BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000010075 (supercontig:LSalAtl2s:LSalAtl2s663:686266:692950:1 gene:EMLSAG00000010075 transcript:EMLSAT00000010075 description:"maker-LSalAtl2s663-augustus-gene-7.9")

HSP 1 Score: 858.596 bits (2217), Expect = 0.000e+0
Identity = 494/1034 (47.78%), Postives = 671/1034 (64.89%), Query Frame = 0

Query:    1 MERVPLMTMNTSN-----------------KVCAQLSCQDAQSGVLDALRRDDPTTFSSILAEECHLDDTIFLRGSLSDDEKKKLLLPLDHWINQPILEEGEKTLLQVALQHE----NVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTD-LVGT-KSVKEAIKENLPYFDVSQVVVKEKPRENTLDYLMEMLERRDLAYFKAVLRFLEPSEVSTSGVRG-FTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHED-FSVTFHYDFLAPPIPEEQLEEWDDEQQSRLEKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMRCSSCSKCAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKG------PLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWA-----PVSW-ARMCPSGTLLYHLYNSCPALKRLSHKITGATGILLFFSYLPTKQETFP 997
            MER+PLM    SN                   C QLSC+D Q G+   L   D TTFSSILAE   +        + ++      +LP   WIN  I E  ++ +LQ+AL+ E     + F + LL  GA A+ +N E D A IH AVQS NL+ V ALLE  +NK D ++  QP GQ A+H+A  +    IL RLL+E KA +NAKDN G +TPL LA   +N E+  LL+E+GA+L + +VG  ++++  ++   P   + ++ +K++P   TLD+L ++L++RDL  FK+ +RFL+ SE+S   VRG  T++Q+A IEGL+++V VL++ G ++N  +KEN + P +LAA+RGH+ VLE    Y  K +       +  WT D++ET+LH+IL+KS R      + + + I      Y K L +L++  S  F  QL+ VINKKD++GN+ +H+AA+ W Q+   +L++LGANIG RN  G++PL +I  +TL +FL+  C    +  DP+ + D F VTF YDFLAPP+ EEQL+ WD E+Q++LE EALPETD++WY+++SK HRHLIKHPV+TSF+WLKWQ+IRR FNRN+RFY  FVV LTWYIFARFGG SL +RGD                  FCS +++ E SY GF+Y++FALH  VQ+     DW ++      + C   +LGSW E+F+  +  + L+ ++G+L  VL + L ++ LRE FQM SS KRY L +ENW+E++LI ++ +ILF        +  + C   RH+ A+ALL++WAELITMVGRHP+LARYNIYVTMFYKVLE+FF+FL+W+SFF+IAF  GFYIMLH+D        G+  D G+ YEFF+SPWL L KT TMFVGEIEFSDIP++          L  I YLFLLAFVF IVVVLMNLLNGLA++DTG+IQ  AEIVS ++RVE++SYAES+LLGDPFDFLS  +     P SW  R  PS  LL  LY   P ++    KITGATGILLFF+ L  K   FP
Sbjct:    1 MERLPLMGKMNSNCSLTKPKEFAPAQDEIPLYCTQLSCKDPQVGLWKCLTNGDLTTFSSILAEVAEV-----CGNNNNNGYSTPSILPSSXWINSKIYE-NDRNILQLALELEPPEKGIPFVKKLLDAGANADFFNVETDLALIHVAVQSNNLKKVEALLELEWNKGDISVTTQPEGQNALHLA--VNNPGILRRLLKEPKARINAKDNCGSRTPLQLALLAKNRESTILLIENGANLEEKVVGDGRNLRNLVQSTFPDLSIDKINIKKQPIHYTLDFLSDVLDKRDLTLFKSAIRFLDKSEISAGTVRGGVTLLQRAAIEGLNEFVCVLINNGADANMYSKENLYTPTILAASRGHYQVLESL-LYKGKAI-------IATWTPDSQETLLHVILRKSLRTG----HASDQSI------YEKCLNVLLE--SEDFCTQLKPVINKKDDLGNTCLHFAAKSWPQKVTLKLLELGANIGIRNHFGEVPLSRIQTKTLEEFLDNYCTQTKK--DPDDYSDSFEVTFRYDFLAPPVTEEQLQNWDREKQTQLELEALPETDSLWYIARSKTHRHLIKHPVLTSFMWLKWQKIRRLFNRNLRFYLLFVVLLTWYIFARFGGKSLNIRGDGE---------------DFCSELRFDE-SYFGFFYILFALHVLVQIGFVITDWYKDYYNDGFTSC---ILGSWLEIFMFLVFAIVLYGSLGLLKWVLVLLLGVMVLREGFQMLSSFKRYLLSVENWMEIFLIGILSVILF-------GNSVVPCKWNRHLAAIALLISWAELITMVGRHPKLARYNIYVTMFYKVLESFFYFLIWFSFFIIAFAFGFYIMLHRD-------NGDNVDKGD-YEFFDSPWLTLAKTFTMFVGEIEFSDIPVDRSTDESEYIALPVIAYLFLLAFVFFIVVVLMNLLNGLAITDTGIIQARAEIVSNVTRVESVSYAESVLLGDPFDFLSSSSANGDGPFSWLNRHLPSCALLNALYTKSPTVRSAFQKITGATGILLFFTLLTDKSARFP 970          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000008528 (supercontig:LSalAtl2s:LSalAtl2s5267:1317:4585:-1 gene:EMLSAG00000008528 transcript:EMLSAT00000008528 description:"maker-LSalAtl2s5267-snap-gene-0.3")

HSP 1 Score: 680.248 bits (1754), Expect = 0.000e+0
Identity = 435/1077 (40.39%), Postives = 599/1077 (55.62%), Query Frame = 0

Query:    2 ERVPLMTMNTSNKVCAQLSC-QDAQSGVLDALRRDDPTTFSSILAEECHLDDTIFLRGSLSDDEKKKLLLPLDHWINQPILEEGEKTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIA--SDLGKLDILERLLQESKAN-VNAKDNKGHQTPLFLAA-KRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQ----VVVKEKPRENTLDYLM---EMLER------RDLAYFKAVLRFLEP--SEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPPI-PEEQLEEWDDEQQSRLE-KEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSY-LGFWYVVFALHSCVQVVLAYRDWTREMRCSS--------CSKCAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGILLFFSYLPTKQETFPIRRRSHKHNRGRYDDDEEVEEEDCCPCCHQDDGNYEDVLKSARAILLQRT 1047
            + VPL  M    + C   SC  + +   + +  + D   F+ ILAE                   KK   P  HWIN+       K+ L +AL+  N +F   LL  GA A+ YN+ L    +H A+++GN  + V+L++     AD N+ +   G + +H A  SD G +DI   L+   K + ++A+D  G +TPL+LA  + +N    + LL+ GA     V  K +K+AI+   P   + +     +V+ K     L+ L+   +M +R      ++L +FK +L+ +    SE++     G T +QK+   GL  +            +   E+S R  + + ++  F                              E I+H ILKKS R              R D  Y     I++   S     +L L+IN  D +GN P+HYA Q W QE V++L++LGANIG  N  G+ P+ KI PETL +F +E  +   Q      +EDF +TF Y FLAPPI  +E  +  +DE  +  E +  LPET+++W++SQSK HRHL+KHPVITSFLWLKWQRIR +FNRN+RFY  FVVALTWYI  RFGG SL+            VGI  S    +CS + + +    L FW+ +F  HS +Q+ L  RDW  E+   S         S     +LGSW E   L +I + L+     LW VL+  +VLL LRELFQ+  SLKRY    ENW+E+ +I LV +ILF+PDS     LE  C+ KRH+ A+ +LL+W+E+IT++ RHPRL RYNIYVTMFYKVL+TFFFFLLWYSFF+ AF LGFYIMLH+D    +L   E       Y FFN PWL+LVKTSTMFVGEIEF+DIPIN+   L+P+ Y FLL+FVFLI+VVLMNLLNGLAVSDTGLI+ +AEIVS++SRVETISY E +LLGDPFDFLS W         P+      L+ + P  +++S+ +TGATGILLF+ YL  K+ T         ++RGR          D CP     D +  +V+KSA+A ++ + 
Sbjct:    7 DSVPLADMK-KGRFCRTTSCFGELEENAIRSFLQKDKLRFNEILAEVS----------------PKKRSHP-HHWINEGHQRLKFKSFLHIALEDRNEEFVNLLLRAGARADTYNEVLGVGLLHVAIEAGNYSNFVSLVKY---GADVNL-VDRKGLSPLHYAVQSD-GNVDIFNSLIHNEKVDELDAEDLAGRRTPLYLAVVQAKNRYFAEALLKRGADSNITVHGKPIKQAIQSTWPSLRIPEKSNSSIVESKELLYGLNRLLDKAQMNKRSKESNAQNLLHFKIILQKMSEMVSEINKYNCGGMTFIQKSSEYGLPTF------------HLNDEDSCRKRIFSTSKDVFG----------------------------GENIIHWILKKSQR-------------DRPDADYQDCASIIL--LSKDLDDELSLIINDADELGNVPLHYATQIWGQEVVKRLLELGANIGIINQWGERPIDKILPETLEEFFDESGL---QSKYDVXNEDFEITFDYSFLAPPILRQETTKRAEDENHNACEERPVLPETESLWHMSQSKNHRHLLKHPVITSFLWLKWQRIRGYFNRNLRFYLLFVVALTWYIVERFGGLSLR----------KNVGIA-SNSTSYCSDLSYRDSDQGLSFWFWIFLTHSFIQIFLMIRDWRYEISTFSRRKGKPGFGSSVLRLILGSWLEAVTLCIIFIVLYFRTAALWSVLTGLIVLLILRELFQLGVSLKRYIFTPENWLEVSMITLVCVILFVPDSYF---LE-PCETKRHLAAITILLSWSEMITLIARHPRLERYNIYVTMFYKVLKTFFFFLLWYSFFIFAFALGFYIMLHKDIPGYDLKNDE-------YTFFNYPWLSLVKTSTMFVGEIEFADIPINVNSKLSPLSYAFLLSFVFLILVVLMNLLNGLAVSDTGLIRAKAEIVSFVSRVETISYTEXLLLGDPFDFLSSWPSFKLLMALPNLACCRLLFQN-PFFRKISYNLTGATGILLFYRYLTDKKLTLT------PNHRGRM---------DFCPLLSVKDMD-PNVIKSAKAKIIAKN 963          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000010412 (supercontig:LSalAtl2s:LSalAtl2s690:121806:126011:1 gene:EMLSAG00000010412 transcript:EMLSAT00000010412 description:"maker-LSalAtl2s690-augustus-gene-1.7")

HSP 1 Score: 637.876 bits (1644), Expect = 0.000e+0
Identity = 395/1072 (36.85%), Postives = 592/1072 (55.22%), Query Frame = 0

Query:   59 SLSDDEKKKLLLPL--DHWINQPILEEGEKTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAA-KRRNAEAVKLLLEHGASLTDL--VGTKSVKEAIKENLPYFDVSQVVVKEKPRENT------LDYLMEMLERRDLA---YFKAVLRF----LEPSEVSTSGVR---GFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPPIPEEQ--LEEWDDEQQ------SRLE---KEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREM--------RCSSCSKCAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGILLFFSYLPTKQETFPIRRRSHKHNRGRYDDDEEVEEEDCCPC--CHQDDGNYEDVLKSARAILLQRTNPPAQAGDTALMMKIMDKQDTLMKLVELQHEEMRHLRNMIN 1088
            SL D    K   PL  DHWIN PI  E  K+L+ + L+ +  +F   LL  GA A+ +N+     PIH   ++G+LE +  L     N ADPN  M+ +G+T +H+ ++   ++  E LL++ K  V+ KD KG QTPLFLAA KR ++  ++ LL++GA   DL  +G+K+ ++ I  N   +D+        P E++      +  L E++E+  L    Y++ +  F    ++  E   +  R      ++QK C + ++D ++ L +   N +         PV +AA R    +L++             + +       + ETILH++LK+  +R+                    +L+ +++DP     +Q+  ++NKKD +GN+ +H AAQ   QE V++L+QLGANIG +N   ++P+  I P T+ +FLNEDC+      D N H+DF + F Y FLAPP  +    L   DD++       S L+   K ALPE +++W +++SK HRHL+KHPVITSFLW KW RIR +FNRN+RF+  FV   TW+IF  +GG  ++                             +E++Y+ F Y+V+ + +       +RD   ++          S+   C   +L SW E+ +L +I   + +    L   L I LV L  REL  +A S+KRY L  EN +++ LI  + ++LF P        E    + RH+    ++L+WA L+T++G+HP+L RYN+YVTMFYKV++TFF+FL+WY+ F+IAFGLGFYI+LH+          E  +G +SY FF++P L+++KTSTMFVGE+EFSDIPI++ GP +   Y F ++FVFLIVVVLMNLLNGLAVS TG+IQE+AE V Y+SRVETISY ES+LLGDPFDFLS W  + W R  PS + L  +Y +   L++  H++TGATGILLF+S+LP K+ T        K N          E+ + CPC   H  D     ++ SA+ I+L +         + L  K+    D L+K  E Q   M  L N+ N
Sbjct:   46 SLVDSIAGKEDFPLQPDHWINSPIESEDGKSLMIIVLEKKYSEFVALLLKFGARADHFNESYGLYPIHIPSRNGDLESLKLLFRYLINSADPNSVMRINGRTPLHLMAENNFVEGXEXLLRQ-KPKVDIKDKKGGQTPLFLAAFKRADSSIIRSLLKYGAD-PDLKCLGSKTARDLINRN---YDIVLDEDNSSPPESSSKQGVSIQRLGEIVEKLGLGQGNYYQLLNEFQYGLIQYKEEEIADYRFPGNMNLVQKICDQNIEDCLKYLFNNFPNMSPDFTSEGLSPVCIAAQRMSRPILQIL---------ADKKADFTTMNTHSSETILHILLKEGNKRS------------------HDALKFILNDPE--IEQQIRSIMNKKDMLGNTALHLAAQRQDQEVVKRLLQLGANIGMKNIYNEMPVTNIXPSTMEEFLNEDCLL--SKGDVN-HDDFELEFDYSFLAPPYEDLMYGLSPHDDDESMIHIKPSDLKIPYKSALPEMNSLWIMAESKEHRHLLKHPVITSFLWCKWGRIRPYFNRNLRFFMTFVFLTTWFIFENYGGSKIK-----------------------------NEQNYITFCYLVYIVFTGCITGFIFRDLNSDINDMMANMKNGSTGRNCLSVLLSSWFEIGLLIVIYTIVILGEPFLTKALFIILVCLLFRELLNLAVSIKRYILSPENLVQVLLIIFMSVLLFDPRRT-----EKHESLHRHIAGTTIVLSWASLVTLIGKHPKLMRYNVYVTMFYKVMKTFFYFLIWYALFIIAFGLGFYIILHK----------ENREGEDSYVFFDTPLLSMIKTSTMFVGELEFSDIPIDIDGPYSTFSYGFFISFVFLIVVVLMNLLNGLAVSXTGIIQEKAETVGYVSRVETISYTESVLLGDPFDFLSNWPKIGWIRSIPSISFLRQMYRN-KYLQKFFHRVTGATGILLFYSFLPDKKLTI-------KPN----------EKLNGCPCFKIHSMD---RSIISSAKKIVLSKKFKEQTVELSELSSKVNSIMDLLLKSEERQKLMMDKLNNIEN 1015          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000004760 (supercontig:LSalAtl2s:LSalAtl2s250:514305:525469:1 gene:EMLSAG00000004760 transcript:EMLSAT00000004760 description:"augustus_masked-LSalAtl2s250-processed-gene-5.0")

HSP 1 Score: 266.159 bits (679), Expect = 1.097e-74
Identity = 157/358 (43.85%), Postives = 218/358 (60.89%), Query Frame = 0

Query:  692 ILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGILLFFSYLPTKQETFPIRRRSHK---HNRGRYDDDEEVEEEDCCPCCHQDDGNYEDVLKSARAILLQR 1046
            IL + LS  L++L  RELFQM  S KRYF  +EN +EL +IAL   I+F  +S     L     + RH+ A+ +L  W EL+ ++GRHP+L+     + MF  V+ TF  FLLWYS  ++AFG+ FY+M+H +             GGE+ +FF S  L+L+KTSTMFVGE+EFSDIP +       + YL  ++F+FLIVVVLMNLLNGLAVSDTGLI+EEAE++ ++SRVE I+Y ES+LLGDPF FLS W  +   +  P   +   LY   P ++ L  K+TG T  LLF+S LP+K+ TF   +R      + R    DDE  +  +             DVL+SA+ ++L++
Sbjct:  629 ILQIFLSGLLLVLFCRELFQMGVSFKRYFFNVENLLELTVIALSITIIFGSNSTPEEIL-----MNRHLAALCVLFTWTELLVLIGRHPKLS---TNIVMFRTVIGTFLTFLLWYSLIIVAFGIAFYLMMHSN-------------GGEN-KFFESVSLSLLKTSTMFVGELEFSDIPFSNNA----LSYLIFVSFIFLIVVVLMNLLNGLAVSDTGLIREEAEVLGWLSRVELITYTESMLLGDPFYFLSNWPAIKLLKKLPHCGVCRILYK-FPFMRDLLQKVTGGTKXLLFYSCLPSKRATFFPNKRGKSLFGYKRSLKIDDESRKAPNLYVST--------DVLESAKXLILEK 951          

HSP 2 Score: 236.884 bits (603), Expect = 8.288e-65
Identity = 181/603 (30.02%), Postives = 299/603 (49.59%), Query Frame = 0

Query:   15 VCAQLSC-QDAQSGVLDALRRDDPTTFSSILAEECHLDDTIFLRGSLSDDEKKKLLLPLDHWINQPILEEGEKTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHI-------ASDLGK---LDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLT-DLVGTKSVKEAIKENLPYFDVSQVVVKEKPRENTLDYLMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLD-AGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILK----KSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSS-AFRRQLELVINKKDNM-GNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPP-IPEEQLEEWDDEQQ----SRLEKEALPETDTIWYLSQ-SKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGG 592
            +C +  C  D Q   LD L      +FS  ++++      I+   +  +D   K LL +   +  P    G    ++  + H N              +  N+ L+ +P+H   + G+ E +  +LE  F   D N+     G T +H+       A D  +   L  +E LL       NA+D +G+ TPL  AA     + ++LLL+ GA    +     ++ + IK  +P FD++  V K K R      L E +E  D+   ++V    E  E+  S     T++Q AC++G D  V  LL   G + N  +      P+L+AA  G++ +L +F+          P+++  +    + +T+LH + +    K  + N++ +  +          Y K  +IL+ D SS  F+ Q++ +IN ++ + G++P+HYA     Q+ +R  ++ GAN+G +N +    +  I P+TL  F +EDCV+        T E F +TF YDFLAPP +    LE ++D+++    S++++  LPET+ +WY+S  SK HR L+KHPVI SFLWLKWQRIR +F  N+  Y  FVV LT +IF ++GG
Sbjct:   44 LCPRAGCLGDPQRAALDYLIFRHFRSFSDAISDDKFDPSYIYENETTDEDRNGKNLLEI-ALLKSP---GGRDRFVETLILHPN----------ATRVDEINELLETSPLHEVARRGDPELLRLVLEAGF-PYDVNIP-DGEGLTPLHVVVESLEEADDAKRTCYLKCMELLLNGEHIEPNAEDLRGNLTPLHYAANIGLQQPIRLLLDRGADPNLEFESGVTISDVIKRKIPEFDLADYVYKIKSRP-LKSRLFEYVENGDIRGLESVKD--EACELWNSDNGSLTLLQMACVKGYDGVVRWLLTVGGADPNARSGGEPRTPILIAAHHGYYRILRIFR-------DEEPRSDFTIIEPVSGKTVLHEVFRQDSAKFIKSNVKSVRGS---------NYRKCFDILLSDTSSKGFKDQIDSIINYREALDGDTPLHYATMQTDQDLIRAFLRRGANMGIKNKKDRTSVQCILPQTLQTFFDEDCVSW---DGIITDEKFKLTFLYDFLAPPKLNNLSLEYFEDQEKNSDPSQMDRRPLPETEPLWYMSMASKEHRMLLKHPVIASFLWLKWQRIRSYFYINLFLYFTFVVLLTAFIFLKYGG 608          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000007091 (supercontig:LSalAtl2s:LSalAtl2s3:842630:846195:1 gene:EMLSAG00000007091 transcript:EMLSAT00000007091 description:"maker-LSalAtl2s3-augustus-gene-8.27")

HSP 1 Score: 244.973 bits (624), Expect = 1.682e-68
Identity = 181/549 (32.97%), Postives = 291/549 (53.01%), Query Frame = 0

Query:   82 EEGEKTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIA-SDLGKLD-----ILERLLQES--KANVNAKDNKGHQTPL--FLAA-----KRRNAEAVKLLLEHGA-SLTDLVGTKSVKEAIKENLPYFDVSQVVVKEKP----------RENTLDYLMEMLERRD----------LAYFKAVLRFLEPSEVSTSGVRG-FTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPN-THEDFSVTFHYDFLAPPIPEEQLEEWDDEQQSRLEKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRF---YAFFVVA--LTWYIF 587
            +E +K LL +A+ + +++  R +LA GA  N  N  L   PIH A++    E +  LL    N ADPN+     G+T +H+A   + K+D      ++ LL+E   K +++A+D+ G Q+PL  F+++       R+ E + L L+ GA S   +V        I EN    +++ +V K K           +E  L  + ++L + D          L  FK  L +L+  +++ S   G  + +Q+AC  GL D+V+VLLD G N N  +   +   V+LA+     +VL++   + +        T+ G   +D  ET+LH IL+K +  +L        E+  ++  +   +EI  + P+     +L  VIN  D + N+P+HYA Q W+Q+ VR L++LG+NIG +N  G+IP+ +I  ETL DF +  C+     H+ N  +ED  +T  YDFLAPP+ ++   E           E LPETD +W++SQ+  HR L+KHPVITSFLW+KW+RI   +N+N+ F   Y+ F++A  L +YI 
Sbjct:   53 QEQQKNLLSIAVDNRDIESLRLILASGANVNAINPLLKTVPIHIAIRKSQPEILRILL---LNNADPNIKTA-DGKTPLHLAIKSMEKVDNEGYACIKTLLREGSKKLSIDAEDSLG-QSPLSAFVSSLNANPTNRDIEIITLFLKSGADSKAKVVNDSRTLAEIIENFHDSELNALVRKNKAEFHPLSSNRVQEGILKDVYDILNKADFSNEAEKSRLLKKFKTTLGYLKDEQITASPPSGVLSPLQRACELGLVDFVQVLLDKGHNPNVHSS-GTTPAVILASKSARSNVLKLLAEHGK--------TDFGA-NKDG-ETVLHAILRKPYSGSLS-------EVMDYENAWETLVEIRSEYPT--INHELSSVINSTDELLNTPLHYATQCWSQKVVRSLLELGSNIGMKNSYGEIPVDRILSETLEDFFSSYCLE----HEGNIANEDLRITVKYDFLAPPVHDKAEGE-----------ELLPETDVLWHMSQNSNHRRLLKHPVITSFLWMKWKRISGSYNKNLLFYFLYSMFIIAFGLGFYIL 561          

HSP 2 Score: 207.608 bits (527), Expect = 6.800e-56
Identity = 111/198 (56.06%), Postives = 135/198 (68.18%), Query Frame = 0

Query:  799 FFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGILLFFSYLPTKQETF 996
             F+FL  YS F+IAFGLGFYI+LH D  S  L   E       Y FF+   L LVKT TMFVGE+EFSD+PI+      P+GYLFL++FVFLIVVV+MNLLNGLAVSDTG+I+EEAEI S + +V+ ISY E++LLGDPF+FLS W    W R  P   L   +Y   PAL+ L  KITG   ILLF+  LP K+ +F
Sbjct:  542 LFYFL--YSMFIIAFGLGFYILLHDDDGSSSLENKE-------YPFFDYLGLTLVKTFTMFVGELEFSDLPIS-----TPVGYLFLISFVFLIVVVMMNLLNGLAVSDTGVIREEAEIHSIVXQVDVISYLEAMLLGDPFNFLSNWPTFVWLRNFPDCNLXSRIY-KVPALRTLFQKITGGPKILLFYDRLPDKRVSF 724          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000012817 (supercontig:LSalAtl2s:LSalAtl2s977:9979:21258:1 gene:EMLSAG00000012817 transcript:EMLSAT00000012817 description:"maker-LSalAtl2s977-augustus-gene-0.7")

HSP 1 Score: 220.705 bits (561), Expect = 4.761e-59
Identity = 177/670 (26.42%), Postives = 319/670 (47.61%), Query Frame = 0

Query:   76 INQPILEEGEKTLLQVAL-QHENVDFS-RALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIA-----------------SDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQVVVKEKPRENTLDYLMEML--ERRDLAYFKAVLRFLEPSEVSTSGVRG-FTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPPIPEEQLEEWD------DEQQSRLEKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLA----YRDWTREMRC-SSCSKCAWSVLGSWPELFIL------SLIILTLWMTIG---ILWLVLSISLVL 703
            +N+   E    T+L +AL Q    +F+ + LL      N+ +  +   PIH A + G  E + +L++     AD N  ++ +G TA+H+A                 +    +  +E LL++   N++ ++N G  +PL+  A++ + E ++LL++ GA     V  +S+++ +    P    S      + ++   + L   L  E      F A     +   +  +   G ++ +Q  C  G    V  L+D G + NY        P+LLAA  G++ +L++FK  N          +        +E ILH I+K+  +      Y   EE       Y K L ++VDD +S FR  ++  IN  D++GN+P+H AAQ   ++TVR+L++  ANIG +N R + P+  I+PE + ++L++    C QG    T E + + F Y FL PP+ +++ E+         E  + + +  LPE + +WY+SQ   HR L+ HPVITSFL +KW+R+R +F  N+ FY  +V   T Y+  +     +        ++   V +           I+   ++++ F   +F++ + +++ L     Y  +T  ++  SS S C  ++L +W E+ +L      +   +T++  I    IL+L  SIS ++
Sbjct:  335 VNKEYSEYNNSTVLHLALSQPGKEEFALQVLLRPDLNPNIPHKIVRSFPIHLAAEIGAHEVIQSLIDI---NADVNARIE-NGNTALHLACLKSNANWIKDPILKQEAQCNYIKCVEALLKKENINIDCENNLG-ISPLYFGAEKGSQEIIQLLIKKGAYAKVEVDDESIEDHLNARFPAGTFSLTPANTQSKDTIENKLFSALYKESTSPGTFIATYNTYKDQTIKWNADNGAYSFLQYCCDLGFKSLVSFLIDEGADPNYYCPNYKMSPILLAAHHGYYKILKIFKELNN-------DIDFSAVDPFKKENILHKIIKRESKA-----YVNFEE-----RNYEKCLSLIVDDEAS-FRSLIQPTINGADHLGNTPLHVAAQLGQEDTVRKLLRCSANIGIKNLRDETPILHISPEVMEEYLDD----CLQGEGLITDERYKIVFSYSFLGPPLIDKKSEKASLLRPEPREMPNMVPQYNLPEAEPLWYMSQLPGHRALLTHPVITSFLCMKWRRMRHYFYINLLFYLLYVGFFTTYLLLKNSEMEIASGTLNTIRIIVMVLL-------VLLTIREFLQAFVSFRRYIFSVENILEISLVSLSFYICFTENLKTESSRSICVAALLLTWAEMVLLFGRHPKASTFVTMFKRISQNFILFLTWSISFII 970          

HSP 2 Score: 191.815 bits (486), Expect = 8.024e-50
Identity = 131/332 (39.46%), Postives = 183/332 (55.12%), Query Frame = 0

Query:  689 TIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTM-FVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGAT----GILLFFSYLPTKQETF-PIRRRSHKHNRGRYDDDE 1014
            T+  + +++ + LVLL +RE  Q   S +RY   +EN +E+ L++L   I F  +        +  +  R +   ALLL WAE++ + GRHP+ +    +VTMF ++ + F  FL W   F+IAF L FYIML +  A+KE        G +   +F +   +L+KT TM   GEIEF  I  +L     P G +  L +VF I++VL+NLLNGLAVSD   IQ+EAEIV+++SR+E ISY ES+LLGDPF F S W P SW R  P  + L  ++ S     RL  K+ G T      LLF   L  KQ  F P R R  K   G +   +
Sbjct:  859 TLNTIRIIVMVLLVLLTIREFLQAFVSFRRYIFSVENILEISLVSLSFYICFTEN--------LKTESSRSICVAALLLTWAEMVLLFGRHPKAS---TFVTMFKRISQNFILFLTWSISFIIAFALAFYIML-KKPATKE--------GEQPNPYFTTVERSLLKTITMSLTGEIEFESIEFDL-----PFGRIVFLLYVFFIMLVLVNLLNGLAVSDISSIQKEAEIVNHVSRIELISYFESMLLGDPFHFFSNWPPFSWGRKVPDCSCLRGVFQS-----RLMSKLIGLTLGSKRFLLFSERLAEKQAIFYPNRSRREKSQMGAHTSSK 1160          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000005636 (supercontig:LSalAtl2s:LSalAtl2s3031:10533:12187:1 gene:EMLSAG00000005636 transcript:EMLSAT00000005636 description:"maker-LSalAtl2s3031-augustus-gene-0.2")

HSP 1 Score: 112.464 bits (280), Expect = 3.574e-26
Identity = 84/245 (34.29%), Postives = 124/245 (50.61%), Query Frame = 0

Query:  693 LWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPD--SALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSD-IPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILL 934
            +W+ L  S  +L  +E FQ+A S K YF   ENW++L +I  V +I F  D   +L   + +    + H  A+ + L W EL+ M+GR P    + IYV MF  V + F  FL  Y   L+AF L F ++     +    G G                 A++KT  M  GEIE+ + I  N +      G+L +L FV L+ ++LMNLL GLAVSD   +Q+ A +   + + E IS+ ES+L 
Sbjct:  181 VWISLLFSTSILMGKECFQLAHSQKMYFYNWENWVQLGIILDVILISFHKDPFDSLEHYIPLIGIWQHHAAAIGVFLVWGELMLMIGRLPT---FGIYVQMFTTVAKNFAKFLAAYFCLLVAFALSFCVLFPNYQSFNVKGRGILS--------------AVIKTLVMMAGEIEYENFIYENGQNLYVFTGHLMVLIFVLLVSIILMNLLVGLAVSDIQGLQKSAGLDRLVRQTELISHIESMLF 408          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000012234 (supercontig:LSalAtl2s:LSalAtl2s88:714578:730796:-1 gene:EMLSAG00000012234 transcript:EMLSAT00000012234 description:"maker-LSalAtl2s88-augustus-gene-6.25")

HSP 1 Score: 91.6633 bits (226), Expect = 7.978e-19
Identity = 71/241 (29.46%), Postives = 123/241 (51.04%), Query Frame = 0

Query:  690 IGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDI--PINLKGPLAPIGY--LFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETI 926
            +G+L     I ++L  L E+ ++  +  RYF   +N ++ W++ ++ + +   D+    + + S   +  + A+ + LAW  L   + + P L    IYV MF+ +L TF  F L +  F+IAFGLGF+  LH                  S++ F +P  + +KT+ M +GE E+ DI  P N   P  P G   +F   FV ++ +++MNLL GLAV D   +Q++A +   + + E +
Sbjct: 2240 VGVL-----IGVILNILAEITEIIRTGIRYF-SFDNLMD-WVVCILSLYIISVDTIFVPEKDCS---RWDVAAILITLAWLNLXAXIXQLPYLG---IYVIMFFDILLTFIKFALIFMVFVIAFGLGFH-FLHT-----------------SHDPFQTPGDSFLKTTVMMIGEYEYVDIFFPGNENDP-KPGGMSKVFFGIFVVVMSLIVMNLLVGLAVEDIKAVQDQAVLKRLVMQAELV 2448          

HSP 2 Score: 59.3066 bits (142), Expect = 6.686e-9
Identity = 116/512 (22.66%), Postives = 204/512 (39.84%), Query Frame = 0

Query:   81 LEEGEKTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSG--------------------------------NLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGAS--LTDLVGTKSVKEAIKENLPYFDVSQVVVKEKPRENTLDYLMEMLERRD--------LAYFKAVLRFLE-----PSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGI--YPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLEL-----VINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLH--------KIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPPIPEEQLEEWDDEQQSRLEKEALP 530
            L+E + + L  A+++ N+D ++ LL  GA  N+   +    P+  A + G                                N+   VALL N +  AD N A      TA+H A+  G  D    LL  S      +D +G  TPL LA+     + VKLLL + A+    D+     + EA +E     +V   ++ E     + D++ E+++++D        +A     ++ +E      + V++      +V+  A   G  + VE+L++     N         PV +AA+  +  V+E     N  C      +N G        T LH+  K+      + +  + T  ++  +D++    +  L + P    +  LE      +I+  D   N P+H A++    E V  L+  G++I ++N      LH        K+  E L    ++ C       D NT    + T +  FL      ++L E+  + QSR  K+  P
Sbjct: 1462 LDEKDVSPLHYAVRYTNIDIAQFLLDMGANPNVQGPD-KMTPLQYAARYGGKKRSRHWKKGSYRSFDEEEDEAEMFECEKVINVGETVALLINYY--ADVN-AQDRYNLTALHHAAIRGNNDAASVLLSTSGIEKEPRDLQG-STPLHLASTYNRLDTVKLLLANKANPKCIDIDERTPLHEACQEG--NVEVVNAIISESEANFSTDFVKELIQKKDGEGNTSLSIAVSSGSIKIVELLLEYGANVNSKNKHSVSVLHCAARTGQIESVELLVEHSAEVNV-VNSLIQTPVYIAASNNNVKVIEYL--VNNGCNPNIQDSN-GF-------TPLHIACKEGNVEAXEVLIAFKTNVDLLDYDDRSCLYIAALYNKPE-VLKALLENDKCNGLISVNDLFDNLPLHVASKEGNVEIVHLLINAGSDIDKKNEDEMTALHLASLNGRVKVVEEILK---SDRCAISDIDEDSNTALHIACT-NRRFLV----SKKLLEYGADIQSRNVKKWTP 1946          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000005243 (supercontig:LSalAtl2s:LSalAtl2s279:373559:377813:-1 gene:EMLSAG00000005243 transcript:EMLSAT00000005243 description:"maker-LSalAtl2s279-snap-gene-3.20")

HSP 1 Score: 76.6406 bits (187), Expect = 2.976e-15
Identity = 68/216 (31.48%), Postives = 102/216 (47.22%), Query Frame = 0

Query:  714 SSLKRY----FLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPIN--------LKGPLAPIGYL---FLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEA 914
            SSL +Y    +   EN +E W   ++  IL            +    +  +GAV + L+W  L++ V + P L    IYV MF  VL+TF  F +    F++AF LGF          + L GG+        E FN+   +++KT  M +GE E+ DI  +        LK    P  YL   F L F+ ++ +++MNLL GLAV D   +Q+ A
Sbjct:   99 SSLTQYARLSYFSFENLVE-WTCYILASILVYDFDECQQITGLRSGWQWQIGAVIVTLSWLNLLSNVRKVPLLG---IYVVMFSDVLKTFLKFSIICGLFIVAFSLGF----------QTLLGGQ--------ENFNTIDKSMMKTIVMMIGEFEYDDIFYDNVRNAGETLKRSSLPYSYLTQGFFLVFLIIMPIIIMNLLVGLAVDDIKAVQDNA 292          

BLAST of transient receptor potential cation channel protein painless vs. L. salmonis genes
Match: EMLSAG00000004066 (supercontig:LSalAtl2s:LSalAtl2s216:451680:461373:-1 gene:EMLSAG00000004066 transcript:EMLSAT00000004066 description:"maker-LSalAtl2s216-snap-gene-4.30")

HSP 1 Score: 72.4034 bits (176), Expect = 5.166e-13
Identity = 104/379 (27.44%), Postives = 169/379 (44.59%), Query Frame = 0

Query:  122 PIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQVVVKEK-PRENT----LDY-LMEMLERRDLAYFKAVLRFLEPSEVSTSGVRG--FTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVW--------TRDTRETILHLILK---KSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPS---SAFRRQLELV--------INKKDNMG--NSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIA 468
            PIH AV S N +  V ++E+ F K            T VHIA+D    D+L+ LL+   A VNA+D+ G QT L  +A+  N +A ++LL HG     +V  K    A    L    V++++ +E  P  +T    ++Y L+E  +  DL   K ++    P  V+   + G   T +  A        VE LL  G +  +   +    P+  A + GHF+V E+  ++      G+      +W        +   +  I+ L+LK      ++N  G  P    I   DE     +++L  D +   +A +  L  V        IN +D+ G  ++P+H AA Y   E    L+Q GA++  ++  G IPLH  +
Sbjct:  367 PIHCAVASPNPKRKV-VVESLFRKGASLNEPNKDHLTPVHIATDKCHYDVLDLLLKHG-AEVNARDDLG-QTALHRSAQEGNVQACRILLSHGVDAI-MVSYKGFTAA---QLATEAVAKLLAEEPGPPMSTAGADIEYQLLEAAKAGDLELVKKIV-LEHPHIVNCRDLDGRHSTPLHFASGYNRVGVVEFLLSNGADV-HAKDKGGLVPLHNACSYGHFEVTELLVKH------GASVNVSDLWKFTPLHEASAKGKYEIVKLLLKYGADPTKKNRDGHTP----IDLVDEGDQDVMDLLKGDSALLDAAKKGNLTRVQKLLTTDNINCRDSQGRNSTPLHLAAGYNNLEVAYFLLQSGADVNAQDKGGLIPLHNAS 726          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|657193258|sp|Q7Z020.4|TRPA1_DROME (RecName: Full=Transient receptor potential cation channel subfamily A member 1; Short=dTRPA1; AltName: Full=Ankyrin-like with transmembrane domains protein 1; Short=dANKTM1)

HSP 1 Score: 128.642 bits (322), Expect = 9.110e-29
Identity = 185/850 (21.76%), Postives = 343/850 (40.35%), Query Frame = 0

Query:  122 PIHNAVQSGNLEHVVALLE-NPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQVVVKEKPRENTLDYLMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCM-----KGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGA----NIGQRNWRGDIPLHKIAPETLADFLNED--------------CVT------------------CPQGHDPNTHEDFSVTFHYDFLAPPIPEEQLEEWDDEQQSRLEKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFN-RNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMRCSSCSKCAWSVLGSWPELFILSLIILTLWMTIGILW--LVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMIL----FIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPIN------LKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEI 916
            P+H A   G ++ V  + E  P  K            T +H AS     DI+  L+ E  A++NA D K H++PL LAA R   + V LL+  GA +       SVK+A   N+ +F     V+    R      L +  E+      +A L+ L    ++     G + +  A  +G    +E L+  G   N     N   P+  AA  G ++ +       +         G+  T + + ++     ++ L+L +    +       P +++     YT+++E+L    S         ++++ D  GN+ +H A        +  LM +G     N+   +       +K     LA   +E+              CVT                    + +     + F + + + FL  P    +++E   E  +      LP  +T+     +     L+ HP+   +L +KW    ++F+  N+  Y+ F+V +T  I++    ++++L+   N  ++            +C+         +G+  +   L     V         ++R  SC +                     +  T  IL+  +V+ + ++L  +REL Q+      Y L   N I  W++ +  +++    F PD  +++       I     ++A+ L+W  L+  + R  ++    IYV MF ++L+T    L+ +S  +IAFGL FYI+L +            D       F N P ++L++T +M +GE++F    +N      LK P+    +L L  F+ L+ ++LMNLL GLAV D   ++  A++
Sbjct:  302 PVHLACAQGAIDIVKLMFEMQPMEKRLCLSCTDVQKMTPLHCASMFDHPDIVSYLVAEG-ADINALD-KEHRSPLLLAASRSGWKTVHLLIRLGACI-------SVKDAAARNVLHF-----VIMNGGR------LTDFAEQVANCQTQAQLKLL----LNEKDSMGCSPLHYASRDGHIRSLENLIRLGACINLKNNNNE-SPLHFAARYGRYNTVRQLLDSEKGSFIINESDGAGMTPLHISSQQGHTRVVQLLLNRGALLHRDHTGRNPLQLAAM-SGYTETIELLHSVHSH--------LLDQVDKDGNTALHLATMENKPHAISVLMSMGCKLVYNVLDMSAIDYAIYYKYPEAALAMVTHEERANEVMALRSDKHPCVTLALIASMPKVFEAVQDKCITKANCKKDSKSFYIKYSFAFLQCPFMFAKIDEKTGESITTASPIPLPALNTMV----THGRVELLAHPLSQKYLQMKWNSYGKYFHLANLLIYSIFLVFVT--IYSSLMMNNIELKAGDNKTMSQ-----------YCN---------MGWEQLTMNLSQNPSV-------ASQIRLDSCEE--------------------RINRTTAILFCAVVIVVYILLNSMRELIQIYQQKLHYILETVNLIS-WVLYISALVMVTPAFQPDGGINT-------IHYSAASIAVFLSWFRLLLFLQRFDQVG---IYVVMFLEILQTLIKVLMVFSILIIAFGLAFYILLSKII----------DPQPNHLSFSNIP-MSLLRTFSMMLGELDFVGTYVNTYYRDQLKVPMT--SFLILSVFMILMPILLMNLLIGLAVGDIESVRRNAQL 1040          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|62900887|sp|Q9W0T5.2|PYX_DROME (RecName: Full=Transient receptor potential channel pyrexia)

HSP 1 Score: 124.79 bits (312), Expect = 1.138e-27
Identity = 84/254 (33.07%), Postives = 127/254 (50.00%), Query Frame = 0

Query:  707 RELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPI-GYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLL 959
            +E+FQMA  L+ Y    ENW++ W I    ++   P++  + DL      + H+ A+ +LL W EL+ +VGR P    + +YV MF KV   F  FLL Y   L+AFGL F ++ +   A                 F N  W + +K+ TM  GE+EF DI         P+  ++  L+FV L+ V+L NL+ GLAVSD   +Q  A +   + + E +S  ES+     F  L + AP +  ++C    LL
Sbjct:  548 KEVFQMAHGLRGYAKYWENWLQ-WTIGTGVLLCVTPETVRTDDLTAVPVWQHHVAAIVILLVWLELMMLVGRFPI---FGVYVQMFTKVAVNFAKFLLAYICLLVAFGLSFAVLFNDYPA-----------------FENITW-SFLKSITMMSGELEFEDIFYGDYAVKFPVTAHIIFLSFVLLVTVILTNLMVGLAVSDIQGLQVSATLDRLVRQAELVSRLESLF----FSRLLRSAPTNLIQLCKRSALL 775          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|56749363|sp|Q9W0Y6.1|PAIN_DROME (RecName: Full=Transient receptor potential cation channel protein painless)

HSP 1 Score: 104.76 bits (260), Expect = 1.542e-21
Identity = 81/253 (32.02%), Postives = 117/253 (46.25%), Query Frame = 0

Query:  694 WLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCD--IKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQD---------TASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLG 935
            WL +S     L LRE  Q   S  RYF  + N +E+ LI L          ++ + +E S D   +R +    +LL   E   +VG  P L+  + ++ M  +V  +F      YS F++ F L FYI+  +          T    LG  EG D  + +  F  P  A++KT  M  GE +   I        +   YL  L FV  + +VL NLLNGLAVSDT +I+ +AE+   I R   +S  E +L G
Sbjct:  534 WLGIS----YLILRECIQWIMSPVRYFWSITNIMEVALITL----------SIFTCMESSFDKETQRVLAVFTILLVSMEFCLLVGSLPVLS-ISTHMLMLREVSNSFLKSFTLYSIFVLTFSLCFYILFGKSVEEDQSKSATPCPPLGKKEGKDEEQGFNTFTKPIEAVIKTIVMLTGEFDAGSIQFT-----SIYTYLIFLLFVIFMTIVLFNLLNGLAVSDTQVIKAQAELNGAICRTNVLSRYEQVLTG 766          

HSP 2 Score: 98.9821 bits (245), Expect = 9.282e-20
Identity = 147/691 (21.27%), Postives = 288/691 (41.68%), Query Frame = 0

Query:   50 LDDTIFLRGSLSDDEKKKLLLPLDHWINQPILEEGEKTLLQVALQHENV-DFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLE-NPFNKADPNMA---MQPSGQTAVHIASDLGKLDILE--RLLQESKANVNAKDNKGHQTPLFLAAKRRNAEA-----VKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQ-----VVVKEKPRENTLDYLMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAG--VNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPPIPEEQLEEWDDEQQSRLEKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLA----YRDWTREMRCSSCSKCAWSVLGSWPE--------LFILSLIILTLWMTIGILWLVLSISLVLLGLREL 709
            +D    L G+L+  + ++ +  LD      + ++   ++ + AL      DF  A +  G+  N  N +LD+A I  A  S +  ++ ALL+  P NK   +     + P    A ++ +D    D+    +LL +  A+ N  D +G  TPL    ++   +A     ++L L+H     D      V+  ++   P   + +       +  +  + TL    E L  +  A +   L+    ++++      F ++Q++   G     +V+L  G  +NS  G + N    V  A   G++  LE  +   +  ++ +P + +                       L  +    +E       + +  E+L++           + IN+ D+    P+ +A +Y     +++L++ GA IG ++  G +P+  + PE L +   + C+T   G  P   ++F +   Y  L        + +  D   ++L+ E  P    I ++++SK  RHL++HP+I+SFL+LKW R+   F  N   Y+ F  ++  Y   +F                HE   ++     F   + W     LG  Y++  L  C+Q +++    +   T  M  +  +   ++ + S  +        +F + L+ +   + +G L  VLSIS  +L LRE+
Sbjct:    9 IDPQAQLAGALAKQDIRQFVAALDSGALADLQDDRHTSIYEKALSTPGCRDFIEACIDHGSQVNYINKKLDKAAISYAADSRDPGNLAALLKYRPGNKVQVDRKYGQLTPLNSLAKNL-TDENAPDVYSCMQLLLDYGASPNIVD-QGEFTPLHHVLRKSKVKAGKKELIQLFLDHPELDIDSYRNGEVRRLLQAQFPELKLPEERHTGPEIDIQTLQRTLRDGDETLFEQQFAEYLQNLKGGADNQLNAHQEEYFGLLQESIKRGRQRAFDVILSTGMDINSRPG-RANEANLVETAVIYGNWQALE--RLLKEPNLRLTPDSKL-----------------------LNAVIGRLDEPPYDGSSHQRCFELLINSD--------RVDINEADSGRLVPLFFAVKYRNTSAMQKLLKNGAYIGSKSAFGTLPIKDMPPEVLEEHF-DSCITT-NGERPG-DQNFEIIIDYKNL--------MRQERDSGLNQLQDEMAP----IAFIAESKEMRHLLQHPLISSFLFLKWHRLSVIFYLNFLIYSLFTASIITYTLLKF----------------HES--DQRALTAFFGLLSW-----LGISYLI--LRECIQWIMSPVRYFWSITNIMEVALITLSIFTCMESSFDKETQRVLAVFTILLVSMEFCLLVGSLP-VLSISTHMLMLREV 622          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|205831270|sp|Q18297.5|TRPA1_CAEEL (RecName: Full=Transient receptor potential cation channel subfamily A member 1 homolog)

HSP 1 Score: 88.1965 bits (217), Expect = 2.782e-16
Identity = 67/254 (26.38%), Postives = 120/254 (47.24%), Query Frame = 0

Query:  692 ILWLVLSISLVLLG-LRELFQMASSLKRYFLCLENWIE--LWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDI--------PINLKGPL-APIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESIL 933
            ++W ++  +L +   L E FQ+      Y +  ENWI+  ++  AL+ +  F   SA S    +  + +  + A+ +   W  L+ M+ + PR   + I+V MF  +++TFF F   +  F+IAF   FY++L                       F++ +++ +KT+ M +GE EF+ I           + GP    +       F  ++ ++LMNLL GLAV D   +QE+AE+     +V+ +   E+ L
Sbjct:  869 VVWKIIIQTLAVCQILVECFQLFQRKFAYLVNWENWIDCFIYSTALITVYDFSECSATSG---VRQNWQWILAALCIFFGWINLLFMIRKMPR---FGIFVVMFVDIVKTFFRFFPVFVLFIIAFSSSFYVILQNRPE------------------FSTIFMSPLKTTVMMIGEFEFTGIFHGDETTHAEKMFGPAHTAVACALFFFFCIIMTILLMNLLVGLAVDDIKGVQEKAELKRLAMQVDLVLQIEASL 1098          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|313104269|sp|O75762.3|TRPA1_HUMAN (RecName: Full=Transient receptor potential cation channel subfamily A member 1; AltName: Full=Ankyrin-like with transmembrane domains protein 1; AltName: Full=Transformation-sensitive protein p120)

HSP 1 Score: 87.8113 bits (216), Expect = 3.401e-16
Identity = 72/265 (27.17%), Postives = 128/265 (48.30%), Query Frame = 0

Query:  707 RELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLH-QDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSD--IPINLKGPLA-PI-GYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFL---SQWAPVSWARMCPSGTLLYHLY 963
            +E  Q+    + YF+ + N +E W+I   G+I  +P       +E+   ++   GA+A+   W   +  +    R     I++ M   +L+T     + + F L+AFGL FYI+L+ QD                    F+SP L++++T +M +G+I + +  +   L+  LA P+  +  L++F   + +VLMNLL GLAV D   +Q+ A +     +VE  +  E  L   P  FL    Q + + +     SG +L+H++
Sbjct:  787 KEAGQIFQQKRNYFMDISNVLE-WIIYTTGIIFVLP-----LFVEIPAHLQWQCGAIAVYFYWMNFLLYL---QRFENCGIFIVMLEVILKTLLRSTVVFIFLLLAFGLSFYILLNLQDP-------------------FSSPLLSIIQTFSMMLGDINYRESFLEPYLRNELAHPVLSFAQLVSFTIFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTSLEKKL---PLWFLRKVDQKSTIVYPNKPRSGGMLFHIF 1020          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|56749669|sp|Q6RI86.1|TRPA1_RAT (RecName: Full=Transient receptor potential cation channel subfamily A member 1; AltName: Full=Ankyrin-like with transmembrane domains protein 1)

HSP 1 Score: 83.1889 bits (204), Expect = 9.229e-15
Identity = 71/264 (26.89%), Postives = 120/264 (45.45%), Query Frame = 0

Query:  690 IGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHM----GAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDI---PI---NLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQ 943
            + I  +++ +S +    +E+ Q+    + YFL   N +E W+I    MI  +P         +  DI  +M    GA+A+   W   +  +    R     I++ M   + +T       + F L+AFGL FY++L+   A                  F++P L+L++T +M +G+I + D    P+    L  P+   G L  +AF   + +VLMNLL GLAV D   +Q+ A +     +VE  +  E  L   PF +L +
Sbjct:  773 LKICMILVFLSSIFGYCKEVVQIFQQKRNYFLDYNNALE-WVIYTTSMIFVLP---------LFLDIPAYMQWQCGAIAIFFYWMNFLLYL---QRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDA------------------FSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQL--IAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVELHTNLEKKL---PFWYLRK 1000          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|56749781|sp|Q8BLA8.1|TRPA1_MOUSE (RecName: Full=Transient receptor potential cation channel subfamily A member 1; AltName: Full=Ankyrin-like with transmembrane domains protein 1)

HSP 1 Score: 81.2629 bits (199), Expect = 3.218e-14
Identity = 64/241 (26.56%), Postives = 111/241 (46.06%), Query Frame = 0

Query:  690 IGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDI---PI---NLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVE 924
            I I  +++ +S +    +E+ Q+    + YFL   N +E W+I    +I  +P       L +   ++   GA+A+   W   +  +    R     I++ M   + +T       + F L+AFGL FY++L+   A                  F++P L+L++T +M +G+I + D    P+    L  P+   G L  +AF   + +VLMNLL GLAV D   +Q+ A +     +VE
Sbjct:  773 IKICMILVFLSSIFGYCKEVIQIFQQKRNYFLDYNNALE-WVIYTTSIIFVLP-----LFLNIPAYMQWQCGAIAIFFYWMNFLLYL---QRFENCGIFIVMLEVIFKTLLRSTGVFIFLLLAFGLSFYVLLNFQDA------------------FSTPLLSLIQTFSMMLGDINYRDAFLEPLFRNELAYPVLTFGQL--IAFTMFVPIVLMNLLIGLAVGDIAEVQKHASLKRIAMQVE 984          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|332278249|sp|O15084.5|ANR28_HUMAN (RecName: Full=Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A; Short=PP6-ARS-A; Short=Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A; AltName: Full=Ankyrin repeat domain-containing protein 28; AltName: Full=Phosphatase interactor targeting protein hnRNP K; Short=PITK)

HSP 1 Score: 75.8702 bits (185), Expect = 1.507e-12
Identity = 115/426 (27.00%), Postives = 183/426 (42.96%), Query Frame = 0

Query:  119 DQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTD-----------LVGTKSVK--EAIKENLPYFDVSQVVVKEKPRENTLDYLMEML----------------ERRDL---AYFK--AVLRFL--EPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSW--RPVLLAAARGH----FDVLEVFKRYNQKCMKG-SPQTNVGVWTRDTRETILHLILKKSFRRNLQGI-YPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVT--CPQGHDPNTHEDFSVT 498
            DQ  +  A+ +G+ + V AL+   F K D N       +T +H A+ LG  +I+E LL  S A VNAKD+K   TPL  A    + EAV++LL+H A +                 K+VK  EA+   L   +VS    +            EM+                +RR +   AY     V++ L    +EV+    + +T +  A   G+   V+ LLD GV+ N   + N++   P+ +A   G      ++++     NQK  KG +P       T       L L++      N++     TP  ++    ++++S  I+          Q   VI+ +D  GN+P+H AA+Y  +  +  L+  GA+  +R   G  PLH  A   L+ F   DC       G D +T +DF  T
Sbjct:    8 DQPSLVQAIFNGDPDEVRALI---FKKEDVNF-QDNEKRTPLHAAAYLGDAEIIE-LLILSGARVNAKDSKW-LTPLHRAVASCSEEAVQVLLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMN---EPNAYGNTPLHVACYNGQDVVVNELIDCGAIVNQKNEKGFTPLHFAAASTHGA--LCLELLVGNGADVNMKSKDGKTPLHMTALHGRFSRSQTII----------QSGAVIDCEDKNGNTPLHIAARYGHELLINTLITSGADTAKRGIHGMFPLHLAA---LSGF--SDCCRKLLSSGFDIDTPDDFGRT 407          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|81908906|sp|Q505D1.1|ANR28_MOUSE (RecName: Full=Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A; Short=PP6-ARS-A; Short=Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A; AltName: Full=Ankyrin repeat domain-containing protein 28; AltName: Full=Phosphatase interactor targeting protein hnRNP K; Short=PITK)

HSP 1 Score: 75.485 bits (184), Expect = 1.640e-12
Identity = 111/424 (26.18%), Postives = 181/424 (42.69%), Query Frame = 0

Query:  119 DQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTD-----------LVGTKSVK--EAIKENLPYFDVSQVVVKEKPRENTLDYLMEML----------------ERRDL---AYFK--AVLRFL--EPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSW--RPVLLAAARGH----FDVLEVFKRYNQKCMKG-SPQTNVGVWTRDTRETILHLILKKSFRRNLQGI-YPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVT 498
            DQ  +  A+ +G+ + V AL+   F K D N       +T +H A+ LG  +I+E LL  S A VNAKD+K   TPL  A    + EAV++LL+H A +                 K+VK  E++   L   +VS    +            EM+                +RR +   AY     V++ L    +EV+    + +T +  A   G+   V+ LLD GV+ N   + N++   P+ +A   G      ++++     NQK  KG +P       T       L L++      N++     TP  ++    ++++S  I+          Q   VI+ +D  GN+P+H AA+Y  +  +  L+  GA+  +R   G  PLH  A    +D   +       G D +T +DF  T
Sbjct:    8 DQPSLVQAIFNGDPDEVRALI---FKKEDVNF-QDNEKRTPLHAAAYLGDAEIIE-LLILSGARVNAKDSKW-LTPLHRAVASCSEEAVQILLKHSADVNARDKNWQTPLHIAAANKAVKCAESLVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMN---EPNAYGNTPLHVACYNGQDVVVNELIDCGANVNQKNEKGFTPLHFAAASTHGA--LCLELLVGNGADVNMKSKDGKTPLHMTALHGRFSRSQTII----------QSGAVIDCEDKNGNTPLHIAARYGHELLINTLITSGADTAKRGIHGMFPLHLAALSGFSDCCRK---LLSSGFDIDTPDDFGRT 407          

BLAST of transient receptor potential cation channel protein painless vs. SwissProt
Match: gi|116241246|sp|P16157.3|ANK1_HUMAN (RecName: Full=Ankyrin-1; Short=ANK-1; AltName: Full=Ankyrin-R; AltName: Full=Erythrocyte ankyrin)

HSP 1 Score: 71.633 bits (174), Expect = 3.120e-11
Identity = 110/433 (25.40%), Postives = 186/433 (42.96%), Query Frame = 0

Query:   74 HWINQPILEEGEK---------TLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGAS--LTDLVGTKSVKEAIKEN-------LPYFDVSQVVVKEK---PRENTLDY----LMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQ--TNVGVWTRDTRETILHLILKKSFRRNL---QGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFL 476
            H + + +L++G K         T L +A +  +V     LL  GA  +    E    P+H A   G+L  V  LL+     A PN++     +T +H+A+  G  ++ + LLQ +KA VNAK  K  QTPL  AA+  +   VKLLLE+ A+  L    G   +  A +E        L   + SQ  + +K   P      Y    + E+L  RD             +  + +G  G T +  A      D V++LL  G  S +    N + P+ +AA +   +V     +Y       S Q  T + +  ++    ++ L+L K    NL    G+  TP  +    E +    ++L+         +  ++++    MG +P+H A+ Y   + V+ L+Q  A++  +   G  PLH+ A +   D +
Sbjct:  351 HRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVT-ESGLTPLHVASFMGHLPIVKNLLQR---GASPNVS-NVKVETPLHMAARAGHTEVAKYLLQ-NKAKVNAK-AKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERD-------------AHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRG-GSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGL--TPLHLV-AQEGHVPVADVLI---------KHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQGHTDIV 750          

HSP 2 Score: 59.3066 bits (142), Expect = 1.805e-7
Identity = 92/346 (26.59%), Postives = 146/346 (42.20%), Query Frame = 0

Query:  123 IHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQVVVKEKPRENTLDYLMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIA 468
            +H A + G+++ VV LL    +K          G TA+HIA+  G+ +++  L+    ANVNA+  KG  TPL++AA+  + E VK LLE+GA+            A+     + +V   ++    +       + +  R D     AVL   +P+    S   GFT +  A      +  ++LL+ G + N+ T +N   P+ +A+ RG+  ++ +         +G+    +   T+D   T LH   +    R           IS          EIL+D  +          I  K   G SPIH AAQ    + VR L+Q  A I         PLH  A
Sbjct:   49 LHLASKEGHVKMVVELL----HKEIILETTTKKGNTALHIAALAGQDEVVRELVNYG-ANVNAQSQKGF-TPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKT-GFTPLHIAAHYENLNVAQLLLNRGASVNF-TPQNGITPLHIASRRGNVIMVRLL------LDRGA---QIETKTKDEL-TPLHCAARNGHVR-----------IS----------EILLDHGAP---------IQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAA 346          

HSP 3 Score: 58.5362 bits (140), Expect = 3.234e-7
Identity = 60/206 (29.13%), Postives = 92/206 (44.66%), Query Frame = 0

Query:   67 KLLLPLDHWINQPILEEGEKTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNM-----------------------------AMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQVV 243
            KLLLP     + P       T L +A +   V+ +R+LL  G  AN  + +    P+H A Q G+ E V  LL    N    N                              A    G T +H+AS  G + +++ LLQ  +A+VNAK   G+ +PL  AA++ + + V LLL++GAS  ++    +   AI + L Y  V+ V+
Sbjct:  586 KLLLPRGGSPHSPAW--NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQ-GVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQH-QADVNAKTKLGY-SPLHQAAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVL 786          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325325088|ref|XP_023344392.1| (transient receptor potential cation channel protein painless-like [Eurytemora affinis])

HSP 1 Score: 922.154 bits (2382), Expect = 0.000e+0
Identity = 502/1082 (46.40%), Postives = 690/1082 (63.77%), Query Frame = 0

Query:   15 VCAQLSCQDAQSGVLDALRRDDPTTFSSILAEECHLDDTIFLRGSLSDDEKKKLLLPLDHWINQPILEE----GEKTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLE-HVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQVVVKEKPRENTLDYLMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQ------KCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPPIPEEQLEEWDDEQQSRLEKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQ-NFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMRCSSCSKCAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGILLFFSYLPTKQETFPIRRRSHKHNRGRYDDDEEVEEEDCCPCCHQDDGNYEDVLKSARAILLQRTNPPAQAGDTALMMKIMDKQDTLMKLVELQHEEMRHLR 1084
            VCAQLSC D Q  + + +   D TTFS ILA+   LDD   L  SLSDDEKK   LP +HWINQP++ +     ++ LLQV++Q   V+F++ L+  GA AN+YNDE +QA IH AV S + E H+ ALL +  NKAD N  +QPS  T +H+A++ GKL  L  LL+     ++ +D  G +TPLFLAA+ ++ E V+ L+E GASL    G  + +  I+ENLPY D+S++ VKE+P  NT++ L + +ER+D   F+  L  +   +VS   VRG+T +QKA +  L  +V  LL+ GVN N  + EN  RPVLLA+ RGH+ VL VF    +      K  K SP  N  VW+RDT+ET LH+ILKKS  + L G+  +  E+   DE Y K L IL+   S   R QL  VINK+D +  +P+HYAAQ W+Q  + +L++LGANIG RN RG+IPL +I PETL +FLN     C    +   H+DF V F+Y +LAPP+ + Q EEWD  +Q  LE+  LPET+++W +SQSK+HRHL++HP +TSFLWLKWQR+RRFF+RN+R Y  FV  LTWYIF RFGG  L       +        +  S    FC   ++   + LGFW V+F + S   ++ A RD  RE  C S +    + L SW E+F+ +LIIL      G LW V+++  V L LRE+FQM +SLKRYF  LEN +E+ ++ L+G++LF PD+ L    +  C + RH+ AV +LL+W E+I +V +HPRL+RYN+Y++MFYKVL+TFF FL+WYSFF+IAF LGFYIMLH+D  +            + Y +F+SPW +LVKTSTMFVGE+EFSDIPI+ +  LA + + FLL FVF IVV+LMNLLNGLAVSDTG+I E+AEIVSY +RVETISY ES+LLGDPF FLS W  VS     PS  L   +Y++CP  +++ H +TGATGILLF+S LP K+ +FP+                  E+++ C  C       +D+L++AR + L++     +  D  +  KI       +K++E+Q E+++ L+
Sbjct:   23 VCAQLSCGDPQENLSNCILSGDLTTFSCILADIAPLDDHHQL--SLSDDEKKVYPLPEEHWINQPLISDNPNLAQQLLLQVSVQQGAVEFTQLLVRAGARANMYNDETNQAVIHTAVLSQHGEDHLTALLSDEKNKADINCTLQPSSLTPLHLAAERGKLTCLNLLLEHPDIQIDPRDGSGGRTPLFLAARGKHVECVRKLVEQGASLDIKCGRTTPRTVIQENLPYLDLSKIKVKERPVANTVETLFQYIERKDFLRFRYTLTKVSLRDVSEKSVRGYTCLQKAVLANLPTFVTQLLNHGVNPNLYSVENPVRPVLLASYRGHYQVLSVFVEELETALSLGKVSKVSP--NFAVWSRDTQETCLHMILKKSHSKALLGL-GSDSELKGLDEDYRKCLNILLS--SEQVRSQLGRVINKRDILSYTPLHYAAQMWSQADITKLLKLGANIGVRNSRGEIPLSRILPETLEEFLNH----CSNHENHPLHQDFKVEFNYSWLAPPVDDYQSEEWDQHRQDELERAGLPETESLWCMSQSKSHRHLLRHPTVTSFLWLKWQRVRRFFSRNIRIYLLFVTCLTWYIFTRFGGIKLNSSEPSLDTGALDPPAVSSSNSTQFCKHPRFDSGAQLGFWMVLFVIESIFLLIYAIRDLFRECGCKSGTDFMIAFLSSWFEMFLAALIILIFITGEGGLWWVITLLFVTLTLREIFQMTASLKRYFFSLENLLEIAMLGLLGVLLFAPDT-LDPTEDGDCVVLRHIAAVCILLSWTEMIILVAKHPRLSRYNVYISMFYKVLQTFFLFLVWYSFFIIAFALGFYIMLHKDIPN-------FTPDSDHYVYFDSPWTSLVKTSTMFVGELEFSDIPIDTESNLAWLSFTFLLVFVFFIVVILMNLLNGLAVSDTGIIMEKAEIVSYTARVETISYMESVLLGDPFLFLSHWPRVSCLARLPSLALCNQVYSACPGARKVGHCLTGATGILLFYSLLPEKKASFPM------------------EDKEGCGGCRVVQDIPQDILQAARDLALKKETEKEEEADQKINSKI-------LKMLEIQQEQIQSLQ 1060          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325320375|ref|XP_023341985.1| (transient receptor potential channel pyrexia-like isoform X2 [Eurytemora affinis])

HSP 1 Score: 791.186 bits (2042), Expect = 0.000e+0
Identity = 445/1107 (40.20%), Postives = 661/1107 (59.71%), Query Frame = 0

Query:    2 ERVPLMTMNTSNKVCAQL------SC-QDAQSGVLDALRRDDPTTFSSILAEECHLDDTIFLRGSLSDDEKKKLLLPLDHWINQPILEEGE-KTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKA--NVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQVVVKEKPRENTLDYLMEMLER----------------RDLAYFKAVLRFL---EPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFR--RNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPPIPEEQLEEWDDEQQSRLEKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQL--RGDQNFKVAHEVG--IEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMRCSSCSKCAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGILLFFSYLPTKQETFPIRRRSHKHNRGRYDDDEEVEEEDCCPCCHQDDGNYEDVLKSARAILLQRTN----PPAQAGDTALMMKIMDKQDTL 1069
            E +PL ++    K   +       SC  D Q+ VL+ +   D    +S+LAE    +D +        +  +   LP DHWINQP  + G  +TLL +A++  ++     LL  GA A+L+N++L  +P+H A Q  +L+ +  L+ +P N  + N  M+  G+T +HIA+D  +L+IL+ +L E+KA   V+ +D +G QTPL+L+ K++N EAV+LLLE+GA L + V  K+++E +KEN+P F+   V +K K +E   + +   L R                ++L  FK  ++ L   +   + +    GFT +QK+C  G  ++V +LL+ G + N   + NS +PVLLAA  GH  VL+V  ++    ++G    N+ V  + T+ET+LH +L K  R  R   G YP               L +L++    +   +L  ++N  D  GN+ +HYA Q WTQETVR+L++ GANIG +N   + P+ KI PET+  FL+E C+T     +   HEDF + F Y FLAPP+     +E D E +  +E  ALPET+++WY++ S+ HRHL+KHPVITSFLW+KWQRIR++FNR +R Y  FV  L+W+IF  +GG S ++  + D N          +EE      CS +        GFWYVVF L S +Q+ +  RDW R++R S        +  SW E FILS I++ + ++   L  +L+I ++++ LRE+ QM  SLKRY   +EN  EL ++ALV + L++P+  L +     C +KRH+   AL++AWA LIT+VGRHP+L+RYNIYVTMFYKVL++F  FL WYSFF+IAFGLGFYIMLH+D       G    +G + Y+FF  PWL+LVKT TMF+GEIEFS+IPI+L   +  +GY+FL+ F+FLIVVVLMNLLNGLAVSDTG+I+EEAEIV+YISRVETIS  E++LLGDPF+FL+ W  +++ R  PS      LY +   ++ +S+KITGATGILLF+++LP K+      RR+++                    C   D    +++++A+ I++++T      P +  D  L++ +   Q+ L
Sbjct:    8 EGIPLYSLQQEKKNTKKSEFIRVGSCISDPQTNVLECVLHQDHDGLTSVLAEVAPDEDGV--------EASQNYPLPPDHWINQPTSKLGNFRTLLHIAIEQIDLKSVCLLLRGGARADLHNEDLGVSPVHVACQVNSLDILAELVRDPRNSGNINAGMR-DGRTCLHIAADKNRLEILQFIL-ENKALQTVDPEDLRGRQTPLYLSVKKKNIEAVRLLLENGAQLLNTVDNKTIREHMKENIPNFNPESVQIKIKIKERNGEDVAYSLNRILDKAQMNIIKNLPNSQELIQFKTFIQSLIAMDKKLLDSYDCGGFTFLQKSCEYGFSEFVHILLENGAHPNQTMETNSTKPVLLAAYGGHQGVLQVLLQHKIDSLEGGQVVNLTVQEK-TKETVLHYVLSKPSRGPRIQGGDYP-------------GCLNLLLECKDRSVVDELNRIMNWSDRQGNTALHYATQLWTQETVRKLLERGANIGVKNRWSETPISKILPETMESFLDEFCLT---STNDVQHEDFELKFRYSFLAPPVDSPNFDEHDPEGREMVENRALPETESLWYMADSQYHRHLLKHPVITSFLWMKWQRIRKYFNRGLRVYLLFVTCLSWFIFEAYGGVSYRVNSKTDTNSSSPESTESTVEEKEQRSLCS-VNLEPTESRGFWYVVFILQSIIQIFMILRDWYRDLRQSDLKTAFQVLFTSWIEYFILSSIVVLIILSTQALVYILTILILMVALREVLQMMVSLKRYIFDIENLFELVMLALVCICLYVPEHYLYN----GCLLKRHLAGFALVIAWATLITLVGRHPKLSRYNIYVTMFYKVLKSFILFLTWYSFFIIAFGLGFYIMLHRDVP-----GYTPKEGEDEYKFFEDPWLSLVKTGTMFIGEIEFSNIPIDLTTKVGILGYIFLICFIFLIVVVLMNLLNGLAVSDTGIIREEAEIVTYISRVETISCTEAVLLGDPFNFLANWPAIAFLRDVPSMAFCRSLYRNS-LVRNISNKITGATGILLFYTWLPDKELVLQPNRRTNRF------------------VCMPTDCMGAEIIQAAKDIVIKKTRLGLEGPGEISD--LLLNLQKNQENL 1056          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325320373|ref|XP_023341984.1| (transient receptor potential cation channel protein painless-like isoform X1 [Eurytemora affinis])

HSP 1 Score: 787.334 bits (2032), Expect = 0.000e+0
Identity = 447/1116 (40.05%), Postives = 662/1116 (59.32%), Query Frame = 0

Query:    2 ERVPLMTMNTSNKVCAQL------SC-QDAQSGVLDALRRDDPTTFSSILAEECHLDDTIFLRGSLSDDEKKKLLLPLDHWINQPILEEGE-KTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKA--NVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQVVVKEKPRENTLDYLMEMLER----------------RDLAYFKAVLRFL---EPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFR--RNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPPIPEEQLEEWDDEQQSRLEKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQL--RGDQNFKVAHEVG--IEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMRCSSCSKCAWSVL---------GSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGILLFFSYLPTKQETFPIRRRSHKHNRGRYDDDEEVEEEDCCPCCHQDDGNYEDVLKSARAILLQRTN----PPAQAGDTALMMKIMDKQDTL 1069
            E +PL ++    K   +       SC  D Q+ VL+ +   D    +S+LAE    +D +        +  +   LP DHWINQP  + G  +TLL +A++  ++     LL  GA A+L+N++L  +P+H A Q  +L+ +  L+ +P N  + N  M+  G+T +HIA+D  +L+IL+ +L E+KA   V+ +D +G QTPL+L+ K++N EAV+LLLE+GA L + V  K+++E +KEN+P F+   V +K K +E   + +   L R                ++L  FK  ++ L   +   + +    GFT +QK+C  G  ++V +LL+ G + N   + NS +PVLLAA  GH  VL+V  ++    ++G    N+ V  + T+ET+LH +L K  R  R   G YP               L +L++    +   +L  ++N  D  GN+ +HYA Q WTQETVR+L++ GANIG +N   + P+ KI PET+  FL+E C+T     +   HEDF + F Y FLAPP+     +E D E +  +E  ALPET+++WY++ S+ HRHL+KHPVITSFLW+KWQRIR++FNR +R Y  FV  L+W+IF  +GG S ++  + D N          +EE      CS +        GFWYVVF L S +Q+ +  RDW R++R S        VL          SW E FILS I++ + ++   L  +L+I ++++ LRE+ QM  SLKRY   +EN  EL ++ALV + L++P+  L +     C +KRH+   AL++AWA LIT+VGRHP+L+RYNIYVTMFYKVL++F  FL WYSFF+IAFGLGFYIMLH+D       G    +G + Y+FF  PWL+LVKT TMF+GEIEFS+IPI+L   +  +GY+FL+ F+FLIVVVLMNLLNGLAVSDTG+I+EEAEIV+YISRVETIS  E++LLGDPF+FL+ W  +++ R  PS      LY +   ++ +S+KITGATGILLF+++LP K+      RR+++                    C   D    +++++A+ I++++T      P +  D  L++ +   Q+ L
Sbjct:    8 EGIPLYSLQQEKKNTKKSEFIRVGSCISDPQTNVLECVLHQDHDGLTSVLAEVAPDEDGV--------EASQNYPLPPDHWINQPTSKLGNFRTLLHIAIEQIDLKSVCLLLRGGARADLHNEDLGVSPVHVACQVNSLDILAELVRDPRNSGNINAGMR-DGRTCLHIAADKNRLEILQFIL-ENKALQTVDPEDLRGRQTPLYLSVKKKNIEAVRLLLENGAQLLNTVDNKTIREHMKENIPNFNPESVQIKIKIKERNGEDVAYSLNRILDKAQMNIIKNLPNSQELIQFKTFIQSLIAMDKKLLDSYDCGGFTFLQKSCEYGFSEFVHILLENGAHPNQTMETNSTKPVLLAAYGGHQGVLQVLLQHKIDSLEGGQVVNLTVQEK-TKETVLHYVLSKPSRGPRIQGGDYP-------------GCLNLLLECKDRSVVDELNRIMNWSDRQGNTALHYATQLWTQETVRKLLERGANIGVKNRWSETPISKILPETMESFLDEFCLT---STNDVQHEDFELKFRYSFLAPPVDSPNFDEHDPEGREMVENRALPETESLWYMADSQYHRHLLKHPVITSFLWMKWQRIRKYFNRGLRVYLLFVTCLSWFIFEAYGGVSYRVNSKTDTNSSSPESTESTVEEKEQRSLCS-VNLEPTESRGFWYVVFILQSIIQIFMILRDWYRDLRQSDLKTAFQDVLHPLIVKVLFTSWIEYFILSSIVVLIILSTQALVYILTILILMVALREVLQMMVSLKRYIFDIENLFELVMLALVCICLYVPEHYLYN----GCLLKRHLAGFALVIAWATLITLVGRHPKLSRYNIYVTMFYKVLKSFILFLTWYSFFIIAFGLGFYIMLHRDVP-----GYTPKEGEDEYKFFEDPWLSLVKTGTMFIGEIEFSNIPIDLTTKVGILGYIFLICFIFLIVVVLMNLLNGLAVSDTGIIREEAEIVTYISRVETISCTEAVLLGDPFNFLANWPAIAFLRDVPSMAFCRSLYRNS-LVRNISNKITGATGILLFYTWLPDKELVLQPNRRTNRF------------------VCMPTDCMGAEIIQAAKDIVIKKTRLGLEGPGEISD--LLLNLQKNQENL 1065          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325318857|ref|XP_023341191.1| (transient receptor potential cation channel subfamily A member 1-like [Eurytemora affinis])

HSP 1 Score: 722.62 bits (1864), Expect = 0.000e+0
Identity = 416/982 (42.36%), Postives = 571/982 (58.15%), Query Frame = 0

Query:   20 SC-QDAQSGVLDALRRDDPTTFSSILAEECHLDDTIFLRGSLSDDEKKKLLLPLDHWINQPILEEGEKTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLEN--PFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQVVVKEKPRE-----NTLDYLMEMLERR---------DLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPPI---PEEQLEEW-DDEQQSRL------EKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMR-------------CSSCSKCAWS-VLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPD-------------SALS---SDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQW 944
            SC  D  S  L +LR  D  TF+ IL  E              D   +   LP +HWIN PI EE  +TLL  A+ +    F + LLA GA ANLYN +L   PIH A + G  + +  LL +    N+ D N A+   G+TA+H+A +   +D+++ LLQ     VN KD KG QTPL+LA   ++ + V++LLE GA+L D+   K +++ I E +P  D+S+V +   P E     NT   L+ ++++          DL  FK++L   +   +S+    G +++QK C+ GLD++VE LL+ G++ NY   E+   PVLLA  RGH  VL+V  +YN          +  V  + T ETILH +L K             E     D +    L + V+DP    + Q++ +INKKD  GN+ +HYA Q W Q  VR L++ GANIG +N  G+IP+ KI PET+  FLNE C+   Q  +   HEDF + F Y+FLAPP+   P E L  + DDE+  ++      ++  LPET+++WY+ Q+K HRHL+KHPVITSFLW KW RIRR+FNRN+RFY  FV  LTWY+F  FGG SL+                             SE   +  WY +F++ S   ++   +DW  +++               S SK  WS +  +W E+  L+ + + LW+    L++VL + L  L LRE+FQ+A S++RY L LENWIE+ +I  + ++L  PD             SA+    +  E   D+KRH+ A++LL +W+ELIT + +HPRL  YN+YVTMFYKVL TFFFFLLWY FF++AF L FYIMLH+D  +              Y FF+SPWL+LVKTSTMF+GE+EFSDIP+NL   + P+ YLF L+FVFLIVVVLMNLLNGLAVSDTG IQE +EIVSY+SRV+TISYAES+LLGDPFDF S W
Sbjct:   22 SCVGDPSSTALQSLRSRDLRTFTDILNTE-------------EDVGSESSPLPEEHWINTPIQEENGETLLSSAISNHLHHFVQVLLAAGAQANLYNHQLANYPIHVAARVGERKCIELLLNSGCKTNRGDVN-AVNKVGRTALHVAVERNDVDLVDYLLQVPGVRVNPKDKKGGQTPLYLAVLNKSPQLVEMLLEQGANLQDICFGKCIQDLISEKMPDLDISRVKLNRAPLERENSSNTTQILLGLVDKAGAQSKPNQADLQTFKSLLLQFDMKTLSSIKTGGISLLQKCCVNGLDEFVEALLEEGLDPNYCPPESYSAPVLLAGGRGHLQVLQVLSKYN---------ADFSVLKKQTDETILHRVLIKD------------EHFENGDYQGCLDLLLSVNDPD--LKDQIDRIINKKDIFGNTALHYATQKWDQNIVRSLLERGANIGIKNRWGEIPIQKIDPETMEAFLNEYCL---QSTNDVNHEDFELVFKYNFLAPPLENLPTEVLGPYSDDEENHKISDRDIQKRYVLPETESLWYMGQTKEHRHLLKHPVITSFLWCKWNRIRRYFNRNLRFYLLFVTILTWYVFETFGGDSLR-----------------------------SEDGSIKAWYYIFSVFSAAMLIFIIKDWISDVKDLVYQESILEGSVRISGSKSYWSLIFSNWLEVVYLAGLAVILWLGPTCLYIVLLVLLCGLALREVFQVAVSIRRYILDLENWIEVGMIICISIVLLRPDVVQLDTGETNNGTSAVQYSENQFEQERDLKRHLAALSLLFSWSELITFIAKHPRLNIYNVYVTMFYKVLSTFFFFLLWYIFFILAFALSFYIMLHRDFETSP---------ESEYVFFDSPWLSLVKTSTMFIGELEFSDIPVNLDSKMVPLSYLFFLSFVFLIVVVLMNLLNGLAVSDTGAIQEHSEIVSYLSRVDTISYAESLLLGDPFDFFSSW 925          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325279050|ref|XP_023324174.1| (transient receptor potential cation channel protein painless-like [Eurytemora affinis])

HSP 1 Score: 572.392 bits (1474), Expect = 0.000e+0
Identity = 383/1018 (37.62%), Postives = 560/1018 (55.01%), Query Frame = 0

Query:   27 GVLDALRRDDPTTFSSILAEECHLDDTIFLRGSLSDDEKKKLLLPLDHWINQPILEEGEKTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNM--AMQPSGQTAVHIASDL---GKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVG--TKSVKEAIKENLPYFDVSQVVVKEKPRE---NTLDYLMEMLERRDLAYFKAVLRFLEPSEVSTS---GVRGF-TVMQKACIEGLDDYVEVLLDAGVNSN-YGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHE-DFSVTFHYDFLAP-----PIP-------EEQLEEWDDE----QQSRLEKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMR--CSSCSK---------------CAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGILLFFSYLPTKQET 995
            G L AL+ +D   F+ IL  +   D    +R  +S             WI  PI  E    L+++ ++ +N++F R L+  G  ++L N     AP+H AV  G  E V   ++N   + D N        G TA+H+ +D      L  L+ LL     +VN  D     +PL+LAAK++N  AV+ L+E+GA+    VG  +K+V++ IKENLP   +  + VK KPR+   N  + ++ ++             FLE   +S S     RGF  +++K C +GL D+ +VL +   + N +G+   S  P+L AA R  + + +V  R       G+  + V      T ET+LH +LK ++ ++++             E +++ LEIL +      RR ++LV+N+KD  G +P+HYA +  +Q  VR L++ GANIG +N  G+IP+ +I P  L +FL++DC+      D   H+ +FS+TF Y FLAP     P P       +E L+  + E      ++  K ALPET+++WY+ QSK HRHL+KHPVITSFLW KW+R+R +FNRN+R Y  +V  LTW+IF +FGG            V  +  I ++                   ++V++     +  +L  +DWTR+++   SS  K                   +  + P++  +S ++   ++    L + L + L +L LREL Q+  S+KRY   LENWIE+ +I LV  I       L ++ E    + RH+ A+AL+ +W ELITM+GRHP+L   NIYVTMFYKVL +F  FL+W+S F++AFGLGFYIMLH DT + ++ G E +D       FN  WL+LVKTS MFVGE+EFSD+PI+  G L  + Y + L+FVFLIVVVLMNLLNGLAVSDTG I+ +AEI SYISRVETISY ES LLGDPFDFLS      + +  PS +L+   Y +    K      TGA G LLF++ LP K+ T
Sbjct:    6 GALVALKGNDLQAFTDILNTK---DVEEGVRDQVS-------------WIYSPIKSEKSLNLVELCVEEDNLEFIRTLVRLGVHSHLLNTITGYAPLHRAVLLGKHEIVKLFIDNSRTEFDVNSRSGRLKRGYTALHLVADNPTDNHLQCLDILLGCEGIDVNCLDCSSTVSPLYLAAKKKNESAVRKLIENGAAFDIKVGRTSKTVEDFIKENLPQLQLRTIRVK-KPRQREDNVKERMITIVSNVSPDSLNLHQLFLEFRHLSLSVHDASRGFEDLVEKCCRKGLHDFAQVLFNKNADPNRFGSGSLSC-PILDAAERSDYRLAQVLVR------AGAEMSQV---KERTEETVLHCLLK-NYNKDIE-------------EDFSRFLEILFNKDDIKIRRSIDLVLNRKDLTGVTPLHYATEKCSQNVVRALLRRGANIGIKNMWGEIPISRICPRLLEEFLDQDCII----GDGEVHDKEFSLTFKYSFLAPDPQSLPEPYRPPLELDEALQPLNQEVNKINDNKERKTALPETESLWYMGQSKQHRHLLKHPVITSFLWFKWERMRPYFNRNLRLYLLYVFILTWFIFEQFGG------------VGQKPAIRDA-------------------FFVLYIALCILTTLLIIKDWTRDLKEIISSPQKEDPDYGVRSSRRGQGLVSQMTSNIPDILQISGMVCIFFIQPSHLNIPLIVLLCILVLRELLQLLVSMKRYLCSLENWIEVAMIVLVVYI-------LCNNTEDDFAVNRHLAAIALVFSWTELITMIGRHPKLHHLNIYVTMFYKVLNSFLLFLMWFSMFIVAFGLGFYIMLHTDTVT-DISGLEDEDKN---PLFNKTWLSLVKTSAMFVGELEFSDLPIDSSGYLGMLAYCYFLSFVFLIVVVLMNLLNGLAVSDTGDIKAKAEIYSYISRVETISYMESTLLGDPFDFLSNVP--EFLKSIPSISLIRQCYRTGFCRKVFK---TGAKGFLLFYTVLPDKEFT 931          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1325314367|ref|XP_023338859.1| (transient receptor potential cation channel protein painless-like [Eurytemora affinis])

HSP 1 Score: 400.208 bits (1027), Expect = 5.840e-119
Identity = 338/1080 (31.30%), Postives = 507/1080 (46.94%), Query Frame = 0

Query:    1 MERVPLMTMNTSNKVCAQLSC-QDAQSGVLDALRRDDPTTFSSILAEECHLDDTIFLRGSLSDDEKKKLLLPLDHWINQPILEEGEKTLLQVALQHENVDFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIAS------DLGKLD----ILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVS--QVVVKEKPRENTLDYLMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDD-----PSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHEDFSVTFHYDFLAPPIPEEQL-----EEWDDEQQSRLEKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMRCSSCSKCAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGILLFFSYLPTKQETF-----------PIRRRSHKHNRGRYDDDEEVEEEDCCPCCHQDDGNYEDVLKSARAILLQR 1046
            +E  PL+ M   N +    SC  D Q   L A    D  TF   ++EE               D  K+    +   ++  IL+E E   +          F  ALL  G   +L N  L+  P H A++  +     ALL+               G TA+H+A+      D  +LD    I+  L+Q    ++N +D KG  TPL+ AA   + +AV LLL +GA+       + ++E  KENLP F++S  +     +P +N L  L+E+ +  +L  F A   +        S    FT++Q A  +G       LL+ G + N  T  N   P +LAA  G++ VL+VF  + +   +      +       + TILH ++++   +        P   +  D  Y + L++L +D       + + +  E +IN +D  G + +HYA Q   Q  ++ L++ GAN+G +N     P++KI P TL +FL+E    C       T +D  +TF Y+FLAPP+ ++++      + D E  +  +    PET+ +WYLS+SK HR L+KHPVI+SFL +KWQRIR F+  ++  Y FFV+ LT  I   +GG SL                                     F  V   + S V+      D   E          W V+           I LT    I +L + +S+                 KRYF    N+I++ ++    +++F        D  MS D +RH+ A+ ++ +W+E I +VG+HP L+     VTM + V+ TFF FLLWY+ F++AF   FYIM H D    E+           Y+FF+     +VKTS MFVGE+EFSDIP +      PI Y+  +AF+FLIVVVLMNLLNGLAVSDTGLI+EEAE++S  S+V+ ISY ESILL DP++FL+ W    +    PS +  + +    P L+    KITG   ILLF+  LP K  TF           P R+R      G+      ++               +DVL +A+AI+L+R
Sbjct:    4 IELEPLLKMKPKNPMQRVQSCVGDPQEEALSAYESKDYKTFVDCVSEEGF-------------DPNKEYGEDIPKTLSHLILDEAEGRDI----------FLLALLKNGGRTDLRNKTLETIPFHEAIRKKD----PALLKVSLLGVQNVNIQDGEGSTALHVATEELQNADESELDNLLEIISILIQVPGIDLNHEDLKGKCTPLYYAAAAASEKAVDLLLCYGAN----SEIEMIQEVFKENLPDFNISNYKTARTGRPLKNVLFNLIELAD--NLEGF-ANFTYNRDGIDWNSDNGQFTLLQYAAEQGRAKICGYLLENGADPNL-TGSNDTPPWILAAHSGYYKVLQVF--FEKLTDREIRMAMITTDQHHGQRTILHEVVRQHSPK-----LDNPYRDTNID--YEECLKVLFEDRKNMRGKAKYSKYTERIINFRDLHGETALHYATQQPNQAIIKLLLKHGANMGVKNKEEKPPVNKILPSTLQEFLDE----CVASQGIITDDDLRLTFDYNFLAPPLLDKEVLLNFETKRDVEGSAVGDTHPRPETEALWYLSESKDHRGLLKHPVISSFLLMKWQRIRTFYIISLLIYFFFVLFLTLLILLEYGGCSL-------------------------------------FSSVSTNIESEVKEEQEGLDKADESSSCRGDTTVWKVIVG---------IFLTFLAIIELLQIAVSV-----------------KRYFASSTNYIQVAILVGGAILIF--------DRSMSWDERRHLAALLIIFSWSEFIILVGQHPSLS---TNVTMLFTVVSTFFKFLLWYTAFIVAFAFSFYIMFHTDHKDGEV--------NPEYKFFDEIGTCIVKTSAMFVGELEFSDIPFSAN----PISYIIFVAFIFLIVVVLMNLLNGLAVSDTGLIREEAEVLSLKSQVDIISYWESILLNDPYNFLTSWP--KFLSALPSCSCCFWV-KRIPLLETFLSKITGGARILLFYECLPDKSATFYPNSGKTNSCIPYRKRIKVVPTGKNPASTPLQVR-------------KDVLDAAKAIILER 933          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1233190500|ref|XP_022196180.1| (transient receptor potential cation channel protein painless [Nilaparvata lugens] >gi|1233190503|ref|XP_022196181.1| transient receptor potential cation channel protein painless [Nilaparvata lugens])

HSP 1 Score: 337.806 bits (865), Expect = 8.948e-96
Identity = 281/955 (29.42%), Postives = 442/955 (46.28%), Query Frame = 0

Query:   87 TLLQVALQHENVD-FSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGTKSVKEAIKENLPYFDVSQVVVKEKPRENTLDYLMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGF-TVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHED-FSVTFHYDFLAPPIPEEQLEEWDDEQQSRL--------------EKEALPETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMRCSSCSKCAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRH-MGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGIL--LFFSYLPTKQETFPIRRRSHKHNRGRYDDDEEVEEEDC 1021
            TLL +A +  +   + R LL+CGA  N  N    ++PIH AV+ G++  V  LL +  N+ D N  +  +G TA+H A+    +D++  LL   K N N  + KG  TP+ +A  +   EAV  LL+H  ++     T  + E+    +P    +   V+   + N L     +L + D   F   +R ++ +  S +   GF T +Q AC  G+   V  LL  G + N     N   P++LA+ +G+++++ +  +    C + +P            ET+LH ++K S   ++          S+ D  + K +E+L+           +L IN  D  GN+P+HYAA+   +E +  L+  GA +GQRN  G+  L  ++P+    +L +DC+T    +D    ED + + F Y+FLAPP    ++++ +D   SR                 + + ET+ + Y+S+S   R L+KHPV+TSFL LKW RIR FF  N+ FY FF + LT YI   + G  ++  G+ N K+                                               + + +   S  +     +G   + F+L L++              ++SLV+L LRELFQ+  S  RYF   ENW+E+ LI +   ILF            SC   RH + AVA+LL+WAEL+ ++GRHP L+     + M   V   F  FL WYS  +IAF L F+ +             EGD+      FF  P  ++ KT  M  GE +   IP  +  P     ++  + FVFLI +VL NLLNGLAVSDT  I+ +AE+V Y+SRV+ ++Y ES+++G P  +      +     C        +   C  L+  SH+I     +        LP +     +  R HK  R   +++ E    DC
Sbjct:   66 TLLDLACRKSSSSQYVRCLLSCGADPNKINSVRKKSPIHFAVELGDISIVNLLLHD--NRTDVN-KLDGTGSTALHAAARSSNVDVMIALLGHPKINPNIANRKG-LTPIHVAVTKGTEEAVLCLLQH-QNIDPRSATDLILESFPNLVPKLPTTGNTVETLGKSNNL---FNLLHKHDEETF---VRAIQINTDSINENDGFHTFLQYACDFGMFYTVGHLLRLGADPNGTHTANRTTPIMLASYKGYYNIVRLLVQ--NDCTRYNPING---------ETVLHCVIKGSTDLHVS------LGASKEDRDHYKCMEVLLRGVPGH-----KLNINCGDVKGNTPLHYAAKLNNKEFILLLLSSGAYVGQRNTLGEPALADVSPKIFEKYL-DDCLTT---NDKLPREDNYEIIFRYNFLAPP----KVQKHEDLTSSRAMLQIESNMKGVDEPTDQVISETEPLLYMSRSSDLRPLLKHPVLTSFLHLKWHRIRCFFYCNLTFYIFFWMFLTAYILTAYAG--MRPEGEGNDKINTNT-----------------------------------------TSFNKTLMKRSTQETIPETIGDEFKFFVLWLLV--------------ALSLVILILRELFQLTVSPLRYFANPENWLEVCLIGVTLSILFC----------RSCVNSRHQISAVAILLSWAELVLLIGRHPALS---TNIEMLKTVSWNFLKFLAWYSILIIAFALSFFTLFSDSET-------EGDEN-----FFVDPANSVFKTVVMLTGEFDAGSIPF-VTHP--GTSHVLFVLFVFLIAIVLFNLLNGLAVSDTQSIRADAELVGYVSRVKLVTYVESLVMGTPTPYRGFINKIQKLFCCLPRLDCCMVNYGC--LRIFSHRINLFPDLYPDCEVRILPNQASRIEMFSRGHKRKRQEIEEESESLFYDC 892          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1101344104|ref|XP_018897752.1| (PREDICTED: transient receptor potential cation channel protein painless isoform X3 [Bemisia tabaci])

HSP 1 Score: 332.028 bits (850), Expect = 4.441e-94
Identity = 287/977 (29.38%), Postives = 448/977 (45.85%), Query Frame = 0

Query:   87 TLLQVALQHENV-DFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGT---KSVKEAIKENLPYFDVSQVVVKEKPRENTLDY---LMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHED-FSVTFHYDFLAPP-IP---EEQLEEWDDEQQSRLEKEALP--ETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMRCSSCSKCAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGIL------LFFSYLPTKQETFPIRRRSHKHNRGRYDDDEEVEEEDCCPCCHQDDGNYEDVLKSARAIL 1043
            TLL +A Q  N  DF R L+  GA  N  N    +AP+H AV+  +++ +  LL++P    D N+ +  +G TA+H A  L KLD ++ LL   K  VNA  NK +QT + LA  + N   V  LL+H     D + T   +S++E I + LP    S    KE   ++  D    L   L + DL  F  +++  E  ++  +     T +Q AC  GL D+V +L+  G + N     NS   ++LA  +G++ ++++        +  +P            E+IL  ++K S   N   +  T E        Y K L++++ +  +      +L IN+ D  GN+ +HYAA+   Q+ +  L+  GA IG RN  G+  L  + P+ L  ++++    C   +D    ED F + F Y+FL PP +P   +      +D + +  +K  LP  ETD + Y+S+S   R L+ HPV+TSF+ LKW  IRRFF  N+ FYA F + LT YI   +   S    G+ +  V ++ G  +S PP          +S L   +                             CA ++L                   +GIL L           RELFQ+  S  RY + +ENW+E++LI +  MILF   +  S         +    A+ +LL+W EL+ ++GRHP L+     + MF  V   F  FL WYS  ++AF L FY +               D G E   F + P +++ KT  M  GE +   IP    G      ++  + FVFLI +VL NLLNGLAVSDT  I+ +AE+V ++SRV+ ISY E++ LG+P       A +     C   +       +C + + L+   +  T +            LP ++    + +  H+  R     D +  E  C   C Q+      ++K+A+ IL
Sbjct:   62 TLLDIACQALNYSDFVRILVQNGANVNQVNKTRKKAPLHFAVELSDIKTLQYLLDSP--NIDVNI-LDNTGGTALHSACRLEKLDQVKTLLNHRKIRVNAL-NKKNQTAIQLAILKENKTIVSELLKHPDIDIDSIKTLSGESIRELITDRLPDLVSSLPSPKEHSADDKSDKVSKLFSYLRQHDLDSFAKLIK--EDPKLVNADDGDHTFLQYACEFGLYDFVALLVRCGADVNATCATNSKTGLVLACKQGYYKIVQLL--IGNDAVSLAPVN---------EESILQTVIKNSTSENASFLGATNE-----GRDYFKCLKVILRNAPAN-----QLNINQGDLKGNTVLHYAAKTGNQDLIFLLLDHGAYIGTRNVFGEPALADLPPKILEAYMDQ----CVGTNDKLPREDNFEIIFKYNFLVPPKVPIHGKSNSNAIEDVESN--DKAVLPTSETDPLLYMSRSPELRPLLIHPVLTSFVNLKWYSIRRFFYYNLAFYASFWLLLTVYILGFYDSGS----GNLDLNVENKTGSWDSFPP-------LKTKSDLSLLW-----------------------------CALAIL-------------------LGILVL-----------RELFQLFVSPVRYIITIENWLEIFLIIVTAMILFGNPTRNS---------RNQTSAIGILLSWGELVLLIGRHPSLS---TNIEMFKTVSNNFLKFLAWYSILILAFALSFYSLFK-------------DAGTEDSNFLD-PGMSIFKTVVMLTGEFDSGSIPF---GSNPGTSHILFVLFVFLIAIVLFNLLNGLAVSDTQEIRADAELVGHVSRVKLISYIETMALGNPVPIRGFAAQLKSMCCCVPDSFF-----ACNSNRHLARIFSRRTNLFPDSIPDYEIHVLPNQENKVDLAQTGHRRKR----QDLDSYESSCFATC-QNFMMEGSIVKAAKEIL 896          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1101344102|ref|XP_018897751.1| (PREDICTED: transient receptor potential cation channel protein painless isoform X2 [Bemisia tabaci])

HSP 1 Score: 331.257 bits (848), Expect = 7.666e-94
Identity = 287/977 (29.38%), Postives = 448/977 (45.85%), Query Frame = 0

Query:   87 TLLQVALQHENV-DFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGT---KSVKEAIKENLPYFDVSQVVVKEKPRENTLDY---LMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHED-FSVTFHYDFLAPP-IP---EEQLEEWDDEQQSRLEKEALP--ETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMRCSSCSKCAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGIL------LFFSYLPTKQETFPIRRRSHKHNRGRYDDDEEVEEEDCCPCCHQDDGNYEDVLKSARAIL 1043
            TLL +A Q  N  DF R L+  GA  N  N    +AP+H AV+  +++ +  LL++P    D N+ +  +G TA+H A  L KLD ++ LL   K  VNA  NK +QT + LA  + N   V  LL+H     D + T   +S++E I + LP    S    KE   ++  D    L   L + DL  F  +++  E  ++  +     T +Q AC  GL D+V +L+  G + N     NS   ++LA  +G++ ++++        +  +P            E+IL  ++K S   N   +  T E        Y K L++++ +  +      +L IN+ D  GN+ +HYAA+   Q+ +  L+  GA IG RN  G+  L  + P+ L  ++++    C   +D    ED F + F Y+FL PP +P   +      +D + +  +K  LP  ETD + Y+S+S   R L+ HPV+TSF+ LKW  IRRFF  N+ FYA F + LT YI   +   S    G+ +  V ++ G  +S PP          +S L   +                             CA ++L                   +GIL L           RELFQ+  S  RY + +ENW+E++LI +  MILF   +  S         +    A+ +LL+W EL+ ++GRHP L+     + MF  V   F  FL WYS  ++AF L FY +               D G E   F + P +++ KT  M  GE +   IP    G      ++  + FVFLI +VL NLLNGLAVSDT  I+ +AE+V ++SRV+ ISY E++ LG+P       A +     C   +       +C + + L+   +  T +            LP ++    + +  H+  R     D +  E  C   C Q+      ++K+A+ IL
Sbjct:   70 TLLDIACQALNYSDFVRILVQNGANVNQVNKTRKKAPLHFAVELSDIKTLQYLLDSP--NIDVNI-LDNTGGTALHSACRLEKLDQVKTLLNHRKIRVNAL-NKKNQTAIQLAILKENKTIVSELLKHPDIDIDSIKTLSGESIRELITDRLPDLVSSLPSPKEHSADDKSDKVSKLFSYLRQHDLDSFAKLIK--EDPKLVNADDGDHTFLQYACEFGLYDFVALLVRCGADVNATCATNSKTGLVLACKQGYYKIVQLL--IGNDAVSLAPVN---------EESILQTVIKNSTSENASFLGATNE-----GRDYFKCLKVILRNAPAN-----QLNINQGDLKGNTVLHYAAKTGNQDLIFLLLDHGAYIGTRNVFGEPALADLPPKILEAYMDQ----CVGTNDKLPREDNFEIIFKYNFLVPPKVPIHGKSNSNAIEDVESN--DKAVLPTSETDPLLYMSRSPELRPLLIHPVLTSFVNLKWYSIRRFFYYNLAFYASFWLLLTVYILGFYDSGS----GNLDLNVENKTGSWDSFPP-------LKTKSDLSLLW-----------------------------CALAIL-------------------LGILVL-----------RELFQLFVSPVRYIITIENWLEIFLIIVTAMILFGNPTRNS---------RNQTSAIGILLSWGELVLLIGRHPSLS---TNIEMFKTVSNNFLKFLAWYSILILAFALSFYSLFK-------------DAGTEDSNFLD-PGMSIFKTVVMLTGEFDSGSIPF---GSNPGTSHILFVLFVFLIAIVLFNLLNGLAVSDTQEIRADAELVGHVSRVKLISYIETMALGNPVPIRGFAAQLKSMCCCVPDSFF-----ACNSNRHLARIFSRRTNLFPDSIPDYEIHVLPNQENKVDLAQTGHRRKR----QDLDSYESSCFATC-QNFMMEGSIVKAAKEIL 904          

BLAST of transient receptor potential cation channel protein painless vs. nr
Match: gi|1101344100|ref|XP_018897750.1| (PREDICTED: transient receptor potential cation channel protein painless isoform X1 [Bemisia tabaci])

HSP 1 Score: 331.643 bits (849), Expect = 7.899e-94
Identity = 287/977 (29.38%), Postives = 448/977 (45.85%), Query Frame = 0

Query:   87 TLLQVALQHENV-DFSRALLACGAIANLYNDELDQAPIHNAVQSGNLEHVVALLENPFNKADPNMAMQPSGQTAVHIASDLGKLDILERLLQESKANVNAKDNKGHQTPLFLAAKRRNAEAVKLLLEHGASLTDLVGT---KSVKEAIKENLPYFDVSQVVVKEKPRENTLDY---LMEMLERRDLAYFKAVLRFLEPSEVSTSGVRGFTVMQKACIEGLDDYVEVLLDAGVNSNYGTKENSWRPVLLAAARGHFDVLEVFKRYNQKCMKGSPQTNVGVWTRDTRETILHLILKKSFRRNLQGIYPTPEEISRWDEKYTKSLEILVDDPSSAFRRQLELVINKKDNMGNSPIHYAAQYWTQETVRQLMQLGANIGQRNWRGDIPLHKIAPETLADFLNEDCVTCPQGHDPNTHED-FSVTFHYDFLAPP-IP---EEQLEEWDDEQQSRLEKEALP--ETDTIWYLSQSKAHRHLIKHPVITSFLWLKWQRIRRFFNRNMRFYAFFVVALTWYIFARFGGHSLQLRGDQNFKVAHEVGIEESRPPGFCSPIQWSERSYLGFWYVVFALHSCVQVVLAYRDWTREMRCSSCSKCAWSVLGSWPELFILSLIILTLWMTIGILWLVLSISLVLLGLRELFQMASSLKRYFLCLENWIELWLIALVGMILFIPDSALSSDLEMSCDIKRHMGAVALLLAWAELITMVGRHPRLARYNIYVTMFYKVLETFFFFLLWYSFFLIAFGLGFYIMLHQDTASKELGGGEGDDGGESYEFFNSPWLALVKTSTMFVGEIEFSDIPINLKGPLAPIGYLFLLAFVFLIVVVLMNLLNGLAVSDTGLIQEEAEIVSYISRVETISYAESILLGDPFDFLSQWAPVSWARMCPSGTLLYHLYNSCPALKRLSHKITGATGIL------LFFSYLPTKQETFPIRRRSHKHNRGRYDDDEEVEEEDCCPCCHQDDGNYEDVLKSARAIL 1043
            TLL +A Q  N  DF R L+  GA  N  N    +AP+H AV+  +++ +  LL++P    D N+ +  +G TA+H A  L KLD ++ LL   K  VNA  NK +QT + LA  + N   V  LL+H     D + T   +S++E I + LP    S    KE   ++  D    L   L + DL  F  +++  E  ++  +     T +Q AC  GL D+V +L+  G + N     NS   ++LA  +G++ ++++        +  +P            E+IL  ++K S   N   +  T E        Y K L++++ +  +      +L IN+ D  GN+ +HYAA+   Q+ +  L+  GA IG RN  G+  L  + P+ L  ++++    C   +D    ED F + F Y+FL PP +P   +      +D + +  +K  LP  ETD + Y+S+S   R L+ HPV+TSF+ LKW  IRRFF  N+ FYA F + LT YI   +   S    G+ +  V ++ G  +S PP          +S L   +                             CA ++L                   +GIL L           RELFQ+  S  RY + +ENW+E++LI +  MILF   +  S         +    A+ +LL+W EL+ ++GRHP L+     + MF  V   F  FL WYS  ++AF L FY +               D G E   F + P +++ KT  M  GE +   IP    G      ++  + FVFLI +VL NLLNGLAVSDT  I+ +AE+V ++SRV+ ISY E++ LG+P       A +     C   +       +C + + L+   +  T +            LP ++    + +  H+  R     D +  E  C   C Q+      ++K+A+ IL
Sbjct:   75 TLLDIACQALNYSDFVRILVQNGANVNQVNKTRKKAPLHFAVELSDIKTLQYLLDSP--NIDVNI-LDNTGGTALHSACRLEKLDQVKTLLNHRKIRVNAL-NKKNQTAIQLAILKENKTIVSELLKHPDIDIDSIKTLSGESIRELITDRLPDLVSSLPSPKEHSADDKSDKVSKLFSYLRQHDLDSFAKLIK--EDPKLVNADDGDHTFLQYACEFGLYDFVALLVRCGADVNATCATNSKTGLVLACKQGYYKIVQLL--IGNDAVSLAPVN---------EESILQTVIKNSTSENASFLGATNE-----GRDYFKCLKVILRNAPAN-----QLNINQGDLKGNTVLHYAAKTGNQDLIFLLLDHGAYIGTRNVFGEPALADLPPKILEAYMDQ----CVGTNDKLPREDNFEIIFKYNFLVPPKVPIHGKSNSNAIEDVESN--DKAVLPTSETDPLLYMSRSPELRPLLIHPVLTSFVNLKWYSIRRFFYYNLAFYASFWLLLTVYILGFYDSGS----GNLDLNVENKTGSWDSFPP-------LKTKSDLSLLW-----------------------------CALAIL-------------------LGILVL-----------RELFQLFVSPVRYIITIENWLEIFLIIVTAMILFGNPTRNS---------RNQTSAIGILLSWGELVLLIGRHPSLS---TNIEMFKTVSNNFLKFLAWYSILILAFALSFYSLFK-------------DAGTEDSNFLD-PGMSIFKTVVMLTGEFDSGSIPF---GSNPGTSHILFVLFVFLIAIVLFNLLNGLAVSDTQEIRADAELVGHVSRVKLISYIETMALGNPVPIRGFAAQLKSMCCCVPDSFF-----ACNSNRHLARIFSRRTNLFPDSIPDYEIHVLPNQENKVDLAQTGHRRKR----QDLDSYESSCFATC-QNFMMEGSIVKAAKEIL 909