Diapause vs. reproductive programs: transcriptional phenotypes in a keystone copepod

Overview
TitleDiapause vs. reproductive programs: transcriptional phenotypes in a keystone copepod
AuthorsLenz PH, Roncalli V, Cieslak MC, Tarrant AM, Castelfranco AM, Hartline DK
TypeJournal Article
Journal NameCommunications biology
Volume4
Issue1
Year2021
Page(s)426
CitationLenz PH, Roncalli V, Cieslak MC, Tarrant AM, Castelfranco AM, Hartline DK. Diapause vs. reproductive programs: transcriptional phenotypes in a keystone copepod. Communications biology. 2021 Mar 29; 4(1):426.

Abstract

Many arthropods undergo a seasonal dormancy termed "diapause" to optimize timing of reproduction in highly seasonal environments. In the North Atlantic, the copepod Calanus finmarchicus completes one to three generations annually with some individuals maturing into adults, while others interrupt their development to enter diapause. It is unknown which, why and when individuals enter the diapause program. Transcriptomic data from copepods on known programs were analyzed using dimensionality reduction of gene expression and functional analyses to identify program-specific genes and biological processes. These analyses elucidated physiological differences and established protocols that distinguish between programs. Differences in gene expression were associated with maturation of individuals on the reproductive program, while those on the diapause program showed little change over time. Only two of six filters effectively separated copepods by developmental program. The first one included all genes annotated to RNA metabolism and this was confirmed using differential gene expression analysis. The second filter identified 54 differentially expressed genes that were consistently up-regulated in individuals on the diapause program in comparison with those on the reproductive program. Annotated to oogenesis, RNA metabolism and fatty acid biosynthesis, these genes are both indicators for diapause preparation and good candidates for functional studies.

Properties
Additional details for this publication include:
Property NameValue
Journal CountryEngland
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
Elocation10.1038/s42003-021-01946-0
DOI10.1038/s42003-021-01946-0
Journal AbbreviationCommun Biol
Publication Date2021 Mar 29
eISSN2399-3642
ISSN2399-3642
Publication ModelElectronic
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PMID: PMID:33782539