BLAST of vacuolar protein sorting-associated protein 33a vs. L. salmonis genes
Match: EMLSAG00000000255 (supercontig:LSalAtl2s:LSalAtl2s1037:40015:45241:1 gene:EMLSAG00000000255 transcript:EMLSAT00000000255 description:"augustus_masked-LSalAtl2s1037-processed-gene-0.2")

HSP 1 Score: 676.396 bits (1744), Expect = 0.000e+0
Identity = 327/606 (53.96%), Postives = 468/606 (77.23%), Query Frame = 0

Query:   10 QFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVI---SKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSR--NYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHP--GWKTIKDVLDLLPGPTLEEAQKT--SSNVKRIKPPGED-HQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLLTHVDDNLAP 605
             F  +RDL+++EL + LDKYAG+K++VWD+ L  PL LIA++  L++R V+KM+PLK G L ++    EN+VF+TRP V LM+ VADNVK   +S  +R  FHI+F+P++SLLCEMRLK+K V+GSFTFLD+LS  WFPL+TDVISME+  +F +++LN DPT L+ VAK ++ALQ++ GIIP+V GKG AA++V+++MT++RKE   +E  ++   S IDTL+IIDRQID +SP  TQLTYEGLIDE++SI + +VK+PA++F   +       +   MK   LNS +EL++E+RDKNFNA+G  ++KKA+ ++AQ +ERH+AK+++E++ FV+K+P+M+ ++ SL  HT+IAE +++ TN   FL+ L++EQEL+N+QN D+  E +ED  C + DI+ VLRL+C+Q+ + SG+KPK+LESYR+LILQ YGY+HLL+L NL++ GLL P +  S   NY VLRK+LNLT DDV+EQ P DIAYV+SVYAP+SIRLVQ+L+    GW++I+DVLD+LPGPTLEE Q +   S  K I     D + STTLV+FVGG TFAEI+ALRYL+Q+E+S T+Y++ATT+I+NG+ F++S+++++ +  AP
Sbjct:   15 HFSCIRDLSKKELSECLDKYAGSKVIVWDEALIGPLGLIAEYQFLRDRHVKKMIPLKLGPLKLE-ENAENIVFITRPLVPLMDIVADNVK---NSEDSRKAFHILFLPRKSLLCEMRLKDKGVFGSFTFLDQLSASWFPLETDVISMEDSSIFSEYYLNGDPTSLHSVAKALMALQSIVGIIPKVYGKGKAAKQVYDYMTRLRKEAADEESNLLPSDSSIDTLVIIDRQIDSLSPLATQLTYEGLIDEIYSIKHASVKIPADRFEPNEKDKVLSVSEKEMKQCFLNSNEELFAEIRDKNFNAVGHVINKKAKWMAAQFNERHEAKSIKEIKSFVEKLPQMQAIKTSLMNHTSIAESIRDWTNRTEFLDALQIEQELMNYQNVDKLHESIEDLICANADILRVLRLICIQSFITSGLKPKILESYRKLILQNYGYQHLLSLLNLEKAGLLYPMVNGSNKSNYVVLRKKLNLTLDDVNEQDPKDIAYVYSVYAPISIRLVQHLSFSPGGWRSIRDVLDILPGPTLEEKQNSMGKSKPKLITTNNVDSNNSTTLVVFVGGVTFAEISALRYLAQKEESSTDYLIATTSIINGNNFLESMMSNIKEG-AP 615          

BLAST of vacuolar protein sorting-associated protein 33a vs. L. salmonis genes
Match: EMLSAG00000002778 (supercontig:LSalAtl2s:LSalAtl2s15:983432:989749:1 gene:EMLSAG00000002778 transcript:EMLSAT00000002778 description:"augustus_masked-LSalAtl2s15-processed-gene-9.2")

HSP 1 Score: 159.458 bits (402), Expect = 2.908e-42
Identity = 150/600 (25.00%), Postives = 284/600 (47.33%), Query Frame = 0

Query:   14 LRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEH----SHGTRAEFHIMFVPKRSLLCEMR--LKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVV---DEPQVI-SKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFV-DKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLEN-LKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSR-NYPVLRKRLNL---TRDDVDEQQPNDIAYV-HSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            L  L+   L + L++  G K L+ D  L  PLD +A  S LK   V+K+   +    P           +    V+L+ +   N K   H       +  EFHI+F+P+  +L  +R   +E+ VY    +L        P D D+ S+E   +F    LN D + L  V+  ++++++++G I   +  G  A  V        K+L V     PQ   S  +   I+DR ID +S  L+ LTYE L+DE+F IN  +++L           DS       +  + L+++D+ ++++R+K+   I   LS  AR +  Q  +R +  +V E++ FV + +  ++   ++LS+H   +E++++   +  F E+ L +E  ++  Q     ++ +E+       +  V+RL+ L ++ ++G      ES RR  +Q YG++ +  L+ L + GL+  +    + ++     ++NL   +++ V   +P+D +YV +  Y PL  R+V++    G +T            +      S+ ++    P  +     L+ F+GG T AE+A+L+ L  +      +++A T+   G   ++S+L
Sbjct:   12 LVQLSCENLRNVLNQIHGNKDLIIDPTLMKPLDTVAGMSILKSLGVKKIFKFETKACPS----------IAYSRVYLLSSSLINAKDVIHHFRDKKTSEGEFHIIFLPR--ILHSIRELFEEEGVYDQI-YLHNYMWELIPFDEDLFSLELPCLFSQVFLNGDSSLLPSVSYSLMSIESLYGKITNKVAIGSHAVNVL-------KQLDVISPSTPQTFNSYFEYAFIMDRDIDYVSTLLSPLTYESLLDEVFGINCGSIEL-----------DSRVTTSDKVVKLQLSNKDKTFTKIRNKHIGDIFSYLSVHARQLK-QHQKRAENLSVSEMKNFVQNNLKDIQNQYKALSLHIGASEVIQK--EKGSFFESMLHIEHGIVRGQCLKEAIQIIEELMAQLCSMDIVIRLMALASICHNGFSSGDYESLRRQFVQAYGFEKMHILRTLQKRGLVFKKDSNRKHSFQTFATKVNLIPKSQESVSLTEPSDPSYVFNGAYTPLISRIVESFMGKGNQT----------EFMSHLTHLSTRIQFQNKP--NRSKFVLIYFIGGYTLAEVASLKIL--QTIMGHHFVIAGTSNTTGKKIMESVL 563          

BLAST of vacuolar protein sorting-associated protein 33a vs. L. salmonis genes
Match: EMLSAG00000000458 (supercontig:LSalAtl2s:LSalAtl2s106:529291:531005:-1 gene:EMLSAG00000000458 transcript:EMLSAT00000000458 description:"maker-LSalAtl2s106-augustus-gene-5.13")

HSP 1 Score: 54.299 bits (129), Expect = 5.681e-8
Identity = 111/599 (18.53%), Postives = 223/599 (37.23%), Query Frame = 0

Query:   14 LRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQV---EKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVK--FEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADR-----------PLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            L DL R+ L D LD   G K L+ D      +DL    S + +++V   E +   + GR      +Y++        + ++  V +N++    E S+ +   +H+ F  +   +   RL E ++      + E+ +   PL  +V  ++  +    F  N     + + ++G+ A   +  +    I     + +       +   L        S    ++I+DR+ D I+P +   TY  LI E+   N N V          DSS       F          DE Y +    ++     S+ +       + +E  +  ++ +++ F+ + P+ K L   +  H +I + + +    +  L    +EQE++   N              P    E+      D+  +L +  L+                   L+   +   +   +++   +        RN+ + +K   +    V     N+   V +VY     RL            KDVL+ +    +        N+ R        +   LV  VGG T +EIA +  L++ + +    +V  T+++N + F+K ++
Sbjct:    5 LPDLIRQSLFDELDAIKGMKTLIMDSVTMGIIDLACSKSLIMKKEVFLFEDVTKFRQGR-----KRYKDPKLSHLKAIVVIRNVNENIEALIYELSNNSYNSYHLFFTNRIDSMTIKRLAEADMNEVVKGVKEIYMDIEPLLNNVFMLKPLEGVTKF--NYKSADIKRFSEGLSAATKLLRMTSSSILYQSTSLETDRIAHSLSSSLESSNG--FSDEGVILILDRRFDPITPLVFNWTYFNLIHELLGFNNNKVTF-------TDSSSKTTQTSFS------TDGDEFYKQNIYADYGKFCSSIKRLVDDFQKRRNEHDNMDSLGDIKDFISRYPEFKKLSGIVDKHVSIVDEIAKKVQMRDLLNISLLEQEILVSSNQSSIVNKLKRDLFPPPSQAENTNIQRSDLFRMLLITALK-------------------LKDISFLSQIQASDVNTADISCINEYIGRNFELFQKDNRIY--PVSSNIINEFKTVENVYTRYQPRL------------KDVLNAVINGDIHHL-----NLNRYGNYSPSKK--ILVFVVGGLTCSEIALVHDLNKEQRA--RIVVGGTSLLNSECFMKDII 539          

BLAST of vacuolar protein sorting-associated protein 33a vs. SwissProt
Match: gi|23396917|sp|Q96AX1.1|VP33A_HUMAN (RecName: Full=Vacuolar protein sorting-associated protein 33A; Short=hVPS33A)

HSP 1 Score: 592.038 bits (1525), Expect = 0.000e+0
Identity = 289/592 (48.82%), Postives = 406/592 (68.58%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            S   +  ++LR+  RREL + LDK AG+K +VWD+ LT P  LIA++S LKE +VEKM  LK  RLP      +N++F  RP + LM+ +A+NV  E+    TR +FHI+FVP+RSLLCE RLK+  V GSF   +E SL   P D D++SME+   F++ +L  D T L   AKG++ LQA++G IP++ GKG  AR+V   M +M++E    +  +    D L+++DR +DL++P  TQLTYEGLIDE++ I  + VKLP EKF      D  +  P   K + LNS +ELY+E+RDKNFNA+G  LSKKA+ +SA  +ERH+AKTV E++ FV ++P M+  R SL+ HT+IAEL+K+ T  + F + L VEQE ++  + D+   Y+ED       ++ VLRLVCLQ+V NSG+K KVL+ Y+R ILQTYGY+H+LTL NL++ GLL PQ     NYP +RK L L  DDV+EQ P DI+YV+S YAPLS+RL Q L+ PGW++I++VL +LPGP  EE Q   + +++ + PGE+    TL+ F+GG TFAEIAALR+LSQ ED  TEY++ATT ++NG +++++L+
Sbjct:    6 SYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKGNRLPA--ADVKNIIFFVRPRLELMDIIAENVLSEDRRGPTR-DFHILFVPRRSLLCEQRLKDLGVLGSFIHREEYSLDLIPFDGDLLSMESEGAFKECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMIRMKREFTGSQNSIFPVFDNLLLLDRNVDLLTPLATQLTYEGLIDEIYGIQNSYVKLPPEKFAPKKQGDGGKDLPTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARGSLANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVNNYIEDCIAQKHSLIKVLRLVCLQSVCNSGLKQKVLDYYKREILQTYGYEHILTLHNLEKAGLLKPQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQPLPTGLQKKRQPGENR--VTLIFFLGGVTFAEIAALRFLSQLEDGGTEYVIATTKLMNGTSWIEALM 592          

BLAST of vacuolar protein sorting-associated protein 33a vs. SwissProt
Match: gi|23396893|sp|Q63615.1|VP33A_RAT (RecName: Full=Vacuolar protein sorting-associated protein 33A; Short=r-vps33a)

HSP 1 Score: 586.26 bits (1510), Expect = 0.000e+0
Identity = 290/594 (48.82%), Postives = 407/594 (68.52%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPN--PFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            S   +  ++LR+  RREL + LDK AG+K +VWD+ LT P  LIA++S LKE +VEKM  LK  RLP      +N++F+ RP + LM+ +A+NV  E+    TR +FHI+FVP+RSLLCE RLK+  V GSF + +E SL   P D D++SME+   F++ +L  D T L   AKG++ LQA++G IP++ GKG  AR+V   M +M++E    +  V    D L+++DR +DL++P  +QLTYEGLIDE++ I  + VKLP EKF AP           P   K + LNS +ELY+E+RDKNFNA+G  LSKKA+ +SA  +ERH+AKTV E++ FV ++P M+  R SL+ HT+IAEL+K+ T  + F + L VEQE ++  + D+   Y+ED       ++ VLRLVCLQ++ NSG+K KVL+ Y+R ILQTYGY+H+LTL NL++ GLL  Q     NYP +RK L L  DDV+EQ P DI+YV+S YAPLS+RL Q L+ PGW++I++VL +LPGP  EE Q   + V++ + PGE+    TLV F+GG TFAEIAALR+LSQ ED  TEY++ATT ++NG +++++L+
Sbjct:    6 SYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKGSRLPA--ADVKNIIFLVRPRLELMDMIAENVLSEDRRGPTR-DFHILFVPRRSLLCEQRLKDLGVLGSFIYREEYSLDLIPFDGDLLSMESESAFKECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMVRMKREFTGSQNSVFPVFDNLLLLDRNVDLLTPLASQLTYEGLIDEIYGIQNSYVKLPPEKF-APKKQGGGGGKDLPTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARGSLANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVNNYIEDCIAQKHPLIKVLRLVCLQSMCNSGLKQKVLDYYKREILQTYGYEHILTLNNLEKAGLLKAQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQPLPTGVQKKRQPGENR--VTLVFFLGGVTFAEIAALRFLSQLEDGGTEYVIATTKLINGSSWLEALM 593          

BLAST of vacuolar protein sorting-associated protein 33a vs. SwissProt
Match: gi|342187018|sp|Q9D2N9.2|VP33A_MOUSE (RecName: Full=Vacuolar protein sorting-associated protein 33A)

HSP 1 Score: 583.178 bits (1502), Expect = 0.000e+0
Identity = 291/595 (48.91%), Postives = 407/595 (68.40%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPN---PFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            S   +  ++LR+  RREL + LDK AG+K +VWD+ LT P  LIA++S LKE +VEKM  LK  RLP      +N++F+ RP + LM+ +A+NV  E+    TR +FHI+FVP+RSLLCE RLK+  V GSF   +E SL   P D D++SME+   F++ +L  D T L   AKG++ LQA++G IP++ GKG  AR+V   M +M++E    +  V    D L+++DR +DL++P  +QLTYEGLIDE++ I  + VKLP EKF AP            P   K + LNS +ELY+E+RDKNFNA+G  LSKKA+ +SA  +ERH+AKTV E++ FV ++P M+  R SL+ HT+IAEL+K+ T  + F + L VEQE ++  + D+   Y+ED       ++ VLRLVCLQ+V NSG+K KVL+ YRR ILQTYGY+H+LTL NL++ GLL  Q     NYP +RK L L  DDV+EQ P DI+YV+S YAPLS+RL Q L+ PGW++I++VL +LPGP  EE Q   + +++ + PGE+    TLV F+GG TFAEIAALR+LSQ ED  TEY++ATT ++NG++++++L+
Sbjct:    6 SYGRVNLNVLREAVRRELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKGSRLPA--ADVKNIIFLVRPRLELMDIIAENVLSEDRRGPTR-DFHILFVPRRSLLCEQRLKDLGVLGSFIHREEYSLDLIPFDGDLLSMESEGAFKECYLEGDQTSLYHAAKGLMTLQALYGTIPQIFGKGECARQVANMMVRMKREFTGSQNSVFPVFDNLLLLDRNVDLLTPLASQLTYEGLIDEIYGIQNSYVKLPPEKF-APKKQGGGSGGKDLPTEAKKLQLNSAEELYAEIRDKNFNAVGSVLSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARGSLANHTSIAELIKDVTTSEDFFDKLTVEQEFMSGIDTDKVNSYIEDCIAQKHPLIKVLRLVCLQSVCNSGLKQKVLDYYRREILQTYGYEHILTLNNLEKAGLLKAQTGGRNNYPTIRKTLRLWMDDVNEQNPTDISYVYSGYAPLSVRLAQLLSRPGWRSIEEVLRILPGPHFEERQPLPTGLQKKRQPGENR--VTLVFFLGGVTFAEIAALRFLSQLEDGGTEYVIATTKLMNGNSWIEALM 594          

BLAST of vacuolar protein sorting-associated protein 33a vs. SwissProt
Match: gi|48428693|sp|Q9Y1I2.1|VP33A_DROME (RecName: Full=Vacuolar protein sorting-associated protein 33A; AltName: Full=Protein carnation)

HSP 1 Score: 427.943 bits (1099), Expect = 8.116e-142
Identity = 251/622 (40.35%), Postives = 373/622 (59.97%), Query Frame = 0

Query:    9 IQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPG-RLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISK----IDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDS---------SDSEEPNPFG---MKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDK-IPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQT-SRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHP-GWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTT-------------LVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLLT 597
            +   LL++ A RELL  LD+  G+K++V D+ +  PLDL+ +     +R + +++ LKP   LP ++    NVV+V RP V LME +A +VK    +   R ++HI+F P+RS LC  +L+   V GSF  ++EL+  + PLD D++SME    FRD  ++ D + L Q A G++ LQ ++G IP++ GKG  A +V+E   Q+ +    DE  + +     +D LI++DR IDL+SP  TQLTYEGLIDE + I  N + LPAE F +  +            E  +  G    K I L+S ++LY+ELR+K+FN +   L++KAR +  QM      K+VQE++ FV+  +P++   +++ S HT IA L+ E  N  RF ++L  EQE +   + D+P  Y+ED      ++  VLRL+C+Q    SG K K+L  Y+R ++  YG + LLT+ NL++ GLL   LQT SR Y VLRK L+LT DD  E +P DI+YVHS YAPL+ R+V++   P GW+T+K  ++ LPGPT E+ Q     +      G  H  TT             LV FVGGCTFAEIAALR+L+ +ED+  E+++ATT +VN  +F+ SL++
Sbjct:   12 VNLQLLQEAACRELLQQLDRIEGSKVIVLDETMIGPLDLVTRPKLFADRGI-RLLALKPELHLPREVA---NVVYVMRPRVALMEQLAAHVKAGGRAAAGR-QYHILFAPRRSCLCVSQLEVSGVLGSFGNIEELAWNYLPLDVDLVSMEMPNAFRDVSVDGDTSSLYQAAVGLVQLQRLYGRIPKIYGKGEFAHRVWEHAKQLGR----DERTLYNGDKGVVDQLILLDRSIDLLSPLATQLTYEGLIDEFYGIRQNKLTLPAENFPSDGALPGGGGSGPRVEESQSLLGDTEKKTILLHSGEQLYAELRNKHFNEVTKLLARKAREIHVQMHATSQDKSVQEIKSFVENLLPQLMAQKKATSEHTAIAGLLHEQVNAVRFADDLAAEQEFMVCADIDKPSAYIEDLIACRVELNRVLRLICMQCHAASGFKEKLLNHYKRELVHVYGLEVLLTISNLEKSGLL--HLQTESRAYSVLRKTLHLTVDDNVEIEPKDISYVHSFYAPLTARIVEHSLKPLGWQTLKSQINNLPGPTFEDFQAQLVGI------GGRHTVTTVSEGSLLNVPRVVLVCFVGGCTFAEIAALRFLAAQEDNNVEFLIATTKVVNKHSFLDSLMS 616          

BLAST of vacuolar protein sorting-associated protein 33a vs. SwissProt
Match: gi|28201911|sp|Q94KJ7.1|VPS33_ARATH (RecName: Full=Vacuolar protein-sorting-associated protein 33 homolog; Short=AtVPS33)

HSP 1 Score: 336.265 bits (861), Expect = 1.495e-106
Identity = 207/592 (34.97%), Postives = 343/592 (57.94%), Query Frame = 0

Query:   14 LRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVE-KMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVIS-KIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQK---TSSNVKRIKPP----GEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            +RD + REL++ L    GTK LV D  L+  + LI   S LKE  +E + +  +P +      +   VV++ R ++  M+ +A +++  + +   + ++++ FVP+RS+ CE  L++++V+   T + E  L+  PLD DVIS E     +D  ++ D + L  +AK +  L+  FG+I ++  KG A+ +V + + +M+ E  V+   V   ++DTLI++DR++D+++P  +QLTYEGLIDE+  I+   V++ +    A             MKV  LNS D+L+ E RD NF  +   L +KA  +     E +  +TV EL+ FV K+  +      ++ H  +A+ +   T+++ F   L +EQ L+  +N D   EY+E+     E + +VLRL+ L +V NSG+  K  +  R  +L +YG++H++TL NL++ GLL  Q +   N+  +++ L L  +D D  +PNDIAYV+S YAPLSIRL+Q   H GW+ ++D+L LLPGP LE  +    +S +V  +        +  +S  LV+F+GG TFAEI+ALRYL+ +E    + +VATT IVNG T +++ +
Sbjct:   17 IRDKSERELVNLLKDVRGTKCLVIDPKLSGSVSLIIPTSKLKELGLELRHLTAEPVQ-----TECTKVVYLVRSQLSFMKFIASHIQ-NDIAKAIQRDYYVYFVPRRSVACEKILEQEKVHNLVT-VKEFPLYMVPLDEDVISFELELSEKDCLVDGDVSSLWHIAKAIHELEFSFGVISKMRAKGKASVRVADILNRMQVEEPVNSNDVGRPEVDTLILLDREVDMVTPMCSQLTYEGLIDEILHISNGAVEVDSSVMGAQQEGKK-------MKV-PLNSSDKLFKETRDLNFEVVVQVLRQKAMTMKEDYTEINSTQTVSELKDFVKKLNSLP----EMTRHIHLAQHLTTFTSKQSFNSQLDMEQTLVEAENYDICYEYIEEMIHKQEPLTNVLRLLVLFSVTNSGLPKKQFDYIRMELLHSYGFEHVVTLNNLEKAGLLKKQ-EFKSNWLTVKRTLKLIVEDTDTSRPNDIAYVYSGYAPLSIRLIQQAIHSGWRPMEDILKLLPGPHLETKRSGFPSSPSVDSLHGASNGVADGRRSIVLVVFIGGVTFAEISALRYLASKEGMAYDLIVATTKIVNGATLIETFM 588          

BLAST of vacuolar protein sorting-associated protein 33a vs. SwissProt
Match: gi|82178461|sp|Q58EN8.1|VP33B_DANRE (RecName: Full=Vacuolar protein sorting-associated protein 33B)

HSP 1 Score: 275.404 bits (703), Expect = 5.882e-83
Identity = 193/625 (30.88%), Postives = 326/625 (52.16%), Query Frame = 0

Query:   11 FDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKM--VPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFV-DKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQ-------------------------------LQTSRNYPVLRKRLNL---TRDDVDEQQPNDIAYVHS-VYAPLSIRLV-QNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            F LL+ LAR +L+  L++  G K L  D  L +PLD IA  + LK+ +V+K+  V LKP      +   + + F+ RP +  ++ ++D V   +   G    + I+F P++   CE  L+E+ VYG  T  DE + +  PLD D++S+E  + FRD  L  D   +      +  LQ+V+G   +V G G  A+ V+E   ++ +E      Q   +   + +IDR +D ++P  +Q+ YEGL+D++F I  ++V+        PD + S++     +KV+ LNSQD++++E+R+++F+ +   LS+KA+ +    D+R     +Q+++ FV D++  +K   + LS+H   +E + +   ++ F E LK E  L+        + Y+E+       ++  LRL+CL ++  +G+  K   S +   LQ+YG +HLLT  NL +LGLL  Q                               L    N+  L KRL L   + ++ D + P D+AY+ S  Y PLS +L+ Q L   GW  +++V  +L G        +SS+  R K  G   Q   LV+F+GGCT++EI+ALR+L +  +    ++V TTAI N    +++LL
Sbjct:   15 FSLLKRLARDQLIFLLEQLPGKKDLFIDADLMSPLDRIANVTILKQHEVDKLYKVELKP-----IVSSSDQLCFLIRPRIQTVKWISDLVN-SDKVAGRFRRYKIIFTPQKFYACETVLEEQGVYGDVT-TDEWNFYILPLDDDILSLELPEFFRDNFLEGDQRWVTTGGGALHLLQSVYGSFSKVYGIGRCAKMVYESWRELMEEGEQRTRQ--PEFAKVFLIDRDVDFVTPLCSQVVYEGLVDDIFRIKCSSVEF------GPDVTSSDKS----IKVM-LNSQDKVFNEIRNEHFSNVFGFLSQKAKNLQTAYDKRR-GMDIQQMKAFVADELKGLKQEHRLLSLHIGASESIMKKKTKQDFQELLKTEHSLLEGFEIRECIAYIEEHINRQVSMIDSLRLLCLLSITENGLLSKDYRSLKAQYLQSYGIEHLLTFANLRQLGLLEEQQTGETLTVMESKVGKLVNDKTAGKLTDAFSSLAKKSNFRALSKRLALVPKSGEEYDLRVPRDMAYIFSGAYIPLSCKLIEQVLERDGWTGLEEVTRMLNGQDFAVTGGSSSSEARNKSNG---QRIILVMFLGGCTYSEISALRFLGK--ERGCRFIVLTTAITNSGRLLEALL 613          

BLAST of vacuolar protein sorting-associated protein 33a vs. SwissProt
Match: gi|109896157|sp|Q2HJ18.1|VP33B_BOVIN (RecName: Full=Vacuolar protein sorting-associated protein 33B)

HSP 1 Score: 262.692 bits (670), Expect = 3.700e-78
Identity = 186/626 (29.71%), Postives = 319/626 (50.96%), Query Frame = 0

Query:   11 FDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKM--VPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQS---LSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQ-------------------------------LQTSRNYPVLRKRLNL---TRDDVDEQQPNDIAYVHS-VYAPLSIRLV-QNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSL 595
            F +L+ LAR +L+  L++  G K L  +  L +PLD IA  S LK+ +V+K+  V  KP      L   E + F+ RP +  M  +A  V  ++ +  TR ++ ++F P++   CEM L+E+ VYG  +  DE +    PLD D++SME  + FRD+ L  D   +N +A+ +  L  ++G  P   G G  A+  +E   ++ +E   +      +I  + ++DR +D ++   +Q+ YEGL+D+ F I   +V         P+ + S++     +KV+ LN++D++++E+R+++F+ +   LS+KAR + AQ D R     +++++ FV +  ++K L+Q    LS+H    E + +   ++ F E +K E  L+   N      Y+E+        +  LRL+CL ++  +G+ PK   S +   LQ+YG +HLLT  NL R GLL+ Q                               L    N+  + K+LNL      + D + P D+AYV S  Y PLS R++ Q L   GW+ + +V+ LL    L     T  +         +     LV+F+GGCTF+EI+ALR+L +  +    ++  TTA+ N    ++++
Sbjct:   15 FSMLKRLARDQLIYLLEQLPGKKDLFIEADLMSPLDRIANVSILKQHEVDKLYKVENKPA-----LSSSEQLCFLVRPRIKNMRYIASLVNADKMAGRTR-KYKVIFSPQKFYACEMVLEEEGVYGDVS-CDEWAFSLLPLDVDLLSMELPEFFRDYFLEGDQRWINTLAQALHLLSTLYGPFPNCYGIGRCAKMSYELWKRLEEEEDGETKGRRPEIGHIFLLDRDVDFVTALCSQVVYEGLVDDTFRIKCGSVDF------GPEVTSSDK----SLKVL-LNAEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYDRRR-GMDIKQMKNFVSQ--ELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIRESTNYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFFNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFSGAYVPLSCRIIEQVLERRGWQGLDEVVRLLNCSELAFTDMTKDD-----KASSESLRLILVVFLGGCTFSEISALRFLGR--EKGYRFIFLTTAVTNSARLMEAM 612          

BLAST of vacuolar protein sorting-associated protein 33a vs. SwissProt
Match: gi|31077064|sp|Q9P7V6.1|VPS33_SCHPO (RecName: Full=Vacuolar protein sorting-associated protein 33)

HSP 1 Score: 259.61 bits (662), Expect = 2.486e-77
Identity = 185/608 (30.43%), Postives = 321/608 (52.80%), Query Frame = 0

Query:   14 LRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDP---TCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQM-RKELVVD----EPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLL------SPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDL--LPGP-----TLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLLTHVD 600
            +++ A  +LLD +D   G K L+ +  L+  L  I   +TL+E  + ++       +P D+ +    +++ RP     + VA +V+ +      R E  ++ +P  ++L E  L+E+ V+G    + E  L   PLD D++S+E         L  +    + L +    ++  +   G  PRV G+G  A K+ E + +  ++E  ++    E ++ +  D+++++DR +D I+PFLTQLTY G +DE+  I    VKLP+   +  ++S++     F +      S  ++  E+RD NFN IGP LSK AR +S+  + R  AKTV ++R FV K+  ++    SL+IHT +AE + + T    F + L+++  L++  ++      L++    +  +  V R++CL ++  +G++ K ++ YRR I QTYGY HLLT Q L   GLL      +  LQ S +Y        L +D+VDEQ P DIAY +S Y PLS+ +  ++   G    + +L L  +PG      TL++ +    N+K    PG+      LV F+GGCT+ E+AA R L ++ED   E+   TT +V G + +++ + +++
Sbjct:    5 VKEKATFKLLDLIDSVTGKKSLLLERDLSGILGQIVTTNTLQEHGIPQVYWFNEN-IPNDIEK--KTIYLCRPTYENAKLVATHVR-QFQRDMLRIESTVIVLPTSNILFETVLQEEGVFGEL-LVTEWPLHAVPLDKDLLSLE---------LGPEKLEESLLQRSTDALIDFERTHGRFPRVSGRGPYAAKMLELLEKTYQEEATINFGKVEGEISALYDSVLLVDRSLDRITPFLTQLTYFGFLDEILGIQQMNVKLPSSLVNRNEASNTGPMKKFSLSS----SSSQITKEIRDINFNCIGPYLSKIARKLSSDFEGRRQAKTVNQIRDFVSKLGSLQSEHTSLNIHTGLAETLVQHTKNNYFQKLLQLQHLLVSHADSFTQFNLLDEIIYAEAPVEEVFRVLCLASITTNGLRRKDIDHYRREITQTYGYYHLLTFQALIDAGLLRLRQSTNISLQKSLSYSTWLNTYPLVKDEVDEQNPEDIAYTYSGYGPLSVHIAYDILK-GRDNEEKILKLQNMPGTYVDKWTLDQEKVMPKNLK-TNVPGKRR---VLVFFIGGCTYTELAAFRLLQEKEDL-YEFTFMTTGMVTGSSLIRAFIPNIE 588          

BLAST of vacuolar protein sorting-associated protein 33a vs. SwissProt
Match: gi|313104046|sp|Q9H267.2|VP33B_HUMAN (RecName: Full=Vacuolar protein sorting-associated protein 33B; Short=hVPS33B)

HSP 1 Score: 258.455 bits (659), Expect = 1.197e-76
Identity = 186/631 (29.48%), Postives = 319/631 (50.55%), Query Frame = 0

Query:   11 FDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKM--VPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQS---LSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQ-------------------------------LQTSRNYPVLRKRLNL---TRDDVDEQQPNDIAYVH-SVYAPLSIRLV-QNLAHPGWKTIKDVLDLLPGP-----TLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSL 595
            F +L+ LAR +L+  L++  G K L  +  L +PLD IA  S LK+ +V+K+  V  KP      L   E + F+ RP +  M  +A  V  ++ +  TR ++ ++F P++   CEM L+E+ +YG  +  DE +    PLD D++SME  + FRD+ L  D   +N VA+ +  L  ++G  P   G G  A+  +E    + +E   +      +I  + ++DR +D ++   +Q+ YEGL+D+ F I   +V         P+ + S++     +KV+ LN++D++++E+R+++F+ +   LS+KAR + AQ D R     +++++ FV +  ++K L+Q    LS+H    E + +   ++ F E +K E  L+   N      Y+E+        +  LRL+CL ++  +G+ PK   S +   LQ+YG +HLLT  NL R GLL+ Q                               L    N+  + K+LNL      + D + P D+AYV    Y PLS R++ Q L    W+ + +V+ LL         + +  K SS   R+           LV+F+GGCTF+EI+ALR+L +  +    ++  TTA+ N    ++++
Sbjct:   15 FSMLKRLARDQLIYLLEQLPGKKDLFIEADLMSPLDRIANVSILKQHEVDKLYKVENKPA-----LSSNEQLCFLVRPRIKNMRYIASLVNADKLAGRTR-KYKVIFSPQKFYACEMVLEEEGIYGDVS-CDEWAFSLLPLDVDLLSMELPEFFRDYFLEGDQRWINTVAQALHLLSTLYGPFPNCYGIGRCAKMAYELWRNLEEEEDGETKGRRPEIGHIFLLDRDVDFVTALCSQVVYEGLVDDTFRIKCGSVDF------GPEVTSSDK----SLKVL-LNAEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYDRRR-GMDIKQMKNFVSQ--ELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFGGAYVPLSCRIIEQVLERRSWQGLDEVVRLLNCSDFAFTDMTKEDKASSESLRL----------ILVVFLGGCTFSEISALRFLGR--EKGYRFIFLTTAVTNSARLMEAM 612          

BLAST of vacuolar protein sorting-associated protein 33a vs. SwissProt
Match: gi|23396902|sp|P59016.1|VP33B_MOUSE (RecName: Full=Vacuolar protein sorting-associated protein 33B)

HSP 1 Score: 256.529 bits (654), Expect = 6.218e-76
Identity = 186/632 (29.43%), Postives = 322/632 (50.95%), Query Frame = 0

Query:   11 FDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKM--VPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQS---LSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQ-------------------------------LQTSRNYPVLRKRLNL---TRDDVDEQQPNDIAYVHS-VYAPLSIRLV-QNLAHPGWKTIKDVLDLLPGP------TLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSL 595
            F +L+ LAR +L+  L++  G K L  +  L +PLD IA  S LK+ +V+K+  V  KP      L   E + F+ RP +  M  +A  V  ++ +   R ++ ++  P++   CEM L+E+ VYG  +  DE +    PLD D++SME  + FRD+ L  D   +N VA+ +  L  ++G  P   G G  A+  ++   ++ +E   +      +I  + ++DR +D ++   +Q+ YEGL+D+ F I   +V         P+ + S++     +KV+ LN++D+++SE+R+++F+ +   LS+KAR + AQ D R     +++++ FV +  ++K L+Q    LS+H    E + +   ++ F E +K E  L+   N      Y+E+        +  LRL+CL ++  +G+ PK   S +   LQ+YG +HLLT  NL R GLL+ Q                               L    N+  + K+LNL      + D + P D+AYV S  Y PLS R++ Q L    W+ + +V+ LL         T +E + +S +++ I           LV+F+GGCTF+EI+ALR+L +  +    ++  TTA+ N    ++++
Sbjct:   15 FSMLKRLARDQLIYLLEQLPGKKDLFIEADLMSPLDRIANVSILKQHEVDKLYKVENKPA-----LSANEQLCFLVRPRIKNMRYIASLVNADKLAGRIR-KYKVILSPQKFYACEMVLEEEGVYGDVS-CDEWAFSLLPLDVDLLSMELPEFFRDYFLEGDQRWINTVAQALHLLSTLYGPFPNCYGIGRCAKMSYDLWRKLEEEEDSETKGRKPEIGHIFLLDRDVDFVTALCSQVVYEGLVDDTFRIKCGSVDF------GPEVTSSDK----SLKVL-LNAEDKVFSEIRNEHFSNVFGFLSQKARNLQAQYDRRR-GMDIKQMKNFVSQ--ELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFSGAYVPLSCRIIEQVLDRRSWQGLDEVVRLLNCSEFAFTDTAKEDKASSESLRLI-----------LVVFLGGCTFSEISALRFLGR--EKGYRFIFLTTAVTNSARLMEAM 612          

BLAST of vacuolar protein sorting-associated protein 33a vs. nr
Match: gi|1126158618|ref|XP_019615680.1| (PREDICTED: vacuolar protein sorting-associated protein 33A-like [Branchiostoma belcheri])

HSP 1 Score: 621.698 bits (1602), Expect = 0.000e+0
Identity = 308/608 (50.66%), Postives = 426/608 (70.07%), Query Frame = 0

Query:    2 SFRSDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKF-HAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPP---GEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLLTHVDDNLAP 605
            S  S   +   +LR+ AR+ELL+ LDK  GTK +VWD+ LT P  LIA++S LKE +V+KM  L+PGRLP       N++F+ RP++ LME VA N+  EE + G+R EFHI FVP++SLLCE +LKE  VYG+F+ +DE +L   P D+D++SME    FRD +L DD T +   AK ++++QA++GIIP + GKG  AR V + M +MR+EL   EPQ+  +ID L+++DR +DL++P  TQLTYEGLIDE+F IN  +VKLP EKF  A   +   +  P   K + LNS +ELY+E RD+NF+AIGP LS+KA+ +SAQ +ERH AKTV E++ FV ++P M+  + SL+ HTTIAE++KE T+   F+E+L V+QE +N  + D+   Y+ED     E I+ +LRLVC+Q+V N+G+K K L+ Y+R ILQTYGY+HL+TL NL+R GLL+  LQ  R YP  RK L L  DDV EQ PNDI+YV S YAPLS+RL Q LA PGW++I++VL +LPGPT EEAQ+    +++ +     G +    TLV F+GG T+AEIAALR+LSQ+EDS ++Y++ATT I+NG T+++ L+    D L P
Sbjct:    4 SHLSGGRVNLAILREAARKELLECLDKCDGTKAVVWDEQLTGPFGLIAEYSLLKEHEVDKMFQLRPGRLPA--ANVNNIIFLCRPKLALMEIVAQNILKEEETGGSRKEFHIFFVPRKSLLCEQKLKELGVYGTFSNVDEYTLDLIPFDSDLLSMEIDSTFRDCYLEDDMTSMYYAAKSLMSVQALYGIIPTICGKGDCARHVADMMLRMRRELAGTEPQITPQIDCLLLLDRTVDLMTPLPTQLTYEGLIDEIFGINNTSVKLPPEKFASAKQQTGGPQEMPTEPKKLVLNSAEELYAEARDRNFHAIGPLLSRKAKLISAQFEERHGAKTVGEIKQFVSRLPHMQAAKISLANHTTIAEMIKEVTDSDHFMESLTVQQEFLNGIDTDKVNPYIEDCIARKEPIIKILRLVCIQSVANNGLKQKALDYYKREILQTYGYEHLITLNNLERAGLLT--LQKQRTYPTTRKTLRLMVDDVSEQNPNDISYVFSGYAPLSVRLAQFLARPGWRSIEEVLRILPGPTFEEAQRIPVGLRKKRTSVGGGSESPKVTLVYFLGGVTYAEIAALRFLSQQEDSASDYIIATTKIINGHTWLEGLM----DTLCP 603          

BLAST of vacuolar protein sorting-associated protein 33a vs. nr
Match: gi|1207915106|ref|XP_021346293.1| (vacuolar protein sorting-associated protein 33A-like isoform X2 [Mizuhopecten yessoensis] >gi|1205907277|gb|OWF53882.1| Small G protein signaling modulator 1 [Mizuhopecten yessoensis])

HSP 1 Score: 613.994 bits (1582), Expect = 0.000e+0
Identity = 302/601 (50.25%), Postives = 426/601 (70.88%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGM----KVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNV-KRIKPPGEDHQST-----TLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSL 595
            S   I   LLRD  RRELL+ LD+ +GTK LVWDD LT    LIA++S LKE +VEKM PL+   LP      +N++FVTRP++ LME +A N+  EE   G R ++HI+FVP+RSLLC+ +LKE  VYG+ T  +E  L   P D+D++SME    FR+ +L +D T ++  A+ ++ LQA++GIIP + GKG  A+ + + M +MR+EL   EPQ+  +ID L+I+DR +DL++P L+QLTYEGLIDEMF IN  +VKLP EKF    + + ++ +P G+    K + LNS DELY++LRDKNFNA+G  +SKKA+ ++A+ DERH AKTV E++ FV K+P ++ +R SLS HT+IAELVKEAT+   F+++L+ +QE IN  + D+   Y+ED     E I  VLRLVC+Q+  N+G+KPKVL+ Y+R I+QTYGY+H++TL N+++ GLL P    +R+YP +RK + L  DDV+EQ P+DIAYV+S +APLS RLVQ  A PGW++I +VL+LLPGPT+EE Q+    + KR    G  H ST     TLV F+GG T+AEIAALR+L+Q++D  T+Y++ATT +++G +F+KS+
Sbjct:    8 STGKINLSLLRDFYRRELLESLDRCSGTKALVWDDALTGAFGLIAEYSLLKEHEVEKMFPLRSSGLPPS--NVQNIIFVTRPKLSLMEQIAQNLLQEEQKGGFRKDYHIIFVPRRSLLCDKKLKEMGVYGTITNFEEFQLSLIPFDSDLLSMEMESSFRECYLENDFTSMHYAAQSLMTLQALYGIIPNICGKGDCAKHIVDMMLRMRRELGGREPQITPQIDNLLILDRNVDLLTPLLSQLTYEGLIDEMFGINNTSVKLPPEKFQ---TGNQQQEDPQGVSSEPKKVILNSLDELYADLRDKNFNAVGSIVSKKAKVITAEFDERHTAKTVSEMKQFVSKLPHLQQVRASLSTHTSIAELVKEATDTDEFMDSLRCQQEFINGVDTDKIHPYIEDCMGRQEPINKVLRLVCIQSFCNNGLKPKVLDHYKREIIQTYGYEHMVTLMNIEKAGLLRP--HGNRSYPAIRKSMRLIVDDVNEQNPHDIAYVYSGFAPLSARLVQYYARPGWRSITEVLNLLPGPTVEEIQQIPVALRKRRSSIGSTHSSTGEQKVTLVFFLGGVTYAEIAALRFLAQQDDGGTDYLIATTKVISGTSFLKSI 601          

BLAST of vacuolar protein sorting-associated protein 33a vs. nr
Match: gi|1308466568|ref|XP_023143869.1| (vacuolar protein sorting-associated protein 33A [Amphiprion ocellaris])

HSP 1 Score: 613.224 bits (1580), Expect = 0.000e+0
Identity = 297/592 (50.17%), Postives = 415/592 (70.10%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            S   +  ++LR+ AR+EL + LDK AG+K +VWD+ LT P  LIA++S LKE +VEKM  LK GRLP      +N++F  RP + LM+ +A+NV F E  H +  +FHI+FVP+RS+LCE RLKE+ V GSF  +DE  L   P D D++SME    FR+ +L +D T L   AKG++ LQA++G IP++ GKG  AR V   M +M++E    + Q++   DTL+++DR +DL++P  TQLTYEGLIDE++ IN   VKLP EKF      ++ +  P   K + LNS +ELY+E+RDKNFNA+G +LSKKA+ +SA  +ERH+AKTV E++ FV ++P M+  R SL+ HT+IAEL+K+ T  + F +NL VEQE +   + D+   Y+ED     + ++ +LRLVC+Q+V N+G+K KVL+ Y+R ILQTYGY+H+LTL NL++ GLL PQ  +  NYP +RK L L  +D +EQ PNDI+YV+S YAPLSIRL Q LA PGW++I++VL +LPGP  EE Q+  S + + +  GE+   TTLV F+GG T+AEIAALR+LSQ EDS  EY++ATT +VNG T++KSL+
Sbjct:    6 SYGRVNLNILREAARKELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKSGRLPS--ADVKNIIFFVRPRLELMDIIAENV-FSEDKHHSSRDFHILFVPRRSMLCEQRLKEQGVLGSFINIDEYILDLIPYDGDLLSMEYESAFRECYLENDQTSLYHTAKGLMTLQALYGTIPQIYGKGDCARHVANMMLRMKREFAGTQNQILPVFDTLLLLDRNVDLLTPLATQLTYEGLIDEIYGINNGYVKLPPEKFAQKKQGEASKDLPTEPKKLQLNSAEELYAEIRDKNFNAVGAALSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARSSLANHTSIAELIKDITTSEAFFDNLTVEQEFMTGVDTDKVNTYIEDCIAQKDPLIKILRLVCMQSVCNNGLKQKVLDYYKREILQTYGYEHILTLNNLEKGGLLKPQTSSRNNYPTIRKTLKLWMEDANEQNPNDISYVYSGYAPLSIRLTQVLARPGWRSIEEVLKMLPGPHFEERQQLPSGLHKKRQQGENR--TTLVFFLGGVTYAEIAALRFLSQMEDSGMEYIIATTKLVNGTTWIKSLM 592          

BLAST of vacuolar protein sorting-associated protein 33a vs. nr
Match: gi|1228990842|ref|XP_022059922.1| (vacuolar protein sorting-associated protein 33A [Acanthochromis polyacanthus])

HSP 1 Score: 613.224 bits (1580), Expect = 0.000e+0
Identity = 297/592 (50.17%), Postives = 415/592 (70.10%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            S   +  ++LR+ AR+EL + LDK AG+K +VWD+ LT P  LIA++S LKE +VEKM  LK GRLP      +N++F  RP + LM+ +A+NV F E  H +  +FHI+FVP+RS+LCE RLKE+ V GSF  +DE  L   P D D++SME    FR+ +L +D T L   AKG++ LQA++G IP++ GKG  AR V   M +M++E    + Q++   DTL+++DR +DL++P  TQLTYEGLIDE++ IN   VKLP EKF      ++ +  P   K + LNS +ELY+E+RDKNFNA+G +LSKKA+ +SA  +ERH+AKTV E++ FV ++P M+  R SL+ HT+IAEL+K+ T  + F +NL VEQE +   + D+   Y+ED     + ++ +LRLVC+Q+V N+G+K KVL+ Y+R ILQTYGY+H+LTL NL++ GLL PQ  +  NYP +RK L L  +D +EQ PNDI+YV+S YAPLSIRL Q LA PGW++I++VL +LPGP  EE Q+  S + + +  GE+   TTLV F+GG T+AEIAALR+LSQ EDS  EY++ATT +VNG T++KSL+
Sbjct:    6 SYGRVNLNILREAARKELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKSGRLPS--ADVKNIIFFVRPRLELMDIIAENV-FSEDKHHSSRDFHILFVPRRSMLCEQRLKEQGVLGSFINIDEYILDLIPYDGDLLSMEYESAFRECYLENDQTSLYHTAKGLMTLQALYGTIPQIYGKGDCARHVANMMLRMKREFAGTQNQILPVFDTLLLLDRNVDLLTPLATQLTYEGLIDEIYGINNGYVKLPPEKFAQKKQGEASKDLPTEPKKLQLNSAEELYAEIRDKNFNAVGAALSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARSSLANHTSIAELIKDITTSEAFFDNLTVEQEFMTGVDTDKVNTYIEDCIAQKDPLIKILRLVCMQSVCNNGLKQKVLDYYKREILQTYGYEHILTLNNLEKGGLLKPQTSSRNNYPTIRKTLKLWMEDANEQNPNDISYVYSGYAPLSIRLTQVLARPGWRSIEEVLKMLPGPHFEERQQLPSGLHKKRQQGENR--TTLVFFLGGVTYAEIAALRFLSQMEDSGMEYIIATTKLVNGTTWIKSLM 592          

BLAST of vacuolar protein sorting-associated protein 33a vs. nr
Match: gi|1242808108|ref|XP_022344839.1| (vacuolar protein sorting-associated protein 33A-like isoform X1 [Crassostrea virginica])

HSP 1 Score: 612.068 bits (1577), Expect = 0.000e+0
Identity = 303/598 (50.67%), Postives = 426/598 (71.24%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDH------QSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            S+  +  +LLRD  RRELL+ LDK  G+K LVWDD LT P  LIA++S LKE +VEKM PL    LP      +N++FVTRP++HLME ++ N+  EEH  G R E+HI+FVP++SLLCE +LKE  VYG+   ++E +L   P D+D++SME    F++ +L +D T +  VA+ ++ LQ ++GIIP + GKG  A+ V + M +MR+EL   EPQ+  +ID L+I+DR +DL++P L+QLTY+GLIDE+F IN  +VKLP EKF +  SS + E  P   K I LNS DELY ELRDKNFNA+G  +SKKA+ ++A+ DERH AKTV E++ FV K+P ++ +RQSLS+HT+IAEL+KE T+ + FL++L+ +QE IN  + D+   Y+ED     E +  VLRL+C+Q+  N+G+K KVL+ Y+R I+QTYGY+HL+TL NL++ GLL P  Q  RNYP +RK L L  D+V+EQ PNDI+YV+S YAPLSIRL Q  A PGW++I +VL+LLPGPT+EE Q+    +++ +            Q  TLV+F+GG TFAEIAALR+L+Q++D  T+Y++ATT I++G T +++L+
Sbjct:    8 SNGKLNLNLLRDYYRRELLECLDKCLGSKALVWDDALTGPFGLIAEYSLLKEHEVEKMFPLSDKGLPPS--NVQNIIFVTRPKLHLMEQISQNLLQEEHKGGFRKEYHIIFVPRKSLLCEKKLKESGVYGTLHNIEEFNLSLIPFDSDLMSMEMEGAFKECYLENDYTSMFYVAQSLMTLQFLYGIIPNIQGKGDCAKNVVDMMLRMRRELGGREPQITPQIDNLLILDRNVDLLTPLLSQLTYDGLIDEIFGINNTSVKLPPEKFVS--SSQAPEEAPTEKKKIILNSSDELYGELRDKNFNAVGSLVSKKAKTITAEFDERHAAKTVSEMKHFVSKLPHLQQMRQSLSVHTSIAELIKEHTDSEEFLDSLRCQQEFINGVDTDKVNTYVEDCIGKMEPLDKVLRLICIQSYCNNGLKTKVLDYYKREIIQTYGYEHLVTLMNLEKAGLLRPSGQ--RNYPTIRKSLRLIVDEVNEQNPNDISYVYSGYAPLSIRLAQYHARPGWRSITEVLNLLPGPTIEEIQQIPVALRKRRSSITSSHSSVMDQKVTLVVFLGGVTFAEIAALRFLAQQDDGGTDYVIATTKIISGSTLLETLM 599          

BLAST of vacuolar protein sorting-associated protein 33a vs. nr
Match: gi|1168973140|ref|XP_020446370.1| (vacuolar protein sorting-associated protein 33A [Monopterus albus])

HSP 1 Score: 612.068 bits (1577), Expect = 0.000e+0
Identity = 295/592 (49.83%), Postives = 419/592 (70.78%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            S   +  ++LR+ AR+EL + LDK AG+K +VWD+ LT P  LIA++S LKE +VEKM  LK GRLP    + +N++F  RP + LM+ +A+NV  E+  H +R +FHI+FVP+RSLLCE RLKE+ V GSF  +DE  L   P D D++SME    FR+ +L +D T L   AKG++ LQA++G IP++ GKG  AR V   M +M++E    + Q++   DTL+++DR +DL++P  TQLTYEGLIDE++ I+   VKLP EKF      ++ +  P   K + LNS +ELY+E+RDKNFNA+G +LSKKA+ +SA  +ERH+AKTV E++ FV ++P M+  R SL+ HT+IAEL+K+ T  + F +NL VEQE +   + D+   Y+ED+    + ++ +LRLVC+Q+V N+G+K KVL+ Y+R ILQTYGY+H+LTL NL+++GLL PQ  +  NYP +RK L L  +D +EQ PNDI+YV+S YAPLSIRL Q LA PGW++I++VL +LPGP  EE Q+  + + + +  GE+   TTLV F+GG T+AEIAALR+LSQ EDS  EY++ATT ++NG T++KSL+
Sbjct:    6 SYGRVNLNILREAARKELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKSGRLPS--AEVKNIIFFVRPRLELMDIIAENVFREDKLHSSR-DFHILFVPRRSLLCEQRLKEQGVLGSFINIDEYILDLIPYDGDLLSMEYESAFRECYLENDQTSLYHTAKGLMTLQALYGTIPQIYGKGECARHVANMMLRMKREFAGSQNQILPVFDTLLLLDRNVDLLTPLATQLTYEGLIDEIYGISNGYVKLPPEKFAQKKQGEASKDLPTEPKKLQLNSAEELYAEIRDKNFNAVGAALSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARSSLANHTSIAELIKDITTSEAFFDNLTVEQEFMTGVDTDKVNIYIEDSIAQKDPLIKILRLVCMQSVCNNGLKQKVLDYYKREILQTYGYEHILTLNNLEKVGLLKPQTSSRNNYPTIRKTLKLWMEDANEQNPNDISYVYSGYAPLSIRLTQVLARPGWRSIEEVLKMLPGPHFEERQQLPAGLHKKRQQGENR--TTLVFFLGGVTYAEIAALRFLSQMEDSGMEYIIATTKLINGTTWIKSLM 592          

BLAST of vacuolar protein sorting-associated protein 33a vs. nr
Match: gi|657548274|ref|XP_008279062.1| (PREDICTED: vacuolar protein sorting-associated protein 33A [Stegastes partitus])

HSP 1 Score: 610.912 bits (1574), Expect = 0.000e+0
Identity = 295/592 (49.83%), Postives = 416/592 (70.27%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            S   +  ++LR+ AR+EL + LDK AG+K +VWD+ LT P  LIA++S LKE +VEKM  LK GRLP      +N++F  RP + LM+ +A+NV  E+  H +R +FHI+FVP+RS+LCE RLKE+ V GSF  +DE  L   P D D++SME    FR+ +L +D T L   AKG++ LQA++G IP++ GKG  AR V   M +M++E    + Q++   DTL+++DR +DL++P  TQLTYEGLIDE++ IN   VKLP EKF      ++ +  P   K + LNS +ELY+E+RDKNFNA+G +LSKKA+ +SA  +ERH+AKTV E++ FV ++P M+  R SL+ HT+IAEL+K+ T  + F +NL VEQE +   + D+   Y+ED+    + ++ +LRLVC+Q+V N+G+K KV + Y+R ILQTYGY+H+LTL NL++ GLL PQ  +  NYP +RK L L  +D +EQ PNDI+YV+S YAPLSIRL Q LA PGW++I++VL +LPGP  EE Q+  S + + +  GE+   TTLV F+GG T+AEIAALR+LSQ EDS  EY++ATT ++NG T++KSL+
Sbjct:    6 SYGRVNLNILREAARKELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKSGRLPS--ADVKNIIFFVRPRLELMDIIAENVFSEDKLHSSR-DFHILFVPRRSMLCEQRLKEQGVLGSFVNIDEYILDLIPYDGDLLSMEYESAFRECYLENDQTSLYHTAKGLMTLQALYGTIPQIYGKGDCARHVANMMLRMKREFAGTQNQILPVFDTLLLLDRNVDLLTPLATQLTYEGLIDEIYGINNGYVKLPPEKFAQKKQGEASKDLPTEPKKLQLNSAEELYAEIRDKNFNAVGAALSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARSSLANHTSIAELIKDITTSEAFFDNLTVEQEFMTGVDTDKVNTYIEDSIAQKDPLIKILRLVCMQSVCNNGLKQKVFDYYKREILQTYGYEHILTLNNLEKGGLLKPQTSSRNNYPTIRKTLKLWMEDANEQNPNDISYVYSGYAPLSIRLTQVLARPGWRSIEEVLKMLPGPHFEERQQLPSGLHKKRQQGENR--TTLVFFLGGVTYAEIAALRFLSQMEDSGMEYIIATTKLINGTTWIKSLM 592          

BLAST of vacuolar protein sorting-associated protein 33a vs. nr
Match: gi|443686017|gb|ELT89435.1| (hypothetical protein CAPTEDRAFT_101629 [Capitella teleta])

HSP 1 Score: 610.912 bits (1574), Expect = 0.000e+0
Identity = 305/603 (50.58%), Postives = 420/603 (69.65%), Query Frame = 0

Query:    2 SFRSDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRL-PVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKF--------HAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSL 595
            S  S+  +   +LR+  RRELL+ LDK  G+K LVWDD LT P+ LIA++S LKE +V+KM PL+PGRL P ++   +N++F+ RP + LME +A NV  EE   G R +FH+ FVP++S LCE  LK+  VYG+FT +DE  L   P D DV+SME    +RD  LNDD T L   AK ++ LQA++GIIP + GKG  A+ V E M +MR+E+   EPQ++++ID L+IIDR  D ++P L+QLTYEGLIDE+F IN  TVKLP EKF         A ++  + EP  F      LNS +EL++ELRD+NFNA+GP LS+KA+ ++AQ DERH AKTV E++ FV K+P M+  + SL+ HT+IAELVKE T ++ F+ +L+ +QE       D+   Y+ED     E +V VLRL+C+Q + N+G KPK+LE Y+R ILQTYG++HL+TL NL++ GLL  ++Q +R YP LRK L L  ++V+EQ PNDI+YV+S YAPLS+RL Q    PGW++I + L+LLPGPT++E Q+    ++R    GE  Q  TLV F+GG T AEIAALR+LS +EDSP+EY+VATT ++ GD+++KS+
Sbjct:    3 SHLSNGPVNLGVLRESYRRELLECLDKCTGSKALVWDDNLTGPIGLIAEYSLLKEHEVDKMFPLRPGRLQPSNV---QNIIFICRPNLALMENIAQNVLKEEEMGGFRKDFHLFFVPRKSFLCEKALKDLGVYGTFTNVDEFCLDLIPFDGDVLSMEMDLSYRDCVLNDDWTSLYHAAKALMTLQALYGIIPNIQGKGPCAKHVVEMMLRMRREMAGQEPQIMAQIDHLLIIDRSTDPLTPLLSQLTYEGLIDEIFGINNTTVKLPGEKFAPQGKGGDEAAENITTTEPKKF-----ILNSTEELFAELRDRNFNAVGPLLSRKAKLIAAQFDERHSAKTVGEIKQFVSKLPHMQAAKTSLATHTSIAELVKEETTKEEFMASLRAQQEFYGGMETDKAHPYIEDCIAKLEPLVKVLRLICIQCICNNGFKPKMLEFYKREILQTYGFEHLITLHNLEKAGLL--KIQGARTYPTLRKTLKLIVEEVNEQNPNDISYVYSGYAPLSVRLAQIAHRPGWRSIHEALNLLPGPTIDEIQQIPMELRRRSQTGE--QKVTLVFFLGGVTHAEIAALRFLSHQEDSPSEYIVATTKLITGDSWLKSI 593          

BLAST of vacuolar protein sorting-associated protein 33a vs. nr
Match: gi|348516222|ref|XP_003445638.1| (PREDICTED: vacuolar protein sorting-associated protein 33A isoform X1 [Oreochromis niloticus])

HSP 1 Score: 610.527 bits (1573), Expect = 0.000e+0
Identity = 295/592 (49.83%), Postives = 415/592 (70.10%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            S   +  ++LR+ AR+EL + LDK AG+K +VWD+ LT P  LIA++S LKE +VEKM  LK GRLP      +N++F  RP + LM+ +ADN+  E+  H +R +FHI+FVP+RS+LCE RLKE+ V GSF  +DE  L   P D D++SME    FR+ +L +D T L   AKG++ LQA++G IP++ GKG  AR V   M +M++E    + Q++   DTL+++DR +DL++P  TQLTYEGLIDE++ IN   VKLP EKF      +  +  P   K + LNS +ELY+E+RDKNFNA+G +LSKKA+ +SA  +ERH+AKTV E++ FV ++P M+  R SL+ HT+IAEL+K+ T  + F +NL VEQE +   + D+   Y+ED     + ++ +LRLVC+Q+V N+G+K KV++ Y+R ILQTYGY+H+LTL NL++ GLL PQ  +  NYP +RK L L  +D +EQ PNDI+YV+S Y+PLSIRL Q LA PGW++I++VL +LPGP  EE Q+  S + + +  GE+   TTLV F+GG T+AEIAALR+LSQ EDS  EY++ATT +VNG T++KSL+
Sbjct:    6 SYGRVNLNILREAARKELREFLDKCAGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKHGRLPP--ADVKNIIFFVRPRLELMDIIADNITSEDRHHSSR-DFHILFVPRRSMLCEQRLKEQGVLGSFINIDEYILDLIPYDGDLLSMEYESAFRECYLENDQTSLYHTAKGLMTLQALYGTIPQIYGKGECARLVANMMLRMKREFAGSQNQILPVFDTLLLLDRNVDLLTPLATQLTYEGLIDEIYGINNGYVKLPPEKFAQKKQGEGSKDLPTEPKKLQLNSAEELYAEIRDKNFNAVGAALSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARSSLANHTSIAELIKDITTSEAFFDNLTVEQEFMTGVDTDKVNTYIEDCIAQKDPLIKILRLVCMQSVCNNGLKHKVMDYYKREILQTYGYEHILTLNNLEKAGLLKPQTGSRNNYPTIRKTLKLWMEDANEQNPNDISYVYSGYSPLSIRLTQILARPGWRSIEEVLKMLPGPHFEERQQLPSGLHKKRQQGENR--TTLVFFLGGVTYAEIAALRFLSQVEDSGMEYVIATTKLVNGTTWIKSLM 592          

BLAST of vacuolar protein sorting-associated protein 33a vs. nr
Match: gi|1079800569|ref|XP_018525212.1| (PREDICTED: vacuolar protein sorting-associated protein 33A [Lates calcarifer])

HSP 1 Score: 610.142 bits (1572), Expect = 0.000e+0
Identity = 294/592 (49.66%), Postives = 415/592 (70.10%), Query Frame = 0

Query:    5 SDSMIQFDLLRDLARRELLDHLDKYAGTKLLVWDDGLTAPLDLIAKHSTLKERQVEKMVPLKPGRLPVDLGQYENVVFVTRPEVHLMEAVADNVKFEEHSHGTRAEFHIMFVPKRSLLCEMRLKEKEVYGSFTFLDELSLFWFPLDTDVISMENRQVFRDFHLNDDPTCLNQVAKGMLALQAVFGIIPRVIGKGHAARKVFEFMTQMRKELVVDEPQVISKIDTLIIIDRQIDLISPFLTQLTYEGLIDEMFSINYNTVKLPAEKFHAPDSSDSEEPNPFGMKVIHLNSQDELYSELRDKNFNAIGPSLSKKARAVSAQMDERHDAKTVQELRLFVDKIPKMKVLRQSLSIHTTIAELVKEATNEKRFLENLKVEQELINFQNADRPLEYLEDACCHDEDIVSVLRLVCLQNVVNSGVKPKVLESYRRLILQTYGYKHLLTLQNLDRLGLLSPQLQTSRNYPVLRKRLNLTRDDVDEQQPNDIAYVHSVYAPLSIRLVQNLAHPGWKTIKDVLDLLPGPTLEEAQKTSSNVKRIKPPGEDHQSTTLVLFVGGCTFAEIAALRYLSQREDSPTEYMVATTAIVNGDTFVKSLL 596
            S   +  ++LR+ AR+EL + LDK +G+K +VWD+ LT P  LIA++S LKE +VEKM  LK GRLP      +N++F  RP + LM+ +A+NV  E+  H +R EFHI+FVP+RS+LCE RLKE+ V GSF  +DE  L   P D D++SME    FR+ +L +D T L   AKG++ LQA++G IP++ GKG  AR V   M +M++E    + Q++   DTL+++DR +DL++P  TQLTYEGLIDE++ IN   VKLP EKF      ++ +  P   K + LNS +ELY+E+RDKNFNA+G +LSKKA+ +SA  +ERH+AKTV E++ FV ++P M+  R SL+ HT+IAEL+K+ T  + F +NL VEQE +   + D+   Y+ED     + ++ +LRLVC+Q+V N+G+K KV + Y+R ILQTYGY+H+LTL NL++ GLL PQ  +  NYP +RK L L  +D +EQ PNDI+YV+S YAPLSIRL Q LA PGW++I++VL +LPGP  EE Q+  + + + +  GE+   TTLV F+GG T+AEIAALR+LSQ EDS  EY++ATT ++NG T++KSL+
Sbjct:    6 SYGRVNLNILREAARKELREFLDKCSGSKAIVWDEYLTGPFGLIAQYSLLKEHEVEKMFTLKSGRLPS--ADVKNIIFFVRPRLELMDIIAENVFSEDKLHSSR-EFHILFVPRRSMLCEQRLKEQGVLGSFINIDEYILDLIPYDGDLLSMEYESAFRECYLENDQTSLYHTAKGLMTLQALYGTIPQIYGKGECARHVANMMLRMKREFAGSQNQILPVFDTLLLLDRNVDLLTPLATQLTYEGLIDEIYGINNGYVKLPPEKFAQKKQGEASKDLPTEPKKLQLNSAEELYAEIRDKNFNAVGAALSKKAKIISAAFEERHNAKTVGEIKQFVSQLPHMQAARSSLANHTSIAELIKDITTSEAFFDNLTVEQEFMTGVDTDKVNTYIEDCIAQKDPLIKILRLVCMQSVCNNGLKQKVFDYYKREILQTYGYEHILTLNNLEKGGLLKPQTSSRNNYPTIRKTLKLWMEDANEQNPNDISYVYSGYAPLSIRLTQVLARPGWRSIEEVLKMLPGPHFEERQQLPAGLHKKRQQGENR--TTLVFFLGGVTYAEIAALRFLSQMEDSGMEYIIATTKLINGTTWIKSLM 592