This experiment was a double gene knockdown experiment: several candidate genes were targeted via dsRNA injection at the Start Date and a second round of injections followed (22.10.2018).

Efficacy of Knockdown refers to the relative expression of gene EMLSAT00000004598 in 22.10.18. 2 animals were collected (and terminated) at this time to measure gene KD. No pictures were taken of these animals but no phenotype was readily visible. The "Number of Individuals (End)" will reflect the number of animals that survived up at this date (22.10.18): 21.

Surviving animals (21 - 2 terminated animals=19) were then injected with a second dsRNA fragment (F149) targeting EMLSAT00000010881 (22.10.2018). Gene Knockdown for this gene was measured in 30.10.18 (termination date). Surviving animals were 17 and Efficacy of Knockdown for EMLSAT00000010881 was 84%. All images submitted in this form are from animals collected at termination (30.10.18) for which the KD of gene 10881 was measured. Knockdown of gene 4598 was not measured at this point. These pictured animals have therefore underwent two dsRNA injections (fragment F393 followed by fragment F149).