.63%), Query Frame = 0
Query: 7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKKDQV QVNPPK++ +DMS LTYLNDA VL N RY +LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022932|ref|XP_023023704.1| (myosin heavy chain, muscle isoform X17 [Leptinotarsa decemlineata]) HSP 1 Score: 1892.09 bits (4900), Expect = 0.000e+0 Identity = 996/1789 (55.67%), Postives = 1341/1789 (74.96%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K KCLLS+NI DY++VSQGK ++ +DD E+M+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFD+NGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGIEWA +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY ILA A +AK K+ K+AA+ L + L+A+ +R+GHTKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K KAE+D+ N+ +LQ+ R ++E R KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWAFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYMILAPATMAKEKEAKEAARKCLEEIGLDADSYRVGHTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 347.821 bits (891), Expect = 9.270e-93 Identity = 163/261 (62.45%), Postives = 200/261 (76.63%), Query Frame = 0
Query: 7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKK+ V QVNPPK++ +DM+ LTYLNDA VL N RY ++LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKHFKKENVHQVNPPKYEKVEDMADLTYLNDASVLHNLKQRYYSKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022920|ref|XP_023023698.1| (myosin heavy chain, muscle isoform X11 [Leptinotarsa decemlineata]) HSP 1 Score: 1890.93 bits (4897), Expect = 0.000e+0 Identity = 996/1789 (55.67%), Postives = 1342/1789 (75.01%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K+KCLLS++IYDY +VSQGKV++ ++DD E+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFD+NGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGIEWA +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY ILA A +AK K+ K+AA+ L + L+A+ +R+GHTKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K KAE+D+ N+ +LQ+ R ++E R KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKEKCLLSNDIYDYNYVSQGKVTIPNVDDGEECALTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDFNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYQREGIEWAFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYMILAPATMAKEKEAKEAARKCLEEIGLDADSYRVGHTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKAERDRANIYSELQQTRGAVEQVGREKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 353.214 bits (905), Expect = 2.184e-94 Identity = 166/261 (63.60%), Postives = 200/261 (76.63%), Query Frame = 0
Query: 7 KSDGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+ +G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKKDQV QVNPPK++ +DMS LTYLNDA VL N RY +LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 6 QQEGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264
BLAST of myosin heavy muscle isoform x20 vs. nr
Match: gi|1285022924|ref|XP_023023700.1| (myosin heavy chain, muscle isoform X13 [Leptinotarsa decemlineata]) HSP 1 Score: 1887.46 bits (4888), Expect = 0.000e+0 Identity = 988/1789 (55.23%), Postives = 1334/1789 (74.57%), Query Frame = 0
Query: 250 IWFNQGGKLSGADMRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADMVIYLLEKSRLTFQAELERCYHAFYNLMSDAVPDLKQKCLLSDNIYDYWWVSQGKVSVESIDDKEDMQYAHDAFRILGFNEEETYNVYKLTSVVMHMGNMTKDFVPVGKEEQAEIKSEDNAVKVAEICGIDSEWMINYFCKPKLKVGTEWVTKGQTCSQASSSVAGIARKIYELVFRFICDKCNETLFDPTMKKVQYIGCLDIAGFEIFDYNGFEQICINFCNEKLQQFFNQHMFVLEQEEYVREGIEWANVDFGMDLQKCIDMFEKPMGLLAILEEESLFPKATDQSFAAKLHENLLSKCPNFQKASPKPDP---HAHFAVVHYAATVSYNLTSWLEKNKDPLNDTVVELFKNGSNNLLIECFRDHPGQPLEAKKDAGGGGRKKGGG-KTVSSFYKGQLDDLMKTLYATDPSFIRCVVPNTHKKPGGVESGLVMHQYQCNGVLAGIAICRKGFPNKMTYDDFKARYNILAAAAVAKAKKDKDAAKAVLNVVKLEAEKFRLGHTKVFFRAGILGYMEEVREDRVGEVLSWLQSQARGKASRMVFKKMQDQKLALYSCQRTIRNYYIGKTWLWWQLWLLIKPNLKCTKFSQYKAEYEEKIAIAEKNIGKALADRKKVETMHEFLLGQKSELTLALKSGGSAVQDIIDKNTRVEGMAADVQKQLDEVNNRIAAEKQQRDSLTQQQGKVQSQQSQLREEINAMETRLATAEQDKVEKDEQIRTLKEELEHQTEMITKLGREKRGVQDNKQKTEEDVQALEDKSSHLSKVKHKLEQSLDEAEDALEHEKKVKNDSEKVKRKIESDLKLTQETISDLERVKAELSQAVQRKEKEWAAMNAKIDDENTLGGKYSKQTKELQSRLEELDEELMIERGSRAKAEKSRSMLKKDLEDLGSRLEEAGANTATQVELNKKREQELHRIKAEIEERNIGHEGTLAALRMKHNNTMSELGEQIDNLNGNKMKAEKDKGNMERDLQEARSSLEDGVRAKAELDRNGKMIQGSIGDANSKLDEMARALNEAESQKKRLEMEKMDLERQIEEGENAMAQFNKQKISLTTQLEDTKRLADAEARDRSSLLTKYKNLTTELESSRERIENEHERKSDHLKALSKAQAEIQLWKSRFETEGMGRVEELEGTRGKLQARIGEAEETVEALNNKIVSSEKSRSRLQVDLEEMSLEYERTHAAAIISEKRGRNFDRVIGEWKAKADDLAHEIEASNKECRNYNSELFRLRAAHEEAIEQLDVVKRENKNLADEIRDLLDQLGDGGRSIHELDKQRRRLEVEKEELQAALEEAEAALEQEENKVLRSQLELGQVRQEIDRKIQEKEEEFNNTRKNHQRAMDSLSASLEAEQRAKTEALRIKKKLEHDINELEIALDQANKANAEGQKAVKRYQGQLRDTIQGYEDESRFRQEVQEAVGIADRKGNALAGEVEESRALLVSSERSKRQLDAELEESRGSVNEMSSINTRAMHEKRATESGIHAIQAEIDDMMTQAKNSEEKAKRAMVDAARLADELRSEQEHSTSEDRHKRALESQLGELETRLADAEANAAKMGRAAMSKLEMRIRELEMELGSIQSRTSESAKAYQRAERHVKELQFQQEEDRKNQERMTELAQKLQQKIKTYKQQIEEAEEIAALNLAKFRKSQQEFEESEDRSKTAEKS 2030
++ N+ KL RR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+ YLLEK+R+ Q LER YH FY +MS AV +K KCLLS+NI DY++VSQGK ++ +DD E+M+ +AF ILGF +EE NVYK+T+ VMHMG M F G+EEQAE + +V ++ G+D+ + F KP++KVG E+VT+G+ +Q S SV +++ +++ VF+F+ KCNETL D K+ +IG LDIAGFEIFDYNGFEQ+CINF NEKLQQFFN HMFVLEQEEY REGI W +DFGMDL CI++ EKPMG+L+ILEEES+FPKATD++F KL+ N L K PNFQK P P P AHF + HYA V YN+T WLEKNKDPLNDTVV+LFK GSN LL+E F DHPGQ A + GG R KG +TVSS Y+ QL++LM TL +T P F+RC++PN K+PG ++S LVMHQ CNGVL GI ICRKGFPN+M Y DFK RY IL+A+A+ + AA+ +L+ + L+ E++RLG TKVFFRAG+LG MEE+R++R+G++++W+QS RG SR FKK+Q+Q+LAL CQR +R Y +TW W++LW ++P L T+ A+ EEK A A++ + +K++E ++ LL +K+EL L+ ++ ++ ++ +++ D++ QL E +R++ E+ R+ L QQ+ K++ + S +++I +E L +EQDK KD QIR L +E+ HQ E+I KL +EK+ +N QK E++QA EDK +HL+KVK KLEQ+LDE ED+LE EKK++ D EK KRK+E DLKLTQE ++DLER K EL Q +QRK+KE +++ AK++DE ++ GK KQ KELQ+R+EEL+EE+ ER +RAKAEK R+ L ++LE+LG RLEEAG T+ Q+ELNKKRE EL +++ ++EE NI HEGTLA LR KHN+ +SE+GEQID LN K K+EK+K +L + R+S++ KA +++ K + + D KLDE R LN+ ++ KK+L +E DL RQ+EE E+ ++Q +K K+SLTTQLEDTKRLAD E R+R++LL K++NL +L++ RE++E E E K+D + LSKA AE QLW+ ++E+EG+ R EELE + KLQAR+ EAEET+E+LN K+V+ EK++ RL ++E++ LE +R +A A +EK+ + FD++IGEWK K DDLA E++AS KECRNY++ELFRL+ A+EE EQL+ V+RENKNLADE++DLLDQ+G+GGR+IHE++K R+RLE EK+ELQAALEEAEAALEQEENKVLRSQLEL QVRQEIDR+IQEKEEEF NTRKNHQRA+DS+ ASLEAE + K EALR+KKKLE DINELEIALD ANKANAE QK +KRYQ QL+DT E+E R R E +E +GI++R+ NAL E+EESR LL ++R++RQ + EL ++ +N++ + N KR E+ + + +++D+++ +AKNSEEKAK+AMVDAARLADELR+EQ+H+ ++++ ++ALESQ+ +L+ RL +AEANA K G+ A++KLE R+RELE EL Q R +++ K +++ER +KEL FQ EEDRKN ERM +L KLQQKIKTYK+QIEEAEEIAALNLAKFRK+QQE EE+E+R+ AE++
Sbjct: 135 VYTNRCAKLYRGK-RRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADIETYLLEKARVISQQSLERSYHIFYQIMSGAVAGVKDKCLLSNNINDYYFVSQGKTTIPGVDDSEEMKVTDEAFDILGFTQEEKDNVYKITAAVMHMGCMK--FKQRGREEQAEPDGTEEGERVGKLLGVDTAALYQAFVKPRIKVGNEFVTQGRNVNQVSYSVGAMSKAMFDRVFKFLVKKCNETL-DTKQKRQHFIGVLDIAGFEIFDYNGFEQLCINFTNEKLQQFFNHHMFVLEQEEYTREGIVWQFIDFGMDLAACIELIEKPMGILSILEEESMFPKATDKTFEEKLNTNHLGKSPNFQKPKP-PKPGQQAAHFTLGHYAGNVPYNITGWLEKNKDPLNDTVVDLFKKGSNKLLVEIFADHPGQSGGAAEK--GGKRTKGSAFQTVSSLYREQLNNLMTTLRSTQPHFVRCIIPNELKQPGVIDSHLVMHQLTCNGVLEGIRICRKGFPNRMVYPDFKLRYKILSASAIRDNMTPEKAAQVILDHINLDPEQYRLGKTKVFFRAGVLGQMEELRDERLGKIVTWMQSWVRGYLSRKEFKKLQEQRLALQVCQRNLRKYLKLRTWPWYKLWQKVRPLLNVTRIEDEIAKLEEKAAKAQEAFEREEKAKKELEALYAKLLAEKTELLSNLEGKAGSLSEVQERANKLQAQKNDLEAQLSETQDRLSQEEDARNQLMQQKKKLEQEMSGYKKDIEDLELNLQKSEQDKATKDHQIRNLNDEIAHQDELINKLNKEKKLSGENNQKISEELQAAEDKVNHLNKVKAKLEQTLDELEDSLEREKKLRGDVEKSKRKVEGDLKLTQEAVADLERNKKELEQTIQRKDKEISSLTAKLEDEQSVVGKQQKQIKELQARIEELEEEVEAERQARAKAEKQRADLARELEELGERLEEAGGATSAQIELNKKREAELAKLRRDLEEANIQHEGTLANLRKKHNDAVSEMGEQIDQLNKLKAKSEKEKAQYFGELNDLRASVDHLANEKAAIEKVSKQLGQQLNDVQGKLDETNRTLNDFDAAKKKLSIENSDLLRQLEEAESQVSQLSKIKVSLTTQLEDTKRLADEEGRERATLLGKFRNLEHDLDNIREQVEEEAEAKADIQRQLSKANAEAQLWRQKYESEGIARSEELEEAKRKLQARLAEAEETIESLNQKVVALEKTKQRLATEVEDLQLEVDRANAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDASQKECRNYSTELFRLKGAYEEGQEQLEAVRRENKNLADEVKDLLDQIGEGGRNIHEIEKARKRLEAEKDELQAALEEAEAALEQEENKVLRSQLELSQVRQEIDRRIQEKEEEFENTRKNHQRALDSMQASLEAEAKGKAEALRMKKKLEADINELEIALDHANKANAEAQKTIKRYQQQLKDTQTALEEEQRARDEAREQLGISERRANALQNELEESRTLLEQADRARRQAEQELGDAHEQLNDLGAQNASLSAAKRKLETELQTLHSDLDELLNEAKNSEEKAKKAMVDAARLADELRAEQDHAQTQEKLRKALESQIKDLQVRLDEAEANALKGGKKAIAKLEQRVRELENELDGEQRRHADAQKNLRKSERRIKELSFQAEEDRKNHERMQDLVDKLQQKIKTYKRQIEEAEEIAALNLAKFRKAQQELEEAEERADLAEQA 1916 HSP 2 Score: 353.214 bits (905), Expect = 2.057e-94 Identity = 166/259 (64.09%), Postives = 199/259 (76.83%), Query Frame = 0
Query: 9 DGPDPDPSPWLIVSPELKAKLKSKPYDPKKSCWVPDKASGGYFEGLIDSTDGDKVTVTILETKDKKVFKKDQVGQVNPPKFDCSDDMSGLTYLNDACVLWNSVVRYKNELIYTYSGLFCIAINPYKRFPIYTQRSIDLYIGKRRSECPPHIFGVAEGSYQGMMNVGKNQSILITGESGAGKTENTKKVISYFASVGASGKRKEGEA----SLEDKIVQTNPVLEAWGNAKTVRNDNSSRFGKFIRIWFNQGGKLSGADM 263
+G DPDP+P+L VS E K ++KPYD KKSCWVPD G + G I T GD VTV L ++K FKKDQV QVNPPK++ +DMS LTYLNDA VL N RY +LIYTYSGLFC+AINPYKRFP+YT R LY GKRR+E PPHIF +++G+Y M+ +NQS+LITGESGAGKTENTKKVI+YFA+VGAS K+ ++ +LED++VQTNPVLEA+GNAKTVRNDNSSRFGKFIRI F GKL+GAD+
Sbjct: 8 EGDDPDPTPYLFVSLEQKRIDQTKPYDAKKSCWVPDDKEG-FLLGEIKGTKGDLVTVG-LPGGEEKNFKKDQVTQVNPPKYEKCEDMSNLTYLNDASVLHNLKQRYYAKLIYTYSGLFCVAINPYKRFPVYTNRCAKLYRGKRRNEVPPHIFAISDGAYVNMLTNHENQSMLITGESGAGKTENTKKVIAYFATVGASTKKATDDSVKKGNLEDQVVQTNPVLEAFGNAKTVRNDNSSRFGKFIRIHFGPTGKLAGADI 264 The following BLAST results are available for this feature:
BLAST of myosin heavy muscle isoform x20 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 25
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BLAST of myosin heavy muscle isoform x20 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of myosin heavy muscle isoform x20 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold286_size222086:148956..164589+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>snap_masked-scaffold286_size222086-processed-gene-1.7 ID=snap_masked-scaffold286_size222086-processed-gene-1.7|Name=myosin heavy muscle isoform x20|organism=Tigriopus kingsejongensis|type=gene|length=15634bp|location=Sequence derived from alignment at scaffold286_size222086:148956..164589+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'myosin heavy muscle isoform x20' has the following synonyms
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