BLAST of Calsequestrin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000003928 (supercontig:LSalAtl2s:LSalAtl2s211:1365220:1367124:-1 gene:EMLSAG00000003928 transcript:EMLSAT00000003928 description:"maker-LSalAtl2s211-augustus-gene-13.18")

HSP 1 Score: 264.618 bits (675), Expect = 4.361e-84
Identity = 127/195 (65.13%), Postives = 157/195 (80.51%), Query Frame = 0

Query:    4 EIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDG-EPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGRAV 197
            E EKDEGIF+L  KNYD+A+K ++Y+LVEF+APWCGHCKAL PEYVKAAQ L+E+DS IKL KVDGT EQEL+ K  + GYPT+ FYR+G EPI Y+GGRMA EI  W+EKK GPPA+ L +AE+ + +++D+DVAV+GF+KD +S  AKKY +AVRDYE YP A TSDE+  +  KV D  VVLFKKFDEG+AV
Sbjct:   25 EFEKDEGIFILTEKNYDEAIKNFEYILVEFYAPWCGHCKALDPEYVKAAQMLREKDSPIKLGKVDGTVEQELITKFQVSGYPTLRFYREGEEPIKYNGGRMAXEIVAWLEKKIGPPAIFLNTAEEAEFFVEDNDVAVIGFYKDKESKNAKKYISAVRDYEVYPMAFTSDEKIFEKYKVNDETVVLFKKFDEGKAV 219          

HSP 2 Score: 228.409 bits (581), Expect = 2.847e-70
Identity = 106/194 (54.64%), Postives = 153/194 (78.87%), Query Frame = 0

Query:  192 DEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGD--NEAVEYNGERTLEGITKFLASGGEYGKAAPDHDE 383
            DE    RV+++LGLK +KMPT+RIIQM + ++ K+KP+    +KDN+++F+ ++L+ K+   Y ++D+P DW+   VK L  KNFD+VAF+K K+VLVEFYAPWCGHCK+L PIW+EV E L+ KD++VVAK+D+T NE+SHT+++S+PTI+L+KK D  +E ++YNGERT EGI KFL++ G YG+AAP+  E
Sbjct:  298 DEDDNRRVIDYLGLKKDKMPTMRIIQMKD-EIWKFKPDDTTVAKDNIQKFIDEYLEGKILRDYKTEDLPSDWNAKHVKYLTAKNFDDVAFDKTKDVLVEFYAPWCGHCKKLAPIWDEVGESLADKDNVVVAKIDATLNEMSHTQIQSYPTIRLYKKDDDSDEYIDYNGERTKEGILKFLSTDGIYGQAAPEEGE 490          

HSP 3 Score: 73.9442 bits (180), Expect = 1.425e-14
Identity = 39/101 (38.61%), Postives = 63/101 (62.38%), Query Frame = 0

Query:  271 LVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMV-VAKMDSTTNE--LSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFL 368
            L  KN+DE A    + +LVEFYAPWCGHCK L P + + A+ L +KD  + + K+D T  +  ++  +V  +PT++ +++G+ E ++YNG R    I  +L
Sbjct:   35 LTEKNYDE-AIKNFEYILVEFYAPWCGHCKALDPEYVKAAQMLREKDSPIKLGKVDGTVEQELITKFQVSGYPTLRFYREGE-EPIKYNGGRMAXEIVAWL 133          

HSP 4 Score: 72.4034 bits (176), Expect = 4.160e-14
Identity = 42/103 (40.78%), Postives = 61/103 (59.22%), Query Frame = 0

Query:   14 LNAKNYDK-AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYR----DGEPIDYSGGRMANEIAGWV 111
            L AKN+D  A  + K +LVEF+APWCGHCK LAP + +  + L ++D+ + +AK+D T  +  M    I+ YPTI  Y+      E IDY+G R    I  ++
Sbjct:  376 LTAKNFDDVAFDKTKDVLVEFYAPWCGHCKKLAPIWDEVGESLADKDN-VVVAKIDATLNE--MSHTQIQSYPTIRLYKKDDDSDEYIDYNGERTKEGILKFL 475          

BLAST of Calsequestrin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000012191 (supercontig:LSalAtl2s:LSalAtl2s880:2638:4218:-1 gene:EMLSAG00000012191 transcript:EMLSAT00000012191 description:"maker-LSalAtl2s880-augustus-gene-0.6")

HSP 1 Score: 244.588 bits (623), Expect = 7.543e-77
Identity = 117/196 (59.69%), Postives = 148/196 (75.51%), Query Frame = 0

Query:  192 DEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGEYG---KAAPDHDEL 384
            DE    R++EF G+K +++PT+R+I++ E D+ K++P+  E ++ N+  F+K F D  +  H LS+++P+DWDK  VK LVGKNF+EVA NKDKNVLVEFYAPWCGHCKQLVPIWEE+ +  + K+D+V+AKMDSTTNEL   KV  FPTIKLFKKG NE V YNGERTLEG TKFL S G  G   K+  DHDEL
Sbjct:  294 DEEDHKRILEFFGVKEDELPTMRLIKLEE-DMSKFRPDNLEITESNIRAFIKSFFDGTLKQHLLSEEVPKDWDKEDVKVLVGKNFEEVAMNKDKNVLVEFYAPWCGHCKQLVPIWEELGKNFADKEDIVIAKMDSTTNELESIKVTGFPTIKLFKKGSNEVVNYNGERTLEGFTKFLESDGVDGAEAKSGGDHDEL 488          

HSP 2 Score: 204.912 bits (520), Expect = 1.471e-61
Identity = 97/200 (48.50%), Postives = 138/200 (69.00%), Query Frame = 0

Query:    4 EIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVE-DGQVVLFKKFDEGRAVRVVEF 202
            EI +DEG+ VL  KN+D A++++ ++LVEF+APWCGHCKALAPE+ KAA  L +++S I L KVD TEE EL EK  ++GYPT+  +R+G+P++ +GGR A+ I  W+EKK GPPA  L++ E+VKD   D  VAV+G FKD +SD AK Y  A    ++    I+S +    + +++ D  V+L KKFDEGR  +  +F
Sbjct:   21 EITQDEGVLVLTTKNFDSAIEKHDFMLVEFYAPWCGHCKALAPEFAKAAGALADKESSIMLGKVDATEEGELAEKFGVRGYPTLKLFRNGKPVEXNGGRTADTIIAWLEKKXGPPAATLKTVEEVKDATKDVKVAVLGLFKDVESDAAKAYLDAALSMDDETFLISSQDAVFTEYEIKGDSAVILLKKFDEGRNDKTDDF 220          

HSP 3 Score: 80.8777 bits (198), Expect = 7.690e-17
Identity = 54/134 (40.30%), Postives = 76/134 (56.72%), Query Frame = 0

Query:  238 LEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLS-KKDDMVVAKMDST-TNELSHT-KVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFL 368
            + +F+  FL       Y  D+I +D     V  L  KNFD  A  K   +LVEFYAPWCGHCK L P + + A  L+ K+  +++ K+D+T   EL+    VR +PT+KLF+ G  + VE NG RT + I  +L
Sbjct:    1 MNRFLGVFLVCLAATCYGEDEITQD---EGVLVLTTKNFDS-AIEKHDFMLVEFYAPWCGHCKALAPEFAKAAGALADKESSIMLGKVDATEEGELAEKFGVRGYPTLKLFRNG--KPVEXNGGRTADTIIAWL 128          

HSP 4 Score: 69.707 bits (169), Expect = 3.344e-13
Identity = 40/118 (33.90%), Postives = 72/118 (61.02%), Query Frame = 0

Query:    1 MFQEIEKD---EGIFVLNAKNYDK-AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDG--EPIDYSGGRMANEIAGWVE 112
            + +E+ KD   E + VL  KN+++ A+ + K +LVEF+APWCGHCK L P + +  +   +++ +I +AK+D T  +  +E + + G+PTI  ++ G  E ++Y+G R       ++E
Sbjct:  356 LSEEVPKDWDKEDVKVLVGKNFEEVAMNKDKNVLVEFYAPWCGHCKQLVPIWEELGKNFADKE-DIVIAKMDSTTNE--LESIKVTGFPTIKLFKKGSNEVVNYNGERTLEGFTKFLE 470          

BLAST of Calsequestrin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000002084 (supercontig:LSalAtl2s:LSalAtl2s138:50916:54410:1 gene:EMLSAG00000002084 transcript:EMLSAT00000002084 description:"maker-LSalAtl2s138-augustus-gene-0.6")

HSP 1 Score: 131.339 bits (329), Expect = 4.734e-34
Identity = 62/169 (36.69%), Postives = 100/169 (59.17%), Query Frame = 0

Query:    8 DEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIK 176
            D  + VL  KN+D+ +K++  L+V+F+APWCGHCK ++P+Y K A +LK+  S+  + K+D T      +K  + GYPT+ F+  G PIDY G R  N+I  ++EKK+G      +   ++   ++ + +A++GFFKD +S  A  +  A R        ITSD+  +K
Sbjct:   33 DXDVIVLTDKNFDEMIKKHDMLMVKFYAPWCGHCKEMSPDYEKLATKLKKSKSKFAIGKLDATVSMTTGQKYRVTGYPTLKFFFKGLPIDYEGARENNKILKFMEKKSGDSTKTFKDETELNKMVERNSLAIIGFFKDLNSKEANIFIEAARLTSYVEFLITSDKSLMK 201          

HSP 2 Score: 99.3673 bits (246), Expect = 4.619e-23
Identity = 58/187 (31.02%), Postives = 101/187 (54.01%), Query Frame = 0

Query:  199 VVEFLGLKNEKMPTLRIIQMTEMDVVKYKP--ETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRS--FPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGEYGKAAPDH 381
            +  + G   EK P   I  +   DV K+ P  +    +  NL +F+  +L+KK    +LS ++P+DWD  P+K +V KNF E+  +K K+ ++ + +  C  C   + + +E+A+     DD V+A ++   NE     + +  FPTI++FKK  NE VE+ G+ +++ + KFL    EY    P++
Sbjct:  323 MFHYFGGDIEKTPFFIIFNLA--DVSKFHPHEDANTLTTKNLVKFIDLYLNKKAKQFFLSGELPDDWDSKPLKYIVQKNFMEITHDKKKDAMILYISNECEQCGDKMTMMKELAQEFKDSDDKVLAYINGGINEFKDESLTTDLFPTIRIFKKKTNEMVEFLGQFSVDNLRKFLI---EYDVQKPEY 504          

HSP 3 Score: 76.2554 bits (186), Expect = 2.399e-15
Identity = 47/121 (38.84%), Postives = 67/121 (55.37%), Query Frame = 0

Query:  268 VKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSK-KDDMVVAKMDSTTNELSHTKVR--SFPTIKLFKKGDNEAVEYNGERTLEGITKFL-ASGGEYGKAAPDHDEL 384
            V  L  KNFDE+   K   ++V+FYAPWCGHCK++ P +E++A  L K K    + K+D+T +  +  K R   +PT+K F KG    ++Y G R    I KF+    G+  K   D  EL
Sbjct:   36 VIVLTDKNFDEM-IKKHDMLMVKFYAPWCGHCKEMSPDYEKLATKLKKSKSKFAIGKLDATVSMTTGQKYRVTGYPTLKFFFKG--LPIDYEGARENNKILKFMEKKSGDSTKTFKDETEL 153          

BLAST of Calsequestrin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000011331 (supercontig:LSalAtl2s:LSalAtl2s781:193816:195340:-1 gene:EMLSAG00000011331 transcript:EMLSAT00000011331 description:"maker-LSalAtl2s781-augustus-gene-2.32")

HSP 1 Score: 107.842 bits (268), Expect = 4.697e-26
Identity = 61/142 (42.96%), Postives = 93/142 (65.49%), Query Frame = 0

Query:  230 TEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFN-KDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNEL-SHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLA 369
            +E FS D+LE+FV D+   K+  +  S+DIP++  KN VK  V KNF E+  + K+K++L+EFYAPWCGHCK+L PI+EE+ E + K +++++ K+D+T N++ S   VR FPT+     G  + V Y G R      K++A
Sbjct:  323 SEAFSVDSLEKFVSDYEAGKLEPYMKSEDIPDN-SKNVVKIAVAKNFKELITDEKEKDILIEFYAPWCGHCKKLTPIYEELGESM-KDENVLITKIDATANDVPSEFNVRGFPTLFWIPAG-GKPVNYEGGREKLDFIKYIA 461          

HSP 2 Score: 92.4337 bits (228), Expect = 1.054e-20
Identity = 49/140 (35.00%), Postives = 82/140 (58.57%), Query Frame = 0

Query:    9 EGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEE-QELMEKMHIKGYPTIFFYRDGE-PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYI-DDSDVAVVGFFKD 145
            E +  L   ++D +++ Y   LV F+APWCGHCK L PE+ K+  +L E D  ++L KVD TE  ++   +  ++GYPT+  +R GE   +Y+G R A  I   ++ + GP +   +S  +VK+Y+    +V +V + +D
Sbjct:   18 ENVVDLGDSDFDSSMEGYDTALVMFYAPWCGHCKKLKPEFEKSGGDLLENDPPVQLVKVDCTEAGKDTCARFEVRGYPTLKIFRAGELSSEYNGPRDAAGITKHMKSQVGPASKTFKSVVEVKEYLKSKKEVVIVSYGQD 157          

HSP 3 Score: 70.0922 bits (170), Expect = 1.927e-13
Identity = 33/90 (36.67%), Postives = 58/90 (64.44%), Query Frame = 0

Query:   16 AKNYDKAVKEYKY--LLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFY-RDGEPIDYSGGR 102
            AKN+ + + + K   +L+EF+APWCGHCK L P Y +  + +K  D  + + K+D T   ++  + +++G+PT+F+    G+P++Y GGR
Sbjct:  365 AKNFKELITDEKEKDILIEFYAPWCGHCKKLTPIYEELGESMK--DENVLITKIDAT-ANDVPSEFNVRGFPTLFWIPAGGKPVNYEGGR 451          

HSP 4 Score: 68.9366 bits (167), Expect = 5.179e-13
Identity = 38/89 (42.70%), Postives = 54/89 (60.67%), Query Frame = 0

Query:  286 NVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMV-VAKMDST---TNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLAS 370
              LV FYAPWCGHCK+L P +E+    L + D  V + K+D T    +  +  +VR +PT+K+F+ G+  + EYNG R   GITK + S
Sbjct:   37 TALVMFYAPWCGHCKKLKPEFEKSGGDLLENDPPVQLVKVDCTEAGKDTCARFEVRGYPTLKIFRAGELSS-EYNGPRDAAGITKHMKS 124          

BLAST of Calsequestrin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000001034 (supercontig:LSalAtl2s:LSalAtl2s1171:182736:187083:1 gene:EMLSAG00000001034 transcript:EMLSAT00000001034 description:"augustus_masked-LSalAtl2s1171-processed-gene-1.7")

HSP 1 Score: 99.7525 bits (247), Expect = 3.789e-23
Identity = 59/153 (38.56%), Postives = 83/153 (54.25%), Query Frame = 0

Query:   11 IFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADS---DLAKKYKAAVRD 160
            + VL   N+ K +K  K  L  F+APWC HCK + PE   AA ELKE    IK+ KVDGT E+EL ++  I G+PT   +R G   +Y G R  + I  +++ +  PP+    S   V + +D  +  VVGFFK + S   DL  +Y  A  +
Sbjct:   48 VVVLTTDNFTKYIKNEKLTLAMFYAPWCKHCKQVEPEIEGAAAELKEWG--IKIVKVDGTREKELADQYGIIGWPTFKMFRKGRIYNYDGPREKDNIIAFMKNQYKPPSEEKSSRLGVVNNMDRLEATVVGFFKGSASEHTDLFDEYIVAANE 198          

HSP 2 Score: 70.8626 bits (172), Expect = 1.465e-13
Identity = 45/165 (27.27%), Postives = 87/165 (52.73%), Query Frame = 0

Query:  232 EFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVV-AKMDSTTNELSHT--KVRSFPTIKLFKKGDN-EAVEYNGERTLEGI--------TKFLASGGEYGKAAPDHDEL 384
            +F  + L  F+      +V  +  S  +P+  ++  VK LV  N+D+      K+ ++ F+APWCGHCK L P++++VA+ +S  ++ V+  KM+  +N++ +   K++ FP++      D  + + Y G++  + I        + FL      G+AA + DE+
Sbjct:  361 DFESEYLSNFINLLRSGRVSPYMKSLPVPQG-EQGLVKNLVANNYDDEIHKIKKDAVIFFHAPWCGHCKDLDPVFKKVAKKISTNNENVIFGKMNGESNDIPYMFPKLKGFPSLFFISAYDKFDPILYQGDKNYKSIRDWINRHSSIFLTEEERTGEAALEDDEI 524          

HSP 3 Score: 67.3958 bits (163), Expect = 2.019e-12
Identity = 39/121 (32.23%), Postives = 60/121 (49.59%), Query Frame = 0

Query:   14 LNAKNYDKAVKEYKY-LLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFF---YRDGEPIDYSGGRMANEIAGWV----------EKKTGPPAL 120
            L A NYD  + + K   ++ FHAPWCGHCK L P + K A+++   +  +   K++G           +KG+P++FF   Y   +PI Y G +    I  W+          E++TG  AL
Sbjct:  399 LVANNYDDEIHKIKKDAVIFFHAPWCGHCKDLDPVFKKVAKKISTNNENVIFGKMNGESNDIPYMFPKLKGFPSLFFISAYDKFDPILYQGDKNYKSIRDWINRHSSIFLTEEERTGEAAL 519          

HSP 4 Score: 51.9878 bits (123), Expect = 1.600e-7
Identity = 38/103 (36.89%), Postives = 53/103 (51.46%), Query Frame = 0

Query:  268 VKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTT-NELS-HTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFL 368
            V  L   NF +   N +K  L  FYAPWC HCKQ+ P  E  A  L K+  + + K+D T   EL+    +  +PT K+F+KG      Y+G R  + I  F+
Sbjct:   48 VVVLTTDNFTKYIKN-EKLTLAMFYAPWCKHCKQVEPEIEGAAAEL-KEWGIKIVKVDGTREKELADQYGIIGWPTFKMFRKG--RIYNYDGPREKDNIIAFM 146          

BLAST of Calsequestrin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000000034 (supercontig:LSalAtl2s:LSalAtl2s1006:23836:28203:-1 gene:EMLSAG00000000034 transcript:EMLSAT00000000034 description:"maker-LSalAtl2s1006-augustus-gene-0.23")

HSP 1 Score: 88.9669 bits (219), Expect = 2.598e-19
Identity = 46/143 (32.17%), Postives = 84/143 (58.74%), Query Frame = 0

Query:    3 QEIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPID-YSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFK 144
            +E++ + G+ VL+  N+   +    +  ++F+APWCGHC+ L+P + + A E K+ ++ +K+AK+D T+ Q++ ++  + GYPT+ FYRDG+ ++ Y G R  +++  +V+  T  P        K KD I + D A     K
Sbjct:  888 EEVKVENGLHVLSEDNFKAFIGSGNHF-IKFYAPWCGHCQKLSPIWDELASEFKD-NANVKIAKMDCTQAQDICQEQDVTGYPTLSFYRDGQKVESYRGXRAISDLKDFVQTMTDVP--------KPKDTIVEGDSAPSSIIK 1020          

HSP 2 Score: 79.7221 bits (195), Expect = 2.523e-16
Identity = 43/110 (39.09%), Postives = 64/110 (58.18%), Query Frame = 0

Query:   22 AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTG--PPALVLESAEKVK 129
            ++KE   + V+F APWC HCK LAP +   A+E  ++D  +KLA+VD T E E    M + GYPT+ F+   E + Y G R  + +  ++E + G   P   + SAE+VK
Sbjct:  785 SLKESGAIFVKFFAPWCSHCKRLAPTWETLAKEYSDKD--VKLARVDCTIETEFCSDMDVTGYPTLKFF-GKEEVKYRGQRDEDSLVKFIETQMGRIKPEETIVSAEEVK 891          

HSP 3 Score: 73.559 bits (179), Expect = 2.353e-14
Identity = 50/142 (35.21%), Postives = 76/142 (53.52%), Query Frame = 0

Query:  242 VKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNV-LVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTN-----ELSHTK-VRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGEYGK 376
            +KDF+     V    D I E  D  P  +++  N +E   N    V  ++FYAPWCGHCK+L PIWEE+A      D + +AK+D T N     EL + + V  FPT+ ++  G+ +  EY G R +E + +F+    ++ K
Sbjct:  991 LKDFVQTMTDVPKPKDTIVEG-DSAP-SSIIKINKEEFKENIASGVTFIKFYAPWCGHCKRLAPIWEELAVKFEGVDYVKIAKVDCTANDNKNKELCNEEGVNGFPTLNIYASGE-KVKEYRGNRKVEDLEEFVKEHSKFLK 1129          

HSP 4 Score: 70.4774 bits (171), Expect = 2.944e-13
Identity = 48/162 (29.63%), Postives = 80/162 (49.38%), Query Frame = 0

Query:  211 PTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNE--LSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLAS 370
            PTL+     E   VKY+ + +E   D+L +F++  + +  P   +     E   +N +  L   NF   AF    N  ++FYAPWCGHC++L PIW+E+A       ++ +AKMD T  +       V  +PT+  ++ G  +   Y G R +  +  F+ +
Sbjct:  846 PTLKFFGKEE---VKYRGQRDE---DSLVKFIETQMGRIKPEETIVS-AEEVKVENGLHVLSEDNFK--AFIGSGNHFIKFYAPWCGHCQKLSPIWDELASEFKDNANVKIAKMDCTQAQDICQEQDVTGYPTLSFYRDG-QKVESYRGXRAISDLKDFVQT 997          

HSP 5 Score: 70.0922 bits (170), Expect = 3.306e-13
Identity = 37/104 (35.58%), Postives = 61/104 (58.65%), Query Frame = 0

Query:  279 VAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDST--TNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGEYGKAAPD 380
            ++  +   + V+F+APWC HCK+L P WE +A+  S K D+ +A++D T  T   S   V  +PT+K F K   E V+Y G+R  + + KF+ +  + G+  P+
Sbjct:  784 ISLKESGAIFVKFFAPWCSHCKRLAPTWETLAKEYSDK-DVKLARVDCTIETEFCSDMDVTGYPTLKFFGK---EEVKYRGQRDEDSLVKFIET--QMGRIKPE 881          

HSP 6 Score: 61.6178 bits (148), Expect = 1.831e-10
Identity = 32/91 (35.16%), Postives = 55/91 (60.44%), Query Frame = 0

Query:   30 LVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGT----EEQELMEKMHIKGYPTIFFYRDGEPI-DYSGGRMANEIAGWVEKKT 115
             ++F+APWCGHCK LAP + + A + +  D  +K+AKVD T    + +EL  +  + G+PT+  Y  GE + +Y G R   ++  +V++ +
Sbjct: 1036 FIKFYAPWCGHCKRLAPIWEELAVKFEGVDY-VKIAKVDCTANDNKNKELCNEEGVNGFPTLNIYASGEKVKEYRGNRKVEDLEEFVKEHS 1125          

BLAST of Calsequestrin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000008577 (supercontig:LSalAtl2s:LSalAtl2s529:99352:100677:-1 gene:EMLSAG00000008577 transcript:EMLSAT00000008577 description:"augustus_masked-LSalAtl2s529-processed-gene-1.3")

HSP 1 Score: 78.1814 bits (191), Expect = 4.484e-16
Identity = 62/230 (26.96%), Postives = 101/230 (43.91%), Query Frame = 0

Query:   30 LVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFY-----RDGEPIDYSGGRMANEIAGWVEKK-----TGPPALVLESAEKVKDYIDDSDVAVVGFFK---DADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGRAVRVVE-FLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDF 245
            L  F APWCGHCK L P +  AA ELK +   +KL  VD T    L ++  ++GYPTI ++     RDG P ++ GGR   +I  W  ++       P  L +    ++K++ +   + V+ F     D  S+    Y   ++            E   K  K+  G +     + E  A   +E  + +     P + ++   +M   KY   T  F KD + +F++D 
Sbjct:  184 LXXFFAPWCGHCKNLEPHWKSAASELKGK---VKLGAVDATVYPGLAQQYGVQGYPTIKYFPSGLKRDG-PEEFDGGRTKEDIVAWALERFELNLPAPDVLQIVGESQIKEHCESKPLCVISFLPHILDCQSECRNSYIKILK------------ETGNKFKKLGWGWL-----WAEATAQSDLESAMDVGGFGYPAMTVLSAKKM---KYSMLTGSFGKDGIHEFLRDL 389          

HSP 2 Score: 75.0998 bits (183), Expect = 5.251e-15
Identity = 39/102 (38.24%), Postives = 51/102 (50.00%), Query Frame = 0

Query:  265 KNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITK 366
            K+ V  L   NFD    + D   LVEFYAPWCGHC++LVP +E+  + L     +     D      S   V  FPTIK+F         YNG+RT +G  +
Sbjct:   27 KSGVVDLNKGNFDSRVTDSDGVALVEFYAPWCGHCQKLVPEYEKAGKALKGLITVGAVNCDEEKALCSQFGVNGFPTIKVFADNKKSPEAYNGDRTAQGFVR 128          

HSP 3 Score: 73.1738 bits (178), Expect = 2.086e-14
Identity = 41/103 (39.81%), Postives = 57/103 (55.34%), Query Frame = 0

Query:    6 EKDEGIFVLNAKNYDKAVKEYKYL-LVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE--PIDYSGGRMAN 105
             K  G+  LN  N+D  V +   + LVEF+APWCGHC+ L PEY KA + LK     I +  V+  EE+ L  +  + G+PTI  + D +  P  Y+G R A 
Sbjct:   25 SKKSGVVDLNKGNFDSRVTDSDGVALVEFYAPWCGHCQKLVPEYEKAGKALK---GLITVGAVNCDEEKALCSQFGVNGFPTIKVFADNKKSPEAYNGDRTAQ 124          

HSP 4 Score: 60.4622 bits (145), Expect = 2.968e-10
Identity = 38/115 (33.04%), Postives = 49/115 (42.61%), Query Frame = 0

Query:  268 VKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKG--DNEAVEYNGERTLEGITKFLASGGEYGKAAPD 380
            V  L   NF +   + D   L  F+APWCGHCK L P W+  A  L  K  +                V+ +PTIK F  G   +   E++G RT E I  +     E    APD
Sbjct:  164 VVELTDSNFKKEVLDSDDMWLXXFFAPWCGHCKNLEPHWKSAASELKGKVKLGAVDATVYPGLAQQYGVQGYPTIKYFPSGLKRDGPEEFDGGRTKEDIVAWALERFELNLPAPD 278          

BLAST of Calsequestrin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000003447 (supercontig:LSalAtl2s:LSalAtl2s188:668906:672178:1 gene:EMLSAG00000003447 transcript:EMLSAT00000003447 description:"maker-LSalAtl2s188-augustus-gene-7.23")

HSP 1 Score: 77.411 bits (189), Expect = 1.161e-15
Identity = 85/359 (23.68%), Postives = 154/359 (42.90%), Query Frame = 0

Query:   29 LLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPID---YSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDV--AVVGFFKD---ADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKM--DSTTNELSHTKVRSFPTIKLFKKGDNEAVE--YNGERTLEGITKFLASGGEYG 375
            + V+F +P C  C    PE+ KA+  +      IK   VD T+   +  K +I+ YPT  ++ +G   D   + G     EIA +V     P  + L+     K     S +   V+ F+ +       +A ++    R + E P  + +        KV+  +    + F + +AVR            P++RI          Y    + F    L +    F D  V  H ++  + ++ D + ++ L+ K++ +        V+V+FYAPWCG C++L P +EE A  L  K  ++  K+  D   +      ++++PT+ L++ G  E +E  +N E  +  I K +    + G
Sbjct:  489 IFVDFFSPHCSPCLNFLPEFRKASVLI---GGSIKFVSVDCTKNPTICNKFNIRAYPTGMYF-NGSITDKHLFHGSHRDQEIADFVMDIVRPKVVTLDERVFKKLIYGQSPIKTIVIDFYANWCGPCMQMAPEWNKLGRLFSENPTVMIA--------KVDCEE---HRDFCDRQAVR----------SYPSMRI----------YTDPNKYFIFTGLHREAFAFRDW-VYKHMIT--VAKELDDSSLQNLINKSYKQP-------VVVDFYAPWCGPCQRLAPKFEEAALSLQAK-GVIFGKVNCDYFRHLCGRHHIQAYPTLLLYRNGHPEDLETSFNTESIINAILKIVEDDTDSG 801          

HSP 2 Score: 63.929 bits (154), Expect = 2.568e-11
Identity = 35/97 (36.08%), Postives = 55/97 (56.70%), Query Frame = 0

Query:   24 KEYKY-LLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPID----YSGGRMANEIAGWVEKKT 115
            K YK  ++V+F+APWCG C+ LAP++ +AA  L+ +   +   KV+    + L  + HI+ YPT+  YR+G P D    ++   + N I   VE  T
Sbjct:  704 KSYKQPVVVDFYAPWCGPCQRLAPKFEEAALSLQAKG--VIFGKVNCDYFRHLCGRHHIQAYPTLLLYRNGHPEDLETSFNTESIINAILKIVEDDT 798          

HSP 3 Score: 52.373 bits (124), Expect = 1.323e-7
Identity = 21/66 (31.82%), Postives = 40/66 (60.61%), Query Frame = 0

Query:   27 KYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRD 92
            K ++++F+A WCG C  +APE+ K  +   E +  + +AKVD  E ++  ++  ++ YP++  Y D
Sbjct:  597 KTIVIDFYANWCGPCMQMAPEWNKLGRLFSE-NPTVMIAKVDCEEHRDFCDRQAVRSYPSMRIYTD 661          

BLAST of Calsequestrin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000001823 (supercontig:LSalAtl2s:LSalAtl2s1314:111717:117623:1 gene:EMLSAG00000001823 transcript:EMLSAT00000001823 description:"maker-LSalAtl2s1314-snap-gene-0.41")

HSP 1 Score: 72.0182 bits (175), Expect = 5.755e-14
Identity = 29/81 (35.80%), Postives = 51/81 (62.96%), Query Frame = 0

Query:   14 LNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE 94
            L+  ++D+ + +   + V F+APWCGHCK + P +  AA  L +   + KL  VD T E  L +++ +KGYPT+ ++++G+
Sbjct:  294 LDNGSFDEVISKETSINVMFYAPWCGHCKKMKPHFSAAANRLHDEGVDGKLGAVDCTSEDALCKRLGVKGYPTVKYFKNGK 374          

HSP 2 Score: 71.2478 bits (173), Expect = 1.170e-13
Identity = 34/105 (32.38%), Postives = 59/105 (56.19%), Query Frame = 0

Query:   14 LNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTI----FFYRDGEPIDYSGGRMANEIAGWVEKK 114
            L   ++   +K  K+++V F+APWCGHCK    E+  AA EL   +   + A +D  +E+ +    ++ GYPT     +F ++G P++Y+GGR   +   ++E K
Sbjct:  418 LTTGSFKSFLKRKKHVVVMFYAPWCGHCKKAKSEFGSAA-ELFRDNPRTEFAAIDCIDEKTICSDYNVSGYPTFKYFSYFNKNGTPLEYNGGRSRKDFIKFMEDK 521          

HSP 3 Score: 60.077 bits (144), Expect = 4.050e-10
Identity = 40/124 (32.26%), Postives = 63/124 (50.81%), Query Frame = 0

Query:  256 SDDIPEDWDKNPVKTLVGKNFDEVAFNK------DKNVLVEFYAPWCGHCKQLVPIWEEVAE-FLSKKDDMVVAKMDSTTNELSHTK----VRSFPTIKLFKKGDNEAVEYNGERTLEGITKFL 368
            + DIP  W ++P    V    D  +F K      ++ +LV FYAPWCG CK+L PI+++ AE  ++      +A MD    E +  +    + +FPTI L+ +G+    +Y GE   +    FL
Sbjct:  144 TGDIP--WHEDPHAYHVHHLTDPSSFKKFIKSSENQKILVMFYAPWCGFCKRLKPIYQKAAETVMTDIQGAAMAAMDVNRPENAPIRKKFNITAFPTI-LYFEGNEMRYQYPGENDADSFVNFL 264          

HSP 4 Score: 56.6102 bits (135), Expect = 4.967e-9
Identity = 39/117 (33.33%), Postives = 59/117 (50.43%), Query Frame = 0

Query:  252 VHYLSDDIPED-----------W---DKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKD-DMVVAKMDSTTNE--LSHTKVRSFPTIKLFKKGDNE 351
            V++L +  PED           W   D + V  L   +FDEV  +K+ ++ V FYAPWCGHCK++ P +   A  L  +  D  +  +D T+ +       V+ +PT+K FK G  E
Sbjct:  261 VNFLKNPSPEDEIIPKKAIQLGWKDEDTSTVVHLDNGSFDEV-ISKETSINVMFYAPWCGHCKKMKPHFSAAANRLHDEGVDGKLGAVDCTSEDALCKRLGVKGYPTVKYFKNGKFE 376          

HSP 5 Score: 53.5286 bits (127), Expect = 5.063e-8
Identity = 30/90 (33.33%), Postives = 43/90 (47.78%), Query Frame = 0

Query:  283 KDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNE--LSHTKVRSFPTIKLFK--KGDNEAVEYNGERTLEGITKFL 368
            + K+V+V FYAPWCGHCK+    +   AE          A +D    +   S   V  +PT K F     +   +EYNG R+ +   KF+
Sbjct:  429 RKKHVVVMFYAPWCGHCKKAKSEFGSAAELFRDNPRTEFAAIDCIDEKTICSDYNVSGYPTFKYFSYFNKNGTPLEYNGGRSRKDFIKFM 518          

HSP 6 Score: 51.2174 bits (121), Expect = 2.385e-7
Identity = 25/72 (34.72%), Postives = 40/72 (55.56%), Query Frame = 0

Query:   25 EYKYLLVEFHAPWCGHCKALAPEYVKAAQELKE--RDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE 94
            E + +LV F+APWCG CK L P Y KAA+ +    + + +    V+  E   + +K +I  +PTI ++   E
Sbjct:  175 ENQKILVMFYAPWCGFCKRLKPIYQKAAETVMTDIQGAAMAAMDVNRPENAPIRKKFNITAFPTILYFEGNE 246          

BLAST of Calsequestrin-1 precursor vs. L. salmonis genes
Match: EMLSAG00000008392 (supercontig:LSalAtl2s:LSalAtl2s512:630694:636500:-1 gene:EMLSAG00000008392 transcript:EMLSAT00000008392 description:"maker-LSalAtl2s512-snap-gene-5.21")

HSP 1 Score: 60.077 bits (144), Expect = 3.821e-10
Identity = 30/88 (34.09%), Postives = 49/88 (55.68%), Query Frame = 0

Query:  278 EVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAE-FLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKK---GDNEAVEYNGERTL 361
            E+  + DK  +VEFYA WCGHC +  P W+++A  F + KD +V+A  +    + S  ++   PTIK+F+     +N   E +  + L
Sbjct:   37 ELLSSPDKIWVVEFYAHWCGHCIRYAPFWKKIASLFQTWKDSIVIAAFNCAEYDCSTYQIPGTPTIKVFRPYNGSNNNGFEISSHKRL 124          

BLAST of Calsequestrin-1 precursor vs. SwissProt
Match: gi|30923135|sp|P09102.3|PDIA1_CHICK (RecName: Full=Protein disulfide-isomerase; Short=PDI; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=Retina cognin; Short=R-cognin; Flags: Precursor)

HSP 1 Score: 220.705 bits (561), Expect = 6.089e-66
Identity = 101/195 (51.79%), Postives = 139/195 (71.28%), Query Frame = 0

Query:  185 VVLFKKFDEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNE-AVEYNGERTLEGITKFLASGGEYGKAA 378
            + +F   D     R++EF GLK E+ P +R+I + E ++ KYKPE+++ + D +++F   FL+ K+  H +S D+PEDWDK PVK LVGKNF+EVAF+++KNV VEFYAPWCGHCKQL PIW+++ E     +++V+AKMDST NE+   K+ SFPT+K F  G     ++YNGERTLEG  KFL SGG+ G AA
Sbjct:  292 LFIFIDSDHSDNQRILEFFGLKKEECPAVRLITLEE-EMTKYKPESDDLTADKIKEFCNKFLEGKIKPHLMSQDLPEDWDKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYRDHENIVIAKMDSTANEVEAVKIHSFPTLKFFPAGSGRNVIDYNGERTLEGFKKFLESGGQDGAAA 485          

HSP 2 Score: 194.126 bits (492), Expect = 9.205e-56
Identity = 123/353 (34.84%), Postives = 195/353 (55.24%), Query Frame = 0

Query:    1 MFQEIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGRAVRVVEFLG-LKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVH---YLSDDIPEDWDK-NPVKTLVGKNFDEVAF------NKDKNVLVEFYAPWCGHCK--QLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVR 337
            + + +E+++G+ VL A N+++A+  +++LLVEF+APWCGHCKALAPEY KAA +LK   SEI+LAKVD TEE EL ++  ++GYPTI F+R+G+   P +Y+ GR A++I  W++K+TGP A  L  A   +  +D S+V V+GFFKD  SD AK++  A    ++ P  I+S        ++    VVLFKKFDEGR      F G L  + +  L  I+  ++      P   EF+    EQ        ++  H   +L   + +   K +  KT  G    ++ F      + D   ++EF+      C   +L+ + EE+ ++  + DD+   K+    N+    K++
Sbjct:   21 LAEPLEEEDGVLVLRAANFEQALAAHRHLLVEFYAPWCGHCKALAPEYAKAAAQLKAEGSEIRLAKVDATEEAELAQQFGVRGYPTIKFFRNGDKAAPREYTAGREADDIVSWLKKRTGPAATTLTDAAAAETLVDSSEVVVIGFFKDVTSDAAKEFLLAAESVDDIPFGISSSADVFSKYQLSQDGVVLFKKFDEGRN----NFEGDLTKDNL--LNFIKSNQL------PLVIEFT----EQTAPKIFGGEIKTHILLFLPKSVSDYEGKLDNFKTAAGNFKGKILFIFIDSDHSDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESDDLTADKIKEFCNKFLEGKIK 357          

HSP 3 Score: 71.633 bits (174), Expect = 2.520e-12
Identity = 43/105 (40.95%), Postives = 62/105 (59.05%), Query Frame = 0

Query:  268 VKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFL-SKKDDMVVAKMDSTTN-ELSHT-KVRSFPTIKLFKKGDNEA-VEYNGERTLEGITKFL 368
            V  L   NF++ A    +++LVEFYAPWCGHCK L P + + A  L ++  ++ +AK+D+T   EL+    VR +PTIK F+ GD  A  EY   R  + I  +L
Sbjct:   31 VLVLRAANFEQ-ALAAHRHLLVEFYAPWCGHCKALAPEYAKAAAQLKAEGSEIRLAKVDATEEAELAQQFGVRGYPTIKFFRNGDKAAPREYTAGREADDIVSWL 134          

HSP 4 Score: 66.2402 bits (160), Expect = 1.336e-10
Identity = 38/94 (40.43%), Postives = 58/94 (61.70%), Query Frame = 0

Query:   13 VLNAKNYDK-AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEP---IDYSGGR 102
            VL  KN+++ A  E K + VEF+APWCGHCK LAP + K  +  ++ ++ I +AK+D T  +  +E + I  +PT+ F+  G     IDY+G R
Sbjct:  376 VLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYRDHEN-IVIAKMDSTANE--VEAVKIHSFPTLKFFPAGSGRNVIDYNGER 466          

BLAST of Calsequestrin-1 precursor vs. SwissProt
Match: gi|33112403|sp|Q17770.1|PDI2_CAEEL (RecName: Full=Protein disulfide-isomerase 2; AltName: Full=PDI 1; AltName: Full=Prolyl 4-hydroxylase subunit beta-2; Flags: Precursor)

HSP 1 Score: 218.779 bits (556), Expect = 2.110e-65
Identity = 96/178 (53.93%), Postives = 135/178 (75.84%), Query Frame = 0

Query:  198 RVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGEYG 375
            R++EF GLK +++P +R+I + E D+ K+KP+ EE + +N+ +F +++LD  V  H +S+DIPEDWDKNPVK LVGKNF++VA +  KNVLVEFYAPWCGHCKQL P W+++ E  +  + +V+AKMDST NE+   K++SFPTIK F  G N+ V+Y G+RT+EG TKFL + G+ G
Sbjct:  296 RIMEFFGLKKDELPAIRLISLEE-DMTKFKPDFEEITTENISKFTQNYLDGSVKPHLMSEDIPEDWDKNPVKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLETNGKEG 472          

HSP 2 Score: 180.644 bits (457), Expect = 6.118e-51
Identity = 84/191 (43.98%), Postives = 129/191 (67.54%), Query Frame = 0

Query:    5 IEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGR 195
            IE++E + VL   N+D+ +   +++LVEF+APWCGHCK+LAPEY KAA +LKE  S+IKL K+D T   E+  K  ++GYPT+  +R+G+P +Y+GGR  + I  W++KKTGP A  L  A+ VK+  + +DV V+G+FKD  SD AK +       ++ P  I++++    + +++   +VLFKKFD+GR
Sbjct:   19 IEEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADVVVIGYFKDTTSDDAKTFLEVAAGIDDVPFGISTEDAVKSEIELKGEGIVLFKKFDDGR 209          

HSP 3 Score: 78.5666 bits (192), Expect = 1.056e-14
Identity = 45/104 (43.27%), Postives = 64/104 (61.54%), Query Frame = 0

Query:  268 VKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKK-DDMVVAKMDSTTNELSHTK--VRSFPTIKLFKKGDNEAVEYNGERTLEGITKFL 368
            V  L   NFDEV  N ++ +LVEFYAPWCGHCK L P + + A  L ++  D+ + K+D+T +    +K  VR +PT+KLF+ G  +  EYNG R  + I  +L
Sbjct:   25 VIVLTKDNFDEV-INGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQ--EYNGGRDHDSIIAWL 125          

HSP 4 Score: 70.4774 bits (171), Expect = 4.667e-12
Identity = 38/103 (36.89%), Postives = 62/103 (60.19%), Query Frame = 0

Query:   13 VLNAKNYDKAVKE-YKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEP--IDYSGGRMANEIAGWVE 112
            +L  KN+++  ++  K +LVEF+APWCGHCK LAP + K  ++  + D  I +AK+D T  +  +E + I+ +PTI F+  G    +DY+G R       ++E
Sbjct:  367 ILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFAD-DESIVIAKMDSTLNE--VEDVKIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLE 466          

BLAST of Calsequestrin-1 precursor vs. SwissProt
Match: gi|129730|sp|P21195.1|PDIA1_RABIT (RecName: Full=Protein disulfide-isomerase; Short=PDI; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor)

HSP 1 Score: 216.853 bits (551), Expect = 1.899e-64
Identity = 103/201 (51.24%), Postives = 140/201 (69.65%), Query Frame = 0

Query:  185 VVLFKKFDEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNEAV-EYNGERTLEGITKFLASGGEYGKAAPDHDEL 384
            + +F   D     R++EF GLK E+ P +R+I + E ++ KYKPE++E + + + +F + FL+ K+  H +S ++PEDWD+ PVK LVGKNF+EVAF++ KNV VEFYAPWCGHCKQL PIW+++ E   +  D+V+AKMDST NE+   KV SFPT+K F  G    V +YNGERTL+G  KFL SGG+ G  A D D L
Sbjct:  288 LFIFIDSDHADNQRILEFFGLKKEECPAVRLITLEE-EMTKYKPESDELTAEGITEFCQRFLEGKIKPHLMSQELPEDWDRQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKEHQDIVIAKMDSTANEVEAVKVHSFPTLKFFPAGPGRTVIDYNGERTLDGFKKFLESGGQDG--AGDEDGL 485          

HSP 2 Score: 187.963 bits (476), Expect = 1.722e-53
Identity = 88/193 (45.60%), Postives = 132/193 (68.39%), Query Frame = 0

Query:    6 EKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGR 195
            E+++ + VL + N+ + +  +K+LLVEF+APWCGHCKALAPEY KAA +LK   S+I+LAKVD TEE +L ++  ++GYPTI F+++G+   P +Y+ GR A++I  W++K+TGP A  L  +   +  ++ S+VAV+GFFKD +SD AK++  A    ++ P  +T+        +V    VVLFKKFDEGR
Sbjct:   22 EEEDNVLVLKSSNFAEELAAHKHLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSDIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLADSAAAESLVESSEVAVIGFFKDVESDAAKQFLLAAEATDDIPFGLTASSDVFSRYQVHQDGVVLFKKFDEGR 214          

HSP 3 Score: 71.2478 bits (173), Expect = 2.704e-12
Identity = 43/109 (39.45%), Postives = 62/109 (56.88%), Query Frame = 0

Query:  264 DKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFL-SKKDDMVVAKMDST--TNELSHTKVRSFPTIKLFKKGDNEAV-EYNGERTLEGITKFL 368
            +++ V  L   NF E      K++LVEFYAPWCGHCK L P + + A  L ++  D+ +AK+D+T  ++      VR +PTIK FK GD  +  EY   R  + I  +L
Sbjct:   23 EEDNVLVLKSSNFAE-ELAAHKHLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSDIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWL 130          

HSP 4 Score: 67.3958 bits (163), Expect = 4.817e-11
Identity = 44/119 (36.97%), Postives = 69/119 (57.98%), Query Frame = 0

Query:    1 MFQEIEKD---EGIFVLNAKNYDK-AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEP---IDYSGGRMANEIAGWVE 112
            M QE+ +D   + + VL  KN+++ A  E K + VEF+APWCGHCK LAP + K  +  KE   +I +AK+D T  +  +E + +  +PT+ F+  G     IDY+G R  +    ++E
Sbjct:  357 MSQELPEDWDRQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKEH-QDIVIAKMDSTANE--VEAVKVHSFPTLKFFPAGPGRTVIDYNGERTLDGFKKFLE 472          

BLAST of Calsequestrin-1 precursor vs. SwissProt
Match: gi|129731|sp|P04785.2|PDIA1_RAT (RecName: Full=Protein disulfide-isomerase; Short=PDI; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; Flags: Precursor)

HSP 1 Score: 216.468 bits (550), Expect = 2.499e-64
Identity = 103/201 (51.24%), Postives = 141/201 (70.15%), Query Frame = 0

Query:  185 VVLFKKFDEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLF-KKGDNEAVEYNGERTLEGITKFLASGGEYGKAAPDHDEL 384
            + +F   D     R++EF GLK E+ P +R+I + E ++ KYKPE++E + + + QF   FL+ K+  H +S ++PEDWDK PVK LVGKNF+EVAF++ KNV VEFYAPWCGHCKQL PIW+++ E     +++V+AKMDST NE+   KV SFPT+K F    D   ++YNGERTL+G  KFL SGG+ G  A D+D+L
Sbjct:  289 LFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEE-EMTKYKPESDELTAEKITQFCHHFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDG--AGDNDDL 486          

HSP 2 Score: 183.726 bits (465), Expect = 7.037e-52
Identity = 92/194 (47.42%), Postives = 132/194 (68.04%), Query Frame = 0

Query:    5 IEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGR 195
            +E+++ + VL   N+ +A+  + YLLVEF+APWCGHCKALAPEY KAA +LK   SEI+LAKVD TEE +L ++  ++GYPTI F+++G+   P +Y+ GR A++I  W++K+TGP A  L      +  +D S+V V+GFFKDA SD AK++  A    ++ P  ITS+       +++   VVLFKKFDEGR
Sbjct:   22 LEEEDNVLVLKKSNFAEALAAHNYLLVEFYAPWCGHCKALAPEYAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLSDTAAAESLVDSSEVTVIGFFKDAGSDSAKQFLLAAEAVDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGR 215          

HSP 3 Score: 67.3958 bits (163), Expect = 5.509e-11
Identity = 43/119 (36.13%), Postives = 70/119 (58.82%), Query Frame = 0

Query:    1 MFQEIEKD---EGIFVLNAKNYDK-AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFY---RDGEPIDYSGGRMANEIAGWVE 112
            M QE+ +D   + + VL  KN+++ A  E K + VEF+APWCGHCK LAP + K  +  K+ ++ I +AK+D T  +  +E + +  +PT+ F+    D   IDY+G R  +    ++E
Sbjct:  358 MSQELPEDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHEN-IVIAKMDSTANE--VEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLE 473          

HSP 4 Score: 63.5438 bits (153), Expect = 8.941e-10
Identity = 42/105 (40.00%), Postives = 57/105 (54.29%), Query Frame = 0

Query:  268 VKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKK-DDMVVAKMDST--TNELSHTKVRSFPTIKLFKKGDNEAV-EYNGERTLEGITKFL 368
            V  L   NF E A      +LVEFYAPWCGHCK L P + + A  L  +  ++ +AK+D+T  ++      VR +PTIK FK GD  +  EY   R  + I  +L
Sbjct:   28 VLVLKKSNFAE-ALAAHNYLLVEFYAPWCGHCKALAPEYAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWL 131          

BLAST of Calsequestrin-1 precursor vs. SwissProt
Match: gi|1709616|sp|P54399.1|PDI_DROME (RecName: Full=Protein disulfide-isomerase; Short=PDI; Short=dPDI; Flags: Precursor)

HSP 1 Score: 215.698 bits (548), Expect = 3.159e-64
Identity = 103/204 (50.49%), Postives = 141/204 (69.12%), Query Frame = 0

Query:  192 DEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGEYGKA-----------APDHDEL 384
            DE    R+ EF G+  E++PT+R+I++ E D+ KYKPE+++ S + +E F+K FLD K+  H LS ++PEDWDKNPVK LV  NF+ VA +K K+VLVEFYAPWCGHCKQL PI++++AE     +D+V+AKMDST NEL   K+ SFPTIK F+K DN+ +++N +RTL+   KFL + GE   +           AP  DEL
Sbjct:  294 DEEDHTRIFEFFGMNKEEVPTIRLIKLEE-DMAKYKPESDDLSAETIEAFLKKFLDGKLKQHLLSQELPEDWDKNPVKVLVSSNFESVALDKSKSVLVEFYAPWCGHCKQLAPIYDQLAEKYKDNEDIVIAKMDSTANELESIKISSFPTIKYFRKEDNKVIDFNLDRTLDDFVKFLDANGEVADSEPVEETEEEEEAPKKDEL 496          

HSP 2 Score: 196.052 bits (497), Expect = 9.879e-57
Identity = 89/194 (45.88%), Postives = 133/194 (68.56%), Query Frame = 0

Query:    4 EIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGRAV 197
            E++ +EG+ V    N+ + + + +++LVEF+APWCGHCKALAPEY KAAQ+L E++S IKLAKVD T E EL E+  ++GYPT+ F+R G P++YSGGR A +I  WV KKTGPPA  L S    + ++ D+++A++GFFKD +S+ AK +       + +   ++S+   I   + +D  VVLFK FD+ ++V
Sbjct:   22 EVKVEEGVLVATVDNFKQLIADNEFVLVEFYAPWCGHCKALAPEYAKAAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSGSPVEYSGGRQAADIIAWVTKKTGPPAKDLTSVADAEQFLKDNEIAIIGFFKDLESEEAKTFTKVANALDSFVFGVSSNADVIAKYEAKDNGVVLFKPFDDKKSV 215          

HSP 3 Score: 70.8626 bits (172), Expect = 3.552e-12
Identity = 38/85 (44.71%), Postives = 55/85 (64.71%), Query Frame = 0

Query:  287 VLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMV-VAKMDSTT-NELS-HTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFL 368
            VLVEFYAPWCGHCK L P + + A+ L++K+  + +AK+D+T   EL+    VR +PT+K F+ G    VEY+G R    I  ++
Sbjct:   47 VLVEFYAPWCGHCKALAPEYAKAAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSG--SPVEYSGGRQAADIIAWV 129          

HSP 4 Score: 70.4774 bits (171), Expect = 4.528e-12
Identity = 38/103 (36.89%), Postives = 67/103 (65.05%), Query Frame = 0

Query:   13 VLNAKNYDK-AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYR--DGEPIDYSGGRMANEIAGWVE 112
            VL + N++  A+ + K +LVEF+APWCGHCK LAP Y + A++ K+ + +I +AK+D T  +  +E + I  +PTI ++R  D + ID++  R  ++   +++
Sbjct:  371 VLVSSNFESVALDKSKSVLVEFYAPWCGHCKQLAPIYDQLAEKYKDNE-DIVIAKMDSTANE--LESIKISSFPTIKYFRKEDNKVIDFNLDRTLDDFVKFLD 470          

BLAST of Calsequestrin-1 precursor vs. SwissProt
Match: gi|2507460|sp|P07237.3|PDIA1_HUMAN (RecName: Full=Protein disulfide-isomerase; Short=PDI; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor)

HSP 1 Score: 211.846 bits (538), Expect = 1.143e-62
Identity = 95/177 (53.67%), Postives = 130/177 (73.45%), Query Frame = 0

Query:  198 RVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLF-KKGDNEAVEYNGERTLEGITKFLASGGE 373
            R++EF GLK E+ P +R+I + E ++ KYKPE+EE + + + +F   FL+ K+  H +S ++PEDWDK PVK LVGKNF++VAF++ KNV VEFYAPWCGHCKQL PIW+++ E     +++V+AKMDST NE+   KV SFPT+K F    D   ++YNGERTL+G  KFL SGG+
Sbjct:  300 RILEFFGLKKEECPAVRLITLEE-EMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQ 475          

HSP 2 Score: 194.897 bits (494), Expect = 3.572e-56
Identity = 93/193 (48.19%), Postives = 133/193 (68.91%), Query Frame = 0

Query:    6 EKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGR 195
            E+++ + VL   N+ +A+  +KYLLVEF+APWCGHCKALAPEY KAA +LK   SEI+LAKVD TEE +L ++  ++GYPTI F+R+G+   P +Y+ GR A++I  W++K+TGP A  L      +  ++ S+VAV+GFFKD +SD AK++  A    ++ P  ITS+       +++   VVLFKKFDEGR
Sbjct:   21 EEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGR 213          

HSP 3 Score: 71.2478 bits (173), Expect = 3.202e-12
Identity = 46/115 (40.00%), Postives = 64/115 (55.65%), Query Frame = 0

Query:  258 DIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFL-SKKDDMVVAKMDST--TNELSHTKVRSFPTIKLFKKGDNEAV-EYNGERTLEGITKFL 368
            D PE+ D   V  L   NF E A    K +LVEFYAPWCGHCK L P + + A  L ++  ++ +AK+D+T  ++      VR +PTIK F+ GD  +  EY   R  + I  +L
Sbjct:   18 DAPEEEDH--VLVLRKSNFAE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWL 129          

HSP 4 Score: 67.781 bits (164), Expect = 3.579e-11
Identity = 44/119 (36.97%), Postives = 69/119 (57.98%), Query Frame = 0

Query:    1 MFQEIEKD---EGIFVLNAKNY-DKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFY---RDGEPIDYSGGRMANEIAGWVE 112
            M QE+ +D   + + VL  KN+ D A  E K + VEF+APWCGHCK LAP + K  +  K+ ++ I +AK+D T  +  +E + +  +PT+ F+    D   IDY+G R  +    ++E
Sbjct:  356 MSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHEN-IVIAKMDSTANE--VEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLE 471          

BLAST of Calsequestrin-1 precursor vs. SwissProt
Match: gi|110815912|sp|Q2HWU2.1|PDIA1_MACFU (RecName: Full=Protein disulfide-isomerase; Short=PDI; AltName: Full=Prolyl 4-hydroxylase subunit beta; Flags: Precursor)

HSP 1 Score: 211.846 bits (538), Expect = 1.555e-62
Identity = 97/190 (51.05%), Postives = 135/190 (71.05%), Query Frame = 0

Query:  185 VVLFKKFDEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKG-DNEAVEYNGERTLEGITKFLASGGE 373
            + +F   D     R++EF GLK E+ P +R+I + E ++ KYKPE++E + + + +F   FL+ K+  H +S ++PEDWDK PVK LVGKNF+EVAF+++KNV VEFYAPWCGHCKQL PIW+++ E     +++V+AKMDST NE+   KV SFPT+K F    D   ++YNGERTL+G  KFL SGG+
Sbjct:  289 LFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEE-EMTKYKPESDELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAIKVHSFPTLKFFPASVDRTVIDYNGERTLDGFKKFLESGGQ 477          

HSP 2 Score: 194.512 bits (493), Expect = 6.149e-56
Identity = 93/193 (48.19%), Postives = 133/193 (68.91%), Query Frame = 0

Query:    6 EKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGR 195
            E+++ + VL   N+ +A+  +KYLLVEF+APWCGHCKALAPEY KAA +LK   SEI+LAKVD TEE +L ++  ++GYPTI F+R+G+   P +Y+ GR A++I  W++K+TGP A  L      +  ++ S+VAV+GFFKD +SD AK++  A    ++ P  ITS+       +++   VVLFKKFDEGR
Sbjct:   23 EEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGR 215          

HSP 3 Score: 71.2478 bits (173), Expect = 2.833e-12
Identity = 47/118 (39.83%), Postives = 65/118 (55.08%), Query Frame = 0

Query:  255 LSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFL-SKKDDMVVAKMDST--TNELSHTKVRSFPTIKLFKKGDNEAV-EYNGERTLEGITKFL 368
            L  D PE+ D   V  L   NF E A    K +LVEFYAPWCGHCK L P + + A  L ++  ++ +AK+D+T  ++      VR +PTIK F+ GD  +  EY   R  + I  +L
Sbjct:   17 LYADAPEEEDH--VLVLRKSNFAE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWL 131          

HSP 4 Score: 67.0106 bits (162), Expect = 7.282e-11
Identity = 43/119 (36.13%), Postives = 70/119 (58.82%), Query Frame = 0

Query:    1 MFQEIEKD---EGIFVLNAKNYDK-AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYR---DGEPIDYSGGRMANEIAGWVE 112
            M QE+ +D   + + VL  KN+++ A  E K + VEF+APWCGHCK LAP + K  +  K+ ++ I +AK+D T  +  +E + +  +PT+ F+    D   IDY+G R  +    ++E
Sbjct:  358 MSQELPEDWDKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHEN-IVIAKMDSTANE--VEAIKVHSFPTLKFFPASVDRTVIDYNGERTLDGFKKFLE 473          

BLAST of Calsequestrin-1 precursor vs. SwissProt
Match: gi|341941239|sp|P09103.2|PDIA1_MOUSE (RecName: Full=Protein disulfide-isomerase; Short=PDI; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Endoplasmic reticulum resident protein 59; Short=ER protein 59; Short=ERp59; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor)

HSP 1 Score: 210.69 bits (535), Expect = 3.538e-62
Identity = 99/199 (49.75%), Postives = 137/199 (68.84%), Query Frame = 0

Query:  185 VVLFKKFDEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLF-KKGDNEAVEYNGERTLEGITKFLASGGEYGKAAPDHD 382
            + +F   D     R++EF GLK E+ P +R+I + E ++ KYKPE++E + + + +F   FL+ K+  H +S ++PEDWDK PVK LVG NF+EVAF++ KNV VEFYAPWCGHCKQL PIW+++ E     +++++AKMDST NE+   KV SFPT+K F    D   ++YNGERTL+G  KFL SGG+ G A  D D
Sbjct:  289 LFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEE-EMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQEVPEDWDKQPVKVLVGANFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDG-AGDDED 485          

HSP 2 Score: 185.267 bits (469), Expect = 1.743e-52
Identity = 92/194 (47.42%), Postives = 134/194 (69.07%), Query Frame = 0

Query:    5 IEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGR 195
            +E+++ + VL   N+++A+  +KYLLVEF+APWCGHCKALAPEY KAA +LK   SEI+LAKVD TEE +L ++  ++GYPTI F+++G+   P +Y+ GR A++I  W++K+TGP A  L      +  +D S+V V+GFFKD +SD AK++  A    ++ P  ITS+       +++   VVLFKKFDEGR
Sbjct:   22 LEEEDNVLVLKKSNFEEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLSDTAAAESLVDSSEVTVIGFFKDVESDSAKQFLLAAEAIDDIPFGITSNSGVFSKYQLDKDGVVLFKKFDEGR 215          

HSP 3 Score: 66.6254 bits (161), Expect = 1.031e-10
Identity = 43/105 (40.95%), Postives = 59/105 (56.19%), Query Frame = 0

Query:  268 VKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKK-DDMVVAKMDST--TNELSHTKVRSFPTIKLFKKGDNEAV-EYNGERTLEGITKFL 368
            V  L   NF+E A    K +LVEFYAPWCGHCK L P + + A  L  +  ++ +AK+D+T  ++      VR +PTIK FK GD  +  EY   R  + I  +L
Sbjct:   28 VLVLKKSNFEE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAAKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWL 131          

HSP 4 Score: 65.4698 bits (158), Expect = 2.015e-10
Identity = 42/119 (35.29%), Postives = 69/119 (57.98%), Query Frame = 0

Query:    1 MFQEIEKD---EGIFVLNAKNYDK-AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFY---RDGEPIDYSGGRMANEIAGWVE 112
            M QE+ +D   + + VL   N+++ A  E K + VEF+APWCGHCK LAP + K  +  K+ ++ I +AK+D T  +  +E + +  +PT+ F+    D   IDY+G R  +    ++E
Sbjct:  358 MSQEVPEDWDKQPVKVLVGANFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHEN-IIIAKMDSTANE--VEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLE 473          

BLAST of Calsequestrin-1 precursor vs. SwissProt
Match: gi|62287145|sp|Q5R5B6.1|PDIA1_PONAB (RecName: Full=Protein disulfide-isomerase; Short=PDI; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor)

HSP 1 Score: 210.305 bits (534), Expect = 5.060e-62
Identity = 94/177 (53.11%), Postives = 130/177 (73.45%), Query Frame = 0

Query:  198 RVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLF-KKGDNEAVEYNGERTLEGITKFLASGGE 373
            R++EF GLK E+ P +R+I + E ++ KYKPE+EE + + + +F   FL+ K+  H +S ++P+DWDK PVK LVGKNF++VAF++ KNV VEFYAPWCGHCKQL PIW+++ E     +++V+AKMDST NE+   KV SFPT+K F    D   ++YNGERTL+G  KFL SGG+
Sbjct:  300 RILEFFGLKKEECPAVRLITLEE-EMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPDDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQ 475          

HSP 2 Score: 196.052 bits (497), Expect = 1.466e-56
Identity = 94/193 (48.70%), Postives = 134/193 (69.43%), Query Frame = 0

Query:    6 EKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGR 195
            E+++ + VL   N+ +A+  +KYLLVEF+APWCGHCKALAPEY KAA +LK   SEI+LAKVD TEE +L ++  ++GYPTI F+R+G+   P +Y+ GR A++I  W++K+TGP A +L      +  ++ S+VAVVGFFKD +SD AK++  A    ++ P  ITS+       +++   VVLFKKFDEGR
Sbjct:   21 EEEDHVLVLRKSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATILPDGAAAESLVESSEVAVVGFFKDVESDSAKQFLQAAEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGR 213          

HSP 3 Score: 70.8626 bits (172), Expect = 3.566e-12
Identity = 46/115 (40.00%), Postives = 64/115 (55.65%), Query Frame = 0

Query:  258 DIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFL-SKKDDMVVAKMDST--TNELSHTKVRSFPTIKLFKKGDNEAV-EYNGERTLEGITKFL 368
            D PE+ D   V  L   NF E A    K +LVEFYAPWCGHCK L P + + A  L ++  ++ +AK+D+T  ++      VR +PTIK F+ GD  +  EY   R  + I  +L
Sbjct:   18 DAPEEEDH--VLVLRKSNFAE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWL 129          

HSP 4 Score: 67.0106 bits (162), Expect = 6.697e-11
Identity = 44/119 (36.97%), Postives = 68/119 (57.14%), Query Frame = 0

Query:    1 MFQEIEKD---EGIFVLNAKNY-DKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFY---RDGEPIDYSGGRMANEIAGWVE 112
            M QE+  D   + + VL  KN+ D A  E K + VEF+APWCGHCK LAP + K  +  K+ ++ I +AK+D T  +  +E + +  +PT+ F+    D   IDY+G R  +    ++E
Sbjct:  356 MSQELPDDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHEN-IVIAKMDSTANE--VEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLE 471          

BLAST of Calsequestrin-1 precursor vs. SwissProt
Match: gi|129726|sp|P05307.1|PDIA1_BOVIN (RecName: Full=Protein disulfide-isomerase; Short=PDI; AltName: Full=Cellular thyroid hormone-binding protein; AltName: Full=Prolyl 4-hydroxylase subunit beta; AltName: Full=p55; Flags: Precursor)

HSP 1 Score: 210.305 bits (534), Expect = 5.399e-62
Identity = 94/177 (53.11%), Postives = 130/177 (73.45%), Query Frame = 0

Query:  198 RVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLF-KKGDNEAVEYNGERTLEGITKFLASGGE 373
            R++EF GLK E+ P +R+I + E ++ KYKPE++E + + + +F   FL+ K+  H +S ++P+DWDK PVK LVGKNF+EVAF++ KNV VEFYAPWCGHCKQL PIW+++ E     +++V+AKMDST NE+   KV SFPT+K F    D   ++YNGERTL+G  KFL SGG+
Sbjct:  302 RILEFFGLKKEECPAVRLITLEE-EMTKYKPESDELTAEKITEFCHRFLEGKIKPHLMSQELPDDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQ 477          

HSP 2 Score: 192.2 bits (487), Expect = 3.960e-55
Identity = 92/193 (47.67%), Postives = 135/193 (69.95%), Query Frame = 0

Query:    6 EKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGR 195
            ++++ + VL+  N+D+A+  +KYLLVEF+APWCGHCKALAPEY KAA +LK   SEI+LAKVD TEE +L ++  ++GYPTI F+++G+   P +Y+ GR A++I  W++K+TGP A  L      +  ++ S+VAV+GFFKD +SD AK++  A    ++ P  ITS+       +++   VVLFKKFDEGR
Sbjct:   23 DEEDHVLVLHKGNFDEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWLKKRTGPAASTLSDGAAAEALVESSEVAVIGFFKDMESDSAKQFFLAAEVIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFDEGR 215          

HSP 3 Score: 71.633 bits (174), Expect = 1.995e-12
Identity = 44/105 (41.90%), Postives = 60/105 (57.14%), Query Frame = 0

Query:  268 VKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFL-SKKDDMVVAKMDST--TNELSHTKVRSFPTIKLFKKGDNEAV-EYNGERTLEGITKFL 368
            V  L   NFDE A    K +LVEFYAPWCGHCK L P + + A  L ++  ++ +AK+D+T  ++      VR +PTIK FK GD  +  EY   R  + I  +L
Sbjct:   28 VLVLHKGNFDE-ALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPKEYTAGREADDIVNWL 131          

HSP 4 Score: 66.2402 bits (160), Expect = 1.279e-10
Identity = 43/119 (36.13%), Postives = 69/119 (57.98%), Query Frame = 0

Query:    1 MFQEIEKD---EGIFVLNAKNYDK-AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFY---RDGEPIDYSGGRMANEIAGWVE 112
            M QE+  D   + + VL  KN+++ A  E K + VEF+APWCGHCK LAP + K  +  K+ ++ I +AK+D T  +  +E + +  +PT+ F+    D   IDY+G R  +    ++E
Sbjct:  358 MSQELPDDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHEN-IVIAKMDSTANE--VEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLE 473          

BLAST of Calsequestrin-1 precursor vs. nr
Match: gi|242008934|ref|XP_002425249.1| (Calsequestrin-1 precursor, putative [Pediculus humanus corporis] >gi|212508997|gb|EEB12511.1| Calsequestrin-1 precursor, putative [Pediculus humanus corporis])

HSP 1 Score: 402.905 bits (1034), Expect = 1.625e-135
Identity = 193/378 (51.06%), Postives = 266/378 (70.37%), Query Frame = 0

Query:    3 QEIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKF-------DEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGE 373
             EIE DEG+ VLN  N+  A+ + +++LVEF+APWCGHCKALAPEY KAA+ L+E  S+IKLAKVD TEE EL E+ ++KGYPT+ F++ G  ++YSG ++A++I  W++KKTGPPA  LES +  K +ID+++V VVGFFKD  SD AK +       +     ITSD     +  V+  ++VLFKK        D+   +R++EF G+K    P++R+I++ E ++ KYKP  ++   DN+ +FV+DFL   +  H L  D+PEDW+K PV TLV  NFDEVAF+  K+VLVEFYAPWCGHCKQL PI++++ E     D +V+AKMD+T NEL HTK+ SFPT+KL+K GDN+ V+Y+GERTLE + KF+  GGE
Sbjct:   20 SEIETDEGVLVLNKNNFQSAISDVEFILVEFYAPWCGHCKALAPEYAKAAKLLEEEGSKIKLAKVDATEETELAEQHNVKGYPTLKFFKKGHVVEYSG-KVADQIVSWLKKKTGPPAKNLESVDDAKAFIDENNVVVVGFFKDQSSDNAKAFLQTAEVMDNVVFGITSDSAVFTEYSVDGDKIVLFKKVLFVSINTDDDDHLRILEFFGMKKTDTPSMRLIKLEE-EMAKYKPVDDKVEPDNVRKFVEDFLSGNLKQHLLCQDLPEDWNKTPVHTLVASNFDEVAFDSSKDVLVEFYAPWCGHCKQLAPIYDQLGEHFKDDDKVVIAKMDATANELEHTKISSFPTLKLYKSGDNKVVDYSGERTLEALIKFIELGGE 395          

BLAST of Calsequestrin-1 precursor vs. nr
Match: gi|1355995932|gb|PRD35987.1| (Pdi [Nephila clavipes])

HSP 1 Score: 385.571 bits (989), Expect = 9.221e-129
Identity = 202/377 (53.58%), Postives = 257/377 (68.17%), Query Frame = 0

Query:    4 EIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGEYGKAAPD 380
            EI+K+  + VL   N++ AVK  K +LVEF+APWCGHCKAL PEY KAA+ L    S++ LAKVD T E EL E+  ++GYPTI F+R+G+ ++Y+GGR A++I  W++KKTGPPA  L SA+  K ++D+++V VVGFFKD +   AK YK    + +++   ITSD+    + K     VVLFKKFDEGR     EF G  NE+                     ++F K N    V +F  ++   H LS+D+PE WDK PVK LVGKNFDEVAF+K KNVLVEFYAPWCGHCKQL PI++E+AE      D+V+AKMD+T NEL HTK+ SFPTIKL+KKG NE VEYNGERTLEGI KF+ + GEYGKAAPD
Sbjct:   19 EIKKENHVLVLTKDNFEGAVKN-KNVLVEFYAPWCGHCKALEPEYAKAAETLDAEKSDLFLAKVDATSETELAEQHGVRGYPTIKFFREGKVMEYTGGRTADDIIRWLKKKTGPPATDLTSADAAKQFVDNNEVVVVGFFKDQEGAEAKIYKNVAAEMDDFSFGITSDDGVYSELKASKDGVVLFKKFDEGRN----EFDGELNEE-------------------NLKKFLKSNSLPLVVEFSHEQ---HLLSEDVPEGWDKEPVKVLVGKNFDEVAFDKSKNVLVEFYAPWCGHCKQLAPIYDELAEKYKDSADVVIAKMDATANELEHTKINSFPTIKLYKKGTNEVVEYNGERTLEGINKFIETDGEYGKAAPD 368          

BLAST of Calsequestrin-1 precursor vs. nr
Match: gi|32566189|ref|NP_872239.1| (Protein Disulfide Isomerase [Caenorhabditis elegans] >gi|351049609|emb|CCD63284.1| Protein Disulfide Isomerase [Caenorhabditis elegans])

HSP 1 Score: 357.451 bits (916), Expect = 2.012e-117
Identity = 178/399 (44.61%), Postives = 257/399 (64.41%), Query Frame = 0

Query:    5 IEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKY-----KAAVRDYEEYPCAITSD---------------------EQAIKDNKVEDGQVVLFKKF--DEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGEYG 375
            IE++E + VL   N+D+ +   +++LVEF+APWCGHCK+LAPEY KAA +LKE  S+IKL K+D T   E+  K  ++GYPT+  +R+G+P +Y+GGR  + I  W++KKTGP A  L  A+ VK+  + +DV V+G+FKD  SD AK +      A V ++ +   ++                        EQ  K+   +    VLF     D     R++EF GLK +++P +R+I + E D+ K+KP+ EE + +N+ +F +++LD  V  H +S+DIPEDWDKNPVK LVGKNF++VA +  KNVLVEFYAPWCGHCKQL P W+++ E  +  + +V+AKMDST NE+   K++SFPTIK F  G N+ V+Y G+RT+EG TKFL + G+ G
Sbjct:   19 IEEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADVVVIGYFKDTTSDDAKTWIQANRLALVSEFTQETASVIFGGEIKSHNLLFVSKESSEFAKLEQEFKNAAKQFKGKVLFVYINTDVEENARIMEFFGLKKDELPAIRLISLEE-DMTKFKPDFEEITTENISKFTQNYLDGSVKPHLMSEDIPEDWDKNPVKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLETNGKEG 416          

BLAST of Calsequestrin-1 precursor vs. nr
Match: gi|193209454|ref|NP_001123100.1| (Protein Disulfide Isomerase [Caenorhabditis elegans] >gi|351049608|emb|CCD63283.1| Protein Disulfide Isomerase [Caenorhabditis elegans])

HSP 1 Score: 354.369 bits (908), Expect = 3.117e-117
Identity = 170/371 (45.82%), Postives = 245/371 (66.04%), Query Frame = 0

Query:    5 IEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGEYG 375
            IE++E + VL   N+D+ +   +++LVEF+APWCGHCK+LAPEY KAA +LKE  S+IKL K+D T   E+  K  ++GYPT+  +R+G+P +Y+GGR  + I  W++KKTGP A  L  A+ VK+  + +DV V+G+FKD  SD AK +                    +++N                   R++EF GLK +++P +R+I + E D+ K+KP+ EE + +N+ +F +++LD  V  H +S+DIPEDWDKNPVK LVGKNF++VA +  KNVLVEFYAPWCGHCKQL P W+++ E  +  + +V+AKMDST NE+   K++SFPTIK F  G N+ V+Y G+RT+EG TKFL + G+ G
Sbjct:   19 IEEEENVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADVVVIGYFKDTTSDDAKTF--------------------LEEN------------------ARIMEFFGLKKDELPAIRLISLEE-DMTKFKPDFEEITTENISKFTQNYLDGSVKPHLMSEDIPEDWDKNPVKILVGKNFEQVARDNTKNVLVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDVKIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKFLETNGKEG 350          

BLAST of Calsequestrin-1 precursor vs. nr
Match: gi|260821328|ref|XP_002605985.1| (hypothetical protein BRAFLDRAFT_126559 [Branchiostoma floridae] >gi|229291322|gb|EEN61995.1| hypothetical protein BRAFLDRAFT_126559 [Branchiostoma floridae])

HSP 1 Score: 355.91 bits (912), Expect = 6.475e-116
Identity = 192/440 (43.64%), Postives = 268/440 (60.91%), Query Frame = 0

Query:    7 KDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVG------------FFKDADSD-------------------------LAKKYKAAVRDYE---------------EYPCAIT---SDEQAIKDNKVEDGQVVLFKK-----------FDEGRAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKG-DNEAVEYNGERTLEGITKFLASGGEYGKAAP 379
            ++E + VL   N++ A+ E++ +LVEF+APWCGHCKALAPEY KAA  LKE++S IKLAKVD T E ++ +K  ++GYPT+ F+R+G+P++Y GGR A++I  W+EKKTGPPA  LE+A++ +   +D++V VVG            F + A SD                         L KK+     D+E               + P  +    S  Q +   +V++  ++   K            D     R++EF GL  E+ P +R+I + E D+ KYKPETEE + +N++ FV+ F+DK +    +S D+PEDWDK  VK LVGKNF EVA +++K VLVEFYAPWCGHCKQL PI++E+ E     +D+VVAKMDST NE+   K++SFPTIK F KG D++ V+YNGERTLE + KFL SGG+ G A P
Sbjct:   22 EEEDVLVLTNDNFEAAIAEFENILVEFYAPWCGHCKALAPEYAKAAGSLKEKESAIKLAKVDATVESDIAQKFEVRGYPTMKFFRNGKPMEYGGGRQADQIVTWLEKKTGPPAANLETADQAEKLKEDNEVLVVGFFKDQESDGAKAFLEVARSDDETTFAITSTDEVYTKLEAKGDGVVLFKKFDEGRNDFEGEVNEDGLKQFIKENQLPLVVEFTESTAQKVFGGEVKNHNLLFISKEHEEILFIYINVDNDDHSRILEFFGLNKEECPQVRLISLDE-DMTKYKPETEEITTENMKAFVQGFIDKTIKAFLMSQDVPEDWDKEGVKVLVGKNFAEVALDENKAVLVEFYAPWCGHCKQLAPIYDELGEKFKDSEDIVVAKMDSTANEVEDVKIQSFPTIKYFPKGKDSQVVDYNGERTLEAMAKFLESGGKDG-AGP 459          

HSP 2 Score: 75.0998 bits (183), Expect = 5.217e-11
Identity = 46/117 (39.32%), Postives = 69/117 (58.97%), Query Frame = 0

Query:  255 LSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMV-VAKMDST--TNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFL 368
            L+ D  E+ D   V  L   NF E A  + +N+LVEFYAPWCGHCK L P + + A  L +K+  + +AK+D+T  ++     +VR +PT+K F+ G  + +EY G R  + I  +L
Sbjct:   16 LAGDYSEEED---VLVLTNDNF-EAAIAEFENILVEFYAPWCGHCKALAPEYAKAAGSLKEKESAIKLAKVDATVESDIAQKFEVRGYPTMKFFRNG--KPMEYGGGRQADQIVTWL 126          

BLAST of Calsequestrin-1 precursor vs. nr
Match: gi|914572922|gb|KOB75632.1| (Protein disulfide-isomerase [Operophtera brumata])

HSP 1 Score: 348.977 bits (894), Expect = 4.740e-114
Identity = 183/404 (45.30%), Postives = 252/404 (62.38%), Query Frame = 0

Query:    4 EIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGRAVRVVEFL---GLKN----EKMPT---------------------LRIIQMTEMDVVKY----KPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNEAVEYNGERTLEGITKFLASGGEYG 375
            EIE DE + VL   N++ A+   +++LVEF+APWCGHCK+LAPEY KAA +L E +S IKLAKVD T+EQEL E   ++GYPT+ F+++G PIDYSGGR A++I  W++KKTGPPA+ + SAE+ K+ I  ++V V GFF D ++D AK +       ++   AI SDE+ I + + ED      + F+E R   V E      LK     + MP                      L  +     D  KY    KP  + +    + +F++ F    +  H LS+D+P DW+  PVK LV  NFDEV F+ +K VLVEFYAPWCGHCKQLVPI++++AE     +D+V+AK+D+T NEL HTK+ SFPTIKL+ K DN+  +YNGERTL+G+TKF+ + G  G
Sbjct:   22 EIETDENVLVLTKSNFESALSSSQFILVEFYAPWCGHCKSLAPEYAKAATKLLEEESPIKLAKVDATQEQELAESYGVRGYPTLKFFKNGSPIDYSGGRQADDIVSWLKKKTGPPAVEVSSAEQSKELIAANNVIVFGFFADQETDKAKAFLNVAGLVDDQVFAIVSDEKLISELEAED------QDFEEKRVKYVEEAFNEDALKTWVFVQSMPVIVEFSHETASKIFGGQIKYHLLLFLSKKNGDFEKYLDDLKPVAKNYRDKIMSEFIQSFFAGTLKQHLLSEDLPSDWNAKPVKVLVATNFDEVVFDTNKKVLVEFYAPWCGHCKQLVPIYDKLAEHFESDEDVVIAKIDATVNELEHTKITSFPTIKLYTK-DNQVKDYNGERTLDGMTKFVETDGADG 418          

BLAST of Calsequestrin-1 precursor vs. nr
Match: gi|966651017|gb|KTF85919.1| (hypothetical protein cypCar_00037209, partial [Cyprinus carpio])

HSP 1 Score: 335.495 bits (859), Expect = 4.212e-109
Identity = 182/411 (44.28%), Postives = 250/411 (60.83%), Query Frame = 0

Query:    4 EIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADSDLAKKYKAAVRDYEEYPCAITSDEQAIKDNKVEDGQVVLFKKFDEGRAV--------RVVEFLGLKNEKMPTLRIIQMTEMD------------VVKYKPETEEFSKDNLEQFVKDFLDKKVPV-----------------------HYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDN-EAVEYNGERTLEGITKF 367
            +I ++E + VL   N+++A+K Y  +LVEF+APWCGHCKALAPEY KAA  LK   ++I+LAKVD TEE EL ++  ++GYPTI F++ GE   P +YS GR A++I  W++K+TGP A  L    + +  I D++VAV+GFFKD +S+ AK +       ++ P  I S++      +V    VVLFKKFDEGR           ++ F  +K  ++P   +I+ TE              ++ + P+T    ++ ++QF K     K  V                       H +S DIPEDWDKNPVK LVGKNF+EVAF+  KNV VEFYAPWCGHCKQL PIW+++ E    K ++VVAKMDST NE+   KV SFPT+K F  GD+ + ++YNGERTL+G TKF
Sbjct:   17 DIPEEEDVLVLKKSNFEEALKTYPNILVEFYAPWCGHCKALAPEYAKAAGMLKAESTDIRLAKVDATEESELAQEFGVRGYPTIKFFKGGEKENPKEYSAGRQADDIVNWLKKRTGPAATTLSDVTQAESLIADNEVAVIGFFKDVESEDAKVFIKTAEAVDDIPFGIVSNDAVFSKFEVAKDGVVLFKKFDEGRNTFDGEISKENLLNF--IKANQLPL--VIEFTEQTAPKIFGGEIKSHILMFVPKTATDFQEKMDQFKKASEGFKGKVENVFCVFGMIVVCVLNDCNVFQPHLMSQDIPEDWDKNPVKVLVGKNFEEVAFDPAKNVFVEFYAPWCGHCKQLAPIWDQLGEKFKDKANIVVAKMDSTVNEIEAVKVHSFPTLKFFPAGDDRKVIDYNGERTLDGFTKF 423          

BLAST of Calsequestrin-1 precursor vs. nr
Match: gi|927153111|ref|XP_013916852.1| (PREDICTED: protein disulfide-isomerase [Thamnophis sirtalis])

HSP 1 Score: 331.257 bits (848), Expect = 4.099e-106
Identity = 187/460 (40.65%), Postives = 271/460 (58.91%), Query Frame = 0

Query:    4 EIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYID-------------DSDVAV-----------VGFFKDADSDLAKKYK------AAVRDYEE----YPCAITSDE--QAIKDNKVE---------------------------------DGQVVLFKKFDEG---------------RAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKG-DNEAVEYNGERTLEGITKFLASGGEYG 375
            ++E++EG+ VL + N+++A+++Y  +LVEF+APWCGHCKALAPEYVKAA +LK  +SEI+LAKVD TEE EL ++  ++GYPTI F+++G+   P +Y+ GR A++I  W++K+TGP A  L      ++ ++             +SDVA            + F   ++SDL  KY+      A  + ++E    +   IT D     IK N++                                  +G++  FKK  EG                  R++EF GLK E+ P +R+I + E ++ KYKPE+++ S +N+  F   FLD KV  H +S +I +DWDK PVK LVGKNF+EVAF+++KNV VEFYAPWCGHCKQL PIW+++ E     +++++AKMDST NE+   KV SFPT+K F  G D   ++Y+GERTLEG  KFL SGG+ G
Sbjct:   24 DLEEEEGVLVLKSANFEQALEQYPNILVEFYAPWCGHCKALAPEYVKAAAKLKSENSEIRLAKVDATEESELAQQFGVRGYPTIKFFKNGDKSSPKEYTAGREADDILNWLKKRTGPAATTLADVAAAEELVESSEVAVIGFFKDAESDVAKEFLLAAEAIDDIPFGITSNSDLFAKYQLKKDGIALFKKFDEGRNNFEGEITKDNLLNFIKSNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVEEYEGKLDNFKKAAEGFKGKILFIYIDSDHSDNQRILEFFGLKKEECPAIRLITLEE-EMTKYKPESDDLSPENIRDFCHKFLDGKVKPHLMSQEISDDWDKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIIIAKMDSTANEVEAVKVHSFPTLKYFPAGPDRTVLDYSGERTLEGFKKFLESGGKDG 482          

HSP 2 Score: 66.6254 bits (161), Expect = 3.134e-8
Identity = 42/109 (38.53%), Postives = 65/109 (59.63%), Query Frame = 0

Query:    1 MFQEIEKD---EGIFVLNAKNYDK-AVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDG---EPIDYSGGR 102
            M QEI  D   + + VL  KN+++ A  E K + VEF+APWCGHCK LAP + K  +  K+ ++ I +AK+D T  +  +E + +  +PT+ ++  G     +DYSG R
Sbjct:  361 MSQEISDDWDKQPVKVLVGKNFEEVAFDENKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHEN-IIIAKMDSTANE--VEAVKVHSFPTLKYFPAGPDRTVLDYSGER 466          

BLAST of Calsequestrin-1 precursor vs. nr
Match: gi|780121170|ref|XP_011677933.1| (PREDICTED: protein disulfide-isomerase 2-like [Strongylocentrotus purpuratus])

HSP 1 Score: 315.849 bits (808), Expect = 2.058e-101
Identity = 167/387 (43.15%), Postives = 241/387 (62.27%), Query Frame = 0

Query:    4 EIEKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGEPIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDADS-------------DLAKKYKAAVRDYEEYPCAITSDE-QAIKDNKVEDGQV----VLFKKFDEG--RAVRVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLFKKGDNE--AVEYNGERTLEGITKFL 368
            E+E +E + VL    +   V E +++LVEF+APWCGHCK+LAP+Y  AA+ LK+  S IKLAKVD T E +L  K  ++GYPT+ F+R G+  +Y+GGR   EI  W+ KKTGPPA  + S E  + ++ D +VAV+GFFKD                  +K +   ++ +        SD+ + I D            VLF   D       R++E+ GL +E++PT+R+I + + D+ KYKP   E + ++L QFV DF D K+  H +S+ +PEDW+ NPV  LVG+NF EVA +  K+VLVEFYAPWCGHCKQL PI+EE+ E   +++D+V+AK+DST NE+    VRSFPT+K +KKG+NE  + +Y+ +R   G+T  +
Sbjct:   20 EVEIEEDVAVLTDAAFADYVAENEFVLVEFYAPWCGHCKSLAPQYSIAAKTLKDSGSSIKLAKVDATVETQLAGKYGVRGYPTLKFFRSGKDSEYAGGRTGPEIVAWLNKKTGPPAATIASVEDAEAFLADKEVAVIGFFKDVPQVRNLTGCSLLYKVTASKIFGGEIKIHNLLFVKKDSDDFKTIYDQFYAAATTFKGEVLFVLIDAAAESNSRILEYFGLGDEEVPTVRLITL-DGDMKKYKPTVPELTTESLSQFVIDFKDGKLKPHLMSESVPEDWNANPVTILVGENFAEVALDPTKDVLVEFYAPWCGHCKQLAPIYEELGEHFKEREDVVIAKVDSTKNEVEDAVVRSFPTLKFWKKGENEGTSTKYSPQRV--GLTHLV 403          

BLAST of Calsequestrin-1 precursor vs. nr
Match: gi|634829293|ref|XP_007957767.1| (PREDICTED: protein disulfide-isomerase isoform X2 [Orycteropus afer afer])

HSP 1 Score: 315.464 bits (807), Expect = 9.213e-101
Identity = 174/410 (42.44%), Postives = 250/410 (60.98%), Query Frame = 0

Query:    6 EKDEGIFVLNAKNYDKAVKEYKYLLVEFHAPWCGHCKALAPEYVKAAQELKERDSEIKLAKVDGTEEQELMEKMHIKGYPTIFFYRDGE---PIDYSGGRMANEIAGWVEKKTGPPALVLESAEKVKDYIDDSDVAVVGFFKDA----DSDLAKKYKAAVRDYEEYPCAITSDEQA--------IKDNKV---------EDGQVVLFKKFDE---GRAV------------RVVEFLGLKNEKMPTLRIIQMTEMDVVKYKPETEEFSKDNLEQFVKDFLDKKVPVHYLSDDIPEDWDKNPVKTLVGKNFDEVAFNKDKNVLVEFYAPWCGHCKQLVPIWEEVAEFLSKKDDMVVAKMDSTTNELSHTKVRSFPTIKLF-KKGDNEAVEYNGERTLEGITKFLASGGEYG 375
            E+++ + VL   N+++A+  +KYLLVEF+APWCGHCKALAPEY KAA +LK   SEI+LAKVD TEE +L ++  ++GYPTI F+++G+   P +Y+ GR A++I         P  +   S    K  +D   VA+   F +     + ++ K+   +   + + P  I   EQ         IK + +          DG++  FKK  E   G+ +            R++EF GLK E+ P +R+I + E ++ KYKPE+ E + D +++F   FL+ K+  H +S ++PEDWDK PVK LVGKNF+EVAF++ KNV VEFYAPWCGHCKQL PIW+++ E     +++V+AKMD+T NE+   KV SFPT+K F    D   ++YNGERTLEG  KFL SGG+ G
Sbjct:   23 EEEDHVLVLKKSNFEEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFKNGDTASPREYTAGREADDI---------PFGITSNSDVFSKYQLDKDGVALFKKFDEGRNNFEGEVTKENLLSFIRHNQLPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSSFKKAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEE-EMTKYKPESAELTADKIKEFCDQFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEEVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDATANEVEAVKVHSFPTLKFFPASADKTVIDYNGERTLEGFKKFLESGGQDG 422