BLAST of 3-hydroxyisobutyrate dehydrogenase vs. L. salmonis genes
Match: EMLSAG00000012782 (supercontig:LSalAtl2s:LSalAtl2s96:719242:725893:1 gene:EMLSAG00000012782 transcript:EMLSAT00000012782 description:"maker-LSalAtl2s96-augustus-gene-7.29")

HSP 1 Score: 48.9062 bits (115), Expect = 6.851e-7
Identity = 61/287 (21.25%), Postives = 115/287 (40.07%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDC---------------STVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEGG----KDFSAMLLR 268
            +  NL+ +GH VS ++      +    V ++ A+   A++++    + +I  S  ++  P AA      +C               + +D  +++D+ +     G   L+A V G    +  GTL  +A G    F   +     MG+ + + GE G      +   ++  +++ G    + LAD+ GL +  + +V+  +   C  +     A   G   P ++  Q       M KDL LS    +Q+    PL A A E    +  D   G      F+ M ++
Sbjct:  250 IVKNLLNSGHHVSVWNRTSEKCEEFVKVGALKALTP-ADVIL----DSDITFSCVSD--PQAAKDMVFGNCGVLTEISSTKAYVEMTGIDADTSQDIAEAICLKGGRYLEAQVQGSKQQSNDGTLVILAAGDRTLFDDCQSXFQAMGKNSFYLGEVGTASKMNLVLQLMAGVTLAGLAXGMALADRAGLQQXDVLEVLELTGLSCPLIIGKGKAIIEG-GFPTNHPLQ------HMQKDLKLSLNMGDQLEQPLPLTASANEKVCKVTKDQSDGSLKLNKFTGMKIK 522          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. SwissProt
Match: gi|2494062|sp|P77161.1|GLXR_ECOLI (RecName: Full=2-hydroxy-3-oxopropionate reductase; AltName: Full=Tartronate semialdehyde reductase; Short=TSAR)

HSP 1 Score: 103.605 bits (257), Expect = 6.371e-25
Identity = 87/283 (30.74%), Postives = 133/283 (47.00%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVD----GVQSVASIAAVAEGAELVISMLPNGNILRSV-YAE--LIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLD----RQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEGGKDFSAML--LRLM 270
            MA NL  AGH +    +  V  +    G  SV +   V E ++++  M+P+   +  V + E     A+  G  ++D S++     +   +Q +E G   LDAPVSGG  GA  GTL+ M GG +A F  V+PL  ++G+     G  G GQ  K+ N +++A+++    EA+  A K G D    RQ L        G+  S         V         + PGF  AL  KDL L+ Q+A+ ++   P  A   EL+ +   +G    D SA++  L LM
Sbjct:   15 MAINLARAGHQLHVTTIGPVADELLSLGAVSVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKRFARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVGGNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALM------GGFASSRILE-----VHGERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDHSALVQALELM 286          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. SwissProt
Match: gi|449061823|sp|F4I907.1|GLYR2_ARATH (RecName: Full=Glyoxylate/succinic semialdehyde reductase 2, chloroplastic; Short=AtGLYR2; Short=AtGR2; Short=SSA reductase 2; Flags: Precursor)

HSP 1 Score: 104.76 bits (260), Expect = 7.783e-25
Identity = 88/283 (31.10%), Postives = 128/283 (45.23%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVD-----GVQSVASIAAVAEGAELVISML------------PNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEGGKDFSAML 266
            MA NL+ AG  V+ ++      D     G +  +S   V    +L  +ML             NG I            ++G   +D STVDV S+  + +Q  + G L L+APVSG    A  G L F+  G    +    P L++MG+   + GE G G A K+  NM++   M    E I L+ K+GLD   L +VVS       ++NA  P   +   S   + Y   F      KD+ L+   AE VS +TP+ A A ELY+     G   +DFSA++
Sbjct:   81 MAQNLIKAGCDVTVWNRTKSKCDPLVGLGAKYKSSPEEVTATCDLTFAMLADPESAIDVACGKNGAIF---------GISSGKGYVDVSTVDVASSILISKQIKDTGALFLEAPVSGSKKPAEDGQLIFLTAGDKPLYEKAAPFLDIMGKSKFYLGEVGNGAAMKLVVNMIMGSMMASFAEGILLSQKVGLDPNVLVEVVSQG-----AINA--PMYSLKGPSMIKSVYPTAFPLKHQQKDMRLALGLAESVSQSTPIAAAANELYKVAKSYGLSDEDFSAVI 347          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. SwissProt
Match: gi|123329297|sp|Q0KBC7.1|LTND_CUPNH (RecName: Full=L-threonate dehydrogenase)

HSP 1 Score: 93.9745 bits (232), Expect = 2.328e-21
Identity = 66/203 (32.51%), Postives = 99/203 (48.77%), Query Frame = 0

Query:   65 GACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEA-GAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEGGKDFSAML 266
            G  ++  +TV    A  + ++ +E GLL LDAPVSGG   AA+G +T M  G   A++  E +L  +  K    G A GAG   KI N +L  + +  + EA+ L  + G+D   L+DV++ S+G  W      P            DY P  A  + +KDLG+    A       PL A A +++      G GG+D SA++
Sbjct:   89 GKLVIASATVPPGFAEALGRRLAEQGLLMLDAPVSGGAARAASGEMTMMTSGPAEAYSLAEDVLAAIAGKVYRLGAAHGAGSKVKIINQLLAGVHIAAAAEAMALGLREGVDPDALYDVITHSAGNSWMFENRVP-------HILKGDYTPLSAVDIFVKDLGMVLDTARHSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVI 284          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. SwissProt
Match: gi|1723179|sp|P44979.1|LTND_HAEIN (RecName: Full=L-threonate dehydrogenase)

HSP 1 Score: 92.0485 bits (227), Expect = 1.506e-20
Identity = 63/203 (31.03%), Postives = 100/203 (49.26%), Query Frame = 0

Query:   65 GACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEA-GAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEGGKDFSAML 266
            G  ++  ST+  Q A+ + Q+ +E GL+ LDAPVSGG   A  G +T MA GS  AF  ++P+L+    K  + GE  G G   KI + +L  + +    EA+ LA K G+    ++DVV+ ++G  W                 + DY P     + +KDLGL    A+ +     L + A  ++    + G G +D SA++
Sbjct:   93 GTAVMVSSTMAAQDAQIISQKLTELGLIMLDAPVSGGAAKALKGEMTVMASGSKQAFELLQPVLDATAAKVYNIGEEIGLGATVKIVHQLLAGVHIAAGAEAMALASKAGIPLDVMYDVVTNAAGNSWMFENRMK-------HVVEGDYTPLSMVDIFVKDLGLVNDTAKSLHFPLHLASTAYSMFTEASNAGYGKEDDSAVI 288          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. SwissProt
Match: gi|75164836|sp|Q949M8.1|3HID3_ARATH (RecName: Full=Probable 3-hydroxyisobutyrate dehydrogenase-like 3, mitochondrial; Short=HIBADH-like)

HSP 1 Score: 90.5077 bits (223), Expect = 6.319e-20
Identity = 78/270 (28.89%), Postives = 130/270 (48.15%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGY-----DVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRS-------VYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEG 258
            M ++++AAG+SV+ Y         +   G +   S   +AE +++V +++ N N +RS       V + L P   T    +D ++     AR++  +A      ++DAPVSGG  GA  GTL   AGG      ++ P++  +G    + GEAG+GQ+ KI N +  A +++G  E I  A+K GLD  K  + V   +     M  +              DY+    A  M+KDLG++ +AA   +A +       +L+  MV +G+G
Sbjct:   49 MVSHIIAAGYSVTVYARDLRKTKDLQTKGARIANSPKELAEMSDVVFTIVGNFNDVRSLLLGDDGVLSGLTPGGVT----VDMTSSKPGLAREIHAEARRRNCWAVDAPVSGGDAGAREGTLGIFAGGDSEIVEWLSPVMKNIGT-VTYMGEAGSGQSCKIGNQIAGASNLVGLAEGIVFAEKAGLDTVKWLEAVKDGAAGSAVMRLFGEM-------IVKRDYRATGFAEYMVKDLGMAAEAAMPGAALSK------QLFTGMVANGDG 300          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. nr
Match: gi|1224843203|ref|WP_092859713.1| (3-hydroxyisobutyrate dehydrogenase [Albimonas pacifica] >gi|1099400960|emb|SFI13335.1| 3-hydroxyisobutyrate dehydrogenase [Albimonas pacifica])

HSP 1 Score: 377.481 bits (968), Expect = 2.681e-129
Identity = 194/273 (71.06%), Postives = 221/273 (80.95%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEGGKDFSAMLLRLMQQA 273
            MAANL  AGH V+G+DVAG T +GV S AS     +G + VI+MLPNG+ILR+V AE+IPA   GA  +DCSTVDV SAR     A+EAGLLS+DAPVSGG+GGAAAGTLTFMAGGS+A+F   +PL ++MGQKAVHCGE GAGQAAKICNNM+L ISMIG CEA NLADKLGL RQ +FDVVSTSSGYCWSMNAYCPAPGVGP SPADNDY+PGFAA LMLKDL LSQQAA+ V A TP+GA A ELY++ V +   GKDFSAML RL + A
Sbjct:   15 MAANLAKAGHEVTGFDVAGTTAEGVTSAASAVEAVQGKDAVITMLPNGDILRTVAAEIIPAMRKGAVFIDCSTVDVDSARAAHDLAAEAGLLSVDAPVSGGIGGAAAGTLTFMAGGSEASFEAAKPLFDIMGQKAVHCGEGGAGQAAKICNNMILGISMIGVCEAFNLADKLGLSRQSVFDVVSTSSGYCWSMNAYCPAPGVGPQSPADNDYKPGFAAELMLKDLRLSQQAADSVGAPTPMGARAMELYEAFVGEDGKGKDFSAMLPRLSKMA 287          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. nr
Match: gi|1213228041|ref|WP_088632550.1| (3-hydroxyisobutyrate dehydrogenase [Phaeobacter sp. 22II1-1F12B] >gi|1212244609|gb|OWU81422.1| 3-hydroxyisobutyrate dehydrogenase [Phaeobacter sp. 22II1-1F12B])

HSP 1 Score: 376.711 bits (966), Expect = 6.605e-129
Identity = 186/273 (68.13%), Postives = 216/273 (79.12%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEG-GKDFSAMLLRLMQQ 272
            MAANL  AGH+V+G+D   V++DGV    +  A  EGA++VI+MLPNG ILRSV  ++IPA   GA L+DCSTVDV SAR V  QA EAGLL++DAPVSGGVGGA  GTLTFMAGGSDA FA  EPL  +MGQKAVHCG AGAGQ+AKICNNM+L ++MI +CEA  LADKLGLDRQK+FDVVSTSSGY WSMNAYCPAPG+GP SPADNDY+PGFAA LMLKDL LSQQAAE   A TP+G  A  LY   V++ +G G+DFSAML R  ++
Sbjct:   15 MAANLAKAGHNVTGFDTVEVSIDGVDIAETATAAVEGADVVITMLPNGAILRSVADQIIPAMRHGATLVDCSTVDVDSARAVAAQAEEAGLLAVDAPVSGGVGGATNGTLTFMAGGSDAGFARAEPLFEIMGQKAVHCGGAGAGQSAKICNNMILGVTMIATCEAFALADKLGLDRQKMFDVVSTSSGYSWSMNAYCPAPGIGPQSPADNDYKPGFAADLMLKDLRLSQQAAESADADTPMGQLAQALYARFVEEEDGKGRDFSAMLPRFEKR 287          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. nr
Match: gi|1274407565|ref|WP_099591384.1| (3-hydroxyisobutyrate dehydrogenase [Amylibacter sp. 4G11] >gi|1273374710|gb|PIB26558.1| 3-hydroxyisobutyrate dehydrogenase [Amylibacter sp. 4G11])

HSP 1 Score: 374.785 bits (961), Expect = 2.984e-128
Identity = 188/272 (69.12%), Postives = 216/272 (79.41%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEG-GKDFSAMLLRLMQ 271
            MA NL  AGH VSG+D AGV +DGV SV +  A   GA++VI+MLPNG ILR+V  ++IP   TGA  +DCSTVDV SAR V +QA+ AGL ++DAPVSGG+GGA  GTLTFMAGG   AFA   PLL +MGQK+VHCGE+GAGQAAKICNNM+L I+MIG+CEA  LADKLGLDRQK+FDVVSTSSGY WSMNAYCPAPGVGP SPADNDY+PGFAA LMLKDL LSQQAAE   AATPLG  AT+LY   V++ +G G DFSAML R  +
Sbjct:   15 MAHNLAVAGHDVSGFDTAGVKIDGVTSVDTAQAAVVGADVVITMLPNGAILRAVADQIIPEMKTGAVFVDCSTVDVDSARAVAEQANAAGLGAIDAPVSGGIGGATNGTLTFMAGGGADAFATAAPLLEIMGQKSVHCGESGAGQAAKICNNMILGITMIGTCEAFALADKLGLDRQKMFDVVSTSSGYSWSMNAYCPAPGVGPDSPADNDYKPGFAAELMLKDLRLSQQAAESADAATPLGQAATDLYAQFVENEDGLGMDFSAMLPRFSK 286          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. nr
Match: gi|1199238612|gb|OUS05344.1| (3-hydroxyisobutyrate dehydrogenase [Rhodobacterales bacterium 52_120_T64])

HSP 1 Score: 371.318 bits (952), Expect = 7.128e-127
Identity = 190/272 (69.85%), Postives = 224/272 (82.35%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEGGKDFSAMLLRLMQQ 272
            MA NLVAAGH+VS +DVAG++V+G  +V + A  A  A++VI+MLPNG ILR+V  E+IPA  +GACL+DCSTVDV+SAR V +Q   AGLLS+DAPVSGG+GGA AGTLTFMAGGS+AAFA   PL ++MGQKAVHCG+AGAGQAAKICNNM+L  +MI +CEA  LADKLGLDRQK+FDV STSSGY WSMNAYCPAPGVGPTSP+DNDY+PGFAA LMLKDL L+QQAAE   A+TPLG HA ++Y + V+DG  G DFSAML  L  Q
Sbjct:   15 MARNLVAAGHAVSVFDVAGISVEGANTVQTAADAATEADVVITMLPNGAILRNVAGEVIPAMKSGACLVDCSTVDVESARTVAEQTRAAGLLSVDAPVSGGIGGAEAGTLTFMAGGSEAAFAVANPLFDIMGQKAVHCGDAGAGQAAKICNNMILGATMIVTCEAFALADKLGLDRQKMFDVASTSSGYSWSMNAYCPAPGVGPTSPSDNDYKPGFAAELMLKDLNLAQQAAEAADASTPLGQHARDMYAAFVEDGGLGMDFSAMLPHLSNQ 286          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. nr
Match: gi|1224338132|ref|WP_092363514.1| (3-hydroxyisobutyrate dehydrogenase [Cribrihabitans marinus] >gi|1094605179|emb|SEJ03441.1| 3-hydroxyisobutyrate dehydrogenase [Cribrihabitans marinus])

HSP 1 Score: 369.777 bits (948), Expect = 2.768e-126
Identity = 191/273 (69.96%), Postives = 221/273 (80.95%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEG-GKDFSAMLLRLMQQ 272
            MAANLV AGH V+G+D AGVTV+ V   AS    A+GAE+VI+MLPNG ILR V AE+IPA A GA L+DCSTVDV SA+ V Q   +AG+L +DAPVSGG+GGAA GTLTFMAGGSD AFA  EPL ++MGQKAVHCGEAGAGQAAKICNNM+L ++MI +CEA  LADKLGLDRQK+FDVVSTSSGY W+MNAYCPAPG+GP SPADNDY+PGFAA LMLKDL LSQQAAE   A TP+GA A  LY   V++ +G G+DFSAML R  ++
Sbjct:   15 MAANLVKAGHEVTGFDTAGVTVEDVTPAASGPEAAKGAEVVITMLPNGAILRLVAAEVIPAMAKGATLVDCSTVDVDSAKSVAQDTEKAGILFVDAPVSGGIGGAAGGTLTFMAGGSDVAFAKAEPLFDIMGQKAVHCGEAGAGQAAKICNNMILGVTMIATCEAFALADKLGLDRQKMFDVVSTSSGYSWTMNAYCPAPGIGPQSPADNDYKPGFAADLMLKDLRLSQQAAESADADTPMGALAQALYARFVEEEDGAGRDFSAMLPRFEKR 287          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. nr
Match: gi|1110746967|ref|WP_071972123.1| (3-hydroxyisobutyrate dehydrogenase [Sulfitobacter sp. AM1-D1] >gi|1108552164|gb|APE43786.1| 3-hydroxyisobutyrate dehydrogenase [Sulfitobacter sp. AM1-D1])

HSP 1 Score: 369.777 bits (948), Expect = 3.790e-126
Identity = 185/270 (68.52%), Postives = 217/270 (80.37%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEG-GKDFSAMLLRL 269
            MAANL  AGH V G+DVAG T +GV+   +I     GA++VI+MLPNG+ILR V AE I     GA  +DCSTVDV+SAR+  + A +AGLL++DAPVSGGVGGA AGTLTFMAGGS+AAFA   PL  +MGQKAVHCG+AGAGQ+AKICNNM+L  +MI +CEA  LADKLGLDRQ++FDVVSTSSGY WSMNAYCPAPGVGP SPADN Y+PGFAA LM+KDLGLSQQAAE V A TP+GA A ELY++ V++ +G GKDFSAML R 
Sbjct:   18 MAANLAKAGHKVRGFDVAGTTAEGVEQAQTIPDAVTGADVVITMLPNGSILRQVAAEAIGHMREGAVFIDCSTVDVESARNTSEAAEKAGLLAVDAPVSGGVGGAEAGTLTFMAGGSEAAFAAAAPLFEIMGQKAVHCGDAGAGQSAKICNNMILGATMIATCEAFALADKLGLDRQRMFDVVSTSSGYSWSMNAYCPAPGVGPKSPADNGYKPGFAAELMVKDLGLSQQAAELVDADTPMGARALELYKAFVEEEDGKGKDFSAMLPRF 287          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. nr
Match: gi|1275798956|ref|WP_099866507.1| (3-hydroxyisobutyrate dehydrogenase [Pararhizobium sp. XC0140])

HSP 1 Score: 368.622 bits (945), Expect = 6.989e-126
Identity = 190/269 (70.63%), Postives = 220/269 (81.78%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEGGKDFSAMLLRL 269
            MA+NLV AGHSV+G+DVAGV VDGV   +S A  A G ++VI+MLPNG+ILRSVYAE++PA   G+C +DCSTVDV SAR   + AS+A LLS+DAPVSGGVGGA AGTLTFMAGGS+AAFA  EPL ++MGQKAVHCG AG+GQAAKICNNM+L ISMIG CEA  LADKLGL RQ +FDVVSTSSG CWSMN YCP PG+GP SPADN Y+PGFAA LMLKDLGL+Q AAE V A TP+GA A  LY++ VD G  G+DFSAM+ +L
Sbjct:   15 MASNLVKAGHSVTGFDVAGVKVDGVADASSAADAARGKDVVITMLPNGDILRSVYAEIVPAGTAGSCFIDCSTVDVDSARAAHEMASKADLLSVDAPVSGGVGGATAGTLTFMAGGSEAAFAIAEPLFSIMGQKAVHCGPAGSGQAAKICNNMILGISMIGVCEAFVLADKLGLSRQSVFDVVSTSSGSCWSMNTYCPVPGIGPQSPADNAYKPGFAAELMLKDLGLAQDAAESVGAPTPMGAKAHALYRAFVDQGGKGRDFSAMIEKL 283          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. nr
Match: gi|1200523258|gb|OUW36357.1| (3-hydroxyisobutyrate dehydrogenase [Gammaproteobacteria bacterium TMED183])

HSP 1 Score: 367.466 bits (942), Expect = 2.377e-125
Identity = 184/274 (67.15%), Postives = 216/274 (78.83%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEG-GKDFSAMLLRLMQQA 273
            MAANLVAAGH V+G+DVA VT+DGV  V   A  A G ++VI+MLP+G ILRSV   ++P   +GA LLDCSTVDV SAR V   A + GLL++DAPVSGG+GGA AGTLTFMAGGS+A FA   PL +VMG+KAV+CG++GAGQAAKICNNM+L I+MIG+CEA  LAD+LGLDRQ++FDVVSTSSGY W MN YCPAPGVGP SPADN YQPGFAA LMLKDL L+Q AA   +A TPLG  A ELY+  V+D +G GKDFSAML R  + A
Sbjct:   15 MAANLVAAGHEVTGFDVAPVTIDGVSMVTDXAQAATGRDIVITMLPDGAILRSVADSILPVMTSGALLLDCSTVDVDSARAVAAMAGDHGLLAVDAPVSGGIGGAEAGTLTFMAGGSEAGFAMASPLFDVMGRKAVYCGDSGAGQAAKICNNMILGITMIGTCEAFALADRLGLDRQRMFDVVSTSSGYSWVMNDYCPAPGVGPKSPADNGYQPGFAAELMLKDLMLAQMAAGATNAETPLGQAALELYRQFVEDEDGRGKDFSAMLPRFEKAA 288          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. nr
Match: gi|746104092|ref|WP_039170125.1| (3-hydroxyisobutyrate dehydrogenase [Leisingera sp. ANG-S5] >gi|742723424|gb|KIC34810.1| 3-hydroxyisobutyrate dehydrogenase [Leisingera sp. ANG-S5])

HSP 1 Score: 367.466 bits (942), Expect = 2.529e-125
Identity = 192/274 (70.07%), Postives = 222/274 (81.02%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEG-GKDFSAMLLRLMQQA 273
            MAANL  AGH V+G+D+A V+++GV+  AS    A GA+ VI+MLPNG ILR V AE+IPA A GA L+DCSTVDV SAR V +QA  AGLLS+DAPVSGG+GGAA GTLTFMAGGS  AFA  EPL ++MGQKAVHCGEAGAGQAAKICNNM+L ++MI +CEA  LADKLGLDRQK+FDVVSTSSGY W+MNAYCPAPGVGP SPADN+YQPGFAA LMLKDLGLSQQAAE   A TP+G  A  LY+  V+D +G G DFSAML R  +++
Sbjct:   15 MAANLAKAGHEVTGFDMADVSIEGVKMAASGPEAAAGADAVITMLPNGAILRLVAAEVIPAMAKGAALIDCSTVDVDSARAVAEQAEAAGLLSVDAPVSGGIGGAAGGTLTFMAGGSAQAFAAAEPLFDIMGQKAVHCGEAGAGQAAKICNNMILGVTMIATCEAFALADKLGLDRQKMFDVVSTSSGYSWTMNAYCPAPGVGPQSPADNNYQPGFAAELMLKDLGLSQQAAESADADTPMGELAMALYKKFVEDEDGKGMDFSAMLPRFEKRS 288          

BLAST of 3-hydroxyisobutyrate dehydrogenase vs. nr
Match: gi|746078678|ref|WP_039146103.1| (3-hydroxyisobutyrate dehydrogenase [Leisingera sp. ANG-DT] >gi|742707677|gb|KIC19533.1| 3-hydroxyisobutyrate dehydrogenase [Leisingera sp. ANG-DT])

HSP 1 Score: 367.466 bits (942), Expect = 3.013e-125
Identity = 192/274 (70.07%), Postives = 221/274 (80.66%), Query Frame = 0

Query:    1 MAANLVAAGHSVSGYDVAGVTVDGVQSVASIAAVAEGAELVISMLPNGNILRSVYAELIPAAATGACLLDCSTVDVQSARDVQQQASEAGLLSLDAPVSGGVGGAAAGTLTFMAGGSDAAFAFVEPLLNVMGQKAVHCGEAGAGQAAKICNNMLLAISMIGSCEAINLADKLGLDRQKLFDVVSTSSGYCWSMNAYCPAPGVGPTSPADNDYQPGFAAALMLKDLGLSQQAAEQVSAATPLGAHATELYQSMVDDGEG-GKDFSAMLLRLMQQA 273
            MAANL  AGH V+G+D+A V+++GV+  AS    A GA+ VI+MLPNG ILR V AE+IPA A GA L+DCSTVDV SAR V +QA  AGLLS+DAPVSGG+GGAA GTLTFMAGGS  AFA  EPL  +MGQKAVHCGEAGAGQAAKICNNM+L ++MI +CEA  LADKLGLDRQK+FDVVSTSSGY W+MNAYCPAPGVGP SPADN+YQPGFAA LMLKDLGLSQQAAE   A TP+G  A  LY+  V+D +G G DFSAML R  +++
Sbjct:   15 MAANLAKAGHEVTGFDMADVSIEGVKMAASGPEAAAGADAVITMLPNGAILRLVAAEMIPAMAKGAALIDCSTVDVDSARAVAEQAEAAGLLSVDAPVSGGIGGAAGGTLTFMAGGSAEAFAAAEPLFGIMGQKAVHCGEAGAGQAAKICNNMILGVTMIATCEAFALADKLGLDRQKMFDVVSTSSGYSWTMNAYCPAPGVGPQSPADNNYQPGFAAELMLKDLGLSQQAAESADADTPMGELAMALYKKFVEDEDGKGMDFSAMLPRFEKRS 288