BLAST of probable 4-coumarate-- ligase 1-like vs. L. salmonis genes
Match: EMLSAG00000001709 (supercontig:LSalAtl2s:LSalAtl2s1296:48150:50137:-1 gene:EMLSAG00000001709 transcript:EMLSAT00000001709 description:"maker-LSalAtl2s1296-augustus-gene-0.17")

HSP 1 Score: 426.402 bits (1095), Expect = 1.679e-143
Identity = 236/538 (43.87%), Postives = 334/538 (62.08%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVG-EKVPG-YHSVSEM--MDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAK 571
            I  S YE+V +       F+WEN + Y  + ALV G+T +   Y+  +  S+ F   L  LG  KGDVM +++PN  E+ + F G   +GVT+TT+NP Y P EIA+QL  S A + +T    L  ++ +     +S I   +KI ++G E+ P    S  EM   D     +    ++ ++DI  LPYSSGTTG PKGV LTH N+V N+ Q  L H  I     +  +  Q  +I VLP +H +AM  ++   L  G KL+ LPKF+P+ FI +L +YKPT + + PPLVSF  + P +KP  L SI+ +   A+  GP+LI+KF +K  S++ ++E +GMTE SP+  +        GSCG L PNT+ K++D++TG+ LGP + GELC+ GPQIM+GY  N +AT + IQ+ W H+GDIA Y+  G+F+IVDRLKELIKVKG QV P+ELEDLIR +  + DVA +GVP E+ GE PRAYVV K   +V E+ I  +V E++A +K L  GV  +D +PK+  GKILR++L A+
Sbjct:   47 IQYSPYEEVVLPSIPYADFIWENLEDYKEREALVCGMTGRTYTYEMVHSLSQKFGSALLRLGAKKGDVMGLVVPNIPEFVIAFMGCASIGVTLTTMNPTYRPEEIARQLDNSGAKYVMTIGLFLQNIKQSCEI--YSGI---QKIIVIGMEETPSDCKSFMEMAIFDDGSFFDQRETIDPHKDILALPYSSGTTGPPKGVALTHSNLVNNITQ--LSHPSIHNF--IGDEVEQHISIAVLPFFHIFAMTCSLSAGLRFGVKLITLPKFEPEMFINSLVQYKPTHMLVVPPLVSFMTSSPLIKPSLLSSIKMLNGGASTFGPTLIEKFMNKCDSDLRFQEGYGMTECSPVTHVQPKDGIIYGSCGNLIPNTIGKIIDIDTGKVLGPGENGELCVSGPQIMQGYYKNKKATDKIIQNGWXHTGDIAYYNQDGQFYIVDRLKELIKVKGLQVAPSELEDLIRKYPGVDDVAXVGVPDERNGELPRAYVVRKN-RSVMEQGIIDFVTEKVAPHKKLGAGVMFVDTLPKNQTGKILRRELKAQ 574          

BLAST of probable 4-coumarate-- ligase 1-like vs. L. salmonis genes
Match: EMLSAG00000002081 (supercontig:LSalAtl2s:LSalAtl2s138:151856:349462:1 gene:EMLSAG00000002081 transcript:EMLSAT00000002081 description:"augustus_masked-LSalAtl2s138-processed-gene-3.0")

HSP 1 Score: 161.77 bits (408), Expect = 5.662e-43
Identity = 125/499 (25.05%), Postives = 235/499 (47.09%), Query Frame = 0

Query:   99 GGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKA----CWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEP------NVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTE------TSPIATMTSLS--------DEHNGSCGKLSPNTVAKVVDLNTGEAL-GPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKY 572
            G +KGDV+ +   N VEY ++  G    G  +  +N    P E+  Q++ SK     C      + L  LE         +    EK+ ++G +      V  M  TDL+ +        V+ +  +D+  +P++S ++G  KG++ T+ +++                  L  D+      C    +H        + ++Y G  +  L +F  + F++AL   K   +      +   +  P V  + L S++ ++        ++  +  D+ PS    REA+G+ E      T P     S++        D+H    G  +  ++ K++    G  + GP+Q+GE+C+K PQ M GYL+  E++A +  D + H+GD+  YD+QG  + V+++  LI+   ++V P+ LE  + G   I D AV+G+P+++ G+ PRA+VV K G   +EE ++  +  R+  ++ + GG+  + ++P+    K+LR  + A+Y
Sbjct:  108 GFVKGDVLTLYCSNFVEYWILALGVWTCGGIVMPVNCELPPDELELQMRESKTKILVCDDFNYVDGLDFLE---------NTNMIEKLIIIGNECTAQDKVILM--TDLLNDDGKKVPQKVIFDWAKDVIYMPFTSTSSGA-KGILHTNKSLMSQFYSPDGAAN--HWFDQLIGDSIH----CANYFFHMTGFYNFALGSIY-GISIYTLSEFSEQNFLEALVDXKTASISCYAWQIRILSQSPLVSNYDLSSLKVLMTGCGLLSSTIRMEVLDRIPSIKYVREAYGLNECGLVTLTYPREKKNSIASSKAADVPDDHIIPMGLPNMYSMIKIISRQDGNPVDGPDQQGEICVKSPQCMLGYLNKEESSAFD-NDQYFHTGDLGYYDNQGIVYFVEKINNLIQFWMYEVAPSILESRLLGSNNIVDAAVVGIPNKENGQVPRAFVVLKPGFEETEENLTNLMESRLQDHERIRGGLYFVQNIPRDENWKVLRS-VLAEY 585          

BLAST of probable 4-coumarate-- ligase 1-like vs. L. salmonis genes
Match: EMLSAG00000009904 (supercontig:LSalAtl2s:LSalAtl2s645:223217:227522:-1 gene:EMLSAG00000009904 transcript:EMLSAT00000009904 description:"maker-LSalAtl2s645-snap-gene-2.24")

HSP 1 Score: 111.309 bits (277), Expect = 9.588e-26
Identity = 104/380 (27.37%), Postives = 176/380 (46.32%), Query Frame = 0

Query:  216 DICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAM-NVTMVPTLYCGGKLVILPKFDPKTFIQALQRYK---PTFLHLAPPLVSFCATHPDVKPHH-------LESIEHIVVAAAPSGPS-LIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHN--GSCGKLSPNTVAKVVDLNTGEALG----------PNQRGELCIKGPQIMKGYLDNPEATAENI--QDDWLHSGDIAIYDDQGRFFIVDRLK-ELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDL 568
            D  ++ Y+SGT+G PKGV +TH NI   V          S +    A T     + VLPLYH + + N  +VP L  G ++ +LP FD K     L         F+ + P + S      ++   +       L S   ++V  + S P  L++K++    S  +  E +GMTE   +    SL ++    G  G   PN  A+++    G  L            ++ GEL I+GP +   Y  N +AT E+     DW  +GD A  + +G F I+ R   ++IK  GF++   E+E     +  I ++AV+G+  E FGE   A +V  +G  ++ + +  +  +++  Y  +     +++ + K+  GKI +K+L
Sbjct:  148 DGALILYTSGTSGSPKGVWITHRNIEAQV----------SGMIKFWAWTSDDHILHVLPLYHLHGLINCLLVP-LSIGARVKMLPGFDAKKVWSYLTNSSMNISVFMGV-PTIYSLLTKEYELDKRNKTEIHDMLRSTYRLMVCGSASLPEPLLRKWQ--TISGHILLERYGMTEIG-MGLGNSLEEDKRLPGFVGTPFPNVEARIMS-EEGSVLAYSNDVEFSYLSDKSGELQIRGPSVFSFYYGNEKATKESFDPNGDWFLTGDTASMNSRGVFKILGRTSADIIKSGGFKLSALEIEREYLSYPDISEIAVVGLEDEIFGEIVAAIIVPNKGYYLNSDILRGFGYKKLPPYS-VPRQFMLVNSIDKNHMGKINKKEL 510          

BLAST of probable 4-coumarate-- ligase 1-like vs. L. salmonis genes
Match: EMLSAG00000007696 (supercontig:LSalAtl2s:LSalAtl2s448:237586:242919:1 gene:EMLSAG00000007696 transcript:EMLSAT00000007696 description:"maker-LSalAtl2s448-augustus-gene-2.14")

HSP 1 Score: 102.449 bits (254), Expect = 3.368e-23
Identity = 130/546 (23.81%), Postives = 224/546 (41.03%), Query Frame = 0

Query:   55 HFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLET-AIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPK---GVMLTHHNIVGNVCQQTLGHRDISTIQPLQA----DTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPK---------------FDPKTFIQALQRYKPTFLHLAPPL-VSFCATHPDVKPHH-LESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRG-FGKIQDVAVIGVPHEKFGEAPRAYVVAKEGE------TVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDL 568
            H  + NK  +   ++  +   +  I+  E  + +K FA  +    G       ++L N   + +   G   +G +++   P  +   I +QL + K    +  +E++   ET AI K+  S+I     I L    VPGY S+ E           + +N + ++  +    GTT +     GV+ +  NI+ ++           +  P Q        +P+++ ++ L + +       P  +  G   ILP+               F       A+Q  KP  +  +P   V       D+     L S+  I         +  KK +++ PS  +  + +G TET  I+    +SD+H    G+L P    ++++ N GE LGPN  G++ +K    M+ Y+ + E      QD + + GD+  YD  G+ F   R K+L+KV    V P E+E  I      ++D+ V G   +   E     +V    E       V E    AY   +   YK L GGV +   +P++A GKI R  L
Sbjct:   49 HLCYSNKGSF---LSYENEHRSNSISPREFVKYTKRFAHFISERHGNARFATVLLLNNTRFFFIPVVGTWFLGSSVSLFQPSSSRETIERQLMILKE--ELNLEEIVVITETFAIPKI--SNIGVDRIISLT--PVPGYMSLQE-----------IFMNDDNEVVEIFNREGTTSLQSEIAGVLYSAENILNSI--------HYCSYVPFQIHHVIKNYEPSSLRLMILTNFFH------PGGFTEGLRNILPQKRTGIILRSPNEYVDFSTTHLFNAIQTTKPNMIICSPEFAVKMNGASNDLSSQFDLSSVFVIYPIGGSINTNYEKKARNQFPSLKMICKGYGSTETLSISL--GISDQH---LGRLFPKYQLRILNPN-GEPLGPNSVGDIVVKPGYGMRRYIGDHEYNI--TQDGFFYMGDVGYYDSAGKIFFKCRSKDLVKVHNHHVYPQEIESFISSKCSFVKDIGVFGQKDKNGIERLSGVIVLHHIEKGLSLQNVLERTFEAY---KFPRYKWLQGGVRVTSAIPRNANGKIFRGRL 549          

BLAST of probable 4-coumarate-- ligase 1-like vs. L. salmonis genes
Match: EMLSAG00000011903 (supercontig:LSalAtl2s:LSalAtl2s83:250927:254806:1 gene:EMLSAG00000011903 transcript:EMLSAT00000011903 description:"maker-LSalAtl2s83-augustus-gene-2.4")

HSP 1 Score: 100.138 bits (248), Expect = 1.853e-22
Identity = 64/197 (32.49%), Postives = 100/197 (50.76%), Query Frame = 0

Query:  374 WGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGP-QIMKGYLDNPEATAENIQDD-WLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDL 568
            + MTE+  +  ++     + G  G    N    + D+++GE LGPN  GEL  + P +   GYL+ P+ T +   DD W  SGD+  Y+D G  F   R+KE++K  G  V P E+E +I+        A +    EK        VV K+G  +  EEI  +V +R+  YKHL   +   + +P++  GK+LR D+
Sbjct:  350 YAMTESGGLILISKDQRSNGGVVG----NMEVYIRDIHSGERLGPNTSGELIYRNPDKPTLGYLNRPDETKKFFLDDGWCCSGDMFHYNDHGELFYEGRIKEMLKSNGSHVYPIEIETIIQSMNSNIIEAGLFSKTEKIQTILGGIVVIKKGYRIDPEEIRQFVNDRIDDYKHLEIIILTNEPLPRNPNGKLLRNDI 542          

BLAST of probable 4-coumarate-- ligase 1-like vs. L. salmonis genes
Match: EMLSAG00000006309 (supercontig:LSalAtl2s:LSalAtl2s343:300230:343872:-1 gene:EMLSAG00000006309 transcript:EMLSAT00000006309 description:"maker-LSalAtl2s343-augustus-gene-3.23")

HSP 1 Score: 98.9821 bits (245), Expect = 1.000e-21
Identity = 92/357 (25.77%), Postives = 161/357 (45.10%), Query Frame = 0

Query:  220 LPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPK--FDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSP---NTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQ-DDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFA 570
            + ++SGTTG+PK V L+H  ++ N          + T   +  D         +PL+H +      + +L  G KLV LP   FDP   +  +   K T+L   P +                   +I V  A       KK           R  +G TET P++ +    D        + P   +  AK+VD N G+ L  N  GEL ++ P +M GY  + +++ + I  D + HSGD+ +  ++G   I+ RL +++   G  + P E+ED +     I +V ++GVP +  GE   A++  +EG    E ++  + + ++A +K     + + D  PK+ +GK+ + ++ A
Sbjct:    4 IQFTSGTTGLPKAVALSHFCVINNA---------LLTGNRIGFDLKYHXINLWVPLFHTFGCVTGTLISLCYGSKLV-LPSDGFDPTACLNCIPAEKCTYLFGTPTM-------------------YIAVLEAQ------KKL----------RNIYGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQN-GKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDFLLKHPSIVEVYIVGVPDDYMGEELCAWICLEEG--AKEFDLKEHCKGKIAHFKIPKYPMFVKD-FPKTLSGKVKKFEMKA 311          

HSP 2 Score: 83.1889 bits (204), Expect = 9.322e-17
Identity = 58/199 (29.15%), Postives = 105/199 (52.76%), Query Frame = 0

Query:  374 WGMTETSPIATMTSLSDEHNGSCGKLSP---NTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQ-DDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDL 568
            +G TET P   + S  D        + P   +  AK+VD N G+ L  N  GEL ++ P +M GY  + +++ + I  D + HSGD+A+  ++G   I+ RL +++   G  + P E+ED +     I +V +IGVP +  GE   A++  +EG    E ++  + + ++A +K     + + D  PK+ +GK+ + ++
Sbjct:  714 YGATETGPATFVPSPEDSFEVRINTVGPPLGHVEAKIVDQN-GKILPVNTPGELWVRTPGLMHGYYKDQKSSQKAIGPDRYYHSGDLAMITEKGYGKIIGRLDDMVNRGGENIYPKEIEDFLLKHPNIVEVYIIGVPDDYMGEELCAWICLEEG--AKEFDLKEHCKGKIAHFKIPKYSMFVKD-FPKTLSGKVKKLEM 908          

HSP 3 Score: 77.7962 bits (190), Expect = 4.095e-15
Identity = 55/201 (27.36%), Postives = 106/201 (52.74%), Query Frame = 0

Query:  374 WGMTETSPIATMTSLSDEHNGSCGKLSP---NTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQ-DDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFA 570
            +G TET P++ +    D        + P   +  AK+VD N G+ L  N  GEL ++ P +M GY  + +++ + I  D + HSGD+ +  ++G   I+ RL +++   G  + P E+ED++     I +V ++GVP    GE   A++  +EG    E ++  + + +++ +K     + + D  PK+ +GK+ + ++ A
Sbjct:  343 YGATETGPVSFIACPKDSFEVRINTVGPPLGHIEAKIVDQN-GKILPVNTPGELWVRTPGLMHGYYKDQKSSKKVIGPDRYYHSGDLGMITEKGYCKIIGRLDDMVIRGGENIYPKEIEDVLLKHPSIVEVYIVGVPDNYMGEELCAWICLEEG--AKEFDLKEHCKGKISHFKIPKYPMFVKD-FPKTLSGKVKKFEMKA 539          

BLAST of probable 4-coumarate-- ligase 1-like vs. L. salmonis genes
Match: EMLSAG00000010401 (supercontig:LSalAtl2s:LSalAtl2s68:357669:363984:1 gene:EMLSAG00000010401 transcript:EMLSAT00000010401 description:"maker-LSalAtl2s68-augustus-gene-3.30")

HSP 1 Score: 92.8189 bits (229), Expect = 3.080e-20
Identity = 61/178 (34.27%), Postives = 94/178 (52.81%), Query Frame = 0

Query:  395 SCGKLSPNTVAKVVDLNTGEALGPNQRGELCI-KGPQIMKGYLDNPEATAENIQDD-WLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGF-GKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDH-VPKSAAGKILRKDL 568
            S G +   T   + DLNTG+ LGPN+ GEL   K      GYL+  E   +  + D W H+GD   Y +QG FF   R+ E+IK +G  V P ELE L++     I +V V+ V  EK      A +V K  + ++ E I   + +++  YK + G + +    +P++A GK+LR ++
Sbjct:  307 SNGGVVARTETYIRDLNTGQHLGPNKIGELMFRKANATSTGYLNRSEENEKMFEKDGWTHTGDFFHYTEQGEFFFHSRINEMIKCQGSHVYPIELEGLLKAMHDDIIEVGVVPVV-EKKQTVLGAIIVVKNDKKINVETIRKELNKKVDEYKRIEGQLLLTKEPLPRNAMGKLLRSEI 483          

BLAST of probable 4-coumarate-- ligase 1-like vs. L. salmonis genes
Match: EMLSAG00000003336 (supercontig:LSalAtl2s:LSalAtl2s182:521806:564306:1 gene:EMLSAG00000003336 transcript:EMLSAT00000003336 description:"maker-LSalAtl2s182-augustus-gene-5.28")

HSP 1 Score: 90.8929 bits (224), Expect = 3.656e-19
Identity = 121/492 (24.59%), Postives = 207/492 (42.07%), Query Frame = 0

Query:   80 NYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPL-----IFAGGPGVGVTITT--------LNPIYTPHEIAKQLQ----MSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPK-TFIQALQRYKPTFLHLAPPL-------------------VSFCATHPDVKPHHLESIEHIVV----------------------AAAPSGPSLIKKFKDKAPS-NVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQ-VPPAELEDLIRGFGKIQDVAVIGVPHEKF 510
            NYD+ Y   K  A     LG      + +   NC E+ +     IFAGG   G+  T         +N       + + LQ    +S     +TT + +  +E    + G + I WK+ +  +G K  G   +++ +           ++VN+  C L Y+SGTTG PKGVML+H +I+  + +  +   D +T + +         +  LPL H  A  + +   LY  G++    K+  K + +  L+  +PT     P +                   +       D   H+ E+ +  V+                           G  L ++ +D   S ++  +E +G++E     ++   SD   G+CG + P+T AKVV  ++ + +G    GE+CIKG  +M GYL N +AT+E              +D +G +F   RLK+L+   G + V PA +E+ I+ +  +   AV+    EKF
Sbjct:   76 NYDQYYSDIKLTAKAFIKLGLDPFYTVCIYSFNCPEWFISNLGAIFAGGLSAGLYPTNSSSTNEYLMNNSRCQILVVENLQVLRTISHILKNVTTLKKIVVIEEDGFRPGENLILWKD-LLELGRK-EGIDRLNDRLSH---------ISVNQ-CCSLIYTSGTTGNPKGVMLSH-DIITFMTKAIVKKHDWTTAKFI--------VVSYLPLSHIAASFLDIYVALYLKGEVYFADKYALKGSLVDTLKEVRPTMFFGVPRVWEKFQEGIKEQSKNQKKSQMKVAQMFKDASMHYYENGDQKVIHKIGDKLIFQKVKQALGLDRCLEFISGGAPLSERTRDFFLSLDIPVQECYGLSECGIFCSVQK-SDFKTGTCGTIIPSTHAKVVK-SSDDDIG----GEICIKGRCVMMGYLFNEQATSE-------------AFDKEG-YFHTGRLKDLLLTSGGENVAPAPIENAIKHYLPVVSNAVVVGDAEKF 526          

BLAST of probable 4-coumarate-- ligase 1-like vs. L. salmonis genes
Match: EMLSAG00000010073 (supercontig:LSalAtl2s:LSalAtl2s663:674899:681644:1 gene:EMLSAG00000010073 transcript:EMLSAT00000010073 description:"maker-LSalAtl2s663-augustus-gene-6.10")

HSP 1 Score: 85.1149 bits (209), Expect = 1.464e-17
Identity = 115/519 (22.16%), Postives = 200/519 (38.54%), Query Frame = 0

Query:   63 KYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIF----LVGEKVPGYH------SVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMV-----------------------------------PTLYCGGKLVI-------------LPKFDPKTFIQALQRYKPTF--LHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPS--NVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEAL---GPNQRGELCIKGPQIMKGYLDNPEATAENIQDD-----WLHSGDIAIYDDQGRFFIVDRLKELIKVK-GFQVPPAELEDLIRGFGKIQDVAVIGVPHEKF 510
            K F K A+ +    Q  +++E    S +F   L+ LG    + + +   +  E+     G       + TL        IA  +  ++    ITT +LLP L+  + +     I++   IF    L      GY       S  E+++     E        +D  ++ Y+SG+TGVPKGV+L H +++G +    L  +D           P+ T +  LPL H Y +   M                                    PTL C   LVI               KF  ++ +     YK  +  L +  P+++     P ++      +  IV   AP    L     D      ++   + +G+TE+    ++    +   G  G      + ++++   G       P  RGE+ I GP I  GY   P+ TAE+   D     W  +GD+  + + G   I+DR K+L+K++ G  V   ++E +I+    +++V   G P + +
Sbjct:  130 KVFKKWAMGE---YQWKSFNEIEELSTDFGKGLRELGLETKENICIFAESKAEWMXSALGCFKQTFPLVTLYANLGEDAIAHGVNQTQVSHLITTHDLLPKLKNVLERT--PSIKYI--IFFEDQLNATDRSGYKXGVRILSFQEVIELGSKSETKATPPTKDDPAIIMYTSGSTGVPKGVILPHESLIGTIAAFHLVVKD---------KIPEETYLGYLPLAHIYELLAEMTMLIHGIKVGYSSPNTMIDKSTKVMKGSKGDCSVLRPTLMCAVPLVIDRIYKGIHENLSKKSKF-MRSLLHFCYHYKSHYRRLGMRTPIMNALIFRP-MRAIVGGKVRVIVSGGAP----LSHNIHDYVSCALDIPIHQGYGLTESCAGGSVMHREELSTGRVGPPGQGVLIRLINWEDGGYRVTDKPRPRGEIVIGGPNIAHGYYKLPQKTAEDFYKDENNVRWFKTGDVGEFAEDGTLKIIDRKKDLVKLQYGEYVSLGKVETVIKTCPLVENVCAYGDPTKSY 626          

BLAST of probable 4-coumarate-- ligase 1-like vs. L. salmonis genes
Match: EMLSAG00000004934 (supercontig:LSalAtl2s:LSalAtl2s259:871050:904452:1 gene:EMLSAG00000004934 transcript:EMLSAT00000004934 description:"augustus_masked-LSalAtl2s259-processed-gene-9.9")

HSP 1 Score: 79.337 bits (194), Expect = 1.009e-15
Identity = 112/516 (21.71%), Postives = 214/516 (41.47%), Query Frame = 0

Query:   77 QQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVG---HSDIRWKEKIFLVGEKVP----GYHSVSEMMDTDLVG----------EPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTM------VPTLYCG-GKLVILPKFDPKTFIQALQRYKPTFLHLAPP----------------------LVSFCATH-----------PDVKPHHLESIEHIV---VAAAPSGPSLIKKFKDKAPSNVVY----REAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEAL---GPNQRGELCIKGPQIMKGYLDNPEATAENIQDD----WLHSGDIAIYDDQGRFFIVDRLKELIKVK-GFQVPPAELE---------DLIRGFGKIQDVAVIG--VPHEK 509
            + + Y++    S+NF+  L+ L  +  D + +   +  ++ +   G     +T+ T+        +   ++ + A   IT++ LL  L+  +A  G    + I  +++ F + +  P      +S +E+++  L             PN     +EDI V+ Y+SG++GVPKGV+L+H NI+ N+     G         ++ D  + + I  LPL H + ++V +      +P  + G   L+       +  I  +   KPT++ + P                       L +FC  +           P +     + I+  +   V+   SG + +   +      +V+     + +G+TET     +    D   G  G   P    K+ + + GE      P  RGE+ + G  I  GY +  + T EN  ++    W  +GDI      G   I+DR K++IK++ G  V   ++E         D+I  +G+ +   ++G  +P+EK
Sbjct:  212 EWLTYEDVNTISRNFSKGLRHLKFLPKDKILLYAESSAKWLVSAFGCFKNSMTVVTIYTNLGDEGVYHCIEQTDAKVIITSQNLLKRLQKILASKGGLIQTIIYLEKEEFSLAQTTPEQDVNVYSFNEILEKGLKSYSQNDNEEDWRPN-----SEDIAVIMYTSGSSGVPKGVVLSHFNIMSNI----FGIMSFLNQNFVRLD--KESYIGYLPLAHIFELSVEIALFGKGIPIGFSGPNTLMNSGSMVKEGQIGDICELKPTYMAVVPLIADRIYKIIIQKVSENGPFSELLFNFCYEYKSKWRKLGMNTPIINLLFFKKIQQFLGGNVSTIVSGGAPLSA-QAHEFMEIVFDLKLSQGYGLTETCGGFILGRKEDFSLGEVGHPFPGPFVKLTNWDEGEYTVKDKPYPRGEIHVGGGLIAMGYFELMDETEENFYEENGVRWFKTGDIGQLQSSGALKIIDRKKDIIKLQNGEYVSLGKVETYLGTHPLVDIICVYGEPERNCIVGLIIPNEK 715          

BLAST of probable 4-coumarate-- ligase 1-like vs. SwissProt
Match: gi|75290242|sp|Q6ETN3.1|4CL3_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 3; Short=4CL 3; Short=Os4CL3; AltName: Full=4-coumaroyl-CoA synthase 3)

HSP 1 Score: 344.739 bits (883), Expect = 1.334e-110
Identity = 204/554 (36.82%), Postives = 297/554 (53.61%), Query Frame = 0

Query:   38 IVRSQYEDVPISDK-SLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQ-------MSKACWAITTKELLPTLETAIAKV-GHSD--IRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMT-SLSDE----HNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDD-WLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMA 574
            + RS+  D+ I +  +L  + +    +   +  L+DG T +   Y E    S+  A  L+ +G  KGDV+  +L NC E+   F G   +G   TT NP YTPHE+ +Q +       +++AC     +E        +  V G  D  + ++E +             +E +D D    P+       D+  LPYSSGTTG+PKGVMLTH +++ +V QQ  G        P    +     +C+LPL+H Y++N  ++  L  G  +VI+ KFD    +  ++++  T     PP+V   A  P V    L SI  ++  AAP G  L   F  K P N V  + +GMTE  P+  M  + + E     +GSCG +  N   K+VD +TG +LG NQ GE+CI+G QIMKGYL++PEAT   I +D WLH+GDI   DD    FIVDRLKE+IK KGFQVPPAELE L+    +I+D AV+ +  +  GE P A++V  EG  ++E+EI  +V + +  YK  +  V   D +PK+ +GKILRKDL A+  A
Sbjct:   11 VFRSKLPDIEIDNSMTLQEYCFARMAEVGARPCLIDGQTGESYTYAEVESASRRAAAGLRRMGVGKGDVVMSLLRNCPEFAFSFLGAARLGAATTTANPFYTPHEVHRQAEAAGARVIVTEACAVEKVREFAAERGVPVVTVDGAFDGCVEFREVL------------AAEELDADADVHPD-------DVVALPYSSGTTGLPKGVMLTHRSLITSVAQQVDGE------NPNLYFSKDDVILCLLPLFHIYSLNSVLLAGLRAGSTIVIMRKFDLGALVDLVRKHNITIAPFVPPIVVEIAKSPRVTAEDLASIRMVMSGAAPMGKDLQDAFMAKIP-NAVLGQGYGMTEAGPVLAMCLAFAKEPFKVKSGSCGTVVRNAELKIVDPDTGTSLGRNQSGEICIRGEQIMKGYLNDPEATKNTIDEDGWLHTGDIGFVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPEIKDAAVVSMKDDLAGEVPVAFIVRTEGSEITEDEIKKFVAKEVVFYKR-INKVFFTDSIPKNPSGKILRKDLRARLAA 537          

BLAST of probable 4-coumarate-- ligase 1-like vs. SwissProt
Match: gi|1168218|sp|P41636.1|4CL_PINTA (RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName: Full=4-coumaroyl-CoA synthase)

HSP 1 Score: 343.969 bits (881), Expect = 1.615e-110
Identity = 202/548 (36.86%), Postives = 310/548 (56.57%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFH-FMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVP--GYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHN------GSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDD-WLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMAE 575
            + RS+  D+ ISD    H + +E   ++  +  L+DG T++   + E    S+  A  L  LG  +G V+ ++LPNC+E+  +F G    G  +TT NP Y P EIAKQ + + A        ++ TL   + K+  +D++  + + +  +  P  G   +S + + D    P V ++  +D+  LPYSSGTTG+PKGVMLTH  +V +V QQ  G        P          +CVLPL+H Y++N  ++  L  G   +I+ KF+  T ++ +Q+YK T   + PP+V      P V  + + S+  I+  AAP G  L    +++ P   ++ + +GMTE  P+  M +L+   N      GSCG +  N   K++D  TGE+L  NQ GE+CI+GP+IMKGY+++PE+TA  I ++ WLH+GD+   DD    FIVDR+KE+IK KGFQV PAELE L+     I D AV+   HE+ GE P A+VV  +   +SE+EI  +V +++  YK  +  V  +D +PKS +GKILRKDL ++  A+
Sbjct:   11 LYRSKLPDIEISDHLPLHSYCFERVAEFADRPCLIDGATDRTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVFMGASVRGAIVTTANPFYKPGEIAKQAKAAGA-------RIIVTLAAYVEKL--ADLQSHDVLVITIDDAPKEGCQHISVLTEADETQCPAVKIH-PDDVVALPYSSGTTGLPKGVMLTHKGLVSSVAQQVDGE------NPNLYFHSDDVILCVLPLFHIYSLNSVLLCALRAGAATLIMQKFNLTTCLELIQKYKVTVAPIVPPIVLDITKSPIVSQYDVSSVRIIMSGAAPLGKELEDALRERFP-KAIFGQGYGMTEAGPVLAM-NLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGESLPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVEYIDDDEEIFIVDRVKEIIKYKGFQVAPAELEALLVAHPSIADAAVVPQKHEEAGEVPVAFVV--KSSEISEQEIKEFVAKQVIFYKK-IHRVYFVDAIPKSPSGKILRKDLRSRLAAK 537          

BLAST of probable 4-coumarate-- ligase 1-like vs. SwissProt
Match: gi|75289194|sp|Q67W82.1|4CL4_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 4; Short=4CL 4; Short=Os4CL4; AltName: Full=4-coumaroyl-CoA synthase 4)

HSP 1 Score: 340.117 bits (871), Expect = 7.228e-109
Identity = 216/550 (39.27%), Postives = 295/550 (53.64%), Query Frame = 0

Query:   38 IVRSQYEDVPI-SDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKV-GHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNE-----DICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMT-SLSDE----HNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENI-QDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMA 574
            + RS+  D+ I S  +L  + +E   +   +  L+DG T    +Y E    S+  A  L+ LG  KGDV+  +L NC E+   F G   +G   TT NP YTPHEI +Q   + A        ++ T   A+ KV G +  R    + + G+          M+D  +  EP   L+ +E     D+  LPYSSGTTG+PKGVMLTH ++V +V QQ  G        P      +   +C+LPL+H Y++N  ++  L  G  +VI+ KFD    +   +R+  T     PP+V   A  P V    L SI  ++  AAP G  L   F  K P N V  + +GMTE  P+  M  + + E     +GSCG +  N   K+VD +TG  LG NQ GE+CI+G QIMKGYL++PE+T   I +  WLH+GDI   DD    FIVDRLKE+IK KGFQVPPAELE L+     I+D AV+ +  E  GE P A++V  EG  +SE EI  +V + +  YK L   V   D +PKS +GKILRKDL AK  A
Sbjct:   18 VFRSKLPDIEIPSHLTLQAYCFEKLPEVAARPCLIDGQTGAVYSYGEVEELSRRAAAGLRRLGVGKGDVVMSLLRNCPEFAFTFLGAARLGAATTTANPFYTPHEIHRQASAAGA-------RVIVTEACAVEKVRGFAADRGIPVVAVDGDFDGCVGFGEAMLDASI--EP---LDADEEVHPDDVVALPYSSGTTGLPKGVMLTHRSLVTSVAQQVDGE------NPNLYFRREDVVLCLLPLFHIYSLNSVLLAGLRAGSAIVIMRKFDLGALVDLTRRHGVTVAPFVPPIVVEIAKSPRVTADDLASIRMVMSGAAPMGKDLQDAFMAKIP-NAVLGQGYGMTEAGPVLAMCLAFAKEPFEVKSGSCGTVVRNAELKIVDPDTGATLGRNQSGEICIRGEQIMKGYLNDPESTKNTIDKGGWLHTGDIGYVDDDDEIFIVDRLKEIIKYKGFQVPPAELEALLITHPDIKDAAVVPMIDEIAGEVPVAFIVRIEGSAISENEIKQFVAKEVVFYKRL-NKVFFADSIPKSPSGKILRKDLRAKLAA 547          

BLAST of probable 4-coumarate-- ligase 1-like vs. SwissProt
Match: gi|148841518|sp|Q9S725.2|4CL2_ARATH (RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName: Full=4-coumarate--CoA ligase isoform 2; Short=At4CL2; AltName: Full=4-coumaroyl-CoA synthase 2)

HSP 1 Score: 336.265 bits (861), Expect = 2.275e-107
Identity = 201/552 (36.41%), Postives = 299/552 (54.17%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFH-FMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLE-----------TAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMT-SLSDE----HNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENI-QDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAK 571
            I RS+  D+ I +    H +++EN  ++  K  L++G T +   Y + +  S+  A  L +LG  + DV+ ++LPN  E  L F     +G   T+ NP +TP EI+KQ + S A   +T    +  ++           T    +  + +R+ E   L   + P   S+ E +               ED+  LP+SSGTTG+PKGVMLTH  +V +V QQ  G        P          +CVLP++H YA+N  M+ +L  G  ++I+PKF+    ++ +QR K T   + PP+V   A  P+ + + L S+  +   AAP G  L      K P N    + +GMTE  P+  M+   + E     +G+CG +  N   K++D +TG++L  N+ GE+CI+G QIMKGYL++P ATA  I +D WLH+GD+   DD    FIVDRLKELIK KGFQV PAELE L+ G  +I DVAV+ +  E  GE P A+VV  +   +SE+EI  +V +++  YK  +  V   D +PK+ +GKILRKDL A+
Sbjct:   22 IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSE---LTQSEEPRVDSIPEKISP-------------EDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGE------NPNLYFNRDDVILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRMVKSGAAPLGKELEDAISAKFP-NAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVRSKDSNISEDEIKQFVSKQVVFYKR-INKVFFTDSIPKAPSGKILRKDLRAR 549          

BLAST of probable 4-coumarate-- ligase 1-like vs. SwissProt
Match: gi|12229631|sp|O24145.1|4CL1_TOBAC (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 329.331 bits (843), Expect = 8.854e-105
Identity = 198/544 (36.40%), Postives = 292/544 (53.68%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFH-FMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMT-----SLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENI-QDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMA 574
            I RS+  D+ I      H + +EN  ++  +  L++G  +Q   Y E     +  A+ L  LG  + D + ++LPN  E+   F G   +G   T  NP++TP E+ KQ + S A       +++ T    + KV         K+  +     G    SE+  +D    P V +   +D+  LPYSSGTTG+PKGVMLTH  +V +V QQ  G      +        +   +CVLPL+H Y++N  ++  L  G  ++I+ KFD   F++ +Q+YK +     PP+V   A  P V  + L S+  ++  AAP G  L    + K P N    + +GMTE  P+  M         D  +G+CG +  N   K+VD +TG +L  NQ GE+CI+G QIMKGYL++PEAT   I ++ WLH+GDI   D+    FIVDRLKELIK KGFQV PAE+E L+     I D AV+ +  E+ GE P A+VV   G  ++E+E+  ++ +++  YK  V  V  ++ VPKS +GKILRKDL A+  A
Sbjct:   16 IFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASSA-------KIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQ-PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHS------EDVLMCVLPLFHIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSVRTVMSGAAPLGKELEDAVRTKFP-NAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKR-VKRVFFVETVPKSPSGKILRKDLRARLAA 543          

BLAST of probable 4-coumarate-- ligase 1-like vs. SwissProt
Match: gi|112800|sp|P14912.1|4CL1_PETCR (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 327.405 bits (838), Expect = 3.716e-104
Identity = 209/541 (38.63%), Postives = 294/541 (54.34%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFH-FMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMT-SLSDE----HNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDD-WLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAK 571
            I RS+  D+ I      H + +EN  K   K  L++G T +   Y +    S+  A  L  LG  +GD + ++LPN  EY   F G    G   T  NP +T  E+ KQL+ S+A       +L+ T    + KV         +I  + +        S++M+ D    P VV+N ++D+  LPYSSGTTG+PKGVMLTH  +V +V QQ  G        P      +   IC+LPL+H Y++N  +   L  G  ++I+ KFD   F++ +Q+YK T     PP+V   A  P V  + L S+  ++  AAP G  L    + K P N    + +GMTE  P+  M  + + E     +G+CG +  N   K+VD  T  +L  NQRGE+CI+G QIMKGYL++PE+T   I ++ WLH+GDI   DD    FIVDRLKE+IK KGFQV PAELE L+     I D AV+ +  EK GE P A+VV   G T +EEEI  +V +++  YK +   V  +D +PKS +GKILRKDL A+
Sbjct:   12 IFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQA-------KLIITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIN-SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGD------NPNLYMHSEDVMICILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFP-NAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIF-RVFFVDAIPKSPSGKILRKDLRAR 536          

BLAST of probable 4-coumarate-- ligase 1-like vs. SwissProt
Match: gi|75294215|sp|Q6ZAC1.1|4CL5_ORYSJ (RecName: Full=Probable 4-coumarate--CoA ligase 5; Short=4CL 5; Short=Os4CL5; AltName: Full=4-coumaroyl-CoA synthase 5)

HSP 1 Score: 326.25 bits (835), Expect = 1.127e-103
Identity = 198/544 (36.40%), Postives = 296/544 (54.41%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFH-FMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATM-TSLSDE----HNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENI-QDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMA 574
            + RS+  D+ I D    H +++E       +  L+DG T + +++ +    S+  A  L S+G   G  + ++LPN VE+ + F     +G   TT NP++TP EIAKQ+  S A   +T    +  + + +A V          +   G       S + +   D    P V ++V  D   LPYSSGTTG+PKGVML+H  +V +V Q   G        P          +CVLP++H Y+++  ++  +  G  +V++ +FD    +Q ++R+  T   L PP+V   A    +  H L SI  ++  AAP G  L      K P N V  + +GMTE  P+ +M  + + E     +G+CG +  N   K+VD +TG +L  NQ GE+CI+G QIMKGYL+NPEAT + I +D WLH+GDI   DD    FIVDRLKELIK KGFQV PAELE ++     + D AV+ +  +  GE P A+VVA++G  ++++EI  YV +++  YK L   +  +D +PK+ +GKILRKDL AK  A
Sbjct:    9 VFRSRLPDIAIPDHLPLHDYVFERLADRRDRACLIDGATGETLSFGDVDALSRRVAAGLSSIGVCHGSTVMLLLPNSVEFAVAFLASSRLGAVTTTANPLHTPPEIAKQVAASGATVVVTEPAFVAKV-SGLAGV---------TVVATGGGAERCASFAGLAAADGSALPEVAIDVANDAVALPYSSGTTGLPKGVMLSHRGLVTSVAQLVDGE------NPNLHLREDDVVLCVLPMFHVYSLHSILLCGMRAGAAIVVMKRFDTVKMLQLVERHGVTIAPLVPPIVVEMAKSDALDRHDLSSIRMVISGAAPMGKELQDIVHAKLP-NAVLGQGYGMTEAGPVLSMCMAFAKEPTPVKSGACGTVVRNAELKIVDPDTGLSLPRNQPGEICIRGKQIMKGYLNNPEATEKTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLIAHAAVADAAVVPMKDDSCGEIPVAFVVARDGSGITDDEIKQYVAKQVVFYKRL-HKIFFVDAIPKAPSGKILRKDLRAKLAA 534          

BLAST of probable 4-coumarate-- ligase 1-like vs. SwissProt
Match: gi|112801|sp|P14913.1|4CL2_PETCR (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 325.479 bits (833), Expect = 2.605e-103
Identity = 209/541 (38.63%), Postives = 293/541 (54.16%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFH-FMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMT-SLSDE----HNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDD-WLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAK 571
            I RS+  D+ I      H + +EN  K   K  L++G T +   Y +    S+  A  L  LG  +GD + ++LPN  EY   F G    G   T  NP +T  E+ KQL+ S A       +L+ T    + KV         +I  + +        S++M+ D    P VV++ ++D+  LPYSSGTTG+PKGVMLTH  +V +V QQ  G        P      +   IC+LPL+H Y++N  +   L  G  ++I+ KFD   F++ +Q+YK T     PP+V   A  P V  + L S+  ++  AAP G  L    + K P N    + +GMTE  P+  M  + + E     +G+CG +  N   K+VD  T  +L  NQRGE+CI+G QIMKGYL++PE+T   I ++ WLH+GDI   DD    FIVDRLKE+IK KGFQV PAELE L+     I D AV+ +  EK GE P A+VV   G T +EEEI  +V +++  YK +   V  +D +PKS +GKILRKDL AK
Sbjct:   12 IFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLA-------KLIITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVID-SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGD------NPNLYMHSEDVMICILPLFHIYSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSGAAPLGKELEDAVRAKFP-NAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIF-RVFFVDAIPKSPSGKILRKDLRAK 536          

BLAST of probable 4-coumarate-- ligase 1-like vs. SwissProt
Match: gi|398965|sp|P31685.1|4CL2_SOLTU (RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName: Full=4-coumaroyl-CoA synthase 2)

HSP 1 Score: 324.324 bits (830), Expect = 7.168e-103
Identity = 206/544 (37.87%), Postives = 297/544 (54.60%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFH-FMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMT-----SLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDD-WLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMA 574
            I RS+  D+ I      H + +EN  ++  +  L+DG  ++   Y E    S+  A+ L  LG  + D + ++LPNC E+   F G   +G   T  NP++TP E+ KQ + S A   IT             KV    I    K+  V     G    SE++ +D    P+V +   +D+  LPYSSGTTG+PKGVMLTH  +V +V QQ  G    +    + +D      +CVLPL+H Y++N  ++  L  G  ++I+ KFD   F++ + ++K T     PP+V   A  P V  + L S+  ++  AAP G  L    + K P N    + +GMTE  P+  M         D  +G+CG +  N   K+VD +TG +L  NQ GE+CI+G QIMKGYL++PEATA  I+ + WLH+GDI   DD    FIVDRLKELIK KGFQV PAELE L+     I D AV+ +  E+ GE P A+VV   G T++E+E+  ++ +++  YK  +  V  ++ VPKS +GKILRKDL A+  A
Sbjct:   14 IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVITQA-------CFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDVKIQ-PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGE---NANLYMHSDD---VLMCVLPLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDLSSVRTVMSGAAPLGKELEDAVRAKFP-NAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKR-IKRVFFVETVPKSPSGKILRKDLRARLAA 541          

BLAST of probable 4-coumarate-- ligase 1-like vs. SwissProt
Match: gi|398963|sp|P31684.1|4CL1_SOLTU (RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName: Full=4-coumaroyl-CoA synthase 1)

HSP 1 Score: 323.939 bits (829), Expect = 8.973e-103
Identity = 206/544 (37.87%), Postives = 297/544 (54.60%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFH-FMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMT-----SLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDD-WLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMA 574
            I RS+  D+ I      H + +EN  ++  +  L+DG  ++   Y E    S+  A+ L  LG  + D + ++LPNC E+   F G   +G   T  NP++TP E+ KQ + S A   IT             KV    I    K+  V     G    SE++ +D    P+V +   +D+  LPYSSGTTG+PKGVMLTH  +V +V QQ  G    +    + +D      +CVLPL+H Y++N  ++  L  G  ++I+ KFD   F++ + ++K T     PP+V   A  P V  + L S+  ++  AAP G  L    + K P N    + +GMTE  P+  M         D  +G+CG +  N   K+VD +TG +L  NQ GE+CI+G QIMKGYL++PEATA  I+ + WLH+GDI   DD    FIVDRLKELIK KGFQV PAELE L+     I D AV+ +  E+ GE P A+VV   G T++E+E+  ++ +++  YK  +  V  ++ VPKS +GKILRKDL A+  A
Sbjct:   14 IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVITQA-------CFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDEHEIPDVKIQ-PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGE---NANLYMHSDD---VLMCVLPLFHIYSLNSVLLCALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSVRTVMSGAAPLGKELEDAVRAKFP-NAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKR-IKRVFFVETVPKSPSGKILRKDLRARLAA 541          

BLAST of probable 4-coumarate-- ligase 1-like vs. nr
Match: gi|1325315469|ref|XP_023339433.1| (4-coumarate--CoA ligase 2-like [Eurytemora affinis])

HSP 1 Score: 453.751 bits (1166), Expect = 5.382e-150
Identity = 243/541 (44.92%), Postives = 345/541 (63.77%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWK-EKIFLVGEKVPGYHSVSEM-----MDTDLVGEPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKA-PSNVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAK 571
            IV+S++ DV I + +L  ++WE+ +      ALV G+T +  ++  ++  SK F   L  +G  KGDV+ ++LPN  E+P++F G  GV VT+TT+NP Y P EIA+Q++ S+  + +T    +  ++ +       DI    EKI ++G+ +     +S +      D  L G+ +   N +ED+  LPYSSGTTG PKGV LTH+N+V N+CQ   GH D   I+P +      +T+ VLP +H YAM + M+  L  G K + LPKF+P+++I+AL  YKPTFL+L PPLVSF AT+P VKP  L S+  +   AAP GP+LI+KF +K  P  + +RE +GMTE+SP+  +    D   G+CG    NT AK+VD  TGE LGP ++GELC+ GPQ+M+GY  N  AT + I + WL +GDIA YD  G+F IVDRLKELIKVKG QV P+ELEDLIR    + DVAV+GVP E+ GE PRAY++ K    V+E+ I  YV +R+A +K L  GV  ++ +PK+  GKILR++L A+
Sbjct:   60 IVKSRWSDVQIPEMNLADYVWEDIEHNAANPALVCGMTGRSYSFAMAHGLSKKFGSALLRMGAKKGDVIGLVLPNIPEFPIVFMGAVGVNVTVTTMNPTYRPEEIARQMENSETKFIVTIGMFVQNIKQS------CDIYTGVEKIIVIGDAIAPEGCISFLDLIMGEDGSLYGQ-DKTCNPHEDVVALPYSSGTTGPPKGVCLTHYNLVANLCQA--GHPDALPIKPFKETGVLGSTLAVLPFFHIYAMELVMLFNLRNGQKTITLPKFEPESYIKALDTYKPTFLNLVPPLVSFLATNPAVKPSLLNSVTFVTGGAAPFGPALIEKFMEKVHPHQIEFREGFGMTESSPLTHLQPDGDAVLGACGCPVSNTWAKIVDTETGEILGPEEQGELCVSGPQVMQGYFKNISATEKTIINGWLMTGDIAKYDKSGQFTIVDRLKELIKVKGLQVAPSELEDLIRRHSGVLDVAVVGVPDERSGELPRAYIIRKN-RNVTEQSIIDYVADRVAPHKKLGAGVMFVETLPKNQTGKILRRELKAQ 590          

BLAST of probable 4-coumarate-- ligase 1-like vs. nr
Match: gi|1228407097|ref|XP_021962746.1| (probable 4-coumarate--CoA ligase 1 [Folsomia candida])

HSP 1 Score: 431.795 bits (1109), Expect = 1.108e-141
Identity = 244/558 (43.73%), Postives = 340/558 (60.93%), Query Frame = 0

Query:   28 SLSISNGRFGIVRSQYEDVPISDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVG----EPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHN--GSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQ-DDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMAEQAK 578
            S    + RF +V S +   P+   ++  F W N  K+ +  A   GV+ ++  +D+ Y++S++FA  L+  G  + DV+A++LPN  EY  I  G    GVT +  NP YT HEIA QL   +A   +T    LP ++ A A+         + + +VGE  PG HS  EM   +  G    + + +  VN    +LP+SSGTTG PKGVMLTH NI  N+ Q T         +P      Q   I +LP +H Y   + M  TLY G   + LPKF+PK+FI +++ ++P FLHL PPL+SF A  PDVK   L+SI  I+  AAP GP +I +  +KA  + ++ E +GMTE SP++ ++ ++  +   GSCG   P ++ KVVD+ TGEALGPNQ GELCIKGPQ+MKGY  N +AT E I    WLH+GDI +YD+   FFIVDRLKELIKV+GFQV P+ELEDL+R   ++ DVAVIGVP E+ GE PRAY+V+K     SEE I  Y+  +++  K L GGVE +  +PK+A+GKILR++L   Y+++ AK
Sbjct:   27 SFFSKDDRFCVVPSAHNLDPVVGVTIPEFFWSNLHKWGNATAFECGVSGRKYTHDQVYKQSRSFAAALKQHGLKENDVVAILLPNVPEYAAILLGILEAGVTASPFNPTYTAHEIATQLNNCEAKAIVTLPMFLPQVQEAKAQC-----PLLKSVVVVGEAQPGCHSYFEMAKVNSDGVEFLKGSKIDTVNH-TAILPWSSGTTGPPKGVMLTHSNINTNLLQVT--QPGYLATKPFTGGNEQDKFIGILPFFHIYGFLLVMAKTLYHGAHTMTLPKFEPKSFIDSVRSHQPNFLHLVPPLISFMANSPDVKTEDLKSIACIMGGAAPIGPVIINRVLEKAGKHFLFTEGYGMTELSPVSHVSPVATNNKKIGSCGVPVPGSLCKVVDVETGEALGPNQPGELCIKGPQLMKGYYKNEKATNETIDAKGWLHTGDIGLYDEDKYFFIVDRLKELIKVRGFQVAPSELEDLLRKHPEVTDVAVIGVPDERAGELPRAYIVSKS-SKPSEESIHKYLETKVSPNKKLKGGVEFVQAIPKAASGKILRRELKKDYLSKHAK 575          

BLAST of probable 4-coumarate-- ligase 1-like vs. nr
Match: gi|340375302|ref|XP_003386175.1| (PREDICTED: probable 4-coumarate--CoA ligase 1 [Amphimedon queenslandica])

HSP 1 Score: 421.394 bits (1082), Expect = 9.404e-138
Identity = 222/541 (41.04%), Postives = 345/541 (63.77%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFHFMWENKDKYF-HKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVGEPNVVL------NVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPI--ATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDD-WLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDL 568
            IV   + D+PI + ++F  ++ N DK+     ALVDG++  +++Y+E    +   +  L+ LG  KGDV+ +  PN ++Y + F      G  ++T NP YT  E++ Q+  S + +  TT  LLPT+  AI   G+ +++   K+ ++ E          +M   ++ E +  L      N  ED+ VLPYSSGTTG+PKGVMLTH+NIV N+ Q T  H +I  +   + +      + +LP +H Y M V +  +L+ GGK V+LPKF+P++F+  +Q+Y+ +  +L PP++ F + HP V+ + L SI  +   AAP GP ++K+ K++    ++ R+ +G+TETSP+  +T+ S+  ++  S G    N   K+ DL+TG++LGP + GE+CI GP +MKGYL+ P+AT++ I DD W H+GDI  YD+ G F+I DRLKELIKVKG QV PAELE +++   KI D AVIGVPHE+ GEAPRA+VV ++ E++SEE++ ++V+ER++ +K L GGV+ +  VPKSA+GKILR++L
Sbjct:   43 IVTCPFPDIPIPETNVFSHVFRNFDKFTGSSPALVDGLSGAEVSYNEMLDVTSRTSSGLRRLGLQKGDVVTLCSPNSIDYGITFFATMAAGGVVSTCNPTYTASELSYQINNSGSNFVATTPSLLPTVLEAI---GNLNVK---KVLVINEDDNSNRETDNIMSLKVLKEDSGSLFKEEKVNAKEDLAVLPYSSGTTGLPKGVMLTHYNIVANLAQIT--HPEIMYLHEGEPN------LGLLPFFHIYGMVVILFHSLFSGGKCVVLPKFEPESFLSTIQKYRISTANLVPPIILFLSKHPLVEKYDLTSIRSVFSGAAPLGPDVLKEAKERTGIKII-RQGYGLTETSPVTHSTLISVGMDYPASIGVPIQNQSVKITDLSTGQSLGPQEEGEVCIAGPNVMKGYLNLPDATSKCITDDGWFHTGDIGYYDNNGFFYITDRLKELIKVKGLQVAPAELEGVLQHHPKIADAAVIGVPHERLGEAPRAFVVRRD-ESLSEEDVMSFVKERLSEHKWLTGGVQFIQEVPKSASGKILRRNL 567          

BLAST of probable 4-coumarate-- ligase 1-like vs. nr
Match: gi|1233200174|ref|XP_022199342.1| (4-coumarate--CoA ligase 1-like isoform X5 [Nilaparvata lugens])

HSP 1 Score: 422.55 bits (1085), Expect = 1.513e-137
Identity = 232/552 (42.03%), Postives = 337/552 (61.05%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKV---GHSDIRWKEKIFLVGEKVP---GYHSVSEMMDTDL--VGEPNVVLNVN---EDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMAEQAK 578
            IV S YEDV I D  L  F+WEN +K+  KIA+  G+T +   + +     K FA  L   G   G+V+A++LPN  +YP+   G    G+T++T+NP YTP EIA Q + SK+  AIT  + L  +  A A +   G S I        +G   P   G  S  EM++ +L   G  +V    N   +++  LPYSSGTTG+PKGV L+H N+V N+  Q +  ++   I   + +  Q     +LP +H Y   + M+ +L  GGKLV LP+F+P +FI+ L+  K + L++ PP+V F A HP +   H++ +  I   AAP+G S I++   K P + ++ +A+G+TETSP+  +   ++ +  + G  +  T AK++D +TG+ LGPN+ GELCI GPQ+M+GYL+N +AT E I + WLH+GDI  YDD+G F+IVDRLKELIKVKGFQV PAELE+L+R   K+ DVAVIGVP  + GE P AYV+ K+   V+E+E+  Y+ +R+A +K L  G+   D +PKS +GKILR+ L   Y+ E  K
Sbjct:   70 IVTSPYEDVQIPDALLHEFVWENYEKWADKIAIECGLTGRSYTFSKLRELCKKFAAALLKSGLKPGEVVAIILPNTPDYPIAALGAIEAGLTVSTVNPAYTPEEIAHQFRNSKSVCAITFTDKLDEVLKAKANLESDGGSPISVISINDFLGAAQPLPSGVWSFREMVENNLDTTGIESVCRKHNITQQNLVALPYSSGTTGLPKGVCLSHFNLVVNL--QQMAAKETEHILEPEGNVHQDVLPAILPFFHIYGFVIIMMSSLRHGGKLVTLPRFEPNSFIKVLETQKSSVLYIVPPIVLFLAAHPGITEQHVQHMRIITSGAAPAGASDIERCFQKMPKSFIFMQAYGLTETSPVCLLPRNNNRNLSTVGSPTSITKAKIIDTSTGKILGPNEHGELCIYGPQVMQGYLNNQKATDETIINGWLHTGDIGYYDDEGLFYIVDRLKELIKVKGFQVAPAELEELLRTHPKVNDVAVIGVPDARSGEVPLAYVIRKDD--VTEDELKGYISDRVAPFKQL-AGIVFTDSIPKSPSGKILRRFLKDAYLKEHKK 616          

BLAST of probable 4-coumarate-- ligase 1-like vs. nr
Match: gi|1207928921|ref|XP_021353674.1| (probable 4-coumarate--CoA ligase 1 [Mizuhopecten yessoensis])

HSP 1 Score: 416.387 bits (1069), Expect = 1.092e-135
Identity = 237/554 (42.78%), Postives = 327/554 (59.03%), Query Frame = 0

Query:   19 QIKLCLSARSLSISNGRFGIVRSQYEDVPISDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWK---EKIFLVGEKVPGYHSVSEMMDTDLVGEP-NVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDL 568
             I L    R  S S+ R  I  S   D+ I ++SL  ++    + + ++ ALVD    +   Y E           L  LG  KGDV+ +   N  EY L+       G+ +TT NP YT  E+A+ L  S     +  K LLPT++ AI     SDI+ K   + I +VGE + GY S S +++ D    P N+  N  +D+ +LPYSSGTTG+PKGVMLTH NIV N+ QQT         +P        T +C+LP++H Y M + +  TL  GGKLVIL +F+P  F++++Q+++ T L++ PP+  F A HP V  + + SI   + AAAP G SL ++++ +    VV  + +GMTE SP+AT+ +L   H G+ G L PN+ AK+V   TG  LGP + GELCI+GP +MKGYL+N  AT + I+D WL++GD+    D G F I DRLKELIK KGFQV PAELEDL+     +QD AVIG+P E+ GE PRAY+V    + V+ EEIS +V E  A +K L GGVE LD +PKS +GKILR+ L
Sbjct:   37 SITLQWCTRKFSSSSARNIIFTSPIPDIQIKEESLSEYLLPKFEHFGNRTALVDANNGKTYTYLEMRDAVIKLGSALHRLGYRKGDVLCMCSTNNTEYALLILACVTSGIILTTTNPAYTSGELARHLNHSGTSGVVVFKSLLPTVQEAI----ESDIKLKTTLKHIIVVGE-MDGYRSFSNLLNDDGTCFPDNITFNPKDDVALLPYSSGTTGLPKGVMLTHRNIVSNL-QQT---------RPQHIIKADDTILCLLPMFHGYGMIMILQNTLQDGGKLVILSQFEPVCFLKSIQQHRVTVLYMVPPMALFLARHPIVDTYDISSIRSHLSAAAPLGESLSREYEARLRYPVV--QGYGMTELSPVATLDTLPPHHIGTVGPLIPNSSAKIVCSETGADLGPGETGELCIRGPHVMKGYLNNEAATNDMIKDGWLYTGDVGHVRDDGCFVITDRLKELIKYKGFQVAPAELEDLLLRHPGVQDAAVIGMPDERAGEIPRAYIVPNPNKKVTSEEISKFVEENAASHKQLRGGVEFLDEIPKSPSGKILRRIL 573          

BLAST of probable 4-coumarate-- ligase 1-like vs. nr
Match: gi|1205902371|gb|OWF50401.1| (4-coumarate--CoA ligase 1 [Mizuhopecten yessoensis])

HSP 1 Score: 416.001 bits (1068), Expect = 1.218e-135
Identity = 237/554 (42.78%), Postives = 327/554 (59.03%), Query Frame = 0

Query:   19 QIKLCLSARSLSISNGRFGIVRSQYEDVPISDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWK---EKIFLVGEKVPGYHSVSEMMDTDLVGEP-NVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDL 568
             I L    R  S S+ R  I  S   D+ I ++SL  ++    + + ++ ALVD    +   Y E           L  LG  KGDV+ +   N  EY L+       G+ +TT NP YT  E+A+ L  S     +  K LLPT++ AI     SDI+ K   + I +VGE + GY S S +++ D    P N+  N  +D+ +LPYSSGTTG+PKGVMLTH NIV N+ QQT         +P        T +C+LP++H Y M + +  TL  GGKLVIL +F+P  F++++Q+++ T L++ PP+  F A HP V  + + SI   + AAAP G SL ++++ +    VV  + +GMTE SP+AT+ +L   H G+ G L PN+ AK+V   TG  LGP + GELCI+GP +MKGYL+N  AT + I+D WL++GD+    D G F I DRLKELIK KGFQV PAELEDL+     +QD AVIG+P E+ GE PRAY+V    + V+ EEIS +V E  A +K L GGVE LD +PKS +GKILR+ L
Sbjct:   36 SITLQWCTRKFSSSSARNIIFTSPIPDIQIKEESLSEYLLPKFEHFGNRTALVDANNGKTYTYLEMRDAVIKLGSALHRLGYRKGDVLCMCSTNNTEYALLILACVTSGIILTTTNPAYTSGELARHLNHSGTSGVVVFKSLLPTVQEAI----ESDIKLKTTLKHIIVVGE-MDGYRSFSNLLNDDGTCFPDNITFNPKDDVALLPYSSGTTGLPKGVMLTHRNIVSNL-QQT---------RPQHIIKADDTILCLLPMFHGYGMIMILQNTLQDGGKLVILSQFEPVCFLKSIQQHRVTVLYMVPPMALFLARHPIVDTYDISSIRSHLSAAAPLGESLSREYEARLRYPVV--QGYGMTELSPVATLDTLPPHHIGTVGPLIPNSSAKIVCSETGADLGPGETGELCIRGPHVMKGYLNNEAATNDMIKDGWLYTGDVGHVRDDGCFVITDRLKELIKYKGFQVAPAELEDLLLRHPGVQDAAVIGMPDERAGEIPRAYIVPNPNKKVTSEEISKFVEENAASHKQLRGGVEFLDEIPKSPSGKILRRIL 572          

BLAST of probable 4-coumarate-- ligase 1-like vs. nr
Match: gi|1233200170|ref|XP_022199340.1| (4-coumarate--CoA ligase 1-like isoform X3 [Nilaparvata lugens])

HSP 1 Score: 416.772 bits (1070), Expect = 2.775e-135
Identity = 230/552 (41.67%), Postives = 333/552 (60.33%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKV---GHSDIRWKEKIFLVGEKVP---GYHSVSEMMDTDL--VGEPNVVLNVN---EDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMAEQAK 578
            IV S YEDV I D  L  F+WEN +K+  KIA+  G+T +   + +     K FA  L   G   G+V+A++LPN  +YP+   G    G+T++T+NP YTP EIA Q + SK+  AIT  + L  +  A A +   G S I        +G   P   G  S  EM++ +L   G  +V    N   +++  LPYSSGTTG+PKGV L+H N+V N+  Q +  ++   I   + +  Q     +LP +H Y   + M+ +L  GGKLV LP+F+P +FI+ L+  K T +++ PP++ F   HP V    LE +  +   AAP G S + +   K P + ++ +A+G+TETSP+  +   ++ +  + G  +  T AK++D +TG+ LGPN+ GELCI GPQ+M+GYL+N +AT E I + WLH+GDI  YDD+G F+IVDRLKELIKVKGFQV PAELE+L+R   K+ DVAVIGVP  + GE P AYV+ K+   V+E+E+  Y+ +R+A +K L  G+   D +PKS +GKILR+ L   Y+ E  K
Sbjct:   70 IVTSPYEDVQIPDALLHEFVWENYEKWADKIAIECGLTGRSYTFSKLRELCKKFAAALLKSGLKPGEVVAIILPNTPDYPIAALGAIEAGLTVSTVNPAYTPEEIAHQFRNSKSVCAITFTDKLDEVLKAKANLESDGGSPISVISINDFLGAAQPLPSGVWSFREMVENNLDTTGIESVCRKHNITQQNLVALPYSSGTTGLPKGVCLSHFNLVVNL--QQMAAKETEHILEPEGNVHQDVLPAILPFFHIYGFVIIMMSSLRHGGKLVTLPRFEPNSFIKVLETQKSTIMYIVPPIMLFMTAHPAVTKKQLEHMRFLASGAAPIGASDVTRTLHKMPESCIFMQAYGLTETSPVCLLPRNNNRNLSTVGSPTSITKAKIIDTSTGKILGPNEHGELCIYGPQVMQGYLNNQKATDETIINGWLHTGDIGYYDDEGLFYIVDRLKELIKVKGFQVAPAELEELLRTHPKVNDVAVIGVPDARSGEVPLAYVIRKDD--VTEDELKGYISDRVAPFKQL-AGIVFTDSIPKSPSGKILRRFLKDAYLKEHKK 616          

BLAST of probable 4-coumarate-- ligase 1-like vs. nr
Match: gi|1233200166|ref|XP_022199338.1| (4-coumarate--CoA ligase 1-like isoform X2 [Nilaparvata lugens])

HSP 1 Score: 413.69 bits (1062), Expect = 3.569e-134
Identity = 231/553 (41.77%), Postives = 336/553 (60.76%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKV---GHSDIRWKEKIFLVGEKVP---GYHSVSEMMDTDL--VGEPNVVLNVN---EDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAP-SGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMAEQAK 578
            IV S YEDV I D  L  F+WEN +K+  KIA+  G+T +   + +     K FA  L   G   G+V+A++LPN  +YP+   G    G+T++T+NP YTP EIA Q + SK+  AIT  + L  +  A A +   G S I        +G   P   G  S  EM++ +L   G  +V    N   +++  LPYSSGTTG+PKGV L+H N+V N+  Q +  ++   I   + +  Q     +LP +H Y   + M+ +L  GGKLV LP+F+P +FI+ L+  K T L+L PP+V F A H ++   +++S+      AAP +   ++ KF  K P + ++ +A+G+TETSP+  +   ++ +  + G  +  T AK++D +TG+ LGPN+ GELCI GPQ+M+GYL+N +AT E I + WLH+GDI  YDD+G F+IVDRLKELIKVKGFQV PAELE+L+R   K+ DVAVIGVP  + GE P AYV+ K+   V+E+E+  Y+ +R+A +K L  G+   D +PKS +GKILR+ L   Y+ E  K
Sbjct:   70 IVTSPYEDVQIPDALLHEFVWENYEKWADKIAIECGLTGRSYTFSKLRELCKKFAAALLKSGLKPGEVVAIILPNTPDYPIAALGAIEAGLTVSTVNPAYTPEEIAHQFRNSKSVCAITFTDKLDEVLKAKANLESDGGSPISVISINDFLGAAQPLPSGVWSFREMVENNLDTTGIESVCRKHNITQQNLVALPYSSGTTGLPKGVCLSHFNLVVNL--QQMAAKETEHILEPEGNVHQDVLPAILPFFHIYGFVIIMMSSLRHGGKLVTLPRFEPNSFIKVLETQKCTILYLVPPMVHFVALHDEITVEYVDSVRFAFSGAAPLASCGILDKFVKKIPKSGLFMQAYGLTETSPVCLLPRNNNRNLSTVGSPTSITKAKIIDTSTGKILGPNEHGELCIYGPQVMQGYLNNQKATDETIINGWLHTGDIGYYDDEGLFYIVDRLKELIKVKGFQVAPAELEELLRTHPKVNDVAVIGVPDARSGEVPLAYVIRKDD--VTEDELKGYISDRVAPFKQL-AGIVFTDSIPKSPSGKILRRFLKDAYLKEHKK 617          

BLAST of probable 4-coumarate-- ligase 1-like vs. nr
Match: gi|1233200164|ref|XP_022199336.1| (4-coumarate--CoA ligase 1-like isoform X1 [Nilaparvata lugens] >gi|1233200168|ref|XP_022199339.1| 4-coumarate--CoA ligase 1-like isoform X1 [Nilaparvata lugens])

HSP 1 Score: 411.379 bits (1056), Expect = 3.255e-133
Identity = 232/553 (41.95%), Postives = 331/553 (59.86%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKV---GHSDIRWKEKIFLVGEKVP---GYHSVSEMMDTDL--VGEPNVVLNVN---EDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTLGHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAP-SGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHNGSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDDWLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMAEQAK 578
            IV S YEDV I D  L  F+WEN +K+  KIA+  G+T +   + +     K FA  L   G   G+V+A++LPN  +YP+   G    G+T++T+NP YTP EIA Q + SK+  AIT  + L  +  A A +   G S I        +G   P   G  S  EM++ +L   G  +V    N   +++  LPYSSGTTG+PKGV L+H N+V N+  Q +  ++   I   + +  Q     +LP +H Y   + M+ +L  GGKLV LP+F+P +FI+ L+  K T L L PP+  F A +  +  +HLE +      AAP S   +I  F  K P +  + +A+G+TETSP+  +   ++ +  + G  +  T AK++D +TG+ LGPN+ GELCI GPQ+M+GYL+N +AT E I + WLH+GDI  YDD+G F+IVDRLKELIKVKGFQV PAELE+L+R   K+ DVAVIGVP  + GE P AYV+ K+   V+E+E+  Y+ +R+A +K L  G+   D +PKS +GKILR+ L   Y+ E  K
Sbjct:   70 IVTSPYEDVQIPDALLHEFVWENYEKWADKIAIECGLTGRSYTFSKLRELCKKFAAALLKSGLKPGEVVAIILPNTPDYPIAALGAIEAGLTVSTVNPAYTPEEIAHQFRNSKSVCAITFTDKLDEVLKAKANLESDGGSPISVISINDFLGAAQPLPSGVWSFREMVENNLDTTGIESVCRKHNITQQNLVALPYSSGTTGLPKGVCLSHFNLVVNL--QQMAAKETEHILEPEGNVHQDVLPAILPFFHIYGFVIIMMSSLRHGGKLVTLPRFEPNSFIKVLETQKCTLLMLVPPIAQFIALNDKISVNHLERVRFSGSGAAPLSSSKIIDMFVSKIPKSAYFMQAYGLTETSPVCLLPRNNNRNLSTVGSPTSITKAKIIDTSTGKILGPNEHGELCIYGPQVMQGYLNNQKATDETIINGWLHTGDIGYYDDEGLFYIVDRLKELIKVKGFQVAPAELEELLRTHPKVNDVAVIGVPDARSGEVPLAYVIRKDD--VTEDELKGYISDRVAPFKQL-AGIVFTDSIPKSPSGKILRRFLKDAYLKEHKK 617          

BLAST of probable 4-coumarate-- ligase 1-like vs. nr
Match: gi|1061481214|gb|ODM98582.1| (4-coumarate--CoA ligase 2 [Orchesella cincta])

HSP 1 Score: 409.838 bits (1052), Expect = 3.894e-133
Identity = 225/545 (41.28%), Postives = 333/545 (61.10%), Query Frame = 0

Query:   38 IVRSQYEDVPISDKSLFHFMWENKDKYFHKIALVDGVTNQQINYDESYRRSKNFALRLQSLGGMKGDVMAVMLPNCVEYPLIFAGGPGVGVTITTLNPIYTPHEIAKQLQMSKACWAITTKELLPTLETAIAKVGHSDIRWKEKIFLVGEKVPGYHSVSEMMDTDLVG---EPNVVLNVNEDICVLPYSSGTTGVPKGVMLTHHNIVGNVCQQTL-GHRDISTIQPLQADTPQPTTICVLPLYHCYAMNVTMVPTLYCGGKLVILPKFDPKTFIQALQRYKPTFLHLAPPLVSFCATHPDVKPHHLESIEHIVVAAAPSGPSLIKKFKDKAPSNVVYREAWGMTETSPIATMTSLSDEHN--GSCGKLSPNTVAKVVDLNTGEALGPNQRGELCIKGPQIMKGYLDNPEATAENIQDD-WLHSGDIAIYDDQGRFFIVDRLKELIKVKGFQVPPAELEDLIRGFGKIQDVAVIGVPHEKFGEAPRAYVVAKEGETVSEEEISAYVRERMAVYKHLVGGVEILDHVPKSAAGKILRKDLFAKYMAE 575
            IV S     PI+  ++  F+W N  K+    A   GV+ ++  +    + S++F   L+  G  + DV+ ++LPN  EYP+   G     + ++ +NP+YTP EIAKQ+  S++   I   + +PT+   + K+  S     + I ++G+ V G HS SEM+  +  G         +  +++ +LPYSSGTTG PKGVML+H NI  N+ Q T  G+ + +     +    Q   I +LP +H +   V M  TLY G     LPKF+P TFI+AL+ +KP  +HL PPL++F A  PDV  +   SI  I+  AAP G +LI +  +KA     ++E +GMTE SP++ +     ++   GS G + P T  K+VD +TG ALG NQRGE+C+KGPQ MKGY  N +ATAE I  + WLH+GDI ++D+   F+IVDR+KELIKVKG QV P+ELED++R    + DVAVIG+P E+ GE PRAY+V K+ ET+SE E+  ++ E+++ +K L GGVE L  +PK+A+GKILR++L A Y+++
Sbjct:   46 IVTSNDHVDPITGVTIPEFIWSNLHKWEDAPAFECGVSGRKYTHGMINKLSRSFGAALKQHGLKRHDVVCLLLPNVPEYPIATLGAWEASLVVSPINPVYTPAEIAKQVNGSQSKVIIALSQFVPTVRE-VQKLSSS----LKSIIVIGDAVDGCHSFSEMVKVNSQGVEFARGSQFDTTDELALLPYSSGTTGPPKGVMLSHSNIASNLFQMTRPGYVETNF---FEKGDEQDRFIGILPFFHSFGFTVVMAKTLYQGAFTRTLPKFEPPTFIKALREHKPKCVHLVPPLIAFMANSPDVTKNEFVSIRAIMGGAAPIGEALITRLLEKAGKYFFFQEGYGMTEMSPVSHILPGYTKNTKIGSTGVILPGTQCKIVDPSTGAALGANQRGEICVKGPQNMKGYFKNDKATAETIDSEGWLHTGDIGLFDEGECFWIVDRMKELIKVKGLQVAPSELEDMLRTHPDVADVAVIGIPDERAGELPRAYIVRKK-ETLSENEVKEFIAEKVSDHKKLAGGVEFLPAIPKAASGKILRRELKALYLSK 581