Mucin-like spermatophore wall protein 2 (AG F270)

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General

End Date: 

Friday, September 23, 2016 - 14:50

Experiment Description: 

knockdown in males, checking female phenotype

Experiment Batch ID: 

AG

Start Date: 

Tuesday, August 16, 2016 - 14:42

Experiment Contact: 

Andreas Borchel

Publication: 

A novel gene-family involved in spermatophore generation in the economically important salmon louse Lepeophtheirus salmonis
Sample

Developmental Stage CV: 

adult male

Sex: 

male

Organism: 

Lepeophtheirus salmonis
Target description

Gene or target symbol: 

MLSWP2

Primary target ID: 

EMLSAT00000000183, EMLSAT00000000183-696030 (mRNA) Lepeophtheirus salmonis
Fragment description

Antisense sequence: 

CCAGGCTTTTCACACGCTTT

Fragment Label ID: 

F270

Fragment concentration: 

600.00ng/ul

Fragment length: 

497bp

Sense sequence: 

GCTCTTGGCCGAGCATAAAA
Validation

Efficacy of Knock Down: 

F>80.00%
Phenotype

Phenotype: 

females missing eggstrings/producing no off-spring

Gallery Image: 

Image file: 

Image icon foto-anti183-licebase.png
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Image icon foto-anti183-licebase.png2.06 MB
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Gene 183 is male-specific expressed, a role in reproduction is assumed. Test if knockdown in males affects their reproductive success. It obviously does.