RNAi mediated myosuppressin deficiency affects muscle development and survival in the salmon louse (Lepeophtheirus salmonis)

Overview
TitleRNAi mediated myosuppressin deficiency affects muscle development and survival in the salmon louse (Lepeophtheirus salmonis)
AuthorsKomisarczuk AZ, Kongshaug H, Li M, Nilsen F
TypeJournal Article
Journal NameScientific reports
Volume9
Issue1
Year2019
Page(s)6944
CitationKomisarczuk AZ, Kongshaug H, Li M, Nilsen F. RNAi mediated myosuppressin deficiency affects muscle development and survival in the salmon louse (Lepeophtheirus salmonis). Scientific reports. 2019 May 06; 9(1):6944.

Abstract

Muscle activity is regulated by stimulatory and inhibitory neuropeptides allowing for contraction and relaxation. In Arthropods, one of the important myoinhibitors is Myosuppressin, belonging to FMRFamide-like peptides, that was shown to have inhibitory effects on visceral muscle contraction and to regulate vital physiological processes including reproduction or feeding. We have identified myosuppressin in salmon louse Lepeophtheirus salmonis (LsalMS) and systematically characterised its function and complex abnormalities emerging after LsalMS knockdown by RNAi in all developmental stages in this species. Immunohistochemistry analysis localized the LsalMS mainly to the central nervous system, but also to the vital organs within the alimentary tract and the reproductive system. The most striking feature of LsalMS deficiency during lice development was severe reduction of the muscle content, with abnormalities detected in both the visceral and skeletal muscles. Moreover, down-regulation of LsalMS affects moulting, spermatophore deposition and feeding by affecting development of the intestinal wall and increasing its contraction frequency.

Author Details
Additional information about authors:
Details
1Anna Z Komisarczuk
2Heidi Kongshaug
3Ming Li
4Frank Nilsen
Properties
Additional details for this publication include:
Property NameValue
Journal CountryEngland
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
Elocation10.1038/s41598-019-43515-w
DOI10.1038/s41598-019-43515-w
Journal AbbreviationSci Rep
Publication Date2019 May 06
eISSN2045-2322
ISSN2045-2322
Publication ModelElectronic
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PMID: PMID:31061463