BLAST of predicted protein vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 203.371 bits (516), Expect = 4.593e-58
Identity = 137/500 (27.40%), Postives = 256/500 (51.20%), Query Frame = 0

Query:  123 KSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAINFLVPKT--NVVTTTCFFHVGGF----FTGIN--ACLKYQTYYHMFGP----SFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEY---DGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESG-IISAGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAM-MKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQR-HPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWA 604
            +S++ ++ +    +   +  +NG +KGDV+H+++GN    +       +  G +S+   N+   ++  ++   Q +FVIC   T   V          +D+ +  +  + +   + ++  +   T+ P+ ++ D++  + VI W+SGTTG PK I  +  S  N     + FL PKT  N++ T  F HV GF    F  IN   C      +H   P    S  ++ ++  +S++   T+ + + H V ++E    D    ++  +FD ++ + PAGA          ++  RNL+ V N Y  TESG +I      ++ G ++ N+ V + ++ +GER  P+  GEL Y+ P     GYLNRP +   F   +G+  SGD+ +Y   G++++  R+KE++K   +H+ P E+E I+Q  +  + E+ +F K  +++Q ++  +VV+K  ++    E+ +FVN  + DYK +   I+  + +PRN  GKL+R ++   A
Sbjct:   72 ESVSILDFIEKVPKFAKYLYENGLRKGDVVHIIIGNRGDVYIPVHATLLCQGVVSISVDNISTETLLSEVQETQAKFVICTNRTYYKV----------KDLSSIIINLDTQLHAILEIDMSTQ-TQIPEQIEFDLQT-LAVILWTSGTTGKPKGIQLNMVSLWN-----LLFLSPKTDGNMLITCNFAHVSGFNYSCFWSINFDVCT-----FHFISPEDVTSLGVEKIIFTLSQIPFTTIVVSASHIVYIAEKIVNDQIEYSS--NFDEVKNICPAGAHTYPGISDDLRKLFRNLENVPNLYAMTESGGLILISKDQRSNGGVVGNMEVYIRDIHSGERLGPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSK-TEKIQTILGGIVVIKKGYRIDPEEIRQFVNDRIDDYKHLEIIILTNEPLPRNPNGKLLRNDINKLA 546          

BLAST of predicted protein vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 186.037 bits (471), Expect = 1.745e-51
Identity = 138/508 (27.17%), Postives = 240/508 (47.24%), Query Frame = 0

Query:  120 LTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWI-LGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRN--------IDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAIN----FGGPAI-NFL---VPKTNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANP----DDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIISA-----GVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMR 601
            +TG++ T     +  ++ G+  L+ G KKGDV+ +++ N    F I F G   +G  ++  +    P  +A QL+    ++V+ +    + + ++ ++ S  Q  K   +G  E   D    +           D  E  DP      +D+  + +SSGTTG PK +  +H++ +N       P+I NF+   V +   +    FFH+      ++A L++        P F  +  + ++ + KP        H + +     F  ++P        SI+ L    +    +    +  KC +       YG TE   ++      G+   + G+++PN   K+ ++ TG+   P E GELC   P +M+GY    K   D +   G+ H+GDI YY  DG  Y VDRLKELIK K   +AP+ELED+++++P V +    G  ++   EL  A VV K      E  +I+FV  +V  +K++   ++F D +P+N  GK++RRE++
Sbjct:   83 MTGRTYTYEMVHSLSQKFGSALLRLGAKKGDVMGLVVPNIP-EFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEIYSGIQ--KIIVIGMEETPSDCKSFMEMAIFDDGSFFDQRETIDP-----HKDILALPYSSGTTGPPKGVALTHSNLVNNITQLSHPSIHNFIGDEVEQHISIAVLPFFHIFAMTCSLSAGLRFGVKLITL-PKFEPEMFINSLVQYKPT-------HMLVVPPLVSFMTSSPLIKPSLLSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQEGYGMTECSPVTHVQPKDGIIYGSCGNLIPNTIGKIIDIDTGKVLGPGENGELCVSGPQIMQGYYKN-KKATDKIIQNGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVVRK-NRSVMEQGIIDFVTEKVAPHKKLGAGVMFVDTLPKNQTGKILRRELK 572          

BLAST of predicted protein vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 142.51 bits (358), Expect = 1.008e-36
Identity = 118/481 (24.53%), Postives = 211/481 (43.87%), Query Frame = 0

Query:  137 LGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPDP-MDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAIN--FGGPAINFLVPKTNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESG---IISAGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCY-KTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQR-HPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDE-IPRNSVGKLMRREMRDWARHQM 608
            L     + G +K DV+H+++GN    +   F   +  G +S+   ++   ++  +LN  Q   V C   T K V                C   N +  D+ +++ N   ++   P  D+   +DV VI WSSGTTG PK +  +  S ++  F  P +     K N++ +  F H+ GF + +     + T    +  S  + H                                    F  ++        VP                  N YG TESG   ++S    S   G ++      + +++TG+   P++ GEL + K  A   GYLNR ++N    + +G+ H+GD  +YT  G+ +F  R+ E+IK + +H+ P ELE +L+  H  + E  V     ++ Q ++ A++V+K   +     + + +N++V +YKRI G ++   E +PRN++GKL+R E++   +  +
Sbjct:   86 LAKSLYEKGLRKSDVVHLIIGNRADIYLPVFATILCQGVISISVDSISHDTLMTELNDTQAEIVFCTNKTFKKVKNC------------GCTIINMD-TDIEEMIANGCTSKVIVPKQDVFSLKDVFVILWSSGTTGQPKGVQMTVVSFLDSMFATPKV-----KGNMLLSANFGHISGFISSLFWTFHFSTSTFYYISSDDVKH------------------------------------FGVVKLFEVLKKCVP------------------NFYGSTESGGGFLVSTNQHSN--GGVVARTETYIRDLNTGQHLGPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVV-PVVEKKQTVLGAIIVVKNDKKINVETIRKELNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEIQKLFKSSL 491          

BLAST of predicted protein vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 139.813 bits (351), Expect = 3.690e-35
Identity = 124/501 (24.75%), Postives = 224/501 (44.71%), Query Frame = 0

Query:  136 RLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFCLGKNEEFED----LNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAIN-FGGP--AINFLVPKTNVVTTTC---FFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIISAGVSSQALGSI-----------------LPNIRVKVENVSTGERC---APHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWARH 606
            ++ +   +NG  KGDV+ +   N    + +  G W  GG +   +  L P  +  Q+   +T+ ++C  D    V     L +     K   +G     +D    + DLL N D  + P  +  D  +DV  + ++S ++G  K I H++ S ++ F  P  A N    +    +  C   FFH+ GF+      +   + Y +    F   + L A+   K  +++  +     LS+     +    D  S++ L      +  +      ++  ++K V  AYG  E G+++     +   SI                 LPN+   ++ +S  +      P ++GE+C K+P  M GYLN+ + +A   D + + H+GD+ YY   G +YFV+++  LI++    +AP+ LE  L     + ++ V G  N E  ++  A VVLKP  + TE  L   +   + D++RIRG + F   IPR+   K++R  + ++  H
Sbjct:   99 KVASGLTKNGFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVC--DDFNYVDGLDFLENTNMIEKLIIIGNECTAQDKVILMTDLL-NDDGKKVPQKVIFDWAKDVIYMPFTSTSSG-AKGILHTNKSLMSQFYSPDGAANHWFDQLIGDSIHCANYFFHMTGFYNFALGSIYGISIYTL--SEFSEQNFLEALVDXKTASISCYAWQIRILSQSPLVSNY---DLSSLKVLMTGCGLLSSTIRMEVLDRIPSIKYVREAYGLNECGLVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYLNKEESSA--FDNDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLRSVLAEYKPH 588          

BLAST of predicted protein vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 122.865 bits (307), Expect = 9.770e-30
Identity = 132/520 (25.38%), Postives = 215/520 (41.35%), Query Frame = 0

Query:  111 GDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPDPMD-----MDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPA--------------INFLVPKTNVVTTTCFFHVGGFFTGINACL-KYQTYYHMFGPS----FRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFD--SIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIISAGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPA-VQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELI---EFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREM 600
            G +  + N     S++  E + Y KR   F  +          +LL N    F    G W LG  +SL     +P S  E   T + + +I        + E L L       +TF + K         + R I  T  P  M      M+ + +V  IF   GTT     I     SA N                    N+      ++  T FFH GGF  G+   L + +T   +  P+    F   HL  A+   KP  +       V++   +G  +     FD  S+  + P G ++  + E   + +  +LK +   YG TE+  IS G+S Q LG + P  ++++ N   GE   P+  G++  K    M+ Y+    D+   +  +GF + GD+ YY   G I+F  R K+L+K  N+H+ P E+E  +    + V++  VFG+ +    E +S V+VL    +    + +    F   +   YK ++G +     IPRN+ GK+ R  +
Sbjct:   56 GSFLSYENEHRSNSISPREFVKYTKRFAHFISERHGNARFATVLLLNNTRFFFIPVVGTWFLGSSVSL----FQPSSSRE---TIERQLMI--------LKEELNLEEIVVITETFAIPKISNI----GVDRIISLTPVPGYMSLQEIFMNDDNEVVEIFNREGTTSLQSEIAGVLYSAENILNSIHYCSYVPFQIHHVIKNYEPSSLRLMILTNFFHPGGFTEGLRNILPQKRTGIILRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKM---NGASNDLSSQFDLSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTETLSISLGISDQHLGRLFPKYQLRILN-PNGEPLGPNSVGDIVVKPGYGMRRYIG---DHEYNITQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDKNGIERLSGVIVLHHIEKGLSLQNVLERTFEAYKFPRYKWLQGGVRVTSAIPRNANGKIFRGRL 549          

BLAST of predicted protein vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 85.1149 bits (209), Expect = 2.456e-17
Identity = 65/237 (27.43%), Postives = 111/237 (46.84%), Query Frame = 0

Query:  374 DFDSIEFLAPAGA-AVPQSCEAIYKEKCRNLKGVLNAYGQTESGII--------SAGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMR 601
            D  S+E     G+  + + CE    EK  N+K ++N YG TE+G          S  V    +G  L ++  K+ +   G+    +  GEL  +TP +M GY    K +   +  + + HSGD+   T  G    + RL +++     +I P E+ED L +HP + E  + G  +D + E + A + L  +  A E +L E    ++  +K  +  +  +D  P+   GK+ + EMR
Sbjct:  679 DVSSVELGITGGSNCLVEVCEDF--EKHLNMKSLMNIYGATETGPATFVPSPEDSFEVRINTVGPPLGHVEAKIVD-QNGKILPVNTPGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEELCAWICL--EEGAKEFDLKEHCKGKIAHFKIPKYSMFVKD-FPKTLSGKVKKLEMR 909          

HSP 2 Score: 83.5741 bits (205), Expect = 8.153e-17
Identity = 58/212 (27.36%), Postives = 105/212 (49.53%), Query Frame = 0

Query:  398 EKCRNLKGVLNAYGQTESGIISA--------GVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMR 601
            EK  N+K ++N YG TE+G +S          V    +G  L +I  K+ +   G+    +  GEL  +TP +M GY    K +   +  + + HSGD+   T  G    + RL +++     +I P E+ED+L +HP++ E  + G  ++ + E + A + L  +  A E +L E    +++ +K  +  +  +D  P+   GK+ + EM+
Sbjct:  331 EKHLNMKSLMNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVD-QNGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEELCAWICL--EEGAKEFDLKEHCKGKISHFKIPKYPMFVKD-FPKTLSGKVKKFEMK 538          

HSP 3 Score: 82.0333 bits (201), Expect = 2.142e-16
Identity = 86/349 (24.64%), Postives = 140/349 (40.11%), Query Frame = 0

Query:  264 IFWSSGTTGHPKAICHSHNSAIN---FGGPAINFLVPKTNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIISA--------GVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMR 601
            I ++SGTTG PKA+  SH   IN     G  I F +    +      FH  G  TG    L Y +   +    F     L  +   K   L      Y+ + E                                 ++K RN+      YG TE+G +S          V    +G  L +I  K+ +   G+    +  GEL  +TP +M GY    K +   +  + + HSGD+   T  G    + RL +++     +I P E+ED L +HP++ E  + G  +D + E + A + L  +  A E +L E    ++  +K  +  +  +D  P+   GK+ + EM+
Sbjct:    4 IQFTSGTTGLPKAVALSHFCVINNALLTGNRIGFDLKYHXINLWVPLFHTFGCVTGTLISLCYGSKLVLPSDGFDPTACLNCIPAEKCTYLFGTPTMYIAVLEA--------------------------------QKKLRNI------YGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVD-QNGKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEELCAWICL--EEGAKEFDLKEHCKGKIAHFKIPKYPMFVKD-FPKTLSGKVKKFEMK 310          

BLAST of predicted protein vs. L. salmonis genes
Match: EMLSAG00000004507 (supercontig:LSalAtl2s:LSalAtl2s235:163315:165883:-1 gene:EMLSAG00000004507 transcript:EMLSAT00000004507 description:"maker-LSalAtl2s235-augustus-gene-1.27")

HSP 1 Score: 72.7886 bits (177), Expect = 1.091e-13
Identity = 102/437 (23.34%), Postives = 181/437 (41.42%), Query Frame = 0

Query:  207 QKLVTEALKLASRPQDIKTFCLGKN--------EEFED--LNDLLRNIDPTEC-PDPMDMDVEEDVPVIFWSSGTTGHPKAICHS-----------HNSAINFGGPAINFLVPKTNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKC-RNLKGVLNAYGQTESGIISAGVSSQALGS-ILPNIRVK--------VENVSTGERCAPHEEGELCYKTP--AMMKGYLNRPKDNAD--FLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQAT---EAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWA 604
            +++V EA+K +S+P  IK   + K         +   D  L D +  ++ +EC P+ M     ED     ++SG+TG PK + HS           H    +F    I   V     +T   +   G    G  + L +++      PS       + + +L             QL +Y+  +  N D   S+  L   G  +       Y +   RN   +++ + QTE+G I        L + I+P + ++        + +    E    +++G LC KTP   M +   +  K   +  F  F G+  +GD  Y T +G I  V R+ ++I    + +   E+ED+L +H  + E+ V G  +D   E + A VV K   +     E  + + V   ++ +  +     F +++P+   GK+MRR +R  A
Sbjct:  144 KEVVDEAIKKSSQP--IKHVLVAKRSGKGHVKMKSGRDVYLEDAMLQVE-SECHPESMS---SEDPLFYLYTSGSTGAPKGLVHSTAGYLLYTSFTHKIIFDFCEHDIFGCVADIGWITGHSYIVYGPLMNGGTSVL-FESTPTYPDPS----RYWKLVEELGITQFYTSPTAIRQLMKYEDSYVLNSDK-SSLRTLGSVGEPINIEAWNWYNDLVGRNRCDIVDTWWQTETGGICITPRPSELNAEIVPALGMRPFLGINPVLLDDKGNEIKENNKQGSLCLKTPWPGMARSIYDDHKRYQETYFSAFPGYYFTGDGAYRTKEGYISIVGRIDDVINVSGHRLGTAEVEDVLTKHDNIVEAAVVGXPHDIKGEAIYAFVVPKNLDEGVKKFEKGIQKLVKDNISGFA-VPEKFQFCEDLPKTRSGKIMRRILRKIA 567          

BLAST of predicted protein vs. L. salmonis genes
Match: EMLSAG00000003336 (supercontig:LSalAtl2s:LSalAtl2s182:521806:564306:1 gene:EMLSAG00000003336 transcript:EMLSAT00000003336 description:"maker-LSalAtl2s182-augustus-gene-5.28")

HSP 1 Score: 55.0694 bits (131), Expect = 5.088e-8
Identity = 89/401 (22.19%), Postives = 153/401 (38.15%), Query Frame = 0

Query:  228 LGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAI----NFLVPKTNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLD-HLLRAMSKLKPKTLAIG-----------------SHHYVQLSEYDGFHDANPDDF------------DSIEFLAPAGAAVPQSC------EAIYKEKCRNLKGVLN-----AYGQTESGIISAGVSSQ----ALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKE-LIKYKNNHIAPTELEDILQRH-PAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRI 577
            LG+ E  + LND L +I   +C              + ++SGTTG+PK +  SH+  I F   AI    ++   K  VV+     H+   F  I   L  +   + F   + L   L+  + +++P T+  G                 +    Q+     F DA+   +            D + F     A     C       A   E+ R+    L+      YG +E GI  +   S       G+I+P+   KV   S  +       GE+C K   +M GYL   +  ++  D EG+ H+G               RLK+ L+     ++AP  +E+ ++ + P V  ++V G    + ++ +S  + +K K         E  +    D+K +
Sbjct:  197 LGRKEGIDRLNDRLSHISVNQCCS------------LIYTSGTTGNPKGVMLSHD-IITFMTKAIVKKHDWTTAKFIVVSYLPLSHIAASFLDIYVALYLKGEVY-FADKYALKGSLVDTLKEVRP-TMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQKSDFKTGTCGTIIPSTHAKVVKSSDDDI-----GGEICIKGRCVMMGYLFNEQATSEAFDKEGYFHTG---------------RLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVG----DAEKFISVXLTIKVKSNEDGEPTSELTDSVKKDFKNL 558          

BLAST of predicted protein vs. SwissProt
Match: gi|74897042|sp|Q54P78.1|4CL2_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 2; Short=4CL 2; AltName: Full=4-coumaroyl-CoA synthase 2)

HSP 1 Score: 181.8 bits (460), Expect = 1.034e-48
Identity = 141/485 (29.07%), Postives = 227/485 (46.80%), Query Frame = 0

Query:  147 KKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVIC---------------MPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAINFLVPKTN------VVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSE---YDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESG----IISAG-VSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRD 602
            KKGDV+ V+L N     PI  G  ++GG  SL + +     ++  L T   R++                  P  +K++   +      ++I    L  +      N L+ N +  + P  + +D+++D  +I +SSGTTG  K +C SH++ ++         V  TN      V+    FFH+ G  T +   +K Q +  +  P F     L  + K K     I     +  ++    D F      D  S+  L    A + +  E + KE+ +    +   YG TE      +I +G + S + G +LPN  VK+ +  TGE     E+GE+C K P +M GY N  K   + +D +GF  +GDI Y   DG  + VDR KELIK K   + P ELE +L  HP V ++ V G    ++ E+    VV+K     TE EL+++ + ++ +YK  RG I F   IP+++ GKL+R+ ++D
Sbjct:   73 KKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIA-GQTLKEIGQLTLSSDGIVMSFNQLINN-NGKDYPI-VRIDLKKDTAIIPFSSGTTGLFKGVCLSHHNLVS--NTHQTQTVETTNYKKNDTVMGQLPFFHIYGLMTYLILMVK-QGHCVVILPKFEFVRFLDLIQKYKVAISFIVPPIAIMFAKSPIVDKF------DLSSLRTLFSGAAPLSREVEDLIKERFKGKLIIKQGYGATELSPACFVIPSGLIKSGSAGILLPNQLVKIISPETGENLGMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFFKTGDIGYVDEDGYYFIVDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKD 545          

BLAST of predicted protein vs. SwissProt
Match: gi|182627620|sp|Q54P79.2|4CL3_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 181.415 bits (459), Expect = 1.540e-48
Identity = 140/488 (28.69%), Postives = 224/488 (45.90%), Query Frame = 0

Query:  147 KKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVIC---------------MPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAIN--FGGPAINFLVPKTN--VVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSE---YDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTE---------SGIISAGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDW 603
            KKGDV+ V+L N     PI  G  ++GG  SL + +     ++  L T   R++                  P  +K++   +      ++I    L  +      N L  N +  + P  + +D+ +D  +I +SSGTTG  K +C SH + ++  +    I     K N  V+    FFH  G    I   +K Q Y  +  P F     L  + K K     I     +  ++    D F      D  S+  L    A +    E + KE+ +    +   YG TE         +G++ +G S    G++LPN+  K+ +  TGE     E+GE+C K P +M GY N  K   + +D +GF  +GDI Y   DG  + +DR KELIK K   + P ELE +L  HP V ++ V G    ++ E+    VV+K     TE EL+++ + ++ +YK  RG I F   IP+++ GKL+R+ ++D+
Sbjct:   73 KKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIA-GQTLKEIDQLTLSSDGIVMSFNQLTNN-NGKDYPI-VRIDLTKDTAIIPFSSGTTGLFKGVCLSHYNLVSNTYQTQTIETSTYKKNDSVIGVLPFFHSFGLMLHIMLMVK-QGYRIVTLPKFEPVRFLELIKKYKVAMSFIVPPIAIMFAKSPIVDKF------DLSSLRTLFCGAAPLGSEIEDLIKERFKGRLVIKQGYGATELSPCCFVTPNGLVKSGSS----GTLLPNLLAKIISSETGENLGMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFLKTGDIGYVDEDGYFFIIDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKDF 546          

BLAST of predicted protein vs. SwissProt
Match: gi|126501|sp|P08659.1|LUCI_PHOPY (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 181.03 bits (458), Expect = 1.758e-48
Identity = 148/536 (27.61%), Postives = 245/536 (45.71%), Query Frame = 0

Query:   93 GSLYQMVYRAMERYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFV-ICMPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPD-------PMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSA-INFG---GPAI-NFLVPKTNVVTTTCFFHVGGFFT-------GINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSC-EAIYKEKCRNLKGVLNAYGQTE--SGII---SAGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRD 602
            G+  + +++AM+RY    G    F +     ++T  E      RL     + G      I V   N    F    G   +G  ++  +     R +   +N  Q   V +     QK++    KL   P   K   +    +++    +   +     P        P   D ++ + +I  SSG+TG PK +   H +A + F     P   N ++P T +++   F H  G FT       G    L Y+    +F  S +   +  A+  L P   +     + + +  D +      D  ++  +A  GA + +   EA+ K    +L G+   YG TE  S I+          A+G ++P    KV ++ TG+    ++ GELC + P +M GY+N P+     +D +G+ HSGDI Y+  D   + VDRLK LIKYK   +AP ELE IL +HP + ++ V G  +D+  EL +AVVVL+     TE E++++V  +VT  K++RG ++F DE+P+   GKL  R++R+
Sbjct:   20 GTAGEQLHKAMKRYALVPGTI-AFTDAHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKL---PIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSAL--LVPTLFSF----FAKSTLIDKY------DLSNLHEIASGGAPLSKEVGEAVAKR--FHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIRE 537          

BLAST of predicted protein vs. SwissProt
Match: gi|74897041|sp|Q54P77.1|4CL1_DICDI (RecName: Full=Probable 4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 180.644 bits (457), Expect = 2.460e-48
Identity = 140/485 (28.87%), Postives = 225/485 (46.39%), Query Frame = 0

Query:  146 CKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVIC---------------MPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAIN--FGGPAINFLVPKTN--VVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSE---YDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESG----IISAG-VSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDW 603
             KKGDV+ V+L N     PI  G  ++GG  SL + +     ++  L T   R++                  P  +K++   +      ++I    L  +      N L+ N +  + P  + +D ++D  +I +SSGTTG  K +C SH++ ++  +    I     K N  V+    FFH+ G    +   +K Q +  +  P F     L  + K K     I     +  ++    D F      D  S+  L    A +    E + KE+ +    +   YG TE      +I +G V S + G +LPN   K+ +  TGE     E+GE+C K P +M GY N  K   + +D +GF  +GDI Y   DG  + VDR KELIK K   + P ELE +L  HP V ++ V G    ++ E+    VV+K     TE EL+++ + ++ +YK  RG I F   IP+++ GKL+R+ ++D+
Sbjct:   72 IKKGDVLGVILPNLPEYVPIFHGTLLMGGITSLVNPDYTIEELSHTLATVSPRYLAVTLAVYEKIKNDLKRVFPSVEKVILVDIA-GQTLKEIDQLTLSSDGIVMSFNQLINN-NGKDYPI-VRIDPKKDTAIIPFSSGTTGLFKGVCLSHHNIVSNTYQTQTIETSTYKKNDTVMGILPFFHIYGLMLFLMLMVK-QGHRVVVLPKFEPVRFLELIQKYKVAISFIVPPVAIMFAKSPIVDKF------DLSSLRTLFSGAAPLSSEVEDLIKERFKGRLVIKQGYGATELSPACFVIPSGLVKSGSAGILLPNQLAKIISPETGENLGMGEKGEICIKGPNVMLGYYNNEKATNEVIDKDGFLKTGDIGYVDEDGYYFIVDRSKELIKCKGFQVPPAELEALLLSHPKVADACVVGLSKGDMGEVPRGFVVIKQNESLTEKELLDWAHPKIANYKHFRGGIFFIPAIPKSATGKLLRKNLKDF 546          

BLAST of predicted protein vs. SwissProt
Match: gi|75311763|sp|Q9M0X9.1|4CLL7_ARATH (RecName: Full=4-coumarate--CoA ligase-like 7; AltName: Full=4-coumarate--CoA ligase isoform 6; Short=At4CL6)

HSP 1 Score: 178.718 bits (452), Expect = 1.299e-47
Identity = 136/520 (26.15%), Postives = 244/520 (46.92%), Query Frame = 0

Query:  121 TGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICF-GGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICM----PDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAINFLVPKTNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPS------------------FRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVL--NAYGQTES-GIIS------AGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWARHQM 608
            TG S+T  +  +   RL   F + G +K DV+ +   N +  FP+CF     +GG  +  +       V++Q+     + +I +       +      + L S+  D      G N +    ++++   +P     P     + D   + +SSGTTG  K +  +H           NF+     V          G + G+  C  +   +H+FG +                  F L+ +L+ + K +   L +    ++ LS+          D  S++++    A + +    + +E  RN+  VL    YG TE+ GI+S         +S + G + P +  ++ +V TG+   P+++GE+  + P MMKGYLN P+   + +D + + H+GD+ Y+  DG++Y VDR+KELIKYK   +AP ELE +L  HP + +++V    ++E  E+  A VV  P    TE ++ +F+ ++V  YKR+R  + F   +P+++ GK++RRE+    R +M
Sbjct:   51 TGDSLTFSQLKSAVARLAHGFHRLGIRKNDVVLIFAPN-SYQFPLCFLAVTAIGGVFTTANPLYTVNEVSKQIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSK--DTVEIPPGSNSKILSFDNVMELSEPV-SEYPFVEIKQSDTAALLYSSGTTGTSKGVELTHG----------NFIAASLMVTMDQDLM---GEYHGVFLC--FLPMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSIVKKF---DLSSLKYIGSGAAPLGKD---LMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRNSGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWVHTGDLGYFNEDGNLYVVDRIKELIKYKGFQVAPAELEGLLVSHPDILDAVVIPFPDEEAGEVPIAFVVRSPNSSITEQDIQKFIAKQVAPYKRLR-RVSFISLVPKSAAGKILRRELVQQVRSKM 544          

BLAST of predicted protein vs. SwissProt
Match: gi|126500|sp|P13129.1|LUCI_LUCCR (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 172.17 bits (435), Expect = 2.471e-45
Identity = 115/364 (31.59%), Postives = 189/364 (51.92%), Query Frame = 0

Query:  253 MDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAI-----NFLVPKTNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSC-EAIYKEKCRNLKGVLNAYGQTE--SGII---SAGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWAR 605
            +++D +E V +I  SSG+TG PK +  +H + +     A      N + P T V+T   F H  G FT +   +    +  +    F  +  L+ +   K  ++ +    +  L++ +     N  D  ++  +A  GA + +   EA+ +    NL GV   YG TE  S II          A G ++P  + KV ++ T +   P+  GE+C K P +MKGY+N P+   + +D EG+ H+GDI YY  +   + VDRLK LIKYK   + P ELE +L +HP++ ++ V G  +    EL  AVVVL+     TE E++++V  +V++ KR+RG + F DE+P+   GK+  R +R+  +
Sbjct:  186 VEVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFSHARDPIYGNQVSPGTAVLTVVPFHHGFGMFTTLGYLI--CGFRVVMLTKFDEETFLKTLQDYKCTSVILVPTLFAILNKSELL---NKYDLSNLVEIASGGAPLSKEVGEAVARR--FNLPGVRQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGPNRRGEVCVKGPMLMKGYVNNPEATKELIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPSIFDAGVAGVPDPVAGELPGAVVVLESGKNMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKIDGRAIREILK 542          

BLAST of predicted protein vs. SwissProt
Match: gi|75296548|sp|Q7XXL2.2|4CLL9_ORYSJ (RecName: Full=4-coumarate--CoA ligase-like 9)

HSP 1 Score: 169.859 bits (429), Expect = 1.936e-44
Identity = 152/523 (29.06%), Postives = 249/523 (47.61%), Query Frame = 0

Query:  122 GKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVA----EQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFCLGKNEEFE---DLNDLLRNIDPTECPD-PMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAINFLVPKT--NVVTTTC--FFHVGGFFTGI-NACLKYQTYYHMFGPSFRLDHLLRAMSK--------LKPKTLAIGSHHYVQLSEYDGFHDANPDDFD----SIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGII----SAG------VSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWARHQMR 609
            G+S T  E      R        G +KG V+ V L N  + +P+   G +  G +  G   + PR++A    +Q+   + + V+      ++  + +K A  P       +G  E        + LL   D T     P+D   + D+  + +SSGTTG  K +  SH + ++    ++  + P+T   VVT     FFH+ G  TGI  A L+++    +    F L   LRA+          + P  LA+                   D+FD    +++ +  A A +     A ++ K   ++ V  AYG TE   I    +AG          ++G ILPN+ VK  +  TG     +  GELC ++ ++M+GY  R ++    +D +G+ H+GD+ Y   DGD++ VDR+KELIKYK   +AP ELE +L  HP+V+++ VFG  ++E  E+  A VV +   +  E E++ +V   V  YKR+R   I  D IP++  GK++RR++RD    +M+
Sbjct:   59 GRSYTYGEVARDTARFARALRSVGVRKGHVVVVALPNLAV-YPVVSLGIMSAGAVFSG---VNPRALAAEIKKQVEDSEAKLVVA----NEVAFDKVKDAGVP----VIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSDLCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYG-ITGICCATLRHKGTVVVMD-RFDLRTFLRALVDHRVMFAPLVPPVMLAM-------------VKSPVADEFDLSDLALKSVMTAAAPLAPDLLAAFQRKFPGVQ-VEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGFILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVGYIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVACVVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIV-DAIPKSVSGKILRRQLRDEFIKRMK 552          

BLAST of predicted protein vs. SwissProt
Match: gi|61213879|sp|Q26304.1|LUCI_LUCMI (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 166.392 bits (420), Expect = 2.917e-43
Identity = 118/370 (31.89%), Postives = 194/370 (52.43%), Query Frame = 0

Query:  252 PMDM-DVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAI-----NFLVPKTNVVTTTCFFHVGGFFT--GINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFD--SIEFLAPAGAAVPQSC-EAIYKEKCRNLKGVLNAYGQTESG---IISAGVSSQ--ALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWAR 605
            P+D+ + ++ V ++  SSG+TG PK +  +H  A+     A      N + P T ++T   F H  G FT  G  AC     Y  +    F  +  LR +   K  ++ +    +  L++ +       D FD  ++  +A  GA + +   EA+ +    NL GV   YG TE+    II+     +  A G ++P  +VKV ++ T +    +  GE+C K P++M GY N P+   + +D EG+ H+GDI YY  D   + VDRLK LIKYK   + P ELE +L +HP + ++ V G  + +  EL  AVVV++     TE E++++VN +V ++KR+RG + F DE+P+   GK+  + +R+  +
Sbjct:  184 PIDVKNRKQHVALLMNSSGSTGLPKGVRITHEGAVTRFSHAKDPIYGNQVSPGTAILTVVPFHHGFGMFTTLGYFAC----GYRVVMLTKFDEELFLRTLQDYKCTSVILVPTLFAILNKSELI-----DKFDLSNLTEIASGGAPLAKEVGEAVARR--FNLPGVRQGYGLTETTSAFIITPEGDDKPGASGKVVPLFKVKVIDLDTKKTLGVNRRGEICVKGPSLMLGYSNNPEATRETIDEEGWLHTGDIGYYDEDEHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPDAGELPGAVVVMEKGKTMTEKEIVDYVNSQVVNHKRLRGGVRFVDEVPKGLTGKIDAKVIREILK 542          

BLAST of predicted protein vs. SwissProt
Match: gi|266484|sp|Q01158.1|LUCI_LUCLA (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 166.392 bits (420), Expect = 2.975e-43
Identity = 145/536 (27.05%), Postives = 243/536 (45.34%), Query Frame = 0

Query:   92 QGSLYQMVYRAMERYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFCLGKNE----EFEDLNDLLRNIDPT----ECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAI-----NFLVPKTNVVTTTCFFHVGGFFTGINACLKYQT--YYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCR--NLKGVLNAYGQTE--SGII---SAGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWAR 605
            +GS    + + M+RY +       F N LTG   T  E L     LG      G      I +   N    F     G  +G  ++  +     R +   L   +   V     ++K + + + +      IKT  +  ++     ++ +++ ++   P          ++++ +E V +I  SSG+TG PK +  +H +A+     A      N + P T ++T   F H  G FT     L Y T  +  +    F  +  L+ +   K  ++ +    +  L+  +       D +D    +  A    P S E I +   R  NL GV   YG TE  S II          A G ++P  + KV ++ T +   P+  GE+C K P +MKGY++ P+   + +D EG+ H+GDI YY  +   + VDRLK LIKYK   + P ELE +L +HP + ++ V G  +    EL  AVVVL+     TE E++++V  +V++ KR+RG + F DE+P+   GK+  + +R+  +
Sbjct:   22 EGSAGAQLRKYMDRYAKLGA--IAFTNALTGVDYTYAEYLEKSCCLGEALKNYGLVVDGRIALCSENCEEFFIPVLAGLFIGVGVAPTNEIYTLRELVHSLGISKPTIVFS---SKKGLDKVITVQKTVTAIKTIVILDSKVDYRGYQSMDNFIKKNTPQGFKGSSFKTVEVNRKEQVALIMNSSGSTGLPKGVQLTHENAVTRFSHARDPIYGNQVSPGTAILTVVPFHHGFGMFT----TLGYLTCGFRIVMLTKFDEETFLKTLQDYKCSSVILVPTLFAILNRSELL-----DKYDLSNLVEIASGGAPLSKE-IGEAVARRFNLPGVRQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKTLGPNRRGEVCVKGPMLMKGYVDNPEATREIIDEEGWLHTGDIGYYDEEKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPNIFDAGVAGVPDPIAGELPGAVVVLEKGKSMTEKEVMDYVASQVSNAKRLRGGVRFVDEVPKGLTGKIDGKAIREILK 542          

BLAST of predicted protein vs. SwissProt
Match: gi|41688574|sp|Q27757.2|LUCI_PHOPE (RecName: Full=Luciferin 4-monooxygenase; Short=Luciferase)

HSP 1 Score: 166.007 bits (419), Expect = 4.008e-43
Identity = 157/546 (28.75%), Postives = 252/546 (46.15%), Query Frame = 0

Query:   93 GSLYQMVYRAMERYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMS-LGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFC-LGKNEE---FEDLNDLLR-----NIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAI---------NFGGPAINFLVPKTNVVTTTCFFHVGGFFT--GINACLKYQTYYHMFGPSFRLDHL--LRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTE--SGII---SAGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRD-WARHQMR 609
            G+  + ++ A+ RY +  G      N  T +++   E L    RL   F + G K+ D I V   N    F        LG   + + D  +E R +   L   + R + C  +T + V   L + S+ + ++T   L  NE+   ++ LN+ +      N+D  +   P   + ++ V ++ +SSGTTG  K +  +H + +          FG  AIN   P T ++T   F H  G  T  G   C       H F     L  L   +  S L   TL         + +YD  H         ++ +A  GA + +    + K++ + L  V   YG TE  S ++      V   + G I+P   VKV + +TG+   P+E GEL +K   +MK Y N  +     ++ +G+  SGDI YY  DG  Y VDRLK LIKYK   +AP E+E IL +HP + ++ V G  ++   EL +A VV++      E  +  FV+ +V+  K +RG + F DEIP+ S GK+ R+ +R  + +H+ +
Sbjct:   19 GTAGEQMFYALSRYADISG-CIALTNAHTKENVLYEEFLKLSCRLAESFKKYGLKQNDTIAVCSENGLQFFLPLIASLYLGIIAAPVSDKYIE-RELIHSLGIVKPRIIFCSKNTFQKV---LNVKSKLKYVETIIILDLNEDLGGYQCLNNFISQNSDINLDVKKF-KPNSFNRDDQVALVMFSSGTTGVSKGVMLTHKNIVARFSHCKDPTFGN-AIN---PTTAILTVIPFHHGFGMTTTLGYFTCGFRVALMHTFEEKLFLQSLQDYKVESTLLVPTLMAFFPKSALVEKYDLSH---------LKEIASGGAPLSKEIGEMVKKRFK-LNFVRQGYGLTETTSAVLITPDTDVRPGSTGKIVPFHAVKVVDPTTGKILGPNETGELYFKGDMIMKSYYNNEEATKAIINKDGWLRSGDIAYYDNDGHFYIVDRLKSLIKYKGYQVAPAEIEGILLQHPYIVDAGVTGIPDEAAGELPAAGVVVQTGKYLNEQIVQNFVSSQVSTAKWLRGGVKFLDEIPKGSTGKIDRKVLRQMFEKHKSK 544          

BLAST of predicted protein vs. nr
Match: gi|1325261085|ref|XP_023330441.1| (4-coumarate--CoA ligase 1-like [Eurytemora affinis])

HSP 1 Score: 226.483 bits (576), Expect = 1.190e-62
Identity = 161/563 (28.60%), Postives = 283/563 (50.27%), Query Frame = 0

Query:   70 VNKVKDRILTSGSEIKYQPQRIQGSLYQMVYRAMERYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGN-HNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVIC---MPDTQKLVTEALKLASRPQDIKTFCLGKNEE------FEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAIN--FGGPAINFLVPKTNVVTTTCFFHVGGF-FTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKG----VLNAYGQTESGIISA----GVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKH-----NDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWARH 606
            +N +KD+I+ S  E K  P++      + +   + + +++ GD     +  TG S T  +     +R+ +  L  G   GDV+     +  +  +P+ FG W+ GG +SLGD NL   ++ +QL   + + ++      D    V + LKL  +  ++K F L   E+       E    L+R  +  + P  +D   + +  +I WSSG++G PK I HS  + +N  FG    + L PKT ++++   FH+  F F      LK    Y M    F  ++LL+   K++P+ +  G   Y+Q+S        N  + + ++ + P G AV   C     EK  +L G    ++  YG TE   +S      +    LGS+   + + +++++TGER  P +EG++  KT  MM  YLNR K+  +F D +GFG +GDI YY   G I+F  R++E++K  N    P E+E++L++ P + E+ V+G++     ND+V      +V  + + + T+A++   V+  +   ++I G I F ++IP N  GK +R EM+++ + 
Sbjct:    1 MNVIKDKIIYSTLEPK--PEQPNQPYIERLLSEINQELDDTGDRDWLVDTETGSSRTISQIEPISRRVASALLSLGFGPGDVLQTGYSSCLDFYWPV-FGAWLCGGTVSLGDPNLSETNIKKQLEETKAKVLVSSIDYLDKYYKVIQELKLQGK--NVKYFILDAGEQDTLPLGVESFQTLMRVQETQKMPLLLDFTPDRE-SIIMWSSGSSGTPKGILHSQKNLLNLYFG----HDLKPKT-IISSALMFHISSFTFNLTLGILKGTICYIMKEKCFSGENLLKVAEKIQPEHVFCGVSQYIQVSN----TKTNGYNLNGVKNITPLGGAVSPGCS----EKVLSLLGSQATLVEMYGSTEVMFVSKHEVKDIEFGLLGSLAAGVEICIQDINTGERLDPGKEGKIMVKTNTMMMRYLNREKETNEFFDSDGFGFTGDIGYYDNFGKIFFSYRMREVLKVDNYWFGPAEIENLLEKEPTIDEACVWGEYDPNTGNDQVN---LGIVQKQNQKEWTKAKICSLVDANLPCTRKITGKIYFVEKIPHNPQGKKLRGEMKNFMKQ 541          

BLAST of predicted protein vs. nr
Match: gi|1176095348|ref|XP_020618234.1| (4-coumarate--CoA ligase 4-like [Orbicella faveolata])

HSP 1 Score: 228.409 bits (581), Expect = 2.766e-60
Identity = 164/516 (31.78%), Postives = 264/516 (51.16%), Query Frame = 0

Query:  105 RYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWI-LGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGG--PAINFLV--PKTNVVTTTCFFHVGGFFTGINACLKY-QTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFL-------APAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIISAGVSSQ-----ALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRD 602
            ++ +E  D +   + +T +S +  +     KR+ +   + G KKGDV  +LL N    FPI + G +  GG ++  +       +A+QL    T++++ +P       EA++   R      F +G  +  E L+ LL + D +  PD + ++ +ED+ V+ +SSGTTGHPK +  +H++ I+ G    +  FL     T ++    FFH  G    ++  L    T   M  P F  +  L A+   +   L +     + L+++        D+FD + +L       AP G  + Q+     K++   L  +   YG TE   +     +      +LG +LPN+R KV +V TGE     ++GE+C K P +MKGYLN P+  A  +D +G+ H+GDI +Y  D   Y VDR+KELIKY    + P ELE +L  HP ++++ V G  N EV EL  A VV K     TE ++  FV+  V  YK++RG + F DEIP++  GK++RRE++D
Sbjct:  566 QHFDEYEDREAMRDSITDRSYSYRQVKDLSKRIASALNKRGLKKGDVFAMLLPNLP-EFPIVYFGVLQAGGVITAANPLFTTEELAKQLKQADTKWILTVPQLVHRAKEAMQELGRSN---VFVIGDVDGCESLSGLLAD-DGSSFPD-VAINPKEDIAVLPFSSGTTGHPKGVILTHHNLISCGSIMRSKGFLSFDETTVLLAFLPFFHSYGMVAIMSMGLHCGSTIITM--PRFEREKFLHALQNYEVTHLPVVPPVVLMLAKHPSV-----DNFD-LSYLTEVTSGAAPLGKELSQAL----KDRLPLLSSIRQGYGMTELSPVCHMTPADNNKHGSLGVLLPNLRCKVVDVETGENLGHGQDGEICVKGPTVMKGYLNNPEATAQTIDSDGWLHTGDIGHYDEDEHFYIVDRVKELIKYNGYQVPPAELEALLISHPDIEDAAVIGIPNLEVGELPMAFVVRKANATLTEKDVEIFVDENVAPYKKLRGGVEFIDEIPKSLSGKILRRELKD 1063          

HSP 2 Score: 213.772 bits (543), Expect = 2.518e-55
Identity = 150/502 (29.88%), Postives = 252/502 (50.20%), Query Frame = 0

Query:  121 TGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWI-LGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKT-FCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAI--NFLVPK---TNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIIS-----AGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWARHQMRN 610
            +G+S T  +     K+  +   + G KKGDV  +LL N    FPI + G I +GG ++  +       +  QL       +I +P    L  + +K ++    I T + +G+ E  E L+ LL++ D  + P  ++++ +EDV  + +SSGTTG PK +  +H + I         +F+  K     V+    FFH  G    + +C  Y+    +  P F  +  L+ + + K    A+     + LS+          D  S++ +    A + +      KE+  +++     YG TE   +        +   ++G +LPN+  KV ++  G+  AP++EGE+C K P +MKGYLN P+  A  ++ +G+ H+GDI YY  D   + VDRLKELIKYK   +AP ELE +L  H A++++ V G  +    EL  A VV K   + TE E+ +++   V  +K +RG + F D+IPR+  GK++RRE+++     + N
Sbjct:   40 SGRSYTFEQLAVLTKKFASALTKKGFKKGDVFAILLPNIP-EFPIVYYGVIFIGGVVTTMNPLYTVEEITHQLQDAGVTSIITIP----LFADKVKESTSNVGINTVYVVGEAEGCESLSALLQD-DGEDFPKNVEINPKEDVACLPYSSGTTGVPKGVMLTHYNLIACALIVTHESFMWNKEEDAAVLGLLPFFHSFGQVISM-SCALYKGAKVVCVPKFEPESFLKIIQEHKVSHAALVPSTVLFLSKSPLVEQY---DLSSLKDVTSGAAPLGEELSKSLKERLPSIEWFRQGYGMTELSPVCHVTPFDNIKYGSVGVLLPNLECKVVDIENGDELAPNKEGEICVKGPTVMKGYLNNPEATAKTIESDGWLHTGDIGYYDEDEHFFIVDRLKELIKYKGFQVAPAELEALLVSHTAIEDAAVIGVPDARAGELPKAFVVKKANEEITEEEIAKYIEERVAPHKNLRGGVEFIDKIPRSLSGKILRRELKEKVTAALLN 531          

BLAST of predicted protein vs. nr
Match: gi|1228365368|ref|XP_021943573.1| (4-coumarate--CoA ligase 1-like isoform X1 [Folsomia candida])

HSP 1 Score: 221.476 bits (563), Expect = 3.641e-60
Identity = 178/622 (28.62%), Postives = 293/622 (47.11%), Query Frame = 0

Query:   17 SRTLAQGQSSRAASSSCLLIDLNVDIT---QFRPSKI-----MDNLVLELNCAPKYSKMFGVNKVKDRILTSGSEI----KYQPQRIQGSLYQMVYRAMERYIE--EDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTF-----CLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAINFLVPKTNVVTTTCFF---HVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIISAGVS---------------SQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDN-ADFLDFEG--FGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFG-KHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMR 597
            SRTL+       A SS  ++ +N  IT   +F P  I       +LV ++     + + F      + I+ +G EI     Y P ++      M    ++R  E  ++G  K   N +TG+++T  +     +++ +F ++ G K GD I  L  +    F I  G W  GG +          ++++++     +++ C P T      A K  S P +I  F     C   +E FED        D  +CP+ +  D+ + + +I  +SGTTG  K   +S+ + +NF        VP+ N +         HV G    +     +   + +   +   +++ +A+ K  P  L  G   ++     D   +AN  D  SIE +   G  +  S     K K  N+K V+N +G TE G I+  V                + ++G + PN ++KV+++ TG    P ++GELC  +P +  GY NRP+ N  +F +  G  + ++GD+ YY  +G ++ VDR+KE  KY NNHI+P ELE+ +  HPAV E+ VFG K  D    +  A+VVLK    AT  E+  F N +V  YK++RG +IF D++PR   GK++R
Sbjct:   10 SRTLS-------AVSSSFIVRVNTPITSYTKFWPRSISVAIKKPDLVSQILLVQSH-RNFHKQSCNNMIVKNGGEIILESDYVPNKLVYKHKSMAQALLDRAEENLKEGRSKWMTNSITGETLTFNDVDVMSRKVASFLVKRGMKSGDKIIYLTFDITKMFAIVIGVWRTGGVVCTSYPEDTAETLSDRVKDYTAKWLFCDPSTVAQCQSAAKTVSWPVEICVFGTAAGCTSVDEIFED--------DGAQCPEKITSDLHDPI-LILCTSGTTGKSKGAIYSNFTVLNFCSATDG--VPRHNPLPALWLLKGTHVLGLLYPLRNV--FVGDHSIMMTTINKENIFKAVDKYHP-FLVFGFPLFLISLVND--PEANKIDRSSIEIVCSGGIVLKDSFYETMK-KLPNVKYVINGFGMTECGAITTTVDIGGSVDLIRAVPNIPTLSVGKLYPNTKLKVKDLKTGTPLGPSKQGELCVSSPILSSGYWNRPEVNEQNFQEVGGDRWMNTGDLGYYDENGFVFVVDRIKETFKYFNNHISPAELEEYILLHPAVGEACVFGMKDPDGGDHIPRAIVVLKAGMSATPEEIASFTNGKVAGYKQLRGGVIFVDQLPRGKTGKVLR 606          

BLAST of predicted protein vs. nr
Match: gi|1176094477|ref|XP_020617773.1| (4-coumarate--CoA ligase 1-like [Orbicella faveolata])

HSP 1 Score: 218.009 bits (554), Expect = 1.158e-59
Identity = 157/526 (29.85%), Postives = 262/526 (49.81%), Query Frame = 0

Query:   97 QMVYRAMERYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICF-GGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQ-DIKTFCLGKNEEFED---LNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAIN----FGGPAINFLVPKTNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLK--------PKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIISAGVSSQ----ALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMR 601
            + VY+  ++Y    GD     +  +G + T  +  T  K   +  ++ G KKGDV+ + L N    +PI F G   +GG ++  +       +  QL   Q+ F++  P    L  +  + AS+ Q  IK   +  +E  ED    ++LL + D +  P    +D ++DV  + +SSGTTG PK +  +HN+ I       G  I  L  ++ V+T   FFH+ G    + + L +Q    +    F  +  L+AM   K        P  L +  H  V     D +      D  S+E +    A + +  E    ++   +K +   YG TE  +       +    ++G +L N+  K+ +V+TG    PH++GE+C K P +MKGYLN P+  A  +D EG+  +GDI +Y  DG  + VDRLKELIKY    +AP ELE +L  H  + ++ V G  +++  EL  A VV  PK + +  ++++FV   V  +K++RG + F D+IP+++ GK++R+++R
Sbjct:   20 EFVYQNFDKY----GDTTAIIDGTSGHAYTFTQLKTLTKNFASSLVKRGFKKGDVLAIYLPNVP-EYPIAFYGAGFIGGTVTTVNPLYTTEELERQLQDTQSSFLVTSP----LFIDKARAASKAQGKIKKIIVIGDEVSEDCLAFSNLLED-DGSSFPSQCTVD-KDDVVSMPYSSGTTGVPKGVMITHNNMIADTCIMEGSEIFTLTEQSVVMTILPFFHIYGQLVMMGSGL-HQGAKLVVQQRFEPEKFLKAMQDHKVTNAPIVPPIVLFLAKHPLV-----DKY------DLSSVEEVTCGAAPMGEGLEEALIKRMPRVKRIRQGYGMTEVLVTHVQPRGERKPGSVGVLLSNVESKIVDVTTGAMLGPHQDGEICIKAPTVMKGYLNNPEATARMIDSEGWARTGDIGHYDEDGHFFIVDRLKELIKYNAYQVAPAELEALLISHLNIDDAAVIGVPDEKSGELPKAFVV--PKGEISPEDVVDFVAERVAPHKKLRGGVEFVDKIPKSASGKILRKDLR 520          

BLAST of predicted protein vs. nr
Match: gi|1126184687|ref|XP_019624977.1| (PREDICTED: 4-coumarate--CoA ligase 1-like [Branchiostoma belcheri])

HSP 1 Score: 219.935 bits (559), Expect = 1.178e-59
Identity = 152/516 (29.46%), Postives = 264/516 (51.16%), Query Frame = 0

Query:  103 MERYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASR--PQDIKTFCLGKN-EEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAINFLVPKTN-----VVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIIS-----AGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWAR 605
            + ++ ++ G+     N +TG+S T ++     +R G+   + G K+ DV+ +   N        FG   +GG ++  +    P  +A QL     ++VI +PD    V E +K A    P   + F +G +  E  + +DLL + D +  P+ + ++  EDV V+ +SSGTTG PK +  ++N+ +      IN  + + N     ++    FFH+ G    ++ CL+ Q    +  P F  +  LR +   K   + +     + LS++         D   ++ L  A A +  +     ++K +  + +   YG TE+  ++        +  ++G  LPN  VKV +  TG+     E+GE+C + P +MKGYLN P+  A  +D +G+ H+GDI +Y   G  Y VDRLKELIKYK   +AP +LE +L  HP+VQ+  V G  ++   E+  A +V K     TE E++++V  +V  +K++RG + F +EI +++ GK++RR +RD +R
Sbjct:   95 VTQHFDDYGNDVAITNGVTGESYTYLQLQDLIRRFGSALTRLGFKQHDVLAIFSPNVPEYAIAFFGASSVGGVVTTANPTYTPDELAYQLQHSNAQYVITIPD----VAETVKAAKSKCPSVKEIFVIGSDVPECRNFSDLLAD-DGSAFPENVSVNTAEDVAVLPYSSGTTGRPKGVVLTNNNIVANLRQIINKGMLEFNRQEDCLIAQLPFFHIYGMVAVLSCCLR-QGVRFVTIPRFEPELYLRVIQDYKVNRVMMVPPIALFLSQHPMVDQY---DLSRVKDLMCAAAPMGINLTMALRDKLKP-QSLRQGYGLTETSPVTHLCVEDEFAPGSVGITLPNTEVKVIHTETGQALGEGEDGEICVRGPQVMKGYLNNPEATAGCIDSDGWFHTGDIGHYDDKGYFYIVDRLKELIKYKGLQVAPADLEAVLLGHPSVQDVAVIGVPDERAGEVPKAFIV-KKSDDVTEKEIVDYVAGKVAPFKKLRGGVEFVNEISKSASGKILRRTLRDMSR 599          

BLAST of predicted protein vs. nr
Match: gi|1005487375|ref|XP_015780279.1| (PREDICTED: 4-coumarate--CoA ligase-like 7 [Acropora digitifera])

HSP 1 Score: 225.713 bits (574), Expect = 1.318e-59
Identity = 160/519 (30.83%), Postives = 256/519 (49.33%), Query Frame = 0

Query:  105 RYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWI-LGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGG--PAINFLV--PKTNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSK--------LKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNL---KGVLNAYGQTESGIISAGVSSQ-----ALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRD 602
            ++ +E GD     + +T +S +  E     ++  +   + G +KGDV  + L N    FPI F G +  GG ++  +       +A+QL     ++++ +P       EA++   R        +G+ + +E +++LL++ D +  P+P  ++ +ED  VI +SSGTTGHPK +  +H + I  G    A  FL     T ++    FFH  G    ++  L   ++  +  P F  +  L+ + K        + P  L +  H  V             D++D          A P   E I KE  + L     V   YG TE   +      +     ++G +LPN+R KV ++ TGE   P  +GE+C K P +MKGYLN P+  A  +D +G+  +GD+ +Y  D   + VDR+KELIKYK   + P ELE +L  HP +Q++ V G  + E  EL  A VV KP    TE ++  FV+  V  YK++RG + F DEIPR+  GK++RRE++D
Sbjct:  443 QHFDEYGDRMAIEDCVTKRSYSFREIKVLCRKCASGLSKKGFQKGDVFALFLPNLP-EFPIAFFGVLQAGGVVTSANPLFTTEELAKQLKQADAKWILTVPQLVHRAKEAMQELGRDN---VLVVGEVDGYESMSELLKD-DGSSVPEP-SINPKEDTAVIPFSSGTTGHPKGVMLTHYNLIACGSMMRAQGFLSFDETTVLLAFLPFFHSFGMVANMSMGLHTGSFL-VSMPRFDKEKFLQVLEKYEVTHSSVVPPVVLLLAKHPAV-------------DNYDLSNLTEITNGAAPLG-EEISKEVMKRLPLLSRVRQGYGMTELSPVCHMTPFENNKLGSVGMLLPNLRCKVIDLETGEALGPGHDGEICVKGPTVMKGYLNNPEATAQTIDSDGWLCTGDVGHYDEDEHFFIVDRVKELIKYKGYQVPPAELEALLVSHPDIQDAAVVGVPDVEAGELPMAFVVRKPNAALTERDVKTFVDENVAPYKKLRGGVEFIDEIPRSLSGKILRRELKD 940          

HSP 2 Score: 175.637 bits (444), Expect = 1.785e-42
Identity = 116/396 (29.29%), Postives = 193/396 (48.74%), Query Frame = 0

Query:  247 TECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAINFLVPKTN---------VVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSE---YDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIISAG-----VSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMRDWARHQMRNQEQDFHRPIFHERMA 625
            +  P  ++++ ++D+  + +SSGT G PK +  +H + I     A   LV   +         V+    FFH  G    + +C   +    +  P F  +  L+ M   K    A+     + L++    D F      D  S+E ++   A + +       E+  ++K     YG TE   +S       V   ++G +LPN+  KV NV  G    P+++GE+C + P +MKGYLN  +  A  +D +G+ H+GDI +Y  D   Y  DRLKELIK K   + P ELE++L  HPA+ ++ V G  + E  EL  A VV K     T+ +L++F+   V  YK + G + F DE+PR+  GK++RR++++     ++  ++       HE +A
Sbjct:   22 SNLPKDVEINPKDDIACLAYSSGTMGSPKGVMLTHYNLI-----ADALLVTHESFLWMNEHPVVLGLLPFFHAFGQIN-VLSCPLLKGGTLVCVPKFEPESFLKIMQDFKVTHAALVPPTVLFLAKSPLIDQF------DLSSLEDVSSGAAPLGEDLAKTLTERIPSIKWFRQGYGMTELSPVSHATPCDQVKYGSVGVLLPNLECKVINVEDGTELGPNKDGEICVRGPTVMKGYLNNQEATAQMIDSDGWLHTGDIGHYDQDEHFYITDRLKELIKCKGYQVPPAELENLLVSHPAITDAGVIGVPDSEAGELPKAYVVRKANENITDQDLVKFIEERVAPYKALGGGVQFIDEMPRSLSGKILRRKLKEMEEANVKQVQE-------HENLA 398          

BLAST of predicted protein vs. nr
Match: gi|1325285932|ref|XP_023327697.1| (4-coumarate--CoA ligase-like [Eurytemora affinis])

HSP 1 Score: 218.009 bits (554), Expect = 1.421e-59
Identity = 152/545 (27.89%), Postives = 258/545 (47.34%), Query Frame = 0

Query:   70 VNKVKDRILTSGSEIKYQP--QRIQGSLYQMVYRAMERYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGN-HNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEA---LKLASRPQDIKTFCLGKNEEFEDLNDLLRNI---DPTECPDPMDMD--VEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAINFLVPKTNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFR-LDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIISAGVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQE-LVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMR 601
            +++++D++L S       P  +RI   L  ++    +  + E GD     +  T  S+     L    ++ +   + G K GD +H       +  +PI FG W+ GG +S+GD  L   ++  Q+     + ++C  ++     EA   L  A +  +I    L   +   +     R++      +CPD   +   + ++  VI W+SGTTG PK +  S    ++F     + L  KT  + +  FFH G F   ++  ++ +   H   P    ++ + R + K KP     G++ +VQL+      D    D  S+  + PAG  + +S     KEK  NLK V   YG TE G +S   + + +G++   ++  + +  TGE+    E GE+  KTP MM  YLNR  ++ + LD EGF H GD+ YY  +G +++ +R+KE+IK  N  I P E ED L+  P V E++V+   N E     +   V L+        +++E  N++V   +RI G I   + +P N  GK +RR +R
Sbjct:    1 MSEIRDKVLYSKEPYTLDPGQERIIFKLLDLI----QAELRETGDRDWILDCTTENSVRISHILGRSIKVASGLTRLGFKPGDTLHTAYSTCLDFYWPI-FGAWLCGGVVSVGDPGLSASNLKSQIQDTHAKIIVCEVESCSKFLEANQSLLSAEQASNILVLDLEPWQPIPEGTSSFRSLYEDQGDQCPDRASLPGYIPKEPVVIHWTSGTTGSPKGVVMSQE-YLHFQMRQ-SKLPEKTIGLASNTFFHAGSFLLSLDGGIRNKFSIHFIQPKHTTVESIFRFIEKYKPMFFMCGANDFVQLAS----SDLAKFDLSSVNVIMPAGNKIAKSAATRMKEKFPNLKFVYQFYGSTEVGGVSGSFTVENMGALREGVQTYIRDQKTGEKLGVGEVGEIMAKTPTMMIHYLNRQDESRELLDEEGFAHMGDLGYYDENGILHYKERIKEMIKIGNYWIGPAEFEDNLENIPGVVEAVVWQSMNKETDNYYIHGAVTLQASSGLNPEKILELANKDVEVKRRIDGTIHIINNMPHNPQGKKLRRTVR 534          

BLAST of predicted protein vs. nr
Match: gi|1176095425|ref|XP_020618275.1| (4-coumarate--CoA ligase 2-like [Orbicella faveolata])

HSP 1 Score: 220.32 bits (560), Expect = 1.540e-59
Identity = 155/508 (30.51%), Postives = 248/508 (48.82%), Query Frame = 0

Query:  120 LTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWI-LGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIKTFCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSHNSAINFGGPAINFLVPKTNVVTTTCFFHVGGFFTGI--NACLKYQTYYHMFG------------------PSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGVLNAYGQTESGIISA-----GVSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKLMRREMR 601
            ++ KS T  E  T  K   +   + G KKGDV  + L N    +PI F G I LGG  +  +     + +A QL     +++I +P    +  +A + A+     + F +G+ E  E L  LL + D T  PD + ++ +EDV V+ +SSGTTG PK +  +H+                 N+V+  C      F +       L    ++H+FG                    F  DH+L+ +   K   L +       L+++    +    D  SI+ +    A + +      ++   ++  +   YG TE+   +           A+G +LPN+  KV +V TGE   P+++GE+C + P +MKGYLN  K  A+ +  +G+ HSGD  YY  +G  Y VDRLKELIKYK   +AP ELE +L  HP + +  V G  +DE  E+  A +V  PK   TE E+I+FV   V+ +K++RG + F ++IP+ + GK+++RE+R
Sbjct:  167 VSNKSYTYSELSTSVKNFASAVAKRGFKKGDVFALYLPNVP-EYPILFFGIIALGGVATTLNPTFTGKEIAYQLKDSGAKYIITVP---AIADKAKQAAAEVGINQVFVIGEAEGCEPLTSLLSD-DGTAFPDGVPINPKEDVCVMPYSSGTTGFPKGVMLTHH-----------------NLVSQLCMIMHNSFRSHPLRGTVLGLLPFFHIFGMMVVMVQFLQRGGKIVCMQQFDGDHMLKLIQDFKIHCLYLVPPIITFLAKHPSVKNY---DLTSIDTIISGAAPLGKELTHAVRKNLPSVVALGQGYGLTETSPAAMLCPRNSCKPGAVGVMLPNLEGKVVDVQTGELLGPNQDGEVCMRGPTVMKGYLNNAKATAESITSDGWFHSGDTGYYDEEGHFYIVDRLKELIKYKGFQVAPAELEALLHTHPNILDVAVVGIPDDEAGEIPKAFIV--PKGDITEKEIIDFVADNVSPHKKLRGGVEFLEQIPKTASGKILKRELR 647          

BLAST of predicted protein vs. nr
Match: gi|973401421|ref|WP_059143627.1| (MULTISPECIES: 4-coumarate--CoA ligase family protein [Streptomyces] >gi|973302779|gb|KUL63246.1| AMP-dependent synthetase [Streptomyces violaceusniger])

HSP 1 Score: 217.624 bits (553), Expect = 1.654e-59
Identity = 153/511 (29.94%), Postives = 246/511 (48.14%), Query Frame = 0

Query:  103 MERYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIK-TFCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSH-NSAINFGGPAINFLVPKT---NVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGV---LNAYGQTESGIISAG----------VSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKL 595
            + R  EE GD     + +TG +++        +R+GA   + G +KGDV+ +   N  M  P  +G    G  ++       P   A+QL      +VI +     L+  A + A R   ++  F   +      L  ++R+  P      +D+D  ED+ V+ +SSGTTG PK +  +H N + N     +  LVP      V+    FFH  G    +NA L+      +  P F LD  L A+ K + + L +     + L+++      +  D  S+ ++  A A +    +A   E C    GV   L A+G TE   +S G               +G +LP+  +++    TGE  A  E+GE+  + P +MKGYL RP+D    +D EG+ H+GD+ +   DG +Y VDR+KELIKYK   +AP +LE +L  H AV ++ V G  +    E+  A VV +P  + +E +LI +V  +V  YK++R  + F D +PR++ GK+
Sbjct:   22 LARAAEEFGDLTALVDGVTGAALSYAALDRAARRIGAALAEAGVRKGDVVALHSPNSIMYPPAFYGATRTGAAVTTVHPLATPEEFAKQLRDAAASWVITV---SALLGVARQAAERAGGVREIFVCDEASGHRSLRSMMRSTAPEPV---VDIDPAEDIAVLPYSSGTTGTPKGVMLTHRNVSTNLA--QVETLVPNQPGERVLAVLPFFHSYGLTALMNAPLRNGGTVIVL-PRFELDTFLAAIEKHRAQALYVAPPIVLALAKHPSV---DGHDLSSVRYVLSAAAPL----DARLAEACARRLGVPPLLQAFGMTE---LSPGCHLVPRDAKNAPPGTVGKLLPSTEMRIVAPDTGEDLAVGEDGEIVIRGPQVMKGYLGRPEDTDALIDAEGWLHTGDVGHVDADGWLYVVDRVKELIKYKGYQVAPADLEAVLLAHEAVADAAVIGVTDGTGNEIPKAYVVRRPGTRISEDDLIAYVAGQVAPYKKVR-RVEFTDTVPRSATGKI 512          

BLAST of predicted protein vs. nr
Match: gi|1268739934|ref|WP_099014090.1| (4-coumarate--CoA ligase family protein [Streptomyces malaysiensis] >gi|1266920601|gb|ATL83639.1| 4-coumarate:CoA ligase [Streptomyces malaysiensis])

HSP 1 Score: 217.238 bits (552), Expect = 2.011e-59
Identity = 154/511 (30.14%), Postives = 245/511 (47.95%), Query Frame = 0

Query:  103 MERYIEEDGDWKVFNNLLTGKSMTAIETLTYGKRLGAFFLQNGCKKGDVIHVLLGNHNMTFPICFGGWILGGCMSLGDVNLEPRSVAEQLNTCQTRFVICMPDTQKLVTEALKLASRPQDIK-TFCLGKNEEFEDLNDLLRNIDPTECPDPMDMDVEEDVPVIFWSSGTTGHPKAICHSH-NSAINFGGPAINFLVPK---TNVVTTTCFFHVGGFFTGINACLKYQTYYHMFGPSFRLDHLLRAMSKLKPKTLAIGSHHYVQLSEYDGFHDANPDDFDSIEFLAPAGAAVPQSCEAIYKEKCRNLKGV---LNAYGQTESGIISAG----------VSSQALGSILPNIRVKVENVSTGERCAPHEEGELCYKTPAMMKGYLNRPKDNADFLDFEGFGHSGDIVYYTPDGDIYFVDRLKELIKYKNNHIAPTELEDILQRHPAVQESLVFGKHNDEVQELVSAVVVLKPKHQATEAELIEFVNREVTDYKRIRGDIIFRDEIPRNSVGKL 595
            + R  EE GD     + +TG +++  E     +R+GA   + G +KGDV+ +   N  M  P  +G    G  ++       P   A+QL      +VI +     L+  A + A R   I+  F   +      L  +L +  P      +++D  ED+ V+ +SSGTTG PK +  +H N A N     +  LVP      V+    FFH  G    +NA L+      +  P F LD  L A+ K + + L +     + L+++      +  D  S+ ++  A A      +A   E C    GV   L A+G TE   +S G               +G +LP+  +++ +  TGE  A  E+GE+  + P +MKGYL RP+D    +D EG+ H+GD+ +   DG +Y VDR+KELIKYK   +AP +LE +L  H AV ++ V G  +    E+  A VV +P  + +  +LI +V  +V  YK++R  + F D +PR++ GK+
Sbjct:   22 LARAAEEFGDLTALVDGVTGAALSYAELDRASRRIGAALAEAGVRKGDVVALHSPNSIMYPPAFYGATRTGAAVTTVHPLATPEEFAKQLRDAAASWVITV---SALLGVARQAAERAGGIREIFVCDEASGHRSLRSMLGSTAPEPV---VEIDPAEDIAVLPYSSGTTGTPKGVMLTHRNVATNLA--QVETLVPSRPGERVLAVLPFFHSYGLTALMNAPLRNGGTVIVL-PRFELDTFLAAIEKHRAQALYVAPPIVLALAKHPAV---DGHDLSSVRYVLSAAA----PLDARLAEACARRLGVPPLLQAFGMTE---LSPGCHLVPRDAKNAPPGTVGKLLPSTEMRIVDPETGEDLAVGEDGEIVIRGPQVMKGYLGRPEDTDALIDAEGWLHTGDVGHVDADGWLYVVDRVKELIKYKGYQVAPADLEAVLLAHEAVADAAVIGVTDGAGNEIPKAYVVPRPGTRVSADDLIAYVAGQVAPYKKVR-RVEFTDAVPRSATGKI 512