Characterization of the salmon louse Lepeophtheirus salmonis miRNome: Sex-biased differences related to the coding and non-coding RNA interplay

Overview
TitleCharacterization of the salmon louse Lepeophtheirus salmonis miRNome: Sex-biased differences related to the coding and non-coding RNA interplay
AuthorsNúñez-Acuña G, Gallardo-Escárate C
TypeJournal Article
Journal NameMarine genomics
VolumeN/A
IssueN/A
Year2019
Page(s)N/A
CitationNúñez-Acuña G, Gallardo-Escárate C. Characterization of the salmon louse Lepeophtheirus salmonis miRNome: Sex-biased differences related to the coding and non-coding RNA interplay. Marine genomics. 2019 Feb 13.

Abstract

The salmon louse Lepeophtheirus salmonis is a marine ectoparasite that has a detrimental impact on salmon farms. Genomic knowledge of adult stages is critical to understand the reproductive success and lifecycle completion of this species. Here, we report a comprehensive characterization of the L. salmonis miRNome with emphasis on the sex-differences of the parasite. Small-RNA sequencing was conducted on males and females, and mRNA-sequencing was also conducted to identify miRNA-targets at these stages. Based on bioinformatics analyses, 3101 putative miRNAs were found in L. salmonis, including precursors and variants. The most abundant and over-expressed miRNAs belonged to the bantam, mir-100, mir-1, mir-263a and mir-276 families, while the most differentially expressed mRNAs corresponded to genes related to reproduction and other biological processes involved in cell-differentiation. Target analyses revealed that the most up-regulated miRNAs in males can act by inhibiting the expression of genes related to female differentiation such as vitellogenin genes. Target prediction and expression patterns suggested a pivotal role of miRNAs in the reproductive development of L. salmonis.

Author Details
Additional information about authors:
Details
1Gustavo Núñez-Acuña
2Cristian Gallardo-Escárate
Properties
Additional details for this publication include:
Property NameValue
Journal CountryNetherlands
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
CopyrightCopyright © 2019. Published by Elsevier B.V.
DOI10.1016/j.margen.2019.01.005
Elocation10.1016/j.margen.2019.01.005
PIIS1874-7787(18)30184-3
Journal AbbreviationMar Genomics
Publication Date2019 Feb 13
eISSN1876-7478
ISSN1876-7478
Publication ModelPrint-Electronic
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DatabaseAccession
PMID: PMID:30772247