Small, charged proteins in salmon louse (Lepeophtheirus salmonis) secretions modulate Atlantic salmon (Salmo salar) immune responses and coagulation

Overview
TitleSmall, charged proteins in salmon louse (Lepeophtheirus salmonis) secretions modulate Atlantic salmon (Salmo salar) immune responses and coagulation
AuthorsØvergård AC, Midtbø HMD, Hamre LA, Dondrup M, Bjerga GEK, Larsen Ø, Chettri JK, Buchmann K, Nilsen F, Grotmol S
TypeJournal Article
Journal NameScientific reports
Volume12
Issue1
Year2022
Page(s)7995
CitationØvergård AC, Midtbø HMD, Hamre LA, Dondrup M, Bjerga GEK, Larsen Ø, Chettri JK, Buchmann K, Nilsen F, Grotmol S. Small, charged proteins in salmon louse (Lepeophtheirus salmonis) secretions modulate Atlantic salmon (Salmo salar) immune responses and coagulation. Scientific reports. 2022 May 14; 12(1):7995.

Abstract

Little is known about glandular proteins secreted from the skin- and blood-feeding ectoparasite salmon louse (Lepeophtheirus salmonis). The labial gland has ducts extending into the oral cavity of the lice, and the present study aimed to identify novel genes expressed by this gland type and to investigate their role in modulation of host parameters at the lice feeding site. Five genes associated with labial gland function were identified and named Lepeophteirus salmonis labial gland protein (LsLGP) 1-4 and 1 like (LsLGP1L). All LsLGPs were predicted to be small charged secreted proteins not encoding any known protein domains. Functional studies revealed that LsLGP1 and/or LsLGP1L regulated the expression of other labial gland genes. Immune dampening functions were indicated for LsLGP2 and 3. Whereas LsLGP2 was expressed throughout the parasitic life cycle and found to dampen inflammatory cytokines, LsLGP3 displayed an increased expression in mobile stages and appeared to dampen adaptive immune responses. Expression of LsLGP4 coincided with moulting to the mobile pre-adult I stage where hematophagous feeding is initiated, and synthetic LsLGP4 decreased the clotting time of Atlantic salmon plasma. Results from the present study confirm that the salmon louse secretes immune modulating and anti-coagulative proteins with a potential application in new immune based anti-salmon louse treatments.

Properties
Additional details for this publication include:
Property NameValue
Journal CountryEngland
Publication TypeJournal Article
Language Abbreng
LanguageEnglish
Copyright© 2022. The Author(s).
Elocation10.1038/s41598-022-11773-w
DOI10.1038/s41598-022-11773-w
Journal AbbreviationSci Rep
Publication Date2022 May 14
eISSN2045-2322
ISSN2045-2322
Publication ModelElectronic
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PMID:35568726