e3 ubiquitin-protein ligase hyd, maker-scaffold114_size351134-snap-gene-1.12 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Match: EMLSAG00000000248 (supercontig:LSalAtl2s:LSalAtl2s1036:68871:78712:-1 gene:EMLSAG00000000248 transcript:EMLSAT00000000248 description:"maker-LSalAtl2s1036-augustus-gene-0.34") HSP 1 Score: 2347.01 bits (6081), Expect = 0.000e+0 Identity = 1280/2102 (60.89%), Postives = 1538/2102 (73.17%), Query Frame = 0
Query: 82 GIKRTLAELESDPTSPNTIETVQRILEERCDGGRNIFHTAVSMCQPTSNKDPDVQ---DQSAASMSSSSGLDSMDAMSAAYASKAMNLRDMMRRATRLELPSSVGGSQAGPSASTSAPLAPSVAAAAAAAAAVASGGVAPPGDASPPVIEEPSLPSLPWPPEPMEGNSGDEDSLLGLAGSAA-SISSHKSVLVKERSPATENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKSTSRKT--PELDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQVGSDKGEARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSL-SPSNDSVSIEELFSVEPMVPR-NSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEVNDDGP--VENPDQDDQHSDM-DLDLLAETDSESDDGEPNDRDAKSGGDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQ-ERSNLAPQSMQWALRSRSKSSRTVNGG------FIYIDPNALRRTTSSGTAVAT---PNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTPGRTRTGFQSRA--------GSLASGQ--DTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRSGTDAQEDSP-FGMD--PTPMVPSAASNFDENENDDEAAGSGTPATNMEVDDEDTNQSAAILAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMTNTLAVLPTRLGLSARTTDEAGATSAVQPAE------AFNA----MSTAPFQATPITGDSNFENVVRQARP--SSPTAATCDTASVRSLDTTVTGGNSEEAETDQPQDLSMGGGGTMSSASSVSEEP----VASTSKGSFTSPKKAFMMREAAR--------GASSA---PEVLVVPTEKPDQGDVSANVTIETSAARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQ-NRFYGRRSRDAARALNFEARPFYLNGVGGSNDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGS-----SGSSAAAAGVAGQEHLLPDLDVF---------------------NLGNSSGVKRKSLSGSVAL------GSEPELQEE-ENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
G+KR ++ LE+DP S N++ETVQ ILEERCDGGRNI HT VSMCQPTSNKDPD+ + +S S S+ L+S++++++A +S+AMNLRDM +A S+ AA + + A G A G SPPV+++ + P+L WP + +E SGD+ SLL LA S+ S++SH+ + + EN ERR+NAL+ LK IC S V PHLE++L+MQDAQ NTP MA+I RAYPAALVLF+ A K+A + SND T KKTLMSMVFP G+ PD+SPLHVICCNDTCSFTWTG +HINQDIFECRTCGLTDSLCCCTECARVCHKGHD KLK+TSPTAYCDCWEKCKCRSLIGG+Q AR+EVL +LI+DT+LV N RGENILLFLVQTVGRQ EQRQ+ PSR RKS SRKT E++ D PEHDL+PPRF RRAL+ LL DW A+K+MIMTGH P G + P+YEDQAFLASQSGT LLDKF HCL+V+C+G+ LD LLT+LIR +Q K EA+ VAKRFVRSVTRVFVVFN+EM+P+Q+KKK+LQ+ +QPL RC+RVFQALINIA+EELCETANALLAPVR+G++RPTAPF+L S SN+ VS EE+FSVEP+ PR NS S +RS++SSD++ + S+ S ++ D E ++ EDNED GE++ D ++ + P +ENPD DDQHSDM DLDLLAE++S+S++ + ++ +++ G +ALFSDD+S +S+HP+D ESDAGETDEQD D TF +EQLERR T GT+Q ERSNL P SMQWA+RSR K SR+ G FIYIDP+ALRRT SS T P E +TM TTAS+LAR+FGIVIRQIADLLTMLQDYN++AP LARTLEISYQESI+LQL IEY MKPNWDWLM V+DSTEAQLRFGSAL TD++ +PN+P R+ R+ S A+ Q + +NRRDFLNYALSLMRAHN+EHSDSLPV+DV+++KHIAYVFDALIYYMRSG+D+ EDS F +D + + +P+ DDE +N + + + K RRH FFQRSDSTLCLGC PPDPFHT MQEALPLADQP LLQPNARREDLFG+PKQP+ +S N L+VLPTRLGLS R +D A A ++ NA +STAPFQA P ++NFE +V QAR +SPT ATCD+AS++SLDTT T + E ++PQDLSMG + SS + + E + FTSPKKAFMMREAAR G+ S+ PEVLVV T+ + + VT+ETS +RV +LGVSVPHDILLGRWR LDLFGRVF+D+VG+EPGSIISELGGFP KE KFRREMEKLRN RT D I+KIERERDQLI QAFKEFN+ NQ+ RRSSSSQPPLVV RVKVTFQNEPGEGSGVARSFYTAL+EALL++ KLPNLE AQ M F L+QRLRG+RD + +RR+ ++ Y +S R L+++ARPFY+NG GGSN+HLS HQ QLG+RL+ RVQ LRP L++KITGML ELSPAQLLLLLASE+S RQRV+ A ++I + N +E + SG +A +GG S + +GSS++ + VA E++LP+LDVF S V S+SG + G E+++ E+ ED+APLFY PGKRGFYSP+QG+ T ER+NAFRNVGRL+GLCLLQNELCPLF NRHVIKY+LGR+IRFHDLAFFDPVIYESLRQLVVDAENK+TSAQLFSAL+L FSIDLS EEGGGS EL+PNGR++E A NVY YVR+YS YRM CQEKAL N+K+GL+DVLPAG+LDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGE+ +RLVNFKRWLW +VEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPS+TIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLL+AIKTKNFGFV
Sbjct: 953 GVKRIISSLEADPASINSLETVQSILEERCDGGRNIIHTIVSMCQPTSNKDPDMDPNSSSTTSSNSISARLESIESIASAVSSRAMNLRDM--------------------XRRATAAATRSIEAAGGGSGSSAVLGDAAGG--SPPVLDD-AXPNLAWPSDSLEAASGDDXSLLSLANSSNKSLASHQK---EPKVVTNENVERRANALSILKTICGSPVLFPHLESMLSMQDAQGNTPLMASIANRAYPAALVLFDTANKIANESSNDEATVKKTLMSMVFPDGASPDSSPLHVICCNDTCSFTWTGEDHINQDIFECRTCGLTDSLCCCTECARVCHKGHDFKLKRTSPTAYCDCWEKCKCRSLIGGNQEARFEVLGRLISDTNLVTLPNSRGENILLFLVQTVGRQATEQRQFHPSRPRKSASRKTVVSEIENDRPEHDLEPPRFCRRALEKLLSDWTAVKSMIMTGHL-PVPNGSNSFPIYEDQAFLASQSGTALLDKFVHCLIVKCTGERLDTLLTSLIRHIQHSDAKSEAKIVAKRFVRSVTRVFVVFNVEMSPAQTKKKALQSATQPLQRCKRVFQALINIAVEELCETANALLAPVRYGVARPTAPFNLTSGSNEFVSAEEIFSVEPLAPRTNSNSNGRSRTRSLISSDNNNSNLPRASRRQGSNSGNSNNATNQDNFESNEAEDNEDVGDEGEESMDQVEAIEGIEEPSVLENPDGDDQHSDMMDLDLLAESESDSENEQESNAPNNDNQNSDNVVGSDALFSDDESADSSHPDDDESDAGETDEQDGEDLTFVDEQLERR-TNGTSQDERSNLTPLSMQWAIRSRPKGSRSSGNGSSSGGGFIYIDPSALRRTNSSSXTAGTTPTPANIEPVTMSTTASALARSFGIVIRQIADLLTMLQDYNAIAPALARTLEISYQESISLQLYIEYQMKPNWDWLMTVLDSTEAQLRFGSAL---TDISDPSHPNHPLHSSPRSSSHGRSERNNNIYHASRAATQNPEMLNNRRDFLNYALSLMRAHNSEHSDSLPVIDVSALKHIAYVFDALIYYMRSGSDSVEDSNRFNLDYDENENDEVEEAAGTXSATTTNIEMESSPSPPRGDDDETSNTTGQV--TVSGKERRHPFFQRSDSTLCLGCAPPDPFHTCMQEALPLADQPQLLQPNARREDLFGIPKQPLN------SSSNNPLSVLPTRLGLSVRNSDAASAVASNSNNGSSNSHNVTNAGNLELSTAPFQAGP-NAEANFERLV-QARSXNASPTXATCDSASIKSLDTTATTMDLE----NEPQDLSMGAMSSASSVTGDTVESGSSSSQLLQQQRFTSPKKAFMMREAARENLERDKGGSGSSLLPPEVLVVSTDAQTKPEQDGEVTVETSR-QRVTNLGVSVPHDILLGRWRLTLDLFGRVFIDNVGIEPGSIISELGGFPHKEEKFRREMEKLRNCRTQDFVISKIERERDQLIVQAFKEFNALYNQNQRRSSSSQPPLVVGRVKVTFQNEPGEGSGVARSFYTALAEALLANKKLPNLEPAQ------MPFNLVQRLRGTRDNRTERRLAVHKSYTYKSNRDVRPLSYDARPFYVNGEGGSNEHLSPHQHQLGDRLYQRVQVLRPSLSSKITGMLXELSPAQLLLLLASEDSXRQRVDXATDIILT---NTNEQNTQGHSGGVAPTTTSGGSASSSIAGSTTSQQQQQPLNTVAGSSSSTSTVAPHENILPELDVFSLSASSNSSTSTPSVVASVAAGTAAXSNVNTSSISGVATVKKKRIPGGTVEVEDSIEDSEDSAPLFYCPGKRGFYSPVQGKATQERLNAFRNVGRLMGLCLLQNELCPLFLNRHVIKYILGRTIRFHDLAFFDPVIYESLRQLVVDAENKETSAQLFSALDLNFSIDLSPEEGGGSVELIPNGREVEXXANNVYCYVRRYSYYRMIKCQEKALSNIKIGLYDVLPAGALDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGESQDRLVNFKRWLWQMVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSITIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLLAIKTKNFGFV 2999
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Match: EMLSAG00000004991 (supercontig:LSalAtl2s:LSalAtl2s260:299952:311628:1 gene:EMLSAG00000004991 transcript:EMLSAT00000004991 description:"maker-LSalAtl2s260-augustus-gene-3.39") HSP 1 Score: 114.775 bits (286), Expect = 2.208e-25 Identity = 80/299 (26.76%), Postives = 142/299 (47.49%), Query Frame = 0
Query: 1792 LFYT-PGKRGFYSPIQGRGT-PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSA--ELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALP----ASEDGFQPMPSVTIRPAGDS--HLPSANTCISRLYVPLYSSKAILKSKLLMAI 2080
LF T PG + Y + T P ++ F+ VGR++ + N+L +F R K++LG+ +++ D+ D Y+ L + +V+ + D +LTFS ++ +E G + +L+PNGR+I V +Y+R Q +M K L G ++++P + ++ LLL+G+ I++ L S T ++ + + + W W + +K + F TGS +P A +G + I S LPSA+TC ++L +P Y + L++ LL AI
Sbjct: 2664 LFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGL-EFLVNHKVSDL------GYDLTFSTEI--QEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQII-----WFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLEGMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAI 2948
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Match: EMLSAG00000006171 (supercontig:LSalAtl2s:LSalAtl2s336:651240:657086:1 gene:EMLSAG00000006171 transcript:EMLSAT00000006171 description:"maker-LSalAtl2s336-augustus-gene-6.36") HSP 1 Score: 112.079 bits (279), Expect = 8.982e-25 Identity = 75/283 (26.50%), Postives = 129/283 (45.58%), Query Frame = 0
Query: 1811 PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSH------------LPSANTCISRLYVPLYSSKAILKSKLLMAIK 2081
P+ ++ F VGR++G+ + F K +L + I D+ DP +++SL+ ++ S + ++ TFS++ EL NG+DI VN +N YV+ Y Q+R E+ +++ G +++P L ++ L++ G+G I++ ++T ETP +W W IV+ S + L+ F TGS +P GF+ + T + G H LP A TC +RL +P Y S I+ KL A++
Sbjct: 658 PDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWIL--------SNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETP-----VVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQ--GFKDLQGSTGQ-TGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVE 924
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Match: EMLSAG00000007514 (supercontig:LSalAtl2s:LSalAtl2s431:6204:41118:1 gene:EMLSAG00000007514 transcript:EMLSAT00000007514 description:"maker-LSalAtl2s431-snap-gene-0.13") HSP 1 Score: 100.523 bits (249), Expect = 2.265e-21 Identity = 78/289 (26.99%), Postives = 134/289 (46.37%), Query Frame = 0
Query: 1803 SPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSA---ELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPS------VTIRPAGD-SHLPSANTCISRLYVPLYSSKAILKSKLLMAIK 2081
+P G + ++ F+ +GR G+ +L FF R K +LG+ + D+ D + SL + ++ Q LELTF +D EE G+ EL P G++I V N Y+ + Q+R S +K ++N G +++P + + LL+ G+G I+V+ T + + P +V +W W +V + + L+ F TG+ +P +GF+ + TI G+ S P A+TC +RL +P Y S L+ +L+ AI+
Sbjct: 473 NPYSGVCNEDHLSYFKFIGRXAGMAXXXGKLLDGFFIRPFYKMMLGKGMELSDMESVDTEXFNSLMWI------RENDPQ---ELELTFQVD---EENFGTTVTRELKPGGKNIPVTNENKDEYIHQVIQWRFVSRIQKQMDNFLAGFNELVPLSFIKIFDEGELELLMCGIGSIDVKDWKQNTVYKGDY--HPNHIV--IQWYWRVVLSFNNEMRSRLLQFVTGTSRVPM--NGFKELYGSNGPQLFTIERWGNPSSYPRAHTCFNRLDLPPYESYQQLRDRLIKAIE 743
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Match: EMLSAG00000011039 (supercontig:LSalAtl2s:LSalAtl2s744:109377:113126:-1 gene:EMLSAG00000011039 transcript:EMLSAT00000011039 description:"maker-LSalAtl2s744-snap-gene-1.41") HSP 1 Score: 88.1965 bits (217), Expect = 1.587e-17 Identity = 69/285 (24.21%), Postives = 123/285 (43.16%), Query Frame = 0
Query: 1811 PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPM------PSVTIRPAG-DSHLPSANTCISRLYVPLYSSKAILKSKLLMAI-KTKNFGF 2087
P+ ++ F+ +GR + + L + F K +L + + D+ DP Y SL + KD + + LE+ F +D + EL P G +++V N Y+ ++RM+ E+ ++ G +V+P L + L+L G+ I+V ++ + + + + +W W V M ++ L+ F G+ +P GF + + G DS LP ++TC +RL +P Y S L KL AI +T+ FG
Sbjct: 604 PDHLHYFKFIGRFIAMALYHGKFIYSGFTMPFYKRMLNKKLIMKDIESIDPEYYNSLMWI------KDNNIEE-CGLEMYFCVDFEVLGEIKPHELKPGGSELKVTEENKEEYIEIVCEWRMSRGIEEQTKSFLEGFNEVVPLEWLQYFDERELELMLCGMQVIDVDDWQRHSIYRHYTKNSKQ-----IQWFWQFVRSMDHEKRSRLLQFVCGTCRVPVG--GFAELMGSNGAQRFCVEKVGKDSWLPRSHTCFNRLDLPPYKSYDQLVEKLNFAIEETEGFGL 874
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Match: EMLSAG00000009470 (supercontig:LSalAtl2s:LSalAtl2s60:50964:63500:-1 gene:EMLSAG00000009470 transcript:EMLSAT00000009470 description:"maker-LSalAtl2s60-augustus-gene-0.20") HSP 1 Score: 79.7221 bits (195), Expect = 8.968e-15 Identity = 77/310 (24.84%), Postives = 131/310 (42.26%), Query Frame = 0
Query: 1815 NAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGR--SIRFHDLAFFDPVIYESLRQLVVDAENK-----------DTSAQLFSALELT------FSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINV-----QTLISYTS----FNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPM-PSVTIRPAG--------DSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGF 2087
N F +G+ + +L N + + F++ ++LL ++ DL DP I ++ L A K D + +L + +D ++ G + +L G+DI VN N++ YV S + + +E ++ G V P +L E+ + G G N +TL+ T FN ES ++L ++E + E++ + F TG P LP GF+ + P +TI D +LPS TC++ L +P YSSK I++ KL +A + F
Sbjct: 1442 NKFTFLGKFIAKAVLDNRMIDMPFSQPFYQWLLREESTLGISDLRNVDPTIASTISSLEGIARKKRKLEQDPQISPDEKLEQIKSLTMDGCPIEDLGLDFTLP-GYPNIDLRKGGKDIPVNIDNLHQYVGLVSHWLLIEGISFQMEALRDGFESVFPISTLQMFYPEELEQIFCGTGQGNFNKWDYKTLVESTKPDHGFNSESPAL--------KYLLEVLESYNIDEQRQFLQFVTGCPRLPVG--GFKALNPPLTIVKKSFDNADADPDDYLPSVMTCVNYLKLPDYSSKKIMREKLRIAAMEGQYCF 1740
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Match: EMLSAG00000003493 (supercontig:LSalAtl2s:LSalAtl2s190:418567:426512:1 gene:EMLSAG00000003493 transcript:EMLSAT00000003493 description:"maker-LSalAtl2s190-snap-gene-4.16") HSP 1 Score: 71.633 bits (174), Expect = 1.831e-12 Identity = 80/296 (27.03%), Postives = 137/296 (46.28%), Query Frame = 0
Query: 1795 TPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRS---IRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAE-LVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGV-GDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPM-PSVTIRPAG-DSH-LPSANTCISRLYVPLYSSKAILKSKLLMAIKT 2082
T +R + +P + + + + +GR++G L +N L + LL +S + L DP +Y +L L +N D Q L L F+I ++ E G S E L NG I V N Y+ + Y++ + GL DV+ L ++ +L+++G +I+V L ++T++ +V F W +VE M+ +K+ L+ F T P GF+ + P + AG + H LP+A+TC++ L +P + + ILK KLL AI++
Sbjct: 728 TKDQRLYPNPSVHKIYEDFLPHYFFIGRMLGKALYENMLVEFPLASFFLHKLLEKSSVKVDIDHLQSLDPELYRNLLYL----KNYDGCVQ---DLGLDFTI-VNEEVGTTSVEELKRNGSSIPVTNVNRTEYIHLIANYKLNKQIRLQSNAFRQGLSDVINLDWLKMFNYKELQLVISGAETEIDVSDLRNHTNYTGGYAVDHPTVVIF----WNVVESMTETQKRHLLKFVTSCSRPPLL--GFKELDPPFCVHNAGAEPHRLPTASTCMNLLKLPNFEEEKILKDKLLYAIES 1009
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Match: EMLSAG00000007808 (supercontig:LSalAtl2s:LSalAtl2s458:200727:208701:-1 gene:EMLSAG00000007808 transcript:EMLSAT00000007808 description:"maker-LSalAtl2s458-snap-gene-2.45") HSP 1 Score: 69.707 bits (169), Expect = 5.761e-12 Identity = 66/257 (25.68%), Postives = 112/257 (43.58%), Query Frame = 0
Query: 1835 PLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGG--GSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPM-PSVTIRPAGDS-HLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGF 2087
P F+ + L G +I P Y S +V + +D L+L F+ ++ E+G S L P+G+ I+V N + Y+ ++YR++ + L K GL + L + + LL +G I+ L + + + S + E W W ++E +S +K + F TGS LP +GF+ + P TIR + LPS++ C + L + +SS +S + AI GF
Sbjct: 436 PFSFSSSFLYQLKGINIGXRHFKVDYPEFYTSRIDYIVKNKVED--------LDLYFTDEIYDEKGHLIESKNLKPSGKKIKVTNFNKFEYLHLLAKYRLSDRIKGPLTKFKTGLHYYVSETYLANFDESEMDLLFSGFSTIDFNDLKKHHNCQNVSHKIME-------WFWLVLEHLSEEQKLLFLKFITGSSRLPP--NGFKDLCPVFTIRQLNEEGKLPSSHVCANVLNIHPHSSFEAFESSFITAITEGYVGF 675
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Match: EMLSAG00000002237 (supercontig:LSalAtl2s:LSalAtl2s1424:34971:40621:1 gene:EMLSAG00000002237 transcript:EMLSAT00000002237 description:"maker-LSalAtl2s1424-augustus-gene-0.25") HSP 1 Score: 60.8474 bits (146), Expect = 3.623e-9 Identity = 28/70 (40.00%), Postives = 44/70 (62.86%), Query Frame = 0
Query: 1629 LSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEP 1698
L + LGERL+P + + P L KITGMLLE+ ++L+ LL +ESL+ + +EA+ ++ H S+P
Sbjct: 590 LEEQKQMLGERLYPLIMRIYPDLVGKITGMLLEMDNSELIHLLECQESLKSKADEAITVLRLHQAQESQP 659
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Match: EMLSAG00000001668 (supercontig:LSalAtl2s:LSalAtl2s128:639100:655047:-1 gene:EMLSAG00000001668 transcript:EMLSAT00000001668 description:"maker-LSalAtl2s128-augustus-gene-6.24") HSP 1 Score: 59.3066 bits (142), Expect = 1.442e-8 Identity = 26/71 (36.62%), Postives = 37/71 (52.11%), Query Frame = 0
Query: 376 CNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDC--WEKCKCRSL 444
CN C+++ T E + Q + C TC + D CT CA+VCH HD K + +CDC E CR++
Sbjct: 1517 CNKLCTYSQTQKEFVFQHWYNCHTCRMVDGSGVCTICAKVCHADHDVTYSKHG-SFFCDCGAKEDGSCRAM 1586
BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Match: gi|68844926|sp|P51592.3|HYD_DROME (RecName: Full=E3 ubiquitin-protein ligase hyd; AltName: Full=HECT-type E3 ubiquitin transferase hyd; AltName: Full=Protein hyperplastic discs) HSP 1 Score: 1051.2 bits (2717), Expect = 0.000e+0 Identity = 685/1730 (39.60%), Postives = 970/1730 (56.07%), Query Frame = 0
Query: 106 ILEERCDGGRNIFHTAVSMCQPTSNKD--PD-----VQDQSAASMSSSSGLDSMDAMSAAYASKA----------------------------------MNLRDMMRRATRLELPSSVGGSQAGPSASTSAPLAPSVAAAAAAAAAVASGGVAPPGDASPPVIEEPSLPSLPWPPEP---------MEGNSGD--EDSLLGLAGSAASISSHKSVLVKERSPATENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKSTS---------------RKT--PELDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKE-----PNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQ---VGSDKGEARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSNDSVSIEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEVNDDGPVENPDQDDQHSDMDLDLL-AETDSESDDGEPN---DRDAKSGGDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRSR--SKSSRTVNGG--FIYIDPNALRRTTSSGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTD----LAP----AQNPNNPTPGRTRTGFQSRAGSLASGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMR--SGTDAQEDSPFGM--DPTPMVPSAASNFDENENDDEAAGSGTPATNMEVDDEDTNQSAAILAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPM----GGEDSGPASMTNTLAVL---PTRLGLSARTTDEAGATSAVQPAEAFNAMSTAPFQATPITGDSNFENVVRQARPSSPTAATCDTASVRSLDTTVTGGNSEEAETDQPQDLSMGGGGTMSSASSVSEEPVASTSKGSFTSPKKAFMMREAARGASSAPEVLV------VPTEKPDQGDVSA-NVTIETSAARRVNSLGVSVPHDIL----------------------LGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMH---FGLIQRLR----GSRD-AQIDRRIQNRFYGRRSRDAARALNFEARPFY-----LNGVGGS-NDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELI 1688
++EERCDG RNIFH V MC P+SNKD PD V+ +S +S + + ++ ++AY S NLRDMM R L +S Q+G S P+A + A +PWP E N D ED + + S++ S ++ + + + +RR +AL L+ +C S P+L +L+ +DAQ TPFM ++ CRAY A ++L + + DP+ ++ +M+FP GS D SPLHVIC NDTCSFTWTGA+HINQ+IFEC+TCGLT SLCCCTECARVCHKGHDCKLK+T+PTAYCDCWEKCKC++LI G+ R+ +LCKL++ TDLV + N +GE+ILLFL+QTVGRQ+ EQRQYR S ++ S RKT E+D DMP+HDL+PP+F+R+AL+ LL DWNA+++MIM+G + P G+ N+ F+ +Q G+ LLDKFTH L+V+C+ D LD LL L+R LQ V + EA +V +RFVRSV RVFV+FN+E P+ K+KS + ++ + C +VFQ L I+IEELCE + AL+APVR G+ RPTAPF++S SN S ++LFSV+P+ P N V+ + + +V H +S + + Q +V A + D E ++ N + N +D E ++ + N+DG QDD+ SD D AET+S+SDD + N R ++G LF +D+SG+S+ E+ S+ GE+D+Q D +F F+E+QLERR+T + RS+LAPQ+MQWA+RSR ++SS V G ++IDP ALRR+T + T E TM TTAS+LARAFGI IRQI++L+++L YN L + +L+I E+I +Q +E +K WDW+ VMD TEAQL+FG+ L+ TD L P AQ ++ TP + + + S ++RRDF Y LSLMR+H +EH D+LPVLD+ +++HIAYV DA +YYMR SG ++D+ G + +PM S + DDE A N+E + D SA+ + + + RRHAFF RS+STL LGC P+ F P+ A+PLAD+PHLLQPN++R++LF P+ +SG + + ++ PTRLG S ++++ E T P ++ TGD N N +A S+ + + D + ++ ++ + + M ++ S S S E + +T +P KA + A++A V+V + T D + SA N++ A + H +L L RW+ LDLFGRVF+DDVG+E GS++ EL GFPVKE +FRR MEKLRN + DL + K+ER R+ LI Q FKE N+ RR QPP+ NRVKVTF++EPGEGSGVARSFYT+++EALL+S K+PNLE Q G++ S + F I R R SRD + + RR N RS +ALN +ARP+ N S NDHLS H Q+GERL+P++ ++ A KITGMLLE+ QLL +++S+E+LRQ+V EA+E+I
Sbjct: 940 VVEERCDGARNIFHACVIMCAPSSNKDSPPDSPSGGVEKKSLVGLSVARSIPTVS--TSAYVSSIAFGASASSSNENSSFATMSSSAAGSASSTSRDNRTNLRDMMNR-----LINSDQAEQSG-----SQPMATNNEDHAY----------------------------IPWPAETPAASNLSASSSQNVSDSIEDDISKIIPSSSQSSMLSNIKLGSPTYTFDLAQRREHALTILQQMCVSPALRPYLCHMLSTKDAQGQTPFMLSVSCRAYEAGIILLNT---ILMLSEQDPQLKE----AMIFPNGSPADQSPLHVICYNDTCSFTWTGADHINQNIFECKTCGLTGSLCCCTECARVCHKGHDCKLKRTAPTAYCDCWEKCKCKALIAGNLTKRFALLCKLVSCTDLVTKFNSKGESILLFLIQTVGRQIVEQRQYRFSVRVRNVSTAATGATGNNSVISNRKTSAAEIDNDMPDHDLEPPKFARKALERLLIDWNAVRSMIMSGAERGDVPNPAGSASENSNSEGFNMFIQTQHGSTLLDKFTHSLIVKCTSDHLDTLLLTLVRELQNASVSNRSKEAEEVVRRFVRSVARVFVIFNLEKQPNPEKRKSHSSCNKYVQSCVKVFQTLHKISIEELCEVSEALIAPVRLGVVRPTAPFTMSSSNLDNS-DDLFSVDPLAPSN---VESPSEQILV---HDAGNDQSANFNIQQNYDVVAMETIRDASE-SEEVINREANSHNQDDE-LIENQRNEDGM-----QDDE-SDNDFTFNDAETESDSDDNQSNQEVQRSVQAGATVGSENDIGVLFLEDESGDSSAQEEDGSEDGESDDQSD-EFNFNEQQLERRST--NSNARSDLAPQTMQWAIRSRDTARSSVRVPTGSNMVFIDPMALRRSTVPASTTVTTPSIEPHTMATTASNLARAFGITIRQISELISILS-YNVLN-DIETSLKIQNDEAIAVQAFVEKRLKATWDWMFTVMDGTEAQLKFGAYLTNYTDPNHPLHPLNLSAQASSSQTPAPATSSSVNGVNIMGS-----NSRRDFFTYCLSLMRSHTSEHRDALPVLDITALRHIAYVLDAFVYYMRNDSGFYDKQDTISGRINNLSPMTESY-------DTDDELA-------NLEEFNADVQMSASSMPSGSQGTRRHAFFARSESTLSLGCSAPEGFELPLDMAMPLADKPHLLQPNSKRQELFA--NLPLLVTTNANNSGATNDGDGGSIFDYTPTRLGFS----------NSLKRNE--RVYETVPIDSSK-TGDGNVTN---KAEGSTDSNIYVQLKKKQGSDDFKSHKEADGNQSKYEKVVLMETDDSLPSTSK-STEALMATRPEVIIAPNKA-----SVSPATAARSVIVLAGGSCLKTIDSDINNYSASNLSTAEQAKCDTQYQKSTSDHLLLFPARGSQFYQSNFSELPSWNFLLSRWKLTLDLFGRVFMDDVGMEHGSVLPELRGFPVKEMRFRRHMEKLRNGQQRDLVLCKLERNRESLIVQTFKELNTQFGNQSRR---IQPPITFNRVKVTFKDEPGEGSGVARSFYTSIAEALLASAKIPNLESVQVGTNHSKYVVPFSSILRSRTVSGSSRDQSTLQRRGSNSKILWRSARERKALNLDARPYTPPNSSDNATPESLNDHLSVHLQQIGERLYPKIHSINQTHAPKITGMLLEIPTPQLLSVISSDETLRQKVNEAIEII 2556 HSP 2 Score: 465.692 bits (1197), Expect = 1.445e-131 Identity = 205/300 (68.33%), Postives = 254/300 (84.67%), Query Frame = 0
Query: 1789 NAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
N PLFY+PGKRGFY+P QG + ER+NAFRN+GRL+GLCLLQNEL PLF RHV+KY+LGR I+FHDLAFFDP +YES RQ++ +A+ K+ + + +EL F IDL EEG G+ EL+P GRD+ V ++N++ YVR+Y++YR+ QEKALE +K G+FDVLP S+ +LTAED RLLLNGVGDINV TLISYT+FNDES E P++L+ FK+W W IVEKM+ +E+QDLVYFWTGSPALPASE+GFQP+PSVTIRPA DSHLP+ANTCISRLY+PLYSSK+IL+SK+LMAIK+KNFGFV
Sbjct: 2587 NEPLFYSPGKRGFYTPRQGFASFERINAFRNIGRLIGLCLLQNELLPLFLQRHVLKYILGRKIKFHDLAFFDPALYESFRQIIQNAQTKE-GEETINRMELCFVIDLMKEEGCGNRELIPGGRDVAVTSSNIFEYVRRYTEYRLIKSQEKALEALKDGVFDVLPDNSMINLTAEDLRLLLNGVGDINVSTLISYTTFNDESSEGPDKLLKFKKWFWSIVEKMNIMERQDLVYFWTGSPALPASEEGFQPLPSVTIRPADDSHLPTANTCISRLYIPLYSSKSILRSKMLMAIKSKNFGFV 2885
BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Match: gi|76363510|sp|Q80TP3.2|UBR5_MOUSE (RecName: Full=E3 ubiquitin-protein ligase UBR5; AltName: Full=E3 ubiquitin-protein ligase, HECT domain-containing 1; AltName: Full=HECT-type E3 ubiquitin transferase UBR5; AltName: Full=Hyperplastic discs protein homolog) HSP 1 Score: 790.415 bits (2040), Expect = 0.000e+0 Identity = 526/1303 (40.37%), Postives = 737/1303 (56.56%), Query Frame = 0
Query: 68 VSRLPMLSPEVRC--VGIKRTLAELESDPTSPNTIETVQRILEERCDGGRNIFHTAVSMCQPTSNKDPDVQDQSAASMSSSSG--LDSMDAMSAAYA---------------SKAMNLRDMMRRATRLELPSSVGGSQAGPSASTSA-PLAPSVAAAAAAAAAVASGGVAPPGDASPPVIEEPSLPSLPWPPEPMEGN-SGDEDSLL----GLAGSAASISSHKSVLVKERSPATE-----NQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKSTSRKT--PELDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTG---HKEPNGTGGRNTPMY-EDQAFLASQSGTGLLDKFTHCLLVRCSGD--SLDCLLTALIRRLQVGSDKG---EARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSL-SPSNDSVS-IEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNED----------ENQIGEDAEDGLDGEVNDDGPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG----------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRS------------RSKSSRTVNGGFIYIDPNALRRTTS----SGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPG-LARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTPG------------------RTRTGFQSRAGSLASGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRS-GTDAQEDSPF--GMDPTPMVPSAASNFD-ENENDDEAAGSGTPATNMEVDDEDTNQSAAILAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMT-------NTLAVLPTRLGLSA 1260
V + ++ +RC ++ L LE + +Q + RCDG RNI H VS+C PTSNK+ ++++ S ++ L +++A++ A + S+++ LR+MMRR+ R + +G +AG S+S P++P +A PP W P+P + GD D +L G +AA+ S P+TE +++R++NA LKL+C+S V P+L LL+ +DA+ TPFM+A+ RAY AA+ + E AQK+A + E ++ M MV P G+ PD+SPL+V+CCNDTCSFTWTGAEHINQDIFECRTCGL +SLCCCTECARVCHKGHDCKLK+TSPTAYCDCWEKCKC++LI G + AR ++L +L+ T+LV N RGE++LLFLVQTV RQ E QYRP R R+ +RKT PE D DMP+HDL+PPRF++ AL+ +L+DWNAL++MIM G +K+P R + E+Q +L QSGT LD FTHCL+V+C+ D LD LL L++ LQ G EA V RF+RSV RVFV+ ++EMA S KK I QP+ +C+RVFQAL+ A+EELC A +L+ PVR GI+RPTAPF+L S S D++ EELFSVEP+ PR S +S+S S ++R N Q +S S + E D + D + E GE N + + + D+ SDM+LDLLA ++ESD + +++D SG G A+ A FS+DDS + + S + D + + TF ++ R T + + AP+SMQWA+R+ + + + G IYIDP+ LRR+ + + A A S + T+ASSLARA+ IVIRQI+DL+ ++ YN L + ++++YQ+++NLQ +E + P W+W+++VMDSTEAQLR+GSAL+ + D +PN+P RT G + RA L++ Q R DFLNYALSLMR+HN EHSD LPVLDV S+KH+AYVF ALIY++++ D+P ++ N D E+ENDD+ + S T ++D+D + + A G+ H FF+RSDS LGC PP+PF P+ EA+PLADQPHLLQPNAR+EDLFG P Q + +G N L VLPT++ +A
Sbjct: 865 VEKQTLMQHILRCDYEACRQYLVNLEQAVVLEQNRQMLQTFISHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSNSRSLRLREMMRRSLR---AAGLGRHEAGASSSDHQDPVSPPIA---------------PPS----------------WVPDPPSMDPDGDIDFILAPAVGSLTTAATGSGQGPSTSTIPGPSTEPSVVESKDRKANAHFILKLLCDSAVLQPYLRELLSAKDARGMTPFMSAVSGRAYSAAITILETAQKIAKAEVSASEKEEDVFMGMVCPSGTNPDDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTATNLVTLPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKTASPE-DSDMPDHDLEPPRFAQLALERVLQDWNALRSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGTIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAIAVTMRFLRSVARVFVILSVEMA---SSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQASSSSQSQSSY-----IIR-----NPQQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENAEAEGHHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDIEQE---TFMLDEPLERTTNSSHANGAAQAPRSMQWAVRNPQHQRAASTAPSSTSTPAASSAGLIYIDPSNLRRSGTISTSAAAAAAALEASNASSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLTYQDAVNLQNYVEEKLIPTWNWMVSVMDSTEAQLRYGSALASAGDPG---HPNHPLHASQNSARRERMTAREEASLRTLEG-RRRATLLSARQGMMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGIDNEDSEHENDDDTSQSAT------LNDKDDD------SLPAETGQNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSSAGSGKCIVEVTMDRNCLEVLPTKMSYAA 2100 HSP 2 Score: 455.677 bits (1171), Expect = 1.578e-128 Identity = 205/300 (68.33%), Postives = 244/300 (81.33%), Query Frame = 0
Query: 1789 NAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
NAPLFY PGKRGFY+P G+ T R+N FRN+GR++GLCLLQNELCP+ NRHVIK LLGR + +HD AFFDPV+YESLRQL++ +++ D A +FSA++L F+IDL EEGGG EL+PNG +I V NVY YVRKY+++RM E+ L M+ GL DVLP SL+ LTAEDFRLL+NG G++NVQ LIS+TSFNDESGE E+L+ FKRW W IVEKMS E+QDLVYFWT SP+LPASE+GFQPMPS+TIRP D HLP+ANTCISRLYVPLYSSK ILK KLL+AIKTKNFGFV
Sbjct: 2494 NAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADA-VFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV 2792 HSP 3 Score: 260.766 bits (665), Expect = 2.562e-68 Identity = 153/285 (53.68%), Postives = 207/285 (72.63%), Query Frame = 0
Query: 1421 LGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFY---GRRS-------RDAARALNFEARPFYLNGVGGSNDH---LSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHG 1692
+G+ + HD+LLGRWR +L+LFGRVF++DVG EPGSI++ELGGF VKE+KFRREMEKLRN ++ DL++ +++R+RD LI Q ++ N+H RR +++ P+ V+RVKVTF++EPGEGSGVARSFYTA+++A LS+ KLPNL+ Q+ ++K H L+QRLR + +R + G R+ RD R L+ + RPF G +D L H+ LGERL+PRVQA++P A+KITGMLLELSPAQLLLLLASE+SLR RV+EA+ELI +HG
Sbjct: 2170 MGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLSL-EVDRDRDLLIQQTMRQLNNHFG---RRCATT--PMAVHRVKVTFKDEPGEGSGVARSFYTAIAQAFLSNEKLPNLDCIQN-ANKGTHTSLMQRLRNRGERDREREREREMRRSSGLRAGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHG 2447
BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Match: gi|20137621|sp|O95071.2|UBR5_HUMAN (RecName: Full=E3 ubiquitin-protein ligase UBR5; AltName: Full=E3 ubiquitin-protein ligase, HECT domain-containing 1; AltName: Full=HECT-type E3 ubiquitin transferase UBR5; AltName: Full=Hyperplastic discs protein homolog; Short=hHYD; AltName: Full=Progestin-induced protein) HSP 1 Score: 787.334 bits (2032), Expect = 0.000e+0 Identity = 528/1303 (40.52%), Postives = 739/1303 (56.72%), Query Frame = 0
Query: 68 VSRLPMLSPEVRC--VGIKRTLAELESDPTSPNTIETVQRILEERCDGGRNIFHTAVSMCQPTSNKDPDVQDQSAASMSSSSG--LDSMDAMSAAYASKAMN---------------LRDMMRRATRLELPSSVGGSQAGPSASTSA-PLAPSVAAAAAAAAAVASGGVAPPGDASPPVIEEPSLPSLPWPPEPMEGN-SGDEDSLL----GLAGSAASISSHKSVLVKERSPATE-----NQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKSTSRKT--PELDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTG---HKEPNGTGGRNTPMY-EDQAFLASQSGTGLLDKFTHCLLVRCSGD--SLDCLLTALIRRLQVGSDKG---EARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSL-SPSNDSVS-IEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNED----------ENQIGEDAEDGLDGEVNDDGPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG----------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRS------------RSKSSRTVNGGFIYIDPNALRRTTS----SGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPG-LARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTPG------------------RTRTGFQSRAGSLASGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRS-GTDAQEDSPF--GMDPTPMVPSAASNFD-ENENDDEAAGSGTPATNMEVDDEDTNQSAAILAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPM--GGEDSGPASMT-----NTLAVLPTRLGLSA 1260
V + ++ +RC ++ L LE ++ +Q + RCDG RNI H VS+C PTSNK+ ++++ S ++ L +++A++ A + + N LR+MMRR+ R + +G +AG S+S P++P +A PP W P+P + GD D +L G +AA+ + P+TE +++R++NA LKL+C+S+V P+L LL+ +DA+ TPFM+A+ RAYPAA+ + E AQK+A + E ++ M MV P G+ PD+SPL+V+CCNDTCSFTWTGAEHINQDIFECRTCGL +SLCCCTECARVCHKGHDCKLK+TSPTAYCDCWEKCKC++LI G + AR ++L +L+ T+LV N RGE++LLFLVQTV RQ E QYRP R R+ +RKT PE D DMP+HDL+PPRF++ AL+ +L+DWNALK+MIM G +K+P R + E+Q +L QSGT LD FTHCL+V+C+ D LD LL L++ LQ G EA V RF+RSV RVFV+ ++EMA S KK I QP+ +C+RVFQAL+ A+EELC A +L+ PVR GI+RPTAPF+L S S D++ EELFSVEP+ PR S +S+S S ++R N Q +S S + E D + D + E GE N + ++ + D+ SDM+LDLLA ++ESD + +++D SG G A+ A FS+DDS + + S + D + + TF ++ R T + + AP+SMQWA+R+ + + + G IYIDP+ LRR+ + + A A S + T+ASSLARA+ IVIRQI+DL+ ++ YN L + ++++YQ+++NLQ +E + P W+W++++MDSTEAQLR+GSAL+ + D +PN+P RT G + RA L++ Q R DFLNYALSLMR+HN EHSD LPVLDV S+KH+AYVF ALIY++++ D+P ++ N D E+ENDD+ S T ++D+D + + A G+ H FF+RSDS LGC PP+PF P+ EA+PLADQPHLLQPNAR+EDLFG P Q + SG M N L VLPT++ +A
Sbjct: 871 VEKQTLMQHILRCDYEACRQYLMNLEQAVVLEQNLQMLQTFISHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLR---AAGLGRHEAGASSSDHQDPVSPPIA---------------PPS----------------WVPDPPAMDPDGDIDFILAPAVGSLTTAATGTGQGPSTSTIPGPSTEPSVVESKDRKANAHFILKLLCDSVVLQPYLRELLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEISSSEKEEDVFMGMVCPSGTNPDDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTATNLVTLPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKTASPE-DSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGTIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAIAVTMRFLRSVARVFVILSVEMA---SSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSSSSSQSQSSY-----IIR-----NPQQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENAEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDIEQE---TFMLDEPLERTTNSSHANGAAQAPRSMQWAVRNTQHQRAASTAPSSTSTPAASSAGLIYIDPSNLRRSGTISTSAAAAAAALEASNASSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLTYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALASAGDPG---HPNHPLHASQNSARRERMTAREEASLRTLEG-RRRATLLSARQGMMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGIDNEDSEHENDDDTNQSAT------LNDKDDD------SLPAETGQNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSSASSGKCLMEVTVDRNCLEVLPTKMSYAA 2106 HSP 2 Score: 455.677 bits (1171), Expect = 1.701e-128 Identity = 205/300 (68.33%), Postives = 244/300 (81.33%), Query Frame = 0
Query: 1789 NAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
NAPLFY PGKRGFY+P G+ T R+N FRN+GR++GLCLLQNELCP+ NRHVIK LLGR + +HD AFFDPV+YESLRQL++ +++ D A +FSA++L F+IDL EEGGG EL+PNG +I V NVY YVRKY+++RM E+ L M+ GL DVLP SL+ LTAEDFRLL+NG G++NVQ LIS+TSFNDESGE E+L+ FKRW W IVEKMS E+QDLVYFWT SP+LPASE+GFQPMPS+TIRP D HLP+ANTCISRLYVPLYSSK ILK KLL+AIKTKNFGFV
Sbjct: 2501 NAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADA-VFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV 2799 HSP 3 Score: 261.922 bits (668), Expect = 1.066e-68 Identity = 154/285 (54.04%), Postives = 207/285 (72.63%), Query Frame = 0
Query: 1421 LGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFY---GRRS-------RDAARALNFEARPFYLNGVGGSNDH---LSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHG 1692
+G+ + HD+LLGRWR +L+LFGRVF++DVG EPGSI++ELGGF VKE+KFRREMEKLRN ++ DL++ +++R+RD LI Q ++ N+H RR +++ P+ V+RVKVTF++EPGEGSGVARSFYTA+++A LS+ KLPNLE Q+ ++K H L+QRLR + +R + G R+ RD R L+ + RPF G +D L H+ LGERL+PRVQA++P A+KITGMLLELSPAQLLLLLASE+SLR RV+EA+ELI +HG
Sbjct: 2176 MGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLSL-EVDRDRDLLIQQTMRQLNNHFG---RRCATT--PMAVHRVKVTFKDEPGEGSGVARSFYTAIAQAFLSNEKLPNLECIQN-ANKGTHTSLMQRLRNRGERDREREREREMRRSSGLRAGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPEPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHG 2453
BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Match: gi|378405229|sp|Q62671.3|UBR5_RAT (RecName: Full=E3 ubiquitin-protein ligase UBR5; AltName: Full=100 kDa protein; AltName: Full=E3 ubiquitin-protein ligase, HECT domain-containing 1; AltName: Full=HECT-type E3 ubiquitin transferase UBR5; AltName: Full=Hyperplastic discs protein homolog) HSP 1 Score: 787.334 bits (2032), Expect = 0.000e+0 Identity = 526/1303 (40.37%), Postives = 735/1303 (56.41%), Query Frame = 0
Query: 68 VSRLPMLSPEVRC--VGIKRTLAELESDPTSPNTIETVQRILEERCDGGRNIFHTAVSMCQPTSNKDPDVQDQSAASMSSSSG--LDSMDAMSAAYASKAMN---------------LRDMMRRATRLELPSSVGGSQAGPSASTSA-PLAPSVAAAAAAAAAVASGGVAPPGDASPPVIEEPSLPSLPWPPEPMEGN-SGDEDSLL----GLAGSAASISSHKSVLVKERSPATE-----NQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKSTSRKT--PELDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTG---HKEPNGTGGRNTPMY-EDQAFLASQSGTGLLDKFTHCLLVRCSGD--SLDCLLTALIRRLQVGSDKG---EARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSL-SPSNDSVS-IEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNED----------ENQIGEDAEDGLDGEVNDDGPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG----------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRS------------RSKSSRTVNGGFIYIDPNALRRTTS----SGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPG-LARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTPG------------------RTRTGFQSRAGSLASGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRS-GTDAQEDSPF--GMDPTPMVPSAASNFD-ENENDDEAAGSGTPATNMEVDDEDTNQSAAILAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMT-------NTLAVLPTRLGLSA 1260
V + ++ +RC ++ L LE ++ +Q + RCDG RNI H VS+C PTSNK+ ++++ S ++ L +++A++ A + + N LR+MMRR+ R + +G +AG S+S P++P +A PP W P+P + GD D +L G +AA+ P+TE +++R++NA LKL+C+S V P+L LL+ +DA+ TPFM+A+ RAYPAA+ + E AQK+A + E ++ M MV P G+ PD+SPL+V+CCNDTCSFTWTGAEHINQDIFECRTCGL +SLCCCTECARVCHKGHDCKLK+TSPTAYCDCWEKCKC++LI G + AR ++L +L+ T+LV N RGE++LLFLVQTV RQ E QYRP R R+ +RKT P+ D DMP+HDL+PPRF++ AL+ +L+DWNAL++MIM G +K+P R + E+Q +L QSGT LD FTHCL+V+C+ D LD LL L++ LQ G EA V RF+RSV RVFV+ ++EMA S KK I QP+ +C+RVFQAL+ A+EELC A +L+ PVR GI+RPTAPF+L S S D++ EELFSVEP+ PR S +S+S S ++R N Q +S S + E D + D + E GE N + + + D+ SDM+LDLLA ++ESD + +++D SG G A+ A FS+DDS + + S + D + + TF ++ R T + + AP+SMQWA+R+ + + + G IYIDP+ LRR+ + + A A S + T+ASSLARA+ IVIRQI+DL+ ++ YN L + ++++YQ+++NLQ +E + P W+W++++MDSTEAQLR+GSAL+ + D +PN+P RT G + RA L++ Q R DFLNYALSLMR+HN EHSD LPVLDV S+KH+AYVF ALIY++++ D+P ++ N D E+ENDD+ + S AT + DDE + A G+ H FF+RSDS LGC PP+PF P+ EA+PLADQPHLLQPNAR+EDLFG P Q + +G N L VLPT++ +A
Sbjct: 860 VEKQTLMQHILRCDYEACRQYLVNLEQAVVLEQNLQMLQTFISHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLR---AAGLGRHEAGASSSDHQDPVSPPIA---------------PPS----------------WVPDPPSMDPDGDIDFILAPAVGSLTTAATGGGQGPSTSTIPGPSTEPSVVESKDRKANAHFILKLLCDSAVLQPYLRELLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEVSGSEKEEDVFMGMVCPSGTNPDDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTATNLVTLPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKTASPD-DSDMPDHDLEPPRFAQLALERVLQDWNALRSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGTIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAIAVTMRFLRSVARVFVILSVEMA---SSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSSSSSQSQSSY-----IIR-----NPQQRRISQSQPVRGREEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENAEAEGHHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDIEQE---TFMLDEPLERTTNSSHANGAAQAPRSMQWAVRNTQHQRAASTAPSSTSTPAASSAGLIYIDPSNLRRSGTISTSAAAAAAALEASNASSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLTYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALASAGDPG---HPNHPLHASQNSARRERMTAREEASLRTLEGRRRRATLLSARQGMMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGIDNEDSEHENDDDTSQS---ATLNDKDDE---------SLPAETGQNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSSAGSGKCLVEVTMDRNCLEVLPTKMSYAA 2096 HSP 2 Score: 453.751 bits (1166), Expect = 7.030e-128 Identity = 203/300 (67.67%), Postives = 244/300 (81.33%), Query Frame = 0
Query: 1789 NAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
NAPLFY PGKRGFY+P G+ T R+N FRN+GR++GLCLLQNELCP+ NRHVIK LLGR + +HD AFFDPV+YESLRQL++ +++ D A +FSA++L F++DL EEGGG EL+PNG +I V NVY YVRKY+++RM E+ L M+ GL DVLP SL+ LTAEDFRLL+NG G++NVQ LIS+TSFNDESGE E+L+ FKRW W IVE+MS E+QDLVYFWT SP+LPASE+GFQPMPS+TIRP D HLP+ANTCISRLYVPLYSSK ILK KLL+AIKTKNFGFV
Sbjct: 2490 NAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADA-VFSAMDLAFAVDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVERMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV 2788 HSP 3 Score: 262.307 bits (669), Expect = 9.603e-69 Identity = 154/285 (54.04%), Postives = 207/285 (72.63%), Query Frame = 0
Query: 1421 LGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFY---GRRS-------RDAARALNFEARPFYLNGVGGSNDH---LSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHG 1692
+G+ + HD+LLGRWR +L+LFGRVF++DVG EPGSI++ELGGF VKE+KFRREMEKLRN ++ DL++ +++R+RD LI Q ++ N+H RR +++ P+ V+RVKVTF++EPGEGSGVARSFYTA+++A LS+ KLPNL+ Q+ ++K H L+QRLR + +R + G R+ RD R L+ + RPF G +D L H+ LGERL+PRVQA++P A+KITGMLLELSPAQLLLLLASE+SLR RVEEA+ELI +HG
Sbjct: 2166 MGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLSL-EVDRDRDLLIQQTMRQLNNHFG---RRCATT--PMAVHRVKVTFKDEPGEGSGVARSFYTAIAQAFLSNEKLPNLDCIQN-ANKGTHTSLMQRLRNRGERDREREREREMRRSSGLRAGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPDPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVEEAMELIVAHG 2443
BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Match: gi|46397059|sp|Q9UTG2.1|PUB2_SCHPO (RecName: Full=E3 ubiquitin-protein ligase pub2; AltName: Full=HECT-type E3 ubiquitin transferase pub2) HSP 1 Score: 127.872 bits (320), Expect = 1.368e-28 Identity = 90/284 (31.69%), Postives = 146/284 (51.41%), Query Frame = 0
Query: 1811 PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMP------SVTIRPAGD-SHLPSANTCISRLYVPLYSSKAILKSKLLMAIK-TKNFG 2086
P+ + FR VGR++GL + + F K +L + + D+ D V YESL+ + +N D +L L FS++ + + +L+PNGR+I VN N NY++ +++++ + E+ +K GL +++P L + LLNG DI+VQ +T + + ET + ++ W W ++ + SP +K L+ F TG+ LP S GF+ M TI G S LP A+TC +RL +P Y+SK L+ KL +AI+ T FG
Sbjct: 401 PDFRSYFRFVGRVMGLAIYHRRYLDVQFVLPFYKRILQKPLCLEDVKDVDEVYYESLKWI----KNNDVD----ESLCLNFSVEENRFGESVTVDLIPNGRNIAVNNQNKMNYLKALTEHKLVTSTEEQFNALKGGLNELIPDSVLQIFNENELDTLLNGKRDIDVQDWKRFTDYRSYT-ETDDIVI----WFWELLSEWSPEKKAKLLQFATGTSRLPLS--GFKDMHGSDGPRKFTIEKVGHISQLPKAHTCFNRLDIPPYNSKEELEQKLTIAIQETAGFG 669
BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Match: gi|378548424|sp|F1N6G5.3|HACE1_BOVIN (RecName: Full=E3 ubiquitin-protein ligase HACE1; AltName: Full=HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase HACE1) HSP 1 Score: 122.865 bits (307), Expect = 1.240e-26 Identity = 86/283 (30.39%), Postives = 142/283 (50.18%), Query Frame = 0
Query: 1811 PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALP----ASEDGFQPMPSVTIR--PAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGF 2087
P+ +N FR G+++GL L +L ++F R K++LG + + D+A DP ++L Q ++D + D LELTFS++ + L P G I V N YV+ ++ RM + + G +P + + LLL+G+ +I+V I T + SG E V +W W +VE ++P E+ L+ F TGS +P A+ G + + TI P + LP+++TCI+ L +P Y SK ILK +LL+A+ ++G+
Sbjct: 635 PDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNL-QWILDNDISDL------GLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYT--SGYEREDPV--IQWFWEVVEDITPEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGY 906
BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Match: gi|378548354|sp|D3ZBM7.1|HACE1_RAT (RecName: Full=E3 ubiquitin-protein ligase HACE1; AltName: Full=HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase HACE1) HSP 1 Score: 120.939 bits (302), Expect = 3.927e-26 Identity = 86/283 (30.39%), Postives = 141/283 (49.82%), Query Frame = 0
Query: 1811 PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALP----ASEDGFQPMPSVTIR--PAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGF 2087
P+ +N FR G+++GL L +L ++F R K++LG + + D+A DP ++L Q ++D + D LELTFS++ + L P G I V N YV+ ++ RM + + G +P + + LLL+G+ +I+V I T + SG E V +W W +VE M+ E+ L+ F TGS +P A+ G + + TI P + LP+++TCI+ L +P Y SK ILK +LL+A+ ++G+
Sbjct: 635 PDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNL-QWILDNDISDL------GLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWIKNTEYT--SGYEREDPV--IQWFWEVVEDMTQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGY 906
BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Match: gi|378548416|sp|E1C656.1|HACE1_CHICK (RecName: Full=E3 ubiquitin-protein ligase HACE1; AltName: Full=HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase HACE1) HSP 1 Score: 120.168 bits (300), Expect = 7.436e-26 Identity = 83/283 (29.33%), Postives = 139/283 (49.12%), Query Frame = 0
Query: 1811 PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALP----ASEDGFQPMPSVTIR--PAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGF 2087
P+ +N FR G+++GL L +L ++F R K++LG + + D+A DP ++L Q ++D + D LELTFS++ + L P G I V N YV+ ++ RM + + G +P + + LLL+G+ +I+V + N E ER +W W +VE+++ E+ L+ F TGS +P A G + + TI P + LP+++TCI+ L +P Y SK ILK +LL+A+ ++G+
Sbjct: 668 PDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNL-QWILDNDISDL------GLELTFSVETDVFGAMEEVPLKPGGASILVTQENKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWLK----NTEYTSGYERGDQVIQWFWDVVEELTQEERVLLLQFVTGSSRVPHGGFAHIMGGSGLQNFTIAAVPYTANLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGY 939
BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Match: gi|123796888|sp|Q3U0D9.1|HACE1_MOUSE (RecName: Full=E3 ubiquitin-protein ligase HACE1; AltName: Full=HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase HACE1) HSP 1 Score: 119.783 bits (299), Expect = 9.876e-26 Identity = 85/283 (30.04%), Postives = 141/283 (49.82%), Query Frame = 0
Query: 1811 PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALP----ASEDGFQPMPSVTIR--PAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGF 2087
P+ +N FR G+++GL L +L ++F R K++LG + + D+A DP ++L Q ++D + D LELTFS++ + L P G I V N YV+ ++ RM + + G +P + + LLL+G+ +I+V I T + SG E V +W W +VE ++ E+ L+ F TGS +P A+ G + + TI P + LP+++TCI+ L +P Y SK ILK +LL+A+ ++G+
Sbjct: 635 PDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNL-QWILDNDISDL------GLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWIKNTEYT--SGYEREDPV--IQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGY 906
BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Match: gi|134034136|sp|Q8IYU2.2|HACE1_HUMAN (RecName: Full=E3 ubiquitin-protein ligase HACE1; AltName: Full=HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1; AltName: Full=HECT-type E3 ubiquitin transferase HACE1) HSP 1 Score: 119.398 bits (298), Expect = 1.320e-25 Identity = 85/283 (30.04%), Postives = 141/283 (49.82%), Query Frame = 0
Query: 1811 PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALP----ASEDGFQPMPSVTIR--PAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGF 2087
P+ +N FR G+++GL L +L ++F R K++LG + + D+A DP ++L Q ++D + D LELTFS++ + L P G I V N YV+ ++ RM + + G +P + + LLL+G+ +I+V I T + SG E V +W W +VE ++ E+ L+ F TGS +P A+ G + + TI P + LP+++TCI+ L +P Y SK ILK +LL+A+ ++G+
Sbjct: 635 PDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNL-QWILDNDISDL------GLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVSDWIKNTEYT--SGYEREDPV--IQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGY 906
BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Match: gi|972203203|ref|XP_015183734.1| (PREDICTED: E3 ubiquitin-protein ligase hyd isoform X2 [Polistes dominula]) HSP 1 Score: 1816.59 bits (4704), Expect = 0.000e+0 Identity = 1017/1995 (50.98%), Postives = 1294/1995 (64.86%), Query Frame = 0
Query: 216 VAPPGDASPPVIEEPSLPSLPWPPEPMEGNSGDEDSLLGLAGSAASISSHKSV-LVKERSPATENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKST--SRKTPE-----LDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQVGSDKG---EARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSNDSV--SIEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEV---------NDDGPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG---------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRSRSKSSRT----VNGG--FIYIDPNALRRTTSSGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTP---GRTRTGFQSRAG-SLA----------SGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMR-------SGTDAQEDSPFGMDPTPMVPSAASNFDENENDDEAAGSGTPATN-MEVDDED------TNQSAAILAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMTNTLAVLPTRLGLSARTTDEAGATSAVQPAEAFNAMSTAPFQATPITGDSNFENVVRQARPSSPTAATCDTASVRSLDTTVTGGNSEEAET--DQPQDLSMG-------------GGGTMSSASSVSEEPVASTSKGSFTSPKKAFMMREAARGAS-----SAPEVLVVP-------TEKPDQG----DVSANVTIETS---AARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFYGRRSRDAARALNFEARPFY----------------------LNGVGGSNDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGSSGSSAAAAGVAGQEHLLPDLDVFNLGNSSGVKRKSLSGSVALGSEPELQEEENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
V P + S P IEEP +P+L WPPE + SG+EDSLL + ++ S+ + ++ + ++ ER++NA+ LK +CE+ V APHL+ LL +DAQ TP M A+ RAY AAL+L + QKV D K +M+ PP + PD SPL V CCNDTCSFTWTGA+HINQDIFECRTCGLT SLCCCTECARVCH+GHDCKLK+TSPTAYCDCWEKCKCR+LI G+Q ARY++LC+L+ +TDL + N RGE ILLFL QTVGRQ EQRQ+RP+ +ST SRK P D DMP+HDL+PPRFSR AL+ LL DW+A++ MIM+G E NG N ++ DQ L QSGT LLDKFTH LLV+CS + LD LL LIR L + G EA VA+RFVRSV R+FV+F IEMAP+ +K+KS SQPL +CR+VFQALI +AIEELCETA++L+APVR G++RPTAPF+L+ S V EELFS+EP++P + +S Q + + V+ + + A +VSA + ++H D + I E E G+ G V ++ G V + SD +LDLLAE ++ESD D +++DA S ++ G LF +D+SGES+ ED ES+AGETDEQD+ DF +EQLERR+ R+NLAP SMQWA+R+R S+RT V GG ++IDP +LRRTT++ A E +TM TTAS LARAFGIVIRQIADLL M+QDY +LAP L + +EI+ Q+++NLQL +E H+KP WDWL+ VMD+TEAQLRFG +L+ S D ++P N P G TG S A SL SG D RR+FL+Y LSLMRAHN EH DSLPVLDV++++H+AYVFDAL+YYMR S + Q++S + + + DEN+N DE +PA N +E D D + + + + + KGR+H FFQRSDSTLCLGCPP DPF T M EALPLADQPHLLQP++RREDLFG+P+QP G + P LA LPTRLGLSAR D N MS+ Q P G + +R+ P+A+ D+ S++ D ++E++ P LS + ++S + S S ++ ++R A+ SAPE+L +P +E+ D ++SA+ T+ TS + R + ++G ++ H+ILLGRWR +LDLFGRVF++DVGLE GSI+SELGGF VKEAKFRR MEKLRNS+ D+T++KIER+R QL+ Q KE N+ N + +R+S+S PPL VNRVKVTF++EPGEGSGVARSFYTA++EALL++ KLP+LE AQ G SK + ++Q+L+ SRD D R QN + R+ +AL+FEAR F+ L +N+HL+ HQ QLG+RL+P+V ALRP LA KITGMLLELSPAQLL+LLASE++LRQ+VEEA ELI SH +++ E LL DLDVF+L +K L ++ ++ EDNAPLFY+P KRGFY+P QGR + ER+NAFRNVGRL+GLCLLQNELCP+F NRHVIKY+L R IRFHDLAFFD VIYESLRQLV+DAE KD+++ LFSAL+LTFSIDL EEGGGS EL+ +GRDIEV A+NVY+YVRKY++ RM QEKAL M+ G+FDVLP G+LD LT+ED RLLLNGVGDINV LISYTSFNDESGE E+LV FKRWLW IVEKMS +E+QDLVYFWTGSPALPASEDGFQPMPSVTIRPA D+HLP+ANTCISRLYVPLYSS+ +L+ KLL+AIKTKNFGFV
Sbjct: 948 VPNPDNTSAP-IEEP-IPTLSWPPEAFDNTSGEEDSLLSIGAASISMMNKPNIGAASNNTYIVDSNERKNNAILILKYMCENNVLAPHLKELLTAKDAQGQTPLMLAVSVRAYHAALILLDTIQKVGRD--------SKECSAMILPPDANPDLSPLFVTCCNDTCSFTWTGADHINQDIFECRTCGLTGSLCCCTECARVCHRGHDCKLKRTSPTAYCDCWEKCKCRALIAGYQVARYDLLCRLVVNTDLATKINSRGECILLFLAQTVGRQFIEQRQFRPATRPRSTSASRKAPSSDGLGADSDMPDHDLEPPRFSRNALERLLNDWSAVQCMIMSGVSE-NG----NEQLFGDQGQLCRQSGTALLDKFTHLLLVKCSSEMLDILLITLIRELHNETIPGRQEEANNVARRFVRSVARIFVIFTIEMAPNTAKRKSASQASQPLMKCRKVFQALIKLAIEELCETADSLIAPVRLGVARPTAPFTLTSSAVEVINGSEELFSIEPLIPHSGVSSQTLDGGLQ---------TQQVNNNITIARDVSAMDEVEGGEDVHMDMDGD----ISEHDEPGVSGTVAGQPLGEVDSNAGGVGEEQAGEGESDTELDLLAEAETESDSDDNHSNQDAASAQRSVQTGATAGSDGGMGSILLFPEDESGESSQQEDDESEAGETDEQDNEDFQNGDEQLERRSGSSGHLHRNNLAPVSMQWAIRNRESSTRTAGLRVTGGSNLVFIDPVSLRRTTATSAVAAA---QEPITMGTTASCLARAFGIVIRQIADLLVMIQDYKTLAPSLPQLMEITLQDALNLQLYLESHLKPTWDWLLTVMDATEAQLRFGVSLTRSADPTHPEHPLNNAPSLSGGNFTGLLSSAALSLTLQGNASRNQRSGGDLHSARREFLSYCLSLMRAHNGEHRDSLPVLDVSALRHVAYVFDALVYYMRCLSEPLTSRGETQKESNYN--------ATWNEQDENDN-DEGEEYNSPAPNALEPDSVDYPDLLQVSMNGSGNSNSTPKGRKHPFFQRSDSTLCLGCPPLDPFDTVMSEALPLADQPHLLQPHSRREDLFGIPRQPTGTANQNP------LAGLPTRLGLSARGADNQ------------NNMSSTFGQIVP--GPTFTSTEIRR-----PSASAGDSVSMKYTDKLKFHRATDESQMPLSSPMPLSSAIPFPVNQLLQQHIDRAPIIVSTSNQNDSGNSKSTKDLCKTNRSVIVRAASSMVDSLHKLSAPEILFIPSVENSDVSEEVDPAVTSQEISAHETVVTSPVESTRTILNIGTNISHNILLGRWRLSLDLFGRVFMEDVGLEAGSIVSELGGFSVKEAKFRRNMEKLRNSQQKDITLSKIERDRTQLLVQTMKELNTQYNLYNKRTSNS-PPLAVNRVKVTFKDEPGEGSGVARSFYTAIAEALLANEKLPSLEAAQVG-SKYTQYNVLQKLK-SRDRDRDLRRQNPRSSGKCRETRKALSFEARSFHPSSSVEGNNASGSGSSSSNTHPLPASHPNNEHLTVHQQQLGDRLYPKVHALRPALAEKITGMLLELSPAQLLMLLASEDALRQKVEEAFELIHSHNQDLT------------------------------------------------SEALL-DLDVFSLTERCAANKKKLETNIM----------DDGEDNAPLFYSPDKRGFYTPRQGRASFERLNAFRNVGRLIGLCLLQNELCPIFLNRHVIKYILSRPIRFHDLAFFDSVIYESLRQLVIDAETKDSNS-LFSALDLTFSIDLCPEEGGGSIELITSGRDIEVTASNVYDYVRKYAEVRMIKVQEKALHTMREGVFDVLPEGALDGLTSEDLRLLLNGVGDINVSVLISYTSFNDESGEATEKLVKFKRWLWSIVEKMSHVERQDLVYFWTGSPALPASEDGFQPMPSVTIRPADDAHLPTANTCISRLYVPLYSSRHVLRHKLLLAIKTKNFGFV 2814
BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Match: gi|954567514|ref|XP_014607787.1| (PREDICTED: E3 ubiquitin-protein ligase hyd isoform X2 [Polistes canadensis]) HSP 1 Score: 1812.35 bits (4693), Expect = 0.000e+0 Identity = 1016/1979 (51.34%), Postives = 1286/1979 (64.98%), Query Frame = 0
Query: 227 IEEPSLPSLPWPPEPMEGNSGDEDSLLGLAGSAASISSHKSV-LVKERSPATENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKST--SRKTPE-----LDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQVGSDKG---EARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSNDSV--SIEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEV---------NDDGPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG---------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRSRSKSSRT----VNGG--FIYIDPNALRRTTSSGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTP---GRTRTGFQSRAG-SLA----------SGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMR-------SGTDAQEDSPFGMDPTPMVPSAASNFDENEND-DEAAGSGTPATNM----EVDDEDTNQSAAILAA---TASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMTNTLAVLPTRLGLSARTTDEAGATSAVQPAEAFNAMSTAP-FQATPITGDSNFENVVRQARPSSPTAATCDTASVRSLDTTVTGGNSEEAETDQPQDLSMGG--------GGTMSSASSVSEEPVASTSKGSFTSPKKAFMMREAARGAS-----SAPEVLVVPT-EKPD----------QGDVSANVTIETS---AARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFYGRRSRDAARALNFEARPFY----------------------LNGVGGSNDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGSSGSSAAAAGVAGQEHLLPDLDVFNLGNSSGVKRKSLSGSVALGSEPELQEEENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
IEEP +P+L WPPE + SG+EDSLL + ++ S+ + S+ + ++ ER++NA+ LK +CE+ V APHL+ LL +DAQ TP M A+ RAY AAL+L + QKV D K +M+ PP + PD SPL V CCNDTCSFTWTGA+HINQDIFECRTCGLT SLCCCTECARVCH+GHDCKLK+TSPTAYCDCWEKCKCR+LI G+Q ARY++LC+L+ +TDL + N RGE ILLFL QTVGRQ EQRQ+RP+ +ST SRK P D DMP+HDL+PPRFSR AL+ LL DW+A++ MIM+G E NG N ++ DQ L QSGT LLDKFTH LLV+CS + LD LL LIR L + G EA VA+RFVRSV R+FV+F IEMAP+ +K+KS SQPL +CR+VFQALI +AIEELCETA++L+APVR G++RPTAPF+L+ S V EELFS+EP++P + +S Q + + V+ + + A +VSA + ++H D + I E E G+ G V ++ G V + SD +LDLLAE ++ESD D +++DA S ++ G LF +D+SGES+ ED ES+AGETDEQD+ DF +EQLERR+ R+NLAP SMQWA+R+R S+RT V GG ++IDP +LRRTT++ A E +TM TTAS LARAFGIVIRQIADLL M+QDY +LAP L + +EI+ Q+++NLQL +E H+KP WDWL+ VMD+TEAQLRFG +L+ S D ++P N P G TG S A SL SG D RR+FL+Y LSLMRAHN EH DSLPVLDV++++H+AYVFDAL+YYMR S +AQ++S + S +++END DE +PA N V+ D Q + + + SKGR+H FFQRSDSTLCLGCPP DPF T M EALPLADQPHLLQP++RREDLFG+P+Q G + P LA LPTRLGLSAR D +S+ F + P F +T I R + +S +A + T V N A P ++ + S S+ SE + +K ++ ++R A+ +APE+L +P+ E PD ++SA+ T+ TS +AR + ++G ++ H+ILLGRWR +LDLFGRVF++DVGLE GSI+SELGGF VKEAKFRR MEKLRNS+ D+T++KIER+R QL+ Q KE N+ N + +R+S+S PPL VNRVKVTF++EPGEGSGVARSFYTA++EALL++ KLP+LE AQ G SK + ++Q+L+ SRD D R QN + R+ +AL+FEAR F+ L +N+HL+ HQ QLG+RL+P+V ALRP LA KITGMLLELSPAQLL+LLASE++LRQ+VEEA ELI SH +++ E LL DLDVF+L +K + ++ ++ EDNAPLFY+P KRGFY+P QGR T ER+NAFRNVGRL+GLCLLQNELCP+F NRHVIKY+L R IRFHDLAFFD VIYESLRQLV+DAE KD+++ LFSAL+LTFSIDL EEGGGS EL+ +GRDIEV A+NVY+YVRKY++ RM QEKAL M+ G+FDVLP G+LD LT+ED RLLLNGVGDINV LISYTSFNDESGE E+LV FKRWLW IVEKMS +E+QDLVYFWTGSPALPASEDGFQPMPSVTIRPA D+HLP+ANTCISRLYVPLYSS+ +L+ KLL+AIKTKNFGFV
Sbjct: 958 IEEP-IPTLSWPPEAFDNTSGEEDSLLSIGAASISMMNKPSIGAAPNNTYIVDSNERKNNAILILKYMCENNVLAPHLKELLTAKDAQGQTPLMLAVSVRAYHAALILLDTIQKVGRD--------SKECSAMILPPDANPDLSPLFVTCCNDTCSFTWTGADHINQDIFECRTCGLTGSLCCCTECARVCHRGHDCKLKRTSPTAYCDCWEKCKCRALIAGYQVARYDLLCRLVVNTDLATKINSRGECILLFLAQTVGRQFIEQRQFRPATRPRSTSASRKAPSSDGLGADSDMPDHDLEPPRFSRNALERLLNDWSAVQCMIMSGVSE-NG----NEQLFGDQGQLCRQSGTALLDKFTHLLLVKCSTEMLDILLITLIRELHNDTIPGRQEEANNVARRFVRSVARIFVIFTIEMAPNTAKRKSASQASQPLMKCRKVFQALIKLAIEELCETADSLIAPVRLGVARPTAPFTLTSSAVEVINGSEELFSIEPLIPHSGVSSQTLDGGLQ---------TQQVNNNITIARDVSAMDEVEGGEDVHMDMDGD----ISEHDEPGVSGTVAGQPLGEVDSNAGGVGEEQAGEGESDTELDLLAEAETESDSDDNHSNQDAASAQRSVQTGATAGSDGGMGSILLFPEDESGESSQQEDDESEAGETDEQDNEDFQNGDEQLERRSGSSGHLHRNNLAPVSMQWAIRNRESSTRTAGLRVTGGSNLVFIDPVSLRRTTATSAVAAA---QEPITMGTTASCLARAFGIVIRQIADLLVMMQDYKTLAPTLPQLMEITLQDALNLQLYLEAHLKPTWDWLLTVMDATEAQLRFGVSLTRSADPTHPEHPLNNAPSLSGGNFTGLLSSAALSLTLQGNASRNQRSGGDLHSARREFLSYCLSLMRAHNGEHRDSLPVLDVSALRHVAYVFDALVYYMRCLSEPLTSRGEAQKES----------ANYTSWHEQDENDNDEGEEYNSPAPNALEPDSVEYPDLLQVSMCGSGNSNSTSKGRKHPFFQRSDSTLCLGCPPLDPFDTVMSEALPLADQPHLLQPHSRREDLFGIPRQQTGTANQNP------LAGLPTRLGLSARGADNQNLSSS-----TFGQIVPGPTFTSTEIR---------RPSASASASAGDSGSIKFSERPTKVFRVNENHAPLTTPLQMTFQNPILEHIDRAPIIVSTSNQSESANSKIAK-DLCKANRSVIVRAASSMVENLHKLTAPEILFIPSVENPDVSEEVDPAVTSQEISAHETVVTSPVESARTILNIGTNISHNILLGRWRLSLDLFGRVFMEDVGLEAGSIVSELGGFSVKEAKFRRNMEKLRNSQQKDITLSKIERDRTQLLVQTMKELNTQYNLYNKRTSNS-PPLAVNRVKVTFKDEPGEGSGVARSFYTAIAEALLANEKLPSLEAAQVG-SKYTQYNVLQKLK-SRDRDRDLRRQNPRSSGKCRETRKALSFEARSFHPSSSVEGNNASGSGSSSSNTHPLPASHPNNEHLTVHQQQLGDRLYPKVHALRPALAEKITGMLLELSPAQLLMLLASEDALRQKVEEAFELIHSHNQDLT------------------------------------------------SEALL-DLDVFSLTERCAANKKKIETNIM----------DDGEDNAPLFYSPDKRGFYTPRQGRATYERLNAFRNVGRLIGLCLLQNELCPIFLNRHVIKYILARPIRFHDLAFFDSVIYESLRQLVIDAETKDSNS-LFSALDLTFSIDLCPEEGGGSIELITSGRDIEVTASNVYDYVRKYAEVRMIKVQEKALHTMREGVFDVLPEGALDGLTSEDLRLLLNGVGDINVSVLISYTSFNDESGEATEKLVKFKRWLWSIVEKMSHVERQDLVYFWTGSPALPASEDGFQPMPSVTIRPADDAHLPTANTCISRLYVPLYSSRHVLRHKLLLAIKTKNFGFV 2812
BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Match: gi|1000730443|ref|XP_015587626.1| (PREDICTED: E3 ubiquitin-protein ligase hyd isoform X5 [Cephus cinctus]) HSP 1 Score: 1806.57 bits (4678), Expect = 0.000e+0 Identity = 1026/1997 (51.38%), Postives = 1281/1997 (64.15%), Query Frame = 0
Query: 220 GDASPPVIEEPSLPSLPWPPEPMEGNSGDEDSLLGLAGSAASISSHKSVLVKERSPAT---ENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYR--PSRSRKSTSRKTPE---------LDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQVGSDKG---EARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSNDSV--SIEELFSVEPMVPRNSLSVQP-ENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEVNDD-GPVENPDQDDQHSDMDLDLLA--ETDSESDDGEPN------DRDAKSGGD--NNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERR-----ATPGTT--QERSNLAPQSMQWALRSRSKSSRTV------NGGFIYIDPNALRRTTSSGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTP---------------------GRTRTGFQSRAGSLASGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRSGT---DAQEDSPFGMDPTPMVPSAASNFDENENDDEAAGSGT---PATNMEVDDEDTNQSAAILAA-------TASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGE-DSGPASMTNTLAVLPTRLGLSARTTDEAGATSAVQPAEAFNAMSTAPFQATPITGDSNFENVVRQ--------ARPSSPTAATCD-----TASVRSLDTTVTGGNSEEAETDQPQDLSMGGGGTMSSASSVSEEPVASTSKGSFTSPKKAFMMREAARGASSAPEVLVVPT-------EKPDQG----DVSANVTIETS---AARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFYGRRSRDAARALNFEARPFY----------------------LNGVGGSNDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGSSGSSAAAAGVAGQEHLLPDLDVFNLGNSSGVKRKSLSGSVALGSEPELQEEENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
G+++P IEEP +P+L W E + SG+EDSLL + AASIS V S T ++ ERR+NAL LK +CE+ V APHL+ LL +DAQ TP M A+ RAY AAL+L + Q+V D K +M+ P + PD SPL V CCNDTCSFTWTGAEHINQDIFECRTCGLT SLCCCTECARVCH+GHDCKLKKTSPTAYCDCWEKCKCR+LI GHQGARY++LC+L+ +TDL + N RGE+ILLFLVQTVGRQ EQRQ+R P + S SRKTP D DMP+HDL+PPRFSRRAL+ LL DW A++ MIM+G E N ++ DQ QSGT LLDKFTH LLV+CS + LD LLT LIR LQ S G EA VA+RFVRSV R+FV+F +EMAP+ +K++ + SQPL +CRRVFQALI +A+EELCETA++L+APVR G++RPTAPF+L+ S V EELFS+EP++P + +S Q + + +++ + R VS + M+ + H E+ +G A L GEV+ + G V D SD +LDLLA ET+S+SDD N R ++G ++ G LF +D+SGES+ ED ES+AGETDEQD+ +F ++EQLERR G+T R+NLAP SMQWA+R+R S+RT + ++IDP++LRR+T++ A E +TM TTAS LARAFGIVIRQIADLLTM+QDY +LAP L R +EI+YQ++ LQ+ +E H+KP WDWL+ VMD+TEAQLRFG +L+ S D ++P N P G T G R G D RR+FL+Y LSLMRAHN EH DSLPVLDV++++H+AYVFDAL+YYMRS + DAQ++S S S +++ + +D G AT ME D D + T +KGR+H F QRSDSTLCLGCPP DPF T M EALPLADQPHLLQP++RREDLFGVP+QP +GP N L LPT LGLS R TD + V P F+ + P + + D+ +V +P S T + T V V+ + +A T + +D+ G + A +VSE V APEVLVVPT E+ D ++SA+ T+ETS +AR V+S+G ++ H+ILLGRWR +LDLFGRVF++DVGLE GS++SELGGFPVKE KFRR+MEKLRNS+ D+T++K+ER+R QL+ Q KE N+ N + RR+S++ PPL VNR+KVTF++EPGEGSGV RSFYTA++EALL++ KLPNLE AQ G SK + ++Q+L+ SRD D R QN + R+ RAL+ EAR F+ L SNDHL+ Q QLG RL+P+V ALR LA KITGMLLEL PAQLL+LLASE++LRQ+VEEA ELI S + +AS A L DLDVF+L G +K + S+ ++ EDNAPLFY+PGKRGFY+P QGR + ER+NAFRNVGRL+GLCLLQNELCP+F NRHVIKY+LGR IRFHDLAFFD VIYESLRQLV+DAE KD+++ LFS L+LTFSIDL EEGGGS ELVPNGRD+EV A+NVY+YVRKY++ RM QEKALE M+ G+FDVLP G+LD LT+ED RLLLNGVGDINV LISYTSFNDESGE+ +RLV FKRWLW IVEKMS +E+QDLVYFWTGSPALPASEDGFQPMPSVTIRPA D+HLP+ANTCISRLYVPLYSS+ +L+ KLL+AIKTKNFGFV
Sbjct: 953 GNSTP--IEEP-IPTLSWSAEAFDNTSGEEDSLLSIG--AASISMMNKTGVGSASNNTYIVDSVERRNNALMILKYMCENPVLAPHLKDLLVARDAQGQTPLMLAVSVRAYHAALILLDTIQRVGRD--------GKDCSAMILPSDASPDLSPLFVTCCNDTCSFTWTGAEHINQDIFECRTCGLTGSLCCCTECARVCHRGHDCKLKKTSPTAYCDCWEKCKCRALIAGHQGARYDLLCRLVTNTDLATKINSRGESILLFLVQTVGRQSVEQRQFRSAPRQRSTSASRKTPSSDVFLSGLGADLDMPDHDLEPPRFSRRALERLLNDWPAVQCMIMSGVSE-----NSNDQLFGDQGQSCRQSGTALLDKFTHTLLVKCSAEMLDTLLTTLIRELQNESVPGRQEEANHVARRFVRSVARIFVIFTVEMAPNTTKRRGVSQSSQPLMKCRRVFQALIKLAVEELCETADSLIAPVRLGVARPTAPFTLTSSTIEVINGSEELFSIEPLIPHSGVSSQILDAALQTQQGNNNITIARDVSAIDEAEGGEEVPMDMDGDISEH-----EESGVLGAVAGQPL-GEVDSNAGGVGEEQTGDGESDTELDLLAEPETESDSDDNHSNQDAASAQRSVQTGATAGSDGGMGSILLFPEDESGESSQQEDDESEAGETDEQDNDEFQIADEQLERRRFVIIRFSGSTSHSHRNNLAPVSMQWAIRNRESSTRTAGLRMASSNNLVFIDPSSLRRSTATSAVAAA---QEPITMGTTASCLARAFGIVIRQIADLLTMMQDYKTLAPALPRLIEITYQDATTLQMYLEGHLKPTWDWLLTVMDATEAQLRFGVSLTRSADPTHPEHPLNNAPSLSGGNFTGLLNSAALSLTLQGNTGVGEPPRNNRDREGGDANSARREFLSYCLSLMRAHNGEHRDSLPVLDVSALRHVAYVFDALVYYMRSLSEERDAQKES-----------STYSTWNDQDENDNEEGEEDNLPAATAMETDSVDYPDLLQVPICGTGNNNNTTAKGRKHPFLQRSDSTLCLGCPPLDPFDTVMSEALPLADQPHLLQPHSRREDLFGVPRQPTATPGTTGPNQ--NPLEGLPTWLGLSVRNTDNQNPS--VTP--TFSQVVQGPGPSG--SSDNRRSSVCSNEPGPSKCMEKPKSFNQTTDNQQPMITEQVDRAPIIVSPSSQNDAGTAKGKDICKNGRSVIVRAGTVSEPSVNKV----------------------GAPEVLVVPTVEAQNVSEEVDPAASSHEISAHETVETSPVESARAVSSIGPNISHNILLGRWRLSLDLFGRVFMEDVGLEAGSVVSELGGFPVKEVKFRRDMEKLRNSQQKDITLSKVERDRTQLLVQTMKELNTQYNLYNRRASNT-PPLAVNRIKVTFKDEPGEGSGVTRSFYTAIAEALLANEKLPNLEAAQVG-SKYTQYNVLQKLK-SRDRDRDLRRQNPRSSGKCRETRRALSLEARSFHPTVSVEGASGSGPGSSSSSAHPLPVSHPSNDHLTMQQEQLGNRLYPKVHALRSTLAEKITGMLLELPPAQLLMLLASEDALRQKVEEAFELIHSRNQD------------LASEA-------------------------------------LLDLDVFSLTERCGANKKKMENSIL----------DDTEDNAPLFYSPGKRGFYTPRQGRASYERLNAFRNVGRLIGLCLLQNELCPIFLNRHVIKYILGRPIRFHDLAFFDSVIYESLRQLVIDAETKDSNS-LFSTLDLTFSIDLCPEEGGGSIELVPNGRDMEVTASNVYDYVRKYAEVRMIKVQEKALEAMREGVFDVLPDGALDGLTSEDLRLLLNGVGDINVSVLISYTSFNDESGESTDRLVKFKRWLWSIVEKMSHVERQDLVYFWTGSPALPASEDGFQPMPSVTIRPADDAHLPTANTCISRLYVPLYSSRHVLRHKLLLAIKTKNFGFV 2818
BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Match: gi|1009415966|gb|KYN34767.1| (E3 ubiquitin-protein ligase hyd [Trachymyrmex septentrionalis]) HSP 1 Score: 1800.02 bits (4661), Expect = 0.000e+0 Identity = 1014/1989 (50.98%), Postives = 1296/1989 (65.16%), Query Frame = 0
Query: 221 DASPPVIEEPSLPSLPWPPEPMEGNSGDEDSLLGLAGSAASISSHKSVL--VKERSPATENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYR--PSRSRKSTSRKTPELD-----QDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQ---VGSDKGEARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSNDSV--SIEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEVNDD-GPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG---------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRSRSKSSRT----VNGG--FIYIDPNALRRTTSSGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTPGRTRTGFQSRAGSLA--------------SGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMR-------SGTDAQEDSPFGMDPTPMVPSAASNFDENEND-DEAAGSGTPA-TNMEVDDEDTNQSAAI-------LAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMTNTLAVLPTRLGLSARTTDEAGATSAVQPAEAFNAMSTAPFQATPITGDSNFENVVRQARPSSPTAATCDTASVRSLDTTVTGGNSEEAETDQPQDLSMGGGGTMSSASSVSEEP-VASTSKGSFTSPKK--------------AFMMREAARGASSAPEVLVVPT-------EKPDQG----DVSANVTIETS---AARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRL----RGSRDAQIDRR------IQNRFYGRRSRDAARALNFEARPFY-LNGVGG--------------------SNDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGSSGSSAAAAGVAGQEHLLPDLDVFNLGNSSGVKRKSLSGSVALGSEPELQEEENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
D + IEEP +P+L WPPE + SG+EDSLL + AASIS KS + + ++ ERR NAL LK +CE+ + PHL LL +D Q TP M A+ RAY AAL+L + Q+V D K M+ P + PD SPL V CCNDTCSFTWTGAEHINQDIFECRTCGLT SLCCCTECARVCH+GHDCKLK+TSPTAYCDCWEKCKCR+LI GHQGARY++LC+L+ TDL + N RGE+ILLFLVQTVGRQL EQRQ+R P + S SRK P D DMP+HDL+PPRFSR+AL+ LL DW A++ MIM+G E NGT ++ DQ L QSGT LLDKFTH LLV+CS + LD LLT LIR LQ V + EA VA+RFVRSV R+FV+F IEMAP+ +K++S SQPL +CR+VFQALI +A+EELCETA++L+APVR G++RPTAPF+L+ S V EELFS+EP++P + +S Q ++ ++ + + + ++S+ + A++ A +++ +E+ + A + GEV+ + G V D SD +LDLLAE ++ESD D +++DA S ++ G LF +D+SGES+ ED ES+AGETDEQD+ +F +EQLERR+ R+NLAP MQWA+R+R ++RT V GG ++IDP +LRRTT++ E +TM TTAS LARAFGIVIRQIADLL M+QDY +LAP L R +EI++Q+++NLQ +E H+KP WDWL+ VMD+TEAQLRFG +L+ S D ++P N TP + F + A G D RR+FL+Y LSLMRAHN+EH DSLPVLDV++++H+AYVFDAL+YYMR S ++ Q++S S +S D++END DE +PA T ME D D + + +A KGR+H F QRSDSTLCLGCPP DPF T M EALPLADQPHLLQP++RREDLFG+P+QP G S N L LPTRLGLSAR D +T V P F+ + P +SN + +R +S + +++S++ + ++ E G ++ + + P + ST++G T+ K A M E+ APE+LVVPT E+ D ++SA+ T+ETS +AR V+S+G ++ H+ILLGRWR +LDLFGRVF++DVGLE GS++SELGGFPVKEAKFRR+MEKLRNS+ D+TI K+ER+R QL+ Q KE N+ N + RR+SS+ PPL V+RVKV F++EPGEG+GV RSFYTA++EALL++ KLPNLE AQ SK + ++Q+L R D+ +D R QN + R+ RAL+F+AR F+ N + G +NDHL+ +Q QLGERL+P+V A+RP LA KITGMLLELS +QLLLLLASEE+LRQRVEEA +LI +H +++ + DLDVF+L +K + S+ ++ EDNAP+FY+PGKRGFY+P QGRG+ ERMNAFRNVGRL+GLCLLQNELCPLF NRHVIKY+LGR IRFHDLAFFD VIYESLRQLV+DAE KD+++ LFSAL+LTFSIDL EEGGGS ELV NGRD+EV A+NVY+YVRKY++ RM QEKAL +M+ G+FDVLP G+LD LT+ED RLLLNGVGDINV LISYTSFNDESGE+ ERLV FKRWLW IVEKMS +E+QDLVYFWTGSPALPASEDGFQPMP+VTIRPA D+HLP+ANTCISRLYVPLYSS+ +L+ KLL+AIK+KNFGFV
Sbjct: 960 DGTSAPIEEP-IPTLSWPPEAFDNTSGEEDSLLSIG--AASISMMKSGVGATSNNTYIIDSVERRHNALYILKYMCENNILIPHLRELLTAKDGQGQTPLMLAVSVRAYHAALILLDTIQRVGKDA--------KDTSVMILPADASPDLSPLFVTCCNDTCSFTWTGAEHINQDIFECRTCGLTGSLCCCTECARVCHRGHDCKLKRTSPTAYCDCWEKCKCRALIAGHQGARYDLLCRLVNSTDLATKINSRGESILLFLVQTVGRQLVEQRQFRSAPRQRSTSASRKAPTSDGLGAESDMPDHDLEPPRFSRKALERLLNDWPAVQCMIMSGVSE-NGTD----QLFGDQGQLCRQSGTALLDKFTHSLLVKCSAEMLDTLLTTLIRELQDETVPGRQEEANNVARRFVRSVARIFVIFTIEMAPNTTKRRSTSQASQPLMKCRKVFQALIKLAVEELCETADSLIAPVRLGVARPTAPFTLTSSAIEVINGSEELFSIEPLIPHSGVSSQTLDA-ALQTQQGNNNI--TISRGDVSAVD-EAEGGEEVPMDMDGDMSEHEESGVSGTATNQPLGEVDSNAGGVGEEQAGDGESDTELDLLAEAETESDSDDNHSNQDAASAQRSVQTGATAGSDGGMGSILLFPEDESGESSQQEDDESEAGETDEQDNEEFQIGDEQLERRSGTSGHLHRNNLAPVYMQWAIRNRESTTRTAGLRVTGGSNLVFIDPASLRRTTATSAVATA---QEPITMGTTASCLARAFGIVIRQIADLLVMMQDYKNLAPALPRLMEITFQDALNLQSYLEAHLKPTWDWLLTVMDATEAQLRFGVSLTRSADPTHPEHPLNNTPSLSGGNFSGLLNTAALSLTLQGNTNRNPRGGGDAHSARREFLSYCLSLMRAHNSEHRDSLPVLDVSALRHVAYVFDALVYYMRSLSEPLASRSETQKES----------SSYSSWNDQDENDNDEGEEYNSPAPTAMETDSVDYPDLLQVPICGSGNHSNSAPKGRKHPFLQRSDSTLCLGCPPLDPFDTVMSEALPLADQPHLLQPHSRREDLFGIPRQPTCSNVGG--SSQNPLEGLPTRLGLSARGMDSQNST--VTP--TFSQVVQGP--------NSNID-----SRRNSISVGDLNSSSIKYAEKLKLSTHANE------------GHSSLVTEQIIDRAPIIVSTNQGDATTSIKGKDMCKNNRSVIVRAGTMSESNTNKVGAPEILVVPTVESQNVSEEVDPAATSHEISAHETVETSPVESARTVSSIGTNISHNILLGRWRLSLDLFGRVFMEDVGLEAGSVVSELGGFPVKEAKFRRDMEKLRNSQQKDITI-KVERDRTQLLVQTMKELNAQYNIYNRRASSNTPPLAVSRVKVFFKDEPGEGAGVTRSFYTAIAEALLANEKLPNLEAAQI-ESKYTQYNVMQKLKSRDRDLTDSSLDSRHMNIVSFQNTRSSGKCRETRRALSFDARSFHPSNTIEGNNSSTPGSSSSTHPLPINHPNNDHLTTNQQQLGERLYPKVHAIRPALAEKITGMLLELSSSQLLLLLASEEALRQRVEEAFDLIHTHTQDLTSE--------------------------------------------------MLDLDVFSLTERCAASKKKMENSIL----------DDTEDNAPIFYSPGKRGFYTPRQGRGSYERMNAFRNVGRLIGLCLLQNELCPLFLNRHVIKYILGRPIRFHDLAFFDSVIYESLRQLVIDAETKDSNS-LFSALDLTFSIDLCPEEGGGSIELVHNGRDMEVTASNVYDYVRKYAEVRMIKVQEKALHSMREGVFDVLPEGALDGLTSEDLRLLLNGVGDINVSVLISYTSFNDESGESTERLVKFKRWLWSIVEKMSHVERQDLVYFWTGSPALPASEDGFQPMPTVTIRPADDAHLPTANTCISRLYVPLYSSRHVLRHKLLLAIKSKNFGFV 2821
BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Match: gi|572311965|ref|XP_006621705.1| (PREDICTED: E3 ubiquitin-protein ligase hyd-like isoform X3 [Apis dorsata]) HSP 1 Score: 1778.07 bits (4604), Expect = 0.000e+0 Identity = 989/1968 (50.25%), Postives = 1262/1968 (64.13%), Query Frame = 0
Query: 227 IEEPSLPSLPWPPEPMEGNSGDEDSLLGLAGSAASI--SSHKSV-LVKERSPATENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKST--SRKTPELDQ------DMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQVGSDKG---EARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSNDSV--SIEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEV---------NDDGPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG---------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRSRSKSSRT----VNGG--FIYIDPNALRRTTSSGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNP-NNPTP--------------------GRTRTGFQSRAGSLASGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRS--------GTDAQEDSPFGMDPTPMVPSAASNFDENENDDEAAGSGTPATNMEVDDEDTNQSAAILA-------ATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMTNTLAVLPTRLGLSARTTDEAG----ATSAVQPAEAFNAMSTAPFQATPITGDSNFENVVR-QARPSSPTAATCDTASVRSLDTTVTGGNSEEAETDQPQDLSMGGGGTMSSASSVSEEPVASTSKGSFTSPKKAFMMREAARGASSAPEVLVVPTEKPDQGDVSANVTI--ETSAARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFYGRRSRDAARALNFEARPFY-LNGVGGS---------------------NDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGSSGSSAAAAGVAGQEHLLPDLDVFNLGNSSGVKRKSLSGSVALGSEPELQEEENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
IEEP +P+L WPPE + SG+EDSLL + ++ S+ S KSV + + ++ ERR+NAL LK +CES V APHL+ LL +D Q TP M A+ RAY AAL+L + QKV D +K +M+ P + PD SPL V CCNDTCSFTWTG +HINQDIFEC+TCGL SLCCCTECARVCH+GHDC++K TSPTAYCDCWEKCKCR+LI GHQGARYE+LC+L+ +TDLV + N RGE+ILLFLVQTVGRQL EQRQYR + ++ST SRK P D DMP+HDL+PPRF+R+AL+ LL DW A++ MIM+G NG N ++ DQ L QSGT LLDKFTH LL +CS + LD LLT LIR LQ + G EA VA+RFVRSV R+FV+F IEMAP+ +K++S QPL +CR+VFQALI +A+EELCETA++L+APVR G++RPTAPF+L+ S V EELFS+EP++P + +S Q ++ Q N+ SAM D E ++ + + I E E G+ G V ++ G V D SD +LDLLAE ++ESD D +++DA S ++ G LF +D+SGES+ ED ES+AGETDEQD+ DF ++QLERR+ R+NLAP SMQWA+R+R S RT V GG ++ID +LRRTT++ A E + M T+ + LARAFGIVIRQIADLL M+QDY +LAP L R +EI+YQ+++NLQ+ +E H+KP WDWL+ VMD+TEAQLRFG +L+ S D ++P NN + T G SR G D RR+FL+Y LSLMRAHNAEH DSLPVLDV++++H+AYVFDAL+YYMRS G +E S + S+ ++ DEN+ND+ S T +E D D + ++ KGR+H F QRSDSTLCLGCPP DPF T M EALPLADQPHLLQP+++REDLFG+P+QP ++GP N L LPTRL LSAR D S + AF + T T GDS+ +++ + ++ + A D A + + +T + T +D+ + A +VSE TS A+ S++ E + TE+ D + +I ET V+S+G ++ H+ILLGRW +LDLFGRVF++DVGLE GS++SELGGF VKEAKFRR+MEKLRN++ D+ + K+ER+R QL+ Q KE N+H N + RR++++ PPL V+RVKVTF++EPGEG+GV RSFYTA++EALL++ KLPNLE AQ G SK + ++++L+ SRD D R QN + R+ R L+FEAR F+ + V G+ NDHL+ HQ Q G+RL+ +V AL+P LA KITGMLLEL PAQLL+LLASE++L+Q+VEEA E+I SH S SA A L DLDVF+L G +K + S+ ++ EDNAPLFY+PGKRGFY+P QG+ + ER+NAFRNVGRL+GLCLLQNELCP+F NRHVIKY+LGR IRFHDLAFFD VIYESLRQLV+D+E KD+++ LFSAL+LTFSIDL EEGGGS EL+ NGRDIEV A NVY+YVRKY++ RM Q+KAL M+ G+FDVLP G+LD LT+ED RLLLNGVGDINV LISYTSFNDESGE +RL FKRWLW IVEKMS E+QDLVYFWTGSPALPASEDGFQPMPSVT+RPA D HLP+ANTCISRLY+PLYSS+ IL+ KLL+AI+TKNFGFV
Sbjct: 956 IEEP-IPTLSWPPEAFDNTSGEEDSLLSIGTASISMMNKSGKSVGVTSNNTYIIDSVERRNNALLILKYMCESSVLAPHLKELLTAKDIQGQTPLMLAVSVRAYHAALILLDIIQKVGRD--------QKECSAMILPADANPDLSPLFVTCCNDTCSFTWTGTQHINQDIFECKTCGLIGSLCCCTECARVCHRGHDCRIKITSPTAYCDCWEKCKCRALIAGHQGARYELLCRLVVNTDLVTKINSRGESILLFLVQTVGRQLIEQRQYRSALRQRSTSASRKGPSSDGLDTYVPDMPDHDLEPPRFNRKALERLLNDWPAVQCMIMSG-VSVNG----NEQLFGDQGQLCRQSGTALLDKFTHSLLFKCSAEMLDTLLTTLIRELQNDTIPGRQEEANNVARRFVRSVARIFVIFTIEMAPNTTKRRSTSQALQPLMKCRKVFQALIKLAVEELCETADSLIAPVRLGVARPTAPFTLTSSAMEVINGSEELFSIEPLIPHSGVSSQTLDA------------TLQTQQGNNNITIARDVSAM-DEAEAGEEVPMDVDGDISEHEESGVSGTVAGQPLGEIDSNGGGVGEEQAGDGESDTELDLLAEAETESDSDDNHSNQDAASAQRSVQTGATAGSDGGMGSILLFPEDESGESSQQEDDESEAGETDEQDNEDFQIGDDQLERRSGSSGHLHRNNLAPVSMQWAIRNRDSSMRTAGLRVTGGSNLVFIDSASLRRTTATSAVAAA---QEPIIMGTSTTCLARAFGIVIRQIADLLVMMQDYKTLAPSLPRLMEITYQDALNLQMYLEGHLKPTWDWLLTVMDATEAQLRFGVSLTRSADPTHPEHPLNNASSLSGGNFSGLLNTAALSLTLQSNTGVGEPSRNSREREGGDVYLARREFLSYCLSLMRAHNAEHRDSLPVLDVSALRHVAYVFDALVYYMRSLSEPISSRGESQKEPSNY---------SSWNDQDENDNDEGEEYSSPVPTALETDSVDYPDLLQVPICGPGSNNSSTPKGRKHPFLQRSDSTLCLGCPPLDPFDTVMSEALPLADQPHLLQPHSKREDLFGIPRQPTA--NAGPNQ--NPLEGLPTRLSLSARGADNQNTPTPTFSQIIQRSAFASSDTRRNSVT--IGDSSAKHMEKAKSFNHTLVAEQIDRAPI--IVSTNNQSDQVTGNTKSNKDICKINRSVIVRAGTVSEPSSNKTS---------------ASEMMSTSNETIENTTEEMDTSSSNQETSISHETVETNPVSSIGTNISHNILLGRWSLSLDLFGRVFMEDVGLEAGSVVSELGGFQVKEAKFRRDMEKLRNAQQRDINLLKVERDRTQLLVQTMKELNTHYNLYNRRATNA-PPLAVHRVKVTFKDEPGEGTGVTRSFYTAIAEALLANEKLPNLEAAQVG-SKYTQYNVLRKLK-SRDRDHDLRRQNPRSSGKCRETRRTLSFEARVFHPSSSVEGTSNSGAGSSSSNSHPLPVSHPNNDHLTMHQLQFGDRLYSKVYALQPALAAKITGMLLELPPAQLLMLLASEDALQQKVEEAFEIIRSH-----------------------------------------SQESAKEA--------LLDLDVFSLIARCGANKKKIENSIL----------DDTEDNAPLFYSPGKRGFYTPRQGKASYERLNAFRNVGRLIGLCLLQNELCPIFLNRHVIKYILGRPIRFHDLAFFDSVIYESLRQLVIDSETKDSNS-LFSALDLTFSIDLCPEEGGGSIELISNGRDIEVTANNVYDYVRKYAEVRMIKVQDKALHAMREGVFDVLPEGALDGLTSEDLRLLLNGVGDINVSVLISYTSFNDESGEPADRLAKFKRWLWSIVEKMSHSERQDLVYFWTGSPALPASEDGFQPMPSVTLRPA-DDHLPTANTCISRLYLPLYSSRHILRHKLLLAIQTKNFGFV 2797
BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Match: gi|1035603217|ref|XP_016909078.1| (PREDICTED: E3 ubiquitin-protein ligase hyd [Apis cerana]) HSP 1 Score: 1777.68 bits (4603), Expect = 0.000e+0 Identity = 982/1965 (49.97%), Postives = 1256/1965 (63.92%), Query Frame = 0
Query: 227 IEEPSLPSLPWPPEPMEGNSGDEDSLLGLAGSAASISSHKSV-LVKERSPATENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKST--SRKTPELDQ------DMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQVGSDKG---EARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSNDSV--SIEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEV---------NDDGPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG---------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRSRSKSSRT----VNGG--FIYIDPNALRRTTSSGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNP-NNPTP--------------------GRTRTGFQSRAGSLASGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRS--------GTDAQEDSPFGMDPTPMVPSAASNFDENENDDEAAGSGTPATNMEVDDEDTNQSAAI-------LAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMTNTLAVLPTRLGLSARTTDEAGATSAVQPAEAFNAMSTAPFQATPITGDSNFENVVRQARPSSPTAATCDTASVRSLDTTVTGGNSEEA----ETDQPQDLSMGGGGTMSSASSVSEEPVASTSKGSFTSPKKAFMMREAARGASSAPEVLVVPTEKPDQGDVSANVTI--ETSAARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFYGRRSRDAARALNFEARPFY-LNGVGGS---------------------NDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGSSGSSAAAAGVAGQEHLLPDLDVFNLGNSSGVKRKSLSGSVALGSEPELQEEENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
IEEP +P+L WPPE + SG+EDSLL + ++ S+ + V + + ++ ERR+NAL LK +CES V APHL+ LL +D Q TP M A+ RAY AAL+L + QKV D +K +M+ P + PD SPL V CCNDTCSFTWTG +HINQDIFEC+TCGL SLCCCTECARVCH+GHDC++K TSPTAYCDCWEKCKCR+LI GHQGARYE+LC+L+ +TDLV + N RGE+ILLFLVQTVGRQL EQRQYR + ++ST SRK P D DMP+HDL+PPRF+R+AL+ LL DW A++ MIM+G NG N ++ DQ L QSGT LLDKFTH LL +CS + LD LLT LIR LQ + G EA VA+RFVRSV R+FV+F IEMAP+ +K++S QPL +CR+VFQALI +A+EELCETA++L+APVR G++RPTAPF+L+ S V EELFS+EP++P + +S Q ++ Q N+ S M++T E ++ + + I E E G+ G V ++ G V D SD +LDLLAE ++ESD D +++DA S ++ G LF +D+SGES+ ED ES+AGETDEQD+ DF ++QLERR+ R+NLAP SMQWA+R+R S RT V GG ++ID +LRRTT++ A E + M T+ + LARAFGIVIRQIADLL M+QDY +LAP L R +EI+YQ+++NLQ+ +E H+KP WDWL+ VMD+TEAQLRFG +L+ S D ++P NN + T G SR G D RR+FL+Y LSLMRAHNAEH DSLPVLDV++++H+AYVFDAL+YYMRS G +E S + S+ ++ DEN+ND+ S T +E D D + +++ KGR+H F QRSDSTLCLGCPP DPF T M EALPLADQPHLLQP+++REDLFG+P+QP ++GP N L LPTRL LSAR GA + P F+ + Q + N V +S T +S + T+ + A T+ D + G + ++ + S S K A+ S++ E + TE+ D + +I ET V+S+G ++ H+ILLGRW +LDLFGRVF++DVGLE GS++SELGGF VKEAKFRR+MEKLRN++ D+ + K+ER+R QL+ Q KE N+H N + RR++++ PPL V+RVKVTF++EPGEG+GV RSFYTA++EALL++ KLPNLE AQ G SK + ++++L+ SRD D R QN + R+ R L+FEAR F+ + V G+ NDHL+ HQ Q G+RL+ +V AL+P LA KITGMLLEL PAQLL+LLASE++L+Q+VEEA E+I SH S SA A L DLDVF+L G +K + S+ ++ EDNAPLFY+PGKRGFY+P QG+ + ER+NAFRNVGRL+GLCLLQNELCP+F NRHVIKY+LGR IRFHDLAFFD VIYESLRQLV+D+E KD+++ LFSAL+LTFSIDL EEGGGS EL+ NGRDIEV A NVY+YVRKY++ RM Q+KAL M+ G+FDVLP G+LD LT+ED RLLLNGVGDINV LISYTSFNDESGE +RL FKRWLW IVEKMS E+QDLVYFWTGSPALPASEDGFQPMPSVT+RPA D HLP+ANTCISRLY+PLYSS+ IL+ KLL+AI+TKNFGFV
Sbjct: 956 IEEP-IPTLSWPPEAFDNTSGEEDSLLSIGTASISMMNKSGVGVTSNNTYIIDSVERRNNALLILKYMCESTVLAPHLKELLTAKDIQGQTPLMLAVSVRAYHAALILLDIIQKVGRD--------QKECSAMILPADANPDLSPLFVTCCNDTCSFTWTGTQHINQDIFECKTCGLIGSLCCCTECARVCHRGHDCRIKITSPTAYCDCWEKCKCRALIAGHQGARYELLCRLVVNTDLVTKINSRGESILLFLVQTVGRQLIEQRQYRSALRQRSTSASRKGPSSDGLDTYVPDMPDHDLEPPRFNRKALERLLNDWPAVQCMIMSG-VSVNG----NEQLFGDQGQLCRQSGTALLDKFTHSLLFKCSAEMLDTLLTTLIRELQNDTIPGRQEEANNVARRFVRSVARIFVIFTIEMAPNTTKRRSTSQALQPLMKCRKVFQALIKLAVEELCETADSLIAPVRLGVARPTAPFTLTSSAMEVINGSEELFSIEPLIPHSGVSSQTLDA------------TLQTQQGNNNITIARDVSTMDET-EAGEEVPMDVDGDISEHEESGVSGTVAGQPLGEIDSNGGGVGEEQAGDGESDTELDLLAEAETESDSDDNHSNQDAASAQRSVQTGATAGSDGGMGSILLFPEDESGESSQQEDDESEAGETDEQDNEDFQIGDDQLERRSGSSGHLHRNNLAPVSMQWAIRNRDSSMRTAGLRVTGGSNLVFIDSASLRRTTATSAVAAA---QEPIIMGTSTTCLARAFGIVIRQIADLLVMMQDYKTLAPSLPRLMEITYQDALNLQMYLEGHLKPTWDWLLTVMDATEAQLRFGVSLTRSADPTHPEHPLNNASSLSGGNFSGLLNTAALSLTLQSNTGVGEPSRNSREREGGDVYLARREFLSYCLSLMRAHNAEHRDSLPVLDVSALRHVAYVFDALVYYMRSLSEPISSRGESQKEPSNY---------SSWNDQDENDNDEGEEYSSPVPTALETDSVDYPDLLQVPICGPGSNSSSTPKGRKHPFLQRSDSTLCLGCPPLDPFDTVMSEALPLADQPHLLQPHSKREDLFGIPRQPTA--NAGPNQ--NPLEGLPTRLSLSAR-----GADNQNTPTPTFSQI----IQRSAFASSDARRNSVTIGDSTSAK----HTEKAKSFNHTLVAEQIDRAPIIVSTNNQSDQATGNIKSNKDICKINRSVIVRAGTVSEPSSNKT----NASEMMSTSNETIENATEEMDTSGSNQETSISHETIETNPVSSIGTNISHNILLGRWSLSLDLFGRVFMEDVGLEAGSVVSELGGFQVKEAKFRRDMEKLRNAQQRDINLLKVERDRTQLLVQTMKELNTHYNLYNRRATNA-PPLAVHRVKVTFKDEPGEGTGVTRSFYTAIAEALLANEKLPNLEAAQVG-SKYTQYNVLRKLK-SRDRDHDLRRQNPRSSGKCRETRRTLSFEARVFHPSSSVEGTSNSGAGSSSSNSHPLPVSHPNNDHLTMHQLQFGDRLYSKVYALQPALAAKITGMLLELPPAQLLMLLASEDALQQKVEEAFEIIRSH-----------------------------------------SQESAKEA--------LLDLDVFSLIARCGANKKKIENSIL----------DDTEDNAPLFYSPGKRGFYTPRQGKASYERLNAFRNVGRLIGLCLLQNELCPIFLNRHVIKYILGRPIRFHDLAFFDSVIYESLRQLVIDSETKDSNS-LFSALDLTFSIDLCPEEGGGSIELISNGRDIEVTANNVYDYVRKYAEVRMIKVQDKALHAMREGVFDVLPEGALDGLTSEDLRLLLNGVGDINVSVLISYTSFNDESGEPADRLAKFKRWLWSIVEKMSHSERQDLVYFWTGSPALPASEDGFQPMPSVTLRPA-DDHLPTANTCISRLYLPLYSSRHILRHKLLLAIQTKNFGFV 2796
BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Match: gi|805752573|ref|XP_012151366.1| (PREDICTED: E3 ubiquitin-protein ligase UBR5 isoform X5 [Megachile rotundata]) HSP 1 Score: 1773.06 bits (4591), Expect = 0.000e+0 Identity = 1003/1968 (50.97%), Postives = 1271/1968 (64.58%), Query Frame = 0
Query: 227 IEEPSLPSLPWPPEPMEGNSGDEDSLLGLAGSAASISSHKSVLVKERSPAT---ENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKST--SRKTPELD-----QDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQ---VGSDKGEARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSNDSV--SIEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEV---------NDDGPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG---------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRSRSKSSRT----VNGG--FIYIDPNALRRTTSSGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTP---GRTRTG----------FQSRAG----SLAS----GQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRS--------GTDAQEDSPFGMDPTPMVPSAASNFDENENDDEAAGSGTPATNMEVDDEDTNQSAAI-------LAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMTNTLAVLPTRLGLSARTTDEAGATS--AVQPAEAFNAMSTAPFQATPITGDSNFENVVRQARP-SSPTAATCDTASVRSLDTTVTGGNSEE---AETDQPQDLSMGGGGTMSSASSVSEEPVASTSKGSFTSPKKAFMMREAARGASSAPEVLVVPTEKPDQGD--VSANVTIETSAARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFYGRRSRDAARALNFEARPFY----------------------LNGVGGSNDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGSSGSSAAAAGVAGQEHLLPDLDVFNLGNSSGVKRKSLSGSVALGSEPELQEEENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
IEEP +P+L WPPE + SG+EDSLL + ++ S+ + V S T ++ ERR+NAL LK +CES V APHL+ LL +D Q TP M A+ RAY AAL+L + Q+V D +K +M+ PP + PD SPL V CCNDTCSFTWTGA+HINQDIFECRTCGLT+SLCCCTECARVCH+GHDCK+K TSPTAYCDCWEKCKCR+LI GHQGARYE+LC+L+ +TDL + N RGE+ILLFLVQTVGRQL EQRQ R + ++ST SRK P D +MP+HDL+PPRFSR+AL+ LL DW A++ MIM+G + N ++ DQ L QSGT LLDKFTH LLV+CS + LD LLT LIR LQ V + EA VA+RFVRSV R+FV+F IEMAP+ +K++++ SQPL +CR+VFQALI +A+EELCETA++L+APVR G++RPTAPF+L+ S V EELFS+EP++P + +S Q ++ Q N+ SAM++T + + + I E E G+ G V ++ G V D SD +LDLLAE ++ESD D +++DA S ++ G LF +D+SGES+ ED ES+AGETDEQD+ DF +EQLERR+ R+NLAP SMQWA+R+R ++RT V GG +ID +LRRTT++ A E + M TT S LARAFGIVIRQIADLL M+QDY AP L R +EISYQ+++NLQ+ +E H+KP WDWL+ VMD+TEAQLRFG +L+ S D ++P N P G TG QS AG S +S G D RR+FL+Y LSLMRAHN EH DSLPVLDV++++H+AYVFDAL+YYMRS G +E S + S+ ++ DEN+ND+ + T +E D D + + KGR+H F QRSDSTL LGCPP DPF T M EALPLADQPHLLQP++RREDLFG+P+Q +SG S N LA LPTRLGLSAR TD +S + Q + +S+ +++ GDS +A+ S P T + V+ N E T +D+ + A +VSE ++ +K + MM S+ E++ T+ P +A T+ET+ + ++G ++ H+ILLGRWR +LDLFGRVF++DVGLE GS++SELGGF VKEAKFRR+MEKLRNS+ D+ + K+ER+R QL+ Q KE N+ N + RR+S++ PPL VNRVKV F++EPGEGSGV RSFYTA++EALL++ KLPNLE AQ G SK + ++Q+L+ SRD + R QN + R+ RAL+FEAR F+ L SN+HL+ HQ QLG+RL+P+V ALRP LA+KITGMLLEL PAQLL+LLASE++LRQ+VEEA ELI SH A +E LL DLDVF+L G +K + S+ ++ EDNAPLFY+PGKRGFY+P QGR + ER+NAFRNVGRL+GLCLLQNELCP+F NRHVIKY+LGRSIRFHDLAFFD VIYESLRQLV D+E KD+++ LFS L+L FSIDL EEGGGS EL+PNGRDIEV A+NVY+YVRKY++ RM QEKAL M+ G+FDVLP GSLD LT+ED RLLLNGVGDINV LISYTSFNDESGE+ +RL FKRWLW IVEKM+ E+QDL+YFWTGSPALPASEDGFQPMP+VT+RPA D+HLP+ANTCISRLYVPLYSS+ +L+ KLL+AIKTKNFGFV
Sbjct: 956 IEEP-IPTLSWPPEAFDNTSGEEDSLLSIGAASISMMNKSGEGVGATSNNTYIIDSVERRNNALLILKYMCESSVLAPHLKELLTAKDVQGQTPLMLAVSVRAYHAALILLDTIQRVGRD--------QKECSAMILPPDANPDLSPLFVTCCNDTCSFTWTGADHINQDIFECRTCGLTESLCCCTECARVCHRGHDCKMKITSPTAYCDCWEKCKCRALIAGHQGARYELLCRLVVNTDLATKINSRGESILLFLVQTVGRQLIEQRQCRSAPRQRSTSGSRKGPSSDGLTAEANMPDHDLEPPRFSRKALERLLNDWPAVQCMIMSG-----VSVNSNDQLFGDQGQLCRQSGTALLDKFTHSLLVKCSAEMLDTLLTTLIRELQNDTVPGRQEEANNVARRFVRSVARIFVIFTIEMAPNTTKRRNISQASQPLMKCRKVFQALIKLAVEELCETADSLIAPVRLGVARPTAPFTLTSSAIEVINGSEELFSIEPLIPHSGVSSQTLDA------------ALQTQQGNNNITIARDVSAMDETEGGEAEGPMDVDGDISEHEESGVSGTVPGQPLGEIDSNAGGVGEEQAGDGESDTELDLLAEAETESDSDDNHSNQDAASAQRSVQTGATAGSDGGMGSILLFPEDESGESSQQEDDESEAGETDEQDNEDFQIGDEQLERRSGSSGHLHRNNLAPVSMQWAIRNREMNTRTAGLRVTGGSNLGFIDSASLRRTTATSAVAAA---QEPIIMGTTTSCLARAFGIVIRQIADLLVMMQDYKMQAPSLPRLMEISYQDALNLQMYLEAHLKPTWDWLLTVMDATEAQLRFGVSLTRSADPTHPEHPLNNAPSLSGGNFTGLLNTAALSLTLQSNAGVGEPSRSSREREGSDAHSARREFLSYCLSLMRAHNGEHRDSLPVLDVSALRHVAYVFDALVYYMRSLSEPMSSRGETQKESSNY---------SSWNDQDENDNDEGEEYNSPVPTALETDSVDYPDLLQVPICGSGNSTNSTPKGRKHPFLQRSDSTLSLGCPPFDPFDTVMSEALPLADQPHLLQPDSRREDLFGIPRQ--SALNSG--SNQNRLAGLPTRLGLSARGTDNQNVSSPTSSQVIQGPAFLSSDAKRSSMSAGDSTSAKPAERAKSFSQPNDHTLAAEQIDRAPIIVSTNNQNEQAAGSTKNNKDICKMNRSVIVRAGTVSE---SNANKAGASE-----MMV-----VSNEMEIVSEETDPPTSSHETSTAQETVETNP---IPTIGSNISHNILLGRWRLSLDLFGRVFMEDVGLEAGSVVSELGGFSVKEAKFRRDMEKLRNSQQRDINLLKLERDRTQLLVQTMKELNTQYNLYNRRASNT-PPLAVNRVKVIFKDEPGEGSGVTRSFYTAIAEALLANEKLPNLEAAQVG-SKYTQYNVLQKLK-SRDRDRELRRQNTRSSGKCREPRRALSFEARVFHPSTSVEGSSNSGAGSSSSNSHPLPVSHPSNEHLTMHQQQLGDRLYPKVYALRPALADKITGMLLELLPAQLLMLLASEDALRQKVEEAFELIRSHSQE------------------------------------------------ATREALL-DLDVFSLIARCGANKKKMENSIL----------DDTEDNAPLFYSPGKRGFYTPRQGRASYERLNAFRNVGRLIGLCLLQNELCPIFLNRHVIKYILGRSIRFHDLAFFDSVIYESLRQLVNDSETKDSNS-LFSTLDLNFSIDLCPEEGGGSIELMPNGRDIEVTASNVYDYVRKYAEVRMIKVQEKALRAMREGVFDVLPEGSLDGLTSEDLRLLLNGVGDINVSVLISYTSFNDESGESADRLAKFKRWLWSIVEKMTHSERQDLLYFWTGSPALPASEDGFQPMPTVTLRPADDAHLPTANTCISRLYVPLYSSRHVLRHKLLLAIKTKNFGFV 2802
BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Match: gi|817197563|ref|XP_012274395.1| (E3 ubiquitin-protein ligase hyd isoform X3 [Orussus abietinus]) HSP 1 Score: 1764.59 bits (4569), Expect = 0.000e+0 Identity = 989/1952 (50.67%), Postives = 1263/1952 (64.70%), Query Frame = 0
Query: 220 GDASPPVIEEPSLPSLPWPPEPMEGNSGDEDSLLGLAGSAASISSHKSVLVKERSPAT---ENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKST--SRKTP-----ELDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGD-SLDCLLTALIRRLQVGSDKG---EARQVAKRFVRSVTRVFVVFNIEMAPSQSKKKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSNDSV--SIEELFSVEPMVPRNSLSVQPENSRSMVSSDHHPRVV-RSVSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEVNDD-GPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG---------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRSRSKSSR------TVNGGFIYIDPNALRRTTSSGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTPGRTRTGFQSRAGSLASGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRSGTDAQEDSPF---GMDPTPMVPSAASNFDENENDDEAAGSGTPA-TNMEVDDEDTNQSAAI-------LAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMTNTLAVLPTRLGLSARTTDEAGATSAVQPAEAFNAMSTAPFQATPITGDSNFENVVRQARPSSPTAATCDTASVRSLDTTVTGGNSEEAETDQPQDLS--MGGGGTMSSASSVSEEPVASTSKGSFTSPKKAFMMREAARGASSAPEVLVVP-------TEKPDQG----DVSANVTIETS---AARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFYGRRSRDAARALNFEARPFYLNGVGG----------------------SNDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGSSGSSAAAAGVAGQEHLLPDLDVFNLGNSSGVKRKSLSGSVALGSEPELQEEENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
G+++P IEEP +P+L WP E + SG+EDSLL + AASIS + S T ++ ERR+NAL L+ +CE+ V APHL+ LL +DAQ TP M A+ RAY AAL+L +A Q+V D K M+ PP + PD SPL V+CCNDTCSFTWTGA+HINQDIFECRTCGLT SLCCCTECARVCH+GHDCKLKKTSPTAYCDCWEKCKCR+LI GHQ ARY +LC+L+ DTDL + N RGE+ILLFLVQTVGRQ EQRQ+R + ++ST SRKTP D MP+HDL PPRFSR+AL+ LL DW A++ MIM+G E N ++ DQ QSGT LLDKFTH LLV+CSG+ L L+ LIR L+ + G EA VA+RFVRSV R+FV+F IEM P+ +K+++ SQPL +CRRVF AL +A+EELCETA++L+APVR G++RPTAPF+L+ S V + EELFS+EP++P + ++ Q ++ ++ V+ RSVS ++ + H +E+ + GEV+ + G V D SD +LDLLAE ++ESD D +++DA S ++ G LF +D+SGES+ ED ES+AGETDEQD+ DF +EQLERR+ R+NLAP SMQWA+R+R ++R T ++IDP++LRR+T++ AT E +TM TTAS LARAFGI IR+IADLLTM QDY +L P L+R +EI++Q++ NL+ +E H+KP WDWLM VMD+TEAQLRFG +L+ S D ++P N P + G R+ G ++ RR+FL+Y LSLMRAHN+EH DSLPVLDV++++H+AYVFDAL+YYMR+ ++ PF G A N + ++DE PA T ME D + + T+ KGR+H FFQRSDSTLCLGCPP DPF T M EALPLADQPHLLQP++RREDLFGVP+Q G N L LPTRLGLSAR D + ++ + S A F + R+A S+ + T +S + GN + + D + + + S A + V T + +A + E + APEVLV TE+ D ++SA+ T+ETS +AR +S+G ++ H++LLGRWR +LDLFGRVF++DVGLE GSI+SELGGF VKEAKFRR+MEKLRNS++ D+ + K+ER+R QL+ Q KE N+ N H +R++S+ PPL VN++KVTF++EP EGSGV RSFYTA+++ALL++ KLPNLE AQ S + + +Q+++ SRD D R QN + R+ RAL+F+AR F+ +NDHL+ HQ QLG+RL+P+V ALRP LA KITGMLLELSPAQLL+LLASE+SLRQ+VEEA ELI SH +++ E LL DLDVF+L G +K + + ++ ++ DNAPLFY+PGK GFY+P QGR + ER+NAFRNVGRL+GLCLLQNELCP+F NRHVIKY+LGR IRFHDLAFFD VIYESLRQLV+DAE KD+++ LFSAL+LTFSIDL EEGGGS EL+PNG DIEV A NVY+YVRKY++ RM QEKALE M+ G+FDVLP G+LD LT+ED RLLLNGVGDINV LISYTSFNDESGE+ ERLV FKRWLW IVEKM+ +E+QDLVYFWTGSPALPASEDGFQPMP+VTIRPA D HLP+ANTCISRLYVPLYSS+ IL+ KLL+AIKTK FGFV
Sbjct: 956 GNSAP--IEEP-IPALSWPSEAFDNTSGEEDSLLSIG--AASISMMTKTGLGSASNNTYIIDSVERRNNALLILRYMCENTVLAPHLKELLTAKDAQGQTPLMLAVSARAYHAALILLDAIQRVGRDA--------KECSLMILPPDANPDLSPLFVLCCNDTCSFTWTGADHINQDIFECRTCGLTGSLCCCTECARVCHRGHDCKLKKTSPTAYCDCWEKCKCRALIAGHQTARYNLLCRLVIDTDLATKINSRGESILLFLVQTVGRQSVEQRQFRSAPRQRSTPASRKTPSADGTSADSYMPDHDLMPPRFSRQALERLLNDWPAVQCMIMSGVSE-----NTNEQLFGDQGQSCRQSGTALLDKFTHSLLVKCSGELYLSTLIVTLIRELRNDTIPGRQEEAINVARRFVRSVARIFVIFTIEMLPNTTKRRNASQPSQPLMKCRRVFHALPKLAVEELCETADSLIAPVRLGVARPTAPFTLTGSAVEVIHNSEELFSIEPLIPHSGVNPQTLDAALQTQQGNNNIVIARSVSGIEEAEAGEEVPMDLDGDISEH------EESGVPSAVAGQPLGEVDGNAGGVGEEQAGDGESDTELDLLAEAETESDSDDNHSNQDAASAQRSVQTGATAGSDGGMGSILLFPEDESGESSQQEDDESEAGETDEQDNDDFQM-DEQLERRSGTSGHLHRNNLAPVSMQWAIRNRELNTRTAGLRVTTGSNLVFIDPSSLRRSTATSAVAAT---QEPITMGTTASCLARAFGIAIREIADLLTMTQDYKTLTPVLSRMMEITFQDAANLETFLEGHLKPTWDWLMTVMDATEAQLRFGVSLTRSADPTHPEHPLNNAPLFSGVGEPPRSSRDREGGES---RREFLSYCLSLMRAHNSEHRDSLPVLDVSALRHVAYVFDALVYYMRTLSE-----PFSSRGESQKVSSTYAPWNDQDENDNDEGEEENLPASTAMETYSADYPDLLQVPICGTGNNSNTSGKGRKHPFFQRSDSTLCLGCPPLDPFDTAMSEALPLADQPHLLQPHSRREDLFGVPRQSTGANQ-------NPLEGLPTRLGLSARGVDNQNPSVIPSFSQVMHGSSYA------------FASESRRANDSNEPGTSKYTEKPKSFNP----GNDNQPMVTEQADRAPIIVSPNSQSDAGCTKNKDVCKTGRSVIV---RAGTVSEPNVNKAGAPEVLVASAMETQNVTEEVDPAATSHEISAHETVETSPVESARAASSIGSNISHNLLLGRWRLSLDLFGRVFMEDVGLEAGSIVSELGGFTVKEAKFRRDMEKLRNSQSKDIALLKVERDRTQLLVQTMKELNTQYNLHNKRAASNTPPLAVNKIKVTFKDEPAEGSGVTRSFYTAIAKALLANEKLPNLESAQVVSRYTQYNIQLQKIK-SRDRDRDLRRQNPRSSGKCRETRRALSFQARSFHPTTSTEGTSGSAPGSSSSNAHPHLVSHPNNDHLTVHQQQLGDRLYPKVHALRPSLAEKITGMLLELSPAQLLMLLASEDSLRQKVEEAFELIHSHVQDLT------------------------------------------------SEALL-DLDVFSLAERCGANKKKMENASSI--------LDDFADNAPLFYSPGKSGFYTPRQGRASFERLNAFRNVGRLIGLCLLQNELCPIFLNRHVIKYILGRPIRFHDLAFFDSVIYESLRQLVIDAETKDSNS-LFSALDLTFSIDLCPEEGGGSIELLPNGGDIEVTATNVYDYVRKYAEVRMIKVQEKALEAMREGVFDVLPEGALDGLTSEDLRLLLNGVGDINVSVLISYTSFNDESGESTERLVKFKRWLWSIVEKMTHVERQDLVYFWTGSPALPASEDGFQPMPNVTIRPA-DDHLPTANTCISRLYVPLYSSRHILRHKLLLAIKTKYFGFV 2785
BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Match: gi|970888464|ref|XP_015109392.1| (PREDICTED: E3 ubiquitin-protein ligase UBR5 isoform X3 [Diachasma alloeum]) HSP 1 Score: 1761.12 bits (4560), Expect = 0.000e+0 Identity = 1009/2031 (49.68%), Postives = 1285/2031 (63.27%), Query Frame = 0
Query: 220 GDASPPVIEEPSLPSLPWPPEPMEGNSGDEDSLLGLAGSAASISSHKSVLVKERSPA---------------------TENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSGPDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYR--PSRSRKSTSRKTPE------LDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLAS--QSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQVGSDKG---EARQVAKRFVRSVTRVFVVFNIEMAPSQSK-KKSLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSNDSV--SIEELFSVEPMVPRNSLSVQPE------NSRSMVSSDHHPRVVRSVSQSNSQALNVSASSAMNDTLELHDQEDNEDE---NQI--GEDAEDGLDGEVNDDGPVENPDQDDQHSDMDLDLLAETDSESD-DGEPNDRDAKSG---------GDNNEAAGGEALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERRATPGTTQERSNLAPQSMQWALRSRSKSSRT----VNGG--FIYIDPNALRRTTSSGTAVATPNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNN---------------------PTPGRTR--TGFQS-----RAGSLASGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRSGTDAQEDSPFGMDPTPMVPSAASNFDENENDDEAAGSGTPATN--MEVDDED--------TNQSAAILAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSGPASMTNTLAVLPTRLGLSARTTDEAGATSAV-QPAEAFNAMSTAPFQATPITGDSNFENVVRQARPSSPTAATCDTASVRSLDTTVTGGNSEEAETDQPQDLSMGGGGTMSSASSVSEEPVASTSKGSFTSPKKAFMMREAARGASSAPEVLVVPT-----EKPDQGD-------VSANVTIETS-------AARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQSGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFYGRRSRDAARALNFEARPFYLNGV------------------------------GGS--------NDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGSSGSSAAAAGVAGQEHLLPDLDVFNLGN--SSGVKRKSLSGSVALGSEPELQEEENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
G+++P IEEP +P L WPPE ++ SG+EDSLL + AASIS V P T++ ERR+NAL LK +CES V APH++ LL +DAQ TP M A+ RAY AAL++ + Q+ S DP K SM+ P + PD SPL V CCNDTCSFTWTGA+HINQDIFECRTCGLT SLCCCTECARVCH+GHDCKLKKTSPTAYCDCWEKCKC++LI GHQGARY++LC+L+ TDL + N RGE+ILLFLVQTVGRQ EQRQ+R P + S SRKTP D +MP+HDL+PPRFSRRAL LL DW A++ MIM+G + + ++ D AF ++ QSGT LLDKFTH LLV+ + + LD LLT LIR L+ S G EA VA+RFVRSV R+FV+F IEMAP+ +K ++S + PL +CR VF+ALI +A+EELCETA++L+APVR G++RPTAPF+L+ S V EELFS+EP++P ++ S+ P + + +++ R V + + S + ++L D + +E E NQ+ GE +G+ G V G + + SD +LDLLAE ++ESD D +++DA S ++ G LF +D+SGES+ ED ES+AGETDEQD +F +EQLERR+ R+NLAP SMQWA+R+R + RT V+GG I+IDP++LRR+T++ A E +TM TTAS LARAFGIVIR IADLLTM+ +Y AP L R +E++YQ++ +LQ+ +E H+KP WDWLM VMD+TEAQLRFG +L+ S D ++P N T G TR GF R G D RR+FL+Y LSLMRAHN EH DSLPVLDV++++H+AYVFDAL+YYMRS + S V S ++ D++END+E T N ME D D T S + + +GR+H F QRSDSTLCLGCPP DPF + EALPLADQPHLLQP++RREDLFGVP+Q + G G + N LPTRLGLS RT D + V Q + +A +++ P ++ RQ PS L+ N E P +S G M+S + V S K + ++ ++R A + APEVLVVP + D+ D +SA+ T+ETS +A V+++G ++ H+ILLGRWR +LDLFGRVF++DVGLE GS++SELGGFPVKEAKFRR+MEKLRNS+ D+ ++KIER+R QL+ Q KE N+ N + RR+S++ PPL VNRVKVTF++EPGEGSGVARSFYTA++ ALL++ KLPNLE AQ GS S + ++Q+L+ RD R QN + R+ RAL+FEAR F+ + GGS N+HL+ HQ LG+RL+P+V ALRP + KITGMLLEL+PAQLL+LLASEE+L+Q+V+EA EL+ +H +V+ E LL DLDVF+L +S K+K + ++ ++VEDNAPLFY PGKRGFY+P QGR + ER+NAFRNVGRL+GLCLLQNELCP+F NRHVIKY+L R IRFHDLAFFD VIYESLRQLV+DAE KD+++ LFS L+LTFSIDL EEGGGS ELVPNGRD+EV A NVY+YVR+Y++ RM QEKALE M+ G+FDVLP G+LD LT+EDFRLLLNGVGDINV LISYTSFNDESGE+ ERLV FKRWLW IVEKM+ E+QDLVYFWTGSPALPASEDGFQPMPSVTIRPA D+HLP+ANTCISRLYVPLYSS+ +L+ K+L+AIKTKNFGFV
Sbjct: 1051 GNSTP--IEEP-IPPLSWPPE-LDNTSGEEDSLLSIG--AASISMMNKATVGNTGPTGSSPNNTNNPNNANNTNNSYITDSTERRNNALLILKYLCESPVLAPHIKELLTGKDAQGQTPLMLAVSVRAYHAALIILDTIQRA----SKDP----KECSSMILPADASPDLSPLFVTCCNDTCSFTWTGADHINQDIFECRTCGLTGSLCCCTECARVCHRGHDCKLKKTSPTAYCDCWEKCKCKALIAGHQGARYDLLCRLVTSTDLATKINSRGESILLFLVQTVGRQSVEQRQFRSGPRQRSTSASRKTPSSDGLGGADSEMPDHDLEPPRFSRRALVRLLNDWPAVQCMIMSGVSD----NSQQDQLFGDGAFSSASRQSGTALLDKFTHTLLVKSTSEMLDTLLTTLIRELENDSIPGRQEEANIVARRFVRSVARIFVIFTIEMAPNTTKNRRSATQATPPLIKCRTVFRALIKLAVEELCETADSLIAPVRLGVARPTAPFTLTSSAIEVINGSEELFSIEPLIPHSAQSLDPTLDAAHVQANVVANNNVGDRAVMDEGSDGPSGVVIDGSRDGGEPMDL-DGDISEHEVPPNQVTLGEVDRNGI-GRVERVGGIAGSE-----SDTELDLLAEAETESDSDDNHSNQDAASAQRSVQTGATAGSDGGMGSILLFPEDESGESSQQEDDESEAGETDEQDTEEFPIGDEQLERRSGSSAHLHRNNLAPVSMQWAIRNRESNPRTAGLRVSGGSNMIFIDPSSLRRSTATSAVAAA---QEPITMGTTASCLARAFGIVIRMIADLLTMISNYKMNAPALPRLMEVTYQDAASLQMYLEGHLKPTWDWLMVVMDATEAQLRFGVSLTQSADPTHPEHPLNNASSVSGRNQRSGITSASNITTTQGSTRLSVGFTGVGEAPRNTREREGGDAHWARREFLSYCLSLMRAHNGEHRDSLPVLDVSALRHVAYVFDALVYYMRSLNEPLGTSRCDNQKENAVHSYSTWNDQDENDNEEGDEDTLHGNNAMETDSVDYPDLLQVPTCGSGNSINSGTGRGRKHPFLQRSDSTLCLGCPPLDPFEANISEALPLADQPHLLQPHSRREDLFGVPRQALAG---GSGTSQNPFEGLPTRLGLSTRTADNSATFGQVMQQGPSASATASSSSSEIPRRSPTSEPGCSRQVPPSDKQKL------YYPLNDNHMMMN--EQIDRAPIIVSPGNQTDMAS------KQVKSDCK-DLSKTGRSVIVRAGAAAETKAPEVLVVPVTETQADNADEVDPATTSQEISAHETVETSPLESTRLSANPVSTIGSNISHNILLGRWRLSLDLFGRVFMEDVGLEAGSVVSELGGFPVKEAKFRRDMEKLRNSQQKDIALSKIERDRTQLLVQTMKELNTQYNLYNRRASNT-PPLAVNRVKVTFKDEPGEGSGVARSFYTAIANALLANDKLPNLEAAQVGSKYS-QYNVLQKLKRDRDRD--FRRQNPRASAKYRETRRALSFEARSFHPSTTLADGASGSGGSSTSASSTGGSSSSNHTLPAGGSHGNHNLSNNEHLTTHQQNLGDRLYPKVHALRPAFSEKITGMLLELTPAQLLMLLASEEALQQKVDEAYELLHNHIQDVT------------------------------------------------SEALL-DLDVFSLTERCASNNKKKIVDNTIL----------DDVEDNAPLFYAPGKRGFYTPRQGRASYERLNAFRNVGRLIGLCLLQNELCPIFLNRHVIKYILSRPIRFHDLAFFDSVIYESLRQLVIDAETKDSNS-LFSTLDLTFSIDLCPEEGGGSIELVPNGRDVEVTATNVYDYVRRYAEVRMIKAQEKALEAMREGVFDVLPEGALDGLTSEDFRLLLNGVGDINVSVLISYTSFNDESGESMERLVKFKRWLWSIVEKMTQFERQDLVYFWTGSPALPASEDGFQPMPSVTIRPADDAHLPTANTCISRLYVPLYSSRHVLRHKILLAIKTKNFGFV 2971
BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Match: gi|1022775425|gb|KZS19284.1| (E3 ubiquitin-protein ligase hyd [Daphnia magna]) HSP 1 Score: 1758.42 bits (4553), Expect = 0.000e+0 Identity = 1060/2174 (48.76%), Postives = 1321/2174 (60.76%), Query Frame = 0
Query: 82 GIKRTLAELESDPTSPNTIETVQRILEERCDGGRNIFHTAVSMCQPTSNKDPDVQDQSAASMSSSSGLDSMDAMSAAY--ASKAMNLRDMMRRATRLELPSSVGGSQAGPSASTSA-PLAPSVAAAAAAAAAVASGGVAPPGDASPPVIEEPSLPSLPWPPEPM---EGNSGDEDSLLGLAGSAASISSHKSVLVKERSPATENQERRSNALASLKLICESLVFAPHLEALLAMQDAQSNTPFMAAIVCRAYPAALVLFEAAQKVALDCSNDPETQKKTLMSMVFPPGSG---PDNSPLHVICCNDTCSFTWTGAEHINQDIFECRTCGLTDSLCCCTECARVCHKGHDCKLKKTSPTAYCDCWEKCKCRSLIGGHQGARYEVLCKLIADTDLVQQANGRGENILLFLVQTVGRQLHEQRQYRPSRSRKS---TSRK--TPELDQDMPEHDLDPPRFSRRALDHLLKDWNALKAMIMTGHKEPNGTGGRNTPMYEDQAFLASQSGTGLLDKFTHCLLVRCSGDSLDCLLTALIRRLQ---VGSDKGEARQVAKRFVRSVTRVFVVFNIEMAPSQSKKK----SLQAISQPLHRCRRVFQALINIAIEELCETANALLAPVRFGISRPTAPFSLSPSN-DSV-SIEELFSVEPMVPRNSLSVQPENSR-------SMVSSDHHPRVVRS-VSQSNSQALNVSASSAMNDTLELHDQEDNEDENQIGEDAEDGLDGEVNDDGPVENPDQDDQHSDMDLDLLAETDSESDDGE---PNDRDAKSGGDNNEA---------AGGE------ALFSDDDSGESTHPEDGESDAGETDEQDDADFTFSEEQLERR------ATPGT----TQERSNLAPQSMQWALRSRSKSSRTVNGGFIYIDPNALRRTTSSGTAVAT-----------------------------PNGSEGLTMYTTASSLARAFGIVIRQIADLLTMLQDYNSLAPGLARTLEISYQESINLQLLIEYHMKPNWDWLMAVMDSTEAQLRFGSALSYSTDLAPAQNPNNPTP--GRTRTGFQSRAGSL------------------------------------------ASGQDTAHNRRDFLNYALSLMRAHNAEHSDSLPVLDVASMKHIAYVFDALIYYMRSGTDAQEDSPFGMDPTPMVPSAASNFDENE----NDDEAAGSGTPATNMEVDDEDTNQSAAILAATASKGRRHAFFQRSDSTLCLGCPPPDPFHTPMQEALPLADQPHLLQPNARREDLFGVPKQPMGGEDSG--PASMTNTLAVLPTRLGLSART----TDEAGATSAVQPAEAFNAMS--TAPFQATPITGDSNFENV---------VRQARPSSPTAATCDTASVRSLDTTVTGGNSEEAETDQPQDLSMGGGGTMSSASSVSEEPVASTSKGSFTSPKKAFMMREAAR----GASSAPEVLVVPTEKPDQGDVSANVTIETSAARRVNSLGVSVPHDILLGRWRSALDLFGRVFVDDVGLEPGSIISELGGFPVKEAKFRREMEKLRNSRTVDLTINKIERERDQLITQAFKEFNSHVNQHMRRSSSSQPPLVVNRVKVTFQNEPGEGSGVARSFYTALSEALLSSGKLPNLEGAQ--SGSSKSMHFGLIQRLRGSRDAQIDRRIQNRFYG----RRSRDA-ARALNFEARPFYLNG--VGGSNDHLSHHQTQLGERLFPRVQALRPILANKITGMLLELSPAQLLLLLASEESLRQRVEEAVELIASHGGNVSEPGSSNASGVMASAAGTGGQPSGAGPNPLLGGGAGSSGSSAAAAGVAGQEHLLPDLD-VFNLGNSSGVKRKSLSGSVALGSEPELQEEENVEDNAPLFYTPGKRGFYSPIQGRGTPERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSHLPSANTCISRLYVPLYSSKAILKSKLLMAIKTKNFGFV 2088
G++ L LE+ + + VQ I+ ERCDGGRN+ H VSMC PTSNKD DVQ+ A + + D +D + +S+A++LR+MMRRA + A PS +++A P AP ++ A E S+P+L WPPEP+ N D+D + A S S +V ++ ERR+N+LA+LK I ES HL+ LL+ +DAQ +TPFM A+ RAYPAALVL + A KV+ + + ETQKK L S +FP G PD+SPLHV+C NDTCSFTWTGAEHINQDIFECRTCGLT SLCCCTECARVCHKGHDCKLK+TSPTAYCDCWEKCKC++LI G+Q R+E+LC+LI++TDLV N +GENILLFLVQTVGRQ EQRQYRP+RSR + SRK T EL+ DMPEHDL+PPRF+R ALD LL DW+A+KAM++ G+KE TG P EDQ +L SQSGT LLDKFTHC+LV+CS + LD LLT LI ++ + + EAR A+RFVRSV RVFVVF++E+AP KKK S SQPL +C+RVFQALIN+AIEELCE A+AL+ PVR G++RPT PF L SN D++ EELFSV+P+ P + E++ S+ ++ R+ + S+ L+ + + + DN D EDAE +G D + SDM+LDLLAET+S+SDD E ND + DNN AG + ALFSDD+SG+ST E+ ESD GETDEQD +F FS+EQLERR P T + S +APQSM WA+R R ++ V G P A TSSG AV + P E +M TTAS LARAFG+V+RQIADLLTML DY +LAP L+R L+I+ ESI LQ +E+ +KP WDWL+ VMDSTEAQLRFG ALS ++ P P+N GRT A S+ + D RR+FLNY LSLMRAHN+EHSD+LPV+DV+S+KHIAYVFDALIYYMRSG +A +P+G D A F+E + + E G+G V E+ GR+H FF+RS+STLCLGCP PDPF PM EALPLADQPHLLQP+ARRE+LFG P+QP+ + SG P +N L V+PTRLGLS RT TD+ A A N+ P Q+TP +N+ V + +SP+ A+ S R TV S T +D SVS P ASTS + + +K AA G S+AP V + + D+LLGRWR LDLFGRVF+DDVG E GS+I+ELGGFPVKEA+FRREME+LRN + DLT+ K++RER LITQ FKE N+ N H RR++++QPPL V+RVKVTF++EPGEGSGVARSFYTA++EA+LS KLPNL+ AQ +GS S + R R D Q+R +G + RDA R LN++ARPF +G +NDHLS H QLGERL+PRV ALRP LA KITGMLLELSPAQLL+LLASE+SL QRV EAV +I G S A G + ++D +FNLGN +++ + GS+ + EE +D APLFY PGK+GFYSP QG+ TPER+NAFRNVGRL+GLCLLQNELCP+ NRHV+K +LGR IRFHDLAFFDPV+YES RQLV+DAE KD S L+LTFSIDLS +EG G EL+P G+D+EV + N+Y+YVR+Y+QYRM Q KA+E+++LG+FDVLP +L+ LT+ED RLLLNG G+I VQTLISYTSFNDESGE PERL+ FKRWLW IVEKM+ E+QDLVYFWTGSPALPASE+GFQPMP+VTIRPA D HLP+ANTCISRLYVPLYSS+ IL++KLL+AI+TKNFGFV
Sbjct: 904 GVRHVLNCLETS-SEAYRLSGVQSIVNERCDGGRNVIHACVSMCSPTSNKDADVQEDRNAPPAVPAIADPLDPLPVGLSSSSRAVSLREMMRRA-----------AVARPSENSAADPGAPGSSSVAE---------------------ENISIPTLNWPPEPLSLESSNHADDDQAGSSSAGACGSSKMASAVV------SDPVERRANSLAALKAITESPALNFHLQTLLSARDAQGHTPFMQAVAVRAYPAALVLLDVATKVSRQLATEVETQKKLLQSFIFPLGKNGCSPDDSPLHVLCSNDTCSFTWTGAEHINQDIFECRTCGLTGSLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKALIAGNQKLRFELLCRLISETDLVNIPNVKGENILLFLVQTVGRQSAEQRQYRPARSRSTGIAASRKVLTSELEPDMPEHDLEPPRFARMALDRLLGDWSAVKAMLLLGYKEETSTGSTTIP--EDQLYLQSQSGTTLLDKFTHCMLVKCSVEMLDSLLTTLIAEMENKTWWTRQQEARLAARRFVRSVVRVFVVFSVEIAPQSGKKKNSASSASVTSQPLMKCKRVFQALINLAIEELCEAADALIVPVRLGVARPTVPFPLVTSNADAIHGSEELFSVDPLQPLTPRAADEEHADGSSLPAGSLGATSGTTGGGRANTRRMESEELDAIEGDEGDGEGDGEGEADNSDH----EDAEG--NGRSGDGTGRRDGANAGGESDMELDLLAETESDSDDNEHEHDNDNQSTHTQDNNSVQRSVQTGATAGSDAAMASLALFSDDESGDSTGQEEDESDGGETDEQDTEEFAFSDEQLERRNNNSSSTVPATAAAPVRGTSGMAPQSMMWAIRHRENNA--VRG------PGANGAVTSSGNAVGSSTTNLMYLEPTSLRRSAAAAALAAASATIPVVPEPFSMATTASGLARAFGLVVRQIADLLTMLLDYPALAPALSRNLDITNHESIQLQQFLEFRLKPVWDWLVNVMDSTEAQLRFGVALSNAS--TPNGTPSNHITLYGRTPLSRMVTASSVLPSARTAPSSSSAASALAGSAAGAGTYDGRRGQTGGTGGERSSEVPDPLTGRREFLNYCLSLMRAHNSEHSDTLPVIDVSSLKHIAYVFDALIYYMRSGNEA---NPYG-DNGSGEDYIADMFNEPDENENAEMEEEGTGGAMDTESVGGEE--------EPVVRNGRKHPFFRRSESTLCLGCPQPDPFALPMVEALPLADQPHLLQPSARREELFGAPRQPITIQPSGNHPPGSSNPLEVIPTRLGLSTRTPEIQTDQPTAPLPPLVATPINSQQEPLPPNQSTPEVIQETPQNLSLKDSESIEVIEVTRASPSVASSVIVSPRRAPATV---QSVIVRTGNVRD-------------SVSSGPSASTSAPTSATREKTEETVPAASSFWLGKSTAPNVCQL------------------------------ISSDLLLGRWRLTLDLFGRVFIDDVGTEAGSVIAELGGFPVKEARFRREMERLRNMQQRDLTLFKMDRERGMLITQTFKELNTQYNTHCRRATATQPPLAVSRVKVTFKDEPGEGSGVARSFYTAIAEAILSQEKLPNLDAAQVGAGSKASQSQSGMARRRNRSSISGD---QSRRHGPPRLAQVRDARLRTLNYDARPFQPDGDVPTTNNDHLSPHVIQLGERLYPRVAALRPTLAAKITGMLLELSPAQLLMLLASEDSLEQRVNEAVAVIL--------------------------------------------GQSQLAQGRELSSEAMMEMDLLFNLGNRK--NPTTVTAGTSTGSDADEGVEE--DDTAPLFYCPGKQGFYSPRQGKATPERLNAFRNVGRLMGLCLLQNELCPIALNRHVLKAILGRPIRFHDLAFFDPVVYESFRQLVLDAETKD-----LSGLDLTFSIDLSPDEGSGFLELIPGGKDVEVTSQNIYSYVRRYAQYRMVRTQSKAMESIRLGIFDVLPQNALEGLTSEDLRLLLNGTGEIRVQTLISYTSFNDESGEPPERLLRFKRWLWSIVEKMTTQERQDLVYFWTGSPALPASEEGFQPMPTVTIRPADDVHLPTANTCISRLYVPLYSSRTILRNKLLLAIRTKNFGFV 2906 The following BLAST results are available for this feature:
BLAST of e3 ubiquitin-protein ligase hyd vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 12
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BLAST of e3 ubiquitin-protein ligase hyd vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of e3 ubiquitin-protein ligase hyd vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold114_size351134:135644..156841+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold114_size351134-snap-gene-1.12 ID=maker-scaffold114_size351134-snap-gene-1.12|Name=e3 ubiquitin-protein ligase hyd|organism=Tigriopus kingsejongensis|type=gene|length=21198bp|location=Sequence derived from alignment at scaffold114_size351134:135644..156841+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'e3 ubiquitin-protein ligase hyd' has the following synonyms
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