[UPDATE] No option to extract spliced mRNA or CDS sequence from GBrowse or Tripal

It was noticed that it is not an easy task to extract the spliced mRNA sequence or CDS sequence directly from either GBrowse or Tripal. The annotated sequence of a Gene in Tripal will show the whole gene sequence, as well as the GBrowse CDS track: The CDS track details does not show only the coding sequence but the whole genomic sequence. 

It could be very useful to have an option to easily export only the coding part or only the mature mRNA sequence for designing primers. While it is possible to export a GFF file of the gene model plus the FASTA sequence of the whole chromosome and use an external genome viewer, I assume it might be a good idea to have this feature directly inside GBrowse or Tripal. We are aware of this requirement now and are looking into a solution. I have posted a more detailes question and solution on BioStars https://www.biostars.org/p/135429/

It seems that, until now, there hasn't been a good solution for this in GMOD. Now you can export spliced CDS from GBrowse. Export of the full mRNA, or UTR only, introns only, etc. is not yet implemented, but I am looking into it. 

I have made a quick fix to export the spliced CDS for a gene using GBrowse.

  • Open GBrowse and navigate to the region of interest containing one or many genes
  • Make sure the Ensembl Genes track is open.
  • From the PlugIns menu choose: Download Spliced Coding Sequence File 
  • Press Configure to choose file type or simply press GO
  • CDS sequences open in new window or as download, all sequences in the active view range will be exported