hypothetical protein DAPPUDRAFT_329527, maker-scaffold1323_size47899-snap-gene-0.11 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. L. salmonis genes
Match: EMLSAG00000009984 (supercontig:LSalAtl2s:LSalAtl2s653:17916:32674:1 gene:EMLSAG00000009984 transcript:EMLSAT00000009984 description:"maker-LSalAtl2s653-augustus-gene-0.25") HSP 1 Score: 1846.63 bits (4782), Expect = 0.000e+0 Identity = 919/1313 (69.99%), Postives = 1085/1313 (82.64%), Query Frame = 0
Query: 44 AGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDET--GSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIP-AIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGG-----LEKD----LDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGA-KGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKK---PWE-DSDATSESD 1339
A GG KRLSVERIYQKK+QLEHILLRPD+YIGSV P+TE M++++E ++VNK+IT+VPGLYKIFDEILVNAADNKQRD MDTIK+DI+ EKNEI+VFNNGRGIPV HKDEKMFVPT+IFGHLLTSSNFNDEEEKVTGGRNG+GAKLCNVFS+KFTVETAS+E +K FKQVW NMSKA E K+ +S G DYTR+TF+PDL KF+M SLD +TVALLSRRAYD+AASSKGVKVFLNGKRI +K FKDYVDFF+K +E+E S +K ++EA G RWEVA AV +NGF+Q SFVNSIATTKGGRHVD+V+ +I + E +KKKNKGG++IKP QI+NHLW+F+N LIVNPTFDSQTKENMTL + FGSKC + KF + KSG+VE+VLAWSKFK DQ +K S KKT+KLKGI KLEDANDAGT+NSIDCTLILTEGDSAKTLA AGL VVGRDKFGVYPLRGKLLNVREATHKQ++ENKEINE+VKI+GLTYKKKY T DD+KSLRYGK+MIMTDQDQDGSHIKGLLINFIHHNWP+LL+ PFLEEFITPIVKA+KG S F+SLPE EWK+ T NW ++KIKYYKGLGTSTSKEAKEYFSDM+RH+ILF ++ QDDH+I MAFSKKA+++RKEWLT WME+CKRRKELG++E+YLYEK+T++V+YS+F+NKELVLFSNLDNERSIPS+VDG KPGQRKV+FTCLKRNDKRE+KVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNN+NLLLPIGQFGTRLAGGKDHAS RYIFTQ++P+TR +F+ +DD VL YL DDN ++EPE+Y+PI+PMVL+NGADGIGTGWMTK+PNYNP++I++N+ ++I+G E ++M PWFK FKG IE I Q+YVVNGE+A++S+ KIE++ELPV++WTQ+YKE VME MLNGSEK P +II DYKEYHTDVTVKFVV M++DKLR+AE KGLHQFFKLQTTISTSSMVLFDA GCL+KY N LEI+ EF+ +R+ FYEKRK YL GMLEA++LKL NQARFI KC+GSLVVENKKRK +I +L KGFDSDPVK WK Q+++ D++ +E G +E+ DYDYL+ MAMWSLT+ERKDELLR+ EK EL ++ T ++D+W+ DLKEF K DE+ KE ED + + E KG KG K KI SP GIR+VP I +EL+ K AKAAAAK RK +K +K + V DEFD M KD N L +K ++ KG K S S P+ KGKK PWE DS + E D
Sbjct: 1714 AKGGQKKRLSVERIYQKKTQLEHILLRPDSYIGSVQPLTESMYVIEE--GKVVNKDITFVPGLYKIFDEILVNAADNKQRDPKMDTIKVDIDAEKNEIKVFNNGRGIPVVQHKDEKMFVPTMIFGHLLTSSNFNDEEEKVTGGRNGFGAKLCNVFSSKFTVETASKEYKKSFKQVWGANMSKASEPKITPYS-GEDYTRVTFSPDLEKFHMSSLDPNTVALLSRRAYDVAASSKGVKVFLNGKRIPIKGFKDYVDFFIKCYEEENPGASEIKPVFEACGPRWEVAXAVXENGFKQXSFVNSIATTKGGRHVDHVTDLITKALGETIKKKNKGGISIKPFQIKNHLWIFVNALIVNPTFDSQTKENMTLQVKSFGSKCVLSDKFNNLINKSGIVEAVLAWSKFKQDQMLKKNTSGKKTSKLKGIPKLEDANDAGTRNSIDCTLILTEGDSAKTLAXAGLSVVGRDKFGVYPLRGKLLNVREATHKQLLENKEINELVKIMGLTYKKKYETDDDMKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPTLLKRPFLEEFITPIVKASKGQSSFPFYSLPEFEEWKNSTPNWPSFKIKYYKGLGTSTSKEAKEYFSDMRRHRILFSYDGQQDDHSIQMAFSKKAVEERKEWLTNWMEDCKRRKELGMAEVYLYEKNTRSVTYSEFVNKELVLFSNLDNERSIPSLVDGLKPGQRKVVFTCLKRNDKREIKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNVNLLLPIGQFGTRLAGGKDHASARYIFTQLNPMTRYLFHIHDDYVLKYLRDDNLRVEPEYYVPILPMVLINGADGIGTGWMTKVPNYNPKDIIKNIKSLINGGEMKEMVPWFKHFKGSIEYIGEQKYVVNGEVANLSDTKIEISELPVKTWTQTYKESVMEQMLNGSEKNPSSIISDYKEYHTDVTVKFVVTMASDKLREAE-RKGLHQFFKLQTTISTSSMVLFDAYGCLKKYNNPLEIISEFYDVRIRFYEKRKKYLVGMLEADNLKLKNQARFIKMKCEGSLVVENKKRKDIILDLTNKGFDSDPVKCWKKVQEIDCDTEEQTEEGDDTINVEQSDDEKKDYDYLMSMAMWSLTKERKDELLRKAQEKQDELDAVKATLEKDMWKKDLKEFENKYDELMKKEKEDLAKLYAVE--KGVRKGGAKSKISTQASPHGIRIVPRIDDELRTKVAKAAAAKDRKAKKDKNPVVK--DEVPDEFDDMVTGKDGNEKLVDK----ISKKGVVKSSSRKSDSLSSPK-----KGKKRENPWESDSGSGGEED 3009
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|6094494|sp|O16140.1|TOP2_BOMMO (RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase II; Short=TOPOII) HSP 1 Score: 1619.75 bits (4193), Expect = 0.000e+0 Identity = 794/1259 (63.07%), Postives = 971/1259 (77.12%), Query Frame = 0
Query: 34 KANGEAK-KPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVP-----GLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKW-KNAQKME-----EDSDSDSEG---------GLEKDLD-----------YDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLK--IEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRK 1258
K NG + P G GA +E+IYQKKSQLEHILLRPDTYIGSV TE MW+ D+ + ++ KE+T VP GLYKI+DEILVNAADNKQRD MD IKIDIN+E N I V+NNG GIPV MHKDEKM+VPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FSTKFTVETAS++ +K FKQ W +NM+KA E K+KD D+T+I F+PDL KF ME L+ D V L+SRRAYD+AASS+GVKV+LNG+R+ + FKDYVD ++KG +DE G P+K +YE +RWEVA+ +SD GFQQ+SFVNSIATTKGG+HVD V+ + N+ EVLKKKNKGGV IKP+Q++ H+WLF+NCLIVNPTFDSQTKENMTL FGSKC+ + KF + K G+VESVL W+KFKA Q+ K S KK +KLKGI KLEDANDAGTKN+ CTLILTEGDSAKTLAV+GL VVGRD +GV+PL GK LNVR+A+HKQ++EN EIN ++KI+GL YKKKY++VDDLK+LRYGK+MIM DQDQDGSHIKGL+INFIHHNWP LL+LPFLEEFITPIVKATK SF+SLPE EWK T+N HTY IKYYKGLGTSTSKEAKEYF +M RH+I FK+ DDH I +AFSKK DQRKEWLT M+E KRRKE+GLSE YLY K+TKTV+YSDF+N ELVLFSN DN RSIPS++DG KPGQRKV+FTC+KRNDKREVKVAQLAGSV E SAYHHGE SL TI+NLAQN+VGSNNINLL P GQFGTRL+GGKD ASPRYIFT MSPLTRLIF+ +DD +L + +DNQKIEP +Y+PIIPMVLVNG +GIGTGW TKIPNYNPR+I NL ++DG +P+ M PW+K+FKG +E +YV++GE A + +KIE+TELPV +WT +YKE V+EPML G++K+ +I +Y+EY+TD TV+FVV + KL + E + G+H+ FKLQTTIS + M FD CL KY+ V EI+REF+ LR+ +Y +RK+YLEG L+AE+ KLSNQARFIL+KCD LV+ENKKRKA++EEL ++G+ DP+ W K A K++ ED D+ G D D ++YLLGM+MW LT+E+KDELL+++D+K EL L+ + LWR DL F+ KLDEVE KE +E + ++K++ K GKK K + +PS G RV P I+++L + A AK+RK
Sbjct: 15 KQNGTGEPAPAKGQKGA----IEKIYQKKSQLEHILLRPDTYIGSVERATETMWVYDKVKECMMQKELTSVPDCSFPGLYKIYDEILVNAADNKQRDPKMDVIKIDINQETNTISVYNNGSGIPVVMHKDEKMYVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTKFTVETASKQYKKHFKQTWGSNMTKASEPKIKDSGKDDDFTKIVFSPDLAKFKMEKLEDDIVLLMSRRAYDVAASSQGVKVYLNGERLKINKFKDYVDLYIKGKDDENGQPLKVVYEKVSDRWEVALTISDMGFQQVSFVNSIATTKGGKHVDTVADSVVKNVLEVLKKKNKGGVNIKPYQVKTHMWLFVNCLIVNPTFDSQTKENMTLQAKSFGSKCNLSEKFITAVTKCGLVESVLTWAKFKA-QDQLVKASGKKQSKLKGIPKLEDANDAGTKNAHLCTLILTEGDSAKTLAVSGLSVVGRDHYGVFPLEGKPLNVRDASHKQVLENVEINNLIKILGLQYKKKYNSVDDLKTLRYGKVMIMADQDQDGSHIKGLIINFIHHNWPELLKLPFLEEFITPIVKATKKDKEISFYSLPEFEEWKKETENHHTYNIKYYKGLGTSTSKEAKEYFQNMDRHRIRFKYSGPTDDHHIELAFSKKGADQRKEWLTNHMDEVKRRKEIGLSERYLYTKETKTVTYSDFVNLELVLFSNGDNVRSIPSMIDGLKPGQRKVIFTCIKRNDKREVKVAQLAGSVAEHSAYHHGEQSLAMTIVNLAQNYVGSNNINLLEPRGQFGTRLSGGKDSASPRYIFTLMSPLTRLIFHPHDDPLLVHEFEDNQKIEPVYYLPIIPMVLVNGVEGIGTGWSTKIPNYNPRDIAENLRRLLDGEKPKVMHPWYKNFKGNVEGF-GDKYVISGEAAILPGDKIEITELPVGTWTHNYKENVLEPML-GTDKVKPLISEYREYNTDTTVRFVVSLLPGKLSEIEAE-GIHKVFKLQTTISMTCMNAFDYNCCLEKYDKVEEILREFYDLRVKYYVRRKDYLEGQLQAEADKLSNQARFILKKCDKGLVIENKKRKAMVEELIKRGYAPDPIADWKKRASKIQGLTALEDDDAQESEEEEPEPDPKGKPVDPDKAFQQLKEVKKFNYLLGMSMWMLTKEKKDELLKQRDQKLTELTTLKNKTAPMLWREDLDAFLIKLDEVEDKERREE-LNVNKKTSKAMAGKKNRKSMFDIIPSENGRRVEPKISDDLIKRIQAAEKAKTRK 1264
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|363548478|sp|Q01320.2|TOP2A_MOUSE (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme) HSP 1 Score: 1616.28 bits (4184), Expect = 0.000e+0 Identity = 792/1304 (60.74%), Postives = 991/1304 (76.00%), Query Frame = 0
Query: 48 GAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK---------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKL-GVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNS 1341
G KRLS+ERIYQKK+QLEHILLRPDTYIGSV VT++MW+ DE+ I +E+T+VPGLYKIFDEILVNAADNKQRD M I++ I+ E N I ++NNG+GIPV HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETASRE +K FKQ W +NM +A + +LK FS G DYT ITF PDL KF M+SLD D VAL+ RRAYDIA S+K VKVFLNG + VK F+ YVD +LK DETG+ +K I+E RWEV + +S+ GFQQ+SFVNSIAT+KGGRHVDYV+ I S + +V+KKKNKGGV +K HQ++NH+W+F+N LI NPTFDSQTKENMTL FGS C + KF G+VES+L W KFKA ++ K S+ K K+KGI KL+DANDAG++NS +CTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y D LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR FLEEFITPIVK +K +F+SLPE EWKS T N +K+KYYKGLGTSTSKEAKEYF+DMKRH+I FK+ +DD AI++AFSKK +D RKEWLT +ME+ ++RK LGL E YLY + T ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMS+YHHGE SLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPL RL+F DD L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW KIPN++ RE+V N+ ++DG EP M P +K+FKG IE + + +YV+NGE+A + IE++ELP+R+WTQ+YKEQV+EPMLNG+EK P++I DY+EYHTD TVKFV++M+ +KL +AE GLH+ FKLQ++++ +SMVLFD GCL+KY+ VL+I+R+FF LRL +Y RK +L GML AES KL+NQARFILEK DG +V+ENK +K LI+ L ++G+DSDPVK WK AQ+ + S S+ E ++YLL M +W LT+E+KDEL ++++EK QEL L+ S DLW+ DL F+E+L+ VEAKE +DE+V L ++ K K ++ + LPSP G RV+P + E+K +A K K RK K+ +N++ A +D SL +++ + GAKK+ + P K K + K PW DS++ S+ S
Sbjct: 23 GKKRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDED-VGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFS-GEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMSERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAE-RVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKAGKAKGKKAQMCADVLPSPRGKRVIPQVTVEMKAEAEK----KIRK--KIKSENVEGTPA---------EDGAEPGSLRQRIEKKQKKEPGAKKQTTLPFKPVK------KGRKKNPWSDSESDVSSNES 1302
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|6094498|sp|O46374.1|TOP2A_PIG (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme) HSP 1 Score: 1610.89 bits (4170), Expect = 0.000e+0 Identity = 847/1583 (53.51%), Postives = 1073/1583 (67.78%), Query Frame = 0
Query: 36 NGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK---------------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEEL--KNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSP-----KTKGKKPW---------------------EDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGSEVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKK---PKAKK--PKASPKVKKPPAAATKKSKKTSQGSDYDDSD-------------------EDDMFKDSDLSVLSVEKKVRGGVRRAAAQQKFTYSSDESDF 1551
N + K K KRLS+ERIYQKK+QLEHILLRPDTYIGSV VT++MW+ DE+ I +E+T+VPGLYKIFDEILVNAADNKQRD M I++ I+ E N I ++NNG+GIPV HK EKM+VP LIFG LLTSSN++DEE+KVTGGRNGYGAKLCN+FSTKFTVETASRE +K FKQ W +NM +A E +LK F+ G DYT ITF PDL KF M+SLD D VAL+ RRAYDIA S+K VKVFLNG ++ VK F+ YVD +LK DETG+P+K I+E RWEV + +S+ GFQQ+SFVNSIAT+KGGRHVDYV+ I + + +V+KKKNKGGV +K HQ++NH+W+F+N LI NPTFDSQTKENMTL + FGS C + KF G+VES+L W KFKA ++ K S+ K N++KGI KL+DANDAG +NS +CTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y D LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR FLEEFITPIVK +K +F+SLPE EWKS T N +K+KYYKGLGTSTSKEAKEYF+DMKRH+I FK+ +DD AI++AFSKK ID RKEWLT +ME+ ++RK LGL E YLY + T ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMS+YHHGE SLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPL RL+F DD L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW KIPN++ RE+V N+ ++DG EP M P +K+FKG IE + +YV++GE+A ++ IE++ELP+R+WTQ+YKEQV+EPMLNG+EK P +I DY+EYHTD TVKFVV+M+ +KL +AE GLH+ FKLQT+++ +SMVLFD GCL+KY+ VL+I+R+FF LRL +Y RK +L GML AES KL+NQARFILEK DG +++ENK +K LI+ L ++G+DSDPVK WK AQ+ + SDS ++ G ++YLL M +W LT+E+KDEL + ++EK QEL L+ S DLW+ DL F+E+L+ VEAKE +DE++ L + K K ++ E LPSP G RV+P + E+ + + KS E +++ EVE +K + KK+ + T K+ + P K K + PW E A +++ +V+ D +D ++K++ E S+ S + E + + PK +++T P+ FD DD+ ++ PS+P A P+ + + P+ KK T+ K K S KK PK K P V K P K ++ + S DDSD DD D DL+V S R R K+ SDE D
Sbjct: 12 NMQVNKTKKNEEAKKRLSIERIYQKKTQLEHILLRPDTYIGSVESVTQQMWVYDEDIG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFN-GEDYTCITFHPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDLYLKDKVDETGNPLKIIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVAKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQVKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRLLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAE-RVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKEADKSDSVADSGPT----FNYLLDMPLWYLTKEKKDELCKLRNEKEQELETLKRKSPSDLWKEDLAAFIEELEAVEAKEKQDEQIGLPGKGGKAKGKKTQMA-EVLPSPCGKRVIPRVTVEMKAEAEKKIKKKIKSENTEGSPQEDGMEVEGLKQRLE-------------------------KKQKREPGTKTKKQTTLPFKPIKKAKKRNPWSDSESDISSDESNFNVPPREKEPRRAAAKTKFTVDLDSDEDFSDADEKTRDEDFVPSDTSPQKAET------------SPKHTNKEPKPQKST-PSVSDFDADDAKDNVPP--SPSSPVADFPAVTE---------TIKPVSKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGAKKDPDLDSDVSKKPNPPKPKGRRKRKPSTSDDSDSNFEKMISKAVTSKKPKGESDDFHLDLDLAVAS-----RAKSGRTKKPIKYLEESDEDDL 1532
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|3122959|sp|O42131.1|TOP2B_CHICK (RecName: Full=DNA topoisomerase 2-beta; AltName: Full=DNA topoisomerase II, beta isozyme) HSP 1 Score: 1608.96 bits (4165), Expect = 0.000e+0 Identity = 788/1315 (59.92%), Postives = 991/1315 (75.36%), Query Frame = 0
Query: 49 AKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK--------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKI----EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMC--------DDKDMNRSLS----EKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESD 1339
+K++SVER+YQKK+QLEHILLRPDTYIGSV P+T+ MW+ DE+ + +E+T+VPGLYKIFDEILVNAADNKQRDKNM IKI I+ E N I ++NNG+GIPV HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETA +E + FKQ W NNM K E K+K F G DYT ITF PDL KF ME+LD D V+L++RRAYD+A S KGVKV LNGK++ V F+ YVD ++K DETG +K I+E ERW+V + +S+ GFQQ+SFVNSIATTKGGRHVDYV + + EV+KKKNK GV++KP Q++NH+W+F+NCLI NP+FDSQTKENMTL FGSKC + KFF G++ES+L W KFKA ++ K SS K +K+KGI KL+DANDAG K+S+DCTLILTEGDSAK+LAV+GLGV+GRD++GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y + LKSLRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLL+ FLEEFITPIVKA+K SF+S+PE EWK H +N +KIKYYKGLGTST+KEAKEYF+DM+RH+ILF++ +DD AI +AFSKK ID RKEWLT +ME+ ++R+ GL E +LY TK ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMSAYHHGE +LM TI+NLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPL RL+F S DD +L +L DDNQ++EPEWY+PIIPMVLVNGA+GIGTGW K+PNY+ REIV N+ M+DG +P M P +K+F+G I+ + +YVV+GEI + N +E+TELPVR+WTQ YKEQV+EPMLNG+EK PA+I DYKEYHTD TVKFVV+M+ +KL QAE GLH+ FKLQT+++ +SMVLFD GCL+KYE V +I++EFF LRL++Y RK +L GML AES KL+NQARFILEK G + +EN+ ++ LI+ L ++G++SDPVK WK AQ+ + D+ S G D++Y+L M++WSLT+E+ +EL++ +D K +EL L+ S DLW+ DL F+E+L++VEA+E ED + + KG GK K+K E +PSP G R+VP I +K A++ K KK + A+K EFD D +N + S KL + G + R + SST S+ K K + PW D ++ SESD
Sbjct: 51 SKKMSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDED-VGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFE-GDDYTCITFQPDLSKFKMENLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEA-AGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQITSAMKADASRKLLKK--------KKGDADSVAIKMEFDEEFGGVQAEGGGDDTVNTAASGTKTPKLKREKKEPGTRVRRAPSST----KSSAKKVKKRNPWSDDESKSESD 1350
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|20141946|sp|Q02880.3|TOP2B_HUMAN (RecName: Full=DNA topoisomerase 2-beta; AltName: Full=DNA topoisomerase II, beta isozyme) HSP 1 Score: 1608.58 bits (4164), Expect = 0.000e+0 Identity = 800/1339 (59.75%), Postives = 1001/1339 (74.76%), Query Frame = 0
Query: 22 NGEAKKANGEAKKANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK--MEEDS------DSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKES---QKGAKGKKKLKI-EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGK---------KPWEDSDATSESD 1339
N AKK E N +KK LSVER+YQKK+QLEHILLRPDTYIGSV P+T+ MW+ DE+ + +E+T+VPGLYKIFDEILVNAADNKQRDKNM IK+ I+ E N I ++NNG+GIPV HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETA +E + FKQ W NNM K EAK+K F G DYT ITF PDL KF ME LD D VAL++RRAYD+A S +GVKV NGK++ V F+ YVD ++K DETG +K I+E A ERW+V + +S+ GFQQ+SFVNSIATTKGGRHVDYV + + EV+KKKNK GV++KP Q++NH+W+FINCLI NPTFDSQTKENMTL FGSKC + KFF G+VES+L W KFKA ++ K SS K +K+KGI KL+DANDAG K+S++CTLILTEGDSAK+LAV+GLGV+GRD++GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y + LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLL+ FLEEFITPIVKA+K SF+S+PE EWK H +N +KIKYYKGLGTST+KEAKEYF+DM+RH+ILF++ +DD AI +AFSKK ID RKEWLT +ME+ ++R+ GL E +LY TK ++Y+DFINKEL+LFSN DNERSIPS+VDGFKPGQRKV+FTC KRNDKREVKVAQLAGSV EMSAYHHGE +LM TI+NLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT +S L RL+F + DD +L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW K+PNY+ REIV N+ M+DG +P M P +K+FKG I+ + +Y V+GEI + N +E+TELPVR+WTQ YKEQV+EPMLNG++K PA+I DYKEYHTD TVKFVV+M+ +KL QAE GLH+ FKLQTT++ +SMVLFD GCL+KYE V +I++EFF LRL++Y RK +L GML AES KL+NQARFILEK G + +EN+ +K LI+ L ++G++SDPVK WK AQ+ EED DS S+ G D++Y+L M++WSLT+E+ +EL++++D K +E+ L+ S DLW+ DL F+E+LD+VE++E ED +S ++ + G KKL++ E +PSP G R++P I A KA A S+K K K +L + AVK EFD + + E L SV K + P R + +P + GK PW D ++ SESD
Sbjct: 29 NNAAKKEESETANKNDSSKK----------LSVERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDED-VGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFD-GEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEA-AGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIIPEIT------AMKADA--SKKLLKKKKGDL-DTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINKGPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESD 1345
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|76363529|sp|Q64511.2|TOP2B_MOUSE (RecName: Full=DNA topoisomerase 2-beta; AltName: Full=DNA topoisomerase II, beta isozyme) HSP 1 Score: 1608.19 bits (4163), Expect = 0.000e+0 Identity = 796/1317 (60.44%), Postives = 996/1317 (75.63%), Query Frame = 0
Query: 49 AKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKW----------KNAQKMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKG------AKGKKKLKI-EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFD--FMCDDKDMN--RSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKK-----PWEDSDATSESD 1339
K+LSVER+YQKK+QLEHILLRPDTYIGSV P+T+ MW+ DE+ + +E+T+VPGLYKIFDEILVNAADNKQRDKNM IK+ I+ E N I ++NNG+GIPV HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETA +E + FKQ W NNM K EAK+K F G DYT ITF PDL KF ME LD D VAL++RRAYD+A S KGVKV NGK++ V F+ YVD ++K DETG +K I+E A ERW+V + +S+ GFQQ+SFVNSIATTKGGRHVDYV + S + EV+KKKNK GV++KP Q++NH+W+FINCLI NPTFDSQTKENMTL FGSKC + KFF G+VES+L W KFKA ++ K SS K +K+KGI KL+DANDAG K+S++CTLILTEGDSAK+LAV+GLGV+GRD++GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y + LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLL+ FLEEFITPIVKA+K SF+S+PE EWK H +N +KIKYYKGLGTST+KEAKEYF+DM+RH+ILF++ +DD AI +AFSKK ID RKEWLT +ME+ ++R+ GL E +LY TK ++Y+DFINKEL+LFSN DNERSIPS+VDGFKPGQRKV+FTC KRNDKREVKVAQLAGSV EMSAYHHGE +LM TI+NLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT +S L RL+F + DD +L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW K+PNY+ REIV N+ M++G +P M P +K+FKG I+ + +Y V+GEI + N +E+TELPVR+WTQ YKEQV+EPMLNG++K PA+I DYKEYHTD TVKFVV+M+ +KL QAE GLH+ FKLQTT++ +SMVLFD GCL+KYE V +I++EFF LRL++Y RK +L GML AES KL+NQARFILEK G + +EN+ +K LI+ L ++G++SDPVK W +++Q +DS SDS G D++Y+L M++WSLT+E+ +EL++++D K +E+ L+ S DLW+ DL F+E+LD+VEA+E ED IL+ S K KKL++ E +PSP G R+VP I A KA A SRK K KK + VK EFD F + +L+ V+ K +++ + +K P S+ KT KK PW D ++ SESD
Sbjct: 34 TKKLSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDED-VGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFD-GEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEA-AGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDS--GTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQERED---ILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEIT------AMKADA--SRKLLK-KKKGDPDTTVVKVEFDEEFSGTPAEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPTSTGKTNAKKVKKRNPWSDDESKSESD 1333
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|1174741|sp|P41515.1|TOP2A_CRIGR (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme) HSP 1 Score: 1607.81 bits (4162), Expect = 0.000e+0 Identity = 835/1479 (56.46%), Postives = 1053/1479 (71.20%), Query Frame = 0
Query: 50 KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK-------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKK--PWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGSEVSDYEPEAKIAKNMAQNDIFNSSD-----------DSDAPK-------VKRATAPAHKVFDLDDSDEDFDKV-LKPSAPAAK--------RPSSDQ----KSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKK 1488
KRLS+ERIYQKK+QLEHILLRPDTYIGSV VT++MW+ DE+ I +E+T+VPGLYKIFDEILVNAADNKQRD M I++ I+ E N I ++NNG+GIPV HK EKM+VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FST+FTVETAS+E +K FKQ W +NM +A + +LK F+ G DYT ITF PDL KF M+SLD D VAL+ RRAYDIA S+K VKVFLNG ++ VK F+ YVD +LK DETG+ +K ++E RWEV + +S+ GFQQ+SFVNSIAT+KGGRHVDYV+ I S + +V+KKKNKGGV +K HQ++NH+W+F+N LI NPTFDSQTKENMTL FGS C + KF G+VES+L W KFKA ++ K S+ K N++KGI KL+DANDAG++NS +CTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y D LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR FLEEFITPIVK +K +F+SLPE EWKS T N +K+KYYKGLGTSTSKEAKEYF+DMKRH+I FK+ +DD AI++AFSKK +D RKEWLT +ME+ ++RK LGL E YLY + T ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMS+YHHGE SLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPLTRL+F DD L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW KIPN++ RE+V N+ ++DG EP M P +K+FKG IE + + +YV+NGE+A ++ IE++ELP+R+WTQ+YKEQV+EPMLNG+EK P +I DY+EYHTD TVKFV++M+ +KL +AE GLH+ FKLQT+++ +SMVLFD GCL+KY+ VL+I+++FF LRL +Y RK +L GML AES KL+NQARFILEK DG +++ENK +K LI+ L ++G+DSDPVK WK AQ+ EE + +S+ E ++YLL M +W LT+E+KDEL ++++EK QEL L+ S DLW+ DL F+E+L+ VEAKE +DE+V L + K K ++ E LPSP G RV+P + E+K +A K K RK K+ +N VE E SL +++ +K KK + + P KGKK PW D SESD S ++S D + +P+ + +AK ++ ++ F+ SD D+ PK K+A P D ++ D V P PAA +PSS Q K S S S KKR + K K + P +KP K K S K K+
Sbjct: 25 KRLSIERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDED-VGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTRFTVETASKEYKKMFKQTWMDNMGRAGDMELKPFN-GEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNALKVVHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQELAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLTRLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDIREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMTEEKLAEAE-RVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILKDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNENSDSVAESGPTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKNKSPSDLWKEDLAVFIEELEVVEAKEKQDEQVGLPGKGGKAKGKKAQMS-EVLPSPHGKRVIPQVTMEMKAEAEK----KIRK--KIKSEN---VEGTPTENGLELG------SLKQRI-----EKKQKKEPGAMTKKQTTLAFKPIKKGKKRNPWSD----SESDMSS-----NESNVDVPPREKDPRRAAT------KAKFTMDLDSDEDFSGSDGKDEDEDFFPLDTTPPKTKIPQKNTKKALKPQKSAMSGDPESDEKDSVPASPGPPAADLPADTEQLKPSSKQTVAVKKTATKSQSSTSTAGTKKRAVPKGSKSDSALNAHGP-EKPVPAKAKNSRKRKQ 1463
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|3024753|sp|Q64399.1|TOP2B_CRILO (RecName: Full=DNA topoisomerase 2-beta; AltName: Full=DNA topoisomerase II, beta isozyme) HSP 1 Score: 1607.04 bits (4160), Expect = 0.000e+0 Identity = 802/1352 (59.32%), Postives = 998/1352 (73.82%), Query Frame = 0
Query: 14 ANGDTQKANGEAKKANGEAKKANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKW----------KNAQKMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKG------AKGKKKLKI-EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGK---------KPWEDSDATSESD 1339
A+G N AKK E N +KK LSVER+YQKK+QLEHILLRPDTYIGSV P+T+ MW+ DE+ + +E+T+VPGLYKIFDEILVNAADNKQRDKNM IK+ I+ E N I ++NNG+GIPV HK EK++VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETA +E + FKQ W NNM K EAK+K F G DYT ITF PDL KF ME LD D VAL++RRAYD+A S KGVKV NGK++ V F+ YVD ++K DETG +K I+E A ERW+V + +S+ GFQQ+SFVNSIATTKGGRHVDYV + + EV+KKKNK GV++KP Q++NH+W+FINCLI NPTFDSQTKENMTL FGSKC + KFF G+VES+L W KFKA ++ K SS K +K+KGI KL+DANDAG K+S++CTLILTEGDSAK+LAV+GLGV+GRD++GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y + LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLL+ FLEEFITPIVKA+K SF+S+PE EWK H +N +KIKYYKGLGTST+KEAKEYF+DM+RH+ILF++ +DD AI +AFSKK ID RKEWLT +ME+ ++R+ GL E +LY TK ++Y+DFINKEL+LFSN DNERSIPS+VDGFKPGQRKV+FTC KRNDKREVKVAQLAGSV EMSAYHHGE +LM TI+NLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT +S L RL+F + DD +L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW K+PNY+ REIV N+ M+DG +P M P +K+FKG I+ + +Y V+GEI + N +E+TELPVR+WTQ YKEQV+EPMLNG++K PA+I DYKEYHTD TVKFVV+M+ +KL QAE GLH+ FKLQTT++ +SMVLFD GCL+KYE V +I++EFF LRL++Y RK +L GML AES KL+NQARFILEK G + +EN+ +K LI+ L ++G++SDPV+ W ++ Q +DS SDS G D++Y+L M++WSLT+E+ +EL++++D K +E+ L+ S DLW+ DL F+E+LD+VEA+E ED IL+ S K KKL++ E +PSP G R+VP I A KA A SRK K KK + VK EFD + + E L S K + P R + +P + GK PW D ++ SESD
Sbjct: 9 ADGALTWVNNAAKKEELETSNKNDSSKK----------LSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDED-VGMNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFD-GEDYTCITFQPDLAKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLSEKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEA-AGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVRAWKEAQEKAAEEEDTQNQHDDSSSDS--GTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQERED---ILAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVPEIT------AMKADA--SRKLLK-KKKGDPDTPVVKVEFDEEFSGTPVEGTGEETLTPSAPVNKGPKPKREKKEPGTRVRKTPTSAGKPNAKKVKKRNPWSDDESKSESD 1333
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|13959709|sp|P11388.3|TOP2A_HUMAN (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme) HSP 1 Score: 1602.8 bits (4149), Expect = 0.000e+0 Identity = 853/1565 (54.50%), Postives = 1069/1565 (68.31%), Query Frame = 0
Query: 36 NGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK---------------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEEL--KNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGSEVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHK--------VFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKK---PKAKK--PKASPKVKKPPAAATKKSKKTSQGSDYDDSD-------------------EDDMFKDSDLSVLSVEKKVRGGVRRAAAQQKFTYSSDESDF 1551
N + K K KRLSVERIYQKK+QLEHILLRPDTYIGSV VT++MW+ DE+ I +E+T+VPGLYKIFDEILVNAADNKQRD M I++ I+ E N I ++NNG+GIPV HK EKM+VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETASRE +K FKQ W +NM +A E +LK F+ G DYT ITF PDL KF M+SLD D VAL+ RRAYDIA S+K VKVFLNG ++ VK F+ YVD +LK DETG+ +K I+E RWEV + +S+ GFQQ+SFVNSIAT+KGGRHVDYV+ I + + +V+KKKNKGGV +K HQ++NH+W+F+N LI NPTFDSQTKENMTL FGS C + KF G+VES+L W KFKA ++ K S+ K N++KGI KL+DANDAG +NS +CTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y D LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR FLEEFITPIVK +K +F+SLPE EWKS T N +K+KYYKGLGTSTSKEAKEYF+DMKRH+I FK+ +DD AI++AFSKK ID RKEWLT +ME+ ++RK LGL E YLY + T ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMS+YHHGE SLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +S L RL+F DD L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW KIPN++ REIV N+ ++DG EP M P +K+FKG IE + +YV++GE+A ++ IE++ELPVR+WTQ+YKEQV+EPMLNG+EK P +I DY+EYHTD TVKFVV+M+ +KL +AE GLH+ FKLQT+++ +SMVLFD GCL+KY+ VL+I+R+FF LRL +Y RK +L GML AES KL+NQARFILEK DG +++ENK +K LI+ L ++G+DSDPVK WK AQ+ E SDS ++ G ++YLL M +W LT+E+KDEL R ++EK QEL L+ S DLW+ DL F+E+L+ VEAKE +DE+V L + K K ++ E LPSP G RV+P I E+ + + K+ E +++ E+E +K +K R K KK+ + + P K K K + PW DS++ SD S +FD + + ++ K+ + E + +D D+ PK K + ++K V DL+ D L S PA P D + +P+ KK T+ K K S KK PK K P + V + P A K+++ + S DDSD DD D D +V K V RA K+ SDE D
Sbjct: 12 NMQVNKIKKNEDAKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDED-VGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFN-GEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAHQVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAE-RVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPT----FNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAVEAKEKQDEQVGLPGKGGKAKGKKTQMA-EVLPSPRGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRL-----EKKQKREPGTK---------TKKQTTLAFKPIK------KGKKRNPWSDSESDRSSDES----NFDVPPRETEPRRAATKTKFTMDLDSDEDFSDFDEKTDDEDFVPSDASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFP---------DETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSV-----RAKKPIKYLEESDEDDL 1530
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Match: gi|1174742|sp|P41516.1|TOP2A_RAT (RecName: Full=DNA topoisomerase 2-alpha; AltName: Full=DNA topoisomerase II, alpha isozyme) HSP 1 Score: 1599.72 bits (4141), Expect = 0.000e+0 Identity = 829/1487 (55.75%), Postives = 1051/1487 (70.68%), Query Frame = 0
Query: 48 GAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK----------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSP-----KTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGSEVSDYEPEAKIAKNMAQNDIFNSSDDSD-----------APK-------VKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKK--KVLDSDDDAPIKKPKAKKPKASPKVKKPPAAATKKSKK 1499
G KRLSVERIYQKK+QLEHILLRPDTYIGSV VT++MW+ DE+ I +E+T+VPGLYKIFDEILVNAADNKQRD M I++ + R N I ++NNG+GIPV HK EKM+VP LIFG LLTSSN++D+E+KVTGGRNGYGAKLCN+FSTKFTVETASRE +K FKQ W +NM +A + +LK FS G DYT ITF PDL KF M+SLD D VAL+ RRAYDIA S+K VKVFLNG R+ VK F+ YVD +LK DETG+ +K ++E RWEV + +S+ GFQQ+SFVNSIAT+KGGRHVDYV+ I S + +V+KKKNKGGV +K Q++NH+W+F N +I NPTFDSQTKENMTL FGS C + KF G+VES+L W KFKA ++ K S+ K N++KGI KL+DANDAG++NS +CTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREA+HKQI+EN EIN ++KI+GL YKK Y D LK+LRYGK+MIMTDQDQDGSHIKGLLINFIHHNWPSLLR FLEEFITPIVK +K +F+SLPE EWKS N +K+KYYKGLGTSTSKEAKEYF++MKRH+I FK+ +DD AI++AFSKK +D RKEWLT +ME+ ++RK LGL E YLY + T ++Y+DFINKEL+LFSN DNERSIPS+VDG KPGQRKV+FTC KRNDKREVKVAQLAGSV EMS+YHHGE SLM TIINLAQNFVGSNN+NLL PIGQFGTRL GGKD ASPRYIFT +SPL RL+F S DD L +L DDNQ++EPEWY+PIIPMVL+NGA+GIGTGW KIPN++ RE+V N+ ++DG EP M P +K++KG IE + + +YV+NGE+A ++ IE+TELP+R+WTQ+YKEQV+EPMLNG+EK P +I DY+EYHTD TVKFV++M+ +KL +AE GLH+ FKLQT+++ +SMVLFD GCL+KY+ VL+I+R+FF LRL +Y RK +L GML AES KL+NQARFILEK DG +V+ENK +K LI+ L ++G+DSDPVK WK AQ+ EE + E ++YLL M +W LT+E+KDEL +++DEK QEL L+ + DLW+ DL F+E+L+ VEAKE +DE+V L + K K ++ E LPSPVG RV+P + E++ +A K K R+ K+ +N++ A + L ++L K++ + T K+ + P K + + PW D SESD S +FD + EP+ + +AK ++ +D F+ D+ D PK K+A P +D + D V P++P A ++D +E S S KRTI+K + + A K+ K+ + KKP A K S+K
Sbjct: 23 GKKRLSVERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDED-VGINYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTMMRN-NLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFS-GEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNRLPVKGFRSYVDMYLKDKVDETGNALKVVHEQVNPRWEVCLTMSEKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKADQVKNHMWIFGNAVIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSAECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSTNPNHKKWKVKYYKGLGTSTSKEAKEYFANMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTMYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPSKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNYKGTIEELASNQYVINGEVAILNSTTIEITELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMTEEKLAEAE-RVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPEEEENEENEESESESTSPAAESGPTFNYLLDMPLWYLTKEKKDELCKQRDEKEQELNTLKKKTPSDLWKEDLAAFVEELEVVEAKEKQDEQVGLPGKGVKAKGKKAQIS-EVLPSPVGKRVIPQVTMEMRAEAEK----KIRR--KIKSENVEGTPA----------EDGAEPGLRQRL--------EKRQKREPGTRAKKQTTLPFKPIKKAQKQNPWSD----SESDMSSNESNFDVPPRE-----KEPRIAAT------KAKFTADLDSDDDFSGLDEKDEDEDFFPLDDTPPKTKMPPKNTKKALKPQKSSTSVDLESDGKDSV--PASPGAS--AADVPAETEPSKPSSKQTVGVKRTITKGQSLTSTAGTKKRAVPKETKSDSALNAHVSKKPAPAKAKNSRK 1461
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1215154270|gb|ASK50299.1| (DNA topoisomerase 2 [Eyprepocnemis plorans]) HSP 1 Score: 1768.05 bits (4578), Expect = 0.000e+0 Identity = 836/1245 (67.15%), Postives = 1013/1245 (81.37%), Query Frame = 0
Query: 35 ANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK----MEEDSDS-DSEGGLEKDLD--YDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEA-------LPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKK 1265
A G+A P A K ++VE+IYQKKSQLEHILLRPDTYIGSV PVTE MW+ D E + +V +EITYVPGLYKIFDEILVNAADNKQRD MD IKIDI+ E N + V+NNG+GIPV HKDEKM+VPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FSTKFTVETAS+E ++ FKQ W NNMSK + K+KDF+ G DYT++TF+PDL KF ME+LD D V+L+SRRAYD+AAS++GVKV LNGKR+ VK FKDYV+ ++K EDETG+P+K +YE +RWEVA+ +SD GFQQ+SFVNSIATTKGGRHVD+VS MI + E LKKKNK G+ IK Q++NH+WLF+NCLIVNPTFDSQTKENMTL FGSKC + KF + KSG+VES+L W+K KAD + + KK ++L GI KLEDAN AGT++S+DCTLILTEGDSAK+LAV+GLGV+GRDK+GV+PLRGKLLNVREA+HKQI++N EIN +++I+GL YKKKY +V+DLK+LRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLL+LPFLEEFITPIVKA+KG+ SF+SLPE EWK+ DNWHTYKIKYYKGLGTSTSKEAKEYF DMKRH+I F + DD INMAFSKK I+QRKEWLT+WM++ KRRKE+GL E YLYEKDT +V+Y+DF+NKELVLFSN DNERSIP +VDGFKPGQRKV+FTCLKRNDKREVKVAQLAGSV E SAYHHGE SLM TIINLAQN+VGSNNIN+L PIGQFGTRL GGKD ASPRYIFT++SPL R IF+ +DDA+L + TDDNQ+IEP WY+P+IPMVLVNGA+GIGTGWMTKIPNYNPREI+ NL MIDG P+ M PW+K++KG IE + +Y+V GEIA + E+K+E+TELPV WTQ+YKE V+EPML G+EK+P I ++EY+TD TV+FVV+M KL++AE +GLH+FFKL+T++S +SM FD CL++Y++V+EI++EFF+LRLN+Y KRK YLEGML+AESL+LSNQARFILEKCDG LVVENKK+K +IEEL+ +G+D DPV++WK Q +EE +++ DS+ +D+D YDYLLGM MW+LT+ERKDELL ++DEK QE +L+G S DLW+ DL +E+LD+VEAKE EDE + + KG K K+ A LPSP G RV P + + KA K A K KG++ KK
Sbjct: 2 AGGDA--PPAPKKNVKNMTVEKIYQKKSQLEHILLRPDTYIGSVEPVTELMWVYDPEQEMMVQREITYVPGLYKIFDEILVNAADNKQRDPKMDCIKIDIDAENNTVSVWNNGKGIPVVEHKDEKMYVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTKFTVETASKEYKRAFKQTWANNMSKTSDPKIKDFA-GEDYTKVTFSPDLTKFKMEALDKDIVSLMSRRAYDVAASTRGVKVMLNGKRLPVKTFKDYVELYIKNKEDETGNPLKIVYEQVNDRWEVALTISDKGFQQVSFVNSIATTKGGRHVDHVSDMIVKQLIETLKKKNKNGIQIKAFQVKNHMWLFVNCLIVNPTFDSQTKENMTLQAKNFGSKCPLSEKFINNVTKSGIVESILMWAKAKADSLLTKACNKKKQSRLTGIPKLEDANHAGTQHSLDCTLILTEGDSAKSLAVSGLGVIGRDKYGVFPLRGKLLNVREASHKQIMDNAEINNIIRIVGLQYKKKYESVNDLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLQLPFLEEFITPIVKASKGNQEFSFYSLPEFEEWKASKDNWHTYKIKYYKGLGTSTSKEAKEYFMDMKRHRIKFNYGGPNDDQCINMAFSKKCIEQRKEWLTSWMQDSKRRKEMGLPEQYLYEKDTHSVNYADFVNKELVLFSNCDNERSIPCMVDGFKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEHSAYHHGEQSLMSTIINLAQNYVGSNNINVLQPIGQFGTRLNGGKDSASPRYIFTKLSPLARFIFHKHDDALLKHNTDDNQRIEPVWYIPVIPMVLVNGANGIGTGWMTKIPNYNPREIIDNLRRMIDGEAPKQMNPWYKNYKGVIEPFGDSKYIVYGEIAVLGEDKVEITELPVGVWTQTYKENVLEPMLGGTEKVPQQIVQFQEYNTDTTVRFVVKMDPAKLQKAE-QEGLHKFFKLETSMSLNSMCAFDDMQCLKRYDSVIEILKEFFNLRLNYYGKRKLYLEGMLQAESLRLSNQARFILEKCDGVLVVENKKKKVIIEELKTRGYDPDPVRQWKLKQDKEALLEESNNADDSQDEATEDIDKLYDYLLGMTMWTLTKERKDELLSKRDEKLQEYEILKGKSPADLWKEDLDALLEELDKVEAKEREDEASGVVGKPVKGKKAAGSTKLAAISQKPVTLPSPGGRRVAPKLDPDRIKKAEKTTAVKENKGKRQVKK 1242
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1339043062|ref|XP_023709584.1| (DNA topoisomerase 2 isoform X2 [Cryptotermes secundus] >gi|1330910156|gb|PNF31624.1| DNA topoisomerase 2 [Cryptotermes secundus]) HSP 1 Score: 1756.88 bits (4549), Expect = 0.000e+0 Identity = 861/1351 (63.73%), Postives = 1063/1351 (78.68%), Query Frame = 0
Query: 36 NGEAKKPKAGGGGA------KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGS-EKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEED--SDSDSEG-----GLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILS-KESQKGAKGKKKLKIEAL------PSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS 1365
NG+ + +GG A K+L+VE+IYQKKSQLEHILLRPDTYIGSV PV E MW+ DEE +V KEI+YVPGLYKIFDEILVNAADNKQRD MD IKI+IN EKN I ++NNG+GIPV HKDEKMFVPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FST F VETA++E ++ FKQVW +NMSK E ++KD +G D+T+ITF+PDL KF ME+LD D VAL+S+RA+D+AAS++GVKV+LNGKR+ VK+FKDYV+ +LKG ED+ G+P+K +YE RWEVAV++S+ GFQQ+SFVNSIATTKGGRHVD++++ I + E LKKKNKGG+ IKPHQ++ HLW+F+NCLIVNPTFDSQTKE MTL FGSKC+ + KF + KS +VE+VL W+K KA+++++ + SSKK NKLKG+ KLEDANDAG++NS+DCTLILTEGDSAKTL V+GLGVVGRDK+GV+PLRGKLLNVREATHKQI EN+EI ++KI+GL YK KY ++DDLK+LRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLL+LPFLEEFITPIVKATKGS SF+SLPE EWK+ T+NWHTYKIKYYKGLGTSTSKEAKEYFSDM+RH+I FK+ DDH INMAFSKK +QRKEWLT+WME+ KRR+ELGL E YLYEKDTK+V+YSDF+NKELVLFSN DNERSIPS+VDG KPGQRKV+FTCLKR DKRE+KVAQLAGSV E S+YHHGE SLM TI+NLAQNFVGSNNINLL PIGQFGTRL+GGKD ASPRYIFT +SPLT IF+ +D +L + DD+QKIEP WY+PIIPMVLVNGADGIGTGWMTKIPNYNPREIV+NL M+D EPE M PW+K+F G +E + ++RY++ GEIA I E+K+E+TELP+ +WTQ+YKE V+EP+L+G+ EK P I DYKEY+TD TVKFVV M+ +K R AE + GLH+FFKLQTT+S SSM +FD C+RK+E+V IM+EFF +RL +YEKRK Y EGML+AES +LSNQARFI+EKCDG+LV+ENKK K +IEEL+R G+D DPV WK Q E + D+EG G + Y+YLL MA+++LT+E+K++LLR++D+K +L++L+ S LW+ DL F+E+LD+VEAKE EDE + S K ++ G K K E L PSP G RV+P I +LK K K +K KG++ K+ L E KDEFD M ++ ++L+++LG S D K +++P+ ++ K E+D+ VE D + S D+ S+ P++ S
Sbjct: 7 NGQVPEMMSGGDVAPKKPPNKKLTVEKIYQKKSQLEHILLRPDTYIGSVEPVVETMWLYDEEQDAMVQKEISYVPGLYKIFDEILVNAADNKQRDPKMDCIKIEINPEKNIISIWNNGQGIPVVEHKDEKMFVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTMFIVETAAKEYKRSFKQVWGSNMSKTSEPRIKD-HTGEDFTKITFSPDLSKFRMETLDKDIVALMSKRAFDVAASTRGVKVYLNGKRLPVKSFKDYVELYLKGKEDDQGNPLKCVYENVNPRWEVAVSLSEKGFQQVSFVNSIATTKGGRHVDHIAEHIVKQLIETLKKKNKGGLQIKPHQVKTHLWVFVNCLIVNPTFDSQTKETMTLQAKNFGSKCNLSDKFITNVTKSSIVEAVLTWAKSKAEKDLQKQCSSKKQNKLKGVPKLEDANDAGSRNSLDCTLILTEGDSAKTLVVSGLGVVGRDKYGVFPLRGKLLNVREATHKQIKENQEIGNVIKILGLQYKMKYESIDDLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLKLPFLEEFITPIVKATKGSTELSFYSLPEFEEWKASTNNWHTYKIKYYKGLGTSTSKEAKEYFSDMQRHRIKFKYGGPNDDHCINMAFSKKCAEQRKEWLTSWMEDTKRRRELGLPEQYLYEKDTKSVNYSDFVNKELVLFSNCDNERSIPSLVDGLKPGQRKVLFTCLKRADKREIKVAQLAGSVAEHSSYHHGEQSLMSTIVNLAQNFVGSNNINLLQPIGQFGTRLSGGKDSASPRYIFTLLSPLTSFIFHPHDMPLLKHNYDDSQKIEPVWYIPIIPMVLVNGADGIGTGWMTKIPNYNPREIVKNLKLMLDDQEPEPMVPWWKNFTGVVEPLVDKRYIIYGEIAVIGEDKLEITELPIGTWTQTYKESVLEPLLHGANEKTPPSITDYKEYNTDTTVKFVVTMNPEKRRAAEAE-GLHKFFKLQTTVSLSSMCVFDENCCMRKFESVELIMKEFFKVRLAYYEKRKKYHEGMLQAESRRLSNQARFIIEKCDGTLVIENKKMKVMIEELKRNGYDPDPVAVWKMKQNKAEALMENEDAEGSQLDDGSDVGGGYNYLLAMALYTLTEEKKEDLLRKRDDKLTQLKILQTKSPAALWKEDLDVFLEQLDKVEAKEKEDELGVSSGKTTKVGKKFGGSKKKEMLTAAETKPSPQGRRVIPKIDVKLKEKFEKETLSKENKGKRARKEKLLIEE--KDEFDMMDGNQ---KTLADRLGTSPEDIEKKMNAKRKEVKQRKPRKESSSRSPKKKGKGKNPWETDSEVE--DEELSSEDDGVSRPPPRTTS 1348
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1339043058|ref|XP_023709582.1| (DNA topoisomerase 2 isoform X1 [Cryptotermes secundus] >gi|1339043060|ref|XP_023709583.1| DNA topoisomerase 2 isoform X1 [Cryptotermes secundus]) HSP 1 Score: 1754.96 bits (4544), Expect = 0.000e+0 Identity = 861/1355 (63.54%), Postives = 1063/1355 (78.45%), Query Frame = 0
Query: 36 NGEAKKPKAGGGGA------KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGS-EKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEED------SDSDSEG-----GLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILS-KESQKGAKGKKKLKIEAL------PSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS 1365
NG+ + +GG A K+L+VE+IYQKKSQLEHILLRPDTYIGSV PV E MW+ DEE +V KEI+YVPGLYKIFDEILVNAADNKQRD MD IKI+IN EKN I ++NNG+GIPV HKDEKMFVPT+IFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FST F VETA++E ++ FKQVW +NMSK E ++KD +G D+T+ITF+PDL KF ME+LD D VAL+S+RA+D+AAS++GVKV+LNGKR+ VK+FKDYV+ +LKG ED+ G+P+K +YE RWEVAV++S+ GFQQ+SFVNSIATTKGGRHVD++++ I + E LKKKNKGG+ IKPHQ++ HLW+F+NCLIVNPTFDSQTKE MTL FGSKC+ + KF + KS +VE+VL W+K KA+++++ + SSKK NKLKG+ KLEDANDAG++NS+DCTLILTEGDSAKTL V+GLGVVGRDK+GV+PLRGKLLNVREATHKQI EN+EI ++KI+GL YK KY ++DDLK+LRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLL+LPFLEEFITPIVKATKGS SF+SLPE EWK+ T+NWHTYKIKYYKGLGTSTSKEAKEYFSDM+RH+I FK+ DDH INMAFSKK +QRKEWLT+WME+ KRR+ELGL E YLYEKDTK+V+YSDF+NKELVLFSN DNERSIPS+VDG KPGQRKV+FTCLKR DKRE+KVAQLAGSV E S+YHHGE SLM TI+NLAQNFVGSNNINLL PIGQFGTRL+GGKD ASPRYIFT +SPLT IF+ +D +L + DD+QKIEP WY+PIIPMVLVNGADGIGTGWMTKIPNYNPREIV+NL M+D EPE M PW+K+F G +E + ++RY++ GEIA I E+K+E+TELP+ +WTQ+YKE V+EP+L+G+ EK P I DYKEY+TD TVKFVV M+ +K R AE + GLH+FFKLQTT+S SSM +FD C+RK+E+V IM+EFF +RL +YEKRK Y EGML+AES +LSNQARFI+EKCDG+LV+ENKK K +IEEL+R G+D DPV WK Q E + D+EG G + Y+YLL MA+++LT+E+K++LLR++D+K +L++L+ S LW+ DL F+E+LD+VEAKE EDE + S K ++ G K K E L PSP G RV+P I +LK K K +K KG++ K+ L E KDEFD M ++ ++L+++LG S D K +++P+ ++ K E+D+ VE D + S D+ S+ P++ S
Sbjct: 7 NGQVPEMMSGGDVAPKKPPNKKLTVEKIYQKKSQLEHILLRPDTYIGSVEPVVETMWLYDEEQDAMVQKEISYVPGLYKIFDEILVNAADNKQRDPKMDCIKIEINPEKNIISIWNNGQGIPVVEHKDEKMFVPTMIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTMFIVETAAKEYKRSFKQVWGSNMSKTSEPRIKD-HTGEDFTKITFSPDLSKFRMETLDKDIVALMSKRAFDVAASTRGVKVYLNGKRLPVKSFKDYVELYLKGKEDDQGNPLKCVYENVNPRWEVAVSLSEKGFQQVSFVNSIATTKGGRHVDHIAEHIVKQLIETLKKKNKGGLQIKPHQVKTHLWVFVNCLIVNPTFDSQTKETMTLQAKNFGSKCNLSDKFITNVTKSSIVEAVLTWAKSKAEKDLQKQCSSKKQNKLKGVPKLEDANDAGSRNSLDCTLILTEGDSAKTLVVSGLGVVGRDKYGVFPLRGKLLNVREATHKQIKENQEIGNVIKILGLQYKMKYESIDDLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLKLPFLEEFITPIVKATKGSTELSFYSLPEFEEWKASTNNWHTYKIKYYKGLGTSTSKEAKEYFSDMQRHRIKFKYGGPNDDHCINMAFSKKCAEQRKEWLTSWMEDTKRRRELGLPEQYLYEKDTKSVNYSDFVNKELVLFSNCDNERSIPSLVDGLKPGQRKVLFTCLKRADKREIKVAQLAGSVAEHSSYHHGEQSLMSTIVNLAQNFVGSNNINLLQPIGQFGTRLSGGKDSASPRYIFTLLSPLTSFIFHPHDMPLLKHNYDDSQKIEPVWYIPIIPMVLVNGADGIGTGWMTKIPNYNPREIVKNLKLMLDDQEPEPMVPWWKNFTGVVEPLVDKRYIIYGEIAVIGEDKLEITELPIGTWTQTYKESVLEPLLHGANEKTPPSITDYKEYNTDTTVKFVVTMNPEKRRAAEAE-GLHKFFKLQTTVSLSSMCVFDENCCMRKFESVELIMKEFFKVRLAYYEKRKKYHEGMLQAESRRLSNQARFIIEKCDGTLVIENKKMKVMIEELKRNGYDPDPVAVWKMKQNKAEALENGMMENEDAEGSQLDDGSDVGGGYNYLLAMALYTLTEEKKEDLLRKRDDKLTQLKILQTKSPAALWKEDLDVFLEQLDKVEAKEKEDELGVSSGKTTKVGKKFGGSKKKEMLTAAETKPSPQGRRVIPKIDVKLKEKFEKETLSKENKGKRARKEKLLIEE--KDEFDMMDGNQ---KTLADRLGTSPEDIEKKMNAKRKEVKQRKPRKESSSRSPKKKGKGKNPWETDSEVE--DEELSSEDDGVSRPPPRTTS 1352
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|321457974|gb|EFX69050.1| (hypothetical protein DAPPUDRAFT_329527 [Daphnia pulex]) HSP 1 Score: 1746.48 bits (4522), Expect = 0.000e+0 Identity = 855/1279 (66.85%), Postives = 1035/1279 (80.92%), Query Frame = 0
Query: 35 ANGEAKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGS----LSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQK--------MEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKG---AKGKKKLKI-EALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVS 1297
NG+A K G KRLSVERIYQKKSQLEHILLRPDTYIGSV PV + MW+ EE+ ++ +EITYVPGLYKIFDEILVNAADNK RD MDTIK+DI+ ++N I ++NNG+GIPV HK+EKM+VP+LIFGHLLTSSN+NDEEEKVTGGRNGYGAKLCN+FST F +ETAS++ RK+FKQ W +NM+KA K+ +++ +D+T+ITF+PDL KF M+SLD DTVALLSRRA+D+AAS++GVKVFLNGKR+ +KNFKDYVD LKG ED+ G+ +K +YEA ERWE+AVA SD GFQQ+SF NSIATTKGGRHVDYV++++ + E LKKKNK G+ +KPHQI+NHLW+F+NCLIVNPTFDSQTKENMTL FGS C + KFF L KSGV+E V+ W+KFKA E+ S KK KLKG+ KLEDAN+AGTK+SIDCTLILTEGDSAK+LAV+GLGVVGRDK+GV+PL+GK+LNVREATHKQI+EN EIN ++KI+GL YKKKY +V+DLKSLRYG+LMIMTDQDQDGSHIKGLLINF+HHNWPSLLRLPFLEEFITPIVKATKG+ S SF+SLPE EWK++T+NW TY+IKYYKGLGTSTS+EAK YFSDM+RH+ILF H+ DQDD+AI MAFSKK ++QRKEWLT+WMEECKRR+ELGL E+YLYEKDTK VSY DF+NKELVLFSN+DNERSIPS++DGFKPGQRKV+FTC+KRNDK+E+KVAQLAGSV E SAYHHGEASLMGTII LAQ+FVGSNNINLLLP GQFGTRL GGKD ASPRYIFT MS LTR+IF+ +DD +L + DDNQ+IEPE+Y PIIPM+LVNG DGIGTGWMTKI NYNPRE+V N+ M+DG EP M PWFK ++G IE I+ RY ++GE+A +S ++E+TELP+R WTQ+YKE VME +L+GSEKIP +I DYKEYHTD TV+F+V MS +K++QAE + GLH+ FKLQTT S +SMVL+D+ GCL+ Y+ V +I++EFF +RL +Y KRK+YL G+L+AES +LSNQARFI EKCD L+VENKK K L+EEL+R+ +D DPV+ WK QK EE++++++E G K DYDYLL M M SLT E+K+ELL+++D K +E +L S DLWR DL FM L EVE + E E + +K+ K AKG+KK I E PSP G R+VP+I EL+ K K AK +K EK KK EVE +D FD DD N+SL EKLGVS
Sbjct: 25 TNGDAGKK---NGANKRLSVERIYQKKSQLEHILLRPDTYIGSVEPVKDVMWVY-EEDAGMIQREITYVPGLYKIFDEILVNAADNKVRDPKMDTIKVDIDPKENVITIYNNGKGIPVVEHKEEKMYVPSLIFGHLLTSSNYNDEEEKVTGGRNGYGAKLCNIFSTSFELETASKDERKKFKQTWTDNMTKAGTPKIVNYNE-ADFTQITFSPDLAKFKMDSLDKDTVALLSRRAFDVAASTRGVKVFLNGKRLPIKNFKDYVDLCLKGKEDDDGAQLKCVYEAVSERWEIAVAPSDKGFQQVSFANSIATTKGGRHVDYVTEIVSKYLQEFLKKKNKSGINVKPHQIKNHLWIFVNCLIVNPTFDSQTKENMTLQAKSFGSTCKPSEKFFNALNKSGVIEGVMTWAKFKAQTELNKACSGKKQTKLKGVPKLEDANNAGTKHSIDCTLILTEGDSAKSLAVSGLGVVGRDKYGVFPLKGKVLNVREATHKQILENAEINNLIKILGLQYKKKYESVEDLKSLRYGRLMIMTDQDQDGSHIKGLLINFLHHNWPSLLRLPFLEEFITPIVKATKGTGASAKSLSFYSLPEFEEWKTNTENWPTYRIKYYKGLGTSTSQEAKGYFSDMQRHRILFHHDGDQDDYAILMAFSKKHVEQRKEWLTSWMEECKRRRELGLPELYLYEKDTKRVSYRDFVNKELVLFSNMDNERSIPSLMDGFKPGQRKVLFTCIKRNDKKEIKVAQLAGSVAEHSAYHHGEASLMGTIIGLAQDFVGSNNINLLLPNGQFGTRLQGGKDAASPRYIFTLMSKLTRIIFHPHDDPLLKHQMDDNQRIEPEYYAPIIPMILVNGTDGIGTGWMTKILNYNPREVVANVKRMLDGEEPIPMDPWFKGYRGTIERIDRHRYAISGELAVLSGTEMEITELPLRVWTQNYKESVMETLLHGSEKIPPLITDYKEYHTDTTVRFIVTMSEEKMQQAEAE-GLHKAFKLQTTHSLTSMVLYDSNGCLKMYDTVEDILKEFFTVRLGYYGKRKDYLSGVLQAESQRLSNQARFICEKCDNVLIVENKKAKVLVEELRRRNYDPDPVRIWKKNQKNNVNAENEEEEEANAENEEGAVKIGDYDYLLDMPMRSLTYEKKEELLKKRDVKMKEYDILLKKSPADLWREDLDHFMVVLKEVE--DAEQEELNENKKPSKAAPLAKGRKKALIAEVAPSPKGRRIVPVIDNELRKKIEKLTIAKEKKKEKALKKESPEVE--RDSFDIALDDS--NKSLGEKLGVS 1291
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1325310473|ref|XP_023336845.1| (LOW QUALITY PROTEIN: DNA topoisomerase 2-alpha-like [Eurytemora affinis]) HSP 1 Score: 1739.16 bits (4503), Expect = 0.000e+0 Identity = 880/1302 (67.59%), Postives = 1049/1302 (80.57%), Query Frame = 0
Query: 39 AKKPKAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRN-DKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ--------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRR--KDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKKPW 1329
A KPK G KRLSVERIYQKKSQLEHILLRPDTYIGSV PVTE MW+ ++EN +VN+E+TYVPG YKI+DEILVNAADNKQRD MDTIKIDI E+ I + NNG+GIPV MHK+E MFVPT+IFGHLLTSSNFNDEEEKVTGGRNG+GAKLCNVFSTKFTVETA ++ K FKQ W +NMSKA E K+KDF+ G +YT+IT++PDL KFNME +D DT AL SRRA+DIAAS+KGVKVFLNGKR+ VK+FKDYVD ++KG EDE+G+ +K I+EA G RWEVAV VSD GFQQMSFVNSIATTKGGRHVD+V+ + I + +KK NK G+ IK Q++NHLWLF+NCLIVNPTFDSQTKE MTL + FGSKC + KF LQKSG++ESV+AWSKFKAD ++ +K KKT+KL+GI KLEDAN+AGTKNS CTLILTEGDSAKTLAVAGLGV+GRD +GVYPL+GKLLNVREATHKQI+ENKEIN++VKIIGL YKKKY T++D+KSLRYGK++I+ + D IKGL+INFIHHNWP LL+LPFLEEFITPIVK + SF+ K D W + ++ GLGTSTSKEAKEYFSDM RH+I FK+ QDDH+I +AFSKK I+ RKEWLTTWMEE KRRKELGL E+YLYEKDTK V+Y+DF+NKELVLFSN+DNERSIPS+VDGFKPGQRKV+FTCLKRN K+E+KVAQLAGSV E+SAYHHGEASLMGTIINLAQN+VGSNNINLL PIGQFGTRL GGKD ASPRYIFTQ+SPL R IFN DDA+LNY DDNQKIEPEWY+PI+PMVLVNGADGIGTGWMTKIPNYNPREIV+NL MIDG EP+ M PWFK FKG IE I++QRY V+GEI+++S+ K+E+TELP+R+WT SYKE ++E +LNG+EK+PA+I D+K+Y+TD TVKF++ M DKLR AE +KGLH FFKLQT+IS +SMVLFD GCLR++++V EI+REFF LRL FY KRK YLEGML AE+ KLSNQARFILEKCD +L +ENKK+K +IEELQR+G+DSDP+K WK +Q +E++ GG DYDYLLGM +W LTQE+KDELL++ + + + +L TSKE+LW+ DL F+ KLDE E E+ D+ +K +KG K LK+EALPSP+GIRV P IA+ELK K AKA AAK+RK +K DKK++K+ E KDEFD M +DK++N+SLSE++G +K K+ T +SSPK KGK PW
Sbjct: 16 APKPK----GDKRLSVERIYQKKSQLEHILLRPDTYIGSVQPVTEPMWVYEDEN--MVNREVTYVPGFYKIYDEILVNAADNKQRDPKMDTIKIDIKPEEGVISIMNNGKGIPVVMHKEENMFVPTMIFGHLLTSSNFNDEEEKVTGGRNGFGAKLCNVFSTKFTVETADKKAGKGFKQTWASNMSKAGEPKIKDFN-GEEYTKITYSPDLKKFNMEKIDDDTFALFSRRAFDIAASTKGVKVFLNGKRLPVKSFKDYVDQYIKGKEDESGNQIKCIHEACGPRWEVAVCVSDKGFQQMSFVNSIATTKGGRHVDHVADAVIKAIIDKIKKSNKTGINIKNFQVKNHLWLFVNCLIVNPTFDSQTKETMTLGVKDFGSKCVLSDKFQTSLQKSGIIESVVAWSKFKADMKLSSKAGGKKTSKLRGIAKLEDANEAGTKNSGGCTLILTEGDSAKTLAVAGLGVIGRDYYGVYPLKGKLLNVREATHKQILENKEINDLVKIIGLAYKKKYETMEDIKSLRYGKVLILLKLEFDTPQIKGLVINFIHHNWPGLLKLPFLEEFITPIVKVSHYRSLMSFYH-------KVGYDFW--VQFIHFIGLGTSTSKEAKEYFSDMVRHRIKFKYNGSQDDHSITLAFSKKEIEGRKEWLTTWMEEGKRRKELGLPEVYLYEKDTKAVNYTDFVNKELVLFSNMDNERSIPSLVDGFKPGQRKVVFTCLKRNLTKKEIKVAQLAGSVAEISAYHHGEASLMGTIINLAQNYVGSNNINLLQPIGQFGTRLQGGKDSASPRYIFTQLSPLARHIFNEADDALLNYQLDDNQKIEPEWYIPILPMVLVNGADGIGTGWMTKIPNYNPREIVKNLKLMIDGKEPKPMIPWFKGFKGTIEEIDSQRYAVSGEISTLSDTKVEITELPIRTWTNSYKE-LLEGLLNGTEKLPAVISDFKDYNTDRTVKFIITMDKDKLRNAEREKGLHAFFKLQTSISNTSMVLFDHLGCLRRFDSVEEILREFFTLRLEFYGKRKRYLEGMLGAEAAKLSNQARFILEKCDNTLTIENKKKKVMIEELQRRGYDSDPIKAWKKSQDNMDEEAAGEDEEAAESPAGG----PDYDYLLGMKLWCLTQEKKDELLKKLWRCXRINXIYILXATSKEELWKVDLDAFLAKLDETEDAELADD-AGAAKLGKKGPGKKGALKLEALPSPMGIRVEPRIADELKVKVAKAVAAKARKADKGDKKSVKK-EDDKDEFDLMTEDKELNKSLSERIGAVKKEKPLKQ------TKLNFEKSSPKKKGKNPW 1288
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1316162989|ref|XP_023226762.1| (DNA topoisomerase 2-alpha-like isoform X1 [Centruroides sculpturatus] >gi|1316162991|ref|XP_023226763.1| DNA topoisomerase 2-alpha-like isoform X2 [Centruroides sculpturatus]) HSP 1 Score: 1736.08 bits (4495), Expect = 0.000e+0 Identity = 843/1317 (64.01%), Postives = 1043/1317 (79.20%), Query Frame = 0
Query: 50 KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGGLEKDL---DYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKES---QKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAK----AAAAKSR-KGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPKKRPQSSPKTKGKK------PWEDSDATSESDNSVEFDDFDD 1349
KRLSVERIYQKK+QLEHILLRPDTYIGSV PVT+ MW+ D ++ +++++VPGLYKIFDEILVNAADNKQRDK+MD IK+DI+ E N+I ++NNG+GIPV HK EK+FVP+LIFGHLLTSSN+NDEE+KVTGGRNGYGAKLCN+FSTKF VET+S+E +K FKQ W +NMSK EA++ + G D+T +TF+PDL KF ME LD DTVAL+SRR YD+A SS+GVKV+LNGKR+ ++ FK+YV+ ++K DE G+P+K +YE+ +RWEVAV +S+ GFQQ+SFVNSIATTKGGRHVDYV++ I S +A+V+KKKNKGGV IKP QI+NH+W+F+NCLI NPTFDSQTKENMTL FGSKC + KFF Q+ K G++E +++W +FKA E+ K S+ K +KL+GI KLEDANDAGTK SIDCTLILTEGDSAKTLAV+GLGVVGRDK+GV+PLRGK+LNVREATHKQI+EN+EIN ++KI+GL YKKKY ++DDLK+LRYG+LMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVK +KG SF+SLPE EWK T NW+T+KIKYYKGLGTSTSKEAKEYF+DM RH+I FK+E QDD+AI +AFSKK +DQRKEWLT MEE K R+ELGL E+YLY K+T ++++DF+NKEL+LFSN+DNERSIPS+VDG KPGQRKVM+TC KRNDKRE+KVAQLAGSV E SAYHHGE++LMGTIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT +SPL +LIF+ D+ +L +L DDNQ+IEPE+Y+P+IPMVLVNG +GIGTGW TKIPNYNPREIV N+ M+ G EP M PWFK+FKG IE ++NQRYV+NGE++ +S NK+E+TELPVR+WTQ+YKEQV+E ML+GS+K+P++I DYKEYHTD TV+FVV M+ +KL ++E ++GLH+ FKLQTT+STSSMVLFD KGCLR+Y++ EI++EFF +RL+ YE+RK YLEGML AES KL +QARFILEK +G +++EN+K+ LI LQ +G++SDPVK WK +Q D++ +E E+D DYDYLLGM +WSLT+ERKDELL+++D+K EL LR + DLW DL M +LD VE KE EDE + +K++ K KG+K E PSP+G RV+P I +LK +A K K R KG+K D + +KE +C++ + E LG S KK+ SST K + S KG K PW +ES+NS D DD
Sbjct: 30 KRLSVERIYQKKTQLEHILLRPDTYIGSVEPVTQSMWVYDGPETGMIFRDVSFVPGLYKIFDEILVNAADNKQRDKSMDCIKVDIDPENNKISIWNNGKGIPVVEHKVEKIFVPSLIFGHLLTSSNYNDEEKKVTGGRNGYGAKLCNIFSTKFVVETSSKEYKKSFKQCWTDNMSKTTEARIIP-NKGEDFTCVTFSPDLSKFGMEVLDKDTVALMSRRVYDVAGSSRGVKVYLNGKRLPIRGFKEYVEQYIKDKNDEQGNPLKVVYESVNDRWEVAVTMSERGFQQVSFVNSIATTKGGRHVDYVTEQIVSKLADVVKKKNKGGVQIKPFQIKNHMWVFVNCLIENPTFDSQTKENMTLQAKNFGSKCPLSDKFFNQVIKCGIIECIMSWVRFKAQTELNKKCSATKHSKLRGIPKLEDANDAGTKGSIDCTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKMLNVREATHKQILENQEINNIIKIMGLQYKKKYESIDDLKTLRYGRLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKISKGKEEMSFYSLPEYEEWKQQTPNWNTWKIKYYKGLGTSTSKEAKEYFADMNRHRIKFKYEGTQDDNAIQLAFSKKLVDQRKEWLTNGMEERKHRRELGLPELYLYGKNTTNITFNDFVNKELILFSNMDNERSIPSLVDGLKPGQRKVMYTCFKRNDKREIKVAQLAGSVAEHSAYHHGESALMGTIINLAQNFVGSNNINLLQPIGQFGTRLQGGKDAASPRYIFTMLSPLAKLIFSQLDEPLLVHLFDDNQRIEPEYYIPVIPMVLVNGCEGIGTGWSTKIPNYNPREIVSNIRRMLKGEEPVKMNPWFKNFKGVIEQLDNQRYVINGEVSILSSNKLEITELPVRTWTQTYKEQVLETMLHGSDKVPSLITDYKEYHTDSTVRFVVTMNEEKLSKSE-EQGLHKVFKLQTTLSTSSMVLFDHKGCLRRYDSPEEILKEFFEVRLSCYERRKAYLEGMLTAESKKLDSQARFILEKIEGKIIIENRKKSELIRTLQERGYESDPVKSWKESQNKNTDNEETNEDDEERDSSGPDYDYLLGMNLWSLTRERKDELLKKRDDKRAELEALREKTPADLWEDDLTNLMVELDRVEEKEREDEIIGGTKKAIKLGKSGKGRKVALEETKPSPMGQRVIPHIDPDLKKRAEKTPLGTGEGKRRGKGKKTDSELIKE----------LCENGE------EGLGNSDKSNKMKKKLKCSSTLLKAVKKSGSPKGNKKGPKRNPWSSESDGNESNNS----DVDD 1324
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1067070039|ref|XP_018018369.1| (PREDICTED: DNA topoisomerase 2-alpha-like isoform X3 [Hyalella azteca]) HSP 1 Score: 1734.15 bits (4490), Expect = 0.000e+0 Identity = 881/1489 (59.17%), Postives = 1109/1489 (74.48%), Query Frame = 0
Query: 43 KAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ-------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVI---LSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPK-----KRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS--------EVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKKPPAAATKKSKKTSQGSDYDD 1508
+A K+ +VE+ YQKK+QLEHI+LRPDTYIGSV VTE+MW+ D +++ V +EITYVPGLYKIFDEILVNAADNKQRD MDTIKI+I+ E N I V+NNG GIPV MHKDEKMFVPT+IFGHLLTSSN+ND+E+KVTGGRNG+GAKLCN+FSTKFTVETA++E +K FKQ W NMSKA + K+KDF+ G +YT+ITF+PDL KF M+ LD DTV LLSRRAYD+AASS GVKV+LNGKR+ VK+FKDYV+ ++K D+ G+P+K +YE G RWEVAV +S+ GFQ MSFVNSIATTKGGRHVDY++ MI +++ E L KKNK GV IK QI+NHLW+F+N LI NPTFDSQTKENMTL FGSKC+ + KF + KSG+VE+V+AW+KFK +++ K + KKT+KLKGI KLEDANDAGT++S +CTLILTEGDSAKTLAVAGLGVVGRDK+GV+PLRGK+LN REAT KQ++EN EIN +++I+GL YKKKY TVDDLK+LRYGKLMIMTDQDQDGSHIKGL+INFIHHNWPSLLRL FLEEFITPIVKATKG SF+SLPE EWKS+ DNWHTYKIKYYKGLGTSTSKEAKEYFSDM RH+I FK+E DQDD +I MAFSKKA DQRKEWLT WM ECKRR+ELGL EIYLYEK+T +V+YSDF+NKELVLFSN DNERSIPS+VDGFKPGQRKVMFTC+KRNDKREVKVAQLAGS+ E +AYHHGE SLM TIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT++SPL R + + +DD +L YL +DNQKIEPEWYMPIIP+VLVNGA+GIGTGW TKIPNYNPREIV N+ MI G EP+DM PWFK+F+G +E ++ RY+ +GE+A + +N+ E++ELP+R+WTQSYKE V+E +L G++KIP II DY+EY+TD TV+FV+ + +++R E + G H+ FKLQ+++S ++MVLFDA G L++Y +V+++++EF+ LRL FYEKRK Y+ GML+AE+ KLSNQARFI EKC+G LVVENKKRK +I EL KG++SDPVK WK +Q + E + D+DYLLGM MWSLT+ERK++LL+++ EK EL L+ S+ DLW DL F+E+LD+VEAKE E+ERV L+ + +K KKK +E +PSP+G R+ P I E++ KA K+AAA + K + K++ + + + + +SL+ +LG S + KKR + T K K+ + + KGK PW SD+ ++ NS DS ++ P+S + + Y + + +S+D D +R P F LD ++++ P AP+ +P++ + S++P KK S + D+D + + K KKP A AA+TK++ T+ GS+ D
Sbjct: 29 QAAEPAGKKRTVEKTYQKKTQLEHIILRPDTYIGSVEKVTEQMWVFDTADEKFVQREITYVPGLYKIFDEILVNAADNKQRDPKMDTIKIEIDAENNVISVYNNGHGIPVVMHKDEKMFVPTMIFGHLLTSSNYNDDEKKVTGGRNGFGAKLCNIFSTKFTVETANKEYKKNFKQTWAVNMSKASDPKIKDFT-GEEYTKITFSPDLAKFGMDCLDRDTVDLLSRRAYDVAASSTGVKVYLNGKRVPVKSFKDYVELYIKDRTDDAGAPLKVVYEKPGPRWEVAVTISEKGFQHMSFVNSIATTKGGRHVDYITNMIVNSLTETLSKKNKSGVQIKNFQIKNHLWVFVNALIENPTFDSQTKENMTLQQKSFGSKCTLSDKFVAAVVKSGIVETVMAWAKFKQQAQLEKKCAGKKTSKLKGIPKLEDANDAGTRSSSECTLILTEGDSAKTLAVAGLGVVGRDKYGVFPLRGKVLNTREATVKQMMENAEINHIIQIVGLQYKKKYETVDDLKTLRYGKLMIMTDQDQDGSHIKGLIINFIHHNWPSLLRLNFLEEFITPIVKATKGKEEISFYSLPEYEEWKSNKDNWHTYKIKYYKGLGTSTSKEAKEYFSDMVRHRIKFKYEGDQDDRSIQMAFSKKAADQRKEWLTAWMLECKRRRELGLPEIYLYEKNTHSVNYSDFVNKELVLFSNADNERSIPSLVDGFKPGQRKVMFTCIKRNDKREVKVAQLAGSIAEHTAYHHGEVSLMSTIINLAQNFVGSNNINLLEPIGQFGTRLMGGKDAASPRYIFTKLSPLARALIHPHDDPLLKYLQEDNQKIEPEWYMPIIPLVLVNGAEGIGTGWSTKIPNYNPREIVDNIRKMIRGEEPKDMLPWFKNFRGTVEQLDATRYLCSGEVARLEDNQYEISELPIRTWTQSYKENVLE-ILQGNDKIPPIISDYREYNTDTTVRFVLNVLDNQVRHVEAE-GPHKIFKLQSSMSINTMVLFDANGVLKRYNSVMDVLQEFYELRLKFYEKRKLYMVGMLKAEANKLSNQARFIKEKCEGKLVVENKKRKDMINELITKGYESDPVKAWKKSQSREAAVEDEAAEESDAEEEQTDDAADFDYLLGMPMWSLTKERKEDLLKKEQEKKHELETLQAKSESDLWNHDLDVFIEELDKVEAKEREEERVAKENLASKDKKAGGKKKKALLEVMPSPMGERIQPRIDAEMRKKAEKSAAAGAAKAGRPKKRDNPDDDHDDLD---DLVSANTGKSLASRLGTSPD--LVKKRKAGRPTKKNGDGLKQTKINFPKKGKNPWS-SDSDADGGNS-------DSDLSTTNIETVPRSRAVEGKRAAAAKAKYNFSDDDEDDDDDIESLHSNDGVD----ERHVGPEKLSF-LDQTEDN----AAPDAPSPPKPAAKK---------SNAPKPKKANAHSDEDIVISDTDSEFGDFQQKKKKPSA--------AASTKQAASTADGSNASD 1475
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1067070035|ref|XP_018018366.1| (PREDICTED: DNA topoisomerase 2-alpha-like isoform X1 [Hyalella azteca]) HSP 1 Score: 1731.84 bits (4484), Expect = 0.000e+0 Identity = 880/1496 (58.82%), Postives = 1107/1496 (74.00%), Query Frame = 0
Query: 43 KAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ-------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLK----------IEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPK-----KRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS--------EVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKKPPAAATKKSKKTSQGSDYDD 1508
+A K+ +VE+ YQKK+QLEHI+LRPDTYIGSV VTE+MW+ D +++ V +EITYVPGLYKIFDEILVNAADNKQRD MDTIKI+I+ E N I V+NNG GIPV MHKDEKMFVPT+IFGHLLTSSN+ND+E+KVTGGRNG+GAKLCN+FSTKFTVETA++E +K FKQ W NMSKA + K+KDF+ G +YT+ITF+PDL KF M+ LD DTV LLSRRAYD+AASS GVKV+LNGKR+ VK+FKDYV+ ++K D+ G+P+K +YE G RWEVAV +S+ GFQ MSFVNSIATTKGGRHVDY++ MI +++ E L KKNK GV IK QI+NHLW+F+N LI NPTFDSQTKENMTL FGSKC+ + KF + KSG+VE+V+AW+KFK +++ K + KKT+KLKGI KLEDANDAGT++S +CTLILTEGDSAKTLAVAGLGVVGRDK+GV+PLRGK+LN REAT KQ++EN EIN +++I+GL YKKKY TVDDLK+LRYGKLMIMTDQDQDGSHIKGL+INFIHHNWPSLLRL FLEEFITPIVKATKG SF+SLPE EWKS+ DNWHTYKIKYYKGLGTSTSKEAKEYFSDM RH+I FK+E DQDD +I MAFSKKA DQRKEWLT WM ECKRR+ELGL EIYLYEK+T +V+YSDF+NKELVLFSN DNERSIPS+VDGFKPGQRKVMFTC+KRNDKREVKVAQLAGS+ E +AYHHGE SLM TIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT++SPL R + + +DD +L YL +DNQKIEPEWYMPIIP+VLVNGA+GIGTGW TKIPNYNPREIV N+ MI G EP+DM PWFK+F+G +E ++ RY+ +GE+A + +N+ E++ELP+R+WTQSYKE V+E +L G++KIP II DY+EY+TD TV+FV+ + +++R E + G H+ FKLQ+++S ++MVLFDA G L++Y +V+++++EF+ LRL FYEKRK Y+ GML+AE+ KLSNQARFI EKC+G LVVENKKRK +I EL KG++SDPVK WK +Q + E + D+DYLLGM MWSLT+ERK++LL+++ EK EL L+ S+ DLW DL F+E+LD+VEAKE E+ERV +KE+ K K +E +PSP+G R+ P I E++ KA K+AAA + K + K++ + + + + +SL+ +LG S + KKR + T K K+ + + KGK PW SD+ ++ NS DS ++ P+S + + Y + + +S+D D +R P F LD ++++ P AP+ +P++ + S++P KK S + D+D + + K KKP A AA+TK++ T+ GS+ D
Sbjct: 29 QAAEPAGKKRTVEKTYQKKTQLEHIILRPDTYIGSVEKVTEQMWVFDTADEKFVQREITYVPGLYKIFDEILVNAADNKQRDPKMDTIKIEIDAENNVISVYNNGHGIPVVMHKDEKMFVPTMIFGHLLTSSNYNDDEKKVTGGRNGFGAKLCNIFSTKFTVETANKEYKKNFKQTWAVNMSKASDPKIKDFT-GEEYTKITFSPDLAKFGMDCLDRDTVDLLSRRAYDVAASSTGVKVYLNGKRVPVKSFKDYVELYIKDRTDDAGAPLKVVYEKPGPRWEVAVTISEKGFQHMSFVNSIATTKGGRHVDYITNMIVNSLTETLSKKNKSGVQIKNFQIKNHLWVFVNALIENPTFDSQTKENMTLQQKSFGSKCTLSDKFVAAVVKSGIVETVMAWAKFKQQAQLEKKCAGKKTSKLKGIPKLEDANDAGTRSSSECTLILTEGDSAKTLAVAGLGVVGRDKYGVFPLRGKVLNTREATVKQMMENAEINHIIQIVGLQYKKKYETVDDLKTLRYGKLMIMTDQDQDGSHIKGLIINFIHHNWPSLLRLNFLEEFITPIVKATKGKEEISFYSLPEYEEWKSNKDNWHTYKIKYYKGLGTSTSKEAKEYFSDMVRHRIKFKYEGDQDDRSIQMAFSKKAADQRKEWLTAWMLECKRRRELGLPEIYLYEKNTHSVNYSDFVNKELVLFSNADNERSIPSLVDGFKPGQRKVMFTCIKRNDKREVKVAQLAGSIAEHTAYHHGEVSLMSTIINLAQNFVGSNNINLLEPIGQFGTRLMGGKDAASPRYIFTKLSPLARALIHPHDDPLLKYLQEDNQKIEPEWYMPIIPLVLVNGAEGIGTGWSTKIPNYNPREIVDNIRKMIRGEEPKDMLPWFKNFRGTVEQLDATRYLCSGEVARLEDNQYEISELPIRTWTQSYKENVLE-ILQGNDKIPPIISDYREYNTDTTVRFVLNVLDNQVRHVEAE-GPHKIFKLQSSMSINTMVLFDANGVLKRYNSVMDVLQEFYELRLKFYEKRKLYMVGMLKAEANKLSNQARFIKEKCEGKLVVENKKRKDMINELITKGYESDPVKAWKKSQSREAAVEDEAAEESDAEEEQTDDAADFDYLLGMPMWSLTKERKEDLLKKEQEKKHELETLQAKSESDLWNHDLDVFIEELDKVEAKEREEERV--AKENLASKDKKAGGKKKKKYVDDALLEVMPSPMGERIQPRIDAEMRKKAEKSAAAGAAKAGRPKKRDNPDDDHDDLD---DLVSANTGKSLASRLGTSPD--LVKKRKAGRPTKKNGDGLKQTKINFPKKGKNPWS-SDSDADGGNS-------DSDLSTTNIETVPRSRAVEGKRAAAAKAKYNFSDDDEDDDDDIESLHSNDGVD----ERHVGPEKLSF-LDQTEDN----AAPDAPSPPKPAAKK---------SNAPKPKKANAHSDEDIVISDTDSEFGDFQQKKKKPSA--------AASTKQAASTADGSNASD 1480
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1067070037|ref|XP_018018367.1| (PREDICTED: DNA topoisomerase 2-alpha-like isoform X2 [Hyalella azteca]) HSP 1 Score: 1730.3 bits (4480), Expect = 0.000e+0 Identity = 882/1497 (58.92%), Postives = 1103/1497 (73.68%), Query Frame = 0
Query: 43 KAGGGGAKRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQ-------KMEEDSDSDSEGGLEKDLDYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLK----------IEALPSPVGIRVVPLIAEELKNKAAKAAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSVNDKGAKKRGSDSSTPK-----KRPQSSPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSDNKKSKSEPKSGS--------EVSDYEPEAKIAKNMAQNDIFNSSDDSDAPKVKRATAPAHKVFDLDDSDEDFDKVLKPSAPAAKRPSSDQKSEVVDSDDSDSPIKKKKRTISKAKKKVLDSDDDAPIKKPKAKKPKASPKVKKPPAAA-TKKSKKTSQGSDYDD 1508
+A K+ +VE+ YQKK+QLEHI+LRPDTYIGSV VTE+MW+ D +++ V +EITYVPGLYKIFDEILVNAADNKQRD MDTIKI+I+ E N I V+NNG GIPV MHKDEKMFVPT+IFGHLLTSSN+ND+E+KVTGGRNG+GAKLCN+FSTKFTVETA++E +K FKQ W NMSKA + K+KDF+ G +YT+ITF+PDL KF M+ LD DTV LLSRRAYD+AASS GVKV+LNGKR+ VK+FKDYV+ ++K D+ G+P+K +YE G RWEVAV +S+ GFQ MSFVNSIATTKGGRHVDY++ MI +++ E L KKNK GV IK QI+NHLW+F+N LI NPTFDSQTKENMTL FGSKC+ + KF + KSG+VE+V+AW+KFK +++ K + KKT+KLKGI KLEDANDAGT++S +CTLILTEGDSAKTLAVAGLGVVGRDK+GV+PLRGK+LN REAT KQ++EN EIN +++I+GL YKKKY TVDDLK+LRYGKLMIMTDQDQDGSHIKGL+INFIHHNWPSLLRL FLEEFITPIVKATKG SF+SLPE EWKS+ DNWHTYKIKYYKGLGTSTSKEAKEYFSDM RH+I FK+E DQDD +I MAFSKKA DQRKEWLT WM ECKRR+ELGL EIYLYEK+T +V+YSDF+NKELVLFSN DNERSIPS+VDGFKPGQRKVMFTC+KRNDKREVKVAQLAGS+ E +AYHHGE SLM TIINLAQNFVGSNNINLL PIGQFGTRL GGKD ASPRYIFT++SPL R + + +DD +L YL +DNQKIEPEWYMPIIP+VLVNGA+GIGTGW TKIPNYNPREIV N+ MI G EP+DM PWFK+F+G +E ++ RY+ +GE+A + +N+ E++ELP+R+WTQSYKE V+E +L G++KIP II DY+EY+TD TV+FV+ + +++R E + G H+ FKLQ+++S ++MVLFDA G L++Y +V+++++EF+ LRL FYEKRK Y+ GML+AE+ KLSNQARFI EKC+G LVVENKKRK +I EL KG++SDPVK WK +Q + E + D+DYLLGM MWSLT+ERK++LL+++ EK EL L+ S+ DLW DL F+E+LD+VEAKE E+ERV +KE+ K K +E +PSP+G R+ P I E++ KA K+AAA + K + K++ + + + + +SL+ +LG S + KKR + T K K+ + + KGK PW SD+ ++ NS DS ++ P+S + + Y + + +S+D D +R P F LD ++++ P AP+ +P+ KK K KK SD+D I ++ K KKP AAA TK++ T+ GS+ D
Sbjct: 29 QAAEPAGKKRTVEKTYQKKTQLEHIILRPDTYIGSVEKVTEQMWVFDTADEKFVQREITYVPGLYKIFDEILVNAADNKQRDPKMDTIKIEIDAENNVISVYNNGHGIPVVMHKDEKMFVPTMIFGHLLTSSNYNDDEKKVTGGRNGFGAKLCNIFSTKFTVETANKEYKKNFKQTWAVNMSKASDPKIKDFT-GEEYTKITFSPDLAKFGMDCLDRDTVDLLSRRAYDVAASSTGVKVYLNGKRVPVKSFKDYVELYIKDRTDDAGAPLKVVYEKPGPRWEVAVTISEKGFQHMSFVNSIATTKGGRHVDYITNMIVNSLTETLSKKNKSGVQIKNFQIKNHLWVFVNALIENPTFDSQTKENMTLQQKSFGSKCTLSDKFVAAVVKSGIVETVMAWAKFKQQAQLEKKCAGKKTSKLKGIPKLEDANDAGTRSSSECTLILTEGDSAKTLAVAGLGVVGRDKYGVFPLRGKVLNTREATVKQMMENAEINHIIQIVGLQYKKKYETVDDLKTLRYGKLMIMTDQDQDGSHIKGLIINFIHHNWPSLLRLNFLEEFITPIVKATKGKEEISFYSLPEYEEWKSNKDNWHTYKIKYYKGLGTSTSKEAKEYFSDMVRHRIKFKYEGDQDDRSIQMAFSKKAADQRKEWLTAWMLECKRRRELGLPEIYLYEKNTHSVNYSDFVNKELVLFSNADNERSIPSLVDGFKPGQRKVMFTCIKRNDKREVKVAQLAGSIAEHTAYHHGEVSLMSTIINLAQNFVGSNNINLLEPIGQFGTRLMGGKDAASPRYIFTKLSPLARALIHPHDDPLLKYLQEDNQKIEPEWYMPIIPLVLVNGAEGIGTGWSTKIPNYNPREIVDNIRKMIRGEEPKDMLPWFKNFRGTVEQLDATRYLCSGEVARLEDNQYEISELPIRTWTQSYKENVLE-ILQGNDKIPPIISDYREYNTDTTVRFVLNVLDNQVRHVEAE-GPHKIFKLQSSMSINTMVLFDANGVLKRYNSVMDVLQEFYELRLKFYEKRKLYMVGMLKAEANKLSNQARFIKEKCEGKLVVENKKRKDMINELITKGYESDPVKAWKKSQSREAAVEDEAAEESDAEEEQTDDAADFDYLLGMPMWSLTKERKEDLLKKEQEKKHELETLQAKSESDLWNHDLDVFIEELDKVEAKEREEERV--AKENLASKDKKAGGKKKKKYVDDALLEVMPSPMGERIQPRIDAEMRKKAEKSAAAGAAKAGRPKKRDNPDDDHDDLD---DLVSANTGKSLASRLGTSPD--LVKKRKAGRPTKKNGDGLKQTKINFPKKGKNPWS-SDSDADGGNS-------DSDLSTTNIETVPRSRAVEGKRAAAAKAKYNFSDDDEDDDDDIESLHSNDGVD----ERHVGPEKLSF-LDQTEDN----AAPDAPSPPKPA-------------------KKSNAPKPKKANAHSDEDIVISDTDSEFGDFQQKKKKPSAAASTKQAASTADGSNASD 1479
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Match: gi|1355946814|gb|PRD20904.1| (DNA topoisomerase 2-alpha [Nephila clavipes]) HSP 1 Score: 1711.43 bits (4431), Expect = 0.000e+0 Identity = 829/1314 (63.09%), Postives = 1038/1314 (79.00%), Query Frame = 0
Query: 50 KRLSVERIYQKKSQLEHILLRPDTYIGSVNPVTEKMWILDEENQRIVNKEITYVPGLYKIFDEILVNAADNKQRDKNMDTIKIDINREKNEIRVFNNGRGIPVEMHKDEKMFVPTLIFGHLLTSSNFNDEEEKVTGGRNGYGAKLCNVFSTKFTVETASRENRKEFKQVWENNMSKAKEAKLKDFSSGSDYTRITFTPDLPKFNMESLDADTVALLSRRAYDIAASSKGVKVFLNGKRIGVKNFKDYVDFFLKGHEDETGSPVKAIYEAAGERWEVAVAVSDNGFQQMSFVNSIATTKGGRHVDYVSKMIESNIAEVLKKKNKGGVTIKPHQIRNHLWLFINCLIVNPTFDSQTKENMTLLMTKFGSKCSFTPKFFGQLQKSGVVESVLAWSKFKADQEMKNKHSSKKTNKLKGILKLEDANDAGTKNSIDCTLILTEGDSAKTLAVAGLGVVGRDKFGVYPLRGKLLNVREATHKQIIENKEINEMVKIIGLTYKKKYSTVDDLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKATKGSLSTSFFSLPELHEWKSHTDNWHTYKIKYYKGLGTSTSKEAKEYFSDMKRHQILFKHESDQDDHAINMAFSKKAIDQRKEWLTTWMEECKRRKELGLSEIYLYEKDTKTVSYSDFINKELVLFSNLDNERSIPSVVDGFKPGQRKVMFTCLKRNDKREVKVAQLAGSVGEMSAYHHGEASLMGTIINLAQNFVGSNNINLLLPIGQFGTRLAGGKDHASPRYIFTQMSPLTRLIFNSNDDAVLNYLTDDNQKIEPEWYMPIIPMVLVNGADGIGTGWMTKIPNYNPREIVRNLLNMIDGAEPEDMKPWFKSFKGGIESIENQRYVVNGEIASISENKIEVTELPVRSWTQSYKEQVMEPMLNGSEKIPAIIQDYKEYHTDVTVKFVVQMSADKLRQAELDKGLHQFFKLQTTISTSSMVLFDAKGCLRKYENVLEIMREFFHLRLNFYEKRKNYLEGMLEAESLKLSNQARFILEKCDGSLVVENKKRKALIEELQRKGFDSDPVKKWKNAQKMEEDSDSDSEGGLEKDL-----DYDYLLGMAMWSLTQERKDELLRRKDEKHQELRMLRGTSKEDLWRTDLKEFMEKLDEVEAKEIEDERVILSKESQKGAKGKKKLKIEALPSPVGIRVVPLIAEELKNKAAK--AAAAKSRKGEKLDKKNLKEVEAVKDEFDFMCDDKDMNRSLSEKLGVSV--NDKGAKKRGSDSSTPKKRPQS-SPKTKGKKPWEDSDATSESDNSVEFDDFDDSGSD 1353
K+LSVERIYQKK+QLEHILLRPDTYIGSV PVT+ MW+ EEN +VN+E+TYVPGLYKIFDEILVNAADNKQRDK+MD I+I+IN EKN+I ++NNG+GIPV HKDEKMFVP+LIFGHLLTSSN+NDEE KVTGGRNGYGAKLCN+FSTKFTVETAS+ +K+FKQ W++NM+KA+E K+ F+ GSD+T ITF PDL KF M LD D VA+++RRAYDIA ++GVKV LN K++ VK+FKDY+ +L D+ G P+K +++ ERWE+AV +S++G+Q +SFVNSIATTKGGRHVDY++ + S IAEV+KKKNKGG +KP QI++HLW+F+NCLI NPTFDSQTKENMTL + FGSKC T KF Q K GVVES+++W +FKA E++ K S KK +KLKGI KLEDANDAGTKNSIDCTLILTEGDSAK+LAV+GLGVVGRDK+GV+PL+GK+LNVREA+HKQI+EN EIN ++KI+GL YKKKY ++DDLK+LRYG+LMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKA+KG F+SLPE EWK+ T NW+ +K+KYYKGLGTST+KEAKEYFSDMKRH+I F + ++DD+AI +AFSKK +D RKEWLT MEE KRR+ELGL E+YLY K+T V+Y+DF+NKEL+LFSN+DNERSIPS +DGFKPGQRKV+FTCLKRNDKREVKVAQL GSV E SAYHHGEA+L GTII LAQ+FVGSNNIN+LLP+GQFGTRL GGKD ASPRYIFT ++ +++ +F+ D +L+YL DDN KIEPE+Y+PIIPMVLVNGA+GIGTGW TK+PNYNPR++V+N+ ++DG +P M PW+K+F+G I+ +++Q ++V+GEIA + NKIE+TELP+R+WTQ YKE V+EPM++G+EKIPA I DYKEYHTD TV+FVV M+ +K QA ++G H+ FKLQT+IS SSMVLFD GCLRKY++ +++REF+ +R YE+RK Y+ G+LEAESLKL NQARFILEK + + +EN++R +I+ LQ +G+DSDPVK WK AQK D + SE EK++ DYDYLLGM +WSLT+ERKD+LL+++DEK +EL L+ S DLWR DLKEFME+LD+VE ++ EDE + S + +K GKKK E PSP G R+ P I ++ K K K + +KG K ++KE + D KD+ SLS++L V GAK + KKR SPK + PW D ++E + + D ++ G D
Sbjct: 24 KKLSVERIYQKKTQLEHILLRPDTYIGSVEPVTQTMWVY-EENVGLVNREVTYVPGLYKIFDEILVNAADNKQRDKSMDLIRIEINPEKNKICIWNNGQGIPVVEHKDEKMFVPSLIFGHLLTSSNYNDEERKVTGGRNGYGAKLCNIFSTKFTVETASKAYKKQFKQTWKDNMTKAEEPKINPFN-GSDFTCITFYPDLAKFKMTVLDKDIVAIMTRRAYDIAGCARGVKVILNDKKLPVKSFKDYISLYLSEKTDDNGDPLKPVHDVVNERWEIAVTLSNSGYQHVSFVNSIATTKGGRHVDYITDQLTSKIAEVIKKKNKGGKDVKPFQIKSHLWVFVNCLIENPTFDSQTKENMTLQIKNFGSKCQLTEKFINQAMKCGVVESIMSWIRFKAQTELEKKCSGKKHSKLKGIPKLEDANDAGTKNSIDCTLILTEGDSAKSLAVSGLGVVGRDKYGVFPLKGKMLNVREASHKQILENAEINNIIKIMGLQYKKKYESIDDLKTLRYGRLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRLPFLEEFITPIVKASKGKEEKCFYSLPEFEEWKTETPNWNKFKVKYYKGLGTSTAKEAKEYFSDMKRHRIRFHYTGNEDDNAIQLAFSKKMVDMRKEWLTNGMEERKRRRELGLPELYLYGKNTTAVTYNDFVNKELILFSNMDNERSIPSAIDGFKPGQRKVLFTCLKRNDKREVKVAQLVGSVAEQSAYHHGEAALAGTIIGLAQDFVGSNNINVLLPVGQFGTRLQGGKDAASPRYIFTMLNSVSKHLFSGLDSPLLHYLYDDNLKIEPEYYVPIIPMVLVNGAEGIGTGWSTKVPNYNPRDLVKNIYKLLDGEDPLPMNPWYKNFRGQIDRLDDQHFLVSGEIAILENNKIEITELPIRTWTQQYKENVLEPMVHGTEKIPAFITDYKEYHTDTTVRFVVTMTPEKFNQAR-EQGFHKVFKLQTSISLSSMVLFDHLGCLRKYDSPEQVLREFYEVRFALYERRKEYMVGVLEAESLKLKNQARFILEKIENKISIENRRRAEMIKTLQDRGYDSDPVKAWKEAQKENIDEEKASESDDEKEIEPSGPDYDYLLGMTLWSLTRERKDDLLKKRDEKIKELDDLKSKSPSDLWRNDLKEFMEELDKVEKEQKEDE--VGSSKLKKKTTGKKKFADEDKPSPFGERINPQIDDDYKKKWEKKPTVPGEVKKGRKKKLPDIKEEDG--------DDIKDV--SLSDRLNTPVKKGKGGAKTAPKQPAGEKKRGAGKSPKKPKRNPWSDDSESNEELSGIS--DMEELGDD 1320 The following BLAST results are available for this feature:
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 1
BLAST of hypothetical protein DAPPUDRAFT_329527 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of hypothetical protein DAPPUDRAFT_329527 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
Pagesback to topAlignments
The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold1323_size47899:33537..38257+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold1323_size47899-snap-gene-0.11 ID=maker-scaffold1323_size47899-snap-gene-0.11|Name=hypothetical protein DAPPUDRAFT_329527|organism=Tigriopus kingsejongensis|type=gene|length=4721bp|location=Sequence derived from alignment at scaffold1323_size47899:33537..38257+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'hypothetical protein DAPPUDRAFT_329527' has the following synonyms
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