BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000003059 (supercontig:LSalAtl2s:LSalAtl2s1734:14347:19068:1 gene:EMLSAG00000003059 transcript:EMLSAT00000003059 description:"maker-LSalAtl2s1734-augustus-gene-0.5")

HSP 1 Score: 805.053 bits (2078), Expect = 0.000e+0
Identity = 448/1006 (44.53%), Postives = 625/1006 (62.13%), Query Frame = 0

Query:   38 YVYDADQSFRK-LTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLP-AKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASG--IEAEY----LAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDPAGFESLAKVIHHSFDINMAVAILRLQNGCD--------------YSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSN--------KRVPKTVLLG---SDGKPLPAKLVQEINAFKTKKRV--GFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQAN----------------------------ISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            + +   +S R  LT EA+P   HYR   S      +SS  RPT+ +L +       ++   ++ES  + +D       G VIKFGWI+GV                            GL +I++ N+VT +T++SMSAVSTNG I+ GGIY+MISRSLG EFGGAIG+MFT+ANSIAVA YIIGF ++L DML + +PGF GI +T +  L+D+R IG+V+L  +L LA+VGMEWVTRV+K LL+LL+ SQ+DF+IG+FLP ++ +   GFVGYN     +NL +    +    KN   FF V AVFFPAVTGIVAGANLSGDL+DP +AIPKGTL  +V TY +Y IY  +I +    EASG  +E +Y    +  + N+T+     F  C  R C YG  + QQ +  IS  G L+YAGCFAATLSSAIASLVGAPRV QA+AKD L+P +  F  G+G NNDP++GY++VF I+  CI+IG+L+ VSSLLSNFFVA YALINFSVFHASITKSPGWRP FKYYNKW+SL  T+LC+ VMFLM  VTA++TF+ +  LY+++S RKPE NWGSS  AQ F+T+L ++ +L  V+ HVKNYRP I+++SG P +R  L+DFANL+TK++SLL    V  E +  K ++ + +  ++W  ++ IK FFS+  +    EGA +++ L+GLGKL+ N++++G++  W +     +   K+I  +FD+ +AV +LR+  G D               SN +  +   +   S     D     G      ++ ++++E+   K+  K N        K+ P ++ L     D KPL   +V  ++ F+ K  V  G IDV+WLYDDG       HIL  RA+++ C MRVF L+ KND LD Q+RSMA+LLAKFRIDF DV+M+ D  K+     KDEFK +L   +                            +S+ ++++N EKTN +LR+AE+++++SS ++ +
Sbjct:  100 FQFTTQRSLRHYLTREAIPHADHYRNKMSFAKGKPDSS--RPTLDDLLNDDPYSKDNS---DLESQTDDQD------TGSVIKFGWIEGVYMRCLLNIWGVMLFLRLTWVIGQAGLLEGLAIITLSNIVTVITSISMSAVSTNGQIKAGGIYFMISRSLGPEFGGAIGLMFTLANSIAVAMYIIGFCEALSDMLKQYIPGFIGILNTPEDGLHDIRLIGSVSLVLLLGLAIVGMEWVTRVQKGLLVLLLFSQLDFIIGSFLPPSEDKLAKGFVGYNGTVLMENLFSEYIPNEGTEKNN-SFFTVFAVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLXIVSTYVTYTIYGVMIAAXTXREASGDVLELDYVNETIKEVNNITNS----FWECENRTCSYGILHSQQMMEVISAWGPLIYAGCFAATLSSAIASLVGAPRVFQAVAKDKLFPGIASFADGWGKNNDPVKGYLLVFLISLICILIGDLNYVSSLLSNFFVAAYALINFSVFHASITKSPGWRPGFKYYNKWISLLGTVLCICVMFLMDMVTALVTFIIVFFLYMYVSIRKPEVNWGSSTQAQSFMTALNSVLSLTKVEDHVKNYRPKIMVMSGSPLNRPTLIDFANLVTKRISLLESVDVVPEDLDWKKSQAIKNSGQNWFTENHIKGFFSVVRNSKISEGARTAMELSGLGKLRTNMILLGFQDTWWKKDESAKEYYKIIQTAFDLRLAVGVLRINGGLDVTSMTNLMEDSSPNQSNALNYDDRCESNMSVDSGLDLATKSGAKNTSTQSGEDDKEDGSLKRNKKPNGFEKLLFKKKCPSSIPLPPSEKDVKPLDEDIVNRLSQFRDKTTVNTGTIDVYWLYDDGXXXX---HILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVPPLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLI 1086          

HSP 2 Score: 122.865 bits (307), Expect = 1.969e-28
Identity = 65/160 (40.62%), Postives = 94/160 (58.75%), Query Frame = 0

Query:  954 TKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQAN----------------------------ISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
            T    G IDV+WLYDDG       HIL  RA+++ C MRVF L+ KND LD Q+RSMA+LLAKFRIDF DV+M+ D  K+     KDEFK +L   +                            +S+ ++++N EKTN +LR+AE+++++SS ++ +V+
Sbjct:  932 TTVNTGTIDVYWLYDDGXXXX---HILQTRAKYSKCKMRVFFLSNKNDELDGQTRSMASLLAKFRIDFQDVIMLTDATKQPKKETKDEFKAMLANNSLKNSNSGKKLSTSSRDSSSSECDEVPPLVSELDIIANKEKTNFNLRIAEIVKDNSSSADLIVM 1088          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000009267 (supercontig:LSalAtl2s:LSalAtl2s58:680408:685072:1 gene:EMLSAG00000009267 transcript:EMLSAT00000009267 description:"augustus_masked-LSalAtl2s58-processed-gene-6.1")

HSP 1 Score: 486.878 bits (1252), Expect = 6.927e-156
Identity = 271/482 (56.22%), Postives = 333/482 (69.09%), Query Frame = 0

Query:   37 TYVYDADQSFRK-LTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL--------GLL-------------------VISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRE----CQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMI 486
            TY     +SFR+ LT +ALPSE HYR L SI + P   S  RPT+ EL      + ++   ++ +   +VE    +  KGKV++FGWI+GV         G++                   VI++ NLVT +TTLSMSAVSTNG IRGGGIYYMISRSLG EFGGAIGIMFT+ANSIAV+ YIIGF  SLLDM  +    F+          ND+R IGA++L  VL LA+VGMEWVTRV+ +LL LLI SQ+DFV+G+FLP K +A YGFVGYN   F  NL  T YHDYK+P NPP FF V  VFFPAVTGIVAGANLSGDL+DP +AIPKGTL A+++T+ +YI Y T+I     +EASG + EY  ++  V  +  L+F+NC+ R     CQYGSSNDQQ + +IS TGYL+YAGCFAATLSSAIASLVGAPRVLQALAKD LYP++ +F  GFGANNDP+RGY +VF I+  CI+I
Sbjct:   53 TYSTQYFKSFRQYLTRDALPSESHYRNLLSISNHPRKFS--RPTLEELQE---EQAENGRLLDKKDMDDVEKNEEEQSKGKVVRFGWIEGVFMRCLLNIWGVMLFLRLTWVVGQGGLIQGLGVIALGNLVTVITTLSMSAVSTNGQIRGGGIYYMISRSLGPEFGGAIGIMFTLANSIAVSMYIIGFCQSLLDMCKQY---FELEHLIFSDTTNDVRIIGAISLVAVLALAIVGMEWVTRVQMILLFLLIGSQIDFVVGSFLPTKEDAAYGFVGYNGTLFVDNLSPT-YHDYKDPGNPPSFFSVFGVFFPAVTGIVAGANLSGDLKDPGVAIPKGTLLAIIVTFITYIGYGTIITGVMHTEASGNQTEYENWINGV--EGALYFNNCSGRPADDFCQYGSSNDQQAMERISYTGYLIYAGCFAATLSSAIASLVGAPRVLQALAKDKLYPFIHIFAPGFGANNDPIRGYFLVFIISMGCILI 523          

HSP 2 Score: 406.757 bits (1044), Expect = 1.367e-125
Identity = 193/382 (50.52%), Postives = 260/382 (68.06%), Query Frame = 0

Query:  583 QQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKS---------NKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQAN--ISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            QQFVT+L+++Q+LN   +HVKNYRP +L+ +GLPAHR PLVDFANL+TKKLSLL+CAHV     P K  E + + ++ W  DH+IK+F+S+T ++NF EGA++ +NL G+GK++PN+V+ GYK DW+EDP G +    V+HH+FD+++ V ILRL++GCD+S  I  E   +                        +DEE E+E+  K+ KS          ++V   V  G DG PLP   VQ+I  F+ KKR G IDVWWLYDDGGL+LLLP+ILT RAQFA+C +RVF+LA + D LD ++R+MA LLAKFRI +  V +IPD+ KKA DP K+EFK IL +    + + E++S  EKTNRHLRL ELLREHS  SE +
Sbjct:  539 QQFVTTLKSIQSLNDTPEHVKNYRPKVLVFTGLPAHRPPLVDFANLITKKLSLLMCAHVNIGDAPFKNIELLRNTVQMWFRDHKIKSFYSLTQNKNFEEGAIACMNLAGIGKMRPNMVLFGYKGDWLEDPKGLDEYISVLHHAFDLHLGVGILRLESGCDFSKVIGEEEQQQ--------------------HEIELDEENEDEKNGKQGKSLELQQKKVRXRKVSTAVYTGIDGNPLPKNTVQDITQFQIKKRKGNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKILDEIKPPLEESEIISQKEKTNRHLRLTELLREHSKRSEMI 900          

HSP 3 Score: 177.178 bits (448), Expect = 1.441e-45
Identity = 82/133 (61.65%), Postives = 103/133 (77.44%), Query Frame = 0

Query:  955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQAN--ISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
            KKR G IDVWWLYDDGGL+LLLP+ILT RAQFA+C +RVF+LA + D LD ++R+MA LLAKFRI +  V +IPD+ KKA DP K+EFK IL +    + + E++S  EKTNRHLRL ELLREHS  SE +++
Sbjct:  770 KKRKGNIDVWWLYDDGGLTLLLPYILTTRAQFAECQLRVFALANRKDELDRETRNMAXLLAKFRIXYXXVTVIPDVTKKALDPTKEEFKKILDEIKPPLEESEIISQKEKTNRHLRLTELLREHSKRSEMIIM 902          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000003697 (supercontig:LSalAtl2s:LSalAtl2s1:1949199:1954526:1 gene:EMLSAG00000003697 transcript:EMLSAT00000003697 description:"maker-LSalAtl2s1-augustus-gene-19.17")

HSP 1 Score: 253.062 bits (645), Expect = 6.720e-71
Identity = 175/613 (28.55%), Postives = 300/613 (48.94%), Query Frame = 0

Query:  118 IKFGWIDGVLG----LLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCV----LILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKY------GFVG-----YNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGK--GFG-ANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPV-TAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLD--DH-RIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGY 704
            ++ G+I G  G    LL I +   +   T LS+SA++TNG ++GGG+Y+MISR+LG EFGG+IG++F  AN  + A Y +   + ++    E        + T+ H L +  W   +    +    L + ++G     +   ++L+ ++   +  +   F  +  E  +       F G     ++ E F  +L  +   D  +   P  F  V AV F  VTGI+AGANLSGDL++P+ +IPKGTL A   T+ +Y+I  TL        A   + E L                  +R+C Y        + + S     V  G   AT  ++ + L+G+ RVL+A++KD ++   G+F +   +G   N+P+   VV + ++     IG ++ ++  ++  F+  YA +N S     +  +P +RP F+ ++ +      I  + +MF+++PV +A++ F CI            + NWGS   A  F    + L  L++ + HVK +RP +LLL   P +   L+DFAN L KK  L +  HV + +      ++    + HWL+  DH ++KAF  +T +     G    I ++G+G +KPN ++ G+
Sbjct:   44 LRVGYILGNAGILHSLLQIVIAYGILVFTILSVSAIATNGAVKGGGVYFMISRTLGPEFGGSIGVLFYAANIASSALYAVACTECIISSFGEE-------SGTLAHYLPESFWYQILYRVLINLLNLSICLIGPNLFGKTTIMVLISVVFCTLTVITSFFSASTVEVHFNETISGSFTGILSKNWSIENFQSHLYPSYSLDCNDDTKPVNFAIVFAVLFSGVTGIMAGANLSGDLKEPSKSIPKGTLLASFFTFITYVILFTL-------TAMTCDKELL------------------LRDCSY--------MIEFSFWKPFVIIGTVLATFCASTSCLIGSSRVLEAISKDTMF---GIFLEWITYGTVGNNPVLAVVVTWLLSNIFFFIGGVNTIAQFVAVLFLLSYASVNLSCLSLDLASAPNFRPIFRCFSWYTCALGLIGTLTMMFMINPVISALVLFACIFFFLSLSFLSPTKENWGSISQALLFHQVRKYLLLLDSRKDHVKFWRPQVLLLVSNPRNHCSLIDFANAL-KKGGLYVLGHVREGNFEGMEEDECGLLMPHWLNLVDHLKVKAFVELTVAPTVRSGIQQLIRISGIGAMKPNTIIFGF 612          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000004234 (supercontig:LSalAtl2s:LSalAtl2s222:1210791:1213007:1 gene:EMLSAG00000004234 transcript:EMLSAT00000004234 description:"maker-LSalAtl2s222-snap-gene-12.7")

HSP 1 Score: 221.09 bits (562), Expect = 1.084e-61
Identity = 166/606 (27.39%), Postives = 281/606 (46.37%), Query Frame = 0

Query:  147 SAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLS--ENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQV--------------DFVIGTFLPAK------------------SEAKYGFVGYNTET-----FTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLY-PYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKP--EANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWL---DDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSD 707
            SA+S+NG + GGG+Y+M+SR+LG EFGGAIG +F  AN ++ A Y+ G  + L+         P  + +  T  + LN             L + +VG ++  +   L+  +++V  +              DF+     P K                  S  K+  +G   ET     F  NL     +D         FF +  V F  VTGI++GAN+ G+L  P+ +IP+GT++A   T   YII                    L  L ++T    L        +C Y        ++ ++V    V  G F AT S+++ +L+G  R+L+A+AKD ++ P      KG    N P+   +  +F+    +M+G+L+ ++ L S  F+  Y  IN +     +  +P +RP++KY++ +  L   +  + +MF +S + + +  +    L L ++   P    NWGS   A  F    + L  L+  + HVK +RP +LLL   P     L++F N L KK  L +  HV  +++ +   +   + I HWL   D  ++KAF  +T +++   G    + ++G+G +KPN V++G+K D
Sbjct:   16 SALSSNGAVEGGGVYFMLSRTLGPEFGGAIGTLFFFANVVSSALYLAGCTEGLVSNFKFLARGPWIEFLYSTSINSLN-------------LFICLVGSKFFGKTSVLIFFMVLVCVLMTSVSFFLDYTITEDFIFNATDPLKCVQPSKNDTNQTPNCTLTSTGKFTGIGSWNETILVQNFYDNLYPKYEYDCSGSDKSLNFFIIFGVLFSGVTGIMSGANMCGELVSPSKSIPRGTISACGFTLVIYII--------------------LTVLTSLTCSPTLL-----QHDCMY--------MNIVNVLPPAVTIGVFMATFSASLNNLIGXSRILEAIAKDVVFGPLFNFVLKGTYKEN-PVTAVISTWFVVELFLMMGSLNNIAQLCSVLFLLSYGTINLTCLFLHLASAPNFRPTYKYFSTYTCLIGLLGSIIMMFAVSAIYSAVGILLCLSLILLLNLFSPIRHTNWGSISQALIFHQVRKYLLLLDQRKSHVKFWRPQMLLLVSNPRRNCSLIEFGNAL-KKSGLYVLGHVHVDNLEEHDKDPTLNEIPHWLKLIDFLKVKAFVEVTIARSLRLGVDQLVRISGIGAMKPNTVLLGFKDD 573          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. L. salmonis genes
Match: EMLSAG00000007778 (supercontig:LSalAtl2s:LSalAtl2s455:327857:377250:-1 gene:EMLSAG00000007778 transcript:EMLSAT00000007778 description:"maker-LSalAtl2s455-augustus-gene-2.20")

HSP 1 Score: 193.356 bits (490), Expect = 1.968e-50
Identity = 127/453 (28.04%), Postives = 210/453 (46.36%), Query Frame = 0

Query:  309 FFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYI----IYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLL----SGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSH--IEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWM-EDPAGFESLAKVIHHSFDINMAVAILR-LQNGCDYSNKIKS 749
            F  ++ +FFP+ TGI+AG+N SGDL  P   IP GT+ A++ T   Y+    ++A  +    L +  G        +GN+                                   +V  G   +T+ + + SL GAPR+LQA+AKD + P+L  F +   A  +P+R   +   I    ++IGN+D ++ LLS FF+  Y  +N +    ++ ++P WRP FKYY+  +SL   +LC+AVMF+ S   A++      I+Y +I YR  E  WG          +  +L  L     H KN+RP IL+L    S L      L   A+ L     L I   V      +   E  ++   I+  + D  +K F  +  S++  +G       TGLG +KPN V++G+ + W  ++   ++     + ++    MA+ + + +Q   D  +K+K 
Sbjct:  449 FVILVGIFFPSCTGIMAGSNRSGDLAXPQKXIPIGTIMAILTTSTVYLSSVLLFAATVDPLLLRDKFGESIGGSLVVGNIAWPHP-----------------------------KVVEIGSLLSTIGAGLQSLTGAPRLLQAIAKDGIIPFLAPF-QVQSAKGEPVRALGITLLICEVAVLIGNVDEIAPLLSMFFLMCYGFVNLAXALQTLLRTPNWRPRFKYYHWSLSLLGVVLCIAVMFMSSWYYALLAMGIAGIIYKYIEYRGAEKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILAKLDSQLKVKHKRLFALASQLKAGKGLTIGVTVLDGHFGKMYGEAQAAKFSIQQVIKDEAVKGFADVVVSKDISDGVCHLTQTTGLGGMKPNTVILGWPNSWRKKEEDSWKVFVDSVRYASAAKMALLVPKGIQRYPDSGDKVKG 871          

HSP 2 Score: 80.8777 bits (198), Expect = 1.179e-15
Identity = 59/178 (33.15%), Postives = 86/178 (48.31%), Query Frame = 0

Query:  118 IKFGWIDG----VLGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVA-----------TYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKYGF 280
            I+  WI G    V G  V+ +   VT LT +SM+ ++TNGV+ GGG  +MISR+LG EFGGA+GI F  A  +A A            YI+G   SL         G D   D V  + N+ R  G   L  +  +  +G+ +V +   + L  +++S V   +G F+      K  F
Sbjct:  168 IRMTWIVGTAGAVEGFFVVFMGCCVTLLTAISMAXIATNGVVPGGGSXFMISRALGPEFGGAVGIXFYXAXXVAAAMYIIGAIEILIIYIVGPAMSLF--------GPDITNDFV--KFNNYRVYGTFLLITMGTIVFIGVRFVNKFAAVALACVLLSIVSIYVGIFVNIGGNDKANF 335          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709294|sp|P55013.1|S12A2_SQUAC (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; AltName: Full=NKCC; AltName: Full=Na-K-CL symporter)

HSP 1 Score: 654.055 bits (1686), Expect = 0.000e+0
Identity = 386/1031 (37.44%), Postives = 587/1031 (56.94%), Query Frame = 0

Query:   13 KSEKGEPPNGGFYQSTSISENNMNT------YVYDAD------QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPK--TPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASG-----IEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEH--WLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDW----MEDPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSN----------KIKSEH----LVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPL---PAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL---------------------TQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            +S  G P NG     T +SE ++++      + YD        ++F   T++A+P   HYR   + + +       RP++ EL      E     ++  E     E+   K       V+KFGWI GVL                            LLVI    +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGGAIG++F  AN++AVA Y++GF +++ D+L E+       A  +D  ++D+R IG+VT+  +  ++V GMEW  + + +LL +L+++ V+F +GTF+PA  +   GF  Y  E F++N +     D+++ ++   FF V A+FFPA TGI+AGAN+SGDL DP +AIPKGTL A+++T   Y   A  +GSC + EA+G     I    +    NV  K G  F +C   +C YG  ND Q +S +S  G L+ AG F+ATLSSA+ASLV AP++ QAL KDN+YP L VF  G+G NN+PLRGYV+ FFI    I+I  L+V++ ++SNFF+A YALINFSVFHAS+ KSPGWRP+F++YN W+SL   ILC  VMF+++   A++T V +  LY++++Y+KP+ NWGSS  A  ++ +L++   L  V+ HVKN+RP  LL++G P  R  L+   +  TK + L++C HV      Q + E  +   ++  WL  +++KAF++  ++++  EG    +   GLG+++PN ++ G+K DW    M+D    E+    IH +FD    V ++RL+ G + S+          + KS H    +V +  SDADS        V E  + A+ +++++EE+  K+ +   + K V       PL     KL+Q  + F+ K+  G IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF +  K + +D   R+MA LL+KFRIDF D+ ++ D+  K S      F+ ++                         I+D E+     KT R +RL ELLRE+S  +  +
Sbjct:  144 ESMNGYPQNG----DTMMSEGSLHSSGTGAHHYYDTHTNTYYLRTFGHNTIDAVPRIDHYRHTVAQLGE----KLIRPSLAELHDELDKEPFEDGYVNGEESSPAEEAVSKHVADNKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGHAGIGLALLVIGTATVVTTITGLSTSAITTNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVRDLLVEHN------ALMID-EMSDIRIIGSVTIVVLFGISVAGMEWEAKAQIVLLGILLLAIVNFTVGTFIPANDKRAKGFFNYRGEIFSENFVP----DFRDGED---FFSVFAIFFPAATGILAGANISGDLADPQLAIPKGTLLAILITTIVYAGAAVSVGSCIVREATGNLTDAIIPGTVTNCTNVACKLGFNFSSCATNKCSYGLMNDFQVMSLVSGFGPLITAGIFSATLSSALASLVSAPKIFQALCKDNIYPGLHVFSVGYGKNNEPLRGYVLTFFIGLGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFRFYNMWISLIGAILCCGVMFVINWWAALLTNVIVLALYIYVTYKKPDVNWGSSTQALTYLNALQHAIRLTGVEDHVKNFRPQCLLMTGAPTSRPALLHLVHAFTKNVGLVVCGHVHTGPRRQALKEISTDQAKYQRWLIKNKMKAFYAPVYAEDLREGTQFLLQAVGLGRMRPNTLVFGFKKDWRQALMKD---VENYINAIHDAFDYQYGVVVIRLKEGFNISHLQAQEELCTSQEKSAHPKDIVVNLEHSDADSSKPSSKS-VSETNSPAVCQDQKDEEDDGKASTQPLLKKEV--KDPSVPLNMTDQKLLQASSQFQKKQGKGTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMIEPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLI 1145          

HSP 2 Score: 103.605 bits (257), Expect = 4.618e-21
Identity = 66/210 (31.43%), Postives = 106/210 (50.48%), Query Frame = 0

Query:  899 DIAKKASDPI-KDEFKTILTQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL---------------------TQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
            D  K ++ P+ K E K      N++D+++L                    + S+   K+  G IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF +  K + +D   R+MA LL+KFRIDF D+ ++ D+  K S      F+ ++                         I+D E+     KT R +RL ELLRE+S  +  +V++
Sbjct:  959 DDGKASTQPLLKKEVKDPSVPLNMTDQKLLQ-------------------ASSQFQKKQGKGTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRTMATLLSKFRIDFSDITVLGDMNTKPSKDNITAFEEMIEPFRLHEDDKEQEASEKMKEEEPWRITDNELEIYRMKTYRQIRLNELLRENSGTANLIVMS 1148          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709292|sp|P55011.1|S12A2_HUMAN (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1)

HSP 1 Score: 650.973 bits (1678), Expect = 0.000e+0
Identity = 380/992 (38.31%), Postives = 570/992 (57.46%), Query Frame = 0

Query:   32 ENNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPS-PKTPKGK-VIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASG-IEAEYLAYLGNVTS---KSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQM--SSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQV--------DLGVLEDIAEAIDEEEEEEEEKKKS-----KSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQA---------------------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            + + NTY     ++F   TM+A+P   HYR  A+ + +       RP++ EL      E     F   E      D     T + K V+KFGWI GVL                            +LVI +  +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGGAIG++F  AN++AVA Y++GF ++++++L E+       +  +   +ND+R IGA+T+  +L ++V GMEW  + + +LL++L+++  DFVIGTF+P +S+   GF GY +E F +N       D++  +    FF V A+FFPA TGI+AGAN+SGDL DP  AIPKGTL A+++T   Y+  A  +GSC + +A+G +    +  L N TS   K    F +C    C YG  N+ Q +S +S    L+ AG F+ATLSSA+ASLV AP++ QAL KDN+YP   +F KG+G NN+PLRGY++ F IA   I+I  L+V++ ++SNFF+A YALINFSVFHAS+ KSPGWRP+FKYYN W+SL   ILC  VMF+++   A++T+V +  LY++++Y+KP+ NWGSS  A  ++ +L++   L+ V+ HVKN+RP  L+++G P  R  L+   +  TK + L+IC HV      Q M E     +  + WL  +++KAF++  H+ +  EGA   +   GLG++KPN +++G+K DW++ D    +    + H +FDI   V ++RL+ G D S+    E L+        + D  V        DL   + ++E     + EEE+ K +     K   + P   L  +D      KL++    F+ K+    IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF +  K + +D   R+MA LL+KFRIDF D++++ DI  K        F+ I+                         I+D E+     KT R +RL ELL+EHSS +  +
Sbjct:  202 DTHTNTYYL---RTFGHNTMDAVPRIDHYRHTAAQLGE----KLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVTYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVLVIMMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEH-------SILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIGDFVIGTFIPLESKKPKGFFGYKSEIFNENFGP----DFREEET---FFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTLVYVGIAVSVGSCVVRDATGNVNDTIVTELTNCTSAACKLNFDFSSCESSPCSYGLMNNFQVMSMVSGFTPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLLGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLNALQHSIRLSGVEDHVKNFRPQCLVMTGAPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQAKYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQYGVVVIRLKEGLDISHLQGQEELLSSQEKSPGTKDVVVSVEYSKKSDLDTSKPLSEKPITHKVEEEDGKTATQPLLKKESKGPIVPLNVAD-----QKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANII 1166          

HSP 2 Score: 105.916 bits (263), Expect = 9.026e-22
Identity = 68/210 (32.38%), Postives = 108/210 (51.43%), Query Frame = 0

Query:  901 AKKASDPI-KDEFKTILTQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVTKKRVG--FIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQA---------------------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
             K A+ P+ K E K  +   N++D+++L                     ++ T  +K+ G   IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF +  K + +D   R+MA LL+KFRIDF D++++ DI  K        F+ I+                         I+D E+     KT R +RL ELL+EHSS +  +V++
Sbjct:  982 GKTATQPLLKKESKGPIVPLNVADQKLL---------------------EASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFEEIIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMS 1169          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|341941994|sp|P55012.2|S12A2_MOUSE (RecName: Full=Solute carrier family 12 member 2; AltName: Full=Basolateral Na-K-Cl symporter; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1)

HSP 1 Score: 650.588 bits (1677), Expect = 0.000e+0
Identity = 382/991 (38.55%), Postives = 578/991 (58.32%), Query Frame = 0

Query:   32 ENNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPS-PKTPKGK-VIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASG-IEAEYLAYLGNVTS---KSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQM--SSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEE-----EEEEEKKKSKSNKRVPKTVLLGSDGK----PL---PAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKAS-----------DPIK----DEFKTILTQAN------ISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            + + NTY     ++F   TM+A+P   HYR  A+ + +       RP++ EL      E     F   E      D     T + K V+KFGWI GVL                            ++VI++  +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGGAIG++F  AN++AVA Y++GF ++++++L E+       +  +   +ND+R IGA+T+  +L ++V GMEW  + + +LL++L+++  DFVIGTF+  +S+   GF GY +E F +N       D++  +    FF V A+FFPA TGI+AGAN+SGDL DP  AIPKGTL A+++T   YI  A  +GSC + +A+G +       L N TS   K    F  C    C YG  N+ Q +S +S    L+ AG F+ATLSSA+ASLV AP++ QAL KDN+YP   +F KG+G NN+PLRGY++ F IA   I+I  L+V++ ++SNFF+A YALINFSVFHAS+ KSPGWRP+FKYYN W+SL   ILC  VMF+++   A++T+V +  LY++++Y+KP+ NWGSS  A  ++++L++   L+ V+ HVKN+RP  L+++G P  R  L+   +  TK + L+IC HV      Q M E     +  + WL  +++KAF++  H+ +  EGA   +   GLG++KPN +++G+K DW++ D    +    + H +FDI   V ++RL+ G D S+    E L+    S   SP  + D+ V  D ++  D++      E+   +K  + + + P   LL  + K    PL     KL++    F+ K+    IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF +  K + +D   R+MA LL+KFRIDF D++++ DI  K             +P +    D+ + I  +        I+D E+     KT R +RL ELL+EHSS +  +
Sbjct:  195 DTHTNTYYL---RTFGHNTMDAVPRIDHYRHTAAQLGE----KLLRPSLAELHDELEKEPFEDGFANGEESTPTRDAVVAYTAESKGVVKFGWIKGVLVRCMLNIWGVMLFIRLSWIVGQAGIGLSVVVIAMATVVTTITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGAIGLIFAFANAVAVAMYVVGFAETVVELLKEH-------SILMIDEINDIRIIGAITVVILLGISVAGMEWEAKAQIVLLVILLLAIADFVIGTFISLESKKPKGFFGYKSEIFNENFGP----DFREEET---FFSVFAIFFPAATGILAGANISGDLADPQSAIPKGTLLAILITTVVYIGIAVSVGSCVVRDATGNVNDTITTELTNCTSAACKLNFDFSYCESNTCSYGLMNNFQVMSMVSGFAPLISAGIFSATLSSALASLVSAPKIFQALCKDNIYPAFQMFAKGYGKNNEPLRGYILTFLIALGFILIAELNVIAPIISNFFLASYALINFSVFHASLAKSPGWRPAFKYYNMWISLIGAILCCIVMFVINWWAALLTYVIVLGLYIYVTYKKPDVNWGSSTQALTYLSALQHSIRLSGVEDHVKNFRPQCLVMTGSPNSRPALLHLVHDFTKNVGLMICGHVHMGPRRQAMKEMSIDQARYQRWLIKNKMKAFYAPVHADDLREGAQYLMQAAGLGRMKPNTLVLGFKKDWLQADMRDVDMYINLFHDAFDIQFGVVVIRLKEGLDISHLQGQEELLS---SQEKSPGTK-DVVVNVDYSKKSDQDTCKSSGEKSITQKDEEEDGKTPTQPLLKKESKGPIVPLNVADQKLLEASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANII 1159          

HSP 2 Score: 103.605 bits (257), Expect = 4.344e-21
Identity = 68/210 (32.38%), Postives = 111/210 (52.86%), Query Frame = 0

Query:  901 AKKASDPI-KDEFKTILTQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVTKKRVG--FIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKAS-----------DPIK----DEFKTILTQAN------ISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
             K  + P+ K E K  +   N++D+++L                     ++ T  +K+ G   IDVWWL+DDGGL+LL+P++LT + ++ DC +RVF +  K + +D   R+MA LL+KFRIDF D++++ DI  K             +P +    D+ + I  +        I+D E+     KT R +RL ELL+EHSS +  +V++
Sbjct:  975 GKTPTQPLLKKESKGPIVPLNVADQKLL---------------------EASTQFQKKQGKNTIDVWWLFDDGGLTLLIPYLLTTKKKWKDCKIRVF-IGGKINRIDHDRRAMATLLSKFRIDFSDIMVLGDINTKPKKENIIAFDDMIEPYRLHEDDKEQDIADKMKEDEPWRITDNELELYKTKTYRQIRLNELLKEHSSTANIIVMS 1162          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|2500792|sp|Q25479.1|NKCL_MANSE (RecName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter; AltName: Full=Na-K-CL symporter)

HSP 1 Score: 631.328 bits (1627), Expect = 0.000e+0
Identity = 378/1023 (36.95%), Postives = 575/1023 (56.21%), Query Frame = 0

Query:    5 SLDNVAFQKSEKGEPPNGGFYQSTSISENNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKV-IKFGWIDGV---------------------------LGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAK--SEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRE-CQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDPAG-FESLAKVIHHSFDINMAVAILRLQNGCDYSN-----------------------KIKSEHLVKIPFSDADSPDAQVD------LGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ-------------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            S++NV     E+G  PN       S SE   +   +   +S  +LT EALP   +YR         +  + +RP++GEL   H         +  E  K+      K+P   V IK GWI GV                           L L++I++  +V  +TTLSMSA+ TNG ++GGGIYY+ISRSLG EFG ++GI+F  AN++A +   IGF DSL D+L  N     G+  T D  +ND+R +G V L  + I+  +GM+W ++ +  L+ +++ + VDFV+GT +  K  SE   GFVG ++ TF +N  +      K  +N   FF V A+FFP+VTGI AGAN+SGDL+DPA AIPKGTL A++++  SY +     G   L +ASG   + L   G VT  S +    C +   C+YG  N    +  +S  G  +Y GC+AATLS+A+ +L+  PR++QAL  D +YP L  F K +G + +P RGYV+ FF++   ++I +L+ ++ L+SNF++A YALINF  FH ++ +  GWRP+F+YYN W+SL   ++CVA+M L+  V +++TF     LYL + YR+P+ NWGSS  AQ + T+L +  AL    +HVKNY P +L+L+G P  R  LVD  NL++K  SL+I   + +E +  K+    +   + WL   +++AF S  H  +F  GA + +  +G+G+L PN+++MGYKSDW   PA    S   V+H +F+  +AVAI+R+  G DYS                        +++ + L+    SD D  D Q        L +    +  I E +E++++KK+ K N    + V   + G  L    + +++ F+ K+  G +DVWWLYDDGGL++LLP+I+++R+ +A+C +R+F+LA +   ++++ R+MANLLAKFRID+  + M+ DI    +DP + E K +  +               ISD E+ +   KTNR LRL ELL  +S D+  V
Sbjct:   35 SVENV-----ERGVAPN-------SQSEGWHHESGWKRRRSLAQLTREALPRMENYRN--------SKRALKRPSLGELHGDH--------LITEEDEKDQNHRDTKSPTPAVGIKLGWIQGVFIPCLLNIWGVMLFLRLSWVVSQAGIGLSLVIIAISAIVCVITTLSMSAICTNGEVKGGGIYYIISRSLGPEFGASVGIIFAFANAVAASMNTIGFCDSLNDLLRSN-----GLKITEDP-INDVRIVGTVALLVMCIICAIGMDWESKAQNFLIAIIVGAMVDFVVGTIMGPKDNSEIAKGFVGLSSATFVENFKSDFRFSEKLDQN---FFSVFAIFFPSVTGIQAGANISGDLKDPASAIPKGTLLALLISMVSYTLMVLFAGGGALRDASGNITDLLIVNGTVTDYSSVSL--CALNNTCEYGLHNSYSVMQLMSAWGPFIYGGCWAATLSTALTNLLSVPRLIQALGVDRIYPGLIFFSKPYGRHGEPYRGYVLTFFVSLLFLLIADLNTIAPLISNFYLASYALINFCTFHRALVRPLGWRPTFRYYNMWLSLAGFLMCVAIMLLVHWVMSLVTFAIFFTLYLIVHYRRPDVNWGSSTQAQMYKTALSSAHALARTGEHVKNYWPQLLVLAGRPQARPALVDLGNLISKAGSLMIVGDISQEKLSYKVRSARARSDDEWLRGRKVRAFCSRVHGFSFEPGARALVQGSGVGRLAPNVLLMGYKSDWTTCPANDLVSYFNVLHTAFENRLAVAIVRVSGGLDYSAVVSEGAEEGAAGSLTATSSSGELRVRRDGLIMHADSDLDIRDTQPKHNLSNLLTLTTSRSFTISECKEKDKKKKERKPNDMHRQIVYNTASGLELSKFQLAQMSLFQKKQESGTLDVWWLYDDGGLTILLPYIISQRSAWANCKLRIFALANRLHEMELEERNMANLLAKFRIDYSSLTMVQDI----TDPPQPETKALFDETIKKFTEESASPDCRISDMELQTLAVKTNRQLRLRELLLANSKDARLV 1014          

HSP 2 Score: 108.227 bits (269), Expect = 1.672e-22
Identity = 61/145 (42.07%), Postives = 94/145 (64.83%), Query Frame = 0

Query:  955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ-------------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
            K+  G +DVWWLYDDGGL++LLP+I+++R+ +A+C +R+F+LA +   ++++ R+MANLLAKFRID+  + M+ DI    +DP + E K +  +               ISD E+ +   KTNR LRL ELL  +S D+  VV++
Sbjct:  877 KQESGTLDVWWLYDDGGLTILLPYIISQRSAWANCKLRIFALANRLHEMELEERNMANLLAKFRIDYSSLTMVQDI----TDPPQPETKALFDETIKKFTEESASPDCRISDMELQTLAVKTNRQLRLRELLLANSKDARLVVMS 1017          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709297|sp|P55016.1|S12A1_RAT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 626.32 bits (1614), Expect = 0.000e+0
Identity = 380/1011 (37.59%), Postives = 570/1011 (56.38%), Query Frame = 0

Query:   24 FYQSTSISENNMNTYVYDAD------QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGEL----------ASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVLGLLVISVCNL---------------------------VTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK-YGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKS----GLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMA-EQMSSHI---EHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDP-AGFESLAKVIHHSFDINMAVAILRLQNGCDYS---------NKIKSEHL-VKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHS 947
            F Q+   ++ +   + YD+       Q+F   TM+A+P   +YR   S+     N    RP++ E+          A G      +   M    G E  +   +   G V+KFGW+ GVL   ++++  +                           VT LT +SMSA+ TNGV+RGGG YY+ISRSLG EFGG+IG++F  AN++ VA Y++GF ++++D+L E+    D +   VD   ND+R IG++T+  +L ++V GMEW  + + +LL++L++   +F IGT +P+ +E K  GF  Y    F +N   +            GFF V A+FFPA TGI+AGAN+SGDL DP  AIP+GT+ A+ +T  +YI  A  + +C + +A+G   + +    N    +    G  F  C    CQYG  N+ Q +S +S  G L+ AG F+ATLSSA+ASLV AP+V QAL KDN++  L  F KG+G NN+PLRGY + F IA A I+I  L+V++ ++SNFF+A YALINFS FHAS  KSPGWRP++  YN WVSLF  ILC AVMF+++   AVIT+V    LY++++Y+KP+ NWGSS  A  +V++L N   L TV+ HVKN+RP  ++L+G P  R  L+D  +  TK   L IC  V     P+K+  ++M+S +   + WL  ++IKAF++   +  F +G  S +  +GLG++KPN +++GYK +W + P +  E+   +IH +FD  + V I+R+  G D S          K++ E L ++    D D  + +  +  L   A  ++      +   K  SN    +++ +G        KLV+    FK K+  G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ +  K + ++ +  SMA+LL+KFRI F D+ +I DI  K   P K+ +K                 LT A          I+D E+ +  EK+ R +RL ELL+EHS
Sbjct:   69 FLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVN----RPSLQEIHEQLAKNVAVAPGSADRVANGDGM---PGDEQAENKEEDVTG-VVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFRFANAVRVAMYVVGFAETVVDLLKES----DSM--MVDPT-NDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIGIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTEG-------EGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVRACVVRDATGSMNDTVVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVG--PRKLCVKEMNSGMAKKQAWLMKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQVQDELEKLEQERLALEAAIKDNDCEEGKGGIRGLFKKAGKLN----ITKPAPKKDSNISTIQSMHVGE----FNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIK---PNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHS 1043          

HSP 2 Score: 101.293 bits (251), Expect = 1.978e-20
Identity = 60/156 (38.46%), Postives = 91/156 (58.33%), Query Frame = 0

Query:  955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
            K+  G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ +  K + ++ +  SMA+LL+KFRI F D+ +I DI  K   P K+ +K                 LT A          I+D E+ +  EK+ R +RL ELL+EHS  +  +V++
Sbjct:  901 KQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKISMASLLSKFRIKFADIHIIGDINIK---PNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLS 1052          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|212276464|sp|Q13621.2|S12A1_HUMAN (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 616.305 bits (1588), Expect = 0.000e+0
Identity = 383/1020 (37.55%), Postives = 575/1020 (56.37%), Query Frame = 0

Query:   24 FYQSTSISENNMNTYVYDAD------QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFM-----EVESGKEV----EDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK-YGFVGYNTETFTKNLLTTNYHDYKNPK--NPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKS----GLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMA-EQMSSHI---EHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDP-AGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEE------KKKSKSN--KRVPKTVLLGSDGK----------PLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHS 947
            F QS   ++ + + + YD+       Q+F   TM+A+P   +YR   SI     N    RP++ E+   H    K+ +        V +G  +    +  + +  +  V+KFGW+ GVL                           G+L+I +  +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGG+IG++F  AN++AVA Y++GF ++++D+L E+    D +   VD   ND+R IG++T+  +L ++V GMEW  + + +LL++L+++  +F IGT +P+ +E K  GF  Y    F +N           P+     GFF V A+FFPA TGI+AGAN+SGDL DP  AIP+GT+ A+ +T  +Y+  A  +G+C + +A+G   + +    N    +    G  F  C    CQYG  N+ Q +S +S  G L+ AG F+ATLSSA+ASLV AP+V QAL KDN+Y  L  F KG+G NN+PLRGY++ F IA A I+I  L+ ++ ++SNFF+A YALINFS FHAS  KSPGWRP++  YN WVSLF  +LC AVMF+++   AVIT+V    LY++++ +KP+ NWGSS  A  +V++L N   L TV+ HVKN+RP  ++L+G P  R  L+D  +  TK   L IC  V     P+K+  ++M+S +   + WL  ++IKAF++   +  F +G  S +  +GLG++KPN +++GYK +W + P    E+   +IH +FD  + V I+R+  G D S  ++ +  ++    +  + +A +            D E EEE        KK  K N  K  PK      DG               KLV+    FK K+  G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ +  K + ++ +   MA+LL+KFRI F D+ +I DI  +   P K+ +K                 LT A          I+D E+ +  EK+ R +RL ELL+EHS
Sbjct:   73 FLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVN----RPSLLEI---HEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKES----DSM--MVDPT-NDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENF---------GPRFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYLGVAICVGACVVRDATGNMNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFLIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYVYVTCKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVG--PRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELERLEQERLALEATIK-----------DNECEEESGGIRGLFKKAGKLNITKTTPK-----KDGSINTSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIR---PNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHS 1047          

HSP 2 Score: 99.3673 bits (246), Expect = 9.919e-20
Identity = 62/179 (34.64%), Postives = 100/179 (55.87%), Query Frame = 0

Query:  933 TNRHLRLAELLREHSSDSETVTKKR-VGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
            T++ + + E  ++    S    KK+  G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ +  K + ++ +   MA+LL+KFRI F D+ +I DI  +   P K+ +K                 LT A          I+D E+ +  EK+ R +RL ELL+EHS  +  +V++
Sbjct:  882 TSQSMHVGEFNQKLVEASTQFKKKQEKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIVMASLLSKFRIKFADIHIIGDINIR---PNKESWKVFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLS 1056          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|27151793|sp|P55014.2|S12A1_MOUSE (RecName: Full=Solute carrier family 12 member 1; AltName: Full=BSC1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 614.379 bits (1583), Expect = 0.000e+0
Identity = 387/1023 (37.83%), Postives = 572/1023 (55.91%), Query Frame = 0

Query:   24 FYQSTSISENNMNTYVYDAD------QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVED---PSPKTPKGK------VIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK-YGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKS----GLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMA-EQMSSHI---EHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDP-AGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLED----IAEAIDEEEEEEEE-------KKKSKSN--KRVPKTVLLGSDGK----------PLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHS 947
            F Q+   ++ +   + YD+       Q+F   TM+A+P   +YR   S+     N    RP++ E+   H    K+ +     + +       P  +  + K      V+KFGW+ GVL                           G+L+I +  +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGG+IG++F  AN++AVA Y++GF ++++D+L E+    D +   VD   ND+R IG++T+  +L ++V GMEW  + + +LL++L+++  +F IGT +P+ +E K  GF  Y    F +N   +            GFF V A+FFPA TGI+AGAN+SGDL DP  AIP+GT+ A+ +T  +YI  A  + +C + +A+G   + +    N    +    G  F  C    CQYG  N+ Q +S +S  G L+ AG F+ATLSSA+ASLV AP+V QAL KDN++  L  F KG+G NN+PLRGY + F IA A I+I  L+V++ ++SNFF+A YALINFS FHAS  KSPGWRP++  YN WVSLF  ILC AVMF+++   AVIT+V    LY++++Y+KP+ NWGSS  A  +V++L N   L TV+ HVKN+RP  ++L+G P  R  L+D  +  TK   L IC  V     P+K+  ++M+S +   + WL  ++IKAF++   +  F +G  S +  +GLG++KPN +++GYK +W + P +  E+   +IH +FD  + V I+R+  G D S  ++                 Q +L  LE     +  AI + E EE +       KK  K N  K  PK      DG               KLV+    FK K+  G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ +  K   ++ +  SMA+LL+KFRI F D+ +I DI  K   P K+ +K                 LT A          I+D E+ +  EK+ R +RL ELL+EHS
Sbjct:   69 FLQTGETAKTDTTFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVN----RPSLLEI---HEQLAKNVTVAPGSADRVANGDGMPGDEQAENKEEDMTGVVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKES----DSM--MVDPT-NDIRIIGSITVVILLGISVAGMEWEAKAQVILLVILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKG-------EGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVAACVVRDATGSMNDTIVSGMNCNGSAACGLGYDFSRCQHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIFKGLQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNVIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAILCCAVMFVINWWAAVITYVIELFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVG--PRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLSELENYVGIIHDAFDFEIGVVIVRISQGFDISPVLQ----------------VQDELEKLEQERLALEAAIKDNECEEGKGGIRGLFKKAGKLNITKPAPK-----KDGNISSIQSMHVGEFNQKLVEASAQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIK---PNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHS 1043          

HSP 2 Score: 100.523 bits (249), Expect = 3.412e-20
Identity = 60/156 (38.46%), Postives = 90/156 (57.69%), Query Frame = 0

Query:  955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTI---------------LTQA---------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
            K+  G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ +  K   ++ +  SMA+LL+KFRI F D+ +I DI  K   P K+ +K                 LT A          I+D E+ +  EK+ R +RL ELL+EHS  +  +V++
Sbjct:  901 KQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKITRIEEEKISMASLLSKFRIKFADIHIIGDINIK---PNKESWKVFEEMIEPYRLHESHKDLTTAEKLKRESPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLS 1052          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|1709296|sp|P55015.1|S12A1_RABIT (RecName: Full=Solute carrier family 12 member 1; AltName: Full=Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; AltName: Full=Kidney-specific Na-K-Cl symporter)

HSP 1 Score: 608.216 bits (1567), Expect = 0.000e+0
Identity = 377/1013 (37.22%), Postives = 569/1013 (56.17%), Query Frame = 0

Query:   24 FYQSTSISENNMNTYVYDAD------QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFM----------EVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAK-YGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYL--AYLGNVTSKSGLFFD--NCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMA-EQMSSHI---EHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWMEDP-AGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEE--------KKKSKSN--KRVPK-----TVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL---------------------TQANISDEEVLSNLEKTNRHLRLAELLREHS 947
            F Q+   ++ + + + YD+       Q+F   TM+A+P   +YR   S+     N    RP++ E+   H    K+ +            E   G+E  +   +  K   +KFGW+ GVL                           G+++I +  +VT +T LS SA++TNG +RGGG YY+ISRSLG EFGG+IG++F  AN++AVA Y++GF ++++D+L E+    D +   VD   ND+R IG++T+  +L ++V GMEW  + + +LL++L+++  +F IGT +P+ +E K  GF  Y    F +N   +            GFF V A+FFPA TGI+AGAN+SGDL DP  AIP+GT+ A+ +T  +YI  A  +G+C + +A+G   + +      N ++  GL +D   C    CQYG  N+ Q +S +S  G L+ AG F+ATLSSA+ASLV A +V QAL KDN+Y  L  F KG+G NN+PLRGY++ F IA A I+I  L+ ++ ++SNFF+A YALINFS FHAS  KSPGWRP++  YN WVSLF  +LC AVMF+++   AVIT+V    LY++++Y+KP+ NWGSS  A  +V++L N   L TV+ HVKN+RP   +L+G P  R  L+D     TK   L IC  V     P+K+  ++M+S +   + WL  ++IKAF++   +  F +G  S +  +GLG++KPN +++GYK  W + P    E+   +IH +FD  + V I+R+  G D S       ++++          ++ L       EA  ++ E EE         KK  K N  K  PK       +          KLV+    FK K+  G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ +  K + ++ +  +MA+LL+KFRI F D+ +I DI  K +      F+ ++                     T   I+D E+ +  EK+ R +RL ELL+EHS
Sbjct:   72 FLQTGETTKTDASFHTYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSVSGPKVN----RPSLLEI---HEQLAKNVAVTPGSADRVANGEGMPGEEHAENKEEDNKAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVVIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKES----DSM--MVDPT-NDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKKSRGFFNYQASIFAENFGPSFTKG-------EGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPRGTMLAIFITTVAYIGVAICVGACVVRDATGSMNDTIISGINCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAAKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYILTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYVSALDNALELTTVEDHVKNFRPQCFVLTGGPMTRPALLDITYAFTKNSGLCICCEVFVG--PRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKKWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDIS------QVLQVQEELEKLEQERLAL-------EATIKDNECEEGNGGIRGLFKKVGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINIKPNKESWKFFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHS 1047          

HSP 2 Score: 99.7525 bits (247), Expect = 7.425e-20
Identity = 55/153 (35.95%), Postives = 89/153 (58.17%), Query Frame = 0

Query:  955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTIL---------------------TQANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVIA 1086
            K+  G IDVWWL+DDGGL+LL+P+ILT R ++ DC +R++ +  K + ++ +  +MA+LL+KFRI F D+ +I DI  K +      F+ ++                     T   I+D E+ +  EK+ R +RL ELL+EHS  +  +V++
Sbjct:  905 KQGKGTIDVWWLFDDGGLTLLIPYILTLRKKWKDCKLRIY-VGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINIKPNKESWKFFEEMIEPYRLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLS 1056          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|313104194|sp|P55017.3|S12A3_HUMAN (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl cotransporter; Short=NCC; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter)

HSP 1 Score: 593.193 bits (1528), Expect = 0.000e+0
Identity = 353/1029 (34.31%), Postives = 562/1029 (54.62%), Query Frame = 0

Query:    1 MCTDSLDNVAFQKSEKGEPPNGGFYQSTSISE-NNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHA---------------SFMEVESGKEVEDPSPKTPKGKVIKFGWIDGV---------------------------LGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSE-NVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLP-AKSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYL----AYLGNVTSKSGLFFDNCTMRE-CQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAE--QMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKI--PFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAK-----------------KASDPIKDEFKTILTQAN----ISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            +C+          S++  PP    Y S+  S   + +T+     ++F   T++ +P+  HY       + P      RPT+ +L S  + E +H                  +E E+G   E  +P+ P    ++FGW+ GV                           L  ++I +   VT +T LS+SA+STNG ++ GG Y++ISRSLG E GG+IG++F  AN++ VA + +GF +++ D+L E   P  D I        ND+R I  V++T +L +++ GMEW ++ + L  L+++VS  ++++GT +P ++ +A  GF  Y  + F +NL+     D++ P     FF + ++FFP+ TGI+AGAN+SGDL+DPA+AIPKGTL A+  T  SY+  +  IGSC + +ASG+  + +         +    G  F  CT +  C YG  N  QT+S +S    L+ AG F ATLSSA+A LV A +V Q L +D LYP +G FGKG+G N +P+RGY++ + IA A I+I  L+ ++ ++SNFF+  YALINFS FHASIT SPGWRPSF+YYNKW +LF  I+ V +MFL++   A+I    +  L L++ Y+KPE NWGSS  A  +  +L     LN V+ H+KNYRP  L+L+G P  R  LVDF    T+ LSL+IC HV      Q+M E   +++    WL+  +IKAF+S   +++   G    +   GLG++KPN++++G+K +W    PA  E    ++H +FD N  V ++R++ G + S K+   H+  +  P  D     A+VD        +A+ +EE                                 Q    F++++    ID++WL+DDGGL+LL+P++L ++ +++ C +RVF +  + + +D + +++ +LL+KFR+ FH+V ++PDI +                 + +D  KDE      + +    ISDEE+  N  K+ R +RL E++ ++S D+  +
Sbjct:   15 LCSGRFTISTLLSSDEPSPPAA--YDSSHPSHLTHSSTFCM---RTFGYNTIDVVPTYEHYAN----STQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEK-NPEEP----VRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPI--------NDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVP----DWRGPDGT--FFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVS-KMMQAHINPVFDPAEDGKEASARVD-------PKALVKEE---------------------------------QATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVF-VGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALI 973          

HSP 2 Score: 94.3597 bits (233), Expect = 3.193e-18
Identity = 50/165 (30.30%), Postives = 98/165 (59.39%), Query Frame = 0

Query:  942 LLREHSSDSETVTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAK-----------------KASDPIKDEFKTILTQAN----ISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
            L++E  + +   +++    ID++WL+DDGGL+LL+P++L ++ +++ C +RVF +  + + +D + +++ +LL+KFR+ FH+V ++PDI +                 + +D  KDE      + +    ISDEE+  N  K+ R +RL E++ ++S D+  +VI
Sbjct:  812 LVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVF-VGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVI 975          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. SwissProt
Match: gi|27151789|sp|P55018.2|S12A3_RAT (RecName: Full=Solute carrier family 12 member 3; AltName: Full=Na-Cl symporter; AltName: Full=Thiazide-sensitive sodium-chloride cotransporter)

HSP 1 Score: 592.423 bits (1526), Expect = 0.000e+0
Identity = 350/979 (35.75%), Postives = 545/979 (55.67%), Query Frame = 0

Query:   44 QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKH--ASFMEVESGKEVEDP---------SPKTPKGKVIKFGWIDGV---------------------------LGLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSEN-VPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPA-KSEAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYL----AYLGNVTSKSGLFFDNCTMRE-CQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAE--QMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKK-------------ASDPIKDEFKTILTQA--------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            ++F   T++ +P+  HY   A     P      RPT+ +L S  + E  H  A   +   G E+ D          S K+P G+ ++FGW+ GV                           L  L+I +  +VT +T LS+SA+STNG ++ GG Y++ISRSLG E GG+IG++F  AN++ VA + +GF +++ D+L EN  P  D I        ND+R IG VT+T +L +++ GMEW ++ + L  L+++VS  ++++GT +PA K +A  GF  Y+ + F +NL+     D++       FF + ++FFP+ TGI+AGAN+SGDL+DPA+AIPKGTL A+  T  SY+  +  IGSC + +ASG   + +         +    G  F  C+ +  C+YG  N  QT+S +S    L+ AG F ATLSSA+A LV A +V Q L +D LYP +G FGKG+G N +P+RGY++ + IA A I+I  L+ ++ ++SNFF+  YALINFS FHASIT SPGWRPSF+YY+KW +LF  ++ V +MFL++   A+I    +  L L++ Y+KPE NWGSS  A  +  +L     LN V+ H+KNYRP  L+L+G P  R  LVDF +  T+ LSL+IC HV      Q++ E   ++S    WL+  +IKAF+S   +++   G    +  +GLG++KPN++++G+K +W    PA  E    ++H +FD N  V I+R++ G + S  +++            +P+A V              +EE+     +S+  K+                                 ID++WL+DDGGL+LL+P++L ++ ++  C +RVF +  + + +D + +++ +LL+KFR+ FH+V ++PDI +K             A   + D FK   T A         ISDEE+  N  K+ R +RL E+L ++S D+  +
Sbjct:   52 RTFGYNTIDVVPAYEHYANSAL----PGEPRKVRPTLADLHSFLKQEGSHLHALAFDGRPGHELTDGLVEDETGANSEKSP-GEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQENGTPIVDPI--------NDIRIIGVVTVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPASKDKASKGFYSYHGDIFVQNLVP----DWRGIDG--SFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGDVNDTITPGPGLCEGLACGYGWNFTECSQQHSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIAPGKQRVPELRLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKKNWQSAHPATLEDYIGILHDAFDFNYGVCIMRMREGLNVSEALQTH----------TAPEALV--------------QEEQTSTIFQSEQGKKT--------------------------------IDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVF-VGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALI 954          

HSP 2 Score: 96.6709 bits (239), Expect = 5.307e-19
Identity = 56/183 (30.60%), Postives = 102/183 (55.74%), Query Frame = 0

Query:  924 EEVLSNLEKTNRHLRLAELLREHSSDSETVTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKK-------------ASDPIKDEFKTILTQA--------NISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
             E L+  E    H     L++E  + +   +++    ID++WL+DDGGL+LL+P++L ++ ++  C +RVF +  + + +D + +++ +LL+KFR+ FH+V ++PDI +K             A   + D FK   T A         ISDEE+  N  K+ R +RL E+L ++S D+  +++
Sbjct:  775 REGLNVSEALQTHTAPEALVQEEQTSTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVF-VGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQVEHTKRFEDMIAPFRLNDGFKDEATVAEMRRDYPWKISDEEINKNRIKSLRQVRLNEILLDYSRDAALIIL 956          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1067092449|ref|XP_018008040.1| (PREDICTED: bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Hyalella azteca])

HSP 1 Score: 807.364 bits (2084), Expect = 0.000e+0
Identity = 456/974 (46.82%), Postives = 613/974 (62.94%), Query Frame = 0

Query:   40 YDAD--QSFRK-LTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFL-PAKSEAK-YGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLA-YLGNVT------------SKSGLFF---DNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDW-MEDPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ-----------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            YD +  +S R  +T EALPSE +YR + +I         ERPT+ +L   H +  K     E     +VE    K  +GKVIKFGW  GVL                            L+++   N VTF+T +SMSAV+TNG+I GGG+YYMISRSLG EFGG+IG+MFT+ANSIAVATYIIGFV+SL DML      FD +   +D  +ND+R +G   L  ++ILA+VGM+WVTRV+ +LL LL+ SQ+D +IG F+ P   E +  GFVG+N E F+ N+L   Y DY     P     V AVFF AVTGIVAGANLSGDL+DP  AIPKGTL A+ +T+ SYI+Y  L+ +  + +ASG    +   Y  N T            S +G  +   DN T+  C++G  N  Q +  ++  G L+Y GCFAATLSSAIASLVGAPRVLQALAKD LYP + +F KGFGANNDP+ GY +VF +AF CIMIGNL+ VS+LLSNFF+A Y+LINFS FHAS  K   +RP F+YYN WVSLF  +LC+  MFL+  +TA+ITF+    LYLF+SYRKP+ NWGSS  +  +V++LR    L TV  HVKNYRP IL++SG P+ R PL+DFANL+TK LS+++C  V K+ M  K      +    WL + + KAF+++  +    EGA++   L GLGKLKPN+V++G+KSDW + D    ++    +H +F++ + +AILRL NG DYSN I          +D +SP  ++  G+   + E  D E     E KK K +     T   G  G  LP +++  +  FK K+  G IDVWWLYDDGGL+LLLP+ILT R+ +++C +R+F+LA + D LDM+ RSMANLL+KFRID+ DV++IPD     SD  + EF+ ++ +            +I++EE+ +  +KTNRH+RL ELL E+SS +  +
Sbjct:   14 YDTNYQRSLRHYITREALPSESNYRNIHTI-------HQERPTLDDL---HDSTYKPKKADEQTGNDDVEAKVQK--RGKVIKFGWKQGVLMRCLLNIWGVMLFLRISWVVGEAGLIEALVIVCFGNTVTFITAISMSAVATNGLIAGGGVYYMISRSLGPEFGGSIGLMFTIANSIAVATYIIGFVESLQDMLFFY---FDTV--MIDGGVNDIRAVGTGVLFALMILAIVGMDWVTRVQMMLLFLLLASQLDVIIGCFIGPMTDEERARGFVGWNGEVFSDNMLR-EYRDYDG--EPQNIISVFAVFFTAVTGIVAGANLSGDLKDPGSAIPKGTLLAIGITFCSYILYPFLLAANVVRDASGNTTAFKEFYYTNETVNILDSPTFTECSTTGYDYVTEDNETVLACEFGLHNSFQVMELVAAWGPLIYIGCFAATLSSAIASLVGAPRVLQALAKDRLYPGIFMFSKGFGANNDPVNGYCLVFVLAFLCIMIGNLNAVSTLLSNFFLASYSLINFSCFHASFIKIGRFRPIFQYYNLWVSLFGGVLCLVTMFLIDWITALITFLVTIGLYLFVSYRKPDVNWGSSTQSASYVSALRATLTLQTVVDHVKNYRPQILVMSGRPSSRPPLLDFANLITKNLSMMVCVEVSKD-MDWKTRTVYINKCNDWLIEKKYKAFYNLVKADRLDEGAVNLFQLAGLGKLKPNVVLLGFKSDWSVCDKQELKAYFNTLHAAFNMQLGIAILRLSNGTDYSNII----------ADDESP--KISEGI---VNEGADIETTPRAEAKKRKFSFSAAYT---GPGGAKLPKEILDGVTIFKRKQGEGTIDVWWLYDDGGLTLLLPYILTTRSNWSNCKLRIFALANRRDELDMEQRSMANLLSKFRIDYGDVIVIPDAMSGVSDASRREFQGLIEKFRTTAKCADDGVSITEEELANQKDKTNRHIRLKELLLENSSTASLI 948          

HSP 2 Score: 149.443 bits (376), Expect = 8.373e-33
Identity = 70/150 (46.67%), Postives = 105/150 (70.00%), Query Frame = 0

Query:  949 DSETVTKKRVG--FIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ-----------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
            D  T+ K++ G   IDVWWLYDDGGL+LLLP+ILT R+ +++C +R+F+LA + D LDM+ RSMANLL+KFRID+ DV++IPD     SD  + EF+ ++ +            +I++EE+ +  +KTNRH+RL ELL E+SS +  +++
Sbjct:  801 DGVTIFKRKQGEGTIDVWWLYDDGGLTLLLPYILTTRSNWSNCKLRIFALANRRDELDMEQRSMANLLSKFRIDYGDVIVIPDAMSGVSDASRREFQGLIEKFRTTAKCADDGVSITEEELANQKDKTNRHIRLKELLLENSSTASLIIM 950          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1325329382|ref|XP_023346553.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Eurytemora affinis])

HSP 1 Score: 796.193 bits (2055), Expect = 0.000e+0
Identity = 447/971 (46.04%), Postives = 590/971 (60.76%), Query Frame = 0

Query:   33 NNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKS-------EAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLG------NVTSKSGL--FFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKS---DWMEDPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLAT--KNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILT---QANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            N+  +YVYD +++   LT++A PSE  Y RL        +SS  RPTI EL +G R +            +E E+       GKV+KFGWI+GV                            GL++I++CN +T +T +SMSAV+TNG I  GG+YYMISR+LG EFGGAIG+MFT+ANSI+VATY +GFVD+LLD++ + V  F GI      RLND+R IGA  +  +L+LA++GM+WVTRV+KLLLL+LIV+Q+D V+GTFL   +        A  GF  ++ +T  KN     Y D    ++   F  V  VFF AVTGIVAGANLSGDL+DP+ AIPKGTL A+  TY SY+ +  L+   +L   SG   EY    G      N T ++       N T   C +GS+ DQ+ ++ +S TGYLVY GC+ ATLSSAIASLVGAPRVLQA+ +D +YP L  F  G GANNDP RGY++VF IAF C+MIG L+ + SL SNFF+A YAL+N SVFH+S+TKSPGWRP+FK+YNKW+SL     C+ +MFLM    A  T + ISILY  + Y KP+ANWGSS +A +F  +L N  ALN    H+K +RP ILLL G PAHR PLVDFANL+TKKLS+LICA V  E    +  + +      WL  H+I++FF    +  F +    ++ L+G+G+L PN+VMMG+ S   D  +    F+SL      SF+ N+AV ILRL NG DYS+ I  ++ +                            EE+    KK     K++  T   G+DGKPL  ++V EI  F+     GFIDVWWLYDDGGL+LLLP+IL  R +F DC +RVFSL+    ND  D Q  +  NL+ KFRID   V +IPDI KKA    K EF  +++   + +I+  ++ +N E+TNR LR AELLRE S  +E +
Sbjct:   17 NDCESYVYDQEKTLDLLTIDARPSEKSYERLEK------HSSDHRPTIEELMTGERIK------------REEEEVEKTILSGKVVKFGWIEGVFMRCLLNIWGVMLFLRLSWVIGQAGYVQGLIIITLCNAITLITAISMSAVATNGRIASGGVYYMISRALGPEFGGAIGLMFTLANSISVATYTLGFVDNLLDLVYD-VTDFQGIVADRYDRLNDLRLIGAPVIVLILLLAIIGMDWVTRVQKLLLLMLIVAQIDMVVGTFLTGGNAYVSEAERAAKGFTSWSLDTMQKNYEAKYYPDEDGKRD--SFMSVFGVFFTAVTGIVAGANLSGDLKDPSSAIPKGTLLAIGFTYISYLGFGVLVAFNFLPRTSGNAIEYANNTGIYPEITNCTEEANAVRIEYNLTETSCIFGSAYDQKVMTLMSGTGYLVYLGCYGATLSSAIASLVGAPRVLQAVGRDKIYPKLEFFAVGQGANNDPFRGYILVFLIAFGCLMIGELNTIGSLASNFFLAAYALMNLSVFHSSMTKSPGWRPTFKFYNKWISLIGAAACIVLMFLMDWRLACATIILISILYSLMLYLKPDANWGSSAEALKFSNALNNAIALNDQDDHIKTFRPQILLLCGDPAHRQPLVDFANLVTKKLSVLICAQVIPEDQKGE-KDDLKRAAHKWLSTHKIRSFFLAIPNNKFSDAVKYAVMLSGIGRLTPNMVMMGFISSNKDMNKISEYFQSLIA----SFENNIAVGILRLPNGVDYSSVIADQNTII---------------------------EEDHSVPKKNGIFQKKI-TTHFYGTDGKPLKLQIVNEIEQFRLPGGKGFIDVWWLYDDGGLTLLLPYILQTRKRFKDCKLRVFSLSNGPGNDEEDQQ--TFENLIKKFRIDVEQVFVIPDITKKAKVDTKGEFDQLISRYPEGSITKADLKANSERTNRCLRKAELLRERSRGAELI 931          

HSP 2 Score: 132.88 bits (333), Expect = 1.157e-27
Identity = 68/132 (51.52%), Postives = 89/132 (67.42%), Query Frame = 0

Query:  959 GFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLAT--KNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILT---QANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
            GFIDVWWLYDDGGL+LLLP+IL  R +F DC +RVFSL+    ND  D Q  +  NL+ KFRID   V +IPDI KKA    K EF  +++   + +I+  ++ +N E+TNR LR AELLRE S  +E +V+
Sbjct:  804 GFIDVWWLYDDGGLTLLLPYILQTRKRFKDCKLRVFSLSNGPGNDEEDQQ--TFENLIKKFRIDVEQVFVIPDITKKAKVDTKGEFDQLISRYPEGSITKADLKANSERTNRCLRKAELLRERSRGAELIVL 933          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|939697115|ref|XP_014292709.1| (PREDICTED: bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like isoform X2 [Halyomorpha halys])

HSP 1 Score: 788.104 bits (2034), Expect = 0.000e+0
Identity = 431/889 (48.48%), Postives = 584/889 (65.69%), Query Frame = 0

Query:  118 IKFGWIDGVLGL----LVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKS---EAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVT---SKSGLFFDNCTMR------ECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEG---AMSSINLTGLGKLKPNLVMMGYKSDWMEDPAG-FESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSP------DAQVDLGVLEDIAE---AIDEEEEEEEEKKKSKSNKRVPKTVLL--------------GSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ-------------ANISDEEVLSNLEKTNRHLRLAELLREHSSDS 950
            ++  W+ G  G+    ++I + NLVT  TTLSMSAVSTNG+I GGGIYYMISRSLG EFGGAIG+MFT+ANS+AV+ Y++GF +SL D+L      F   A  +D+  ND+R + A+TL  +L+LA VGM+WVTR +  LL++LI SQ+DFVIG+ +  K+   EAK GFVGYN   F KNL++ +Y  Y+  ++   FF V +VFFPAVTGIVAGANLSGDL+DP++AIPKGTL A++ T+ SYI YA ++G C L +ASG          N+T   ++ G+  +    R       C+YG  ND Q +  +++ G L+Y GCFAATLSSAIASLVGAPRVLQALAKD LYP +  F KG+GANNDP+RGY++VF IAF CI+IG L+ ++ LLSNFF+A YALINFSVFH SI+KSPGWRP+FKYYN WVSL  T+LC+AVMFL+S  TA+ TF  +  LYL++SYRKPE NWGSS  AQ +  +LR+   LN V +HVKNYRP IL+LSG P  R  L+DFANL+TK  SLLIC    K +  +++ + +    +HW + H+IKAFF +   +N  E    A + + L+GLGKL+PN+++MGYK DW        +    ++H + D  MAV ILR+  G DYS+ I           D D P      + + ++ +  +I+E   ++D+   E     +S   + +  TV +              G++G+PL    +  I  F+ K++ GFIDVWWLYDDGGL+LL+PHIL++R+Q+++C +R+FSLA + + LD   R++A LL+KFRID+ D+ +IPDI KKA      EF+ ++                 I+D E+    EKTNRH+RL ELL EHS DS
Sbjct:   14 LRLSWVVGQAGIAEGVIIICLANLVTVATTLSMSAVSTNGIIEGGGIYYMISRSLGPEFGGAIGLMFTLANSVAVSMYVVGFSESLQDLLRT----FG--AYILDNGTNDVRLVSAITLVGILVLAFVGMDWVTRTQMCLLIVLIASQLDFVIGSIVGPKTQLEEAK-GFVGYNLGVFKKNLMS-DYRLYEGVEHD--FFSVFSVFFPAVTGIVAGANLSGDLKDPSVAIPKGTLLAIITTFLSYISYALIMGGCVLRDASG----------NITFLQTEEGIPLEERWERLTECNSTCRYGLENDNQVMELVALWGPLIYGGCFAATLSSAIASLVGAPRVLQALAKDKLYPSIEFFSKGYGANNDPIRGYLLVFVIAFGCILIGQLNYIAPLLSNFFLAAYALINFSVFHVSISKSPGWRPAFKYYNAWVSLAGTLLCIAVMFLISWWTALATFFVVVTLYLYVSYRKPEVNWGSSTQAQAYYFALRSALELNKVAEHVKNYRPQILVLSGQPGARPALIDFANLITKGKSLLICTQFIKGTHSKRVHDALCDKAQHWFEQHKIKAFFVL--GENAAEETNVAKALMRLSGLGKLRPNMILMGYKRDWATCSRNELDQYFAILHVALDSYMAVGILRMAEGLDYSSMI-----------DGDDPPLSPVLERKCEIKISRNISEGQLSLDDVCSESSLSCESMKGEELSTTVPIEHTLTGKDISDLYCGAEGQPLSTHHLAAITYFRRKQQKGFIDVWWLYDDGGLTLLIPHILSRRSQYSECKLRIFSLANRRNELDRAHRNLAALLSKFRIDYSDLKIIPDITKKAKIETIKEFEGLVKNFRENSSDQGEENGLGITDAELSLLAEKTNRHMRLRELLFEHSKDS 869          

HSP 2 Score: 147.902 bits (372), Expect = 2.281e-32
Identity = 70/144 (48.61%), Postives = 98/144 (68.06%), Query Frame = 0

Query:  955 KKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILTQ-------------ANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
            K++ GFIDVWWLYDDGGL+LL+PHIL++R+Q+++C +R+FSLA + + LD   R++A LL+KFRID+ D+ +IPDI KKA      EF+ ++                 I+D E+    EKTNRH+RL ELL EHS DS  +V+
Sbjct:  731 KQQKGFIDVWWLYDDGGLTLLIPHILSRRSQYSECKLRIFSLANRRNELDRAHRNLAALLSKFRIDYSDLKIIPDITKKAKIETIKEFEGLVKNFRENSSDQGEENGLGITDAELSLLAEKTNRHMRLRELLFEHSKDSTFIVV 874          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1325329380|ref|XP_023346552.1| (bumetanide-sensitive sodium-(potassium)-chloride cotransporter-like [Eurytemora affinis])

HSP 1 Score: 787.334 bits (2032), Expect = 0.000e+0
Identity = 447/971 (46.04%), Postives = 587/971 (60.45%), Query Frame = 0

Query:   33 NNMNTYVYDADQSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIADTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKS-------EAKYGFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLG------NVTSKSGL--FFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKS---DWMEDPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSEHLVKIPFSDADSPDAQVDLGVLEDIAEAIDEEEEEEEEKKKSKSNKRVPKTVLLGSDGKPLPAKLVQEINAFKTKKRVGFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLAT--KNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILT---QANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            N+  +YVYD +++   LT++A PSE  Y RL        +SS  RPTI EL +G R + +     +                GKV+KFGWI+GV                            GL++I++CN +T +T +SMSAV+TNG I  GG+YYMISR+LG EFGGAIG+MFT+ANSI+VATY +GFVD+LLD++ + V  F GI      RLND+R IGA  +  +L+LA++GM+WVTRV+KLLL++LIV+Q+D V+GTFL   +        A  GF  ++ +T  KN     Y D     N   F  V  VFF AVTGIVAGANLSGDL+DP+ AIPKGTL A+  TY SY  +  L+   +L   SG   EYL   G      N T ++       N T   C YGS+ DQ+ ++ +S TGYLVY GC+ ATLSSAIASLVGAPRVLQA+ +D +YP L  F  G GANNDP RGY++VF IAF C+MIG L+ + SL SNFF+A YAL+N SVFH+S+TKSPGWRP+FK+YNKW+SL     C+ +MFLM    A  T + ISILY  + Y KP+ANWGSS +A +F  +L N  ALN    H+K +RP ILLL G PAHR PLVDFANL+TKKLS+LICA V  E    +  + +      WL  H+I++FF    +  F +    ++ L+G+G+L PN+VMMG+ S   D  +    F+SL      SF+ N+AV ILRL NG DYS+ I  ++ +                            EE+    KK     K++  T   G+DGKPL  ++V EI  F+     GFIDVWWLYDDGGL+LLLP+IL  R +F DC +RVFSL+    ND  D Q  +  NL+ KFRID   V +IPDI KKA    K EF  +++   + +I+  ++ +N E+TNR LR AELLRE S  +E +
Sbjct:   17 NDCESYVYDQEKTLDLLTIDARPSEKSYERLEK------HSSDHRPTIEELMTGERIKREEEEIEKT------------ILSGKVVKFGWIEGVFMRCLLNIWGVMLFLRLSWVIGQAGYVQGLIIITLCNAITLITAISMSAVATNGRIASGGVYYMISRALGPEFGGAIGLMFTLANSISVATYTLGFVDNLLDLVYD-VTDFQGIVADRYDRLNDLRLIGAPVIVLILLLAIIGMDWVTRVQKLLLVMLIVAQIDMVVGTFLTGGNAYVSEAERAAKGFTSWSLDTMQKNYEAKYYPD--EDGNRDSFMSVFGVFFTAVTGIVAGANLSGDLKDPSSAIPKGTLLAIGFTYISYAGFGVLVAFNFLPRTSGNAIEYLNNTGIYPEITNCTEEANAVRIEYNLTETSCIYGSAYDQKVMTLMSGTGYLVYLGCYGATLSSAIASLVGAPRVLQAVGRDKIYPKLEFFAVGQGANNDPFRGYILVFLIAFGCLMIGELNTIGSLASNFFLAAYALMNLSVFHSSMTKSPGWRPTFKFYNKWISLIGAAACIVLMFLMDWRLACATIILISILYSLMLYLKPDANWGSSAEALKFSNALNNAIALNDQDDHIKTFRPQILLLCGDPAHRQPLVDFANLVTKKLSVLICAQVVPEDQKGE-KDDLKRAAHKWLSTHKIRSFFLAIPNNKFSDAVKYAVMLSGIGRLTPNMVMMGFISSNKDMNKISEYFQSLIA----SFENNIAVGILRLPNGVDYSSVIADQNTII---------------------------EEDHSVPKKNGIFQKKI-TTHFYGTDGKPLKLQIVNEIEQFRLPGGKGFIDVWWLYDDGGLTLLLPYILQTRKRFKDCKLRVFSLSNGPGNDEEDQQ--TFENLIKKFRIDVEQVFVIPDITKKAKVDTKGEFDQLISRYPEGSITKADLKANSERTNRCLRKAELLRERSRGAELI 931          

HSP 2 Score: 132.88 bits (333), Expect = 1.178e-27
Identity = 68/132 (51.52%), Postives = 89/132 (67.42%), Query Frame = 0

Query:  959 GFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLAT--KNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILT---QANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
            GFIDVWWLYDDGGL+LLLP+IL  R +F DC +RVFSL+    ND  D Q  +  NL+ KFRID   V +IPDI KKA    K EF  +++   + +I+  ++ +N E+TNR LR AELLRE S  +E +V+
Sbjct:  804 GFIDVWWLYDDGGLTLLLPYILQTRKRFKDCKLRVFSLSNGPGNDEEDQQ--TFENLIKKFRIDVEQVFVIPDITKKAKVDTKGEFDQLISRYPEGSITKADLKANSERTNRCLRKAELLRERSRGAELIVL 933          

BLAST of af190129_1na+ k+ 2cl- cotransporter vs. nr
Match: gi|1108479346|emb|CRK92473.1| (CLUMA_CG006045, isoform A [Clunio marinus])

HSP 1 Score: 780.4 bits (2014), Expect = 0.000e+0
Identity = 424/1016 (41.73%), Postives = 604/1016 (59.45%), Query Frame = 0

Query:   32 ENNMNTYVYDAD--QSFRKLTMEALPSEMHYRRLASIMSDPTNSSTERPTIGELASGHRTETKHASFMEVESGKEVEDPSPKTPKGKVIKFGWIDGVL---------------------------GLLVISVCNLVTFLTTLSMSAVSTNGVIRGGGIYYMISRSLGAEFGGAIGIMFTVANSIAVATYIIGFVDSLLDMLSENVPGFDGIA-DTVDHRLNDMRWIGAVTLTCVLILAVVGMEWVTRVEKLLLLLLIVSQVDFVIGTFLPAKSEAKY--GFVGYNTETFTKNLLTTNYHDYKNPKNPPGFFQVLAVFFPAVTGIVAGANLSGDLRDPAMAIPKGTLTAVVLTYFSYIIYATLIGSCYLSEASGIEAEYLAYLGNVTSKSGLFFDNCTMRECQYGSSNDQQTLSKISVTGYLVYAGCFAATLSSAIASLVGAPRVLQALAKDNLYPYLGVFGKGFGANNDPLRGYVVVFFIAFACIMIGNLDVVSSLLSNFFVAGYALINFSVFHASITKSPGWRPSFKYYNKWVSLFATILCVAVMFLMSPVTAVITFVCISILYLFISYRKPEANWGSSRDAQQFVTSLRNLQALNTVQQHVKNYRPMILLLSGLPAHRGPLVDFANLLTKKLSLLICAHVEKESMPQKMAEQMSSHIEHWLDDHRIKAFFSITHSQNFHEGAMSSINLTGLGKLKPNLVMMGYKSDWME-DPAGFESLAKVIHHSFDINMAVAILRLQNGCDYSNKIKSE---HLVKIPFSDADSPDAQVDL----------GVLEDIAEAI--DEEEEEEEEKKKSKSNKRV-----------------------------------PKTVLLGSDGKPLPAKLVQEINAFKTKKRV-GFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILT----------QANISDEEVLSNLEKTNRHLRLAELLREHSSDSETV 953
            EN ++   YD    +SFR  T EALP   +YR + S+ +        RPT+ EL         H + +  ++     +  P+  +  +IKFGW+ GVL                           GL++IS+ ++VT +T LSMSA+STNG+I+GGG YYMISRSLG EFGG+IG++F++AN++A+A Y++GF +SLLD        F  +    +D  + D R IG++T+  +L + V+GMEW  + + +LL++L+ + VDF IG     KS++    GFVG+  E   +N    +Y   K+ +    FF V ++FFPA TGI+AGAN+SGDL+DP  AIPKGTL A+ +T  SY+  A LIG   + +ASG          NV    G  F NCT R C YG  N  Q +  +SV G L+YAGCFAATLSSA+ASLV AP+V QAL  D LYP +G F KGFG NN+P+RGYV+ F I  ACI+IG L+ ++ L+SNFF+A YALINFS FHAS+ K  GWRP F+YYN W SL   ILCVAVMFL+S + A++T  C+  LYL ++YRKP+ NWGS++ AQ +  +L ++Q LN V++HVKNYRP IL+LSG P+ R  L+ F +L+TKKLSLL+C HV K+ M QK    M      +   H++KAF+ +    +F  G+ + +  +G+GKL+PN+V+MGYKSDW   + +  E    V+H +FD+++AVAILR+  G DYS+ +  E   H+++   S   S D+  DL          G  + ++  I  D+  ++ E K+K+ S   +                                   P  +  G  G  LP +++ E+N F  K+R    IDVWWLYDDGGL+LLLP+I++ R  +  C +RVF+LA K+  L+ + RSMA+LL+KFRIDF D++++PDI KK  +   + F  ++           +  I++ E+L+  +KTNRHLRL E L EHS  S+ V
Sbjct:   88 ENLLDGNEYDEKYGRSFRHFTREALPRLDNYRNILSLQAG------TRPTLEEL---------HNASIVNKAQTLPANVDPEALQAGLIKFGWMKGVLMRCLLNIWGVMLFLRLSWVVGQAGIFQGLVIISLTSVVTTITALSMSAISTNGIIKGGGTYYMISRSLGPEFGGSIGLIFSLANAVALAMYVVGFCESLLD-------AFKTVGWKIIDAGIQDTRIIGSITVVILLFIVVIGMEWEAKAQIVLLIILLAAIVDFCIGVVWGPKSDSDISKGFVGFKLELAKENFFP-DYRFDKSIERVHDFFSVFSIFFPAATGILAGANISGDLKDPQKAIPKGTLLAIFITTLSYLGMAVLIGFVMVRDASG----------NVDEVVGNVFLNCTTRACPYGLQNSVQAMELVSVFGPLIYAGCFAATLSSALASLVSAPKVFQALGADKLYPKIGWFAKGFGGNNEPVRGYVLTFVIGIACILIGELNAIAPLISNFFLAAYALINFSTFHASLAKPVGWRPKFQYYNMWASLLGAILCVAVMFLISWIMALLTMACVLALYLVVAYRKPDVNWGSTQQAQTYKNALMSVQQLNNVEEHVKNYRPQILVLSGAPSSRPQLISFGHLITKKLSLLVCGHVVKQQMTQKYRNYMMRKSNEFFRRHKLKAFYCLVDDDDFETGSRALMQASGIGKLRPNIVLMGYKSDWQTCEYSELEKYFNVLHKAFDLHLAVAILRVPKGLDYSHVLGDEAPKHILEAQKS-LTSNDSSADLLGQDKNGSAHGSCDSLSRNISQDDSRKDSELKRKASSTSDLAMTNNVSDAPDPIDIKRKLLNSPSGKSSSKKVENDPSLLYRGPGGAELPKEIIDELNRFTQKQRKDAVIDVWWLYDDGGLTLLLPYIISTRRNWNTCRLRVFALANKHSELEFEQRSMASLLSKFRIDFSDLILLPDIIKKPEEKTVNFFNGLIKEFLATTDDDEETKITESEILAVQDKTNRHLRLREYLLEHSVKSDLV 1069          

HSP 2 Score: 137.117 bits (344), Expect = 6.861e-29
Identity = 70/158 (44.30%), Postives = 102/158 (64.56%), Query Frame = 0

Query:  940 AELLREHSSDSETVTKKRV--GFIDVWWLYDDGGLSLLLPHILTKRAQFADCHMRVFSLATKNDALDMQSRSMANLLAKFRIDFHDVVMIPDIAKKASDPIKDEFKTILT----------QANISDEEVLSNLEKTNRHLRLAELLREHSSDSETVVI 1085
            AEL +E   +    T+K+     IDVWWLYDDGGL+LLLP+I++ R  +  C +RVF+LA K+  L+ + RSMA+LL+KFRIDF D++++PDI KK  +   + F  ++           +  I++ E+L+  +KTNRHLRL E L EHS  S+ VV+
Sbjct:  914 AELPKEIIDELNRFTQKQRKDAVIDVWWLYDDGGLTLLLPYIISTRRNWNTCRLRVFALANKHSELEFEQRSMASLLSKFRIDFSDLILLPDIIKKPEEKTVNFFNGLIKEFLATTDDDEETKITESEILAVQDKTNRHLRLREYLLEHSVKSDLVVM 1071