BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. L. salmonis genes
Match: EMLSAG00000009932 (supercontig:LSalAtl2s:LSalAtl2s649:125616:141167:-1 gene:EMLSAG00000009932 transcript:EMLSAT00000009932 description:"maker-LSalAtl2s649-snap-gene-1.20")

HSP 1 Score: 920.228 bits (2377), Expect = 0.000e+0
Identity = 525/1203 (43.64%), Postives = 686/1203 (57.02%), Query Frame = 0

Query:   39 VNMPGAQPKGTDAYLCTAFAVAD---LPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAVD--RARYLVLQVHYVNPLTTPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRARFLTCMDAHSTKIQENMPLDSDVPLPPNPLLEEHAKHSSSNSPHTGTHMESTSIRKGDLDNPLSAKGVDQELSVGGRRKGDLEAPEMNQALERVKTMDIQMNGAKPNQNDDYLCQAFPIKKL---LKAKTR--VYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQ-SKIMYAWAKDAPPTQLPQNVGFSLDGNDGYLVLQVHYKHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHT--PMTLFAFRTHTHTLGKVVTGF--------KVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQDESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTSGNSLEITSFIPSKIKKQDTFNRMGKDI-DQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVS--VLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINS-GQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLRSFPAVPDDPASSGSNRLNPATKLAKESL-KRFENI---IPTTGVGATVAILSLVLTCLLSLCLTCRRCRKRGKGAMNGGTKKKGDGKLDLGDFLGRSKDGFQPLKQDDDEDETGNLSSESDDLEEFSVPALRA 1212
            + MPGA+PKGTD+YLC+ F V     +   +   YVT+FE   A A +AHH LLYAC +PP+ +   +WDCRHH++C D ++ M+AWAKNAE   MPP+VSF +D    ++LVLQVHY + L   D+TG+   +Q + TKYS GI+LL RS+L IPP T  VHG++NC+V + +PLH FA+RTHAH LG VI+GY YS E + + EIARGNPQWPQ FYP+                                                  A  + C D  +  I + +P DSD PLPPNP LE  A H  + S     H       +G LD               G  + DLE        +  K   I+M GA PN  DDY C A+    L   +K+K R  V+I G+  +A +DKAHH+I+  CK P  K+GEI+ CL H MC  + SKIMYAWAKDAP   LP + GF++ G D Y+VLQVHYKHPL++ D T L + YT  +P+  AG+ L+ R+ L IPP      GD+NC + T  PM LFA+RTH HTLG V+TG+        K N     E ARGDPQ PQTFYPM+ +  V  GD L AR                                     F +C +E+  K+   +P +S   + E                              P  I +Q      GK + D  L+PVPNWPPK T D  GQLSGV++D++GN ++FHRGDR WN +TFK NNEYNGD++KPI  NT+LT +++G+IIN+WGA++FF+PHM+T+D QNN+WVTDVA+HQ                     F PG+D  H+CKPT+VAV++DG  F+VADGYCNSRIIKYQ++VD +GYH VS  V+  WG+ANGAG+S + + YAFNIPHGLTLA DK L+CVADRENGR+QCFN  +G FVKS KP   GS++Y + Y P  GG + A+ G EPF  I G   + YV+DIN+  +V+S F  +      PHD+AVS DGS VY +EL P+K+ K T+GK +      + P +P  P  +     +  T+ AK    K  EN+   +P T +    ++LS +LT      L CR    R K   +    KK    LDLGDFLGRSK GF  LK D+ ++E    SSE  DLE+F+VPAL A
Sbjct:   26 IRMPGAKPKGTDSYLCSGFKVEKTFAINNKSDSVYVTKFE-GLAVASKAHHXLLYACXEPPKEE---IWDCRHHSICHDYTTIMFAWAKNAEPLSMPPEVSFKLDPKEHKFLVLQVHYAHELPELDNTGIHFRFQREETKYSGGIYLLARSFLKIPPGTPVVHGEMNCKVKSNIPLHPFAFRTHAHSLGVVITGYKYSPEKNTFIEIARGNPQWPQTFYPIS-------------------------------------------------ADTIYCTDEQNPAITKIIPKDSDTPLPPNPKLEHIALHDKNTSTTKHGH-------RGHLD---------------GELEEDLE--------KNYKLSSIKMPGAIPNMVDDYRCVAYKASALFPEIKSKNRGMVHIMGFLTDAKSDKAHHLIVSHCKVPRGKEGEIFQCLXHGMCSSKDSKIMYAWAKDAPGMNLPPDAGFTVQG-DEYVVLQVHYKHPLQNPDHTSLQLKYTLNKPSFKAGMVLLLRTSLTIPPHTLKTSGDVNCRIKTEDPMYLFAYRTHAHTLGSVITGYQYSFDTKTKKNSLNPLEIARGDPQFPQTFYPMENYVEVEDGDILAARY------------------------------------FIICYDEQEGKLE--VPKDSSDKIHE------------------------------PKNITRQ------GKTLYDSDLHPVPNWPPKATSDIFGQLSGVSLDVHGNAVIFHRGDRKWNADTFKRNNEYNGDRTKPIPQNTVLTLNSNGDIINAWGADMFFMPHMITIDSQNNVWVTDVALHQ---------------------FEPGSDKLHFCKPTSVAVTEDGRNFFVADGYCNSRIIKYQVRVDSNGYHQVSVDVILHWGEANGAGISSSLNNYAFNIPHGLTLAEDKGLICVADRENGRVQCFNILNGVFVKSYKPKVFGSSIYSVAYTPSNGGNLMALGGYEPFSFINGNKPQVYVLDINNENKVLSAFGKD--VLQNPHDLAVSKDGSNVYTIELDPYKLTKFTNGKLLENNGPDAPPNIP--PTETFETTPSSLTEHAKAVFNKHIENLGSYVPKTYITIVASVLSALLT------LICRNM-SRNKVQNSSWKNKKS---LDLGDFLGRSKSGFTRLKTDEHDEEA---SSEDSDLEDFTVPALNA 1032          

HSP 2 Score: 205.682 bits (522), Expect = 2.966e-54
Identity = 108/244 (44.26%), Postives = 151/244 (61.89%), Query Frame = 0

Query:   38 TVNMPGAQPKGTDAYLCTAF-AVADLPVAAHP----FYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDR-SSTMYAWAKNAEATVMPPDVSFAVDRARYLVLQVHYVNPLTTPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETD------NYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAAR 269
            ++ MPGA P   D Y C A+ A A  P          ++  F  + A++++AHH+++  C   P+ + G+++ C  H +C  + S  MYAWAK+A    +PPD  F V    Y+VLQVHY +PL  PDHT L+L Y      + AG+ LLLR+ L+IPP+T K  GD+NC++ T  P++LFAYRTHAH LGSVI+GY YS +T       N  EIARG+PQ+PQ FYPM+    ++ G+ LAAR
Sbjct:  312 SIKMPGAIPNMVDDYRCVAYKASALFPEIKSKNRGMVHIMGFLTD-AKSDKAHHLIVSHCK-VPRGKEGEIFQCLXHGMCSSKDSKIMYAWAKDAPGMNLPPDAGFTVQGDEYVVLQVHYKHPLQNPDHTSLQLKYTLNKPSFKAGMVLLLRTSLTIPPHTLKTSGDVNCRIKTEDPMYLFAYRTHAHTLGSVITGYQYSFDTKTKKNSLNPLEIARGDPQFPQTFYPMENYVEVEDGDILAAR 553          

HSP 3 Score: 204.142 bits (518), Expect = 8.238e-54
Identity = 102/223 (45.74%), Postives = 141/223 (63.23%), Query Frame = 0

Query:  400 KTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTR---VYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGND-GYLVLQVHYKHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHT--PMTLFAFRTHTHTLGKVVTGFKVNEGK--IHEFARGDPQKPQTFYPM 614
            + ++I+M GAKP   D YLC  F ++K      +   VY+T ++  A A KAHH +L  C +P K+  EIW+C HH++C D + IM+AWAK+A P  +P  V F LD  +  +LVLQVHY H L   D TG+   +  E      GIYL+ RS+L+IPP   +VHG++NC+V +  P+  FAFRTH H+LG V+TG+K +  K    E ARG+PQ PQTFYP+
Sbjct:   22 ENVEIRMPGAKPKGTDSYLCSGFKVEKTFAINNKSDSVYVTKFEGLAVASKAHHXLLYACXEPPKE--EIWDCRHHSICHDYTTIMFAWAKNAEPLSMPPEVSFKLDPKEHKFLVLQVHYAHELPELDNTGIHFRFQREETKYSGGIYLLARSFLKIPPGTPVVHGEMNCKVKSNIPLHPFAFRTHAHSLGVVITGYKYSPEKNTFIEIARGNPQWPQTFYPI 242          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. L. salmonis genes
Match: EMLSAG00000011434 (supercontig:LSalAtl2s:LSalAtl2s78:678330:680167:1 gene:EMLSAG00000011434 transcript:EMLSAT00000011434 description:"maker-LSalAtl2s78-snap-gene-6.13")

HSP 1 Score: 145.591 bits (366), Expect = 2.971e-38
Identity = 91/289 (31.49%), Postives = 147/289 (50.87%), Query Frame = 0

Query:   34 ASAFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTV------------CRDRSSTMYAWAKNAEATVMPPDVSFAVDRA---RYLVLQVHYVN---PLTTPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSA-ETDNYTEIARGNPQWPQAFYPMKQPH-TIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEG 302
            A  F   MP   P   ++YLCT   +++       +++  F+PN +Q   AHHML+Y C +P  T   ++W+C   +             C      +YAWAK A    +PP+V F V +    ++LVLQVHY +    L + D +G+ + +  +     AG+ LL     +  P  +    + +C+V     +H FA+RTH H LG ++SG+  S  +   ++ I + +PQ PQ FY ++  +  I   + +AARCT      +    +GAT+ DEMCN Y+MY+I+G
Sbjct:   22 ADVFPALMPKVIPTREESYLCTPIRLSE----DETYFIIGFKPNASQ-HTAHHMLIYGCTEPGSTN--EVWNCGEMSTXTDSAFEASASPCETEPQIIYAWAKGAPQLELPPEVGFKVGKGTTIKFLVLQVHYAHLDMILPSGDDSGVYIKFTDKYQPKEAGVLLLGTGGFA--PAHSTTFFETSCEVRDDREIHPFAFRTHTHGLGRIVSGWKVSPYQNWTWSLIGKRSPQDPQMFYSVEDKNLIITADDIVAARCTMVN-DRDWEVHVGATNEDEMCNFYIMYWIKG 300          

HSP 2 Score: 145.206 bits (365), Expect = 4.387e-38
Identity = 94/279 (33.69%), Postives = 143/279 (51.25%), Query Frame = 0

Query:  406 MNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTM------------CDDQSKIMYAWAKDAPPTQLPQNVGFSLDGND--GYLVLQVHYKH---PLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTPMTL--FAFRTHTHTLGKVVTGFKVNEGKIHEFA---RGDPQKPQTFYPMK-RFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ 661
            M    P + + YLC   PI+  L      +I G+  NAS   AHHM++  C +P     E+WNC   +             C+ + +I+YAWAK AP  +LP  VGF +       +LVLQVHY H    L   D +G+ + +TD+     AG+ L+        P       + +CEV     +  FAFRTHTH LG++V+G+KV+  +   ++   +  PQ PQ FY ++ +   +   D + ARC+  +  ++  V +GAT+ DEMCN YIMY+ +
Sbjct:   29 MPKVIPTREESYLCT--PIR--LSEDETYFIIGFKPNASQHTAHHMLIYGCTEP-GSTNEVWNCGEMSTXTDSAFEASASPCETEPQIIYAWAKGAPQLELPPEVGFKVGKGTTIKFLVLQVHYAHLDMILPSGDDSGVYIKFTDKYQPKEAGVLLLGTG--GFAPAHSTTFFETSCEVRDDREIHPFAFRTHTHGLGRIVSGWKVSPYQNWTWSLIGKRSPQDPQMFYSVEDKNLIITADDIVAARCTMVN-DRDWEVHVGATNEDEMCNFYIMYWIK 299          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. L. salmonis genes
Match: EMLSAG00000008459 (supercontig:LSalAtl2s:LSalAtl2s51:1311671:1338820:-1 gene:EMLSAG00000008459 transcript:EMLSAT00000008459 description:"maker-LSalAtl2s51-augustus-gene-13.14")

HSP 1 Score: 140.969 bits (354), Expect = 6.997e-35
Identity = 97/322 (30.12%), Postives = 141/322 (43.79%), Query Frame = 0

Query:  757 KIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFD-ASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTI 1077
            K+GQ+SGVA+D    P++F RG   WN+ +F  N  Y      PIE +TI+T    +G I+ SWG +LF++PH + VD   N +VTD  +HQV +F P G   K+P + LG  F PGN    +C+P++VAVSD     +VA GY                         +G               ++I H +TL  D+N+ C+A+R    I+CF+         LK P                                             GQ +  F G   G ++PHD+A+S  G  +Y VE+ P K  K+   + I
Sbjct:  374 KLGQVSGVAVDERNRPVIFQRGPVTWNHKSFDRN--YVLQDKTPIEEDTIMTLHPETGEILRSWGKDLFYMPHGIAVDSLGNTYVTDTGLHQVMRF-PLGS--KEPDLTLGNRFEPGNGRNQFCQPSSVAVSDITGDIFVAXGY-------------------------YGD--------------WDIIHSITLFEDENIFCIANRNGQTIECFST-------GLKRP--------------------------------------------FGQKMMLFEG---GISQPHDLAISQYGDVIYVVEMDPEKTNKVLKFEVI 597          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. L. salmonis genes
Match: EMLSAG00000010788 (supercontig:LSalAtl2s:LSalAtl2s714:233602:236979:-1 gene:EMLSAG00000010788 transcript:EMLSAT00000010788 description:"maker-LSalAtl2s714-snap-gene-2.45")

HSP 1 Score: 126.331 bits (316), Expect = 3.327e-32
Identity = 85/261 (32.57%), Postives = 130/261 (49.81%), Query Frame = 0

Query:   30 SASEASAFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTV-----------CRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTP---DHTGLKLYYQAQVT-KYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATP-VPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCT 271
            ++S+   F++ MP  +P   ++  C    +  L        + QFEPN A  + AHHML+Y C  P   +P  +++C                C   S  +Y+WAKNA   ++P  VSF V   +  ++LVLQVHY +    P   D +G+K+ Y  +   K +A +F   RS     P+ ++   D+ C      V +H FA+R H H LG V+SGY    +   +T + +G+PQ PQ FYP     T++ G+ LA RCT
Sbjct:   19 NSSKTEFFSIQMPYLKPIHEESLYCVTKRLNSL----KEIDIIQFEPN-ANEKTAHHMLIYGCATPGSNKP--LFNCGSMNKIENQYGWPTYPCESGSQIIYSWAKNATHLILPKGVSFKVGGGNEIQWLVLQVHYSSVQYIPKAGDSSGVKIVYTEESQPKTAAVLFTSTRS--GRIPSGSETWMDVACIFKDDDVIIHPFAFRVHTHALGRVVSGYKVENQGKTWTLLGKGDPQLPQMFYPTNNI-TMRKGDTLATRCT 269          

HSP 2 Score: 121.324 bits (303), Expect = 1.611e-30
Identity = 90/281 (32.03%), Postives = 136/281 (48.40%), Query Frame = 0

Query:  387 LEAPEMNQALERVKTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTM-----------CDDQSKIMYAWAKDAPPTQLPQNVGFSLDGND--GYLVLQVHY---KHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTPMTL---FAFRTHTHTLGKVVTGFKV-NEGKIHE-FARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGAT 646
            L+   +N +  + +   IQM   KP   +   C    + K L +   + I  ++ NA+   AHHM++  C  P   +  ++NC                C+  S+I+Y+WAK+A    LP+ V F + G +   +LVLQVHY   ++  +  D +G+ + YT+E     A +        RIP   E    D+ C       +   FAFR HTH LG+VV+G+KV N+GK      +GDPQ PQ FYP     T+  GD L  RC+  +  ++  V IGAT
Sbjct:   13 LQCSTLNSS--KTEFFSIQMPYLKPIHEESLYC----VTKRLNSLKEIDIIQFEPNANEKTAHHMLIYGCATP-GSNKPLFNCGSMNKIENQYGWPTYPCESGSQIIYSWAKNATHLILPKGVSFKVGGGNEIQWLVLQVHYSSVQYIPKAGDSSGVKIVYTEESQPKTAAVLFTSTRSGRIPSGSE-TWMDVACIFKDDDVIIHPFAFRVHTHALGRVVSGYKVENQGKTWTLLGKGDPQLPQMFYPTNNI-TMRKGDTLATRCTMVN-DRDTAVWIGAT 283          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. L. salmonis genes
Match: EMLSAG00000003673 (supercontig:LSalAtl2s:LSalAtl2s19:316283:327032:-1 gene:EMLSAG00000003673 transcript:EMLSAT00000003673 description:"maker-LSalAtl2s19-augustus-gene-3.25")

HSP 1 Score: 122.094 bits (305), Expect = 5.999e-31
Identity = 78/251 (31.08%), Postives = 130/251 (51.79%), Query Frame = 0

Query:   30 SASEASAFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTP---DHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCT-YN 273
            S+    + ++ MP ++P   D+YLCT++ +     A     +  +EP QA  + AHH+L+Y C  P   +P  +++C       ++    Y W      T     VSF V   +  +YLVLQVHY +    P   D +G+ +++  +    +AG+  +  S     P+ ++ H D++C +   + +H FA+R H H+LG V+SGY    + D++T I + NPQ PQ FY  +   T+  G+  A+RCT YN
Sbjct:   16 SSQRKVSISLKMPKSKPNHEDSYLCTSYKLD----ANKEMDIIGYEP-QADEKTAHHILIYGCKTPGSMEP--IYNCG----SMNKMEFSYIWPTYPCGT----GVSFKVGGSNAIQYLVLQVHYASVDYIPEEGDESGVNIHFTDESQPKTAGV--IYTSSGGRIPSGSETHLDVSCALQDDIVIHPFAFRVHTHKLGKVVSGYKVENQGDSWTLIGKENPQLPQMFYETRNT-TLTKGDVFASRCTMYN 248          

HSP 2 Score: 120.553 bits (301), Expect = 1.836e-30
Identity = 87/259 (33.59%), Postives = 134/259 (51.74%), Query Frame = 0

Query:  397 ERVKTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHYK---HPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTPMTL--FAFRTHTHTLGKVVTGFKV-NEGKIHEF-ARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGAT 646
            +R  ++ ++M  +KPN  D YLC ++     L A   + I GY+  A    AHH+++  CK P   +  I+NC       ++ +  Y W    P       V F + G++   YLVLQVHY    +  E  D +G+++H+TDE     AG+ +   S  RIP   E  H D++C +   + +  FAFR HTH LGKVV+G+KV N+G       + +PQ PQ FY   R  T+  GD   +RC+  +  ++  V +G+T
Sbjct:   18 QRKVSISLKMPKSKPNHEDSYLCTSYK----LDANKEMDIIGYEPQADEKTAHHILIYGCKTPGSME-PIYNC----GSMNKMEFSYIW----PTYPCGTGVSFKVGGSNAIQYLVLQVHYASVDYIPEEGDESGVNIHFTDESQPKTAGV-IYTSSGGRIPSGSE-THLDVSCALQDDIVIHPFAFRVHTHKLGKVVSGYKVENQGDSWTLIGKENPQLPQMFYET-RNTTLTKGDVFASRCTMYNF-EDHAVWVGST 259          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. L. salmonis genes
Match: EMLSAG00000005441 (supercontig:LSalAtl2s:LSalAtl2s292:636448:640109:-1 gene:EMLSAG00000005441 transcript:EMLSAT00000005441 description:"maker-LSalAtl2s292-snap-gene-6.61")

HSP 1 Score: 67.3958 bits (163), Expect = 2.002e-11
Identity = 84/330 (25.45%), Postives = 140/330 (42.42%), Query Frame = 0

Query:  414 NDD--YLCQAFPIKKLLKAKTRVYITGY---DANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQS---------KIMYAWAKDAPPTQLPQNVGFSLDG--NDGYLVLQVHYKHPLEHK---DFTGLSMHYTDERPTNFAGIY---LMYRSYLRIPPQKEIVHGDINC-----EVHTP---MTLFAFRTHTHTLG-KVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRF---ETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQDESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPE--EPLSYANNEDE 707
            NDD  Y C +  +   + +K + +I  Y    +  + D  HHM + +C   ++ +  IW+      C D+S         K++ AWA  A   + P+  G +  G     Y++++VH+ +P       D +G+++  T E     AGI    L+Y   + IPP         +C      V  P   + +F  + HTH  G +VVT    N  ++ E  R D      F  ++       V PGDYL   C +++  K      G    +EMC  Y+ YY +   T+ ++C +     + S+  D     L+E   E  +PL   N+  E
Sbjct:  226 NDDTTYWCSSHKLNPSIMSK-KHHIIEYLPRISEGNEDVIHHMEIFQCDASLELEFPIWS----GPCGDKSAPPELQMCKKVLAAWAIGAGSFEYPREAGIAFGGPQTSPYMMMEVHFNNPSRRSGIHDESGMTLIITPELRKYDAGILELGLIYSDLMAIPPYAVNFPLSGHCLPQCTAVGLPEEGIIIFGSQLHTHQTGARVVTKHFRNGEELPEINR-DNHYSTHFQEIRLLHEPRKVFPGDYLKTTCFYDTTHKTNVTLGGFGFEEEMCVNYVHYYPR---TNLEICKS----SVDSMALDKYFQFLNEWNNEDTDPLKSVNDNYE 542          

HSP 2 Score: 66.6254 bits (161), Expect = 3.422e-11
Identity = 67/289 (23.18%), Postives = 122/289 (42.21%), Query Frame = 0

Query:   51 AYLCTAFAVADLPVAAHPFYVTQFEP--NQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSS---------TMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTP---DHTGLKLYYQAQVTKYSAGIF---LLLRSYLSIPPNTAKVHGDINCQ---VATPVP---LHLFAYRTHAHQLGS-VISGYIYSAETDNYTEIARGN--PQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRARFLTC 310
             Y C++  + +  + +   ++ ++ P  ++   +  HHM ++ C+   + +   +W       C D+S+          + AWA  A +   P +   A      + Y++++VH+ NP       D +G+ L    ++ KY AGI    L+    ++IPP         +C     A  +P   + +F  + H HQ G+ V++ +  + E     EI R N      Q    + +P  + PG++L   C Y+T      T  G    +EMC  Y+ YY   PR     C
Sbjct:  230 TYWCSSHKL-NPSIMSKKHHIIEYLPRISEGNEDVIHHMEIFQCDASLELE-FPIWS----GPCGDKSAPPELQMCKKVLAAWAIGAGSFEYPREAGIAFGGPQTSPYMMMEVHFNNPSRRSGIHDESGMTLIITPELRKYDAGILELGLIYSDLMAIPPYAVNFPLSGHCLPQCTAVGLPEEGIIIFGSQLHTHQTGARVVTKHFRNGE--ELPEINRDNHYSTHFQEIRLLHEPRKVFPGDYLKTTCFYDTTHKTNVTLGGFGFEEEMCVNYVHYY---PRTNLEIC 507          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. SwissProt
Match: gi|113696|sp|P12890.1|AMDB_XENLA (RecName: Full=Peptidyl-glycine alpha-amidating monooxygenase B; Short=PAM-B; AltName: Full=Peptide C-terminal alpha-amidating enzyme II; Short=AE-II; AltName: Full=Peptidyl-glycine alpha-amidating monooxygenase II; Includes: RecName: Full=Peptidylglycine alpha-hydroxylating monooxygenase B; Short=PHM-B; Includes: RecName: Full=Peptidyl-alpha-hydroxyglycine alpha-amidating lyase B; AltName: Full=Peptidylamidoglycolate lyase-B; Short=PAL-B; Flags: Precursor)

HSP 1 Score: 489.189 bits (1258), Expect = 7.547e-155
Identity = 305/843 (36.18%), Postives = 448/843 (53.14%), Query Frame = 0

Query:  401 TMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHY----KHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTP-MTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTSGNSLEITSFIPSKIKKQDTFNRMGKDIDQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDA-SGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLRSFPAVPDDPASSGSN---RLNPAT-KLAKESLKRFENIIPTTGVGA------TVAILSLVLTCLLSLCLTCRRCRKRG----------KGAMNGGTKKKGDGKLDLGDFL----GRSKDGFQPLKQDDDEDETGNLSSESDDLEEFSVPAL 1210
            T+DI+M G  P ++D YLC+++     L      Y+  Y  +A+ D AHHM+L  C  P   D + W+C   T C+D+S IMYAWAK+APPT+LP+ VGF + G  G  Y VLQVHY        +HKD TG+++  T E+    AGIYL       +PP +E+V+ DI C  + P +  FA+R HTH LG+VV+GF+V  GK     R  PQ PQ FYP++    ++PGD +  RC F    +     IG T+ DEMCNLYIMYY     +T +  C      K+   IP+ ++ P+  S   + +           T   +   T+    K ++++  N+     D  L    +WP      K+GQ+SG+A+D   N ++FHRGD +W+ N+F  N  Y      PI+ +TIL  D  +  ++ S G NLFFLPH LT+D+  N WVTDVA+HQVFK    G   + PL+VLG+ F PG+D +H+C+PT VAV      F+VADGYCNSRI+++          N   + QWG+   + L     P  F IPH LT+ +D+  LCVADRENGRIQCF+A +G+FVK +K  E G  V+ + YAP  GG +YAV G   +G  T    +G++++ ++G ++ TF    + F  PHD+A   DG TVY  +     VWK +  K    +  ++   V +   +       R  P T +   +  +   +++  +  G       T+ I+ +V+   +++ +  R+ R  G           G + G  + KG G L+LG F     G S+ GF  L  +  + E  +    SD  EE+S P +
Sbjct:   61 TLDIRMPGVTPTESDTYLCKSY----RLPVDDEAYVVDYRPHANMDTAHHMLLFGCNVPSSTD-DYWDCSAGT-CNDKSSIMYAWAKNAPPTKLPEGVGFQVGGKSGSRYFVLQVHYGDVKAFQDKHKDCTGVTVRITPEKQPLIAGIYLSMSLNTVVPPGQEVVNSDIACLYNRPTIHPFAYRVHTHQLGQVVSGFRVRHGKWTLIGRQSPQLPQAFYPVEHPLEISPGDIIATRCLFTGKGRMSATYIGGTAKDEMCNLYIMYYMDAAHATSYMTCVQTGNPKLFENIPEIANVPIPVS--PDMMMMMMMGHGHHHTEAEAETNTALQQPKREEEEVLNQ-----DVHLEEDTDWPGVNL--KVGQVSGLALDPKNNLVIFHRGDHVWDENSFDRNFVYQQRGIGPIQESTILVVDPNTSKVLKSTGQNLFFLPHGLTIDRDGNYWVTDVALHQVFKV---GAEKETPLLVLGRAFQPGSDRKHFCQPTDVAVDPITGNFFVADGYCNSRIMQFS--------PNGMFIMQWGEETSSNLP---RPGQFRIPHSLTMISDQGQLCVADRENGRIQCFHAKTGEFVKQIKHQEFGREVFAVSYAP--GGVLYAVNGKPYYGDSTP--VQGFMLNFSNGDILDTFIPARKNFEMPHDIAAGDDG-TVYVGDAHANAVWKFSPSKAEHRSVKKAGIEVEEITETEIFETHMRSRPKTNESVGQQTQEKPSVVQESSAGVSFVLIITLLIIPVVVLIAIAIFIRWRKVRMYGGDIGHKSESSSGGILGKLRGKGSGGLNLGTFFATHKGYSRKGFDRLSTEGSDQEKDDDDDGSDSEEEYSAPPI 869          

HSP 2 Score: 219.935 bits (559), Expect = 6.352e-58
Identity = 129/322 (40.06%), Postives = 174/322 (54.04%), Query Frame = 0

Query:   24 MGRADPSASEASA---FTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTPDH----TGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRA-RFLTCMDAHSTKIQENMPLDSDVPLPPNP 334
            +G   P  S  S+     + MPG  P  +D YLC ++    LPV     YV  + P+ A  + AHHMLL+ CN P  T     WDC   T C D+SS MYAWAKNA  T +P  V F V     +RY VLQVHY +     D     TG+ +    +     AGI+L +     +PP    V+ DI C    P  +H FAYR H HQLG V+SG+        +T I R +PQ PQAFYP++ P  I PG+ +A RC +   G    T IG T+ DEMCNLY+MYY++   A  ++TC+   + K+ EN+P  ++VP+P +P
Sbjct:   46 LGTTRPVMSPGSSDYTLDIRMPGVTPTESDTYLCKSYR---LPVDDEA-YVVDYRPH-ANMDTAHHMLLFGCNVPSSTD--DYWDCSAGT-CNDKSSIMYAWAKNAPPTKLPEGVGFQVGGKSGSRYFVLQVHYGDVKAFQDKHKDCTGVTVRITPEKQPLIAGIYLSMSLNTVVPPGQEVVNSDIACLYNRPT-IHPFAYRVHTHQLGQVVSGF--RVRHGKWTLIGRQSPQLPQAFYPVEHPLEISPGDIIATRCLFTGKGRMSATYIGGTAKDEMCNLYIMYYMDAAHATSYMTCVQTGNPKLFENIPEIANVPIPVSP 356          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. SwissProt
Match: gi|292495081|sp|P08478.3|AMDA_XENLA (RecName: Full=Peptidyl-glycine alpha-amidating monooxygenase A; Short=PAM-A; AltName: Full=Peptide C-terminal alpha-amidating enzyme I; Short=AE-I; AltName: Full=Peptidyl-glycine alpha-amidating monooxygenase I; Includes: RecName: Full=Peptidylglycine alpha-hydroxylating monooxygenase A; Short=PHM-A; Includes: RecName: Full=Peptidyl-alpha-hydroxyglycine alpha-amidating lyase A; AltName: Full=Peptidylamidoglycolate lyase-A; Short=PAL-A; Flags: Precursor)

HSP 1 Score: 483.411 bits (1243), Expect = 6.169e-152
Identity = 276/685 (40.29%), Postives = 389/685 (56.79%), Query Frame = 0

Query:  401 TMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHYKHPL----EHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTP-MTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPDNSDTPLDES-QPEEPLSYANNEDEREPTSGNSLEITSFIPSKIKKQDTFNRMGKDIDQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFD-ASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGK 1075
            T+DI+M G  P ++D YLC+++     L      Y+  +  +A+ D AHHM+L  C  P   D + W+C   T C D+S IMYAWAK+APPT+LP+ VGF + G  G  Y VLQVHY +      +HKD TG+++  T E+    AGIYL       IPP +E V+ DI C  + P +  FA+R HTH LG+VV+GF+V  GK     R  PQ PQ FYP++    ++PGD +  RC F    +     IG TS DEMCNLYIMYY     +T +  C      K+   IP+ ++ P+  S      + + ++  E EP     L+       ++  QD    + +D D        WP      K+GQ+SG+A+D   N  +FHRGD +W+ N+F  N  Y      PI+ +TIL  D +S  ++ S G NLFFLPH LT+D+  N WVTDVA+HQVFK    G   + PL+VLG+ F PG+D +H+C+PT VAV      F+VADGYCNSRI+++          N   + QWG+   + +     P  F IPH LT+  D+  LCVADRENGRIQCF+A++G FVK +K  E G  V+ + YAP  GG +YAV G +P+   +  V +G++++ ++G ++ TF    + F+ PHD+A + DG TVY  +     VWK +  K
Sbjct:   59 TLDIRMPGVTPTESDTYLCKSY----RLPVDDEAYVVDFRPHANMDTAHHMLLFGCNIPSSTD-DYWDCSAGT-CMDKSSIMYAWAKNAPPTKLPEGVGFRVGGKSGSRYFVLQVHYGNVKAFQDKHKDCTGVTVRVTPEKQPQIAGIYLSMSVDTVIPPGEEAVNSDIACLYNRPTIHPFAYRVHTHQLGQVVSGFRVRHGKWSLIGRQSPQLPQAFYPVEHPVEISPGDIIATRCLFTGKGRTSATYIGGTSNDEMCNLYIMYYMDAAHATSYMTCVQTGEPKLFQNIPEIANVPIPVSPDMMMMMGHGHHHTEAEPEKNTGLQQPKREEEEVLDQDV--HLEEDTD--------WPGVNL--KVGQVSGLALDPKNNLAIFHRGDHVWDENSFDRNFVYQQRGIGPIQESTILVVDPSSSKVLKSTGKNLFFLPHGLTIDRDGNYWVTDVALHQVFKL---GAGKETPLLVLGRAFQPGSDRKHFCQPTDVAVDPITGNFFVADGYCNSRIMQFS--------PNGMFIMQWGEETSSNVP---RPGQFRIPHSLTMVPDQGQLCVADRENGRIQCFHAETGNFVKQIKHQEFGREVFAVSYAP--GGVLYAVNG-KPYYGYSAPV-QGFMLNFSNGDILDTFIPARKNFDMPHDIAAADDG-TVYVGDAHANAVWKFSPSK 706          

HSP 2 Score: 223.016 bits (567), Expect = 1.265e-58
Identity = 131/322 (40.68%), Postives = 173/322 (53.73%), Query Frame = 0

Query:   24 MGRADPSASEASA---FTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTPDH----TGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRA-RFLTCMDAHSTKIQENMPLDSDVPLPPNP 334
            +G   P  S  S+     + MPG  P  +D YLC ++    LPV     YV  F P+ A  + AHHMLL+ CN P  T     WDC   T C D+SS MYAWAKNA  T +P  V F V     +RY VLQVHY N     D     TG+ +    +     AGI+L +     IPP    V+ DI C    P  +H FAYR H HQLG V+SG+        ++ I R +PQ PQAFYP++ P  I PG+ +A RC +   G    T IG TS DEMCNLY+MYY++   A  ++TC+     K+ +N+P  ++VP+P +P
Sbjct:   44 LGTTRPVMSPGSSDYTLDIRMPGVTPTESDTYLCKSYR---LPVDDEA-YVVDFRPH-ANMDTAHHMLLFGCNIPSSTD--DYWDCSAGT-CMDKSSIMYAWAKNAPPTKLPEGVGFRVGGKSGSRYFVLQVHYGNVKAFQDKHKDCTGVTVRVTPEKQPQIAGIYLSMSVDTVIPPGEEAVNSDIACLYNRPT-IHPFAYRVHTHQLGQVVSGF--RVRHGKWSLIGRQSPQLPQAFYPVEHPVEISPGDIIATRCLFTGKGRTSATYIGGTSNDEMCNLYIMYYMDAAHATSYMTCVQTGEPKLFQNIPEIANVPIPVSP 354          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. SwissProt
Match: gi|74867287|sp|Q9V5E1.1|PAL1_DROME (RecName: Full=Peptidyl-alpha-hydroxyglycine alpha-amidating lyase 1; AltName: Full=Peptidylamidoglycolate lyase 1; AltName: Full=dPAL1; Flags: Precursor)

HSP 1 Score: 291.582 bits (745), Expect = 2.159e-85
Identity = 177/467 (37.90%), Postives = 248/467 (53.10%), Query Frame = 0

Query:  749 WPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFD-ASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYA-FNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGK---VTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLRSFPAVPDDPASS--GSNRLNPATKLAKESLKRFENIIPTTGVGATVAILSLVLT---CLLSLCLTCRRCRKRG------KGAMNGGTKKKGDGKLDLGDFLGRSKDGFQPLKQ--DDDEDETGNLSS 1197
            WP      K+G ++ V+ D  GN ++FHR +R+W   TF + N+Y      PI  +TIL  + A+G +   WG N F++PH LTVD ++N+W+TDVAMHQVFKF P GG D KP + LG  F PG+  R +CKPT+VAV D+G+ F+VADGYCN+RI+KY  K +        ++  WGQ   +G+S   +P   F IPH LTL  +  LLC ADRENGR+QCF + +G F        IG  ++ + Y P  GG++  V GP     I  +      G+V+ + S Q+VS F      F  PHDVAV+ADG+ +Y  EL P ++ K        P +L +     D   S   G +++ PA  +   S             G  + + SL+L       +L L   R RKRG      +G  +   K  G     LG  L R ++GF+ L Q   D+E ET  L+S
Sbjct:  101 WPANNV--KLGAVTAVSFDKAGNVVIFHRVNRVWGQTTFDNRNQYQEKYRGPIRESTILALEPATGKVQYDWGKNFFYMPHGLTVDPEDNVWLTDVAMHQVFKFPPRGG-DGKPALTLGDAFQPGS-GRKFCKPTSVAVLDNGD-FFVADGYCNARILKYSRKGE--------LILFWGQNTFSGISYDVAPQNFFAIPHALTLVPELQLLCAADRENGRVQCFLSSNGTFHSQYHNQLIGDRLFSMAYTPAAGGQLVIVNGPTAELGIHPEHYNEVHGFVLSMRSKQLVSKFGPNNLQFQNPHDVAVTADGNEIYVAELNPMRIHKFVHRSLAKPMSLSASKDSRDSAISQAVGGDQV-PAVAVHHPS-------------GKAILVASLMLLFAGSTFALALIFARRRKRGCLPFGARGRRHAWEKSDG---FKLGGLLDRDRNGFEKLDQQASDEEQETKTLAS 537          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. SwissProt
Match: gi|114149226|sp|P83388.2|AMDL_CAEEL (RecName: Full=Probable peptidyl-glycine alpha-amidating monooxygenase pamn-1; Short=PAM; Includes: RecName: Full=Probable peptidylglycine alpha-hydroxylating monooxygenase; Short=PHM; Includes: RecName: Full=Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase; AltName: Full=Peptidylamidoglycolate lyase; Short=PAL; Flags: Precursor)

HSP 1 Score: 274.248 bits (700), Expect = 5.321e-78
Identity = 211/678 (31.12%), Postives = 308/678 (45.43%), Query Frame = 0

Query:  399 VKTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDG-NDG--YLVLQVHYKHPL--EHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINC--EVHTPMTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQDESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTSGNSLE---ITSFIPSKIKKQDTFNRMGKDIDQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDASGN---IINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQ-ANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKP---PEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVY 1059
            ++   IQM G  P + DDY+  +          T  Y+  ++  A AD+ HHM+L  C  P  + G  W  +        S I+YAWA++AP   LP++V FS+    DG  Y VLQVHY  P   E  DF+G++MH + ++P N A + +++ S   IPPQ      +I C  E  TP+  FAFRTHTH +G++V+ F  ++G   +  + +PQ PQ F  +     +  GD + A C F+SM KN TV++GA   DEMCN Y+M++                 K+ +  P  +    D   P + + Y  +  E  P S   LE     S +P  I ++     +G                    K+GQ++G+A +     +VF R  R+W+ +TF + N    DK KPI    IL    SGN   +    G   F+LPH + VDK   ++ TDV  H V K+    G + K +   G+   PG+D  HYCKPT +   +D    YV DGYCNSR++   L        N   + Q+G     AG         FN+PH +   +   LL V DRENGR+Q      G  ++  K      I S     DY  +  GR       E      G+   G +               G  F +PH + V  DG  ++
Sbjct:   32 IQKFTIQMIGYSPQKTDDYVAVSI-------EATPGYVVAFEPMAHADRVHHMLLYGCTMPASEQG-FWRGMETCGWGGGSYILYAWARNAPNLVLPKDVAFSVGHEQDGIKYFVLQVHYAQPFAGEVHDFSGVTMHISQKKPMNLAAV-MLFVSGTPIPPQLPAFQNNITCMFESSTPIHPFAFRTHTHAMGRLVSAFFKHDGHWTKIGKRNPQWPQLFEGIPSKLMIGSGDQMSASCRFDSMDKNRTVNMGAMGVDEMCNFYMMFHYD--------------AKLDNPYPQGAICAKD--YPSKMIDYPKDGFELLP-SRPELEHHAHQSKVPFGIVQEAIHENLGG------------------VKLGQVAGLAFNNEQQLLVFQRAGRVWDASTFDNYNILL-DK-KPIADPVILVISYSGNQTKLERKLGGGQFYLPHGIYVDKDGFVYTTDVGSHTVAKWK-IEGNELKNIWTSGELLMPGSDQHHYCKPTGITRVED--QLYVTDGYCNSRVVVLDL--------NGKRIRQFGLPGEDAG--------QFNLPHDIVSDSAGRLL-VTDRENGRVQHMTT-QGHVIEEFKSTMFTNIYSAASHEDYVFMVPGRPIMGHETEGIAVFVGRSGTGLIEYAFGPTTKGKREQMGPQFGQPHCLRVCPDGGHIF 642          

HSP 2 Score: 179.874 bits (455), Expect = 8.381e-46
Identity = 107/317 (33.75%), Postives = 158/317 (49.84%), Query Frame = 0

Query:   37 FTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV----DRARYLVLQVHYVNPLT--TPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYY----IEGPRARFLTCMDAHSTKIQENMPLDSDVPLPPNPLLEEHAKHS 343
            FT+ M G  P+ TD Y+  +       + A P YV  FEP  A A+R HHMLLY C  P   Q    W           S  +YAWA+NA   V+P DV+F+V    D  +Y VLQVHY  P      D +G+ ++   +     A + L + S   IPP       +I C   +  P+H FA+RTH H +G ++S +       ++T+I + NPQWPQ F  +     I  G+ ++A C ++++  N+T  +GA   DEMCN Y+M++    ++ P  +   C   + +K+ +  P D    LP  P LE HA  S
Sbjct:   35 FTIQMIGYSPQKTDDYVAVS-------IEATPGYVVAFEP-MAHADRVHHMLLYGCTMPASEQ--GFWRGMETCGWGGGSYILYAWARNAPNLVLPKDVAFSVGHEQDGIKYFVLQVHYAQPFAGEVHDFSGVTMHISQKKPMNLAAVMLFV-SGTPIPPQLPAFQNNITCMFESSTPIHPFAFRTHTHAMGRLVSAFF--KHDGHWTKIGKRNPQWPQLFEGIPSKLMIGSGDQMSASCRFDSMDKNRTVNMGAMGVDEMCNFYMMFHYDAKLDNPYPQGAICAKDYPSKMID-YPKDGFELLPSRPELEHHAHQS 337          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. SwissProt
Match: gi|113705|sp|P14925.1|AMD_RAT (RecName: Full=Peptidyl-glycine alpha-amidating monooxygenase; Short=PAM; Includes: RecName: Full=Peptidylglycine alpha-hydroxylating monooxygenase; Short=PHM; Includes: RecName: Full=Peptidyl-alpha-hydroxyglycine alpha-amidating lyase; AltName: Full=Peptidylamidoglycolate lyase; Short=PAL; Flags: Precursor)

HSP 1 Score: 264.233 bits (674), Expect = 3.487e-72
Identity = 165/482 (34.23%), Postives = 250/482 (51.87%), Query Frame = 0

Query:  759 GQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDAS-GNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLRSF---------------PAVPDDPASSGSNRLNPATKLAKESLKRFENIIPTTGVGATVAILSLVLTCLLSLCLTCRRCRKRG---------KGAMNGGTKKKGDGKLDLGDFL----GRSKDGFQPLK---QDDDEDETGNLSSESDDLEEFSVP 1208
            GQ+SGVA+D   N ++FHRGD +W+ N+F S   Y      PIE +TIL  D +   I+ S G NLF+LPH L++D   N WVTDVA+HQVFK  P+  + + PL++LG+   PG+D  H+C+PT VAV       +V+DGYCNSRI+++          +   +TQWG+ +      +  P  F++PH L L    + LCVADRENGRIQCF  D+ +FV+ +K    G  V+ + Y P   G ++AV G   FG    +  +G+V++ +SG+++  F    + F+ PHD+  S DG TVY  +     VWK T  + +   +++                 P V + P SS   ++    KL+ E       ++ TT +   V +L  ++     + +  ++ R  G          G + G  + KG G L+LG+F     G S+ GF  +     D ++DE     SE    EE+S P
Sbjct:  515 GQVSGVALDSKNNLVIFHRGDHVWDGNSFDSKFVYQQRGLGPIEEDTILVIDPNNAEILQSSGKNLFYLPHGLSIDTDGNYWVTDVALHQVFKLDPH--SKEGPLLILGRSMQPGSDQNHFCQPTDVAVEPSTGAVFVSDGYCNSRIVQFS--------PSGKFVTQWGEESSGS---SPRPGQFSVPHSLALVPHLDQLCVADRENGRIQCFKTDTKEFVREIKHASFGRNVFAISYIP---GFLFAVNGKPYFG--DQEPVQGFVMNFSSGEIIDVFKPVRKHFDMPHDIVASEDG-TVYIGDAHTNTVWKFTLTEKMEHRSVKKAGIEVQEIKEAEAVVEPKVENKPTSSELQKMQEKQKLSTEPGSGVSVVLITTLLVIPVLVLLAIV-----MFIRWKKSRAFGDHDRKLESSSGRVLGRFRGKGSGGLNLGNFFASRKGYSRKGFDRVSTEGSDQEKDEDDGTESE----EEYSAP 968          

HSP 2 Score: 221.861 bits (564), Expect = 3.474e-58
Identity = 131/361 (36.29%), Postives = 188/361 (52.08%), Query Frame = 0

Query:   24 MGRADPSASEASAFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAVDR---ARYLVLQVHYVNPLTTPDH----TGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRA-RFLTCMDAHSTKIQENMPLDSDVPLPPNP-LLEEHAKHSSSNSPHTGTHM------ESTSIRKGDLDNPLS 369
            +G   P  +   A  + MPG  PK +D Y C +     LPV    F V  F+P +A  +  HHMLL+ CN P  T  G  W C   T C D+++ +YAWA+NA  T +P  V F V     ++Y VLQVHY +     D+    +G+ ++         AG++L++     IPP    V+ DI+CQ     P+H+FAYR H H LG V+SGY        +T I R NPQ PQAFYP++ P  +  G+ LAARC +   G  + T IG TS DEMCNLY+MYY+E   A  F+TC    +  +   +P ++++P+P  P ++  H  H  + +      M      E   + +GD  + LS
Sbjct:   51 IGPVTPLDASDFALDIRMPGVTPKESDTYFCMSMR---LPVDEEAF-VIDFKP-RASMDTVHHMLLFGCNMPSST--GSYWFCDEGT-CTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSVHLTRVPQPLIAGMYLMMSVDTVIPPGEKVVNADISCQYKM-YPMHVFAYRVHTHHLGKVVSGY--RVRNGQWTLIGRQNPQLPQAFYPVEHPVDVTFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKYALSFMTCTKNVAPDMFRTIPAEANIPIPVKPDMVMMHGHHKEAENKEKSALMQQPKQGEEEVLEQGDFYSLLS 400          

HSP 3 Score: 220.32 bits (560), Expect = 1.146e-57
Identity = 119/297 (40.07%), Postives = 164/297 (55.22%), Query Frame = 0

Query:  402 MDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHY----KHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHT-PMTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQDE-STDFQLCGNEEVRKISSLIPDNSDTPL 690
            +DI+M G  P ++D Y C +      L      ++  +   AS D  HHM+L  C  P    G  W C   T C D++ I+YAWA++APPT+LP+ VGF + G  G  Y VLQVHY         HKD +G+S+H T       AG+YLM      IPP +++V+ DI+C+    PM +FA+R HTH LGKVV+G++V  G+     R +PQ PQ FYP++    V  GD L ARC F    + E   IG TS+DEMCNLYIMYY + + +  F  C       +   IP  ++ P+
Sbjct:   64 LDIRMPGVTPKESDTYFCMSM----RLPVDEEAFVIDFKPRASMDTVHHMLLFGCNMP-SSTGSYWFCDEGT-CTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSVHLTRVPQPLIAGMYLMMSVDTVIPPGEKVVNADISCQYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQNPQLPQAFYPVEHPVDVTFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKYALSFMTCTKNVAPDMFRTIPAEANIPI 354          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. SwissProt
Match: gi|341940211|sp|P97467.2|AMD_MOUSE (RecName: Full=Peptidyl-glycine alpha-amidating monooxygenase; Short=PAM; Includes: RecName: Full=Peptidylglycine alpha-hydroxylating monooxygenase; Short=PHM; Includes: RecName: Full=Peptidyl-alpha-hydroxyglycine alpha-amidating lyase; AltName: Full=Peptidylamidoglycolate lyase; Short=PAL; Flags: Precursor)

HSP 1 Score: 260.381 bits (664), Expect = 6.761e-71
Identity = 157/460 (34.13%), Postives = 238/460 (51.74%), Query Frame = 0

Query:  759 GQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDAS-GNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLRS-------FPAVPDDPASSGSNRLNPATKLAKESLKRFENIIPTTGVGATVAILSLVLTCL------LSLCLTCRRCRKRG---------KGAMNGGTKKKGDGKLDLGDFL----GRSKDGFQPLKQDDDEDE 1191
            GQ+SGVA+D   N ++FHRGD +W+ N+F S   Y      PIE +TIL  D +   I+ S G NLF+LPH L++D   N WVTDVA+HQVFK  P   + + PL+VLG+   PG+D  H+C+PT VAV       +V+DGYCNSRI+++          +   +TQWG+ +      +  P  F++PH L L    N LCVADRENGRIQCF  D+ +FV+ +K    G  V+ + Y P   G ++AV G   FG    +  +G+V++ +SG+++  F    + F+ PHD+  S DG TVY  +     VWK T  ++      RS        P + +  A       N  T    + ++  + +I   G G  V +++ +L         +++ +  ++ R  G          G + G  + KG   L+LG+F     G S+ GF  +  +  + E
Sbjct:  515 GQVSGVALDSKNNLVIFHRGDHVWDGNSFDSKFVYQQRGLGPIEEDTILVIDPNKAEILQSSGKNLFYLPHGLSIDTDGNYWVTDVALHQVFKLEPR--SKEGPLLVLGRSMQPGSDQNHFCQPTDVAVEPSTGAVFVSDGYCNSRIVQFS--------PSGKFITQWGEESSGS---SPKPGQFSVPHSLALVPHLNQLCVADRENGRIQCFKTDTKEFVREIKHASFGRNVFAISYIP---GFLFAVNGKPYFG--DQEPVQGFVMNFSSGEIIDVFKPVRKHFDMPHDIVASEDG-TVYIGDAHTNTVWKFTLTESRLEVEHRSVKKAGIEVPEIKEAEAVVEPKVKNKPTSSELQKMQEKKKLIKDPGSGVPVVLITTLLVIPVVVLLAIAMFIRWKKSRAFGDHDRKLESSSGRVLGRLRGKGSSGLNLGNFFASRKGYSRKGFDRVSTEGSDQE 955          

HSP 2 Score: 223.402 bits (568), Expect = 1.220e-58
Identity = 120/298 (40.27%), Postives = 163/298 (54.70%), Query Frame = 0

Query:  401 TMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHY----KHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHT-PMTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPDNSDTPL 690
            T+DI+M G  P ++D Y C +      L      ++  +   AS D  HHM+L  C  P    G  W C   T C D++ I+YAWA++APPT+LP+ VGF + G  G  Y VLQVHY         HKD +G+S+H T       AG+YLM      IPP +++V+ DI+C     PM +FA+R HTH LGKVV+G++V  G+     R  PQ PQ FYP++    V  GD L ARC F    + E   IG TS+DEMCNLYIMYY +   +  F  C       +   IP+ ++ P+
Sbjct:   62 TLDIRMPGVTPKESDTYFCMSM----RLPVDEEAFVIDFKPRASMDTVHHMLLFGCNMP-SSTGSYWFCDEGT-CTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSLHLTRVPQPLIAGMYLMMSVNTVIPPGEKVVNSDISCHYKMYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVEHPVDVAFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPEEANIPI 353          

HSP 3 Score: 214.157 bits (544), Expect = 1.053e-55
Identity = 121/309 (39.16%), Postives = 171/309 (55.34%), Query Frame = 0

Query:   33 EASAFT--VNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAVDR---ARYLVLQVHYVNPLTTPDH----TGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRA-RFLTCMDAHSTKIQENMPLDSDVPLP 331
            ++S FT  + MPG  PK +D Y C +     LPV    F V  F+P +A  +  HHMLL+ CN P  T  G  W C   T C D+++ +YAWA+NA  T +P  V F V     ++Y VLQVHY +     D+    +G+ L+         AG++L++     IPP    V+ DI+C      P+H+FAYR H H LG V+SGY        +T I R +PQ PQAFYP++ P  +  G+ LAARC +   G  + T IG TS DEMCNLY+MYY+E   A  F+TC    +  +   +P ++++P+P
Sbjct:   57 DSSDFTLDIRMPGVTPKESDTYFCMSMR---LPVDEEAF-VIDFKP-RASMDTVHHMLLFGCNMPSST--GSYWFCDEGT-CTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSLHLTRVPQPLIAGMYLMMSVNTVIPPGEKVVNSDISCHYKM-YPMHVFAYRVHTHHLGKVVSGY--RVRNGQWTLIGRQSPQLPQAFYPVEHPVDVAFGDILAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPEEANIPIP 354          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. SwissProt
Match: gi|231532|sp|P10731.2|AMD_BOVIN (RecName: Full=Peptidyl-glycine alpha-amidating monooxygenase; Short=PAM; Includes: RecName: Full=Peptidylglycine alpha-hydroxylating monooxygenase; Short=PHM; Includes: RecName: Full=Peptidyl-alpha-hydroxyglycine alpha-amidating lyase; AltName: Full=Peptidylamidoglycolate lyase; Short=PAL; Flags: Precursor)

HSP 1 Score: 257.299 bits (656), Expect = 7.622e-70
Identity = 142/367 (38.69%), Postives = 205/367 (55.86%), Query Frame = 0

Query:  759 GQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDAS-GNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLRSF---------------PAVPDDPASSGSNRLNPATKLAKE 1109
            GQ+SGVA+D   N ++FHRGD +W+ N+F S   Y      PIE +TIL  D +   ++ S G NLF+LPH L++DK  N WVTDVA+HQVFK  P   + + PL+ LG+   PG+D  H+C+PT VAV  D  T YV+DGYCNSR++++          +   +TQWG+A+   L  +  P  F +PH L L      LCVADRENGRIQCF  D+ +FV+ +K P  G  V+ + Y P   G ++AV G   F     +  +G+V++ +SG+++  F    + F+ PHD+A S DG TVY  +     VWK T  + +   +++                   + + PASS   ++    KL KE
Sbjct:  512 GQVSGVALDPQNNLVIFHRGDHVWDGNSFDSKFVYQQRGLGPIEEDTILVIDPNNAAVLQSSGKNLFYLPHGLSIDKDGNYWVTDVALHQVFKLDP--KSKEGPLLTLGRSMQPGSDQNHFCQPTDVAVDPDTGTIYVSDGYCNSRLVQFS--------PSGKFITQWGEAS---LESSPKPGQFRVPHSLALVPPLGQLCVADRENGRIQCFKTDTKEFVREIKHPSFGRNVFAISYIP---GLLFAVNGKPYFE--DQEPVQGFVMNFSSGEIIDVFKPVRKHFDMPHDIAASEDG-TVYVGDAHTNTVWKFTSTEKMEHRSVKKAGIEVQEIKESEAVVETKMENKPASSELQKIQEKQKLVKE 859          

HSP 2 Score: 220.705 bits (561), Expect = 7.770e-58
Identity = 121/297 (40.74%), Postives = 161/297 (54.21%), Query Frame = 0

Query:  402 MDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHY----KHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHT-PMTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPDNSDTPL 690
            +DI+M G  P Q+D Y C +      L      ++  +   AS D  HHM+L  C  P    G  W C   T C D++ I+YAWA++APPT+LP+ VGF + G  G  Y VLQVHY         HKD +G+S+H T       AG+YLM      IPP  ++V+ DI+C     PM +FA+R HTH LGKVV+G++V  G+     R  PQ PQ FYP++    V+ GD L ARC F    + E   IG TS+DEMCNLYIMYY +   +  F  C       I   IP  ++ P+
Sbjct:   59 LDIRMPGVTPKQSDTYFCMSV----RLPMDEEAFVIDFKPRASMDTVHHMLLFGCNMP-ASTGNYWFCDEGT-CTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPPGGKVVNSDISCHYKKYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVEHPVDVSFGDILAARCVFTGEGRTEVTHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDIFRTIPPEANIPI 349          

HSP 3 Score: 214.927 bits (546), Expect = 7.024e-56
Identity = 123/328 (37.50%), Postives = 176/328 (53.66%), Query Frame = 0

Query:   29 PSASEASAFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTPDH----TGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRA-RFLTCMDAHSTKIQENMPLDSDVPLP-PNPLLEEHAKHSSSNS 347
            P  S   A  + MPG  PK +D Y C +     LP+    F V  F+P +A  +  HHMLL+ CN P  T  G  W C   T C D+++ +YAWA+NA  T +P  V F V     ++Y VLQVHY +     D+    +G+ L+         AG++L++     IPP    V+ DI+C      P+H+FAYR H H LG V+SGY        +T I R +PQ PQAFYP++ P  +  G+ LAARC +   G  + T IG TS DEMCNLY+MYY+E   A  F+TC    +  I   +P ++++P+P  + ++  H  H  + +
Sbjct:   51 PIDSSDFALDIRMPGVTPKQSDTYFCMS---VRLPMDEEAF-VIDFKP-RASMDTVHHMLLFGCNMPAST--GNYWFCDEGT-CTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNHKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPPGGKVVNSDISCHYKK-YPMHVFAYRVHTHHLGKVVSGY--RVRNGQWTLIGRQSPQLPQAFYPVEHPVDVSFGDILAARCVFTGEGRTEVTHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDIFRTIPPEANIPIPVKSDMVMMHGHHKETEN 367          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. SwissProt
Match: gi|23503036|sp|P19021.2|AMD_HUMAN (RecName: Full=Peptidyl-glycine alpha-amidating monooxygenase; Short=PAM; Includes: RecName: Full=Peptidylglycine alpha-hydroxylating monooxygenase; Short=PHM; Includes: RecName: Full=Peptidyl-alpha-hydroxyglycine alpha-amidating lyase; AltName: Full=Peptidylamidoglycolate lyase; Short=PAL; Flags: Precursor)

HSP 1 Score: 256.529 bits (654), Expect = 1.561e-69
Identity = 153/457 (33.48%), Postives = 238/457 (52.08%), Query Frame = 0

Query:  759 GQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDAS-GNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLR----SFPAVPDDPASSGSNRLNPATKLAKESLKRFENIIPTTGVGATVAILSLVLTCL------LSLCLTCRRCRKRG---------KGAMNGGTKKKGDGKLDLGDFL----GRSKDGFQPLKQDDDEDE 1191
            GQ+SGVA+D   N ++FHRGD +W+ N+F S   Y      PIE +TIL  D +   ++ S G NLF+LPH L++DK  N WVTDVA+HQVFK  P     + P+++LG+   PG+D  H+C+PT VAV       YV+DGYCNSRI+++          +   +TQWG+ +     +   P  F +PH L L      LCVADRENGRIQCF  D+ +FV+ +K    G  V+ + Y P   G ++AV G   FG    +  +G+V++ ++G+++  F    + F+ PHD+  S DG TVY  +     VWK T  + +   +++        + +  A   +   N  T    + ++  + +I   G G  V +++ +L         +++ +  ++ R  G          G + G  + KG G L+LG+F     G S+ GF  L  +  + E
Sbjct:  512 GQVSGVALDPKNNLVIFHRGDHVWDGNSFDSKFVYQQIGLGPIEEDTILVIDPNNAAVLQSSGKNLFYLPHGLSIDKDGNYWVTDVALHQVFKLDP--NNKEGPVLILGRSMQPGSDQNHFCQPTDVAVDPGTGAIYVSDGYCNSRIVQFS--------PSGKFITQWGEESSGSSPL---PGQFTVPHSLALVPLLGQLCVADRENGRIQCFKTDTKEFVREIKHSSFGRNVFAISYIP---GLLFAVNGKPHFG--DQEPVQGFVMNFSNGEIIDIFKPVRKHFDMPHDIVASEDG-TVYIGDAHTNTVWKFTLTEKLEHRSVKKAGIEVQEIKEAEAVVETKMENKPTSSELQKMQEKQKLIKEPGSGVPVVLITTLLVIPVVVLLAIAIFIRWKKSRAFGDSEHKLETSSGRVLGRFRGKGSGGLNLGNFFASRKGYSRKGFDRLSTEGSDQE 949          

HSP 2 Score: 214.927 bits (546), Expect = 5.863e-56
Identity = 118/297 (39.73%), Postives = 160/297 (53.87%), Query Frame = 0

Query:  402 MDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHY----KHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHT-PMTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPDNSDTPL 690
            +DI+M G  P Q+D Y C +  I          ++  +   AS D  HHM+L  C  P    G  W C   T C D++ I+YAWA++APPT+LP+ VGF + G  G  Y VLQVHY         +KD +G+S+H T       AG+YLM      IP  +++V+ DI+C     PM +FA+R HTH LGKVV+G++V  G+     R  PQ PQ FYP+     V+ GD L ARC F    + E   IG TS+DEMCNLYIMYY +   +  F  C       +   IP  ++ P+
Sbjct:   59 LDIRMPGVTPKQSDTYFCMSMRIP----VDEEAFVIDFKPRASMDTVHHMLLFGCNMP-SSTGSYWFCDEGT-CTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNNKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPAGEKVVNSDISCHYKNYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSFGDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPPEANIPI 349          

HSP 3 Score: 211.846 bits (538), Expect = 5.655e-55
Identity = 122/326 (37.42%), Postives = 173/326 (53.07%), Query Frame = 0

Query:   29 PSASEASAFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTPDH----TGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRA-RFLTCMDAHSTKIQENMPLDSDVPLPPNP---LLEEHAKHS 343
            P  S   A  + MPG  PK +D Y C +  +   PV    F V  F+P +A  +  HHMLL+ CN P  T  G  W C   T C D+++ +YAWA+NA  T +P  V F V     ++Y VLQVHY +     D+    +G+ L+         AG++L++     IP     V+ DI+C      P+H+FAYR H H LG V+SGY        +T I R +PQ PQAFYP+  P  +  G+ LAARC +   G  + T IG TS DEMCNLY+MYY+E   A  F+TC    +  +   +P ++++P+P      ++ EH K +
Sbjct:   51 PIDSSDFALDIRMPGVTPKQSDTYFCMSMRI---PVDEEAF-VIDFKP-RASMDTVHHMLLFGCNMPSST--GSYWFCDEGT-CTDKANILYAWARNAPPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNNKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIPAGEKVVNSDISCHYKN-YPMHVFAYRVHTHHLGKVVSGY--RVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSFGDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPPEANIPIPVKSDMVMMHEHHKET 365          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. SwissProt
Match: gi|74872208|sp|Q9W1L5.2|PAL2_DROME (RecName: Full=Peptidyl-alpha-hydroxyglycine alpha-amidating lyase 2; AltName: Full=Peptidylamidoglycolate lyase 2; Short=dPAL2; Flags: Precursor)

HSP 1 Score: 222.246 bits (565), Expect = 2.062e-62
Identity = 128/341 (37.54%), Postives = 188/341 (55.13%), Query Frame = 0

Query:  746 VPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDA-SGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSP-----YAFNIPHGLTLAADKNLLCVADRENGRIQCFNA------DSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVEL----QPFKVWK 1070
            V NWP ++     GQ++ VA+D  G+P+VFHR +R W+ NTF  +N Y   +  PI+ NTI   DA +G I + WG+N+F++PH LT+D   N W+TDVAMHQ FKF P+     KPL+ +G+ F PG+  +H CKPT++AV+  GE F++ADGYCNSRI+K+                     N AG  +   P      +  +PH +TL    +LLC+ADREN R+ C  A        G+   +++ P++G  V+G+      G  ++AV GP     +     +G+ ID  S  ++    G    F  PH +AVS +GS +Y  E+    Q  +VWK
Sbjct:  102 VENWPTEQ--HSFGQVTAVAVDPQGSPVVFHRAERYWDVNTFNESNIYYLIEYGPIKENTIYVLDAKTGAIKSGWGSNMFYMPHGLTIDLHGNYWITDVAMHQAFKFKPF---SNKPLLTIGKRFRPGSSVKHLCKPTSIAVATTGE-FFIADGYCNSRILKF---------------------NAAGKLLRTIPQPPEFLSLQVPHAITLLEHLDLLCIADRENMRVVCPKAGLISSHGEGEPAATIQEPDLGR-VFGVASF---GDIVFAVNGPTSMLPV-----RGFTIDPRSETII----GHWGEFKNPHSMAVSVNGSALYVTEIGTNHQTNRVWK 402          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. SwissProt
Match: gi|74961531|sp|P91268.2|PGAL_CAEEL (RecName: Full=Probable peptidyl-alpha-hydroxyglycine alpha-amidating lyase pgal-1; AltName: Full=Probable peptidylamidoglycolate lyase; Short=PAL; Flags: Precursor)

HSP 1 Score: 179.489 bits (454), Expect = 2.523e-48
Identity = 114/327 (34.86%), Postives = 175/327 (53.52%), Query Frame = 0

Query:  750 PPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPF-GAITGKV----TKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKL 1071
            P KE    IGQ+SG+A++  G+ + FHR  R+W+  +F  +  +N D    I   TI        +I+ +GA LF++PH LT+D   + WVTDV  HQV K        +K ++ LG+   PG D  H+CKPT VAV+ +G  F VADGYCNSRI+K+  K +              Q N A  +    P  F +PH L+L  D N++CVADREN R+QCF+A   +  ++L P  I  T      +    GR++A+   E +   +TG       + + ID+ +G+   TF    +G    H +A++ADG  ++  +L+P ++ ++
Sbjct:   55 PSKE----IGQVSGLAVNKNGHIVAFHRSGRVWDEKSFNDHETFNKDLG-VINNKTIAIISREKKVIDEFGAGLFYMPHGLTIDNNGDYWVTDVGSHQVHKI---DAKTQKIVMSLGEKMVPGEDQAHFCKPTDVAVAKNGHIF-VADGYCNSRILKFDAKGN-----------LMAQINAA--TEENQPSEFVVPHSLSLIEDMNIVCVADRENQRVQCFSAGLSEGDRTL-PTGIPIT------SATDIGRVFAIREREHYLIGVTGNSEDVEAQMFSIDMQTGK-TETF---AKGVRNTHALAIAADG-VMFVSQLEPSRILEI 347          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. nr
Match: gi|1325271077|ref|XP_023320144.1| (peptidyl-glycine alpha-amidating monooxygenase B-like [Eurytemora affinis])

HSP 1 Score: 1067.37 bits (2759), Expect = 0.000e+0
Identity = 565/1220 (46.31%), Postives = 769/1220 (63.03%), Query Frame = 0

Query:    9 MVLCLAFTPAQPHALMGRADPSASEAS---AFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAVD--RARYLVLQVHYVNPLTTPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIE-GPRARFLTCMDAHSTK-IQENMPLDSDVPLPPNPLLEEHAKHSSSNSPHTGTHMESTSIRKGDLDNPLSAKGVDQELSVGG-RRKGDLEAPEMNQALERVKTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDGYLVLQVHYKHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHT--PMTLFAFRTHTHTLGKVVTGFKVNEGK--IHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQDESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTSGNSLEITSFIPSKIKKQDTFNRMGKDIDQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPE--PFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLRSFPAVPDDPASSGSNRLNPATKLAKESLKRFENIIPTTGVGATV-AILSLVLTCLLSLCLTCRRCRKRGKGAMNGGTKKKGDGKLDLGDFLGRSKDGFQPLKQDDDEDETGNLSSESD-DLEEFSVPALRA 1212
            ++ CL         L G  +PS++  S      + MPGA+P+  D+YLC+AF V ++       YVT F P +A A RAHHMLLYAC++P Q +PG ++DC HH++C+  SS M+AWAKNA  T +P DV F ++  + +YLVLQVHY +PL   D+TGL L YQ    +Y AGI LL    +SIP NT   H D NC++   VPLHLFA+RTHAH LGSVISGY Y+ E + Y EIA+GNPQWPQAFYPM+   T++PGE +  RCTYN+ G ++ T IG+T+GDEMCNLY+M++ E G    FL C    + + I   +P DSDV  P N + E+ AK SS N          T I + +     +     +EL  GG  +KG +E   +      V  ++  M GA P+ +D+YLC +  +K L   K +++I  + A  S ++AHHMIL KC+ P K +GEI++C HH MC DQSKI++AWAK A PT++P +VGFSLD  D  LVLQVHY  PL   D++GL++ +T   P   AGI+L+ RS L IP   E  HGD NC+V    P+ +FA+R H H+ G V++G+  NE +      A+G+P+ PQ FYP+K  + +NPG+ L ARCS+N+   ++ V IG+T  DEMCNLY+M+Y+  ++ DF+LC +E+  ++S ++PD +D PL  +   E ++   +        G SL  + F                        VPNWP   T   +GQ+SGV IDIYGN ++FHRGDR WN NTF  +N Y  ++  PI  +T++T +++G+ INSWG +LFFLPHM+TVD +NNIW+TDVA+HQ+FKFAPYGG D KPL+ LG  F PG+DD HYCKPTAVAVS D +TF+V+DGYCNSRIIKY + V  DG H V+ +T+WG+  G   SIT+ PY FN+PHGL LA D+  LCVADRENGR+QCFN +SG+F +S++PPE GS ++ + YA    G+++AV GP+  PF    G+   GY+I++ +G++  T+     G   PHDVAVS+DG T+Y VEL PFKVWKL +G+  A       P    + A +GS +L      A +       ++P       V A LS+ LT L+  CL  R+CRKRGKG M    K+K  G   LGD LGRSKDGFQP+   + E+  G +  +SD ++EEFSVPAL A
Sbjct:    4 LLFCLV-------GLAGAGEPSSAHNSLDNTIDLIMPGARPEVDDSYLCSAFEVTEMTGIQDSLYVTGFIP-KADASRAHHMLLYACSEPLQ-EPGTVYDCMHHSLCKGASSIMFAWAKNAPPTTLPKDVGFMINPIQKKYLVLQVHYAHPLPDSDNTGLSLTYQTTPARYQAGILLLGSGAVSIPGNTEVTHADGNCKLPAQVPLHLFAFRTHAHALGSVISGYAYNEEKNEYREIAKGNPQWPQAFYPMQAVQTVKPGETVTVRCTYNSTGRDRETHIGSTAGDEMCNLYIMFFTEPGKSGDFLACWGEQAGQFITRGLPADSDVAPPKNKVWEDKAKDSSVN----------TGISEINYSQLFA-----EELEKGGSNKKGGVEENTL-----MVADLNFTMPGATPSGDDEYLCTSVKVKDL-DPKNKLWIVKFSAIVSGNRAHHMILSKCEHPPKMEGEIYDCRHHQMCLDQSKILFAWAKHADPTEVPVDVGFSLDKEDS-LVLQVHYSKPLSDPDYSGLTLRFTSTEPKYTAGIFLLGRSNLNIPADTETTHGDANCKVEAKNPLHIFAYRAHAHSYGSVISGYMYNEAQRTWSLVAKGNPRWPQAFYPVKE-QIINPGEVLAARCSYNTTGHHQNVKIGSTGDDEMCNLYLMFYSLSKTDDFKLCFDEQNPRLSRMLPDGNDVPLPPNPALEEVALGRD--------GKSLPGSDF----------------------SVVPNWPSTATATSLGQISGVTIDIYGNAIIFHRGDRTWNGNTFNYDNTYAMERDLPIAFDTVVTVNSTGHTINSWGKDLFFLPHMITVDSENNIWMTDVALHQIFKFAPYGG-DHKPLLTLGTRFQPGSDDSHYCKPTAVAVSKDAKTFFVSDGYCNSRIIKYSITVTGDGKHAVNKITEWGKGAGP-FSITKGPYNFNVPHGLALAEDRAELCVADRENGRVQCFNTESGEFTRSIQPPEFGSRIFSVAYA---NGKLHAVSGPDFSPF----GQKPMGYIINMETGKLDGTWQ-VTDGLQNPHDVAVSSDGGTIYVVELSPFKVWKLMNGQPSAAAPTPVTPFSTPNLAPAGSPQLPSFMTGASK-------LMPDVSYSVIVAAALSVPLTILIMCCLAIRKCRKRGKGTMMETNKQKAWG---LGDILGRSKDGFQPV---NTEERDGLVGDDSDSEVEEFSVPALHA 1138          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. nr
Match: gi|5353835|gb|AAD42259.1|AF109920_1 (alpha-amidating enzyme precursor 2 [Lymnaea stagnalis])

HSP 1 Score: 628.246 bits (1619), Expect = 0.000e+0
Identity = 414/1188 (34.85%), Postives = 593/1188 (49.92%), Query Frame = 0

Query:   39 VNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSST-MYAWAKNAEATVMPPDVSFAVDR---ARYLVLQVHYVNPLTT---PDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRAR-FLTCMDAHSTKIQ-ENMPLDSDVPLPPNPLLEEHAKHSSSNSPHTGTHMESTSIRKGDLDNPLSAKGVDQELSVGGRRKGDLEAPEMNQALERVKTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDD-QSKIMYAWAKDAPPTQLPQNVGFSLDGND--GYLVLQVHYKHPL---EHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTPMTL--FAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQDESTD-FQLCGNEEVRKISSLIPD--NSDTPLDESQPEEPLSYANNEDEREPTSGNSLEITSFIPSKIKKQDTFNRMGKDIDQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLRSFPAVPDDPASSGSNRLNPATKLAKESLKRFENIIPTTGVGATVAILSLVLTCLLSLCLTC-----------RRCRKRGKGAMNGGTKKKGDGK----LDLGDFLGRSKDGFQPLKQDDDEDE 1191
            + M GA+P   DAYLC ++  ++       +++ +FE   AQA  AHHMLLY C+  P +   Q+W+C    +C+D+ +T ++AWAKNA  TV+P DV   +      + LVLQVHY    T    PD++G+ L+      K+ AGI+ L+    +IPP       D++C+      +  FAYRTHAH LG VI+GY ++    +Y EI +GNPQWPQAFYP+K    ++PG+ LAARCTY++        +G+T  DEMCN Y+M+Y +      +  C   H   +   N P D  VPLPPNP LEE AK         GTH                  G D      G   G +++             +I M GAKP   D YLC A+P+          YI  ++A A+A  AHH++L  C+     +  IWNC    MC   +  I++AWAK+APPT LP++VG  +        LVLQVHY       E  D +G+ ++ T  +    AG+Y M  S   IP        D++C      ++  FA+RTH H LG+V+TG++ N G  HE  +G+PQ PQ FYP+K    V PG+YL ARC+++S +   +V +G+T  DEMCN YIM+YT     D +  C       + +L P+    + P D S P  P    N E E E    +              Q      G   D  L     WP ++   ++GQ+ GVA D  GN  +FHRG R W   +F  +N +    S PI    I+  D++G ++  +GA  +F+PH + VD Q N+W+TDVA+HQVFK       + KP + LG  F    +   +CKPT VAV  +G+ F+V+DGYCNSR++K+     KDG     +L  +GQ N  G S       F+IPH +T++ + NL+CVADRENGR+QCF+ D G F   +K P+ G  ++ ++  P  GG +YAV GP   G     V +G+ +DIN+G ++  +N   +    PHDV V     +VY  EL P  VWKL+          R  PA P  P           +  AK S     +II   GV +T +  S   +   +  +                    +   +G  K  G G      +L   +G S  GF PL  ++ + E
Sbjct:  697 ILMRGAKPSKPDAYLCASYVPSE-----EEYFIYKFE-AMAQASTAHHMLLYGCDGEPFSD-QQIWNCP--LMCKDQQATILFAWAKNAPPTVLPKDVGLRIGSRTSIKTLVLQVHYARSFTESEPPDYSGITLFSTHTKPKFVAGIYFLMSPMFNIPPGETSYPIDVSCKFGAEKSIVPFAYRTHAHGLGRVITGYQHNG---SYHEIGKGNPQWPQAFYPVKDLIEVKPGDALAARCTYDSTTMAHAVSVGSTGNDEMCNFYIMFYTDSSVINPYGQCAGNHLPDVTGRNFPPDVSVPLPPNPELEEVAK---------GTHHHG------------GMSGSDPHGDGTGEGSGIMDS----------NIQEILMRGAKPKTPDAYLCTAYPV-----FDEESYIYKFEALANASVAHHILLYGCEGEPYSNEAIWNCP--AMCKSTEGIILFAWAKNAPPTVLPKDVGLRIGSTTIIKTLVLQVHYAKSFSDEEAPDHSGIKIYTTQTKQPFVAGVYFM-ASMFEIPSGFPAYPVDVSCMFDKQKSIFPFAYRTHAHALGRVITGYQYN-GSYHEIGKGNPQWPQAFYPVKDKIEVKPGEYLAARCTYDSTSMTSSVKVGSTGNDEMCNFYIMFYTDSSVLDPYGKC-------LGNLAPELTGDNFPKDVSVPLPP----NPELEEEAKGQH-------------PQHHSGMKGIHFDHNLIFNSTWPEQKV--ELGQIGGVAADRDGNVYIFHRGSRTWTAQSFSYDNNFQYQDS-PIPEEVIVILDSAGRLVRKFGAGQYFMPHGIEVDNQGNLWLTDVALHQVFKIP---AGETKPTLTLGHRFQHSENLTCFCKPTDVAVVSNGD-FFVSDGYCNSRVLKFS----KDG----QLLKAFGQRN-LGFSPAPPVGVFDIPHSITVSEENNLVCVADRENGRVQCFDLD-GNFKHMIKHPQFGPRLFAIEQCPEHGGILYAVNGPAYDGPSDFSV-QGFTMDINTGDLLEMWNVP-EHLKNPHDVCVHPASHSVYIGELNPTAVWKLS----------RLSPAQPVRPVVIQEESTQAHSGEAKLS----SDIISNLGVNSTYSDGSSDSSSDFTPSVIIGILLVIPVILLLVITLLVRAHHSGKLKCYGHGNKPRVFNLQGIMGNSHKGFDPLSTEESDHE 1775          

HSP 2 Score: 423.705 bits (1088), Expect = 3.026e-121
Identity = 245/653 (37.52%), Postives = 356/653 (54.52%), Query Frame = 0

Query:   26 RADPSASEASAFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSST-MYAWAKNAEATVMPPDVSFAVDRA---RYLVLQVHYVNPLT---TPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRAR-FLTC-MDAHSTKIQENMPLDSDVPLPPNPLLEEHAKHSSSNSPHTGTHMESTSIRKGDLDNPLSAKGVDQELSVGGRRKGDLEAPE-MNQALERVKTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMC-DDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDGY--LVLQVHYKHPL---EHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTPMTL--FAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYT 660
             +DP A ++    + M GA+P+ +DAYLCTA+ + D       +Y+ +FE   A A  AHHMLLY C+  P ++  Q+W+C    +C  + +T ++AWAKNA  T++P  V   +  +   + LVLQVHY         PDH+G+ ++   +  K+ AGIFLL+ +  SIP   +    DI+C+      +  FAYRTHAH LG VI+GY    + + Y +I +GNPQWPQAFYP+K    ++PG++LAARCTY++   +    +GAT  DEMCN Y+M+Y +      +  C  D +     +N P D  +PLPPNP LEE AK +  +                                        ++APE     +   K   I M GAKP++ D YLC ++     + ++   +I  ++A A A  AHHM+L  C      D +IWNC    MC D Q+ I++AWAK+APPT LP++VG  +        LVLQVHY       E  D++G+++  T  +P   AGIY +      IPP +     D++C+     ++  FA+RTH H LG+V+TG++ N G  HE  +G+PQ PQ FYP+K    V PGD L ARC+++S T    V +G+T  DEMCN YIM+YT
Sbjct:  349 ESDPIA-DSQTEDILMRGAKPEKSDAYLCTAYPLTD-----QEYYIYKFE-ALANASTAHHMLLYGCDGEPASK-DQIWNCP--AMCDGKQATILFAWAKNAPPTILPKGVGLRIGSSTSIKTLVLQVHYARSFEDSEAPDHSGIMIHTTHKKQKFVAGIFLLMSTSFSIPEGNSSYPVDISCKFDQEKSIFPFAYRTHAHGLGRVITGY---QKNETYHQIGKGNPQWPQAFYPVKDVIEVKPGDYLAARCTYDSTSMSHPVSVGATGNDEMCNFYIMFYTDSSVLEPYGDCSYDEYPDLTGQNFPKDVSMPLPPNPDLEEEAKGNHHHG---------------------------GMSGSSHGSSDHVDAPEDGGNTITDSKIEHILMRGAKPSKPDAYLCASY-----VPSEEEYFIYKFEAMAQASTAHHMLLYGCDGEPFSDQQIWNCP--LMCKDQQATILFAWAKNAPPTVLPKDVGLRIGSRTSIKTLVLQVHYARSFTESEPPDYSGITLFSTHTKPKFVAGIYFLMSPMFNIPPGETSYPIDVSCKFGAEKSIVPFAYRTHAHGLGRVITGYQHN-GSYHEIGKGNPQWPQAFYPVKDLIEVKPGDALAARCTYDSTTMAHAVSVGSTGNDEMCNFYIMFYT 953          

HSP 3 Score: 417.157 bits (1071), Expect = 5.193e-119
Identity = 258/693 (37.23%), Postives = 364/693 (52.53%), Query Frame = 0

Query:   39 VNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSST-MYAWAKNAEATVMPPDVSFAVDRA-RYLVLQVHYVNPLTT---PDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRAR-FLTCM-DAHSTKIQENMPLDSDVPLPPNPLLEEHAKHSSSNSPHTGTHMESTSIRKGDLDNPLSAKGVDQELSVGGRRKGDLEAPEMNQALERVKTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCK-KPIKKDGEIWNCLHHTMCD-DQSKIMYAWAKDAPPTQLPQNVGFSLDGNDGY--LVLQVHYKHPLEHK---DFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTPMTL--FAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQDESTD-FQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTSGN 714
            + M GA+PK  DAYLCTA+ V ++       Y+ +FE   A A  AHHMLLY C+  P +    +W+C    +C+   +T ++AWAKNA  TV+P  V   +    + LVLQVHY          DH+G+K+Y   Q  +Y AGI++L+  Y SIP        D++C       +  FAYRTHAH LG VI+GY ++    ++ EI +GNPQWPQAFY  +    ++ G+ LAARCTY++        +G+T  DEMCN Y+M+Y +      +  C+ D       +N P D   PLPPNP LEE AK       H G    S+     D++                           +  +   +T DI M GAKP ++D YLC A+P+          YI  ++A A+A  AHHM+L  C  +P  KD +IWNC    MCD  Q+ I++AWAK+APPT LP+ VG  +  +     LVLQVHY    E     D +G+ +H T ++    AGI+L+  +   IP        DI+C+     ++  FA+RTH H LG+V+TG++ NE   H+  +G+PQ PQ FYP+K    V PGDYL ARC+++S + +  V +GAT  DEMCN YIM+YT     + +  C  +E   ++       + P D S P  P     N D  E   GN
Sbjct:   32 ILMKGAKPKTPDAYLCTAYHVEEIKN-----YIYKFE-AVANASTAHHMLLYGCDGEPYSS-DPIWNCP--AMCKSSQATILFAWAKNAPPTVLPEGVGLRIGTTIKTLVLQVHYARSFQEEEPADHSGIKIYITKQKPQYVAGIYILMAGYFSIPSGKKSYPVDVSCSFNEEKSIFPFAYRTHAHGLGRVITGYQFNG---SHHEIGKGNPQWPQAFYSTQNKIEVKKGDKLAARCTYDSTSMTHPVSVGSTGSDEMCNFYIMFYTDSSVVNPYGQCVGDFEPLVTGQNFPKDVSDPLPPNPDLEEEAK----GHQHHGVMTASSHNPSEDVE---------------------------SDPIADSQTEDILMRGAKPEKSDAYLCTAYPL-----TDQEYYIYKFEALANASTAHHMLLYGCDGEPASKD-QIWNCP--AMCDGKQATILFAWAKNAPPTILPKGVGLRIGSSTSIKTLVLQVHYARSFEDSEAPDHSGIMIHTTHKKQKFVAGIFLLMSTSFSIPEGNSSYPVDISCKFDQEKSIFPFAYRTHAHGLGRVITGYQKNE-TYHQIGKGNPQWPQAFYPVKDVIEVKPGDYLAARCTYDSTSMSHPVSVGATGNDEMCNFYIMFYTDSSVLEPYGDCSYDEYPDLT-----GQNFPKDVSMPLPP-----NPDLEEEAKGN 662          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. nr
Match: gi|1061486723|gb|ODN03814.1| (Peptidyl-glycine alpha-amidating monooxygenase B [Orchesella cincta])

HSP 1 Score: 566.614 bits (1459), Expect = 0.000e+0
Identity = 329/862 (38.17%), Postives = 483/862 (56.03%), Query Frame = 0

Query:  392 MNQALERVKTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCK---KPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSL--DGNDGYLVLQVHYKHPLE--HKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINC--EVHTPMTLFAFRTHTHTLGKVVTGFKV--NEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ--DESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTSGNS-----LEITSFIPSKIKKQD------TFNRM------GKDIDQGLYPVPN-WPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFD-ASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGL---------SITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVEL-QPFKVWKLTDGKTIAPTTLRSFPAVPDDPASSGSNRLNPATKLAKESLKRFENIIPTTGVGATVAILSLVLTCLLSLCLTCRRCRKRGKGAMNGGTKKKGDGKLDLGDFLGRSKDGFQPLKQDDDEDETGNLSSESDDLEEFSVPALR 1211
            + + +E   ++DI M   +P ++D YLC A  I+    A    Y+  ++ N++AD+AHHM+L  C    + ++KDG  W+C HH       +I+YAWAK+APPT LP++VGF +  + N  YLVLQVHY HPL     D +G S+H   E P   AGI LMYRS L IPP +E +HGD+NC  E ++P+  FA+R HTH LG VV+G+++  N GK     +G+PQ PQ FYP K   T+NP DY+VA+C+F++    +   IG T+ DEMCNLY+MYYT+  D       CG+EE  +I++ IP +SD PL  ++  E  +  ++       + +S     +E  S     +  ++      T +R       G+ + +  +   N WP  ++   IGQ SGV  D  GN +VFHRGDR+W+Y+TF + N Y   +  PI+ +T+L  D +SG +++ WG +LF++PH +TVD + N W+TDVA+HQVFKF+P      KP ++LG+ F PG+D+ H+CKP AVAV  +G+ F+VADGYCNSR+IK+  + +K        + +WG+A    L          I+  PYAF IPH L LA + +LLCVADRENGRIQCF A SG+F   ++  E G  ++ + Y+P  GG + AV GP   G    K  +G++I + + ++ S F     GF  PHDVA+S DG+ +Y VE+ QP+KVWK +   T +    +    V   P +  S+     +     S+   E        GA+V I++ +   LL L       R R  G       K         +F   S  GF+ L+ D+ + E  +      ++EEFS    R
Sbjct:   38 LREGVEAEHSLDIAMPDVRPTKDDHYLCTALNIE----AYGTQYVHSFEPNSNADRAHHMLLFTCDDVPEMLRKDG-WWDCGHHYQACKSMRIIYAWAKNAPPTVLPKDVGFRIGAESNIKYLVLQVHYAHPLPVGSTDRSGFSLHVNSEEPKYLAGIMLMYRSMLDIPPFQEKIHGDMNCALETNSPIHPFAYRVHTHKLGVVVSGYRLDPNNGKFETIGKGNPQWPQAFYPTKTNVTINPKDYIVAQCTFSTKNFEKPTQIGMTAGDEMCNLYLMYYTKSRDPEEHNLNCGDEENAEITAHIPPDSDVPLPRNKTLEEHAMGHHSSPSTEKTSHSKKRPGIEFNSTPKLGVSDEEPGNGDTTTHRFVVYGGGGRSLKESTFKAINSWP--DSSITIGQASGVDFDTAGNIVVFHRGDRVWDYHTFDARNNYAQKEKGPIKTDTVLIIDPSSGKLLHKWGKDLFYMPHDITVDNKGNFWLTDVALHQVFKFSP---GSFKPSMILGKRFEPGSDNSHFCKPAAVAVMSNGD-FFVADGYCNSRVIKFNSRGEK--------ILEWGRAYQPNLLDGMQSWFSRISPPPYAFQIPHALALAEEHDLLCVADRENGRIQCFKASSGEFEFKIESLEFGGRLFSVAYSPANGGTLVAVNGPN--GRFQSKTVQGFIISLATREISSVFK-PASGFGNPHDVAISKDGNDIYVVEISQPYKVWKFSQNST-SSAFGKQLDMVHTGPVAPSSSVAMAHSADESNSVNSSEPATAEDSFGASVIIMAFLTIPLLLLIGIGSLMRLRDSGCCRRKETKS------FSEFFPPSA-GFEKLRMDESDSENSDT-----EVEEFSASMYR 864          

HSP 2 Score: 290.812 bits (743), Expect = 1.771e-79
Identity = 152/327 (46.48%), Postives = 196/327 (59.94%), Query Frame = 0

Query:   36 AFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQT-QPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPL--TTPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEG--PRARFLTCMDAHSTKIQENMPLDSDVPLPPNPLLEEHAK-HSSSNSPHTGTH 353
            +  + MP  +P   D YLCTA  +     A    YV  FEPN + A+RAHHMLL+ C+D P+  +    WDC HH         +YAWAKNA  TV+P DV F +      +YLVLQVHY +PL   + D +G  L+  ++  KY AGI L+ RS L IPP   K+HGD+NC + T  P+H FAYR H H+LG V+SGY        +  I +GNPQWPQAFYP K   TI P +++ A+CT++T  F + T+IG T+GDEMCNLYLMYY +   P    L C D  + +I  ++P DSDVPLP N  LEEHA  H SS S    +H
Sbjct:   47 SLDIAMPDVRPTKDDHYLCTALNIE----AYGTQYVHSFEPN-SNADRAHHMLLFTCDDVPEMLRKDGWWDCGHHYQACKSMRIIYAWAKNAPPTVLPKDVGFRIGAESNIKYLVLQVHYAHPLPVGSTDRSGFSLHVNSEEPKYLAGIMLMYRSMLDIPPFQEKIHGDMNCALETNSPIHPFAYRVHTHKLGVVVSGYRLDPNNGKFETIGKGNPQWPQAFYPTKTNVTINPKDYIVAQCTFSTKNFEKPTQIGMTAGDEMCNLYLMYYTKSRDPEEHNLNCGDEENAEITAHIPPDSDVPLPRNKTLEEHAMGHHSSPSTEKTSH 368          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. nr
Match: gi|1228358676|ref|XP_021948164.1| (peptidyl-glycine alpha-amidating monooxygenase-like [Folsomia candida] >gi|1215278898|gb|OXA65207.1| Peptidyl-glycine alpha-amidating monooxygenase B [Folsomia candida])

HSP 1 Score: 543.502 bits (1399), Expect = 7.037e-173
Identity = 337/871 (38.69%), Postives = 483/871 (55.45%), Query Frame = 0

Query:  401 TMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKK--PIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSL--DGNDGYLVLQVHYKHPLEH--KDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTPMTL--FAFRTHTHTLGKVVTGFKVNE-GKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ--DESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTSGNSLEITSFIPSK--IKKQ---------------------DTFNRMGKDI-DQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFD-ASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANG----AGLSITRS--PYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGE-GQGFNKPHDVAVSADGSTVYEVEL-QPFKVWKLTDGKTIAPT----------TLRSF------PAVPDDPASSGSNRLNPATKLAKESLKRFENIIPTTGVGATVAILSLVLTCLLSLCLTCRRCRKRGKGAMNGGTKKKGDGKLDLGDFLGRSKDGFQPLKQDDDEDETGNLSSESDDLEEFSVPALR 1211
             +D+ M G +P Q+D YLC +  I+    +    YI  ++ N+ AD+AHHM+L  C          E W+C HH       +I+YAWAK+A PT LP++VGF +  + N   LVLQVHY HPL    KD +G  +H   E P   AGI L+YRSYL IPP  + +HGD+NC + TP  +  FA+R H H LG V++G++V++ G+  +  +G+PQ PQ FYP+ +  T+ P D+L ARC++++        IG T+ +EMCNLY+M+YT+  D    + +CG+E V +I++ +P +SD PL  ++  E  ++ ++++ +  + G SL  T+  PSK   KK+                     D +   G+ + D     VP WP  ++   +GQ +GV  D  GN +VFHRGDR W Y+TF S N Y      PI+ +T+L     +G I++ WG ++F++PH +TVDK  N W+TDVAMHQVFKF P  GA   P + LG  F PGNDD H+CKP AVAV  +G+ F+VADGYCNSRIIK+  +  K        + QWG+A      +GL   RS  PYAF IPH L LA DK+LLCVADRENGRIQCFN D G F   ++    G  ++ + Y+   GG + AV GP      + K  +G+VI +N+ Q+ S F    G  F  PHDV+VS DG+ +Y VE+ QP+KVWK +  +               T +S+      P+VP  PA    +   P++KLAK+       II       T+ +L L+    +   +T   CR+R +      TK          +F   S  GF+ LK D+ + E  +      D+EEF+V + R
Sbjct:   62 VLDVSMPGVRPTQDDHYLCTSLNIE----SYGTQYIHKFEPNSDADRAHHMLLFTCDALDNTVSGQEWWDCGHHYSACRNMRIIYAWAKNAAPTVLPKDVGFRIGPESNIKNLVLQVHYAHPLPEGTKDTSGFRVHVDAEEPKYLAGILLLYRSYLDIPPHTDKIHGDMNCPLQTPAPIHPFAYRVHAHKLGVVISGYRVDQSGQFEQIGKGNPQWPQAFYPITKNLTIYPNDFLAARCTYSTTNFENATSIGQTAGNEMCNLYLMFYTESRDPEDHYLVCGDEAVPEITAHLPADSDVPLPRNKTLEEHAHHSHQNSQSSSDGLSLSQTT-TPSKSHTKKRPGAMTEPHQTYDHAEETEDPSDDYRDGGRKLPDSDYKVVPGWP--DSSVTLGQATGVDFDTEGNVVVFHRGDRTWGYDTFDSRNYYTQRNLGPIKVDTVLIIHPTNGKILHKWGKDIFYMPHDVTVDKTGNFWLTDVAMHQVFKFEP--GA-TGPSMTLGTKFVPGNDDSHFCKPAAVAVLSNGD-FFVADGYCNSRIIKFNSQGQK--------ILQWGRAFQPSFFSGL-FNRSPPPYAFQIPHALALAEDKHLLCVADRENGRIQCFNTD-GTFEFKIESSAFGGRLFSVAYSKKDGGYLMAVNGP------SQKEVQGFVIPMNTRQIQSVFKPTGGSKFGNPHDVSVSPDGNDIYVVEISQPYKVWKFSRSRKSGKAEQSPSSGVLDTSKSYPVNAENPSVPSKPAQLMED--PPSSKLAKDESFSASVIIMAF---LTIPLLLLIAVGAVMRLMTSGWCRRRRE------TKS-------FAEFFPPSA-GFEKLKMDESDSENSDT-----DIEEFAVSSYR 881          

HSP 2 Score: 308.531 bits (789), Expect = 1.188e-85
Identity = 161/324 (49.69%), Postives = 207/324 (63.89%), Query Frame = 0

Query:   26 RADPSASEASAFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQ-MWDCRHH-TVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLT--TPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEG--PRARFLTCMDAHSTKIQENMPLDSDVPLPPNPLLEEHA 340
            R + S +  S   V+MPG +P   D YLCT+  +          Y+ +FEPN + A+RAHHMLL+ C+    T  GQ  WDC HH + CR+    +YAWAKNA  TV+P DV F +      + LVLQVHY +PL   T D +G +++  A+  KY AGI LL RSYL IPP+T K+HGD+NC + TP P+H FAYR HAH+LG VISGY    ++  + +I +GNPQWPQAFYP+ +  TI P +FLAARCTY+T  F   T IG T+G+EMCNLYLM+Y E   P   +L C D    +I  ++P DSDVPLP N  LEEHA
Sbjct:   52 RENVSPTAPSVLDVSMPGVRPTQDDHYLCTSLNIESYGTQ----YIHKFEPN-SDADRAHHMLLFTCDALDNTVSGQEWWDCGHHYSACRNMR-IIYAWAKNAAPTVLPKDVGFRIGPESNIKNLVLQVHYAHPLPEGTKDTSGFRVHVDAEEPKYLAGILLLYRSYLDIPPHTDKIHGDMNCPLQTPAPIHPFAYRVHAHKLGVVISGYRVD-QSGQFEQIGKGNPQWPQAFYPITKNLTIYPNDFLAARCTYSTTNFENATSIGQTAGNEMCNLYLMFYTESRDPEDHYLVCGDEAVPEITAHLPADSDVPLPRNKTLEEHA 368          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. nr
Match: gi|1316139868|ref|XP_023214514.1| (peptidyl-glycine alpha-amidating monooxygenase-like [Centruroides sculpturatus])

HSP 1 Score: 499.975 bits (1286), Expect = 1.129e-156
Identity = 301/829 (36.31%), Postives = 451/829 (54.40%), Query Frame = 0

Query:  402 MDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKK---PIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHYKHPLEH--KDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVH--TPMTLFAFRTHTHTLGKVVTGFKVN--EGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTS---GNSLEITSFIPSKIKKQDTF--NRMGKDIDQ-----GLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDA-SGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWK-LTDGKTIAPTTLRSFPAVPDDPASSGSNRLNPA-------TKLAKESLKRFENIIPTTGVGATVAILSLVLTCLLSLCLTCRRCRKRG----------KGAMNGGTKKKGDGKLDLGDFLGRSKDGFQPLKQDDDE 1189
            ++I M   KP ++D Y+C      K + A    YI  ++ +A A +AHHMIL  C     P     E WNC+H  +C   + IMYAWAK+APP  LP +VGF + GN    Y++LQ+HY +PL     D +G+++H T +R    AGI+L+      IPP+    H D+NC      P+  FA+R H H  G V++G+K N  +      A+G+PQ PQTFYPM +  TV+ GD L ARC++NS   NE + +G+T+ DEMCNLYIMYYT  ++ + F  C + E+ K+ + +P +SD PL  +   E  ++ N  +++   S    N+     + P  I++ + F  N+  +D D          V +WP ++   K GQ++ V ID   N ++FHRGD +WN  +F   + Y   +  PI + TI+T D+ +G++++ WG+N+F++PH LT+D  + +W+TDVAMHQ+FK+ P  G D KP +  G+ F PGNDD+H+CKPT+VA+S +G+  YVADGYCNSRI+    ++   G      L QWGQ+   G +    P  F IPH +TL  DK L+CVADRENGRIQCF   SG F   +   E G  ++ +DY+P  GG  +AVCGP  +        + +V +  S Q++++F  +   F +PHD+AV+ DG+ V+  E+ P KVWK L          L   P +     S  S ++ P        T     + K+ E+ I        +  L  +   LL +  +  R +KRG          KG + G        K ++G  L   K GF  +  ++ +
Sbjct:   40 ININMPNVKPTKHDSYICTT----KRIDADD-AYIIQFEPSADALRAHHMILFGCNSLYSPNYIYPEHWNCIHSALCKKMT-IMYAWAKNAPPITLPPDVGFHVGGNSSIHYIILQLHYANPLPEGASDNSGITLHMTRKRQKYIAGIHLLLSGSAMIPPKSPKYHVDVNCYFRGRQPIHTFAYRVHAHKYGVVISGYKYNIKDNAWTFLAKGNPQWPQTFYPMDQVYTVSEGDVLAARCTYNSTQSNEPIYMGSTANDEMCNLYIMYYTNANDGSSFARCLDIEIPKLVNELPADSDVPLSPNPALEENAHTNQWNQKTSVSKKGNNAFTDAYYRPYPIQEIENFHSNKYYQDDDDYNSEFTYKEVASWPAEDR--KFGQVTAVDIDSNNNIVIFHRGDHVWNGLSFDVEDRYLLTEKGPIASPTIVTLDSVTGHVLHQWGSNIFYMPHGLTLDG-DYVWLTDVAMHQIFKY-PLSG-DIKPALTFGERFVPGNDDKHFCKPTSVAISSNGD-IYVADGYCNSRIV----RLSSSG----KFLNQWGQSIQIG-NFPPGPGDFAIPHKITLVEDKGLVCVADRENGRIQCFTLMSGNFHFEIVHKEFGGRLFSIDYSPSKGGLFFAVCGPSIY---YKHPVQAFVFNFTSRQLLNSFKPKQGTFTQPHDIAVTHDGTEVFVSEIMPNKVWKFLKVTHKSTKKVLTDMPPLQTLSPSLSSQKITPEKSTLLLHTTNKYSTTKKKESDIDNFKTSMIIMALLAIPVLLLIIITSVIRLKKRGKLQNCSLSHLKGWLGGYRTPHPQDKFNIGTLLNPHK-GFDRVAMEESD 843          

HSP 2 Score: 280.026 bits (715), Expect = 8.708e-76
Identity = 150/345 (43.48%), Postives = 202/345 (58.55%), Query Frame = 0

Query:   26 RADPSASEAS-AFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACND--PPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPL--TTPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPR-ARFLTCMDAHSTKIQENMPLDSDVPLPPNPLLEEHAKHSSSNSPHTGTHMESTSIRK 361
            RA+P   +   A  +NMP  +P   D+Y+CT   +      A   Y+ QFEP+ A A RAHHM+L+ CN    P     + W+C H  +C+ + + MYAWAKNA    +PPDV F V       Y++LQ+HY NPL     D++G+ L+   +  KY AGI LLL     IPP + K H D+NC      P+H FAYR HAH+ G VISGY Y+ + + +T +A+GNPQWPQ FYPM Q +T+  G+ LAARCTYN+   N+   +G+T+ DEMCNLY+MYY      + F  C+D    K+   +P DSDVPL PNP LEE+A        HT    + TS+ K
Sbjct:   28 RAEPPMQKDHFAININMPNVKPTKHDSYICTTKRID-----ADDAYIIQFEPS-ADALRAHHMILFGCNSLYSPNYIYPEHWNCIHSALCK-KMTIMYAWAKNAPPITLPPDVGFHVGGNSSIHYIILQLHYANPLPEGASDNSGITLHMTRKRQKYIAGIHLLLSGSAMIPPKSPKYHVDVNCYFRGRQPIHTFAYRVHAHKYGVVISGYKYNIKDNAWTFLAKGNPQWPQTFYPMDQVYTVSEGDVLAARCTYNSTQSNEPIYMGSTANDEMCNLYIMYYTNANDGSSFARCLDIEIPKLVNELPADSDVPLSPNPALEENA--------HTNQWNQKTSVSK 357          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. nr
Match: gi|918998036|ref|XP_013394833.1| (peptidyl-glycine alpha-amidating monooxygenase isoform X2 [Lingula anatina])

HSP 1 Score: 498.049 bits (1281), Expect = 2.028e-156
Identity = 319/839 (38.02%), Postives = 452/839 (53.87%), Query Frame = 0

Query:  392 MNQALERVKTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDG--NDGYLVLQVHYKHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVH--TPMTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPD---NSDTPLDESQPEEPLSYANNEDEREPTSGNSLEITSFIPSKIKKQDTFNRMGKDIDQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKS-NNEYNGDKSKPIEANTILTFDASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITR--SPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKL---------TDGKTIAPTTLRSFPAVPDDPASSGSNRLNPATK--LAKESLKRFENIIPTTGVGATVAILSLVLTCLLSLCLTCRRCRKRGKGAMNG-GTKKKG-DGKLDLGDFLGRSKDGFQPLKQDDDEDETGNLSSESDDLEEFS 1206
            + Q  E    +D+ M G  P   D Y+C + P+          YI  ++  AS D AHHM++  C++P  ++ + W C     C  +  I++AWAK+APPT LP++VGF + G  N  ++VLQ+HYK+P +  D +G+ +HYT ER  N AGI L+  + + IPP +     DI+C+    TP+  F FRTH H+LGKV+TG++ N G  H   +G+PQ PQ FYP+K    ++  D L+ RC F+S  K+    IG T  DEMCN Y+MYYT  +    F  C         +  PD   N   P  ES    P    N   E E   G+S  + S    K+ ++         +   L  V NWP    + K+GQ++GVA D  GN  +FHR DR+W+Y+TF   NN+Y      PI  +TIL+F+ +G +I  WG NLF+LPH L++D Q+NIWVTDVAMHQVFKF      ++KP + LG+ F PGND  H+CKPT VA+   GE F+V+DGYCN RIIKY      DG    SV+  WG  +G  L+  +   P AF +PHGLTLA DK  +CVADRENGRIQCF+ D G F   +   E G  VY + Y P  GG +YA+   +       +  +G+ I  ++G++  T+  +G GF++PHDVAVS DG  VY  E+   +VWK             K +     R  P  PD         ++   K  ++KE     E  +        + +  +++  ++ + +     R RG+ A N   T   G  G  +LG+ LGR   GFQ L    + +E  +L+S+S+D  EFS
Sbjct:   30 LKQHREGTNILDVTMPGITPKVEDYYVCTSVPV-----PTGENYIVQFEPLASGDMAHHMLMFGCEQP-AENRKSWECGQ--TCRGKPNILFAWAKNAPPTNLPEDVGFKIGGSSNVRHIVLQIHYKNPTKEPDASGVRLHYTPERQKNIAGIALLLSTQISIPPHQPETISDISCQFKSSTPIYPFGFRTHAHSLGKVITGYQFN-GTYHLIGKGNPQWPQAFYPVKDKIKISNNDILLGRCVFDSTHKDTYTSIGPTHNDEMCNFYMMYYTDANGGQPFYEC-------FGNWRPDMFKNGGFPSQESLTALP---PNPALEEEAEHGHS-RVDSKADDKLDEEK--------LTPALEAVKNWPDLGNV-KLGQVAGVATDSKGNVHIFHRADRVWDYSTFDPVNNQYLQRNRGPITQDTILSFNPNGKLIKQWGKNLFYLPHGLSIDDQDNIWVTDVAMHQVFKF---NKGEEKPSLTLGKEFEPGNDRYHFCKPTDVAILPTGE-FFVSDGYCNHRIIKYA----ADG----SVIKSWGTIDGE-LAADKYPKPGAFKLPHGLTLAKDKKQVCVADRENGRIQCFDYD-GNFKSQIHLTEFGHKVYAVAYTPHFGGLMYAINADQS----NPEENEGFTIKFDTGKLQDTWKPQG-GFSRPHDVAVSPDGKCVYVAEIGSNRVWKFHSTFDVGIHKSQKKMGNGIFRQKPDKPDFTTDEDEAEVDEMKKSGISKEVESNNEGSVQGENNATPIIVGVVLIVPVVVVIVATVIMRIRGRMAQNRLYTVDTGLRGGFNLGNLLGRKHKGFQQL----NTEELDHLNSDSED--EFS 814          

HSP 2 Score: 242.662 bits (618), Expect = 2.861e-63
Identity = 129/346 (37.28%), Postives = 187/346 (54.05%), Query Frame = 0

Query:    7 CLMVLCLAFTPAQPHALMGRADPSASEASAFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRAR-FLTCMDAHSTKIQENMPL---DSDVPLPPNPLLEEHAKHSSS 345
            C+++  +  +P   +             +   V MPG  PK  D Y+CT+     +PV     Y+ QFEP  A  + AHHML++ C  P + +  + W+C     CR + + ++AWAKNA  T +P DV F +      R++VLQ+HY NP   PD +G++L+Y  +  K  AGI LLL + +SIPP+  +   DI+CQ  +  P++ F +RTHAH LG VI+GY ++     Y  I +GNPQWPQAFYP+K    I   + L  RC +++   +  T IG T  DEMCN Y+MYY +    + F  C       + +N      +S   LPPNP LEE A+H  S
Sbjct:   10 CIILGFVHSSPVNDNGFSELLKQHREGTNILDVTMPGITPKVEDYYVCTS-----VPVPTGENYIVQFEP-LASGDMAHHMLMFGCEQPAENR--KSWECGQ--TCRGKPNILFAWAKNAPPTNLPEDVGFKIGGSSNVRHIVLQIHYKNPTKEPDASGVRLHYTPERQKNIAGIALLLSTQISIPPHQPETISDISCQFKSSTPIYPFGFRTHAHSLGKVITGYQFNG---TYHLIGKGNPQWPQAFYPVKDKIKISNNDILLGRCVFDSTHKDTYTSIGPTHNDEMCNFYMMYYTDANGGQPFYECFGNWRPDMFKNGGFPSQESLTALPPNPALEEEAEHGHS 342          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. nr
Match: gi|1069317286|ref|XP_018089596.1| (PREDICTED: peptidyl-glycine alpha-amidating monooxygenase B isoform X3 [Xenopus laevis] >gi|1050396366|gb|OCT98556.1| hypothetical protein XELAEV_18010794mg [Xenopus laevis])

HSP 1 Score: 498.819 bits (1283), Expect = 3.024e-156
Identity = 302/810 (37.28%), Postives = 434/810 (53.58%), Query Frame = 0

Query:  401 TMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHY----KHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTP-MTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTSGNSLEITSFIPSKIKKQDTFNRMGKDIDQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDA-SGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKT-IAPTTLRSFPAVPDDPASSGSNRLNPATKLAKESLKRFENIIPTTGVGATVAILSLVLTCLLSLCLTCRRCRKRG----------KGAMNGGTKKKGDGKLDLGDFL----GRSKDGFQPLKQD 1186
            T+DI+M G  P ++D YLC+++     L      Y+  Y  +A+ D AHHM+L  C  P   D + W+C   T C+D+S IMYAWAK+APPT+LP+ VGF + G  G  Y VLQVHY        +HKD TG+++  T E+    AGIYL       +PP +E+V+ DI C  + P +  FA+R HTH LG+VV+GF+V  GK     R  PQ PQ FYP++    ++PGD +  RC F    +     IG T+ DEMCNLYIMYY     +T +  C      K+   IP+ ++ P+  S   + +           T   +   T+    K ++++  N+     D  L    +WP      K+GQ+SG+A+D   N ++FHRGD +W+ N+F  N  Y      PI+ +TIL  D  +  ++ S G NLFFLPH LT+D+  N WVTDVA+HQVFK    G   + PL+VLG+ F PG+D +H+C+PT VAV      F+VADGYCNSRI+++          N   + QWG+   + L     P  F IPH LT+ +D+  LCVADRENGRIQCF+A +G+FVK +K  E G  V+ + YAP  GG +YAV G   +G  T    +G++++ ++G ++ TF    + F  PHD+    DG TVY  +     VWK +  KT I  T +RS P   +        +  P+      +   F  II       T+ I+ +V+   +++ +  R+ R  G           G + G  + KG G L+LG F     G S+ GF  L  +
Sbjct:   61 TLDIRMPGVTPTESDTYLCKSY----RLPVDDEAYVVDYRPHANMDTAHHMLLFGCNVPSSTD-DYWDCSAGT-CNDKSSIMYAWAKNAPPTKLPEGVGFQVGGKSGSRYFVLQVHYGDVKAFQDKHKDCTGVTVRITPEKQPLIAGIYLSMSLNTVVPPGQEVVNSDIACLYNRPTIHPFAYRVHTHQLGQVVSGFRVRHGKWTLIGRQSPQLPQAFYPVEHPLEISPGDIIATRCLFTGKGRMSATYIGGTAKDEMCNLYIMYYMDAAHATSYMTCVQTGNPKLFENIPEIANVPIPVS--PDMMMMMMMGHGHHHTEAEAETNTALQQPKREEEEVLNQ-----DVHLEEDTDWPGVNL--KVGQVSGLALDPKNNLVIFHRGDHVWDENSFDRNFVYQQRGIGPIQESTILVVDPNTSKVLKSTGQNLFFLPHGLTIDRDGNYWVTDVALHQVFKV---GAEKETPLLVLGRAFQPGSDRKHFCQPTDVAVDPITGNFFVADGYCNSRIMQFS--------PNGMFIMQWGEETSSNLP---RPGQFRIPHSLTMISDQGQLCVADRENGRIQCFHAKTGEFVKQIKHQEFGREVFAVSYAP--GGVLYAVNGKPYYGDSTP--VQGFMLNFSNGDILDTFIPARKNFEMPHDITAGDDG-TVYVGDAHANAVWKFSPSKTEIFETHMRSRPKTNESVGQQTQEK--PSVVQESSAGVSFVLII-------TLLIIPVVVLIAIAIFIRWRKVRMYGGDIGHKSESSSGGILGKLRGKGSGGLNLGTFFATHKGYSRKGFDRLSTE 827          

HSP 2 Score: 219.935 bits (559), Expect = 1.689e-55
Identity = 129/322 (40.06%), Postives = 174/322 (54.04%), Query Frame = 0

Query:   24 MGRADPSASEASA---FTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTPDH----TGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRA-RFLTCMDAHSTKIQENMPLDSDVPLPPNP 334
            +G   P  S  S+     + MPG  P  +D YLC ++    LPV     YV  + P+ A  + AHHMLL+ CN P  T     WDC   T C D+SS MYAWAKNA  T +P  V F V     +RY VLQVHY +     D     TG+ +    +     AGI+L +     +PP    V+ DI C    P  +H FAYR H HQLG V+SG+        +T I R +PQ PQAFYP++ P  I PG+ +A RC +   G    T IG T+ DEMCNLY+MYY++   A  ++TC+   + K+ EN+P  ++VP+P +P
Sbjct:   46 LGTTRPVMSPGSSDYTLDIRMPGVTPTESDTYLCKSYR---LPVDDEA-YVVDYRPH-ANMDTAHHMLLFGCNVPSSTD--DYWDCSAGT-CNDKSSIMYAWAKNAPPTKLPEGVGFQVGGKSGSRYFVLQVHYGDVKAFQDKHKDCTGVTVRITPEKQPLIAGIYLSMSLNTVVPPGQEVVNSDIACLYNRPT-IHPFAYRVHTHQLGQVVSGF--RVRHGKWTLIGRQSPQLPQAFYPVEHPLEISPGDIIATRCLFTGKGRMSATYIGGTAKDEMCNLYIMYYMDAAHATSYMTCVQTGNPKLFENIPEIANVPIPVSP 356          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. nr
Match: gi|918998032|ref|XP_013394826.1| (peptidyl-glycine alpha-amidating monooxygenase A isoform X1 [Lingula anatina])

HSP 1 Score: 489.574 bits (1259), Expect = 9.930e-153
Identity = 294/724 (40.61%), Postives = 406/724 (56.08%), Query Frame = 0

Query:  392 MNQALERVKTMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDG--NDGYLVLQVHYKHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVH--TPMTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPD---NSDTPLDESQPEEPLSYANNEDEREPTSGNSLEITSFIPSKIKKQDTFNRMGKDIDQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKS-NNEYNGDKSKPIEANTILTFDASGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITR--SPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLRSFPAVPDDPASSGSNRLNPAT 1104
            + Q  E    +D+ M G  P   D Y+C + P+          YI  ++  AS D AHHM++  C++P  ++ + W C     C  +  I++AWAK+APPT LP++VGF + G  N  ++VLQ+HYK+P +  D +G+ +HYT ER  N AGI L+  + + IPP +     DI+C+    TP+  F FRTH H+LGKV+TG++ N G  H   +G+PQ PQ FYP+K    ++  D L+ RC F+S  K+    IG T  DEMCN Y+MYYT  +    F  C         +  PD   N   P  ES    P    N   E E   G+S  + S    K+ ++         +   L  V NWP    + K+GQ++GVA D  GN  +FHR DR+W+Y+TF   NN+Y      PI  +TIL+F+ +G +I  WG NLF+LPH L++D Q+NIWVTDVAMHQVFKF      ++KP + LG+ F PGND  H+CKPT VA+   GE F+V+DGYCN RIIKY      DG    SV+  WG  +G  L+  +   P AF +PHGLTLA DK  +CVADRENGRIQCF+ D G F   +   E G  VY + Y P  GG +YA+   +       +  +G+ I  ++G++  T+  +G GF++PHDVAVS DG  VY  E+   +VWK     T    T  +  AV     +SG    NP+T
Sbjct:   30 LKQHREGTNILDVTMPGITPKVEDYYVCTSVPV-----PTGENYIVQFEPLASGDMAHHMLMFGCEQP-AENRKSWECGQ--TCRGKPNILFAWAKNAPPTNLPEDVGFKIGGSSNVRHIVLQIHYKNPTKEPDASGVRLHYTPERQKNIAGIALLLSTQISIPPHQPETISDISCQFKSSTPIYPFGFRTHAHSLGKVITGYQFN-GTYHLIGKGNPQWPQAFYPVKDKIKISNNDILLGRCVFDSTHKDTYTSIGPTHNDEMCNFYMMYYTDANGGQPFYEC-------FGNWRPDMFKNGGFPSQESLTALP---PNPALEEEAEHGHS-RVDSKADDKLDEEK--------LTPALEAVKNWPDLGNV-KLGQVAGVATDSKGNVHIFHRADRVWDYSTFDPVNNQYLQRNRGPITQDTILSFNPNGKLIKQWGKNLFYLPHGLSIDDQDNIWVTDVAMHQVFKF---NKGEEKPSLTLGKEFEPGNDRYHFCKPTDVAILPTGE-FFVSDGYCNHRIIKYA----ADG----SVIKSWGTIDGE-LAADKYPKPGAFKLPHGLTLAKDKKQVCVADRENGRIQCFDYD-GNFKSQIHLTEFGHKVYAVAYTPHFGGLMYAINADQS----NPEENEGFTIKFDTGKLQDTWKPQG-GFSRPHDVAVSPDGKCVYVAEIGSNRVWKFH--STFDAQTQSTGSAVITMKPTSGMISNNPST 703          

HSP 2 Score: 242.662 bits (618), Expect = 4.310e-63
Identity = 129/346 (37.28%), Postives = 187/346 (54.05%), Query Frame = 0

Query:    7 CLMVLCLAFTPAQPHALMGRADPSASEASAFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTPDHTGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRAR-FLTCMDAHSTKIQENMPL---DSDVPLPPNPLLEEHAKHSSS 345
            C+++  +  +P   +             +   V MPG  PK  D Y+CT+     +PV     Y+ QFEP  A  + AHHML++ C  P + +  + W+C     CR + + ++AWAKNA  T +P DV F +      R++VLQ+HY NP   PD +G++L+Y  +  K  AGI LLL + +SIPP+  +   DI+CQ  +  P++ F +RTHAH LG VI+GY ++     Y  I +GNPQWPQAFYP+K    I   + L  RC +++   +  T IG T  DEMCN Y+MYY +    + F  C       + +N      +S   LPPNP LEE A+H  S
Sbjct:   10 CIILGFVHSSPVNDNGFSELLKQHREGTNILDVTMPGITPKVEDYYVCTS-----VPVPTGENYIVQFEP-LASGDMAHHMLMFGCEQPAENR--KSWECGQ--TCRGKPNILFAWAKNAPPTNLPEDVGFKIGGSSNVRHIVLQIHYKNPTKEPDASGVRLHYTPERQKNIAGIALLLSTQISIPPHQPETISDISCQFKSSTPIYPFGFRTHAHSLGKVITGYQFNG---TYHLIGKGNPQWPQAFYPVKDKIKISNNDILLGRCVFDSTHKDTYTSIGPTHNDEMCNFYMMYYTDANGGQPFYECFGNWRPDMFKNGGFPSQESLTALPPNPALEEEAEHGHS 342          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. nr
Match: gi|148224182|ref|NP_001081254.1| (peptidyl-glycine alpha-amidating monooxygenase B precursor [Xenopus laevis] >gi|113696|sp|P12890.1|AMDB_XENLA RecName: Full=Peptidyl-glycine alpha-amidating monooxygenase B; Short=PAM-B; AltName: Full=Peptide C-terminal alpha-amidating enzyme II; Short=AE-II; AltName: Full=Peptidyl-glycine alpha-amidating monooxygenase II; Includes: RecName: Full=Peptidylglycine alpha-hydroxylating monooxygenase B; Short=PHM-B; Includes: RecName: Full=Peptidyl-alpha-hydroxyglycine alpha-amidating lyase B; AltName: Full=Peptidylamidoglycolate lyase-B; Short=PAL-B; Flags: Precursor >gi|214015|gb|AAA49667.1| alpha-amidating enzyme precursor [Xenopus laevis])

HSP 1 Score: 489.189 bits (1258), Expect = 2.339e-152
Identity = 305/843 (36.18%), Postives = 448/843 (53.14%), Query Frame = 0

Query:  401 TMDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHY----KHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHTP-MTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTSGNSLEITSFIPSKIKKQDTFNRMGKDIDQGLYPVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDA-SGNIINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCGPEPFGAITGKVTKGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLRSFPAVPDDPASSGSN---RLNPAT-KLAKESLKRFENIIPTTGVGA------TVAILSLVLTCLLSLCLTCRRCRKRG----------KGAMNGGTKKKGDGKLDLGDFL----GRSKDGFQPLKQDDDEDETGNLSSESDDLEEFSVPAL 1210
            T+DI+M G  P ++D YLC+++     L      Y+  Y  +A+ D AHHM+L  C  P   D + W+C   T C+D+S IMYAWAK+APPT+LP+ VGF + G  G  Y VLQVHY        +HKD TG+++  T E+    AGIYL       +PP +E+V+ DI C  + P +  FA+R HTH LG+VV+GF+V  GK     R  PQ PQ FYP++    ++PGD +  RC F    +     IG T+ DEMCNLYIMYY     +T +  C      K+   IP+ ++ P+  S   + +           T   +   T+    K ++++  N+     D  L    +WP      K+GQ+SG+A+D   N ++FHRGD +W+ N+F  N  Y      PI+ +TIL  D  +  ++ S G NLFFLPH LT+D+  N WVTDVA+HQVFK    G   + PL+VLG+ F PG+D +H+C+PT VAV      F+VADGYCNSRI+++          N   + QWG+   + L     P  F IPH LT+ +D+  LCVADRENGRIQCF+A +G+FVK +K  E G  V+ + YAP  GG +YAV G   +G  T    +G++++ ++G ++ TF    + F  PHD+A   DG TVY  +     VWK +  K    +  ++   V +   +       R  P T +   +  +   +++  +  G       T+ I+ +V+   +++ +  R+ R  G           G + G  + KG G L+LG F     G S+ GF  L  +  + E  +    SD  EE+S P +
Sbjct:   61 TLDIRMPGVTPTESDTYLCKSY----RLPVDDEAYVVDYRPHANMDTAHHMLLFGCNVPSSTD-DYWDCSAGT-CNDKSSIMYAWAKNAPPTKLPEGVGFQVGGKSGSRYFVLQVHYGDVKAFQDKHKDCTGVTVRITPEKQPLIAGIYLSMSLNTVVPPGQEVVNSDIACLYNRPTIHPFAYRVHTHQLGQVVSGFRVRHGKWTLIGRQSPQLPQAFYPVEHPLEISPGDIIATRCLFTGKGRMSATYIGGTAKDEMCNLYIMYYMDAAHATSYMTCVQTGNPKLFENIPEIANVPIPVS--PDMMMMMMMGHGHHHTEAEAETNTALQQPKREEEEVLNQ-----DVHLEEDTDWPGVNL--KVGQVSGLALDPKNNLVIFHRGDHVWDENSFDRNFVYQQRGIGPIQESTILVVDPNTSKVLKSTGQNLFFLPHGLTIDRDGNYWVTDVALHQVFKV---GAEKETPLLVLGRAFQPGSDRKHFCQPTDVAVDPITGNFFVADGYCNSRIMQFS--------PNGMFIMQWGEETSSNLP---RPGQFRIPHSLTMISDQGQLCVADRENGRIQCFHAKTGEFVKQIKHQEFGREVFAVSYAP--GGVLYAVNGKPYYGDSTP--VQGFMLNFSNGDILDTFIPARKNFEMPHDIAAGDDG-TVYVGDAHANAVWKFSPSKAEHRSVKKAGIEVEEITETEIFETHMRSRPKTNESVGQQTQEKPSVVQESSAGVSFVLIITLLIIPVVVLIAIAIFIRWRKVRMYGGDIGHKSESSSGGILGKLRGKGSGGLNLGTFFATHKGYSRKGFDRLSTEGSDQEKDDDDDGSDSEEEYSAPPI 869          

HSP 2 Score: 219.935 bits (559), Expect = 1.969e-55
Identity = 129/322 (40.06%), Postives = 174/322 (54.04%), Query Frame = 0

Query:   24 MGRADPSASEASA---FTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTPDH----TGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRA-RFLTCMDAHSTKIQENMPLDSDVPLPPNP 334
            +G   P  S  S+     + MPG  P  +D YLC ++    LPV     YV  + P+ A  + AHHMLL+ CN P  T     WDC   T C D+SS MYAWAKNA  T +P  V F V     +RY VLQVHY +     D     TG+ +    +     AGI+L +     +PP    V+ DI C    P  +H FAYR H HQLG V+SG+        +T I R +PQ PQAFYP++ P  I PG+ +A RC +   G    T IG T+ DEMCNLY+MYY++   A  ++TC+   + K+ EN+P  ++VP+P +P
Sbjct:   46 LGTTRPVMSPGSSDYTLDIRMPGVTPTESDTYLCKSYR---LPVDDEA-YVVDYRPH-ANMDTAHHMLLFGCNVPSSTD--DYWDCSAGT-CNDKSSIMYAWAKNAPPTKLPEGVGFQVGGKSGSRYFVLQVHYGDVKAFQDKHKDCTGVTVRITPEKQPLIAGIYLSMSLNTVVPPGQEVVNSDIACLYNRPT-IHPFAYRVHTHQLGQVVSGF--RVRHGKWTLIGRQSPQLPQAFYPVEHPLEISPGDIIATRCLFTGKGRMSATYIGGTAKDEMCNLYIMYYMDAAHATSYMTCVQTGNPKLFENIPEIANVPIPVSP 356          

BLAST of af109920_1alpha-amidating enzyme precursor 2 vs. nr
Match: gi|975127492|ref|XP_015279578.1| (PREDICTED: peptidyl-glycine alpha-amidating monooxygenase isoform X3 [Gekko japonicus])

HSP 1 Score: 488.034 bits (1255), Expect = 5.114e-152
Identity = 298/835 (35.69%), Postives = 447/835 (53.53%), Query Frame = 0

Query:  402 MDIQMNGAKPNQNDDYLCQAFPIKKLLKAKTRVYITGYDANASADKAHHMILQKCKKPIKKDGEIWNCLHHTMCDDQSKIMYAWAKDAPPTQLPQNVGFSLDGNDG--YLVLQVHY----KHPLEHKDFTGLSMHYTDERPTNFAGIYLMYRSYLRIPPQKEIVHGDINCEVHT-PMTLFAFRTHTHTLGKVVTGFKVNEGKIHEFARGDPQKPQTFYPMKRFETVNPGDYLVARCSFNSMTKNETVDIGATSADEMCNLYIMYYTQ-DESTDFQLCGNEEVRKISSLIPDNSDTPLDESQPEEPLSYANNEDEREPTSGNSLEITSFIPSKIKKQDTFNRMGKDIDQGLY--PVPNWPPKETMDKIGQLSGVAIDIYGNPMVFHRGDRIWNYNTFKSNNEYNGDKSKPIEANTILTFDASGN-IINSWGANLFFLPHMLTVDKQNNIWVTDVAMHQVFKFAPYGGADKKPLIVLGQPFSPGNDDRHYCKPTAVAVSDDGETFYVADGYCNSRIIKYQLKVDKDGYHNVSVLTQWGQANGAGLSITRSPYAFNIPHGLTLAADKNLLCVADRENGRIQCFNADSGKFVKSLKPPEIGSTVYGLDYAPVGGGRIYAVCG-PEPFGAITGKVT--KGYVIDINSGQVVSTFNGEGQGFNKPHDVAVSADGSTVYEVELQPFKVWKLTDGKTIAPTTLR----SFPAVPDDPASSGSNRLNP--ATKLAKESLKRFENIIPTTGVGA------TVAILSLVLTCLLSLCLTCRRCRKRG----------KGAMNGGTKKKGDGKLDLGDFL----GRSKDGFQPLK-----QDDDEDE 1191
            +DI+M G  P Q+D Y C +      L  +   Y+  +  +AS D  HHM+L  C +P   D + W+C   T C D S I+YAWA++APPT+LP+ VGF + G  G  Y VLQVHY        +HKD +G+++H T+++    AG+YLM      IPP +++V  DI C     PM LFA+R HTH LG+VV+G++V +G+     R  PQ PQ FYP++    V  GD L ARC F    +     IG+TS+DEMCN YIMYY +   +  F  C      ++   IP  ++ P+        +++ +NE+ R           S +  + K++D      + +DQ L    V +WP      K+GQ+SG+A+D   N +VFHRGD +W+ N+F +   Y      PIE +TIL  D S + +++S G  +F+LPH L++DK +N WVTDVA+HQVFK A +G   + PL+ LG+ F PG+D  H+C+PT  AV       YV+DGYCNSRII++          N   +TQWG+      +    P  FNIPH LT+ +D   LCVADRENGRIQCF  ++G+FVK ++ P  G  ++ + YAP  GG ++AV G P P     G V   + ++++ ++G+++ TF    + F  PHD+  + D  TVY  +     VWK T  + +   +++        + +  A   +   N   +T+L K+  K    +I  +  G       T+ I+ +V+   +++ +  R+ R  G           G + G  + KG G L+LG+F     G S+ GF  L      Q+ DED+
Sbjct:   60 LDIRMPGVTPTQSDTYFCMSLR----LPVEEEAYVVDFKPHASMDTVHHMLLFGCNEP-TSDEDYWDCDAGT-CTDTSNILYAWARNAPPTRLPKGVGFKVGGEKGSKYFVLQVHYGDISAFKDKHKDCSGVTLHLTNQKQPMIAGMYLMMSMDTIIPPGEKVVDADIACHYKMYPMHLFAYRVHTHRLGQVVSGYRVRDGQWTLIGRQSPQLPQAFYPVENPVEVKYGDTLAARCVFTGEGRASKTRIGSTSSDEMCNFYIMYYIEAKHAVSFMTCTQNVNPEMFKSIPQEANIPIPVKSDMTMMAHGHNEEARRGQ-------LSSVAQQPKREDE-----EVLDQKLSIEEVADWPGINL--KVGQVSGLALDPNSNLVVFHRGDHVWDGNSFDNRYIYQQKGLGPIEESTILVLDPSNSAVLHSTGKKMFYLPHGLSIDKNSNYWVTDVALHQVFKLASHG--VESPLLTLGRAFQPGSDRNHFCQPTDAAVDPVTGNVYVSDGYCNSRIIQFS--------PNGIFITQWGEETSLAQA---KPGQFNIPHSLTMVSDFGQLCVADRENGRIQCFKPETGEFVKEIRHPLFGKELFAVSYAP--GGLLFAVNGKPHP-----GDVEPLQAFMMNFSTGEIIDTFIPLRKNFEMPHDIVATED-RTVYIGDAHANTVWKFTSTEKMEHRSVKKAGIEVQEIKEAEAVIEARLTNKPVSTELLKKEEK--HRVIQQSSAGVSFVLITTLLIIPIVVLLAIAVFIRWRKTRVYGADAEHKLESSSGRILGRFRGKGSGGLNLGNFFASRAGYSRKGFDRLSTEGSDQEKDEDD 851          

HSP 2 Score: 216.853 bits (551), Expect = 1.688e-54
Identity = 121/304 (39.80%), Postives = 169/304 (55.59%), Query Frame = 0

Query:   36 AFTVNMPGAQPKGTDAYLCTAFAVADLPVAAHPFYVTQFEPNQAQAERAHHMLLYACNDPPQTQPGQMWDCRHHTVCRDRSSTMYAWAKNAEATVMPPDVSFAV---DRARYLVLQVHYVNPLTTPDH----TGLKLYYQAQVTKYSAGIFLLLRSYLSIPPNTAKVHGDINCQVATPVPLHLFAYRTHAHQLGSVISGYIYSAETDNYTEIARGNPQWPQAFYPMKQPHTIQPGEFLAARCTYNTLGFNQTTKIGATSGDEMCNLYLMYYIEGPRA-RFLTCMDAHSTKIQENMPLDSDVPLP 331
            A  + MPG  P  +D Y C +     LPV     YV  F+P+ A  +  HHMLL+ CN+P  T     WDC   T C D S+ +YAWA+NA  T +P  V F V     ++Y VLQVHY +     D     +G+ L+   Q     AG++L++     IPP    V  DI C      P+HLFAYR H H+LG V+SGY        +T I R +PQ PQAFYP++ P  ++ G+ LAARC +   G    T+IG+TS DEMCN Y+MYYIE   A  F+TC    + ++ +++P ++++P+P
Sbjct:   59 ALDIRMPGVTPTQSDTYFCMSLR---LPVEEEA-YVVDFKPH-ASMDTVHHMLLFGCNEP--TSDEDYWDCDAGT-CTDTSNILYAWARNAPPTRLPKGVGFKVGGEKGSKYFVLQVHYGDISAFKDKHKDCSGVTLHLTNQKQPMIAGMYLMMSMDTIIPPGEKVVDADIACHYKM-YPMHLFAYRVHTHRLGQVVSGY--RVRDGQWTLIGRQSPQLPQAFYPVENPVEVKYGDTLAARCVFTGEGRASKTRIGSTSSDEMCNFYIMYYIEAKHAVSFMTCTQNVNPEMFKSIPQEANIPIP 351