EMLSAG00000006171, EMLSAG00000006171-688937 (gene) Lepeophtheirus salmonis

Overview
NameEMLSAG00000006171
Unique NameEMLSAG00000006171-688937
Typegene
OrganismLepeophtheirus salmonis (salmon louse)
Associated RNAi Experiments

Nothing found

Homology
BLAST of EMLSAG00000006171 vs. GO
Match: - (symbol:lack "lethal with a checkpoint kinase" species:7227 "Drosophila melanogaster" [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISS;IBA;NAS] [GO:0007179 "transforming growth factor beta receptor signaling pathway" evidence=IGI] [GO:0005515 "protein binding" evidence=IPI] [GO:0008101 "decapentaplegic signaling pathway" evidence=IMP] [GO:0048619 "embryonic hindgut morphogenesis" evidence=IMP] [GO:0001700 "embryonic development via the syncytial blastoderm" evidence=IMP] [GO:0042787 "protein ubiquitination involved in ubiquitin-dependent protein catabolic process" evidence=IMP] [GO:0040008 "regulation of growth" evidence=IGI;IMP] [GO:0010629 "negative regulation of gene expression" evidence=IDA] [GO:0007293 "germarium-derived egg chamber formation" evidence=IGI] [GO:0030514 "negative regulation of BMP signaling pathway" evidence=IGI] [GO:0005634 "nucleus" evidence=IBA] [GO:0005737 "cytoplasm" evidence=IBA] Pfam:PF00632 InterPro:IPR000008 InterPro:IPR000569 InterPro:IPR001202 Pfam:PF00168 Pfam:PF00397 PROSITE:PS01159 PROSITE:PS50004 PROSITE:PS50020 PROSITE:PS50237 SMART:SM00119 SMART:SM00239 SMART:SM00456 UniPathway:UPA00143 EMBL:AE013599 GO:GO:0005634 GO:GO:0005737 GO:GO:0001700 SUPFAM:SSF49562 GO:GO:0040008 GO:GO:0010629 GO:GO:0004842 SUPFAM:SSF51045 eggNOG:COG5021 GO:GO:0042787 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 GO:GO:0000151 GO:GO:0030514 GO:GO:0048619 GO:GO:0007293 GO:GO:0008101 EMBL:AF416571 EMBL:AF464851 EMBL:AF216521 EMBL:BT021410 RefSeq:NP_001261061.1 RefSeq:NP_523779.1 UniGene:Dm.2783 ProteinModelPortal:Q9V853 SMR:Q9V853 BioGrid:62687 IntAct:Q9V853 MINT:MINT-4080844 PaxDb:Q9V853 EnsemblMetazoa:FBtr0086833 EnsemblMetazoa:FBtr0333168 GeneID:36999 KEGG:dme:Dmel_CG4943 CTD:36999 FlyBase:FBgn0029006 InParanoid:Q9V853 KO:K04678 OMA:NQAARPF OrthoDB:EOG77Q4W4 PhylomeDB:Q9V853 SignaLink:Q9V853 ChiTaRS:lack GenomeRNAi:36999 NextBio:801434 Bgee:Q9V853 Uniprot:Q9V853)

HSP 1 Score: 597.43 bits (1539), Expect = 0.000e+0
Identity = 274/392 (69.90%), Postives = 326/392 (83.16%), Query Frame = 0
Query:  542 GTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLT-NAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            G + LPK+++DLV K++ LR+ L   QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKF+ EEGLDYGGVAREWL+LLS EMLNP YGLFQYS +D YTLQINPDS VNPDHLSYFHFVGR +G+AVFHGH +DG FTTPFYK +LNKPI L DIE VDPDLH+SL W+L ++ISGII++TFSVE+ +FG   V+ELK  G  IPV E NK EYVKLYV +R+  GIEQQFL+LQKGF ELIP +LL+PFDE+ELELVIGGI +ID+ DW+N+TRLK C  ET  V WFW +V+SYSSEMRARLLQFVTGSSRVPLQGF+ LQGSTG  GPR FT+HLT + P  +LPKA TCFNR+DLP Y +++++ +KLTQAVEETCGFAVE
Sbjct:  670 GADLLPKYRRDLVGKLRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE 1061          

HSP 2 Score: 211.075 bits (536), Expect = 4.635e-55
Identity = 103/200 (51.50%), Postives = 140/200 (70.00%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVS----DTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNL-------TCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTT 195
            K+R+TILCARNLA++DLFRLPDPFA+V VD TG  + +E +K++ DPKWN HYDL L   ++ T++VWN +K+HK    SGFLGCVR+    IQ +K  G+QRLDL      D ++   V+GQI+ISLLS+DG  +G+  A+V   G++       +  + LPEGWEERRT +GRVYYVNH  ++TQW RP +P   G++
Sbjct:   14 KVRITILCARNLARKDLFRLPDPFAKVQVDGTGQVYSTEISKSSLDPKWNAHYDLFLGIGDAITITVWNQRKIHK---GSGFLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDEL---VRGQIIISLLSKDGPSSGNPLAIVGPSGDVRGPSEDDSSEDSLPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSS 207          

HSP 3 Score: 102.449 bits (254), Expect = 8.918e-21
Identity = 48/81 (59.26%), Postives = 61/81 (75.31%), Query Frame = 0
Query:  283 NLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRAL-TSNEELN-LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            +LPPGY M+TT+ GQ+Y+ H  TG +TWHDPR+ R   T +  L+ +GPLP GWEQR T S R YFV+HNN TTQF+DPR+
Sbjct:  514 DLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIPRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRL 594          

HSP 4 Score: 48.521 bits (114), Expect = 3.660e-4
Identity = 25/54 (46.30%), Postives = 37/54 (68.52%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSS 210
            LP GWE+R+T SGRVY+V+H  RTTQ+   T+P   G+  +  R GT+  T+++
Sbjct:  563 LPSGWEQRKTASGRVYFVDHNNRTTQF---TDPRLSGSILQMIRRGTVPPTSAA 613          
BLAST of EMLSAG00000006171 vs. GO
Match: - (symbol:smurf2 "SMAD specific E3 ubiquitin protein ligase 2" species:7955 "Danio rerio" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0016874 "ligase activity" evidence=IEA] [GO:0005886 "plasma membrane" evidence=IEA] [GO:0005634 "nucleus" evidence=IEA] [GO:0016020 "membrane" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IEA] Pfam:PF00632 InterPro:IPR000008 InterPro:IPR000569 InterPro:IPR001202 InterPro:IPR024928 Pfam:PF00168 Pfam:PF00397 PIRSF:PIRSF001569 PROSITE:PS01159 PROSITE:PS50004 PROSITE:PS50020 PROSITE:PS50237 SMART:SM00119 SMART:SM00239 SMART:SM00456 UniPathway:UPA00143 ZFIN:ZDB-GENE-030131-1830 GO:GO:0005886 GO:GO:0005634 GO:GO:0005737 GO:GO:0045121 SUPFAM:SSF49562 GO:GO:0004842 SUPFAM:SSF51045 eggNOG:COG5021 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 HOVERGEN:HBG004134 HOGENOM:HOG000208451 TreeFam:TF323658 KO:K04678 OrthoDB:EOG77Q4W4 EMBL:BC155849 RefSeq:NP_001107898.1 UniGene:Dr.77630 ProteinModelPortal:A9JRZ0 SMR:A9JRZ0 STRING:7955.ENSDARP00000055490 Ensembl:ENSDART00000055491 GeneID:563633 KEGG:dre:563633 CTD:64750 OMA:PACEYSS NextBio:20884992 PRO:PR:A9JRZ0 Bgee:A9JRZ0 Uniprot:A9JRZ0)

HSP 1 Score: 585.874 bits (1509), Expect = 0.000e+0
Identity = 309/629 (49.13%), Postives = 400/629 (63.59%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDAN-----NHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPS-----TSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSN------------ITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVP----NTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T S R  ++NH   +TQ+  P  P    ++     +   D+N+P     G +        +      S    +  S      P D P      T+ +  +    + +G ++      P  L+  N            I NT    V     NN           + + F    +S++  ++ +P P        + SSS P         +  P   P+DP  + +        PK+K+DLV K+K+LR  L+  QPQ G+C +EVSR+E+FE+SYRQ++KMR K + K+L VKFR EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS +D+YTLQINPDSAVNP+HLSYFHFVGRI+GMAVFHGH+IDG FT PFYK +L KPI LDD+E+VDPDLH SL WIL NDI+G++D+TF VEH A+G    +ELK NGK IPV +  K EYV+LYV +R+  GIE QFL+LQKGFNE+IPQ+LLK FDEKELEL++ G+G IDI DWK++TRLK C  ++ +V+WFW  V+SY  E RARLLQFVTGSSRVPLQGFK LQG+    GPR FT+H  +A  ++LPKA TCFNR+D+P Y S++ + +KL  A+EETCGFAVE
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRSTQWERPTRPASEYSSPGRPLSCLVDENTPIMTPNGAAGVPADDPRVQERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNVNCEELGPLPPGWEIRNTATGRVYFVDHNN-----------RTTQFTDPRLSANLHLVLNPSPNGSRAAVEAQSSSRPGQLKEQAQSVVSPGNLPEDPECLTV--------PKYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILDNDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTQDTKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDINDWKSNTRLKHCTPDSNIVKWFWRAVESYDEERRARLLQFVTGSSRVPLQGFKALQGA---AGPRLFTIHQIDASTNNLPKAHTCFNRIDIPPYESYDKLYDKLLTAIEETCGFAVE 765          

HSP 2 Score: 309.686 bits (792), Expect = 3.879e-90
Identity = 175/360 (48.61%), Postives = 220/360 (61.11%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG----LPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
             K+RLT+LCA+NL K+D FRLPDPFA+V VD +G  H ++  +NT DPKWN HYDL++  ++S T+SVWN KK+HKK   +GFLGCVRL+ N+I R+KDTGYQRLDL    K+G      V+GQIV+SL SRD  G+G    VVD   +    NDLP+GWEERRT SGR+ Y+NH+ R+TQW RPT PAS                 SS    LS      T   TP   GA  +P               P V  +   S     + S   R H  T PD LP GY  +TT+ GQ+Y++H  TG +TWHDPR+ R L++     LGPLPPGWE R T + R YFV+HNN TTQF+DPR+
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQHYDLYIGKSDSITISVWNHKKIHKKQ-GAGFLGCVRLLSNSINRLKDTGYQRLDL---NKLGPNDSDTVRGQIVVSLQSRDRIGSGG--PVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRSTQWERPTRPAS---------------EYSSPGRPLSCLVDENTPIMTPN--GAAGVPADD------------PRVQERRVRSQRHRNYMS---RTHLHTPPD-LPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNVNCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330          
BLAST of EMLSAG00000006171 vs. GO
Match: - (symbol:SMURF1 "Uncharacterized protein" species:9615 "Canis lupus familiaris" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] Pfam:PF00632 InterPro:IPR000569 InterPro:IPR001202 Pfam:PF00397 PROSITE:PS01159 PROSITE:PS50020 PROSITE:PS50237 SMART:SM00119 SMART:SM00456 GO:GO:0016567 GO:GO:0004842 SUPFAM:SSF51045 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 EMBL:AAEX03004280 Ensembl:ENSCAFT00000049516 Uniprot:J9NW19)

HSP 1 Score: 577.015 bits (1486), Expect = 0.000e+0
Identity = 268/406 (66.01%), Postives = 332/406 (81.77%), Query Frame = 0
Query:  530 PQDPLSIALHGG-GTEFLP--KFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            P  PL +   G    E LP  ++++DLV K+KVLR  L+  QPQ G+C +EVSR+E+FE+SYRQI+KMR K ++K+L VKFR EEGLDYGGVAREWLYLL HEMLNPYYGLFQYS++++Y LQINPDS++NPDHLSYFHFVGRI+G+AVFHGH+I+G FT PFYK +L KPI L D+E+VDP+LHKSL WIL NDI+ ++D+TF VEH AFG    +ELK NG+++PV E NK EYV+LYV +R+  GIE QFL+LQKGFNELIPQ+LLKPFD+KELEL+IGG+  ID+ DWK++TRLK C A++ +V+WFW  V+++  E RARLLQFVTGS+RVPLQGFK LQGSTG  GPR FT+HL +A  D+LPKA TCFNR+D+P Y S+E + EKL  AVEETCGFAVE
Sbjct:  151 PSQPLPLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDVSDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE 556          

HSP 2 Score: 105.531 bits (262), Expect = 3.796e-22
Identity = 62/138 (44.93%), Postives = 79/138 (57.25%), Query Frame = 0
Query:  255 VNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTN----PSRGTSKNE 388
            + N +   SL  P    Q R H    P+ LP GY  +TT  GQ+Y++H  TG +TWHDPR+ R L S     LGPLPPGWE R T S R YFV+HNN TTQF+DPR   ++   NH      P+     PS G+ ++E
Sbjct:   37 LRNPEVRGSLQTP----QNRPHGHQSPE-LPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPR---LHHIMNHQCQLKEPSQPLPLPSEGSVEDE 166          
BLAST of EMLSAG00000006171 vs. GO
Match: - (symbol:smurf1 "SMAD specific E3 ubiquitin protein ligase 1" species:7955 "Danio rerio" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA;IBA] [GO:0042787 "protein ubiquitination involved in ubiquitin-dependent protein catabolic process" evidence=IBA] [GO:0007179 "transforming growth factor beta receptor signaling pathway" evidence=IBA] [GO:0005737 "cytoplasm" evidence=IBA] [GO:0005634 "nucleus" evidence=IBA] [GO:0016874 "ligase activity" evidence=IEA] Pfam:PF00632 InterPro:IPR000008 InterPro:IPR000569 InterPro:IPR001202 InterPro:IPR024928 Pfam:PF00168 Pfam:PF00397 PIRSF:PIRSF001569 PROSITE:PS50004 PROSITE:PS50020 PROSITE:PS50237 SMART:SM00119 SMART:SM00239 SMART:SM00456 UniPathway:UPA00143 ZFIN:ZDB-GENE-040426-2744 GO:GO:0005634 GO:GO:0005737 SUPFAM:SSF49562 GO:GO:0007179 GO:GO:0004842 SUPFAM:SSF51045 eggNOG:COG5021 GO:GO:0042787 SUPFAM:SSF56204 HOVERGEN:HBG004134 HOGENOM:HOG000208451 KO:K04678 CTD:57154 EMBL:BC059201 RefSeq:NP_001001943.1 UniGene:Dr.76814 ProteinModelPortal:Q6PCR3 SMR:Q6PCR3 STRING:7955.ENSDARP00000003721 GeneID:321695 KEGG:dre:321695 InParanoid:Q6PCR3 NextBio:20807489 PRO:PR:Q6PCR3 Uniprot:Q6PCR3)

HSP 1 Score: 582.793 bits (1501), Expect = 0.000e+0
Identity = 267/399 (66.92%), Postives = 327/399 (81.95%), Query Frame = 0
Query:  534 LSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            + + +  G  E   ++++DLV K+KVLR  L+  QPQ G+C +EVSR+E+FE+SYRQI+KMR K ++K+L VKFR EEGLDYGGVAREWLYLL HEMLNPYYGLFQYS++++YTLQINPDS++NPDHLSYFHFVGRI+G+AVFHGH+I+G FT PFYK +L KPI L D+E VDP+LHKSL WIL NDI+ ++D+TF VEH AFG    +ELK NGK+IPV E NK EYV+LYV +R+  GIE QFL+LQKGFNELIPQ+LLKPFD KELEL+IGG+G ID+ DWK +TRLK C A++ +V+WFW  V+S+  E R RLLQFVTGS+RVPLQGFK LQGSTG  GPR FT+HL +A  D+LPKA TCFNR+D+P Y S+E + EKL  AVEETCGFAVE
Sbjct:  333 MEVGMEEGDGELPVRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTLPFYKQLLGKPIQLCDLETVDPELHKSLVWILENDITSVLDHTFCVEHNAFGKFLQHELKPNGKNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDNKELELIIGGLGKIDLNDWKANTRLKHCVADSNIVKWFWQAVESFDEERRGRLLQFVTGSTRVPLQGFKALQGSTGSAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE 731          

HSP 2 Score: 184.882 bits (468), Expect = 2.185e-47
Identity = 120/323 (37.15%), Postives = 167/323 (51.70%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLV----SDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEEL 325
            KIRLT+LCA+NLAK+D FRLPDPFA+V VD +G  H ++  K+T DPKWN HYDL++   +S T+SVWN KK+HKK   +GFLGCVRL+ NAI R+KDTGYQRLDL     SD+     V+GQIV+SL +RD  G+G    VVD  G +     + E     R  S        +     +  PT  A  G  R  +         + +  N      T T  + P    +  LP       T  G   +  ++ Q   S+  DP            +   LPPG  +++T  G+IY++      T + DPRL   ++ + ++
Sbjct:   14 KIRLTVLCAKNLAKKDFFRLPDPFAKVVVDGSGQCHSTDTVKSTLDPKWNQHYDLYIGKTDSITISVWNHKKIHKKQ-GAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDSD---AVRGQIVVSLQTRDRIGSGG--PVVDCRGLVENEGPVFEDSGPGRPLSC------FMEEPMPYTDPTGAAGGGNCRALESPNQEQRLQAQRIRNQDSRGHTHTPQNRPHGHQSPDLPEGYEQRTTVQG--QVYFLHTQTGVSTWHDPRIPRDLNSVSCEELGPLPPGREIRSTVSGRIYFVDHNNRTTQFTDPRLHHIMSQHSQV 322          

HSP 3 Score: 103.605 bits (257), Expect = 2.521e-21
Identity = 56/107 (52.34%), Postives = 66/107 (61.68%), Query Frame = 0
Query:  255 VNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            + NQDS      P    Q R H    PD LP GY  +TT  GQ+Y++H  TG +TWHDPR+ R L S     LGPLPPG E R T S R YFV+HNN TTQF+DPR+
Sbjct:  211 IRNQDSRGHTHTP----QNRPHGHQSPD-LPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVSCEELGPLPPGREIRSTVSGRIYFVDHNNRTTQFTDPRL 312          
BLAST of EMLSAG00000006171 vs. GO
Match: - (symbol:Smurf1 "SMAD specific E3 ubiquitin protein ligase 1" species:10090 "Mus musculus" [GO:0000209 "protein polyubiquitination" evidence=ISO] [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISO;ISS;IBA] [GO:0005634 "nucleus" evidence=IBA] [GO:0005737 "cytoplasm" evidence=ISO;IBA] [GO:0005886 "plasma membrane" evidence=ISO] [GO:0006611 "protein export from nucleus" evidence=ISO] [GO:0007179 "transforming growth factor beta receptor signaling pathway" evidence=IBA] [GO:0016020 "membrane" evidence=IEA] [GO:0016567 "protein ubiquitination" evidence=ISO;IMP] [GO:0016874 "ligase activity" evidence=IEA] [GO:0030154 "cell differentiation" evidence=ISO;ISS] [GO:0030279 "negative regulation of ossification" evidence=IMP] [GO:0030424 "axon" evidence=ISO] [GO:0030509 "BMP signaling pathway" evidence=ISO] [GO:0030512 "negative regulation of transforming growth factor beta receptor signaling pathway" evidence=ISO] [GO:0030579 "ubiquitin-dependent SMAD protein catabolic process" evidence=ISO] [GO:0032801 "receptor catabolic process" evidence=ISO] [GO:0034394 "protein localization to cell surface" evidence=ISO] [GO:0042787 "protein ubiquitination involved in ubiquitin-dependent protein catabolic process" evidence=ISO;ISS;IBA] [GO:0043025 "neuronal cell body" evidence=ISO] [GO:0043161 "proteasome-mediated ubiquitin-dependent protein catabolic process" evidence=ISO] [GO:0070062 "extracellular vesicular exosome" evidence=ISO] [GO:0070411 "I-SMAD binding" evidence=ISO] [GO:0070412 "R-SMAD binding" evidence=ISO] Pfam:PF00632 InterPro:IPR000008 InterPro:IPR000569 InterPro:IPR001202 InterPro:IPR024928 Pfam:PF00168 Pfam:PF00397 PIRSF:PIRSF001569 PROSITE:PS01159 PROSITE:PS50004 PROSITE:PS50020 PROSITE:PS50237 SMART:SM00119 SMART:SM00239 SMART:SM00456 UniPathway:UPA00143 MGI:MGI:1923038 GO:GO:0005886 GO:GO:0005634 GO:GO:0005737 GO:GO:0030154 GO:GO:0043161 GO:GO:0043025 SUPFAM:SSF49562 GO:GO:0030509 GO:GO:0007179 GO:GO:0031398 GO:GO:0030279 GO:GO:0004842 GO:GO:0000209 GO:GO:0034394 SUPFAM:SSF51045 eggNOG:COG5021 GO:GO:0042787 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 GO:GO:0030512 GO:GO:0006611 GO:GO:0032801 GO:GO:0030579 HOVERGEN:HBG004134 HOGENOM:HOG000208451 TreeFam:TF323658 KO:K04678 CTD:57154 EMBL:AK015264 EMBL:AK154491 EMBL:BC029097 RefSeq:NP_001033716.1 RefSeq:NP_083714.3 UniGene:Mm.27735 ProteinModelPortal:Q9CUN6 SMR:Q9CUN6 BioGrid:217743 IntAct:Q9CUN6 MINT:MINT-1340776 STRING:10090.ENSMUSP00000106305 PhosphoSite:Q9CUN6 PRIDE:Q9CUN6 Ensembl:ENSMUST00000085684 GeneID:75788 KEGG:mmu:75788 UCSC:uc009alv.1 NextBio:343970 PRO:PR:Q9CUN6 ArrayExpress:Q9CUN6 Bgee:Q9CUN6 CleanEx:MM_SMURF1 Genevestigator:Q9CUN6 Uniprot:Q9CUN6)

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 269/406 (66.26%), Postives = 333/406 (82.02%), Query Frame = 0
Query:  530 PQDPLSIALHGG-GTEFLP--KFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            P  PL +   G    E LP  ++++DLV K+KVLR  L+  QPQ G+C +EVSR+E+FE+SYRQI+KMR K ++K+L VKFR EEGLDYGGVAREWLYLL HEMLNPYYGLFQYS++++YTLQINPDS++NPDHLSYFHFVGRI+G+AVFHGH+I+G FT PFYK +L KPI L D+E+VDP+LHKSL WIL NDI+ ++D+TF VEH AFG    +ELK NG+++PV E NK EYV+LYV +R+  GIE QFL+LQKGFNELIPQ+LLKPFD+KELEL+IGG+  ID+ DWK++TRLK C A++ +V+WFW  V+++  E RARLLQFVTGS+RVPLQGFK LQGSTG  GPR FT+HL +A  D+LPKA TCFNR+D+P Y S+E + EKL  AVEETCGFAVE
Sbjct:  326 PSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE 731          

HSP 2 Score: 177.178 bits (448), Expect = 1.016e-44
Identity = 123/326 (37.73%), Postives = 164/326 (50.31%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLV----SDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNL---PPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEEL 325
            KIRLT+LCA+NLAK+D FRLPDPFA++ VD +G  H ++  KNT DPKWN HYDL++   +S T+SVWN KK+HKK   +GFLGCVRL+ NAI R+KDTGYQRLDL     SDT     V+GQIV+SL +RD  G G   +VVD  G L     + E     R  S           T          +C       +   L +    +  N        T  + P    +  LP       T  G   +  ++ Q   S+  DP     PR       D L   PPG+ +++T  G+IY++      T + DPRL   +    +L
Sbjct:   14 KIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQ-GAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTD---AVRGQIVVSLQTRDRIGGGG--SVVDCRGLLENEGTVYEDSGPGRPLS--CLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLV---QRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQG--QVYFLHTQTGVSTWHDPRI---PRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQL 323          

HSP 3 Score: 108.612 bits (270), Expect = 7.580e-23
Identity = 59/131 (45.04%), Postives = 74/131 (56.49%), Query Frame = 0
Query:  267 PFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTN 397
            P  + Q R H    P+ LP GY  +TT  GQ+Y++H  TG +TWHDPR+ R L S     LGPLPPGWE R T S R YFV+HNN TTQF+DPR   ++   NH      P+ P +  S+       LP  
Sbjct:  220 PLQTPQNRPHGHQSPE-LPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPR---LHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQ 346          
BLAST of EMLSAG00000006171 vs. GO
Match: - (symbol:SMURF1 "E3 ubiquitin-protein ligase" species:9031 "Gallus gallus" [GO:0000209 "protein polyubiquitination" evidence=IEA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] [GO:0005737 "cytoplasm" evidence=IEA] [GO:0005886 "plasma membrane" evidence=IEA] [GO:0006611 "protein export from nucleus" evidence=IEA] [GO:0030154 "cell differentiation" evidence=IEA] [GO:0030279 "negative regulation of ossification" evidence=IEA] [GO:0030509 "BMP signaling pathway" evidence=IEA] [GO:0030512 "negative regulation of transforming growth factor beta receptor signaling pathway" evidence=IEA] [GO:0030579 "ubiquitin-dependent SMAD protein catabolic process" evidence=IEA] [GO:0032801 "receptor catabolic process" evidence=IEA] [GO:0034394 "protein localization to cell surface" evidence=IEA] [GO:0042787 "protein ubiquitination involved in ubiquitin-dependent protein catabolic process" evidence=IEA] [GO:0043161 "proteasome-mediated ubiquitin-dependent protein catabolic process" evidence=IEA] [GO:0070411 "I-SMAD binding" evidence=IEA] [GO:0070412 "R-SMAD binding" evidence=IEA] Pfam:PF00632 InterPro:IPR000008 InterPro:IPR000569 InterPro:IPR001202 InterPro:IPR024928 Pfam:PF00168 Pfam:PF00397 PIRSF:PIRSF001569 PROSITE:PS01159 PROSITE:PS50004 PROSITE:PS50020 PROSITE:PS50237 SMART:SM00119 SMART:SM00239 SMART:SM00456 UniPathway:UPA00143 GO:GO:0005886 GO:GO:0005737 GO:GO:0030154 GO:GO:0043161 SUPFAM:SSF49562 GO:GO:0030509 GO:GO:0031398 GO:GO:0030279 GO:GO:0004842 GO:GO:0000209 GO:GO:0034394 SUPFAM:SSF51045 GO:GO:0042787 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 GO:GO:0030512 GO:GO:0006611 GO:GO:0032801 GO:GO:0030579 TreeFam:TF323658 OrthoDB:EOG77Q4W4 OMA:GHTPQNR EMBL:AADN03006881 Ensembl:ENSGALT00000007417 Uniprot:F1NFN4)

HSP 1 Score: 577.015 bits (1486), Expect = 0.000e+0
Identity = 262/385 (68.05%), Postives = 324/385 (84.16%), Query Frame = 0
Query:  548 KFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            ++++DLV K+KVLR  L+  QPQ G+C +EVSR+E+FE+SYRQI+KMR K ++K+L VKFR EEGLDYGGVAREWLYLL HEMLNPYYGLFQYS++++Y LQINPDS++NPDHLSYFHFVGRI+G+AVFHGH+I+G FT PFYK +L KPI L D+E+VDP+LHKSL WIL NDI+ ++D+TF VEH AFG    +ELK NG++IPV E NK EYV+LYV +R+  GIE QFL+LQKGFNELIPQ+LLKPFD+KELEL+IGG+  ID+ DWK++TRLK C A++ +V+WFW  V+++  E RARLLQFVTGS+RVPLQGFK LQGSTG  GPR FT+HL +A  D+LPKA TCFNR+D+P Y S+E + EKL  AVEETCGFAVE
Sbjct:  345 RYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCMADSNIVKWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE 729          

HSP 2 Score: 240.736 bits (613), Expect = 4.913e-66
Identity = 150/365 (41.10%), Postives = 190/365 (52.05%), Query Frame = 0
Query:   10 LTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLV----SDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASC---------GTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            +T+LCA+NLAK+D FRLPDPFA++ VD +G  H ++  KNT DPKWN HYDL++   +S T+SVWN KK+HKK   +GFLGCVRL+ NAI R+KDTGYQRLDL     +DT     V+GQIV+SL +RD  GTG   +VVD  G L                 G VY         + + P  P SC          TT     G    + + S++  L           TP   G    P    H   G+ + +LP                                 GY  +TT  GQ+Y++H  TG +TWHDPR+ R L S     LGPLPPGWE R T S R YFV+HNN TTQF+DPR+
Sbjct:   14 ITVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQ-GAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPTDTD---AVRGQIVVSLQTRDRIGTGG--SVVDCRGLL--------------ENEGTVY---------EDSGPGRPLSCFMEEPAPYTDTTGAAGGGNCRFVESPSQDQRLQ-----AQRLRTPEVRGHVQTPQNRPH---GHQSPDLPE--------------------------------GYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRL 309          
BLAST of EMLSAG00000006171 vs. GO
Match: - (symbol:SMURF2 "E3 ubiquitin-protein ligase SMURF2" species:9606 "Homo sapiens" [GO:0000122 "negative regulation of transcription from RNA polymerase II promoter" evidence=TAS] [GO:0000151 "ubiquitin ligase complex" evidence=IDA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=IDA;TAS] [GO:0005160 "transforming growth factor beta receptor binding" evidence=IEA] [GO:0005515 "protein binding" evidence=IPI] [GO:0005634 "nucleus" evidence=NAS;IBA] [GO:0005654 "nucleoplasm" evidence=TAS] [GO:0005737 "cytoplasm" evidence=IBA] [GO:0005829 "cytosol" evidence=TAS] [GO:0005886 "plasma membrane" evidence=IEA] [GO:0006351 "transcription, DNA-templated" evidence=TAS] [GO:0006367 "transcription initiation from RNA polymerase II promoter" evidence=TAS] [GO:0006511 "ubiquitin-dependent protein catabolic process" evidence=IDA] [GO:0007179 "transforming growth factor beta receptor signaling pathway" evidence=IBA;TAS] [GO:0010467 "gene expression" evidence=TAS] [GO:0017015 "regulation of transforming growth factor beta receptor signaling pathway" evidence=NAS] [GO:0030509 "BMP signaling pathway" evidence=TAS] [GO:0030512 "negative regulation of transforming growth factor beta receptor signaling pathway" evidence=TAS] [GO:0030579 "ubiquitin-dependent SMAD protein catabolic process" evidence=IDA] [GO:0042787 "protein ubiquitination involved in ubiquitin-dependent protein catabolic process" evidence=IBA] [GO:0042802 "identical protein binding" evidence=IPI] [GO:0045121 "membrane raft" evidence=IEA] [GO:0045892 "negative regulation of transcription, DNA-templated" evidence=NAS] [GO:0046332 "SMAD binding" evidence=IPI] Reactome:REACT_71 Pfam:PF00632 InterPro:IPR000008 InterPro:IPR000569 InterPro:IPR001202 InterPro:IPR024928 Pfam:PF00168 Pfam:PF00397 PIRSF:PIRSF001569 PROSITE:PS01159 PROSITE:PS50004 PROSITE:PS50020 PROSITE:PS50237 SMART:SM00119 SMART:SM00239 SMART:SM00456 UniPathway:UPA00143 GO:GO:0005829 GO:GO:0005886 Reactome:REACT_111102 Reactome:REACT_116125 Reactome:REACT_6900 GO:GO:0005654 GO:GO:0045121 GO:GO:0000122 SUPFAM:SSF49562 GO:GO:0030509 GO:GO:0007179 GO:GO:0004842 GO:GO:0006367 SUPFAM:SSF51045 eggNOG:COG5021 GO:GO:0042787 SUPFAM:SSF56204 GO:GO:0000151 GO:GO:0030512 GO:GO:0030579 HOVERGEN:HBG004134 HOGENOM:HOG000208451 TreeFam:TF323658 PDB:2DJY PDB:2KXQ PDB:2LTZ PDBsum:2DJY PDBsum:2KXQ PDBsum:2LTZ KO:K04678 OMA:NQAARPF CTD:64750 EMBL:AF310676 EMBL:AF301463 EMBL:AY014180 EMBL:BC093876 EMBL:BC111945 RefSeq:NP_073576.1 UniGene:Hs.515011 PDB:1ZVD PDB:2JQZ PDBsum:1ZVD PDBsum:2JQZ ProteinModelPortal:Q9HAU4 SMR:Q9HAU4 BioGrid:122265 DIP:DIP-33061N IntAct:Q9HAU4 MINT:MINT-1180022 STRING:9606.ENSP00000262435 PhosphoSite:Q9HAU4 DMDM:17865624 PaxDb:Q9HAU4 PRIDE:Q9HAU4 Ensembl:ENST00000262435 Ensembl:ENST00000573633 GeneID:64750 KEGG:hsa:64750 UCSC:uc002jep.1 GeneCards:GC17M062540 HGNC:HGNC:16809 MIM:605532 neXtProt:NX_Q9HAU4 PharmGKB:PA134985524 InParanoid:Q9HAU4 PhylomeDB:Q9HAU4 SignaLink:Q9HAU4 ChiTaRS:SMURF2 EvolutionaryTrace:Q9HAU4 GeneWiki:SMURF2 GenomeRNAi:64750 NextBio:66708 PRO:PR:Q9HAU4 ArrayExpress:Q9HAU4 Bgee:Q9HAU4 CleanEx:HS_SMURF2 Genevestigator:Q9HAU4 Uniprot:Q9HAU4)

HSP 1 Score: 577.4 bits (1487), Expect = 0.000e+0
Identity = 304/625 (48.64%), Postives = 393/625 (62.88%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDAN-----NHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPS-----TSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSN------------ITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T S R  ++NH   TTQ+  P  P    ++     +   D+N+P + + G +  + +   L      S    +  S      P D P      T+ +  +    + +G ++      P  L+  N            I NT    V     NN           + + F    +S++  ++ +       Q      S  P+    +                      +P++K+DLV K+K+LR  L+  QPQ G+C +EVSR+E+FE+SYRQ++KMR K + K+L +KFR EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS +D+YTLQINPDSAVNP+HLSYFHFVGRI+GMAVFHGH+IDG FT PFYK +L K I LDD+E VDPDLH SL WIL NDI+G++D+TF VEH A+G    +ELK NGK IPVNE NK EYV+LYV +R+  GIE QFL+LQKGFNE+IPQ+LLK FDEKELEL+I G+G ID+ DWK +TRLK C  ++ +V+WFW  V+ +  E RARLLQFVTGSSRVPLQGFK LQG+    GPR FT+H  +A  ++LPKA TCFNR+D+P Y S+E + EKL  A+EETCGFAVE
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNN-----------RTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLT---------------------VPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGA---AGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE 748          

HSP 2 Score: 311.997 bits (798), Expect = 3.771e-91
Identity = 180/360 (50.00%), Postives = 223/360 (61.94%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG----LPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
             K+RLT+LCA+NL K+D FRLPDPFA+V VD +G  H ++  KNT DPKWN HYDL++  ++S T+SVWN KK+HKK   +GFLGCVRL+ NAI R+KDTGYQRLDL    K+G      V+GQIV+SL SRD  GTG    VVD   +    NDLP+GWEERRT SGR+ Y+NH+ RTTQW RPT PAS                 SS    LS         +TP S G N        A  G  +D  P +  +   S     + S   R H  T PD LP GY  +TT+ GQ+Y++H  TG +TWHDPR+ R L++     LGPLPPGWE R T + R YFV+HNN TTQF+DPR+
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQ-GAGFLGCVRLLSNAINRLKDTGYQRLDLC---KLGPNDNDTVRGQIVVSLQSRDRIGTGG--QVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPAS---------------EYSSPGRPLS----CFVDENTPIS-GTNG-------ATCGQSSD--PRLAERRVRSQRHRNYMS---RTHLHTPPD-LPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330          
BLAST of EMLSAG00000006171 vs. GO
Match: - (symbol:Smurf2 "E3 ubiquitin-protein ligase" species:10116 "Rattus norvegicus" [GO:0000151 "ubiquitin ligase complex" evidence=IEA] [GO:0004842 "ubiquitin-protein ligase activity" evidence=IBA] [GO:0005634 "nucleus" evidence=IBA] [GO:0005737 "cytoplasm" evidence=IBA] [GO:0007179 "transforming growth factor beta receptor signaling pathway" evidence=IBA] [GO:0030579 "ubiquitin-dependent SMAD protein catabolic process" evidence=IEA] [GO:0042787 "protein ubiquitination involved in ubiquitin-dependent protein catabolic process" evidence=IBA] [GO:0042802 "identical protein binding" evidence=IEA] Pfam:PF00632 InterPro:IPR000008 InterPro:IPR000569 InterPro:IPR001202 InterPro:IPR024928 Pfam:PF00168 Pfam:PF00397 PIRSF:PIRSF001569 PROSITE:PS01159 PROSITE:PS50004 PROSITE:PS50020 PROSITE:PS50237 SMART:SM00119 SMART:SM00239 SMART:SM00456 UniPathway:UPA00143 RGD:1310067 GO:GO:0005634 GO:GO:0005737 SUPFAM:SSF49562 GO:GO:0007179 GO:GO:0004842 SUPFAM:SSF51045 GO:GO:0042787 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 GO:GO:0000151 GO:GO:0030579 OMA:NQAARPF OrthoDB:EOG77Q4W4 EMBL:AABR06065806 EMBL:AABR06065807 EMBL:AABR06065808 EMBL:AABR06065809 EMBL:AABR06065810 Ensembl:ENSRNOT00000019781 NextBio:35580855 PRO:PR:F1M3F2 Uniprot:F1M3F2)

HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0
Identity = 304/625 (48.64%), Postives = 391/625 (62.56%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDAN-----NHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPS-----TSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSN------------ITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T S R  ++NH   TTQ+  P  P    ++     +   D+N+P   + G +  + +   L      S    +  S      P D P      T+ +  +    + +G ++      P  L+  N            I NT    V     NN           + + F    +S++  ++ +       Q      S  P+    +                      +P++K+DLV K+K+LR  L+  QPQ G+C +EVSR+E+FE+SYRQ++KMR K + K+L +KFR EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS +D+YTLQINPDSAVNP+HLSYFHFVGRI+GMAVFHGH+IDG FT PFYK +L K I LDD+E VDPDLH SL WIL NDI+G++D+TF VEH A+G    +ELK NGK IPV E NK EYV+LYV +R+  GIE QFL+LQKGFNE+IPQ+LLK FDEKELEL+I G+G ID+ DWK +TRLK C  ++ VV+WFW  V+ +  E RARLLQFVTGSSRVPLQGFK LQG+    GPR FT+H  +A  ++LPKA TCFNR+D+P Y S+E + EKL  A+EETCGFAVE
Sbjct:  143 LPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNN-----------RTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLT---------------------VPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKANTRLKHCTPDSNVVKWFWKAVELFDEERRARLLQFVTGSSRVPLQGFKALQGA---AGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE 732          

HSP 2 Score: 263.848 bits (673), Expect = 5.594e-74
Identity = 157/355 (44.23%), Postives = 208/355 (58.59%), Query Frame = 0
Query:   11 TILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG----LPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            T+LCA+NL K+D FRLPDPFA+V VD +G  H ++  KNT DPKWN HYDL++  ++S T+S+W  +++ K+ +      C +++ + + R+ D  YQRLDL    K+G      V+GQIV+SL SRD  GTG    VVD   +    NDLP+GWEERRT SGR+ Y+NH+ RTTQW RPT PAS                 SS    LS         +TP + G N        A  G  +D  P +  +   S     + S   R H  T PD LP GY  +TT+ GQ+Y++H  TG +TWHDPR+ R L++     LGPLPPGWE R T + R YFV+HNN TTQF+DPR+
Sbjct:    1 TVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISLWRIREIIKQKHYKFSFEC-KILSSWVGRVLDRFYQRLDLC---KLGPNDNDTVRGQIVVSLQSRDRIGTGG--QVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPAS---------------EYSSPGRPLS----CFVDENTPIT-GTNG-------ATCGQSSD--PRLAERRVRSQRHRNYMS---RTHLHTPPD-LPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 314          
BLAST of EMLSAG00000006171 vs. GO
Match: - (symbol:Smurf2 "SMAD specific E3 ubiquitin protein ligase 2" species:10116 "Rattus norvegicus" [GO:0000151 "ubiquitin ligase complex" evidence=IEA;ISO] [GO:0004842 "ubiquitin-protein ligase activity" evidence=ISO;IBA] [GO:0005160 "transforming growth factor beta receptor binding" evidence=IPI] [GO:0005634 "nucleus" evidence=IBA] [GO:0005737 "cytoplasm" evidence=IBA] [GO:0006511 "ubiquitin-dependent protein catabolic process" evidence=ISO] [GO:0007179 "transforming growth factor beta receptor signaling pathway" evidence=IBA] [GO:0030579 "ubiquitin-dependent SMAD protein catabolic process" evidence=IEA;ISO] [GO:0042787 "protein ubiquitination involved in ubiquitin-dependent protein catabolic process" evidence=IBA] [GO:0042802 "identical protein binding" evidence=IEA;ISO] [GO:0046332 "SMAD binding" evidence=ISO;IPI] Pfam:PF00632 InterPro:IPR000008 InterPro:IPR000569 InterPro:IPR001202 InterPro:IPR024928 Pfam:PF00168 Pfam:PF00397 PIRSF:PIRSF001569 PROSITE:PS01159 PROSITE:PS50004 PROSITE:PS50020 PROSITE:PS50237 SMART:SM00119 SMART:SM00239 SMART:SM00456 UniPathway:UPA00143 RGD:1310067 GO:GO:0005634 GO:GO:0005737 SUPFAM:SSF49562 GO:GO:0007179 GO:GO:0004842 SUPFAM:SSF51045 GO:GO:0042787 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 GO:GO:0000151 GO:GO:0030579 OMA:NQAARPF OrthoDB:EOG77Q4W4 EMBL:AABR06065806 EMBL:AABR06065807 EMBL:AABR06065808 EMBL:AABR06065809 EMBL:AABR06065810 Ensembl:ENSRNOT00000019781 NextBio:35580855 PRO:PR:F1M3F2 Uniprot:F1M3F2)

HSP 1 Score: 576.244 bits (1484), Expect = 0.000e+0
Identity = 304/625 (48.64%), Postives = 391/625 (62.56%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDAN-----NHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPS-----TSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSN------------ITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T S R  ++NH   TTQ+  P  P    ++     +   D+N+P   + G +  + +   L      S    +  S      P D P      T+ +  +    + +G ++      P  L+  N            I NT    V     NN           + + F    +S++  ++ +       Q      S  P+    +                      +P++K+DLV K+K+LR  L+  QPQ G+C +EVSR+E+FE+SYRQ++KMR K + K+L +KFR EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS +D+YTLQINPDSAVNP+HLSYFHFVGRI+GMAVFHGH+IDG FT PFYK +L K I LDD+E VDPDLH SL WIL NDI+G++D+TF VEH A+G    +ELK NGK IPV E NK EYV+LYV +R+  GIE QFL+LQKGFNE+IPQ+LLK FDEKELEL+I G+G ID+ DWK +TRLK C  ++ VV+WFW  V+ +  E RARLLQFVTGSSRVPLQGFK LQG+    GPR FT+H  +A  ++LPKA TCFNR+D+P Y S+E + EKL  A+EETCGFAVE
Sbjct:  143 LPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNN-----------RTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLT---------------------VPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKANTRLKHCTPDSNVVKWFWKAVELFDEERRARLLQFVTGSSRVPLQGFKALQGA---AGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE 732          

HSP 2 Score: 263.848 bits (673), Expect = 5.594e-74
Identity = 157/355 (44.23%), Postives = 208/355 (58.59%), Query Frame = 0
Query:   11 TILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG----LPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            T+LCA+NL K+D FRLPDPFA+V VD +G  H ++  KNT DPKWN HYDL++  ++S T+S+W  +++ K+ +      C +++ + + R+ D  YQRLDL    K+G      V+GQIV+SL SRD  GTG    VVD   +    NDLP+GWEERRT SGR+ Y+NH+ RTTQW RPT PAS                 SS    LS         +TP + G N        A  G  +D  P +  +   S     + S   R H  T PD LP GY  +TT+ GQ+Y++H  TG +TWHDPR+ R L++     LGPLPPGWE R T + R YFV+HNN TTQF+DPR+
Sbjct:    1 TVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISLWRIREIIKQKHYKFSFEC-KILSSWVGRVLDRFYQRLDLC---KLGPNDNDTVRGQIVVSLQSRDRIGTGG--QVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPAS---------------EYSSPGRPLS----CFVDENTPIT-GTNG-------ATCGQSSD--PRLAERRVRSQRHRNYMS---RTHLHTPPD-LPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 314          
BLAST of EMLSAG00000006171 vs. GO
Match: - (symbol:SMURF1 "E3 ubiquitin-protein ligase" species:9823 "Sus scrofa" [GO:0004842 "ubiquitin-protein ligase activity" evidence=IEA] Pfam:PF00632 InterPro:IPR000008 InterPro:IPR000569 InterPro:IPR001202 InterPro:IPR024928 Pfam:PF00168 Pfam:PF00397 PIRSF:PIRSF001569 PROSITE:PS01159 PROSITE:PS50004 PROSITE:PS50020 PROSITE:PS50237 SMART:SM00119 SMART:SM00239 SMART:SM00456 UniPathway:UPA00143 SUPFAM:SSF49562 GO:GO:0004842 SUPFAM:SSF51045 SUPFAM:SSF56204 GeneTree:ENSGT00740000114907 TreeFam:TF323658 OrthoDB:EOG77Q4W4 OMA:GHTPQNR EMBL:FP565331 Ensembl:ENSSSCT00000028241 ArrayExpress:I3LTD6 Uniprot:I3LTD6)

HSP 1 Score: 575.859 bits (1483), Expect = 0.000e+0
Identity = 262/385 (68.05%), Postives = 324/385 (84.16%), Query Frame = 0
Query:  548 KFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            ++++DLV K+KVLR  L+  QPQ G+C +EVSR+E+FE+SYRQI+KMR K ++K+L VKFR EEGLDYGGVAREWLYLL HEMLNPYYGLFQYS++++Y LQINPDS++NPDHLSYFHFVGRI+G+AVFHGH+I+G FT PFYK +L KPI L D+E+VDP+LHKSL WIL NDI+ ++D+TF VEH AFG    +ELK NG+++PV E NK EYV+LYV +R+  GIE QFL+LQKGFNELIPQ+LLKPFD+KELEL+IGG+  ID+ DWK++TRLK C A++ VV+WFW  V+++  E RARLLQFVTGS+RVPLQGFK LQGSTG  GPR FT+HL +A  D+LPKA TCFNR+D+P Y S+E + EKL  AVEETCGFAVE
Sbjct:  347 RYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLDDWKSNTRLKHCGADSNVVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE 731          

HSP 2 Score: 170.629 bits (431), Expect = 1.234e-42
Identity = 118/325 (36.31%), Postives = 161/325 (49.54%), Query Frame = 0
Query:    8 IRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLV----SDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNL---PPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEEL 325
            +   +LCA+NLAK+D FRLPDPFA++ VD +G  H ++  KNT DPKWN HYDL++   +S T+SVWN KK+HKK   +GFLGCVRL+ NA+ R+KDTGYQRLDL     SDT     V+GQIV+SL +RD  GTG    VVD  G L     + E     R  S   +       T          +C   R  +         + +  N        T  + P    +  LP       T  G   +  ++ Q   S+  DP     PR       D L   PPG+ +++T  G+IY++      T + DPRL   +    +L
Sbjct:   15 LEFLVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQ-GAGFLGCVRLLSNAVSRLKDTGYQRLDLCKLNPSDTD---AVRGQIVVSLQTRDRLGTGG--PVVDCRGLLDNEGTVYEDSGPGRPLS--CFMEEPAPYTDGPGAAAGGGNC---RFVESPSQDQRVQAQRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQG--QVYFLHTQTGVSTWHDPRV---PRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQL 323          

HSP 3 Score: 107.457 bits (267), Expect = 1.528e-22
Identity = 59/123 (47.97%), Postives = 74/123 (60.16%), Query Frame = 0
Query:  267 PFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI-PLMNDANNHHHDDNSPTNPSRGTSKNE 388
            P  + Q R H    P+ LP GY  +TT  GQ+Y++H  TG +TWHDPR+ R L S     LGPLPPGWE R T S R YFV+HNN TTQF+DPR+  +MN          S   PS G+ ++E
Sbjct:  220 PLQTPQNRPHGHQSPE-LPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRVPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQSLPLPSEGSVEDE 341          
BLAST of EMLSAG00000006171 vs. C. finmarchicus
Match: gi|592796363|gb|GAXK01158205.1| (TSA: Calanus finmarchicus comp66507_c1_seq4 transcribed RNA sequence)

HSP 1 Score: 594.734 bits (1532), Expect = 0.000e+0
Identity = 291/388 (75.00%), Postives = 334/388 (86.08%), Query Frame = 0
Query:  544 EFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELXXXXXXXXXXXXWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAV 931
            E LP++K+DLVAK+K+LR+ LN+ QP +G+C LE+SR EVFEDSYRQ+VKMR K MRK+L VKF+ EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS ED+YTLQIN DSA+NPDHLSYFHFVGRI+G+A+FHGH+IDG FT PFYKM+LNK I+L DIEAVDP+L  SL W+L NDI+ IID TFSVEH++FG  KV+ELK+NG++  V E NK EYVKLYV +R+  GIEQQFL+LQKGFNELIPQNLLKPFDE+ELELVIGG+GTID+ DWK +TRLK C AE+P V WFW IV+SYS EMRARLLQFVTGSSRVPLQGFK LQGSTG  GPR FTLHL     ++LPKA TCFNRLDLP Y S E M++KLTQAVEETCGFAV
Sbjct: 1864 EILPRYKRDLVAKMKILRTELNSLQPGSGHCRLELSRGEVFEDSYRQVVKMRPKDMRKRLMVKFQGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSREDIYTLQINTDSAINPDHLSYFHFVGRIMGIAMFHGHYIDGGFTMPFYKMLLNKSISLADIEAVDPNLFTSLHWMLENDITDIIDTTFSVEHDSFGAIKVHELKKNGRNFNVTEQNKKEYVKLYVNYRFMHGIEQQFLALQKGFNELIPQNLLKPFDERELELVIGGLGTIDLQDWKTNTRLKHCTAESPQVIWFWRIVESYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHLVEVDTNNLPKAHTCFNRLDLPPYTSEEKMSDKLTQAVEETCGFAV 3027          

HSP 2 Score: 284.263 bits (726), Expect = 1.059e-79
Identity = 137/192 (71.35%), Postives = 155/192 (80.73%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSRDGHGTGSLNA-------VVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPA 190
             KIR+T+LCARNLAKRD+FRLPDPF RV VD +G TH +++ KNT DPKWN HYDL+L  +++ T+SVWNDKK+HKK   +GFLGCVRL+G AI R+KDTGYQRLDLVSD    LPVKGQIV+SLLSRDGHGTGSLNA       VVD LGNL+C  DLPEGWEER T SGRVYYVNH  RTTQW RPT PA
Sbjct:  289 VKIRVTVLCARNLAKRDIFRLPDPFVRVSVDGSGQTHSTDSCKNTLDPKWNQHYDLYLGKSDAVTISVWNDKKVHKKN-GAGFLGCVRLLGAAINRLKDTGYQRLDLVSDATNPLPVKGQIVVSLLSRDGHGTGSLNAVVDPLGQVVDPLGNLSCPLDLPEGWEERVTNSGRVYYVNHYLRTTQWDRPTRPA 861          

HSP 3 Score: 106.301 bits (264), Expect = 2.233e-22
Identity = 51/88 (57.95%), Postives = 63/88 (71.59%), Query Frame = 0
Query:  275 RHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTS-NEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            R  + QP +LP GY M+TT  GQIY+ H G+G ++WHDPRL R   S  E L LG LPPGWE+R T S R YFV+H+N TTQF+DPR+
Sbjct: 1024 RTVLHQPPDLPEGYEMRTTAQGQIYFHHLGSGVSSWHDPRLPRDGESLVEGLELGTLPPGWEKRETSSGRSYFVDHHNRTTQFTDPRL 1287          

HSP 4 Score: 42.743 bits (99), Expect = 6.015e-3
Identity = 18/30 (60.00%), Postives = 22/30 (73.33%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LP GWE+R T SGR Y+V+H  RTTQ+  P
Sbjct: 1192 LPPGWEKRETSSGRSYFVDHHNRTTQFTDP 1281          

HSP 5 Score: 42.3578 bits (98), Expect = 8.233e-3
Identity = 19/33 (57.58%), Postives = 23/33 (69.70%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIP 362
            LP GWE+RVT+S R Y+VNH   TTQ+  P  P
Sbjct:  760 LPEGWEERVTNSGRVYYVNHYLRTTQWDRPTRP 858          
BLAST of EMLSAG00000006171 vs. C. finmarchicus
Match: gi|592796364|gb|GAXK01158204.1| (TSA: Calanus finmarchicus comp66507_c1_seq3 transcribed RNA sequence)

HSP 1 Score: 594.734 bits (1532), Expect = 0.000e+0
Identity = 291/388 (75.00%), Postives = 334/388 (86.08%), Query Frame = 0
Query:  544 EFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELXXXXXXXXXXXXWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAV 931
            E LP++K+DLVAK+K+LR+ LN+ QP +G+C LE+SR EVFEDSYRQ+VKMR K MRK+L VKF+ EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS ED+YTLQIN DSA+NPDHLSYFHFVGRI+G+A+FHGH+IDG FT PFYKM+LNK I+L DIEAVDP+L  SL W+L NDI+ IID TFSVEH++FG  KV+ELK+NG++  V E NK EYVKLYV +R+  GIEQQFL+LQKGFNELIPQNLLKPFDE+ELELVIGG+GTID+ DWK +TRLK C AE+P V WFW IV+SYS EMRARLLQFVTGSSRVPLQGFK LQGSTG  GPR FTLHL     ++LPKA TCFNRLDLP Y S E M++KLTQAVEETCGFAV
Sbjct: 1871 EILPRYKRDLVAKMKILRTELNSLQPGSGHCRLELSRGEVFEDSYRQVVKMRPKDMRKRLMVKFQGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSREDIYTLQINTDSAINPDHLSYFHFVGRIMGIAMFHGHYIDGGFTMPFYKMLLNKSISLADIEAVDPNLFTSLHWMLENDITDIIDTTFSVEHDSFGAIKVHELKKNGRNFNVTEQNKKEYVKLYVNYRFMHGIEQQFLALQKGFNELIPQNLLKPFDERELELVIGGLGTIDLQDWKTNTRLKHCTAESPQVIWFWRIVESYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHLVEVDTNNLPKAHTCFNRLDLPPYTSEEKMSDKLTQAVEETCGFAV 3034          

HSP 2 Score: 284.263 bits (726), Expect = 1.071e-79
Identity = 137/192 (71.35%), Postives = 155/192 (80.73%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSRDGHGTGSLNA-------VVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPA 190
             KIR+T+LCARNLAKRD+FRLPDPF RV VD +G TH +++ KNT DPKWN HYDL+L  +++ T+SVWNDKK+HKK   +GFLGCVRL+G AI R+KDTGYQRLDLVSD    LPVKGQIV+SLLSRDGHGTGSLNA       VVD LGNL+C  DLPEGWEER T SGRVYYVNH  RTTQW RPT PA
Sbjct:  296 VKIRVTVLCARNLAKRDIFRLPDPFVRVSVDGSGQTHSTDSCKNTLDPKWNQHYDLYLGKSDAVTISVWNDKKVHKKN-GAGFLGCVRLLGAAINRLKDTGYQRLDLVSDATNPLPVKGQIVVSLLSRDGHGTGSLNAVVDPLGQVVDPLGNLSCPLDLPEGWEERVTNSGRVYYVNHYLRTTQWDRPTRPA 868          

HSP 3 Score: 106.301 bits (264), Expect = 2.235e-22
Identity = 51/88 (57.95%), Postives = 63/88 (71.59%), Query Frame = 0
Query:  275 RHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTS-NEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            R  + QP +LP GY M+TT  GQIY+ H G+G ++WHDPRL R   S  E L LG LPPGWE+R T S R YFV+H+N TTQF+DPR+
Sbjct: 1031 RTVLHQPPDLPEGYEMRTTAQGQIYFHHLGSGVSSWHDPRLPRDGESLVEGLELGTLPPGWEKRETSSGRSYFVDHHNRTTQFTDPRL 1294          

HSP 4 Score: 42.743 bits (99), Expect = 6.017e-3
Identity = 18/30 (60.00%), Postives = 22/30 (73.33%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LP GWE+R T SGR Y+V+H  RTTQ+  P
Sbjct: 1199 LPPGWEKRETSSGRSYFVDHHNRTTQFTDP 1288          

HSP 5 Score: 42.3578 bits (98), Expect = 8.235e-3
Identity = 19/33 (57.58%), Postives = 23/33 (69.70%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIP 362
            LP GWE+RVT+S R Y+VNH   TTQ+  P  P
Sbjct:  767 LPEGWEERVTNSGRVYYVNHYLRTTQWDRPTRP 865          
BLAST of EMLSAG00000006171 vs. C. finmarchicus
Match: gi|592796365|gb|GAXK01158203.1| (TSA: Calanus finmarchicus comp66507_c1_seq2 transcribed RNA sequence)

HSP 1 Score: 594.734 bits (1532), Expect = 0.000e+0
Identity = 291/388 (75.00%), Postives = 334/388 (86.08%), Query Frame = 0
Query:  544 EFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELXXXXXXXXXXXXWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAV 931
            E LP++K+DLVAK+K+LR+ LN+ QP +G+C LE+SR EVFEDSYRQ+VKMR K MRK+L VKF+ EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS ED+YTLQIN DSA+NPDHLSYFHFVGRI+G+A+FHGH+IDG FT PFYKM+LNK I+L DIEAVDP+L  SL W+L NDI+ IID TFSVEH++FG  KV+ELK+NG++  V E NK EYVKLYV +R+  GIEQQFL+LQKGFNELIPQNLLKPFDE+ELELVIGG+GTID+ DWK +TRLK C AE+P V WFW IV+SYS EMRARLLQFVTGSSRVPLQGFK LQGSTG  GPR FTLHL     ++LPKA TCFNRLDLP Y S E M++KLTQAVEETCGFAV
Sbjct: 1879 EILPRYKRDLVAKMKILRTELNSLQPGSGHCRLELSRGEVFEDSYRQVVKMRPKDMRKRLMVKFQGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSREDIYTLQINTDSAINPDHLSYFHFVGRIMGIAMFHGHYIDGGFTMPFYKMLLNKSISLADIEAVDPNLFTSLHWMLENDITDIIDTTFSVEHDSFGAIKVHELKKNGRNFNVTEQNKKEYVKLYVNYRFMHGIEQQFLALQKGFNELIPQNLLKPFDERELELVIGGLGTIDLQDWKTNTRLKHCTAESPQVIWFWRIVESYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHLVEVDTNNLPKAHTCFNRLDLPPYTSEEKMSDKLTQAVEETCGFAV 3042          

HSP 2 Score: 284.263 bits (726), Expect = 1.089e-79
Identity = 137/192 (71.35%), Postives = 155/192 (80.73%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSRDGHGTGSLNA-------VVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPA 190
             KIR+T+LCARNLAKRD+FRLPDPF RV VD +G TH +++ KNT DPKWN HYDL+L  +++ T+SVWNDKK+HKK   +GFLGCVRL+G AI R+KDTGYQRLDLVSD    LPVKGQIV+SLLSRDGHGTGSLNA       VVD LGNL+C  DLPEGWEER T SGRVYYVNH  RTTQW RPT PA
Sbjct:  289 VKIRVTVLCARNLAKRDIFRLPDPFVRVSVDGSGQTHSTDSCKNTLDPKWNQHYDLYLGKSDAVTISVWNDKKVHKKN-GAGFLGCVRLLGAAINRLKDTGYQRLDLVSDATNPLPVKGQIVVSLLSRDGHGTGSLNAVVDPLGQVVDPLGNLSCPLDLPEGWEERVTNSGRVYYVNHYLRTTQWDRPTRPA 861          

HSP 3 Score: 106.301 bits (264), Expect = 2.240e-22
Identity = 51/88 (57.95%), Postives = 63/88 (71.59%), Query Frame = 0
Query:  275 RHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTS-NEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            R  + QP +LP GY M+TT  GQIY+ H G+G ++WHDPRL R   S  E L LG LPPGWE+R T S R YFV+H+N TTQF+DPR+
Sbjct: 1024 RTVLHQPPDLPEGYEMRTTAQGQIYFHHLGSGVSSWHDPRLPRDGESLVEGLELGTLPPGWEKRETSSGRSYFVDHHNRTTQFTDPRL 1287          

HSP 4 Score: 42.743 bits (99), Expect = 6.020e-3
Identity = 18/30 (60.00%), Postives = 22/30 (73.33%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LP GWE+R T SGR Y+V+H  RTTQ+  P
Sbjct: 1192 LPPGWEKRETSSGRSYFVDHHNRTTQFTDP 1281          

HSP 5 Score: 42.3578 bits (98), Expect = 8.239e-3
Identity = 19/33 (57.58%), Postives = 23/33 (69.70%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIP 362
            LP GWE+RVT+S R Y+VNH   TTQ+  P  P
Sbjct:  760 LPEGWEERVTNSGRVYYVNHYLRTTQWDRPTRP 858          
BLAST of EMLSAG00000006171 vs. C. finmarchicus
Match: gi|592796366|gb|GAXK01158202.1| (TSA: Calanus finmarchicus comp66507_c1_seq1 transcribed RNA sequence)

HSP 1 Score: 594.734 bits (1532), Expect = 0.000e+0
Identity = 291/388 (75.00%), Postives = 334/388 (86.08%), Query Frame = 0
Query:  544 EFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELXXXXXXXXXXXXWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAV 931
            E LP++K+DLVAK+K+LR+ LN+ QP +G+C LE+SR EVFEDSYRQ+VKMR K MRK+L VKF+ EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS ED+YTLQIN DSA+NPDHLSYFHFVGRI+G+A+FHGH+IDG FT PFYKM+LNK I+L DIEAVDP+L  SL W+L NDI+ IID TFSVEH++FG  KV+ELK+NG++  V E NK EYVKLYV +R+  GIEQQFL+LQKGFNELIPQNLLKPFDE+ELELVIGG+GTID+ DWK +TRLK C AE+P V WFW IV+SYS EMRARLLQFVTGSSRVPLQGFK LQGSTG  GPR FTLHL     ++LPKA TCFNRLDLP Y S E M++KLTQAVEETCGFAV
Sbjct: 1886 EILPRYKRDLVAKMKILRTELNSLQPGSGHCRLELSRGEVFEDSYRQVVKMRPKDMRKRLMVKFQGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSREDIYTLQINTDSAINPDHLSYFHFVGRIMGIAMFHGHYIDGGFTMPFYKMLLNKSISLADIEAVDPNLFTSLHWMLENDITDIIDTTFSVEHDSFGAIKVHELKKNGRNFNVTEQNKKEYVKLYVNYRFMHGIEQQFLALQKGFNELIPQNLLKPFDERELELVIGGLGTIDLQDWKTNTRLKHCTAESPQVIWFWRIVESYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHLVEVDTNNLPKAHTCFNRLDLPPYTSEEKMSDKLTQAVEETCGFAV 3049          

HSP 2 Score: 284.263 bits (726), Expect = 1.101e-79
Identity = 137/192 (71.35%), Postives = 155/192 (80.73%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSRDGHGTGSLNA-------VVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPA 190
             KIR+T+LCARNLAKRD+FRLPDPF RV VD +G TH +++ KNT DPKWN HYDL+L  +++ T+SVWNDKK+HKK   +GFLGCVRL+G AI R+KDTGYQRLDLVSD    LPVKGQIV+SLLSRDGHGTGSLNA       VVD LGNL+C  DLPEGWEER T SGRVYYVNH  RTTQW RPT PA
Sbjct:  296 VKIRVTVLCARNLAKRDIFRLPDPFVRVSVDGSGQTHSTDSCKNTLDPKWNQHYDLYLGKSDAVTISVWNDKKVHKKN-GAGFLGCVRLLGAAINRLKDTGYQRLDLVSDATNPLPVKGQIVVSLLSRDGHGTGSLNAVVDPLGQVVDPLGNLSCPLDLPEGWEERVTNSGRVYYVNHYLRTTQWDRPTRPA 868          

HSP 3 Score: 106.301 bits (264), Expect = 2.243e-22
Identity = 51/88 (57.95%), Postives = 63/88 (71.59%), Query Frame = 0
Query:  275 RHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTS-NEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            R  + QP +LP GY M+TT  GQIY+ H G+G ++WHDPRL R   S  E L LG LPPGWE+R T S R YFV+H+N TTQF+DPR+
Sbjct: 1031 RTVLHQPPDLPEGYEMRTTAQGQIYFHHLGSGVSSWHDPRLPRDGESLVEGLELGTLPPGWEKRETSSGRSYFVDHHNRTTQFTDPRL 1294          

HSP 4 Score: 42.743 bits (99), Expect = 6.022e-3
Identity = 18/30 (60.00%), Postives = 22/30 (73.33%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LP GWE+R T SGR Y+V+H  RTTQ+  P
Sbjct: 1199 LPPGWEKRETSSGRSYFVDHHNRTTQFTDP 1288          

HSP 5 Score: 42.3578 bits (98), Expect = 8.242e-3
Identity = 19/33 (57.58%), Postives = 23/33 (69.70%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIP 362
            LP GWE+RVT+S R Y+VNH   TTQ+  P  P
Sbjct:  767 LPEGWEERVTNSGRVYYVNHYLRTTQWDRPTRP 865          
BLAST of EMLSAG00000006171 vs. C. finmarchicus
Match: gi|592943872|gb|GAXK01014681.1| (TSA: Calanus finmarchicus comp9879_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 347.821 bits (891), Expect = 7.336e-103
Identity = 188/387 (48.58%), Postives = 254/387 (65.63%), Query Frame = 0
Query:  549 FKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKM---RYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELXXXXXXXXXXXXWKNHTRLK-QCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFA 930
            + +D   K + L+  L           ++V R  + EDSYRQ+  +   +   ++ KL ++F  E GLDYGGVAREW YLLS EM NPYYGLF+YS+ D YTLQINP+S V N DHL YF F+GR+ GMAV+HG  +DG F  PFYKMML K I L D+E+VD +   SL WI  ND S  ++  F V+ E FG T   EL+  GK+  V + NK EY++L +++R+   + +Q      GFNEL+P NL+K FDE ELEL++ GIG+ID+ DWK +T  K        V+QW+W +V S+S+EMR+RLLQFVTG+SRVP+ GFK+L GS    GP+ FT+    +P +  P+A TCFNRLDLP Y S++ + +KL +A+E + GFA
Sbjct: 1548 YSRDYKRKYEYLKQQLKKPSNVPNKIEIKVRRNTILEDSYRQVHSIPSNKVDTLKTKLWIEFDGEVGLDYGGVAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPNSGVCNEDHLLYFKFIGRVAGMAVYHGKLLDGFFIRPFYKMMLGKSIELKDMESVDTEYFNSLIWIKENDPSE-LELAFQVDEENFGQTVSIELRPGGKETRVTDENKDEYIQLVIEWRFINRVRKQMDQFLAGFNELVPLNLIKIFDEGELELLMCGIGSIDVKDWKGNTVYKGDYHPNHIVIQWYWRVVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGS---NGPQLFTIEKWGSPTN-YPRAHTCFNRLDLPPYESYQQLRDKLIKAIEGSEGFA 2693          

HSP 2 Score: 71.633 bits (174), Expect = 1.011e-11
Identity = 35/89 (39.33%), Postives = 53/89 (59.55%), Query Frame = 0
Query:  282 DNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRA--LTSNEEL-------NLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            + LP G+ M+   +G++++I   + +TTW DPR +R   L S   +       +LGPLP GWE+RV    R +F++HN   TQ+ DPRI
Sbjct: 1248 EGLPSGWTMQVAPNGRVFFIDHISKKTTWVDPRNSRPSPLPSQSNVPNRRHQDDLGPLPEGWEERVHTDGRIFFIDHNTRITQWEDPRI 1514          

HSP 3 Score: 56.225 bits (134), Expect = 4.533e-7
Identity = 24/41 (58.54%), Postives = 28/41 (68.29%), Query Frame = 0
Query:  146 DTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            D+ GN   S  LP GWEER+  +GR YYVNH+ RTTQW  P
Sbjct:  789 DSAGNTNHSVALPAGWEERQDANGRTYYVNHIARTTQWQHP 911          

HSP 4 Score: 52.373 bits (124), Expect = 8.376e-6
Identity = 29/97 (29.90%), Postives = 52/97 (53.61%), Query Frame = 0
Query:    4 RGTKIRLTILCARNLAKRDLFRLPDPFARVDV-----DATGVTHVSEAAKNTTDPKWNTHYDLHLSPNE-SFTLSVWNDKKMHKKTYNSGFLGCVRL 94
            R   +RL ++ A NLAK+D+F   DP+ R+D+     D    + +++  K T +P+W+  +   L P+E    L V+++ ++ +      FLG V L
Sbjct:  198 RTKILRLKVISADNLAKKDIFGASDPYVRIDLVTCRGDEVIDSVLTKTKKRTLNPRWDEEFLFRLKPSEHKLVLEVFDENRLTR----DDFLGRVEL 476          

HSP 5 Score: 42.743 bits (99), Expect = 6.330e-3
Identity = 16/30 (53.33%), Postives = 21/30 (70.00%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LPEGWEER    GR+++++H  R TQW  P
Sbjct: 1419 LPEGWEERVHTDGRIFFIDHNTRITQWEDP 1508          

HSP 6 Score: 37.3502 bits (85), Expect = 3.014e-1
Identity = 12/30 (40.00%), Postives = 20/30 (66.67%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LP GW  +  P+GRV++++H+ + T W  P
Sbjct: 1254 LPSGWTMQVAPNGRVFFIDHISKKTTWVDP 1343          

HSP 7 Score: 36.965 bits (84), Expect = 4.232e-1
Identity = 16/32 (50.00%), Postives = 20/32 (62.50%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            LP GWE+R   + R Y+VNH   TTQ+  P I
Sbjct:  822 LPAGWEERQDANGRTYYVNHIARTTQWQHPGI 917          

HSP 8 Score: 34.2686 bits (77), Expect = 2.494e+0
Identity = 14/36 (38.89%), Postives = 22/36 (61.11%), Query Frame = 0
Query:  325 LNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR 360
            LN+  LP GW  +V  + R +F++H +  T + DPR
Sbjct: 1239 LNIEGLPSGWTMQVAPNGRVFFIDHISKKTTWVDPR 1346          
BLAST of EMLSAG00000006171 vs. C. finmarchicus
Match: gi|592752837|gb|GAXK01201576.1| (TSA: Calanus finmarchicus comp87371_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 342.428 bits (877), Expect = 2.219e-100
Identity = 174/359 (48.47%), Postives = 240/359 (66.85%), Query Frame = 0
Query:  576 LEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGI-IDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELXXXXXXXXXXXXWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDS-LPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            L VSR  +FEDS+ QI+++    +R++L + FR EEGLDYGGVAREW + +SHE+LNP Y LF+Y++++ Y+LQINP S VNPDHL YF F+GR I MA++HG FI   FT PFYK MLN+ + + DIE++DP+ + SL W+  N+I    ++  FSV+ E  G  K ++LK  G DI V E NK EY+ +  ++R   GIE Q  +   GFNE++P   L+ FDE+ELEL++ G+  ID+ DW+ +T  +     +  VQWFW  V +  +E R+RLLQFV G+ RVP+ GF +L GS    GP+ F +       DS LP++ TCFNRLDLP Y S++ + EKLT A+EET GF  E
Sbjct:  371 LSVSRTSLFEDSFHQIMRLPAYELRRRLYIIFRGEEGLDYGGVAREWFFTVSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLHYFKFIGRFIAMALYHGKFIYSGFTMPFYKRMLNRKLTMKDIESIDPEFYNSLIWVRDNNIEECGLEMFFSVDFELLGEIKPHDLKPGGADIQVTEENKEEYLNMVTEWRMTRGIEDQTKAFLDGFNEVVPLEWLQYFDERELELMLCGMQEIDVEDWQKNTIYRHYAKTSKQVQWFWTFVKAMDNETRSRLLQFVCGTCRVPVGGFGELMGS---NGPQRFCIEKVGK--DSWLPRSHTCFNRLDLPPYKSYDQLVEKLTYAIEETEGFGQE 1432          

HSP 2 Score: 72.4034 bits (176), Expect = 5.947e-12
Identity = 41/107 (38.32%), Postives = 54/107 (50.47%), Query Frame = 0
Query:  280 QPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDP---RLTRALT-----------------------SNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR 360
            +P  LPPG+ M+    G++YY+   T QTTW  P   RL +  +                       + E+  LGPLP GWE+RV  + R YFVNH N TTQ+ DPR
Sbjct: 1682 RPQPLPPGWEMRRDPRGRVYYVDHNTRQTTWQRPNTDRLQQMASWQGERARVMQMKNQRFLYPEQQRAAEDDPLGPLPDGWEKRVEPNGRVYFVNHKNRTTQWEDPR 2002          

HSP 3 Score: 60.077 bits (144), Expect = 3.050e-8
Identity = 31/77 (40.26%), Postives = 42/77 (54.55%), Query Frame = 0
Query:  284 LPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR 360
            LP G+  +   +G++Y+++     T W DPR T+     E     PLP GWE R T+    YFV+HN  TT F DPR
Sbjct: 1562 LPDGWEKRVEPNGRVYFVNHKNRTTQWEDPR-TQGQCQEE-----PLPQGWEMRYTEDHVRYFVDHNTRTTTFEDPR 1774          

HSP 4 Score: 56.6102 bits (135), Expect = 3.940e-7
Identity = 28/76 (36.84%), Postives = 38/76 (50.00%), Query Frame = 0
Query:  284 LPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDP 359
            LPPG+ M+  ++G+ YY+   T  TTW  P+              PLPPGWE R     R Y+V+HN   T +  P
Sbjct: 1901 LPPGWEMRYDQYGRRYYVDHNTRSTTWERPQ--------------PLPPGWEMRRDPRGRVYYVDHNTRQTTWQRP 2086          

HSP 5 Score: 51.2174 bits (121), Expect = 1.670e-5
Identity = 21/36 (58.33%), Postives = 25/36 (69.44%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASC 192
            LP+GWE+R  P+GRVY+VNH  RTTQW  P     C
Sbjct: 1667 LPDGWEKRVEPNGRVYFVNHKNRTTQWEDPRTQGQC 1774          

HSP 6 Score: 47.3654 bits (111), Expect = 2.897e-4
Identity = 20/30 (66.67%), Postives = 21/30 (70.00%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LP GWE RR P GRVYYV+H  R T W RP
Sbjct: 1901 LPPGWEMRRDPRGRVYYVDHNTRQTTWQRP 1990          

HSP 7 Score: 38.891 bits (89), Expect = 9.455e-2
Identity = 17/30 (56.67%), Postives = 19/30 (63.33%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LP GWE R    GR YYV+H  R+T W RP
Sbjct: 1997 LPPGWEMRYDQYGRRYYVDHNTRSTTWERP 2086          

HSP 8 Score: 38.1206 bits (87), Expect = 1.641e-1
Identity = 19/43 (44.19%), Postives = 24/43 (55.81%), Query Frame = 0
Query:  318 ALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR 360
            A T  EE    PLPPGWE R     R Y+V+HN  +T +  P+
Sbjct: 1994 AATPAEE----PLPPGWEMRYDQYGRRYYVDHNTRSTTWERPQ 2110          
BLAST of EMLSAG00000006171 vs. C. finmarchicus
Match: gi|592918907|gb|GAXK01039468.1| (TSA: Calanus finmarchicus comp718004_c0_seq4 transcribed RNA sequence)

HSP 1 Score: 325.479 bits (833), Expect = 1.084e-98
Identity = 182/405 (44.94%), Postives = 249/405 (61.48%), Query Frame = 0
Query:  531 QDP--LSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKM---RYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELXXXXXXXXXXXXWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGF 929
            +DP  L+  L G  TE+   +K      +K LR         N    + + R  VF DSY  + K+     K +R +L ++F  E G DYGGV+REW  +LS EM NPYYGLF+YS+ D YTLQINP+S + N DHLS F F+GR+ GMA+ H   ++G F  PFYKMML K INL D+E VD + + SL WI  ND   + + TF VE E FG     EL   GKDI V E NK EY+ L +++R+   + +Q     +GF++++P  ++K FDE ELE ++ GIG ID+ DWK++T  K       V+ WFW +V S+  EMR++LLQF+TG+SRVP  GFK+L GS    GP  F +  + +P +SLP+A TCFNRLDLP Y ++  M +K+  A+E + GF
Sbjct:  309 EDPRFLNPNLAGRKTEYSQDYKYKYEQFMKDLRR--GRTDHINAGLKMRLRRSAVFSDSYNIMKKLDRNEVKRLRNRLWIEFEGEVGYDYGGVSREWFEILSVEMFNPYYGLFEYSAVDNYTLQINPNSGLCNQDHLSVFKFIGRVAGMAIMHKRLLNGFFIRPFYKMMLGKQINLSDMECVDAEYYNSLLWIQDNDPESL-ELTFQVEEEVFGEHTETELVPGGKDIQVTEENKAEYINLVIKWRFISRVREQMTHFLQGFSDILPLRMIKTFDEGELEFLMSGIGVIDVKDWKDNTEYKNYRPTDKVILWFWRMVLSFGDEMRSKLLQFITGTSRVPYNGFKELHGS---NGPCKFKISKSGSP-ESLPRAHTCFNRLDLPEYSTYRNMKKKIVTAIEYSQGF 1502          

HSP 2 Score: 65.4698 bits (158), Expect = 5.266e-10
Identity = 32/97 (32.99%), Postives = 51/97 (52.58%), Query Frame = 0
Query:  280 QPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRL---------------TRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            +P  LPPG++ K    G+ ++I+    +TT+ DPR                T +L+S    NLGPLP GWE+++ +  R +F++H    T + DPR 
Sbjct:   33 EPIPLPPGWVAKIAPSGRTFFINFKINKTTYRDPRTGELTPLPTDSDNENQTISLSSQISDNLGPLPTGWEEKLAEDGRIFFIDHVTKNTSWEDPRF 323          

HSP 3 Score: 40.0466 bits (92), Expect = 3.427e-2
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LP GWEE+    GR+++++HV + T W  P
Sbjct:  228 LPTGWEEKLAEDGRIFFIDHVTKNTSWEDP 317          
BLAST of EMLSAG00000006171 vs. C. finmarchicus
Match: gi|592918909|gb|GAXK01039466.1| (TSA: Calanus finmarchicus comp718004_c0_seq2 transcribed RNA sequence)

HSP 1 Score: 320.857 bits (821), Expect = 1.383e-96
Identity = 170/363 (46.83%), Postives = 233/363 (64.19%), Query Frame = 0
Query:  571 NGYCPLEVSRQEVFEDSY---RQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELXXXXXXXXXXXXWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGF 929
            N    L V R EVF DSY   R++ +   K +R +L ++F  E G DYGGV+REW  +LS EM NPYYGLF+YS+ D YTLQINP+S + N DHL+ F F+GR+ GMA+ H   ++G F  PFYKMML K I + D+E+VD + + SL WI  ND   ++  TF VE E FG     EL   G DI V E NK+EY+ + +++R+   I  Q     +G +++IP  ++K FDE ELE ++ GIG ID+ DWK++T  K       V+ WFW +V S+  EMR++LLQF+TG+SRVP  GFK+L GS    GP  F +  + +P +SLP+A TCFNRLDLP Y ++  M +K+  A+E + GF
Sbjct:  525 NNGVKLRVRRSEVFSDSYSIFRKLGRNEVKRLRNRLWIEFEGEVGYDYGGVSREWFEILSVEMFNPYYGLFEYSAVDNYTLQINPNSGLCNQDHLAVFKFIGRVAGMAIMHKRLLNGFFIRPFYKMMLGKMITISDMESVDAEYYNSLVWIKENDPESLL-LTFQVEEEVFGEHTETELVPGGGDIQVTEENKMEYIDMVIKWRFVSRIRDQMAHFLQGISDIIPLKMIKTFDEGELEFLMSGIGVIDVKDWKDNTEYKNYRPTDKVILWFWRMVLSFGDEMRSKLLQFITGTSRVPYNGFKELHGS---NGPCKFKISKSGSP-ESLPRAHTCFNRLDLPEYSTYRNMKKKIVTAIEYSQGF 1598          

HSP 2 Score: 65.0846 bits (157), Expect = 7.053e-10
Identity = 32/90 (35.56%), Postives = 52/90 (57.78%), Query Frame = 0
Query:  284 LPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRA----LTSNEE--------LNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            LPPG++ +    G+I++I+    +TT+ DPR  +     L+S+ E         +LGPLP GWE+R+++  R +F+NH    T + DPR 
Sbjct:  150 LPPGWVSQVAPTGRIFFINFRNNKTTFRDPRTGQITPMPLSSDAENQKMSLSLDSLGPLPTGWEERLSEDGRIFFINHTKKKTTWEDPRF 419          

HSP 3 Score: 46.595 bits (109), Expect = 3.619e-4
Identity = 24/66 (36.36%), Postives = 35/66 (53.03%), Query Frame = 0
Query:  121 PVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            P  GQI    LS D      ++  +D+LG       LP GWEER +  GR++++NH ++ T W  P
Sbjct:  237 PRTGQITPMPLSSDAENQ-KMSLSLDSLG------PLPTGWEERLSEDGRIFFINHTKKKTTWEDP 413          

HSP 4 Score: 42.743 bits (99), Expect = 5.848e-3
Identity = 17/36 (47.22%), Postives = 23/36 (63.89%), Query Frame = 0
Query:  325 LNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR 360
            +N  PLPPGW  +V  + R +F+N  N+ T F DPR
Sbjct:  135 INETPLPPGWVSQVAPTGRIFFINFRNNKTTFRDPR 242          

HSP 5 Score: 35.4242 bits (80), Expect = 1.059e+0
Identity = 13/33 (39.39%), Postives = 22/33 (66.67%), Query Frame = 0
Query:  284 LPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLT 316
            LP G+  + +E G+I++I+    +TTW DPR +
Sbjct:  324 LPTGWEERLSEDGRIFFINHTKKKTTWEDPRFS 422          
BLAST of EMLSAG00000006171 vs. C. finmarchicus
Match: gi|592918910|gb|GAXK01039465.1| (TSA: Calanus finmarchicus comp718004_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 329.331 bits (843), Expect = 1.868e-96
Identity = 182/405 (44.94%), Postives = 250/405 (61.73%), Query Frame = 0
Query:  531 QDP--LSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKM---RYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELXXXXXXXXXXXXWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGF 929
            +DP  L+  L G  TE+   +K      +K LR    +    N    + + R  VF DSY  + K+     K +R +L ++F  E G DYGGV+REW  +LS EM NPYYGLF+YS+ D YTLQINP+S + N DHLS F F+GR+ GMA+ H   ++G F  PFYKMML K INL D+E VD + + SL WI  ND   + + TF VE E FG     EL   GKDI V E NK EY+ L +++R+   + +Q     +GF++++P  ++K FDE ELE ++ GIG ID+ DWK++T  K       V+ WFW +V S+  EMR++LLQF+TG+SRVP  GFK+L GS    GP  F +  + +P +SLP+A TCFNRLDLP Y ++  M +K+  A+E + GF
Sbjct: 1427 EDPRFLNPNLAGRKTEYSQDYKYKYEQFMKDLRRGRTDHI--NAGLKMRLRRSAVFSDSYNIMKKLDRNEVKRLRNRLWIEFEGEVGYDYGGVSREWFEILSVEMFNPYYGLFEYSAVDNYTLQINPNSGLCNQDHLSVFKFIGRVAGMAIMHKRLLNGFFIRPFYKMMLGKQINLSDMECVDAEYYNSLLWIQDNDPESL-ELTFQVEEEVFGEHTETELVPGGKDIQVTEENKAEYINLVIKWRFISRVREQMTHFLQGFSDILPLRMIKTFDEGELEFLMSGIGVIDVKDWKDNTEYKNYRPTDKVILWFWRMVLSFGDEMRSKLLQFITGTSRVPYNGFKELHGS---NGPCKFKISKSGSP-ESLPRAHTCFNRLDLPEYSTYRNMKKKIVTAIEYSQGF 2620          

HSP 2 Score: 65.0846 bits (157), Expect = 9.983e-10
Identity = 32/97 (32.99%), Postives = 52/97 (53.61%), Query Frame = 0
Query:  280 QPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRL---------------TRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            +P  LPPG++ K    G+ ++I+    +TT+ DPR                T +L+S    NLGPLP GWE+++++  R +F++H    T + DPR 
Sbjct: 1151 EPIPLPPGWVAKIAPSGRTFFINFKINKTTYRDPRTGELTPLPTDSDNENQTISLSSQISDNLGPLPTGWEEKLSEDGRIFFIDHVTKNTSWEDPRF 1441          

HSP 3 Score: 57.7658 bits (138), Expect = 1.740e-7
Identity = 32/103 (31.07%), Postives = 49/103 (47.57%), Query Frame = 0
Query:  279 TQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALT------------SNEELNLG---------PLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR 360
            ++P  LPPG++      G++Y+ + G  QTTW DPR     +            S+  L+L          PLPPGW  ++  S R +F+N   + T + DPR
Sbjct:  947 SRPLPLPPGWVANLAPSGRVYFTNFGNRQTTWTDPRTGEPTSFPSTSQNVAEPNSDHALDLDGATRTEEPIPLPPGWVAKIAPSGRTFFINFKINKTTYRDPR 1255          

HSP 4 Score: 56.6102 bits (135), Expect = 3.408e-7
Identity = 53/201 (26.37%), Postives = 91/201 (45.27%), Query Frame = 0
Query:    8 IRLTILCARNLAKRDLFRLPDPFARVDVD---ATGVTHV-------SEAAKNTT-----DPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRL----MGNAIQRIKDTGYQRLDLVSDTKIGLPVKGQIVI--SLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPT 187
            I+L I  A NLA++D+F   DP+  +  +    T  T V       +E  + TT     +P+WN  +++ +   +     V++  ++ +      FLG   L    +G+ +     +G  RL   ++      V+G + I  S +    +   S     D +   T  + LP GWEER   +GR+ Y +H  RTTQ+ RP+
Sbjct:   44 IKLVIEGAINLARKDIFGASDPYVIIYKENDFTTSHTAVPLQFSPSNEVGRTTTKSRTLNPRWNESFNIKIEAGKVLIFEVFDKNRLTR----DDFLGRTHLKPISVGSTV-----SGTLRLYKRNERS---RVRGDLYINYSWVEETLNDIESAPENDDRIVPTTTDDSLPGGWEERHDNNGRMVYFHHQSRTTQFGRPS 610          

HSP 5 Score: 40.4318 bits (93), Expect = 3.521e-2
Identity = 13/30 (43.33%), Postives = 21/30 (70.00%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LP GWEE+ +  GR+++++HV + T W  P
Sbjct: 1346 LPTGWEEKLSEDGRIFFIDHVTKNTSWEDP 1435          

HSP 6 Score: 35.8094 bits (81), Expect = 8.015e-1
Identity = 18/37 (48.65%), Postives = 19/37 (51.35%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARPT--EPAS 191
            LP GW     PSGRVY+ N   R T W  P   EP S
Sbjct:  962 LPPGWVANLAPSGRVYFTNFGNRQTTWTDPRTGEPTS 1072          

HSP 7 Score: 32.7278 bits (73), Expect = 6.865e+0
Identity = 13/32 (40.62%), Postives = 20/32 (62.50%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            LP GWE+R  ++ R  + +H + TTQF  P +
Sbjct:  518 LPGGWEERHDNNGRMVYFHHQSRTTQFGRPSL 613          
BLAST of EMLSAG00000006171 vs. C. finmarchicus
Match: gi|592858480|gb|GAXK01099082.1| (TSA: Calanus finmarchicus comp239060_c0_seq1 transcribed RNA sequence)

HSP 1 Score: 305.834 bits (782), Expect = 6.891e-87
Identity = 173/382 (45.29%), Postives = 244/382 (63.87%), Query Frame = 0
Query:  553 LVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMR-YKV--MRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELXXXXXXXXXXXXWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFA 930
             + K++ L S  N  +       L+V R E+  DS+  I  ++ ++V  +R KL + F  E+GLDYGG+AREW   L+ ++ NPYYGLF+YS+ D YTLQIN +S + N +HL YF F+GRI+GMAVFH   I+G F  PFY MML K I L D+E+VD + + SL WI  ND    +  TF V+ E FG     +L   G DI V E NK+EY++  +++R+   ++ Q   L +GF+++IP   +  FDE ELEL++GGIG I++ DW+++T  K C     V+ WFW +V S+  E R+RLLQFVTG+SRVP+ GF +L GS    GP+ FT+     P +SLP+A TCFNR+DLP Y SFE +  K+  A+E + GF+
Sbjct: 1951 FIRKMEALGSKTNKLE-------LKVRRNEIVNDSFNLIGTLKGHEVGKLRNKLWIVFEGEQGLDYGGLAREWFNNLTTDIFNPYYGLFEYSATDNYTLQINSNSGLCNEEHLLYFRFIGRIVGMAVFHKKLINGFFIRPFYSMMLGKRITLKDMESVDAEYYNSLVWIKDND-PECLALTFQVDDEVFGEQVQEDLVSGGGDIDVTEENKMEYIECVIKWRFVSRVQDQMNQLMEGFHDVIPMGSINSFDEGELELLLGGIGCINVKDWRDNTEYKNCTPNERVILWFWRLVLSFGDEKRSRLLQFVTGTSRVPMNGFSELHGS---NGPKKFTIEKIGNP-ESLPRAHTCFNRIDLPDYRSFEELKNKVVTAIEGSWGFS 3060          

HSP 2 Score: 59.6918 bits (143), Expect = 4.305e-8
Identity = 32/90 (35.56%), Postives = 46/90 (51.11%), Query Frame = 0
Query:  284 LPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRA------------LTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            LPPG+ MK T  G+ ++I   T  T+W DPR TR+              + +   LGPLP GW ++   + R +FV+H    T + DPR 
Sbjct: 1618 LPPGWTMKVTPEGRPFFIDHTTRTTSWTDPR-TRSESTAASASAPSPTPTQDSRGLGPLPEGWVEKTLPNGRIFFVDHITQKTTWEDPRF 1884          

HSP 3 Score: 48.521 bits (114), Expect = 1.214e-4
Identity = 19/40 (47.50%), Postives = 27/40 (67.50%), Query Frame = 0
Query:  321 SNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR 360
            ++E  N  PLPPGW  +VT   RP+F++H   TT ++DPR
Sbjct: 1591 ASERRNSEPLPPGWTMKVTPEGRPFFIDHTTRTTSWTDPR 1710          

HSP 4 Score: 45.0542 bits (105), Expect = 1.445e-3
Identity = 18/34 (52.94%), Postives = 23/34 (67.65%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPA 190
            LP GW+ RR  +GRV+Y+NH  R TQW  P E +
Sbjct: 1204 LPLGWDTRRDNNGRVFYINHETRRTQWEMPREQS 1305          

HSP 5 Score: 41.9726 bits (97), Expect = 1.266e-2
Identity = 14/30 (46.67%), Postives = 22/30 (73.33%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            LPEGW E+  P+GR+++V+H+ + T W  P
Sbjct: 1789 LPEGWVEKTLPNGRIFFVDHITQKTTWEDP 1878          

HSP 6 Score: 40.0466 bits (92), Expect = 5.043e-2
Identity = 17/38 (44.74%), Postives = 24/38 (63.16%), Query Frame = 0
Query:  323 EELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR 360
            EE NLG LP GW+ R  ++ R +++NH    TQ+  PR
Sbjct: 1183 EESNLGILPLGWDTRRDNNGRVFYINHETRRTQWEMPR 1296          

HSP 7 Score: 38.891 bits (89), Expect = 1.119e-1
Identity = 15/33 (45.45%), Postives = 20/33 (60.61%), Query Frame = 0
Query:  154 SNDLPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            S  LP GW  + TP GR ++++H  RTT W  P
Sbjct: 1609 SEPLPPGWTMKVTPEGRPFFIDHTTRTTSWTDP 1707          

HSP 8 Score: 38.5058 bits (88), Expect = 1.197e-1
Identity = 15/42 (35.71%), Postives = 27/42 (64.29%), Query Frame = 0
Query:  284 LPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEEL 325
            LP G++ KT  +G+I+++   T +TTW DPR +    + +E+
Sbjct: 1789 LPEGWVEKTLPNGRIFFVDHITQKTTWEDPRFSNPDIAGKEV 1914          

HSP 9 Score: 38.5058 bits (88), Expect = 1.544e-1
Identity = 24/97 (24.74%), Postives = 47/97 (48.45%), Query Frame = 0
Query:    8 IRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHV---------SEAAKNTTDPKWNTHYDLHLSPNE-SFTLSVWNDKKMHKKTYNSGFLGCVRL 94
            +RLTI  A +L K+D+F   DP+ ++  +      V         +   + T +P+W+  +++ + P +    L V+++ +M +      FLG V L
Sbjct:  241 LRLTIYKACDLVKKDIFGASDPYVQIYREMINNYEVQSPSQKIRKTATIRKTVNPEWHETFEIEVDPQKHELVLDVFDENRMTR----DDFLGRVTL 519          
BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Match: EMLSAP00000006171 (pep:novel supercontig:LSalAtl2s:LSalAtl2s336:651240:657086:1 gene:EMLSAG00000006171 transcript:EMLSAT00000006171 description:"maker-LSalAtl2s336-augustus-gene-6.36")

HSP 1 Score: 1917.89 bits (4967), Expect = 0.000e+0
Identity = 932/932 (100.00%), Postives = 932/932 (100.00%), Query Frame = 0
Query:    1 MEIRGTKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            MEIRGTKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE
Sbjct:    1 MEIRGTKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932          
BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Match: EMLSAP00000007514 (pep:novel supercontig:LSalAtl2s:LSalAtl2s431:6204:41118:1 gene:EMLSAG00000007514 transcript:EMLSAT00000007514 description:"maker-LSalAtl2s431-snap-gene-0.13")

HSP 1 Score: 357.451 bits (916), Expect = 9.630e-109
Identity = 186/388 (47.94%), Postives = 251/388 (64.69%), Query Frame = 0
Query:  549 FKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKM----RYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLK-QCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFA 930
            + +D   K + L+S L           +++ R+ + EDS+++I       +  V++ KL ++F  E GLDYGGVAREW YLLS EM NPYYGLF+YS+ D YTLQINP S V N DHLSYF F+GR  GMA   G  +DG F  PFYKMML K + L D+E+VD +   SL WI  ND   + + TF V+ E FG T   ELK  GK+IPV   NK EY+   +Q+R+   I++Q  +   GFNEL+P + +K FDE ELEL++ GIG+ID+ DWK +T  K        V+QW+W +V S+++EMR+RLLQFVTG+SRVP+ GFK+L GS    GP+ FT+     P  S P+A TCFNRLDLP Y S++ + ++L +A+E + GFA
Sbjct:  367 YNRDYKRKYEFLKSQLKKPSNVPNKFEIKIRRRYILEDSFQKIFMQFPSHKVDVLKTKLWIEFDGEIGLDYGGVAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGVCNEDHLSYFKFIGRXAGMAXXXGKLLDGFFIRPFYKMMLGKGMELSDMESVDTEXFNSLMWIRENDPQEL-ELTFQVDEENFGTTVTRELKPGGKNIPVTNENKDEYIHQVIQWRFVSRIQKQMDNFLAGFNELVPLSFIKIFDEGELELLMCGIGSIDVKDWKQNTVYKGDYHPNHIVIQWYWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGS---NGPQLFTIERWGNP-SSYPRAHTCFNRLDLPPYESYQQLRDRLIKAIEGSEGFA 749          

HSP 2 Score: 73.9442 bits (180), Expect = 1.229e-13
Identity = 37/84 (44.05%), Postives = 51/84 (60.71%), Query Frame = 0
Query:  287 GYIMKTTEHGQIYYIHEGTGQTTWHDPRLTR--ALTSN-------EELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            G+ M+   +G+++YI+    +TTW DPR  R  AL S         E +LGPLP GWE+RV    R +F++HN  TTQ+ DPRI
Sbjct:  272 GWTMQVAPNGRVFYINHADKETTWVDPRSGRPSALPSQANVPNRRHEDDLGPLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRI 355          
BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Match: EMLSAP00000011039 (pep:novel supercontig:LSalAtl2s:LSalAtl2s744:109377:113126:-1 gene:EMLSAG00000011039 transcript:EMLSAT00000011039 description:"maker-LSalAtl2s744-snap-gene-1.41")

HSP 1 Score: 347.821 bits (891), Expect = 1.178e-104
Identity = 173/379 (45.65%), Postives = 241/379 (63.59%), Query Frame = 0
Query:  576 LEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAR--------------------EWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGI-IDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDS-LPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            L V+RQ +FEDS+ Q++++    +R++L + FR EEGLDYGG AR                    EW +LLSHE+LNP Y LF+Y++++ Y+LQINP S VNPDHL YF F+GR I MA++HG FI   FT PFYK MLNK + + DIE++DP+ + SL WI  N+I    ++  F V+ E  G  K +ELK  G ++ V E NK EY+++  ++R   GIE+Q  S  +GFNE++P   L+ FDE+ELEL++ G+  ID+ DW+ H+  +     +  +QWFW  V S   E R+RLLQFV G+ RVP+ GF +L GS    G + F +       DS LP++ TCFNRLDLP Y S++ + EKL  A+EET GF +E
Sbjct:  502 LSVTRQTLFEDSFHQLMRLPAFELRRRLYIIFRGEEGLDYGGXARXVDMLLLLPCSYVNLIYPLREWFFLLSHEVLNPMYCLFEYANKNNYSLQINPASYVNPDHLHYFKFIGRFIAMALYHGKFIYSGFTMPFYKRMLNKKLIMKDIESIDPEYYNSLMWIKDNNIEECGLEMYFCVDFEVLGEIKPHELKPGGSELKVTEENKEEYIEIVCEWRMSRGIEEQTKSFLEGFNEVVPLEWLQYFDERELELMLCGMQVIDVDDWQRHSIYRHYTKNSKQIQWFWQFVRSMDHEKRSRLLQFVCGTCRVPVGGFAELMGS---NGAQRFCVEKVGK--DSWLPRSHTCFNRLDLPPYKSYDQLVEKLNFAIEETEGFGLE 875          

HSP 2 Score: 72.7886 bits (177), Expect = 2.941e-13
Identity = 61/222 (27.48%), Postives = 88/222 (39.64%), Query Frame = 0
Query:  140 SLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSK-TTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR 360
            S NA   +         LP GWE R     R YYV+H  R+T W RP         RR +RG    + ++++     +         ++   E A  L + +   L            +Q  PS   DP   L               G+  +   +G++Y+++     T W DPR      +  ++   PLPPGWE R      PYFV+HN  TT F DPR
Sbjct:  265 SANANSSSRATAATEEPLPPGWEMRYDRYRRXYYVDHNNRSTTWERPQPLPVGWEMRRDERGRIYYVDHNTRQTTWQRPNVDRLQNFASWQGERAQVLQMRNQRFL----------YPDQSRPSE-EDPQGPLMD-------------GWEKRVEPNGRVYFVNHKNRTTQWEDPR------TQGQVKEDPLPPGWEIRFMAGGVPYFVDHNTRTTTFQDPR 456          
BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Match: EMLSAP00000004991 (pep:novel supercontig:LSalAtl2s:LSalAtl2s260:299952:311628:1 gene:EMLSAG00000004991 transcript:EMLSAT00000004991 description:"maker-LSalAtl2s260-augustus-gene-3.39")

HSP 1 Score: 282.337 bits (721), Expect = 1.165e-78
Identity = 136/358 (37.99%), Postives = 217/358 (60.61%), Query Frame = 0
Query:  576 LEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGI-IDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            + V R+ VFE+S+R++ +   +  + +  + F  EEG D GG+ REW  ++S E+ NP Y LF+ S  D  T  I   S  NP+HL YF FVGR+I  A++    ++  FT  FYK +L KP+   D+E+ D   +K L++++++ +S +  D TFS E + FG+T++ +L  NG++I V E  K++Y++L  Q +    I +Q  +  +GF  +IP+ L+  F+E+ELEL++ G+ TIDI D K++T   +    +  + WFW  + S+    +A+ +QFVTGSS+VPLQGF  L+   G  G + F +H  +   D LP A TCFN+LDLPAY +++ +   L +A+ E C   +E
Sbjct: 2603 VHVRRENVFEESFRELXRRSPEEXKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYESSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILGKPVKYTDMESEDYAFYKGLEFLVNHKVSDLGYDLTFSTEIQEFGVTEIRDLIPNGRNITVTEETKLDYIRLVCQMKMTGAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPTIDIDDLKSNTEYHKYQRNSLQIIWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFAYLE---GMNGVQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRTYLLKAIHE-CSEVME 2956          
BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Match: EMLSAP00000003493 (pep:novel supercontig:LSalAtl2s:LSalAtl2s190:418567:426512:1 gene:EMLSAG00000003493 transcript:EMLSAT00000003493 description:"maker-LSalAtl2s190-snap-gene-4.16")

HSP 1 Score: 176.407 bits (446), Expect = 1.769e-45
Identity = 118/366 (32.24%), Postives = 195/366 (53.28%), Query Frame = 0
Query:  578 VSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRN-----EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPD-SAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNK---PINLDDIEAVDPDLHKSLKWILSNDISGIIDNT---FSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGT-IDIVDWKNHTRLKQCDA-ETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGF 929
            + R+ ++ED+  ++       M+ K+RV+F+N     E G+D GG+ RE+L  L     +P  G F+ + +    L  NP    +  D L ++ F+GR++G A++    ++    + F   +L K    +++D ++++DP+L+++L ++ + D  G + +    F++ +E  G T V ELK NG  IPV   N+ EY+ L   ++    I  Q  + ++G +++I  + LK F+ KEL+LVI G  T ID+ D +NHT      A + P V  FW +V+S +   +  LL+FVT  SR PL GFK+L           F +H   A    LP A TC N L LP +   +I+ +KL  A+E   GF
Sbjct:  660 IRRKFIYEDALEKLSPEAEPNMKLKMRVEFKNIAGLREAGIDGGGLFREFLGELVKTSFDPNRGFFRLTKDQ--RLYPNPSVHKIYEDFLPHYFFIGRMLGKALYENMLVEFPLASFFLHKLLEKSSVKVDIDHLQSLDPELYRNLLYLKNYD--GCVQDLGLDFTIVNEEVGTTSVEELKRNGSSIPVTNVNRTEYIHLIANYKLNKQIRLQSNAFRQGLSDVINLDWLKMFNYKELQLVISGAETEIDVSDLRNHTNYTGGYAVDHPTVVIFWNVVESMTETQKRHLLKFVTSCSRPPLLGFKELDPP--------FCVHNAGAEPHRLPTASTCMNLLKLPNFEEEKILKDKLLYAIESGSGF 1013          
BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Match: EMLSAP00000008825 (pep:novel supercontig:LSalAtl2s:LSalAtl2s54:414186:494517:-1 gene:EMLSAG00000008825 transcript:EMLSAT00000008825 description:"maker-LSalAtl2s54-augustus-gene-5.10")

HSP 1 Score: 159.458 bits (402), Expect = 5.573e-40
Identity = 113/364 (31.04%), Postives = 184/364 (50.55%), Query Frame = 0
Query:  576 LEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRN-----EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPIN-----LDDIEAVDPDLHKSLKWILSNDISGI-IDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKE-LELVIGGIGTIDIVDWKNHTRLKQCDAET-PVVQWFWAIVD-SYSSEMRARLLQFVTGSSRVPLQGFKDLQ----------------GSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAY 909
            + V R  + ED YR + K+  + ++  +RVKF N     E G+D  GV +E+L      + +P   LF  +S++   L  +P S +  ++L  F FVG++IG AV+ G  ++  F   F   +L+K  +     LD++ ++DP+L+K+L +I   D +   +D TFS + +  G    +EL E G+ + VN+ NK+ Y+    QF     I++Q  +  +GF  ++P + L  F   E L L+ G    +D+ D + HT       ++  V+ W W I+D  +  + R+  L+FVT  S+ PL GF++L+                G T  +  R F       PV+ LP A TCFN L LP Y
Sbjct:  615 ITVHRSRIVEDGYRHLGKLSSRSLKGVIRVKFINTQGLDEAGIDQDGVFKEFLEETIKRVFDPGLNLFCSTSDN--RLYPSPTSHLTENYLQLFEFVGKMIGKAVYEGIVVEIPFAPFFISQILSKDHSAYYSYLDELPSMDPELYKNLTYIKYFDGNVTDLDLTFSYDQDVLGRVVTHELIEGGRSVLVNDCNKISYIHHVAQFVLHSQIKEQVAAFTRGFRAILPIDWLNLFSPPEVLRLISGDNLPLDLKDLRRHTHYYGGFHDSHKVIVWLWEILDRDFDDKERSSFLKFVTSCSKPPLLGFENLEPAFSIRCVEVAEDEDDGDTVGSVLRGFLALKKRDPVNRLPTASTCFNLLKLPNY 976          
BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Match: EMLSAP00000009470 (pep:novel supercontig:LSalAtl2s:LSalAtl2s60:50964:63500:-1 gene:EMLSAG00000009470 transcript:EMLSAT00000009470 description:"maker-LSalAtl2s60-augustus-gene-0.20")

HSP 1 Score: 115.161 bits (287), Expect = 3.558e-26
Identity = 87/299 (29.10%), Postives = 135/299 (45.15%), Query Frame = 0
Query:  664 FHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKP--INLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEH-----------------------EAFGLTKVY------ELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQC------DAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEE 925
            F F+G+ I  AV     ID  F+ PFY+ +L +   + + D+  VDP +  ++     + + GI      +E                        E  GL          +L++ GKDIPVN  N  +YV L   +    GI  Q  +L+ GF  + P + L+ F  +ELE +  G G  +   W   T ++        ++E+P +++   +++SY+ + + + LQFVTG  R+P+ GFK L      T  +    +    P D LP   TC N L LP Y S +IM EKL  A  E
Sbjct: 1444 FTFLGKFIAKAVLDNRMIDMPFSQPFYQWLLREESTLGISDLRNVDPTIASTI-----SSLEGIARKKRKLEQDPQISPDEKLEQIKSLTMDGCPIEDLGLDFTLPGYPNIDLRKGGKDIPVNIDNLHQYVGLVSHWLLIEGISFQMEALRDGFESVFPISTLQMFYPEELEQIFCGTGQGNFNKWDYKTLVESTKPDHGFNSESPALKYLLEVLESYNIDEQRQFLQFVTGCPRLPVGGFKALNPPL--TIVKKSFDNADADPDDYLPSVMTCVNYLKLPDYSSKKIMREKLRIAAME 1735          
BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Match: EMLSAP00000007808 (pep:novel supercontig:LSalAtl2s:LSalAtl2s458:200727:208701:-1 gene:EMLSAG00000007808 transcript:EMLSAT00000007808 description:"maker-LSalAtl2s458-snap-gene-2.45")

HSP 1 Score: 109.768 bits (273), Expect = 7.173e-25
Identity = 87/326 (26.69%), Postives = 141/326 (43.25%), Query Frame = 0
Query:  603 LRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKS-LKWILSNDISGIIDNTFS--VEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEE 925
            L++ F  E+G++YGG+ REW  LL  E+           S+    +     +  NP    Y  F                  F++ F   +    I     +   P+ + S + +I+ N +  + D  F+  +  E   L +   LK +GK I V   NK EY+ L  ++R    I+      + G +  + +  L  FDE E++L+  G  TID  D K H   +  +    +++WFW +++  S E +   L+F+TGSSR+P  GFKDL           FT+   N     LP +  C N L++  + SFE        A+ E
Sbjct:  385 LKISFSGEQGIEYGGLRREWYTLLWKEL-----------SDTKIFITNERGTGANPMFRLYLPF-----------------SFSSSFLYQLKGINIGXRHFKVDYPEFYTSRIDYIVKNKVEDL-DLYFTDEIYDEKGHLIESKNLKPSGKKIKVTNFNKFEYLHLLAKYRLSDRIKGPLTKFKTGLHYYVSETYLANFDESEMDLLFSGFSTIDFNDLKKHHNCQ--NVSHKIMEWFWLVLEHLSEEQKLLFLKFITGSSRLPPNGFKDLCPV--------FTIRQLNEE-GKLPSSHVCANVLNIHPHSSFEAFESSFITAITE 670          
BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Match: EMLSAP00000002245 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1426:7958:26313:1 gene:EMLSAG00000002245 transcript:EMLSAT00000002245 description:"maker-LSalAtl2s1426-augustus-gene-0.12")

HSP 1 Score: 98.5969 bits (244), Expect = 5.431e-21
Identity = 97/400 (24.25%), Postives = 177/400 (44.25%), Query Frame = 0
Query:  557 IKVLRSXLNNXQPQNGYCPLEVSRQEVF----EDSYRQIVKMRYKVMRKKLRVKFRN-------EEGLDYGGVAREWLYLLSHEMLNPYYGLFQ---------YSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLT---KVYELKENGKDIPV---------NESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVE 924
            ++ L S + N      Y P +V+ + +F       + QI +  + +  K+LR+ +R+       E   D GGV  + +  +  E+      L            S++D Y L  + +S V+  H   F F+G + G+A+     I        +K++L       D+E VD    +SL ++ +         +F+ ++E   L        +  +G+++P+         +  NK EYVK  +  R+   +  Q L+++KG + L P  +LK    + +E ++ G  +  +   K   R +  D    V+ W W I++ ++++++   L FV+G SR+P   F   Q          F +     P + LP A+TCF +L LP Y S EIM  +L  A++
Sbjct: 2955 MQTLMSTMTNSNVLRNYGP-QVTVRRLFPIGSSSIFSQIAEQVFYIDPKELRLPYRSWKVKLVGEAADDAGGVFDDTISEMCQELSKDVLNLLTPTPNGISDIGSNQDKYLLNSDKNSKVDMRH---FWFLGVLCGVALRTRKPIAINLAPLVWKLILEIKTTWKDLEDVDLSYSRSLHYVAT---------SFNPDNEEDSLLPTDYFMGISFSGREVPLYPGSEKIRLSYENKDEYVKAALNQRF-KEMMPQVLAVRKGLSALAPYPILKLLPSQTIESLVCGESSFPLWILKKIARYRDMDPSNEVIVWLWEILEEFNNDLKILFLIFVSGRSRLPTHAFDVSQ---------RFQILRVERPENGLPTAQTCFFQLRLPPYTSKEIMRNRLIYAIK 3331          
BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Match: EMLSAP00000000248 (pep:novel supercontig:LSalAtl2s:LSalAtl2s1036:68871:78712:-1 gene:EMLSAG00000000248 transcript:EMLSAT00000000248 description:"maker-LSalAtl2s1036-augustus-gene-0.34")

HSP 1 Score: 89.7373 bits (221), Expect = 2.583e-18
Identity = 66/282 (23.40%), Postives = 122/282 (43.26%), Query Frame = 0
Query:  659 DHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWIL--------SNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETP-----VVQWFWAIVDSYSSEMRARLLQFVTGSSRVPL--QGFKDLQGSTGQ-TGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVE 924
            + L+ F  VGR++G+ +               K +L + I   D+   DP +++SL+ ++        S  +   +D  FS++          EL  NG+++    +N   YV+ Y  +R     E+   +++ G  +++P   L     ++  L++ G+G I++    ++T       E+        +W W +V+  S   +  L+ F TGS  +P    GF+ +   T +  G  H            LP A TC +RL +P Y S  I+  KL  A++
Sbjct: 2723 ERLNAFRNVGRLMGLCLLQNELCPLFLNRHVIKYILGRTIRFHDLAFFDPVIYESLRQLVVDAENKETSAQLFSALDLNFSIDLSPEEGGGSVELIPNGREVEXXANNVYCYVRRYSYYRMIKCQEKALSNIKIGLYDVLPAGALDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGESQDRLVNFKRWLWQMVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSITIRPAGDSH------------LPSANTCISRLYVPLYSSKAILKSKLLLAIK 2992          
BLAST of EMLSAG00000006171 vs. SwissProt
Match: gi|73919319|sp|Q9V853.3|SMUF1_DROME (RecName: Full=E3 ubiquitin-protein ligase Smurf1; AltName: Full=HECT-type E3 ubiquitin transferase Smurf1; AltName: Full=Lethal with a checkpoint kinase protein; AltName: Full=SMAD ubiquitination regulatory factor 1 homolog; Short=DSmurf)

HSP 1 Score: 597.43 bits (1539), Expect = 0.000e+0
Identity = 274/392 (69.90%), Postives = 326/392 (83.16%), Query Frame = 0
Query:  542 GTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLT-NAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            G + LPK+++DLV K++ LR+ L   QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKF+ EEGLDYGGVAREWL+LLS EMLNP YGLFQYS +D YTLQINPDS VNPDHLSYFHFVGR +G+AVFHGH +DG FTTPFYK +LNKPI L DIE VDPDLH+SL W+L ++ISGII++TFSVE+ +FG   V+ELK  G  IPV E NK EYVKLYV +R+  GIEQQFL+LQKGF ELIP +LL+PFDE+ELELVIGGI +ID+ DW+N+TRLK C  ET  V WFW +V+SYSSEMRARLLQFVTGSSRVPLQGF+ LQGSTG  GPR FT+HLT + P  +LPKA TCFNR+DLP Y +++++ +KLTQAVEETCGFAVE
Sbjct:  670 GADLLPKYRRDLVGKLRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE 1061          

HSP 2 Score: 211.075 bits (536), Expect = 3.624e-55
Identity = 103/200 (51.50%), Postives = 140/200 (70.00%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVS----DTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNL-------TCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTT 195
            K+R+TILCARNLA++DLFRLPDPFA+V VD TG  + +E +K++ DPKWN HYDL L   ++ T++VWN +K+HK    SGFLGCVR+    IQ +K  G+QRLDL      D ++   V+GQI+ISLLS+DG  +G+  A+V   G++       +  + LPEGWEERRT +GRVYYVNH  ++TQW RP +P   G++
Sbjct:   14 KVRITILCARNLARKDLFRLPDPFAKVQVDGTGQVYSTEISKSSLDPKWNAHYDLFLGIGDAITITVWNQRKIHK---GSGFLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDEL---VRGQIIISLLSKDGPSSGNPLAIVGPSGDVRGPSEDDSSEDSLPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSS 207          

HSP 3 Score: 102.449 bits (254), Expect = 6.995e-21
Identity = 48/81 (59.26%), Postives = 61/81 (75.31%), Query Frame = 0
Query:  283 NLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRAL-TSNEELN-LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            +LPPGY M+TT+ GQ+Y+ H  TG +TWHDPR+ R   T +  L+ +GPLP GWEQR T S R YFV+HNN TTQF+DPR+
Sbjct:  514 DLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIPRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRL 594          
BLAST of EMLSAG00000006171 vs. SwissProt
Match: gi|218547424|sp|A9JRZ0.1|SMUF2_DANRE (RecName: Full=E3 ubiquitin-protein ligase SMURF2; AltName: Full=HECT-type E3 ubiquitin transferase SMURF2; AltName: Full=SMAD ubiquitination regulatory factor 2; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 2)

HSP 1 Score: 585.874 bits (1509), Expect = 0.000e+0
Identity = 309/629 (49.13%), Postives = 400/629 (63.59%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDAN-----NHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPS-----TSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSN------------ITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVP----NTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T S R  ++NH   +TQ+  P  P    ++     +   D+N+P     G +        +      S    +  S      P D P      T+ +  +    + +G ++      P  L+  N            I NT    V     NN           + + F    +S++  ++ +P P        + SSS P         +  P   P+DP  + +        PK+K+DLV K+K+LR  L+  QPQ G+C +EVSR+E+FE+SYRQ++KMR K + K+L VKFR EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS +D+YTLQINPDSAVNP+HLSYFHFVGRI+GMAVFHGH+IDG FT PFYK +L KPI LDD+E+VDPDLH SL WIL NDI+G++D+TF VEH A+G    +ELK NGK IPV +  K EYV+LYV +R+  GIE QFL+LQKGFNE+IPQ+LLK FDEKELEL++ G+G IDI DWK++TRLK C  ++ +V+WFW  V+SY  E RARLLQFVTGSSRVPLQGFK LQG+    GPR FT+H  +A  ++LPKA TCFNR+D+P Y S++ + +KL  A+EETCGFAVE
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRSTQWERPTRPASEYSSPGRPLSCLVDENTPIMTPNGAAGVPADDPRVQERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNVNCEELGPLPPGWEIRNTATGRVYFVDHNN-----------RTTQFTDPRLSANLHLVLNPSPNGSRAAVEAQSSSRPGQLKEQAQSVVSPGNLPEDPECLTV--------PKYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILDNDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTQDTKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIVCGLGKIDINDWKSNTRLKHCTPDSNIVKWFWRAVESYDEERRARLLQFVTGSSRVPLQGFKALQGA---AGPRLFTIHQIDASTNNLPKAHTCFNRIDIPPYESYDKLYDKLLTAIEETCGFAVE 765          

HSP 2 Score: 309.686 bits (792), Expect = 2.428e-91
Identity = 175/360 (48.61%), Postives = 220/360 (61.11%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG----LPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
             K+RLT+LCA+NL K+D FRLPDPFA+V VD +G  H ++  +NT DPKWN HYDL++  ++S T+SVWN KK+HKK   +GFLGCVRL+ N+I R+KDTGYQRLDL    K+G      V+GQIV+SL SRD  G+G    VVD   +    NDLP+GWEERRT SGR+ Y+NH+ R+TQW RPT PAS                 SS    LS      T   TP   GA  +P               P V  +   S     + S   R H  T PD LP GY  +TT+ GQ+Y++H  TG +TWHDPR+ R L++     LGPLPPGWE R T + R YFV+HNN TTQF+DPR+
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVRNTLDPKWNQHYDLYIGKSDSITISVWNHKKIHKKQ-GAGFLGCVRLLSNSINRLKDTGYQRLDL---NKLGPNDSDTVRGQIVVSLQSRDRIGSGG--PVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRSTQWERPTRPAS---------------EYSSPGRPLSCLVDENTPIMTPN--GAAGVPADD------------PRVQERRVRSQRHRNYMS---RTHLHTPPD-LPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNVNCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330          
BLAST of EMLSAG00000006171 vs. SwissProt
Match: gi|85681896|sp|Q9CUN6.2|SMUF1_MOUSE (RecName: Full=E3 ubiquitin-protein ligase SMURF1; AltName: Full=HECT-type E3 ubiquitin transferase SMURF1; AltName: Full=SMAD ubiquitination regulatory factor 1; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 1)

HSP 1 Score: 578.556 bits (1490), Expect = 0.000e+0
Identity = 269/406 (66.26%), Postives = 333/406 (82.02%), Query Frame = 0
Query:  530 PQDPLSIALHGG-GTEFLP--KFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            P  PL +   G    E LP  ++++DLV K+KVLR  L+  QPQ G+C +EVSR+E+FE+SYRQI+KMR K ++K+L VKFR EEGLDYGGVAREWLYLL HEMLNPYYGLFQYS++++YTLQINPDS++NPDHLSYFHFVGRI+G+AVFHGH+I+G FT PFYK +L KPI L D+E+VDP+LHKSL WIL NDI+ ++D+TF VEH AFG    +ELK NG+++PV E NK EYV+LYV +R+  GIE QFL+LQKGFNELIPQ+LLKPFD+KELEL+IGG+  ID+ DWK++TRLK C A++ +V+WFW  V+++  E RARLLQFVTGS+RVPLQGFK LQGSTG  GPR FT+HL +A  D+LPKA TCFNR+D+P Y S+E + EKL  AVEETCGFAVE
Sbjct:  326 PSQPLQLPSEGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE 731          

HSP 2 Score: 177.178 bits (448), Expect = 7.660e-45
Identity = 123/326 (37.73%), Postives = 164/326 (50.31%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLV----SDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNL---PPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEEL 325
            KIRLT+LCA+NLAK+D FRLPDPFA++ VD +G  H ++  KNT DPKWN HYDL++   +S T+SVWN KK+HKK   +GFLGCVRL+ NAI R+KDTGYQRLDL     SDT     V+GQIV+SL +RD  G G   +VVD  G L     + E     R  S           T          +C       +   L +    +  N        T  + P    +  LP       T  G   +  ++ Q   S+  DP     PR       D L   PPG+ +++T  G+IY++      T + DPRL   +    +L
Sbjct:   14 KIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQ-GAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTD---AVRGQIVVSLQTRDRIGGGG--SVVDCRGLLENEGTVYEDSGPGRPLS--CLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLV---QRLRNPEVRGPLQTPQNRPHGHQSPELPEGYEQRTTVQG--QVYFLHTQTGVSTWHDPRI---PRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQL 323          

HSP 3 Score: 108.612 bits (270), Expect = 5.896e-23
Identity = 59/131 (45.04%), Postives = 74/131 (56.49%), Query Frame = 0
Query:  267 PFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTN 397
            P  + Q R H    P+ LP GY  +TT  GQ+Y++H  TG +TWHDPR+ R L S     LGPLPPGWE R T S R YFV+HNN TTQF+DPR   ++   NH      P+ P +  S+       LP  
Sbjct:  220 PLQTPQNRPHGHQSPE-LPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPR---LHHIMNHQCQLKEPSQPLQLPSEGSVEDEELPAQ 346          
BLAST of EMLSAG00000006171 vs. SwissProt
Match: gi|123896339|sp|Q2TAS2.1|SMUF2_XENLA (RecName: Full=E3 ubiquitin-protein ligase SMURF2; AltName: Full=HECT-type E3 ubiquitin transferase SMURF2; AltName: Full=SMAD ubiquitination regulatory factor 2; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 2)

HSP 1 Score: 578.17 bits (1489), Expect = 0.000e+0
Identity = 305/628 (48.57%), Postives = 394/628 (62.74%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDAN-----NHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPS-----TSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSN------------ITNTTVSNVRATTSNNTVLR---PRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T S R  ++NH   TTQ+  P  P    ++     +   D+N+P   + G S  + +   +      S    +  S      P D P      T+ +  +    + +G ++      P  L   N            I NT    V     NN   +   PR      LS    L ++  +  +    PP        S   +P+    +                      +P++K+DLV K+K LR  L+  QPQ G+C +EVSR+E+FE+SYRQ++KMR K + K+L +KFR EEGLDYGGVAREWLYLLSH+MLNPYYGLFQYS +D+YTLQINPDSAVNP+HLSYFHFVGRI+GMAVFHGH+IDG FT PFYK +L KPI LDD+E+VDPDLH SL WIL NDI+G++D+TF VEH A+G    +ELK +GK IPV E  K EYV+LYV +R+  GIE QFL+LQKGFNE+IPQ+LLK FDEKELEL+I G+G ID+ DWK++TRLK C  ++ +V+WFW  V+S+  E RARLLQFVTGSSRVPLQGFK LQG+    GPR FT+H  +A  ++LPKA TCFNR+D+P Y ++E + EKL  A+EETCGFAVE
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGASCGQTSDPRISERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLGNVNCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPR------LSANLHLVLNRQNQQLKEQQPP-----QVVSLCQLPDEVECLT---------------------VPRYKRDLVHKLKSLRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHDMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKPITLDDMESVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGELIQHELKPSGKSIPVTEDTKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKAFDEKELELIICGLGKIDVSDWKSNTRLKHCTTDSNIVKWFWKAVESFDEERRARLLQFVTGSSRVPLQGFKALQGA---AGPRLFTIHQIDASTNNLPKAHTCFNRIDIPPYETYEKLYEKLLTAIEETCGFAVE 751          

HSP 2 Score: 307.375 bits (786), Expect = 1.234e-90
Identity = 174/360 (48.33%), Postives = 220/360 (61.11%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG----LPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
             K+RLT+LCA+NL K+D FRLPD FA+V VD +G  H ++  KNT DPKWN HYDL++  ++S T+SVWN KK+HKK   +GFLGCVRL+ NAI R+KDTGYQRLDL    K+G      V+GQIV+SL SRD  G+G    VVD   +    NDLP+GWEERRT SGR+ Y+NH+ RTTQW RPT PAS                 SS    LS      T             PI  ++  +   T + P ++ +   S     + S   R H  T PD LP GY  +TT+ GQ+Y++H  TG +TWHDPR+ R L +     LGPLPPGWE R T + R YFV+HNN TTQF+DPR+
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDSFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSITISVWNHKKIHKKQ-GAGFLGCVRLLSNAINRLKDTGYQRLDLC---KLGPNDNDTVRGQIVVSLQSRDRIGSGG--QVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPAS---------------EYSSPGRPLSCFVDENT-------------PITGTNGASCGQTSD-PRISERRVRSQRHRNYMS---RTHLHTPPD-LPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLGNVNCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330          
BLAST of EMLSAG00000006171 vs. SwissProt
Match: gi|17865624|sp|Q9HAU4.1|SMUF2_HUMAN (RecName: Full=E3 ubiquitin-protein ligase SMURF2; Short=hSMURF2; AltName: Full=HECT-type E3 ubiquitin transferase SMURF2; AltName: Full=SMAD ubiquitination regulatory factor 2; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 2)

HSP 1 Score: 577.4 bits (1487), Expect = 0.000e+0
Identity = 304/625 (48.64%), Postives = 393/625 (62.88%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDAN-----NHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPS-----TSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSN------------ITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T S R  ++NH   TTQ+  P  P    ++     +   D+N+P + + G +  + +   L      S    +  S      P D P      T+ +  +    + +G ++      P  L+  N            I NT    V     NN           + + F    +S++  ++ +       Q      S  P+    +                      +P++K+DLV K+K+LR  L+  QPQ G+C +EVSR+E+FE+SYRQ++KMR K + K+L +KFR EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS +D+YTLQINPDSAVNP+HLSYFHFVGRI+GMAVFHGH+IDG FT PFYK +L K I LDD+E VDPDLH SL WIL NDI+G++D+TF VEH A+G    +ELK NGK IPVNE NK EYV+LYV +R+  GIE QFL+LQKGFNE+IPQ+LLK FDEKELEL+I G+G ID+ DWK +TRLK C  ++ +V+WFW  V+ +  E RARLLQFVTGSSRVPLQGFK LQG+    GPR FT+H  +A  ++LPKA TCFNR+D+P Y S+E + EKL  A+EETCGFAVE
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNN-----------RTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLT---------------------VPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGA---AGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE 748          

HSP 2 Score: 311.997 bits (798), Expect = 2.246e-92
Identity = 180/360 (50.00%), Postives = 223/360 (61.94%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG----LPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
             K+RLT+LCA+NL K+D FRLPDPFA+V VD +G  H ++  KNT DPKWN HYDL++  ++S T+SVWN KK+HKK   +GFLGCVRL+ NAI R+KDTGYQRLDL    K+G      V+GQIV+SL SRD  GTG    VVD   +    NDLP+GWEERRT SGR+ Y+NH+ RTTQW RPT PAS                 SS    LS         +TP S G N        A  G  +D  P +  +   S     + S   R H  T PD LP GY  +TT+ GQ+Y++H  TG +TWHDPR+ R L++     LGPLPPGWE R T + R YFV+HNN TTQF+DPR+
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQ-GAGFLGCVRLLSNAINRLKDTGYQRLDLC---KLGPNDNDTVRGQIVVSLQSRDRIGTGG--QVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPAS---------------EYSSPGRPLS----CFVDENTPIS-GTNG-------ATCGQSSD--PRLAERRVRSQRHRNYMS---RTHLHTPPD-LPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330          
BLAST of EMLSAG00000006171 vs. SwissProt
Match: gi|17865625|sp|Q9HCE7.2|SMUF1_HUMAN (RecName: Full=E3 ubiquitin-protein ligase SMURF1; Short=hSMURF1; AltName: Full=HECT-type E3 ubiquitin transferase SMURF1; AltName: Full=SMAD ubiquitination regulatory factor 1; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 1)

HSP 1 Score: 576.63 bits (1485), Expect = 0.000e+0
Identity = 268/406 (66.01%), Postives = 332/406 (81.77%), Query Frame = 0
Query:  530 PQDPLSIALHGG-GTEFLP--KFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            P  PL +   G    E LP  ++++DLV K+KVLR  L+  QPQ G+C +EVSR+E+FE+SYRQI+KMR K ++K+L VKFR EEGLDYGGVAREWLYLL HEMLNPYYGLFQYS++++Y LQINPDS++NPDHLSYFHFVGRI+G+AVFHGH+I+G FT PFYK +L KPI L D+E+VDP+LHKSL WIL NDI+ ++D+TF VEH AFG    +ELK NG+++PV E NK EYV+LYV +R+  GIE QFL+LQKGFNELIPQ+LLKPFD+KELEL+IGG+  ID+ DWK++TRLK C A++ +V+WFW  V+++  E RARLLQFVTGS+RVPLQGFK LQGSTG  GPR FT+HL +A  D+LPKA TCFNR+D+P Y S+E + EKL  AVEETCGFAVE
Sbjct:  352 PSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE 757          

HSP 2 Score: 243.047 bits (619), Expect = 3.177e-67
Identity = 164/416 (39.42%), Postives = 208/416 (50.00%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLV----SDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRL--------------------------TRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTN----PSRGTSKNE 388
            KIRLT+LCA+NLAK+D FRLPDPFA++ VD +G  H ++  KNT DPKWN HYDL++   +S T+SVWN KK+HKK   +GFLGCVRL+ NAI R+KDTGYQRLDL     SDT     V+GQIV+SL +RD  GTG   +VVD  G L                 G VY         + + P  P SC                        +     T  +   + G N   + S    + +       + N D   SL  P    Q R H    P+ LP GY  +TT  GQ+Y++H  TG +TWHDPR+                           R L S     LGPLPPGWE R T S R YFV+HNN TTQF+DPR   ++   NH      P+     PS G+ ++E
Sbjct:   14 KIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQ-GAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTD---AVRGQIVVSLQTRDRIGTGG--SVVDCRGLL--------------ENEGTVY---------EDSGPGRPLSC----------------------FMEEPAPYTDSTGAAAGGGNCRFVESP---SQDQRLQAQRLRNPDVRGSLQTP----QNRPHGHQSPE-LPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPR---LHHIMNHQCQLKEPSQPLPLPSEGSLEDE 367          
BLAST of EMLSAG00000006171 vs. SwissProt
Match: gi|17865628|sp|Q9PUN2.1|SMUF1_XENLA (RecName: Full=E3 ubiquitin-protein ligase SMURF1; Short=xSMURF1; AltName: Full=HECT-type E3 ubiquitin transferase SMURF1; AltName: Full=SMAD ubiquitination regulatory factor 1; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 1)

HSP 1 Score: 574.704 bits (1480), Expect = 0.000e+0
Identity = 266/392 (67.86%), Postives = 324/392 (82.65%), Query Frame = 0
Query:  542 GTEF-LPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            G EF   ++++DLV K+KVLR  L+  QPQ G+C +EVSR+E+FE+SYRQI+KMR K ++K+L VKFR EEGLDYGGVAREWLYLL HEMLNPYYGLFQYS++++YTLQINPDS++NPDHLSYFHFVGRI+G+AVFHGH+I+G FT PFYK +L KPI L D+E+VDP+LHKSL WIL NDI+ ++D+TF VEH AFG    +ELK NGK++ V E NK EYV+LYV +R+  GIE QFL+LQKGFNELIPQ+LLKPF++KELEL+IGG+  IDI DWK +TRLK C A + +VQWFW  V+S+  E RARLLQFVTGS+RVPLQGFK LQGSTG  GPR FT+HL +A  D+LPKA TCFNR+D+P Y S+E + EKL  AVEET GFAVE
Sbjct:  340 GDEFPAQRYERDLVQKLKVLRHELSLLQPQAGHCRVEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITSVLDHTFCVEHNAFGRLLQHELKPNGKNLQVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFEQKELELIIGGLDKIDISDWKANTRLKHCLANSNIVQWFWQAVESFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETSGFAVE 731          

HSP 2 Score: 173.711 bits (439), Expect = 8.054e-44
Identity = 87/154 (56.49%), Postives = 110/154 (71.43%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVS-DTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPE 159
            ++RLT+LCA+NLAKRD FRLPDPFA++ VD +G  H ++  KNT DPKWN HYDL++   +S T+S+WN KK+HKK   +GFLGCVRL+ NAI R+KDTGYQRLDL   +      V+GQIV+SL +RD  GT  L +VVD  G L     L E
Sbjct:   14 RVRLTVLCAKNLAKRDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKMDSITISIWNHKKIHKKQ-GAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPTDNDAVRGQIVVSLQTRDRIGT--LGSVVDCRGLLDNEGALLE 164          

HSP 3 Score: 101.293 bits (251), Expect = 1.191e-20
Identity = 49/90 (54.44%), Postives = 60/90 (66.67%), Query Frame = 0
Query:  272 QPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            Q R H   Q  +LP GY  +TT  GQ+Y++H  TG +TWHDPR+ R L S    +LG LP GWE R T S R YFV+HNN TTQF+DPR+
Sbjct:  224 QNRSHGF-QSQDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNCDDLGSLPAGWEVRTTVSGRIYFVDHNNRTTQFTDPRL 312          
BLAST of EMLSAG00000006171 vs. SwissProt
Match: gi|218547425|sp|A2A5Z6.1|SMUF2_MOUSE (RecName: Full=E3 ubiquitin-protein ligase SMURF2; AltName: Full=HECT-type E3 ubiquitin transferase SMURF2; AltName: Full=SMAD ubiquitination regulatory factor 2; AltName: Full=SMAD-specific E3 ubiquitin-protein ligase 2)

HSP 1 Score: 574.318 bits (1479), Expect = 0.000e+0
Identity = 303/610 (49.67%), Postives = 389/610 (63.77%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDAN-----NHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPV--SSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T S R  ++NH   TTQ+  P  P    ++     +   D+N+P   + G +    +   L      S    +  S      P D P    +            T T  S+      P +++N     +           P G  I+  +   V  V  ++ ++  + P          +  + + +       P C P D   + +        P++K+DLV K+K+LR  L+  QPQ G+C +EVSR+E+FE+SYRQ++KMR K + K+L +KFR EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS +D+YTLQINPDSAVNP+HLSYFHFVGRI+GMAVFHGH+IDG FT PFYK +L K I LDD+E VDPDLH SL WIL NDI+G++D+TF VEH A+G    +ELK NGK IPV E NK EYV+LYV +R+  GIE QFL+LQKGFNE+IPQ+LLK FDEKELEL+I G+G ID+ DWK +TRLK C  ++ VV+WFW  V+ +  E RARLLQFVTGSSRVPLQGFK LQG+    GPR FT+H  +A  ++LPKA TCFNR+D+P Y S+E + EKL  A+EETCGFAVE
Sbjct:  159 LPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGATCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPL--------PPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVPLC-PDDTECLTV--------PRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGA---AGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE 748          

HSP 2 Score: 310.842 bits (795), Expect = 6.384e-92
Identity = 179/360 (49.72%), Postives = 224/360 (62.22%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG----LPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
             K+RLT+LCA+NL K+D FRLPDPFA+V VD +G  H ++  KNT DPKWN HYDL++  ++S T+SVWN KK+HKK   +GFLGCVRL+ NAI R+KDTGYQRLDL    K+G      V+GQIV+SL SRD  GTG    VVD   +    NDLP+GWEERRT SGR+ Y+NH+ RTTQW RPT PAS                 SS    LS         +TP + G N        A  G+ +D  P +  +   S     + S   R H  T PD LP GY  +TT+ GQ+Y++H  TG +TWHDPR+ R L++     LGPLPPGWE R T + R YFV+HNN TTQF+DPR+
Sbjct:   12 VKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQ-GAGFLGCVRLLSNAINRLKDTGYQRLDLC---KLGPNDNDTVRGQIVVSLQSRDRIGTGG--QVVDC--SRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPAS---------------EYSSPGRPLS----CFVDENTPIT-GTNG-------ATCGHSSD--PRLAERRVRSQRHRNYMS---RTHLHTPPD-LPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRL 330          
BLAST of EMLSAG00000006171 vs. SwissProt
Match: gi|74598276|sp|Q5BDP1.1|RSP5_EMENI (RecName: Full=E3 ubiquitin-protein ligase RSP5; AltName: Full=HECT ubiquitin ligase A; AltName: Full=HECT-type E3 ubiquitin transferase RSP5)

HSP 1 Score: 436.802 bits (1122), Expect = 1.386e-138
Identity = 253/627 (40.35%), Postives = 353/627 (56.30%), Query Frame = 0
Query:  321 SNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTN--PSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSN----VRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVI-----GGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQP----QNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            S+ E N G LP GWE+R  +  R Y+V+HN  TT ++ P       AN +     +    +    +      +LP +   + SP  ++T    + + PA     SN   +S+  + + +  T        L P     TT       V   T   T + PR    QQ         S  ++ I   P        S     + NT  V           W    L  +L  G    +P++K+D   K+   RS     QP     +G C ++V R  +FEDSY +I++     ++K+L +KF  E+GLDYGG++RE+ +LLSHEM NP+Y LF+YS+ D YTLQINP S VNP+HL+YF F+GR++G+A+FH  F+D  F   FYKMML K ++L D+E VD DLH++L W L NDI GIID TF+V+ E FG  +  ELK  G+DIPV   NK EYV+L  +++    +E+QF +   GFNELIP +L+  FDE+ELEL+IGGI  ID+ DWK HT  +    +  V+Q FW IV ++ +E ++RLLQF TG+SR+P+ GFKDLQGS    GPR FT+  +  P+ +LPK+ TCFNRLDLP Y S E++  KL+ AVEET GF  E
Sbjct:  224 SSFEDNQGRLPAGWERREDNLGRTYYVDHNTRTTTWNRP------SANYNEQTQRTQREANMQLERRAHQNRMLPEDRTGASSPNLSETQPQAQ-TPPAGGSGASNSNVVSMMATGATTAGT------GELPPGWEQRTTPEGRPYFVDHNTRTTTWVDPRR---QQYIRMYGQNASGGNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQG----VPQYKRDFRRKLIYFRS-----QPALRIMSGQCHVKVRRNNIFEDSYAEIMRQSASDLKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGVNPEHLNYFKFIGRVVGLAIFHRRFLDSFFIGAFYKMMLRKKVSLQDMEGVDEDLHRNLTWTLENDIEGIIDLTFTVDDEKFGERRTIELKPGGEDIPVTNENKHEYVELVTEWKIVKRVEEQFNAFMSGFNELIPADLVNVFDERELELLIGGIADIDVDDWKKHTDYRGYQEQDEVIQNFWKIVRTWDAEQKSRLLQFTTGTSRIPVNGFKDLQGS---DGPRRFTIEKSGDPI-ALPKSHTCFNRLDLPPYKSHEVLEHKLSIAVEETLGFGQE 821          

HSP 2 Score: 100.138 bits (248), Expect = 2.787e-20
Identity = 71/220 (32.27%), Postives = 97/220 (44.09%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPR---LTRALTSNEE-----------LNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIP 362
            LP GWE R    GR YYV+H  RTT W RP+   +   T+R  R   + +   +  N +       T  S+P    + + P A +    G+G  N   V              S+            LPPG+  +TT  G+ Y++   T  TTW DPR     R    N               LGPLP GWE R+T+++R YFV+HN  TT + DPR+P
Sbjct:  233 LPAGWERREDNLGRTYYVDHNTRTTTWNRPSANYN-EQTQRTQREANMQLERRAHQNRM--LPEDRTGASSPNL--SETQPQAQTPPAGGSGASNSNVV--------------SMMATGATTAGTGELPPGWEQRTTPEGRPYFVDHNTRTTTWVDPRRQQYIRMYGQNASGGNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLP 433          

HSP 3 Score: 63.929 bits (154), Expect = 4.016e-9
Identity = 41/124 (33.06%), Postives = 65/124 (52.42%), Query Frame = 0
Query:    8 IRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDL-VSDTKIGLPVKGQIVISL 130
            +R+TI+ A  L KRD+FRLPDPFA   V     TH +   K T +P WN  +DL +  NE   L++    +   K  + GFLG + +    +  ++  G + L   +  +   L V G+++I+L
Sbjct:   11 LRVTIIAADGLYKRDVFRLPDPFAVATVGGEQ-THTTSVIKKTLNPYWNEMFDLRV--NEDSILAIQIFDQKKFKKKDQGFLGVINVRIGDVIDLQMGGDEMLTRDLKKSNDNLVVHGKLIINL 131          
BLAST of EMLSAG00000006171 vs. SwissProt
Match: gi|300681225|sp|Q4WTF3.2|RSP5_ASPFU (RecName: Full=Probable E3 ubiquitin-protein ligase hulA; AltName: Full=HECT ubiquitin ligase A; AltName: Full=HECT-type E3 ubiquitin transferase hulA >gi|300681228|sp|B0XQ72.2|RSP5_ASPFC RecName: Full=Probable E3 ubiquitin-protein ligase hulA; AltName: Full=HECT ubiquitin ligase A; AltName: Full=HECT-type E3 ubiquitin transferase hulA)

HSP 1 Score: 434.491 bits (1116), Expect = 1.030e-137
Identity = 248/626 (39.62%), Postives = 354/626 (56.55%), Query Frame = 0
Query:  316 TRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVI-----GGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQP----QNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            +R   S+ E + G LP GWE+R  +  R Y+V+HN  TT ++ P       +N + H   S    +    +    + +LP + + +   N   S    S+ A +P     A  +V+  ++G+T+  T   P               V   T   T + PR    QQ         + +++ I   P        S     + NT  V           W    L  +L  G    +P++K+D   K+   RS     QP     +G C ++V R  +FEDSY +I++     ++K+L +KF  E+GLDYGG++RE+ +LLSHEM NP+Y LF+YS+ D YTLQINP S VNP+HL+YF F+GR++G+A+FH  F+D  F   FYKMML K ++L D+E VD DLH++L W L NDI G+++ TFSV+ E FG  +  +LK  G+DIPV   NK EYV+L  +++    +E+QF +   GFNELIP +L+  FDE+ELEL+IGGI  ID+ DWK HT  +       V+Q FW IV S+ +E ++RLLQF TG+SR+P+ GFKDLQGS    GPR FT+  +  P  +LPK+ TCFNRLDLP Y S+E +  K++ AVEET GF  E
Sbjct:  215 SRTNLSSFEDSQGRLPAGWERREDNLGRTYYVDHNTRTTTWTRP------SSNYNEHAQRSQREANMQLERRAHQSRMLPEDRTGANSPNLPES----SQQAHTPPAGGSAN-AVSMMATGATTAGTGELPPGWEQRTTPEGRPYFVDHNTRTTTWVDPRR---QQYIRMYGQNANGTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQG----VPQYKRDFRRKLIYFRS-----QPALRIMSGQCHVKVRRNNIFEDSYAEIMRQSASDLKKRLMIKFDGEDGLDYGGLSREFFFLLSHEMFNPFYCLFEYSAHDNYTLQINPHSGVNPEHLNYFKFIGRVVGLAIFHRRFLDSFFIGAFYKMMLRKKVSLQDMEGVDEDLHRNLTWTLDNDIEGVLELTFSVDDEKFGERRTIDLKPGGRDIPVTNENKAEYVELVTEWKIVKRVEEQFNAFMSGFNELIPADLVNVFDERELELLIGGIADIDVDDWKKHTDYRGYQESDEVIQNFWKIVRSWDAEQKSRLLQFTTGTSRIPVNGFKDLQGS---DGPRRFTIEKSGDPA-ALPKSHTCFNRLDLPPYKSYETLEHKMSIAVEETLGFGQE 813          

HSP 2 Score: 98.9821 bits (245), Expect = 7.675e-20
Identity = 71/222 (31.98%), Postives = 99/222 (44.59%), Query Frame = 0
Query:  157 LPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANS--LPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALT--------SNEEL------NLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIP 362
            LP GWE R    GR YYV+H  RTT W RP+   +    +R  R   + +   +  + +     T          GANS  LP +S  A T               P+  S    S+            LPPG+  +TT  G+ Y++   T  TTW DPR  + +         +N  +       LGPLP GWE R+T+++R YFV+HN  TT + DPR+P
Sbjct:  229 LPAGWERREDNLGRTYYVDHNTRTTTWTRPSSNYN-EHAQRSQREANMQLERRAHQSRMLPEDRT----------GANSPNLPESSQQAHT--------------PPAGGSANAVSMMATGATTAGTGELPPGWEQRTTPEGRPYFVDHNTRTTTWVDPRRQQYIRMYGQNANGTNTTIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLP 425          
BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Match: EEB15624.1 (E3 ubiquitin-protein ligase Smurf1, putative [Pediculus humanus corporis])

HSP 1 Score: 781.556 bits (2017), Expect = 0.000e+0
Identity = 437/940 (46.49%), Postives = 578/940 (61.49%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLV-SDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSP-----ETNDTHS--------NVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            K+RLTILCARNLA++ LFRLPDPFA++ VD +G  H +E  + T DPKWN HYDL++   +  T+SVWN KK+HKK +  GFLGCVR+  +A+QR++DTGYQ L+L  +      PVKGQIV+SLLSRDG  +G+  AVVD+ G+++   DLP GWEER+T +GR+YYVNH  +TTQW RP           GD G T+ + ++  ++N   + +++       ++  NS  +A+  +L   G   L   ++  + S+ ++ FS     R + T+  N   G    T+   QI      +       P+ +             LP G+E R T   + YF +  + T+ + DPRIP                             ++ P+   L P     E   THS        N R ++  D   TS+     +   +    ST +     S    N  N   S  +   +N                   LP S+S            P+ ++S    V  +   I   +   +DP+   L G   E LPK+K+DLV+K+++L++ L+  QPQ+G+C LEVSR+E+FE+SYR ++KMR K +RK+L VKFR EEGLDYGGVAREWLYL+S EMLNP YGLFQYS ED YTLQIN DS VNP+HLSYFHF GR++G A+FHGH ID  FTTPFYKM+L+K I LDDI  VDPDLH+SL+W+L ND++ + D TF+VEHEAFG  +V+ELK  G+DIPV + NK EYVKLYV +R+  GIEQQFL+L KGF E++P NLL+ FDE+ELELV+GGI  ID+ DWK +TRLK C   TPVV WFW IV+ YS EM+ARLLQFVTGSSRVPLQGF+ LQG+TG  GPR FT+HL +AP+ +LPKA TCFNR+++P YP  E M EKLTQAVEETCGFAVE
Sbjct:   14 KVRLTILCARNLARKGLFRLPDPFAKISVDGSGQCHSTEVCRATLDPKWNQHYDLYIGKCDGITISVWNHKKIHKK-HGGGFLGCVRIAASAVQRLRDTGYQCLELCKAKADDPEPVKGQIVVSLLSRDG-VSGNRTAVVDSSGHISNPEDLPPGWEERKTLAGRLYYVNHNTKTTQWIRPNG--------EGD-GDTMQLPDNVNSSNDENSCSSSAQFFRSQNQSLNS-SLANCSSLPC-GESGLITSSDLKNSSNNTEVFSC----RRKHTRIKNAETG----TSNELQI----NSSKNNKQQKPQKSV-----------DLPHGFEMRTTQQGQVYFYHVPSGTSTWHDPRIP----------------------------RDLFPSEAELGPLPPGWEARKTHSGRTYFVDHNSRTTQFNDPRLTSSLINKFLKNRNQNVPSTASGEESVSFESRNNGNCITSEAQNLKNN-------------------LPESNSF-----------PEASTSGIERVAFSDSGIADNKVKTEDPV---LDG---EILPKYKRDLVSKLRMLKAELHALQPQSGHCRLEVSRKEIFEESYRLLMKMRPKDLRKRLIVKFRGEEGLDYGGVAREWLYLVSREMLNPQYGLFQYSREDNYTLQINADSGVNPEHLSYFHFAGRVLGTAMFHGHHIDAGFTTPFYKMLLSKSITLDDIAGVDPDLHRSLQWLLHNDVTQL-DTTFAVEHEAFGELRVHELKVGGRDIPVTQENKKEYVKLYVNYRFKRGIEQQFLALLKGFTEVVPANLLRLFDERELELVLGGISKIDVEDWKQNTRLKHCTLTTPVVVWFWEIVEKYSDEMQARLLQFVTGSSRVPLQGFRALQGTTGAAGPRLFTIHLIDAPIHNLPKAHTCFNRIEIPPYPYKEYMYEKLTQAVEETCGFAVE 852          
BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Match: XP_006564410.1 (PREDICTED: E3 ubiquitin-protein ligase SMURF2 isoform X2 [Apis mellifera])

HSP 1 Score: 619.002 bits (1595), Expect = 0.000e+0
Identity = 286/390 (73.33%), Postives = 330/390 (84.62%), Query Frame = 0
Query:  544 EFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLP-AYPSFEIMAEKLTQAVEETCGFAVE 932
            E LPK+K+DLVAK+K LR+ LN  QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKFR EEGLDYGGVAREWLYLLSHEMLNP YGLFQYS +D YTLQINPDS +NP+HLSYFHF GRIIG+AVFHGH IDG FTTPFYKM+LNK I L DIE VDP+LH+SL W+L N I G++D TFSVEH +FG+ K +ELK  GKDIPV E NK EYV+LYV +R+  GIEQQFL+LQKGF+ELIP  LL+PFDE+ELELVIGG+GTIDI DWK HTRLK C  +TPVV+WFW IV+SY  EMRARLLQFVTGSSRVPLQGFK LQGSTG  GPR FT+H  +AP ++LPKA TCFNR+D+P +YPS++ M +KLTQAVEETCGFAVE
Sbjct:  509 ELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLTDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIPVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPQLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHAVDAPSENLPKAHTCFNRIDIPESYPSYQKMLDKLTQAVEETCGFAVE 898          

HSP 2 Score: 312.383 bits (799), Expect = 1.978e-91
Identity = 204/494 (41.30%), Postives = 256/494 (51.82%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVS----DTKIGLPVKGQIVISLLSRDGH-----GTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPT-------------------------------------EPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTT------------TTXXSTPTSEGANSLPIASSHALTGNGTDNL---PAVNNQDSPSSLSDPFSSL-----QPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELN-LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIP---LMNDANNHHHDDNSPT-NPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRN--SEPADSPSTSNEAP 427
            KIRLTILCARNLA++DLFRLPDPFA++ VD +G  H +   K T DP WN HYDL++  ++  T+SVWN KK+HKK     FLGCVR++ N IQR+KDTGYQRLDL      DT +   VKG++V+SLLSRDGH      T   NAVVD LG+L+C  DLP+GWEERRT SGR+YYVNH  RTTQW RP                                       P  C     GD   + + +  S   N  + T T             T   TP S      PI +S    GN   N+    ++NN           +         RR+   QP +LP GY M+ T+ GQ+Y+ H  TG +TWHDPR+ R L +NE  N LGPLP GWE R T S R YFV+HNN TTQF+DPR+    + N  N   +  N  T N +   + NE      P +PS+        S   N     A +  T +E P
Sbjct:   14 KIRLTILCARNLARKDLFRLPDPFAKITVDGSGQCHSTHTCKATLDPMWNQHYDLYIGKDDGITISVWNHKKIHKKKGGG-FLGCVRILSNTIQRLKDTGYQRLDLCKAHSDDTDV---VKGEVVVSLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTRSGRLYYVNHYTRTTQWIRPNARPTNGIIPTTPEPPPLPSSEPTSPSGSGSPPNLRTESPTRCTPEWNGDGTPSRTPSRDSSTPNTPRNTPTQQQNRNTQQNQHNTRNVTPAS-SRERRPIRASDEQNGNQNGNVRSDRSINNAQPRRPNRSNRNRNSVMINNERRYDPPQPPDLPRGYEMRKTQQGQVYFYHVPTGSSTWHDPRIPRDLPANELANELGPLPSGWEMRQTQSGRVYFVDHNNRTTQFTDPRLSSQIISNLLNRRQNSTNQTTSNSNNSATINESTEIETPASPSIQCNIVQQRSRTENGMENTAQNAQTVSELP 502          
BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Match: EFX80702.1 (hypothetical protein DAPPUDRAFT_318414 [Daphnia pulex])

HSP 1 Score: 612.453 bits (1578), Expect = 0.000e+0
Identity = 291/436 (66.74%), Postives = 349/436 (80.05%), Query Frame = 0
Query:  498 SSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIAL-HGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            S P P  PP    ++S +     PV       P  P++  L      E LP++K+DLVAKIK+LR+ L   QPQ+G+C LEVSR E+FE+SYR I+K+R K +RK+L VKFR EEGLDYGGVAREWL+LLS +MLNPYYGLF YS ED YTL +NPDS+VNP+HLSYFHFVGRI+G+AVFHGH +DG FT PFYKM+LNK I LDDI  VDP+LH+SL W+L +DI+G+ID TFSVEH A+G  +V++LK +G+D+ V+E NK EYV+LYV +R+  GIEQQFLSLQKGF+ELIP NLL+PFDE+ELEL+IGGI  ID  DW+ +TRLK C AETPVVQWFW IV+SYS E+RARLLQFVTGSSRVPLQGFK LQGSTG +GPR FT+HL +AP ++LPKA TCFNRLDLP YPS+E M EKLTQAVEETCGFAVE
Sbjct:  372 SQPQPTLPPPNAVATSENGACAAPVANSTSAPP--PMATPLPDEKQEEALPRYKRDLVAKIKILRTELQTMQPQSGHCRLEVSRTEIFEESYRSIMKLRPKDLRKRLMVKFRGEEGLDYGGVAREWLHLLSQQMLNPYYGLFCYSREDNYTLHVNPDSSVNPEHLSYFHFVGRIMGLAVFHGHHLDGGFTLPFYKMLLNKAIVLDDITHVDPELHRSLTWMLESDITGVIDTTFSVEHNAYGAIQVHDLKPDGRDLIVSEENKREYVRLYVSYRFMRGIEQQFLSLQKGFHELIPANLLRPFDERELELLIGGICKIDTADWQANTRLKHCTAETPVVQWFWQIVESYSEELRARLLQFVTGSSRVPLQGFKALQGSTGASGPRLFTIHLIDAPCENLPKAHTCFNRLDLPPYPSYEKMHEKLTQAVEETCGFAVE 805          

HSP 2 Score: 93.5893 bits (231), Expect = 1.497e-18
Identity = 51/105 (48.57%), Postives = 66/105 (62.86%), Query Frame = 0
Query:  282 DNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNL-GPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR----IPLMNDANNHHHDDN-SPTNP 380
            ++LP GY M+ T  GQ+Y+ H  TG +TW+DPR+ R L    +L + G LPPGWE R T S R YFV+HNN TTQF+DPR    + L N  N H  D   +P+ P
Sbjct:  273 NDLPDGYEMRKTGQGQVYFYHVTTGISTWYDPRIPRDL---RDLPMPGALPPGWEMRHTPSGRIYFVDHNNRTTQFTDPRLSTGLALQNLLNLHRSDHRAAPSQP 374          
BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Match: gb|KFM66753.1| (E3 ubiquitin-protein ligase SMURF2, partial [Stegodyphus mimosarum])

HSP 1 Score: 610.142 bits (1572), Expect = 0.000e+0
Identity = 304/496 (61.29%), Postives = 372/496 (75.00%), Query Frame = 0
Query:  437 STSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            +T  T     SSLA  N+ NT  ++  +   N  +L  R              V  SS  +SS    P   T S+S+  +   PP I      P  PL I+     T +  K ++DLV K+  LR  L+  QPQ+G+C LEVSR+++FE+SYR I+KMR K +RK+L VKFR+EEGLDYGGVAREWLYLLSHEMLNPYYGLFQY+ +D+YTLQIN DS+VNP+HLSYFHFVGR+IG+AVFHGH+IDG FT PFYKM+LNKPI LDDIEAVDP+L +SLKW+L N+I+ +ID +FSVEH+AFG  +V+ELK NG++IPV + NK EYV+LYV +R+  GIEQQFL+LQKGFNELIPQ+LLK FDEKELELVIGG+G ID+ DWK +TRLK C+ +T +V+WFW  VDSYS E RARLLQFVTGSSRVPLQGFK LQGSTG  GPR FT+HL +A  D+LPKA TCFNR+D+P Y S+E + EKLTQA+EETCGFAVE
Sbjct:  126 TTQFTDPRLSSSLAVQNMLNTRRNS--SIEDNTNILNGR--------------VPDSSQAVSSVNHTPSAITPSNSAPPLHPLPPTIN-----PAVPLEIS----DTPY-SKQRRDLVQKLAALRQELHALQPQSGHCRLEVSREDIFEESYRLIMKMRPKDLRKRLMVKFRSEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYTRDDIYTLQINHDSSVNPEHLSYFHFVGRVIGLAVFHGHYIDGGFTLPFYKMLLNKPITLDDIEAVDPELQRSLKWMLENNITSVIDTSFSVEHDAFGQIQVHELKPNGREIPVTDENKREYVRLYVNYRFMRGIEQQFLALQKGFNELIPQHLLKSFDEKELELVIGGLGKIDLEDWKMNTRLKHCNPDTNIVKWFWKAVDSYSEEQRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHLIDASTDNLPKAHTCFNRIDIPPYESYEKLYEKLTQAIEETCGFAVE 595          

HSP 2 Score: 105.145 bits (261), Expect = 2.224e-22
Identity = 66/189 (34.92%), Postives = 94/189 (49.74%), Query Frame = 0
Query:  275 RHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVR 463
            R+ + Q   LP GY ++TT+ GQ+Y+ H  TG +TWHDPR+ R L      +LGPLP GWE R T + R Y+V+HNN TTQF+DPR+                       S +    N+L T  + S E N    N R  + + + S+ N  P ++T S+S         T +   P  I++T  S  R
Sbjct:   48 RNHLHQAVELPDGYEIRTTQQGQVYFYHVSTGVSTWHDPRVPRDLAGINVGDLGPLPNGWEMRHTANGRVYYVDHNNRTTQFTDPRL-----------------------SSSLAVQNMLNTRRNSSIEDNTNILNGRVPDSSQAVSSVNHTPSAITPSNSAPPLHPLPPTINPAVPLEISDTPYSKQR 213          
BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Match: AGB93593.1 (SMAD specific E3 ubiquitin protein ligase, isoform B [Drosophila melanogaster])

HSP 1 Score: 597.43 bits (1539), Expect = 0.000e+0
Identity = 274/392 (69.90%), Postives = 326/392 (83.16%), Query Frame = 0
Query:  542 GTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLT-NAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            G + LPK+++DLV K++ LR+ L   QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKF+ EEGLDYGGVAREWL+LLS EMLNP YGLFQYS +D YTLQINPDS VNPDHLSYFHFVGR +G+AVFHGH +DG FTTPFYK +LNKPI L DIE VDPDLH+SL W+L ++ISGII++TFSVE+ +FG   V+ELK  G  IPV E NK EYVKLYV +R+  GIEQQFL+LQKGF ELIP +LL+PFDE+ELELVIGGI +ID+ DW+N+TRLK C  ET  V WFW +V+SYSSEMRARLLQFVTGSSRVPLQGF+ LQGSTG  GPR FT+HLT + P  +LPKA TCFNR+DLP Y +++++ +KLTQAVEETCGFAVE
Sbjct:  670 GADLLPKYRRDLVGKLRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE 1061          

HSP 2 Score: 211.075 bits (536), Expect = 1.756e-55
Identity = 103/200 (51.50%), Postives = 140/200 (70.00%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVS----DTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNL-------TCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTT 195
            K+R+TILCARNLA++DLFRLPDPFA+V VD TG  + +E +K++ DPKWN HYDL L   ++ T++VWN +K+HK    SGFLGCVR+    IQ +K  G+QRLDL      D ++   V+GQI+ISLLS+DG  +G+  A+V   G++       +  + LPEGWEERRT +GRVYYVNH  ++TQW RP +P   G++
Sbjct:   14 KVRITILCARNLARKDLFRLPDPFAKVQVDGTGQVYSTEISKSSLDPKWNAHYDLFLGIGDAITITVWNQRKIHK---GSGFLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDEL---VRGQIIISLLSKDGPSSGNPLAIVGPSGDVRGPSEDDSSEDSLPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSS 207          

HSP 3 Score: 102.449 bits (254), Expect = 3.390e-21
Identity = 48/81 (59.26%), Postives = 61/81 (75.31%), Query Frame = 0
Query:  283 NLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRAL-TSNEELN-LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            +LPPGY M+TT+ GQ+Y+ H  TG +TWHDPR+ R   T +  L+ +GPLP GWEQR T S R YFV+HNN TTQF+DPR+
Sbjct:  514 DLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIPRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRL 594          
BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Match: AAF57824.3 (SMAD specific E3 ubiquitin protein ligase, isoform A [Drosophila melanogaster])

HSP 1 Score: 597.43 bits (1539), Expect = 0.000e+0
Identity = 274/392 (69.90%), Postives = 326/392 (83.16%), Query Frame = 0
Query:  542 GTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLT-NAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            G + LPK+++DLV K++ LR+ L   QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKF+ EEGLDYGGVAREWL+LLS EMLNP YGLFQYS +D YTLQINPDS VNPDHLSYFHFVGR +G+AVFHGH +DG FTTPFYK +LNKPI L DIE VDPDLH+SL W+L ++ISGII++TFSVE+ +FG   V+ELK  G  IPV E NK EYVKLYV +R+  GIEQQFL+LQKGF ELIP +LL+PFDE+ELELVIGGI +ID+ DW+N+TRLK C  ET  V WFW +V+SYSSEMRARLLQFVTGSSRVPLQGF+ LQGSTG  GPR FT+HLT + P  +LPKA TCFNR+DLP Y +++++ +KLTQAVEETCGFAVE
Sbjct:  670 GADLLPKYRRDLVGKLRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE 1061          

HSP 2 Score: 211.075 bits (536), Expect = 1.756e-55
Identity = 103/200 (51.50%), Postives = 140/200 (70.00%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVS----DTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNL-------TCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTT 195
            K+R+TILCARNLA++DLFRLPDPFA+V VD TG  + +E +K++ DPKWN HYDL L   ++ T++VWN +K+HK    SGFLGCVR+    IQ +K  G+QRLDL      D ++   V+GQI+ISLLS+DG  +G+  A+V   G++       +  + LPEGWEERRT +GRVYYVNH  ++TQW RP +P   G++
Sbjct:   14 KVRITILCARNLARKDLFRLPDPFAKVQVDGTGQVYSTEISKSSLDPKWNAHYDLFLGIGDAITITVWNQRKIHK---GSGFLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDEL---VRGQIIISLLSKDGPSSGNPLAIVGPSGDVRGPSEDDSSEDSLPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSS 207          

HSP 3 Score: 102.449 bits (254), Expect = 3.390e-21
Identity = 48/81 (59.26%), Postives = 61/81 (75.31%), Query Frame = 0
Query:  283 NLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRAL-TSNEELN-LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            +LPPGY M+TT+ GQ+Y+ H  TG +TWHDPR+ R   T +  L+ +GPLP GWEQR T S R YFV+HNN TTQF+DPR+
Sbjct:  514 DLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIPRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRL 594          
BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Match: gb|EFA08997.2| (E3 ubiquitin-protein ligase Smurf1-like Protein [Tribolium castaneum])

HSP 1 Score: 597.045 bits (1538), Expect = 0.000e+0
Identity = 275/389 (70.69%), Postives = 326/389 (83.80%), Query Frame = 0
Query:  544 EFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            + LPK+++DLVAK+K LR+ L   QPQ+G+C LEVSR EVFE+SYR I+KMR K MRK+L VKF+ EEGLDYGGVAREWL+LLS EMLNP YGLFQYS +D YTLQINPDSAVNP+HLSYFHFVGRI+G+AVFH H ++G FT PFYK +LNKPI L DIE VDP+LH+SL W+L N+I G++D TFSVE+ +FG+ KV+ELK +G  IPV E NK EYVKLYV +R+  GIEQQFL+LQKGF ELIP +LL+PFDE+ELELVI GIG+IDI DW++HTRLK C  ETPVVQWFW +V+SYS EMRARLLQFVTGSSRVPLQGFK LQGSTG  GPR FT+H  +    +LPKA TCFNR+D+P Y S++ +A+KLTQAVEETCGFAVE
Sbjct:  526 DHLPKYRRDLVAKLKTLRAELTALQPQSGHCRLEVSRNEVFEESYRLIMKMRPKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSAVNPEHLSYFHFVGRILGIAVFHNHQLEGGFTLPFYKQLLNKPITLQDIEGVDPELHRSLTWMLENNIDGVLDTTFSVENNSFGIVKVHELKPSGATIPVTEDNKREYVKLYVNYRFMRGIEQQFLALQKGFTELIPPSLLRPFDERELELVISGIGSIDIADWRSHTRLKHCTPETPVVQWFWQVVESYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTIHCIDCSPQNLPKAHTCFNRIDIPPYDSYQTLADKLTQAVEETCGFAVE 914          

HSP 2 Score: 222.631 bits (566), Expect = 1.176e-59
Identity = 149/396 (37.63%), Postives = 213/396 (53.79%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLV----SDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNL----------TCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRR------------------GDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSH-----ALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRA---LTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIP 362
            KIRLTILCARNL ++DLFRLPDPFA++ VD +G  H +E  KNT +PKWN+HYDL++  N+S T+SVWN +K+ KK   SGFLGCVR++ + IQR++DTGYQ LDL     +D++   PVKGQI+ISLLSRDG  +G+  AVV   G L          + ++DLP GWEERRT +GR YYVNH+ R+TQW +   P S   TRR                  G + G   I N++    L+ +++T+      + +   S P  + +      L+   T  +PA NN    +   +  + + P+  Q      +      + +   +              +   T A   +T N +L+   LPPG+E R T   + YF +     + + DPRIP
Sbjct:   14 KIRLTILCARNLVRKDLFRLPDPFAKISVDGSGQCHSTEFVKNTLEPKWNSHYDLYIGRNDSITISVWNHRKVIKKQ-GSGFLGCVRIVNSHIQRLRDTGYQCLDLGKACPADSE---PVKGQIIISLLSRDGPCSGTPLAVVGPQGELRGPTDRDSSPSNNDDLPPGWEERRTENGRPYYVNHITRSTQWIK---PQSTNKTRRRSTHQNDNNNTNNENTIGGTQMGVQEILNNNTGETLTPSSSTSPVSPVVSPQKTTSTPQTTDNRNNFITLSPTSTSVIPAPNNISCNAPNGENTNVVSPQMGQGQGQRAIVRSERRQRSAEERRDGSSRRRSGRNNRNSGGTTAQGPVTQNSKLD---LPPGYELRTTQQGQVYFYHIPTGVSTWHDPRIP 399          

HSP 3 Score: 102.449 bits (254), Expect = 2.935e-21
Identity = 47/81 (58.02%), Postives = 60/81 (74.07%), Query Frame = 0
Query:  283 NLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALT--SNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            +LPPGY ++TT+ GQ+Y+ H  TG +TWHDPR+ + L   S    +LGPLPPGWE R T S R YFV+HNN TTQF+DPR+
Sbjct:  366 DLPPGYELRTTQQGQVYFYHIPTGVSTWHDPRIPKDLAPLSLALDHLGPLPPGWEMRQTASGRIYFVDHNNRTTQFTDPRL 446          
BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Match: EAA05201.4 (AGAP011242-PA [Anopheles gambiae str. PEST])

HSP 1 Score: 560.836 bits (1444), Expect = 0.000e+0
Identity = 274/438 (62.56%), Postives = 323/438 (73.74%), Query Frame = 0
Query:  543 TEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDS-----------------AVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWA------------------------------IVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLT-NAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
             + LPK++ DLV KI+ LR  L   QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKF+ EEGLDYGGVAREWLYLLSHEMLNP YGLFQYS +D Y+LQINPDS                 A+NP+HLSYFHFVGRI+G+AVFH H +DG FT PFYK +LNKPI L DIE VDPDLH+SL WIL N+I+GIID+TFSVE+ +FG+ KV+ELK NG  I V E NK EYVKLYV +R+  GIEQQFL+L KGF ELI  +LL+PFDE+ELEL+I GI  ID+ DWK +TRLKQC A+TP + WFW                               IV+SYS EMRA+LLQFVTGS RVPLQGF+ LQGSTG  GPR FT+HLT + P+ +LPKA TCFNRLDLP Y S+++M +KLTQAVEETCGFAVE
Sbjct:  460 ADLLPKYRSDLVGKIRALRQELQLLQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFKGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDHYSLQINPDSYLKQRKTIHFFPVLFLAAINPEHLSYFHFVGRILGIAVFHNHVLDGGFTLPFYKQLLNKPITLSDIEDVDPDLHRSLTWILENNITGIIDSTFSVENNSFGVLKVHELKPNGASIAVTEDNKREYVKLYVNYRFMRGIEQQFLALSKGFGELILSHLLRPFDERELELLISGISKIDVNDWKANTRLKQCTADTPQIVWFWQVSSTDLQLSMSEDRLHVFNWLPFASCFFWQIVESYSPEMRAQLLQFVTGSCRVPLQGFRALQGSTGAVGPRLFTIHLTADVPLQNLPKAHTCFNRLDLPMYDSYQLMYDKLTQAVEETCGFAVE 897          

HSP 2 Score: 231.876 bits (590), Expect = 6.605e-63
Identity = 142/389 (36.50%), Postives = 204/389 (52.44%), Query Frame = 0
Query:   26 LPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG-LPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSND-----------LPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGT-----LSITNSSKNNNLSKTTTTTTXXSTPTS--------EGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQIT-------------------------QPDNLPPGYI-MKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNE--ELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            LPDPFA++ V+ T   + +E  K + DP+WN+HYDL L  N+S  ++++N +KM K+     FLGCVR+  ++IQ ++DTGYQRLDLV  ++    PVKGQI++SLLSRD    G+  A+V   G++    D           LP GWEER   +GR YYVNH  +TTQW+RPTEPA     + G+         L++  +     +           +PTS         G  ++P   S + T +  +++ + N+  S    ++   S   + + I                          + D L  G   ++TT+ GQ+Y+ H  T Q+TWHDPR+ R   +       LGPLP GWEQR T S R YFV+HNN TTQF+DPRI
Sbjct:   23 LPDPFAKILVEGTTQEYTTEICKASLDPRWNSHYDLFLGKNDSILITIYNHRKMPKR---QAFLGCVRIAASSIQLLRDTGYQRLDLVKRSQDDPDPVKGQIIVSLLSRDSATGGTPLAIVSPAGDVRVPEDDDAADESMIHQLPRGWEERSAQNGRTYYVNHYTKTTQWSRPTEPAGPPVRQSGNNNAANSSTPLTVNGTVNGGGVPHPQQQQHILGSPTSATNGVGEESGCPTIPAGPSKSATNHSINSIQS-NDSGSRRHSAEILVSSNNKENCIQQHPSSQQQHHNGKEQRTGKDVENGGRGDALVNGNAEIRTTQQGQVYFYHIPTKQSTWHDPRIPRDFDTQNLTTETLGPLPHGWEQRKTASGRVYFVDHNNRTTQFTDPRI 407          
BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Match: gb|KPM09387.1| (E3 ubiquitin-protein ligase SMURF2-like protein [Sarcoptes scabiei])

HSP 1 Score: 498.049 bits (1281), Expect = 1.571e-162
Identity = 222/317 (70.03%), Postives = 270/317 (85.17%), Query Frame = 0
Query:  550 KKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQG 866
            +++LV K+  LR  L   QP +G+C +EVSR+++FEDSYR I+K+R K +RK+L +KF++EEGLDYGG+AREWLYLLSHEMLNPYYGLFQY+ ED+YTLQINPDS++NPDHLSYFHFVGR+IG+AVFHGH+IDG FT PFYKM+LNKPI LDDIE+VDP+L++SL+WIL NDI+ ++D TFSV H++FG  +V ELKENGKD+ V E NK EYV+LYV FR+  GIEQQF +LQKGF ELIPQ+LL  FDEKELELVIGG+G ID+ DWK HTRLK C +++ +VQWFW  V SYS E RARLLQFVTGSSRVPLQG
Sbjct:  473 RRNLVQKMSTLRQNLQTFQPPSGHCRIEVSREDIFEDSYRCILKLRPKDLRKRLMIKFKDEEGLDYGGIAREWLYLLSHEMLNPYYGLFQYTREDIYTLQINPDSSINPDHLSYFHFVGRVIGIAVFHGHYIDGGFTLPFYKMLLNKPITLDDIESVDPELYRSLRWILDNDITNVVDMTFSVNHDSFGEIQVKELKENGKDLAVTEQNKKEYVQLYVNFRFTNGIEQQFRALQKGFCELIPQHLLVGFDEKELELVIGGLGKIDLNDWKQHTRLKHCTSDSKIVQWFWKAVHSYSEERRARLLQFVTGSSRVPLQG 789          

HSP 2 Score: 164.466 bits (415), Expect = 8.193e-41
Identity = 124/377 (32.89%), Postives = 178/377 (47.21%), Query Frame = 0
Query:   69 FTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDL----VSDTKIGLPVKGQIVISLLSRDGHGTGS---------LNAVVDTLGNLTCS---------------------------------NDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTT--------------------------TTTTXXSTPTSEGANSLPIAS--------SHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNL----GPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
              +S+WN KK+HKK  + GFLGCV++M N +Q +KD GYQRLDL      D  I   +KGQIVIS++S+D     S         + ++ + L  LT S                                 +DLPEGW E R+ + RVYY+NHV RTTQW RP+ PA+   +   ++  + SITN+   +  ++++                          T  +  ST +    NS P  S         H        N  A  NQ   + ++   +      H  ++P  +P G+ M+TT  GQ+Y+    +GQ+TW DPR+   L  N ++NL    GPL  GWE R T+  R YFV+H N TTQF+DPR+
Sbjct:    1 MIISIWNQKKIHKK--HGGFLGCVKIMPNLLQSLKDAGYQRLDLHRYRADDADI---IKGQIVISVISKDSIPKNSVVKNSDDVVIASIPNDLSALTGSGNNRSNEINNNGNDCNGSSDAVLLAARSYVEGVDDLPEGWTECRSSNDRVYYINHVTRTTQWERPSLPAAL--SNNFNQISSNSITNTPNRSTSARSSRHTIQRQHQTLLAQAHNRSYHNINHQTQGSIDSTNSVSSCNSAPAISVSQHSSRNQHQRRSTRHRNYLA-RNQLHEAVITALANVNNAENHPASEPP-MPNGFEMRTTSQGQVYFHQTESGQSTWIDPRVPSELL-NMDINLDELVGPLGCGWEVRHTNGGRRYFVDHTNRTTQFTDPRL 367          
BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Match: XP_006571319.1 (PREDICTED: E3 ubiquitin-protein ligase NEDD4 isoform X5 [Apis mellifera])

HSP 1 Score: 387.882 bits (995), Expect = 1.478e-120
Identity = 246/626 (39.30%), Postives = 349/626 (55.75%), Query Frame = 0
Query:  327 LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDN---SPTNPSRGTSKNEXATNVLPTNPSLSPETNDT-HSNVRNSEPADSPS----TSNE----------APLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPN-TPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKM-RYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLK-QCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFA 930
            + PLPPGWE+R   + R Y+VNH    TQ+  P     + + N   + N   + T   R    +    N L ++ + S ++ D+   + R+SE  +SP      ++E          AP         +  TTT   P +  PS+I N    ++  +T+      PR + + QL P   LP      + +          T ++    P  + P I GP                      + +D   K + L+S L           ++V R  + EDSYR I  + R ++++ KL V+F  E GLDYGG+AREW +LLS EM NPYYGLF+YS+ D YTLQINP S V N +HL+YF F+GRI GMAV+HG  +D  F  PFYKMML K I+L D+E+VD + + SL WI  ND S + + TF V+ E+FG T   ELK +G +IP+ + NK EY+ L +Q+R+   +++Q  +  +GFN LIP  L+K FDE ELEL++ GI  ID+ DWK +T  K    A   VVQWFW +V S+S+EMR+RLLQFVTG+SRVP+ GFK+L GS    GP+ FT+     P ++ P+A TCFNR+DLP Y S++ + EKL +A+E + GFA
Sbjct:  200 IRPLPPGWEERQDANGRTYYVNHIARFTQWERPTESTTSPSANMTIERNFNTAATEFQRRFHISADDENRLRSSINQSDDSVDSPAGSRRSSEQIESPKPVIPANDEGLPPGWGMQIAPNGRVFFIDHTARTTTWIDPRTGRPSSIPN----HIAPSTT------PRSD-LDQLGP---LPEGWEERVHTDGRIFFIDHNTRTTQWEDPRMSNPQIAGPAV-------------------PYSRDYKRKYEYLKSQLRKPNNVPNKFEIKVGRNNILEDSYRIISSVNRVEILKTKLWVEFEGEVGLDYGGLAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPFSGVCNEEHLNYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLGKSIDLKDMESVDSEYYNSLLWIKENDPSEL-ELTFCVDEESFGHTSQRELKPDGANIPLTDENKDEYIALVIQWRFVSRVQEQMNAFLEGFNALIPPTLVKIFDEHELELLMCGIQHIDVRDWKQNTLYKGDYHANHIVVQWFWRVVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGS---NGPQLFTIEKWGTP-ENYPRAHTCFNRIDLPPYESYQQLREKLIKAIEGSQGFA 787          

HSP 2 Score: 131.339 bits (329), Expect = 2.375e-30
Identity = 112/410 (27.32%), Postives = 172/410 (41.95%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDV-----DATGVTHVSEAAKNTTDPKWNTHYDLHLSP-NESFTLSVWNDKKMHKKTYNSGFLGCVRLM---------GNAI-----------------QRIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSRDGHGTGSL----NAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQP--------DNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTR----------ALTSNEELN-LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            K+RL ++    LAK+D+F   DP+ RVD+     D T  + +++  K T +P W   +   + P +    L V+++ ++ +      FLG V L          G  I                  R+K T       +SD+       G       + D  G   +    N+ V+   ++     LP GWEER+  +GR YYVNH+ R TQW RPTE                S T+ S N  + +   T                      ++ +  + L +  NQ S  S+  P  S +    QI  P        + LPPG+ M+   +G++++I      TTW DPR  R          + T   +L+ LGPLP GWE+RV    R +F++HN  TTQ+ DPR+
Sbjct:   26 KLRLKVIAGHQLAKKDIFGASDPYVRVDLNTISGDQTIDSALTKTKKKTLNPVWEEEFIFRVKPVDHKLVLQVFDENRLTR----DDFLGMVELTLLNLPKEQEGRTIPVRRYVLRPRSNHSSQRSRVKGTLEVYHAYISDSSNADNDNGDT-----TSDSGGWEMVQPVNNSPVEQAADVILIRPLPPGWEERQDANGRTYYVNHIARFTQWERPTE----------------STTSPSANMTIERNFNTAATE------------FQRRFHISADDENRLRSSINQ-SDDSVDSPAGSRRS-SEQIESPKPVIPANDEGLPPGWGMQIAPNGRVFFIDHTARTTTWIDPRTGRPSSIPNHIAPSTTPRSDLDQLGPLPEGWEERVHTDGRIFFIDHNTRTTQWEDPRM 396          
BLAST of EMLSAG00000006171 vs. nr
Match: gi|954547610|ref|XP_014616351.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF2 isoform X2 [Polistes canadensis])

HSP 1 Score: 919.072 bits (2374), Expect = 0.000e+0
Identity = 505/993 (50.86%), Postives = 619/993 (62.34%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVS----DTKIGLPVKGQIVISLLSRDGHG-----TGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARP-----------------------TEPASCGT--------------TRRGDRGGTLSIT---NSSKNNNLSKTTTTTTXXST----------PTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQP-----RRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNE-ELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTP-SSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLP-AYPSFEIMAEKLTQAVEETCGFAVE 932
            KIRLTILCARNLA++DLFRLPDPFA++ VD +G  H ++  K T  PKWN HYDL++  N+  T+SVWN +K+HKK    GFLGCVR++ N IQR+KDTGYQRLDL      DT +   VKGQIV+SLLSRDGH      T   NAVVD LG+L+C  DLP+GWEERRT SGR+YY+NH  RTTQW RP                       T P+  G+              T   +  GT S T   +SS  N    T T     S           P+S   +  P+ SS    GN    +   NN           +         RR++  QP +LP GY M+ T+ GQ+Y+ H  TG +TWHDPR+ R L +NE    LGPLP GWE R T S R YFV+HNN TTQF+DPR+                         ++  +N+L          N++ +N  +  P +SP+ +NE P++V              TP SS+ P   TN     +   ++NN+               P L  S+           PP QT S                   P++ +        +E LPK+K+DLVAK+K LR+ LN  QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKFR EEGLDYGGVAREWLYLLSHEMLNP YGLFQYS +D YTLQINPDS +NP+HLSYFHF GRIIG+AVFHGH IDG FTTPFYKM+LNK I L DIE VDP+LH+SL W+L N I G++D TFSVEH +FG+ K +ELK  GKDI V E NK EYV+LYV +R+  GIEQQFL+LQKGF+ELIP +LL+PFDE+ELELVIGG+GTIDI DWK HTRLK C  +TPVV+WFW IV+SY  EMRARLLQFVTGSSRVPLQGFK LQGSTG +GPR FT+H  +AP ++LPKA TCFNR+D+P +YP ++ M +KLTQAVEETCGFAVE
Sbjct:   14 KIRLTILCARNLARKDLFRLPDPFAKITVDGSGQCHSTDTCKATLAPKWNQHYDLYIGKNDGITISVWNHRKIHKKQ-GGGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSEDTDV---VKGQIVVSLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTQSGRLYYINHYTRTTQWIRPNNRPSNAIIPTTPEPPPLPNSEPTSPSGSGSPPNVRTDSPTVLQSTPEWNGEGTSSRTPSRDSSAQNTPRNTPTQQQNRSQQSQQHMRNTPPSSAIRDRRPVRSSDEQNGNQNSRIERSNNGQPRRPNRSNRNRNNAVLSAERRNESLQPMDLPRGYEMRKTQQGQVYFYHVPTGTSTWHDPRIPRDLPANELTTELGPLPSGWEMRQTQSGRVYFVDHNNRTTQFTDPRL------------------------SSQIISNLLK-------RQNNSTNNQVSGNPNNSPAATNE-PVTV-------------DTPVSSIPPQISTNQQRPRIENGSNNNS---------------PTLETST-----------PPTQTVSD-----------------LPKELME-------SELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLSDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIQVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPSLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGASGPRLFTIHAVDAPSENLPKAHTCFNRIDIPQSYPCYQKMLDKLTQAVEETCGFAVE 907          
BLAST of EMLSAG00000006171 vs. nr
Match: gi|954547606|ref|XP_014616349.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF1 isoform X1 [Polistes canadensis] >gi|954547608|ref|XP_014616350.1| PREDICTED: E3 ubiquitin-protein ligase SMURF1 isoform X1 [Polistes canadensis])

HSP 1 Score: 918.687 bits (2373), Expect = 0.000e+0
Identity = 505/993 (50.86%), Postives = 620/993 (62.44%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVS----DTKIGLPVKGQIVISLLSRDGHG-----TGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARP-----------------------TEPASCGT--------------TRRGDRGGTLSIT---NSSKNNNLSKTTTTTTXXST----------PTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQP-----RRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNE-ELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTP-SSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLP-AYPSFEIMAEKLTQAVEETCGFAVE 932
            KIRLTILCARNLA++DLFRLPDPFA++ VD +G  H ++  K T  PKWN HYDL++  N+  T+SVWN +K+HKK    GFLGCVR++ N IQR+KDTGYQRLDL      DT +   VKGQIV+SLLSRDGH      T   NAVVD LG+L+C  DLP+GWEERRT SGR+YY+NH  RTTQW RP                       T P+  G+              T   +  GT S T   +SS  N    T T     S           P+S   +  P+ SS    GN    +   NN           +         RR++  QP +LP GY M+ T+ GQ+Y+ H  TG +TWHDPR+ R L +NE    LGPLP GWE R T S R YFV+HNN TTQF+DPR+                         ++  +N+L      +   N++ +N  +  P +SP+ +NE P++V              TP SS+ P   TN     +   ++NN+               P L  S+           PP QT S                   P++ +        +E LPK+K+DLVAK+K LR+ LN  QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKFR EEGLDYGGVAREWLYLLSHEMLNP YGLFQYS +D YTLQINPDS +NP+HLSYFHF GRIIG+AVFHGH IDG FTTPFYKM+LNK I L DIE VDP+LH+SL W+L N I G++D TFSVEH +FG+ K +ELK  GKDI V E NK EYV+LYV +R+  GIEQQFL+LQKGF+ELIP +LL+PFDE+ELELVIGG+GTIDI DWK HTRLK C  +TPVV+WFW IV+SY  EMRARLLQFVTGSSRVPLQGFK LQGSTG +GPR FT+H  +AP ++LPKA TCFNR+D+P +YP ++ M +KLTQAVEETCGFAVE
Sbjct:   14 KIRLTILCARNLARKDLFRLPDPFAKITVDGSGQCHSTDTCKATLAPKWNQHYDLYIGKNDGITISVWNHRKIHKKQ-GGGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSEDTDV---VKGQIVVSLLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTQSGRLYYINHYTRTTQWIRPNNRPSNAIIPTTPEPPPLPNSEPTSPSGSGSPPNVRTDSPTVLQSTPEWNGEGTSSRTPSRDSSAQNTPRNTPTQQQNRSQQSQQHMRNTPPSSAIRDRRPVRSSDEQNGNQNSRIERSNNGQPRRPNRSNRNRNNAVLSAERRNESLQPMDLPRGYEMRKTQQGQVYFYHVPTGTSTWHDPRIPRDLPANELTTELGPLPSGWEMRQTQSGRVYFVDHNNRTTQFTDPRL------------------------SSQIISNLL------NRRQNNSTNNQVSGNPNNSPAATNE-PVTV-------------DTPVSSIPPQISTNQQRPRIENGSNNNS---------------PTLETST-----------PPTQTVSD-----------------LPKELME-------SELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLSDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIQVTEENKKEYVRLYVNYRFMRGIEQQFLALQKGFHELIPPSLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGASGPRLFTIHAVDAPSENLPKAHTCFNRIDIPQSYPCYQKMLDKLTQAVEETCGFAVE 908          
BLAST of EMLSAG00000006171 vs. nr
Match: gi|972174080|ref|XP_015179970.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF1 [Polistes dominula] >gi|972174082|ref|XP_015179977.1| PREDICTED: E3 ubiquitin-protein ligase SMURF1 [Polistes dominula])

HSP 1 Score: 914.45 bits (2362), Expect = 0.000e+0
Identity = 500/992 (50.40%), Postives = 615/992 (62.00%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVS----DTKIGLPVKGQIVISLLSRDGHG-----TGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARP-----------------------TEPASCGT--------------TRRGDRGGTLSIT---NSSKNNNLSKTTTTTTXXST----------PTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQP-----RRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNE-ELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLP-AYPSFEIMAEKLTQAVEETCGFAVE 932
            KIRLTILCARNLA++DLFRLPDPFA++ VD +G  H ++  K T  PKWN HYDL++  N+  T+SVWN +K+HKK    GFLGCVR++ N IQR+KDTGYQRLDL      DT +   VKGQIV+SLLSRDGH      T   NAVVD LG+L+C  DLP+GWEERRT  GR+YY+NH  RTTQW RP                       T P+  G+              T   +  GT S T   +SS  N    T T     S           P+S   +  P+ S+    GN    +   NN           +         RR++  QP +LP GY M+ T+ GQ+Y+ H  TG +TWHDPR+ R L +NE    LGPLP GWE R T S R YFV+HNN TTQF+DPR+                         ++  +N+L      +   N++ +N  +  P +SP+ +NE             S    +  SS+ P   TN     +   ++NN+               P L  S+           PP QT S                   P++ +        +E LPK+K+DLVAK+K LR+ LN  QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKFR EEGLDYGGVAREWLYLLSHEMLNP YGLFQYS +D YTLQINPDS +NP+HLSYFHF GRIIG+AVFHGH IDG FTTPFYKM+LNK I L DIE VDP+LH+SL W+L N I G++D TFSVEH +FG+ K +ELK  GKDI V E NK EYV+LYV +R+  GIEQQFL+LQKGF+ELIP +LL+PFDE+ELELVIGG+GTIDI DWK HTRLK C  +TPVV+WFW IV+SY  EMRARLLQFVTGSSRVPLQGFK LQGSTG +GPR FT+H  +AP ++LPKA TCFNR+D+P +YP ++ M +KLTQAVEETCGFAVE
Sbjct:   14 KIRLTILCARNLARKDLFRLPDPFAKITVDGSGQCHSTDTCKATLAPKWNQHYDLYIGKNDGITISVWNHRKIHKKQ-GGGFLGCVRILSNTIQRLKDTGYQRLDLCKAHSEDTDV---VKGQIVVSLLSRDGHNGAVSNTVVHNAVVDVLGDLSCPTDLPDGWEERRTQRGRLYYINHYTRTTQWIRPNNRPSNAIIPTTPEPPPLPNSEPTSPSGSGSPPNVRTDSPTVLQSTPEWNGEGTSSRTPSRDSSAQNTPRNTPTQQQNRSQQSQQHIRNTPPSSAIRDRRPVRSNDEQDGNQNSRIERSNNGQPRRPNRSNRNRNNAVLSAERRNESLQPTDLPRGYEMRKTQQGQVYFYHVPTGTSTWHDPRIPRDLPANELTTELGPLPSGWEMRQTQSGRVYFVDHNNRTTQFTDPRL------------------------SSQIISNLL------NRRQNNSTNNQVSGNPNNSPAATNE-------------SVAVETPVSSIPPQISTNQQRPRIENGSNNNS---------------PTLETST-----------PPTQTVSD-----------------LPKELME-------SELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLSDIEGVDPELHRSLTWMLENSIDGVLDATFSVEHSSFGVLKNHELKPGGKDIQVTEENKREYVRLYVNYRFMRGIEQQFLALQKGFHELIPPSLLRPFDERELELVIGGLGTIDINDWKLHTRLKHCTPDTPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGASGPRLFTIHAVDAPSENLPKAHTCFNRIDIPQSYPCYQKMLDKLTQAVEETCGFAVE 908          
BLAST of EMLSAG00000006171 vs. nr
Match: gi|242015442|ref|XP_002428362.1| (E3 ubiquitin-protein ligase Smurf1, putative [Pediculus humanus corporis] >gi|212512974|gb|EEB15624.1| E3 ubiquitin-protein ligase Smurf1, putative [Pediculus humanus corporis])

HSP 1 Score: 781.556 bits (2017), Expect = 0.000e+0
Identity = 437/940 (46.49%), Postives = 578/940 (61.49%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLV-SDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSP-----ETNDTHS--------NVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            K+RLTILCARNLA++ LFRLPDPFA++ VD +G  H +E  + T DPKWN HYDL++   +  T+SVWN KK+HKK +  GFLGCVR+  +A+QR++DTGYQ L+L  +      PVKGQIV+SLLSRDG  +G+  AVVD+ G+++   DLP GWEER+T +GR+YYVNH  +TTQW RP           GD G T+ + ++  ++N   + +++       ++  NS  +A+  +L   G   L   ++  + S+ ++ FS     R + T+  N   G    T+   QI      +       P+ +             LP G+E R T   + YF +  + T+ + DPRIP                             ++ P+   L P     E   THS        N R ++  D   TS+     +   +    ST +     S    N  N   S  +   +N                   LP S+S            P+ ++S    V  +   I   +   +DP+   L G   E LPK+K+DLV+K+++L++ L+  QPQ+G+C LEVSR+E+FE+SYR ++KMR K +RK+L VKFR EEGLDYGGVAREWLYL+S EMLNP YGLFQYS ED YTLQIN DS VNP+HLSYFHF GR++G A+FHGH ID  FTTPFYKM+L+K I LDDI  VDPDLH+SL+W+L ND++ + D TF+VEHEAFG  +V+ELK  G+DIPV + NK EYVKLYV +R+  GIEQQFL+L KGF E++P NLL+ FDE+ELELV+GGI  ID+ DWK +TRLK C   TPVV WFW IV+ YS EM+ARLLQFVTGSSRVPLQGF+ LQG+TG  GPR FT+HL +AP+ +LPKA TCFNR+++P YP  E M EKLTQAVEETCGFAVE
Sbjct:   14 KVRLTILCARNLARKGLFRLPDPFAKISVDGSGQCHSTEVCRATLDPKWNQHYDLYIGKCDGITISVWNHKKIHKK-HGGGFLGCVRIAASAVQRLRDTGYQCLELCKAKADDPEPVKGQIVVSLLSRDG-VSGNRTAVVDSSGHISNPEDLPPGWEERKTLAGRLYYVNHNTKTTQWIRPNG--------EGD-GDTMQLPDNVNSSNDENSCSSSAQFFRSQNQSLNS-SLANCSSLPC-GESGLITSSDLKNSSNNTEVFSC----RRKHTRIKNAETG----TSNELQI----NSSKNNKQQKPQKSV-----------DLPHGFEMRTTQQGQVYFYHVPSGTSTWHDPRIP----------------------------RDLFPSEAELGPLPPGWEARKTHSGRTYFVDHNSRTTQFNDPRLTSSLINKFLKNRNQNVPSTASGEESVSFESRNNGNCITSEAQNLKNN-------------------LPESNSF-----------PEASTSGIERVAFSDSGIADNKVKTEDPV---LDG---EILPKYKRDLVSKLRMLKAELHALQPQSGHCRLEVSRKEIFEESYRLLMKMRPKDLRKRLIVKFRGEEGLDYGGVAREWLYLVSREMLNPQYGLFQYSREDNYTLQINADSGVNPEHLSYFHFAGRVLGTAMFHGHHIDAGFTTPFYKMLLSKSITLDDIAGVDPDLHRSLQWLLHNDVTQL-DTTFAVEHEAFGELRVHELKVGGRDIPVTQENKKEYVKLYVNYRFKRGIEQQFLALLKGFTEVVPANLLRLFDERELELVLGGISKIDVEDWKQNTRLKHCTLTTPVVVWFWEIVEKYSDEMQARLLQFVTGSSRVPLQGFRALQGTTGAAGPRLFTIHLIDAPIHNLPKAHTCFNRIEIPPYPYKEYMYEKLTQAVEETCGFAVE 852          
BLAST of EMLSAG00000006171 vs. nr
Match: gi|1000769969|ref|XP_015608168.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF2 [Cephus cinctus])

HSP 1 Score: 701.434 bits (1809), Expect = 0.000e+0
Identity = 364/660 (55.15%), Postives = 437/660 (66.21%), Query Frame = 0
Query:  275 RHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELN-LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLP-AYPSFEIMAEKLTQAVEETCGFAVE 932
            R    QP +LP GY M+ T+ GQ+Y+ H  TG +TWHDPR+ R L +NE  N LGPLP GWE R T S R YFV+HNN TTQF+DPR+     +N      N  TN        + A N+  T P                       T  EAP    C+ S +  TT S+      P+   N + + V   ++NNT                                     TT+ S+ S  N   V   P+   +           +E LPK+K+DLVAK+K LR+ LN  QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKFR EEGLDYGGVAREWLYLLSHEMLNP YGLFQYS +D YTLQINPDS +NP+HLSYFHF GRIIG+AVFHGH IDG FTTPFYKM+LNK I L DIE VDP+LH+SL W+L N I G++D  FSVEH +FG+ + +ELK  GK+I V E NK EYV+LYV +R+  GIEQQFL+LQKGF+ELIP  LL+PFDE+ELELVIGG+GTIDI DWK HTRLK C  ETPVV+WFW IV+SY  EMRARLLQFVTGSSRVPLQGFK LQGSTG +GPR FT+H  +AP ++LPKA TCFNR+D+P +YPS++ M +KLTQAVEETCGFAVE
Sbjct:  340 RRSENQPSDLPRGYEMRKTQQGQVYFYHVPTGASTWHDPRIPRDLPANELANDLGPLPSGWEMRQTQSGRVYFVDHNNRTTQFTDPRLSSQIISNLLKRQSNGTTN--------QTAGNISSTTP-----------------------TRTEAP----CTESTAAPTTPSN------PTTNPNQSRTRVEGASNNNT-------------------------------------TTAESTPSTQNGQTVSDLPKELME-----------SELLPKYKRDLVAKLKCLRAELNALQPQSGHCRLEVSRNEIFEESYRLIMKMRSKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSRDDNYTLQINPDSGINPEHLSYFHFAGRIIGIAVFHGHHIDGGFTTPFYKMLLNKAITLSDIEGVDPELHRSLTWMLENSIDGVLDAAFSVEHSSFGVLRNHELKPGGKEISVTEENKREYVRLYVNYRFMRGIEQQFLALQKGFHELIPPQLLRPFDERELELVIGGLGTIDINDWKMHTRLKHCTPETPVVKWFWQIVESYGEEMRARLLQFVTGSSRVPLQGFKALQGSTGASGPRLFTIHAVDAPSENLPKAHTCFNRIDIPQSYPSYQKMLDKLTQAVEETCGFAVE 910          

HSP 2 Score: 234.572 bits (597), Expect = 5.285e-61
Identity = 110/186 (59.14%), Postives = 140/186 (75.27%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSD-TKIGLPVKGQIVISLLSRDGH-----GTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARP 186
            KIRLT+LCARNLA++DLFRLPDPFA++ V+ +G  H ++  K T DPKWN ++DL++  N+  T++VWN +K+HKK    GFLGCV ++ + IQR+KD+GYQRLDL  D  +    VKGQIV+S+LSRDGH      T   NAVVD LG+L+C  DLP+GWEERRT SGR+YYVNH  RTTQW RP
Sbjct:   14 KIRLTLLCARNLARKDLFRLPDPFAKITVNGSGQCHSTDTCKATLDPKWNQYFDLYIGKNDGITITVWNHRKVHKKQ-GGGFLGCVCILPHTIQRLKDSGYQRLDLCKDYMETTDVVKGQIVVSMLSRDGHNGAVSNTVGHNAVVDVLGDLSCPTDLPDGWEERRTESGRLYYVNHYTRTTQWIRP 198          
BLAST of EMLSAG00000006171 vs. nr
Match: gi|347602183|gb|AEP16400.1| (SMAD specific E3 ubiquitin protein ligase [Mnemiopsis leidyi])

HSP 1 Score: 692.96 bits (1787), Expect = 0.000e+0
Identity = 393/937 (41.94%), Postives = 537/937 (57.31%), Query Frame = 0
Query:    6 TKIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKK---TYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSI----ISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGII-DNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQG-STGQTGPRHFTLHLTNAPV-DSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            T++R TI+CA+ LAKR+ FRLPD FA++ VD +G  + ++  K T DPKWN H+DL +   +  T+ +WN +K+  K   T    FLGC R+ G  I R+KD G QRL L         +KG IVISL SR+     S           +  N+LPEGWEER   +GR+YYVNHV RT+ W RP        TR       L     ++ N + +T               ++L     H L  +         + ++P  L   F                 P  +   T  GQ+YY +  TG  ++HDPR  R +  +EEL  GPLPPGWE R+  + RPYFV+HN  TTQF DPR+                          E    + P N +L               PA   +T+N    + +   + +  +       S+   +  +T  SN+   +           V+  +SP P  P+SS  S+     +S P  P  Q +  +  S  N+  ++       Q PL+          + + K+ LV K+K+LRS L   Q  +GYC +EV+R+EVFEDSYR+++ +R + +RK+L +KFR EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYS ED+YTL++NP+S++NPDH+SYF+FVGRI+GMA+FHGH+ID  FT PFYK +L +   ++D+E VDP  +KS+KWIL ND+S I  D TF+++H++FG    YEL   GK+  V E+NK EYV LYV++R   G EQQ  +LQKGF E++P++LL  FDEKELEL++ G+G +DI DW+ +T+LK C  ++ +V+WFW IV+   +E RARLLQFVTGSSRVP+ GF  L+G ST  +GPR FT+HL N+    SLPKA TCFNRLDLP Y SFE+M  K+  A+EET GF VE
Sbjct:   13 TRVRFTIVCAKGLAKREFFRLPDVFAKIIVDGSGQHYTTKTVKGTLDPKWNDHFDLFVDHLDCITIQLWNHRKLKSKSCKTVQEAFLGCARIKGQYIFRMKDNGCQRLALGKIRHEDPEIKGIIVISLSSRELFHPPS--------PTFSDPNELPEGWEERTNSNGRLYYVNHVTRTSTWERP--------TRSAYEYAQLDRRRVTEPNIIMRT---------------DNLANRRQHYLNRSSL-------HPNTPQELYGQF-----------------PVIVKCVTPRGQVYYHNRLTGAQSFHDPRFPRHMPISEEL--GPLPPGWELRMAPNGRPYFVDHNTKTTQFKDPRV--------------------------EQLAPISPENRTL---------------PATYVTTTNSQSSAGSLGRNSTMGSNGMEGRGSINSIDTCSTLSSNLPPPSPLTPG-----PVVTTISPGPP-PLSSIPSLEQVERNSDPAHPILQPSDENQPSSDNSSSIL-------QRPLNTIPTSEEQMAMIRNKRGLVQKLKILRSELAKLQTPSGYCRIEVTREEVFEDSYREVMSLRVRDLRKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSREDIYTLEVNPNSSINPDHISYFYFVGRIVGMAIFHGHYIDAGFTLPFYKQLLGRKCTVEDMENVDPAFYKSMKWILENDVSSIFEDQTFTIDHDSFGRHCEYELMPGGKEQRVTENNKKEYVDLYVEWRLKNGTEQQTGALQKGFYEVVPKHLLSAFDEKELELIVCGLGHVDIDDWRANTKLKGCSPDSNIVKWFWKIVEEMDNEKRARLLQFVTGSSRVPISGFSGLRGSSTVNSGPRPFTIHLVNSMSGGSLPKAMTCFNRLDLPEYVSFEVMRNKIITAIEETMGFNVE 838          
BLAST of EMLSAG00000006171 vs. nr
Match: gi|1121118511|ref|XP_019548334.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF2-like [Aedes albopictus])

HSP 1 Score: 669.848 bits (1727), Expect = 0.000e+0
Identity = 347/653 (53.14%), Postives = 425/653 (65.08%), Query Frame = 0
Query:  283 NLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEEL--NLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLT-NAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            +LPPGY M+TT+ GQ+Y+ H  T  +TWHDPR+ R   +       LGPLP GWEQR T S R YFV+HNN TTQF+DPR+                         N     ++                 + ++P    STS     +  CSS+     T  S  S   P                             Q+S   +  V+ SS ++     PP    + S+  SV  TP  +  P   P  P  +     G E LPK+++DLV KI+ LR  L   QPQ+G+C LEVSRQE+FE+SYR I+KMR + MRK+L VKFR EEGLDYGGVAREWL+LLS EMLNP YGLFQYS +D Y+LQINPDS +NPDHLSYFHFVGRI+G+AVFH H +DG FT PFYK +LNKPI L+DIE VDP+LH+SL W+L N+I+G+ID+TFSVE+ +FG  KV+ELK NG  + V E NK EYVKLYV +R+  GIEQQFL+L KGF ELIP  LL+PF+E+ELELVIGGI  ID+ DWK HTRLKQC  ETP + WFW IV+SYS EMRA+LLQFVTGS RVPLQGF+ LQGSTG  GPR FT+HLT +AP+ +LPKA TCFNR+DLP Y S+++M +KLTQAVEETCGFAVE
Sbjct:  567 DLPPGYEMRTTQQGQVYFYHIPTKMSTWHDPRIPRDFDTQNIAPETLGPLPHGWEQRKTASGRVYFVDHNNRTTQFTDPRL-------------------------NGHILQII----------------RKQAQPPAGQSTSAGGSATAGCSSTNQVVATAISYLSGAGP-----------------------------QVSNGALGAVAGSSRLMM----PPEATASGSNGLSVEPTPHRV-PPNNIPDLPQGLL---DGAESLPKYRRDLVGKIRSLRVELQALQPQSGHCRLEVSRQEIFEESYRLIMKMRPRDMRKRLMVKFRGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSGDDRYSLQINPDSGINPDHLSYFHFVGRILGIAVFHNHVLDGGFTLPFYKQLLNKPITLNDIEDVDPELHRSLTWMLENNINGVIDSTFSVENNSFGALKVHELKPNGASVQVTEDNKREYVKLYVNYRFMRGIEQQFLALSKGFGELIPSQLLRPFEERELELVIGGISKIDVNDWKTHTRLKQCTPETPQIIWFWQIVESYSPEMRAQLLQFVTGSCRVPLQGFRALQGSTGAVGPRLFTIHLTADAPIQNLPKAHTCFNRIDLPMYDSYQLMYDKLTQAVEETCGFAVE 1141          

HSP 2 Score: 222.631 bits (566), Expect = 2.021e-56
Identity = 107/193 (55.44%), Postives = 140/193 (72.54%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIGL-PVKGQIVISLLSRDGHGTGSLNAVVDTLGNLTCSND--------LPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPA 190
            KIR+TILCARNLA++DLFRLPDPFA++ VD T   + +E  K + +PKWNTHYDL+L  N++ T+S+WN +K+ K+   SGFLGCVR++ + IQR+KDTGYQRL+L   +     PVKGQI++SL+SRDG   G+  A+V   G++   +D        LP GWEER T + RVYYVNHV +TTQW RPTEPA
Sbjct:   14 KIRITILCARNLARKDLFRLPDPFAKIHVDGTAQEYKTEICKASLEPKWNTHYDLYLGKNDNITISIWNQRKVQKRQ-GSGFLGCVRIVSSTIQRLKDTGYQRLELCKASHDDPDPVKGQIIVSLMSRDGTTGGTPLAIVGPAGDVRGPDDDEEILKDELPAGWEERLTQTNRVYYVNHVTKTTQWDRPTEPA 205          
BLAST of EMLSAG00000006171 vs. nr
Match: gi|1058258900|gb|JAT32935.1| (hypothetical protein g.48621, partial [Graphocephala atropunctata])

HSP 1 Score: 633.254 bits (1632), Expect = 0.000e+0
Identity = 338/644 (52.48%), Postives = 425/644 (65.99%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLM-------------NDANNHHHDDNSP-TNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRN-----------SEPADSP-----STSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVS------NVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGT-----EFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T + R ++VNH+  TTQ+  P  P +              D  N   ++NS   NP++  +++E         P + P      SN R+           ++P D P      T+ +  +      +GS++      P     + +T   +        VR T S             Q +  P L  S  S+II    P    Q+ + SS + P++P   GG +          +          E LPK+++DLVAK K+LR+ L   QPQ+G+C LEVSR E+FE+SYR I+KMR K MRK+L VKFR EEGLDYGGVAREWLYLLSHEMLNP YGLFQYS ED YTLQIN DS+VNP+HLSYFHF GRIIG+AVFHGH+IDG FTTPFYKM+LNKPI L+DIE VDP+LH+SL W+L ND++G+ID +F+VE  +FG+ KV+ELK  GKDIPV E NK EYVKLYV +R+  GIEQQFL+LQKGF E++P  L++PFDE+ELEL+IGG+G+IDI DWK +TRLK C AETPVVQWFW IV++YS EMRARLLQFVTGSSRVPLQGFK LQGSTG  GPR FT+H+ +AP ++LPKA TCFNR+DLPAY  ++ + EKLTQAVEETCGFAVE
Sbjct:   28 LPEGWEERRTSAGRLFYVNHHTKTTQWVRPHRPQILLTPTPESPGSVGPDVENIPQNNNSHLANPNQRHNRDE--------TPCIRPIPGQRRSNRRHQARTAQGSHQLTQPPDLPHGYELRTTQQGQVYFYHIPTGSSTWHDPRIPRECT-AELTAEVLGPLPPGWEVRQTASGRLYYVDHNNRTTQFTD-PRLSTSVISNIIKKNNPAST-QSPAVSSGTNPSSPSS-GGEQTSAATTPVPVVPTTPAAPPEGEALPKYRRDLVAKQKILRAELTALQPQSGHCRLEVSRTEIFEESYRLIMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSREDNYTLQINSDSSVNPEHLSYFHFAGRIIGIAVFHGHYIDGGFTTPFYKMLLNKPITLEDIEGVDPELHRSLTWMLENDLTGVIDTSFAVEVNSFGVLKVHELKTGGKDIPVTEENKKEYVKLYVNYRFMRGIEQQFLALQKGFTEIVPAQLMRPFDERELELIIGGLGSIDIQDWKANTRLKHCTAETPVVQWFWQIVETYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAVGPRLFTIHVIDAPQENLPKAHTCFNRIDLPAYDGYQRLLEKLTQAVEETCGFAVE 659          

HSP 2 Score: 171.785 bits (434), Expect = 3.975e-41
Identity = 117/284 (41.20%), Postives = 143/284 (50.35%), Query Frame = 0
Query:  131 LSRDGHG-TGSLNAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTL-SITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRR---------HQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELN---LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPR---------IPLMNDANNHHHDDNSPTNPSRGTSKNEXAT 391
            LSRDG G T  LNAVVDT G L+C  DLPEGWEERRT +GR++YVNH  +TTQW RP  P    T      G     + N  +NNN                          SH    N        N  ++P     P      RR         HQ+TQP +LP GY ++TT+ GQ+Y+ H  TG +TWHDPR+ R  T+  EL    LGPLPPGWE R T S R Y+V+HNN TTQF+DPR         I   N A+      +S TNPS  +S  E  +
Sbjct:    1 LSRDGGGGTAGLNAVVDTHGGLSCPADLPEGWEERRTSAGRLFYVNHHTKTTQWVRPHRPQILLTPTPESPGSVGPDVENIPQNNN--------------------------SHLANPNQRH-----NRDETPCIRPIPGQRRSNRRHQARTAQGSHQLTQPPDLPHGYELRTTQQGQVYFYHIPTGSSTWHDPRIPRECTA--ELTAEVLGPLPPGWEVRQTASGRLYYVDHNNRTTQFTDPRLSTSVISNIIKKNNPASTQSPAVSSGTNPSSPSSGGEQTS 251          
BLAST of EMLSAG00000006171 vs. nr
Match: gi|1058005834|gb|JAS06601.1| (hypothetical protein g.17677 [Clastoptera arizonana])

HSP 1 Score: 631.713 bits (1628), Expect = 0.000e+0
Identity = 340/636 (53.46%), Postives = 430/636 (67.61%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIP--LMNDANNH------HHDDNSPT--------NPSRGTSKNEXATNVLPTNPSLS----PETNDTHSNVRNSEPADSPS-----TSNEAPLSVTCSSSGSTSTTTSSTPSSL--APSNITNTTVS------NVRATTSNNTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T + R ++VNH+  TTQ+  P  P  L+N  N +      +   N+P         NP++ T +++     +P     S          H  ++  +P+D P+     T+ +  +      SG+++      P  L    S ++N  +        VR T S             Q +  P L  S  S+II    P     TT  S++++  TP     P      P + +L   G E LPK+++DLVAK K+LR+ L   QPQ+G+C LEVSR E+FE+SYR ++KMR K MRK+L VKFR EEGLDYGGVAREWLYLLSHEMLNP YGLFQYS ED YTLQINPDS+VNP+HLSYFHF GRIIG+AVFHGH+IDG FTTPFYKM+LNKPI LDDIE VDP+LH+SL W+L N+++G+ D TFSVE  +FG+ +V+ELK  GKDIPV E NK EYVKLYV +R+  GIEQQFL+LQKGF EL+P  LL+PFDE+E+EL+IGG+G+IDI DWK +TRLK C A+TPVV+WFW IV+ YS EMRARLLQFVTGSSRVPLQGFK LQGSTG  GPR FT+H+ +AP ++LPKA TCFNR+DLPAY +++ + EKLTQAVEETCGFAVE
Sbjct:  171 LPEGWEERRTSAGRLFYVNHHTKTTQWVRPNRPQILLNSPNTNGCSSPENESTNTPVQTNGNHLANPAQRTREDQPIVRHIPGQRRSSRFRGASVGGRHHQIQ--QPSDLPNGYELRTTQQGQVYFYHIPSGASTWHDPRIPRDLPTQASELSNEALGPLPHGWEVRQTASGRLYYVDHNNRTTQFTD-PRLSGSIISNIIKKHCPNSTGSTTPPSTTNIVTTPTTTVSPSTSTTSPPAPSLEADG-EVLPKYRRDLVAKQKILRAELTALQPQSGHCRLEVSRSEIFEESYRLVMKMRPKDMRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSREDNYTLQINPDSSVNPEHLSYFHFAGRIIGIAVFHGHYIDGGFTTPFYKMLLNKPITLDDIEGVDPELHRSLTWMLENNLTGVFDTTFSVEVNSFGVLRVHELKTGGKDIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFTELVPAQLLRPFDEREMELIIGGLGSIDINDWKQNTRLKHCTADTPVVKWFWQIVELYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAVGPRLFTIHVIDAPQENLPKAHTCFNRIDLPAYENYQRLQEKLTQAVEETCGFAVE 802          

HSP 2 Score: 328.176 bits (840), Expect = 2.265e-95
Identity = 184/367 (50.14%), Postives = 228/367 (62.13%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG-LPVKGQIVISLLSRDGHGTGSL-------NAVVDTLGNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRAL-TSNEELN---LGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            KIRLTILCARNLA++DLFRLPDPFA++ VD +G  H ++  K T DPKWN HYDL++   +  T+SVWN KK+HKK    GFLGCVR+M N I+R+KDTGYQRLDL   +     PVKGQIV+SLLSRDG G G         NAVVD LG L+C  DLPEGWEERRT +GR++YVNH  +TTQW RP  P                + NS   N  S     +T  +TP     N L  A+    T      +  +  Q   S      +S+  R HQI QP +LP GY ++TT+ GQ+Y+ H  +G +TWHDPR+ R L T   EL+   LGPLP GWE R T S R Y+V+HNN TTQF+DPR+
Sbjct:   14 KIRLTILCARNLARKDLFRLPDPFAKISVDGSGQCHSTDTCKATLDPKWNQHYDLYIGKGDGITISVWNHKKIHKKQ-GGGFLGCVRIMPNIIERLKDTGYQRLDLCKASNDDPDPVKGQIVVSLLSRDGGGHGGSGGGTRGHNAVVDPLGGLSCPEDLPEGWEERRTSAGRLFYVNHHTKTTQWVRPNRPQ--------------ILLNSPNTNGCSSPENEST--NTPVQTNGNHL--ANPAQRTREDQPIVRHIPGQRRSSRFRG--ASVGGRHHQIQQPSDLPNGYELRTTQQGQVYFYHIPSGASTWHDPRIPRDLPTQASELSNEALGPLPHGWEVRQTASGRLYYVDHNNRTTQFTDPRL 359          
BLAST of EMLSAG00000006171 vs. nr
Match: gi|939237793|ref|XP_014261551.1| (PREDICTED: E3 ubiquitin-protein ligase SMURF2 [Cimex lectularius] >gi|939237795|ref|XP_014261552.1| PREDICTED: E3 ubiquitin-protein ligase SMURF2 [Cimex lectularius])

HSP 1 Score: 629.787 bits (1623), Expect = 0.000e+0
Identity = 334/632 (52.85%), Postives = 417/632 (65.98%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDP---RIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNP-----SLSPETNDTHSNVRN-SEPADSPS-----TSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVS-----NVRATTSNNTVLRPRGEVIQQ-----LSPF-----PVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            LP GWE+R T   R ++VNH   TTQ++ P   R    N  N +  ++   +  +R  S N  + + LP  P       S +T  +  +  N  +P+D PS     T+ +  +     ++G +S      P  L    +     S       R T S         +   Q     LS F       L   +++++ +  P P   Q  +++  +V         P   P               LPK+++DLVAK K+LR  L   QPQ+G+C LEVSRQE+FE+SYR ++KMR K +RK+L VKFR EEGLDYGGVAREWLYLLSHEMLNP YGLFQYS+E  YTLQINPDS VNP+HLSYFHFVGRIIG+AVFHGH+IDG FTTPFYKM+LNKPINLDDIE+VD +LH+SL WIL NDI+ +ID TF+VE  +FG+ KV+ELK  GKDI + E NK EYVKLYV +R+  GIEQQFL+LQKGF ELIP +LLK FDE ELEL+IGG+GTIDI DWK +TRLK C + TPVV WFW IV+S+S EMRARLLQFVTGSSRVPLQGFK LQGSTG +GPR FT+H+T+AP ++LPKA TCFNR+DLPAY ++  + +KL QAVEETCGFAVE
Sbjct:  168 LPSGWEERRTPEGRLFYVNHRTETTQWTLPTAERSGGSNSTNTNGRNETPSSTNNRRESLNTSSDSCLPGLPPSQARRRSTKTRSSQGSRHNIQQPSDLPSGYEIRTTEQGQVYFYHVATGVSSWHDPRVPRGLDTEAVGEHLGSLPPGWEARHTPSGRVYYVDHNKRTTQFTHPALSTFLRAGGSGLNGHATNTMTAENPQPNGEQEANANKENVKE-------PEGEP---------------LPKYRRDLVAKQKILRVELGTLQPQSGHCRLEVSRQEIFEESYRLVMKMRAKDLRKRLMVKFRGEEGLDYGGVAREWLYLLSHEMLNPQYGLFQYSTESTYTLQINPDSGVNPEHLSYFHFVGRIIGIAVFHGHYIDGGFTTPFYKMLLNKPINLDDIESVDQELHRSLTWILENDITNVIDTTFAVEFNSFGVLKVHELKTGGKDIQLTEDNKKEYVKLYVNYRFMRGIEQQFLALQKGFTELIPLHLLKTFDENELELIIGGLGTIDIADWKENTRLKHCTSTTPVVVWFWEIVESFSEEMRARLLQFVTGSSRVPLQGFKSLQGSTGASGPRLFTIHVTDAPQENLPKAHTCFNRIDLPAYKTYNKLYDKLCQAVEETCGFAVE 777          

HSP 2 Score: 256.914 bits (655), Expect = 9.983e-70
Identity = 169/424 (39.86%), Postives = 224/424 (52.83%), Query Frame = 0
Query:    7 KIRLTILCARNLAKRDLFRLPDPFARVDVDATGVTHVSEAAKNTTDPKWNTHYDLHLSPNESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQRIKDTGYQRLDLVSDTKIG-LPVKGQIVISLLSRDGHGTGSLNAVVDTLGNLT-----CSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRAL-TSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPT---NPSLSPETNDTHSNVRNSEPADSP 420
            KIRLTIL A  L +R+ FRLPD FA++ VD +G  H ++  K T DP+WN +YDL++S N+  T+SVWN +K+HKK    G+LG V++  NAIQR+KDTGYQ+L+L++        V+G IVISLLS        L        +++      S +LP GWEERRTP GR++YVNH   TTQW  PT   S G             +NS+  N  ++T ++T            SL  +S   L G     LP    +      S    S Q  RH I QP +LP GY ++TTE GQ+Y+ H  TG ++WHDPR+ R L T     +LG LPPGWE R T S R Y+V+HN  TTQF+ P +     A               G+  N  ATN +      P+   E N    NV+  EP   P
Sbjct:   14 KIRLTILGACKL-RREFFRLPDTFAKISVDGSGQCHSTDTCKGTLDPQWNQYYDLYISKNDRITISVWNHRKIHKKD-RGGYLGSVQITSNAIQRLKDTGYQKLELLNLLLPSYYCVRGHIVISLLSWSCGRNSPLREQCSPSSSVSPHTNESSQELPSGWEERRTPEGRLFYVNHRTETTQWTLPTAERSGG-------------SNSTNTNGRNETPSSTNNRR-------ESLNTSSDSCLPG-----LPPSQARRR----STKTRSSQGSRHNIQQPSDLPSGYEIRTTEQGQVYFYHVATGVSSWHDPRVPRGLDTEAVGEHLGSLPPGWEARHTPSGRVYYVDHNKRTTQFTHPALSTFLRAG--------------GSGLNGHATNTMTAENPQPNGEQEANANKENVK--EPEGEP 390          
BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold56_size446035-snap-gene-3.36 (protein:Tk11506 transcript:maker-scaffold56_size446035-snap-gene-3.36-mRNA-1 annotation:"e3 ubiquitin-protein ligase smurf1 isoform x1")

HSP 1 Score: 654.055 bits (1686), Expect = 0.000e+0
Identity = 300/389 (77.12%), Postives = 338/389 (86.89%), Query Frame = 0
Query:  544 EFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            E LPK+K+DLVAKIK+LR  L + QPQ+G+C LEVSRQEVFE+SYRQIVKMR K ++K+L +KFR E+GLDYGG+AREWL+LLSHEMLNPYYGLFQYS +D+YTLQINPDS++NPDHLSYFHFVGRIIGMAVFHGH IDG FTTPFYKM+LNKPINLDDIE VDP+LH SL W+L NDI+GI+D+ FSVEHEAFGL +  ELK+ GKD+ V E NK EYVKLYV +RWC GIEQQFL+LQKGFNELIPQ LLKPFDE+ELELVIGGIG IDI DWK HTRLK C+ +TPVV+WFW IVD YS EMRARLLQFVTGSSRVPLQGFK LQGSTG  GPR FTLH+    +++LPKA TCFNRLDLPAY S   M +KLTQAVEETCGFAVE
Sbjct:  592 EVLPKYKRDLVAKIKLLRQELQSQQPQSGHCRLEVSRQEVFEESYRQIVKMRPKDLKKRLMIKFRGEDGLDYGGIAREWLHLLSHEMLNPYYGLFQYSRDDIYTLQINPDSSINPDHLSYFHFVGRIIGMAVFHGHHIDGGFTTPFYKMLLNKPINLDDIEEVDPNLHTSLHWMLENDITGILDSNFSVEHEAFGLLQSQELKKGGKDLEVTEKNKKEYVKLYVNYRWCRGIEQQFLALQKGFNELIPQQLLKPFDERELELVIGGIGKIDIEDWKTHTRLKHCNTDTPVVKWFWEIVDGYSEEMRARLLQFVTGSSRVPLQGFKALQGSTGAAGPRLFTLHVIEVSLENLPKAHTCFNRLDLPAYESQTKMHDKLTQAVEETCGFAVE 980          
BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold186_size273091-processed-gene-0.4 (protein:Tk00902 transcript:snap_masked-scaffold186_size273091-processed-gene-0.4-mRNA-1 annotation:"e3 ubiquitin-protein ligase nedd-4")

HSP 1 Score: 379.407 bits (973), Expect = 6.641e-113
Identity = 242/620 (39.03%), Postives = 346/620 (55.81%), Query Frame = 0
Query:  330 LPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSR---GTSKNEXATNVLPTNPSLSPET--NDTHS-NVRNSEPADSPSTSNEAPLSVTCSSSGSTSTTTSSTPSSLAPSNITNTTVSNVRATTSN--NTVLRPRGEVIQQLSPFPVLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTP------PVIGGPRCWPQ---------------------DPLSIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLEVSRQEVFEDSYRQIVKM---RYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLK-QCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAY 909
            LP GWE+R   + R Y+VNH   +TQ+  P +   N A     ++N+ +  SR     S +E   ++      L  ++  ++ HS N+   E  D+P   + +  + + +++G      +S PS  APS      +S+V  T  +  NT   P+   +Q      V  ++ S    +   P     ++  S  ++PN P      P+   P  W +                     DP  I+  GG    +P + +D   K   L+S L           ++V RQ + EDS+R +V++   +  V++ KL ++F NE GLDYGG+AREW YLLS EM NPYYGLF+YS+ D YTLQINP S + N DH+SYF F+GRI GMAV+HG  +DG F  PFYKMML K I L D+E+VD + + SL WI  ND + + + TF V+ E FG T   ELK NG  I V   +K +Y+K  +Q+R+   +E+Q    + GFNEL+P +LLK FDE ELEL++ GIG+ID+ DWK +T  K        V+QWFW +V S+++EMR+RLLQFVTG+SRVP+ GFK+L GS    GP+ +T+     P +S P+A TCFNRLDLP Y
Sbjct:  218 LPAGWEERQDANGRTYYVNHVARSTQWQHPAL-TDNTAATAAVNENNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINRGESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPS------LSSVGGTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPL---PEGWEERVHTDARIFFIDHNTRTTQWEDP-RISTIGGPA--IP-YNRDYKRKYDYLKSQLKKPSNVPNKFEIKVRRQFILEDSFRHLVQIPSSKVDVLKTKLWIEFDNEIGLDYGGLAREWFYLLSKEMFNPYYGLFEYSATDNYTLQINPYSGMCNEDHISYFKFIGRIAGMAVYHGKLLDGFFIRPFYKMMLGKTITLKDMESVDTEYYNSLLWIKENDPAEL-ELTFQVDEEQFGTTISRELKPNGAKIAVTNESKNDYIKEVIQWRFVSRVEEQMNEFKAGFNELVPLSLLKIFDEGELELLMCGIGSIDVKDWKTNTVYKGDYHPNHIVIQWFWRVVLSFNNEMRSRLLQFVTGTSRVPMNGFKELYGS---NGPQLYTIEKWGNP-NSFPRAHTCFNRLDLPPY 818          

HSP 2 Score: 328.946 bits (842), Expect = 2.976e-95
Identity = 165/357 (46.22%), Postives = 231/357 (64.71%), Query Frame = 0
Query:  578 VSRQEVFEDSY---RQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAV-NPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLK-QCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGF 929
            V R  +F+DS+   R I +     ++ +L V+F  E GLD+GG+AREW +LLS EM NPYYGLF+YS+ D YTLQINP S + +PDHL+YF F+G++ GMAV+HGH +DG F  PFYKMML++ I L D+E+VD + + SL +I  +D    ++ +F V+ +  G+T   +L   G D+ V E+NK++Y+   + +R+   +++Q  + Q+GF   IP N L  FDE ELEL++ GI  IDI DW  HT  +        VVQWFW  V S+S++MRARLLQFVTG+SRVP+ GF  LQGS        F +    +P DS P++ TCFNRLDLP Y S++ +  +L  AV+    F
Sbjct: 1240 VRRDHIFQDSFERIRTIAQSDPNHLKARLWVEFEGESGLDFGGLAREWFFLLSKEMFNPYYGLFEYSANDDYTLQINPLSGIYHPDHLNYFEFIGQVAGMAVYHGHLLDGFFIRPFYKMMLDRKITLIDMESVDVEYYNSLVYIKDHDPEP-LELSFQVDEDRLGVTVSEDLIPQGADVTVTEANKLDYIDKVIHWRFVSRVQEQMQAFQRGFQRFIPLNDLGVFDEGELELLLCGIDAIDIQDWMKHTVYRGGYHPNHQVVQWFWRSVLSFSNQMRARLLQFVTGTSRVPMNGFVALQGSNKAEA---FCIKGFGSP-DSFPRSHTCFNRLDLPRYTSYKELRSRLMMAVQNAEAF 1591          

HSP 3 Score: 112.464 bits (280), Expect = 3.008e-25
Identity = 96/397 (24.18%), Postives = 170/397 (42.82%), Query Frame = 0
Query:   12 ILCARNLAKRDLFRLPDPFARVDVD------ATGVTHVSEAAKNTTDPKWNTHYDLHLSPN-ESFTLSVWNDKKMHKKTYNSGFLGCVRLMGNAIQ---RIKDTGYQRLDLVSDTKIGLPVKGQIVISLLSR---------------DGHGTGSLNAVVDTLGN-LTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASCGTTRRGDRGGTLSITNSSKNNNLSK------TTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQP-DNLPPGYIMKTT-EHGQIYYIHEGTGQTTWHDPRLTRALT-----------SNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPL 363
            ++   +LAK+D+F   DP+  V +         G+  V+   K + +P W   +    +P+  +    V+++ ++ +      FLG V +    +     + DT Y        +K    V+G++ + + ++               + H TG+  A +  + N +     LPEGWEER++ +GR YYV+H  R      P+          G+ G   + + S       +        T       PT +    + + S H         +P  N +++PS++ +P  S  P    I Q  + LP G+ +K    +G++++I+  T +TTW DPR  R              S E+ +L  LP GWE+++T   R  F++H    TQ+ DPRI L
Sbjct:  820 MISGADLAKKDIFGASDPYVTVSLSQKSNGSTIGLEAVTTVVKKSLNPYWLARFLFRANPDLHNLLFQVFDENRITR----DDFLGLVIVDLKDVATTGHLPDTFYTLRKRSPKSK----VRGRLFLQIENKPDSNPTNTPSSQPQTEPHATGASVAPLPRVANGIIPDAALPEGWEERQSATGRTYYVDHGHREVHCTHPSRACHRIIPADGNTGDVEAQSTSRSTRPFHRERVHISVDTEAEPGGHPTIDDPEEIELGSPHTA-------IPTGNTRETPSNVVEPCQSSAPPERTIPQDTEGLPSGWGLKVAPNNGRVFFINHNTRETTWVDPRTNRPSPLPGRSEDFDDPSQEKDHLMDLPQGWEEKITPDGRYLFIDHMQKQTQWEDPRIAL 1201          

HSP 4 Score: 102.834 bits (255), Expect = 2.726e-22
Identity = 75/250 (30.00%), Postives = 114/250 (45.60%), Query Frame = 0
Query:  149 GNLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPT------------EPASCGTTRR----------------GDRGGTLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSPSSLSDPFSSLQPRRHQITQPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALT---------SNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRI 361
            G+ T    LP GWEER+  +GR YYVNHV R+TQW  P             E  +   +RR                  R G+ S+ +   + N+++        S  T +G ++    +S A TG       +V +  +PS      SS+          + LP  + M+   +G++++I+    +TTW DPR  R  +            E +LGPLP GWE+RV   +R +F++HN  TTQ+ DPRI
Sbjct:  210 GDPTAGLVLPAGWEERQDANGRTYYVNHVARSTQWQHPALTDNTAATAAVNENNASELSRRRVHISMDEQRPSLDIGRSRLGSDSVIDELHSLNINR------GESVDTPDGPSTSGTTTSAAATGGAVPRRTSVPSGSAPS-----LSSVGGTLPSPLNTEGLPQEWTMQVAPNGRVFFINHSEKKTTWVDPRTGRPSSVPSQGNMPNRPHEDDLGPLPEGWEERVHTDARIFFIDHNTRTTQWEDPRI 448          
BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold125_size330479-snap-gene-2.15 (protein:Tk06892 transcript:maker-scaffold125_size330479-snap-gene-2.15-mRNA-1 annotation:"e3 ubiquitin")

HSP 1 Score: 352.829 bits (904), Expect = 2.288e-103
Identity = 268/869 (30.84%), Postives = 406/869 (46.72%), Query Frame = 0
Query:  150 NLTCSNDLPEGWEERRTPSGRVYYVNHVRRTTQWARPTEPASC-------GTTRRGDRGG------------TLSITNSSKNNNLSKTTTTTTXXSTPTSEGANSLPIASSHALTGNGTDNLPAVNNQDSP-SSL----------------SDPFSSLQPRRHQIT-------QPDNLPPGYIMKTTEHGQIYYIHEGTGQTTWHDPRLTRALTSNEELNLGPLPPGWEQRVTDSSRPYFVNHNNHTTQFSDPRIPLMNDANNHHHDDNSPTNPSRGTSKNEXATNVLPTNPSLSPETNDTHSNVRNSEPADSPSTSNEAPLSVTCSSSGSTS--TTTSSTPSSL---------APSNITNTTVSNVRATTSNNTVLRPRGEVIQ------------------QLSPFP------VLPVSSSSSIISSPPPPPPPQTTSSSSSSVPNTPPVIGGPRCWPQDPL-SIALHGGGTEFLPKFKKDLVAKIKVLRSXLNNXQPQNGYCPLE--VSRQEVFEDSYRQIVKMRYKVMRK-KLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISG--IIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQ--CDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            NL  +  LP+ WE R    GR++Y++H+  TT W +P   +S         ++R+ +R              T++ T+ S   ++    T +   S  T+E    L + S        +D L  V   D P +SL                +  +   Q  R  +T       +   LPPG+  K   +G+ ++I      TT+ DPR               LP  W     D S P+ +  N+  +              NH H   S T P   +        +      L+P  +   S     E  +  + S  A   +T S+SG  S  T   STP++           PS +    +   R + ++N  LR +   I+                   LS F       V PV+  +++  SP P  PP    ++S S P +P     P      PL S             +++D  AK++     L +     G   L+  + R+ + ED+++Q++    K + K +L + F  EEGLDYGG +RE+ +L+S E+ NPYYGLF+YS+ D YT+Q++P S+    HL +F F GR++ +A+ + + +D  FT PFYK +L  P++LDD+E++D + H+S+ W+  N+I+    +  TFSV  E FG     +LK  GK + V E NK +Y++  V++R   G+  Q   L KGF E++   LL  FD +ELELVI G   ID+ DW+ +T  +    DA  PV+ WFW  ++ + +E R RLLQFVTG+S VP +GF  L+GS    GPR F +     P  SLP+A TCFNRLDLP Y S E + EKL  AVEET  F +E
Sbjct:  878 NLPSNEALPQHWEARMDSHGRIFYIDHINHTTTWQKPAPSSSVQEALTERASSRQDERQREQLDQRYQRIHRTMASTSVSSEADIVDGITYSESASYITAERQRELLLQSPMVRFICRSDFLTRVQQADHPCASLLNRSSVKHMISRIRRDASTYERYQHNRDLVTLLNVFACKDRELPPGWESKKDRNGKTFFIDHTAKTTTFIDPR---------------LPNDWPLLPLDVSEPHLLAPNDPASPLL-----PPPPPRNHRH---SLTPPPNSSRSAAANAAIF-----LAPPNSRRRSRSVGDEELNQSARSMHAVRPLTSSASGGVSIPTVIFSTPTAYNDKVVAFLRQPSIL--EVLKQKRPSITSNKFLRDKINAIRIEGNLALERLSHDVELTILLSLFEDEIMSFVAPVAILTNL--SPGPSTPPLMIGANSGSGPRSPRNSPNP-----SPLTSPGFARANARAPAPYRRDFDAKLRSFYRKLESKGYGQGPGKLKFPMRREFLLEDAFKQVMCSSKKDLAKSRLFISFDKEEGLDYGGPSREFFFLISREVFNPYYGLFEYSANDTYTVQLSPLSSFVDHHLDWFRFCGRLLALALVNKYLLDAFFTRPFYKALLKLPVSLDDLESLDGEFHQSMVWLQENEITDPEALGMTFSVNEEVFGKVIERDLKPGGKHMQVTEKNKKDYIERVVKWRLERGVAAQTDILVKGFYEVVDPRLLAVFDARELELVIAGTAEIDVSDWRRNTEYRSGYYDAH-PVIAWFWMAIERFDNERRLRLLQFVTGTSSVPYEGFSALRGS---NGPRRFCIEKWGKP-SSLPRAHTCFNRLDLPPYISPEQLLEKLLLAVEETSTFGIE 1704          
BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold450_size166944-processed-gene-0.5 (protein:Tk00124 transcript:snap_masked-scaffold450_size166944-processed-gene-0.5-mRNA-1 annotation:"e3 ubiquitin-protein ligase huwe1-like isoform x5")

HSP 1 Score: 288.115 bits (736), Expect = 1.075e-81
Identity = 138/359 (38.44%), Postives = 217/359 (60.45%), Query Frame = 0
Query:  576 LEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGI-IDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETC---GFA 930
            + V R+ VFEDS+R++ +   +  + +  + F  EEG D GG+ REW  ++S E+ NP Y LF+ S  D  T  I   S  NP+HL YF FVGR+I  A++    ++  FT  FYK +L KP+   D+E+ D   +K L++++ + +S +  + TFS E   FG+T+V +L  NG++I V+E  K+EYV+L  Q +    I +Q  +  +GF  +IP+ L+  F+E+ELEL++ G+  +D+ D K ++   +  A +  + WFW  + S+    +A+ +QFVTGSS+VPLQGF  L+   G  GP+ F +H  +   D LP A TCFN+LDLPAY +++ +   L +A++E     GFA
Sbjct: 1056 VHVRRENVFEDSFRELHRRSQEEWKNRFYIVFEGEEGQDAGGLLREWYVIISREIFNPMYALFKTSPGDKVTYMIYDQSQTNPNHLDYFKFVGRVIAKAIYDNKLLECYFTRSFYKHILAKPVKYTDMESEDYSFYKGLEFLIEHTVSDLGYELTFSTEIREFGVTEVRDLIPNGRNIAVSEDTKMEYVRLVCQMKMTSAIRKQLSAFLEGFYNIIPRRLISIFNEQELELLLSGLPNVDVDDLKANSEYHKYQANSLQIVWFWRALRSFDQTDKAKFMQFVTGSSKVPLQGFSALE---GMNGPQKFQIHRDDRSTDRLPSAHTCFNQLDLPAYETYDKLRSYLLKAIQECSEGFGFA 1411          
BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold22_size673200-snap-gene-2.20 (protein:Tk12673 transcript:maker-scaffold22_size673200-snap-gene-2.20-mRNA-1 annotation:"e3 ubiquitin-protein ligase hectd2")

HSP 1 Score: 163.696 bits (413), Expect = 1.063e-41
Identity = 113/400 (28.25%), Postives = 197/400 (49.25%), Query Frame = 0
Query:  553 LVAKIKVLRSXLNNXQP--QNGYCPLEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSED-VYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPI---------------NLDDIEAVDPDLHKSLKWILS--NDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAVE 932
            L A+  +++    + QP  +  +  + V R  + EDS ++I + + K ++KKL++ F  E GLD GG+ +EW  LL   +  P YG+F Y      Y   ++ +      +L  ++ +G ++G+AV++   +D  F    Y+ +L  P+                + D+  + PD+ +SL  +L    DI+  +  TFSV  E +G     +LK NG DI V   N+ E+V+LY+++     I ++F +   GF+ +   N L     +E+E ++ G  T D+ + K  T       + P +  FW +++ YS +++ + L+F TGS RVP+ G  D+     +   R+            LP A TCFN++ LP Y   + +  KLT A+    GF +E
Sbjct:  430 LTARRSLVQKMTRHQQPHIEIFFLNISVRRDHLVEDSLKEISEKQ-KDLKKKLKISFTGEPGLDMGGLTKEWFQLLIKSIFEPEYGMFVYYPHSRCYWFSLSKEG-----NLREYNLIGVLMGLAVYNSIILDLHFPPMCYRKLLTPPVVPANDGAPVGIVPSLTVSDLRDIMPDVTQSLANVLEYEGDIAEDLMLTFSVSVEEYGKVFTTDLKPNGDDITVTNENRKEFVQLYLEWLLNSTIRERFNAFYLGFHSVCASNALIMLRPEEVEQLVCGSQTFDVTELKKVTFYDGYKVDDPTIICFWEVLEDYSEDLQKKFLRFTTGSDRVPVGGTGDMTIKITRMKDRN----------HHLPIAHTCFNQIGLPDYKDKDRLKRKLTIAISNAEGFGLE 813          
BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold363_size195477-snap-gene-0.52 (protein:Tk06661 transcript:maker-scaffold363_size195477-snap-gene-0.52-mRNA-1 annotation:"ubiquitin-protein ligase e3c")

HSP 1 Score: 156.762 bits (395), Expect = 3.491e-39
Identity = 100/369 (27.10%), Postives = 189/369 (51.22%), Query Frame = 0
Query:  578 VSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRN-----EEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVN---PDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKP---INLDDIEAVDPDLHKSLKWILSNDISGIIDN--TFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIG-TIDIVDWKNHTRLKQ-CDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAV 931
            + R  ++ED++ ++       ++ K++V+  N     E G+D GG+ RE++        +P  G F+ + +     Q+ P+  VN   PD + ++ F+GR++G A++  H ++    + F   +L +    +++D + ++DP+L+K+L ++ + +   ++D    F++  E  G T+V+EL   G DIPV   N++EY+ +    +    I  Q  + ++G +++I  + L+ F  +EL+ +I G    I++ D +  T+     D + P ++ FW +V   S + +  LL+FVT  SR PL GFK+L+          F +       + LP A TC N L LP +     +  KL  A+E   GF +
Sbjct:  711 IRRNYIYEDAFEKLSPKNEPNLQLKMQVQLMNAVGLDEAGVDGGGLFREFMTEFLKTAFDPNRGFFRLTRDQ----QLYPNPTVNQIEPDFIPHYFFIGRMLGKALYENHLVELPLASFFLSKLLGQHSVNVDIDHLSSLDPELYKNLLYLKTYE-GDVVDLGLDFTMVSEEVGTTRVHELLPGGSDIPVTNENRIEYIHIVADHKLNRQIRAQCNAFRQGLSDVINLDWLRMFSYRELQTLISGAEHEINVDDLEQCTKYGNGFDEDHPTIKTFWKVVRELSEDQKRSLLKFVTSCSRPPLLGFKELEPP--------FAIQNVGDESERLPTASTCMNLLKLPEFKDERTLKTKLLYAIESGAGFEL 1066          
BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Match: snap_masked-scaffold347_size200506-processed-gene-0.2 (protein:Tk02315 transcript:snap_masked-scaffold347_size200506-processed-gene-0.2-mRNA-1 annotation:"ubiquitin-protein ligase e3b isoform x1")

HSP 1 Score: 127.102 bits (318), Expect = 5.397e-30
Identity = 101/377 (26.79%), Postives = 166/377 (44.03%), Query Frame = 0
Query:  576 LEVSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSSEDVYTLQINPDSAVNPDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWI--LSNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGT-IDIVDWKNHTRLKQCDAET-PVVQWFWAIVDS-YSSEMRARLLQFVTGSSRVPLQGFKDLQ----------------GSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEETCGFAV 931
            + + R  + ED YRQ+  +  K ++  +RVKF NE+GLD  G+ ++              G+F+   E+           +  DH S + ++                                 D++ ++D DL+KSL +I     D+S + D TFS + +  G    +EL   G+ I V  +NK+ Y+     FR    I+ Q  + +KGF+ +I  + L  F   E++ +I G    +D+ D + HT       ++  V+ W W I++  ++++ R+  L+FVT  S+ PL GF+ L+                G T  +  R F       PV+ LP A TCFN L LP Y     + EKL  A+    GF +
Sbjct:  704 ITIHRNRLVEDGYRQLSTLTSKALKGVIRVKFVNEQGLDEAGIDQD--------------GVFKEFLEETI------KRVLGKDHTSMYSYI---------------------------------DELPSLDADLYKSLTYIKHAGEDVSNL-DLTFSFDQDMMGKVVNHELLPGGRSICVTNANKINYIHKVAHFRMHFQIKDQLAAFKKGFSFVIKPDWLHLFSTPEVQRLISGDSAPLDLKDLRKHTHYYGGFHDSHRVIIWLWDILEKDFNAQERSAFLRFVTSCSKSPLLGFEYLEPPFSIRCVEVGDDEDDGDTLGSVVRGFLAIPRRDPVNRLPTASTCFNLLKLPNYHKKGTLKEKLKYAITSNTGFEL 1026          
BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold15_size728074-snap-gene-6.27 (protein:Tk08879 transcript:maker-scaffold15_size728074-snap-gene-6.27-mRNA-1 annotation:"e3 ubiquitin-protein ligase trip12 isoform x2")

HSP 1 Score: 123.25 bits (308), Expect = 1.504e-28
Identity = 113/412 (27.43%), Postives = 172/412 (41.75%), Query Frame = 0
Query:  578 VSRQEVFEDSYRQIVKMRYKVMRKKLRVKFRNEEGLDYGGVAREWLYLLSHEM----LNPYYG-LFQYSSEDVYTLQINPDSAVNPDHLS----------------------YFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLN--KPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEH-----------------------EAFGLTKVY------ELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQC------DAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVEE 925
            +SR  + + + + + ++ +   R  L +++ NE G   G    E+  L+S EM    LN + G   + SSEDV     N D  +   H S                       F F+G+ I  AV     ID  F+ PFY+ +L   +     D+  +DP +  ++       + GI+     +E                        E  GL          EL+++GK IPV   N  +YVKL   +    G+  Q  S+++GF  + P   L+ F   EL+ +  G      V W + T  + C      + E+  +Q+ + +V SYS E +   LQFVTG  R+P+ GFK L         + F     N P D LP   TC N L LP Y S +IM +KL  A +E
Sbjct: 1373 ISRDSLLKQAEQVVNELAHS--RSLLEIQYENEVGTGLGPTL-EFYTLVSKEMQRADLNLWKGDTVKISSEDVMDDDENADDCIEYVHSSTGLYPSPLARNIKSSHKVKVKNKFQFLGKFIAKAVLDNRMIDLPFSQPFYQWLLKAEQSFTTRDLMNIDPTIANTV-----TQLEGIVRKKRKLEEDDKITPNERLIQIKSLTMDGCPVEDLGLDFTLPGYPNIELRKSGKHIPVTLDNLHQYVKLVSHWMLIEGVSCQMESIREGFESVFPLESLQMFYPDELDQIFCGSMQGSFVQWDSKTLAETCKPDHGFNPESQGIQFLYEVVSSYSREEQREFLQFVTGCPRLPVGGFKSLSPPLTIVK-KTFDSPDVN-PDDYLPSVMTCVNYLKLPDYSSKKIMRDKLRVASQE 1774          
BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold114_size351134-snap-gene-1.12 (protein:Tk09543 transcript:maker-scaffold114_size351134-snap-gene-1.12-mRNA-1 annotation:"e3 ubiquitin-protein ligase hyd")

HSP 1 Score: 112.849 bits (281), Expect = 2.201e-25
Identity = 75/283 (26.50%), Postives = 129/283 (45.58%), Query Frame = 0
Query:  658 PDHLSYFHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWIL--------SNDISGIIDNTFSVEHEAFGLTKVYELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETP-----VVQWFWAIVDSYSSEMRARLLQFVTGSSRVPL--QGFKDLQGSTGQ-TGPRHFTLHLTNAPVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAVE 924
            P+ ++ F  VGR++G+ +         F     K +L + I   D+   DP +++SL+ ++        S  +   ++ TFS++          EL  NG+DI VN +N   YV+ Y Q+R     E+   +++ G  +++P   L     ++  L++ G+G I++    ++T       ETP       +W W IV+  S   +  L+ F TGS  +P    GF+ +   T +  G  H            LP A TC +RL +P Y S  I+  KL  A++
Sbjct: 1811 PERMNAFRNVGRLVGLCLLQNELCPLFFNRHVIKYLLGRSIRFHDLAFFDPVIYESLRQLVVDAENKDTSAQLFSALELTFSIDLSMEEGGGSAELVPNGRDIEVNAANVYNYVRKYSQYRMASCQEKALENMKLGLFDVLPAGSLDSLTAEDFRLLLNGVGDINVQTLISYTSFNDESGETPERLVNFKRWLWGIVEKMSPLEKQDLVYFWTGSPALPASEDGFQPMPSVTIRPAGDSH------------LPSANTCISRLYVPLYSSKAILKSKLLMAIK 2081          
BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Match: maker-scaffold732_size105256-snap-gene-0.22 (protein:Tk09377 transcript:maker-scaffold732_size105256-snap-gene-0.22-mRNA-1 annotation:"hypothetical protein SINV_14428")

HSP 1 Score: 102.834 bits (255), Expect = 1.326e-23
Identity = 74/265 (27.92%), Postives = 128/265 (48.30%), Query Frame = 0
Query:  664 FHFVGRIIGMAVFHGHFIDGCFTTPFYKMMLNKPINLDDIEAVDPDLHKSLKWILSNDISGIIDNTFSVEHEAFGLTKV--YELKENGKDIPVNESNKVEYVKLYVQFRWCLGIEQQFLSLQKGFNELIPQNLLKPFDEKELELVIGGIGTIDIVDWKNHTRLKQCDAETPVVQWFWAIVDSYSSEMRARLLQFVTGSSRVPLQGFKDLQGSTGQTGPRHFTLHLTNA---PVDSLPKARTCFNRLDLPAYPSFEIMAEKLTQAV 923
            F F+G ++G+A+  G  +      P +K++    +  +DI  +D D    L  I   D        F+V    F    V  +E+  + +   V   N+ EYVKL +Q+R     ++    +++G   ++P  LL  F   ELE+++ G   I +   K+    K  D  + +V+WFW +++ +S+  R+  L+FV G +R+P +   D +G       R F L + +    P + LP++ TCF  L +P Y   EI+ EKL  A+
Sbjct:   58 FRFLGLLMGIAIRSGSPLSLNLAEPMWKLLAGSKLTPNDITEIDKDYVPGLYCIRDMDPDS---KAFAVMDMPFSTPSVDGHEVNLSARYPKVTSENRGEYVKLALQYRLH-EFDEVVALVREGMARVVPVPLLSLFTGFELEMMVCGSPDIPLHLLKSVATYKGVDHNSNLVEWFWDVMEEFSNTERSLFLRFVWGRTRLP-RTIADFRG-------RDFVLQIMDKYHPPNEFLPESYTCFFLLKMPRYTCKEILREKLKYAI 310          
The following BLAST results are available for this feature:
BLAST of EMLSAG00000006171 vs. GO
Analysis Date: 2014-04-02 (Blast vs. GO)
Total hits: 25
Match NameE-valueIdentityDescription
-4.635e-5569.90symbol:lack "lethal with a checkpoint kinase" spec... [more]
-3.879e-9049.13symbol:smurf2 "SMAD specific E3 ubiquitin protein ... [more]
-3.796e-2266.01symbol:SMURF1 "Uncharacterized protein" species:96... [more]
-2.185e-4766.92symbol:smurf1 "SMAD specific E3 ubiquitin protein ... [more]
-1.016e-4466.26symbol:Smurf1 "SMAD specific E3 ubiquitin protein ... [more]
-4.913e-6668.05symbol:SMURF1 "E3 ubiquitin-protein ligase" specie... [more]
-3.771e-9148.64symbol:SMURF2 "E3 ubiquitin-protein ligase SMURF2"... [more]
-5.594e-7448.64symbol:Smurf2 "E3 ubiquitin-protein ligase" specie... [more]
-5.594e-7448.64symbol:Smurf2 "SMAD specific E3 ubiquitin protein ... [more]
-1.234e-4268.05symbol:SMURF1 "E3 ubiquitin-protein ligase" specie... [more]

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BLAST of EMLSAG00000006171 vs. C. finmarchicus
Analysis Date: 2014-05-09 (TblastN vs C. finmarchicus TSA)
Total hits: 25
Match NameE-valueIdentityDescription
gi|592796363|gb|GAXK01158205.1|1.059e-7975.00TSA: Calanus finmarchicus comp66507_c1_seq4 transc... [more]
gi|592796364|gb|GAXK01158204.1|1.071e-7975.00TSA: Calanus finmarchicus comp66507_c1_seq3 transc... [more]
gi|592796365|gb|GAXK01158203.1|1.089e-7975.00TSA: Calanus finmarchicus comp66507_c1_seq2 transc... [more]
gi|592796366|gb|GAXK01158202.1|1.101e-7975.00TSA: Calanus finmarchicus comp66507_c1_seq1 transc... [more]
gi|592943872|gb|GAXK01014681.1|7.336e-10348.58TSA: Calanus finmarchicus comp9879_c0_seq1 transcr... [more]
gi|592752837|gb|GAXK01201576.1|2.219e-10048.47TSA: Calanus finmarchicus comp87371_c0_seq1 transc... [more]
gi|592918907|gb|GAXK01039468.1|1.084e-9844.94TSA: Calanus finmarchicus comp718004_c0_seq4 trans... [more]
gi|592918909|gb|GAXK01039466.1|1.383e-9646.83TSA: Calanus finmarchicus comp718004_c0_seq2 trans... [more]
gi|592918910|gb|GAXK01039465.1|1.868e-9644.94TSA: Calanus finmarchicus comp718004_c0_seq1 trans... [more]
gi|592858480|gb|GAXK01099082.1|6.891e-8745.29TSA: Calanus finmarchicus comp239060_c0_seq1 trans... [more]

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BLAST of EMLSAG00000006171 vs. L. salmonis peptides
Analysis Date: 2014-05-10 (Blastp vs. self)
Total hits: 13
Match NameE-valueIdentityDescription
EMLSAP000000061710.000e+0100.00pep:novel supercontig:LSalAtl2s:LSalAtl2s336:65124... [more]
EMLSAP000000075149.630e-10947.94pep:novel supercontig:LSalAtl2s:LSalAtl2s431:6204:... [more]
EMLSAP000000110391.178e-10445.65pep:novel supercontig:LSalAtl2s:LSalAtl2s744:10937... [more]
EMLSAP000000049911.165e-7837.99pep:novel supercontig:LSalAtl2s:LSalAtl2s260:29995... [more]
EMLSAP000000034931.769e-4532.24pep:novel supercontig:LSalAtl2s:LSalAtl2s190:41856... [more]
EMLSAP000000088255.573e-4031.04pep:novel supercontig:LSalAtl2s:LSalAtl2s54:414186... [more]
EMLSAP000000094703.558e-2629.10pep:novel supercontig:LSalAtl2s:LSalAtl2s60:50964:... [more]
EMLSAP000000078087.173e-2526.69pep:novel supercontig:LSalAtl2s:LSalAtl2s458:20072... [more]
EMLSAP000000022455.431e-2124.25pep:novel supercontig:LSalAtl2s:LSalAtl2s1426:7958... [more]
EMLSAP000000002482.583e-1823.40pep:novel supercontig:LSalAtl2s:LSalAtl2s1036:6887... [more]

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BLAST of EMLSAG00000006171 vs. SwissProt
Analysis Date: 2017-02-10 (Blastp vs. SwissProt)
Total hits: 25
Match NameE-valueIdentityDescription
gi|73919319|sp|Q9V853.3|SMUF1_DROME3.624e-5569.90RecName: Full=E3 ubiquitin-protein ligase Smurf1; ... [more]
gi|218547424|sp|A9JRZ0.1|SMUF2_DANRE2.428e-9149.13RecName: Full=E3 ubiquitin-protein ligase SMURF2; ... [more]
gi|85681896|sp|Q9CUN6.2|SMUF1_MOUSE7.660e-4566.26RecName: Full=E3 ubiquitin-protein ligase SMURF1; ... [more]
gi|123896339|sp|Q2TAS2.1|SMUF2_XENLA1.234e-9048.57RecName: Full=E3 ubiquitin-protein ligase SMURF2; ... [more]
gi|17865624|sp|Q9HAU4.1|SMUF2_HUMAN2.246e-9248.64RecName: Full=E3 ubiquitin-protein ligase SMURF2; ... [more]
gi|17865625|sp|Q9HCE7.2|SMUF1_HUMAN3.177e-6766.01RecName: Full=E3 ubiquitin-protein ligase SMURF1; ... [more]
gi|17865628|sp|Q9PUN2.1|SMUF1_XENLA8.054e-4467.86RecName: Full=E3 ubiquitin-protein ligase SMURF1; ... [more]
gi|218547425|sp|A2A5Z6.1|SMUF2_MOUSE6.384e-9249.67RecName: Full=E3 ubiquitin-protein ligase SMURF2; ... [more]
gi|74598276|sp|Q5BDP1.1|RSP5_EMENI1.386e-13840.35RecName: Full=E3 ubiquitin-protein ligase RSP5; Al... [more]
gi|300681225|sp|Q4WTF3.2|RSP5_ASPFU1.030e-13739.62RecName: Full=Probable E3 ubiquitin-protein ligase... [more]

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BLAST of EMLSAG00000006171 vs. Select Arthropod Genomes
Analysis Date: 2017-02-20 (Blastp vs. Selected Arthropods)
Total hits: 25
Match NameE-valueIdentityDescription
EEB15624.10.000e+046.49E3 ubiquitin-protein ligase Smurf1, putative [Pedi... [more]
XP_006564410.11.978e-9173.33PREDICTED: E3 ubiquitin-protein ligase SMURF2 isof... [more]
EFX80702.11.497e-1866.74hypothetical protein DAPPUDRAFT_318414 [Daphnia pu... [more]
gb|KFM66753.1|2.224e-2261.29E3 ubiquitin-protein ligase SMURF2, partial [Stego... [more]
AGB93593.11.756e-5569.90SMAD specific E3 ubiquitin protein ligase, isoform... [more]
AAF57824.31.756e-5569.90SMAD specific E3 ubiquitin protein ligase, isoform... [more]
gb|EFA08997.2|1.176e-5970.69E3 ubiquitin-protein ligase Smurf1-like Protein [T... [more]
EAA05201.46.605e-6362.56AGAP011242-PA [Anopheles gambiae str. PEST][more]
gb|KPM09387.1|1.571e-16270.03E3 ubiquitin-protein ligase SMURF2-like protein [S... [more]
XP_006571319.11.478e-12039.30PREDICTED: E3 ubiquitin-protein ligase NEDD4 isofo... [more]

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BLAST of EMLSAG00000006171 vs. nr
Analysis Date: 2017-02-20 (Blastp vs. NR (2/2017))
Total hits: 25
Match NameE-valueIdentityDescription
gi|954547610|ref|XP_014616351.1|0.000e+050.86PREDICTED: E3 ubiquitin-protein ligase SMURF2 isof... [more]
gi|954547606|ref|XP_014616349.1|0.000e+050.86PREDICTED: E3 ubiquitin-protein ligase SMURF1 isof... [more]
gi|972174080|ref|XP_015179970.1|0.000e+050.40PREDICTED: E3 ubiquitin-protein ligase SMURF1 [Pol... [more]
gi|242015442|ref|XP_002428362.1|0.000e+046.49E3 ubiquitin-protein ligase Smurf1, putative [Pedi... [more]
gi|1000769969|ref|XP_015608168.1|5.285e-6155.15PREDICTED: E3 ubiquitin-protein ligase SMURF2 [Cep... [more]
gi|347602183|gb|AEP16400.1|0.000e+041.94SMAD specific E3 ubiquitin protein ligase [Mnemiop... [more]
gi|1121118511|ref|XP_019548334.1|2.021e-5653.14PREDICTED: E3 ubiquitin-protein ligase SMURF2-like... [more]
gi|1058258900|gb|JAT32935.1|3.975e-4152.48hypothetical protein g.48621, partial [Graphocepha... [more]
gi|1058005834|gb|JAS06601.1|2.265e-9553.46hypothetical protein g.17677 [Clastoptera arizonan... [more]
gi|939237793|ref|XP_014261551.1|9.983e-7052.85PREDICTED: E3 ubiquitin-protein ligase SMURF2 [Cim... [more]

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BLAST of EMLSAG00000006171 vs. Tigriopus kingsejongenis genes
Analysis Date: 2018-04-18 (Blastp vs. Tigriopus kingsejongensis proteins)
Total hits: 12
Match NameE-valueIdentityDescription
maker-scaffold56_size446035-snap-gene-3.360.000e+077.12protein:Tk11506 transcript:maker-scaffold56_size44... [more]
snap_masked-scaffold186_size273091-processed-gene-0.46.641e-11339.03protein:Tk00902 transcript:snap_masked-scaffold186... [more]
maker-scaffold125_size330479-snap-gene-2.152.288e-10330.84protein:Tk06892 transcript:maker-scaffold125_size3... [more]
snap_masked-scaffold450_size166944-processed-gene-0.51.075e-8138.44protein:Tk00124 transcript:snap_masked-scaffold450... [more]
maker-scaffold22_size673200-snap-gene-2.201.063e-4128.25protein:Tk12673 transcript:maker-scaffold22_size67... [more]
maker-scaffold363_size195477-snap-gene-0.523.491e-3927.10protein:Tk06661 transcript:maker-scaffold363_size1... [more]
snap_masked-scaffold347_size200506-processed-gene-0.25.397e-3026.79protein:Tk02315 transcript:snap_masked-scaffold347... [more]
maker-scaffold15_size728074-snap-gene-6.271.504e-2827.43protein:Tk08879 transcript:maker-scaffold15_size72... [more]
maker-scaffold114_size351134-snap-gene-1.122.201e-2526.50protein:Tk09543 transcript:maker-scaffold114_size3... [more]
maker-scaffold732_size105256-snap-gene-0.221.326e-2327.92protein:Tk09377 transcript:maker-scaffold732_size1... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
LSalAtl2s336supercontigLSalAtl2s336:651240..657086 +
Analyses
This gene is derived from or has results from the following analyses
Analysis NameDate Performed
ensembl2013-09-26 .965016
Blast vs. GO2014-04-02
TblastN vs C. finmarchicus TSA2014-05-09
Blastp vs. self2014-05-10
Blastp vs. SwissProt2017-02-10
Blastp vs. Selected Arthropods2017-02-20
Blastp vs. NR (2/2017)2017-02-20
Blastp vs. Tigriopus kingsejongensis proteins2018-04-18
Properties
Property NameValue
Logic nameensemblgenomes
Descriptionmaker-LSalAtl2s336-augustus-gene-6.36
Biotypeprotein_coding
EvidenceIEA
NoteE3 ubiquitin-protein ligase Smurf1
Cross References
External references for this gene
DatabaseAccession
Ensembl Metazoa (gene)EMLSAG00000006171 (primary cross-reference)
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameSpeciesType
EMLSAT00000006171EMLSAT00000006171-702018Lepeophtheirus salmonismRNA


Sequences
The following sequences are available for this feature:

gene from alignment at LSalAtl2s336:651240..657086+

Legend: mRNA
Hold the cursor over a type above to highlight its positions in the sequence below.
>EMLSAG00000006171-688937 ID=EMLSAG00000006171-688937|Name=EMLSAG00000006171|organism=Lepeophtheirus salmonis|type=gene|length=5847bp|location=Sequence derived from alignment at LSalAtl2s336:651240..657086+ (Lepeophtheirus salmonis)
TGTTATTTAATATTTTGTATGCGACAGCTGTAACTGGAATTAAGAGGTAG CAGGAGAGGAGGAGGAGACGCAAGGTAGTACTAAGATTGGGCAAGCATTT CTCCCTTTTTCTTAATAATACTATTTTGACTGTACTTGGGAGCACCTTGA CTTAAGAGTCAATCATTTTTGTTTCGCCCCATTGTCGTAACTAGCTAGTA ACCCCCTCCTCCTCCTCCTCCTCCTTTTCCTTCATTGAGCTTGCACACTA CTACTAGCTGTACTGGTCTGTGTCTGTATGAAATAGCTGCTGCTAGCTGT AGCGTAGTGGAGTGGAGCTGAGACGTACATACTTGCATGAAGATTAGCTC ATCGTCACTCAGATTTAACATTAGTGTTTTTCTAATACTAAATATAAAAT AGTACATACTCGTCAATGTTTAAGCTGGTGATATGAATAAATAGTGACTG TCATGGAGATAAGAGGGACAAAGATTCGATTAACGATACTTTGCGCCCGT AATTTAGCCAAAAGAGATTTATTTCGTCTTCCAGATCCATTTGCTCGAGT GGATGTGGATGCAACAGGRGTTACGCATGTCTCAGAAGCTGCCAAAAACA CGACGGATCCCAAGTGGAACACGCATTATGATCTGCATCTCAGTCCCAAT GAATCCTTCACACTCTCCGTTTGGAATGATAAAAAGGTAGAGCTAGGAGA AGAAGGGTACTTCTCATTCTTGTCTATTCATAAACTGACTTACTCTAATC ACTTCATTTATAGTTAGTAGTTTCTCCTACTTCGAATGACTTAGTCTAGT AGAGAAGTCGAGATCATGAGGGATCCCATCAATTATTGAATATTAGTTTT TATCACTTGTTTCTTTATTAAATATTATTACTGCTACTTGAGGAACTCAA TACCCTTGTAATTTGAGCCGATCCTGTTCGCCTCTCCTTTTATGTCTTCT TTTAATTACGATTATGCAACTCAAGAAAACAAAAAGTAAAACTATTCATA CCTTATCTAATACTTTTTTTATATTTCCTACCTTCTATTTTATTGTATTT TTGCTCATGAGGGTTATTGTTCCTCCATGAGGAGGATGGTCATAATAATA ATACCGAATACCTTCCTTTATTTTGCAGATGCACAAAAAAACGTATAACT CCGGTTTCCTGGGATGTGTAAGACTCATGGGAAATGCAATTCAAAGAATC AAAGACACAGGCTATCAAAGACTGGATTTAGTATCAGATACTAAAATTGG TTTGCCGGTTAAAGGACAAATAGTGATATCTTTACTCTCCAGAGACGGTC ATGGTACAGGTTCGTTCATTTTCCTTGTCCTTCCTCTCAATTAATTGGAT TTTTTTTATCATATAGGAAGTCTAAATGCTGTGGTGGATACTTTAGGCAA TTTGACTTGTTCAAATGACTTACCTGAAGGGTGGGAGGAGCGTCGAACTC CATCTGGGCGAGTCTATTACGTCAATCATGTCAGAAGAACAACTCAATGG GCTAGACCAACTGAGCCAGCTTCTTGTGGTACAACTCGACGGGGTGACCG TGGAGGAACGTTATCCATAACCAATAGTAGTAAAAACAATAATCTTTCAA AAACTACTACAACGACGACGMCGYCTTCCACTCCAACATCTGAAGGCGCC AATTCTCTTCCAATAGCTTCCTCTCATGCCTTAACCGGAAATGGGACTGA CAACCTTCCGGCTGTGAATAATCAAGATTCTCCGTCGTCCCTCTCTGATC CCTTTTCATCTCTACAACCAAGAAGACATCAAATCACTCAACCAGACAAT CTTCCTCCGGGATATATTATGAAAACCACCGAGCATGGACAGATTTATTA CATACACGAAGGAACGGGTAATATATTAGATAATTGAAATGAAACAATTG ATGGGTGATTTTTCTTTTTTTGTCAAATGCACATTAGGCCAAACGACATG GCATGATCCTAGACTTACAAGGGCTCTTACGTCAAATGAAGAATTGAATT TAGGACCGTTACCTCCGGGCTGGGAACAAAGGGTTACAGACTCAAGCCGA CCTTATTTCGTTAATCACAATAATCATACTACTCAGTTCTCAGACCCCAG AATCCCCTTAATGAATGACGCTAACAATCATCATCATGATGATAATTCTC CTACAAAYCCCTCTCGCGGGACTTCCAAAAATGAGYCAGCCACTAATGTA TTGCCAACAAATCCTTCTTTGTCTCCTGAAACCAATGATACCCACTCTAA CGTGAGAAATAGTGAGCCTGCTGATAGCCCTAGTACTTCAAACGAGGCAC CCCTTAGTGTAACGTGTTCGTCCTCAGGTTCTACTTCTACAACAACCTCA TCAACTCCTTCTTCATTGGCMCCATCCAATATTACTAACACTACTGTTTC CAATGTCAGAGCAACAACATCAAATAATACAGTACTTCGTCCCAGAGGTG AAGTTATTCAACAACTATCTCCTTTTCCTGTTCTCCCGGTCTCTTCGTCA TCATCTATCATTTCATCGCCCCCACCGCCACCCCCGCCACAGACAACATC ATCGTCCTCTTCATCAGTTCCTAATACTCCACCCGTTATTGGGGGTCCTA GGTGTTGGCCTCAGGATCCCTTATCAATTGCGTTACATGGTGGTGGGACT GAGTTTCTGCCCAAGTTTAAAAAAGACCTAGTAGCTAAAATAAAGGTACT AAGGTCAGASCTCAATAAYCYCCAGCCACAAAATGGGTATTGTCCTCTTG AAGTAAGTCGACAGGAGGTTTTTGAGGACTCTTATCGGCAAATAGTTAAA ATGAGATATAAAGTCATGCGCAAAAAGCTCAGAGTCAAGTTCCGTAACGA GGAAGGATTGGATTATGGTGGAGTCGCTCGAGAATGGTTATATCTTTTAA GTCACGAAATGTTAAATCCATACTATGGACTTTTCCAATACTCGAGTGAG GATGTGTACACACTTCAAATCAATCCCGACTCTGCAGTGAACCCTGATCA CTTAAGTTATTTTCATTTTGTGGGAAGAATAATTGGCATGGCTGTTTTTC ATGGACACTTTATTGATGGTTGCTTCACAACTCCGTTCTATAAAATGATG TTAAACAAACCCATCAATCTAGATGACATTGAGGCTGTTGATCCAGATCT TCATAAGTCTCTCAAATGGATTTTATCGAACGATATATCGGGAATTATCG ACAATACTTTTAGTGTGGAGCACGAGGCCTTTGGATTGACGAAAGTATAT GAATTAAAGGAGAATGGAAAAGACATTCCTGTAAATGAGAGTAATAAAGT CGAGTACGTCAAACTCTACGTACAGTTTAGATGGTGTCTTGGAATTGAGC AGCAGTTTCTATCTCTTCAAAAAGGTTTTAATGAACTAATTCCCCAGAAT CTACTCAAACCTTTTGATGAAAAAGAGTTGGAGCTTGTGATTGGTGGGAT TGGTACGATTGATATTGTGGACTGGAAAAATCATACTCGTCTTAAGCAAT GCGATGCAGAAACACCTGTTGTACAGTGGTTTTGGGCCATCGTGGATTCA TACAGTAGTGAAATGAGAGCACGTCTTCTTCAATTTGTTACAGGGAGCTC TAGAGTCCCACTCCAGGGATTTAAGGACTTACAAGGTAGTACAGGACAAA CCGGACCACGGCATTTTACCTTACATCTCACAAATGCTCCTGTGGACAGT CTACCAAAGGCACGCACTTGTTTTAATCGTCTTGATCTTCCTGCCTATCC TTCTTTTGAAATAATGGCTGAAAAACTTACTCAAGCAGTAGAAGAAACTT GTGGATTTGCTGTTGAATAAAAAAATAAATGCTAAATATCCCTCCTCCAC CGCCCCTTCTTGTATCTTAGTTAAAATATTTTAAAAAATACGTCCTCCGT TTTTTTTTTGGGCTTTTTTTTTTTTTACAAAAAGATACTAGTTAAAAAGC AAAAAAAAAAAAATAATCTTTTTTTATATAGATTCTAGTGTATTTATTTT GTGGGTGCTTATATTCAGCAGATCTATTCTATATAGTTTCCAAAAATCTG TGTAAAGTAAGTTTATAAATATAAGTCAAGATATAACATATTATATTTTA TTTTAACAATTTTTTTTTAGTTCCAAAGGAAAGACACTAAATATTGAATG ACCTTTCTATAAATATAATAGGAACTTAAAGATATTTCCCTGTTTTTATG TTACCTAAAATGTTATAAATAATCCAGATTGTTATTCCAATTCCGTTGAC ACTCCCACCCCTTCTCTCCTCCCTAGTCTATGCCCCTTCATATGAAGAAC CAGGGACAGTTTAATATATTTTTTTAAATGATAAAAACGAAAGATAAAAT CAGAAGGCGAATGGGGAGGAGGGCTCACCCTCTGATCCTCATAGTCTCAA TTCGATTATTCCATGTGTTATTATTAACTCATTCATTTATTCAMCCGCCA TTCCTAATGGCGATATTGGCGTTGTAGCTTGTGATTTTTCATCGTGATGC CGATTTCTACTAGACTTGTGAGGCCAAAATATACAGTTTAAAAAACGAAA CAAAAAATCAACCCCCTTTCCCATCTTATTGCTTGTCAAAATGCTATTTT TCAAAAACTAGAAAAATATACATAAATATATATCTACAAATACCTGATGG TCCATAGTAACTATATTTTTTTAATTTAAATAAGAACGTATGTGTGTATT GGATTCAACTTCTAAGTACACGGGTAATAATCCATTCGTGAGTAATCGAT AAAATTTATAACTTTTCTACTATATTTAGTTTCTATTCTTTAAAAACAAT TACTTAAAACAAAAGACTGAGGAAACTAAGATAAATTATGCGGCAGAATG GATGATTTCGGAATATTTTTTAATTTTGGATTCGATGGACATGTCAACAA ACTTGTCTCCAATGGATACAACCATTCCACCAAGGATTGAGGGATCCACC TTGTAGGTTACAAGGCTCTTTTGTCCCGCCTTTAAAAACTTMCCAATGGC TGCCTCAACCTCCTTTGTTTTGACGGCATCCAATTTCTGAGGTGAATAAT ATACATAATAATGAATATGGATCACTCCTAATGAAAGGATGATGCTATTA CCTTTGCCGTAGTGACTTCGCAGACAACTTCGCCACGGTGTGCAGCCATA ATGGTACTGAAGGAACCAATAACGTCATTGATATTGGTGTACCGTCCATT TTCAGCCAATGCAAGCATGCAGTTCTTGGAGAGCGCATTCATTTTCAACT TGTCACAAACGGATGACAATCCTTCAAACTTGAGTGTTTTTTTAATGGTG GGATCGTCCAAAAACTCCTTTAAGCGTAGATCCTTATCCATGACGACCTT GAAGCCCATGAGATCCTTTTCAACAGTAGGAAGCGATTTTTGCTTGGAAG CGGCAGAGTAGAGAGCTGTGGCATATCGACCCTCGGTTCCGTATACATAG ACTGGAGGCTTGACGAGAGCACTGCGAGAGGAGGAGCTGCTAAGTCCTCG AGTCAAAATATTCAAATTCCTAGGAGATATTGTTACTCATTGTGTTTTAC AGGGAACTTGAATTGAACTTACTTTACGGCAGACATAATTTGGATTTCCC AGAGAATAATGTTTAAGTAAATATAGAGAGAGAAGTCTGATCAATCGATG GAATCAAGATGGAACGTCTCAGTTAGCTGTAGGTGGAAGGTTGAGTGATA TTTAACAGGGCAGCTGCGCAAAGGCTCCCCTTTTCTTCAGCATCAGCTGA TCAGTGTTTTCATCTCAATATTACTAATAGAACAATTGATTTCATCAACA CAATTATCCCAGTTGTTCCATCAAGAGTTTGTCGTTTTAGAACTCCG
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