B F21

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General

End Date: 

Wednesday, April 18, 2012 - 12:00

Experiment Description: 

<p>Knock-down of a carboxypeptidase to see possible effects on molting to adult lice</p>

Experiment Batch ID: 

B

Start Date: 

Monday, March 19, 2012 - 11:00

Experiment Contact: 

Christiane Eichner
Sample

Developmental Stage CV: 

PreAdult2

Number of Individuals (Start): 

30

Sex: 

female

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

LsCP1

Primary target ID: 

EMLSAT00000000705, EMLSAT00000000705-696552 (mRNA) Lepeophtheirus salmonis
Fragment description

Fragment Producer Contact: 

Heidi Kongshaug

Antisense sequence: 

ccaagtgcatcatatgcccaatccgtactccctcctccaactgttgttagaattttggacacgggaccataaaagaacacttcatctgtggctttgcgtatagctcgagatcctttttgcatgatgcgctgatggtcatcaaaatcgtttggttttccttttgataggcataaggtgacattatgagttgagagtaggagtggattgtgaagaaggccgccatttttgtgcctagaggagctaagaaatcacgaacggctgaagtttcttcttcagagaatgcctttgatccatgatatgtatctaagcaaggattggagctgctgcctactgtattccaatctgaaatggatacatcattttttgaagaataaacatttaaaaacagggtcaatagaaacttacgactttcaaagtttctgttcaaatcaactccggtacattttttgttactcttcaatttgaatctatcaaaaagtccagctatgctattttctttttgcacagtattcttttgtgggttccgattctttctccataagcgggagttctcccaggaatagacgtaaccatccggattaaaaacaggaagaatgtaaaagtcaaagttattcattaactccggcctaaaaggggggaaaagtcaattgattttatgatgaattagatatgtcttacgaaacagtgactatacgatctaatgtatacaagcaaaatgctggagaaatccattctcgcgcgtgaataccacaatcca

Fragment Label ID: 

21

Fragment concentration: 

600.00ng/ul

Fragment length: 

757bp

Sense sequence: 

TGGATTGTGGTATTCACGCGCGAGAATGGATTTCTCCAGCATTTTGCTTGTATACATTAGATCGTATAGTCACTGTTTCGTAAGACATATCTAATTCATCATAAAATCAATTGACTTTTCCCCCCTTTTAGGCCGGAGTTAATGAATAACTTTGACTTTTACATTCTTCCTGTTTTTAATCCGGATGGTTACGTCTATTCCTGGGAGAACTCCCGCTTATGGAGAAAGAATCGGAACCCACAAAAGAATACTGTGCAAAAAGAAAATAGCATAGCTGGACTTTTTGATAGATTCAAATTGAAGAGTAACAAAAAATGTACCGGAGTTGATTTGAACAGAAACTTTGAAAGTCGTAAGTTTCTATTGACCCTGTTTTTAAATGTTTATTCTTCAAAAAATGATGTATCCATTTCAGATTGGAATACAGTAGGCAGCAGCTCCAATCCTTGCTTAGATACATATCATGGATCAAAGGCATTCTCTGAAGAAGAAACTTCAGCCGTTCGTGATTTCTTAGCTCCTCTAGGCACAAAAATGGCGGCCTTCTTCACAATCCACTCCTACTCTCAACTCATAATGTCACCTTATGCCTATCAAAAGGAAAACCAAACGATTTTGATGACCATCAGCGCATCATGCAAAAAGGATCTCGAGCTATACGCAAAGCCACAGATGAAGTGTTCTTTTATGGTCCCGTGTCCAAAATTCTAACAACAGTTGGAGGAGGGAGTACGGATTGGGCATATGATGCACTTGG

Fragment Producer: 

Heidi

Primer Group: 

b594
Validation

Efficacy of Knock Down: 

F>80.00%
Phenotype

Phenotype: 

no visible phenotype

Number of Individuals (End): 

21

Gallery Image: 

Image file: 

Image icon Rack5a
Image icon Rack5b
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Image Quality: 

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no visible phenotype, normal development, normal egg development, no significant loss