hypothetical protein DAPPUDRAFT_308094, maker-scaffold421_size176100-snap-gene-0.22 (gene) Tigriopus kingsejongensis
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Overview
Associated RNAi Experiments
Nothing found Homology
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Match: EMLSAG00000004167 (supercontig:LSalAtl2s:LSalAtl2s220:203389:278765:1 gene:EMLSAG00000004167 transcript:EMLSAT00000004167 description:"maker-LSalAtl2s220-augustus-gene-2.5") HSP 1 Score: 393.66 bits (1010), Expect = 8.796e-121 Identity = 282/884 (31.90%), Postives = 423/884 (47.85%), Query Frame = 0
Query: 126 QQKVEITVKKYGKCETCQDKKCGPHEECIEDT-NGNPKCLCQG--CSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDP---CNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPV-DGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRN----PLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRSTFDMELKMTLISLMTDAPDQIYGQSKSPRQVLSDQKPSTGQFGETCL-TDIDCIVDNSHCSLNQCICDENHDYHADSRTCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVG-NSLMAVLAPADAVNRLEIKLKFRTF-AQNGLILYNEG-NLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVD-GDRVTGSSKGSLRTLNVGELIWIGNVDTQ---YEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRN-HCHPNPCKNKGQCQIARNENSFTCSCPVG-FFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIP--------VRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLP--ISDGNWHRV--NILRQDSSLIV 976
++ +E E C+ C CI D +G+ C C+G CS + ++ VCGSD ITY + C L +A CT+ ++ C+ C C FG C +N K Y + CH+E D C ++ + C C + C G LC C A CG + +S C + C KRG D + C ++ KYE+PIPCG+S C+FGA C C+C + C+ P YY AVCG DG TY +E LR+++C+ Q +I + +F PC T + G+ CL + DC+ NS C N+CIC E ++ + +C+ + + LCDPNPC GGGTC+EHDG FSCYC G G+ C+HDLS + ++ ASF G +S + + P D+V R+EI++ FRTF Q GL+LY + N+ E + P + + C+ + + F YDLGSG V L+S+ ++ G+W LVAKRYH+DGLLE++ D+V GS++GSLRTLN+ +WIG VD V K GT IGF GC+K+L++++ I+LQS +EP+V++R+ +++CEEN C PC NEG C + QGF C C +FTG+ CQR+RN C PNPC+N G C I + F C C ++G C+ ++ ++ ++K + + L +L K+ L ++ PG++G L + +++G L ++L +S+ + T+ P +SD H + NI R + L V
Sbjct: 181 RKLLETEASSGSNVEGCRHSNCRFGASCIVDAKSGDSYCDCKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVR---------------TGGNNNKIYKSQCHMELDAC----ESRLTNLTMSSHDNCYSCSQTCKWNEDCLYSEKSGKSLCS-CPECPSAGNEEEDPVCGTDNITYDSV-CSLRASTCSKKRG---------NDVRVKYPGVCRTI---KYEKPIPCGKSSCDFGAVCEEDS---YCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGAT--------------------------------------------RIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANTSCEIMK--------IGSLCDPNPCQGGGTCDEHDGTFSCYCRSGLTGSRCEHDLSLTDMNTASFTGQDSHVEIKIPTDSVKRMEIEIMFRTFNVQEGLLLYGQSVNVSNYENTIRTF--------PFFIPLSSSNNKCHVFKNIKHIIFSYDLGSGAVTLKSHGKIEKGKWTHLVAKRYHQDGLLELERTDKVIGSTRGSLRTLNLQGHLWIGGVDPNDFPLMYKVRKNAGTSIGFVGCIKELKINKKTISLQSMREPLVIRRRGLVECEENSCSGNPCKNEGLCISQVQGFLCHCKRDFTGKSCQRRRNDQCDPNPCQNNGVCHI--KDRGFVCVCSGSEYYGTYCDLKTADTPNDQPAYGSAKAPKTHFNNTLAFDLKKSLGLTEEALMELEVSFLKKDPGSLGRLKISSTKTGLLKLQLS---DSQGALTEWITSPPFFVSDNRPHNIILNIYRGELDLDV 963 HSP 2 Score: 130.954 bits (328), Expect = 3.884e-31 Identity = 110/372 (29.57%), Postives = 167/372 (44.89%), Query Frame = 0
Query: 19 CDVID-EARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKV-EITVKKYGKCETCQDKKCGPHEECI-EDTNGNPKCLCQGCSNKPSEE---VCGSDGITYFSLCHLQRASCTQNE--DIFAEHYGACD--------PCNEVTCKFGSYCSE-GQCICRQEC------PDSYE-PICASNGKTYFNYCHLEKDNCARQHDANFEQFR-------------VVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGV--CPCKRGFSGSKCE 351
C ID R N PVCGSDH+TY+N C+L +A C K+ N+T ISD CE+A+LC S CPF G C R G++N Y ++C + +C+ ++ +T+ + C +C + C +E+C+ + +G C C C + +EE VCG+D ITY S+C L+ ++C++ D+ ++ G C PC + +C FG+ C E C+C C P Y +C +G+TY L + C Q + F+ + E CL C + C CP G + + S C C G+ CD G C C+ G +GS+CE
Sbjct: 221 CKGIDCSGRINKPVCGSDHITYKNECDLLQAGCTKRINLTVISDLPCEEAHLCGSKGCPFGGSCVR----------------------TGGNNNKIYKSQCHMELDACESRLTNLTMSSHDNCYSCS-QTCKWNEDCLYSEKSGKSLCSCPECPSAGNEEEDPVCGTDNITYDSVCSLRASTCSKKRGNDVRVKYPGVCRTIKYEKPIPCGKSSCDFGAVCEEDSYCVCSVTCKEEEQFPKYYAGAVCGXDGRTYETEXDLRRHACKMQKEIVVADFKPCTRGATRIGDYCLHEESDCLALNSECVNNRCICPEGTSPNSANT-SCEIMKIGSL-CDPNPCQGGGT----CDEHDGTFSCYCRSGLTGSRCE 563
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Match: EMLSAG00000012366 (supercontig:LSalAtl2s:LSalAtl2s903:98980:175896:1 gene:EMLSAG00000012366 transcript:EMLSAT00000012366 description:"maker-LSalAtl2s903-augustus-gene-1.44") HSP 1 Score: 124.405 bits (311), Expect = 3.441e-29 Identity = 105/347 (30.26%), Postives = 155/347 (44.67%), Query Frame = 0
Query: 5 LSEDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSC-QQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQG-CSNKPSE--EVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSG 347
+S+ + C C C+ I P+CGSD +TY N C L+++AC K++ I+ I C CS C N +C + + F C+C C E EY PVCG++ Y N C+L + +C ++ +TV C C + C H C + +C C C N+ VCG+D ITY S C L+ AC C + C++G+ C +G C+C CP E +C ++ TY N C + K C +++ K+P+ + HG C++ S EC C+E GSE DCD Q G C CK G +G
Sbjct: 493 ISKQGITNCVCPQQCEPIIR-----PICGSDRITYENLCSLRKSACEKRKIISPIHYGICGSNGPCSKRKCNHNSICVESEDLAF-CECPSC--ESLEYHPVCGTNGKMYSNSCQLSREACLKEDDSLTVGDIKSCSGCANVTCDFHGYCHKG-----ECHCPSLCDNEIDHVGSVCGTDDITYPSECQLK--------------ITACHICRGINCQYGAKCLDGVCVCPTNCPRHEERLCGTDYMTYLNECEMLKATCTAKNEG---------------KIPISFLH-----HGP-------CRS------STKMTECHCSELGSEKLDCD-QYGKCSCKPGING 778 HSP 2 Score: 103.99 bits (258), Expect = 7.830e-23 Identity = 127/520 (24.42%), Postives = 198/520 (38.08%), Query Frame = 0
Query: 7 EDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEG--------------------------RFQCQC-------------QECY-----EEHREYEP--------------VCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCET----CQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYCSEGQCICRQECPD----SYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGC-----NEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTD----IAITNFAPCQ 448
ED C C++ C PVCGSD VTY N C++K A C K++ + ++ C N C CP C +Y+G R++ C QEC+ ++ +E P VC S+ Y N+C ++ SC++ +++ + C C+ C +EC G C+C +CGSD ITY +LC L++++C + + I HYG C PC++ C S C E + + ECP Y P+C +NGK Y N C L ++ C ++ D+ + V + C+ G CD F + G EC C NE C P + + C IC R I C +GA C + C+C +C + E +CGTD TY +EC++ + +C + + I+ + PC+
Sbjct: 289 EDSTIECQCKDTC-----PYAYTPVCGSDQVTYDNECKMKLAICRKKKRLKKVHSGIC-GVNSCLKLSCPDYSRCIPSYDGSSAKCVCPEPCKDSNNRSAIVCGLEVRYKGPCNPCSGFNCDVLRFQECHVLPIDQDKQERIPRCMCNTDCSDKTSPVCASNGQTYKNDCYMKLDSCRRLLDLHIVYPDTCSKGVNPCKLLNCDWGQECEISKQGITNCVCPQQCEPIIRPICGSDRITYENLCSLRKSACEKRKIISPIHYGICGSNGPCSKRKCNHNSICVESEDLAFCECPSCESLEYHPVCGTNGKMYSNSCQLSREACLKEDDS----------------LTVGDIKSCS---GCANVTCD-----FHGYCHKG--ECHCPSLCDNEIDHVGSVCGTDDITYPSECQLKITACHIC--------------------RGI-----NCQYGAKCLDG----VCVCPTNCPR---HEERLCGTDYMTYLNECEMLKATCTAKNEGKIPISFLHHGPCR 744 HSP 3 Score: 98.5969 bits (244), Expect = 3.060e-21 Identity = 112/441 (25.40%), Postives = 175/441 (39.68%), Query Frame = 0
Query: 44 ELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKC--ETCQDKKCGPHEECIEDTNGN-PKCLC-QGC--SNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCS-------------EGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRE---------CGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTD-IAITNFAPCQG--RITC 453
E ++ KQQ+ + C+D N PCPF GVC + +CQC++ Y PVCGSD V Y NEC+++ + C++K + G C +C C + CI +G+ KC+C + C SN S VCG + + G C+PC+ C + +C+C +C D P+CASNG+TY N C+++ D+C R D +V C + V C + C C+ + T+ C + CG + E+ C + C ++ S IC PC + +CN + C + C C C + Y+ VCGT+G Y + CQL + +C K+ D + + + C G +TC
Sbjct: 253 ESSSSSNAKQQHTRLQIAKSCDDEN-----PCPFGGVCVHMEDSTIECQCKDTCP--YAYTPVCGSDQVTYDNECKMKLAICRKKKRLKKVHSGICGVNSCLKLSCPDYSRCIPSYDGSSAKCVCPEPCKDSNNRSAIVCG----------------------LEVRYKGPCNPCSGFNCDVLRFQECHVLPIDQDKQERIPRCMCNTDCSDKTSPVCASNGQTYKNDCYMKLDSCRRLLD-----LHIVYPDTCSKGVNPCKLLNC--------DWGQECEISKQGITNCVCPQQCEPIIRPICGSDRITYENL-CSLRKSACEKRKIISPIHYGICGSNG-------------------PCSKRKCNHNSICVESEDLAFCECP-SCESLEYH--PVCGTNGKMYSNSCQLSREACLKEDDSLTVGDIKSCSGCANVTC 628
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Match: EMLSAG00000003993 (supercontig:LSalAtl2s:LSalAtl2s213:441137:447252:-1 gene:EMLSAG00000003993 transcript:EMLSAT00000003993 description:"maker-LSalAtl2s213-snap-gene-4.21") HSP 1 Score: 102.834 bits (255), Expect = 2.813e-22 Identity = 121/476 (25.42%), Postives = 183/476 (38.45%), Query Frame = 0
Query: 551 LCDPNPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLS-------------KSSISIAS-------------FVGN-SLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPV-------VLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRVTGSSKGSLRTLNVGELIWIG---NVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSV-----KNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFS---IPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGE 981
+C +PC GGTC F C C G G C D S K ++++ F G+ S M + I ++ R G I++ + DFLSL +++ V F + LG GP+ V V +W+ + A RY + G L DG TGS++ L TL+V + +++G N + D V K + GC+++L ++ L S + N+ DC+ C C N G CS+ ++G+EC CP + G C++ R N C+N G C I +E C CP+G+ G C D + E R+ Y +SF L S D S + V L+ G V LV L +E + S ++D WHRV I R L + +DG+
Sbjct: 989 ICSSSPCSNGGTCIGXGASFLCECTDGRYGITCSEDTSACTTGMHKCAVSSKCTVTLKGSYECICPFGKNPLFTGHGSYMQTHLKSAIRFNTHIHIEIRPQTLEGHIIHLGQSRNGRHQDFLSLLLVNGS-----------------------VVFSFSLG-GPIGGNVGNTVTIRTCCVEIDKWYSVEAGRYGRKGYLRXDGQFTTGSTESGLNTLDVDQELFLGGIPNSHMLFIDFVSKHY-----YEGCIRNLHINEKSYPLTSTGNH--WKGWNIKDCDGTACGGELCQNGGKCSLSENIHNHRGYECGCPDNYIGEHCEQHR--LCINACENDGICSIGDDE-IIHCDCPLGYHGSFCSDEE-EIRVPRF------YGDGFISFVLSETKSIRHDQTSDDYLGVGLKDGMV-----------HLVWNLGWFSRTELTVPS-----KVNDDKWHRVLIDRIRQELKLDVDGK 1407 HSP 2 Score: 85.5001 bits (210), Expect = 5.895e-17 Identity = 85/334 (25.45%), Postives = 143/334 (42.81%), Query Frame = 0
Query: 551 LCDPNPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNS-LMAVLAPADAVN--RLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPV-VLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGI---GFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQG--FECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSD 875
+C N C GGTC C CP + G C+ ++ I FVG+S L+ L+ + V ++L FRT + GLIL+ TGE D L + + ++ H F + R+ + G + L N + + + + + + GD+ S + +++++G V + K +G GI GF GC+K L+L+ + L + + +++++C+ C PC N C+ + C+CPP FTG C+R C NPC+ G C ++ + F C C G G LCE+ +
Sbjct: 468 ICTSNVCSNGGTCLTTLSGVKCICPLQYHGVQCEEEVF---IETPGFVGHSGLVHKLSKNNIVKDRNFHVQLSFRTSQEEGLILF------TGE-DVGYLLVSGYIKDGILTFKISCGHQI-----ILFSDPRHRVDXGYLQXLSINITI----FDTICSTSIQLNETRTMSGDQQIDSLP------QIPDMLYMGLVPHK------KELGNGIINVGFQGCMKHLQLNSNELNLYRD----AVDGRDIVECKSAICAIQPCRNGAICAQHEDSGSWFCDCPPGFTGALCERPV--CQTNPCQYGGTCLGSKTGSGFLCLCQAGRRGSLCEEGN 764 HSP 3 Score: 67.3958 bits (163), Expect = 1.756e-11 Identity = 85/355 (23.94%), Postives = 135/355 (38.03%), Query Frame = 0
Query: 551 LCDPNPCHGGGTC--EEHDGVFSCYCPPGFAGTLCQH---------------------DLSKSSISIASFVGNSLMAVLAPA---DAVNRLEIKLKFRT--FAQNGLILY-NEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQW-HELVAKRYHKDGLLEVDG-DRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKR-VGTGIGFTGCLKDLELSR------TLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHP--NPCKNKGQCQIARNENSFTCSCPVG 865
+C NPC GGTC + F C C G G+LC+ ++ + S S VG + P + + LE + F T Q L+ + + + +DF++L+ I + H + +DLG+GP + + V + H + R + G L+VD +TG S G LNV + + Y ++L GF+GC+ D+ T +AL S+ ++ +NV C + C PCSN G+C F CEC G C + C + C +C + + S+ C CP G
Sbjct: 726 VCQTNPCQYGGTCLGSKTGSGFLCLCQAGRRGSLCEEGNQIQVYIKRLNYKAYEYFLPEVELTQPSFRSSVGGYSSYLAYPVHTQNVLKSLEWQFHFSTPNTDQVALLFFLGQRGIHDFGSDFIALSYI-------------KGH----------ILMTWDLGAGPRRIFTKRPVDERYFVHAVRFGRNGRRGWLQVDNFPNITGLSPGKREFLNVTSMYYYIGGHENYNFSLLPHDFPVHRGFSGCMFDMAYRSYVTGQLTPLALPSKIGSSPIRGRNVEQCHYDICSSSPCSNGGTCIGXGASFLCECTDGRYGITCSEDTSACTTGMHKCAVSSKCTVTL-KGSYECICPFG 1056
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Match: EMLSAG00000000551 (supercontig:LSalAtl2s:LSalAtl2s108:383112:401265:1 gene:EMLSAG00000000551 transcript:EMLSAT00000000551 description:"maker-LSalAtl2s108-augustus-gene-4.10") HSP 1 Score: 100.523 bits (249), Expect = 1.269e-21 Identity = 93/287 (32.40%), Postives = 129/287 (44.95%), Query Frame = 0
Query: 30 PVCGSDHVTYRNGCELKEAAC-GKQQNITQISDQQCEDA------NLCSSDP---CPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECI----EDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNED-IFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKV---------PVCPVDGCT 292
PVCGSD TY N C+ + C + ++ +S C+D ++ DP CP C E+ PVCGSD V Y N+C L+ SC+ I + G+C+ P E I E+ + KC CS EEVCG+DG TY +LCHL+ SC ED I G C+ +++T + +C +CP+ Y PIC SNG TY N C L+ NC Q+D+ + ++ CL+K PVC DG T
Sbjct: 311 PVCGSDGQTYANKCQFESKNCLTSTKTLSIVSQGPCDDPIYEFEPSIAEEDPILECP--KFCTF------------------EFIPVCGSDGVTYSNKCHLQSQSCKSGNNIEMDHPGQCD-------NPFEAVITEEEEEEENDDKCPV-FCS-FTHEEVCGTDGTTYRNLCHLKYQSCQYPEDNIKKASNGPCNSKDKLT--------DDSNVCPVKCPNDYSPICGSNGLTYVNECQLKLANCNNQNDS---KIKISYYGSCLDKCPEFCATKYEPVCGSDGIT 557 HSP 2 Score: 88.5817 bits (218), Expect = 6.069e-18 Identity = 79/248 (31.85%), Postives = 107/248 (43.15%), Query Frame = 0
Query: 29 VPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNY-----EGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNED--IFAEHYGAC-DPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANF 268
+PVCGSD VTY N C L+ +C NI QC++ PF V + + C +EE VCG+D Y N C L+ SCQ E +KK + C ++ +D+N P C N S +CGS+G+TY + C L+ A+C D I +YG+C D C E C YEP+C S+G TY N CHL NC +HD +
Sbjct: 375 IPVCGSDGVTYSNKCHLQSQSCKSGNNIEMDHPGQCDN---------PFEAVITEEEEEEENDDKCPVFCSFTHEE------VCGTDGTTYRNLCHLKYQSCQYP-EDNIKK------ASNGPCNSKDKLTDDSNVCP----VKCPNDYSP-ICGSNGLTYVNECQLKLANCNNQNDSKIKISYYGSCLDKCPEF------------------CATKYEPVCGSDGITYVNDCHLLAGNC--RHDNKY 575 HSP 3 Score: 78.5666 bits (192), Expect = 6.237e-15 Identity = 82/292 (28.08%), Postives = 121/292 (41.44%), Query Frame = 0
Query: 31 VCGSDHVTYRNGCELKEAACG-KQQNITQISDQQC-------EDANLCSSD-PCPFNGVCARN---YEGRFQCQCQECYEEH---------------------REYEPVCGSDNVQYPNECELRKSSCQQKVE-ITVKKYGKCETCQDKKCGPHEECIEDT------NGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANF---EQFRVVESRVC 279
VCG+D TYRN C LK +C + NI + S+ C +D+N+C P ++ +C N Y Q + C ++ +YEPVCGSD + Y N+C L +C+ + I + G+C P + I DT N L Q N + EVCGSDG TY S+C + A+ + I+ + YG G+C C Q C + EP+C S+G Y N C + C+ D + F ++ R C
Sbjct: 442 VCGTDGTTYRNLCHLKYQSCQYPEDNIKKASNGPCNSKDKLTDDSNVCPVKCPNDYSPICGSNGLTYVNECQLKLANCNNQNDSKIKISYYGSCLDKCPEFCATKYEPVCGSDGITYVNDCHLLAGNCRHDNKYIFLLDLGEC--------SPKAQIISDTYEERSIRPNDNSLLQNTCN--TGEVCGSDGKTYSSVCEIIEANRNLSVKIY-KVYG------------------GKCTCPQVCSFNDEPVCGSDGIIYPNLCEFQIKMCSLFEDNKLGSEQLFLKIKGRQC 704 HSP 4 Score: 78.1814 bits (191), Expect = 8.757e-15 Identity = 72/244 (29.51%), Postives = 103/244 (42.21%), Query Frame = 0
Query: 31 VCGSDHVTYRNGCELKEAACGKQQNITQIS----DQQCEDANLCSSDP------CPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRK-SSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPS--EEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC-DPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNC 260
+CGS+ + Y N C + + +C + I QI D +D + S P + V + C R Y PVCGSD Y NEC L+ S C++K + G C+ ED + + C + P+ + VCG DG TY + C++ SCT+NE I H G C + N + S E C C Y+P+C S+G+TY N C E NC
Sbjct: 105 ICGSNGIVYVNTCYMMQESCRENVTIFQIPCSKIDPHYKDVLVPSRSPPHSSLVSSYLNVLPTSNTNPNSDNCLPDLTCSRIYYPVCGSDGNTYENECLLQGLSRCKRKNALVKAFTGDCDK-------------EDKDISESCNSFNATQCPTVYDPVCGIDGRTYTNECYMMATSCTENELIIKRHDGVCKNNSNLRKLRLASIIKE----CPFACNLIYQPVCGSDGQTYANKCQFESKNC 331 HSP 5 Score: 72.4034 bits (176), Expect = 5.402e-13 Identity = 76/297 (25.59%), Postives = 117/297 (39.39%), Query Frame = 0
Query: 30 PVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKC-----ETCQDKKCGPHEECIED-------TNGNP---KCLCQGCSNKPSEEVCGSDGITYFSLCHLQRAS-CTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCA------RQHD------ANFEQFRVVE-SRVC-----LEKVPVCPVDGCT 292
PVCGSDH TY N C L+ AC + + + GVC + C + + + +CGS+ + Y N C + + SC++ V I K + + PH + +N NP CL ++ VCGSDG TY + C LQ S C + + G CD ++ C+ +CP Y+P+C +G+TY N C++ +C ++HD +N + R+ + C L PVC DG T
Sbjct: 46 PVCGSDHTTYDNACRLQATACLDSKELR-----------------VEYEGVCHDRFPNEASCLLWKHCKNTSDSGKICGSNGIVYVNTCYMMQESCRENVTIFQIPCSKIDPHYKDVLVPSRSPPHSSLVSSYLNVLPTSNTNPNSDNCLPDLTCSRIYYPVCGSDGNTYENECLLQGLSRCKRKNALVKAFTGDCDKEDK---DISESCNSFNAT---QCPTVYDPVCGIDGRTYTNECYMMATSCTENELIIKRHDGVCKNNSNLRKLRLASIIKECPFACNLIYQPVCGSDGQT 319 HSP 6 Score: 58.9214 bits (141), Expect = 6.685e-9 Identity = 89/363 (24.52%), Postives = 140/363 (38.57%), Query Frame = 0
Query: 103 YEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHE-ECIEDTNGNPKCLCQGCSNKP-SEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQ-TGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESEC---NFGATCSNTGG----RLRCI---CEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTD-IAITNFAPCQGRI 451
+ PVCGSD+ Y N C L+ ++C E+ V+ G C D+ P+E C+ L + C N S ++CGS+GI Y + C++ + SC +N IF PC+++ + + SY + ++ + DNC + SR+ PVC DG T + RCK +F + DCD + + F+ ++C D D + ++ Y C E+E C N RL I C C N +Y + VCG+DG TY ++CQ +C T ++I + PC I
Sbjct: 44 WNPVCGSDHTTYDNACRLQATACLDSKELRVEYEG---VCHDR--FPNEASCL---------LWKHCKNTSDSGKICGSNGIVYVNTCYMMQESCRENVTIFQI------PCSKIDPHYKDVLVPSRSPPHSSLVSSYLNVLPTSNT------NPNSDNCLPD---------LTCSRI---YYPVCGSDGNTYENECLLQGLSRCKRKNALVKAF-------------TGDCDKEDKDISESCNSFNATQCPTVYDPVCGIDGR--TYTNECYMMATSCTENELIIKRHDGVCKNNSNLRKLRLASIIKECPFAC-NLIY--QPVCGSDGQTYANKCQFESKNCLTSTKTLSIVSQGPCDDPI 350
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Match: EMLSAG00000011190 (supercontig:LSalAtl2s:LSalAtl2s760:61742:91897:1 gene:EMLSAG00000011190 transcript:EMLSAT00000011190 description:"maker-LSalAtl2s760-augustus-gene-1.13") HSP 1 Score: 76.6406 bits (187), Expect = 1.180e-14 Identity = 71/245 (28.98%), Postives = 111/245 (45.31%), Query Frame = 0
Query: 16 ENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNC 260
E +C I + VCG+D VTY+N C L++A C I + + C + S+ CP + C + E R+ E CGSD Y +ECE++K++C KV + CE K C + +C D P+ + + VCGSD Y + C +++ +C ++ + P + F + G C + CPDSY+PIC ++ KTY N C +E +NC
Sbjct: 245 EAMCSKITCSNERDEVCGNDGVTYKNVCHLRKATC--YSGIEKSHNGACMSMSENRSENCPKS--------------CPKVANEDRKKELTCGSDGNIYKSECEMKKATCGMKV-VKTANLNSCEG--SKYC--NADC--DVGRKPRNM---------KMVCGSDSQFYRNECEMKKINCGKHMYVA--------PITQCLSNF-KFQFNG---CGRVCPDSYDPICGTDNKTYSNQCFMEMENC 445 HSP 2 Score: 57.3806 bits (137), Expect = 1.406e-8 Identity = 101/442 (22.85%), Postives = 162/442 (36.65%), Query Frame = 0
Query: 30 PVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHR---EYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQN-EDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCG------ESECNF-GATCS----NTGGRLRC----ICEIDC---RNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIA-----ITNF 444
PVCGSD V Y++ C ++ CG +I + ++C P C EH+ + VCG+D YPN C L+ +C++ +E+ + C+ T C + S +C S+G Y S+C ++R +C + +I H C+ +C G C C S+GK Y N C +++ NC +E V+ C K +C C+ +C + R+ C +S G C S ++ E CP C V++ ++ + CG +SEC ATC T C C DC R P + VCG+D YR+EC++++ +C K +A ++NF
Sbjct: 51 PVCGSDKVIYKSDCSMRVEKCG--GDINTLDLKECTK---------PNGATC-----------------EHKCDTTKDMVCGTDGRTYPNTCFLQVETCEKGIELAHLGH----------------CLNSTAVAEDCPSSCSKSDHSGPICASNGNVYPSICEMKRQTCGEGVVEISRSH-----------CESTRHCDSG-------CWRISRLTCGSDGKLYNNGCQMKRKNCGFPL-TRYENIVPVDREKCASKEAMCSKITCS---NERDEVCGNDGVTYKNVCHL--RKATCYSGIEKS-----HNGAC---MSMSENRSENCP-------KSCPKVANEDRKKELTCGSDGNIYKSECEMKKATCGMKVVKTANLNSCEGSKYCNADCDVGRKPRNMK-MVCGSDSQFYRNECEMKKINCGKHMYVAPITQCLSNF 408 HSP 3 Score: 54.299 bits (129), Expect = 1.072e-7 Identity = 38/127 (29.92%), Postives = 59/127 (46.46%), Query Frame = 0
Query: 15 CENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKK--YGKC 139
C CDV + R VCGSD YRN CE+K+ CGK + I+ C N++ +F + C + Y+P+CG+DN Y N+C + +C+ + V++ +GKC
Sbjct: 357 CNADCDVGRKPRNMKMVCGSDSQFYRNECEMKKINCGKHMYVAPITQ-------------------CLSNFKFQFNGCGRVCPD---SYDPICGTDNKTYSNQCFMEMENCRARSLGGVRRKYFGKC 461
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Match: EMLSAG00000006314 (supercontig:LSalAtl2s:LSalAtl2s343:642:15163:-1 gene:EMLSAG00000006314 transcript:EMLSAT00000006314 description:"maker-LSalAtl2s343-augustus-gene-0.12") HSP 1 Score: 72.0182 bits (175), Expect = 8.291e-13 Identity = 35/103 (33.98%), Postives = 47/103 (45.63%), Query Frame = 0
Query: 798 NPCVRMPCSNEGSCSVKN-----------------------QGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPE 877
+PC PC + G CS + Q C C PE+ G RCQ ++N C+PNPC G C + R+E+SF C CP G LCE + +
Sbjct: 3662 DPCSNNPCLHFGQCSSRKIFVNHSTSIVESSDTIFNSPLLLQDVRCICTPEYAGSRCQIQKNPCNPNPCLEGGNC-VVRSESSFQCLCPPLRGGDLCETTQTD 3763 HSP 2 Score: 67.781 bits (164), Expect = 1.562e-11 Identity = 83/372 (22.31%), Postives = 139/372 (37.37%), Query Frame = 0
Query: 508 CIVDNSHCSLNQCICDENHDYHADSR---TCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEE--HDGVFSCYCPPGFAGTLCQHDLS--------KSSISIA-----------SFVGNSL-----------MAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEV---------------DGDRVTGSSKGSLRTLNVG-ELIWIGNVDTQYEDNVLKRVG--TGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVR-MPCSNEGSCSVKNQGFECECPPE 825
C + + C+ N C+ N ++S C L D + +C PNPC GG+C++ D F C C PG+ G C+ + + I+ +F GN A P D+ N +I + F T N L++YN G + G +DFL+L ++ P F + R + +L ++ G+W+++ A R ++ L + D R G TL G +++G + Q E L+R G + F GC+K L ++ Q ++ + R + +NPC + C G+C +C C E
Sbjct: 3718 CQIQKNPCNPNPCLEGGNCVVRSESSFQCLCPPLRGGDLCETTQTDVCSPNPCFNGGSCQKGXRDSFF-CRCRPGYQGDRCERAVDSCLRNPCLNGGVCISQKPDYRCRCPDNFYGNRCELSTFGFDELSFAAFPPLDS-NTNDISITFATTKANSLLVYNHGEVSGGRSDFLALELVR------------------GRPKFSWGAAR----TAVTILELPRKINNGRWYKVTATRNNRVASLSIEDCTESAEYCKLCQQDDSRCFSKDLGEAGTLVFGNNPMYLGGL--QSEIPFLERPGQLSSDDFVGCIKTLTINGNEKDFQ--KDALNTSRISSTCNFDNPCHKGNECGETGTCLPLWSRHQCSCGTE 4061 HSP 3 Score: 67.781 bits (164), Expect = 1.714e-11 Identity = 32/79 (40.51%), Postives = 43/79 (54.43%), Query Frame = 0
Query: 796 EENPCVRMPCSNEGSCSVKNQ-GFECECPPEFTGRRCQRKRNH-CHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCE 872
++NPC PC G+C V+++ F+C CPP G C+ + C PNPC N G CQ +SF C C G+ G CE
Sbjct: 3721 QKNPCNPNPCLEGGNCVVRSESSFQCLCPPLRGGDLCETTQTDVCSPNPCFNGGSCQKG-XRDSFFCRCRPGYQGDRCE 3798 HSP 4 Score: 63.929 bits (154), Expect = 2.548e-10 Identity = 51/183 (27.87%), Postives = 72/183 (39.34%), Query Frame = 0
Query: 800 CVRMPCSNEGSCSVKNQ-GFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGN----VGHLALKVSQSGQLVMEL---RETLE---SEPVIASLETNLPISDGNWHRVNILRQD 971
C PC N GSC + F C C P + G RC+R + C NPC N G C + + C CP F+G CE ST +D ++SF L + SI N H + +S L +EL R + + LE I++G W++V R +
Sbjct: 3765 CSPNPCFNGGSCQKGXRDSFFCRCRPGYQGDRCERAVDSCLRNPCLNGGVC--ISQKPDYRCRCPDNFYGNRCE------------LSTFGFD--ELSFAAFPPLDSNTNDISITFATTKANSLLVYNHGEVSGGRSDFLALELVRGRPKFSWGAARTAVTILELPRKINNGRWYKVTATRNN 3931
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Match: EMLSAG00000007498 (supercontig:LSalAtl2s:LSalAtl2s430:288057:377591:1 gene:EMLSAG00000007498 transcript:EMLSAT00000007498 description:"maker-LSalAtl2s430-augustus-gene-4.9") HSP 1 Score: 70.8626 bits (172), Expect = 1.164e-12 Identity = 81/304 (26.64%), Postives = 128/304 (42.11%), Query Frame = 0
Query: 556 PCHGGGTC----EEHDGVFSCYCPPGFAGTLCQHDLSKSSIS--IASFVGNSLMAVLAPADAVNRL-EIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEV------DGDRVTGSSK---GSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEE----------NPCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQR 833
PC G C H+ ++ C GTL L K IS + SF G S + D+ EI+L F++ A++GLI YN G G DF+++ ++ R +VEF YDLG G V+RS + + W+ +V R + +EV D ++ SS+ G+ L + + +++G V T D V + F GC++ L ++ I L + + +V C+ N + PC G+C GF+C+CP TG CQ+
Sbjct: 313 PCQHNGKCVPLESGHNIWWAINC-----GTLSI-SLRKGIISKNVPSFNGTSYLVYTGIGDSSPLWTEIELVFQSSAEDGLIFYN-GQRNDGTDDFIAI-LLSR----------------------NYVEFAYDLGDGITVVRSASPISLHVWYTVVISRTGRLAEMEVIRGKREDPLKMPVSSRVSNGAFSQLTLEQNLYLGGVPTF--DLVSAYLPIRRSFFGCIQKLTINDVEINLVNS----AISGVDVGSCDHPCNTPSSSSSNEFPQHPCGVHGNCKPVLDGFKCDCPHGLTGNSCQK 580
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Match: EMLSAG00000000936 (supercontig:LSalAtl2s:LSalAtl2s1165:50208:68601:-1 gene:EMLSAG00000000936 transcript:EMLSAT00000000936 description:"maker-LSalAtl2s1165-augustus-gene-0.27") HSP 1 Score: 70.0922 bits (170), Expect = 2.809e-12 Identity = 86/330 (26.06%), Postives = 128/330 (38.79%), Query Frame = 0
Query: 571 SCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLI--LYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPV----VLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRVTGSSK----------GSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQ-----SEQEPMVMQRKNVIDC-EENPCVRMPCSNEGSCSVKNQGFECECP------PEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCE 872
+C C PG+ G C H+ S+ S+V +L P EI+L+FRT ++G + + ++ N E G I++ E + FRY+L S + S+ V GQWH + RY +L +DG G S+ L T++ E ++ G +Y V + GC+ D+ L + L ++ M R +C NPC + C C +EC CP P+ G+ C RN C NPC+N GQC + C CP GF G CE
Sbjct: 2503 TCKCNPGWTGQFCSHETMPSTFDPQSYVKYALS--FEPDSFAT--EIQLRFRTREEHGELFRVSDQHNREYG--------ILEIQESKL--------------------RFRYNLNSLRTEEKEIWLSSIYVDDGQWHTVKVARYGSAAILTLDG----GESRRYNDSFIFDGHQLMTVDKQEGVYAGG-KAEYTGVRTFEVYSDYA-KGCMDDIRLEGKXLPLPPALNGTQWGQATMARNLQRNCPSNNPCANVICPKPFVCVDLWMEYECSCPRGQRVTPD--GKSCV-DRNECLDNPCQNNGQCVNRDTAERYICHCPSGFSGSNCE 2791
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Match: EMLSAG00000003115 (supercontig:LSalAtl2s:LSalAtl2s174:638829:676917:-1 gene:EMLSAG00000003115 transcript:EMLSAT00000003115 description:"maker-LSalAtl2s174-augustus-gene-6.13") HSP 1 Score: 69.3218 bits (168), Expect = 3.561e-12 Identity = 67/227 (29.52%), Postives = 99/227 (43.61%), Query Frame = 0
Query: 617 IKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLR-SNAQVVPGQWHELVAKRYHKDGLLEVDGDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLK------RVGTGIGFTGCLKDLELSRTLIALQS--EQEPMVMQRKNVIDCEENP--CVRMPCSNEGSCSVKNQGFECECP-PEFTGRRC 831
I LKFRT NG++L+N GN D+ + I+D H H DLGSGPV +R S + G WH + + G +++D D + + G L++ +++G VD Y D +K V GF GCL+DL ++ I + S Q+ + R + C + P C PC++ G CS F C+C FTG C
Sbjct: 509 ISLKFRTNELNGILLFNGGN---PHKDYFAFEILD-------------GHLYVH----------LDLGSGPVKIRASRTPLNDGAWHHVELTLSQRSGRIKIDEDSESFKTPGEANQLDLVGNLFVGGVD--YMDPYMKFPPTLWTVTLKYGFVGCLRDLTINGASIDVVSYAHQQDVGSIRSS---CHKMPDQCHDRPCNHGGKCSEGWNRFLCDCSRTSFTGATC 704
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Match: EMLSAG00000003184 (supercontig:LSalAtl2s:LSalAtl2s176:575372:647076:1 gene:EMLSAG00000003184 transcript:EMLSAT00000003184 description:"augustus_masked-LSalAtl2s176-processed-gene-6.1") HSP 1 Score: 68.1662 bits (165), Expect = 9.264e-12 Identity = 86/355 (24.23%), Postives = 130/355 (36.62%), Query Frame = 0
Query: 557 CHGGGTCEEHDGVFSCYCPPGFAGTLC-----------------QHDLSKS-------------------------SISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFR-FVEFRYDLGSGPV-VLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRVTG-SSKGSLRTLNVGELIWIGNVDTQYEDNVLKR--VGTGIGFTGCLKDL----ELSRTLIALQSEQEPM----VMQRKNVIDCEENPCVRMPCSNEGSCSVKNQG--FECECPPEFTGRRCQ----RKRNHCHPNPCKNKGQCQ 850
C GGTCE+ ++C CP F G C QHD ++ SF+ N+ + + + I + F T QNG++LY TG+ L++ + FR + YD+G+ PV + S + G++H + K+ L VDG ++G+ L + ++I V LK+ + F GC+K++ +LS L A + + + Q + D PCV C +EG C KN G +EC C P FTG CQ KR H P K Q Q
Sbjct: 454 CQNGGTCEDGIDSYTCKCPQEFGGKFCEIEPMVAQLYPQTSPCQQHDCKHGICFQPPGSNNYICKCAPGYSGKRCEYLTSLSFIHNTSYVEMEALNVKPKANITIIFATEQQNGVLLY------TGDNQHLAVEL------------------------FRGRIRVSYDVGNYPVSTMFSYELLSDGKYHRVELLAIKKNFTLRVDGGIARSIVNEGNNEYLEIRNSLFIAGVPEDVGHKSLKQWHLRNSTSFKGCIKEVYVNDKLSDFLQAAKVRHKVTPGCSLYQDEQPTD----PCVDHKCKHEGECRSKNDGTSYECICKPHFTGHFCQIRGKSKRQHPTVVPGIGKRQQQ 774 HSP 2 Score: 53.1434 bits (126), Expect = 3.761e-7 Identity = 47/194 (24.23%), Postives = 87/194 (44.85%), Query Frame = 0
Query: 800 CVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQ---------RKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIP----VRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDG 980
C+ C N G+C + C+CP EF G+ C+ + + C + CK+ G C N++ C C G+ G+ CE S + +TS + + ++ + N++ +F+ V L G+ HLA+++ + + + + + PV L +SDG +HRV +L + + +DG
Sbjct: 449 CLNHMCQNGGTCEDGIDSYTCKCPQEFGGKFCEIEPMVAQLYPQTSPCQQHDCKH-GICFQPPGSNNYICKCAPGYSGKRCEYLTSLS----FIHNTSYVEMEALNVKPKANITI---IFATEQQNGVLLYTGDNQHLAVELFRGR---IRVSYDVGNYPVSTMFSYEL-LSDGKYHRVELLAIKKNFTLRVDG 630
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Match: gi|218563482|sp|A2ASQ1.1|AGRIN_MOUSE (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22) HSP 1 Score: 198.364 bits (503), Expect = 2.902e-50 Identity = 153/490 (31.22%), Postives = 215/490 (43.88%), Query Frame = 0
Query: 5 LSEDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCE---------------TCQDKKCGPHEECIEDTNGNPKCLCQ-GCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVC--------------PVDG-CTCPHGVGGHLCDRCKTAFWAFTSF------GCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICE-IDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRS 456
L E + C C + C ++ A+ PVCGSD TY + CEL AC Q ++ S C+ C C F VC+ QC C C EH PVCGSD V Y + CELR+++CQQ+V+I + G CE C+ + C H ++ + + C+C C + VCGSDG+TY + CHL++A C ++++ GAC G T + +CA+ + V L P PV G C+C GVGG CDRC+ FW F GC C C+ G+ DC+ TG+C C+ G +G KC CPDG GC + +T P+ C E C FGA+C G +C+C + C P VCG+DG TY +ECQL+ +CR++ DI+I + PC+ + G S
Sbjct: 440 LCEVETGRCVCPSEC--VESAQ---PVCGSDGHTYASECELHVHACTHQISLYVASAGHCQT---CGETVCTFGAVCSAG-----QCVCPRC--EHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCEPAECGSGGSGSGEDNACEQELCRQHGGVWDEDSEDGPCVCDFSCQSVLKSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGAC-----------------------------------RGPTLAPLLPMASPHCAQTPYGCCQDNVTAAQGVGLAGCPSTCHCNPHGSYSGTCDPVTGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQALGHLGCEADPTT----PVTCVEMHCEFGASCVEEAGFAQCVCPTLTC--PEANSTKVCGSDGVTYGNECQLKTIACRQRLDISIQSLGPCRESVAPGVS 873 HSP 2 Score: 176.022 bits (445), Expect = 2.231e-43 Identity = 133/434 (30.65%), Postives = 193/434 (44.47%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEEHDGV--FSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQG-FECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIP-VRLRPGNVGHL---ALKVSQSGQLV--------MELRETLESEPVIASLETNLPISDGNWHRVNILR 969
CD PC GGTC++ D FSC C G AGT+C+ S+ +F G+S +A A + L + L+FR GL+LYN GN DFL+L ++D V+FR+D GSGP VL S V PG+WH L R+ + G L VDG+ V G S LN+ +++G + + VL R GIG GC++ L+++ + L S+ + V+Q V +C ++PC PC C G F C+CPP G C ++N C PNPC C + + C+CP+G G CE + + + + ++ GL T + ++ V L G G L K G V +E R L I + + PI+ G W RV + R
Sbjct: 1215 CDSQPCLHGGTCQDLDSGKGFSCSCTAGRAGTVCE---KVQLPSVPAFKGHSFLA-FPTLRAYHTLRLALEFRALETEGLLLYN-GNARG--KDFLALALLDG-----------------------HVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGEAPVVGESPSGTDGLNLDTKLYVGGLPEEQVATVLDRTSVGIGLKGCIRMLDINNQQLEL-SDWQRAVVQSSGVGECGDHPCSPNPCHGGALCQALEAGVFLCQCPPGRFGPTCADEKNPCQPNPCHGSAPCHVL-SRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELK-GLHTFERDLGEKMALEMVFLARGPSGLLLYNGQKTDGKGDFVSLALHNRHLEFRYDLGKGAAI--IRSKEPIALGTWVRVFLER 1613 HSP 3 Score: 144.05 bits (362), Expect = 1.716e-33 Identity = 123/436 (28.21%), Postives = 186/436 (42.66%), Query Frame = 0
Query: 28 NVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCE----TCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCS--EGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKV----PVCPVDGCTCP---HGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESEC-NFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQG 449
+ P+CG D VTY N C + + + ++ QC+ + C + C FN VC + GR C C + Y PVC D Y N+C +++ C+Q+ I K G C+ C+ +C C NG C CQ + + VCGSDG+TY S+C L+ +CT +I G CD C + C+FGS C G+C+C EC +S +P+C S+G TY + C L C H + + C E V VC C CP H G +C + + RE C + + + +A+ G C + C S S E C + C G C+C+ C++ L + VCG+DG TY +EC L++ C + ++ + C+G
Sbjct: 248 HTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCP-ETCQFNSVCL-SRRGRPHCSCDRVTCDG-AYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVK-NGKAVCECQRVCSGGYDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQ--CRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACT--HQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACEL--REAACQQ---QVQIEEARAGPC--------------------EPAECGSGGSGSGEDNA-CEQELCRQHGGVWDEDSEDGPCVCDFSCQSVL--KSPVCGSDGVTYSTECHLKKARCEARQELYVAAQGACRG 647 HSP 4 Score: 130.568 bits (327), Expect = 2.475e-29 Identity = 141/478 (29.50%), Postives = 197/478 (41.21%), Query Frame = 0
Query: 29 VPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGR-FQCQC-QECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEEC-IEDTNGNPKCLCQGCSNKPSEE--VCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYC----SEGQCIC-RQECPDSYEPICASNGKTYFNYCHLEKDNCARQH----------DANFEQFRVVE------------------SRVCLEKV-PVCPVDGCTCPHGVGGHLCDRCKTA------FWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGC----------ISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQ 448
PVCGSD TY N CEL+ A C +Q+ I + C + C++ C F C + +G+ C C C+ VCGSD V YP+EC+L + +C + I K G C+ CQ + C + +P+ L + N P++ +CG DG+TY + C + R + + G C D C E TC+F S C C C R C +Y P+CA +G TY N C ++ C +Q D R + RVC PVC DG T G +C+ A C CG FGS C+ +TG C C S +C + S C IS+ CGE+ C FGA CS +C+C C +P VCG+DG TY S C+LR+ +C++Q I PC+
Sbjct: 102 APVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGT--VCGSDGVDYPSECQLLRHACANQEHIFKKFDGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRP-ENCPAQHTPICGDDGVTYENDCVMSRIGAARGLLLQKVRSGQCQTRDQCPE-TCQFNSVCLSRRGRPHCSCDRVTCDGAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVKNGKAVCECQRVCSGGYDPVCGSDGVTY-----GSVCELESMACTLGREIRVARRGPCDRCGQCRFGSL---CEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAG----QCVCP-RCEHP--PPGPVCGSDGVTYLSACELREAACQQQVQIEEARAGPCE 560 HSP 5 Score: 127.872 bits (320), Expect = 2.027e-28 Identity = 92/262 (35.11%), Postives = 124/262 (47.33%), Query Frame = 0
Query: 5 LSEDQVPFCACENL-CDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDA-NLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCIC-RQECPDSYEPICASNGKTYFNYCHLEKDNCARQ 263
LS P C+C+ + CD PVC D TY N C ++A C +QQ I C+ + C C F C G+ C+CQ Y+PVCGSD V Y + CEL +C EI V + G C+ C + G C +T +C+C + ++ VCGSDG TY S C L +CT ++ G C C E C FG+ CS GQC+C R E P P+C S+G TY + C L + C +Q
Sbjct: 301 LSRRGRPHCSCDRVTCD-----GAYRPVCAQDGHTYDNDCWRQQAECRQQQTIPPKHQGPCDQTPSPCRGAQCAFGATCTVK-NGKAVCECQRVCSGG--YDPVCGSDGVTYGSVCELESMACTLGREIRVARRGPCDRCGQCRFG--SLCEVETG---RCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGETVCTFGAVCSAGQCVCPRCEHPPP-GPVCGSDGVTYLSACELREAACQQQ 548 HSP 6 Score: 113.235 bits (282), Expect = 5.757e-24 Identity = 111/441 (25.17%), Postives = 180/441 (40.82%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEEHD-GVFSCYCPPGFAGTLCQHDL--SKSSISIASFVGNSLMAVLA----PADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEV-DGDRVTGSSKGSLRT----LNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRM---PCSNEGSCSVKNQGFECECPPEFTGRRCQR---KRNHCHPNPCKNKGQCQI----ARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQ 970
C PNPCHG C G C CP G +G+ C+ L + S +A F G S + + D ++ +++ F +GL+LYN G G+ DF+SL + + R +EFRYDLG G ++RS + G W + +R + G L+V DG RV G S S + LN+ E +++G + + GF G ++ + L + Q V++ +V +PC + PC N GSC + +EC CP F+G C++ +++ G+ I A E+ T P PE+ R S L+T ++ L+ V R ++AL + ++L L S+PV+ L + + ++ W RV R+
Sbjct: 1474 CQPNPCHGSAPCHVLSRGGAKCACPLGRSGSFCETVLENAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYN-GQKTDGKGDFVSLAL-----------------------HNRHLEFRYDLGKGAAIIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYVGGAPD--FSKLARGAAVASGFDGAIQLVSLRGHQLLTQEH----VLRAVDVAPFAGHPCTQAVDNPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPA-----------PETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKVGER---ADYMALAIVDGH---LQLSYDLGSQPVV--LRSTVKVNTNRWLRVRAHRE 1865 HSP 7 Score: 91.2781 bits (225), Expect = 3.177e-17 Identity = 69/252 (27.38%), Postives = 107/252 (42.46%), Query Frame = 0
Query: 555 NPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSIS---IASFVGNSLMAVL-----------APA------------DAVNRLEIKLKFRTFAQNGLILYNEGNLETGE-TDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIAL 778
NPC GG+C + + C CP GF+G C+ + + S+ +F G + + L PA A+ +L RT A GL+L+ + GE D+++L I+D ++ YDLGS PVVLRS +V +W + A R H++G L+V + VTGSS L+ +W+G + L + G GF GCL+D+ + + L
Sbjct: 1710 NPCLNGGSCIPREATYECLCPGGFSGLHCEKGIVEKSVGDLETLAFDGRTYIEYLNAVTESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIG---KVGERADYMALAIVD-----------------------GHLQLSYDLGSQPVVLRSTVKVNTNRWLRVRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKA-YGTGFVGCLRDVVVGHRQLHL 1934
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Match: gi|399021|sp|P25304.2|AGRIN_RAT (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22) HSP 1 Score: 195.667 bits (496), Expect = 1.635e-49 Identity = 154/486 (31.69%), Postives = 212/486 (43.62%), Query Frame = 0
Query: 5 LSEDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCET---------------CQDKKCGPHEECIEDTNGNPKCLCQ-GCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCP-----------VDGCTCPHGVGGHLCDRCKTAFWAFTSF------GCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICE-IDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRS 456
L E + C C + C ++ A+ PVCGSD TY + CEL AC Q ++ S C+ C C F VC+ QC C C EH PVCGSD V Y + CELR+++CQQ+V+I G CE C+ + C ++ + + C+C C + P VCGSDG+TY + C L++A C ++++ GAC P A F +C C + NF + V C P C+C GVGG CDRC+ FW F GC C C+ G+ DC+ TG+C C+ G +G KC CPDG + GC + T P+ C E C FGA+C G +CIC + C P VCG+DG TY +ECQL+ +CR++ DI+ + PCQ +T G S
Sbjct: 440 LCEVETGRCVCPSEC--VESAQ---PVCGSDGHTYASECELHVHACTHQISLYVASAGHCQT---CGEKVCTFGAVCSAG-----QCVCPRC--EHPPPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCEPAECGSGGSGSGEDDECEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGAC----------------------------RGPTLAPLLPVAFPHCAQTPYGCCQD---NFTAAQGVGLAGCPSTCHCNPHGSYSGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGHSGCTPCSCDPRGAVRDDCEQMTGLCSCRPGVAGPKCGQCPDGQVLGHLGCEADPMT----PVTCVEIHCEFGASCVEKAGFAQCICPTLTC--PEANSTKVCGSDGVTYGNECQLKAIACRQRLDISTQSLGPCQESVTPGAS 873 HSP 2 Score: 165.236 bits (417), Expect = 4.931e-40 Identity = 105/325 (32.31%), Postives = 155/325 (47.69%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEEHDG--VFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQG-FECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCE 872
CD PC GGTC++ D F+C C G G++C+ S+ +F G+S +A A + L + L+FR GL+LYN GN DFL+L ++D V+FR+D GSGP VL S V PG+WH L R+ + G L VDG+ V G S LN+ +++G + + VL R G+G GC++ L+++ + L Q V Q V +C ++PC+ PC C G F C+CPP G C +++ C PNPC C++ + + C CP+G G C+
Sbjct: 1224 CDSQPCLHGGTCQDQDSGKGFTCSCTAGRGGSVCE---KVQPPSMPAFKGHSFLA-FPTLRAYHTLRLALEFRALETEGLLLYN-GNARG--KDFLALALLD-----------------------GRVQFRFDTGSGPAVLTSLVPVEPGRWHRLELSRHWRQGTLSVDGETPVVGESPSGTDGLNLDTNLYVGGIPEEQVAMVLDRTSVGVGLKGCIRMLDINNQQLELSDWQRAAV-QSSGVGECGDHPCLPNPCHGGALCQALEAGMFLCQCPPGRFGPTCADEKSPCQPNPCHGAAPCRVLSSGGA-KCECPLGRSGTFCQ 1516 HSP 3 Score: 149.443 bits (376), Expect = 4.514e-35 Identity = 128/442 (28.96%), Postives = 188/442 (42.53%), Query Frame = 0
Query: 28 NVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCE----TCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCS--EGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKV----PVCPVDGCTCP---HGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRL-------RCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQG 449
+ P+CG D VTY N C + + + ++ QC+ + C + C FN VC + GR C C + Y PVC D Y N+C +++ C+Q+ I K G C+ C +C C NG +C CQ + + VCGSDG+TY S+C L+ +CT +I G CDPC + C+FGS C G+C+C EC +S +P+C S+G TY + C L C H + + C EKV VC C CP H G +C + + RE C + + + +A G C + C S S E + EC C GG C+C+ C++ R VCG+DG TY +EC L++ C Q ++ + C+G
Sbjct: 248 HTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCP-ETCQFNSVCL-SRRGRPHCSCDRVTCDG-SYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVK-NGKAECECQRVCSGIYDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQ--CRFGSLCEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACT--HQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPPGPVCGSDGVTYLSACEL--REAACQQ---QVQIEEAHAGPC--------------------EPAECGSGGSGSGE------DDECE-QELCRQRGGIWDEDSEDGPCVCDFSCQS--VPRSPVCGSDGVTYGTECDLKKARCESQQELYVAAQGACRG 647 HSP 4 Score: 133.265 bits (334), Expect = 3.710e-30 Identity = 143/478 (29.92%), Postives = 198/478 (41.42%), Query Frame = 0
Query: 29 VPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGR-FQCQC-QECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEEC-IEDTNGNPKCLCQGCSNKPSEE--VCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYC----SEGQCIC-RQECPDSYEPICASNGKTYFNYCHLEKDNCARQ------HDANFEQ-------FRVVESRVCLEKV----------------PVCPVDGCTCPHGVGGHLCDRCKTA------FWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGC----------ISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQ 448
PVCGSD TY N CEL+ A C +Q+ I + C + C++ C F C + +G+ C C C+ VCGSD V YP+EC+L +C + I K G C+ CQ + C + +P+ L + N P++ +CG DG+TY + C + R T+ + G C D C E TC+F S C C C R C SY P+CA +G TY N C ++ C +Q H +Q + VC K PVC DG T G +C+ A C CG FGS C+ +TG C C S +C + S C IS+ CGE C FGA CS +C+C C +P VCG+DG TY S C+LR+ +C++Q I + PC+
Sbjct: 102 APVCGSDASTYSNECELQRAQCNQQRRIRLLRQGPCGSRDPCANVTCSFGSTCVPSADGQTASCLCPTTCFGAPDGT--VCGSDGVDYPSECQLLSHACASQEHIFKKFNGPCDPCQGSMSDLNHICRVNPRTRHPEMLLRP-ENCPAQHTPICGDDGVTYENDCVMSRIGATRGLLLQKVRSGQCQTRDQCPE-TCQFNSVCLSRRGRPHCSCDRVTCDGSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVKNGKAECECQRVCSGIYDPVCGSDGVTY-----GSVCELESMACTLGREIQVARRGPCDPCGQCRFGSL---CEVETGRCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAG----QCVCP-RCEHP--PPGPVCGSDGVTYLSACELREAACQQQVQIEEAHAGPCE 560 HSP 5 Score: 127.102 bits (318), Expect = 3.135e-28 Identity = 92/262 (35.11%), Postives = 126/262 (48.09%), Query Frame = 0
Query: 5 LSEDQVPFCACENL-CDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDA-NLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCIC-RQECPDSYEPICASNGKTYFNYCHLEKDNCARQ 263
LS P C+C+ + CD PVC D TY N C ++A C +Q+ I C+ + C C F VC G+ +C+CQ Y+PVCGSD V Y + CEL +C EI V + G C+ C + G C +T +C+C + ++ VCGSDG TY S C L +CT ++ G C C E C FG+ CS GQC+C R E P P+C S+G TY + C L + C +Q
Sbjct: 301 LSRRGRPHCSCDRVTCD-----GSYRPVCAQDGHTYNNDCWRQQAECRQQRAIPPKHQGPCDQTPSPCHGVQCAFGAVCTVK-NGKAECECQRVCSGI--YDPVCGSDGVTYGSVCELESMACTLGREIQVARRGPCDPCGQCRFG--SLCEVETG---RCVCPSECVESAQPVCGSDGHTYASECELHVHACTHQISLYVASAGHCQTCGEKVCTFGAVCSAGQCVCPRCEHPPP-GPVCGSDGVTYLSACELREAACQQQ 548 HSP 6 Score: 112.849 bits (281), Expect = 6.833e-24 Identity = 111/436 (25.46%), Postives = 180/436 (41.28%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQHDL--SKSSISIASFVGNSLMAVLA----PADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEV-DGDRVTGSSKGSLRT----LNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRM---PCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQ-IARNENSFTCSCPVGFFGRLC-EDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQ 970
C PNPCHG C G C CP G +GT CQ L + S +A F G S + + D ++ +++ F +GL+LYN G G+ DF+SL + + R +EF YDLG G V+RS + G W + +R + G L+V DG RV G S S + LN+ E ++IG + + GF+G ++ + L + Q V++ +V ++PC + PC N GSC + +EC CP F+G C++ K+ G + +A + ++ L E PE+ R S L+T ++ L+ R ++AL + ++L L S+PV+ L + + ++ W R+ R+
Sbjct: 1483 CQPNPCHGAAPCRVLSSGGAKCECPLGRSGTFCQTVLETAGSRPFLADFNGFSYLELKGLHTFERDLGEKMALEMVFLARGPSGLLLYN-GQKTDGKGDFVSLAL-----------------------HNRHLEFCYDLGKGAAVIRSKEPIALGTWVRVFLERNGRKGALQVGDGPRVLGESPKSRKVPHTMLNLKEPLYIGGAPD--FSKLARGAAVSSGFSGVIQLVSLRGHQLLTQEH----VLRAVDVSPFADHPCTQALGNPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVE------KSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAER---ADYMALAIVDGH---LQLSYDLGSQPVV--LRSTVKVNTNRWLRIRAHRE 1874 HSP 7 Score: 93.9745 bits (232), Expect = 4.416e-18 Identity = 69/251 (27.49%), Postives = 104/251 (41.43%), Query Frame = 0
Query: 555 NPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSIS-------------------IASFVGNSLMA-------VLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIAL 778
NPC GG+C + + C CP GF+G C+ L + S+ I S + N + A L A+ +L RT A GL+L+ E D+++L I+D ++ YDLGS PVVLRS +V +W + A R H++G L+V + VTGSS L+ +W+G + L + G GF GCL+D+ + + L
Sbjct: 1719 NPCLNGGSCVPREATYECLCPGGFSGLHCEKGLVEKSVGDLETLAFDGRTYIEYLNAVIESELTNEIPAPETLDSRALFSEKALQSNHFELSLRTEATQGLVLWIGKAAE--RADYMALAIVD-----------------------GHLQLSYDLGSQPVVLRSTVKVNTNRWLRIRAHREHREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLQKLPVGQALPKA-YGTGFVGCLRDVVVGHRQLHL 1943
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Match: gi|460018313|sp|P31696.3|AGRIN_CHICK (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22; Flags: Precursor) HSP 1 Score: 184.882 bits (468), Expect = 4.883e-46 Identity = 158/523 (30.21%), Postives = 215/523 (41.11%), Query Frame = 0
Query: 5 LSEDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC-------------------DPCNEVTCKFGSYCSEGQCICRQEC---PDSYEPICASNGKTYFNYCHLEKDNC-ARQHDANFEQFRVVESRVCLEKVPVC------------------------------------------PVDG-CTCPHGVGGHLCDRCKTAFWAFTSF------GCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPL---YYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRIT 452
+ +++ P C C+ +C + R + PVCGSD+ TY N CEL AC ++ I C+ C F +C GR C EC +PVCG+D Y +ECEL +C Q+ I V G C++C C C+ +C+C C +P +VCG+DG+TY + C L+ ASC Q + I G C D C + + +C+C C P S P+C S+ TY N C L+K C RQ+ Q +PV P G C+C GVGG CDRC+ FW F GC C C+ GS DC+ TG+C CK G +G KC CP+G+ +GC S P C E C FGATC G C +C +PL VCG+DG TY +CQL+ +CR+ I + + C IT
Sbjct: 482 VVKNREPVCECQQVC----QGRYD-PVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDRCGKCQ-----FGAICEAE-TGRCVCP-TECVPSS---QPVCGTDGNTYGSECELHVRACTQQKNILVAAQGDCKSCGTTVCSFGSTCV-----GGQCVCPRCEQQPLAQVCGTDGLTYDNRCELRAASCQQQKSIEVAKMGPCEDECGSGGSGSGDGSECEQDRCRHYGGWWDEDAEDDRCVCDFTCLAVPRS--PVCGSDDVTYANECELKKTRCEKRQNLYVTSQGACRALTTTPPPLPVVHCSQTIYGCCPDNMTLALGVGAAGCPSTCQCNPYGSYGGTCDPATGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDSKSGCTPCNCDPVGSVRDDCEQMTGLCSCKTGITGMKCNQCPNGSKMGMAGCEKDPSA----PKSCEEMSCEFGATCVEVNGFAHC----ECPSPLCSEANMTKVCGSDGVTYGDQCQLKTIACRQGQLITVKHVGQCHESIT 974 HSP 2 Score: 167.548 bits (423), Expect = 1.162e-40 Identity = 109/342 (31.87%), Postives = 162/342 (47.37%), Query Frame = 0
Query: 532 SRTCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKN-QGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCE 872
+R S + K +RP CD +PC GGTCE+ F+C CP G G +C+ + I SF G S +A A + + I ++FR +GL+LYN N DF+SL ++ FVE R++ GSG V+ S +V PG+WH+LV R + G+L VDG+ V+G S LN+ +++G V +R +G G ++ L+++ + L+ E+ V+ V +C +PC PC + SC VK + F CEC +TG C +RN C P PC C + E C+CP+G G CE
Sbjct: 1337 TRQPPSTTKKPSRP------CDSHPCLHGGTCEDDGREFTCRCPAGKGGAVCEKPIR---YFIPSFGGKSYLA-FKMMKAYHTVRIAMEFRATELSGLLLYNGQNRGK---DFISLALVGG-----------------------FVELRFNTGSGTGVITSKVRVEPGKWHQLVVNRNRRSGMLAVDGEHVSGESPTGTDGLNLDTDLFVGGAPEDQMAVVAERTAATVGLKGSIRLLDVNNQMYDLR-EKGSDVLYGSGVGECGNDPCHPNPCHHGASCHVKEAEMFHCECLHSYTGPTCADERNPCDPTPCHISATC-LVLPEGGAMCACPMGREGEFCE 1640 HSP 3 Score: 148.288 bits (373), Expect = 9.934e-35 Identity = 130/433 (30.02%), Postives = 192/433 (44.34%), Query Frame = 0
Query: 30 PVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCET-----CQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSE--GQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLE-KVPVCPVDGCTCPHGVGGH-LCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESEC-NFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRIT 452
PVCG D VTY + C + + I ++ QC+ + C D C FN VC + + R C C Y PVC D+ Y N+CE +K+ C QK I VK G C+ C +C C+ N P C CQ + VCGSD TY + C L +C +I +H G CD C + C+FG+ C G+C+C EC S +P+C ++G TY + C L C +Q + V C VC G TC VGG +C RC+ A + CG + +++ C+ + C ++ +K C D S SG S + +R C ++G RC+C+ C R VCG+D TY +EC+L++ C K+ ++ +T+ C+ T
Sbjct: 357 PVCGDDGVTYASECVMGRTGAIRGLEIQKVRSGQCQHQDKCK-DECKFNAVCLKRWHARCSCDRITC---DGTYRPVCARDSRTYSNDCERQKAECHQKAAIPVKHSGPCDLGTPSPCLSVECTFGATCVVK-NREPVCECQQVCQGRYDPVCGSDNRTYGNPCELNAMACVLKREIRVKHKGPCDRCGK--CQFGAICEAETGRCVCPTECVPSSQPVCGTDGNTYGSECELHVRACTQQKN-----ILVAAQGDCKSCGTTVCSF-GSTC---VGGQCVCPRCEQQPLA------QVCGTDGLTYDNR-CELRAASCQQQKSIEVAKMGPCEDECGSGGSGSGDGSECEQDR--------CRHYGGWWDEDAEDDRCVCDFTCL--AVPRSPVCGSDDVTYANECELKKTRCEKRQNLYVTSQGACRALTT 756 HSP 4 Score: 137.117 bits (344), Expect = 2.663e-31 Identity = 143/472 (30.30%), Postives = 205/472 (43.43%), Query Frame = 0
Query: 29 VPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQ-CQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPS--EEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYCSE---GQCIC-RQECPDSYEPICASNGKTYFNYCHLEKDNCARQH---------------------DANFEQFRVVESR--------VCLEKV-PVCPVDGCTC--PHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGC-ISVSSTKYERPI---------PCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQ 448
PVCGSD+ TY N CEL++A C +Q+ I IS C + C+ C F C R+ +G+ C C E VCGSD Y +EC+L K +C ++ + K G C+ C+ + C + L N PS E VCG DG+TY S C + R + +I G C D C + CKF + C + +C C R C +Y P+CA + +TY N C +K C ++ + F VV++R VC + PVC D T P + C K C CG +FG+ C+A+TG C C S +C + S C + V + ++ I CG + C+FG+TC GG +C+C + PL VCGTDG TY + C+LR SC++Q I + PC+
Sbjct: 209 APVCGSDYSTYSNECELEKAQCNQQRRIKVISKGPCGSKDPCAEVTCSFGSTCVRSADGQTAGCVCPASCSGVAE-SIVCGSDGKDYRSECDLNKHACDKQENVFKKFDGACDPCKGILNDMNRVCRVNPRTRRVELLSRPENCPSKREPVCGDDGVTYASECVMGRTGAIRGLEIQKVRSGQCQHQDKCKD-ECKFNAVCLKRWHARCSCDRITCDGTYRPVCARDSRTYSNDCERQKAECHQKAAIPVKHSGPCDLGTPSPCLSVECTFGATCVVKNREPVCECQQVCQGRYDPVCGSDNRTYGNPCELNAMAC-VLKREIRVKHKGPCDRCGKCQFGA---ICEAETGRCVCPTECVPSSQPVCGTDGNTYGSECELHVRACTQQKNILVAAQGDCKSCGTTVCSFGSTC--VGG--QCVCPRCEQQPL---AQVCGTDGLTYDNRCELRAASCQQQKSIEVAKMGPCE 667 HSP 5 Score: 93.2041 bits (230), Expect = 7.204e-18 Identity = 69/252 (27.38%), Postives = 110/252 (43.65%), Query Frame = 0
Query: 554 PNPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISIA---SFVGNSLM-------------------AVLAPAD----AVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIAL 778
PNPC GGTC + C C GF+G C+ + + + A +F G + M A+ PA+ A+ +L +T A GLIL++ LE +D+++L I+D FV+ YDLGS PVVLRS + W + A R ++G L+V + +TGSS L+ +W+G ++ + L + + GF GC++D+ + R + L
Sbjct: 1840 PNPCQNGGTCSPRLESYECACQRGFSGAHCEKVIIEKAAGDAEAIAFDGRTYMEYHNAVTKSHLSNEIPAPDALDYPAEPSEKALQSNHFELSIKTEATQGLILWSGKGLE--RSDYIALAIVDG-----------------------FVQMMYDLGSKPVVLRSTVPINTNHWTHIKAYRVQREGSLQVGNEAPITGSSPLGATQLDTDGALWLGGMERLSVAHKLPKAYS-TGFIGCIRDVIVDRQELHL 2065 HSP 6 Score: 92.4337 bits (228), Expect = 1.431e-17 Identity = 76/295 (25.76%), Postives = 126/295 (42.71%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQHDLSKSSIS--IASFVGNSLMAVLAPADAV---NRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVD-GDRVTGSSKGSLRT----LNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMP--CSNEGSCSVKNQGFECECPPEFTGRRCQR 833
CDP PCH TC +G C CP G G C+ + + F G S + + ++ +++ F + +G+I YN G G+ DF+SL + HD Y +E+RYDLG G VLRS V W ++ +R + G++ ++ G+RV G S S + LN+ E ++G + + F G ++ + + + L EQ + + +PC + P C N G+CS + + +EC C F+G C++
Sbjct: 1607 CDPTPCHISATCLVLPEGGAMCACPMGREGEFCERVTEQDHTMPFLPEFNGFSYLELNGLQTLFLTCRQMSMEVVFLAKSPSGMIFYN-GQKTDGKGDFVSLAL----------------HDGY-------LEYRYDLGKGAAVLRSKEPVPLNTWISVLLERSGRKGVMRINNGERVMGESPKSRKVPHAFLNLKEPFYVGGAPDF--SKLARAAAISTSFYGAVQRISIKG--VPLLKEQH--IRSAVEISTFRAHPCTQKPNPCQNGGTCSPRLESYECACQRGFSGAHCEK 1871
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Match: gi|460018339|sp|O00468.5|AGRIN_HUMAN (RecName: Full=Agrin; Contains: RecName: Full=Agrin N-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 110 kDa subunit; Contains: RecName: Full=Agrin C-terminal 90 kDa fragment; Short=C90; Contains: RecName: Full=Agrin C-terminal 22 kDa fragment; Short=C22; Flags: Precursor) HSP 1 Score: 166.007 bits (419), Expect = 2.954e-40 Identity = 149/515 (28.93%), Postives = 194/515 (37.67%), Query Frame = 0
Query: 30 PVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDP---------------CNEVTCK-----FGSYCSEGQCICR---QECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGC-RECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDG-----TLSTD--SGCISVS-------------------------------------------STKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRSTFDMELKMTLISLM 470
PVCGSD VTY + CEL+ AC + I C+ C F +C GR C EC +PVCGSD YP+EC L +C ++ + V G CETC D C C +C+C C + P VCGSDG+TY S C L+ A+C Q I G C+ C + C+ + +G C+C Q P S P+C S+G TY C L+K C Q PV P+ P+G C TA GC C CN GS CD TG C C+ G G +C+ C G + TD SGC S P C E C FGA C G C+C + P VCG+DG TY +ECQL+ +CR+ I+I + PCQ + + +T L+
Sbjct: 500 PVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCR-----FGALC-EAETGRCVCP-SECVAL---AQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAG-----QCVCPRCEHPPPGPVCGSDGVTYGSACELREAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQSVPGS--PVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRGPTFAPLPPVAPLHCAQTPYGC----CQDNITAARGVGLAGCPSACQCNPHGSYGGTCDPATGQCSCRPGVGGLRCDRCEPGFWNFRGIVTDGRSGCTPCSCDPQGAVRDDCEQMTGLCSCKPGVAGPKCGQCPDGRALGPAGCEADASAPATCAEMRCEFGARCVEESGSAHCVCPM-LTCPEANATKVCGSDGVTYGNECQLKTIACRQGLQISIQSLGPCQEAVAPSTHPTSASVTVTTPGLL 992 HSP 2 Score: 164.081 bits (414), Expect = 1.261e-39 Identity = 130/452 (28.76%), Postives = 193/452 (42.70%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEEH--DGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQR-KNVIDCEENPCVRMPCSNEGSCSVKNQG-FECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLAL----KVSQSGQLV--------MELRETL-ESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHL-DGEKSM 984
CD PC GGTC++ G F+C CP G G +C+ L + +F G S +A A + L + L+FR GL+LYN GN DFL+L ++D V+ R+D GSGP VL S V PGQWH L R+ + G L VDG+ V G S LN+ +++G V L+R G G GC++ L+++ + L P R V +C ++PC+ PC C G F C+CPP G C +++ C PNPC C++ E C CP+G G C+ + + + + + ++ GL T + ++ V L L K G V +E R L + VI S E P++ G W RV++ R + + DG + +
Sbjct: 1333 CDSQPCFHGGTCQDWALGGGFTCSCPAGRGGAVCEKVLGA---PVPAFEGRSFLA-FPTLRAYHTLRLALEFRALEPQGLLLYN-GNARG--KDFLALALLD-----------------------GRVQLRFDTGSGPAVLTSAVPVEPGQWHRLELSRHWRRGTLSVDGETPVLGESPSGTDGLNLDTDLFVGGVPEDQAAVALERTFVGAGLRGCIRLLDVNNQRLELGI--GPGAATRGSGVGECGDHPCLPNPCHGGAPCQNLEAGRFHCQCPPGRVGPTCADEKSPCQPNPCHGAAPCRVL-PEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELR-GLHTFARDLGEKMALEVVFLARGPSGLLLYNGQKTDGKGDFVSLALRDRRLEFRYDLGKGAAVIRSRE---PVTLGAWTRVSLERNGRKGALRVGDGPRVL 1747 HSP 3 Score: 142.124 bits (357), Expect = 7.711e-33 Identity = 126/454 (27.75%), Postives = 186/454 (40.97%), Query Frame = 0
Query: 28 NVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCE----TCQDKKCGPHEECIEDTNGNPKCLC-QGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSE--GQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQ---HDANFEQFRVVESRVCLEKVPVCPVDGCTCP---HGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGAT------------CSNTGGRL-------RCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQG 449
PVCG D VTY N C + + + + ++ QC+ + C +PC FN VC + GR +C C + Y PVC D Y ++C +++ C+Q+ I K G C+ C +C C NG C C Q CS+ + VCGSDG+TY S C L+ +CT +I G CD C + C+FG+ C G+C+C EC +P+C S+G TY + C L C Q H A+ VC VC C CP H G +C + GS CE+ ++ C+ + + R PC ++EC G + C GG C+C+ C++ VCG+DG TY +EC+L++ C Q + + C+G
Sbjct: 353 QAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCP-EPCRFNAVCL-SRRGRPRCSCDRVTCDG-AYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVK-NGQAACECLQACSSL-YDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQ--CRFGALCEAETGRCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGA-VCSAGQCVCPRCEHPPPGPVCGSDGVTY-------------------------------------GSACEL-------REAACLQQTQIEEARAGPCEQAECGSGGSGSGEDGDCEQELCRQRGGIWDEDSEDGPCVCDFSCQS--VPGSPVCGSDGVTYSTECELKKARCESQRGLYVAAQGACRG 752 HSP 4 Score: 138.658 bits (348), Expect = 8.693e-32 Identity = 131/439 (29.84%), Postives = 189/439 (43.05%), Query Frame = 0
Query: 29 VPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEG-RFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEEC-IEDTNGNPKCLC--QGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYC----SEGQCIC-RQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGE-SECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCG 454
PVCGSD TY N CEL+ A C +Q+ I +S C + CS+ C F CAR+ +G C C E VCGSD YP EC+L + +C ++ + K G C+ CQ P C + P+ L + C + VCG DG+TY + C + R+ + + G C D C E C+F + C +C C R C +Y P+CA +G+TY + C ++ C +Q R + S+ P D P C + AF A + + C + S D VC GS CE+ + C + R PC +C FGA C G RC+C +C + + VCG+DG+TY SEC L ++C Q + + + PC+ TCG
Sbjct: 207 APVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGT-VCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPAR-QAPVCGDDGVTYENDCVMGRSGAARGLLLQKVRSGQCQGRDQCPE-PCRFNAVCLSRRGRPRCSCDRVTCDGAYRPVCAQDGRTYDSDCWRQQAECRQQ--------RAIPSKH------QGPCDQAPSP-------CLGVQCAFGATCAVKNGQAACECLQACSSLYDP---VCGSDGVTYGSACEL-------EATACTLGREIQVARKGPCDRCGQCRFGALCEAETG--RCVCPSEC---VALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCE---TCG 603 HSP 5 Score: 123.25 bits (308), Expect = 5.479e-27 Identity = 92/263 (34.98%), Postives = 127/263 (48.29%), Query Frame = 0
Query: 5 LSEDQVPFCACENL-CDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDA-NLCSSDPCPFNGVCARNYEGRFQCQC-QECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCIC-RQECPDSYEPICASNGKTYFNYCHLEKDNCARQ 263
LS P C+C+ + CD PVC D TY + C ++A C +Q+ I C+ A + C C F CA G+ C+C Q C Y+PVCGSD V Y + CEL ++C EI V + G C+ C + G C +T +C+C ++ VCGSDG TY S C L +CT + G C+ C + C FG+ CS GQC+C R E P P+C S+G TY + C L + C +Q
Sbjct: 406 LSRRGRPRCSCDRVTCDGAYR-----PVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVK-NGQAACECLQACSSL---YDPVCGSDGVTYGSACELEATACTLGREIQVARKGPCDRCGQCRFG--ALCEAETG---RCVCPSECVALAQPVCGSDGHTYPSECMLHVHACTHQISLHVASAGPCETCGDAVCAFGAVCSAGQCVCPRCEHPPP-GPVCGSDGVTYGSACELREAACLQQ 653 HSP 6 Score: 119.013 bits (297), Expect = 1.101e-25 Identity = 122/440 (27.73%), Postives = 173/440 (39.32%), Query Frame = 0
Query: 36 HVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYE--GRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKC---ETCQDKKCGPHEECIEDTNG-NPKCLCQG-CSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEV------TCKFGSYCSEGQCICRQE-CPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSST-KYERPIP-------------CGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPC 447
+T RN E++ C + + T + + C C F VC N E GR C C++ PVCGSD Y NECEL+++ C Q+ I + G C + C + C C +G CLC C P VCGSDG Y C L R +C + E++F + G CDPC +C+ + + R E CP P+C +G TY N C + + AR + V S C + D C P C+ + G C C+ CD G+ +C + DS C + + +R IP C +C FGATC+ G+ C C C + LY + VCG+DG TY S C+L +C +I + PC
Sbjct: 140 RITLRNLEEVE--FCVEDKPGTHFTPVPPTPPDACRGMLCGFGAVCEPNAEGPGRASCVCKK-SPCPSVVAPVCGSDASTYSNECELQRAQCSQQRRIRLLSRGPCGSRDPCSNVTCSFGSTCARSADGLTASCLCPATCRGAPEGTVCGSDGADYPGECQLLRRACARQENVFKKFDGPCDPCQGALPDPSRSCRVNPRTRRPEMLLRPESCPARQAPVCGDDGVTYENDCVMGRSGAARGL-----LLQKVRSGQCQGR------DQCPEP----------CRFNAVCLSRRGRPRCSCDRV-----TCD-------------GAYRPVCAQDGRTYDSDCWRQQAECRQQRAIPSKHQGPCDQAPSPCLGVQCAFGATCAVKNGQAACECLQACSS-LY--DPVCGSDGVTYGSACELEATACTLGREIQVARKGPC 534 HSP 7 Score: 112.079 bits (279), Expect = 1.167e-23 Identity = 117/472 (24.79%), Postives = 186/472 (39.41%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQHDLSK--SSISIASFVGNSLMAVLA----PADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEV-DGDRVTGSSKGSLR----TLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLEL-SRTLIALQSEQEPMVMQRKNVIDCEENPCVRM---PCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQ-IARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGEKSMF-PEKLEYLDFVSHGQLTFGPL 1005
C PNPCHG C +G C CP G GT CQ + S +A F G S + + D ++ +++ F +GL+LYN G G+ DF+SL + DR +EFRYDLG G V+RS V G W + +R + G L V DG RV G S S + LN+ E +++G + + GF G ++ + L R L+ + V+++ +V +PC R PC N SC + + C CP F+G C++ K+ G +A + +F L + + + L+T ++ L+S R ++AL + ++L L S+PV+ L + +P++ W RV R+ + + E + L + G L G L
Sbjct: 1592 CQPNPCHGAAPCRVLPEGGAQCECPLGREGTFCQTASGQDGSGPFLADFNGFSHLELRGLHTFARDLGEKMALEVVFLARGPSGLLLYN-GQKTDGKGDFVSLALRDR-----------------------RLEFRYDLGKGAAVIRSREPVTLGAWTRVSLERNGRKGALRVGDGPRVLGESPKSRKVPHTVLNLKEPLYVGGAPD--FSKLARAAAVSSGFDGAIQLVSLGGRQLLTPE-----HVLRQVDVTSFAGHPCTRASGHPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVE------KSAGDVDTLAFDGRTFVEYLNAVTESELANEIPVPETLDSGALHEKALQSNHFELSLRTEATQGLVLWSGKATER---ADYVALAIVDGH---LQLSYNLGSQPVV--LRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGL 2018 HSP 8 Score: 88.1965 bits (217), Expect = 2.544e-16 Identity = 71/275 (25.82%), Postives = 110/275 (40.00%), Query Frame = 0
Query: 555 NPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDL---SKSSISIASFVGNSLMAVLAPADAVNRLEI-------------------------KLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPC 800
+PC G +C + + C CP GF+G C+ L S + +F G + + L +AV E+ +L RT A GL+L++ E D+++L I+D ++ Y+LGS PVVLRS V +W +VA R ++G L+V + VTGSS L+ +W+G + L + G GF GCL+D+ + R P+ + V E PC
Sbjct: 1828 HPCLNGASCVPREAAYVCLCPGGFSGPHCEKGLVEKSAGDVDTLAFDGRTFVEYL---NAVTESELANEIPVPETLDSGALHEKALQSNHFELSLRTEATQGLVLWSGKATE--RADYVALAIVDG-----------------------HLQLSYNLGSQPVVLRSTVPVNTNRWLRVVAHREQREGSLQVGNEAPVTGSSPLGATQLDTDGALWLGGLPELPVGPALPKA-YGTGFVGCLRDVVVGR---------HPLHLLEDAVTKPELRPC 2064
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Match: gi|2494281|sp|Q90404.1|AGRIN_DIPOM (RecName: Full=Agrin) HSP 1 Score: 141.739 bits (356), Expect = 7.827e-33 Identity = 99/335 (29.55%), Postives = 146/335 (43.58%), Query Frame = 0
Query: 542 DTRP--IHVSGLCDPNPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGD-RVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKN-QGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCE 872
+ RP + V CD PC GGTCE+ ++C CP G G +C+ + I F G S +A A + I ++FR +GL P++ T + ++ P +VE R++ G V+ S + PG WH +V R + G+L VDG+ + G S LN+ +++G V +R G GC++ L+++ + LQ E+ V+ V +C NPC PC N G C +K + F CE EF+G C K N C PNPC C + E C CP+G G LCE
Sbjct: 600 EVRPATVKVHRPCDSQPCLHGGTCEDDGVSYTCSCPAGRGGAVCERTIV---YFIPEFGGRSYLA-FKTMKAYYTVRISMEFRASNLDGL--------------------------PLVQWTEKGKGLHFYRPSEGYVELRFNHGVWDGVITSKTLIKPGNWHHVVGNRNRRSGMLSVDGEPHLIGESPPGTDGLNLDTDLFLGGTPEDEMTLVTERTTATKGLQGCIRLLDVNNLIYDLQ-ERSNDVLYGSGVGECGNNPCSPNPCKNRGKCHMKEAEMFHCESVGEFSGPTCADKHNPCDPNPCHQSANCMVLP-EGGSKCECPMGREGELCE 902 HSP 2 Score: 118.627 bits (296), Expect = 9.382e-26 Identity = 100/302 (33.11%), Postives = 129/302 (42.72%), Query Frame = 0
Query: 172 PSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCN--------EVTCKFGSY------CSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDG-CTCPHGVGGHLCDRCKTAFWAFTSF------GCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGC---ISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQG 449
P VCGSDG+TY S C L+ C +D+ H PC E+ C Y + Q + CP + C N Y +Y S+ C P G C+C GVGG CDRC+ FW F GC C C G+ DC+ +G+C CK G SG KC CP+G+ SGC SVS T C + C +GATC + GR C C P + VCG+DG TY +ECQL+ +CR+ + I I + PCQG
Sbjct: 2 PLSPVCGSDGVTYDSECALKLMRCMIQKDL---HVVMLSPCKDASPSSVPELHCSRSVYGCCRDNVTAAQGVGLAGCPST----CECN--RYGSY-----------------------SKTC------SPSSGQCSCKPGVGGLKCDRCEPGFWNFRGIVTDEKSGCTPCNCYPLGAVRDDCEQMSGLCSCKAGISGMKCNQCPNGSKLGPSGCDQDPSVSRT-------CSDLHCQYGATCVQSIGRAYCECPPSI-CPKNKQFKVCGSDGVTYANECQLKTIACRQGSVINILHQGPCQG 257 HSP 3 Score: 109.383 bits (272), Expect = 8.011e-23 Identity = 109/438 (24.89%), Postives = 185/438 (42.24%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQH----DLSKSSISIASFVGNSLMAV----LAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLR----TLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPC--VRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDR----QKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILR 969
CDPNPCH C +G C CP G G LC+ + + I F G S + + +D + +L +++ F NG+I YN G G DF+SL + D ++EF+YDLG G VLRS A + W+ + +R + GL++++ D V+G S S + LN+ E ++G + + G GFTG ++ L L +++ L+ E M+ N + C R PC N G CS + + ++C C F+G +C++ A E S + S V F GR ++ Y + ++ ++ ++T +K L+S + + ++AL V +++ L S+PV +L + +P++ W R+ R
Sbjct: 869 CDPNPCHQSANCMVLPEGGSKCECPMGREGELCERVSEAEQDQGKAFIPEFNGLSYLEMNGIHTFVSDLLQKLSMEVIFLAKDPNGMIFYN-GQKTDGRGDFVSLNLRDG-----------------------YLEFKYDLGKGAAVLRSKAPIPLNVWNVVTVERNGRKGLMKINKDELVSGESPKSRKAPHTALNLKEAFYVGGAPDF--NKFARAAGIISGFTGAIQKLSL-KSIPLLKKENIRNAMEISNF---RWHACTKTRNPCQNGGVCSPRLREYDCMCQRGFSGPQCEK------------------ALEEKSASGSESVAFNGR---------TFIEYHNTVTRSEKAVQVNYFEMSIKTEATKGLILWSGKIAEK---SDYIALAVVDG---FVQMTYDLGSKPV--TLRSTIPVNTNQWVRIKANR 1241 HSP 4 Score: 97.4413 bits (241), Expect = 3.776e-19 Identity = 68/232 (29.31%), Postives = 104/232 (44.83%), Query Frame = 0
Query: 555 NPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLE-------IKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIAL 778
NPC GG C + C C GF+G C+ L + S S + V + + + V R E ++ +T A GLIL++ E ++D+++L ++D FV+ YDLGS PV LRS V QW + A R H G L+V + VTGSS + L+ +W+G ++ N L + + GF GC+KD+ + R + L
Sbjct: 1106 NPCQNGGVCSPRLREYDCMCQRGFSGPQCEKALEEKSASGSESVAFNGRTFIEYHNTVTRSEKAVQVNYFEMSIKTEATKGLILWSGKIAE--KSDYIALAVVDG-----------------------FVQMTYDLGSKPVTLRSTIPVNTNQWVRIKANRIHGYGTLQVGNEAPVTGSSPFAATQLDTDGALWLGGIEKLAPGNRLPKAYS-TGFIGCIKDVVIDRQELQL 1311
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Match: gi|1172451|sp|Q05793.1|PGBM_MOUSE (RecName: Full=Basement membrane-specific heparan sulfate proteoglycan core protein; Short=HSPG; Contains: RecName: Full=Endorepellin; Contains: RecName: Full=LG3 peptide; Flags: Precursor) HSP 1 Score: 130.183 bits (326), Expect = 4.220e-29 Identity = 116/442 (26.24%), Postives = 192/442 (43.44%), Query Frame = 0
Query: 570 FSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDC-EENPCVRMPCSNEGSCSVKNQ-GFECECPPEFTGRRCQRKRNHCHPN-PCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDP----ESSWERYRKSTSKYDRQKVSFGLQTN-LSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVME--LRETLESEPVI-------------------ASLETNLPISDGNWHRVNILRQDSSLIVHLDGE 981
++C C G +G C+ ++ ++ S++ S +A+ A + + L + ++F+ NG++L++ G E DF+SL ++ +EFRY+LGSG VLRS+ + G+WH + A+R +KDG L VDG R V SS G + LN+ L+++G V+ + + F GC+ ++ ++ + L + + V C + +PC R PC N +C + F+C C F G C+ + N C + PC N G C+ AR C C GF G C+ ES W + Q ++ L + FS + P + + G L+ + +RE S+ I A L + PI+DG WHR+ LR+ + +DGE
Sbjct: 3225 YTCRCHLGRSGVRCEEGVTVTTPSMSG--AGSYLALPALTNTHHELRLDVEFKPLEPNGILLFSGGKSGPVE-DFVSLAMVGG-----------------------HLEFRYELGSGLAVLRSHEPLALGRWHRVSAERLNKDGSLRVDGGRPVLRSSPGKSQGLNLHTLLYLGGVEPSVQ--LSPATNMSAHFHGCVGEVSVNGKRLDLTYS----FLGSQGVGQCYDSSPCERQPCRNGATCMPAGEYEFQCLCQDGFKGDLCEHEENPCQLHEPCLNGGTCRGAR------CLCLPGFSGPRCQQGAGYGVVESDWHPEGSGGNDAPGQYGAYFYDNGFLGLPGNSFSRSLPEVPETIEFEVRTSTADGLLLWQGVVREASRSKDFISLGLQDGHLVFSYQLGSGEARLVSGDPINDGEWHRITALREGQRGSIQVDGE 3628 HSP 2 Score: 90.5077 bits (223), Expect = 6.524e-17 Identity = 101/423 (23.88%), Postives = 163/423 (38.53%), Query Frame = 0
Query: 601 SLMAVLAPADAVNRLEIKLKFRTFAQNGLILYN-----EGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDG-DRVTGSSKGSLRTLNVGELIWIGNVDTQYED-NVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCS-VKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNEN-SFTCSCPVGFFGRLCEDS--------DPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGEKSMF---PEKLEYLDFVSHGQLTFG 1003
S + + DA + EIK+ FR + +G++LYN NL + DF+S ++ EFR+D GSG +R + GQ+H + R G L V V G+S+G + L++ E +++G Y D + + G GF GC+++L + I + + + C C PC N G C ++ + C CP FT R C P + R + +TC C +G G CE+ S+ T+ + ++ + LFS + G V G L E R L S +A L ++ P++ G WHRV+ R + + +DG + + P K + L+ H L G
Sbjct: 2993 SFLPLPTIKDAYRKFEIKITFRPDSADGMLLYNGQKRSPTNLANRQPDFISFGLVGGRP-----------------------EFRFDAGSGMATIRHPTPLALGQFHTVTLLRSLTQGSLIVGNLAPVNGTSQGKFQGLDLNEELYLGG----YPDYGAIPKAGLSSGFVGCVRELRIQGEEIVFHD----VNLTTHGISHCPT--CQDRPCQNGGQCQDSESSSYTCVCPAGFTAAAVN-IRKPCTATPSLWADATCVNRPDGRGYTCRCHLGRSGVRCEEGVTVTTPSMSGAGSYLALPALTNTHHELRLDVEFKPLEPNGILLFS---GGKSGPVEDFVSLAMVGGHL--EFRYELGSG--LAVLRSHEPLALGRWHRVSAERLNKDGSLRVDGGRPVLRSSPGKSQGLNL--HTLLYLG 3372 HSP 3 Score: 76.2554 bits (186), Expect = 1.325e-12 Identity = 72/270 (26.67%), Postives = 104/270 (38.52%), Query Frame = 0
Query: 556 PCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISI-----------------ASFVGNSLMAVLAPADAVNRL------EIKLKFRTFAQNGLILYNEGNLETGET-DFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDG-DRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPC 800
PC GGTC C C PGF+G CQ + A F N + + P ++ +R I+ + RT +GL+L+ E + DF+SL + D + F Y LGSG L S + G+WH + A R + G ++VDG D VTG S G +N ++I+IG + L R G TGC+K+L L Q P+ +Q + PC
Sbjct: 3469 PCLNGGTCRG----ARCLCLPGFSGPRCQQGAGYGVVESDWHPEGSGGNDAPGQYGAYFYDNGFLGL--PGNSFSRSLPEVPETIEFEVRTSTADGLLLWQGVVREASRSKDFISLGLQDG-----------------------HLVFSYQLGSGEARLVSGDPINDGEWHRITALREGQRGSIQVDGEDLVTGRSPGPNVAVNTKDIIYIGGAP---DVATLTRGKFSSGITGCIKNLVLHTARPGAPPPQ-PLDLQHRAQAGANTRPC 3705
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Match: gi|81908066|sp|Q4VBE4.1|EGFLA_MOUSE (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; AltName: Full=Nectican; Flags: Precursor) HSP 1 Score: 129.028 bits (323), Expect = 5.113e-29 Identity = 126/441 (28.57%), Postives = 194/441 (43.99%), Query Frame = 0
Query: 549 SGLCDPNPCHGGGTCEEHDG-VFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAV------LAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRVT-GSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRK-RNHCHPNPCKNKGQCQIARNENSFTCSCPV-GFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLD 979
SG+CD C GGTC + C CP GF G C+ + ++I F SL + L P ++ E ++ FR + +G++LY+ +TG DFLS+ + H VEFR+D GSG VLRS A + GQWH+L R K+G+L+VD +V G ++G + I+IG V D+V K G F+G ++ + L+ I ++ + V ++ +PCV PC++ GSC + +G+EC+CP F G CQ++ NHC N+ + + F GR D + +R S R +T L+ +RP N ++L + + G L+ L S +AS+ N SDG WHRV +R S + +D
Sbjct: 566 SGICDEASCIHGGTCAAIKADSYICLCPLGFRGRHCEDAFA---LTIPQFR-ESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYS---YDTGSKDFLSINM-------------AAGH----------VEFRFDCGSGTGVLRSEAPLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNY--DDVKKNSGILHPFSGSIQKIILNDRTIHVKHDFTSGVN-----VENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNHC----------------LNTIIEAIEIPQFIGRSYLTYDNPNILKRVSGS-----RSNAFMRFKTTAKDGLLLWRGDSPMRP-NSDFISLGL-RDGALIFSY--NLGSG--VASIMVNGSFSDGRWHRVKAVRDGQSGKITVD 942 HSP 2 Score: 97.0561 bits (240), Expect = 3.665e-19 Identity = 68/263 (25.86%), Postives = 120/263 (45.63%), Query Frame = 0
Query: 572 CYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEV-DGDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSC-SVKNQGFECECPPEFTGRRCQ 832
C+C G G C D+ I F G+S + ++ ++ L+FR A++GL+LY G E G DF+SL +I R + FR++ G+G ++ S ++ G WH + R +G+L++ +G VTG S+G + +++G + Y +++ GT GF GC++ L ++ I ++ + +V +C C C + G+C ++K + C CP F GR C+
Sbjct: 369 CHCNLGKGGEACSEDIF---IQYPQFFGHSYVTFEPLKNSYQAFQVTLEFRAEAEDGLLLYC-GESEHGRGDFMSLALIRRS-----------------------LHFRFNCGTGIAIIISETKIKLGAWHTVTLYRDGLNGMLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYW--LVRATGTNRGFQGCVQSLSVNGKKIDMRPWPLGKALNGADVGECSSGICDEASCIHGGTCAAIKADSYICLCPLGFRGRHCE 602 HSP 3 Score: 73.1738 bits (178), Expect = 8.229e-12 Identity = 60/223 (26.91%), Postives = 95/223 (42.60%), Query Frame = 0
Query: 538 LSHKDTRPIHVSGLCDP---NPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSK-------SSISIASFVGNSLMAVLAP----ADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVD--GDRVTGSSKGSLRTLNVGELIWIGNV 744
+ H T ++V P PC GG+C + C CP GF G CQ + +I I F+G S + P + +R ++F+T A++GL+L+ + +DF+SL + D + F Y+LGSG + N G+WH + A R + G + VD G R TG S G +R LN+ +++G +
Sbjct: 771 VKHDFTSGVNVENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNHCLNTIIEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGA-----------------------LIFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGAR-TGKSPGLMRQLNINGALYVGGM 969
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Match: gi|158705944|sp|Q63HQ2.2|EGFLA_HUMAN (RecName: Full=Pikachurin; AltName: Full=Agrin-like protein; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor) HSP 1 Score: 129.028 bits (323), Expect = 5.619e-29 Identity = 136/496 (27.42%), Postives = 212/496 (42.74%), Query Frame = 0
Query: 549 SGLCDPNPCHGGGTCEEHDG-VFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAV------LAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRVT-GSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRK-RNHCHPNPCKNKGQCQIARNENSFTCSCPV-GFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLD---GEKSMFPEKLEYLDFVSHGQLTFGPLT----------MRSL--CLRHYVLKT 1019
SG+CD C GGTC + C CP GF G C+ +++I F SL + L P ++ +E ++ FR + +G++LY + +TG DFLS+ + H VEFR+D GSG VLRS + G WHEL R K+G+L+VD ++ G ++G + I+IG V D+V K G F+G ++ + L+ I ++ + V ++ +PCVR PC++ GSC + +G++C+CP F G CQ++ N+C N+ + + F GR D +R S R V +T L+ +RP N ++L + + G LV L S +AS+ N +DG WHRV +R S + +D P + L+ +G L G + MR L C+ H+ L T
Sbjct: 566 SGICDEASCIHGGTCTAIKADSYICLCPLGFKGRHCEDAF---TLTIPQFR-ESLRSYAATPWPLEPQHYLSFMEFEITFRPDSGDGVLLY---SYDTGSKDFLSINLAG-------------GH----------VEFRFDCGSGTGVLRSEDPLTLGNWHELRVSRTAKNGILQVDKQKIVEGMAEGGFTQIKCNTDIFIGGVPNY--DDVKKNSGVLKPFSGSIQKIILNDRTIHVKHDFTSGVN-----VENAAHPCVRAPCAHGGSCRPRKEGYDCDCPLGFEGLHCQKECGNYCL----------------NTIIEAIEIPQFIGRSYLTYDNPDILKRVSGS-----RSNVFMRFKTTAKDGLLLWRGDSPMRP-NSDFISLGL-RDGALVFSY--NLGSG--VASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNI--NGALYVGGMKEIALHTNRQYMRGLVGCISHFTLST 995 HSP 2 Score: 99.3673 bits (246), Expect = 7.301e-20 Identity = 98/422 (23.22%), Postives = 166/422 (39.34%), Query Frame = 0
Query: 572 CYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEV-DGDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSC-SVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPV---------IASLETNLPISDGNWHRVNILRQDSSLIVHLDGEK 982
C C G G C D+ I F G+S + ++ +I L+FR A++GL+LY G E G DF+SL II R ++FR++ G+G ++ S ++ G WH ++ R +GLL++ +G VTG S+G + +++G + Y +++ GT GF GC++ L ++ I ++ + +V +C C C + G+C ++K + C CP F GR C E++FT + P ++R+S Y Q LS + RP + + L +G L L V L + P++ GNWH + + R + I+ +D +K
Sbjct: 369 CQCTLGKGGESCSEDIV---IQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYC-GENEHGRGDFMSLAIIRRS-----------------------LQFRFNCGTGVAIIVSETKIKLGGWHTVMLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYW--LVRATGTNRGFQGCVQSLAVNGRRIDMRPWPLGKALSGADVGECSSGICDEASCIHGGTCTAIKADSYICLCPLGFKGRHC-----------------------EDAFTLTIP------------------QFRESLRSYAATPWPLEPQHYLS----FMEFEITFRPDSGDGVLLYSYDTGSKDF-LSINLAGGHVEFRFDCGSGTGVLRSEDPLTLGNWHELRVSRTAKNGILQVDKQK 715 HSP 3 Score: 77.0258 bits (188), Expect = 5.408e-13 Identity = 67/254 (26.38%), Postives = 106/254 (41.73%), Query Frame = 0
Query: 538 LSHKDTRPIHVSGLCDP---NPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSK-------SSISIASFVGNSLMAVLAPADAVNRLE-----IKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVD--GDRVTGSSKGSLRTLNVGELIWIGNVD--TQYEDNVLKRVGTGIGFTGCLKDLELS 772
+ H T ++V P PC GG+C + C CP GF G CQ + +I I F+G S + P D + R+ + ++F+T A++GL+L+ + +DF+SL + D + F Y+LGSG + N G+WH + A R + G + VD G R TG S G +R LN+ +++G + + + R G GC+ LS
Sbjct: 771 VKHDFTSGVNVENAAHPCVRAPCAHGGSCRPRKEGYDCDCPLGFEGLHCQKECGNYCLNTIIEAIEIPQFIGRSYLTYDNP-DILKRVSGSRSNVFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGA-----------------------LVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGAR-TGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMR-----GLVGCISHFTLS 994
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Match: gi|158705693|sp|A3KN33.1|EGFLA_BOVIN (RecName: Full=Pikachurin; AltName: Full=EGF-like, fibronectin type-III and laminin G-like domain-containing protein; Flags: Precursor) HSP 1 Score: 125.176 bits (313), Expect = 9.163e-28 Identity = 132/492 (26.83%), Postives = 202/492 (41.06%), Query Frame = 0
Query: 549 SGLCDPNPCHGGGTCEEHDG-VFSCYCPPGFAGTLCQHDLSKS----SISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLD---GEKSMFPEKLEYLDFVSHGQLTFGPLT----------MRSL--CLRHYVLKT 1019
SG+CD C GGTC + C CP GF G C+ + + S+ S+ L P ++ +E ++ FR +++G++LY + +TG DFLS+ + H VEFR+D GSG VLRS + G WHEL R K+G+L+VD + V G ++G + I+IG V D+V K G F+G ++ + L+ I ++ + V ++ +PCV PC++ GSC + +G+EC+CP F G CQ+ P F GR D +R S R +T L+ +RP N ++L + + G LV L S +AS+ N +DG WHRV +R S + +D P + L+ +G L G + MR L C+ H+ L T
Sbjct: 575 SGICDEASCINGGTCMASKADSYICLCPLGFRGRHCEDAFTLTIPQFKESLRSYAATPW--PLEPRHYLSFMEFEVTFRPDSEDGVLLY---SYDTGSKDFLSINMAG-------------GH----------VEFRFDCGSGTGVLRSEEPLTLGHWHELCVSRTAKNGILQVDKQKAVEGMAEGGFTQIKCNSDIFIGGVPNY--DDVKKNSGILKPFSGSIQKIILNDRTIHVKHDFTWGVN-----VENAAHPCVGSPCAHGGSCRPRKEGYECDCPLGFEGLHCQKAITEAIEIPQ----------------------FIGRSYLTYDNPDILKRVSGS-----RSNAFMRFKTTAKDGLLLWRGDSPMRP-NSDFISLGL-RDGALVFSY--NLGSG--VASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNI--NGALYVGGMKEIALHTNRQYMRGLVGCISHFTLST 996 HSP 2 Score: 97.8265 bits (242), Expect = 2.353e-19 Identity = 71/263 (27.00%), Postives = 120/263 (45.63%), Query Frame = 0
Query: 572 CYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEV-DGDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSC-SVKNQGFECECPPEFTGRRCQ 832
C+C G G C D+ I F G+S + ++ +I L+FR A++GL+LY G E G DF+SL +I R ++FR++ G+G ++ S ++ G WH + R +GLL++ +G VTG S+G + +++G + Y +++ GT GF GC++ L ++ + L+ + +V +C C C N G+C + K + C CP F GR C+
Sbjct: 378 CHCNLGKGGESCSEDIV---IQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYC-GENEHGRGDFMSLAVIRRS-----------------------LQFRFNCGTGVAIIVSETKIKLGGWHTVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYW--LVRATGTNRGFQGCVQALTVNGKRLDLRPWPLGKALSGADVGECSSGICDEASCINGGTCMASKADSYICLCPLGFRGRHCE 611
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Match: gi|317373536|sp|P98160.4|PGBM_HUMAN (RecName: Full=Basement membrane-specific heparan sulfate proteoglycan core protein; Short=HSPG; AltName: Full=Perlecan; Short=PLC; Contains: RecName: Full=Endorepellin; Contains: RecName: Full=LG3 peptide; Flags: Precursor) HSP 1 Score: 124.405 bits (311), Expect = 2.343e-27 Identity = 137/535 (25.61%), Postives = 217/535 (40.56%), Query Frame = 0
Query: 521 ICDENHDYHADSRTCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEEHDGV-FSCYCPPGFAGTLCQHD------------------------------LSKS--------SISIASFVG-NSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIG--FTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDC-EENPCVRMPCSNEGSCSVKNQ-GFECECPPEFTGRRCQRKRNHCH-PNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSD----PESSWERYRKSTSKYDRQ-KVSFGLQTNLSKAADLFS---------IPVRLRPGNVGHLAL----KVSQSGQLVMELRETLESEPVI---------ASLETNLPISDGNWHRVNILRQDSSLIVHLDGEK 982
I E +H + T +SH T C PC GG C + + + C CP GF G+ C+H L +S +++ S G S +A+ A + + L + ++F+ A +G++L++ G E DF+SL ++ +EFRY+LGSG VLRS + G+WH + A+R +KDG L V+G R V SS G + LN+ L+++G V E +V T + F GC+ ++ ++ + L + + + C + +PC R PC + +C + F+C C F G C+ + N C PC + G CQ R C C GF G C+ ES W + Q F L+ +FS I + +R L L +V ++GQ + L+ ++ A L + PI+DG WHRV LR+ + +DGE+
Sbjct: 3825 IQGEEIVFHDLNLTAHGISHCPT--------CRDRPCQNGGQCHDSESSSYVCVCPAGFTGSRCEHSQALHCHPEACGPDATCVNRPDGRGYTCRCHLGRSGLRCEEGVTVTTPSLSGAGSYLALPALTNTHHELRLDVEFKPLAPDGVLLFSGGKSGPVE-DFVSLAMVGG-----------------------HLEFRYELGSGLAVLRSAEPLALGRWHRVSAERLNKDGSLRVNGGRPVLRSSPGKSQGLNLHTLLYLGGV----EPSVPLSPATNMSAHFRGCVGEVSVNGKRLDLTYS----FLGSQGIGQCYDSSPCERQPCQHGATCMPAGEYEFQCLCRDGFKGDLCEHEENPCQLREPCLHGGTCQGTR------CLCLPGFSGPRCQQGSGHGIAESDWHLEGSGGNDAPGQYGAYFHDDGFLAFPGHVFSRSLPEVPETIELEVRTSTASGLLLWQGVEVGEAGQGKDFISLGLQDGHLVFRYQLGSGEARLVSEDPINDGEWHRVTALREGRRGSIQVDGEE 4313 HSP 2 Score: 107.457 bits (267), Expect = 3.874e-22 Identity = 105/426 (24.65%), Postives = 168/426 (39.44%), Query Frame = 0
Query: 601 SLMAVLAPADAVNRLEIKLKFRTFAQNGLILYN--------EGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEV-DGDRVTGSSKGSLRTLNVGELIWIGNVDTQYED-NVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSC-SVKNQGFECECPPEFTGRRCQRKRN-HCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDS--------DPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGEKSMF---PEKLEYLDFVSHGQLTFG 1003
S + + DA + EIK+ FR + +G++LYN NL + DF+S ++ EFR+D GSG +R + G +H + R G L V D V G+S+G + L++ E +++G Y D + + G GF GC+++L + I + + + C C PC N G C ++ + C CP FTG RC+ + HCHP C C + +TC C +G G CE+ S+ T+ + ++ + LFS + G V G L E R L S +A L + P++ G WHRV+ R + + ++G + + P K + L+ H L G
Sbjct: 3672 SFLPLPTIKDAYRKFEIKITFRPDSADGMLLYNGQKRVPGSPTNLANRQPDFISFGLVGGRP-----------------------EFRFDAGSGMATIRHPTPLALGHFHTVTLLRSLTQGSLIVGDLAPVNGTSQGKFQGLDLNEELYLGG----YPDYGAIPKAGLSSGFIGCVRELRIQGEEIVFHD----LNLTAHGISHCPT--CRDRPCQNGGQCHDSESSSYVCVCPAGFTGSRCEHSQALHCHPEACGPDATCVNRPDGRGYTCRCHLGRSGLRCEEGVTVTTPSLSGAGSYLALPALTNTHHELRLDVEFKPLAPDGVLLFS---GGKSGPVEDFVSLAMVGGHL--EFRYELGSG--LAVLRSAEPLALGRWHRVSAERLNKDGSLRVNGGRPVLRSSPGKSQGLNL--HTLLYLG 4055 HSP 3 Score: 72.7886 bits (177), Expect = 1.453e-11 Identity = 74/273 (27.11%), Postives = 108/273 (39.56%), Query Frame = 0
Query: 556 PCHGGGTCEEHDGVFSCYCPPGFAGTLCQ----HDLSKSSISIASFVGNSLMA-----------VLAPADAVNRL------EIKLKFRTFAQNGLILYNEGNLETGET----DFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGI--GFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPC 800
PC GGTC+ C C PGF+G CQ H +++S + GN + P +R I+L+ RT +GL+L+ +E GE DF+SL + D + FRY LGSG L S + G+WH + A R + G ++VDG+ V+G S G +N ++IG +V G G TGC+K+L L Q P+ +Q + PC
Sbjct: 4152 PCLHGGTCQG----TRCLCLPGFSGPRCQQGSGHGIAESDWHLEGSGGNDAPGQYGAYFHDDGFLAFPGHVFSRSLPEVPETIELEVRTSTASGLLLWQ--GVEVGEAGQGKDFISLGLQDG-----------------------HLVFRYQLGSGEARLVSEDPINDGEWHRVTALREGRRGSIQVDGEELVSGRSPGPNVAVNAKGSVYIGGA-----PDVATLTGGRFSSGITGCVKNLVLHSARPGAPPPQ-PLDLQHRAQAGANTRPC 4389
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Match: gi|908458968|ref|XP_013084749.1| (PREDICTED: agrin-like isoform X4 [Biomphalaria glabrata]) HSP 1 Score: 437.958 bits (1125), Expect = 3.783e-130 Identity = 318/1033 (30.78%), Postives = 483/1033 (46.76%), Query Frame = 0
Query: 31 VCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKC-ETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFE-----QFRVVESRVC----LEKVPVC--PVDG-CTCPHGVGGHLCDRCKTAFWAFTSFG------CRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGR-----ITCGRSTFDMELKMTLISLMTDAPDQIYGQS--KSPRQV-LSDQKPSTGQFGET-CLTDIDCIVDNSHCSLNQCICDENHDYHADSRTCKSLSHKDTRPIHVS-GLCDPNPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELS-----RTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKN-QGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCE-DSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAA-------DLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGEKSMFPEKLEYLDFVSHGQLTFGPLTMRSLCLRHYVLKT 1019
VCG+D +TY N CEL+++AC ++ N+T D C +CS+ C + +C + G+ C+C +C EE Y PVCG + + Y N+C+L+ +CQ++ + G C +TC +++C + C E N PKC+C + + VCG+DG+TY + C ++ SC + I G CD C VTC F + C G C+C C EP+CAS+G Y N C ++K +C++ + ++ VC L + PV CTC GV G CDRC+ +W G C C CN+FG+ DCD TG C CK G KC+ C +G + GC +S P+ C E +C F A C G C C+ +C + + VCGTDG T+ S CQL +CR Q DI + N PC+G T T D + + T + D + K+P + D TG + E CL++ DC +SHC C C E + + D+ C + K T IH S C NPC G + + C CP G G LC + + SF G S + V A L I++ F T ++G+IL++ N + G DF+SL + D +VEFRYDLG+GP +LRS + P + H ++A+R + G+L VD ++ V+GSS +L +L++G+ +++G + Q V +VG +G GC+ L RT S+ + ++ +C++NPC MPC NEG+C V + Q FEC+C P F G+ C N C PC + G C + + F C C + G CE +S PE ++ + Y + + T+LS D + V +P G + ++ L+ T + +E+ I WH+V I R + +D + E ++++V G T + R L L Y LK+
Sbjct: 117 VCGTDGITYDNECELRKSACAEKVNVTFKHDGPCGPNQVCSTKVCHYGSMCVE-HAGKAVCRCPQCTEE---YNPVCGDNGITYQNDCKLKLENCQREENVKESHKGACADTCGNQRCHNYAIC-ERVNNKPKCICPTSCVQVDDPVCGNDGVTYANECEMRVESCRTQKFIGIGSKGTCDKCRNVTCNFHAKCENGVCVCPILCATVNEPVCASDGNDYHNECEMKKYSCSQNVFLSVSSPGRCEWPSGSGDVCKCNSLGSISTTCDPVTKQCTCKPGVSGKKCDRCEMGYWGLHKIGEVGNSGCIPCSCNKFGASRDDCDQMTGRCMCKPHVEGFKCDRCLNGKVLGPGGCQDLS------PMSCYELDCRFEAVCREKDGTPFCDCDNNCDYDTHPSDIVCGTDGQTFGSLCQLEYYACRFQQDITVANKGPCRGSSSVLTTTASPDTTDTKSRKTTRHIEGSGGDTYIDDNTIKNPYETEKEDVMTCTGSYIENLCLSNKDCCPTHSHCHRGLCYCKEGYVPNGDNTKCYEIKAKPTETIHSSINACTNNPCQAGTCILDKQLGYRCACPLGKIGPLCNR---AAEFATPSFSGRSHLEVRRIEKATQELSIEVDFSTLNKDGIILFHPQNRD-GTGDFVSLAVND-----------------------GYVEFRYDLGAGPAILRSRDTIEPIRVHRVIARRSKEAGILIVDKEKSVSGSSPPTLTSLDLGDSLYLGYM-PQASREVYDKVGVDLGLVGCIHSLRAGNPGEMRTYKLDYSKGLSDIKAGVDITECDKNPCKSMPCQNEGTCIVLDAQHFECQCSPGFKGKTCDTIVNPCASQPCLHGGTCVPVSSSDGFYCQCAQSYEGSKCERESIPEVFVPQF--TGDSYLEIPLPEIMTTSLSITVWFMTTKPDGLILLVTQKPRGDGDFIALMLENSSLIY----TFYLGSGVGRIESKKHIHLDKWHKVTISRNGRIGQMTIDAHAPITGEA-TFVNYV--GPSTALNIDKRPLYLGGYNLKS 1101 HSP 2 Score: 108.227 bits (269), Expect = 5.295e-20 Identity = 68/193 (35.23%), Postives = 94/193 (48.70%), Query Frame = 0
Query: 102 EYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKC----------------------ETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDP---CNEVTCKFGSYCSE--GQCICR-QECPDSYEPICASNGKTYFNYCHLEKDNCARQHDA 266
E VCG+D Y N C LR+ +C+ K EI V GKC C KCG HEEC +G CLC VCG+DGITY + C L++++C + ++ +H G C P C+ C +GS C E G+ +CR +C + Y P+C NG TY N C L+ +NC R+ +
Sbjct: 21 EINLVCGTDGRTYSNSCFLRQQACKSKKEIRVLNSGKCGPGLTILKGTIQDEYNRKLDHDHPCTKIKCGLHEECSVTKSGAAVCLCPKECPHIFRRVCGTDGITYDNECELRKSACAEKVNVTFKHDGPCGPNQVCSTKVCHYGSMCVEHAGKAVCRCPQCTEEYNPVCGDNGITYQNDCKLKLENCQREENV 213 HSP 3 Score: 97.4413 bits (241), Expect = 1.036e-16 Identity = 107/469 (22.81%), Postives = 186/469 (39.66%), Query Frame = 0
Query: 552 CDPNPCHGGGTC---EEHDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSK-----GSLRTLNVGEL-IWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELS-RTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNV---GHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGE---KSMFPEKLEYLDFVSHGQLTFG 1003
C PC GGTC DG F C C + G+ C+ + S + + F G+S + + P L I + F T +GLIL G+ DF++L M+ +++ + + + LGSG + S + +WH++ R + G + +D +TG + G LN+ + +++G + + +V ++G+ GFTG ++ + ++ RT++ L + M +N+ PC PC NEG C EC C F G RC++ Q+ +++ P+ F G + S + S +TN+ L R G+ +L++ VS +G + + S + + + + + DG WH I RQ V +D E + FP + LD + G L G
Sbjct: 921 CASQPCLHGGTCVPVSSSDG-FYCQCAQSYEGSKCERE-SIPEVFVPQFTGDSYLEIPLPEIMTTSLSITVWFMTTKPDGLILLVT-QKPRGDGDFIAL---------MLENSS--------------LIYTFYLGSGVGRIESKKHIHLDKWHKVTISRNGRIGQMTIDAHAPITGEATFVNYVGPSTALNIDKRPLYLGGYN--LKSDVPAKLGSRTGFTGAIQRIIINGRTIVGLLATAVTM----RNISTYSGPPCNINPCMNEGVCIPMLHLAECRCTANFMGERCEKHFE-------------QVDKDQ-------PIRFNG-----TTFLSFLNEITRKVEGQQSNDFSIKFRTNVPDGILLLQ-----RSGDTVQSDYLSVAVS-NGYVELSFNLGRHSTDKLFIVRSKVKVDDGQWHHAVISRQHRIATVQVDNEEPVRDTFPISSQQLD--TDGILWIG 1324 HSP 4 Score: 82.8037 bits (203), Expect = 3.277e-12 Identity = 81/320 (25.31%), Postives = 119/320 (37.19%), Query Frame = 0
Query: 161 PKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDP----------------------CNEVTCKFGSYCS-----EGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQ------FRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPC 447
P+C+C VCG+DG TY + C L++ +C ++I + G C P C ++ C CS C+C +ECP + +C ++G TY N C L K CA + + F+ +V ++VC G C G +C RC + CG N + DC + C + S C D CG C+ A C + +CIC C + + VCG DG TY +EC++R SCR Q I I + C
Sbjct: 10 PECMCSFVCPSEINLVCGTDGRTYSNSCFLRQQACKSKKEIRVLNSGKCGPGLTILKGTIQDEYNRKLDHDHPCTKIKCGLHEECSVTKSGAAVCLCPKECPHIFRRVCGTDGITYDNECELRKSACAEKVNVTFKHDGPCGPNQVCSTKVCHY--------GSMCVEHAGKAVC-RCPQCTEEYNPV----CGDNGI-TYQNDCKLKLENCQREENVKESHKGACAD---------------------TCGNQRCHNYAICERVNNKPKCICPTSC---VQVDDPVCGNDGVTYANECEMRVESCRTQKFIGIGSKGTC 291
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Match: gi|321461745|gb|EFX72774.1| (hypothetical protein DAPPUDRAFT_308094 [Daphnia pulex]) HSP 1 Score: 409.838 bits (1052), Expect = 1.933e-120 Identity = 338/1213 (27.86%), Postives = 515/1213 (42.46%), Query Frame = 0
Query: 5 LSEDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKC---------ETCQDKKCGPHEECIEDTNGNPKCLC-QGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEP----------ICASNGKTYFNYCHLEKDNC-----ARQHDANFEQFRVVESRVCLE---------KVPVCP----------------------------------VDG--------CTCPHGVGGHLCDRCKTAFWAFTSFG-----CRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTL-----------STDSGCISVSSTKYE-RPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRI---TCGRSTF-------------------DMELK-------MTLISLMTDAPDQIYGQSKSPRQVLSDQKPSTGQFGETCLTDIDC-IVDNSHCSLNQCICDENHDYHADSRTCKS-----LSHKDTRPIHVSGLCDPNPCHGGGTCEEHDG-VFSCYCPPGFAGTLCQHDLSKSSISIA-----SFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYED-NVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVM-----------QRKNVID------------------CEENPCVRMPCSNEGSCSVKN-QGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSD--------PESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLV--------MELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGEKSMFPEKLEYLDFVSHGQLTFGPLTMRSLCLRHYVLKTWKNNRHSKTNPVDICK 1035
+ E +P C C+ C I PVCGSD +TY + CEL+ +C ++++I + + C + CS+ CP G C G+ C C C E++PVCG+D V Y N C LR SC+ +++ G C + C K+C + C D G +C+C + C+ EVCG+DGITY + C L++A+C + I G CD C V C+ + C +G C+C CP EP ICAS+G+TY CH+++ C + FR+ + LE K+ + P +DG C C GVGG CDRC + +W S G C C C+ GS DC+ TG C C+ G KC+ C DG L + G S S E C + C FGA C + G+ C+C +DC + VCG+DG +Y SEC LR +CRKQ ++ + PC I + G S D+ L ++L + + ++ YG S+ + + G GE+C+ D C ++ NS C C C + ++S++C + ++ P +S C PC GGGTC+ F C CP G+LC+ LS+S I +F G S + L A ++ ++++F++ +G++LY + + + D++SL II H VE R++LG+GP+VLRS + G WH +VA+RY +DG L +D D V +S G TL++ ++G V + R+GTG GF GC++ ++L R + + E + +R+N ID C ENPC + PC+N+G+C K + + C C PEFTG C+ + C +PC + C ++N FTC CP G G LC+ D PE + S S Y SF + NL S R G + + A + G + ++ R L S I + + + NWHRV + R+ + LD D + + GPL+ +L + ++ RH+ T P D+ K
Sbjct: 20 VDEKGMPQCLCKGPCPPIHR-----PVCGSDQLTYSSNCELERESCLQKRSIKLLYEGVCGSSEACSNFQCPTGGYCVETASGQPSCHCPLC---GGEWDPVCGTDGVTYTNPCRLRYESCRHNKSLSIVYKGLCNLIDDTKQADGCDKKRCDFYATCETDEMGRAECVCPKACAKMAITEVCGTDGITYRNECELKQAACRNQQFIVVASKGDCDLCKRVHCEHNARCEDGICVCPTNCPSPIEPSDPQSVGDVGICASDGRTYLTGCHMQRAACDHGLVLQTITIRLHPFRIPLTIQLLENATATSKVHKITIDPGVAHSILIRMLCGFLRLRFGAEYKLYWSSKISGSLDGACDPVTLQCRCLAGVGGRSCDRCLSGYWGIHSIGTNANGCEPCDCHRLGSIRADCEQMTGRCVCRANVVGLKCDRCQDGQLVDKVPGGCGGYGGNGGTGSGSDDALESESRHCSDLTCRFGAICQDLQGQAVCVCPMDCPVASSAEQTVCGSDGVSYGSECDLRLAACRKQLNVVMAYEGPCSDDIIHTSAGISPLMAAESEMAVSKATRHVQETDVALLDWGRRDIVSLSQVGSQKANEYYGFSQPVWRPTAASVHVHGYLGESCIDDSQCKLMSNSGCLFGVCKCSDGFAEDSNSKSCVANVATVSGMEELGPAKISS-CSSFPCLGGGTCQPLGMESFVCVCPAERTGSLCERALSQSDEEIVPAETPAFHGQSFVE-LKKMKAQDKFAMEIEFKSLLTDGILLYAQQRKDF-DADYISLAII-------------AGH----------VELRFNLGNGPIVLRSLQPIRLGHWHRVVAQRYRQDGWLRLDDDEDVATTSPGEHSTLDLDTNSFLGAVPSHLATARTYDRIGTGSGFVGCMRRVKLGRRSVIFRVPPEGRKVSRRGRQFDENKRRRNAIDGINKSTVSTVVKMNGITQCGENPCWKTPCANQGTCVWKTGENYTCVCRPEFTGLTCESALDLCASSPCGDGSTC--VSSKNRFTCKCPPGKKGFLCDQHDGMKHEILVPEFNIVHSPTSASSYVSVARSFQVSQNLDLEVWFLS---RSSDGMLAYSARDENGRGDFIWLALIGGRVQFRWDLGSGAGIVTSSERVTLF--NWHRVLVSRRGKEASIRLD-------------DGATSEGRSLGPLSELNLDIPLFI----GGLRHNLTLPRDVPK 1174 HSP 2 Score: 81.2629 bits (199), Expect = 9.286e-12 Identity = 85/309 (27.51%), Postives = 131/309 (42.39%), Query Frame = 0
Query: 142 CQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYCSE---GQCICR-QECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWA---FTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYRE-------AVCGTDGNTYRSECQLRQNSC 433
C C P EEC D G P+CLC+G VCGSD +TY S C L+R SC Q I + G C + C+ C G YC E GQ C C ++P+C ++G TY N C L ++C +H+ + +V +C DG CD+ + F+A G EC C + ++ A T VC ++CE+ + V ++K + + C C A C + C+C +C +P+ + +C +DG TY + C +++ +C
Sbjct: 7 CSKAGCEPDEECQVDEKGMPQCLCKGPCPPIHRPVCGSDQLTYSSNCELERESCLQKRSIKLLYEGVCGSSEACSNFQCPTGGYCVETASGQPSCHCPLCGGEWDPVCGTDGVTYTNPCRLRYESC--RHNKSLS---IVYKGLCNLIDDTKQADG-----------CDKKRCDFYATCETDEMGRAECVCPKACAKM----AITEVCGTDGITYRNECELKQAACRNQQ---FIVVASKGDCDL-CKRVHCEHNARCEDG----ICVCPTNCPSPIEPSDPQSVGDVGICASDGRTYLTGCHMQRAAC 287 HSP 3 Score: 67.3958 bits (163), Expect = 1.836e-7 Identity = 67/249 (26.91%), Postives = 105/249 (42.17%), Query Frame = 0
Query: 211 PCNEVTCKFGSYCSEG-----QCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCT---CPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDC---DAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATC-SNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPC 447
PC++ C+ C QC+C+ CP + P+C S+ TY + C LE+++C ++ +++ VC + C+ CP G + T+ G C C G E D T PC+ + C ++ C + TK + C + C+F ATC ++ GR C+C C VCGTDG TYR+EC+L+Q +CR Q I + + C
Sbjct: 6 PCSKAGCEPDEECQVDEKGMPQCLCKGPCPPIHRPVCGSDQLTYSSNCELERESCLQKR-----SIKLLYEGVCGSS------EACSNFQCPTG-----------GYCVETASGQPSCHCPLCGGEWDPVCGTDGVTYTNPCRLRYE--SCRHNKSLSIVYKGLCNLIDDTK--QADGCDKKRCDFYATCETDEMGRAECVCPKACAKMAITE--VCGTDGITYRNECELKQAACRNQQFIVVASKGDC 226
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Match: gi|1022773108|gb|KZS17220.1| (putative Agrin [Daphnia magna]) HSP 1 Score: 388.267 bits (996), Expect = 5.063e-111 Identity = 316/1138 (27.77%), Postives = 474/1138 (41.65%), Query Frame = 0
Query: 4 LLSEDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDAN--LCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLC-QGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSYEP----------ICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVD-------------------------GCTC-PHGVGGHLCD------------------RCKTAFWAFTSF-----GCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTL------------STDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRI---TCGRSTFDME-----------LKMTLISLM--------------TDAPDQIYGQSKSPRQVLSDQKPSTGQFGETCLTDIDC-IVDNSHCSLNQCICDENHDYHADSRTC-----KSLSHKDTRPIHVSGLCDPNPCHGGGTCEEHDG-VFSCYCPPGFAGTLCQHDLSKSSISIA-----SFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYED-NVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKN-----------------------------VIDCEENPCVRMPCSNEGSCSVKN-QGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSD--------PESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLV--------MELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLD 979
+L ++ P C C + C + PVCGSD +Y + C L + AC Q+ + + CE + C+ CP G C G+ C C C E++PVCGSD V Y N C LR SC+ +++ G C C K+C + C D G +C+C + C + EVCG+DGITY + C L+ A+C + I G CD C +V C++ + C +G C+C CP EP ICAS+G+TY CH+++ C H +V+ + C P P D C C G CD RC + +W S GC C C+ GS DC+ TG C C+ G KC+ C DG L + C + C FGA C + G+ C+C +DC + VCG+DG +Y SEC LR +CRKQ ++ + PC I + G S E ++ T ++L+ + ++ YG S+ + + G GE+C D C V NS C C C + +D ++C + ++ P+ +S C PC GGGTC+ F C CP G+LC+ LS+S I +F G S + L A ++ ++++F++ +G++LY + + + D++SL II H VE R++LG+GPVVLRS + GQWH +VA+RY +DG L +D D V +S G TL++ ++G V + R+GT GF GC++ +++ R + + E + R+N + C ENPC + PC+NEG+C K F C C P+FTG C+ + C +PC + C ++N FTC CP G G LC+ D PE + S Y SF + NL S R G + + A + G + ++ R L S I + + + NWHRV + R+ + LD
Sbjct: 154 VLDSERKPHCRCGDTCPSDFQ-----PVCGSDGRSYSSQCHLMQEACRSQRQLRILYKGLCESGSSEACNHFRCPTGGYCVETASGQPSCHCPPC---GGEWDPVCGSDGVTYTNPCRLRYESCRNNKSLSIVYKGLCNGCDKKRCDFYATCETDELGRAECVCPKACGKLANAEVCGTDGITYRNECELKLAACRNQQFIVVASKGDCDLCKKVRCEYDARCEDGICVCPTNCPSPIEPPDSERVGDLGICASDGRTYLTECHMQRAAC--DHGL---VLKVLHTGPC----PTPPADLILAASDYHDSSSSMSSPSHNLPPRKCHCNKQGSADGACDPVTLQCRCLAGVGGRSCDRCLSGYWGIHSVGKNANGCEPCDCHRLGSIRADCEQMTGRCVCRANVVGLKCDRCHDGQLVDKVPGGCGGYGGSGGAGSGSDDALESESRHCSDLTCRFGAICQDLQGQAVCVCPMDCPVASSAEQTVCGSDGVSYGSECDLRLAACRKQLNVVMAYEGPCSDDIINTSAGMSPLVAESETAVSKATRHVQETDVALLDWGRRDIVSLSQVGSQKANEYYGFSQPVWRPTASSVHVRGFLGESCTDDSQCKSVANSGCLFGVCGCSDGFAEDSDRKSCVANIASTSGSEEYGPVRLSS-CSSFPCLGGGTCQPLGMESFVCLCPAERTGSLCERALSQSDEEIVPAETPAFHGQSFVE-LKKMKAQDKFAMEIEFKSLLTDGILLYAQQRKDF-DADYISLAII-------------AGH----------VELRFNLGNGPVVLRSLQPIRLGQWHRVVAQRYRQDGWLRLDDDEDVATTSPGEHSTLDLDTNSFLGAVPSHLATARTYDRIGTASGFVGCMRRVKIGRRSVIFRVPPEGRKVSRRNRQFEENKRRGNAIGGVSKSSISTVVKMNGITQCGENPCWKTPCANEGTCVWKTGDNFTCICRPQFTGLTCESALDLCESSPCADGSTCTSSKNR--FTCKCPPGRKGFLCDQHDGMKHEILVPEFNIVHSSTPASSYVSVARSFQVSQNLDLEVWFLS---RSSDGMLAYSARDENGRGDFIWLALIGGRVQFRWDLGSGAGIVTSPDRVKLF--NWHRVLVSRRGKEASIRLD 1241 HSP 2 Score: 120.168 bits (300), Expect = 1.324e-23 Identity = 86/289 (29.76%), Postives = 140/289 (48.44%), Query Frame = 0
Query: 13 CACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNIT--------QISDQQCEDANLCSSDPCP-----FNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCET-----CQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYC-----SEGQCICRQECPD-SYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESR 277
C C C ++ G+ PVCG+D Y N CEL +++C + I +++ QQ + +L SDPC VC + E + C+C + +++PVCGSD Y ++C L + +C+ + ++ + G CE+ C +C C+E +G P C C C + + VCGSDG+TY + C L+ SC N+ + + G C+ C++ C F + C +C+C + C + +C ++G TY N C L+ C Q QF VV S+
Sbjct: 73 CQCPTKCSSYGDSVGSRPVCGADGKDYPNTCELHKSSCLANRRIDVKFQGACGRVAIQQ-DICSLFLSDPCASIECLSTSVCVLDSERKPHCRCGDTCPS--DFQPVCGSDGRSYSSQCHLMQEACRSQRQLRILYKGLCESGSSEACNHFRCPTGGYCVETASGQPSCHCPPCGGE-WDPVCGSDGVTYTNPCRLRYESCRNNKSLSIVYKGLCNGCDKKRCDFYATCETDELGRAECVCPKACGKLANAEVCGTDGITYRNECELKLAACRNQ------QFIVVASK 351 HSP 3 Score: 103.99 bits (258), Expect = 1.071e-18 Identity = 108/423 (25.53%), Postives = 168/423 (39.72%), Query Frame = 0
Query: 70 CSSDPCPFNGVC-ARNYEGRFQCQCQ---ECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKC---------------ETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC-----DPCNEVTCKFGSYCSE---GQCICR-QECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGC-ISVSSTKYERPIP---------CGESECNFGATCSNTGGRLRCICEIDCRNPLYYREA-------VCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPC 447
C+ CP C A CQC Y + PVCG+D YPN CEL KSSC I VK G C + C +C C+ D+ P C C + VCGSDG +Y S CHL + +C + + G C + CN C G YC E GQ C C ++P+C S+G TY N C L ++C N + +V +C + CD+ + F+A C +E G +C VCP K + E+C ++ + C + +++ + ++ I C + C + A C + C+C +C +P+ ++ +C +DG TY +EC +++ +C + + + PC
Sbjct: 51 CADLKCPPGAKCIAAADASSATCQCPTKCSSYGDSVGSRPVCGADGKDYPNTCELHKSSCLANRRIDVKFQGACGRVAIQQDICSLFLSDPCASIECLSTSVCVLDSERKPHCRCGDTCPSDFQPVCGSDGRSYSSQCHLMQEACRSQRQLRILYKGLCESGSSEACNHFRCPTGGYCVETASGQPSCHCPPCGGEWDPVCGSDGVTYTNPCRLRYESC-----RNNKSLSIVYKGLC--------------------NGCDKKRCDFYA-------TCETDELG--RAEC-----VCP-KACGKLANAEVCGTDGITYRNECELKLAACRNQQFIVVASKGDCDLCKKVRCEYDARCEDG----ICVCPTNCPSPIEPPDSERVGDLGICASDGRTYLTECHMQRAACDHGLVLKVLHTGPC 429 HSP 4 Score: 95.1301 bits (235), Expect = 5.734e-16 Identity = 96/341 (28.15%), Postives = 138/341 (40.47%), Query Frame = 0
Query: 138 KCETCQDKKCGPHEECIEDTNGNP-------KCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---------------DPCNEVTCKFGSYC-----SEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDC---DAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATC-SNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPC 447
K + C D KC P +CI + + KC G + S VCG+DG Y + C L ++SC N I + GAC DPC + C S C + C C CP ++P+C S+G++Y + CHL ++ C Q Q R++ +C E + CP G + T+ G C C G E D T PC+ + E C + S+S C + C+F ATC ++ GR C+C C VCGTDG TYR+EC+L+ +CR Q I + + C
Sbjct: 47 KPDPCADLKCPPGAKCIAAADASSATCQCPTKCSSYG-DSVGSRPVCGADGKDYPNTCELHKSSCLANRRIDVKFQGACGRVAIQQDICSLFLSDPCASIECLSTSVCVLDSERKPHCRCGDTCPSDFQPVCGSDGRSYSSQCHLMQEACRSQR-----QLRILYKGLC-ESGSSEACNHFRCPTG-----------GYCVETASGQPSCHCPPCGGEWDPVCGSDGVTYTNPCRLRY-----ESCRNNK--------SLSIVYKGLCNGCDKKRCDFYATCETDELGRAECVCPKACGK--LANAEVCGTDGITYRNECELKLAACRNQQFIVVASKGDC 354
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Match: gi|1008426663|ref|XP_015835893.1| (PREDICTED: agrin isoform X12 [Tribolium castaneum]) HSP 1 Score: 384.415 bits (986), Expect = 5.595e-109 Identity = 326/1141 (28.57%), Postives = 484/1141 (42.42%), Query Frame = 0
Query: 30 PVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKP---SEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSY-EPICASNGKTYFNYCHLEKDNCARQHDAN----------FEQFRVVESRVCLEKVPVCPVDG---------------------CTCPHGVGGHL---------------------------CDRCKTAFWAFTSFGCRE-CGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTL------STDSGCISVSSTKY-----------------------------------------------ERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQG-----------RITCGRST----FDMELKMTLISLMTDAPDQIYGQSKS---PRQVLS-----DQK------------------------------PSTGQFGETCLTDI-----DCIVDNSHCSLNQCICDENHDYHADSRTCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVD-GDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCS-VKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCE------------DSDPESSWERYRKSTSKYDRQKVSFGLQTN-LSKAAD-LFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLD 979
PVC D T+ + CELK AC + I C + CS C C + G C+C C E+EPVCGSD + Y NEC+LR +C+ ITV G C C+ KKC + C D+ +C+C P VCG+DG+TY + C L+ SC + I + G CD C V CK+G C G+C+C C S EP+CASN T+ N C L+K C + ++ E+F V S L ++ V P +G CP G H C KTA GC CGCN+ GS S CD +T C C+ G G+KC+ C G S GCI + + P C E C FGATC GG C C +C + VCG+DG TY S C+LRQ +CR Q DI + F C+ R T + T + L + L+ P YG S S P Q +S D K P+T + L D+ DC++ SHC C C NH +D + C + D P C +PCH G TC + F+C C F G+LC+ ++ + +F G S + L P A ++L I+++F+T + +GL+LYN+ + G DF+SL I++ FVEF+Y+LG+G V++RS ++ G +H +V KRYH+DG+L++D G+ V G +KG+L+ L++ E ++G + + Y V + +GT GF GC++ L++ R I L ++ V+ + V +C ++PC PC+N+ C+ + + + CEC P + G C++ C NPCK C + +++ C CP G G CE +PE S Y + + + + +F ++ + KAA+ L +L+ G ++L +++ ++ R L S IA+L T ++ G WH I R I+ LD
Sbjct: 470 PVCSKDGRTFPSECELKRTACLTRTTIEISYSGVCGEKGPCSEYQCQNGATCVERF-GVAHCECPVC---PAEFEPVCGSDGISYGNECKLRLEACKHPRNITVLYDGPCNGCETKKCDFYSVCERDSATEGRCVCPKSCTDPELNDGTVCGTDGVTYANECELRMTSCKMKQFILVAYKGNCDLCQGVECKYGGRCEAGKCVCPTNCEGSGDEPVCASNMMTFPNECELQKAMCLQPTNSPPLSVVFYGDCREKFPVAGS---LSRMEVKPCNGEKPLTDPLTGKELDCGSGPKRQDCPSGSYCHQTTRFSRCCPKDQGLLEQKSCEDSWHGCCPDGKTAAQGPNHAGCPSLCGCNKLGSYSDTCDPETQQCRCRPGVGGAKCDRCEPGYWGLPKISSGYHGCIPCGCSTFGSVRDDCEQMTGRCVCNPGVQGQKCTVCTSHDKVLGPNGCVPADLTTSPPTTCKELTCYFGATCVERGGFAICECHTECPQE-NDAQVVCGSDGQTYASACELRQVACRTQKDIVVQAFGTCKDDMFPSTDWPIRRYTPLQFTQPDDSNSPLSKSTRHLLVPDPRYYYGSSGSIMRPVQGVSYPAETDNKAAFEINVSATGPNAIYNTRGGNYAPAYRPTPATVRVITALLGDLCSDNSDCMIMYSHCVSGACTCLPNHSESSDRQECIA----DPNPTDEYRACSSSPCHHGSTCVDLPAATFTCVCETNFTGSLCETEVIHKQYNTPAFHGRSYVK-LKPLKAYHKLSIEVEFKTHSHDGLLLYNQQKPD-GLGDFVSLAIVN-----------------------GFVEFKYNLGNGHVLIRSVDKIQLGVFHRVVIKRYHRDGILKLDEGEDVAGQAKGTLKALDLLEDTYVGFLPSNYT-RVYENIGTAKGFRGCIRKLKIGRRPIELHVNRDDWVLGAEAVSECGDSPCSAAPCANDAKCALITPETYRCECGPHYRGEFCEKPIEPCRSNPCKFGSTC-TPLSPDAYICECPPGRTGTRCEMVCSVSEDKYMGIVNPEFSGSSYIR-LPRLEGVRKTFSIEVYFMPKAANGLILYNGQLKNGRGDFISLNLARGH---LQFRFNLGSG--IANLTTKETVNIGKWHWARIFRDGREGILQLD 1565 HSP 2 Score: 160.229 bits (404), Expect = 6.498e-36 Identity = 137/441 (31.07%), Postives = 195/441 (44.22%), Query Frame = 0
Query: 31 VCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGR-FQCQCQE---CYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC----DPCNEVTCKFGSYCSEGQC-ICRQECPDSYEPI----CASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPV---DGCTCPH--GVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCS-NTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAIT---NFAPCQG 449
VCG+D TY N C+L+ A+C + N D +CE + C C F C + +GR C C + Y +H PVCGSD + Y N+CEL+K++C IT+K GKC+ C +C E C D + NP C C VCGSDG TY + C L++ +C +++ + G C +PC V C G C+ + I R +CP S EPI C+ +G+T+ + C L++ C + + S VC EK P +G TC GV C C F CG + S +C + C R T+ D C C +C+F + C ++ RC+C C +P VCGTDG TY +EC+LR SC+ + I + N CQG
Sbjct: 248 VCGTDGKTYANRCQLRVASCKARLNTRVKHDGECEQNDPCRDKQCGFGARCVVSPDGRNASCVCPDKCPSYGDHTTSRPVCGSDGIDYRNQCELQKAACTSNTNITIKFLGKCDPCAGVECAEPEVCQLDEHRNPVCRCGDSCPLEFTPVCGSDGKTYSNECTLRQEACRARKNLHIIYRGKCSSGINPCMSVRCTLGEECAINKFGIARCQCPPSCEPIMRPVCSKDGRTFPSECELKRTACLTR-----TTIEISYSGVCGEKGPCSEYQCQNGATCVERFGVAHCECPVCPAEFEPV-------CGSDGI-SYGNECKLRLEACKHPRNI-----------TVLYDGPCNG-----------CETKKCDFYSVCERDSATEGRCVCPKSCTDPELNDGTVCGTDGVTYANECELRMTSCKMKQFILVAYKGNCDLCQG 653 HSP 3 Score: 146.362 bits (368), Expect = 1.175e-31 Identity = 124/395 (31.39%), Postives = 176/395 (44.56%), Query Frame = 0
Query: 70 CSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCE---TCQDKKCGPHEECIEDTNG-NPKCLCQG-----CSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQ-----CICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDA-QTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQG 449
C C + C +GR CQC ++ + VCG+D Y N C+LR +SC+ ++ VK G+CE C+DK+CG C+ +G N C+C + S VCGSDGI Y + C LQ+A+CT N +I + G CDPC V C C + C C CP + P+C S+GKTY N C L ++ C + + ++ C + C CT L + C A FG C C C+ VC S+CE+ L+ + IS S E+ PC E +C GATC G C C + P + E VCG+DG +Y +EC+LR +C+ +I + PC G
Sbjct: 214 CEKTFCAWGAQCITGPDGRAMCQCPT--HCKQKVDLVCGTDGKTYANRCQLRVASCKARLNTRVKHDGECEQNDPCRDKQCGFGARCVVSPDGRNASCVCPDKCPSYGDHTTSRPVCGSDGIDYRNQCELQKAACTSNTNITIKFLGKCDPCAGVECAEPEVCQLDEHRNPVCRCGDSCPLEFTPVCGSDGKTYSNECTLRQEACRAR-----KNLHIIYRGKCSSGINPCMSVRCT--------LGEEC-----AINKFGIARCQC------PPSCEPIMRPVCSKDGRTFPSECELKRTACLTRTTIEISYSGVCGEKG-PCSEYQCQNGATCVERFGVAHCECPV---CPAEF-EPVCGSDGISYGNECKLRLEACKHPRNITVLYDGPCNG 577 HSP 4 Score: 126.716 bits (317), Expect = 1.356e-25 Identity = 127/480 (26.46%), Postives = 196/480 (40.83%), Query Frame = 0
Query: 13 CACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCET----CQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYCSE----GQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFS------GSKCEICPDGTLSTDSGCISVSSTKYERPI---------PCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTD---IAITNFAPCQGRITCGRSTFDMELK 463
C C + C + + PVCGSD + YRN CEL++AAC NIT +C+ C+ C VC + C+C + E+ PVCGSD Y NEC LR+ +C+ + + + GKC + C +C EEC + G +C C VC DG T+ S C L+R +C I + G C PC+E C+ G+ C E C C CP +EP+C S+G +Y N C L + C +H N T + GC C+ + +D A G C C + + G+ C DG + + ++S K ++ I C EC +G C +C+C +C E VC ++ T+ +EC+L++ C + T+ +++ + C+ + S ME+K
Sbjct: 309 CVCPDKCPSYGDHTTSRPVCGSDGIDYRNQCELQKAACTSNTNITIKFLGKCDP---CAGVECAEPEVCQLDEHRNPVCRCGDSCP--LEFTPVCGSDGKTYSNECTLRQEACRARKNLHIIYRGKCSSGINPCMSVRCTLGEECAINKFGIARCQCPPSCEPIMRPVCSKDGRTFPSECELKRTACLTRTTIEISYSGVCGEKGPCSEYQCQNGATCVERFGVAHCEC-PVCPAEFEPVCGSDGISYGNECKLRLEAC--KHPRNI--------------------------------------TVLYDGPCNGCETKKCDFYSVCERD-SATEGRCVCPKSCTDPELNDGTVCGT--DGVTYANECELRMTSCKMKQFILVAYKGNCDLCQGVECKYGGRCEAG----KCVCPTNCEGS--GDEPVCASNMMTFPNECELQKAMCLQPTNSPPLSVVFYGDCREKFPVAGSLSRMEVK 733 HSP 5 Score: 107.842 bits (268), Expect = 7.019e-20 Identity = 115/464 (24.78%), Postives = 180/464 (38.79%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQHDLSKS-----SISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRVT-GSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEEN---------PCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERY--RKSTSKYDRQKVSFGL-QTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQL------------VMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGEKSM 984
C NPC G TC + C CPPG GT C+ S S I F G+S + + I++ F A NGLILYN G L+ G DF+SL + R H ++FR++LGSG L + V G+WH R ++G+L++D + G S L LN+ +IG++ D V + G G+ G ++ + L+ + + S + P N C N P + PC N G C + F C+CP G+ C+ + P N RN + + + E+ D ++ E + YD ++ F T K ++LR L L S+S + E+ L + V ++ + I DG WH + + R+ + +DGE +
Sbjct: 1417 CRSNPCKFGSTCTPLSPDAYICECPPGRTGTRCEMVCSVSEDKYMGIVNPEFSGSSYIRLPRLEGVRKTFSIEVYFMPKAANGLILYN-GQLKNGRGDFISLNL-------------ARGH----------LQFRFNLGSGIANLTTKETVNIGKWHWARIFRDGREGILQLDNSSIVRGYSGTPLTELNLELPFYIGSLSEW--DEVHRLSGASKGYKGVIQRILLNGKPLPI-SAKLPDCSFDSNRNGCAFNVGSYDGLPCPISKNPCLNNGLCVPELDQFICKCPSNTKGKYCEISQEE-TPAIKFNGATFLQFRNRGYRSHNLTISDEYPDNEEDDIDNIEEDVVDNNTYDAYDDEEYYFDFYDTARRKPERGNRYEIKLRTFASDALLLWRSKSRSIREDYFSIAVVNGYPEISYNLGKQDVFWAIRSKTKIDDGKWHTIQVRRRKRVGFISIDGEPPI 1852
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Match: gi|1069821080|ref|XP_018333496.1| (PREDICTED: agrin-like isoform X3 [Agrilus planipennis]) HSP 1 Score: 375.17 bits (962), Expect = 8.154e-107 Identity = 311/1072 (29.01%), Postives = 466/1072 (43.47%), Query Frame = 0
Query: 30 PVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQC-QECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAE---HYGACD--------------PCN---------------------EVTCKFGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQF----RVVE-SRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFT-----SFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEIC--PDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCG---------------------------RSTFDMELKM------TLISLMTDAPDQIYGQSKSPRQVLSDQKPSTGQ---------------------------FGETCLTDIDCIVDNSHCSLNQCICDENHDYHADSRTCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEEHDGV-FSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDR-VTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSC-SVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSD-------PESSWERYRKSTSKYDRQKVSFGLQ-TNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLD 979
PVCGSD +TY N C+LK +C +QQ I + C+ C S C F +C N + QC C C + PVCG+D V Y NECE+R SC+ + I+V G C +C ++C + + N C+G +P VC S T+ + C Q+A+C ++ D+ YG C PCN C GSYC + R CP + S +++ C EK ++A R+ S+ C CP C GVGG CD C FW S GC C C+ FGS DC+ TG C CK G G+KC C PD L + GC+ + P C E C+FGA+C GG C C +C N + VCG+D TY S CQL+ +CR Q DI + F PC+ G R +LK+ + I + PD Y + + + +D PS+G G+ C D DC++ +S C C C + +D + C S + C +PC G C + F+C C F G LC +SK++ + SF G + + L P A +L I+++F+ + NG++LYN+ E G+ DF+S+ +++ F+EFRY+LG+GP V++S ++ ++H +V KRY +DG L+++ D + G SKG+ L++ E ++GNV T Y+ V + +GT G GC++ L++ R + L + + V+ + + +C +PC PC N+G C +V ++ + CEC P F G C+ + C NPCK +C EN F C CP G GR C+ D PE + Y K + K +F L+ LSK+ + + + N G + V+ G V + R L S IA++ ++ PI WHR+ +LR + LD
Sbjct: 118 PVCGSDGITYGNICKLKLESCKQQQEIRVMYTAPCDG---CESRKCDFYSMCVPNEDIGTQCVCPTSCTDAKLLKGPVCGNDGVTYVNECEMRMISCKTQQLISVSYRGPCNSCNGEECPIDGDDCKSGNCQCPVKCEGVREEP---VCASSNKTFINECEFQKANCERSPDLLPLTILFYGNCSERFTAASTLSSEVKPCNGEKPVVDPFTNEEFDCGNNANRRDCPTGSYCHITLTVARC-CPIEVKKESNSCKDSWYGCCPDEKTPAQGPNNAGCPSVCRCNRLGSYSKSCDPTTLQCP-----CKPGVGGQKCDSCLPGFWGLQHIDKGSVGCIPCACSAFGSVRVDCEQMTGRCVCKPGVQGNKCTFCSNPDKVLGPN-GCVP-ADVAAPPPTTCKELTCHFGASCVEQGGTAFCDCYNEC-NEKTDNQIVCGSDDQTYTSSCQLQLLACRLQKDIVVQAFGPCKDDTLPGTELPFRRYTPLQFTQSDESNSPLSKSTRHLMVPDLKVYYERAGSYIPPRSSYPDTQYISNSA--SIGNDLYPSSGTGIFESRGEHIAAAYRPTPATVRVITALLGDLCSNDSDCLILHSSCVGGACTCLPGYAESSDRQACNEASTTEE-----FRACFSSPCAEGSRCVDLPAASFACICSVNFTGQLCDIPISKTNFDVPSFDGQAYIR-LKPLKAYYKLSIEIEFKAYTNNGILLYNQ-QRENGQGDFVSIAVVNG-----------------------FIEFRYNLGNGPTVIKSLDKIQLNRYHTVVVKRYRRDGFLKLNEDESIVGQSKGTWEALDLAEETFVGNVITTYK-KVYENIGTNQGLRGCIRRLKIGRRGVKLNGDVDDFVLGVQGIQECGSDPCSGSPCQNDGICQAVDSELYRCECGPSFIGDFCENVIDPCLSNPCKGGSECFSLDTEN-FMCQCPAGRKGRTCDLVDTNWSVKIPEFNGSSYLKLPTLKGVMK-TFSLEVVFLSKSLNGLILYNGQKKKNKGDF-ISVNLVGGFV-QFRFNLGSG--IANITSSKPIEINKWHRLRVLRDGREGFLQLD 1135 HSP 2 Score: 120.553 bits (301), Expect = 8.147e-24 Identity = 114/459 (24.84%), Postives = 180/459 (39.22%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEEHDGV-FSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRV-TGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEEN-------PCV--RMPCSNEGSCSVKNQGFECECPPEFTGRRCQ-----------------RKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDR-QKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGE 981
C NPC GG C D F C CP G G C + S+ I F G+S + + + +++ F + + NGLILYN G + + DF+S+ ++ FV+FR++LGSG + S+ + +WH L R ++G L++D + G S SL LN+ +IG+V++ E + L GF G L+ L ++ I + + +N+ C PCV + PC N G C + C+CPP F G+ CQ + RN + N + A E FG +P S+ + K +R K F L+T S L+ R + +A+ EL L + ++ T I DG WH + + R+ + +D E
Sbjct: 992 CLSNPCKGGSECFSLDTENFMCQCPAGRKGRTCDLVDTNWSVKIPEFNGSSYLKLPTLKGVMKTFSLEVVFLSKSLNGLILYN-GQKKKNKGDFISVNLVGG-----------------------FVQFRFNLGSGIANITSSKPIEINKWHRLRVLRDGREGFLQLDDSSIEKGWSGASLTDLNLELPFFIGSVESWREIHRLSEANK--GFKGALQRLVVNGESIPISANFPECSSLPENITTCSSGVGLYDGPPCVSSKTPCHNNGLCIPNLDDYICKCPPSFEGKHCQIAKEEGISVKFTGETFLQYRNRGYKNETFYDYEEDYAEQEYLEVLEDYELDFGY-----EPTESYSDFDFPWKKAERGNKYIFKLRTFASDGLLLWRSKTRSIQEDYFSVAIVNGYP-----ELSYNLGKQKAFWAIRTKRRIDDGEWHTLEVRRRKKMGFISVDHE 1414 HSP 3 Score: 80.1073 bits (196), Expect = 2.447e-11 Identity = 93/353 (26.35%), Postives = 145/353 (41.08%), Query Frame = 0
Query: 137 GKCET----CQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYCSEGQCICRQE---CPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICP----DGTLSTDSGCISVSSTKYERPI------PCGESECNFGATCSNTGGRLR---CICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTD---IAITNFAPCQGRITCGRSTFDMELK 463
GKC T C++ +C + EC D G+ C+C + VCG+D TY SLC L+R +CT N + + G C PC+ C+FG+ C E I + C ++P+C S+G TY N C L+ ++C +Q ++ RV+ + P DGC R+C ++D Q C C + +K P DG + + + S K ++ I PC + CN G C G + C C + C E VC + T+ +EC+ ++ +C + D + I + C R T ST E+K
Sbjct: 5 GKCTTDENPCEELECKHYRECSIDKRGHATCVCPPPCEMKMKPVCGNDDRTYSSLCELKRTACTFNLTLEPAYQGVCGEMGPCSHHVCRFGAVCQEKNGIAACQCFTCSSEFQPVCGSDGITYGNICKLKLESCKQQ-----QEIRVMYT---------APCDGCE------------------------SRKCDFYSMCVPNEDIGTQ---CVCPTSCTDAKLLKGPVCGNDGVTYVNECEMRMISCKTQQLISVSYRGPC--NSCN-GEECPIDGDDCKSGNCQCPVKCEG--VREEPVCASSNKTFINECEFQKANCERSPDLLPLTILFYGNCSERFTAA-STLSSEVK 310
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Match: gi|1274113092|ref|XP_022821464.1| (agrin-like isoform X5 [Spodoptera litura]) HSP 1 Score: 377.481 bits (968), Expect = 1.181e-106 Identity = 320/1145 (27.95%), Postives = 485/1145 (42.36%), Query Frame = 0
Query: 5 LSEDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKV---EITVKKYGKCETCQDKKCGPHEECIEDTN-GNPKCLC-QGCSN-KPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSY-EPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPV---------------------------------------CPVDGCTCP--------------------HGVGGHLCDRCKTAFWAFTSF-----GCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEIC--------PDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRSTFDMELKMTLISLMTDA-----------PDQIYGQSKSPRQ--------VLSDQK-----PSTGQ------FGETCLTDIDC-IVDNSHCSLNQCICDENHDYHADSRTCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEEHDG-VFSCYCPPGFAG----TLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDG-DRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQ-SEQEPMVMQRKNVIDCEENPCVRMPCSNEGSC-SVKNQGFE--CECPPEFTGRRCQRKRNHCHPNPCK-NKGQCQIARNENSFTCSC----PVGFFGRLCED--SDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQL------------VMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGEKSMFPEKLEYLDFVSHGQLTFGPLTMRSL 1010
L V C C C+ + PVCGSD TY + C L A+C +++ C N C+ CP+ G C G QC C C PVC SD Y +EC++R +CQ+ V E+ V G C+TC D C +C+ D + G P C C C+ + S+ VCGSDG TY S C L +C + D+ + G C C V C FG++C+ G+C+C +C + EP+C S +T+ N C L+K C A + + L VP CP+ TC GVGG CDRC+ +W GC CGC+ FGS +DC+ TG C C+ G G KC +C P+G ++G + S C E C FGA C+ G C C + VCG+DG TY SEC L+ +CR Q DI + F PC+ G + + D PD+ Y + R+ +LS QK + G G+ C D DC + + C+ + C+C H + C ++T + + C PC+ GTC + G ++C C + G +L + L + I SF G S + L P A ++L I ++F+ F++NG+ILYN+ + G DF+SL +++ ++EFRY+LG+G ++L S ++ ++H++ AKRYH+DG+L VD + V G S G L+ L++ E +IG+V T + V +GT F GC+K L + R + + + +V+ +NV +C+ NPC+ MPC N +C S+ E C CP F G C + + C NPC ++G + C C +G G C + + + +W TS + + L KA F I + + L QS + ++ R L S IA++ + +PI+ G WHR + R + LD + ++ ++H +LT PL + SL
Sbjct: 512 LDARGVAVCGCGAECEAVVR-----PVCGSDGRTYDSRCHLDRASCLDNRDVRVAYTGPCGVENPCARASCPWGGACVAR-GGAAQCACPVC---DATLTPVCASDRNTYGSECKMRMHACQEGVPDGELKVLYNGTCQTCSDIVCKGGGDCVVDADSGRPICRCNHNCTEAQESDVVCGSDGQTYPSQCELDSTACREQTDLRLAYKGDCALCEGVQCAFGAHCAAGECVCPTDCTGAAREPVCGSTMQTFPNECELQKAACRLPPPATLHVIFYGDCKDRLAVVPPIAMNDSRQFEEKKISHCSETSYGCCSDGSTAASGPNEEGCPITSSTCGCNRLGSVSDRCDEDGQCVCRPGVGGLKCDRCEPGYWGLPRIGSGHTGCIPCGCSAFGSVREDCEQMTGRCVCRTGVQGQKCTVCADHRRRLGPNGCSDPETGGVVSS---------CAELSCYFGAVCTERTGGALCECAAAPCSDSDTNMLVCGSDGKTYESECHLKLQACRTQEDIVVQAFGPCKLGEVAGTAGPPRPSSPIQFTQQDDGAASKSTRHLLNPDKYYNKYDWTRKETPSDFDSILSGQKFKATTATVGSGAVGALLGDLCADDGDCAALPGAECARDACVCRAGHVPTLHRKACVEQIPQETTEEYSA--CLSEPCYNLGTCIDLPGSTYTCLCAGPYTGLNCESLAKDGLLATYIETPSFDGTSYIR-LKPLKAYHKLNIDIEFKAFSENGVILYNQQKPD-GTGDFVSLALVN-----------------------GYLEFRYNLGNGVLILTSLEKITLNEYHKVSAKRYHRDGILSVDDMEDVVGQSSGHLKALDLNEDAYIGSVPTNFS-RVFDNIGTRHSFIGCVKYLRIIRHQVTKKLGRPDSLVVAIENVQECQSNPCMSMPCKNGATCKSIDGSATEYTCSCPIGFQGANCDERLDPCESNPCGYDEGLLCDIGTDGGHVCRCLFGGEIGSNGNTCNNDVNVVQETWSPQFNGTSYIELPPLE-----GLGKA---FRIEIWFLTNRFSGMLLYTGQSNKAKGDFIAINLVNGYLQFRYNLGSG--IANITSPVPITKGRWHRARVSRSGRHGSLQLDHHPT---QRGHSFPPLTHLELTL-PLFIGSL 1596 HSP 2 Score: 147.517 bits (371), Expect = 4.918e-32 Identity = 133/465 (28.60%), Postives = 195/465 (41.94%), Query Frame = 0
Query: 8 DQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSN---KPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC----DPCNEVTCKFGSYCS-----EGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQ-TGVCPCKRGFSGSKCEI--------CPDGTLST--DSGCISVSSTKYERPIPCGESECNFGATC--SNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPC 447
D CAC C PVC + TYRN C L++ AC ++ N+ + +C+ + C+ CP C Y G+ +C+C + + PVCG+D +Y + C L K +C + IT+K +GKC+ C + +C C + P C C + +P VCGSD Y S C L+R SC + + + G C PC+ V C +C+ C C EC P+C S+G+TY + CHL++ +C D RV + C + P +CP G G +C C CDA T VC R GS+C++ PDG L + C + C + C G C GR C C +C + VCG+DG TY S+C+L +CR+QTD+ + C
Sbjct: 297 DGRAHCACPTTCPT-----SPAPVCSTAGRTYRNHCYLRKEACERRLNLRVKHEGECDAGDPCTGVTCPAGARCVAAY-GQAECRCPRSCQRRK---PVCGTDGREYSSVCHLDKHACDNQFNITIKYHGKCDPCSEHECADGGVCQLNEARAPVCRCGPPCDLLVRPGSAVCGSDYKDYPSECALRRESCRTRQQLTIAYRGECASAQHPCDSVKCGSREHCTLDARGVAVCGCGAECEAVVRPVCGSDGRTYDSRCHLDRASCLDNRD-----VRVAYTGPCGVENPCARA---SCPWGGA------------CVARGGAAQCAC-------PVCDATLTPVCASDRNTYGSECKMRMHACQEGVPDGELKVLYNGTCQT-----------CSDIVCKGGGDCVVDADSGRPICRCNHNCTE-AQESDVVCGSDGQTYPSQCELDSTACREQTDLRLAYKGDC 713 HSP 3 Score: 140.584 bits (353), Expect = 6.347e-30 Identity = 134/442 (30.32%), Postives = 186/442 (42.08%), Query Frame = 0
Query: 30 PVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQC-QECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCET----CQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYC----SEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGV--GGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESE---CNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCR--KQTDIAITNFAPCQGRIT 452
PVCG+D Y + C L + AC Q NIT +C+ CS C GVC N C+C C R VCGSD YP+EC LR+ SC+ + ++T+ G+C + C KCG E C D G C C VCGSDG TY S CHL RASC N D+ + G C +PC +C +G C QC C C + P+CAS+ TY + C + C Q + +V+ + C TC V GG C G C CN +E+++ D VC S+CE+ + C + + C E C FGA C+ C+C DC RE VCG+ T+ +EC+L++ +CR + + + C+ R+
Sbjct: 385 PVCGTDGREYSSVCHLDKHACDNQFNITIKYHGKCDP---CSEHECADGGVCQLNEARAPVCRCGPPCDLLVRPGSAVCGSDYKDYPSECALRRESCRTRQQLTIAYRGECASAQHPCDSVKCGSREHCTLDARGVAVCGCGAECEAVVRPVCGSDGRTYDSRCHLDRASCLDNRDVRVAYTGPCGVENPCARASCPWGGACVARGGAAQCAC-PVCDATLTPVCASDRNTYGSECKMRMHAC--QEGVPDGELKVLYNGTCQ-----------TCSDIVCKGGGDC-------VVDADSGRPICRCNHNCTEAQESDV---VCGSDGQTYPSQCEL-------DSTACREQTDLRLAYKGDCALCEGVQCAFGAHCAAG----ECVCPTDCTGAA--REPVCGSTMQTFPNECELQKAACRLPPPATLHVIFYGDCKDRLA 786 HSP 4 Score: 94.7449 bits (234), Expect = 8.159e-16 Identity = 84/280 (30.00%), Postives = 124/280 (44.29%), Query Frame = 0
Query: 5 LSEDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDA-NLCSSDPCPFNGVCARNYEGRFQCQC-QECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKC---ETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQ---NEDIFAEHYGACDPCNEVTCKFGSYC----SEGQCICR--QECPDSYEP--ICASNGKTYFNYCHLEKDNCARQHDANF 268
L+E + P C C CD++ R VCGSD+ Y + C L+ +C +Q +T +C A + C S C C + G C C EC R PVCGSD Y + C L ++SC ++ V G C C C C+ G +C C C + VC SD TY S C ++ +C + + ++ + G C C+++ CK G C G+ ICR C ++ E +C S+G+TY + C L+ C Q D
Sbjct: 435 LNEARAPVCRCGPPCDLL--VRPGSAVCGSDYKDYPSECALRRESCRTRQQLTIAYRGECASAQHPCDSVKCGSREHCTLDARGVAVCGCGAECEAVVR---PVCGSDGRTYDSRCHLDRASCLDNRDVRVAYTGPCGVENPCARASCPWGGACVA-RGGAAQCACPVC-DATLTPVCASDRNTYGSECKMRMHACQEGVPDGELKVLYNGTCQTCSDIVCKGGGDCVVDADSGRPICRCNHNCTEAQESDVVCGSDGQTYPSQCELDSTACREQTDLRL 707 HSP 5 Score: 81.6481 bits (200), Expect = 7.681e-12 Identity = 93/357 (26.05%), Postives = 148/357 (41.46%), Query Frame = 0
Query: 140 ETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYC--SEGQCICRQECPDS---YEPICASNGKTYFNYCHLEKDNCARQHDANFE---------QFRVVESRVCL---EKVPVC----PVDGCTCPHGVGGHLC---------------DRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFS----GSKCE-----ICPDGTLSTDSGC-ISVSSTKYERPI------------PCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRK 435
E C+ C EC+ +G C C VC + G TY + C+L++ +C + ++ +H G C DPC VTC G+ C + GQ CR CP S +P+C ++G+ Y + CHL+K C Q + + + + VC + PVC P D P G +C + C+T ++ EC + +S C ++ RG + G++CE +C + DS C + +S R + PC + C +G C GG +C C + C L VC +D NTY SEC++R ++C++
Sbjct: 279 EACEKTFCRWGAECVSLGDGRAHCACPTTCPTSPAPVCSTAGRTYRNHCYLRKEACERRLNLRVKHEGECDAGDPCTGVTCPAGARCVAAYGQAECR--CPRSCQRRKPVCGTDGREYSSVCHLDKHACDNQFNITIKYHGKCDPCSEHECADGGVCQLNEARAPVCRCGPPCDLLVRP---GSAVCGSDYKDYPSECALRRESCRTRQQLTIAYR-GECASAQHPCDSVKCGSREHCTLDARGVAVCGCGAECEAVVRPVCGSDGRTYDSRCHLDRASCLDNRDVRVAYTGPCGVENPCARASCPWGGACVARGGAAQCACPV-CDATL---TPVCASDRNTYGSECKMRMHACQE 625 HSP 6 Score: 73.9442 bits (180), Expect = 1.867e-9 Identity = 116/549 (21.13%), Postives = 203/549 (36.98%), Query Frame = 0
Query: 534 TCKSLSHKDTR-----PIHVSGL--------CDPNPC--HGGGTCE-EHDGVFSCYC----PPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNR-LEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRVT-GSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCE----------------DSDPESSWE---RYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIAS-------LETNLPISDGNWHRVNILRQDSSLIVHLDGEKS--------MFPEKLEYLDFVSHG-QLTFGPLTMRSLCLRHYVLKTWKNNRH 1025
TCKS+ T PI G C+ NPC G C+ DG C C G G C +D++ + + + L P + + + I++ F T +G++LY G + DF+++ +++ +++FRY+LGSG + S + G+WH R + G L++D G S L L + ++IG++ + + K G F G ++ + ++ ++L + ++ E+ R+PC+ G +TG C PCKN G C NE + C+C G+ GR CE D + S+ R S S+ R ++ F +T ++ + +RP + L L G+L + +T E +A+ +E+ + DG WH + R+ ++ +D + + + P K ++ L G LR V N RH
Sbjct: 1388 TCKSIDGSATEYTCSCPIGFQGANCDERLDPCESNPCGYDEGLLCDIGTDGGHVCRCLFGGEIGSNGNTCNNDVNVVQETWSPQFNGTSYIELPPLEGLGKAFRIEIWFLTNRFSGMLLYT-GQSNKAKGDFIAINLVNG-----------------------YLQFRYNLGSGIANITSPVPITKGRWHRARVSRSGRHGSLQLDHHPTQRGHSFPPLTHLELTLPLFIGSLPSYIRPH--KMSGVTSSFIGSVQQVFVNGKPLSLYGAD----TAKCTIVQDED----RLPCATTGV-------------TRYTGPPCGEDLT-----PCKNNGSCIPLLNE--YKCTCRDGYQGRNCEIQINVEMLNERTPVNFDGNNYFSYRSRGSRRNSGSRGIRYEIKF--RTYNDSGLLMWRRKIGIRPRDFIGLGLS---DGKLQLIYTDTDIKEMNLATHEDWFQNIESKARVDDGRWHTATVRRRKRLAMLQVDDKPAVRGYSQSLLVPSKANPKLWIGGSPSLPLGLPAALYTGLRGCVASVKANGRH 1877 HSP 7 Score: 68.9366 bits (167), Expect = 7.038e-8 Identity = 70/245 (28.57%), Postives = 101/245 (41.22%), Query Frame = 0
Query: 210 DPCNEVTCKFGSYC-----SEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSK-CEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLR-CICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPC 447
+ C + C++G+ C C C CP S P+C++ G+TY N+C+L K+ C R+ + RV C P G TCP G RC A +G EC C + C + VC G G + +C + D+ +++ + + PC E EC G C R C C C + AVCG+D Y SEC LR+ SCR + + I C
Sbjct: 279 EACEKTFCRWGAECVSLGDGRAHCACPTTCPTSPAPVCSTAGRTYRNHCYLRKEACERRLN-----LRVKHEGECDAGD---PCTGVTCPAGA------RCVAA------YGQAECRC------PRSCQRRKPVC----GTDGREYSSVCHLDKHACDNQ-FNITIKYHGKCDPCSEHECADGGVCQLNEARAPVCRCGPPCDLLVRPGSAVCGSDYKDYPSECALRRESCRTRQQLTIAYRGEC 492
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Match: gi|1233198130|ref|XP_022198600.1| (agrin-like, partial [Nilaparvata lugens]) HSP 1 Score: 375.941 bits (964), Expect = 2.839e-106 Identity = 317/1093 (29.00%), Postives = 473/1093 (43.28%), Query Frame = 0
Query: 27 GNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQC-QECYEEHREYEPVCGSDNVQYPNECELRKSSC---QQKVEITVKKYGKC--------------------------------------ETCQDKKCGPHEECIEDTNGNPKCLCQ-GCSNKP---SEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACD-----PCNEVTCKF----GSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKD--NCARQHDANFEQFRVVESRV--CLE-KVPVCPVDGCTCPH-------------------------GVGGHLCDRCKTAFWAFTSF-----GCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEIC--PDGTLSTDSGCISVSSTKYERPIP--CGESECNFGATCSNTGGRLRCICEIDCR--NPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRSTFDMELKMTLISLMTDAPDQIYGQSKSPRQVL-SDQK-----------------------------PSTGQ-----FGETCLTDIDCIVDNSHCSLNQCICDENHDYHADSRTCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVD-GDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSC-SVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVG-----FFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAA--DLFSIPVRLRPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLD 979
G P+CG+D VTY+N CEL+ AC ++ +I C+ LC C C C C EC + E EPVC S+ Y +EC +++ +C Q ITV YG C E C+D +C C + P+C C CS++P S VCGSD Y S C ++ +C + E++ C PCN F G G RQ+CP +YCH C R+ DA +S C + K P D CP GVGG CDRC+ +W GC CGC+ FGS DC+ TG C CK G G KC +C P LS SGC+ T P+P C ++ C FGA C + G+ +C+C I C + + VCG DG TY SECQL+ SCR Q DIA+ C+ + G T + K T ++ T+ D SKS R +L DQ+ P+T Q G+ C D DC + NS C CIC E D + C + P +C PC GG+CE G F C C G+ G LC+ L + + +F GNS + L A ++L ++++F+T+A NG++ YN+ + + G DF+SL I+D F+EFRY+LG+GPV+++S ++ + H + AKRY +DGLL+VD + V G S+G+LR L++ + ++G V + V + +GT +G GC++ +++ R ++ L ++ MV++ + V +C ENPC M C N G+C ++ ++C C +F G C+++ C +PC +C+ +E FTC CP G F + + PE S Y + K + +F L+ A + +L G + L + ++ R L S +A++ +N + WHRV++ R D + L+
Sbjct: 421 GGGPICGTDGVTYKNDCELRRVACQQKLSIQAAYKGDCD---LCQGVTCKLGARCEAGV-----CVCPTECPPDGSE-EPVCASNFQTYSSECHMQRQACLPPGQDPPITVLFYGDCNDRFGPAQITTPLLPGSLDGLPTRPTEVARPGGNQLEQEACRDIRCDYDATCELGPDNFPRCSCLFDCSHEPAAASGPVCGSDLRMYASKCAMKMEACQRQEELRLRPLDLCQGMEVKPCNGEPPIFDHKTGQELDCGSGPQRQDCPSD-------------SYCHQTPHFARCCRKADAGIFLKSCEDSWYGCCPDSKTPAQGPDNAGCPSMCGCNKIGSYSDTCDPESKQCECKPGVGGIKCDRCEPGYWGLPKISTGHQGCLPCGCSLFGSVRDDCEQMTGRCVCKPGVQGQKCTVCNNPKHVLSP-SGCVPADMTT---PVPTSCTDTTCYFGAVCEESNGKAKCVCSITCSEDSSSKVEQVVCGNDGQTYGSECQLKLFSCRYQKDIAVQALGSCKENMMVG--TEWPQHKWTSVNRFTEPEDVSSPMSKSTRHLLIPDQRYYYSNQVDEHVVSPMRSKELYHLVGPRPTPATIQVFTALLGDLCSFDSDCSIYNSICVTGACICKEGFMESTDRQECLG-KMNEIVPTAELDICSTEPCKNGGSCEPTLGSFQCICTAGYTGELCEDLLIPKNYEVPAFNGNSYVK-LKKLKAYHKLSLEIEFKTYANNGILFYNQQHAD-GTGDFVSLAIVDG-----------------------FIEFRYNLGNGPVIIKSEQRIEMRKMHRVTAKRYQRDGLLQVDSAEPVAGQSQGTLRALDLLQDAFVGYVPSD-TTKVFENLGTSMGLIGCIRRMKVGRHVVELHEGRDAMVLETEGVKECGENPCANMVCQNGGTCIPTGDERYKCSCTADFIGESCEQRLTPCVSSPCMLGSKCE-PLSEGGFTCRCPPGEPCQHFGMDIVDFPVPEFSGVSYLE-LPKLENVGRAFSLEVWFKAFAPDGVIVYNGQLNNGRGDFIVLNLVDG---FLQFRFDLGSG--VANITSNEAVELDQWHRVHVSRTDKEGTLQLN 1451 HSP 2 Score: 163.31 bits (412), Expect = 6.239e-37 Identity = 94/233 (40.34%), Postives = 128/233 (54.94%), Query Frame = 0
Query: 30 PVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECP--DSYEPICASNGKTYFNYCHLEKDNC 260
PVCGSD T+ + C L+ AAC ++ I C + CSS C +C G C+C C E+ PVCGSD + Y NEC+LR +CQ +ITV G C C++KKC C D+NG +C+C + +CG+DG+TY + C L+R +C Q I A + G CD C VTCK G+ C G C+C ECP S EP+CASN +TY + CH+++ C
Sbjct: 283 PVCGSDGKTHDSECHLRRAACKEKTVIKIAHTGACGASGPCSSHTCHPGAICVER-GGLPVCECPRC---SAEFNPVCGSDGIPYGNECKLRLEACQHMRDITVLYKGNCNGCENKKCEFFATCEIDSNGEAQCICPSNCPEGGGPICGTDGVTYKNDCELRRVACQQKLSIQAAYKGDCDLCQGVTCKLGARCEAGVCVCPTECPPDGSEEPVCASNFQTYSSECHMQRQAC 511 HSP 3 Score: 141.354 bits (355), Expect = 3.477e-30 Identity = 127/457 (27.79%), Postives = 192/457 (42.01%), Query Frame = 0
Query: 4 LLSEDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYE-GRFQCQC-QEC--YEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC----DPCNEVTCKFGSYC-----SEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVC-----PVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCN--EFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCS-NTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDI 439
L D C C C + PVCG+D TY N C L++ +C + NI +C+ + C CP C ++ G C C Q C Y +H P+C SDN Y + C +RK++C + +I++K +G C+ C++ +C E C D + P C C VCGSDG TY + C+L + +C + + G C +PC+ V C G C QC C EC P+C S+GKT+ + CHL + C + ++ + C P P C G+ C RC F CG + +G+E K C+ D T+ C C +C F ATC ++ G +CIC +C +CGTDG TY+++C+LR+ +C+++ I
Sbjct: 39 LPGSDGKALCRCPETC-----PQTAAPVCGTDGRTYSNLCALRQHSCLHRINIRVQHPGECDSVDPCLKKECPPGARCVVDHSSGEPTCVCPQRCPQYGDHAASRPLCASDNRDYRDLCHMRKAACTEGRDISLKLHGACDPCENIECVEPEVCQLDEDRKPVCRCGDTCPLEFTPVCGSDGKTYINECNLHQEACRTRRKLRIIYRGKCSSGVNPCSSVECSHGQECVITKYGIAQCECPAECEPVVRPVCGSDGKTHDSECHLRRAACKEK-----TVIKIAHTGACGASGPCSSHTCHPGAICVERGGLPVCECPRCSAEFNPV-------CGSDGIPYGNECK--------------LRLEACQHMRDITVLYKGNCNG-----------CENKKCEFFATCEIDSNGEAQCICPSNCPE---GGGPICGTDGVTYKNDCELRRVACQQKLSI 450 HSP 4 Score: 103.219 bits (256), Expect = 1.986e-18 Identity = 112/474 (23.63%), Postives = 184/474 (38.82%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQH-DLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEV-DGDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPC--VRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCP---------VGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSK---AADLFSIPV-------RLRPGNVGHLALKVSQSGQLVM----------------------ELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLD 979
C +PC G CE +G F+C CPPG CQH + + F G S + + + +++ F+ FA +G+I+YN G L G DF+ L ++D F++FR+DLGSG + SN V QWH + R K+G L++ +G V GSS L LN+ ++IG V + V + G G G ++ R L+ Q + V + PC PC N G C + C+C P F+G CQ + N+ + I R S P + +S E WE + K + ++ + L + + D++ + + N L++K S L++ EL L ++ + +L + ++DG WHR+ + R+ + +D
Sbjct: 1310 CVSSPCMLGSKCEPLSEGGFTCRCPPGEP---CQHFGMDIVDFPVPEFSGVSYLELPKLENVGRAFSLEVWFKAFAPDGVIVYN-GQLNNGRGDFIVLNLVDG-----------------------FLQFRFDLGSGVANITSNEAVELDQWHRVHVSRTDKEGTLQLNNGSVVRGSSGPPLNELNLELPLYIGGVPDV--NQVSRDAGIVTGMLGAVQ-----RVLLNGQPWGRLDTTGGQQVARYQGAPCPPANNPCLNSGLCLPILNSYICKCLPNFSGDNCQTADD-------VNEDEVAI-RFSGSIFLQYPQKLPRMHKSINSSSENFTNSVVEDYWESEEHAEYKDEEEETAVELDSEMDPEGGEGDMYDEDDFEFFDERKGKRNNRYELSIKTSDLNGLLLWTNRGKTLHADYFAVAVVDGFPELSYNLGNQHKLLTLRAPVLVNDGEWHRITVHRRKRQAQLQVD 1741 HSP 5 Score: 96.2857 bits (238), Expect = 3.040e-16 Identity = 97/345 (28.12%), Postives = 140/345 (40.58%), Query Frame = 0
Query: 140 ETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYC------SEGQCICRQECPD-----SYEPICASNGKTYFNYCHLEKDNCARQHDANF---------EQFRVVESRVCL---EKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTG-GRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRST 457
E C+ C C+ ++G C C + + VCG+DG TY +LC L++ SC +I +H G C DPC + C G+ C E C+C Q CP + P+CAS+ + Y + CH+ K C D + E VE VC ++ PVC G TCP FT CG ++ + +C+ C +R K I G S+ PC EC+ G C T G +C C +C + VCG+DG T+ SEC LR+ +C+++T I I + C C T
Sbjct: 25 EPCEKTYCSHGATCLPGSDGKALCRCPETCPQTAAPVCGTDGRTYSNLCALRQHSCLHRINIRVQHPGECDSVDPCLKKECPPGARCVVDHSSGEPTCVCPQRCPQYGDHAASRPLCASDNRDYRDLCHMRKAACTEGRDISLKLHGACDPCENIECVEPEVCQLDEDRKPVCRC-GDTCP---------------LEFTPV----CG-SDGKTYINECNLHQEACRTRR-----KLRIIYRGKCSSGVN-------------PCSSVECSHGQECVITKYGIAQCECPAECEPVV---RPVCGSDGKTHDSECHLRRAACKEKTVIKIAHTGACGASGPCSSHT 327
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Match: gi|1285008444|ref|XP_023016157.1| (agrin-like [Leptinotarsa decemlineata]) HSP 1 Score: 371.703 bits (953), Expect = 6.399e-106 Identity = 317/1122 (28.25%), Postives = 471/1122 (41.98%), Query Frame = 0
Query: 13 CACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVE---ITVKKYGKC----------------------------------------------ETCQDKKCGPHEECIEDTNGNPKCLCQ-GCSN---KPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCK---------FGSYCSEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCAR--QHDANFEQFRVVESRVCLEKVPVCPVDG----------------------------------CTCPHGVGGHLCDRCKTAFWAFTSF-----GCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPD-GTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRST-----------------FDMELKMTLISLMTDAPDQIYGQSKSPRQVLSDQKPSTGQF------------------GETCLTDIDCIVDNSHCSLNQCICDENHDYHADSRTC--KSLSHKDTRPIHVSGLCDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVD-GDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRLRPGNVGHLALKVSQ----SGQLV--------MELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLD 979
C C C D N VCG+D TY N CEL+ A+C +Q I C+ LC C + C +C C E + EPVC S+ + YPNECEL+K+ C Q ++V YG C E C+D C C + P+C CQ C+N S+ VC SD Y SLC ++ +C + E++ C CK G G RQ+CP +YCH + +R D NF +E + C + C DG C C GVGG CDRC++ FW GC CGC+ FGS DC+ TG C C+ G G KC +C + + +GC+S + P C E C FGATC+ GG C C C + + VCG+DG TY S C+LRQ +CR Q DI + F C+ RST + L + L+ P Y +S + R + +G+F G+ C + DC + SHC C C +H +D + C KS S + R C +PCH TC + F+C C F G LC+ ++ ++ I +F G S + L P A ++L I+++F+++ +NG++L+N+ L+ G DF+SL +++ FVEF+Y+LG+G V++RS ++ G +H +V KRYH+DG+L++D + V G + GSL+ L++ E ++G V T Y V + +GT GF GC++ L++ R I L Q+ V++ K + +C ++ C + PC N G C + + CECP +G C++ + C NPC+ +C+ + +SF C CP+G G C E ++ + T +++ SF L FSI V P L L Q G + ++ R L S IA+L + P+S WH V I R + +D
Sbjct: 31 CVCPQSCS--DPKLNNGTVCGTDGKTYANECELRMASCKTKQYIILAYKGNCD---LCQEVECKYGSRCEAG-----ECVCPTNCEGSGD-EPVCASNMMTYPNECELQKAMCLQATNSPPLSVVFYGDCRERFPVAGSLSTTLPTITVQTNSVTGTDLETAEPDDSVLEAGNTEKEACKDIHCDFEATCELGPDNFPRCTCQFDCANALSSASKPVCASDLRIYPSLCAMKMEACQRQEELRLRPLDLCQGLEVKPCKGEKPLTDVITGRELDCGNGPFRQDCPSG-------------SYCH-QTTRFSRCCHKDKNF-----IEKKSCEDSWYGCCPDGKNVAQGPNFAGCPSLCGCHKLGSYSEMCDPDTQQCKCRPGVGGAKCDRCESGFWGLPKISSGYRGCIPCGCSTFGSVRDDCEQMTGRCVCRLGVQGQKCTVCTNHNKVLGPNGCVSAADYTTSPPTTCKELTCYFGATCTERGGLAICECHTKCPEDIE-TQVVCGSDGQTYSSPCELRQLACRLQRDIVVQAFGTCKEETI--RSTDWPSRRYTPLQFTQPDESNSPLSKSTRHLLVPDPRYYYERSGNMRPISYPADFDSGEFASAYRPTPATVRVVTALLGDLCSDNSDCSILYSHCVKGACTCIADHAESSDRQECIAKSESTDEYRA------CTSSPCHHASTCVDLPTATFTCVCNSNFTGLLCETEMLRTQYDIPAFHGTSYVR-LKPLKAYHKLSIEVEFKSYTENGVLLFNQQKLD-GLGDFVSLALVNG-----------------------FVEFKYNLGNGAVLIRSVDKIQLGNFHRVVLKRYHRDGILKLDEAEDVAGQAMGSLKALDLLEDTYVGFVPTNYS-RVYENIGTKKGFQGCIRKLKIGRQEIQLNMNQDDWVLKTKGIHNCSQHACSQFPCLNNGKCIPVDDLYRCECPLHHSGEICEKLDDPCSLNPCRYGSKCR--PHLDSFICECPIGRSGHHC-----EIEYKDVQIVTPEFNGS--SFIQFPRLEGIKKTFSIEVSFMPKASNGLILYNGQMKNGRGDFISLNLARGYLQFRFNLGSG--IANLTSGEPLSLNKWHWVRISRNGREGKMQVD 1076 HSP 2 Score: 115.161 bits (287), Expect = 3.851e-22 Identity = 119/478 (24.90%), Postives = 186/478 (38.91%), Query Frame = 0
Query: 545 PIHVSG-----LCDP---NPCHGGGTCEEHDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDGDRVT-GSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRK-NVIDCEEN-------PCV--RMPCSNEGSCSVKNQGFECECPPEFTGRRCQR--------KRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQ--TNLSKAADLFSIPVRLRPGNVGHLALKVSQSGQLVM------------ELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGE 981
P+H SG L DP NPC G C H F C CP G +G C+ + I F G+S + I++ F A NGLILYN G ++ G DF+SL + +++FR++LGSG L S + +WH + R ++G ++VD + G S L LN+ ++IG+V + + L G GF G ++ + L+ + + V+ N C N PC + PCSN G C F C+CP F G+ C+ K N +NKG R++N + + E++ +S E ++ YD F L+ L K + +R L L S++ V EL L ++ + + DG WH +++ R+ + +DGE
Sbjct: 919 PLHHSGEICEKLDDPCSLNPCRYGSKCRPHLDSFICECPIGRSGHHCEIEYKDVQIVTPEFNGSSFIQFPRLEGIKKTFSIEVSFMPKASNGLILYN-GQMKNGRGDFISLNLARG-----------------------YLQFRFNLGSGIANLTSGEPLSLNKWHWVRISRNGREGKMQVDNSSIVRGFSGTPLTELNLETSLYIGSVANWKQIHRLS--GASKGFKGAIQGILLNGNPLPISRLLPDCVIHAAYNNSGCSSNIGFYDGLPCSLSKNPCSNNGLCVPDLGDFSCKCPSNFKGKYCETVKEESLALKLNGITFLQYRNKGY----RSDN-------ITYDFEYGEETRNATSGEEINENNYLYDENNFDFDLEDMETLRKPERGNRYELLIRTFMPDGLLLWRSRNRNHVQDYLSIAIVDGYPELSFNLGHRSSFWAVRSKRRVDDGRWHTIHVRRRKRVGFISVDGE 1359 HSP 3 Score: 105.916 bits (263), Expect = 2.546e-19 Identity = 93/314 (29.62%), Postives = 130/314 (41.40%), Query Frame = 0
Query: 142 CQDKKCGPHEECIEDTNGNPKCLC-QGCSNKP--SEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQCICRQECPDSY-EPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRL-RCICEIDCRNPL-YYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQG 449
C+ +KC + C D +C+C Q CS+ + VCG+DG TY + C L+ ASC + I + G CD C EV CK+GS C G+C+C C S EP+CASN TY N C L+K C + N VV C E+ PV T P QT +G+ E T D + +T+ E C + C+F ATC RC C+ DC N L + VC +D Y S C ++ +C++Q ++ + CQG
Sbjct: 10 CESRKCDFYSSCESDGATEGRCVCPQSCSDPKLNNGTVCGTDGKTYANECELRMASCKTKQYIILAYKGNCDLCQEVECKYGSRCEAGECVCPTNCEGSGDEPVCASNMMTYPNECELQKAMCLQA--TNSPPLSVVFYGDCRERFPVAGSLSTTLP------------------------------------TITVQT------NSVTGTDLE-----TAEPDDSVLEAGNTEKE---ACKDIHCDFEATCELGPDNFPRCTCQFDCANALSSASKPVCASDLRIYPSLCAMKMEACQRQEELRLRPLDLCQG 271
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Match: gi|1101350309|ref|XP_018901099.1| (PREDICTED: agrin-like isoform X1 [Bemisia tabaci]) HSP 1 Score: 373.629 bits (958), Expect = 4.027e-105 Identity = 317/1096 (28.92%), Postives = 468/1096 (42.70%), Query Frame = 0
Query: 31 VCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREY---------EPVCGSDNVQYPNECELRKSSC--QQKVEITVKKYGKC-----------------------------------------ETCQDKKCGPHEECIEDTNGNPKCLCQ-GCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACD-----PCNE----VTCKFGSYCSEGQCICRQECP-DSY---EPI----CASNGKTYFNYCHLEKDNC--ARQHDANFEQFRVVESRVCLEKVPVCP------VDGCTCPHGVGGHLCDRCKTAFWAFTSF-----GCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRSTFDMELKM---TLISLMTDAPDQIYGQSKSPRQVLSDQK----------------------PSTGQF-------------------GETCLTDIDCIVDNSHCSLNQCICDENHDYHADSRTCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVD-GDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCS-VKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPV----RLRPGNVGHLALKVSQSGQLV--------MELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGEKSM 984
VCGSD TYR+ CEL+ +C +Q +T S C + GV A E + C E+ EPVC SD V YP+ECE+ + +C Q++ TV YG C E C+D C C +G P+C C CS + ++ VCGSD Y S C ++ +C Q +++ C PCN V G G RQ+CP DSY P C + K + C C + A+ S K+ + C C GVGG CDRC+ FW GC CGC+ FGS +DC+ +G C CK G G KC C D T ++ + P C E C F + C G +C+C + C + VCG DG TY SECQL+ +CR Q DI++ + C+ + G D +K+ T TD+ SKS R +L + + P+ F GE C +D DC V +SHC C+C EN+ D + C + T + CD PC GG C G+F C C G+ GTLC + IA+F G S A L A ++L I+++ +T+A NG++LY++ + E G DF+SL I++ FVEFRY+LG+GPVV+ S +V ++H +VAKRY +DGLL+VD + V G S G+LR L++ + ++G V T V + +GT G GC++ +++ R + L ++P +++ + DC ENPC PC N +C + + + C CP FTG +C+ + + C NPC C ++ ++N FTC C G G +C+ DP+ E + T ++ + L L A FS+ V R G + + + G + ++ R L S IA++ T PIS WH+V + R D ++ +D ++
Sbjct: 791 VCGSDGATYRSECELRLESCKSRQLVTVASQGDCGASK---------TGVAASGAEA------EGCDEKALAVCGGNGSAANEPVCASDLVTYPSECEMVRRACTAQKRPLPTVLYYGDCKEKFALGPSSLTTPTTFEKLSLQPTDSSSLENSVFSNSAEKEACKDIHCDFDATCELGPDGYPRCSCLFDCSREATKLVCGSDLKVYQSSCAMKMEACQQQKELRLRPLDLCQGMEVKPCNGEQPLVDPTSGHELDCGSGPRRQDCPSDSYCHQTPYFARCCRKDAKIFLKNCADSWYGCCPDNKTPASGPDNAGCPSLCGCNKLGSYSDNCKNGTEQCHCRPGVGGIKCDRCEPGFWGLPKISDGHQGCMPCGCSVFGSVREDCEQMSGRCVCKPGVQGPKCNQCADPTKVLGPNGCALPDALGQIPATCAEMHCYFESICEERNGLPKCLCNMTCPEESNSNQVVCGNDGQTYASECQLKLYACRYQKDISVQYLSACKDEVLTGT---DWPVKLGGWTSAQHFTDSEALSSPLSKSTRHLLPEHRYYYTNKDSIPEDDISGFVSAGGPTKDLFSEEGKDSTPAIVEVMHSSLGEMCSSDEDCRVSHSHCLSGLCVCLENYVQTQDRQACYLDAIDPTAEVLA---CDSLPCLNGGRCRNLPHGLFECICEEGYQGTLCAEIAERKEYKIAAFNGQS-YAQLHSIKAYHKLSIEMELKTYANNGILLYDQQH-EDGTGDFISLAIVN-----------------------GFVEFRYNLGNGPVVIVSPHKVTLRKFHRIVAKRYQRDGLLQVDSAEPVAGQSLGTLRALDLSQDAYVGFVPTNVS-KVHENIGTSHGLNGCIRHMKIGRHTVYLHGGEDPFIVKVNQIEDCGENPCSSAPCHNGATCKPLDAESYACTCPDSFTGVQCESRMDPCVSNPCSFGSTC-VSLHQNGFTCKCLPGRKGNMCQHFDPD--LEDF--FTPEFAEENAYIEL-PKLENAGRSFSLEVWFMAREPDGVILYNGQMTNGKGDFISLNLVNGYLQFRFDLGSG--IANITTPEPISLNEWHKVEMSRMDRRGVLRVDNTTTV 1831 HSP 2 Score: 150.599 bits (379), Expect = 5.437e-33 Identity = 95/255 (37.25%), Postives = 125/255 (49.02%), Query Frame = 0
Query: 27 GNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQ-CQCQE---CYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC----DPCNEVTCKFGSYC-----SEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANF 268
G VCGSD TYR+ C L+ AAC Q++I QCE A+ C CPF C R+ EG C C E Y +H P+CGSD Y + CEL K++C K+ IT+K GKC+ C C E C D + NP C C VCGSDG TY + C+L+ +C +++ + G C +PC V C FG C QC C C P+C +NG+TY CHL++ C + D
Sbjct: 423 GGPSVCGSDGNTYRSLCHLRLAACRTQRSIRVRYSGQCEVADPCQDFSCPFGAKCTRSKEGNSPVCLCPEHCPSYGDHVGSRPICGSDGNDYTHHCELTKAACVHKLNITIKYSGKCDPCAGVVCPEPELCQLDADRNPVCKCGEACPLEFAPVCGSDGKTYGNDCNLRLEACRSKKNLRIIYRGKCSSGVNPCASVRCTFGEECVINKFGIAQCECISNCDPILRPVCGTNGRTYDTACHLQQSTCQAKTDVKL 677 HSP 3 Score: 133.65 bits (335), Expect = 1.137e-27 Identity = 95/285 (33.33%), Postives = 140/285 (49.12%), Query Frame = 0
Query: 7 EDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCET----CQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYCSE--GQCICRQECP---DSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVC 279
E P C C C + G+ P+CGSD Y + CEL +AAC + NIT +C+ C+ CP +C + + C+C E E+ PVCGSD Y N+C LR +C+ K + + GKC + C +C EEC+ + G +C C + VCG++G TY + CHLQ+++C D+ + G C PC+ C+FG+ C E G +C ECP +P+C S+G TY N C L ++C QH Q R++ +C
Sbjct: 482 EGNSPVCLCPEHCPSYGDHVGSRPICGSDGNDYTHHCELTKAACVHKLNITIKYSGKCDP---CAGVVCPEPELCQLDADRNPVCKCGEACP--LEFAPVCGSDGKTYGNDCNLRLEACRSKKNLRIIYRGKCSSGVNPCASVRCTFGEECVINKFGIAQCECISNCDPILRPVCGTNGRTYDTACHLQQSTCQAKTDVKLAYAGICGGNGPCSARKCEFGATCVERGGSPVC--ECPVCSTELDPVCGSDGITYGNECKLRLESC--QH---HRQIRILHHGLC 754 HSP 4 Score: 130.183 bits (326), Expect = 1.152e-26 Identity = 121/399 (30.33%), Postives = 165/399 (41.35%), Query Frame = 0
Query: 70 CSSDPCPFNGVCARNYEGRFQCQC-QECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCET---CQDKKCGPHEECIEDTNGN-PKCLC-QGC----SNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQ-----CICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERP------IPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPC 447
C C +C+ + G +C C EC E VCGSD Y + C LR ++C+ + I V+ G+CE CQD C +C GN P CLC + C + S +CGSDG Y C L +A+C +I ++ G CDPC V C C C C + CP + P+C S+GKTY N C+L + C + + R++ C V C CT FG EC N+FG C+ + P R G+ S C + + K PC +C FGATC GG C C + C L + VCG+DG TY +EC+LR SC+ I I + C
Sbjct: 392 CEKTTCAHGALCSADERGDARCHCPAECPEGGG--PSVCGSDGNTYRSLCHLRLAACRTQRSIRVRYSGQCEVADPCQDFSCPFGAKCTRSKEGNSPVCLCPEHCPSYGDHVGSRPICGSDGNDYTHHCELTKAACVHKLNITIKYSGKCDPCAGVVCPEPELCQLDADRNPVCKCGEACPLEFAPVCGSDGKTYGNDCNLRLEACRSK-----KNLRIIYRGKCSSGVNPCASVRCT----------------------FG-EECVINKFG--IAQCECISNCDPILRPVCGTNGRTYDTACHLQQSTCQAKTDVKLAYAGICGGNGPCSARKCEFGATCVERGGSPVCECPV-CSTEL---DPVCGSDGITYGNECKLRLESCQHHRQIRILHHGLC 754 HSP 5 Score: 117.472 bits (293), Expect = 9.607e-23 Identity = 82/255 (32.16%), Postives = 116/255 (45.49%), Query Frame = 0
Query: 13 CACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSY---CSE-GQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQ 263
C C + CD I PVCG++ TY C L+++ C + ++ C CS+ C F C G C+C C E +PVCGSD + Y NEC+LR SCQ +I + +G C +C++KKC +E C D+ G C+C VCGSDG TY S C L+ SC + + G C G+ C E +C + EP+CAS+ TY + C + + C Q
Sbjct: 637 CECISNCDPILR-----PVCGTNGRTYDTACHLQQSTCQAKTDVKLAYAGICGGNGPCSARKCEFGATCVER-GGSPVCECPVC---STELDPVCGSDGITYGNECKLRLESCQHHRQIRILHHGLCNSCENKKCETYEICQNDSYGEAHCVCPPSCPPEESPVCGSDGATYRSECELRLESCKSRQLVTVASQGDCGASKTGVAASGAEAEGCDEKALAVCGGNGSAANEPVCASDLVTYPSECEMVRRACTAQ 882 HSP 6 Score: 117.087 bits (292), Expect = 1.073e-22 Identity = 112/452 (24.78%), Postives = 185/452 (40.93%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQH-DLSKSSISIASFVG-NSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDG-DRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPC--VRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESS---WERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRL-------------RPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGE 981
C NPC G TC H F+C C PG G +CQH D F N+ + + +A +++ F +G+ILYN G + G+ DF+SL +++ +++FR+DLGSG + + + +WH++ R + G+L VD V G+S L LN+ +++G V + E V + G G G ++ + ++ I L + + V + + PC V PC N G C F C+C P F G CQ++ N G+ + + S + + V L E D E + ++ + + YD SF K + + + VR R + AL V +EL L E + S +N+ +SDG WH + + R+ ++ +D E
Sbjct: 1681 CVSNPCSFGSTCVSLHQNGFTCKCLPGRKGNMCQHFDPDLEDFFTPEFAEENAYIELPKLENAGRSFSLEVWFMAREPDGVILYN-GQMTNGKGDFISLNLVNG-----------------------YLQFRFDLGSGIANITTPEPISLNEWHKVEMSRMDRRGVLRVDNTTTVEGTSGAPLNELNLELHLYVGGVPSLSE--VSREAGITAGLNGAVQRVLMNGLPIRLFNRRSNQVAKYQG------PPCSVVDNPCLNGGLCLPLLSSFACKCHPPFIGATCQQQNPELEVAVSFN-GETFLQYPKKSPSLNRSVANSSDLTEVVDLERTLDLYDDFEEEEDMYDSDDTSFFNDERKGKRTNKYELSVRTTEKNGLLLWSNRGRNNQGNYFALAVVDG---AVELSFNLGKEQRLLSARSNVYVSDGVWHSITVQRRKKMALLRIDNE 2096
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Match: gi|1101350313|ref|XP_018901101.1| (PREDICTED: agrin-like isoform X3 [Bemisia tabaci]) HSP 1 Score: 372.859 bits (956), Expect = 6.302e-105 Identity = 308/1052 (29.28%), Postives = 454/1052 (43.16%), Query Frame = 0
Query: 31 VCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREY---------EPVCGSDNVQYPNECELRKSSC--QQKVEITVKKYGKC-----------------------------------------ETCQDKKCGPHEECIEDTNGNPKCLCQ-GCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACD-----PCNE----VTCKFGSYCSEGQCICRQECP-DSY-------EPICASNGKTYFNYCHLEKDNC--ARQHDANFEQFRVVESRVCLEKVPVCP------VDGCTCPHGVGGHLCDRCKTAFWAFTSF-----GCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERPIPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPCQGRITCGRSTFDMELKMTLISLMTDAPDQIYGQSKSPRQVLSDQKPSTGQFGETCLTDIDCIVDNSHCSLNQCICDENHDYHADSRTCKSLSHKDTRPIHVSGLCDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQHDLSKSSISIASFVGNSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVD-GDRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPCVRMPCSNEGSCS-VKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESSWERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPV----RLRPGNVGHLALKVSQSGQLV--------MELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGEKSM 984
VCGSD TYR+ CEL+ +C +Q +T S C + GV A E + C E+ EPVC SD V YP+ECE+ + +C Q++ TV YG C E C+D C C +G P+C C CS + ++ VCGSD Y S C ++ +C Q +++ C PCN V G G RQ+CP DSY C + K + C C + A+ S K+ + C C GVGG CDRC+ FW GC CGC+ FGS +DC+ +G C CK G G KC C D T ++ + P C E C F + C G +C+C + C + VCG DG TY SECQL+ +CR Q DI++ + C + F E G+ +P V + GE C +D DC V +SHC C+C EN+ D + C + T + CD PC GG C G+F C C G+ GTLC + IA+F G S A L A ++L I+++ +T+A NG++LY++ + E G DF+SL I++ FVEFRY+LG+GPVV+ S +V ++H +VAKRY +DGLL+VD + V G S G+LR L++ + ++G V T V + +GT G GC++ +++ R + L ++P +++ + DC ENPC PC N +C + + + C CP FTG +C+ + + C NPC C ++ ++N FTC C G G +C+ DP+ E + T ++ + L L A FS+ V R G + + + G + ++ R L S IA++ T PIS WH+V + R D ++ +D ++
Sbjct: 791 VCGSDGATYRSECELRLESCKSRQLVTVASQGDCGASK---------TGVAASGAEA------EGCDEKALAVCGGNGSAANEPVCASDLVTYPSECEMVRRACTAQKRPLPTVLYYGDCKEKFALGPSSLTTPTTFEKLSLQPTDSSSLENSVFSNSAEKEACKDIHCDFDATCELGPDGYPRCSCLFDCSREATKLVCGSDLKVYQSSCAMKMEACQQQKELRLRPLDLCQGMEVKPCNGEQPLVDPTSGHELDCGSGPRRQDCPSDSYCHQTPYFARCCRKDAKIFLKNCADSWYGCCPDNKTPASGPDNAGCPSLCGCNKLGSYSDNCKNGTEQCHCRPGVGGIKCDRCEPGFWGLPKISDGHQGCMPCGCSVFGSVREDCEQMSGRCVCKPGVQGPKCNQCADPTKVLGPNGCALPDALGQIPATCAEMHCYFESICEERNGLPKCLCNMTCPEESNSNQVVCGNDGQTYASECQLKLYACRYQKDISVQYLSAC-------KDLFSEE-----------------GKDSTPAIV----EVMHSSLGEMCSSDEDCRVSHSHCLSGLCVCLENYVQTQDRQACYLDAIDPTAEVLA---CDSLPCLNGGRCRNLPHGLFECICEEGYQGTLCAEIAERKEYKIAAFNGQS-YAQLHSIKAYHKLSIEMELKTYANNGILLYDQQH-EDGTGDFISLAIVNG-----------------------FVEFRYNLGNGPVVIVSPHKVTLRKFHRIVAKRYQRDGLLQVDSAEPVAGQSLGTLRALDLSQDAYVGFVPTNVS-KVHENIGTSHGLNGCIRHMKIGRHTVYLHGGEDPFIVKVNQIEDCGENPCSSAPCHNGATCKPLDAESYACTCPDSFTGVQCESRMDPCVSNPCSFGSTC-VSLHQNGFTCKCLPGRKGNMCQHFDPD--LEDF--FTPEFAEENAYIEL-PKLENAGRSFSLEVWFMAREPDGVILYNGQMTNGKGDFISLNLVNGYLQFRFDLGSG--IANITTPEPISLNEWHKVEMSRMDRRGVLRVDNTTTV 1762 HSP 2 Score: 150.599 bits (379), Expect = 5.889e-33 Identity = 95/255 (37.25%), Postives = 125/255 (49.02%), Query Frame = 0
Query: 27 GNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQ-CQCQE---CYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC----DPCNEVTCKFGSYC-----SEGQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANF 268
G VCGSD TYR+ C L+ AAC Q++I QCE A+ C CPF C R+ EG C C E Y +H P+CGSD Y + CEL K++C K+ IT+K GKC+ C C E C D + NP C C VCGSDG TY + C+L+ +C +++ + G C +PC V C FG C QC C C P+C +NG+TY CHL++ C + D
Sbjct: 423 GGPSVCGSDGNTYRSLCHLRLAACRTQRSIRVRYSGQCEVADPCQDFSCPFGAKCTRSKEGNSPVCLCPEHCPSYGDHVGSRPICGSDGNDYTHHCELTKAACVHKLNITIKYSGKCDPCAGVVCPEPELCQLDADRNPVCKCGEACPLEFAPVCGSDGKTYGNDCNLRLEACRSKKNLRIIYRGKCSSGVNPCASVRCTFGEECVINKFGIAQCECISNCDPILRPVCGTNGRTYDTACHLQQSTCQAKTDVKL 677 HSP 3 Score: 133.265 bits (334), Expect = 1.287e-27 Identity = 95/285 (33.33%), Postives = 140/285 (49.12%), Query Frame = 0
Query: 7 EDQVPFCACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCET----CQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGAC---DPCNEVTCKFGSYCSE--GQCICRQECP---DSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVC 279
E P C C C + G+ P+CGSD Y + CEL +AAC + NIT +C+ C+ CP +C + + C+C E E+ PVCGSD Y N+C LR +C+ K + + GKC + C +C EEC+ + G +C C + VCG++G TY + CHLQ+++C D+ + G C PC+ C+FG+ C E G +C ECP +P+C S+G TY N C L ++C QH Q R++ +C
Sbjct: 482 EGNSPVCLCPEHCPSYGDHVGSRPICGSDGNDYTHHCELTKAACVHKLNITIKYSGKCDP---CAGVVCPEPELCQLDADRNPVCKCGEACP--LEFAPVCGSDGKTYGNDCNLRLEACRSKKNLRIIYRGKCSSGVNPCASVRCTFGEECVINKFGIAQCECISNCDPILRPVCGTNGRTYDTACHLQQSTCQAKTDVKLAYAGICGGNGPCSARKCEFGATCVERGGSPVC--ECPVCSTELDPVCGSDGITYGNECKLRLESC--QH---HRQIRILHHGLC 754 HSP 4 Score: 130.183 bits (326), Expect = 1.272e-26 Identity = 121/399 (30.33%), Postives = 165/399 (41.35%), Query Frame = 0
Query: 70 CSSDPCPFNGVCARNYEGRFQCQC-QECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCET---CQDKKCGPHEECIEDTNGN-PKCLC-QGC----SNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSYCSEGQ-----CICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQHDANFEQFRVVESRVCLEKVPVCPVDGCTCPHGVGGHLCDRCKTAFWAFTSFGCRECGCNEFGSESKDCDAQTGVCPCKRGFSGSKCEICPDGTLSTDSGCISVSSTKYERP------IPCGESECNFGATCSNTGGRLRCICEIDCRNPLYYREAVCGTDGNTYRSECQLRQNSCRKQTDIAITNFAPC 447
C C +C+ + G +C C EC E VCGSD Y + C LR ++C+ + I V+ G+CE CQD C +C GN P CLC + C + S +CGSDG Y C L +A+C +I ++ G CDPC V C C C C + CP + P+C S+GKTY N C+L + C + + R++ C V C CT FG EC N+FG C+ + P R G+ S C + + K PC +C FGATC GG C C + C L + VCG+DG TY +EC+LR SC+ I I + C
Sbjct: 392 CEKTTCAHGALCSADERGDARCHCPAECPEGGG--PSVCGSDGNTYRSLCHLRLAACRTQRSIRVRYSGQCEVADPCQDFSCPFGAKCTRSKEGNSPVCLCPEHCPSYGDHVGSRPICGSDGNDYTHHCELTKAACVHKLNITIKYSGKCDPCAGVVCPEPELCQLDADRNPVCKCGEACPLEFAPVCGSDGKTYGNDCNLRLEACRSK-----KNLRIIYRGKCSSGVNPCASVRCT----------------------FG-EECVINKFG--IAQCECISNCDPILRPVCGTNGRTYDTACHLQQSTCQAKTDVKLAYAGICGGNGPCSARKCEFGATCVERGGSPVCECPV-CSTEL---DPVCGSDGITYGNECKLRLESCQHHRQIRILHHGLC 754 HSP 5 Score: 117.472 bits (293), Expect = 9.271e-23 Identity = 112/452 (24.78%), Postives = 185/452 (40.93%), Query Frame = 0
Query: 552 CDPNPCHGGGTCEE-HDGVFSCYCPPGFAGTLCQH-DLSKSSISIASFVG-NSLMAVLAPADAVNRLEIKLKFRTFAQNGLILYNEGNLETGETDFLSLTIIDRCECPMIASTTQRAHDCYHSPYFRFVEFRYDLGSGPVVLRSNAQVVPGQWHELVAKRYHKDGLLEVDG-DRVTGSSKGSLRTLNVGELIWIGNVDTQYEDNVLKRVGTGIGFTGCLKDLELSRTLIALQSEQEPMVMQRKNVIDCEENPC--VRMPCSNEGSCSVKNQGFECECPPEFTGRRCQRKRNHCHPNPCKNKGQCQIARNENSFTCSCPVGFFGRLCEDSDPESS---WERYRKSTSKYDRQKVSFGLQTNLSKAADLFSIPVRL-------------RPGNVGHLALKVSQSGQLVMELRETLESEPVIASLETNLPISDGNWHRVNILRQDSSLIVHLDGE 981
C NPC G TC H F+C C PG G +CQH D F N+ + + +A +++ F +G+ILYN G + G+ DF+SL +++ +++FR+DLGSG + + + +WH++ R + G+L VD V G+S L LN+ +++G V + E V + G G G ++ + ++ I L + + V + + PC V PC N G C F C+C P F G CQ++ N G+ + + S + + V L E D E + ++ + + YD SF K + + + VR R + AL V +EL L E + S +N+ +SDG WH + + R+ ++ +D E
Sbjct: 1612 CVSNPCSFGSTCVSLHQNGFTCKCLPGRKGNMCQHFDPDLEDFFTPEFAEENAYIELPKLENAGRSFSLEVWFMAREPDGVILYN-GQMTNGKGDFISLNLVNG-----------------------YLQFRFDLGSGIANITTPEPISLNEWHKVEMSRMDRRGVLRVDNTTTVEGTSGAPLNELNLELHLYVGGVPSLSE--VSREAGITAGLNGAVQRVLMNGLPIRLFNRRSNQVAKYQG------PPCSVVDNPCLNGGLCLPLLSSFACKCHPPFIGATCQQQNPELEVAVSFN-GETFLQYPKKSPSLNRSVANSSDLTEVVDLERTLDLYDDFEEEEDMYDSDDTSFFNDERKGKRTNKYELSVRTTEKNGLLLWSNRGRNNQGNYFALAVVDG---AVELSFNLGKEQRLLSARSNVYVSDGVWHSITVQRRKKMALLRIDNE 2027 HSP 6 Score: 117.087 bits (292), Expect = 1.196e-22 Identity = 82/255 (32.16%), Postives = 116/255 (45.49%), Query Frame = 0
Query: 13 CACENLCDVIDEARGNVPVCGSDHVTYRNGCELKEAACGKQQNITQISDQQCEDANLCSSDPCPFNGVCARNYEGRFQCQCQECYEEHREYEPVCGSDNVQYPNECELRKSSCQQKVEITVKKYGKCETCQDKKCGPHEECIEDTNGNPKCLCQGCSNKPSEEVCGSDGITYFSLCHLQRASCTQNEDIFAEHYGACDPCNEVTCKFGSY---CSE-GQCICRQECPDSYEPICASNGKTYFNYCHLEKDNCARQ 263
C C + CD I PVCG++ TY C L+++ C + ++ C CS+ C F C G C+C C E +PVCGSD + Y NEC+LR SCQ +I + +G C +C++KKC +E C D+ G C+C VCGSDG TY S C L+ SC + + G C G+ C E +C + EP+CAS+ TY + C + + C Q
Sbjct: 637 CECISNCDPILR-----PVCGTNGRTYDTACHLQQSTCQAKTDVKLAYAGICGGNGPCSARKCEFGATCVER-GGSPVCECPVC---STELDPVCGSDGITYGNECKLRLESCQHHRQIRILHHGLCNSCENKKCETYEICQNDSYGEAHCVCPPSCPPEESPVCGSDGATYRSECELRLESCKSRQLVTVASQGDCGASKTGVAASGAEAEGCDEKALAVCGGNGSAANEPVCASDLVTYPSECEMVRRACTAQ 882 The following BLAST results are available for this feature:
BLAST of hypothetical protein DAPPUDRAFT_308094 vs. L. salmonis genes
Analysis Date: 2018-04-19 (T. kinsejongensis vs L. Salmonis peptides) Total hits: 21
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BLAST of hypothetical protein DAPPUDRAFT_308094 vs. SwissProt
Analysis Date: 2018-04-19 (T. kingejongensis peptided Blastp vs. SwissProt) Total hits: 25
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BLAST of hypothetical protein DAPPUDRAFT_308094 vs. nr
Analysis Date: 2018-05-15 (T. kingsejongensis proteins Blastp vs. NR) Total hits: 25
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The following features are aligned
Analyses
This gene is derived from or has results from the following analyses
Properties
Relationships
The following mRNA feature(s) are a part of this gene:
Sequences
The following sequences are available for this feature:
gene from alignment at scaffold421_size176100:44273..48605+ Legend: mRNA Hold the cursor over a type above to highlight its positions in the sequence below.>maker-scaffold421_size176100-snap-gene-0.22 ID=maker-scaffold421_size176100-snap-gene-0.22|Name=hypothetical protein DAPPUDRAFT_308094|organism=Tigriopus kingsejongensis|type=gene|length=4333bp|location=Sequence derived from alignment at scaffold421_size176100:44273..48605+ (Tigriopus kingsejongensis)back to top Synonyms
The feature 'hypothetical protein DAPPUDRAFT_308094' has the following synonyms
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