BLAST of 3-methylcrotonyl- carboxylase vs. L. salmonis genes
Match: EMLSAG00000010863 (supercontig:LSalAtl2s:LSalAtl2s721:350793:353204:-1 gene:EMLSAG00000010863 transcript:EMLSAT00000010863 description:"maker-LSalAtl2s721-augustus-gene-2.49")

HSP 1 Score: 490.345 bits (1261), Expect = 1.818e-166
Identity = 295/664 (44.43%), Postives = 393/664 (59.19%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAAL----------DTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAF---DPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLM-----------QQVTLAWDDAEHLLKVEVSGPDRQSWHVGDD--TVEAR--RIGGR------WQVGGVPAAQVDVSGD-TITVF--DGYGLVFTRIDPL----QQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAE 622
            + + +LIANRGEIACRVM TA +MG+  VAVYSDAD  + HV MAD A +IG     QSYL  D II++ L           +GAQAIHPGYGFLSEN +F +  +  G+ F+GP  +AIR MG+K  +K +M EA VP + GYHG+DQS + L +EA KIGYP++IKAV GGGGKGMR+      F + L SA++E   +FG+  +L+EK+V  PRH+EVQVFGD     V+LFERDCS+QRRHQK+IEEAP P ++ E+R  +G+A V+AA A+ Y GAGTVEFI+D     K   F+FMEMN RLQVEHPVTE ITG DLV+WQL+ AAG+ LP  QD++ +NG SFEAR+YAED  + F+P  G L H+++   D   R ++G+R GD +S  YDPMIAK+VV GP +                 G  TN+ FL  L+ H  F  GEVDT  I R    L     +   H+  A + AL L ++  +A    +   M            Q T+  +  E    V   G DR    V D   +V+A+     GR        V G  + Q  ++ D  I +F  D   + F +  P     Q  AGA G G+ + APMPG++ +V    G +V  GD L ++ AMKME+ + A + G + +
Sbjct:   16 IINKVLIANRGEIACRVMRTANSMGIETVAVYSDADKGSMHVEMADQAYYIGPPASQQSYLVQDKIIESYLVQDKIIEVAQKSGAQAIHPGYGFLSENVEFAELCQKKGIIFVGPPASAIRDMGIKSTSKIIMTEANVPCIGGYHGEDQSFERLKSEASKIGYPIMIKAVRGGGGKGMRIALTEDEFEQQLNSAKTEGLKSFGDEVMLLEKYVQNPRHVEVQVFGDQHGNYVYLFERDCSVQRRHQKIIEEAPGPFISWEVRRKLGEAXVRAARAVXYVGAGTVEFIMD-----KDFNFYFMEMNXRLQVEHPVTEMITGTDLVKWQLQAAAGQVLPITQDDIKLNGWSFEARIYAEDPDQDFMPGAGPLHHVSYPKADDNVRIETGIRQGDEVSVHYDPMIAKLVVWGPDQ-----------------GLTTNINFLMNLSAHPQFVAGEVDTDFIPRHYSELFPKITVDHEHICEA-VTALILSESQKLADNDPFSVEMGTRLNYCFSKVYQFTVNKEPIE--ASVLFKGADRFDIKVKDSSYSVQAKLAHHPGRNCSHIICSVNGHVSKQRFIANDEEIVLFTRDIGKITFEKKVPQFITAQLNAGASGAGDAV-APMPGVIEKVNVKKGDSVKVGDTLMIMIAMKMEYVIKANKTGTIQK 653          

BLAST of 3-methylcrotonyl- carboxylase vs. L. salmonis genes
Match: EMLSAG00000011449 (supercontig:LSalAtl2s:LSalAtl2s790:139998:142185:-1 gene:EMLSAG00000011449 transcript:EMLSAT00000011449 description:"maker-LSalAtl2s790-augustus-gene-1.35")

HSP 1 Score: 422.165 bits (1084), Expect = 3.076e-139
Identity = 260/674 (38.58%), Postives = 374/674 (55.49%), Query Frame = 0

Query:    2 FDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGF-LPATGTLTHL---AFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTG-LIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEP---LMQQVTLAWD----DAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITV--------------FDGYG---LVFT----RIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLE 641
            FD ILIANRGEIACRV+ TA+ MG++ VAV+S AD  +  V MAD  ++IG     +SYL  + II A   TGAQA+HPGYGFLSEN  FV  +E  G+ FIGP+  AI+AMG K A+K +  +A V  +PG+ G+ ++++     A++I YPV+IKA +GGGGKGMR+        EA    + E K  FG+  +L+EKFV  PRH+E+Q+ GD    A++L ER+CS+QRR+QKVIEEAP+P + E  R  MG+  V  A+ IGY  AGTVEF++D         F+F+EMNTRLQVEHP+TE ITGVDLV+  +R + G  L   Q+++ I+G + E+R+YAED  K F +P+ G L      ++ P  R DSG+  G  IS FYDPMI K+  +GP+RD A+A   +AL    + G   N+  L  +   E F  G++ T  L+    D  +   +     + LA +AA+   +    A F   +    + +++   WD      E  + + +   D        DT +   I   ++        +D+  + IT               + G     L+ +    R++             +I +PMPG+++ V   VG  + +G +  V+EAMKM++SL A   G V  V A  GD VE    LV +E
Sbjct:   41 FDKILIANRGEIACRVIRTAKKMGIKTVAVHSVADGGSLFVKMADETINIGPPPVNESYLVQEKIIDAVKLTGAQAVHPGYGFLSENTSFVAKLEELGVKFIGPNSEAIKAMGDKIASKRVATDAKVNGIPGFDGEIENEEHCLHLANEIKYPVMIKASSGGGGKGMRVSWNDEQTREAYRLCKEEGKNFFGDDRLLIEKFVDSPRHVEIQLLGDKHGNAIYLNERECSIQRRNQKVIEEAPSPFLDETTRKKMGEQAVSLAKTIGYDSAGTVEFLMDSQ-----KNFYFLEMNTRLQVEHPITECITGVDLVQQMIRTSYGHPLNLKQEDIKIDGWAIESRVYAEDPFKDFGMPSIGRLYQYEEPSYLPGVRCDSGIEEGSEISMFYDPMICKLTAYGPSRDAAIATSIEALDKYVIRGVTHNIPLLRDILTEEKFCLGDLTTSYLLETYPDGFSGSKMNEKDTLRLASVAAIYYAKDNYKAQFLNNDSNPAISKKIYNTWDLIIKANEVKIPISIKFEDGAFKIQNKDTDKMIVISDNFKTSD-SFISIDIHNENITTQLISKQHNGTYRIRYKGTAFNVLILSTEAHRLNQYMIEKPKLDTSKVILSPMPGVIKSVAXNVGDLIXEGQECCVVEAMKMQNSLKAGITGTVKAVNAKEGDTVEEEQILVEIE 708          

BLAST of 3-methylcrotonyl- carboxylase vs. L. salmonis genes
Match: EMLSAG00000007092 (supercontig:LSalAtl2s:LSalAtl2s3:857289:864181:1 gene:EMLSAG00000007092 transcript:EMLSAT00000007092 description:"maker-LSalAtl2s3-snap-gene-8.38")

HSP 1 Score: 208.379 bits (529), Expect = 1.950e-57
Identity = 117/283 (41.34%), Postives = 157/283 (55.48%), Query Frame = 0

Query:  168 MRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPK---AQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDP--ACRADS-GVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLI 443
            MR+V            A SEA  AFGN  + +E++V  PRHIEVQ+ GD     VHLFERDCS+QRRHQKV+E APAP +   +R  +    V   + +GY  AGTVEF+V+ S G      FFME+N RLQVEH VTE IT VDLV+ Q++V  G  LP     Q+ +  +G++ + RL  ED  K F P TG +           R DS    +G  ISP+YD ++ KV+    T +   ++L ++L   R+ G  TN+ F+  +  H  F    VDT  I
Sbjct:    1 MRVVTKLEDVPGQFERAHSEAIKAFGNGSIFIERYVQHPRHIEVQIMGDKYGNTVHLFERDCSVQRRHQKVVEIAPAPNLDPVIRQKILDDAVHLCKHVGYSNAGTVEFLVEPSTGQH----FFMEVNARLQVEHTVTEEITCVDLVQTQIKVEEGHSLPDLNLNQESIQHHGYAIQCRLTTEDPAKNFQPDTGRIEVFRSGEGMGIRLDSASAFAGAIISPYYDSLLVKVIARASTLEATCSKLSRSLKEFRIRGVKTNIPFVLNVLQHPDFVNAAVDTWFI 279          

BLAST of 3-methylcrotonyl- carboxylase vs. L. salmonis genes
Match: EMLSAG00000008225 (supercontig:LSalAtl2s:LSalAtl2s496:326845:336125:1 gene:EMLSAG00000008225 transcript:EMLSAT00000008225 description:"maker-LSalAtl2s496-snap-gene-3.19")

HSP 1 Score: 134.42 bits (337), Expect = 8.590e-33
Identity = 106/331 (32.02%), Postives = 152/331 (45.92%), Query Frame = 0

Query:  156 LIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPK--------AQDELSI--------------NGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTIS------PFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTV-TNLAFLGALAGHEGFRKGEVDTGLIARDI--DALTTPP 454
            +IKA  GGGGKG+R   +   F +     ++E     G+P + V K     RH+EVQ+  D   TA+ LF RDCS+QRRHQK+IEEAP    +  +   M +A V+ AE +GY                      F+E+N RLQVEHP TE +  ++L   QL VA G  L +        +QD L                NGH   AR+ +E+  +GF P++GT+  L F    +++  V    ++S       F D     +   G  R+ A   L  AL    + G   T + +L  L   E F+   ++TG +   I   A TT P
Sbjct:    1 MIKASEGGGGKGIRKAASDEEFRKQYPQVQAEVP---GSP-IFVMKLAQNARHLEVQLLADEYGTAISLFGRDCSIQRRHQKIIEEAPVIVASPNVFREMEEAAVRXAELVGY----------------------FLELNPRLQVEHPCTEMVANINLPASQLMVAMGIPLHRMKSIRRLYSQDSLETESINFKCPNPRPCHNGHVIAARVTSENPDEGFKPSSGTVYELNF----KSNKNVWGYFSVSASGGLHEFADSQFGHIFSWGEDREQARENLVVALKELSIQGDFRTIVEYLIMLLEKEEFQNNSLNTGWLDTLIANKAKTTKP 301          

BLAST of 3-methylcrotonyl- carboxylase vs. SwissProt
Match: gi|108861983|sp|Q96RQ3.3|MCCA_HUMAN (RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA carboxylase biotin-containing subunit; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor)

HSP 1 Score: 553.903 bits (1426), Expect = 0.000e+0
Identity = 308/676 (45.56%), Postives = 397/676 (58.73%), Query Frame = 0

Query:    5 ILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAF---DPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTL-----WEPL------------MQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSG-----------DTITVFDGYGLVFTRI---DPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLEATEA 645
            +LIANRGEIACRVM TA+ +GV+ VAVYS+AD  + HV MAD A  IG +   QSYL  + IIQ A  + AQAIHPG GFLSEN +F +  +  G+ FIGP  +AIR MG+K  +K++M  AGVPVV GYHG+DQSD  L   A +IGYPV+IKAV GGGGKGMR+V +   F E L SAR EAK +F +  +L+EKFV  PRH+EVQVFGD    AV+LFERDCS+QRRHQK+IEEAPAPG+  E+R  +G+A V+AA+A+ Y GAGTVEFI+D         F FMEMNTRLQVEHPVTE ITG DLVEWQLR+AAGE +P +Q+E+++ GH+FEAR+YAED    F+P  G L HL+    DP+ R ++GVR GD +S  YDPMIAK+VV    R  AL +LR +L    + G  TN+ FL  L+GH  F  G V T  I +    L      A +         L L +      FTL     + P              + +TL        + V  +     S  + D T +   +G  +  G     +  V+G           +TI +F   G +   I     L   +  +  G  + APM G + +VF   G  V  GD L V+ AMKMEH++ + +DG V +V    G Q      LV  E  E+
Sbjct:   52 VLIANRGEIACRVMRTAKKLGVQTVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLSMEKIIQVAKTSAAQAIHPGCGFLSENMEFAELCKQEGIIFIGPPPSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARRIGYPVMIKAVRGGGGKGMRIVRSEQEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPAPGIKSEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSK-----HNFCFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEITLQGHAFEARIYAEDPSNNFMPVAGPLVHLSTPRADPSTRIETGVRQGDEVSVHYDPMIAKLVVWAADRQAALTKLRYSLRQYNIVGLHTNIDFLLNLSGHPEFEAGNVHTDFIPQHHKQLLLSRKAAAKESLCQAALGLILKEKAMTDTFTLQAHDQFSPFSSSSGRRLNISYTRNMTLKDGKNNVAIAVTYNHDGSYSMQIEDKTFQV--LGNLYSEGDCTYLKCSVNGVASKAKLIILENTIYLFSKEGSIEIDIPVPKYLSSVSSQETQGGPL-APMTGTIEKVFVKAGDKVKAGDSLMVMIAMKMEHTIKSPKDGTVKKVFYREGAQANRHTPLVEFEEEES 719          

BLAST of 3-methylcrotonyl- carboxylase vs. SwissProt
Match: gi|81882966|sp|Q5I0C3.1|MCCA_RAT (RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA carboxylase biotin-containing subunit; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor)

HSP 1 Score: 551.206 bits (1419), Expect = 0.000e+0
Identity = 320/679 (47.13%), Postives = 413/679 (60.82%), Query Frame = 0

Query:    5 ILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDP---ACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIAR-DIDALTTPPVIAPRHVALAGMAALGLL----------------QTGPV---AGFTLWEPLMQQVTLAWDDAEHLLKVEVS---GPDRQSWH-----VGDDTVEARRIGGRWQVGGVPA-AQVDVSGDTITVFDGYGLVFTRIDPLQQ------AAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLEATE 644
            +LIANRGEIACRV+ TA+ MGV+ VAVYS+AD  + HV MAD A  IG +   QSYL  + IIQ A  + AQAIHPGYGFLSEN +F +  +  G+ FIGP   AIR MG+K  +K++M  AGVPVV GYHG DQSD+ L   A KIGYPV+IKA+ GGGGKGMR++ +   F E L SAR EAK +F +  +L+EKFV  PRH+EVQVFGD    AV+LFERDCS+QRRHQK+IEEAPAPG+  E+R  +G+A V+AA+A+ Y GAGTVEFI+D         F+FMEMNTRLQVEHPVTE ITG DLVEWQLR+AAGE +P +Q+E+ + GH+FEAR+YAED    F+P  G L HL+  P   + R ++GVR GD +S  YDPMIAK+VV    R  AL++LR +L    + G  TN+ FL  L+GH  F  G V T  I +   D L T   IA   V     AALGL+                Q  P    +G  L     + +TL     + ++ V  +     D Q  +     +GD + E      +  V GV + ++  +  +TI +F   G +   I P+ +      A G QG G +  APM G + +VF   G  V  GD L V+ AMKMEH++ A +DG + +V  + G Q    A LV  E  E
Sbjct:   48 VLIANRGEIACRVIRTARKMGVQSVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLAMEKIIQVAKSSAAQAIHPGYGFLSENMEFAEFCKQEGIIFIGPPSTAIRDMGIKSTSKSIMAAAGVPVVEGYHGNDQSDECLKEHAGKIGYPVMIKAIRGGGGKGMRIIRSEKEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPAPGIDPEVRRRLGEAAVRAAKAVNYVGAGTVEFIMDSK-----HNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGPLVHLSTPPPDMSTRIETGVRQGDEVSVHYDPMIAKLVVWASDRQSALSKLRYSLHQYNIVGLRTNVDFLLRLSGHSEFEAGNVHTDFIPQHHKDLLPTHSTIAKESVC---QAALGLILKEKEMTSAFKLHTQDQFSPFSFSSGRRLNISYTRNMTLRSGKNDIIIAVTYNRDGSYDMQIENKLFRVLGDLSNEDGYTYLKSSVNGVASKSKFILLDNTIYLFSMEGSIEVGI-PVPKYLSPVSAEGTQG-GTI--APMTGTIEKVFVKAGDRVKAGDALMVMIAMKMEHTIKAPKDGRIKKVFFSEGAQANRHAPLVEFEEEE 714          

BLAST of 3-methylcrotonyl- carboxylase vs. SwissProt
Match: gi|20138697|sp|Q99MR8.2|MCCA_MOUSE (RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA carboxylase biotin-containing subunit; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor)

HSP 1 Score: 540.806 bits (1392), Expect = 0.000e+0
Identity = 321/680 (47.21%), Postives = 416/680 (61.18%), Query Frame = 0

Query:    5 ILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAF---DPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIAR-DIDALTTPPVIAPRHVALAGMAALGLL----------------QTGPV---AGFTLWEPLMQQVTLAWDDAEHLLKVEVSGP-------DRQSWHV-GDDTVEARRIGGRWQVGGVP-AAQVDVSGDTITVFDGYGLVFTRIDPLQQ------AAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLEATEA 645
            +LIANRGEIACRV+ TA+ MGV+ VAVYS+AD  + HV MAD A  IG +   QSYL  + IIQ A  + AQAIHPGYGFLSEN +F +  +  G+ FIGP  +AIR MG+K  +K++M  AGVPVV GYHGKDQSD  L   A KIGYPV+IKAV GGGGKGMR+V +   F E L SAR EAK +F +  +L+EKFV  PRH+EVQVFGD    AV+LFERDCS+QRRHQK+IEEAPAPG+  E+R  +G+A V+AA+A+ Y GAGTVEFI+D         F+FMEMNTRLQVEHPVTE ITG DLVEWQLR+AAGE +P +Q+E+ + GH+FEAR+YAED    F+P  G L HL+    D + R ++GVR GD +S  YDPMIAK+VV    R  AL++LR  L    + G  +N+ FL  L+GH  F  G V T  I +   D L +   IA   V     AALGL+                Q  P    +G  L     + +TL    ++ ++ V  +         D +S+ V GD + E      +  + GV   ++  +  +T+ +F   G +   I P+ +      A GAQG G +  APM G + +VF   G  V  GD L V+ AMKMEH++ A +DG + +V  + G Q    A LV  E  E+
Sbjct:   48 VLIANRGEIACRVIRTAKKMGVQSVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLAMEKIIQVAKSSAAQAIHPGYGFLSENMEFAELCKQEGIIFIGPPSSAIRDMGIKSTSKSIMAAAGVPVVEGYHGKDQSDQCLREHAGKIGYPVMIKAVRGGGGKGMRIVRSEREFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPAPGINPEVRRKLGEAAVRAAKAVKYVGAGTVEFIMDSR-----HNFYFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGPLVHLSTPSADMSTRIETGVRQGDEVSVHYDPMIAKLVVWASDRQSALSKLRYCLHQYNIVGLRSNVDFLLRLSGHPEFEAGNVHTDFIPQHHKDLLPSHSTIAKESVC---QAALGLILKEKEMTSAFKLHTQDQFSPFSFSSGRRLNISYTRNMTLRSGKSDIVIAVTYNRDGSYDMQIDNKSFRVLGDLSSEDGCTYLKSSINGVARKSKFILLDNTVHLFSMEGSIEVGI-PVPKYLSPVSAEGAQG-GTI--APMTGTIEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKAPKDGRIKKVFFSEGAQANRHAPLVEFEEEES 715          

BLAST of 3-methylcrotonyl- carboxylase vs. SwissProt
Match: gi|74853044|sp|Q54KE6.1|MCCA_DICDI (RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA carboxylase biotin-containing subunit; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor)

HSP 1 Score: 535.413 bits (1378), Expect = 0.000e+0
Identity = 305/675 (45.19%), Postives = 403/675 (59.70%), Query Frame = 0

Query:    5 ILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAF---DPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAP---------------RHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEV------SGPDRQSWHV-----------------GDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAAL 637
            ILIANRGEIACRVM TA++ GV+ VAVYS+AD  + HV+MAD +  IG +   +SYL G+ II  A  +GAQAIHPGYGFLSEN DF D  E  G+ FIGP   AI+AMG K A+K +M++AGVP +PGYHG+DQS  +L +EA KIGYPVLIKAV GGGGKGMR+VE      + + S++ EA  +FG+  VLVEK++  PRH+E+QVF D     VHLFERDCS+QRRHQK+IEEAPAP ++EE+R  MG A V AA+A+GY GAGTVEFI+        + FFFMEMNTRLQVEHP+TE IT  DLVEWQL+VA  + LP  Q++L I+GHSFEAR+YAE+    FLP TG L HL+        R ++GVR GD +S +YDPMIAK+VV    R+ AL  LR AL    + G  TN++FL  L+ H  F  GEV+TG I    ++L  P   AP               + +               + GF +   L +QV     D + ++ VE       +   + ++ V                  ++T+ A  + GR+    + +  V    DT+T+F+        I    +  GA G    + +PMPG + +V   VG  V KG  + ++EAMKMEH++ +  DG V  +     + VE    L
Sbjct:   33 ILIANRGEIACRVMRTAKSKGVKTVAVYSEADKNSLHVSMADESYLIGPAAAKESYLCGNKIIDVAKRSGAQAIHPGYGFLSENSDFADLCEREGIIFIGPPSDAIKAMGSKSASKDIMIKAGVPTIPGYHGEDQSMSVLKSEAAKIGYPVLIKAVMGGGGKGMRIVEREEDLEDGVESSKREATASFGDSRVLVEKYLVHPRHVEIQVFADRHGNCVHLFERDCSVQRRHQKIIEEAPAPHLSEELRKKMGDAAVAAAKAVGYVGAGTVEFILSAD-----NSFFFMEMNTRLQVEHPITEMITKQDLVEWQLKVAESQTLPMEQEQLKIHGHSFEARIYAENPDSDFLPGTGKLAHLSTPTPSDTLRVETGVRQGDEVSVYYDPMIAKLVVWDQDREKALRYLRNALDEYHIIGLNTNISFLKRLSTHPSFMAGEVETGFIPIHRESLMAPQ--APMSDDSLALAATSLLLKEITQQKSKEDPNSPWSSLGGFRINHNLKKQVKFNQKDNKVVVNVEFIGGGGAAANGKHNFKVTLDNGNVVEVLDAKLNQNNETISA-HVNGRF-YNNIKSVIVK---DTLTIFNEGQQYQLDIPQDVKPKGADGVLGSLVSPMPGKITKVMVNVGDMVKKGQPILLMEAMKMEHTIRSPIDGKVESLPYNVNEIVEDKKTL 695          

BLAST of 3-methylcrotonyl- carboxylase vs. SwissProt
Match: gi|110810425|sp|Q2QMG2.2|MCCA_ORYSJ (RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor)

HSP 1 Score: 529.635 bits (1363), Expect = 2.961e-179
Identity = 265/455 (58.24%), Postives = 333/455 (73.19%), Query Frame = 0

Query:    3 DTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPAC---RADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIAR-DIDALTT 452
            + +L+ANRGEIACRVM TA+ +G+  VAVYSDAD  A HV  AD AV +G     +SYL    I+ AAL TGA+AIHPGYGFLSE+ DF    +A GLTFIGP  +AIR MG K A+K +M  AGVP+VPGYHG +Q  +LL  EA+KIGYPVLIK   GGGGKGMR+V+    F +++ SA+ EA  +FG   +LVEK+++QPRHIEVQ+FGD     +HL+ERDCSLQRRHQK+IEEAPAP +T + R+ +G+A V AA+A+GY  AGTVEFIVD   G     F+FMEMNTRLQVEHPVTE I G DLVEWQ+R+A GE LP +Q+++ +NGH+FEAR+YAE+VP+GFLPATGTL H    P+    R ++GV  GDT+S  YDPMIAK+VV G +R+ AL +L+ +L+N ++ G  TN+ FL  LAGH  F KG VDT  I R   D L+T
Sbjct:   40 EKVLVANRGEIACRVMRTARRLGIPTVAVYSDADRGALHVRAADEAVRLGPPPARESYLNASAIVDAALRTGAKAIHPGYGFLSESADFAQLCKAEGLTFIGPPPSAIRDMGDKSASKRIMGAAGVPLVPGYHGAEQDIELLKLEANKIGYPVLIKPTHGGGGKGMRIVQRPEDFVDSVLSAQREAAASFGINTLLVEKYITQPRHIEVQIFGDQHGNVIHLYERDCSLQRRHQKIIEEAPAPNVTAQFRSHIGEAAVSAAKAVGYYSAGTVEFIVDTLSG----EFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRIANGECLPLSQEQVPLNGHAFEARIYAENVPRGFLPATGTLHHYRPVPSTATVRVETGVEEGDTVSMHYDPMIAKLVVWGESRNAALVKLKNSLSNFQIAGLPTNVGFLQELAGHSAFEKGLVDTHFIERYQNDLLST 490          

BLAST of 3-methylcrotonyl- carboxylase vs. SwissProt
Match: gi|20455046|sp|Q42523.2|MCCA_ARATH (RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor)

HSP 1 Score: 527.709 bits (1358), Expect = 1.309e-178
Identity = 258/446 (57.85%), Postives = 328/446 (73.54%), Query Frame = 0

Query:    2 FDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTH---LAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLI 443
             + IL+ANRGEIACR+M TA+ +G++ VAVYSDAD  + HV  AD AV IG  +   SYL G  I++AA  TGAQAIHPGYGFLSE+ DF    E +GLTFIGP  +AIR MG K A+K +M  AGVP+VPGYHG +Q  D + +EA+KIGYP++IK   GGGGKGMR+V++   FA++   A+ EA  +FG   +L+EK++++PRHIEVQ+FGD     +HL+ERDCS+QRRHQK+IEEAPAP ++E+ RA +GQA V AA A+GY  AGTVEFIVD     ++D F+FMEMNTRLQVEHPVTE I G DLVEWQ+RVA GE LP +Q E+ ++GH+FEAR+YAE+VPKGFLPATG L H   +A  P+ R ++GV  GDT+S  YDPMIAK+VV G  R  AL +L+  L+N +V G  TN+ FL  LA H+ F  G V+T  I
Sbjct:   38 IEKILVANRGEIACRIMRTAKRLGIQTVAVYSDADRDSLHVKSADEAVRIGPPSARLSYLSGVTIMEAAARTGAQAIHPGYGFLSESSDFAQLCEDSGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGHEQDIDHMKSEAEKIGYPIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILLEKYITRPRHIEVQIFGDKHGNVLHLYERDCSVQRRHQKIIEEAPAPNISEKFRANLGQAAVSAARAVGYYNAGTVEFIVD----TESDQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGEPLPLSQSEVPMSGHAFEARIYAENVPKGFLPATGVLNHYRPVAVSPSVRVETGVEQGDTVSMHYDPMIAKLVVWGGNRGEALVKLKDCLSNFQVAGVPTNINFLQKLASHKEFAVGNVETHFI 479          

BLAST of 3-methylcrotonyl- carboxylase vs. SwissProt
Match: gi|20455047|sp|Q42777.2|MCCA_SOYBN (RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor)

HSP 1 Score: 514.612 bits (1324), Expect = 1.573e-173
Identity = 307/703 (43.67%), Postives = 407/703 (57.89%), Query Frame = 0

Query:    2 FDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTL--THLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLI-----------------------ARDIDALTTPPVIAPRHVALA-----GMAALGLLQTGPVAGFTLWEPLMQQVTLAWD-------------------DAEHLLKVEVSGP---DRQSWHVGDD--TVEARRIGGRWQVGGVPAAQVD----VSGDTITVF-DGYGLVFTRIDPLQQAAGAQGDGN---LIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLE 641
             + IL+ANRGEIACR+  TA+ +G++ VAVYSDAD  + HVA AD A+ IG      SYL G  I+ AA+ +GAQAIHPGYGFLSE+ DF    E +GLTFIGP  +AIR MG K A+K +M  AGVP+VPGYHG DQ  + +  EAD+IGYPVLIK   GGGGKGMR+V     F E+  +A+ EA  +FG   +L+EK++++PRHIEVQ+FGD     +HL+ERDCS+QRRHQK+IEEAPAP ++ + RA +G A V AA+A+ Y  AGTVEFIVD      +D F+FMEMNTRLQVEHPVTE I G DLVEWQ+ VA GE LP +Q ++ ++GH+FEAR+YAE+V KGFLPATG L   H+    A R ++GV+ GD +S  YDPMIAK+VV G  R  AL +L+ +L+  +V G  TN+ FL  LA H  F  G V+T  I                       AR   +L    +I   H  LA     G + L +  + P   F +     +++ L WD                   D  +L++ E +G    + +S +V D+   VEA  +     V      Q+       G     F +  GL  +  +  Q     +   N    + APM GLV +V       V +G  + VLEAMKMEH + A   G V  +    G+QV  G+ L  ++
Sbjct:   33 IEKILVANRGEIACRITRTARRLGIQTVAVYSDADRDSLHVATADEAIRIGPPPARLSYLNGASIVDAAIRSGAQAIHPGYGFLSESADFAKLCEESGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGYDQDIEKMKLEADRIGYPVLIKPTHGGGGKGMRIVHTPDEFVESFLAAQREAAASFGVNTILLEKYITRPRHIEVQIFGDKHGNVLHLYERDCSVQRRHQKIIEEAPAPNISADFRAQLGVAAVSAAKAVNYYNAGTVEFIVDTV----SDEFYFMEMNTRLQVEHPVTEMIVGQDLVEWQILVANGEALPLSQSQVPLSGHAFEARIYAENVQKGFLPATGVLHHYHVPVSSAVRVETGVKEGDKVSMHYDPMIAKLVVWGENRAAALVKLKDSLSKFQVAGLPTNVNFLQKLANHRAFAIGNVETHFIDNYKEDLFVDANNSVSVKEAYEAARLNASLVAACLIEKEHFILARNPPGGSSLLPIWYSSP--PFRIHHQAKRRMELEWDNEYGSGSSKIMKLTITYQPDGRYLIETEQNGSPVLEVKSTYVKDNYFRVEAAGVINDVNVAVYSKDQIRHIHIWQGSCHHYFREKLGLELSEDEESQHKPKVETSANPQGTVVAPMAGLVVKVLVENKTRVEEGQPVLVLEAMKMEHVVKAPSSGYVHGLQLMVGEQVSDGSVLFSVK 729          

BLAST of 3-methylcrotonyl- carboxylase vs. SwissProt
Match: gi|61219643|sp|P0A509.1|BCCA_MYCBO (RecName: Full=Acetyl-/propionyl-coenzyme A carboxylase alpha chain; Includes: RecName: Full=Biotin carboxylase; Includes: RecName: Full=Biotin carboxyl carrier protein; Short=BCCP >gi|614130097|sp|P9WPQ2.1|BCCA_MYCTO RecName: Full=Acetyl-/propionyl-coenzyme A carboxylase alpha chain; Includes: RecName: Full=Biotin carboxylase; Includes: RecName: Full=Biotin carboxyl carrier protein; Short=BCCP >gi|614130103|sp|P9WPQ3.1|BCCA_MYCTU RecName: Full=Acetyl-/propionyl-coenzyme A carboxylase alpha chain; Includes: RecName: Full=Biotin carboxylase; Includes: RecName: Full=Biotin carboxyl carrier protein; Short=BCCP)

HSP 1 Score: 467.233 bits (1201), Expect = 5.176e-156
Identity = 303/673 (45.02%), Postives = 382/673 (56.76%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPA---CRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAG---------MAALGLLQTGPVAGF-----------TLWEPLMQQVTLAWDDAEHL-LKVEVSGPDRQSWHVGDD----TVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTR---IDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLE 641
            MFDT+L+ANRGEIA RV+ T + +G+R VAVYSD D  A+HV  AD AV +G +   +SYL    ++ AA  TGAQAIHPGYGFL+EN DF  A E A + F+GP   AI  MG K AAK  +    VPVVPG      +DD L   A ++GYPVLIK  AGGGGKGMRLV+      EAL SAR EA ++FG+  + +E+FV +PRHIEVQV  D     VHL ER+CSLQRRHQKVIEEAP+P +  + R  +G A    A  + Y GAGTVEFIV      + D FFFMEMNTRLQVEHPVTEAITG+DLVEWQLRV AGE L  AQ+++ + GH+ EAR+YAED  + FLP TG      F+PA    R DS +  G  +   YDP++ KV+ HG  R+ AL RL QALA T V G  TN+ FL  L   E  R G++DT ++       T  P  AP  V  AG           A G L   P +G+            +  PL  +    W   E   ++V     D  S  V  +    T+   R   RW       A+ D     + + D  G    R      + +A GA+     + +PMPG V  V    G  ++ GD + V+EAMKMEHSL A   G V +VL + GDQV+    L R++
Sbjct:    1 MFDTVLVANRGEIAVRVIRTLRRLGIRSVAVYSDPDVDARHVLEADAAVRLGPAPARESYLDIGKVLDAAARTGAQAIHPGYGFLAENADFAAACERARVVFLGPPARAIEVMGDKIAAKNAVAAFDVPVVPGVARAGLTDDALVTAAAEVGYPVLIKPSAGGGGKGMRLVQDPARLPEALVSARREAMSSFGDDTLFLERFVLRPRHIEVQVLADAHGNVVHLGERECSLQRRHQKVIEEAPSPLLDPQTRERIGVAACNTARCVDYVGAGTVEFIVSAQ---RPDEFFFMEMNTRLQVEHPVTEAITGLDLVEWQLRVGAGEKLGFAQNDIELRGHAIEARVYAEDPAREFLP-TGGRVLAVFEPAGPGVRVDSSLLGGTVVGSDYDPLLTKVIAHGADREEALDRLDQALARTAVLGVQTNVEFLRFLLADERVRVGDLDTAVLDERSADFTARP--APDDVLAAGGLYRQWALARRAQGDLWAAP-SGWRGGGHMAPVRTAMRTPLRSETVSVWGPPESAQVQVGDGEIDCASVQVTREQMSVTISGLRRDYRW-------AEAD---RHLWIADERGTWHLREAEEHKIHRAVGAR--PAEVVSPMPGSVIAVQVESGSQISAGDVVVVVEAMKMEHSLEAPVSGRV-QVLVSVGDQVKVEQVLARIK 653          

BLAST of 3-methylcrotonyl- carboxylase vs. SwissProt
Match: gi|74964123|sp|Q19842.1|PCCA_CAEEL (RecName: Full=Propionyl-CoA carboxylase alpha chain, mitochondrial; Short=PCCase subunit alpha; AltName: Full=Propanoyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor)

HSP 1 Score: 450.284 bits (1157), Expect = 1.625e-148
Identity = 282/686 (41.11%), Postives = 394/686 (57.43%), Query Frame = 0

Query:    2 FDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGF-LPATGTLTHLAFDPA----CRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRH--VALAGMAALGLLQ--------------TGPVAGFTLWEPLMQQVTLAWDD--AEHLLKVE-VSGP-DRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITV------------------FDGYGLVFTRIDPLQ-QAAGAQGD-GNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLE 641
            FD ILIANRGEIACRV++TA+AMG++ VAV+SD D+ + HV MAD AV +G +  A+SYLR D I+QA  DTGAQA+HPGYGFLSEN  F   +E AG  FIGP+  AI  MG K  +K +   A V ++PGY G+   +D+    +  IGYPV+IKA AGGGGKGMR+        E    ++ EA ++FG+  +LVEKF+  PRHIE+QV  D    A+ L ER+CS+QRR+QKVIEEAP+  +  EMR  MG+  V+ A+A+GY  AGTVEF+VD         F+F+EMNTRLQVEHP+TE ITG+D+V+  LRV+ G  LP  Q+++ +NG +FE+R+YAED  KGF LP+ G L+    +P      R DSG+R G  IS +YDP+I K+V HG  R+ AL R+++AL N  + G   N+  L  +   + FR G++ T  +            ++P+   V +A  +AL   +              +  VA F+     +  + +   +   EH ++VE V G  ++    +G  TV    I G   +   P   ++V G+ IT                   F    L    +  LQ     A+ D   ++ +PMPG ++ V    G  V++G +L V+EAMKM++SL A + G V  V    G  V+ G  LV LE
Sbjct:   49 FDKILIANRGEIACRVIKTARAMGIKTVAVHSDVDSNSLHVKMADEAVCVGEAPTAKSYLRADRILQAVEDTGAQAVHPGYGFLSENTKFAAELEKAGAKFIGPNSKAILDMGDKIHSKKIATAARVSMIPGYDGEIADEDMCVKVSRDIGYPVMIKASAGGGGKGMRVAWNDKQAREGYRLSKQEAASSFGDDRMLVEKFIDNPRHIEMQVLCDKHGNALWLNERECSIQRRNQKVIEEAPSSFVPPEMRRKMGEQAVQLAKAVGYDSAGTVEFLVDSQ-----RNFYFLEMNTRLQVEHPITECITGIDIVQQMLRVSYGHPLPITQEQVPLNGWAFESRVYAEDPYKGFGLPSVGRLSRYV-EPKHVDGVRCDSGIREGSEISIYYDPLICKLVTHGDNREQALNRMQEALDNYVIRGVTHNIPLLRDIVQEKRFRTGDITTKYLPEVYPEGFQGTSLSPKEQDVVIAFASALNARKLARANQFLNQNKQRSTHVASFSKTYKFVSSLPVKEGERPTEHAVEVEFVEGSANKAQVRIGGKTVT---ISGDLNLSH-PVNSIEVDGEHITTQIVGKRAGEITVLYKGTPFKVKVLPEQAVKYLQYMKEKAKVDLSTVVLSPMPGAIKNVNVKPGDMVSEGQELVVMEAMKMQNSLHAGKTGRVKAVNVKVGATVDEGEVLVELE 724          

BLAST of 3-methylcrotonyl- carboxylase vs. SwissProt
Match: gi|74846243|sp|Q612F5.1|PCCA_CAEBR (RecName: Full=Propionyl-CoA carboxylase alpha chain, mitochondrial; Short=PCCase subunit alpha; AltName: Full=Propanoyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor)

HSP 1 Score: 447.203 bits (1149), Expect = 4.217e-147
Identity = 280/688 (40.70%), Postives = 387/688 (56.25%), Query Frame = 0

Query:    2 FDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGK-TAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGF-LPATGTLTHLAFDP----ACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLI---------------ARDIDALTTPPVIAPRHVALAG-MAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGR-WQVGG-----VPAAQVDVSGDTITV------------------FDGYGLVFTRIDPLQ-QAAGAQGD-GNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLE 641
            FD ILIANRGEIACRV++TA+AMG++ VAV+SD D+ + HV MAD A+ +G +  A+SYLR D I+QA  DTGAQA+HPGYGFLSEN  F   +E AG  FIGP+  AI  MG K  +K +   A V ++PGY G+   +D     + +IGYPV+IKA AGGGGKGMR+        E    ++ EA ++FG+  +LVEKF+  PRHIE+Q+  D    A+ L ER+CS+QRR+QKVIEEAP+  +  EMR  MG+  V+ A+A+GY  AGTVEF+VD         F+F+EMNTRLQVEHP+TE ITG+D+V+  LRVA G  LP  Q+++ +NG +FE+R+YAED  KGF LP+ G L+    +P      R DSG+R G  IS +YDP+I K+V HG  R  AL R+++AL N  + G   N+  L  +   + FR G++ T  +               A +   +     +  R +A A         ++  VA F+     +  +            VEVS  D      GD       IGG+   + G     +P   ++V+G+ IT                   F    L    +  LQ     A+ D   ++ +PMPG ++ V    G  V++G +L V+EAMKM++SL A + G V  V    G  V+ G  LV LE
Sbjct:   63 FDKILIANRGEIACRVIKTAKAMGIKTVAVHSDVDSNSLHVKMADEAICVGEAPTAKSYLRVDRILQAVEDTGAQAVHPGYGFLSENTKFAAELEKAGAKFIGPNSKAILDMGDKIHSKKIATAARVSMIPGYDGEIPEEDFCVKVSREIGYPVMIKASAGGGGKGMRVAWNDKQAREGYRLSKQEAASSFGDDRMLVEKFIDNPRHIEMQILCDKHGNALWLNERECSIQRRNQKVIEEAPSSFVPPEMRRKMGEQAVQLAKAVGYDSAGTVEFLVDSQ-----RNFYFLEMNTRLQVEHPITECITGIDIVQQMLRVAYGHSLPLTQEQVPLNGWAFESRVYAEDPYKGFGLPSVGRLSKYV-EPRHVDGVRCDSGIREGSEISIYYDPLICKLVTHGDNRQQALDRMQEALDNYVIRGVTHNIPLLRDIVQEKRFRSGDITTKYLPEVYPEGFQGTVLTHAEEKTVIAFAAALNARKLARANQFLNQNRQRSTHVASFSKTYKFVSSLPAKEGQRPTEHAVEVSFVD------GDANKAKVSIGGKVIDISGNLSLSLPVNSIEVNGEHITTQIVGKRAGEITVLYKGTPFKVQVLPEQAVKYLQYMKEKAKVDLSTVVLSPMPGAIKNVNVKPGDMVSEGQELVVMEAMKMQNSLHAGKTGRVKAVNVKVGATVDEGEVLVELE 738          

BLAST of 3-methylcrotonyl- carboxylase vs. nr
Match: gi|640634663|ref|WP_025062654.1| (acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha [Sulfitobacter guttiformis] >gi|751952096|gb|KIN73009.1| Methylcrotonyl-CoA carboxylase, alpha subunit [Sulfitobacter guttiformis KCTC 32187])

HSP 1 Score: 1120.92 bits (2898), Expect = 0.000e+0
Identity = 566/641 (88.30%), Postives = 591/641 (92.20%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLE 641
            MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMAD AVHIGGS PA SYLRGDVIIQAALDTGAQAIHPGYGFLSENP FVDAVEAAGL FIGPSG+AIRAMGLKDAAK LM+EAGVPVVPGYHG DQ DDLLA EADKIGYPV+IKAVAGGGGKGMRLVE AG F  ALASARSEAK AFGN DVLVEKFVSQPRHIEVQVFGDGK AVHLFERDCSLQRRHQKVIEEAPAP M +++R AMGQA V+AAEAIGYKGAGTVEFIVDGS GLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLP  Q+ELSI GH+FEARLYAEDVP GFLPATGTLTHL FDPACRADSGVRSGDTISPFYDPMIAKV+VHGPTRDVALARLRQALA T V GTVTNLAFLGALAGH+GFR+GEVDTGLIARDIDALT  P +A RHVALAGMAA+GLL+TGPVAGFTLWEPL+QQV L W D   LLKVEV  PD+Q WH+GD+TVEA R GGRWQ+GG PAA+V V+GD ITVFDGYGL+FTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFA+VGQ VT GD+LAVLEAMKMEHSLLAARDGVVAEVLA AGDQVEAGAALVRLE
Sbjct:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADTAVHIGGSAPADSYLRGDVIIQAALDTGAQAIHPGYGFLSENPSFVDAVEAAGLVFIGPSGSAIRAMGLKDAAKGLMIEAGVPVVPGYHGTDQRDDLLAGEADKIGYPVMIKAVAGGGGKGMRLVEDAGDFLAALASARSEAKAAFGNSDVLVEKFVSQPRHIEVQVFGDGKQAVHLFERDCSLQRRHQKVIEEAPAPDMPQDVRDAMGQAAVRAAEAIGYKGAGTVEFIVDGSGGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPMRQEELSITGHAFEARLYAEDVPAGFLPATGTLTHLVFDPACRADSGVRSGDTISPFYDPMIAKVIVHGPTRDVALARLRQALARTEVAGTVTNLAFLGALAGHDGFRRGEVDTGLIARDIDALTAVPQVATRHVALAGMAAMGLLETGPVAGFTLWEPLVQQVMLGWGDETGLLKVEVIDPDQQRWHIGDETVEALRRGGRWQIGGQPAARVSVAGDRITVFDGYGLIFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFASVGQVVTMGDRLAVLEAMKMEHSLLAARDGVVAEVLAQAGDQVEAGAALVRLE 641          

BLAST of 3-methylcrotonyl- carboxylase vs. nr
Match: gi|640632267|ref|WP_025060267.1| (acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha [Sulfitobacter donghicola] >gi|659498350|gb|KEJ89412.1| 3-methylcrotonyl-CoA carboxylase subunit alpha [Sulfitobacter donghicola DSW-25 = KCTC 12864 = JCM 14565] >gi|751948281|gb|KIN69229.1| Methylcrotonyl-CoA carboxylase, alpha subunit [Sulfitobacter donghicola DSW-25 = KCTC 12864 = JCM 14565])

HSP 1 Score: 1115.91 bits (2885), Expect = 0.000e+0
Identity = 558/642 (86.92%), Postives = 593/642 (92.37%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLEA 642
            MFDTILIANRGEIACRV+ETAQAMGVRCVAVYSDADAAAKHVA+ADVAVHIGGS PA+SYLRGDVIIQ ALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPS  AIRAMGLKDAAKALM+EAGVPVVPGYHG DQS+ LLA EA KIGYPVLIKAVAGGGGKGMRLVE AG FAEALASARSEA+TAFGN DVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMG+AGV+AA+AIGYKGAGTVEFIVDGS GLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGE +PKAQDEL+INGHSFEARLYAEDVPKGFLPATGTLTHLAFDP CRADSGVRSGDTISPFYDPMIAKV+VHGPTRDVALARLR+ALA   V GTVTNLAFLGALA H GFR GEVDTGLIARDIDALT  P +APRHVALAGMAAL +LQ G   GF+LW+PL++QVTL W    HL+KVEV GPD+Q WH+ D+ ++ARR+GG+WQ+GG PA +V ++GD ITVFDGYGL+F R+D L Q  GAQGDGNLIEAPMPGLVREVFA VGQ V+KGDKLA+LEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLEA
Sbjct:    1 MFDTILIANRGEIACRVIETAQAMGVRCVAVYSDADAAAKHVALADVAVHIGGSAPAESYLRGDVIIQTALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSADAIRAMGLKDAAKALMIEAGVPVVPGYHGSDQSNVLLAEEAGKIGYPVLIKAVAGGGGKGMRLVEEAGSFAEALASARSEAQTAFGNADVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGEAGVRAAKAIGYKGAGTVEFIVDGSGGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEAMPKAQDELTINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPDCRADSGVRSGDTISPFYDPMIAKVIVHGPTRDVALARLRKALAGAEVAGTVTNLAFLGALAAHGGFRAGEVDTGLIARDIDALTNQPAVAPRHVALAGMAALNILQGGAEVGFSLWDPLLRQVTLEWAGEAHLIKVEVQGPDQQLWHIQDEEIQARRVGGQWQIGGQPAPKVSIAGDRITVFDGYGLIFDRLDLLMQGGGAQGDGNLIEAPMPGLVREVFAAVGQQVSKGDKLAILEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLEA 642          

BLAST of 3-methylcrotonyl- carboxylase vs. nr
Match: gi|740054860|ref|WP_037904492.1| (acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha [Sulfitobacter mediterraneus])

HSP 1 Score: 1037.33 bits (2681), Expect = 0.000e+0
Identity = 530/640 (82.81%), Postives = 561/640 (87.66%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRL 640
            MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDAD AAKHV MAD+AVHIGGS PA+SYL+GDVIIQAALDTGAQAIHPGYGFLSENPDFV+AVE AGLTFIGPS +AIRAMGLKDAAKALMVEAGVPVVPGYHG DQ D LLAAEA KIGYPVLIKAVAGGGGKGMRLVEA GGF  AL SARSEAKTAFGN DVLVEKFV+ PRHIEVQVFGDG  AVHLFERDCSLQRRHQKVIEEAPAP M +++R AMGQA V+AAEAIGY GAGTVEFIVDGS GL+ADGFFFMEMNTRLQVEHPVTE ITGVDLVEWQLRVAAGE LPK Q +LSI+GH+FEARLYAEDVPKGFLPATGTLTHL F P  RADSGVR+GDTISPFYDPMIAKV+VHGP RDVAL+RLR AL+  +VGGTVTNLAFLGALAGHEGF KGEVDTGLIARDID LT  P IAPRH  LAGMAALGLL T    GFTLW  L +Q+ L W   EHLLKV V  PD Q W VG+  V+A R GGRW +GG+PAA V  +G T+TVF+GYG+ F  IDPL +AAGAQGDGNLIEAPMPGLVR V A VGQAV KGD+LAVLEAMKMEHSLLAARDGVVAEVLA AGDQVEAGAALVRL
Sbjct:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADRAAKHVQMADLAVHIGGSAPAESYLKGDVIIQAALDTGAQAIHPGYGFLSENPDFVEAVEKAGLTFIGPSASAIRAMGLKDAAKALMVEAGVPVVPGYHGADQDDALLAAEAGKIGYPVLIKAVAGGGGKGMRLVEAPGGFQAALESARSEAKTAFGNADVLVEKFVTMPRHIEVQVFGDGTKAVHLFERDCSLQRRHQKVIEEAPAPDMPQDVRDAMGQAAVRAAEAIGYAGAGTVEFIVDGSGGLRADGFFFMEMNTRLQVEHPVTELITGVDLVEWQLRVAAGESLPKQQGDLSISGHAFEARLYAEDVPKGFLPATGTLTHLQFTPQTRADSGVRAGDTISPFYDPMIAKVIVHGPNRDVALSRLRAALSGCQVGGTVTNLAFLGALAGHEGFGKGEVDTGLIARDIDMLTAAPEIAPRHAVLAGMAALGLLDTQAETGFTLWRALRRQIMLGWGGEEHLLKVRVISPDEQHWTVGEGEVQALRRGGRWTIGGLPAADVATAGATVTVFEGYGMAFDVIDPLSRAAGAQGDGNLIEAPMPGLVRAVDAKVGQAVAKGDRLAVLEAMKMEHSLLAARDGVVAEVLATAGDQVEAGAALVRL 640          

BLAST of 3-methylcrotonyl- carboxylase vs. nr
Match: gi|497510672|ref|WP_009824870.1| (acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha [Sulfitobacter sp. NAS-14.1] >gi|83841864|gb|EAP81033.1| methylcrotonyl-CoA carboxylase, alpha subunit [Sulfitobacter sp. NAS-14.1])

HSP 1 Score: 1026.93 bits (2654), Expect = 0.000e+0
Identity = 530/641 (82.68%), Postives = 555/641 (86.58%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLE 641
            MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDAD AAKHVAMAD AVHIGGS PA SYLRG+VIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGL FIGPS  AIRAMGLKDAAKALMVEAGVPVVPGYHG DQ D LLAAEADKIGYPVLIKAVAGGGGKGMRLVEAA GF  AL SARSEAKTAFGN DVLVEKFV++PRHIEVQVFGDGK AVHLFERDCSLQRRHQKVIEEAPAPGMT EMR AMGQA V AAEAIGY GAGT+EFIVDGS GL+ DGFFFMEMNTRLQVEHPVTEAITGVDLV WQLRVAAGE LP  QDELSI+GH+FEARLYAEDVPKGFLPATGTLTHL F PACRADSGVR+GDTISPFYDPMIAKV+VHG TR++AL+RLR AL    V GTVTNLAFLGALA HEGF  G+VDTGLIARDIDALT  P +APRH ALAGMAAL LL     AGFTLW+PL + V L W   +HLL+V+V   D Q W V    V A+RIGGRW VGG  AA V V+   +TVFDGYGLVF  IDPL +AAG  GDGNLIEAPMPGLVR V A VGQ VTKGD+LAVLEAMKMEHSLLAARDGV+AEVLA AGDQVEAG ALVRLE
Sbjct:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADRAAKHVAMADTAVHIGGSAPADSYLRGEVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLVFIGPSAKAIRAMGLKDAAKALMVEAGVPVVPGYHGADQDDALLAAEADKIGYPVLIKAVAGGGGKGMRLVEAADGFQAALESARSEAKTAFGNADVLVEKFVTKPRHIEVQVFGDGKKAVHLFERDCSLQRRHQKVIEEAPAPGMTPEMREAMGQAAVTAAEAIGYAGAGTIEFIVDGSGGLQTDGFFFMEMNTRLQVEHPVTEAITGVDLVAWQLRVAAGEGLPLRQDELSIHGHAFEARLYAEDVPKGFLPATGTLTHLQFTPACRADSGVRAGDTISPFYDPMIAKVIVHGDTREIALSRLRAALTGCEVAGTVTNLAFLGALAAHEGFAAGDVDTGLIARDIDALTQTPEVAPRHAALAGMAALSLLDPAGGAGFTLWQPLRRAVGLTWGGEDHLLQVQVVSADHQIWTVEGTEVVAQRIGGRWSVGGQQAAAVSVAEGRVTVFDGYGLVFDVIDPLARAAGGHGDGNLIEAPMPGLVRVVDAKVGQTVTKGDRLAVLEAMKMEHSLLAARDGVIAEVLAQAGDQVEAGVALVRLE 641          

BLAST of 3-methylcrotonyl- carboxylase vs. nr
Match: gi|1056101974|ref|WP_067625715.1| (MULTISPECIES: acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha [Sulfitobacter] >gi|1024466538|gb|KZX98021.1| 3-methylcrotonyl-CoA carboxylase [Sulfitobacter sp. HI0021] >gi|1024472113|gb|KZY02915.1| 3-methylcrotonyl-CoA carboxylase [Sulfitobacter sp. HI0027] >gi|1024619162|gb|KZZ03039.1| 3-methylcrotonyl-CoA carboxylase [Sulfitobacter sp. HI0076])

HSP 1 Score: 1026.16 bits (2652), Expect = 0.000e+0
Identity = 526/644 (81.68%), Postives = 557/644 (86.49%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLEATE 644
            MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADA AKHVAMAD AVHIGGS PA+SYL+GDVIIQAALDTGAQAIHPGYGFLSENPDFV+AV AAGLTFIGPS  AIRAMGLKDAAKALMVEAGVPVVPGYHG DQ D LLA EA+KIGYPVLIKAVAGGGGKGMRLVE   GF  AL SARSEAKTAFGNPDVLVEKFV++PRHIEVQVFGDG  AVHLFERDCSLQRRHQKVIEEAPAPGMTE+MRAAMGQA V+AAEAIGY GAGTVEFIVDGS GL  DGFFFMEMNTRLQVEHPVTE +TG+DLVEWQLRVAAGE LPKAQDEL+INGH+FEARLYAEDVPKGFLPATGTLTHL+F P  RADSGVR+GDTISPFYDPMIAKV+VHG TRDVAL+RLR AL    VGGTVTNLAFLGALAGHEGF KG+VDTGLIARDIDALT  P +  RH ALAGMAALGL Q    AGF LW PL +QV L  D  EHLLKV V   D Q W V D  + A RIGGRW++ G  A  V V+GD +TVFD YG+ F  IDPL +AA A GDGN++EAPMPGLVR + A VGQ+V KGD+LAVLEAMKMEHSLLAARDGVVAEVLA  GDQVEAGAALVRLEA E
Sbjct:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAGAKHVAMADAAVHIGGSAPAESYLKGDVIIQAALDTGAQAIHPGYGFLSENPDFVEAVAAAGLTFIGPSAKAIRAMGLKDAAKALMVEAGVPVVPGYHGADQDDALLAEEAEKIGYPVLIKAVAGGGGKGMRLVEEPKGFQAALDSARSEAKTAFGNPDVLVEKFVTKPRHIEVQVFGDGTHAVHLFERDCSLQRRHQKVIEEAPAPGMTEDMRAAMGQAAVRAAEAIGYAGAGTVEFIVDGSGGLSTDGFFFMEMNTRLQVEHPVTELVTGIDLVEWQLRVAAGEALPKAQDELTINGHAFEARLYAEDVPKGFLPATGTLTHLSFTPQTRADSGVRAGDTISPFYDPMIAKVIVHGATRDVALSRLRAALKGCEVGGTVTNLAFLGALAGHEGFAKGDVDTGLIARDIDALTAQPEVPGRHFALAGMAALGLDQPQAEAGFNLWRPLRRQVMLGHDGEEHLLKVRVLSEDMQLWTVADHELRAERIGGRWKIDGHVAPPVSVAGDMVTVFDAYGIAFGVIDPLARAAAAHGDGNIVEAPMPGLVRAIDAEVGQSVAKGDRLAVLEAMKMEHSLLAARDGVVAEVLAQPGDQVEAGAALVRLEAEE 644          

BLAST of 3-methylcrotonyl- carboxylase vs. nr
Match: gi|1024659889|gb|KZZ29896.1| (3-methylcrotonyl-CoA carboxylase [Sulfitobacter sp. HI0082])

HSP 1 Score: 1025.77 bits (2651), Expect = 0.000e+0
Identity = 525/644 (81.52%), Postives = 557/644 (86.49%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLEATE 644
            MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADA AKHVAMAD AVHIGGS PA+SYL+GDVI+QAALDTGAQAIHPGYGFLSENPDFV+AV AAGLTFIGPS  AIRAMGLKDAAKALMVEAGVPVVPGYHG DQ D LLA EA+KIGYPVLIKAVAGGGGKGMRLVE   GF  AL SARSEAKTAFGNPDVLVEKFV++PRHIEVQVFGDG  AVHLFERDCSLQRRHQKVIEEAPAPGMTE+MRAAMGQA V+AAEAIGY GAGTVEFIVDGS GL  DGFFFMEMNTRLQVEHPVTE +TGVDLVEWQLRVAAGE LPKAQDEL+INGH+FEARLYAEDVPKGFLPATGTLTHL+F P  RADSGVR+GDTISPFYDPMIAKV+VHG TRDVAL+RLR AL    VGGTVTNLAFLGALAGHEGF KG+VDTGLIARDI+ALT  P +  RH ALAGMAALGL Q    AGF LW PL +QV L  D  EHLLKV V   D Q W V D  + A RIGGRW++ G  A  V V+GD +TVFD YG+ F  IDPL +AA A GDGN++EAPMPGLVR + A VGQ+V KGD+LAVLEAMKMEHSLLAARDGVVAEVLA  GDQVEAGAALVRLEA E
Sbjct:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAGAKHVAMADAAVHIGGSAPAESYLKGDVIVQAALDTGAQAIHPGYGFLSENPDFVEAVAAAGLTFIGPSAKAIRAMGLKDAAKALMVEAGVPVVPGYHGADQDDALLAEEAEKIGYPVLIKAVAGGGGKGMRLVEEPKGFQAALDSARSEAKTAFGNPDVLVEKFVTKPRHIEVQVFGDGTRAVHLFERDCSLQRRHQKVIEEAPAPGMTEDMRAAMGQAAVRAAEAIGYAGAGTVEFIVDGSGGLSTDGFFFMEMNTRLQVEHPVTELVTGVDLVEWQLRVAAGEALPKAQDELTINGHAFEARLYAEDVPKGFLPATGTLTHLSFTPQTRADSGVRAGDTISPFYDPMIAKVIVHGATRDVALSRLRAALKGCEVGGTVTNLAFLGALAGHEGFAKGDVDTGLIARDINALTAQPEVPGRHFALAGMAALGLDQPQAEAGFNLWRPLRRQVMLGHDGEEHLLKVRVLSEDMQLWTVADHELRAERIGGRWKIDGHVAPAVSVAGDVVTVFDAYGIAFDVIDPLARAAAAHGDGNIVEAPMPGLVRAIDAEVGQSVAKGDRLAVLEAMKMEHSLLAARDGVVAEVLAQPGDQVEAGAALVRLEAEE 644          

BLAST of 3-methylcrotonyl- carboxylase vs. nr
Match: gi|1225740147|ref|WP_093739425.1| (acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha [Sulfitobacter delicatus] >gi|1086545858|emb|SDF41546.1| 3-methylcrotonyl-CoA carboxylase alpha subunit [Sulfitobacter delicatus])

HSP 1 Score: 1025 bits (2649), Expect = 0.000e+0
Identity = 524/642 (81.62%), Postives = 555/642 (86.45%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLEA 642
            MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADA AKHVAMAD AVHIGGS PA+SYL+GDVIIQAALD+GAQAIHPGYGFLSENPDFV+AVEAAGL F+GPS  AIRAMGLKDAAKALMVEAGVPVVPGYHG DQ D LLA EA+KIGYPVLIKAVAGGGGKGMRLVE AG F EAL SARSEAKTAFGN DVLVEKFV++PRHIEVQVFGDG  AVHLFERDCSLQRRHQKVIEEAPAPGMTE MR+AMG+A V+AAEAIGY GAGTVEFIVD S GL ADGFFFMEMNTRLQVEHPVTE +TGVDLVEWQLRVAAGE LPK QDEL+I GH+FEARLYAEDVPKGFLPATGTLTHL+F P  RADSGVR+GDTISPFYDPMIAKV+VHGPTRDVAL+RLR AL    VGGTVTNLAFLGALAGH GF KG+VDTGLIARDIDALT  P +  RH ALAGMAALGLL     AGF LW PL +QV L  D  E+LLKV V   D Q W VGD  V A RIGGRW+V G  A  V V+GD +TVFD YG+ F  IDPL +AA A GDGN++EAPMPGLVR + A VGQ VTKGD+LAVLEAMKMEHSLLAARDGVVAEVLA  GDQVEAGAALVRLEA
Sbjct:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAGAKHVAMADAAVHIGGSAPAESYLKGDVIIQAALDSGAQAIHPGYGFLSENPDFVEAVEAAGLVFVGPSAKAIRAMGLKDAAKALMVEAGVPVVPGYHGADQDDALLAREAEKIGYPVLIKAVAGGGGKGMRLVEKAGSFQEALDSARSEAKTAFGNSDVLVEKFVTKPRHIEVQVFGDGTHAVHLFERDCSLQRRHQKVIEEAPAPGMTEAMRSAMGEAAVRAAEAIGYAGAGTVEFIVDSSGGLSADGFFFMEMNTRLQVEHPVTELVTGVDLVEWQLRVAAGEALPKQQDELTIEGHAFEARLYAEDVPKGFLPATGTLTHLSFTPQTRADSGVRAGDTISPFYDPMIAKVIVHGPTRDVALSRLRAALKGCEVGGTVTNLAFLGALAGHAGFAKGDVDTGLIARDIDALTAQPAVPGRHFALAGMAALGLLDPAAEAGFNLWRPLRRQVMLGRDGEEYLLKVRVLSEDVQLWTVGDSEVRAERIGGRWKVDGHVAPTVSVAGDVVTVFDAYGIAFDVIDPLAKAAAAHGDGNIVEAPMPGLVRAIDAEVGQRVTKGDRLAVLEAMKMEHSLLAARDGVVAEVLAQPGDQVEAGAALVRLEA 642          

BLAST of 3-methylcrotonyl- carboxylase vs. nr
Match: gi|740095212|ref|WP_037944469.1| (acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha [Sulfitobacter sp. CB2047] >gi|668745343|gb|KFC26556.1| 3-methylcrotonyl-CoA carboxylase [Sulfitobacter sp. CB2047])

HSP 1 Score: 1025 bits (2649), Expect = 0.000e+0
Identity = 529/641 (82.53%), Postives = 554/641 (86.43%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLE 641
            MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDAD AAKHVAMAD AVHIGGS PA SYLRG+VIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGL FIGPS  AIRAMGLKDAAKALMVEAGVPVVPGYHG DQ D LLAAEADKIGYPVLIKAVAGGGGKGMRLVEAA GF  AL SARSEAKTAFGN DVLVEKFV++PRHIEVQVFGDGK AVHLFERDCSLQRRHQKVIEEAPAPGMT EMR AMGQA V AAEAIGY GAGT+EFIVDGS GL+ DGFFFMEMNTRLQVEHPVTEAITGVDLV WQLRVAAGE LP  QDELSI+GH+FEARLYAEDVPKGFLPATGTLTHL F PACRADSGVR+GDTISPFYDPMIAKV+VHG TR++AL+RLR AL    V GTVTNLAFLGALA HEGF  G+VDTGLIARDIDALT  P + PRH ALAGMAAL LL     AGFTLW+PL + V L W   +HLL+V+V   D Q W V    V A+RIGGRW +GG  AA V V+   +TVFDGYGLVF  IDPL +AAG  GDGNLIEAPMPGLVR V A VGQ VTKGD+LAVLEAMKMEHSLLAARDGVVAEVLA AGDQVEAG ALVRLE
Sbjct:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADRAAKHVAMADTAVHIGGSAPADSYLRGEVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLVFIGPSAKAIRAMGLKDAAKALMVEAGVPVVPGYHGADQDDALLAAEADKIGYPVLIKAVAGGGGKGMRLVEAADGFQAALESARSEAKTAFGNADVLVEKFVTKPRHIEVQVFGDGKKAVHLFERDCSLQRRHQKVIEEAPAPGMTPEMREAMGQAAVTAAEAIGYAGAGTIEFIVDGSGGLQTDGFFFMEMNTRLQVEHPVTEAITGVDLVAWQLRVAAGEGLPLRQDELSIHGHAFEARLYAEDVPKGFLPATGTLTHLQFTPACRADSGVRAGDTISPFYDPMIAKVIVHGDTREIALSRLRAALTGCEVAGTVTNLAFLGALAAHEGFAAGDVDTGLIARDIDALTQAPEVEPRHAALAGMAALSLLDPAAGAGFTLWQPLRRAVGLTWGGEDHLLQVQVVSADHQIWTVDGTEVVAQRIGGRWSLGGQQAAAVSVAEGRVTVFDGYGLVFDVIDPLARAAGGHGDGNLIEAPMPGLVRVVDAKVGQTVTKGDRLAVLEAMKMEHSLLAARDGVVAEVLAQAGDQVEAGVALVRLE 641          

BLAST of 3-methylcrotonyl- carboxylase vs. nr
Match: gi|1032584008|ref|WP_064215553.1| (acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha [Sulfitobacter pontiacus] >gi|1032111689|gb|OAN84080.1| 3-methylcrotonyl-CoA carboxylase [Sulfitobacter pontiacus])

HSP 1 Score: 1024.62 bits (2648), Expect = 0.000e+0
Identity = 529/641 (82.53%), Postives = 553/641 (86.27%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLE 641
            MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDAD AAKHVAMAD AVHIGGS PA SYLRG+VIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGL FIGPS  AIRAMGLKDAAKALMVEAGVPVVPGYHG DQ D LLAAEADKIGYPVLIKAVAGGGGKGMRLVEAA GF  AL SARSEAKTAFGN DVLVEKFV++PRHIEVQVFGDGK AVHLFERDCSLQRRHQKVIEEAPAPGMT EMR AMGQA V AAEAIGY GAGT+EFIVDGS GL+ DGFFFMEMNTRLQVEHPVTEAITGVDLV WQLRVAAGE LP  QDELSI+GH+FEARLYAEDVPKGFLPATGTLTHL F PACRADSG R+GDTISPFYDPMIAKV+VHG TR++AL+RLR AL    V GTVTNLAFLGALA HEGF  G+VDTGLIARDIDALT  P + PRH ALAGMAAL LL     AGFTLW+PL + V L W   +HLL+V+V   D Q W V    V A+RIGGRW VGG  AA V V+   +TVFDGYGLVF  IDPL +AAG  GDGNLIEAPMPGLVR V A VGQ VTKGD+LAVLEAMKMEHSLLAARDGVVAEVLA AGDQVEAG ALVRLE
Sbjct:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADRAAKHVAMADTAVHIGGSAPADSYLRGEVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLVFIGPSAKAIRAMGLKDAAKALMVEAGVPVVPGYHGADQDDALLAAEADKIGYPVLIKAVAGGGGKGMRLVEAADGFQAALESARSEAKTAFGNADVLVEKFVTKPRHIEVQVFGDGKKAVHLFERDCSLQRRHQKVIEEAPAPGMTPEMREAMGQAAVTAAEAIGYAGAGTIEFIVDGSGGLQTDGFFFMEMNTRLQVEHPVTEAITGVDLVAWQLRVAAGEGLPLRQDELSIHGHAFEARLYAEDVPKGFLPATGTLTHLQFTPACRADSGARAGDTISPFYDPMIAKVIVHGDTREIALSRLRAALTGCEVAGTVTNLAFLGALAAHEGFAAGDVDTGLIARDIDALTQAPEVEPRHAALAGMAALSLLDPAEGAGFTLWQPLRRAVGLTWGGEDHLLQVQVVSADHQIWTVDGTEVVAQRIGGRWSVGGQQAAAVSVAEGRVTVFDGYGLVFDVIDPLARAAGGHGDGNLIEAPMPGLVRVVDAKVGQTVTKGDRLAVLEAMKMEHSLLAARDGVVAEVLAQAGDQVEAGVALVRLE 641          

BLAST of 3-methylcrotonyl- carboxylase vs. nr
Match: gi|1055622725|ref|WP_067263400.1| (acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha [Sulfitobacter sp. HI0054] >gi|1024545873|gb|KZY52669.1| 3-methylcrotonyl-CoA carboxylase [Sulfitobacter sp. HI0054])

HSP 1 Score: 1023.85 bits (2646), Expect = 0.000e+0
Identity = 526/644 (81.68%), Postives = 556/644 (86.34%), Query Frame = 0

Query:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAAAKHVAMADVAVHIGGSTPAQSYLRGDVIIQAALDTGAQAIHPGYGFLSENPDFVDAVEAAGLTFIGPSGAAIRAMGLKDAAKALMVEAGVPVVPGYHGKDQSDDLLAAEADKIGYPVLIKAVAGGGGKGMRLVEAAGGFAEALASARSEAKTAFGNPDVLVEKFVSQPRHIEVQVFGDGKTAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRAAMGQAGVKAAEAIGYKGAGTVEFIVDGSDGLKADGFFFMEMNTRLQVEHPVTEAITGVDLVEWQLRVAAGEDLPKAQDELSINGHSFEARLYAEDVPKGFLPATGTLTHLAFDPACRADSGVRSGDTISPFYDPMIAKVVVHGPTRDVALARLRQALANTRVGGTVTNLAFLGALAGHEGFRKGEVDTGLIARDIDALTTPPVIAPRHVALAGMAALGLLQTGPVAGFTLWEPLMQQVTLAWDDAEHLLKVEVSGPDRQSWHVGDDTVEARRIGGRWQVGGVPAAQVDVSGDTITVFDGYGLVFTRIDPLQQAAGAQGDGNLIEAPMPGLVREVFATVGQAVTKGDKLAVLEAMKMEHSLLAARDGVVAEVLAAAGDQVEAGAALVRLEATE 644
            MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADA AKHVAMAD AVHIGGS PA+SYL+GDVIIQAA DTGAQAIHPGYGFLSENPDFV+AVEAAGLTFIGPS  AIRAMGLKDAAKALMVEAGVPVVPGYHG DQ D LLA EA+KIGYPVLIKAVAGGGGKGMRLVE AGGF  AL SARSEAKTAFGNP VLVEKFV++PRHIEVQVFGDG  AVHLFERDCSLQRRHQKVIEEAPAPGMTEEMR AMGQA V+AAEAIGY GAGTVEFIVDGS GL  DGFFFMEMNTRLQVEHPVTE +TGVDLVEWQLRVAAGE LPK QDEL+I+GH+FEARLYAEDVPKGFLPATGTLTHL+F P  RADSGVR+GDTISPFYDPMIAKV+VHG TRDVAL+R+R AL    VGGTVTNLAFLGALA HEGF KGEVDTGLIARDI+ALT  P +  RHVALAGMAALGL Q    AGF LW PL  QV L  +  EHLLKV V   D Q W VGD  V A RI GRW++ G  A  V V+GD +TVFD YG+ F  IDPL +AA A GDGN++EAPMPGLVR + A VGQ+V KGD+LAVLEAMKMEHSLLAARDGVVAEVLA AGDQVEAGAALVRLEA E
Sbjct:    1 MFDTILIANRGEIACRVMETAQAMGVRCVAVYSDADAGAKHVAMADAAVHIGGSAPAESYLKGDVIIQAARDTGAQAIHPGYGFLSENPDFVEAVEAAGLTFIGPSAQAIRAMGLKDAAKALMVEAGVPVVPGYHGADQDDALLAREAEKIGYPVLIKAVAGGGGKGMRLVEEAGGFQAALESARSEAKTAFGNPGVLVEKFVTKPRHIEVQVFGDGTHAVHLFERDCSLQRRHQKVIEEAPAPGMTEEMRDAMGQAAVRAAEAIGYAGAGTVEFIVDGSGGLSTDGFFFMEMNTRLQVEHPVTELVTGVDLVEWQLRVAAGEALPKRQDELTIDGHAFEARLYAEDVPKGFLPATGTLTHLSFTPQTRADSGVRAGDTISPFYDPMIAKVIVHGSTRDVALSRMRAALKGCEVGGTVTNLAFLGALAEHEGFAKGEVDTGLIARDIEALTAQPEVPGRHVALAGMAALGLDQPQAEAGFNLWRPLRHQVMLGREGEEHLLKVRVLSEDVQLWTVGDHEVRAERIAGRWKIDGHIAPAVSVAGDVVTVFDAYGIAFDVIDPLARAAAAHGDGNIVEAPMPGLVRAIDAEVGQSVAKGDRLAVLEAMKMEHSLLAARDGVVAEVLAEAGDQVEAGAALVRLEAEE 644